title: RP-HPLC-DAD-MS(n) Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts.
abstract: High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3-O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's method. All extracts inhibited BuChE in a concentration-dependent form; however, the methanol extract showed a better effect that the other extracts. These compounds have been identified previously in the Velloziaceae family, but for the first time as constituents of B. blanchetii.
authors.forename: Jósquia S
authors.initials: JS
authors.lastname: Barbosa
authors.forename: Verônica M
authors.initials: VM
authors.lastname: Almeida
authors.forename: Rosilene M
authors.initials: RM
authors.lastname: Marçal
authors.forename: Alexsandro
authors.initials: A
authors.lastname: Branco
mesh_terms: D002091:Butyrylcholinesterase / Q000032:analysis; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D002091:Butyrylcholinesterase