title: Tangential migration of neuronal precursors of glutamatergic neurons in the adult mammalian brain.
abstract: In a classic model of mammalian brain formation, precursors of principal glutamatergic neurons migrate radially along radial glia fibers whereas GABAergic interneuron precursors migrate tangentially. These migration modes have significant implications for brain function. Here we used clonal lineage tracing of active radial glia-like neural stem cells in the adult mouse dentate gyrus and made the surprising discovery that proliferating neuronal precursors of glutamatergic granule neurons exhibit significant tangential migration along blood vessels, followed by limited radial migration. Genetic birthdating and morphological and molecular analyses pinpointed the neuroblast stage as the main developmental window when tangential migration occurs. We also developed a partial "whole-mount" dentate gyrus preparation and observed a dense plexus of capillaries, with which only neuroblasts, among the entire population of progenitors, are directly associated. Together, these results provide insight into neuronal migration in the adult mammalian nervous system.
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Sun
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Biochemistry, Cellular and Molecular Biology Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Predoctoral Human Genetics Training Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Ryan P
authors.initials: RP
authors.lastname: Stadel
authors.affiliation: Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland;
authors.forename: Jonathan
authors.initials: J
authors.lastname: Moss
authors.affiliation: Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Jing Hui A
authors.initials: JH
authors.lastname: Yong
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Shiori
authors.initials: S
authors.lastname: Ito
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Kawasaki
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Alexander T
authors.initials: AT
authors.lastname: Phan
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Justin H
authors.initials: JH
authors.lastname: Oh
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Nikhil
authors.initials: N
authors.lastname: Modak
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
authors.forename: Randall R
authors.initials: RR
authors.lastname: Reed
authors.affiliation: Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland;
authors.forename: Nicolas
authors.initials: N
authors.lastname: Toni
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Biochemistry, Cellular and Molecular Biology Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Predoctoral Human Genetics Training Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205; shongju1@jhmi.edu gming1@jhmi.edu.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Song
authors.affiliation: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Biochemistry, Cellular and Molecular Biology Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205 shongju1@jhmi.edu gming1@jhmi.edu.
authors.forename: Guo-li
authors.initials: GL
authors.lastname: Ming
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods; D002465:Cell Movement; D018891:Dentate Gyrus / Q000502:physiology*; D005260:Female; D005973:Glutamine / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000502:physiology; D021621:Imaging, Three-Dimensional; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008959:Models, Neurological; D058953:Neural Stem Cells / Q000166:cytology; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*
chemical_list: D005973:Glutamine; D049452:Green Fluorescent Proteins
keywords: adult neurogenesis; hippocampus; lineage tracing; migration; vascular niche