title: Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene.
abstract: Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca(2+), Mg(2+), K(+), and HCO3 (-) in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.
authors.affiliation: College of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
authors.forename: Ju-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: College of Tea & Food Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
authors.forename: Xiu-Heng
authors.initials: XH
authors.lastname: Xue
authors.affiliation: College of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Fan
authors.affiliation: College of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Xie
authors.affiliation: College of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D003457:Cryptosporidiosis / Q000469:parasitology; D003458:Cryptosporidium / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018528:ATP-Binding Cassette Transporters; D015800:Protozoan Proteins; D000255:Adenosine Triphosphate; D007501:Iron; D002118:Calcium
keywords: ATP-binding cassette transporter protein; Cryptosporidium andersoni; cloning; iron transportation