title: New cembranoid diterpenes from the cultured octocoral Nephthea columnaris.
abstract: Two new 15-hydroxycembranoid diterpenes, 2β-hydroxy-7β,8α-epoxynephthenol (1) and 2β-hydroxy-11α,12β-epoxynephthenol (2), were isolated from extracts of the octocoral Nephthea columnaris along with a new natural cembrane, epoxynephthenol (3) and a known sterol, nephalsterol A (4). The structures of cembranes 1-3 were elucidated by spectroscopic methods and comparison of the spectroscopic data with those of related analogues. The cytotoxicity of metabolites 1-4 against a panel of tumor cells is also described.
authors.affiliation: Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 94450, Taiwan. hsiaoinon@gmail.com.|National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan. hsiaoinon@gmail.com.
authors.forename: Ting-Hsi
authors.initials: TH
authors.lastname: Hsiao
authors.affiliation: Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan. ma4200@cgmh.org.tw.
authors.forename: Ching-Hsiao
authors.initials: CH
authors.lastname: Cheng
authors.affiliation: Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. kuma0401@gmail.com.
authors.forename: Tung-Ying
authors.initials: TY
authors.lastname: Wu
authors.affiliation: Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 94450, Taiwan. jinx6609@nmmba.gov.tw.|National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan. jinx6609@nmmba.gov.tw.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan. wzh@mail.nsysu.edu.tw.|Department of Neurosurgery, Xiamen Chang Gung Memorial Hospital, Xiamen 361028, China. wzh@mail.nsysu.edu.tw.|Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. wzh@mail.nsysu.edu.tw.
authors.forename: Wu-Fu
authors.initials: WF
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. wzh@mail.nsysu.edu.tw.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: Institute of Oceanography, National Taiwan University, Taipei 10617, Taiwan. corallab@ntu.edu.tw.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.affiliation: Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. yachwu@mail.cmu.edu.tw.|School of Pharmacy, College of Pharmacy, China Medical University, Taichung 40402, Taiwan. yachwu@mail.cmu.edu.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. yachwu@mail.cmu.edu.tw.|Center for Molecular Medicine, China Medical University Hospital, Taichung 40447, Taiwan. yachwu@mail.cmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 94450, Taiwan. pjsung@nmmba.gov.tw.|National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan. pjsung@nmmba.gov.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. pjsung@nmmba.gov.tw.|Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. pjsung@nmmba.gov.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D020298:U937 Cells
chemical_list: D003603:Cytotoxins; D004224:Diterpenes
keywords: Nephthea columnaris; cembrane; cytotoxicity; nephalsterol; octocoral