title: Rabbit Hemorrhagic Disease Virus Variant Recombinant VP60 Protein Induces Protective Immunogenicity.
abstract: Rabbit hemorrhagic disease virus (RHDV) is highly contagious and often causes fatal disease that affects both wild and domestic rabbits of the species Oryctolagus cuniculus. A highly pathogenic RHDV variant (RHDVa) has been circulation in the Korean rabbit population since 2007 and has a devastating effect on the rabbit industry in Korea. A highly pathogenic RHDVa was isolated from naturally infected rabbits, and the gene encoding the VP60 protein was cloned into a baculovirus transfer vector and expressed in insect cells. The hemagglutination titer of the Sf-9 cell lysate infected with recombinant VP60 baculovirus was 131,072 units/50 μl and of the supernatant 4,096 units/50 μl. Guinea pigs immunized twice intramuscularly with a trial inactivated RHDVa vaccine containing recombinant VP60 contained 2,152 hemagglutination inhibition (HI) geometric mean titers. The 8-week-old white rabbits inoculated with one vaccine dose were challenged with a lethal RHDVa 21 days later and showed 100% survival rates. The recombinant VP60 protein expressed in a baculovirus system induced high HI titers in guinea pigs and rendered complete protection, which led to the development of a novel inactivated RHDVa vaccine.
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors.forename: Dong-Kun
authors.initials: DK
authors.lastname: Yang
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors.forename: Ha-Hyun
authors.initials: HH
authors.lastname: Kim
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Nah
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae / Q000235:genetics; D017250:Caliciviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D003001:Cloning, Molecular; D015870:Gene Expression; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D007723:Korea; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D061987:Sf9 Cells; D018411:Spodoptera; D016019:Survival Analysis; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus
keywords: Rabbit hemorrhagic disease virus; recombinant protein; vaccine