title: Impact of daily supplementation of Spirulina platensis on the immune system of naïve HIV-1 patients in Cameroon: a 12-months single blind, randomized, multicenter trial.
abstract: BACKGROUND
  Micronutrient deficiencies occur early in Human Immunodeficiency Virus (HIV) infections they have reverse effects on the nutritional status. The diet supplementation with a natural nutraceutical rich in proteins and micronutrient like Spirulina platensis, may be effective and efficient in delaying HIV disease progression by frequently reported improvement in immune response.
  
  
  METHODS
  A prospective single-blind, randomized, multicenter study conducted on 320 HIV-1 ARV-naïve participants for 12 months. Participants received either S. platensis supplementation and standard care or standard care and local balanced diet without S. platenis. Selected hematological and biochemical as well as CD4 count cells, viral load copies were assessed at three separate times.
  
  
  RESULTS
  Among the 169 ART-naïve participants enrolled in the study, the female was mostly represented (67.1%). The significant increase of CD4 count cells (596.32-614.92 cells count) and significant decrease of viral load levels (74.7 × 10(3)-30.87 × 10(3) copies/mL) of the patients who received a supplementation of S. platensis was found after 6 months of treatment. Haemoglobin level was also significantly higher in the same group while the fasting blood glucose concentration decreased after 12 months compared to control.
  
  
  CONCLUSIONS
  A daily supplementation with S. platensis to diet combined with a reasonable balanced diet has significantly increased the CD4 cells and reduced the viral load after 6 months. Further studies are recommended among a large specific group of people infected by the HIV in order to investigate the mechanisms involved on the effect of S. platensis on immune system.
authors.affiliation: National Institute of Agro-Industrial Sciences, University of Ngaoundere, POBOX 455, Ngaoundere, Cameroun. m_matipde@yahoo.de.
authors.forename: Marthe-Elise
authors.initials: ME
authors.lastname: Ngo-Matip
authors.affiliation: Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, POBOX 1634, Yaounde, Cameroon. apieme@yahoo.fr.
authors.forename: Constant Anatole
authors.initials: CA
authors.lastname: Pieme
authors.affiliation: Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, POBOX 1634, Yaounde, Cameroon. azabji@gmail.com.
authors.forename: Marcel
authors.initials: M
authors.lastname: Azabji-Kenfack
authors.affiliation: National Institute of Agro-Industrial Sciences, University of Ngaoundere, POBOX 455, Ngaoundere, Cameroun. mouket2006@yahoo.fr.
authors.forename: Bruno Moukette
authors.initials: BM
authors.lastname: Moukette
authors.affiliation: Spirulina Producer's Federation of France, Hyères, France. spiruvie@free.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Korosky
authors.affiliation: Ecole de formation Agricole de Hyères, Hyères, France. philippe.stafanini@educagri.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Stefanini
authors.affiliation: Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, POBOX 1634, Yaounde, Cameroon. jngogang@yahoo.fr.
authors.forename: Jeanne Yonkeu
authors.initials: JY
authors.lastname: Ngogang
authors.affiliation: National Institute of Agro-Industrial Sciences, University of Ngaoundere, POBOX 455, Ngaoundere, Cameroun. cmbofung@yahoo.com.
authors.forename: Carl Moses
authors.initials: CM
authors.lastname: Mbofung
mesh_terms: D000328:Adult; D015992:Body Mass Index; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002163:Cameroon; D004032:Diet; D019587:Dietary Supplements; D005260:Female; D005500:Follow-Up Studies; D015658:HIV Infections / Q000097:blood / Q000628:therapy*; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D018977:Micronutrients / Q000008:administration & dosage / Q000097:blood / Q000172:deficiency; D009752:Nutritional Status; D011446:Prospective Studies; D016037:Single-Blind Method; D053545:Spirulina / Q000378:metabolism*; D019562:Viral Load
chemical_list: D018977:Micronutrients