title: Proteins involved in wine aroma compounds metabolism by a Saccharomyces cerevisiae flor-velum yeast strain grown in two conditions.
abstract: A proteomic and exometabolomic study was conducted on Saccharomyces cerevisiae flor yeast strain growing under biofilm formation condition (BFC) with ethanol and glycerol as carbon sources and results were compared with those obtained under no biofilm formation condition (NBFC) containing glucose as carbon source. By using modern techniques, OFFGEL fractionator and LTQ-Orbitrap for proteome and SBSE-TD-GC-MS for metabolite analysis, we quantified 84 proteins including 33 directly involved in the metabolism of glycerol, ethanol and 17 aroma compounds. Contents in acetaldehyde, acetic acid, decanoic acid, 1,1-diethoxyethane, benzaldehyde and 2-phenethyl acetate, changed above their odor thresholds under BFC, and those of decanoic acid, ethyl octanoate, ethyl decanoate and isoamyl acetate under NBFC. Of the twenty proteins involved in the metabolism of ethanol, acetaldehyde, acetoin, 2,3-butanediol, 1,1-diethoxyethane, benzaldehyde, organic acids and ethyl esters, only Adh2p, Ald4p, Cys4p, Fas3p, Met2p and Plb1p were detected under BFC and as many Acs2p, Ald3p, Cem1p, Ilv2p, Ilv6p and Pox1p, only under NBFC. Of the eight proteins involved in glycerol metabolism, Gut2p was detected only under BFC while Pgs1p and Rhr2p were under NBFC. Finally, of the five proteins involved in the metabolism of higher alcohols, Thi3p was present under BFC, and Aro8p and Bat2p were under NBFC.
authors.affiliation: Department of Microbiology, Severo Ochoa (C6) building, Agrifood Campus of International Excellence CeiA3, University of Cordoba, Ctra. N-IV-A mm 396, 14014 Córdoba, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Moreno-García
authors.affiliation: Department of Microbiology, Severo Ochoa (C6) building, Agrifood Campus of International Excellence CeiA3, University of Cordoba, Ctra. N-IV-A mm 396, 14014 Córdoba, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: García-Martínez
authors.affiliation: Department of Microbiology, Severo Ochoa (C6) building, Agrifood Campus of International Excellence CeiA3, University of Cordoba, Ctra. N-IV-A mm 396, 14014 Córdoba, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Millán
authors.affiliation: Department of Microbiology, Severo Ochoa (C6) building, Agrifood Campus of International Excellence CeiA3, University of Cordoba, Ctra. N-IV-A mm 396, 14014 Córdoba, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Mauricio
authors.affiliation: Department of Agricultural Chemistry, Marie Curie (C3) building, Agrifood Campus of International Excellence CeiA3, University of Córdoba, Ctra. N-IV-A, km 396, 14014 Cordoba, Spain. Electronic address: qe1movij@uco.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Moreno
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D002072:Butylene Glycols / Q000378:metabolism; D003651:Decanoates / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D055432:Metabolomics; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000378:metabolism; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D014920:Wine / Q000032:analysis
chemical_list: D000085:Acetates; D002072:Butylene Glycols; D003651:Decanoates; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; C026978:2,3-butylene glycol; C054590:2-phenylethyl acetate; C091960:ethyl decanoate; D005947:Glucose; D010626:Phenylethyl Alcohol; D005990:Glycerol; D019342:Acetic Acid
keywords: (PubChem CID: 176); (PubChem CID: 177); (PubChem CID: 240); (PubChem CID: 2969); (PubChem CID: 31276); (PubChem CID: 753); (PubChem CID: 7654); (PubChem CID: 7765); (PubChem CID: 7799); (PubChem CID: 8048); (PubChem CID: 702); 1,1-Diethoxyethane; 2-Phenethyl acetate; Acetaldehyde; Acetic acid; Benzaldehyde; Biofilm; Decanoic acid; Ethanol; Ethyl decanoate; Ethyl octanoate; Exometabolome; Flor yeast; Glycerol; Isoamyl acetate; Proteome; Saccharomyces cerevisiae