title: Matrices of a hydrophobically functionalized hyaluronic acid derivative for the locoregional tumour treatment.
abstract: A hyaluronic acid (HA) derivative bearing octadecylamine and acylhydrazine functionalities has been here employed for the production of a paclitaxel delivering matrix for locoregional chemotherapy. Through a strategy consisting in a powder compression and a plasticization with a mixture water/ethanol, a physically assembled biomaterial, stable in solutions with physiologic ionic strengths, has been produced. Two different drug loading strategies have been adopted, by using paclitaxel as chemotherapic agent, and obtained samples have been assayed in terms of release in enhanced solubility conditions and in vitro and in vivo tumoural cytotoxicity. In particular sample with the best releasing characteristics was chosen for an in vivo evaluation against a HCT-116 xenograft on mice. Local tumour establishment and metastatic diffusion was assayed locally at the site of xenograft implantation and at the tributary lymph nodes. Obtained results demonstrated how loading procedure influenced paclitaxel crystallinity into the matrix and consequently drug diffusion and its cytoreductive potential. Chosen paclitaxel loaded matrix was able to drastically inhibit HCT-116 establishment and metastatic diffusion.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: fabiosalvatore.palumbo@unipa.it.
authors.forename: Fabio Salvatore
authors.initials: FS
authors.lastname: Palumbo
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Histopathology and Immunohistochemistry Laboratory, Palermo, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Puleio
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Calogero
authors.initials: C
authors.lastname: Fiorica
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy; IBIM-CNR, Via Ugo La Malfa 153, 90146 Palermo, Italy; Institute of Biophysics at Palermo, Italian National Research Council, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pitarresi
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Histopathology and Immunohistochemistry Laboratory, Palermo, Italy.
authors.forename: Guido Ruggero
authors.initials: GR
authors.lastname: Loria
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Histopathology and Immunohistochemistry Laboratory, Palermo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cassata
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy; IBIM-CNR, Via Ugo La Malfa 153, 90146 Palermo, Italy; Institute of Biophysics at Palermo, Italian National Research Council, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use*; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D009994:Osmolar Concentration; D017239:Paclitaxel / Q000494:pharmacology; D040521:Subcutaneous Tissue / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; D017239:Paclitaxel
keywords: Biomaterials; Hyaluronic acid; Locoregional tumour treatment; Paclitaxel