title: The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
abstract: Expression of mouse C-type lectin-like receptor 2 (CLEC-2) has been reported on circulating CD11b(high) Gr-1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC-2 expression failed to use CLEC-2-deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC-2-deficient adult mice using two independent approaches and employed two anti-mouse CLEC-2 antibody clones to investigate surface expression on hematopoietic cells from peripheral blood and secondary lymphoid organs. We rule out constitutive CLEC-2 expression on resting DCs and show that CLEC-2 is upregulated in response to LPS-induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC-2 and suggest that both circulating B lymphocytes and CD11b(high) Gr-1(high) myeloid cells lose CLEC-2 following entry into secondary lymphoid organs. These results have significant implications for our understanding of CLEC-2 physiological functions.
authors.affiliation: Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK.
authors.forename: Kate L
authors.initials: KL
authors.lastname: Lowe
authors.affiliation: Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK.
authors.forename: Leyre
authors.initials: L
authors.lastname: Navarro-Núñez
authors.affiliation: MRC Centre for Immune Regulation, University of Birmingham, Birmingham, UK.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bénézech
authors.affiliation: Centre for Translational Inflammation Research, Rheumatology Research Group, University of Birmingham, Birmingham, UK.
authors.forename: Saba
authors.initials: S
authors.lastname: Nayar
authors.affiliation: Centre for Translational Inflammation Research, Rheumatology Research Group, University of Birmingham, Birmingham, UK.|Medical School, University of Oxford, Oxford, UK.
authors.forename: Bethany L
authors.initials: BL
authors.lastname: Kingston
authors.affiliation: Department of Experimental Biomedicine, University Hospital, University of Würzburg, Würzburg, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Nieswandt
authors.affiliation: Centre for Translational Inflammation Research, Rheumatology Research Group, University of Birmingham, Birmingham, UK.
authors.forename: Francesca
authors.initials: F
authors.lastname: Barone
authors.affiliation: Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK.
authors.forename: Steve P
authors.initials: SP
authors.lastname: Watson
authors.affiliation: Centre for Translational Inflammation Research, Rheumatology Research Group, University of Birmingham, Birmingham, UK.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
authors.affiliation: Centre for Translational Inflammation Research, Rheumatology Research Group, University of Birmingham, Birmingham, UK.
authors.forename: Guillaume E
authors.initials: GE
authors.lastname: Desanti
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D001792:Blood Platelets / Q000276:immunology / Q000473:pathology; D039481:CD11b Antigen / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology*; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology; D037181:Lectins, C-Type / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics*; D008070:Lipopolysaccharides; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000276:immunology* / Q000473:pathology; D009928:Organ Specificity; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; C529518:CLEC-2 protein, mouse; C503133:Gr-1 protein, mouse; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D019707:Receptors, Chemokine
keywords: CLEC-2; Inflammation; Leucocytes; Mouse; Tamoxifen