title: Effects of thermo-chemical pretreatment plus microbial fermentation and enzymatic hydrolysis on saccharification and lignocellulose degradation of corn straw.
abstract: In order to increase corn straw degradation, the straw was kept in the combined solution of 15% (w/w) lime supernatant and 2% (w/w) sodium hydroxide with liquid-to-solid ratio of 13:1 (mL/g) at 83.92°C for 6h; and then added with 3% (v/v) H2O2 for reaction at 50°C for 2h; finally cellulase (32.3 FPU/g dry matter) and xylanase (550 U/g dry matter) was added to keep at 50°C for 48 h. The maximal reducing sugars yield (348.77 mg/g) was increased by 126.42% (P<0.05), and the degradation rates of cellulose, hemicellulose and lignin in pretreated corn straw with enzymatic hydrolysis were increased by 40.08%, 45.71% and 52.01%, compared with the native corn straw with enzymatic hydrolysis (P<0.05). The following study indicated that the combined microbial fermentation and enzymatic hydrolysis could further increase straw degradation and reducing sugar yield (442.85 mg/g, P<0.05).
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: qqy1964@126.com.
authors.forename: Qingqiang
authors.initials: Q
authors.lastname: Yin
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Erzhu
authors.initials: E
authors.lastname: Wang
authors.affiliation: Henan Delin Biological Product Co. Ltd., Xinxiang 453000, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Andong
authors.initials: A
authors.lastname: Song
authors.affiliation: Henan Engineering and Technology Research Center of Feed Microbes, Zhoukou 466000, China.
authors.forename: Fushan
authors.initials: F
authors.lastname: Lu
mesh_terms: D000704:Analysis of Variance; D001419:Bacteria / Q000378:metabolism*; D017610:Calcium Compounds / Q000494:pharmacology; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013696:Temperature; D014866:Waste Products; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D017610:Calcium Compounds; D010087:Oxides; D014866:Waste Products; C036909:lignocellulose; D012972:Sodium Hydroxide; D002482:Cellulose; D008031:Lignin; D006861:Hydrogen Peroxide; C016538:lime; D002480:Cellulase
keywords: Corn straw; Enzymatic hydrolysis; Microbial fermentation; Saccharification; Thermo-chemical pretreatment