title: The epitope analysis of an antibody specifically against Vibrio cholerae O1 Ogawa by phage library study.
abstract: To prevent epidemic and pandemic cholera disease, an indispensible approach is to develop cholera vaccines based on comprehensive epitope information of this pathogen. This study aimed to utilize our previously raised monoclonal antibody IXiao3G6, which can recognize an epitope in lipopolysaccharide (LPS) sites of Ogawa, to identify mimetic peptides, which may represent Ogawa LPS's epitope information. A phage display library screening using IXiao3G6 antibody resulted in identification of a mimic peptide (MP) with high avidity. A recombinant protein, containing one cholera toxin subunit B (CTB) and two MP repeats (CTB-(MP)2), was subsequently constructed and investigated for its immunological characteristics. The findings collectively demonstrated that the MP presenting phages and CTB-(MP)2 recombinant protein were both capable of inhibiting the interaction between IXiao3G6 and Ogawa/Ogawa LPS specifically in a dose-dependent manner.
authors.affiliation: Clinical Laboratory, Shandong Jiaotong Hospital, Jinan, Shandong, China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Chongqing Medical University, Chongqing, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Clinical Laboratory, Shandong Jiaotong Hospital, Jinan, Shandong, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Clinical Laboratory, Shandong Jiaotong Hospital, Jinan, Shandong, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Artron BioResearch Inc., Burnaby, British Columbia, Canada.
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.affiliation: Key Laboratory of Diagnostic Medicine, Chongqing Medical University, Chongqing, China.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Tu
authors.affiliation: Artron BioResearch Inc., Burnaby, British Columbia, Canada.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Chongqing Medical University, Chongqing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Diagnostic Medicine, Chongqing Medical University, Chongqing, China.. Electronic address: zhangjunlg@hotmail.com.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Yu
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D019151:Peptide Library; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D041241:Vibrio cholerae O1 / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D019151:Peptide Library; D011994:Recombinant Proteins
keywords: Cholera; Lipopolysaccharide; Mimic peptide; Monoclonal antibody; Ogawa; Phage display library