title: Permeability Parameters Measured with Dynamic Contrast-Enhanced MRI: Correlation with the Extravasation of Evans Blue in a Rat Model of Transient Cerebral Ischemia.
abstract: OBJECTIVE
  The purpose of this study was to correlate permeability parameters measured with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) using a clinical 3-tesla scanner with extravasation of Evans blue in a rat model with transient cerebral ischemia.
  
  
  METHODS
  Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil. DCE-MRI was performed 12 hours, 18 hours, and 36 hours after reperfusion. Permeability parameters (K(trans), ve, and vp) from DCE-MRI were calculated. Evans blue was injected after DCE-MRI and extravasation of Evans blue was correlated as a reference with the integrity of the blood-brain barrier. Correlation analysis was performed between permeability parameters and the extravasation of Evans blue.
  
  
  RESULTS
  All permeability parameters (K(trans), ve, and vp) showed a linear correlation with extravasation of Evans blue. Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001).
  
  
  CONCLUSIONS
  Permeability parameters obtained by DCE-MRI at 3-T are well-correlated with Evans blue extravasation, and K(trans) shows the strongest correlation among the tested parameters.
authors.affiliation: Department of Radiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Korea.
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Ahn
authors.affiliation: Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Korea.
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Shin
authors.affiliation: Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Korea.
authors.forename: Jinna
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Radiology, College of Medicine, Seoul National University, Seoul 110-744, Korea.
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College of Medicine, Yonsei University, Seoul 120-752, Korea.
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Radiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Neurology, College of Medicine, Yonsei University, Seoul 120-752, Korea.
authors.forename: Ji Hoe
authors.initials: JH
authors.lastname: Heo
authors.affiliation: Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Korea.
authors.forename: Seung-Koo
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology; D002199:Capillary Permeability; D003287:Contrast Media; D004195:Disease Models, Animal; D005070:Evans Blue / Q000032:analysis; D002546:Ischemic Attack, Transient / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000175:diagnosis
chemical_list: D003287:Contrast Media; D005070:Evans Blue
keywords: Animal models; Blood-brain barrier; Brain ischemia; Dynamic contrast-enhanced MRI; Kinetic modeling