title: Toxicity study in blood and tumor cells of laser produced medicines for application in fabrics.
abstract: Phenothiazine derivatives are non-antibiotics with antimicrobial, fungistatic and fungicidal effects. We exposed to a high energy UV laser beam phenothiazines solutions in water at 20mg/mL concentration to increase antibacterial activity of resulting mixtures. Compared to previous results obtained on bacteria, more research is needed about UV laser irradiated phenothiazines applications on cancer cell cultures to evidence possible anticancerous properties. Evaluation of the safety of the newly obtained photoproducts in view of use on humans is also needed. Due to expensive animal testing in toxicology and pressure from general public and governments to develop alternatives to in vivo testing, in vitro cell-based models are attractive for preliminary testing of new materials. Cytotoxicity screening reported here shows that laser irradiated (4h exposure time length) chlorpromazine and promazine are more efficient against some cell cultures. Interaction of laser irradiated phenothiazines with fabrics show that promethazine and chlorpromazine have improved wetting properties. Correlation of these two groups of properties shows that chlorpromazine appears to be more recommended for applications on tissues using fabrics as transport vectors. The reported results concern stability study of phenothiazines water solutions to know the time limits within which they are stable and may be used.
authors.affiliation: Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain; Institut de Nanociència i Nanotecnologia-IN(2)UB, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: mcmoranb@ub.edu.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Morán
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Romania.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tozar
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Romania.
authors.forename: Agota
authors.initials: A
authors.lastname: Simon
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Romania.
authors.forename: Andra
authors.initials: A
authors.lastname: Dinache
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Smarandache
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania.
authors.forename: Ionut Relu
authors.initials: IR
authors.lastname: Andrei
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Romania.
authors.forename: Mihai
authors.initials: M
authors.lastname: Boni
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Romania.
authors.forename: Mihail Lucian
authors.initials: ML
authors.lastname: Pascu
authors.affiliation: CNR - Istituto per l' Energetica e le Interfasi, 16149 Genova, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cirisano
authors.affiliation: CNR - Istituto per l' Energetica e le Interfasi, 16149 Genova, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Ferrari
mesh_terms: D016475:3T3 Cells; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007834:Lasers; D051379:Mice; D010640:Phenothiazines / Q000008:administration & dosage / Q000633:toxicity*; D013784:Textiles
chemical_list: D010640:Phenothiazines
keywords: Culture cells; Fabrics; Hemolysis; In vitro cytotoxicity; Laser; Phenothiazines