title: Cytoplasmic 5-Lipoxygenase Staining Is a Highly Sensitive Marker of Human Tumors of the Choroid Plexus.
abstract: OBJECTIVE
  To determine the immunoreactivity status of 5-lipoxygenase (5-LO) in normal tissues, in tumors of the human choroid plexus, and in other brain tumors.
  
  
  METHODS
  In total, 135 cases of various types of brain tumors were selected. Tissue samples were immunostained with a rabbit polyclonal anti-5-LO antibody.
  
  
  RESULTS
  Nuclear reactivity was observed in most brain tumors, with most of the positive tumor cells exhibiting low-level reactivity. Cytoplasmic strong immunoreactivity for 5-LO (2+ or 3+) was only observed in 8.8% of astrocytic tumors, 0% of oligodendrogliomatous tumors, 5.6% of ependymal tumors, 0% of embryonal tumors, 3.1% of meningeal tumors, and 0% of metastatic lung adenocarcinomas. In contrast, cytoplasmic immunoreactivity for 5-LO was detected in all 27 cases of choroid plexus tumors. Twenty-five cases showed strong and diffuse cytoplasmic immunoreactivity.
  
  
  CONCLUSIONS
  Our findings indicate that cytoplasmic 5-LO immunoreactivity is highly characteristic of human choroid plexus tumors but not other central nervous system tumor types. Cytoplasmic staining for 5-LO may prove to be a useful immunoreactive marker in the diagnosis of choroid plexus tumors.
authors.affiliation: From the Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China;
authors.forename: Wang
authors.initials: W
authors.lastname: Xingfu
authors.affiliation: Department of Endocrinology, Fujian Province Governmental Hospital, Fuzhou, China and.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lifeng
authors.affiliation: From the Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China;
authors.forename: Chen
authors.initials: C
authors.lastname: Yupeng
authors.affiliation: From the Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China;
authors.forename: Liu
authors.initials: L
authors.lastname: Xueyong
authors.affiliation: Department of Pathology, Fuzhou General Hospital of Nanjing Command, PLA, Fuzhou, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Pathology, Fuzhou General Hospital of Nanjing Command, PLA, Fuzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yinghao
authors.affiliation: From the Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China;
authors.forename: Cai
authors.initials: C
authors.lastname: Suqin
authors.affiliation: From the Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China;
authors.forename: Wang
authors.initials: W
authors.lastname: Mi
authors.affiliation: From the Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China; zhgshg@126.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Sheng
mesh_terms: D001094:Arachidonate 5-Lipoxygenase / Q000032:analysis* / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000032:analysis*; D016545:Choroid Plexus Neoplasms / Q000175:diagnosis* / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D014408:Biomarkers, Tumor; D001094:Arachidonate 5-Lipoxygenase
keywords: 5-LO; 5-Lipoxygenase; Brain tumor; Choroid plexus tumor; Immunohistochemistry