title: Arrival time distributions of product ions reveal isomeric ratio of deprotonated molecules in ion mobility-mass spectrometry of hyaluronan-derived oligosaccharides.
abstract: Hyaluronic acid is a naturally occurring linear polysaccharide with substantial medical potential. In this work, discrimination of tyramine-based hyaluronan derivatives was accessed by ion mobility-mass spectrometry of deprotonated molecules and nuclear magnetic resonance spectroscopy. As the product ion mass spectra did not allow for direct isomer discrimination in mixture, the reductive labeling of oligosaccharides as well as stable isotope labeling was performed. The ion mobility separation of parent ions together with the characteristic fragmentation for reduced isomers providing unique product ions allowed us to identify isomers present in a mixture and determine their mutual isomeric ratio. The determination used simple recalculation of arrival time distribution areas of unique ions to areas of deprotonated molecules. Mass spectrometry data were confirmed by nuclear magnetic resonance spectroscopy.
authors.affiliation: Contipro Pharma, a.s., 561 02 Dolní Dobrouč, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Hermannová
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, Olomouc, 771 46, Czech Republic.
authors.forename: Andreea-Maria
authors.initials: AM
authors.lastname: Iordache
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, Olomouc, 771 46, Czech Republic.
authors.forename: Kristína
authors.initials: K
authors.lastname: Slováková
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, Olomouc, 771 46, Czech Republic.|Institute of Microbiology, ASCR, v.v.i., Videnska 1083, 142 20, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Havlíček
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, Olomouc, 771 46, Czech Republic.|Institute of Microbiology, ASCR, v.v.i., Videnska 1083, 142 20, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Pelantová
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, Olomouc, 771 46, Czech Republic.|Institute of Microbiology, ASCR, v.v.i., Videnska 1083, 142 20, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
mesh_terms: D006820:Hyaluronic Acid / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D007477:Ions; D009844:Oligosaccharides; D006820:Hyaluronic Acid
keywords: ion mobility; isomer discrimination; mass spectrometry; nuclear magnetic resonance spectroscopy; tyramine-based hyaluronan derivatives