title: Different Types of Peptide Detected by Mass Spectrometry among Fresh Silk and Archaeological Silk Remains for Distinguishing Modern Contamination.
abstract: Archaeological silk provides abundant information for studying ancient technologies and cultures. However, due to the spontaneous degradation and the damages from burial conditions, most ancient silk fibers which suffered the damages for thousands of years were turned into invisible molecular residues. For the obtained rare samples, extra care needs to be taken to accurately identify the genuine archaeological silk remains from modern contaminations. Although mass spectrometry (MS) is a powerful tool for identifying and analyzing the ancient protein residues, the traditional approach could not directly determine the dating and contamination of each sample. In this paper, a series of samples with a broad range of ages were tested by MS to find an effective and innovative approach to determine whether modern contamination exists, in order to verify the authenticity and reliability of the ancient samples. The new findings highlighted that the detected peptide types of the fibroin light chain can indicate the degradation levels of silk samples and help to distinguish contamination from ancient silk remains.
authors.affiliation: Basic Research Center of Heritage Conservation Science, Department for History of Science and Scientific Archaeology, University of Science and Technology of China, Hefei, China 230026.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Basic Research Center of Heritage Conservation Science, Department for History of Science and Scientific Archaeology, University of Science and Technology of China, Hefei, China 230026.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, China 230026.
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
authors.affiliation: Basic Research Center of Heritage Conservation Science, Department for History of Science and Scientific Archaeology, University of Science and Technology of China, Hefei, China 230026.
authors.forename: Decai
authors.initials: D
authors.lastname: Gong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001106:Archaeology; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*
chemical_list: D010455:Peptides; D047011:Silk