title: Transplanted Bone Marrow-Derived Cells Contribute to Human Adipogenesis.
abstract: Because human white adipocytes display a high turnover throughout adulthood, a continuous supply of precursor cells is required to maintain adipogenesis. Bone marrow (BM)-derived progenitor cells may contribute to mammalian adipogenesis; however, results in animal models are conflicting. Here we demonstrate in 65 subjects who underwent allogeneic BM or peripheral blood stem cell (PBSC) transplantation that, over the entire lifespan, BM/PBSC-derived progenitor cells contribute ∼10% to the subcutaneous adipocyte population. While this is independent of gender, age, and different transplantation-related parameters, body fat mass exerts a strong influence, with up to 2.5-fold increased donor cell contribution in obese individuals. Exome and whole-genome sequencing of single adipocytes suggests that BM/PBSC-derived progenitors contribute to adipose tissue via both differentiation and cell fusion. Thus, at least in the setting of transplantation, BM serves as a reservoir for adipocyte progenitors, particularly in obese subjects.
authors.affiliation: Department of Medicine (H7), Karolinska Institutet, 141 86 Stockholm, Sweden. Electronic address: mikael.ryden@ki.se.
authors.forename: Mikael
authors.initials: M
authors.lastname: Rydén
authors.affiliation: Department of Clinical Immunology, Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Uzunel
authors.affiliation: Department of Cell and Molecular Biology (C5), Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Hård
authors.affiliation: Science for Life Laboratory, Division of Gene Technology, Royal Institute of Technology (KTH), School of Biotechnology, 171 65 Stockholm, Sweden.
authors.forename: Erik
authors.initials: E
authors.lastname: Borgström
authors.affiliation: Department of Cell and Molecular Biology (C5), Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Jeff E
authors.initials: JE
authors.lastname: Mold
authors.affiliation: Department of Medicine (H7), Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Erik
authors.initials: E
authors.lastname: Arner
authors.affiliation: Department of Medicine (H7), Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Niklas
authors.initials: N
authors.lastname: Mejhert
authors.affiliation: Department of Medicine (H7), Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Andersson
authors.affiliation: Department of Medicine (H7), Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Widlund
authors.affiliation: Department of Laboratory Medicine (H5), Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Hassan
authors.affiliation: Department of Cell and Molecular Biology (C5), Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Christina V
authors.initials: CV
authors.lastname: Jones
authors.affiliation: Department of Cell and Molecular Biology (C5), Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Kirsty L
authors.initials: KL
authors.lastname: Spalding
authors.affiliation: Department of Clinical Immunology, Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Britt-Marie
authors.initials: BM
authors.lastname: Svahn
authors.affiliation: Science for Life Laboratory, Division of Gene Technology, Royal Institute of Technology (KTH), School of Biotechnology, 171 65 Stockholm, Sweden.
authors.forename: Afshin
authors.initials: A
authors.lastname: Ahmadian
authors.affiliation: Department of Cell and Molecular Biology (C5), Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Frisén
authors.affiliation: Institut Camille Jordan, CNRS UMR 5208, University of Lyon, 69622 Villeurbanne, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bernard
authors.affiliation: Department of Clinical Immunology, Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Mattsson
authors.affiliation: Department of Medicine (H7), Karolinska Institutet, 141 86 Stockholm, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Arner
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009765:Obesity / Q000378:metabolism; D036102:Peripheral Blood Stem Cell Transplantation; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D004247:DNA