title: [Synthesis of core-shell hydrophilic polymer-silica hybrid material and its application in N-glycan enrichment].
abstract: Protein N-glycosylation is one of the most important post-translational modifications closely correlated with many important biological and pathological processes. The structural alterations of N-linked glycans in glycoproteins are always associated with many diseases, such as diabetes, heart failure and malignant tumors. Therefore, it is very important to establish sensitive methods for high-throughput N-glycan profiling. However, the low abundance of the N-glycoproteins and the heterogeneity of the N-glycans make it a challenge to analyse the protein glycosylation sensitively. In this work, we had synthesized core-shell hydrophilic polymer-silica hybrid materials (pGMAG-SiO2) for the efficient enrichment of protein N-glycans. Firstly, pGMAG-SiO2 was prepared by in situ growth of glucose polymer on the surface of silica microparticles using surface-initiated atom transfer radical polymerization (SI-ATRP) technique. The strong hydrophilicity of the material makes it suitable for the enrichment of N-glycans released from complex samples. Secondly, maltoheptaose and the N-glycans from chicken egg albumin were used as standard samples to optimize the enrichment conditions and evaluate the enrichment efficiency of pGMAG-SiO2. Finally, pGMAG-SiO2 was applied to the enrichment of N-linked glycans from human plasma proteins and 47 glycoforms were successfully identified after enrichment. These results demonstrated the high enrichment efficiency and significant application value of pGMAG-SiO2 in the analysis of N-glycans.
authors.forename: Haihong
authors.initials: H
authors.lastname: Bai
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
authors.forename: Bingquan
authors.initials: B
authors.lastname: Shen
authors.forename: Fang
authors.initials: F
authors.lastname: Tian
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
authors.forename: Yiting
authors.initials: Y
authors.lastname: Pan
authors.forename: Weijie
authors.initials: W
authors.lastname: Qin
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
mesh_terms: D001798:Blood Proteins / Q000737:chemistry; D005936:Glucans / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D011134:Polysaccharides; D011499:Protein Processing, Post-Translational; D012822:Silicon Dioxide
chemical_list: D001798:Blood Proteins; D005936:Glucans; D006023:Glycoproteins; D011108:Polymers; D011134:Polysaccharides; D012822:Silicon Dioxide