title: Anditalea andensis ANESC-ST--An Alkaliphilic Halotolerant Bacterium Capable of Electricity Generation under Alkaline-Saline Conditions.
abstract: A great challenge in wastewater bioremediation is the sustained activity of viable microorganisms, which can contribute to the breakdown of waste contaminants, especially in alkaline pH conditions. Identification of extremophiles with bioremediation capability can improve the efficiency of wastewater treatment. Here, we report the discovery of an electrochemically active alkaliphilic halotolerant bacterium, Anditalea andensis ANESC-ST (=CICC10485T=NCCB 100412T), which is capable of generating bioelectricity in alkaline-saline conditions. A. andensis ANESC-ST was shown to grow in alkaline conditions between pH 7.0-11.0 and also under high salt condition (up to 4 wt% NaCl). Electrical output was further demonstrated in microbial fuel cells (MFCs) with an average current density of ~0.5 µA/cm2, even under the harsh condition of 4 wt% NaCl and pH 9.0. Subsequent introduction of secreted extracellular metabolites into MFCs inoculated with Escherichia coli or Pseudomonas aeruginosa yielded enhanced electrical output. The ability of A. andensis ANESC-ST to generate energy under alkaline-saline conditions points towards a solution for bioelectricity recovery from alkaline-saline wastewater. This is the first report of A.andensis ANESC-ST producing bioelectricity at high salt concentration and pH.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore; Key Laboratory of Flexible Electronics & Institute of Advanced Materials, National Jiangsu Synergistic Innovation Center for Advanced Materials, Nanjing Tech University, 30 South Puzhu Road, Nanjing, 211816, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, 637551, Singapore; School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Victor Bochuan
authors.initials: VB
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Cui-E
authors.initials: CE
authors.lastname: Zhao
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Qichun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, 637551, Singapore; School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Say Chye Joachim
authors.initials: SC
authors.lastname: Loo
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, 637551, Singapore; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Chenjie
authors.initials: C
authors.lastname: Xu
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D041964:Cytophagaceae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D004579:Electron Transport / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D012965:Sodium Chloride / Q000494:pharmacology*; D062065:Wastewater / Q000382:microbiology
chemical_list: D012965:Sodium Chloride; D062065:Wastewater