title: Bevacizumab induces A549 cell apoptosis through the mechanism of endoplasmic reticulum stress in vitro.
abstract: OBJECTIVE
  To observe the effect of bevacizumab on human A549 cells and explore its mechanism.
  
  
  METHODS
  After different concentrations (0 μM, 1 μM, 5 μM, 25 μM) of bevacizumab treating in A549 cells, CCK8 assay detect the impact of bevacizumab on A549 cell proliferation and flow cytometry determine the effect of bevacizumab on human A549 cells apoptosis. Real-time PCR and Western blotting detect the changing expression of the target gene (CHOP, caspase-4, IRE1, XBP-1) on mRNA and Protein level.
  
  
  RESULTS
  Treatment with bevacizumab for 24-hr have induced cell death in a does-dependent manner dramatically (P<0.05). In terms of the mRNA level, expression of XBP-1 has increased obviously in each group (1 μM, 5 μM, 25 μM) (P<0.01); the expression of CHOP (25 μM) and caspase-4 (5 μM) have increased slightly (P<0.05). In terms of the protein level, the expression of CHOP has increased obviously in each group (1 μM, 5 μM, 25 μM) when compared with the control group (0 μM) (P<0.05). As for caspase-4 (5 μM, 25 μM), the expression have increased slightly when compared with the control group (0 μM) (P<0.05).
  
  
  CONCLUSIONS
  Bevacizumab can induce A549 cell apoptosis through the mechanism of endoplasmic reticulum stress.
authors.affiliation: Department of Respiratory Medicine, Hunan Province Geriatric Hospital Changsha 410016, China ; Institute of Respiratory Disease, Hunan Province Geriatric Hospital Changsha 410016, China.
authors.forename: Li-Le
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Hunan Province Geriatric Hospital Changsha 410016, China ; Institute of Respiratory Disease, Hunan Province Geriatric Hospital Changsha 410016, China.
authors.forename: Rui-Cheng
authors.initials: RC
authors.lastname: Hu
authors.affiliation: Department of Respiratory Medicine, Hunan Province Geriatric Hospital Changsha 410016, China ; Institute of Respiratory Disease, Hunan Province Geriatric Hospital Changsha 410016, China.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Dai
authors.affiliation: Department of Respiratory Medicine, Hunan Province Geriatric Hospital Changsha 410016, China ; Institute of Respiratory Disease, Hunan Province Geriatric Hospital Changsha 410016, China.
authors.forename: Shuang-Xiang
authors.initials: SX
authors.lastname: Tan
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000068258:Bevacizumab / Q000494:pharmacology*; D053471:Caspases, Initiator / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000071701:Regulatory Factor X Transcription Factors; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071717:X-Box Binding Protein 1
chemical_list: D020533:Angiogenesis Inhibitors; C497249:DDIT3 protein, human; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605543:XBP1 protein, human; D051742:Transcription Factor CHOP; D000068258:Bevacizumab; C528229:ERN1 protein, human; D017346:Protein Serine-Threonine Kinases; D004722:Endoribonucleases; C093516:CASP4 protein, human; D053471:Caspases, Initiator
keywords: A549 cell; Bevacizumab; apoptosis; endoplasmic reticulum stress