title: Autoantibodies to chromogranin A are potential diagnostic biomarkers for non-small cell lung cancer.
abstract: Cancer-associated autoantibodies show promise as sensitive biomarkers for the early detection of cancer. To test the immunogenicity of chromogranin A (ChgA) as a B cell autoantigen and to assess the potential applications of ChgA autoantibodies as novel biomarkers for the diagnosis of non-small cell lung cancer (NSCLC), we developed a high-content peptide microarray using ChgA peptides. Autoantibody profiling was carried out using sera from 168 individuals with NSCLC and 97 healthy controls. We present evidence for the occurrence of autoantibodies to ChgA peptides in patient sera and identified five highly responsive peptides in the NSCLC group using significance analysis of microarray (SAM). Receiver operating characteristic analyses showed that ChgA autoantibodies are valuable in the predictive diagnosis of NSCLC, suggesting that serum autoantibodies to ChgA-derived peptides are promising novel markers of NSCLC. Moreover, the high-content peptide microarray antibody profiling reported in this work provides a powerful tool to visualize the overall B cell response to ChgA peptides and should enable the rapid development of in-depth research into ChgA.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, Jiangsu province, 215006, People's Republic of China.
authors.forename: Songnan
authors.initials: S
authors.lastname: Qi
authors.affiliation: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, No. 398, Ruoshui Road, SEID, Suzhou Industrial Park, Suzhou, Jiangsu province, 215123, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Mo
authors.initials: M
authors.lastname: Huang
authors.affiliation: Suzhou SJ Biomaterials Co. Ltd, Suzhou, 215123, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Teng
authors.affiliation: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, No. 398, Ruoshui Road, SEID, Suzhou Industrial Park, Suzhou, Jiangsu province, 215123, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, Jiangsu province, 215006, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, Jiangsu province, 215006, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, Jiangsu province, 215006, People's Republic of China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Shi
authors.affiliation: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, No. 398, Ruoshui Road, SEID, Suzhou Industrial Park, Suzhou, Jiangsu province, 215123, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Gusu District, Suzhou, Jiangsu province, 215006, People's Republic of China. ghgu111@126.com.
authors.forename: Guohao
authors.initials: G
authors.lastname: Gu
authors.affiliation: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, No. 398, Ruoshui Road, SEID, Suzhou Industrial Park, Suzhou, Jiangsu province, 215123, People's Republic of China. yqxin2013@sinano.ac.cn.
authors.forename: Yinqiang
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, No. 398, Ruoshui Road, SEID, Suzhou Industrial Park, Suzhou, Jiangsu province, 215123, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ma
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000276:immunology / Q000473:pathology; D053379:Chromogranin A / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D014408:Biomarkers, Tumor; D053379:Chromogranin A
keywords: Antigenic sites; ChgA autoantibodies; NSCLC; Peptide microarray