title: Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures.
abstract: The combination of bone grafting materials with guided bone regeneration (GBR) membranes seems to provide promising results to restore bone defects in dental clinical practice. In the first part of this work, a novel protocol for decellularization and delipidation of bovine bone, based on multiple steps of thermal shock, washes with detergent and dehydration with alcohol, is described. This protocol is more effective in removal of cellular materials, and shows superior biocompatibility compared to other three methods tested in this study. Furthermore, histological and morphological analyses confirm the maintenance of an intact bone extracellular matrix (ECM). In vitro and in vivo experiments evidence osteoinductive and osteoconductive properties of the produced scaffold, respectively. In the second part of this study, two methods of bovine pericardium decellularization are compared. The osmotic shock-based protocol gives better results in terms of removal of cell components, biocompatibility, maintenance of native ECM structure, and host tissue reaction, in respect to the freeze/thaw method. Overall, the results of this study demonstrate the characterization of a novel protocol for the decellularization of bovine bone to be used as bone graft, and the acquisition of a method to produce a pericardium membrane suitable for GBR applications.
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gardin
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Ricci
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Ferroni
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Guazzo
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Sbricoli
authors.affiliation: Department of Animal Medicine, Productions and Health, University of Padova, Legnaro, Padova, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: De Benedictis
authors.affiliation: Department of Animal Medicine, Productions and Health, University of Padova, Legnaro, Padova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Finotti
authors.affiliation: Department of Animal Medicine, Productions and Health, University of Padova, Legnaro, Padova, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Isola
authors.affiliation: Department of Neurosciences, University of Padova, Padova, Italy.
authors.forename: Eriberto
authors.initials: E
authors.lastname: Bressan
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D005109:Extracellular Matrix; D005269:Femur; D010496:Pericardium; D051381:Rats; D012756:Sheep; D054457:Tissue Scaffolds