title: A molecular Fe-complex as a catalyst probe for in-gel visual detection of proteins via signal amplification.
abstract: We report the use of a molecular peroxidase mimic biuret-Fe-TAML for chemoselective labeling of proteins and the subsequent visual detection (<0.1 pmoles) of the conjugate in a polyacrylamide gel by catalytic signal amplification. Use of this probe in activity based protein profiling (ABPP) of serine proteases is also demonstrated.
authors.affiliation: CReST Chemical Engineering Division, CSIR-National Chemical Laboratory, Pune-411008, India. ss.sengupta@ncl.res.in.
authors.forename: Sushma
authors.initials: S
authors.lastname: Kumari
authors.forename: Chakadola
authors.initials: C
authors.lastname: Panda
authors.forename: Shyamalava
authors.initials: S
authors.lastname: Mazumdar
authors.forename: Sayam
authors.initials: S
authors.lastname: Sen Gupta
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002417:Cattle; D005782:Gels / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D058085:Iron Compounds / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D005782:Gels; D058085:Iron Compounds; D011506:Proteins; D000099166:Polyacrylamides