title: Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling.
abstract: Hedgehog is a morphogen essential for body patterning and proper embryonic development from flies to humans. Thought quiescent in adults, its inappropriate reactivation is associated with many disparate genetic and sporadic types of human cancers. Recent findings have demonstrated a key, yet unexpected, role of the Hedgehog signaling pathway in metabolic control. Here, we describe a panel of methods to determine and analyze cellular and organismal metabolic changes downstream of the Hedgehog signaling pathway.
authors.affiliation: Institute of Experimental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764, Neuherberg, Munich, Germany.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Teperino
authors.forename: John Andrew
authors.initials: JA
authors.lastname: Pospisilik
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D020013:Calcium Signaling; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004734:Energy Metabolism; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D053823:Hedgehog Proteins; D055372:AMP-Activated Protein Kinases