title: Grhl2 is required in nonneural tissues for neural progenitor survival and forebrain development.
abstract: Grainyhead-like genes are part of a highly conserved gene family that play a number of roles in ectoderm development and maintenance in mammals. Here we identify a novel allele of Grhl2, cleft-face 3 (clft3), in a mouse line recovered from an ENU mutagenesis screen for organogenesis defects. Homozygous clft3 mutants have a number of phenotypes in common with other alleles of Grhl2. We note a significant effect of genetic background on the clft3 phenotype. One of these is a reduction in size of the telencephalon where we find abnormal patterns of neural progenitor mitosis and apoptosis in mutant brains. Interestingly, Grhl2 is not expressed in the developing forebrain, suggesting this is a survival factor for neural progenitors exerting a paracrine effect on the neural tissue from the overlying ectoderm where Grhl2 is highly expressed. genesis 53:573-582, 2015. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
authors.forename: Chelsea
authors.initials: C
authors.lastname: Menke
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
authors.forename: Megan
authors.initials: M
authors.lastname: Cionni
authors.affiliation: Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Department of Biology, Boston University, Massachusetts.
authors.forename: Trevor
authors.initials: T
authors.lastname: Siggers
authors.affiliation: Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Department of Pathology, Brigham & Women's Hospital and Harvard Medical School, Boston, Massachusetts.
authors.forename: Martha L
authors.initials: ML
authors.lastname: Bulyk
authors.affiliation: Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Seattle Children's Hospital, Center for Developmental Biology and Regenerative Medicine, Seattle, Washington.
authors.forename: David R
authors.initials: DR
authors.lastname: Beier
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.|Division of Genetics, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.|Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
authors.forename: Rolf W
authors.initials: RW
authors.lastname: Stottmann
keywords: ENU; cloning; development; forebrain; mouse; mutagenesis