title: Identification of ribonucleotide reductase mutation causing temperature-sensitivity of herpes simplex virus isolates from whitlow by deep sequencing.
abstract: Herpes simplex virus 2 caused a genital ulcer, and a secondary herpetic whitlow appeared during acyclovir therapy. The secondary and recurrent whitlow isolates were acyclovir-resistant and temperature-sensitive in contrast to a genital isolate. We identified the ribonucleotide reductase mutation responsible for temperature-sensitivity by deep-sequencing analysis.
authors.affiliation: Department of Virology, University of Toyama Toyama, 930-0194, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Daikoku
authors.affiliation: Department of Virology, University of Toyama Toyama, 930-0194, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Oyama
authors.affiliation: Department of Virology, University of Toyama Toyama, 930-0194, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Yajima
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sekizuka
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: Department of Dermatology, Kanazawa University Graduate School of Medical Science Kanazawa, Ishikawa, 920-8640, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Shimada
authors.affiliation: Department of Dermatology, Kanazawa University Graduate School of Medical Science Kanazawa, Ishikawa, 920-8640, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takehara
authors.affiliation: Department of Gynecology and Obstetrics, University of Toyama Toyama, 930-0194, Japan ; Department of Obstetrics and Gynecology, Shakaihoken Takaoka Hospital Takaoka, Toyama, 933-0115, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Miwa
authors.affiliation: Department of Virology, University of Toyama Toyama, 930-0194, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Okuda
authors.affiliation: Department of Virology, Toyama Institute of Health Toyama, 939-0363, Japan.
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.affiliation: Department of Virology, University of Toyama Toyama, 930-0194, Japan.
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Shiraki
keywords: Herpes simplex virus; high-throughput DNA sequencing; reactivation; ribonucleotide reductase; temperature-sensitive (Ts); thymidine kinase deficient