title: Bacteria Murmur: Application of an Acoustic Biosensor for Plant Pathogen Detection.
abstract: A multi-targeting protocol for the detection of three of the most important bacterial phytopathogens, based on their scientific and economic importance, was developed using an acoustic biosensor (the Quartz Crystal Microbalance) for DNA detection. Acoustic detection was based on a novel approach where DNA amplicons were monitored and discriminated based on their length rather than mass. Experiments were performed during real time monitoring of analyte binding and in a direct manner, i.e. without the use of labels for enhancing signal transduction. The proposed protocol improves time processing by circumventing gel electrophoresis and can be incorporated as a routine detection method in a diagnostic lab or an automated lab-on-a-chip system for plant pathogen diagnostics.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Heraklion Crete, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Papadakis
authors.affiliation: Benaki Phytopathological Institute, 8 St. Delta, Kifissia, Athens, Greece.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Skandalis
authors.affiliation: Benaki Phytopathological Institute, 8 St. Delta, Kifissia, Athens, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Dimopoulou
authors.affiliation: Benaki Phytopathological Institute, 8 St. Delta, Kifissia, Athens, Greece.
authors.forename: Paraskevas
authors.initials: P
authors.lastname: Glynos
authors.affiliation: Institute of Molecular Biology and Biotechnology, Heraklion Crete, Greece; Dept. of Biology, University of Crete, Vassilika Vouton, Heraklion Crete, Greece.
authors.forename: Electra
authors.initials: E
authors.lastname: Gizeli
mesh_terms: D000162:Acoustics; D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D057230:Limit of Detection; D018551:Solanum lycopersicum / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction