title: Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.).
abstract: A novel and highly effective source of anthracnose resistance in narrow-leafed lupin was identified. Resistance was shown to be governed by a single dominant locus. Molecular markers have been developed, which can be used for selecting resistant genotypes in lupin breeding. A screening for anthracnose resistance of a set of plant genetic resources of narrow-leafed lupin (Lupinus angustifolius L.) identified the breeding line Bo7212 as being highly resistant to anthracnose (Colletotrichum lupini). Segregation analysis indicated that the resistance of Bo7212 is inherited by a single dominant locus. The corresponding resistance gene was given the designation LanrBo. Previously published molecular anchor markers allowed us to locate LanrBo on linkage group NLL-11 of narrow-leafed lupin. Using information from RNAseq data obtained with inoculated resistant vs. susceptible lupin entries as well as EST-sequence information from the model genome Lotus japonicus, additional SNP and EST markers linked to LanrBo were derived. A bracket of two LanrBo-flanking markers allows for precise marker-assisted selection of the novel resistance gene in narrow-leafed lupin breeding programs.
authors.affiliation: Julius Kühn-Institut, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany. kristin.fischer@jki.bund.de.
authors.forename: Kristin
authors.initials: K
authors.lastname: Fischer
authors.affiliation: Saatzucht Steinach GmbH & Co KG, Bocksee, Germany.
authors.forename: Regine
authors.initials: R
authors.lastname: Dieterich
authors.affiliation: School of Plant Biology, The University of Western Australia, Crawley, Australia.|The UWA Institute of Agriculture, The University of Western Australia, Crawley, Australia.
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Nelson
authors.affiliation: The UWA Institute of Agriculture, The University of Western Australia, Crawley, Australia.|CSIRO, Agriculture Flagship, Wembley, Australia.
authors.forename: Lars G
authors.initials: LG
authors.lastname: Kamphuis
authors.affiliation: The UWA Institute of Agriculture, The University of Western Australia, Crawley, Australia.|CSIRO, Agriculture Flagship, Wembley, Australia.
authors.forename: Karam B
authors.initials: KB
authors.lastname: Singh
authors.affiliation: GenXPro GmbH, Frankfurt/Main, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Rotter
authors.affiliation: GenXPro GmbH, Frankfurt/Main, Germany.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Krezdorn
authors.affiliation: GenXPro GmbH, Frankfurt/Main, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Winter
authors.affiliation: Julius Kühn-Institut, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Wehling
authors.affiliation: Julius Kühn-Institut, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Ruge-Wehling
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002874:Chromosome Mapping; D020231:Colletotrichum; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D031297:Lupinus / Q000235:genetics* / Q000382:microbiology; D018895:Microsatellite Repeats; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers