title: Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.
abstract: Plant roots are important for a wide range of processes, including nutrient and water uptake, anchoring and mechanical support, storage functions, and as the major interface with the soil environment. Several small signalling peptides and receptor kinases have been shown to affect primary root growth, but very little is known about their role in lateral root development. In this context, the CLE family, a group of small signalling peptides that has been shown to affect a wide range of developmental processes, were the focus of this study. Here, the expression pattern during lateral root initiation for several CLE family members is explored and to what extent CLE1, CLE4, CLE7, CLE26, and CLE27, which show specific expression patterns in the root, are involved in regulating root architecture in Arabidopsis thaliana is assessed. Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling. In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed. Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.
authors.forename: Nathan
authors.initials: N
authors.lastname: Czyzewicz
authors.affiliation: Department of Biosciences, Section for Genetics and Evolutionary Biology, University of Oslo, N-0316 Oslo, Norway.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Shi
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium Department of Plant Biotechnology and Genetics, Ghent University, B-9052 Ghent, Belgium Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium.
authors.forename: Lam Dai
authors.initials: LD
authors.lastname: Vu
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium Department of Plant Biotechnology and Genetics, Ghent University, B-9052 Ghent, Belgium.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Van De Cotte
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.
authors.forename: Charlie
authors.initials: C
authors.lastname: Hodgman
authors.affiliation: Department of Biosciences, Section for Genetics and Evolutionary Biology, University of Oslo, N-0316 Oslo, Norway.
authors.forename: Melinka A
authors.initials: MA
authors.lastname: Butenko
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium Department of Plant Biotechnology and Genetics, Ghent University, B-9052 Ghent, Belgium Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK ive.desmet@psb.vib-ugent.be.
authors.forename: Ive
authors.initials: I
authors.lastname: De Smet
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D058431:Brachypodium / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D018517:Plant Roots / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000607729:CLE26 protein, Arabidopsis
keywords: Arabidopsis thaliana; Brachypodium distachyon; CLE26; auxin; root; wheat.