title: The Role of NF mRNA and Calpain in NF Reduction of Acrylamide Neuropathy.
abstract: The purpose of this study was to study the role of neurofilament (NF) mRNA and calpain in NF reduction of acrylamide (ACR) neuropathy. Male Wistar adult rats were injected i.p. every other day with ACR (20 mg/kg·bW or 40 mg/kg·bW) for 8 weeks. NF mRNA expression was detected using RT-PCR and the calpain concentration was determined using western blot analysis. The NF mRNA expression significantly decreased while the level of m-calpain and μ-calpain significantly increased in two ACR-treated rats groups regardless of the ACR dose. The light NF (NF-L) protein expression was significantly correlated with NF-L mRNA expression. Combined with previous data, the concentrations of three NF subunits were negatively correlated with the calpain levels. These findings suggest that NF-L mRNA and calpain mediated the reduction in NF of ACR neuropathy.
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, Shandong, China.
authors.forename: Su Fang
authors.initials: SF
authors.lastname: Yu
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, Shandong, China.
authors.forename: Xiao Min
authors.initials: XM
authors.lastname: Wei
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, Shandong, China.
authors.forename: Feng Feng
authors.initials: FF
authors.lastname: Yan
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, Shandong, China.
authors.forename: Shu E
authors.initials: SE
authors.lastname: Wang
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, Shandong, China.
authors.forename: Cui Li
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, Shandong, China.
authors.forename: Xi Wei
authors.initials: XW
authors.lastname: Yang
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D002154:Calpain / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D007382:Intermediate Filaments / Q000235:genetics*; D008297:Male; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D012333:RNA, Messenger; D020106:Acrylamide; D002154:Calpain