title: Detection of Identical Isolates of Enterococcus faecalis from the Blood and Oral Mucosa in a Patient with Infective Endocarditis.
abstract: The detection of infective endocarditis (IE) of oral origin has been previously discussed. However, there are few reports confirming this infection using molecular biological techniques. We herein describe the case of a 67-year-old man who developed IE. Blood culture samples and strains obtained from the gingival and buccal mucosa showed 100% identity to Enterococcus faecalis JCM 5803 on sequencing of 16S rRNA gene fragments. A random amplification of polymorphic DNA (RAPD) analysis showed the same pattern for these samples, thus confirming the identity of E. faecalis isolates in the blood and oral mucosa. Our observations provide novel information regarding the level of identity between IE pathogens and oral bacteria.
authors.affiliation: Division of Hospital Dentistry, Central Clinical Department, Okayama University Hospital, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Okui
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Soga
authors.forename: Susumu
authors.initials: S
authors.lastname: Kokeguchi
authors.forename: Motoko
authors.initials: M
authors.lastname: Nose
authors.forename: Reiko
authors.initials: R
authors.lastname: Yamanaka
authors.forename: Nobuchika
authors.initials: N
authors.lastname: Kusano
authors.forename: Manabu
authors.initials: M
authors.lastname: Morita
mesh_terms: D000368:Aged; D001021:Aortic Valve / Q000473:pathology* / Q000601:surgery; D003114:Colony-Forming Units Assay; D004697:Endocarditis, Bacterial / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology*; D010138:Pacemaker, Artificial / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity; D065467:Transcatheter Aortic Valve Replacement / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D012336:RNA, Ribosomal, 16S