title: Characterization of a novel adenovirus isolated from a skunk.
abstract: Adenoviruses are a ubiquitous group of viruses that have been found in a wide range of hosts. A novel adenovirus from a skunk suffering from acute hepatitis was isolated and its DNA genome sequenced. The analysis revealed this virus to be a new member of the genus Mastadenovirus, with a genome of 31,848 bp in length containing 30 genes predicted to encode proteins, and with a G+C content of 49.0%. Global genomic organization indicated SkAdV-1 was similar in organization to bat and canine adenoviruses, and phylogenetic comparison suggested these viruses shared a common ancestor. SkAdV-1 demonstrated an ability to replicate in several mammalian liver cell lines suggesting a potential tropism for this virus.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Kozak
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: James G
authors.initials: JG
authors.lastname: Ackford
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Slaine
authors.affiliation: Public Health Ontario, Toronto, Ontario, Canada.
authors.forename: Aimin
authors.initials: A
authors.lastname: Li
authors.affiliation: Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Susy
authors.initials: S
authors.lastname: Carman
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Doug
authors.initials: D
authors.lastname: Campbell
authors.affiliation: Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada.
authors.forename: M Katherine
authors.initials: MK
authors.lastname: Welch
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada. Electronic address: enagy@uoguelph.ca.
authors.forename: Éva
authors.initials: É
authors.lastname: Nagy
mesh_terms: D000208:Acute Disease; D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D045744:Cell Line, Tumor; D002685:Chiroptera; D004285:Dogs; D005260:Female; D059646:Genome Size; D016679:Genome, Viral; D006524:Hepatitis, Viral, Animal / Q000473:pathology / Q000821:virology*; D008099:Liver / Q000473:pathology / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D017921:Mastadenovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012889:Mephitidae / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D056189:Viral Tropism; D014779:Virus Replication
keywords: Liver tropism; Mastadenovirus; Skunk adenovirus; Whole genome sequence