title: Anthelmintic activity of Artemisia vestita Wall ex DC. and Artemisia maritima L. against Haemonchus contortus from sheep.
abstract: Current study was designed to evaluate in vivo and in vitro anthelmintic activity of Artemisia vestita Wall ex DC. and Artemisia maritima L. against Haemonchus contortus in comparison with ivermectin to investigate the effect of plant extracts on survival of infective L3 and adults under in vitro condition. Plant extracts were given to H. contortus infected sheep orally and it was infected with L3 stage of H. contortus at dose of 5000 larvae/sheep. Total of 25-30 larvae were incubated with plant extracts in PBS alone and ivermectin at different concentration used as positive control. It was recorded that there is a significant decrease in fecal egg count (FEC) after post-treatment period for both plants. The highest fecal egg count reduction for A. vestita was 87.2% at 100mg/kg while for A. maritima it was 84.5% on day 28 post-treatment. Investigated extracts indicated significant activity against larvae and adult worms.
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan; Department of Zoology and Biology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Shamaila
authors.initials: S
authors.lastname: Irum
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Ahmed
authors.affiliation: Barani Livestock Production Research Institute, Kharimurat, Attock, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mukhtar
authors.affiliation: Barani Livestock Production Research Institute, Kharimurat, Attock, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mushtaq
authors.affiliation: Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Bushra
authors.initials: B
authors.lastname: Mirza
authors.affiliation: Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Donskow-Łysoniewska
authors.affiliation: Department of Zoology and Biology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Mazhar
authors.initials: M
authors.lastname: Qayyum
authors.affiliation: Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey. Electronic address: ssimsek@firat.edu.tr.
authors.forename: Sami
authors.initials: S
authors.lastname: Simsek
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006189:Haemonchus / Q000187:drug effects*; D007559:Ivermectin / Q000008:administration & dosage / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D007559:Ivermectin
keywords: Anthelmintic; Artemisia maritima; Artemisia vestita; Haemonchus contortus; Plants