title: Permeapad™ for investigation of passive drug permeability: The effect of surfactants, co-solvents and simulated intestinal fluids (FaSSIF and FeSSIF).
abstract: The aim of the present work was to investigate the potential of the new and innovative artificial barrier, Permeapad™, when exposed to surfactants and co-solvents, often employed for poorly water soluble compounds. The barrier was in addition also exposed to fasted and fed state simulated intestinal fluids versions 1 and 2 (FaSSIF and FeSSIF), all of which the Permeapad™ barrier was compatible with based upon relative comparison of the permeability of the hydrophilic marker calcein in phosphate buffer. The new barrier therefore holds a huge potential due to its functional stability and robustness. It can be used as a standard tool to investigate permeability of drugs in the presence of different surfactants and co-solvents, from DMSO stock solutions at even high concentrations and for the evaluation of permeability in the presence of biomimetic media (BMM).
authors.affiliation: Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK-5230 Odense, Denmark.
authors.forename: Hanady Ajine
authors.initials: HA
authors.lastname: Bibi
authors.affiliation: Drug Transport and Delivery Research Group, Department of Pharmacy, UiT-The Arctic University of Norway, Universitetsveien 57, 9037 Tromsø, Norway.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: di Cagno
authors.affiliation: Biologics and Pharmaceutical Science, H. Lundbeck A/S, Ottiliavej 9, DK-2500 Valby, Denmark.
authors.forename: Rene
authors.initials: R
authors.lastname: Holm
authors.affiliation: Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK-5230 Odense, Denmark. Electronic address: annette.bauer@sdu.dk.
authors.forename: Annette
authors.initials: A
authors.lastname: Bauer-Brandl
mesh_terms: D032701:Biomimetics; D005452:Fluoresceins / Q000378:metabolism; D007408:Intestinal Absorption; D007419:Intestinal Secretions / Q000378:metabolism*; D010539:Permeability; D012997:Solvents / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D005452:Fluoresceins; D012997:Solvents; D013501:Surface-Active Agents; C007740:fluorexon
keywords: Apparent permeability; Caffeine (PubChem CID: 2519); Calcein (PubChem CID: 65079); Co-solvents; Dimethylsulphoxide (DMSO) (PubChem CID: 679); FaSSIF; FeSSIF; MacrogolG R (PubChem CID: 81307); Permeapad™; Polysorbate 80 (PubChem CID: 5284448); Sodiumdocecylsulphate (SDS) (PubChem CID: 8778); Surfactants; Triton-X (PubChem CID: 5590)