title: Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles.
abstract: Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide-based Cathepsin B cleavable sensor over a large array of nano-needles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma.
authors.affiliation: Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
authors.forename: Ciro
authors.initials: C
authors.lastname: Chiappini
authors.affiliation: Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
authors.forename: Paola
authors.initials: P
authors.lastname: Campagnolo
authors.affiliation: Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
authors.forename: Carina S
authors.initials: CS
authors.lastname: Almeida
authors.affiliation: Department of Surgery and Cancer, Imperial College London, W2 1PG, London, UK.
authors.forename: Nima
authors.initials: N
authors.lastname: Abbassi-Ghadi
authors.affiliation: Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
authors.forename: Lesley W
authors.initials: LW
authors.lastname: Chow
authors.affiliation: Department of Surgery and Cancer, Imperial College London, W2 1PG, London, UK.
authors.forename: George B
authors.initials: GB
authors.lastname: Hanna
authors.affiliation: Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
authors.forename: Molly M
authors.initials: MM
authors.lastname: Stevens
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002401:Cathepsin B / Q000378:metabolism*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000201:enzymology*; D004947:Esophagus / Q000166:cytology*; D006801:Humans; D009092:Mucous Membrane / Q000166:cytology; D036103:Nanotechnology / Q000379:methods*; D016062:Porosity; D012825:Silicon / Q000737:chemistry*
chemical_list: C533912:CTSB protein, human; D002401:Cathepsin B; D012825:Silicon
keywords: diagnostics; intracellular sensing; nanomedicine; nanoneedles; porous silicon