title: Apoptosis-inducing Factor, Mitochondrion-associated 2, Regulates Klf1 in a Mouse Erythroleukemia Cell Line.
abstract: OBJECTIVE
  Apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), is a DNA-binding oxoreductase protein that promotes apoptosis. To assess its potential role in erythropoiesis we analyzed the effects of Aifm2 loss-of-function in the murine erythroleukemia line (MEL).
  
  
  METHODS
  MEL cells were transfected with siRNA targeting Aifm2 for 24 h and evaluated by cell counting, flow cytometry with annexin V and PI staining and gene expression analysis.
  
  
  RESULTS
  Aifm2 knockdown did not affect the apoptotic status of MEL cells. However, Aifm2 knockdown significantly increased expression of the erythropoietic transcription factor Klf1 (2.9±0.2-fold, p<0.05) and decreased α- and β-globin expression (0.6±0.2-fold, p<0.05 and 0.5±0.2-fold, p<0.01).
  
  
  CONCLUSIONS
  Aifm2 may function in differentiation of erythroid MEL cells in vitro.
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kojima
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kasem
authors.initials: K
authors.lastname: Kulkeaw
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Senji
authors.initials: S
authors.lastname: Shirasawa
authors.affiliation: Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan ds-mons@yb3.so-net.ne.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D004920:Erythropoiesis / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D010088:Oxidoreductases / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D055542:alpha-Globins / Q000096:biosynthesis; D055544:beta-Globins / Q000096:biosynthesis
chemical_list: D051017:Apoptosis Regulatory Proteins; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; D055542:alpha-Globins; D055544:beta-Globins; C080774:erythroid Kruppel-like factor; C530020:ferroptosis suppressor protein 1, mouse; D010088:Oxidoreductases
keywords: apoptosis-inducing factor; erythropoiesis; mitochondrion-associated 2 (Aifm2); murine erythroleukemia cells