title: Protective Effect of Lycium ruthenicum Murr. Against Radiation Injury in Mice.
abstract: The protective effect of Lycium ruthenicum Murr. against radiation injury was examined in mice. Kunming mice were randomly divided into a control group, model group, positive drug group and L. ruthenicum high dose (8 g/kg), L. ruthenicum middle dose (4 g/kg), L. ruthenicum low dose (2 g/kg) treatment groups, for which doses were administered the third day, seventh day and 14th day after irradiation. L. ruthenicum extract was administered orally to the mice in the three treatment groups and normal saline was administered orally to the mice in the control group and model group for 14 days. The positive group was treated with amifostine (WR-2721) at 30 min before irradiation. Except for the control group, the groups of mice received a 5 Gy quantity of X-radiation evenly over their whole body at one time. Body weight, hemogram, thymus and spleen index, DNA, caspase-3, caspase-6, and P53 contents were observed at the third day, seventh day, and 14th day after irradiation. L. ruthenicum could significantly increase the total red blood cell count, hemoglobin count and DNA contents (p < 0.05). The spleen index recovered significantly by the third day and 14th day after irradiation (p < 0.05). L. ruthenicum low dose group showed a significant reduction in caspase-3 and caspase-6 of serum in mice at the third day, seventh day, and 14th day after irradiation and L. ruthenicum middle dose group experienced a reduction in caspase-6 of serum in mice by the seventh day after irradiation. L. ruthenicum could decrease the expression of P53. The results showed that L. ruthenicum had protective effects against radiation injury in mice.
authors.affiliation: Department of Pharmacy, Qinghai University Medical College, Xining 810001, China. ddpatentdd@163.com.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Department of Radiotherapy Oncology, Qinghai University Affiliated Hospital, Xining 810001, China. chfa1964@126.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Qinghai University Medical College, Xining 810001, China. yaoxingcheng0625@163.com.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Pharmacy, Qinghai University Medical College, Xining 810001, China. qhmczjb@163.com.
authors.forename: Junbo
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Radiotherapy Oncology, Qinghai University Affiliated Hospital, Xining 810001, China. jiayun_660@163.com.
authors.forename: Cai
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Qinghai University Medical College, Xining 810001, China. qhmczjb@163.com.
authors.forename: Juanling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Qinghai University Medical College, Xining 810001, China. qhmclxy@163.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000097:blood; D053178:Caspase 6 / Q000097:blood; D032305:Lycium / Q000737:chemistry*; D051379:Mice; D008517:Phytotherapy; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014916:Whole-Body Irradiation; D014965:X-Rays
chemical_list: D011837:Radiation-Protective Agents; D016159:Tumor Suppressor Protein p53; C505409:Casp3 protein, mouse; C505516:Casp6 protein, mouse; D053148:Caspase 3; D053178:Caspase 6
keywords: Lycium ruthenicum Murr.; apoptosis; hematopoietic; radioprotection