title: An Environmentally Friendly and Efficient Method for Extraction of PHB Biopolymer with Non-Halogenated Solvents.
abstract: The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25 °C until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103 °C and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 10(6) compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.
authors.affiliation: Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran 16765-3454, Iran.
authors.forename: Asieh
authors.initials: A
authors.lastname: Aramvash
authors.affiliation: Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran 16765-3454, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Gholami-Banadkuki
authors.affiliation: Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran 16765-3454, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moazzeni-Zavareh
authors.affiliation: Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran 16765-3454, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Hajizadeh-Turchi
mesh_terms: D000085:Acetates; D020118:Cupriavidus necator / Q000737:chemistry*; D006358:Hot Temperature; D006885:Hydroxybutyrates / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents; D000094122:Polyhydroxybutyrates
chemical_list: D000085:Acetates; D006885:Hydroxybutyrates; D011091:Polyesters; D012997:Solvents; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C006848:butyl acetate; C007650:ethyl acetate
keywords: Butyl acetate; Central composite design; Cupriavidus necator; Design expert; Ethyl acetate; Response Surface methodology