title: Salvianolic acid A reverses the paclitaxel resistance and inhibits the migration and invasion abilities of human breast cancer cells by inactivating transgelin 2.
abstract: Multidrug resistance and tumor migration and invasion are the major obstacles to effective breast cancer chemotherapy, but the underlying molecular mechanisms remain unclear. This study investigated the potential of transgelin 2 and salvianolic acid A to modulate the resistance and the migration and invasion abilities of paclitaxel-resistant human breast cancer cells (MCF-7/PTX). MCF-7/PTX cells were found to exhibit not only a high degree of resistance to paclitaxel, but also strong migration and invasion abilities. Small interfering RNA-mediated knockdown of TAGLN2 sensitized the MCF-7/PTX cells to paclitaxel, and inhibited their migration and invasion abilities. In addition, we also observed that combined salvianolic acid A and paclitaxel treatment could reverse paclitaxel resistance, markedly inhibit tumor migration and invasion, and suppress the expression of transgelin 2 in MCF-7/PTX cells. These findings indicate that salvianolic acid A can reverse the paclitaxel resistance and inhibit the migration and invasion abilities of human breast cancer cells by down-regulating the expression of transgelin 2, and hence could be useful in breast cancer treatments.
authors.affiliation: a Department of Pharmacy ; The First Affiliated Hospital of Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: a Department of Pharmacy ; The First Affiliated Hospital of Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Siying
authors.initials: S
authors.lastname: Chen
authors.affiliation: a Department of Pharmacy ; The First Affiliated Hospital of Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Qianting
authors.initials: Q
authors.lastname: Yang
authors.affiliation: b Department of Pharmacy ; The People's Hospital of Bayingol Mongolian Autonomous Prefecture ; Korla , Xinjiang , PR China.
authors.forename: Jiangxia
authors.initials: J
authors.lastname: Cai
authors.affiliation: a Department of Pharmacy ; The First Affiliated Hospital of Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: a Department of Pharmacy ; The First Affiliated Hospital of Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: You
authors.affiliation: c School of Pharmacy; Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xing
authors.affiliation: a Department of Pharmacy ; The First Affiliated Hospital of Xi'an Jiaotong University ; Xi'an , Shaanxi , PR China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002109:Caffeic Acids / Q000494:pharmacology*; D002465:Cell Movement; D048430:Cell Shape / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D061986:MCF-7 Cells; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D017239:Paclitaxel / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002109:Caffeic Acids; D007773:Lactates; D008840:Microfilament Proteins; D009124:Muscle Proteins; C585387:Tagln2 protein, human; C066201:salvianolic acid A; D017239:Paclitaxel
keywords: breast cancer; invasion; migration; paclitaxel resistance; salvianolic acid A; transgelin 2