title: Protein microarray with horseradish peroxidase chemiluminescence for quantification of serum α-fetoprotein.
abstract: OBJECTIVE
  To develop and evaluate a protein microarray assay with horseradish peroxidase (HRP) chemiluminescence for quantification of α-fetoprotein (AFP) in serum from patients with hepatocellular carcinoma (HCC).
  
  
  METHODS
  A protein microarray assay for AFP was developed. Serum was collected from patients with HCC and healthy control subjects. AFP was quantified using protein microarray and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Serum AFP concentrations determined via protein microarray were positively correlated (r = 0.973) with those determined via ELISA in patients with HCC (n = 60) and healthy control subjects (n = 30). Protein microarray showed 80% sensitivity and 100% specificity for HCC diagnosis. ELISA had 83.3% sensitivity and 100% specificity. Protein microarray effectively distinguished between patients with HCC and healthy control subjects (area under ROC curve 0.974; 95% CI 0.000, 1.000).
  
  
  CONCLUSIONS
  Protein microarray is a rapid, simple and low-cost alternative to ELISA for detecting AFP in human serum.
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Yuanshun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Lin
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Chengzeng
authors.initials: C
authors.lastname: Yin
authors.affiliation: Beijing Institute of Hepatology, Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Boxun
authors.initials: B
authors.lastname: Jin
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Sun
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Institute of Hepatology, Beijing You'an Hospital, Capital Medical University, Beijing, China liningya@ccmu.edu.cn.
authors.forename: Aiying
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Beijing You'an Hospital, Capital Medical University, Beijing, China Beijing Institute of Hepatology, Beijing You'an Hospital, Capital Medical University, Beijing, China liningya@ccmu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000509:alpha-Fetoproteins; D006735:Horseradish Peroxidase
keywords: hepatocellular carcinoma; horseradish peroxidase; protein microarray; α-fetoprotein