title: On-line concentration and pressurized capillary electrochromatography analysis of five β-agonists in human urine using a methacrylate monolithic column.
abstract: A pressurized capillary electrochromatography (pCEC) method combined with an online concentration was developed for the separation and determination of five β2-blockers (terbutaline, salbutamol, formoterol, procaterol and salmeterol) in human urine. A stearyl methacrylate-based monolithic column was prepared as the separation column. The various separation parameters including acetontrile concentration, applied voltage, pH and concentration of the running buffer were investigated. On-line concentration methods in combination of the chromatographic zone-sharpening effect and field-enhanced sample-stacking effect were utilized to improve detection sensitivity. The on-line concentration parameters including injection voltage, injection time, as well as sample matrix were systematically studied. Compared with the conventional sample injection, the on-line concentration technique appeared to improve the corresponding sensitivities 20∽50-fold. Furthermore, good precision was obtained with relative standard deviations (RSDs) for retention times within 1.09% and for peak areas less than 3.55% (n = 5). The established method was successfully applied to the determination of above-mentioned β2-agonists in urine samples. The recoveries of spiked urine samples were between 85.0 and 113.3% with RSDs less than 5.39%.
authors.affiliation: Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province Department of Chemistry, Shangrao Normal University, Jiangxi, P. R., China.|Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
authors.forename: Zongbao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province Department of Chemistry, Shangrao Normal University, Jiangxi, P. R., China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
keywords: Monolithic column; On-line concentration; Pressurized capillary electrochromatography; β2-Agonists