title: Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized Stenotrophomonas nitritireducens cells.
abstract: OBJECTIVE
  To improve the production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid (HODA) from α-linolenic acid in Stenotrophomonas nitritireducens.
  
  
  RESULTS
  Cells of the bacterium were permeabilized with 1.25% (v/v) methanol. The optimal conditions for HODA production by permeabilized cells were pH 7, 35 °C, 5% (v/v) DMSO, 50 g cells l(-1), and 22.5 g α-linolenic acid l(-1). Under these conditions, permeabilized cells produced 16.4 g HODA l(-1) after 2 h, with a conversion yield of 73 % (w/w) and a volumetric productivity of 8.2 g l(-1) h(-1). These values were 153 and 230 % of the values for non-permeabilized cells
  
  
  CONCLUSIONS
  This is the highest concentration and volumetric and specific productivities of HODA reported thus far.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Choi
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Seo
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea. deokkun@konkuk.ac.kr.
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001709:Biotechnology; D002463:Cell Membrane Permeability; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D020588:Stenotrophomonas / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000031:analogs & derivatives / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D017962:alpha-Linolenic Acid; C027369:octadecadienoic acid
keywords: 10-Hydroxy-12,15(Z,Z)-octadecadienoic acid; Biotransformation; Cell permeabilization; Stenotrophomonas nitritireducens; α-Linolenic acid