title: Antibody proteolysis: a common picture emerging from plants.
abstract: We have recently characterized the degradation profiles of 2 human IgG1 monoclonal antibodies, the tumor-targeting mAb H10 and the anti-HIV mAb 2G12. Both mAbs were produced in plants either as stable transgenics or using a transient expression system based on leaf agroinfiltration. The purified antibodies were separated by 1DE and protein bands were characterized by N-terminal sequencing. The proteolytic cleavage sites identified in the heavy chain (HC) of both antibodies were localized in 3 inter-domain regions, suggesting that the number of proteolytic cleavage events taking place in plants is limited. One of the cleavage sites, close to the hinge region, was common to both antibodies.
authors.affiliation: a Laboratory of Biotechnology; ENEA Research Center Casaccia ; Rome , Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Donini
authors.affiliation: a Laboratory of Biotechnology; ENEA Research Center Casaccia ; Rome , Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Lombardi
authors.affiliation: a Laboratory of Biotechnology; ENEA Research Center Casaccia ; Rome , Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Lonoce
authors.affiliation: a Laboratory of Biotechnology; ENEA Research Center Casaccia ; Rome , Italy.
authors.forename: Mariasole
authors.initials: M
authors.lastname: Di Carli
authors.affiliation: a Laboratory of Biotechnology; ENEA Research Center Casaccia ; Rome , Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Marusic
authors.affiliation: b National Research Council; Institute of Molecular Biology and Pathology ; Rome , Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Morea
authors.affiliation: c Department of Radiology, Oncology and Pathology ; Sapienza University of Rome ; Rome , Italy.
authors.forename: Patrizio
authors.initials: P
authors.lastname: Di Micco
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D006801:Humans; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D059748:Proteolysis
chemical_list: D000911:Antibodies, Monoclonal
keywords: 2DE; Agrobacterium tumefaciens; N-terminal sequencing; agroinfiltration; immunoglobulin; molecular farming; plant proteases; plant proteolysis