title: Effects of 5-hydroxytryptophan and m-hydroxybenzylhydrazine associated to Lactobacillus spp. on the humoral response of broilers challenged with Salmonella Enteritidis.
abstract: This study investigates the effects of different doses of serotonin, its precursor 5-hydroxytry-ptophan (5HTP), and m-hydroxybenzylhydrazine inhibitor (NSD1015), administered via intraperitoneal for 5 consecutive days, on behavior and average body weight of broilers. We also measured the humoral immune response and quantification of Salmonella Enteritidis in broilers chickens that received the drugs evaluated and a Lactobacillus pool. The study was divided into 3 experiments: Experiment 1--administration of pharmaceuticals with choice of dosage; Experiment 2--administration of pharmaceuticals and a Lactobacillus pool in birds that were not challenged with S. Enteritidis, and Experiment 3--administration of pharmaceuticals and a Lactobacillus pool in birds challenged with S. Enteritidis. The ELISA was used to scan dosages of intestinal IgA and serum IgY. We used colony-forming units to quantify S. Enteritidis. The concentrations of IgA and IgY did not show significant differences (P>0.05) in Experiment 2. In Experiment 3, NSD1015 associated with Lactobacillus determined higher IgA concentrations, promoting greater stimulus to the immune system than 5HTP. Regarding quantification of S. Enteritidis in the cecal content of birds, 5HTP associated to Lactobacillus determined the smallest number of bacteria, showing possible interaction of 5-hydroxytryptophan and Lactobacillus spp. with the immune system of broiler chickens.
authors.affiliation: Sao Paulo State University (UNESP), College of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil tais.donato@yahoo.com.br.
authors.forename: T C
authors.initials: TC
authors.lastname: Donato
authors.affiliation: Londrina State University (UEL), Department of Preventive Veterinary Medicine, Laboratory of Avian Medicine, Londrina, PR, Brazil.
authors.forename: A A S
authors.initials: AA
authors.lastname: Baptista
authors.affiliation: Sao Paulo State University (UNESP), College of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil.
authors.forename: K C O D
authors.initials: KC
authors.lastname: Garcia
authors.affiliation: Sao Paulo State University (UNESP), College of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil.
authors.forename: B D
authors.initials: BD
authors.lastname: Smaniotto
authors.affiliation: Sao Paulo State University (UNESP), College of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Okamoto
authors.affiliation: Sao Paulo State University (UNESP), College of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil.
authors.forename: J L
authors.initials: JL
authors.lastname: Sequeira
authors.affiliation: Sao Paulo State University (UNESP), College of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil.
authors.forename: R L
authors.initials: RL
authors.lastname: Andreatti Filho
mesh_terms: D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000818:Animals; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D015169:Colony Count, Microbial / Q000662:veterinary; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D006834:Hydrazines / Q000494:pharmacology; D056724:Immunity, Humoral / Q000187:drug effects*; D007778:Lactobacillus / Q000737:chemistry*; D008297:Male; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000502:physiology; D012702:Serotonin Antagonists / Q000494:pharmacology*
chemical_list: D006834:Hydrazines; D012702:Serotonin Antagonists; C004297:3-hydroxybenzylhydrazine; D006916:5-Hydroxytryptophan
keywords: Lactobacillus spp.; Salmonella Enteritidis; broilers; immune response; serotonin