title: Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens.
abstract: BACKGROUND
  Hsf4 is closely related to the development of cataract. However, the molecular mechanisms remain unknown. This study aimed to explore the molecular mechanisms that how Hsf4 mutations influence development of lens and thus lead to cataract in mouse.
  
  
  METHODS
  The mRNA expression profile of mouse tissue samples from Hsf4-null and wile-type lenses was downloaded from Gene Expression Omnibus database. Then the LIMMA package was used to screen differentially expressed genes (DEGs) and DAVID was applied to identify the significantly enriched Gene Ontology (GO) categories for DEGs. Furthermore, the protein-protein interaction (PPI) network of DEGs was constructed using Cytoscape and the key modules were selected from the PPI network based on the MCODE analysis.
  
  
  RESULTS
  A total of 216 DEGs were screened, including 51 up- and 165 down-regulated genes. Meanwhile, nine GO terms were obtained, and DEGs such as SGK1, CRY2 and REV1 were enriched in response to DNA damage stimulus. Furthermore, 89 DEGs and 99 gene pairs were mapped into the PPI network and Ubc was the hob node. Two key modules, which contained the genes (e.g. Ubc, Egr1, Ptgs2, Hmox1, Cd44, Btg2, Cyr61 and Fos) were related to response to DNA damage stimulus.
  
  
  CONCLUSIONS
  The deletion of Hsf4 affects the expression of many genes, such as Ubc, Ptgs2, Egr1 and Fos. These genes may be involved in the development of cataract and could be used as therapeutic targets for cataract.
authors.affiliation: Department of Ophthalmology, Qilu Hospital of Shandong University, Wenhuaxi Road 107, Jinan, Shandong, 250012, China. WenjuanZhaooa@163.com.|Department of Ophthalmology, Shandong University Affiliated Jinan Central Hospital, Jinan, 250013, China. WenjuanZhaooa@163.com.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, The 5th People's Hospital of Jinan, Jinan, 250022, China. wenqingzhaooa@163.com.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Health Examination Center, Jinan 2nd People's Hospital, Jinan, 250001, China. junzhaooa@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Management Science and Engineering, Shandong University of Finance and Economics, Jinan, 250014, China. DongWanggn@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Qilu Hospital of Shandong University, Wenhuaxi Road 107, Jinan, Shandong, 250012, China. jinghailild@163.com.
authors.forename: Jinghai
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002386:Cataract / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D000076249:Heat Shock Transcription Factors; D007908:Lens, Crystalline / Q000378:metabolism; D051379:Mice; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; C484462:Hsf4 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors