title: Determination and Pharmacokinetic Study of Apatinib in Rat Plasma by UPLC.
abstract: In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of apatinib in rat plasma using carbamazepine as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 µm particle size) and total run time was 2.0 min. The method was linear over the concentration range 5-1000 ng/mL with a lower limit of quantification of 5 ng/mL. Inter- and intraday precision were all within 6.5% and the accuracy was ≤3.5%. Recoveries of apatinib and IS were >80%. Stability studies showed that apatinib was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of apatinib to rats.
authors.affiliation: The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lou
authors.affiliation: The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China.
authors.forename: Li-man
authors.initials: LM
authors.lastname: Qiao
authors.affiliation: School of Pharmacy of Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Cheng
authors.affiliation: The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Pharmacy of Wenzhou Medical University, Wenzhou 325035, PR China hgxwzmu@163.com.
authors.forename: Guo-xin
authors.initials: GX
authors.lastname: Hu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D011725:Pyridines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D011725:Pyridines; C553458:apatinib