title: Temperature-Sensitive Salmonella enterica Serovar Enteritidis PT13a Expressing Essential Proteins of Psychrophilic Bacteria.
abstract: Synthetic genes based on deduced amino acid sequences of the NAD-dependent DNA ligase (ligA) and CTP synthetase (pyrG) of psychrophilic bacteria were substituted for their native homologues in the genome of Salmonella enterica serovar Enteritidis phage type 13a (PT13a). The resulting strains were rendered temperature sensitive (TS) and did not revert to temperature resistance at a detectable level. At permissive temperatures, TS strains grew like the parental strain in broth medium and in macrophage-like cells, but their growth was slowed or stopped when they were shifted to a restrictive temperature. When injected into BALB/c mice at the base of the tail, representing a cool site of the body, the strains with restrictive temperatures of 37, 38.5, and 39°C persisted for less than 1 day, 4 to 7 days, and 20 to 28 days, respectively. The wild-type strain persisted at the site of inoculation for at least 28 days. The wild-type strain, but not the TS strains, was also found in spleen-plus-liver homogenates within 1 day of inoculation of the tail and was detectable in these organs for at least 28 days. Intramuscular vaccination of White Leghorn chickens with the PT13a strain carrying the psychrophilic pyrG gene provided some protection against colonization of the reproductive tract and induced an anti-S. enterica antibody response.
authors.affiliation: Duvax Vaccines and Reagents Inc., Victoria, BC, Canada.
authors.forename: Barry N
authors.initials: BN
authors.lastname: Duplantis
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Puckett
authors.affiliation: Zoetis Inc., Kalamazoo, Michigan, USA.
authors.forename: Everett L
authors.initials: EL
authors.lastname: Rosey
authors.affiliation: Zoetis Inc., Kalamazoo, Michigan, USA.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Ameiss
authors.affiliation: Zoetis Inc., Kalamazoo, Michigan, USA.
authors.forename: Angela D
authors.initials: AD
authors.lastname: Hartman
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Pearce
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada fnano@uvic.ca.
authors.forename: Francis E
authors.identifier: http://orcid.org/0000-0002-9363-7830
authors.initials: FE
authors.lastname: Nano
mesh_terms: D034241:Alteromonadaceae / Q000201:enzymology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D011088:DNA Ligases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology*; D031161:Pseudoalteromonas / Q000201:enzymology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D011088:DNA Ligases