title: Deep sequencing and analysis of small RNAs in sweet orange grafted on sour orange infected with two citrus tristeza virus isolates prevalent in Sicily.
abstract: Two representative isolates of a citrus tristeza virus population in Sicily, SG29 (aggressive) and Bau282 (mild), were sequenced via viral small RNAs (vsRNA) produced in budlings of sweet orange grafted on sour orange. Phylogenetic relationships with Mediterranean and exotic isolates revealed that SG29 clustered within the "VT-Asian" subtype, whereas Bau282 belonged to the cluster T30. The study confirms that molecular data need to be integrated with bio-indexing in order to obtain adequate information for risk assessment.
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy. grazialicci@tiscali.it.
authors.forename: Grazia
authors.initials: G
authors.lastname: Licciardello
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scuderi
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Ferraro
authors.affiliation: CNR, Institute for Sustainable Plant Protection, Via Amendola 165/A, 70126, Bari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Giampetruzzi
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Russo
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lombardo
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Raspagliesi
authors.affiliation: Gimlaotec, 8 Hazanchanim St., Rehovot, 76211, Israel.
authors.forename: Moshe
authors.initials: M
authors.lastname: Bar-Joseph
authors.affiliation: Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str. le Lancia 57, 95121, Catania, Italy.
authors.forename: Antonino
authors.initials: A
authors.lastname: Catara
mesh_terms: D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012802:Sicily; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins