title: Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTXVv as a potential vaccine for Vibrio vulnificus.
abstract: Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus. However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPDVv (C3725S), of the well-conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats-in-toxin (MARTXVv /RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPDVv (C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.
authors.affiliation: Department of Microbiology and Immunology.
authors.forename: Tae Hee
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Immunology.|Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Jeonbuk, 561-756, Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Chung
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000097:blood; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D057056:Cysteine Proteases / Q000235:genetics / Q000276:immunology*; D005260:Female; D007116:Immunization, Passive; D051379:Mice; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D041261:Vibrio vulnificus / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*
chemical_list: D000992:Antitoxins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic; D057056:Cysteine Proteases
keywords: RtxA1/MARTXVv; Vibrio vulnificus; cysteine protease domain; protective immunity