title: Arbuscular mycorrhizal symbiosis regulates physiology and performance of Digitaria eriantha plants subjected to abiotic stresses by modulating antioxidant and jasmonate levels.
abstract: This study evaluates antioxidant responses and jasmonate regulation in Digitaria eriantha cv. Sudafricana plants inoculated (AM) and non-inoculated (non-AM) with Rhizophagus irregularis and subjected to drought, cold, or salinity. Stomatal conductance, photosynthetic efficiency, biomass production, hydrogen peroxide accumulation, lipid peroxidation, antioxidants enzymes activities, and jasmonate levels were determined. Stomatal conductance and photosynthetic efficiency decreased in AM and non-AM plants under all stress conditions. However, AM plants subjected to drought, salinity, or non-stress conditions showed significantly higher stomatal conductance values. AM plants subjected to drought or non-stress conditions increased their shoot/root biomass ratios, whereas salinity and cold caused a decrease in these ratios. Hydrogen peroxide accumulation, which was high in non-AM plant roots under all treatments, increased significantly in non-AM plant shoots under cold stress and in AM plants under non-stress and drought conditions. Lipid peroxidation increased in the roots of all plants under drought conditions. In shoots, although lipid peroxidation decreased in AM plants under non-stress and cold conditions, it increased under drought and salinity. AM plants consistently showed high catalase (CAT) and ascorbate peroxidase (APX) activity under all treatments. By contrast, the glutathione reductase (GR) and superoxide dismutase (SOD) activity of AM roots was lower than that of non-AM plants and increased in shoots. The endogenous levels of cis-12-oxophytodienoc acid (OPDA), jasmonic acid (JA), and 12-OH-JA showed a significant increase in AM plants as compared to non-AM plants. 11-OH-JA content only increased in AM plants subjected to drought. Results show that D. eriantha is sensitive to drought, salinity, and cold stresses and that inoculation with AM fungi regulates its physiology and performance under such conditions, with antioxidants and jasmonates being involved in this process.
authors.affiliation: Laboratorio de Fisiología Vegetal. Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejército de los Andes 950, 5700, San Luis, Argentina. hepedra@unsl.edu.ar.
authors.forename: H
authors.initials: H
authors.lastname: Pedranzani
authors.affiliation: Laboratorio de Fisiología Vegetal. Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejército de los Andes 950, 5700, San Luis, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Rivera
authors.affiliation: Laboratorio de Fisiología Vegetal. Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejército de los Andes 950, 5700, San Luis, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Gutiérrez
authors.affiliation: Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Prof. Albareda, 1, E-18008, Granada, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Porcel
authors.affiliation: Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle Saale, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hause
authors.affiliation: Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Prof. Albareda, 1, E-18008, Granada, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Ruiz-Lozano
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D003080:Cold Temperature; D003517:Cyclopentanes / Q000378:metabolism*; D031746:Digitaria / Q000382:microbiology* / Q000502:physiology; D055864:Droughts; D055137:Glomeromycota / Q000502:physiology*; D038821:Mycorrhizae / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism*; D054712:Salinity; D013312:Stress, Physiological; D013559:Symbiosis
chemical_list: D000975:Antioxidants; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid
keywords: Arbuscular mycorrhiza; Cold stress; Digitaria eriantha; Drought stress; Jasmonates; Salt stress