title: The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors.
abstract: Glioblastoma multiforme (GBM) is the most common and deadliest primary brain tumor, driving patients to death within 15 months after diagnosis (short term survivors, ST), with the exception of a small fraction of patients (long term survivors, LT) surviving longer than 36 months. Here we present deep sequencing data showing that peritumoral (P) areas differ from healthy white matter, but share with their respective frankly tumoral (C) samples, a number of mRNAs and microRNAs representative of extracellular matrix remodeling, TGFβ and signaling, of the involvement of cell types different from tumor cells but contributing to tumor growth, such as microglia or reactive astrocytes. Moreover, we provide evidence about RNAs differentially expressed in ST vs LT samples, suggesting the contribution of TGF-β signaling in this distinction too. We also show that the edited form of miR-376c-3p is reduced in C vs P samples and in ST tumors compared to LT ones. As a whole, our study provides new insights into the still puzzling distinction between ST and LT tumors, and sheds new light onto that "grey" zone represented by the area surrounding the tumor, which we show to be characterized by the expression of several molecules shared with the proper tumor mass.
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Fazi
authors.affiliation: CNR, Institute of Cell Biology and Neurobiology, Rome, Italy.|Genomnia srl, Lainate, Milan, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: Felsani
authors.affiliation: Department of Physics, University of Rome "La Sapienza", Rome, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Grassi
authors.affiliation: CNR, Institute of Cell Biology and Neurobiology, Rome, Italy.|Genomnia srl, Lainate, Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Moles
authors.affiliation: Department of Physics, University of Rome "La Sapienza", Rome, Italy.
authors.forename: Daniel
authors.initials: D
authors.lastname: D'Andrea
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy.|Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, USA.|Harvard Medical School, Boston, MA, USA.
authors.forename: Nicola
authors.initials: N
authors.lastname: Toschi
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Daria
authors.initials: D
authors.lastname: Sicari
authors.affiliation: Department of Head and Neck, Institute of Neurosurgery, Catholic University of Sacred Heart, Rome, Italy.|Neurosurgery, Ferrara University Hospital S. Anna, Cona di Ferrara, Ferrara, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: De Bonis
authors.affiliation: Department of Head and Neck, Institute of Neurosurgery, Catholic University of Sacred Heart, Rome, Italy.
authors.forename: Carmelo
authors.initials: C
authors.lastname: Anile
authors.affiliation: Department of Physics, University of Rome "La Sapienza", Rome, Italy.
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Guerrisi
authors.affiliation: Institute of Anatomic Pathology, University Hospital "A. Gemelli", Catholic University of Sacred Heart, Rome, Italy.
authors.forename: Emilia
authors.initials: E
authors.lastname: Luca
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Farace
authors.affiliation: Department of Head and Neck, Institute of Neurosurgery, Catholic University of Sacred Heart, Rome, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Maira
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Silvia Anna
authors.initials: SA
authors.lastname: Ciafré
authors.affiliation: Department of Head and Neck, Institute of Neurosurgery, Catholic University of Sacred Heart, Rome, Italy.
authors.forename: Annunziato
authors.initials: A
authors.lastname: Mangiola
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction; D059467:Transcriptome
keywords: TGFβ; editing; glioblastoma; microRNA; peritumoral area