title: Interleukin-6 (IL-6) mediated the increased contraction of distal colon in streptozotocin-induced diabetes in rats via IL-6 receptor pathway.
abstract: Colonic dysmotility occurs in diabetes and blood plasma interleukin (IL)-6 levels are significantly elevated in type 1 diabetes mellitus. The aim of this study was to investigate whether IL-6 and the IL-6 receptor pathway mediates colonic dysfunction in type 1 diabetes mellitus. Male SD rats were treated with a single intraperitoneally injected dose of streptozotocin (STZ), and those displaying sustained high blood glucose were selected as diabetes mellitus models. Longitudinal muscle strips of colon were prepared to monitor colonic contraction in vitro. Contractile responses of strips of colon were recorded following treatment with IL-6 in control animals, and following anti IL-6 antibody treatment in STZ-induced diabetes in rats. Concentration of IL-6 in plasma and colon were determined by ELISA. Expressions of IL-6 α-receptor and IL-6 β-receptor in colon tissues were determined by immunohistochemistry or Western blot analysis. The non-diabetes rats treated with IL-6 and the untreated diabetes rats showed increased contraction of distal colon, whereas the diabetes rats treated with anti-IL-6 antibody showed decreased contraction of distal colon compared with the untreated diabetes rats. The IL-6 levels of plasma but not colon increased in diabetes rats. The expression of IL-6 α-receptor increased in diabetes rats. These results indicate that diabetes rats show an increase in the contractions of distal colon partly via the IL-6-IL-6 receptor pathway.
authors.affiliation: Department of Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Xin-Wen
authors.initials: XW
authors.lastname: Chang
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Tao-Fang
authors.initials: TF
authors.lastname: Xi
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Ze-Hao
authors.initials: ZH
authors.lastname: Lu
authors.affiliation: Department of Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Cai
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200092, China.
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Xie
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D050822:Cytokine Receptor gp130 / Q000819:agonists* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000503:physiopathology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000503:physiopathology*; D004305:Dose-Response Relationship, Drug; D005769:Gastrointestinal Motility / Q000187:drug effects*; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000097:blood / Q000494:pharmacology*; D053698:Interleukin-6 Receptor alpha Subunit / Q000819:agonists* / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: C494201:Il6st protein, rat; D015850:Interleukin-6; D053698:Interleukin-6 Receptor alpha Subunit; D050822:Cytokine Receptor gp130; D002217:Carbachol
keywords: Diabetes mellitus; IL-6; IL-6 receptor; colon motility