title: Comparative Phytochemical Analysis of Essential Oils from Different Biological Parts of Artemisia herba alba and Their Cytotoxic Effect on Cancer Cells.
abstract: OBJECTIVE
  Carrying out the chemical composition and antiproliferative effects against cancer cells from different biological parts of Artemisia herba alba.
  
  
  METHODS
  Essential oils were studied by gas chromatography coupled to mass spectrometry (GC-MS) and their antitumoral activity was tested against P815 mastocytoma and BSR kidney carcinoma cell lines; also, in order to evaluate the effect on normal human cells, oils were tested against peripheral blood mononuclear cells PBMCs.
  
  
  RESULTS
  Essential oils from leaves and aerial parts (mixture of capitulum and leaves) were mainly composed by oxygenated sesquiterpenes 39.89% and 46.15% respectively; capitulum oil contained essentially monoterpenes (22.86%) and monocyclic monoterpenes (21.48%); esters constituted the major fraction (62.8%) of stem oil. Essential oils of different biological parts studied demonstrated a differential antiproliferative activity against P815 and BSR cancer cells; P815 cells are the most sensitive to the cytotoxic effect. Leaves and capitulum essential oils are more active than aerial parts. Interestingly, no cytotoxic effect of these essential oils was observed on peripheral blood mononuclear cells.
  
  
  CONCLUSIONS
  Our results showed that the chemical composition variability of essential oils depends on the nature of botanical parts of Artemisia herba alba. Furthermore, we have demonstrated that the differential cytotoxic effect depends not only on the essential oils concentration, but also on the target cells and the botanical parts of essential oils used.
authors.affiliation: Laboratory of Biological Engineering, Natural Substances, Cellular and Molecular Immuno-pharmacology, Immunobiology of Cancer Cells Cluster, Faculty of Science and Technology, Sultan Moulay Slimane University, Beni-Mellal, Morocco.
authors.forename: Mounir
authors.initials: M
authors.lastname: Tilaoui
authors.affiliation: Laboratory of Biological Engineering, Natural Substances, Cellular and Molecular Immuno-pharmacology, Immunobiology of Cancer Cells Cluster, Faculty of Science and Technology, Sultan Moulay Slimane University, Beni-Mellal, Morocco.
authors.forename: Hassan
authors.initials: H
authors.lastname: Ait Mouse
authors.affiliation: Laboratory of Biological Engineering, Natural Substances, Cellular and Molecular Immuno-pharmacology, Immunobiology of Cancer Cells Cluster, Faculty of Science and Technology, Sultan Moulay Slimane University, Beni-Mellal, Morocco.
authors.forename: Abdeslam
authors.initials: A
authors.lastname: Jaafari
authors.affiliation: Laboratory of Biological Engineering, Natural Substances, Cellular and Molecular Immuno-pharmacology, Immunobiology of Cancer Cells Cluster, Faculty of Science and Technology, Sultan Moulay Slimane University, Beni-Mellal, Morocco.
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Zyad
mesh_terms: D000328:Adult; D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D045744:Cell Line, Tumor; D006224:Cricetinae; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003603:Cytotoxins; D009822:Oils, Volatile; D013729:Terpenes