title: Water Extract of Mentha x villosa: Phenolic Fingerprint and Effect on Ischemia-Reperfusion Injury.
abstract: Qualitative analysis of the water extract of Mentha x villosa Huds. leaves was performed by liquid chromatography mass spectrometry (LC-MS/MS) and quantitative analysis was made by reverse-phase liquid chromatography coupled with photodiode array detection (LC-DAD). Sixteen phenolic compounds were identified and quantified consisting of 8 phenolic acids/derivatives and 8 flavonoid glycosides (quinic acid, chlorogenic acid, coumaroyl-hexoside, caffeic acid, coumaroylquinic acid, lithospermic acid, rosmarinic acid, salvianolic acid A, luteolin-7-O-glucuronide, luteolin-7-O-glucoside, luteolin-7-O-rutinoside, eriodictyol-7-O-rutinoside, apigenin-7-O-glucuronide, kaempferol-3-O-glucuronide, chrysoeriol-7-O-rutinoside, and hesperetin-7-O-rutinoside). Luteolin-7- O-rutinoside (25.6 ± 0.7 mg/g dry extract) and rosmarinic acid (17.9 ± 0.4 mg/g dry extract) were the most abundant. High antioxidant activity of this phenolic-rich water extract was confirmed in vitro by DPPH and ABTS tests and ex vivo in the ischemia-reperfusion injured rat superior mesenteric artery. Thus, the water extract of M. x villosa leaves seems to be a promising agent in prevention of tissue injury caused by oxidative stress.
authors.forename: Silvia
authors.initials: S
authors.lastname: Fialovaa
authors.forename: Lucia
authors.initials: L
authors.lastname: Veizerova
authors.forename: Viera
authors.initials: V
authors.lastname: Nosalova
authors.forename: Katarina
authors.initials: K
authors.lastname: Drabikova
authors.forename: Daniela
authors.initials: D
authors.lastname: Tekelova
authors.forename: Daniel
authors.initials: D
authors.lastname: Grancai
authors.forename: Ruzena
authors.initials: R
authors.lastname: Sotnikova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D027541:Mentha / Q000737:chemistry*; D010636:Phenols / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts