title: Association between polymorphisms of platelet membrane glycoprotein Ibα and risk of coronary heart disease in Han Chinese, Henan, China.
abstract: OBJECTIVE
  To study the relationship between human platelet alloantigens-2 (HPA-2) polymorphism, Kozak sequence polymorphism, macroglycopeptide region variable number of tandem repeats (VNTR) polymorphism of GPIbα and coronary heart disease (CHD).
  
  
  METHODS
  In the present study, blood obtained from 403 patients with CHD and 500 healthy controls was detected by PCR or PCR-RFLP methods to analyze the genotypes of HPA-2, Kozak sequence and VNTR.
  
  
  RESULTS
  About HPA-2 polymorphism, there were significant differences between CHD group and control group in TM+MM genotype (13.15% vs. 8.60%, P<0.05; OR 1.609; 95% CI 1.051 to 2.463) and M alleles distributions (6.58% vs. 4.40%, P<0.05; OR 1.645; 95% CI 1.090 to 2.482). For Kozak sequence polymorphism, between control group and CHD group, the difference of CC genotype distribution is statistic significance (3.20% vs. 7.69%, P<0.05; OR 2.000; 95% CI 1.076 to 3.718). The genotype analysis of VNTR in Han People of Henan (AC, BC, BD, CC, CD and DD) proved that no association between any genotypes or alleles and CHD. There weren't any significant differences about haplotypes of these genes between control group and CHD group (P>0.05).
  
  
  CONCLUSIONS
  The M allele of HPA-2 could be an important risk factor for CHD; the CC genotype of Kozak sequence would be a biomarker of genetic susceptibility about CHD; and each genotype of VNTR is no associated with CHD. No significant differences between control group and CHD group about haplotypes of these genes.
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics Orthopedics, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Peisheng
authors.initials: P
authors.lastname: Lv
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Guizhi
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Du
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Youyou
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Cardiology, The Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Luosha
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000328:Adult; D016824:Antigens, Human Platelet / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D003327:Coronary Disease / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D016017:Odds Ratio; D010641:Phenotype; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D016824:Antigens, Human Platelet; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor
keywords: CHD; HPA-2; Kozak sequence; VNTR; polymorphism