title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance.
abstract: Transcription factors (TFs) play important roles in plant growth, development, and responses to environmental stress. In this study, we collected 1,455 full-length (FL) cDNAs of TFs, representing 45 families, from wheat and its relatives Triticum urartu, Aegilops speltoides, Aegilops tauschii, Triticum carthlicum, and Triticum aestivum. More than 15,000 T0 TF FOX (Full-length cDNA Over-eXpressing) rice lines were generated; of these, 10,496 lines set seeds. About 14.88% of the T0 plants showed obvious phenotypic changes. T1 lines (5,232 lines) were screened for salt and osmotic stress tolerance using 150 mM NaCl and 20% (v/v) PEG-4000, respectively. Among them, five lines (591, 746, 1647, 1812, and J4065) showed enhanced salt stress tolerance, five lines (591, 746, 898, 1078, and 1647) showed enhanced osmotic stress tolerance, and three lines (591, 746, and 1647) showed both salt and osmotic stress tolerance. Further analysis of the T-DNA flanking sequences showed that line 746 over-expressed TaEREB1, line 898 over-expressed TabZIPD, and lines 1812 and J4065 over-expressed TaOBF1a and TaOBF1b, respectively. The enhanced salt and osmotic stress tolerance of lines 898 and 1812 was confirmed by retransformation of the respective genes. Our results demonstrate that a heterologous FOX system may be used as an alternative genetic resource for the systematic functional analysis of the wheat genome.
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Wu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Butuo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Zhanpeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Longzhi
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Jizeng
authors.initials: J
authors.lastname: Jia
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Sun
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000254:growth & development* / Q000378:metabolism; D009997:Osmotic Pressure; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D055049:Salt Tolerance / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D010940:Plant Proteins; C036483:T-DNA; D014157:Transcription Factors; D012965:Sodium Chloride