title: Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining.
abstract: Apoptosis in hair follicles often is studied under pathological conditions; little is known about apoptotic mechanisms during normal hair follicle formation and maintenance. We investigated proteins of intrinsic apoptotic pathway, Bim and Puma, during hair follicle development and the first catagen stage using immunofluorescence to describe their expression patterns and to correlate them with apoptosis as determined by TUNEL assay. Both proteins were found in developing follicles. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. Our findings suggest that these primary apoptotic molecules participate in postnatal development and maintenance of hair follicles.
authors.affiliation: a Institute of Animal Physiology and Genetics AS CR , Brno , Czech Republic.|b Department of Experimental Biology , Masaryk University , Brno , Czech Republic.
authors.forename: B
authors.initials: B
authors.lastname: Vesela
authors.affiliation: a Institute of Animal Physiology and Genetics AS CR , Brno , Czech Republic.|c Department of Physiology , University of Veterinary and Pharmaceutical Sciences , Brno , Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Matalova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D000072224:Bcl-2-Like Protein 11; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D020287:In Situ Nick-End Labeling / Q000379:methods*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D000072224:Bcl-2-Like Protein 11; C000606776:Bcl2l11 protein, mouse; D008565:Membrane Proteins; C488588:PUMA protein, mouse; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins
keywords: Bim; Puma; TUNEL; apoptosis; development; hair follicle; immunofluorescence