title: Silk sericin loaded alginate nanoparticles: Preparation and anti-inflammatory efficacy.
abstract: In this study, silk sericin loaded alginate nanoparticles were prepared by the emulsification method followed by internal crosslinking. The effects of various silk sericin loading concentration on particle size, shape, thermal properties, and release characteristics were investigated. The initial silk sericin loadings of 20, 40, and 80% w/w to polymer were incorporated into these alginate nanoparticles. SEM images showed a spherical shape and small particles of about 71.30-89.50 nm. TGA analysis showed that thermal stability slightly increased with increasing silk sericin loadings. FTIR analysis suggested interactions between alginate and silk sericin in the nanoparticles. The release study was performed in acetate buffer at normal skin conditions (pH 5.5; 32 °C). The release profiles of silk sericin exhibited initial rapid release, consequently with sustained release. These silk sericin loaded alginate nanoparticles were further incorporated into topical hydrogel and their anti-inflammatory properties were studied using carrageenan-induced paw edema assay. The current study confirms the hypothesis that the application of silk sericin loaded alginate nanoparticle gel can inhibit inflammation induced by carrageenan.
authors.affiliation: The Petroleum and Petrochemical College and Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Thitikan
authors.initials: T
authors.lastname: Khampieng
authors.affiliation: Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pornanong
authors.initials: P
authors.lastname: Aramwit
authors.affiliation: The Petroleum and Petrochemical College and Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: pitt.s@chula.ac.th.
authors.forename: Pitt
authors.initials: P
authors.lastname: Supaphol
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D002351:Carrageenan / Q000009:adverse effects; D004195:Disease Models, Animal; D065546:Drug Liberation; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D047030:Sericins / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D000464:Alginates; D000893:Anti-Inflammatory Agents; D006603:Hexuronic Acids; D047030:Sericins; D047011:Silk; D020723:Glucuronic Acid; D002351:Carrageenan
keywords: Alginate nanoparticles; Anti-inflammation; Carrageenan-induced paw edema; Silk sericin