title: [Effects of L-carnitine and bisoprolol on endoplasmic reticulum stress-mediated myocardial injury after cardiopulmonary resuscitation in rats].
abstract: OBJECTIVE
  To explore the effects of L-carnitine and bisoprolol on endoplasmic reticulum stress-mediated myocardial injury after cardiopulmonary resuscitation in rats.
  
  
  METHODS
  A total of 75 Sprague-Dawley rats were randomly divided into 5 groups of sham operation (sham); cardiopulmonary resuscitation (CR), L-carnitine (L), bisoprolol treatment (B) and L-carnitine and bisoprolol treatment (LB). Myocardial pathological changes were detected by hematoxylin and eosin, myocardial apoptosis by terminal deoxynucleotidyl transferase mediated dUTP nick and the expressions of key factors in endoplasmic reticulum (ER) by Western blot.
  
  
  RESULTS
  Compared with sham group, apoptosis and pathological lesions significantly increased in other groups (P < 0.05). And the levels of GRP78, CHOP and Caspase-12 were significantly higher (P < 0.05). The expression of ER factor protein and the degrees of myocardial injury in LB and B groups decreased compared with CR group. And LB group was the most obvious.
  
  
  CONCLUSIONS
  Combined use of levocarnitine and bisoprolol exerts protective effects on cardiopulmonary resuscitation in rats. And the mechanism may be related to an inhibition of ER stress.
authors.affiliation: EICU, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Meiqi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: EICU, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Wu
authors.affiliation: EICU, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Shen
authors.affiliation: EICU, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.affiliation: EICU, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Tu
authors.affiliation: EICU, Zhejiang Provincial People's Hospital, Hangzhou 310014, China; Email: yesterdaygun@126.com.
authors.forename: Changlin
authors.initials: C
authors.lastname: Zhai
mesh_terms: D000818:Animals; D017209:Apoptosis; D017298:Bisoprolol; D016887:Cardiopulmonary Resuscitation; D002331:Carnitine; D053454:Caspase 12; D004721:Endoplasmic Reticulum; D059865:Endoplasmic Reticulum Stress; D006335:Heart Injuries; D006360:Heat-Shock Proteins; D008168:Lung; D009206:Myocardium; D051381:Rats; D017207:Rats, Sprague-Dawley; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D017298:Bisoprolol; D002331:Carnitine; D053454:Caspase 12; D006360:Heat-Shock Proteins; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP; C506443:Casp12 protein, rat