title: Immunogenicity of porcine P[6], P[7]-specific △VP8* rotavirus subunit vaccines with a tetanus toxoid universal T cell epitope.
abstract: Currently, commercial porcine rotavirus vaccines remain varied limitations. The objective of this study is to develop an alternative porcine rotavirus subunit vaccine candidate by parenteral administration, which enables to elicit robust immune responses against most prevalence porcine rotavirus strains. The bacterially-expressed porcine rotavirus P[6]- or P[7]-specific truncated VP8* (aa 64-223) recombinant protein with or without a universal tetanus toxoid CD4(+) T cell epitope P2 was generated. All the recombinant subunit proteins △VP8*s or P2-△VP8*s were of high solubility and high yields. The immunogenicity of each purified △VP8* and P2-△VP8* was evaluated in mice (10 μg/dose) or guinea pigs (20 μg/dose) immunized IM with 600 μg aluminum hydroxide three times at 2-week interval. The introduction of P2T cell epitope to P[7]-△VP8* elicited significantly higher IgG titer in mice than its absence. Comparatively, P2 epitope slightly enhanced the immunogenicity of P[6]-△VP8*. P2-P[7]△VP8* elicited high titer of neutralizing antibody against heterotypic P[7]-specific rotaviruses with varied G type combination. Our data indicated that two subunit vaccines could be plausible bivalent rotavirus vaccine candidate to provide antigenic coverage of porcine rotavirus strains of global or regional importance.
authors.affiliation: College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wen
authors.affiliation: College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China; Hong Kong Baptist University, Changshu Research Institute, Suzhou 215000, Jiangsu Province, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Wei
authors.affiliation: College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China. Electronic address: ranxuhua@163.com.
authors.forename: Xuhua
authors.initials: X
authors.lastname: Ran
authors.affiliation: College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Ni
authors.affiliation: College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
authors.forename: Si
authors.initials: S
authors.lastname: Cao
authors.affiliation: College of Animal Science & Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage*; D005260:Female; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D008807:Mice, Inbred BALB C; D022243:Rotavirus Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D013745:Tetanus Toxoid / Q000008:administration & dosage*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D018984:Epitopes, T-Lymphocyte; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; D013745:Tetanus Toxoid; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D000536:Aluminum Hydroxide
keywords: P type; Porcine rotavirus; Subunit vaccine; T cell epitope; Tetanus toxoid; VP8* protein