title: TIGAR overexpression diminishes radiosensitivity of parotid gland fibroblast cells and inhibits IR-induced cell autophagy.
abstract: Our previous study proved that TP53-induced glycolysis and apoptosis regulator (TIGAR) abrogation is able to radiosensitize glioma cells. Whether TIGAR over-expression has radio-protective effect in human parotid gland cells is still unknown. In this study human parotid gland fibroblast Hs 917.T cells were transfected with pcDNA3.1-TIGAR, and clonogenic assay was performed to investigate the radiosensitivity of Hs 917.T cells over-expressing pcDNA3.1 or pcDNA3.1-TIGAR. Western blot was carried out to demonstrate the autophagy activity of cells being irradiated, and immunofluorescence assay was used to evaluate the DNA damage repair process of irradiated Hs 917.T cells. It was revealed that TIGAR over-expression could diminish the radiosensitivity of Hs 917.T cells, and the autophagy level induced by ionizing radiation (IR) was also decreased by TIGAR transfection. The mechanism might rely on TIGAR over-expression induced ROS scavenging and NADPH increasing. Using autophagy inhibitor, it was also elaborated that IR-induced autophagy in Hs 917.T cells was protective autophagy but not traumatic autophagy.
authors.affiliation: Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdiscip Linary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Suzhou 215123, China ; Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University Nantong 226321, China.
authors.forename: Guomei
authors.initials: G
authors.lastname: Tai
authors.affiliation: Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdiscip Linary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Suzhou 215123, China.
authors.forename: Haowen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdiscip Linary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Suzhou 215123, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University Nantong 226321, China.
authors.forename: Guojian
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdiscip Linary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Suzhou 215123, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdiscip Linary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Suzhou 215123, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University Nantong 226321, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdiscip Linary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Suzhou 215123, China.
authors.forename: Fenju
authors.initials: F
authors.lastname: Liu
mesh_terms: D051017:Apoptosis Regulatory Proteins; D001343:Autophagy / Q000235:genetics*; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D010306:Parotid Gland / Q000166:cytology* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases; D011836:Radiation Tolerance / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D017382:Reactive Oxygen Species; D010744:Phosphoric Monoester Hydrolases; C512708:TIGAR protein, human
keywords: TIGAR; autophagy; parotid gland cells