title: G1 cyclin driven DNA replication.
abstract: The mitotic cell cycle is driven by Cyclin-Dependent Kinases (CDK). CDK activation requires the binding of activatory subunits termed cyclins. Different waves of cyclins are expressed during the cell cycle, enabling CDKs to trigger phase specific events. For instance, S phase cyclins promote the initiation of DNA replication but not chromosome segregation. There are at least 2 explanations for how such regulation is achieved. According to one of the visions, cyclins confer intrinsic substrate specificity to the CDK catalytic subunit. Alternatively a quantitative model has been proposed, according to which ever-increasing CDK activity is required to trigger cell cycle events from G1 to M. If a quantitative control prevails, then an early cyclin should trigger later cycle events if accumulated at high enough levels at the right time and place. We show here that a G1 phase cyclin bears the potential to trigger DNA replication and promote S and G2 phase specific transcription.
authors.affiliation: a Department of Biochemistry and Molecular Biology ; Biophysics Unit, School of Medicine; Universitat Autonoma de Barcelona ; Bellaterra , Catalonia , Spain.
authors.forename: Roger
authors.initials: R
authors.lastname: Palou
authors.affiliation: a Department of Biochemistry and Molecular Biology ; Biophysics Unit, School of Medicine; Universitat Autonoma de Barcelona ; Bellaterra , Catalonia , Spain.
authors.forename: Asrar
authors.initials: A
authors.lastname: Malik
authors.affiliation: a Department of Biochemistry and Molecular Biology ; Biophysics Unit, School of Medicine; Universitat Autonoma de Barcelona ; Bellaterra , Catalonia , Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Palou
authors.affiliation: a Department of Biochemistry and Molecular Biology ; Biophysics Unit, School of Medicine; Universitat Autonoma de Barcelona ; Bellaterra , Catalonia , Spain.
authors.forename: Fanli
authors.initials: F
authors.lastname: Zeng
authors.affiliation: a Department of Biochemistry and Molecular Biology ; Biophysics Unit, School of Medicine; Universitat Autonoma de Barcelona ; Bellaterra , Catalonia , Spain.
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.affiliation: a Department of Biochemistry and Molecular Biology ; Biophysics Unit, School of Medicine; Universitat Autonoma de Barcelona ; Bellaterra , Catalonia , Spain.
authors.forename: David G
authors.initials: DG
authors.lastname: Quintana
mesh_terms: D015153:Blotting, Western; D002455:Cell Division / Q000235:genetics; D056766:Cyclin G1 / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D005434:Flow Cytometry; D016193:G1 Phase / Q000235:genetics; D008856:Microscopy, Fluorescence; D013379:Substrate Specificity
chemical_list: D056766:Cyclin G1; D018844:Cyclin-Dependent Kinases
keywords: Cdk1; cyclin; orderly phase progression; quantitative mode; replication; substrate specificity