title: MiR-302c-3p suppresses invasion and proliferation of glioma cells via down-regulating metadherin (MTDH) expression.
abstract: Glioma is the most common malignant brain tumors with poor prognosis. The molecular events involved in the development and progression of glioma remain unclear. In this study, the expression levels of miR-302c-3p were examined in glioma tissues by qRT-PCR. The in vitro and in vivo functional effects of miR-302c-3p were examined further. Luciferase reporter assays were conducted to confirm the targeting associations. Results showed that the expression level of miR-302c-3p in glioma tissues was significantly lower than those in normal brain tissues (P < 0.001). The decreased expression of mi-302c-3p in glioma was positively associated with WHO grade (P < 0.001). Up-regulation of MTDH was also detected in glioma tumors compared with normal brain tissues (P = 0.0027) and is inversely correlated with miR-302c-3p expression (P = 0.003, R(2) = 0.4065). MTDH mRNA is a direct target of miR-302c-3p, whose ectopic expression decreases MTDH expression through binding to its 3'-untranslated region. Overexpression of miR-302c-3p results in a dramatic inhibition of glioma cells proliferation and invasion in vitro and in vivo. These data suggest that miR-302c-3p play a pivotal role in the progression of glioma by targeting MTDH and is a potential inhibitor in glioma treatment.
authors.affiliation: a Department of Neurosurgery ; First Clinical Medical College of Shanxi Medical University ; Taiyuan , Shanxi Province , China.|b Department of Neurosurgery ; Shanxi Dayi Hospital of Shanxi Medical University ; Taiyuan , Shanxi Province , China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: c Department of Neurosurgery ; Changgung Hospital; Tsinghua University ; Beijing , China.|d Department of Neurosurgery ; Beijing Tiantan Hospital; Capital Medical University ; Beijing , China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wei
authors.affiliation: b Department of Neurosurgery ; Shanxi Dayi Hospital of Shanxi Medical University ; Taiyuan , Shanxi Province , China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Tong
authors.affiliation: b Department of Neurosurgery ; Shanxi Dayi Hospital of Shanxi Medical University ; Taiyuan , Shanxi Province , China.
authors.forename: Laizhao
authors.initials: L
authors.lastname: Chen
authors.affiliation: a Department of Neurosurgery ; First Clinical Medical College of Shanxi Medical University ; Taiyuan , Shanxi Province , China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Fan
authors.affiliation: c Department of Neurosurgery ; Changgung Hospital; Tsinghua University ; Beijing , China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Ji
authors.affiliation: d Department of Neurosurgery ; Beijing Tiantan Hospital; Capital Medical University ; Beijing , China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Jia
authors.affiliation: d Department of Neurosurgery ; Beijing Tiantan Hospital; Capital Medical University ; Beijing , China.
authors.forename: Dongkang
authors.initials: D
authors.lastname: Liu
authors.affiliation: c Department of Neurosurgery ; Changgung Hospital; Tsinghua University ; Beijing , China.|d Department of Neurosurgery ; Beijing Tiantan Hospital; Capital Medical University ; Beijing , China.
authors.forename: Guihuai
authors.initials: G
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D063646:Carcinogenesis; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D034622:RNA Interference; D016601:RNA-Binding Proteins; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; C532169:MIRN302A microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D016601:RNA-Binding Proteins
keywords: MTDH; down-regulation; glioma; inhibition; invasion; microRNA; proliferation