title: Pretreatment of in vitro matured bovine oocytes with docetaxel before vitrification: Effects on cytoskeleton integrity and developmental ability after warming.
abstract: The stabilization of spindle fibersis important for successful vitrification of bovine oocytes because microtubules and other cytoskeleton fibers (CSF) can be damaged during vitrification, resulting in failure of fertilization after thawing. Docetaxel, a stabilizing agent, could potentially reduce CSF damage of bovine oocytes induced during vitrification. However, there have been no reports on the effects of docetaxel on their vitrification. Experiment 1 was conducted to investigate the effects of various doses of docetaxel (0.0, 0.05, 0.5, 5.0 and 50 μM) in preincubation medium of in vitro matured (IVM) bovine oocytes on their developmental ability after in vitro fertilization (IVF). The results show that 0.05 μM docetaxel had no adverse effect on embryo development, while docetaxel at a concentration of ⩾0.5 μM inhibited development. Experiments 2 and 3 were conducted to investigate the effects of preincubation of IVM bovine oocytes with 0.05 μM docetaxel for 30 min prior to vitrification-warming on CSF integrity (Experiment 2), and on oocyte survival and viability after IVF (Experiment 3). When preincubated with 0.05 μM docetaxel for 30 min before vitrification, post-thawed oocytes had less CSF damage and higher survival rates compared with those untreated with docetaxel before vitrification. Surviving oocytes also had higher rates of cleavage and development to the blastocyst stage after IVF. In conclusion, preincubation of IVM bovine oocytes with 0.05 μM docetaxel for 30 min before vitrification was effective at preventing CSF damage during vitrification, and improving oocyte viability after warming and subsequent cleavage and blastocyst formation after IVF.
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Jakkhaphan
authors.initials: J
authors.lastname: Chasombat
authors.affiliation: Food and Fertilizer Technology Center, Taipei 10648, Taiwan; NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Rangsun
authors.initials: R
authors.lastname: Parnpai
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: vthevi@kku.ac.th.
authors.forename: Thevin
authors.initials: T
authors.lastname: Vongpralub
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003599:Cytoskeleton / Q000187:drug effects; D000077143:Docetaxel; D047108:Embryonic Development / Q000187:drug effects; D005307:Fertilization in Vitro / Q000187:drug effects* / Q000379:methods; D059471:In Vitro Oocyte Maturation Techniques; D008870:Microtubules / Q000502:physiology; D009865:Oocytes / Q000187:drug effects*; D043823:Taxoids / Q000494:pharmacology*; D058989:Vitrification / Q000187:drug effects*
chemical_list: D043823:Taxoids; D000077143:Docetaxel
keywords: Bovine oocytes; Cytoskeleton fibers; Docetaxel; Vitrification