title: Resveratrol Derivative-Rich Melinjo Seed Extract Attenuates Skin Atrophy in Sod1-Deficient Mice.
abstract: The oxidative damages induced by a redox imbalance cause age-related changes in cells and tissues. Superoxide dismutase (SOD) enzymes play a pivotal role in the antioxidant system and they also catalyze superoxide radicals. Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of murine tissue, SOD1 is essential for the maintenance of tissue homeostasis. Melinjo (Gnetum gnemon Linn) seed extract (MSE) contains trans-resveratrol (RSV) and resveratrol derivatives, including gnetin C, gnemonoside A, and gnemonoside D. MSE intake also exerts no adverse events in human study. In the present studies, we investigated protective effects of MSE on age-related skin pathologies in mice. Orally MSE and RSV treatment reversed the skin thinning associated with increased oxidative damage in the Sod1 (-/-) mice. Furthermore, MSE and RSV normalized gene expression of Col1a1 and p53 and upregulated gene expression of Sirt1 in skin tissues. In vitro experiments revealed that RSV significantly promoted the viability of Sod1 (-/-) fibroblasts. These finding demonstrated that RSV in MSE stably suppressed an intrinsic superoxide generation in vivo and in vitro leading to protecting skin damages. RSV derivative-rich MSE may be a powerful food of treatment for age-related skin diseases caused by oxidative damages.
authors.affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shibuya
authors.affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ozawa
authors.affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Izuo
authors.affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D035684:Gnetum / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010936:Plant Extracts / Q000737:chemistry; D000077185:Resveratrol; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013267:Stilbenes / Q000737:chemistry / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics*; D000072105:Superoxide Dismutase-1; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D001572:Benzofurans; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D010936:Plant Extracts; C000606290:SOD1 protein, human; D013267:Stilbenes; D016159:Tumor Suppressor Protein p53; C584041:gnemonoside A; C584042:gnemonoside D; C575740:gnetin C; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D000077185:Resveratrol