title: A Homogenous Bioluminescent System for Measuring GTPase, GTPase Activating Protein, and Guanine Nucleotide Exchange Factor Activities.
abstract: GTPases play a major role in various cellular functions such as cell signaling, cell proliferation, cell differentiation, cytoskeleton modulation, and cell motility. Deregulation or mutation of these proteins has considerable consequences resulting in multiple pathological conditions. Targeting GTPases and its regulators has been challenging due to paucity of convenient assays. In this study, we describe a homogenous bioluminescent assay for monitoring the activities of GTPase and its immediate regulators: GTPase activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). Since Mg(2+) plays a critical role in influencing the affinity of GTPases with guanosine triphosphate/guanosine diphosphate (GTP/GDP) and the process of nucleotide exchange, manipulating Mg(2+) concentrations in the GTPase reaction buffer allows continuous progression of the GTPase cycle and faster hydrolysis of GTP. The assay relies on enzymatic conversion of GTP that remains after the GTPase reaction to ATP and detection of the generated ATP using the luciferin/luciferase combination. The GTPase/GAP/GEF-Glo assay system enables monitoring of GTPase, GAP-stimulated GTPase, GAP, and GEF activities. The system can also be used to analyze these proteins when expressed in cells as fusion proteins by performing the assay in a pulldown format. The assays showed minimal false hits upon testing for compound interference using the library of pharmacologically active compounds and its robustness was demonstrated by a high Z'-factor of 0.93 and CV of 2.2%. The assay system has a high dynamic range, formatted in a convenient add-mix-read, and applicable to high-throughput screening.
authors.affiliation: 1 Research and Development , Promega Corporation, Madison, Wisconsin.
authors.forename: Subhanjan
authors.initials: S
authors.lastname: Mondal
authors.affiliation: 1 Research and Development , Promega Corporation, Madison, Wisconsin.
authors.forename: Kevin
authors.initials: K
authors.lastname: Hsiao
authors.affiliation: 1 Research and Development , Promega Corporation, Madison, Wisconsin.|2 Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health , Madison, Wisconsin.
authors.forename: Said A
authors.initials: SA
authors.lastname: Goueli
mesh_terms: D004789:Enzyme Activation / Q000502:physiology; D020558:GTP Phosphohydrolases / Q000032:analysis* / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000032:analysis* / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D020558:GTP Phosphohydrolases