title: Importin-α7 Is Involved in the Formation of Ebola Virus Inclusion Bodies but Is Not Essential for Pathogenicity in Mice.
abstract: Ebola virus (EBOV) protein 24 antagonizes the host interferon (IFN) response by hijacking select nuclear importin-α isoforms. Thereby, it blocks STAT1-mediated IFN-α/β and IFN-γ synthesis. However, owing to the lack of importin-α knockout animal models in the past, their role in EBOV pathogenesis remained largely unknown. Here, we demonstrate that importin-α7 is involved in the formation of EBOV inclusion bodies and replication. However, deletion of the gene encoding importin-α7 was not sufficient to increase survival rates among mice infected with EBOV.
authors.affiliation: Viral Zoonoses and Adaptation, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg Center for Structural and Cellular Biology in Medicine, Institute of Biology, University of Lübeck, Germany.
authors.forename: Gülsah
authors.initials: G
authors.lastname: Gabriel
authors.affiliation: Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
authors.forename: Friederike
authors.initials: F
authors.lastname: Feldmann
authors.affiliation: Viral Zoonoses and Adaptation, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg.
authors.forename: Rudolph
authors.initials: R
authors.lastname: Reimer
authors.affiliation: Viral Zoonoses and Adaptation, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg.
authors.forename: Swantje
authors.initials: S
authors.lastname: Thiele
authors.affiliation: Viral Zoonoses and Adaptation, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg.
authors.forename: Meike
authors.initials: M
authors.lastname: Fischer
authors.affiliation: Center for Structural and Cellular Biology in Medicine, Institute of Biology, University of Lübeck, Germany.
authors.forename: Enno
authors.initials: E
authors.lastname: Hartmann
authors.affiliation: Center for Structural and Cellular Biology in Medicine, Institute of Biology, University of Lübeck, Germany Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bader
authors.affiliation: Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ebihara
authors.affiliation: Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hoenen
authors.affiliation: Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004261:DNA Replication / Q000235:genetics; D029043:Ebolavirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D019142:Hemorrhagic Fever, Ebola / Q000378:metabolism* / Q000821:virology*; D007181:Inclusion Bodies, Viral / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism; D014774:Virulence / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics; D028901:alpha Karyopherins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D028901:alpha Karyopherins
keywords: Ebola virus; STAT1; VP24; importin-α; inclusion bodies; interferon; pathogenicity