title: Rapidly light-activated surgical protein glue inspired by mussel adhesion and insect structural crosslinking.
abstract: Currently approved surgical tissue glues do not satisfy the requirements for ideal bioadhesives due to limited adhesion in wet conditions and severe cytotoxicity. Herein, we report a new light-activated, mussel protein-based bioadhesive (LAMBA) inspired by mussel adhesion and insect dityrosine crosslinking chemistry. LAMBA exhibited substantially stronger bulk wet tissue adhesion than commercially available fibrin glue and good biocompatibility in both in vitro and in vivo studies. Besides, the easily tunable, light-activated crosslinking enabled an effective on-demand wound closure and facilitated wound healing. Based on these outstanding properties, LAMBA holds great potential as an ideal surgical tissue glue for diverse medical applications, including sutureless wound closures of skin and internal organs.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jeon
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea; Division of Bioengineering, Incheon National University, Incheon 406-772, South Korea.
authors.forename: Byeong Hee
authors.initials: BH
authors.lastname: Hwang
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Yang
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Bum Jin
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Bong-Hyuk
authors.initials: BH
authors.lastname: Choi
authors.affiliation: Department of Plastic Surgery, College of Medicine, Dongguk University, Gyeongju 780-714, South Korea.
authors.forename: Gyu Yong
authors.initials: GY
authors.lastname: Jung
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea. Electronic address: hjcha@postech.ac.kr.
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003487:Cyanoacrylates / Q000494:pharmacology; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D007313:Insecta / Q000737:chemistry*; D008027:Light; D011506:Proteins / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D034421:Sus scrofa; D013537:Sutures; D014014:Tissue Adhesives / Q000494:pharmacology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D011506:Proteins; D011994:Recombinant Proteins; D014014:Tissue Adhesives; C037403:adhesive protein, mussel; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D014443:Tyrosine; C007543:dityrosine
keywords: Dityrosine crosslinks; Light-activated hydrogel; Mussel adhesive protein; Protein adhesive; Tissue adhesive