title: Effect of microRNA-101 on apoptosis of rabbit condylar cartilage cells by inhibiting target gene SOX9.
abstract: OBJECTIVE
  To explore the effect of microRNA-101 on apoptosis of condylar cartilage cells and the specific mechanism of molecular biology.
  
  
  METHODS
  IL-1 was used to stimulate and establish the model of apoptosis of condylar cartilage cells. The expression change of miR-101 in control group was compared with that in IL-1 stimulation group by qRT-PCR. Overexpression and down-regulation models of miR-101 were established by transfecting Mimics and Inhibitor and verified by qRT-PCR. Flow cytometry was used to detect the effect of miR-101 overexpression and down-regulation on apoptosis. Target gene of miR-101 was analyzed and calculated through bioinformatics. Western blot and Luciferase report assay were used to detect whether SOX9 could become the target gene of miR-101.
  
  
  RESULTS
  qRT-PCR results showed that IL-1 stimulation could cause the increase of miR-101 expression. After the transfection of rabbit condylar cartilage cells by Mimics and Inhibitor, qRT-PCR results confirmed the significant effect of miR-101 overexpression and down-regulation. It was confirmed by flow cytometry that overexpression of miR-101 could promote the apoptosis of condylar cartilage cells, and down-regulation of miR-101 could reduce the apoptosis. It was confirmed by Western blot and Luciferase report assay that SOX9 was the target gene of miR-101, and miR-101 inhibited SOX9 expression through complementary pairing with 3'UTR of SOX9 mRNA.
  
  
  CONCLUSIONS
  miR-101 can promote the apoptosis of condylar cartilage cells through inhibiting the protein level of target gene SOX9.
authors.affiliation: Repair Department of Stomatology, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China. Electronic address: lilixin963@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Stomatology, Gansu Provincial Maternity and Child-care Hospital, Lanzhou 730050, Gansu Province, China.
authors.forename: Zi-Xin
authors.initials: ZX
authors.lastname: Wang
authors.affiliation: Repair Department of Stomatology, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Zi-Sheng
authors.initials: ZS
authors.lastname: Wang
authors.affiliation: Polymeric Chemistry and Physics, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Quan-Fang
authors.initials: QF
authors.lastname: Li
keywords: Apoptosis; Condyle cartilage; SOX9; miR-101