title: Structure of the external aldimine form of PglE, an aminotransferase required for N,N'-diacetylbacillosamine biosynthesis.
abstract: N,N'-diacetylbacillosamine is a novel sugar that plays a key role in bacterial glycosylation. Three enzymes are required for its biosynthesis in Campylobacter jejuni starting from UDP-GlcNAc. The focus of this investigation, PglE, catalyzes the second step in the pathway. It is a PLP-dependent aminotransferase that converts UDP-2-acetamido-4-keto-2,4,6-trideoxy-d-glucose to UDP-2-acetamido-4-amino-2,4,6-trideoxy-d-glucose. For this investigation, the structure of PglE in complex with an external aldimine was determined to a nominal resolution of 2.0 Å. A comparison of its structure with those of other sugar aminotransferases reveals a remarkable difference in the manner by which PglE accommodates its nucleotide-linked sugar substrate.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin, 53706.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Riegert
authors.affiliation: Human Health Therapeutics, National Research Council Canada, Ottawa, Ontario, Canada, K1A OR6.
authors.forename: N Martin
authors.initials: NM
authors.lastname: Young
authors.affiliation: Human Health Therapeutics, National Research Council Canada, Ottawa, Ontario, Canada, K1A OR6.
authors.forename: David C
authors.initials: DC
authors.lastname: Watson
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin, 53706.
authors.forename: James B
authors.initials: JB
authors.lastname: Thoden
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin, 53706.
authors.forename: Hazel M
authors.initials: HM
authors.lastname: Holden
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000201:enzymology; D002240:Carbohydrate Sequence; D018360:Crystallography, X-Ray; D004072:Digitonin / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D012663:Semicarbazides / Q000737:chemistry; D000637:Transaminases / Q000737:chemistry*; D014633:Valine / Q000737:chemistry
chemical_list: C502751:2,4-diacetamido-2,4,6-trideoxyglucopyranose; D012663:Semicarbazides; C042448:aldimine eliminator; D000637:Transaminases; D014633:Valine; D008239:Lysine; D004072:Digitonin; D000117:Acetylglucosamine
keywords: N,N'-diacetylbacillosamine; N-glycosylation; aminotransferase; bacterial glycoproteins; pyridoxal 5'-phosphate