title: Proteomic analysis of early-stage embryos: implications for egg quality in hapuku (Polyprion oxygeneios).
abstract: In order to develop biomarkers that may help predict the egg quality of captive hapuku (Polyprion oxygeneios) and provide potential avenues for its manipulation, the present study (1) sequenced the proteome of early-stage embryos using isobaric tag for relative and absolute quantification analysis, and (2) aimed to establish the predictive value of the abundance of identified proteins with regard to egg quality through regression analysis. Egg quality was determined for eight different egg batches by blastomere symmetry scores. In total, 121 proteins were identified and assigned to one of nine major groups according to their function/pathway. A mixed-effects model analysis revealed a decrease in relative protein abundance that correlated with (decreasing) egg quality in one major group (heat-shock proteins). No differences were found in the other protein groups. Linear regression analysis, performed for each identified protein separately, revealed seven proteins that showed a significant decrease in relative abundance with reduced blastomere symmetry: two correlates that have been named in other studies (vitellogenin, heat-shock protein-70) and a further five new candidate proteins (78 kDa glucose-regulated protein, elongation factor-2, GTP-binding nuclear protein Ran, iduronate 2-sulfatase and 6-phosphogluconate dehydrogenase). Notwithstanding issues associated with multiple statistical testing, we conclude that these proteins, and especially iduronate 2-sulfatase and the generic heat-shock protein group, could serve as biomarkers of egg quality in hapuku.
authors.affiliation: Department of Zoology, University of Otago, PO Box 56, 340 Great King St., Dunedin, 9016, New Zealand.|Bream Bay Aquaculture Park, NIWA, PO Box 147, Ruakaka, 0151, New Zealand.|Arava Research and Development Station, Hatzeva, Israel.
authors.forename: Yair Y
authors.initials: YY
authors.lastname: Kohn
authors.affiliation: Bream Bay Aquaculture Park, NIWA, PO Box 147, Ruakaka, 0151, New Zealand.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Symonds
authors.affiliation: Centre for Protein Research, University of Otago, PO Box 56, Dunedin, 9054, New Zealand.
authors.forename: Torsten
authors.initials: T
authors.lastname: Kleffmann
authors.affiliation: Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Lagisz
authors.affiliation: Department of Zoology, University of Otago, PO Box 56, 340 Great King St., Dunedin, 9016, New Zealand. mark.lokman@otago.ac.nz.
authors.forename: P Mark
authors.initials: PM
authors.lastname: Lokman
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001757:Blastomeres / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology*; D047108:Embryonic Development; D006360:Heat-Shock Proteins / Q000378:metabolism; D010063:Ovum / Q000502:physiology*; D010473:Perciformes / Q000196:embryology*; D020543:Proteome
chemical_list: D015415:Biomarkers; D006360:Heat-Shock Proteins; D020543:Proteome
keywords: Biomarkers; Egg quality; Hapuku; Polyprion oxygeneios; Proteomics; iTRAQ