title: The Role of Circulating Platelets Microparticles and Platelet Parameters in Acute Ischemic Stroke Patients.
abstract: BACKGROUND
  Platelet activation and aggregation are critical in the pathogenesis of acute ischemic stroke (AIS). Circulating platelet microparticles (PMPs) and platelet parameters are biologic markers of platelet function in AIS patients; however, their associations with stroke subtypes and infarct volume remain unknown.
  
  
  METHODS
  We recruited 112 AIS patients including large-artery atherosclerosis (LAA) and small-artery occlusion [SAO] subtypes and 35 controls in this study. Blood samples were collected at admission and after antiplatelet therapy. The levels of circulating PMPs and platelet parameters (mean platelet volume [MPV], platelet count, plateletocrit, and platelet distribution width) were determined by flow cytometry and hematology analysis, respectively. Infarct volume was examined at admission by magnetic resonance imaging.
  
  
  RESULTS
  (1) The levels of circulating PMPs and MPV were significantly elevated in AIS patients compared with healthy controls; (2) the level of circulating PMPs, but not platelet parameters, was decreased after antiplatelet therapy in AIS patients; (3) the infarct volume in LAA subtype was larger than that in SAO subtype. Notably, circulating PMP level was positively correlated with the infarct volume in LAA subtype. No association with infarct volume in either AIS subtype was observed for platelet parameters; and (4) according to the regression analysis, circulating PMP was an independent risk factor for the infarct volume in pooled AIS patients after adjustments of other impact factors (hypertension and diabetes).
  
  
  CONCLUSIONS
  Our results suggest that circulating PMP level is associated with cerebral injury of AIS, which offers a novel evaluation parameter for AIS patients.
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Yusen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Caixia
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China; Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio.
authors.forename: Ji C
authors.initials: JC
authors.lastname: Bihl
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China. Electronic address: mxtgdmc@163.com.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China; Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio. Electronic address: yanfang.chen@wright.edu.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000368:Aged; D001792:Blood Platelets / Q000473:pathology; D020520:Brain Infarction / Q000097:blood* / Q000209:etiology / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016015:Logistic Models; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use; D010976:Platelet Count; D012189:Retrospective Studies; D012307:Risk Factors; D020521:Stroke / Q000097:blood* / Q000188:drug therapy / Q000209:etiology
chemical_list: D010975:Platelet Aggregation Inhibitors
keywords: Acute ischemic stroke; circulating platelet microparticles; infarct volume; platelet parameters