title: Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia-Reperfusion Injury in Mice.
abstract: Oxysophoridine (OSR) is a bioactive alkaloid extracted from the Sophora alopecuroides Linn. Our aim is to explore the potential anti-inflammation mechanism of OSR in cerebral ischemic injury. Mice were intraperitoneally pretreated with OSR (62.5, 125, and 250 mg/kg) or nimodipine (Nim) (6 mg/kg) for 7 days followed by cerebral ischemia. The inflammatory-related cytokines in cerebral ischemic hemisphere tissue were determined by immunohistochemistry staining, Western blot and enzyme-like immunosorbent assay (ELISA). OSR-treated groups observably suppressed the nuclear factor kappa B (NF-κB), intercellular adhesion molecule-1 (ICAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). OSR-treated group (250 mg/kg) markedly reduced the inflammatory-related protein prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8). Meanwhile, it dramatically increased the interleukin-10 (IL-10). Our study revealed that OSR protected neurons from ischemia-induced injury in mice by downregulating the proinflammatory cytokines and blocking the NF-κB pathway.
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
authors.affiliation: College of Nursing, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Yin-Ju
authors.initials: YJ
authors.lastname: Hao
authors.affiliation: Medical Sci-Tech Research Center, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Medical Sci-Tech Research Center, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China. yujq910315@163.com.|Ningxia Hui Medicines Collaborative Innovation Center, Yinchuan, 750004, China. yujq910315@163.com.|Department of Pharmacology, Ningxia Medical University and Ningxia Hui Medicines Collaborative Innovation Center, Yinchuan, Ningxia, China. yujq910315@163.com.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Yu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D018836:Inflammation Mediators / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D008813:Mice, Inbred ICR; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D016207:Cytokines; C090509:Icam1 protein, mouse; D018836:Inflammation Mediators; D018696:Neuroprotective Agents; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; C406983:oxysophoridine; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: NF-κB; anti-inflammation; cerebral ischemia; oxysophoridine