title: Role of bone marrow mesenchymal stem cells in L-arg-induced acute pancreatitis: effects and possible mechanisms.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) have shown an obvious protective effect on systemic inflammation. The purpose of this study is to assess the effect and possible mechanism of bone marrow MSCs (bmMSCs) on acute pancreatitis (AP).
  
  
  METHODS
  BmMSCs of SD rats were isolated and cultured in vitro. L-Arginine-induced acute pancreatitis was used as AP model in vivo. Pancreatic injury was assessed by serum amylase, lipase, cytokines and pancreatic histology. RT-PCR was applied to investigate mRNA expression of pancreas tissue. Western-blot and immunohistochemistry (IHC) were applied to test the role of NF-κB p65 signaling pathway. Tracking and Positioning of CM-Dil labeled bmMSCs in vivo was further studied.
  
  
  RESULTS
  Treatment with bmMSCs attenuated acute pancreatic injury and AP-associated lung injury obviously, with decreased serum IL-1β, IL-6, TNF-α, down-regulated expressions of IL-1α, IL-6, TNFα in pancreas tissue and reduced nuclear translocation of NF-κB p65 in AP. Localization of bmMSCs in vivo was due to being passively trapped in related organs, but not actively homing to inflammatory sites of pancreas during the early phase of AP.
  
  
  CONCLUSIONS
  Taken together, the results showed that bmMSCs played a protective role in AP in many aspects, which might protect against experimental pancreatitis partly by regulating release of inflammatory cytokines by an exocrine secretion.
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Soochow University Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Guojian
authors.initials: G
authors.lastname: Yin
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors.forename: Guoyong
authors.initials: G
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wan
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors.forename: Ge
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Soochow University Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cang
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Xing
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Zhabei District, Shanghai 200072, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Soochow University Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine 100 Haining Road, Hongkou District, Shanghai 200080, People's Republic of China.
authors.forename: Xingpeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001120:Arginine / Q000633:toxicity; D015153:Blotting, Western; D001854:Bone Marrow Cells; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D010195:Pancreatitis / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001120:Arginine
keywords: Mesenchymal stem cells; acute pancreatitis