title: Stability of coagulation protein activities in single units or pools of cryoprecipitate during storage at 20-24°C for up to 24 h.
abstract: OBJECTIVE
  Cryoprecipitate is a concentrated source of fibrinogen and other plasma proteins. Cryoprecipitate must be transfused within 4-6 h of thawing and storage at 20-24°C. We compared plasma protein activities in single or pooled cryoprecipitate units stored at 20-24°C for 0, 4 or 24 h.
  
  
  METHODS
  Individual cryoprecipitate units (n = 36) were thawed, diluted with sterile saline and sampled over time. Cryoprecipitate pools of eight individual units were assembled either by serial passage of diluent (Method A, n = 6 pools) or by separate dilution into a single collection bag (Method B, n = 6 pools). Fibrinogen, factor VIII, factor XIII and von Willebrand factor activities were measured.
  
  
  RESULTS
  No significant losses in activities were found relative to at-thaw values after either 4 or 24 h of storage of individual cryoprecipitate units at 20-24°C; 35 of 36 units contained >150 mg of fibrinogen. No significant differences were found between activities in single vs. pooled units of cryoprecipitate assembled using either method, or between cryoprecipitate pools made by Method A (80-160 ml volume) or Method B (160-240 ml volume) at 0, 4 or 24 h post-thaw; freezing and thawing of pools did not lead to significant activity losses.
  
  
  CONCLUSIONS
  The stability of fibrinogen and other factors in thawed cryoprecipitate stored at 20-24°C suggests that the shelf life may be safely extended to 24 h provided that sterility is maintained.
authors.affiliation: Canadian Blood Services Centre for Innovation, McMaster University, Hamilton, ON, Canada.|Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: W P
authors.initials: WP
authors.lastname: Sheffield
authors.affiliation: Canadian Blood Services Centre for Innovation, McMaster University, Hamilton, ON, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Bhakta
authors.affiliation: Canadian Blood Services Centre for Innovation, Ottawa, ON, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Jenkins
mesh_terms: D001793:Blood Preservation / Q000009:adverse effects* / Q000379:methods; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D005615:Freezing / Q000009:adverse effects*; D006801:Humans; D055550:Protein Stability
chemical_list: D005340:Fibrinogen
keywords: blood component production; blood components; blood processing; plasma derivatives