title: Lab in a Tube: Sensitive Detection of MicroRNAs in Urine Samples from Bladder Cancer Patients Using a Single-Label DNA Probe with AIEgens.
abstract: We demonstrate an ultrasensitive microRNA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. Furthermore, the assay could accomplish various detection limits by tuning the reaction temperature due to the different activity of exonuclease III corresponding to the diverse temperature. Specifically, 1 pM miR-21 can be detected in 40 min at 37 °C, and 10 aM (about 300 molecules in 50 μL) miR-21 could be discriminated in 7 days at 4 °C. The great specificity of the assay guarantees that the real 21 urine samples from the bladder cancer patients are successfully detected by our method.
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Min
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Jia
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Abdul
authors.initials: A
authors.lastname: Hakeem
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Fuxin
authors.initials: F
authors.lastname: Zheng
authors.affiliation: ‡National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: §Department of Chemistry, HKUST Jockey Club Institute for Advanced Study Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Xiaoding
authors.initials: X
authors.lastname: Lou
authors.affiliation: †Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.|‡National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xia
mesh_terms: D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000652:urine*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D035683:MicroRNAs
keywords: AIEgens; DNA; exonuclease III; miR-21; urine samples