title: Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.
abstract: Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In this study, native interactions of C. albicans with macrophages were investigated by proteome analysis using high efficiency of long monolithic silica capillary column. Using this system, we developed a method of "mixed and quantitative proteome analysis" in which C. albicans and macrophages were simultaneously analyzed by nanoLC-MS/MS without the need to isolate the two individual living cells. Two hundred twenty-seven proteins from C. albicans and five proteins from macrophages were identified as candidate interaction-specific molecules. C. albicans seemed to produce glucose through a β-oxidation pathway, a glyoxylate cycle, and gluconeogenesis for escape from macrophages. Up-regulation of stress-related and candidate pathogenic proteins in C. albicans indicated how C. albicans endured the harsh environment inside the macrophages. Down-regulation of apoptosis-associated protein NOA1- and chaperone HSPA1A-syntheses in macrophage indicated that C. albicans was able to escape from macrophages in part by suppressing the production of these macrophage proteins.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan, kitanao@kais.kyoto-u.ac.jp.
authors.forename: Nao
authors.initials: N
authors.lastname: Kitahara
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Takeda
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibasaki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda