title: Characterization of the Distance Relationship Between Localized Serotonin Receptors and Glia Cells on Fluorescence Microscopy Images of Brain Tissue.
abstract: We here present two new methods for the characterization of fluorescent localization microscopy images obtained from immunostained brain tissue sections. Direct stochastic optical reconstruction microscopy images of 5-HT1A serotonin receptors and glial fibrillary acidic proteins in healthy cryopreserved brain tissues are analyzed. In detail, we here present two image processing methods for characterizing differences in receptor distribution on glial cells and their distribution on neural cells: One variant relies on skeleton extraction and adaptive thresholding, the other on k-means based discrete layer segmentation. Experimental results show that both methods can be applied for distinguishing classes of images with respect to serotonin receptor distribution. Quantification of nanoscopic changes in relative protein expression on particular cell types can be used to analyze degeneration in tissues caused by diseases or medical treatment.
authors.affiliation: 1Department of Medical Engineering,University of Applied Sciences Upper Austria,Garnisonstraße 21,4020 Linz,Austria.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Jacak
authors.affiliation: 3Bioinformatics Research Group,University of Applied Sciences Upper Austria,Softwarepark 11,4232 Hagenberg,Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Schaller
authors.affiliation: 3Bioinformatics Research Group,University of Applied Sciences Upper Austria,Softwarepark 11,4232 Hagenberg,Austria.
authors.forename: Daniela
authors.initials: D
authors.lastname: Borgmann
authors.affiliation: 3Bioinformatics Research Group,University of Applied Sciences Upper Austria,Softwarepark 11,4232 Hagenberg,Austria.
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Winkler
mesh_terms: D001921:Brain / Q000166:cytology*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009457:Neuroglia / Q000737:chemistry*; D011985:Receptors, Serotonin / Q000032:analysis*
chemical_list: D011985:Receptors, Serotonin
keywords: brain tissue; clustering; image processing; serotonin receptors; spatial relationship; super-resolution microscopy