title: A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities.
abstract: A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.
authors.affiliation: Institute for Systems Biology, Seattle, WA, USA. Electronic address: athompso@systemsbiology.org.
authors.forename: Anne W
authors.initials: AW
authors.lastname: Thompson
authors.affiliation: BD Biosciences, Advanced Cytometry Group, Seattle, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Crow
authors.affiliation: BD Biosciences, Advanced Cytometry Group, Seattle, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Wadey
authors.affiliation: Institute for Systems Biology, Seattle, WA, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Arens
authors.affiliation: Institute for Systems Biology, Seattle, WA, USA.
authors.forename: Serdar
authors.initials: S
authors.lastname: Turkarslan
authors.affiliation: Institute for Systems Biology, Seattle, WA, USA.
authors.forename: Sergey
authors.initials: S
authors.lastname: Stolyar
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Elliott
authors.affiliation: BD Biosciences, Advanced Cytometry Group, Seattle, USA.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Petersen
authors.affiliation: Center for Marine Cytometry, Birdsview, WA, USA.
authors.forename: Ger
authors.initials: G
authors.lastname: van den Engh
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Stahl
authors.affiliation: Institute for Systems Biology, Seattle, WA, USA; Departments of Biology and Microbiology, University of Washington, Seattle, WA, USA; Molecular and Cellular Biology Program, University of Washington, Seattle, WA, USA; Lawrence Berkeley National Lab, Berkeley, CA, USA.
authors.forename: Nitin S
authors.initials: NS
authors.lastname: Baliga
mesh_terms: D001421:Bacteria, Anaerobic / Q000166:cytology / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D050296:Microbial Viability; D059010:Single-Cell Analysis
keywords: Anaerobic microorganism; Controlled-environment sorting; Flow cytometry; Single cells