title: Identification of hepatoprotective xanthones from the pericarps of Garcinia mangostana, guided with tert-butyl hydroperoxide induced oxidative injury in HL-7702 cells.
abstract: Bioactivity-guided fractionation of an ethanol-soluble extract from the pericarps of Garcinia mangostana, using tert-butyl hydroperoxide (t-BHP) induced oxidative damage in human normal hepatocytes (HL-7702), led to the identification of 10 known xanthones. Among them, γ-mangostin (γ-Man) exhibited the most potent activity to attenuate t-BHP induced hepatocyte injury. γ-Man significantly ameliorated t-BHP induced reactive oxygen species accumulation, mitochondrial membrane depolarization and cell nuclei morphology change in HL-7702 cells. t-BHP decreased the intracellular levels of key enzymes including glutamate oxaloacetate transaminase and glutamate pyruvate transaminase, which was totally reversed by γ-Man. Moreover, γ-Man significantly decreased the level of lipid peroxidation and increased the levels of superoxide dismutase and reduced glutathione, resulting in the alleviation of oxidative stress. The above results suggest γ-Man is a potential hepatoprotective agent against t-BHP induced oxidative injury, which may benefit the further application of G. mangostana as a health food.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China. ligenl@umac.mo.
authors.forename: Anqi
authors.initials: A
authors.lastname: Wang
authors.forename: Qianyu
authors.initials: Q
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Ye
authors.forename: Yitao
authors.initials: Y
authors.lastname: Wang
authors.forename: Ligen
authors.initials: L
authors.lastname: Lin
mesh_terms: D045744:Cell Line, Tumor; D005638:Fruit / Q000737:chemistry; D029762:Garcinia mangostana / Q000737:chemistry*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide / Q000633:toxicity
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D044004:Xanthones; D020122:tert-Butylhydroperoxide