title: Identification and analysis of HSP70 from Sepiella maindroni under stress of Vibrio harveyi and Cd(2.).
abstract: The 70-kDa heat shock proteins (HSP70) play crucial roles in protecting cells against environmental stresses, such as heat shock, heavy metals and pathogenic bacteria. The full-length HSP70 cDNA of Sepiella maindroni (designated as SmHSP70, GenBank accession no. KJ739788) was 2109 bp, including an ORF of 1950 bp encoding a polypeptide of 649 amino acids with predicted pI/MW 5.24/71.30 kDa, a 62 bp-5'-UTR and a 97 bp-3'-UTR. BLASTp analysis and phylogenetic relationship strongly suggested that the amino acid sequence was a member of HSP70 family. Multiple sequence alignment revealed that SmHSP70 and other known HSP70 were highly conserved, especially in the regions of HSP70 family signatures, the bipartite nuclear targeting sequence, ATP/GTP-binding site motif and 'EEVD' motif. Time-dependent mRNA expression of SmHSP70 in the liver was recorded by quantitative real-time RT-PCR after Vibrio harveyi injection and Cd(2+) exposure. The results indicated that SmHSP70 played a significant role in mediating the environmental stress and immune response against pathogens.
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China. Electronic address: liuhuihui2004@126.com.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Liu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: He
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Chi
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China.
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Lv
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D049832:Decapodiformes / Q000187:drug effects / Q000235:genetics* / Q000821:virology*; D004777:Environment; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008099:Liver / Q000378:metabolism / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D014733:Vibrio / Q000472:pathogenicity*
chemical_list: D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D002104:Cadmium
keywords: Cd(2+) stress; Heat shock protein 70 (HSP70); Sepiella maindroni; Vibrio infection