title: MiR-26b inhibits hepatocellular carcinoma cell proliferation, migration, and invasion by targeting EphA2.
abstract: Deregulated microRNAs (miRNAs) have been shown to play important roles in cancer progression as a result of changes in expression of their target genes. In this study, we investigated the expression of miR-16b in eight hepatocellular carcinoma (HCC) cell lines, revealed the roles of miR-26b on hepatocellular carcinoma (HCC) cell proliferation, migration, and invasion, and confirmed that EphA2 is a direct target of miR-26b. The miR-26b expression was decreased and EphA2 expression was evaluated in HCC cell lines. Luciferase assays revealed that miR-26b inhibited EphA2 expression by targeting the 3'-untranslated region of EphA2 mRNA. Overexpression of miR-26b dramatically inhibited the proliferation, invasion, and migration of HCC cells by targeting EphA2. Moreover, miR-26b down-regulated c-Myc and CyclinD1 expression, which was reversed by overexpressed EphA2. Taken together, our data demonstrated the mechanism of miR-26b contributed to HCC progression and implicated that miR-26b's potential in HCC therapy.
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong Province, China ; Department of Gastrointestinal Surgery, The Affiliated Hospital of Binzhou Medical University Binzhou 256603, China.
authors.forename: Hesheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong Province, China ; Department of Gastrointestinal Surgery, The Affiliated Hospital of Binzhou Medical University Binzhou 256603, China.
authors.forename: Qinglei
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Medicine and Nursing, Dezhou University Dezhou 253023, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Gastrointestinal Surgery, The Affiliated Hospital of Binzhou Medical University Binzhou 256603, China.
authors.forename: Xingquan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Gastrointestinal Surgery, The Affiliated Hospital of Binzhou Medical University Binzhou 256603, China.
authors.forename: Baoguang
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong Province, China.
authors.forename: Sanyuan
authors.initials: S
authors.lastname: Hu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D036104:Receptor, EphA2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D036104:Receptor, EphA2; C528163:MIRN26 microRNA, human
keywords: EphA2; hepatocellular carcinoma; invasion; miR-26b; migration; proliferation