title: Selective propensity of bovine jugular vein material to bacterial adhesions: An in-vitro study.
abstract: BACKGROUND
  Percutaneous pulmonary valve implantation (PPVI) using Melody valve made of bovine jugular vein is safe and effective. However, infective endocarditis has been reported for unclear reasons. We sought to assess the impact of valvular substrates on selective bacterial adhesion.
  
  
  METHODS
  Three valved stents (Melody valve, homemade stents with bovine and porcine pericardium) were tested in-vitro for bacterial adhesion using Staphylococcus aureus and Streptococcus sanguinis strains.
  
  
  RESULTS
  Bacterial adhesion was higher on bovine jugular venous wall for S. aureus and on Melody valvular leaflets for S. sanguinis in control groups and significantly increased in traumatized Melody valvular leaflets with both bacteria (traumatized vs non traumatized: p=0.05). Bacterial adhesion was lower on bovine pericardial leaflets.
  
  
  CONCLUSIONS
  Selective adhesion of S. aureus and S. sanguinis pathogenic strains to Melody valve tissue was noted on healthy tissue and increased after implantation procedural steps.
authors.affiliation: Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France.
authors.forename: Zakaria
authors.initials: Z
authors.lastname: Jalal
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris, France.
authors.forename: Louise
authors.initials: L
authors.lastname: Galmiche
authors.affiliation: Hôpital Necker Enfants Malades, Laboratoire d'Anatomopathologie, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Lebeaux
authors.affiliation: Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Villemain
authors.affiliation: Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France.
authors.forename: Georgia
authors.initials: G
authors.lastname: Brugada
authors.affiliation: Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France.
authors.forename: Mehul
authors.initials: M
authors.lastname: Patel
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Ghigo
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Beloin
authors.affiliation: Centre de Référence Malformations Cardiaques Congénitales Complexes - M3C, Necker Hospital for Sick Children, Assistance Publique des Hôpitaux de Paris, Pediatric Cardiology, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France. Electronic address: younes.boudjemline@nck.aphp.fr.
authors.forename: Younes
authors.initials: Y
authors.lastname: Boudjemline
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001705:Bioprosthesis / Q000382:microbiology*; D002417:Cattle; D006350:Heart Valve Prosthesis / Q000382:microbiology*; D019918:Heart Valve Prosthesis Implantation / Q000009:adverse effects*; D006801:Humans; D007601:Jugular Veins / Q000382:microbiology* / Q000637:transplantation; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013298:Streptococcus sanguis / Q000302:isolation & purification; D013552:Swine
keywords: Endocarditis; Fallot; Melody valve; Transcatheter pulmonary valve