title: Electrophoretic Mobility Shift Assays.
abstract: Experimental demonstration of regulatory protein interactions with the sequences upstream of potential target genes is an important element in gene expression studies. These experiments termed electrophoretic mobility shift assays (EMSAs) provide valuable insight into the mechanism of action of transcription factors. EMSAs combined with downstream applications such as transcriptional analysis help uncover precisely how regulatory proteins control target gene expression. This chapter comprises a guideline for expression and purification of recombinant transcription factor proteins followed by a detailed protocol for EMSAs.
authors.affiliation: Antimicrobial Discovery Center, Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA, 02115, USA. s.rowe@neu.edu.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Rowe
authors.affiliation: Department of Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland. jamesp.ogara@nuigalway.ie.
authors.forename: James P
authors.initials: JP
authors.lastname: O'Gara
mesh_terms: D001665:Binding Sites / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000235:genetics; D060066:Protein Interaction Maps / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D014157:Transcription Factors
keywords: Electrophoretic mobility shift assay; Gene regulation; Promoter; Transcription regulator