title: Nogo-B protects mice against lipopolysaccharide-induced acute lung injury.
abstract: Nogo-B, a member of the reticulon 4 protein family, plays a critical role in tissue repair and acute inflammation. Its role in acute lung injury (ALI) remains unclear. Here, we assessed the function of Nogo-B during tissue injury in a lipopolysaccharide (LPS)-induced ALI mouse model. We found that pulmonary Nogo-B was significantly repressed after LPS instillation in C57BL/6 mice. Over-expression of pulmonary Nogo-B using an adenovirus vector carrying the Nogo-B-RFP-3flag gene (Ad-Nogo-B) significantly prolonged the survival of mice challenged with a lethal dose of LPS. The Ad-Nogo-B-treated mice also had less severe lung injury, less alveolar protein exudation, and a higher number of macrophages but less neutrophil infiltration compared with Ad-RFP-treated mice. Interestingly, microarray analysis showed that the Ad-Nogo-B-treated mice had different gene expression profiles compared with the controls and the prominent expression of genes related to wound healing and the humoral immune response after LPS induction. Of the 49 differently expressed genes, we found that the expression of PTX3 was significantly up-regulated following Nogo-B over-expression as observed in lung tissues and RAW264.7 cells. In conclusion, Nogo-B plays a protective role against LPS-induced ALI, and this effect might be exerted through the modulation of alveolar macrophage recruitment and PTX3 production.
authors.affiliation: 1] Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China [2] Department of Respiratory Medicine, Jinling Hospital, Nanjing, China.
authors.forename: Wujian
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China.
authors.forename: Yunye
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Disease, Changhai Hospital, Second Military Medical University, China.
authors.forename: Qinying
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000401:mortality / Q000473:pathology*; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004195:Disease Models, Animal; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D056724:Immunity, Humoral; D008070:Lipopolysaccharides / Q000276:immunology / Q000633:toxicity*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009185:Myelin Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000070798:Nogo Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000683:Serum Amyloid P-Component / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D059467:Transcriptome; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D008070:Lipopolysaccharides; D009185:Myelin Proteins; D000070798:Nogo Proteins; D034741:RNA, Small Interfering; D000683:Serum Amyloid P-Component; D000099153:Pentraxins; C000603201:Rtn4 protein, mouse