title: Composition and in vitro cytotoxic activities of essential oil of Hedychium spicatum from different geographical regions of western Himalaya by principal components analysis.
abstract: The rhizome of Hedychium spicatum has been widely used in traditional medicines. The present study deals with the evaluation of the cytotoxic potential of rhizome essential oils from four different regions of the Western Himalaya (India) along with comparative correlation analysis to characterise the bioactive cytotoxic component. The essential oils were coded as MHS-1, MHS-2, MHS-3 and MHS-4, and characterised using GC-FID and GC-MS. The main volatile compounds identified were 1,8-cineol, eudesmol, cubenol, spathulenol and α-cadinol. In vitro cytotoxic activities were assessed against human cancer cell lines such as, the lung (A549), colon (DLD-1, SW 620), breast (MCF-7, MDA-MB-231), head and neck (FaDu), and cervix (HeLa). MHS-4 is significantly active in comparison to other samples against all cancer cell lines. Sample MHS-4 has major proportion of monoterpene alcohol mainly 1,8-cineol. Principal components analysis was performed for the experimental results and all four samples were clustered according to their percentage inhibition at different doses.
authors.affiliation: a Phytochemistry Division, CSIR-National Botanical Research Institute , Lucknow 226 001 , India.
authors.forename: Tripti
authors.initials: T
authors.lastname: Mishra
authors.affiliation: a Phytochemistry Division, CSIR-National Botanical Research Institute , Lucknow 226 001 , India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Pal
authors.affiliation: b Biochemistry Division, CSIR-Central Drug Research Institute , Janki Puram Extension, Sitapur Road, Lucknow 226031 , India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Meena
authors.affiliation: b Biochemistry Division, CSIR-Central Drug Research Institute , Janki Puram Extension, Sitapur Road, Lucknow 226031 , India.
authors.forename: Dipak
authors.initials: D
authors.lastname: Datta
authors.affiliation: a Phytochemistry Division, CSIR-National Botanical Research Institute , Lucknow 226 001 , India.
authors.forename: Prateek
authors.initials: P
authors.lastname: Dixit
authors.affiliation: a Phytochemistry Division, CSIR-National Botanical Research Institute , Lucknow 226 001 , India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.affiliation: c Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute , Lucknow 226001 , India.
authors.forename: Baleshwar
authors.initials: B
authors.lastname: Meena
authors.affiliation: c Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute , Lucknow 226001 , India.
authors.forename: T S
authors.initials: TS
authors.lastname: Rana
authors.affiliation: c Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute , Lucknow 226001 , India.
authors.forename: D K
authors.initials: DK
authors.lastname: Upreti
mesh_terms: D045744:Cell Line, Tumor; D003511:Cyclohexanols / Q000737:chemistry / Q000302:isolation & purification; D000077591:Eucalyptol; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007194:India; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D025341:Principal Component Analysis; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification; D045787:Sesquiterpenes, Eudesmane / Q000737:chemistry / Q000302:isolation & purification; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; D013729:Terpenes; C445364:cadinol; C445365:cubenol; C051082:beta-eudesmol; C013258:spathulenol; D000077591:Eucalyptol
keywords: H. spicatum; cytotoxic activity; principal components analysis; rhizome essential oil