title: Hydrogen sulfide accelerates wound healing in diabetic rats.
abstract: OBJECTIVE
  The aim of this study was to explore the role of hydrogen sulfide on wound healing in diabetic rats.
  
  
  METHODS
  Experimental diabetes in rats was induced by intraperitoneal injection of streptozotocin (STZ) (in 0.1 mol/L citrate buffer, Ph 4.5) at dose of 70 mg/kg. Diabetic and age-matched non-diabetic rats were randomly assigned to three groups: untreated diabetic controls (UDC), treated diabetic administrations (TDA), and non-diabetic controls (NDC). Wound Healing Model was prepared by making a round incision (2.0 cm in diameter) in full thickness. Rats from TDA receive 2% sodium bisulfide ointment on wound, and animals from UDC and NDC receive control cream. After treatment of 21 days with sodium bisulfide, blood samples were collected for determination of vascular endothelial growth factor (VEGF), intercellular cell adhesion molecule-1 (ICAM-1), antioxidant effects. Granulation tissues from the wound were processed for histological examination and analysis of western blot.
  
  
  RESULTS
  The study indicated a significant increase in levels of VEGF and ICAM-1 and a decline in activity of coagulation in diabetic rats treated with sodium bisulfide. Sodium bisulfide treatment raised the activity of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) protein expression, and decreased tumor necrosis factor α (TNF-α) protein expression in diabetic rats.
  
  
  CONCLUSIONS
  The findings in present study suggested that hydrogen sulfide accelerates the wound healing in rats with diabetes. The beneficial effect of H2S may be associated with formation of granulation, anti-inflammation, antioxidant, and the increased level of vascular endothelial growth factor (VEGF).
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Wannan Medical College Wuhu 241002, Anhui, PRC.
authors.forename: Guoguang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Wannan Medical College Wuhu 241002, Anhui, PRC.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: General Hospital of Jinan Military Command Jinan 250031, Shandong, PRC.
authors.forename: Qingying
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Physiology, School of Basic Medicine, Wannan Medical College Wuhu 241002, Anhui, PRC.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Wannan Medical College Wuhu 241002, Anhui, PRC.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Wannan Medical College Wuhu 241002, Anhui, PRC.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000287:Administration, Topical; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D000975:Antioxidants / Q000008:administration & dosage; D001777:Blood Coagulation / Q000187:drug effects; D048909:Diabetes Complications / Q000097:blood / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D006097:Granulation Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000008:administration & dosage* / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009824:Ointments; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013440:Sulfides / Q000008:administration & dosage* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D043925:Angiogenesis Inducing Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D018836:Inflammation Mediators; D009824:Ointments; D013440:Sulfides; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D018799:Intercellular Adhesion Molecule-1; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D013482:Superoxide Dismutase; C025451:sodium bisulfide; D006862:Hydrogen Sulfide
keywords: Hydrogen sulfide; diabetes; wound healing