title: NMR signal enhancement by effective SABRE labeling of oligopeptides.
abstract: Signal amplification by reversible exchange (SABRE) can enhance nuclear magnetic resonance signals by several orders of magnitude. However, until now this was limited to a small number of model target molecules. Here, a new convenient method for SABRE activation applicable to a variety of synthetic model oligopeptides is demonstrated. For the first time, a highly SABRE-active pyridine-based biocompatible molecular framework is incorporated into synthetic oligopeptides. The SABRE activity is preserved, demonstrating the importance of such earmarking. Finally, a crucial exchange process responsible for SABRE activity is identified and discussed.
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw (Poland). tratajczyk@ichf.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ratajczyk
authors.affiliation: Technische Universität Darmstadt, Eduard Zintl Institut für Anorganische und Physikalische Chemie, Alarich-Weiss-Str. 8, 64287 Darmstadt (Germany).
authors.forename: Torsten
authors.initials: T
authors.lastname: Gutmann
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw (Poland).
authors.forename: Piotr
authors.initials: P
authors.lastname: Bernatowicz
authors.affiliation: Technische Universität Darmstadt, Eduard Zintl Institut für Anorganische und Physikalische Chemie, Alarich-Weiss-Str. 8, 64287 Darmstadt (Germany).
authors.forename: Gerd
authors.initials: G
authors.lastname: Buntkowsky
authors.affiliation: Venitur Sp. z o.o., Wawozowa 34 B, 31-752 Krakow (Poland).
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Frydel
authors.affiliation: University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw (Poland).
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Fedorczyk
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D011725:Pyridines
keywords: NMR spectroscopy; hyperpolarization; peptides; signal amplification by reversible exchange; signal enhancement