title: A reliable method for the selection and confirmation of transconjugants of plant growth-promoting bacteria especially plant-associated Burkholderia spp.
abstract: Selectable markers, e.g., antibiotic resistance, for conjugation experiments are not always effective for slow-growing plant growth promoting bacteria such as Burkholderia. We used PCAT medium containing Congo Red for selecting Burkholderia transconjugants. This method allows for the reliable selection of transconjugants of these novel plant growth-promoting bacteria.
authors.affiliation: National Institute for Biotechnology & Genetic Engineering, Faisalabad, Pakistan; Government College University Faisalabad, Allama Iqbal Road, Faisalabad, Pakistan.
authors.forename: Mohsin
authors.initials: M
authors.lastname: Tariq
authors.affiliation: Department of Biology, Loyola Marymount University, Los Angeles, CA, USA.
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Lum
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
authors.forename: Allan W
authors.initials: AW
authors.lastname: Chong
authors.affiliation: Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, USA.
authors.forename: Anjana B
authors.initials: AB
authors.lastname: Amirapu
authors.affiliation: National Institute for Biotechnology & Genetic Engineering, Faisalabad, Pakistan.
authors.forename: Sohail
authors.initials: S
authors.lastname: Hameed
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA. Electronic address: ahirsch@ucla.edu.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Hirsch
mesh_terms: D019117:Burkholderia / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D058948:Cell Tracking; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D010944:Plants / Q000382:microbiology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: Congo Red; Conjugation; PCAT; PGPB; Transconjugants