title: Proteomic Analysis to Identify Tightly-Bound Cell Wall Protein in Rice Calli.
abstract: Rice is a model plant widely used for basic and applied research programs. Plant cell wall proteins play key roles in a broad range of biological processes. However, presently, knowledge on the rice cell wall proteome is rudimentary in nature. In the present study, the tightly-bound cell wall proteome of rice callus cultured cells using sequential extraction protocols was developed using mass spectrometry and bioinformatics methods, leading to the identification of 1568 candidate proteins. Based on bioinformatics analyses, 389 classical rice cell wall proteins, possessing a signal peptide, and 334 putative non-classical cell wall proteins, lacking a signal peptide, were identified. By combining previously established rice cell wall protein databases with current data for the classical rice cell wall proteins, a comprehensive rice cell wall proteome, comprised of 496 proteins, was constructed. A comparative analysis of the rice and Arabidopsis cell wall proteomes revealed a high level of homology, suggesting a predominant conservation between monocot and eudicot cell wall proteins. This study importantly increased information on cell wall proteins, which serves for future functional analyses of these identified rice cell wall proteins.
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
authors.affiliation: Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Tae Kyung
authors.initials: TK
authors.lastname: Hyun
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Dhinesh
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Yeonggil
authors.initials: Y
authors.lastname: Rim
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Xiong Yan
authors.initials: XY
authors.lastname: Chen
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Jo
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Suwha
authors.initials: S
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Keun Woo
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
authors.affiliation: Department of Plant Biology, University of California, Davis, CA 95616, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Lucas
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Jae-Yean
authors.initials: JY
authors.lastname: Kim
mesh_terms: D017360:Arabidopsis; D002473:Cell Wall / Q000378:metabolism*; D002478:Cells, Cultured; D012275:Oryza / Q000166:cytology / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: MudPIT; callus; cell wall proteins; proteomics; rice