title: Ultrasound assisted lipase catalyzed synthesis of cinnamyl acetate via transesterification reaction in a solvent free medium.
abstract: Cinnamyl acetate is known for its use as flavor and fragrance material in different industries such as food, pharmaceutical, cosmetic etc. This work focuses on ultrasound assisted lipase (Novozym 435) catalyzed synthesis of cinnamyl acetate via transesterification of cinnamyl alcohol and vinyl acetate in non-aqueous, solvent free system. Optimization of various parameters shows that a higher yield of 99.99% can be obtained at cinnamyl alcohol to vinyl acetate ratio of 1:2 with 0.2% of catalyst, at 40°C and 150 rpm, with lower ultrasound power input of 50 W (Ultrasound intensity 0.81 W/cm(2)), at 25 kHz frequency, 50% duty cycle. Further, the time required for the maximum conversion is reduced to 20 min as compared to 60 min of conventional process. Similarly, the enzyme can be successfully reused seven times without loss of enzyme activity. Thus, ultrasound helps to enhance the enzyme catalyzed synthesis of flavors.
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
authors.forename: Prerana D
authors.initials: PD
authors.lastname: Tomke
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India. Electronic address: vk.rathod@ictmumbai.edu.in.
authors.forename: Virendra K
authors.initials: VK
authors.lastname: Rathod
mesh_terms: D055162:Biocatalysis; D002934:Cinnamates / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D000069453:Ultrasonic Waves
chemical_list: D002934:Cinnamates; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; C093667:Novozyme 435; D008049:Lipase; C526329:cinnamyl acetate
keywords: Cinnamyl acetate; Cinnamyl alcohol; Novozym 435; Transesterification; Ultrasound; Vinyl acetate