title: Is idiopathic hirsutism (IH) really idiopathic? mRNA expressions of skin steroidogenic enzymes in women with IH.
abstract: OBJECTIVE
  Hirsutism results from hyperandrogenemia and/or exaggerated androgen responsiveness. Among various causes of hirsutism, some patients do not exhibit androgen excess which is called idiopathic hirsutism (IH). The pathogenesis of IH could not so far be clearly established.
  
  
  METHODS
  To investigate the mRNA expression of aromatase enzyme and the other enzymes having functional roles in the steroidogenic pathway, in freshly obtained skin tissue from subumbilical skin and the arm of the patients with IH and healthy women.
  
  
  METHODS
  Twenty-one women with IH and 15 healthy women were included in the study. We aimed to determine mRNA expressions of genes associated with local androgen synthesis and metabolism (CYP11A1, STS, CYP19A1, SRD5A1, SRD5A2, HSD3B1, AR, COMT, ESR1, ESR2, HSD3B2, CYP17A1, SULT2A1, SULT1E1, HSD17B2, IL6, TGFB1, TNFA) from skin biopsy and blood samples of patients with IH and the data compared with healthy subjects.
  
  
  RESULTS
  Patients with IH exhibit significantly lower interleukin 6 (IL6) mRNA expression and higher steroid sulphatase (STS) and hydroxysteroid (17beta) dehydrogenase 2 (HSD17B2), gene mRNA expression, respectively, in the subumbilical region skin biopsies. Similarly, patients with IH exhibit significantly lower IL6 mRNA expression and higher STS and HSD17B2 gene mRNA expression, respectively, in the arm skin compared to healthy women's subumbilical region.
  
  
  CONCLUSIONS
  In both arm and subumbilical skin biopsy of patients with IH, we observed an up-regulation of HSD17B2 and STS, decreased IL6 mRNA expression, probably determining an increase in the local amount of active androgens, which could then be used as substrate for other androgen metabolic routes.
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Taheri
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Gokmen
authors.initials: G
authors.lastname: Zararsiz
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Sulbiye
authors.initials: S
authors.lastname: Karaburgu
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Borlu
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Mahmut Tuncay
authors.initials: MT
authors.lastname: Ozgun
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Zuleyha
authors.initials: Z
authors.lastname: Karaca
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Tanriverdi
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Munis
authors.initials: M
authors.lastname: Dundar
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey.
authors.forename: Fahrettin
authors.initials: F
authors.lastname: Kelestimur
authors.affiliation: Departments of Medical BiologyStatisticsEndocrinologyDermatologyObstetrics and GynecologyGeneticsErciyes University Medical School, Kayseri 38039, TurkeyBetul-Ziya Eren Genome and Stem Cell CenterErciyes University, Kayseri, Turkey kursad@erciyes.edu.tr.
authors.forename: Kursad
authors.initials: K
authors.lastname: Unluhizarci
mesh_terms: D000005:Abdomen; D000293:Adolescent; D000328:Adult; D000728:Androgens / Q000096:biosynthesis* / Q000378:metabolism; D001132:Arm; D016022:Case-Control Studies; D004960:Estradiol Dehydrogenases / Q000235:genetics; D005260:Female; D006628:Hirsutism / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood / Q000378:metabolism*; D012867:Skin / Q000201:enzymology / Q000378:metabolism*; D043266:Steryl-Sulfatase / Q000235:genetics; D055815:Young Adult
chemical_list: D000728:Androgens; C508600:IL6 protein, human; D015850:Interleukin-6; D012333:RNA, Messenger; D004960:Estradiol Dehydrogenases; C523763:HSD17B2 protein, human; C584039:STS protein, human; D043266:Steryl-Sulfatase