title: Ca(2+)/Calmodulin-Dependent Protein Kinase IV Promotes Interplay of Proteins in Chromatoid Body of Male Germ Cells.
abstract: The chromatoid body is a granule-like structure of male germ cells, containing many proteins and RNAs, and is important for spermatogenesis. However, the molecular mechanisms for the formation and function of the chromatoid body are still elusive. Here, we report that Ca(2+)/calmodulin-dependent protein kinase IV (CaMKIV) accumulates in the chromatoid body by immunofluorescence staining, indicating that CaMKIV is a new component of the chromatoid body. Furthermore, we find that CaMKIV can interplay with the other components of the chromatoid body by immunoprecipitation: mouse VASA homologue (MVH), mouse homologue of PIWI, PIWIL1 (MIWI), and kinesin KIF17b. Importantly, interplay between KIF17b and MVH or MIWI can be potentially regulated by CaMKIV. These results imply that CaMKIV plays a role in maintenance the structure of chromatoid body by regulating the associations of proteins in it.
authors.affiliation: International Peace Maternity &Child Health Hospital, Institute of Embryo-Fetal Original Adult Disease Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Guishuan
authors.initials: G
authors.lastname: Wang
authors.affiliation: International Peace Maternity &Child Health Hospital, Institute of Embryo-Fetal Original Adult Disease Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: International Peace Maternity &Child Health Hospital, Institute of Embryo-Fetal Original Adult Disease Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: International Peace Maternity &Child Health Hospital, Institute of Embryo-Fetal Original Adult Disease Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: International Peace Maternity &Child Health Hospital, Institute of Embryo-Fetal Original Adult Disease Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Hefeng
authors.initials: H
authors.lastname: Huang
authors.affiliation: International Peace Maternity &Child Health Hospital, Institute of Embryo-Fetal Original Adult Disease Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4 / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*
chemical_list: D011506:Proteins; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4