title: [Cloning of alternative isoforms of the catalytic subunit of the human thelomerase (htert)].
abstract: Most human tumors, including cervical cancer, are characterized by telomerase activation (cell proliferation activation enzyme). Such activation is implemented in the elongation of the terminal segments (telomeres) of the telomerase chromosome. The gene of the enzyme is RNA-encoded, the RNA in tumors being observed in a few isoforms. The hTERT RNA role in cell activation and control was simulated using cervical cancer, as well as its pretumoral states (CIN), as a model object. The goal of this work was to clone of the human hTERT isoforms (normal, α-, β-, and α+β-splice-variants). The genetic constructions containing normal hTERT sequence, α- and β-deletion variants based on the lentivirus vector pR780 were obtained. The α- and β-deletion variants were not obtained in this variant because of methodological problems. In further research, we plan to implement splice-variants of hTERT in eukaryotic human cells.
authors.forename: V G
authors.initials: VG
authors.lastname: Khomenkov
authors.forename: M Yu
authors.initials: MY
authors.lastname: Skoblov
authors.forename: L I
authors.initials: LI
authors.lastname: Korolenkova
authors.forename: F L
authors.initials: FL
authors.lastname: Kiselev
mesh_terms: D017398:Alternative Splicing; D020134:Catalytic Domain; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D021122:Protein Subunits; D012333:RNA, Messenger; D017384:Sequence Deletion; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics
chemical_list: D021122:Protein Subunits; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase