title: Identification of novel targets for seasonal allergic rhinitis during and outside the pollen season by microarray analysis.
abstract: CONCLUSIONS
  FOS, JUN, and DUSP1 could be used as candidate targets for the treatment of seasonal allergic rhinitis (SAR) during the pollen season, while KLF4 and CD163 could be used as candidate targets outside the pollen season.
  
  
  OBJECTIVE
  The aim of this study is to screen novel genes related to SAR during and outside the pollen season, by using microarray analysis.
  
  
  METHODS
  The mRNA expression profile (GSE50101) of CD4(+) T cells from SAR and healthy controls during and outside the pollen season was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) were then screened with the cut-off criteria of fold change larger than 1.5 and p-value less than 0.05.
  
  
  RESULTS
  A total of 47 DEGs were identified. Ten DEGs were shared by SAR patient samples during and outside the pollen season, while four and 23 DEGs were specific to during and outside the pollen season, respectively. Five miRNAs were screened in this study. Among these miRNAs, miR-139, miR-101, miR-29A, and miR-181 could target FOS; miR-200 and miR-29A could target KLF4; miR-101 and miR-200 could target DUSP1; miR-139 and miR-181 could target JUN and CD163, respectively.
authors.affiliation: a 1 Department of Otolaryngology, Nanjing University of Chinese Medicine, Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated with Nanjing University of Chinese Medicine , Nanjing City, Jiangsu Province, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: b 2 Department of Otolaryngology, Affiliated Hospital of Nanjing University of Chinese Medicine , Nanjing City, Jiangsu Province, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.affiliation: b 2 Department of Otolaryngology, Affiliated Hospital of Nanjing University of Chinese Medicine , Nanjing City, Jiangsu Province, PR China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Chen
mesh_terms: D000485:Allergens; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D000090062:Kruppel-Like Factor 4; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods*; D011058:Pollen; D012333:RNA, Messenger / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism
chemical_list: D000485:Allergens; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Seasonal allergic rhinitis; differentially expressed genes; microRNA; network analysis