title: Isolation and characterization of collagen from the skin of Brama australis.
abstract: Collagen was extracted from the skin of Brama australis, the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SDS-PAGE, and amino acid analysis. The denaturation temperature (T(d)) of obtained collagen was found to be 24°C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different α-chains (α1 and α2) and was classified as type I collagen. Although T(d) of obtained collagen is higher than 20 °C it is still far from T(d) of mammalian collagen.
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, ul. Gagarina 7, 87-100 Toruń, Poland. Electronic address: as@chem.umk.pl.
authors.forename: Alina
authors.initials: A
authors.lastname: Sionkowska
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, ul. Gagarina 7, 87-100 Toruń, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Kozłowska
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, ul. Gagarina 7, 87-100 Toruń, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Skorupska
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, ul. Gagarina 7, 87-100 Toruń, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Michalska
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification*; D024042:Collagen Type I / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D011489:Protein Denaturation; D012867:Skin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000596:Amino Acids; D024042:Collagen Type I; D003094:Collagen
keywords: Collagen; Denaturation temperature; Fish skin