title: Elucidation of the growth delimitation of Dunaliella tertiolecta under nitrogen stress by integrating transcriptome and peptidome analysis.
abstract: Dunaliella tertiolecta LB 999 is an oleaginous microalgae species that produces large quantities of lipid and starch during nitrogen starvation; however, nitrogen starvation also limits the cell growth. In order to understand the underlying mechanisms of this phenomenon, the transcriptome and peptidome of D. tertiolecta LB 999 grown under different nitrogen and light conditions were analyzed. Integration of the de novo assembly of transcriptome sequencing reads with peptidome analysis revealed 13,861 protein-coding transcripts, including 33 transcripts whose expression patterns were significantly altered along with the growth phenotypes. Interestingly, 21 of these genes, which were highly enriched in the plastid region, were associated with chlorophyll synthesis and tetrahydrofolate-mediated C1 metabolism. Furthermore, intracellular glutamate levels are predicted to be the main factor that acts as a switch for the regulation of cell growth and carbon accumulation. These data provide the genetic information of D. tertiolecta for its future applications.
authors.affiliation: Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: HyeonSeok
authors.initials: H
authors.lastname: Shin
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea.
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Gachon University, Incheon 406-840, Republic of Korea.
authors.forename: Hyojin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Yoo
authors.affiliation: College of Pharmacy, Gachon University, Incheon 406-840, Republic of Korea.
authors.forename: Hookeun
authors.initials: H
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Hyung-Kyoon
authors.initials: HK
authors.lastname: Choi
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea.
authors.forename: Choul-Gyun
authors.initials: CG
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea. Electronic address: bcho@kaist.ac.kr.
authors.forename: Byung-Kwan
authors.initials: BK
authors.lastname: Cho
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000378:metabolism; D000460:Chlorophyta / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008027:Light; D058086:Microalgae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D002241:Carbohydrates; D005227:Fatty Acids; D020543:Proteome; D002244:Carbon; D009584:Nitrogen
keywords: De novo transcriptome assembly; Dunaliella tertiolecta; Growth limitation; Microalgae; Peptidome analysis