title: The Research of No-Touch Isolation Technique on the Prevention of Postoperative Recurrence and Metastasis of Hepatocellular Carcinoma after Hepatectomy.
abstract: OBJECTIVE
  To evaluate the efficacy of a surgical no-touch isolation technique in primary hepatocellular carcinoma patients compared with traditional hepatectomy.
  
  
  METHODS
  Eighty hepatocellular carcinoma patients were randomly divided into traditional hepatectomy (n = 40) and no-touch isolation technique groups (n = 40). We compared peripheral blood variations in alpha-fetoprotein mRNA, miRNA-221, miRNA-224, and miRNA-122 expression levels in both groups pre- and postoperatively using real-time fluorescent quantitative polymerase chain reaction.
  
  
  RESULTS
  The patients in the no-touch isolation technique group had better clinical curative effect. In the traditional hepatectomy group, variations in alpha-fetoprotein mRNA copy number pre- and postoperatively indirectly indicated a significant increase in the number of exfoliated carcinoma cells induced by manipulating the liver, increasing the risk of postoperative recurrence and metastasis (P < 0.05). Traditional hepatectomy patients showed higher increases in miRNA-221 and miRNA-224 expression than those in the no-touch isolation technique group (P < 0.05). Tumor resection resulted in preoperative expression and high postoperative expression of miRNA-122. No-touch isolation technique patients showed a slight and significant increase (P < 0.01) in miRNA-122, which was positively correlated with postoperative liver function index. Conclusion: The no-touch isolation technique is more effective than traditional hepa- tectomy in tumor resection for primary hepatocellular carcinoma.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Ke Sen
authors.initials: KS
authors.lastname: Xu
authors.forename: Jian Sheng
authors.initials: JS
authors.lastname: Li
authors.forename: Wei Dong
authors.initials: WD
authors.lastname: Jia
authors.forename: Wen Bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: He
authors.forename: Ge Liang
authors.initials: GL
authors.lastname: Xu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics / Q000401:mortality / Q000556:secondary* / Q000601:surgery*; D002681:China; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods* / Q000401:mortality; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics / Q000401:mortality / Q000473:pathology* / Q000601:surgery*; D008297:Male; D035683:MicroRNAs / Q000097:blood; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000097:blood / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000097:blood; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; C097353:AFP protein, human; C531907:MIRN122 microRNA, human; C525709:MIR221, human; C546140:MIRN224 microRNA, human