title: Wide-Scope Screening of Illegal Adulterants in Dietary and Herbal Supplements via Rapid Polarity-Switching and Multistage Accurate Mass Confirmation Using an LC-IT/TOF Hybrid Instrument.
abstract: A new analytical strategy was developed that integrates a generic sample preparation into a liquid chromatography-multistage ion trap/time-of-flight mass spectrometry (LC-IT(MS(n))/TOF), allowing for large-scale screening and qualitative confirmation of wide-scope illegal adulterants in different food matrices. Samples were pretreated by a fast single-tube multifunction extraction for accurate multistage mass measurement on the hybrid LC-IT/TOF system. A qualitative validation performed for over 500 analyte-matrix pairs showed the method can reduce most of the matrix effects and achieve a lower limit of confirmation at 0.1 mg/kg for 73% of the target compounds. A unique combination of dual-polarity detection, retention time, isotopic profile, and accurate MS(n) spectra enables more comprehensive and precise confirmation, based on the multiparameter matching by automated library searching against the user-created database. Finally, the applicability of this LC-IT(MS(n))/TOF-based screening procedure for discriminating coeluting isobars, identifying nontarget adulterants, and even tentatively elucidating unexpected species in real samples is demonstrated.
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.affiliation: §Hunan Academy of Inspection and Quarantine and Food Safety Key Laboratory of Hunan Province, Hunan Entry-Exit Inspection and Quarantine Bureau of China, Changsha 410004, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Wang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: §Hunan Academy of Inspection and Quarantine and Food Safety Key Laboratory of Hunan Province, Hunan Entry-Exit Inspection and Quarantine Bureau of China, Changsha 410004, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Dai
authors.affiliation: §Hunan Academy of Inspection and Quarantine and Food Safety Key Laboratory of Hunan Province, Hunan Entry-Exit Inspection and Quarantine Bureau of China, Changsha 410004, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: #Guangzhou Branch, Shimadzu (China) Co., Limited,7FL,T. P. Plaza, 9/109 Liuhua Road, Guangzhou 510010, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Shen
authors.affiliation: ΔNational Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination / Q000331:legislation & jurisprudence / Q000517:prevention & control*; D029001:Herbal Medicine; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: accurate multistage mass spectrum; dietary supplements; hybrid ion trap/time-of-flight mass spectrometry; illegal adulterants; polarity-switching; qualitative screening; single-tube extraction