title: Overexpression of NDC80 is correlated with prognosis of pancreatic cancer and regulates cell proliferation.
abstract: OBJECTIVE
  NDC80/Hec1, one of four proteins of the outer kinetochore NDC80 complex, is involved in the tumorigenesis of a variety of cancers. In this study, we focused on that NDC80 is overexpressed in human pancreatic cancer and investigates the role of NDC80-knockdown in pancreatic cancer cells proliferation.
  
  
  METHODS
  We determined the expression levels of NDC80 on both mRNA and protein levels in fresh pancreatic cancer tissues and cells by quantitative real-time polymerase chain reaction and immunoblotting, respectively. Furthermore, protein level of NDC80 was identified using immunochemistry in paraffin-embedded tumor specimen, with correlation between NDC80 expression and various clinicopathological parameters evaluated. The role of NDC80 in pancreatic cancer cells (Panc-1) growth was investigated by lentivirus-mediated silencing of NDC80. The effect of NDC80 deletion on cell proliferation was analyzed by MTT assay and clone formation assay, while cell cycle distributions and apoptosis were analyzed by flow cytometry.
  
  
  RESULTS
  The mRNA and protein of NDC80 were overexpressed in pancreatic cancer tissues and cells. The statistical analysis based on immunohistochemical evaluation suggested that NDC80 overexpression was signifi cantly associated with clinicopathological parameters including pathological T staging and N staging, which may be served as an predictor for poor outcomes. The silencing of NDC80 in Panc-1 cells could suppress cell proliferation and colony formation. Furthermore, the NDC80-siRNA infected Panc-1 cells lead to cell cycle arrest at G2/M phase and induction of apoptosis.
  
  
  CONCLUSIONS
  These results demonstrated that NDC80 plays an essential role in the tumorigenesis of pancreatic cancer, and might serve as potential prognostic and therapeutic target for treatment of pancreatic cancer.
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Qing-Cai
authors.initials: QC
authors.lastname: Meng
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Hong-Cheng
authors.initials: HC
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Zi-Liang
authors.initials: ZL
authors.lastname: Song
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Ze-Zhi
authors.initials: ZZ
authors.lastname: Shan
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University 600 Yi-Shan Road, Shanghai 200233, China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Huang
keywords: NDC80; apoptosis; pancreatic cancer; proliferation