title: RAP-PCR fingerprinting reveals time-dependent expression of development-related genes following differentiation process of Bacillus thuringiensis.
abstract: Gene expression profiles are important data to reveal the functions of genes putatively involved in crucial biological processes. RNA arbitrarily primed polymerase chain reaction (RAP-PCR) and specifically primed reverse transcription polymerase chain reaction (RT-PCR) were combined to screen differentially expressed genes following development of a commercial Bacillus thuringiensis subsp. kurstaki strain 8010 (serotype 3a3b). Six differentially expressed transcripts (RAP1 to RAP6) were obtained. RAP1 encoded a putative triple helix repeat-containing collagen or an exosporium protein H related to spore pathogenicity. RAP2 was homologous to a ClpX protease and an ATP-dependent protease La (LonB), which likely acted as virulence factors. RAP3 was homologous to a beta subunit of propionyl-CoA carboxylase required for the development of Myxococcus xanthus. RAP4 had homology to a quinone oxidoreductase involved in electron transport and ATP formation. RAP5 showed significant homology to a uridine kinase that mediates phosphorylation of uridine and azauridine. RAP6 shared high sequence identity with 3-methyl-2-oxobutanoate-hydroxymethyltransferase (also known as ketopantoate hydroxymethyltransferase or PanB) involved in the operation of the tricarboxylic acid cycle. The findings described here would help to elucidate the molecular mechanisms underlying the differentiation process of B. thuringiensis and unravel novel pathogenic genes.
authors.affiliation: a Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, People's Republic of China.|b Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, 350002 Fuzhou, Fujian, People's Republic of China.
authors.forename: Tianpei
authors.initials: T
authors.lastname: Huang
authors.affiliation: a Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, People's Republic of China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yu
authors.affiliation: c Biological Centre of the Czech Academy of Sciences, Institute of Entomology, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Gelbič
authors.affiliation: a Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, People's Republic of China.|b Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, 350002 Fuzhou, Fujian, People's Republic of China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
mesh_terms: D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016172:DNA Fingerprinting / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013171:Spores, Bacterial / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins
keywords: Bacillus thuringiensis; PCR de l’ARN utilisant des amorces arbitraires; RNA arbitrarily primed PCR; cycle vital; development; differentially expressed gene; développement; gène exprimé différentiellement; life cycle