title: Short Communication: Increase of Sialylated N-Glycansin Eyes with Neovascular Glaucoma Secondary to Proliferative Diabetic Retinopathy.
abstract: To investigate the alteration of N-glycans in the vitreous fluid of patients with neovascular glaucoma (NVG) secondary to proliferative diabetic retinopathy (PDR).
  
  
  
  Vitreous samples were collected from 18 patients with PDR (including 7 with NVG and 11 without NVG), and 17 patients without diabetes. Profiles of N-glycans were analyzed by glycoblotting-based high throughput protocol, which we recently developed. Protein levels of vascular endothelial growth factor (VEGF)-A were measured by ELISA.
  
  
  
  The concentration of total N-glycans and the concentration of N-glycans with sialic acids were significantly higher in NVG group compared with those in non-NVG group or control group, whereas there was no significant difference in concentrations of high-mannose N-glycans among three groups. There was a moderate correlation between the concentrations of sialylated N-glycans and VEGF-A.
  
  
  
  Our data demonstrate the distinct changes of N-glycan profile and the increase of sialylated N-glycans in eyes with NVG secondary to PDR.
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan .|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan .|c Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University , Sapporo , Japan and.
authors.forename: Saori
authors.initials: S
authors.lastname: Inafuku
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan .|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan .
authors.forename: Kousuke
authors.initials: K
authors.lastname: Noda
authors.affiliation: c Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University , Sapporo , Japan and.|d Medicinal Chemistry Pharmaceuticals, Co. Ltd. , Sapporo , Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Amano
authors.affiliation: c Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University , Sapporo , Japan and.|d Medicinal Chemistry Pharmaceuticals, Co. Ltd. , Sapporo , Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan .|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan .
authors.forename: Susumu
authors.initials: S
authors.lastname: Ishida
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000150:complications* / Q000175:diagnosis / Q000378:metabolism; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015355:Glaucoma, Neovascular / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D011134:Polysaccharides; D042461:Vascular Endothelial Growth Factor A
keywords: N-glycans; neovascular glaucoma; proliferative diabetic retinopathy