title: MicroRNA-1297 regulates hepatocellular carcinoma cell proliferation and apoptosis by targeting EZH2.
abstract: In this study, we suggested the level of miR-1297 was downreguled in the human hepatocellular carcinoma compared to the normal cells. We demonstrate ectopic expression of miR-1297 could significantly suppress hepatocellular carcinoma cells proliferation and enhance the cell apoptosis. In vitro reporter assay suggested EZH2 is a direct target gene of miR-1297. Furthermore, knockdown of EZH2 have the same effect with miR-1297 overeexpression in hepatocellular carcinoma cells. These findings provide evidence that miR-1297 plays a key role in inhibition of the hepatocellular carcinoma cells proliferation, and enhancing cell apoptosis through targeting EZH2, and strongly suggest that ex ogenous miR-1297 may have therapeutic value in treating hepatocellular carcinoma.
authors.affiliation: Department of Infectious Diseases, Liaocheng People's Hospital Liaocheng 252000, Shandong, China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, Liaocheng People's Hospital Liaocheng 252000, Shandong, China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Gastroenterology, Jinan Central Hospital Affiliated to Shandong University Ji'nan 250000, Shandong, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Shu
authors.affiliation: Department of Infectious Diseases, Liaocheng People's Hospital Liaocheng 252000, Shandong, China.
authors.forename: Sikui
authors.initials: S
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D000071221:Enhancer of Zeste Homolog 2 Protein; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D063151:Polycomb Repressive Complex 2 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C578622:MIRN1297 microRNA, human; D035683:MicroRNAs; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
keywords: EZH2; hepatocellular carcinoma; miR-1297; miRNA