title: A rescue act: Translesion DNA synthesis past N(2) -deoxyguanosine adducts.
abstract: Genomic DNA is continually subjected to a number of chemical insults that result in the formation of modified nucleotides--termed as DNA lesions. The N(2) -atom of deoxyguanosine is particularly reactive and a number of chemicals react at this site to form different kinds of DNA adducts. The N(2) -deoxyguanosine adducts perturb different genomic processes and are particularly deleterious for DNA replication as they have a strong tendency to inhibit replicative DNA polymerases. Many organisms possess specialized dPols--generally classified in the Y-family--that serves to rescue replication stalled at N(2) -dG and other adducts. A review of minor groove N(2) -adducts and the known strategies utilized by Y-family dPols to replicate past these lesions will be presented here.
authors.affiliation: Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, Haryana, 121 001, India.
authors.forename: Deepak T
authors.initials: DT
authors.lastname: Nair
authors.affiliation: Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, Haryana, 121 001, India.|Manipal University, Manipal.Edu, Manipal, 576104, Karnataka, India.
authors.forename: Jithesh
authors.initials: J
authors.lastname: Kottur
authors.affiliation: Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, Haryana, 121 001, India.|Manipal University, Manipal.Edu, Manipal, 576104, Karnataka, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Sharma
mesh_terms: D002384:Catalysis; D004247:DNA / Q000096:biosynthesis*; D018736:DNA Adducts / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D019951:DNA Polymerase beta / Q000737:chemistry / Q000378:metabolism; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D000094769:DNA Polymerase iota; D000099270:Y-Family DNA Polymerases
chemical_list: D004247:DNA; D018736:DNA Adducts; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase; D003849:Deoxyguanosine; D029968:Escherichia coli Proteins; D009687:Nuclear Proteins; D009713:Nucleotidyltransferases; D000094769:DNA Polymerase iota; C117629:8-nitro-2'-deoxyguanosine; C509838:Dpo4 protein, E coli; C429004:REV1 protein, human; D000099270:Y-Family DNA Polymerases
keywords: DNA polymerase; DNA replication; N2-adducts; translesion DNA synthesis