title: The Functional Study of a Chinese Herbal Compounded Antidepressant Medicine--Jie Yu Chu Fan Capsule on Chronic Unpredictable Mild Stress Mouse Model.
abstract: Jie Yu Chu Fan capsule (JYCF) is a new compounded Chinese herbal medicine for the treatment of depression. The present study was designed to explore the antidepressant effects and the possible mechanisms of JYCF by using chronic unpredictable mild stress (CUMS) mouse model and comparing results to that of fluoxetine. Behavioral tests including an open field test, sucrose preference test and forced swim test were performed to evaluate the antidepressant effects of JYCF. The concentrations of monoamine neurotransmitters and metabolic products including norepinephrine (NE), 5-hydroxytryptamine (5-HT), dopamine (DA), 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in the cerebral cortex and hippocampus of mice were determined by means of high performance liquid chromatography with electrochemical detection (HPLC-EC). The results show that a successful mouse CUMS model was established through 5 weeks of continuous unpredictable stimulation, as indicated by the significant decrease in sucrose preference and locomotor activity and increase in immobility time in the forced swim test. Chronic treatment of JYCF (1.25, 2.5 and 5 g/kg) and fluoxetine (20 mg/kg) significantly reversed the CUMS-induced behavioral abnormalities. JYCF (1.25, 2.5 and 5 g/kg) significantly increased NE in CUMS mouse prefrontal cortex (P < 0.01, P < 0.01, P < 0.05 respectively) and 5-HT in hippocampus (P < 0.05). In summary, our findings suggest that JYCF exerts comparable antidepressant-like effects to that of fluoxetine in CUMS mice. Besides, the antidepressant-like effect of JYCF is mediated by the increase of monoaminergic transmitters including 5-HT and NE.
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, Oklahoma, United States of America.
authors.forename: Teresa
authors.initials: T
authors.lastname: Golden
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, Oklahoma, United States of America.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000627:therapeutic use*; D001522:Behavior, Animal; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002214:Capsules; D002908:Chronic Disease; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005247:Feeding Behavior / Q000187:drug effects; D005473:Fluoxetine / Q000494:pharmacology / Q000627:therapeutic use; D007103:Immobilization; D008297:Male; D008813:Mice, Inbred ICR; D018377:Neurotransmitter Agents / Q000378:metabolism; D013315:Stress, Psychological / Q000188:drug therapy*; D013395:Sucrose / Q000494:pharmacology; D013550:Swimming; D013997:Time Factors
chemical_list: D000928:Antidepressive Agents; D002214:Capsules; D004365:Drugs, Chinese Herbal; D018377:Neurotransmitter Agents; C000607326:jie yu chu fan; D005473:Fluoxetine; D013395:Sucrose