title: An actin-dependent spindle position checkpoint ensures the asymmetric division in mouse oocytes.
abstract: Faithful chromosome segregation, during meiosis, is of critical importance to prevent aneuploidy in the resulting embryo. In mammalian oocytes, the segregation of homologous chromosomes takes place with the spindle located at the cell's periphery. The spindle is often assembled close to the centre of the cell, which necessitates the actin network for spindle transport to the cell cortex. In this study, we investigate how the segregation of chromosomes is coordinated with the positioning of the metaphase I spindle. We develop different assays to perturb the spindle's position and to delay its relocation to the cell periphery. We find that anaphase is delayed until the spindle is positioned in close proximity with the oocyte cortex. We further show that the metaphase arrest is dependent on a functional actin network, in addition to the spindle assembly checkpoint. Our work provides the first evidence for the existence of a functional spindle position checkpoint.
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Aïcha
authors.initials: A
authors.lastname: Metchat
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Eguren
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Julius M
authors.initials: JM
authors.lastname: Hossain
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Antonio Z
authors.initials: AZ
authors.lastname: Politi
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Huet
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D060049:Asymmetric Cell Division; D059447:Cell Cycle Checkpoints; D020090:Chromosome Segregation; D049452:Green Fluorescent Proteins; D059566:M Phase Cell Cycle Checkpoints; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism; D009865:Oocytes / Q000166:cytology*; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: D000199:Actins; D049452:Green Fluorescent Proteins