title: Comprehensive identification of 125 multifarious constituents in Shuang-huang-lian powder injection by HPLC-DAD-ESI-IT-TOF-MS.
abstract: A high-performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method was established for excellent separation and structural identification of constituents in Shuang-huang-lian powder injection (SHLPI). The typical ultraviolet absorptions, accurate empirical molecular formula and reasonable fragmentation mechanisms of these ingredients were used for their structural elucidation. In consequence, 125 constituents (33 phenolic acids, 29 flavonoids, 32 phenylethanoid glycosides, 15 iridoid glycosides, 8 lignans, 3 amino acids and 2 purines nucleosides, 2 quinoid glycosides and 1 alkylbenzene glycoside) were either unequivocally identified or tentatively characterized by comparing authentic standards or published data. The result showed that this study could provide valuable information for the quality control and further investigation of SHLPI formula.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: shy19880610@163.com.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Meixian
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors.forename: Zongtao
authors.initials: Z
authors.lastname: Lin
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Haixiu
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Niu
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: chenbjmu@163.com.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Chen
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D007267:Injections; D017705:Lignans / Q000032:analysis*; D011208:Powders; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000596:Amino Acids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D062385:Hydroxybenzoates; D017705:Lignans; D011208:Powders; C516400:shuang-huang-lian; C017616:phenolic acid
keywords: Flavonoids; HPLC-DAD-ESI-IT-TOF-MS; Iridoid glycosides; Phenolic acids; Phenylethanoid glycosides; Shuang–huang–lian powder injection