title: Effects of Different Zinc Species on Cellar Zinc Distribution, Cell Cycle, Apoptosis and Viability in MDAMB231 Cells.
abstract: Intracellular metal elements exist in mammalian cells with the concentration range from picomoles per litre to micromoles per litre and play a considerable role in various biological procedures. Element provided by different species can influence the availability and distribution of the element in a cell and could lead to different biological effects on the cell's growth and function. Zinc as an abundant and widely distributed essential trace element, is involved in numerous and relevant physiological functions. Zinc homeostasis in cells, which is regulated by metallothioneins, zinc transporter/SLC30A, Zrt-/Irt-like proteins/SLC39A and metal-response element-binding transcription factor-1 (MTF-1), is crucial for normal cellular functioning. In this study, we investigated the influences of different zinc species, zinc sulphate, zinc gluconate and bacitracin zinc, which represented inorganic, organic and biological zinc species, respectively, on cell cycle, viability and apoptosis in MDAMB231 cells. It was found that the responses of cell cycle, apoptosis and death to different zinc species in MDAMB231 cells are different. Western blot analysis of the expression of several key proteins in regulating zinc-related transcription, cell cycle, apoptosis, including MTF-1, cyclin B1, cyclin D1, caspase-8 and caspase-9 in treated cells further confirmed the observed results on cell level.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing, 210093, China.
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing, 210093, China.
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, 22 Hankou Road, Nanjing, 210093, China. wjzheng@nju.edu.cn.
authors.forename: Wei-juan
authors.initials: WJ
authors.lastname: Zheng
authors.affiliation: MOE Key Laboratory of Modern Toxicology, School of Public Health, Nanjing Medical University, 818 East Tian-Yuan Road, Nanjing, 211166, China.
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing, 210093, China. hzlian@nju.edu.cn.
authors.forename: Hong-zhen
authors.initials: HZ
authors.lastname: Lian
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing, 210093, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, 22 Hankou Road, Nanjing, 210093, China.
authors.forename: Zi-chun
authors.initials: ZC
authors.lastname: Hua
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002451:Cell Compartmentation; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D018613:Microscopy, Confocal; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D015032:Zinc
keywords: Apoptosis; Cell cycle; Cell viability; Zinc species