title: Characterization of Proteinuria in Dogue de Bordeaux Dogs, a Breed Predisposed to a Familial Glomerulonephropathy: A Retrospective Study.
abstract: Dogue de Bordeaux dog has been reported to be predisposed to a familial glomerulonephropathy that displays some morphological modifications reported in focal and segmental glomerulosclerosis. Prevalence of quantitatively abnormal renal proteinuria was recently reported to be 33% in this breed. The nature of the proteinuria was assessed by sodium dodecyl sulfate-agarose gel electrophoresis and determinations of urinary markers (urinary retinol-binding protein, urinary N-acetyl-β-glucosaminidase, urinary albumin and urinary immunoglobulin G) on stored specimens. Diagnostic performances of sodium dodecyl sulfate-agarose gel electrophoresis to identify dogs with elevated urinary biomarkers were assessed. Samples from 102 adult Dogue de Bordeaux dogs (47 non-proteinuric [urine protein-to-creatinine ratio ≤ 0.2], 20 borderline-proteinuric [0.2< urine protein-to-creatinine ratio ≤ 0.5] and 35 proteinuric dogs [urine protein-to-creatinine ratio >0.5]) were used, of which 2 were suffering from familial glomerulonephropathy. The electrophoretic protein patterns, for all but one proteinuric dog, were indicative of a glomerular origin and, in all dogs, the urinary albumin concentration related to creatinine concentration and the urinary immunoglobulin G concentration related to creatinine concentration were above the upper limit of the reference interval established for the breed. Sensitivity and specificity of sodium dodecyl sulfate-agarose gel electrophoresis identifying dogs with elevated urinary albumin concentration were 94% and 92%, respectively, while diagnostic performance of sodium dodecyl sulfate-agarose gel electrophoresis in detecting dogs with elevated urinary immunoglobulin G concentration yielded sensitivity and specificity of 90% and 74%, respectively. These results suggest that all proteinuric and some borderline-proteinuric Dogue de Bordeaux dogs likely have underlying glomerular lesions and that sodium dodecyl sulfate-agarose gel electrophoresis and urinary markers might be useful to screen dogs with borderline-proteinuria. Additional investigations are warranted to assess if these findings are related to the familial glomerulonephropathy.
authors.affiliation: Internal Medicine Unit, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse (INP-ENVT), Toulouse, France.
authors.forename: Rachel
authors.initials: R
authors.lastname: Lavoué
authors.affiliation: Université de Toulouse, UPS, INP, ENVT, UMS006, Laboratoire Central de Biologie Médicale, Toulouse, France; INSERM, UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Trumel
authors.affiliation: Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Pascale M Y
authors.initials: PM
authors.lastname: Smets
authors.affiliation: Université de Toulouse, UPS, INP, ENVT, UMS006, Laboratoire Central de Biologie Médicale, Toulouse, France; INSERM, UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Braun
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Padova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Aresu
authors.affiliation: Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Daminet
authors.affiliation: UMR 1331 Toxalim, INRA, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse (INP-ENVT), Toulouse, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Concordet
authors.affiliation: Université de Toulouse, UPS, INP, ENVT, UMS006, Laboratoire Central de Biologie Médicale, Toulouse, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Palanché
authors.affiliation: Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Peeters
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000652:urine; D001947:Breeding; D003404:Creatinine / Q000652:urine; D004198:Disease Susceptibility; D004283:Dog Diseases / Q000175:diagnosis* / Q000652:urine; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005923:Glomerulosclerosis, Focal Segmental / Q000150:complications / Q000175:diagnosis* / Q000652:urine / Q000662:veterinary*; D007074:Immunoglobulin G / Q000652:urine; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011507:Proteinuria / Q000150:complications / Q000175:diagnosis* / Q000652:urine / Q000662:veterinary*; D012177:Retinol-Binding Proteins / Q000652:urine; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D012177:Retinol-Binding Proteins; D003404:Creatinine; D000118:Acetylglucosaminidase