title: Genetic and morphological characterization of Trichuris myocastoris found in Myocastor coypus in the Czech Republic.
abstract: Trichuris sp. individuals were collected from Myocastor coypus from fancy breeder farms in the Czech Republic. Using morphological and biometrical methods, 30 female and 30 male nematodes were identified as Trichuris myocastoris. This paper presents the first molecular description of this species. The ribosomal DNA (rDNA) region, consisting of internal transcribed spacer (ITS)-1, 5.8 gene and ITS-2, was sequenced. Based on an analysis of 651 bp, T. myocastoris was found to be different from any other Trichuris species for which published sequencing of the ITS region is available. The phylogenetic relationships were estimated using the maximum parsimony methods and Bayesian analyses. T. myocastoris was found to be significantly closely related to Trichuris of rodents than those of ruminants.
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Rylková
authors.affiliation: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Tůmová
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Brožová
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Jankovská
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Vadlejch
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Čadková
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Frýdlová
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Peřinková
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic. langrova@af.czu.cz.
authors.forename: I
authors.initials: I
authors.lastname: Langrová
authors.affiliation: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Chodová
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: S
authors.initials: S
authors.lastname: Nechybová
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague, 6-Suchdol, Czech Republic.
authors.forename: Š
authors.initials: Š
authors.lastname: Scháňková
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D018153:Czech Republic / Q000453:epidemiology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D010802:Phylogeny; D014257:Trichuriasis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014258:Trichuris / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer
keywords: ITS1-5.8S-ITS2; Myocastor coypus; Parasites; Trichuris myocastoris; rDNA