title: Knockdown of long non-coding RNA HOTAIR inhibits malignant biological behaviors of human glioma cells via modulation of miR-326.
abstract: Glioma is the most common and aggressive primary adult brain tumor. Long non-coding RNAs (lncRNAs) have important roles in a variety of biological properties of cancers. Here, we elucidated the function and the possible molecular mechanisms of lncRNA HOTAIR in human glioma U87 and U251 cell lines. Quantitative RT-PCR demonstrated that HOTAIR expression was up-regulated in glioma tissues and cell lines. Knockdown of HOTAIR exerted tumor-suppressive function in glioma cells. Further, HOTAIR was confirmed to be the target of miR-326 and miR-326 mediated the tumor-suppressive effects of HOTAIR knockdown on glioma cell lines. Moreover, over-expressed miR-326 reduced the FGF1 expression which played an oncogenic role in glioma by activating PI3K/AKT and MEK 1/2 pathways. In addition, the in vivo studies also supported the above findings. Taken together, knockdown of HOTAIR up-regulated miR-326 expression, and further inducing the decreased expression of FGF1, these results provided a comprehensive analysis of HOTAIR-miR-326-FGF1 axis in human glioma and provided a new potential therapeutic strategy for glioma treatment.
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang 110122, China.|Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110122, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ke
authors.affiliation: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Yi-long
authors.initials: YL
authors.lastname: Yao
authors.affiliation: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang 110122, China.|Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110122, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Yun-hui
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang 110122, China.|Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110122, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Xiao-bai
authors.initials: XB
authors.lastname: Liu
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang 110122, China.|Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110122, China.
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Department of Physiology, College of Basic Medicine, China Medical University, Shenyang 110122, China.
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang 110122, China.|Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110122, China.
authors.forename: Yi-xue
authors.initials: YX
authors.lastname: Xue
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D016220:Fibroblast Growth Factor 1 / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy / Q000379:methods*; D005910:Glioma / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
chemical_list: C561566:HOTAIR long untranslated RNA, human; C545322:MIRN326 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D016220:Fibroblast Growth Factor 1
keywords: FGF1; HOTAIR; glioma; lncRNA; miR-326