title: Biotransformation of Endocrine-Disrupting Compounds in Groundwater: Bisphenol A, Nonylphenol, Ethynylestradiol and Triclosan by a Laccase Cocktail fromPycnoporus sanguineusCS43.
abstract: The biodegradation of organic compounds present in water at trace concentration has become a critical environmental problem. In particular, enzymatic oxidation by fungal laccases offers a promising alternative for efficient and sustainable removal of organic pollutants in water. In this work, the biocatalytic ability of laccases from thePycnoporus sanguineusCS43 fungus was evaluated. A filtered culture supernatant (laccase cocktail) evidenced an enhanced biotransformation capability to remove common endocrine-disruptor compounds (EDCs), such as bisphenol A, 4-nonylphenol, 17-α-ethynylestradiol and triclosan. A biodegradation of around 89-100 % was achieved for all EDCs using synthetic samples (10 mg L-1) and after the enzymatic treatment with 100 U L-1(50.3 U mg-1). The biodegradation rates obtained were fitted to a first order reaction. Furthermore, enzymatic biocatalytic activity was also evaluated in groundwater samples coming from northwestern Mexico, reaching biotransformation percentages between 55 and 93 % for all tested compounds. As far as we know this is the first study on real groundwater samples in which the enzymatic degradation of target EDCs by a laccase cocktail from any strain ofPycnoporus sanguineuswas evaluated. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided.
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico ; Universidad Juárez Autónoma de Tabasco, Av. Universidad S/N Magisterial, Villahermosa, 86040 Tabasco Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Garcia-Morales
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Delgado
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: K
authors.initials: K
authors.lastname: Gomez-Mariscal
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Orona-Navar
authors.affiliation: Laboratorio de Enzimología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León. Av. Universidad s/n, Ciudad Universitaria San Nicolás de los Garza, San Nicolás de los Garza, NL 64450 Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Hernandez-Luna
authors.affiliation: Posgrado en Ciencias Ambientales, Benemérita Universidad Autónoma de Puebla, Edificio 103G, 7, Puebla, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Torres
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Parra
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: D
authors.initials: D
authors.lastname: Cárdenas-Chávez
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Mahlknecht
authors.affiliation: Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, NL 64849 Mexico.
authors.forename: N
authors.initials: N
authors.lastname: Ornelas-Soto
keywords: Endocrine disrupting compound (EDC); Groundwater; Laccases; Pycnoporus sanguineus