title: Supramolecular Peptide/Surface Assembly for Monitoring Proteinase Activity and Cancer Diagnosis.
abstract: Matrix metalloproteinases (MMP) are a family of proteolytic enzymes, the expression of which in a key step of tumor progression has recently been better defined. The overexpression of one or more MMPs is thus common among malignant tumors. It may characterize tumor progression and help predict its response to chemotherapy. Consequently, the development of a device for measuring MMP activities is an important challenge for diagnosis and prognosis. In this study, we describe an innovative supramolecular peptide/surface assembly for screening MMP activities. This sensor was used to discriminate various MMP activities and to distinguish between invasive and noninvasive cancerous cell suspensions. Our results confirm the proof-of-concept of a powerful tool for the determination of the tumor aggressiveness and a technical building block for future development of MMP lab-on-chip devices.
authors.affiliation: †Pharmacochimie, FRE3396 CNRS, University of Bordeaux, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France.|∥Laboratory of Innovation in Surface Chemistry and Nanosciences, NIMBE UMR CNRS 3685, CEA Saclay, DSM/IRAMIS/NIMBE/LICSEN, F-91191, Gif-sur-Yvette Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Soum
authors.affiliation: †Pharmacochimie, FRE3396 CNRS, University of Bordeaux, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rubio-Albenque
authors.affiliation: ‡Institut des Technologies Avancées en sciences du Vivant (ITAV), CNRS UMS 3039, Centre Pierre Potier, Oncopôle, 1 place Pierre Potier, BP 50624, F-31106 Toulouse Cedex 1, France.|§LSPCMIB, UMR-CNRS 5068, Université Paul Sabatier-Toulouse III, 118, route de Narbonne, F-31062 Toulouse Cedex 9, France.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Fery-Forgues
authors.forename: Gérard
authors.initials: G
authors.lastname: Déléris
authors.affiliation: †Pharmacochimie, FRE3396 CNRS, University of Bordeaux, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France.
authors.forename: Mohamed-Anis
authors.initials: MA
authors.lastname: Alouini
authors.affiliation: ∥Laboratory of Innovation in Surface Chemistry and Nanosciences, NIMBE UMR CNRS 3685, CEA Saclay, DSM/IRAMIS/NIMBE/LICSEN, F-91191, Gif-sur-Yvette Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Berthelot
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D056656:Lab-On-A-Chip Devices; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D012720:Severity of Illness Index; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D047392:beta-Cyclodextrins; D020782:Matrix Metalloproteinases; C031215:betadex
keywords: cancer; cancerous cells; diagnosis; matrix metalloproteinase; regenerative surface; supramolecular assembly