title: OsSIDP366, a DUF1644 gene, positively regulates responses to drought and salt stresses in rice.
abstract: Domain of unknown function 1644 (DUF1644) is a highly conserved amino acid sequence motif present only in plants. Analysis of expression data of the family of DUF1644-containing genes indicated that they may regulate responses to abiotic stress in rice. Here we present our discovery of the role of OsSIDP366, a member of the DUF1644 gene family, in response to drought and salinity stresses in rice. Transgenic rice plants overexpressing OsSIDP366 showed enhanced drought and salinity tolerance and reduced water loss as compared to that in the control, whereas plants with downregulated OsSIDP366 expression levels using RNA interference (RNAi) were more sensitive to salinity and drought treatments. The sensitivity to abscisic acid (ABA) treatment was not changed in OsSIDP366-overexpressing plants, and OsSIDP366 expression was not affected in ABA-deficient mutants. Subcellular localization analysis revealed that OsSIDP366 is presented in the cytoplasmic foci that colocalized with protein markers for both processing bodies (PBs) and stress granules (SGs) in rice protoplasts. Digital gene expression (DGE) profile analysis indicated that stress-related genes such as SNAC1, OsHAK5 and PRs were upregulated in OsSIDP366-overexpressing plants. These results suggest that OsSIDP366 may function as a regulator of the PBs/SGs and positively regulate salt and drought resistance in rice.
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Chiming
authors.initials: C
authors.lastname: Guo
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Luo
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Guo
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: College of the Environment and Ecology, Xiamen University, Xiamen, 361102, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Guo
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, 29634, USA.
authors.forename: Liangjiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D009997:Osmotic Pressure; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D034622:RNA Interference; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid
keywords: Abiotic stress; DUF1644; processing bodies; rice; stress granules