title: Epitope mapping of 7S cashew antigen in complex with antibody by solution-phase H/D exchange monitored by FT-ICR mass spectrometry.
abstract: The potential epitope of a recombinant food allergen protein, cashew Ana o 1, reactive to monoclonal antibody, mAb 2G4, has been mapped by solution-phase amide backbone H/D exchange (HDX) monitored by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Purified mAb 2G4 was incubated with recombinant Ana o 1 (rAna o 1) to form antigen:monoclonal antibody (Ag:mAb) complexes. Complexed and uncomplexed (free) rAna o 1 were then subjected to HDX-MS analysis. Five regions protected from H/D exchange upon mAb binding are identified as potential conformational epitope-contributing segments.
authors.affiliation: National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, FL, 32310, USA.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guan
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL, 32306, USA.
authors.forename: Kyle A
authors.initials: KA
authors.lastname: Noble
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, FL, 32306, USA.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL, 32306, USA.
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Roux
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, FL, 32306, USA.
authors.forename: Shridhar K
authors.initials: SK
authors.lastname: Sathe
authors.affiliation: National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, FL, 32310, USA.
authors.forename: Nicolas L
authors.initials: NL
authors.lastname: Young
authors.affiliation: National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, FL, 32310, USA.|Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, FL, 32306, USA.
authors.forename: Alan G
authors.initials: AG
authors.lastname: Marshall
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D031021:Anacardium / Q000737:chemistry*; D041961:Deuterium Exchange Measurement / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000485:Allergens; D000939:Epitopes; D055314:Seed Storage Proteins
keywords: FTMS; allergen; hydrogen/deuterium exchange; protein complex