title: Effect of bortezomib on migration and invasion in cervical carcinoma HeLa cell.
abstract: OBJECTIVE
  To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism.
  
  
  METHODS
  The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot.
  
  
  RESULTS
  MTT assay indicated bortezomib (2.5 μM, 5 μM, 10 μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48 h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9.
  
  
  CONCLUSIONS
  These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
authors.affiliation: Department of Genetic Reproduction, Maternal and Child Health Hospital, Tangshan 063000, China. Electronic address: shichong2014@126.com.
authors.forename: Chong
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Graduate, Hebei United University, Tangshan 063000, China.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Zhang
authors.affiliation: Pharmacy Department, Affiliated Hospital of North China Coal Medical University, Tangshan 063000, China.
authors.forename: Shu-Wang
authors.initials: SW
authors.lastname: Yin
keywords: Akt/mTOR signal pathway; Bortezomib; Cervical carcinoma HeLa cell; Invasion; Migration