title: New insights into coenzyme A interaction with mercury ions provided by mass spectrometric and circular dichroism spectroscopic approaches.
abstract: The interaction of coenzyme A (CoA) with mercury ions was investigated using electrospray ionization mass spectrometry and circular dichroism spectroscopy. Our results indicated a 1:1 stoichiometric CoA-Hg complex at physiological pH. Furthermore, the CoA conformation considerably changed in the presence of mercury ions. In addition, a by-product of the reaction, thiocoenzyme A, was identified using mass spectrometry.
authors.affiliation: Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi-700506, Romania.. andries.claudia17@gmail.com.
authors.forename: Claudia
authors.initials: C
authors.lastname: Andries
authors.affiliation: University of Konstanz, Department of Chemistry and Zukunftskolleg, 78457 Konstanz, Germany.. marilena.manea@uni- konstanz.de.
authors.forename: Marilena
authors.initials: M
authors.lastname: Manea
authors.affiliation: Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi-700506, Romania.. gabidr@uaic.ro.
authors.forename: Gabi
authors.initials: G
authors.lastname: Drochioiu
authors.affiliation: Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi-700506, Romania.. robert.gradinaru@uaic.ro.
authors.forename: Robert
authors.initials: R
authors.lastname: Gradinaru