title: Auricularia auricular-judaepolysaccharide attenuates lipopolysaccharide-induced acute lung injury by inhibiting oxidative stress and inflammation.
abstract: Auricularia auricular-judaepolysaccharide (AAP) has shown a variety of pharmacological properties. In the present study, the role of AAP in acute lung injury (ALI) induced by lipopolysaccharide (LPS) was analyzed in rats to further explore the possible underlying mechanisms. Adult Sprague-Dawley rats were randomly assigned into the control, AAP, LPS and LPS plus AAP groups. Rats were injected with LPS (10 mg/kg, intraperitoneal) to induce ALI. Rats in the LPS plus AAP group were treated with AAP for 7 days before the induction of ALI. The protein concentration in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by the wet/dry (W/D) weight ratio. The myeloperoxidase (MPO) activity and malondialdehyde (MDA) level were assayed by MPO and MDA kits, respectively. The levels of inflammatory mediators, tumor necrosis factor-α (TNF-α) and interleukin (IL)-6, were assayed by the enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed by hematoxylin and eosin staining. The data showed that treatment with AAP significantly improved LPS-induced lung pathological changes, attenuated protein concentration in the BALF, inhibited MPO activity and reduced the MDA level and lung W/D weight ratio. AAP also inhibited the release of TNF-α and IL-6 in blood. The results indicated that AAP has a protective effect on LPS-induced ALI in rats.
authors.affiliation: Clinical Medical College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: L I
authors.initials: LI
authors.lastname: Zhuan-Yun
authors.affiliation: Laboratory of Functional Experiment, Preclinical Medicine College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xue-Ping
authors.affiliation: Clinical Medical College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Bin
authors.affiliation: Clinical Medical College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Ha Nizaier
authors.initials: HN
authors.lastname: Reheman
authors.affiliation: Clinical Medical College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Gao
authors.initials: G
authors.lastname: Yang
authors.affiliation: Laboratory of Functional Experiment, Preclinical Medicine College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Sun
authors.initials: S
authors.lastname: Zhan
authors.affiliation: Laboratory of Functional Experiment, Preclinical Medicine College, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: M A
authors.initials: MA
authors.lastname: Qi
keywords: Auricularia auricular-judae polysaccharide; acute lung injury; inflammation; lipopolysaccharide; oxidative stress