title: Chitosan multiple addition enhances laccase production from Trametes versicolor.
abstract: Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L(-1) chitosan to a 2-day-old culture media, with 24-h interval between the treatments. Under these conditions, laccase activity of 644.9 U l(-1) was achieved on the seventh day and laccase production was improved by 93.5 % higher than the control. Chitosan treatment increased reactive oxygen species generation and extracellular protein concentration in the treated mycelia. In contrast, the inducer inhibited the mycelia growth. The result of the quantitative reverse transcription polymerase chain reaction showed that the copy number of the laccase gene transcript increased by 16.7-fold in the treated mycelia relative to the control. This study provides insight into some of the intrinsic metabolic processes involved in the upregulation of laccase production in the presence of chitosan inducer in fungal culture.
authors.affiliation: National Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Abiodun Emmanuel
authors.initials: AE
authors.lastname: Adekunle
authors.affiliation: National Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hu
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China.
authors.forename: Anzhou
authors.initials: A
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People's Republic of China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
authors.affiliation: National Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China. czliu@home.ipe.ac.cn.
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Liu
mesh_terms: D048271:Chitosan / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055454:Trametes / Q000187:drug effects* / Q000201:enzymology*
chemical_list: D017382:Reactive Oxygen Species; D048271:Chitosan; D042845:Laccase
keywords: Chitosan; Laccase; Multiple addition; Mycelia; Trametes versicolor