title: Advances in de novo strain design using integrated systems and synthetic biology tools.
abstract: Recent efforts in expanding the range of biofuel and biorenewable molecules using microbial production hosts have focused on the introduction of non-native pathways in model organisms and the bio-prospecting of non-model organisms with desirable features. Current challenges lie in the assembly and coordinated expression of the (non-)native pathways and the elimination of competing pathways and undesirable regulation. Several systems and synthetic biology approaches providing contrasting top-down and bottom-up strategies, respectively, have been developed. In this review, we discuss recent advances in both in silico and experimental approaches for metabolic pathway design and engineering, with a critical assessment of their merits and remaining challenges.
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Chiam Yu
authors.initials: CY
authors.lastname: Ng
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Khodayari
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Anupam
authors.initials: A
authors.lastname: Chowdhury
authors.affiliation: Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA. Electronic address: costas@psu.edu.
authors.forename: Costas D
authors.initials: CD
authors.lastname: Maranas
mesh_terms: D000818:Animals; D003198:Computer Simulation; D023281:Genomics / Q000379:methods*; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D058615:Synthetic Biology / Q000379:methods*; D049490:Systems Biology / Q000379:methods