title: Simultaneous detection of peripheral mononuclear cell and plasma tissue factor expression for prevention and treatment of ischemic cardiocerebrovascular diseases.
abstract: OBJECTIVE
  This study aimed to simultaneously observe the expression of mononuclear cells (Mo) and plasma tissue factor (TF) in patients with ischemic cardiocerebrovascular diseases during the stage of acute onset and after the following three weeks and three months for exploration of the clinical implications concerned.
  
  
  METHODS
  MoTF mRNA and plasma TF antigen (TFAg) from 76 patients with acute myocardial infarction (AMI) together with 46 patients with acute ischemic stroke (AIS) and 61 healthy controls were quantitated respectively through RT-PCR and ELISA.
  
  
  RESULTS
  Compared with the results in the control group, the level of MoTFmRNA and plasma TF in the other groups increased simultaneously and dramatically in the acute stage, which showed a good correlation among the groups (P<0.01), especially in AIS group. The quantitative data showed that both MoTF mRNA and plasma TF remained higher than that of the control group (P<0.01 and P<0.05) after three weeks from the acute onset. It was after three months that the content of MoTF mRNA, in spite of its relatively high level (P<0.05), began to decline in AMI and AIS groups. In this stage the level of MoTFmRNA in AIS group was lower than that in the acute onset stage (P<0.05), while the reduction of plasma TF in AMI and AIS groups was not significantly different from that of the control group (P>0.05). However, the reduced level of plasma TF was still different from that in the acute onset stage (P<0.05).
  
  
  CONCLUSIONS
  The simultaneous increase of the level of peripheral MoTF mRNA and plasma TF in the acute onset stage of ischemic cardiocerebrovascular diseases shows a good correlation and suggests the up-regulation of MoTF mRNA's expression participates in the maintenance and expansion of thrombotic formation. Dynamic monitoring of MoTF mRNA and plasma TF at different time points after acute onset has important clinical implications for prevention and treatment of arterial thrombotic diseases.
authors.affiliation: Yangpu District Central Hospital Affiliated to Tongji University School of Medicine Shanghai 200090, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Institute for Heart Disease Research of The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, China.
authors.forename: Yi-Tong
authors.initials: YT
authors.lastname: Ma
authors.affiliation: Yangpu District Central Hospital Affiliated to Tongji University School of Medicine Shanghai 200090, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ma
authors.affiliation: Yangpu District Central Hospital Affiliated to Tongji University School of Medicine Shanghai 200090, China.
authors.forename: De
authors.initials: D
authors.lastname: Chen
authors.affiliation: Yangpu District Central Hospital Affiliated to Tongji University School of Medicine Shanghai 200090, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Institute for Heart Disease Research of The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, China.
authors.forename: Bing-Zhao
authors.initials: BZ
authors.lastname: Wen
authors.affiliation: Department of Vascular Biology, Central South University Xiangya School of Medicine Changsha 410078, China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002561:Cerebrovascular Disorders / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020521:Stroke / Q000378:metabolism*; D013925:Thromboplastin / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D013925:Thromboplastin
keywords: Myocardial infarction; brain ischemia; monocytes; tissue factor (TF)