title: Severe hypoxia and malnutrition collectively contribute to scar fibroblast inhibition and cell apoptosis.
abstract: This study aims to investigate whether severe hypoxia and malnutrition in scar tissue play key roles to induce hypertrophic scar regression. And scar-derived fibroblasts were treated with moderate/severe hypoxia and malnutrition to model condition of proliferative and regressive scar (5%O2 +5%FCS and 0.5%O2  + 0.5%FCS), and normoxia with well nutrition as control (10%O2  + 10%FCS). Our results demonstrated that severe hypoxia and malnutrition resulted in significantly reduced cell viability and collagen production, as well as HIF-1, VEGF, TGF-β1, and Bcl-2 protein expression when compared with control, and cell apoptosis occurred. Therefore, the severe hypoxia and malnutrition in scar tissue contribute to fibroblast inhibition and cell apoptosis, which is correlated with scar regression.
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia.
authors.forename: Emily C
authors.initials: EC
authors.lastname: Lynam
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia. Tissue Organ Bank & Tissue Engineering Centre, General Hospital of Ningxia Medical University, Ningxia, China
authors.forename: Yan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Dawson
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia.
authors.forename: Jacqui
authors.initials: J
authors.lastname: Mcgovern
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia.
authors.forename: Zee
authors.initials: Z
authors.lastname: Upton
authors.affiliation: Burn Centre, RuiJin Hospital, Jiao Tong University Medical School, Shanghai, China
authors.forename: XiQiao
authors.initials: X
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002470:Cell Survival; D002478:Cells, Cultured; D002921:Cicatrix / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D000860:Hypoxia / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D020287:In Situ Nick-End Labeling; D044342:Malnutrition / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing