title: Registered report: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis.
abstract: The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replicating selected results from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012 were selected on the basis of citations and Altimetric scores (Errington et al., 2014). This Registered report describes the proposed replication plan of key experiments from 'Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis' by Goetz and colleagues, published in Cell in 2011 (Goetz et al., 2011). The key experiments being replicated are those reported in Figures 7C (a-d), Supplemental Figure S2A, and Supplemental Figure S7C (a-c) (Goetz et al., 2011). In these experiments, which are a subset of all the experiments reported in the original publication, Goetz and colleagues show in a subcutaneous xenograft model that stromal caveolin-1 remodels the intratumoral microenvironment, which is correlated with increased metastasis formation. The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published in eLife.
authors.affiliation: Transgenics and Genetic Constructs Shared Resource Center, Dartmouth University, Lebanon, United States.
authors.forename: Steven
authors.initials: S
authors.lastname: Fiering
authors.affiliation: Confocal Imaging Core, Harvard Medical School, Boston, United States.
authors.forename: Lay-Hong
authors.initials: LH
authors.lastname: Ang
authors.affiliation: MIA Cellavie Inc., Montreal, Canada.
authors.forename: Judith
authors.initials: J
authors.lastname: Lacoste
authors.affiliation: University of California, Irvine, Irvine, United States.
authors.forename: Tim D
authors.initials: TD
authors.lastname: Smith
authors.affiliation: University of Virginia, Charlottesville, United States.
authors.forename: Erin
authors.initials: E
authors.lastname: Griner
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism*; D004195:Disease Models, Animal; D009362:Neoplasm Metastasis / Q000473:pathology*; D009369:Neoplasms / Q000473:pathology*
chemical_list: D051242:Caveolin 1
keywords: Reproducibility Project: Cancer Biology; biochemical remodeling; biophysics; cell biology; human; metastasis; methodology; mouse; structural biology; tumor microenvironment