title: Molecular characterization and expression analysis of interferon-gamma in the large yellow croaker Larimichthys crocea.
abstract: The large yellow croaker Larimichthys crocea is an important mariculture fish species in China, and the bacterium Vibrio harveyi (V. harveyi) and the ciliate protozoan Cryptocaryon irritans (C. irritans) are the two major pathogens in its aquaculture sector. Interferon-gamma (IFN-γ) plays important roles in regulating both innate and cell mediated immune responses as an inflammatory cytokine. In this study, we obtained the nucleotide sequence of IFN-γ from the large yellow croaker (LcIFN-γ). The phylogenetic relationship tree of 18 available IFN-γ genes was constructed based on their sequences. Expression analyses in 10 various tissues were conducted after the croaker challenged with V. harveyi and C. irritans, respectively. Real time PCR analysis showed that the expression of LcIFN-γ was observed broadly in health individuals. After injected with V. harveyi, the 10 tissues had a higher expression of IFN-γ at the first day (1 d); only spleen, muscle, intestine, heart and skin had higher expressions after infected with C. irritans at 1 d. Major immune tissues (skin, gill, head kidney and spleen) and detoxification tissue (liver) were sampled at 0 h, 6 h, 1 d, 2 d, 3 d, 4 d, 5 d and 7 d to understand the expression trends of LcIFN-γ after challenged with C. irritans. The expressions of LcIFN-γ in skin and gill (the primary immune organs) showed a clear correlative relationship with the life cycle of C. irritans.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Ruan-Ni
authors.initials: RN
authors.lastname: Chen
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Su
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China. Electronic address: maoyong@xmu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Ningde Fufa Fisheries Co., LTD, Ningde, 352002, China.
authors.forename: Qiao-Zhen
authors.initials: QZ
authors.lastname: Ke
authors.affiliation: Ningde Fufa Fisheries Co., LTD, Ningde, 352002, China.
authors.forename: Kun-Huang
authors.initials: KH
authors.lastname: Han
authors.affiliation: Ningde Fufa Fisheries Co., LTD, Ningde, 352002, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Zheng
authors.affiliation: Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Jian-She
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016798:Ciliophora / Q000502:physiology; D016770:Ciliophora Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes; D016415:Sequence Alignment / Q000662:veterinary; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D007371:Interferon-gamma
keywords: Cryptocaryon irritans; Gene expression; Interferon-gamma; Larimichthys crocea; Vibrio harveyi