title: Identification of Microbial Metabolites Elevating Vitamin Contents in Barley Seeds.
abstract: The current investigation analyzes metabolites of Acetobacter aceti to explore chemical compounds responsible for the induction of vitamins in barley seeds. A bioactivity guided assay of bacterial extracts and chromatographic analyses of barley produce revealed 13 chemical compounds, which were subjected to principal component analysis (PCA). PCA determined four chemical compounds (i.e., quinolinic acid, pyridoxic acid, p-aminobenzoate, and α-oxobutanoic acid) highly associated with increased quantities of vitamins. Further experimentations confirmed that quinolinic acid and p-aminobenzoate were the most efficient vitamin inducers. The results indicated chloroform/ethanol (4:1) as the best solvent system for the extraction of active compounds from crude metabolites of A. aceti. Significant quantities of mevalonic acid were detected in the extracted fraction, indicating the possible induction of the isoprenoid pathway. Altogether, the current investigation broadens the frontiers in plant-microbe interaction.
authors.affiliation: †College of Earth and Environmental Sciences and ‡Institute of Agricultural Sciences, University of the Punjab, Lahore-54590, Pakistan.
authors.forename: Anam
authors.initials: A
authors.lastname: Yousaf
authors.affiliation: †College of Earth and Environmental Sciences and ‡Institute of Agricultural Sciences, University of the Punjab, Lahore-54590, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Qadir
authors.affiliation: †College of Earth and Environmental Sciences and ‡Institute of Agricultural Sciences, University of the Punjab, Lahore-54590, Pakistan.
authors.forename: Tehmina
authors.initials: T
authors.lastname: Anjum
authors.affiliation: †College of Earth and Environmental Sciences and ‡Institute of Agricultural Sciences, University of the Punjab, Lahore-54590, Pakistan.
authors.forename: Aqeel
authors.initials: A
authors.lastname: Ahmad
mesh_terms: D000091:Acetobacter / Q000737:chemistry / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry / Q000382:microbiology*; D012639:Seeds / Q000737:chemistry* / Q000382:microbiology; D014815:Vitamins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014815:Vitamins
keywords: bioactivity guided assay; chromatographic analysis; fractionation; precursor; transition state