title: Molecular Characterization and Seroprevalence in Pigs of SC0806, a Cat Que Virus Isolated from Mosquitoes in Sichuan Province, China.
abstract: The Simbu serogroup currently consists of a highly diverse group of related arboviruses that infect both humans and economically important livestock species. Cat Que virus (CQV), a Simbu serogroup virus of the genus Orthobunyavirus (family Bunyaviridae), was first isolated in 2004 from mosquitoes during surveillance of arbovirus activity in acute pediatric encephalitis in northern Vietnam. We report here the complete genome sequence of SC0806 isolated from mosquitoes (Culex tritaeniorhynchus) in Sichuan Province, China. Consistent with the genomic organization of Simbu serogroup viruses, the SC0806 genome comprises three RNA segments-a large (L) segment (6928 nucleotides) that encodes the 2261-amino-acid RNA-dependent RNA polymerase, a medium (M) segment (4481 nucleotides) that encodes the 1433-amino-acid polyprotein, and a small (S) segment (984 nucleotides) that encodes a 234-amino-acid nucleocapsid protein and a 95-amino-acid nonstructural protein. The respective lengths of the 5'-untranslated region (UTR) and 3'-UTR of L, M, and S are 56 and 86, 43 and 136, and 44 and 238 nucleotides. Sequence (nucleotide and deduced amino acid) comparison and phylogenetic analysis revealed that SC0806 was closely related to the reported Vietnam isolate CQV. This is the first time that CQV has been isolated in Sichuan Province, China. Anti-SC0806 immunoglobulin M (IgM) and IgG antibodies were found in pigs reared locally, indicating that CQV has formed a natural cycle in the local area. Surveillance of the distribution and pathogenicity of SC0806 should be strengthened.
authors.affiliation: 1 Sichuan Center for Disease Control and Prevention , Chengdu, Sichuan, China .
authors.forename: Jiake
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 2 State Key Laboratory for Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, China .
authors.forename: Jinglin
authors.initials: J
authors.lastname: Wang
authors.affiliation: 2 State Key Laboratory for Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, China .
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: 2 State Key Laboratory for Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, China .
authors.forename: Shihong
authors.initials: S
authors.lastname: Fu
authors.affiliation: 2 State Key Laboratory for Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, China .
authors.forename: Minghua
authors.initials: M
authors.lastname: Li
authors.affiliation: 4 Washington University , St. Louis, Missouri.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Zhao
authors.affiliation: 2 State Key Laboratory for Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, China .
authors.forename: Wuyang
authors.initials: W
authors.lastname: Zhu
authors.affiliation: 4 Washington University , St. Louis, Missouri.
authors.forename: David
authors.initials: D
authors.lastname: Wang
authors.affiliation: 2 State Key Laboratory for Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, China .
authors.forename: Guodong
authors.initials: G
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002681:China / Q000453:epidemiology; D003465:Culex / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D016851:Orthobunyavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D016036:Seroepidemiologic Studies; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology
chemical_list: D012367:RNA, Viral
keywords: Cat Que virus; China; Phylogenetics; Seroprevalence; Whole genome