title: Stimulation of the activity of a novel tannase produced in white-rot fungi Phellinus pini, Fomes fomentarius, and Tyromyces pubescens by medium supplementation.
abstract: In recent years, tannase has gained increasing interest mainly because of its potential applications. One of the most important functions of tannic acid (TA) hydrolase is the release of gallic acid (GA) from complex tannins. The aim of the study was to determine the dynamic changes in tannase activity depending on the carbon source in the culture medium. An extracellular and intracellular tannase activity analysis was carried out with the use of spectrophotometric analysis and confirmed by capillary electrophoresis in cultures of white-rot fungi: Phellinus pini, Fomes fomentarius, and Tyromyces pubescens. The inducible potential of TA and rapeseed meal on the activity of tannin acyl hydrolase was confirmed during 14 days of culturing. Different effects of the tested compounds on stimulation of tannase activity in selected fungal strains have been demonstrated. We concluded that rapeseed meal was the best inducer of tannase activity in the case of P. pini. However, the highest concentrations of GA were observed after stimulation by the TA in the cultures of F. fomentarius and T. pubescens.
authors.affiliation: Department of Physiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Prendecka
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland. magdalena.jaszek@poczta.umcs.lublin.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jaszek
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Grąz
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Głuszak
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Małysz
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Nowak
authors.affiliation: Institute of Soil Science and Plant Cultivation, Puławy, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Żuchowski
authors.affiliation: Department of Physiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Małecka-Massalska
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000378:metabolism*; D055453:Coriolaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D005707:Gallic Acid / Q000378:metabolism; D013634:Tannins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D013634:Tannins; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase
keywords: capillary electrophoresis, tannic acid; gallic acid; rapeseed meal; tannase; white-rot fungi