title: Lupeol Protects Against Cerulein-Induced Acute Pancreatitis in Mice.
abstract: Lupeol is a triterpenoid commonly found in fruits and vegetables and is known to exhibit a wide range of biological activities, including antiinflammatory and anti-cancer effects. However, the effects of lupeol on acute pancreatitis specifically have not been well characterized. Here, we investigated the effects of lupeol on cerulein-induced acute pancreatitis in mice. Acute pancreatitis was induced via an intraperitoneal injection of cerulein (50 µg/kg). In the lupeol treatment group, lupeol was administered intraperitoneally (10, 25, or 50 mg/kg) 1 h before the first cerulein injection. Blood samples were taken to determine serum cytokine and amylase levels. The pancreas was rapidly removed for morphological examination and used in the myeloperoxidase assay, trypsin activity assay, and real-time reverse transcription polymerase chain reaction. In addition, we isolated pancreatic acinar cells using a collagenase method to examine the acinar cell viability. Lupeol administration significantly attenuated the severity of pancreatitis, as was shown by reduced pancreatic edema, and neutrophil infiltration. In addition, lupeol inhibited elevation of digestive enzymes and cytokine levels, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and interleukin (IL)-6. Furthermore, lupeol inhibited the cerulein-induced acinar cell death. In conclusion, these results suggest that lupeol exhibits protective effects on cerulein-induced acute pancreatitis.
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Gi-Sang
authors.initials: GS
authors.lastname: Bae
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Sun Bok
authors.initials: SB
authors.lastname: Choi
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Il-Joo
authors.initials: IJ
authors.lastname: Jo
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Dong-Goo
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Joon-Yeon
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Sung-Kon
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Myoung-Jin
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Song
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.|Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749, Republic of Korea.
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Park
mesh_terms: D000208:Acute Disease; D000681:Amylases; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002108:Ceruletide; D016207:Cytokines / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008049:Lipase / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D053978:Pentacyclic Triterpenes / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000094042:Lupanes
chemical_list: D000681:Amylases; D000893:Anti-Inflammatory Agents; D002108:Ceruletide; D016207:Cytokines; D008049:Lipase; D053978:Pentacyclic Triterpenes; D009195:Peroxidase; D010936:Plant Extracts; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C439524:TNF protein, human; C010480:lupeol; D000094042:Lupanes
keywords: acute pancreatitis; cerulein; cytokines; inflammation; lupeol