title: Ovalbumin Delivery by Guanidine-Terminated Dendrimers Bearing an Amyloid-Promoting Peptide via Nanoparticle Formulation.
abstract: Development of protein delivery systems is important for biomedical applications such as immunotherapy. Ovalbumin (OVA) is a major component of egg whites, and is a possible cause of egg allergy. In this study, OVA was used as a model protein to develop a delivery system using guanidine-terminated dendrimers (Gdn-den) bearing an amyloid-promoting peptide derived from the helix B (hB) region of OVA (hB-Gdn-den). OVA nanoparticles (NPs) were prepared by heat treatment of OVA/hB-Gdn-den mixtures. The NP size and the surface charge were controlled by adjusting the ratio of hB-Gdn-den to OVA. The NPs were around 200 nm in diameter and stably dispersed, and their encapsulation efficiency for OVA was more than 80%. Although OVA NPs were also prepared using Gdn-den, the NPs aggregated readily. Complexation with hB-Gdn-den induced conformational changes in the OVA, and the hB peptide promoted digestion of OVA. These suggest that the hB peptide of the Gdn-den works as a possible anchor to OVA. The positively charged OVA NPs effectively associated with RAW264 cells. Thus, the amyloid-promoting Gdn-den, when mixed with OVA at a suitable molar ratio to form NPs, could act as a carrier for delivery of antigen proteins to immune cells.
authors.affiliation: †Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Kojima
authors.affiliation: ‡Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Gosyokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Kameyama
authors.affiliation: ‡Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Gosyokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Yamada
authors.affiliation: ‡Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Gosyokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ichikawa
authors.affiliation: ‡Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Gosyokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Waku
authors.forename: Akihiro
authors.initials: A
authors.lastname: Handa
authors.affiliation: ‡Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Gosyokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D050091:Dendrimers / Q000737:chemistry*; D016503:Drug Delivery Systems; D019791:Guanidine / Q000737:chemistry*; D008264:Macrophages; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000008:administration & dosage* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry*
chemical_list: D000682:Amyloid; D050091:Dendrimers; D010446:Peptide Fragments; D010047:Ovalbumin; D019791:Guanidine