title: Characterization of the nuclear import and export signals of pseudorabies virus UL31.
abstract: The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pUL31, which is a multifunctional protein that is important for HSV-1 infection. However, little is known concerning the subcellular localization signal of PRV UL31. Here, by transfection with a series of PRV UL31 deletion mutants fused to an enhanced yellow fluorescent protein (EYFP) gene, a bipartite nuclear localization signal (NLS) and a PY motif NLS of UL31 were identified and mapped to amino acids (aa) 4 to 20 (RRRLLRRKSSAARRKTL) and aa 21 to 34 (TRAARDRYAPYFAY), respectively. Additionally, the predicted nuclear export signal (NES) was shown to be nonfunctional. Taken together, this information opens up new avenues for investigating the biological functions of UL31 during PRV infection.
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.|Guangzhou Hoffmann Institute of Immunology, School of Basic Science, Guangzhou Medical University, Guangzhou, 511436, People's Republic of China.
authors.forename: Meili
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Si
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Chuncong
authors.initials: C
authors.lastname: Mo
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Zhancheng
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Yanjia
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Chunke
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Guangdong Haid Group Co., Ltd., Guangzhou, 511400, People's Republic of China.
authors.forename: Jinlu
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangzhou Hoffmann Institute of Immunology, School of Basic Science, Guangzhou Medical University, Guangzhou, 511436, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou, 511436, Guangdong, People's Republic of China. mingshengcai@hotmail.com.|Guangzhou Hoffmann Institute of Immunology, School of Basic Science, Guangzhou Medical University, Guangzhou, 511436, People's Republic of China. mingshengcai@hotmail.com.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Cai
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D021381:Protein Transport; D011557:Pseudorabies / Q000378:metabolism / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D019913:Nuclear Localization Signals; C000592811:UL31 protein, pseudorabies virus; D014764:Viral Proteins
keywords: Nuclear export signal (NES); Nuclear localization signal (NLS); PRV UL31