title: Impaired Development of Early Endometriotic Lesions in CD44 Knockout Mice.
abstract: Previous studies have shown endometrial cell (EC) CD44 and peritoneal mesothelial cell (PMC)-associated hyaluronan (hyaluronic acid [HA]) are involved in the attachment of endometrial stroma and epithelial cells to peritoneal mesothelium. Here we assess the CD44-HA interaction in the formation of the early endometriotic lesion using CD44(-/-) (knockout) mice. Using an established murine model and crossover technique, endometrial tissue from donor mice (wild type [WT] and CD44(-/-)) was used to induce endometriosis in recipient mice (WT and CD44(-/-)). Endometriotic lesions were visualized by fluorescent microscopy and confirmed by hematoxylin and eosin staining. Early endometriotic lesions were decreased when CD44(-/-) endometrium was placed in WT recipients and when WT endometrium was placed in CD44(-/-) recipients (P = .002). Early endometriotic lesions were also significantly decreased when both peritoneal and endometrial tissues lacked CD44 expression (P < .01). These studies demonstrate that both EC and PMC CD44 play a role in the development of early endometriotic lesion.
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA knudtson@uthscsa.edu.
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Knudtson
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Rajeshwar R
authors.initials: RR
authors.lastname: Tekmal
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Marlen Tellez
authors.initials: MT
authors.lastname: Santos
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Binkley
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Naveen
authors.initials: N
authors.lastname: Krishnegowda
authors.affiliation: Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Valente
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Schenken
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010532:Peritoneal Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010537:Peritoneum / Q000378:metabolism / Q000473:pathology*
chemical_list: C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid
keywords: CD44; endometriosis; endometriosis model; hyaluronic acid