title: Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells.
abstract: This report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells in vivo and in vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells into mouse salivary glands. However, the tumorigenicity of iPS cells has not been resolved yet. It is well known that stem cells affect their microenvironment, known as a stem cell niche. We focused on the niche and the interaction between iPS cells and salivary gland cells in our study on salivary gland regeneration. Coculture of embryonic SMG cells and iPS cells have better-developed epithelial structures and fewer undifferentiated specific markers than monoculture of embryonic SMG cells in vitro. These results suggest that iPS cells have a potential ability to accelerate differentiation for salivary gland development and regeneration.
authors.affiliation: Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ono
authors.affiliation: Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Obana
authors.affiliation: Division of Orthopedic Surgery, The Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Usami
authors.affiliation: Clinical Laboratory, Osaka University Dental Hospital, Suita, Osaka 565-0871, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Sakai
authors.affiliation: Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Nohara
authors.affiliation: Division of Molecular and Regenerative Prosthodontics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egusa
authors.affiliation: Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation*; D051379:Mice; D012038:Regeneration / Q000502:physiology*; D012469:Salivary Glands / Q000166:cytology* / Q000254:growth & development*; D055153:Stem Cell Niche / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods