title: Microbial characterization of anode-respiring bacteria within biofilms developed from cultures previously enriched in dissimilatory metal-reducing bacteria.
abstract: This work evaluated the use of a culture enriched in DMRB as a strategy to enrich ARB on anodes. DMRB were enriched with Fe(III) as final electron acceptor and then transferred to a potentiostatically-controlled system with an anode as sole final electron acceptor. Three successive iron-enrichment cultures were carried out. The first step of enrichment revealed a successful selection of the high current-producing ARB Geoalkalibacter subterraneus. After few successive enrichment steps, the microbial community analysis in electroactive biofilms showed a significant divergence with an impact on the biofilm electroactivity. Enrichment of ARB in electroactive biofilms through the pre-selection of DMRB should therefore be carefully considered.
authors.affiliation: INRA, UR0050, Laboratoire de Biotechnologie de l'Environnement (LBE), avenue des Etangs, F-11100 Narbonne, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Pierra
authors.affiliation: INRA, UR0050, Laboratoire de Biotechnologie de l'Environnement (LBE), avenue des Etangs, F-11100 Narbonne, France.
authors.forename: Alessandro A
authors.initials: AA
authors.lastname: Carmona-Martínez
authors.affiliation: INRA, UR0050, Laboratoire de Biotechnologie de l'Environnement (LBE), avenue des Etangs, F-11100 Narbonne, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Trably
authors.affiliation: INRA, UR0050, Laboratoire de Biotechnologie de l'Environnement (LBE), avenue des Etangs, F-11100 Narbonne, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
authors.affiliation: INRA, UR0050, Laboratoire de Biotechnologie de l'Environnement (LBE), avenue des Etangs, F-11100 Narbonne, France. Electronic address: nicolas.bernet@supagro.inra.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bernet
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D019069:Cell Respiration; D004560:Electricity; D004566:Electrodes; D008670:Metals / Q000378:metabolism*; D010084:Oxidation-Reduction; D025341:Principal Component Analysis
chemical_list: D008670:Metals
keywords: Anode respiring bacteria; Dissimilatory metal-reducing bacteria; Geoalkalibacter subterraneus; Microbial bioelectrochemical systems