title: Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation.
abstract: The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.
authors.affiliation: IOM Ricerca, 95029 Viagrande, Italy. giovanna.calabrese@grupposamed.com.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Calabrese
authors.affiliation: IOM Ricerca, 95029 Viagrande, Italy. rogulino@unict.it.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Giuffrida
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95125 Catania, Italy. lofurno@unict.it.
authors.forename: Debora
authors.initials: D
authors.lastname: Lo Furno
authors.affiliation: Department of Experimental Oncology, Mediterranean Institute of Oncology, 95029 Viagrande, Italy. lauraparrinello@tiscali.it.|Department of Biomedical Sciences, Hematology Section, University of Catania, 95124 Catania, Italy. lauraparrinello@tiscali.it.
authors.forename: Nunziatina Laura
authors.initials: NL
authors.lastname: Parrinello
authors.affiliation: IOM Ricerca, 95029 Viagrande, Italy. stefano.forte@grupposamed.com.
authors.forename: Stefano
authors.initials: S
authors.lastname: Forte
authors.affiliation: IOM Ricerca, 95029 Viagrande, Italy. giuffros@unict.it.|Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95125 Catania, Italy. giuffros@unict.it.
authors.forename: Rosario
authors.initials: R
authors.lastname: Gulino
authors.affiliation: Department of Experimental Oncology, Mediterranean Institute of Oncology, 95029 Viagrande, Italy. cristina.colarossi@grupposamed.com.
authors.forename: Cristina
authors.initials: C
authors.lastname: Colarossi
authors.affiliation: Department of Experimental Oncology, Mediterranean Institute of Oncology, 95029 Viagrande, Italy. luciana.schinocca@grupposamed.com.
authors.forename: Luciana Rita
authors.initials: LR
authors.lastname: Schinocca
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95125 Catania, Italy. giuffros@unict.it.
authors.forename: Rosario
authors.initials: R
authors.lastname: Giuffrida
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95125 Catania, Italy. cardile@unict.it.
authors.forename: Venera
authors.initials: V
authors.lastname: Cardile
authors.affiliation: IOM Ricerca, 95029 Viagrande, Italy. lorenzo.memeo@grupposamed.com.|Department of Experimental Oncology, Mediterranean Institute of Oncology, 95029 Viagrande, Italy. lorenzo.memeo@grupposamed.com.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Memeo
mesh_terms: D050156:Adipogenesis; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010012:Osteogenesis; D010641:Phenotype; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*
chemical_list: C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor
keywords: CD271; adipogenic differentiation; adipose tissue; bone marrow; chondrogenic differentiation; mesenchymal stromal cells; osteogenic differentiation