title: In vitrostudy on shRNA-mediated reduction of testis developmental related gene 1 expression and its effects on the proliferation, invasion and apoptosis of NTERA-2 cells.
abstract: Testis developmental related gene 1 (TDRG1) is a novel human testis-specific gene.TDRG1is differentially expressed in cancerous tissue compared with normal testicular tissue and demonstrates a unique expression pattern in normal testes; therefore, this gene may be involved in the occurrence and development of testicular germ cell tumors (TGCT). In the present study, the expression level ofTDRG1was downregulated in human TGCT NTERA-2 cells by RNA interference (RNAi) in order to investigate the association between TDRG1 and TGCT. The TDRG1 mRNA and protein expression levels in NTERA-2 cells were significantly inhibited following transfection with specific RNAi plasmids. The ability to proliferate (inhibited by 15.4% at day 3 and 26.1% at day 5; P<0.001) and invade (reduced by 49.1%; P=0.01)in vitrowas suppressed in cells in which the expression level ofTDRG1was reduced, and a corresponding increase in the apoptotic potential was observed (the early apoptotic potential and total apoptotic potential were increased by 75%; P=0.019 and 54.8%; P=0.009, respectively). The results of the present study indicated that the biological behavior of NTERA-2 cells is associated withTDRG1expression levels, and that this gene may be a novel target candidate in the treatment of TGCT.
authors.affiliation: Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Y U
authors.initials: YU
authors.lastname: Gan
authors.affiliation: Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jianfu
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Tang
keywords: NTERA-2; TDRG1; cell biological behavior; in vitro; siRNA