title: In silico prediction of proteins related to xyloglucan fucosyltransferases in Solanaceae genomes.
abstract: Two independent studies have shown that the cell wall of pollen tubes from tobacco and tomato species contained fucosylated xyloglucan (XyG). These findings are intriguing as many reports have shown that XyG of somatic cells of these species is not fucosylated but instead is arabinosylated. In order to produce fucosylated XyG, plants must express a functional galactoside α-2-fucosyltransferase. Here, using a bioinformatics approach, we show that several candidate genes coding for XyG fucosyltransferases are present in the genome of coffee and several Solanaceae species including tomato, tobacco, potato, eggplant and pepper. BLAST and protein alignments with the 2 well-characterized XyG fucosyltransferases from Arabidopsis thaliana and Pisum sativum revealed that at least 6 proteins from different Solanaceae species and from coffee displayed the 3 conserved motifs required for XyG fucosyltransferase activity.
authors.affiliation: a Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale EA4358 ; IRIB; VASI; Normandy University; University of Rouen ; Mont-Saint-Aignan cedex , France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lehner
authors.forename: Laurence
authors.initials: L
authors.lastname: Menu-Bouaouiche
authors.forename: Flavien
authors.initials: F
authors.lastname: Dardelle
authors.forename: François
authors.initials: F
authors.lastname: Le Mauff
authors.forename: Azeddine
authors.initials: A
authors.lastname: Driouich
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Mollet
mesh_terms: D000465:Algorithms; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D040503:Coffea / Q000201:enzymology; D003198:Computer Simulation; D005647:Fucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D019657:Solanaceae / Q000201:enzymology* / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D005647:Fucosyltransferases; C070983:xyloglucan 2-fucosyltransferase
keywords: AA, amino acid; AGP, arabinogalactan proteins; Capsicum annuum; Coffea canephora; Eggplant; FUT, fucosyltransferase; Nicotiana benthamiana; Nicotiana tabacum; Solanum lycopersicum; Solanum melongena; Solanum tuberosum; XyG, xyloglucan.; cell wall; coffee; pepper; pollen; potato; tobacco; tomato; xyloglucan; xyloglucan fucosyltransferase.