title: The use of serum glial fibrillary acidic protein test as a promising tool for intracerebral hemorrhage diagnosis in Chinese patients and prediction of the short-term functional outcomes.
abstract: The objective of this study was to explore the efficacy of glial fibrillary acidic protein (GFAP) in differentiating intracerebral hemorrhage (ICH) from ischemic stroke (IS). Suspicious patients of acute stroke were screened and finally diagnosed by computed tomography and magnetic resonance imaging. Blood samples were collected within 2-6 h after onset of symptoms, and serum GFAP level was determined by ELISA assay. The functional outcome for the patients was determined by modified Rankin Scale (mRS) 90 days after onset of symptoms. 43 ICH patients and 65 IS patients were enrolled. GFAP concentration in ICH group was significantly higher than in IS group (p < 0.001). Significant correlation was found when comparing GFAP with National Institutes of Health Stroke Scale (NIHSS) (r = 0.418, p = 0.005) and hemorrhage volume (r = 0.840, p < 0.001) in ICH group, while such correlation was not observed in IS group. ROC analysis indicated that GFAP level at the cut-point of 0.7 ng/ml yielded an AUC of 0.901 (95 % CI 0.828-0.950) with high sensitivity (86.0 %) and specificity (76.9 %) to differentiate ICH from IS. Patients with higher serum GFAP concentration in ICH group experienced poorer functional disability (r = 0.755, p < 0.001), while this phenomenon was not observed in IS group (r = -0.114, p = 0.368). ROC curve analysis found that GFAP level at the cut-point of 1.04 ng/ml yielded an AUC of 0.936 (95 % CI 0.817-0.988) in identifying patients with poor functional outcome, at the sensitivity and specificity of 95.7 and 80.0 %, respectively. GFAP test is a promising technique for diagnosis of ICH from IS and prediction of short-term functional outcomes.
authors.affiliation: Department of Neurology, Wuhan Central Hospital, 26 Shengli Road, Wuhan City, 430014, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Neurology, Wuhan Central Hospital, 26 Shengli Road, Wuhan City, 430014, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurology, Wuhan Central Hospital, 26 Shengli Road, Wuhan City, 430014, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Wuhan Central Hospital, 26 Shengli Road, Wuhan City, 430014, People's Republic of China. xwp881019@sina.com.
authors.forename: Wuping
authors.initials: W
authors.lastname: Xu
mesh_terms: D000368:Aged; D019540:Area Under Curve; D044466:Asian People; D015415:Biomarkers / Q000097:blood; D002545:Brain Ischemia / Q000097:blood / Q000175:diagnosis / Q000628:therapy; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000175:diagnosis / Q000628:therapy; D002681:China; D003937:Diagnosis, Differential; D004185:Disability Evaluation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000097:blood*; D006801:Humans; D008297:Male; D011379:Prognosis; D012372:ROC Curve; D020127:Recovery of Function / Q000502:physiology; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D020521:Stroke / Q000097:blood / Q000175:diagnosis / Q000628:therapy; D013997:Time Factors
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein
keywords: Acute stroke; Biomarker; Glial fibrillary acidic protein; Intracerebral hemorrhage