title: Effect of rhBMP-2 Immobilized Anorganic Bovine Bone Matrix on Bone Regeneration.
abstract: Anorganic bovine bone matrix (Bio-Oss®) has been used for a long time for bone graft regeneration, but has poor osteoinductive capability. The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) has been suggested to overcome this limitation of Bio-Oss®. In the present study, heparin-mediated rhBMP-2 was combined with Bio-Oss® in animal experiments to investigate bone formation performance; heparin was used to control rhBMP-2 release. Two calvarial defects (8 mm diameter) were formed in a white rabbit model and then implanted or not (controls) with Bio-Oss® or BMP-2/Bio-Oss®. The Bio-Oss® and BMP-2/Bio-Oss® groups had significantly greater new bone areas (expressed as percentages of augmented areas) than the non-implanted controls at four and eight weeks after surgery, and the BMP-2/Bio-Oss® group (16.50 ± 2.87 (n = 6)) had significantly greater new bone areas than the Bio-Oss® group (9.43 ± 3.73 (n = 6)) at four weeks. These findings suggest that rhBMP-2 treated heparinized Bio-Oss® markedly enhances bone regeneration.
authors.affiliation: Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, YangSan 676-870, Korea. huhjb@pusan.ac.kr.
authors.forename: Jung-Bo
authors.initials: JB
authors.lastname: Huh
authors.affiliation: Advanced Prosthodontics, Postgraduate School of Clinical Dentistry, Institute for Clinical Dental Research, Korea University, Seoul 152-703, Korea. gumcab@hanmail.net.
authors.forename: June-Jip
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: Research Development Institute, Cowellmedi Co., Ltd., Busan 617-801, Korea. ckh@cowellmedi.com.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Choi
authors.affiliation: Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, YangSan 676-870, Korea. bjhyeon@gmail.com.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Bae
authors.affiliation: Advanced Prosthodontics, Postgraduate School of Clinical Dentistry, Institute for Clinical Dental Research, Korea University, Seoul 152-703, Korea. wddc@korea.ac.kr.
authors.forename: Jeong-Yeol
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital, Seoul 152-703, Korea.
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Advanced Prosthodontics, Postgraduate School of Clinical Dentistry, Institute for Clinical Dental Research, Korea University, Seoul 152-703, Korea. swshin@korea.ac.kr.
authors.forename: Sang-Wan
authors.initials: SW
authors.lastname: Shin
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D004195:Disease Models, Animal; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D018613:Microscopy, Confocal; D008903:Minerals / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D012886:Skull / Q000473:pathology; D013499:Surface Properties; D014057:Tomography, X-Ray Computed; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: C077540:Bio-Oss; D055396:Bone Morphogenetic Protein 2; D055767:Immobilized Proteins; D008903:Minerals; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2
keywords: Bio-Oss®; anorganic bovine bone; bone regeneration; rhBMP-2