title: [Continuous cell subline A4C2/9K and its application to the african swine fever virus study].
abstract: A new continuous cell subline A4C2/9K highly sensitive to the african swine fever virus (ASFV) was prepared. All the tested ASFV strains isolated in the Russian Federation in 2008-2013 proliferated in this cell culture exhibiting hemadsorption and accumulated at a titer of up to 6.5 Ig HAU50/cm3. The cell culture A4C2/9K can be used for ASFV isolation or determination of its infectious activity and serotype identity. The culture versions of the ASFV strain Stavropol 01/08 at passages 24 and 33 in the cell culture A4C2/9K lost their pathogenicity for pigs.
authors.forename: V I
authors.initials: VI
authors.lastname: Balysheva
authors.forename: E Yu
authors.initials: EY
authors.lastname: Prudnikova
authors.forename: T V
authors.initials: TV
authors.lastname: Galnbek
authors.forename: V M
authors.initials: VM
authors.lastname: Balyshev
mesh_terms: D000357:African Swine Fever / Q000276:immunology*; D000358:African Swine Fever Virus / Q000254:growth & development* / Q000276:immunology* / Q000472:pathogenicity*; D000818:Animals; D002460:Cell Line / Q000166:cytology / Q000821:virology*; D012426:Russia; D013552:Swine