title: Carbonic anhydrase 2-like and Na⁺-K⁺-ATPase α gene expression in medaka (Oryzias latipes) under carbonate alkalinity stress.
abstract: High carbonate alkalinity is one of the major stress factors for living organisms in saline-alkaline water areas. Acute and chronic effects of carbonate alkalinity on expression of two genes, carbonic anhydrase 2-like (CA2-like) and Na(+)-K(+)-ATPase α subunit (NKA-α) mRNA in medaka (Oryzias latipes) were evaluated to better understand the responses important for coping with a carbonate alkalinity stress. In the acute exposure experiment, the expression of CA2-like and NKA-α mRNA in the gill and kidney of medaka were examined from 0 h to 7 days exposed to 30.4 mM carbonate alkalinity water. Exposure to high carbonate alkalinity resulted in a transitory alkalosis, followed by a transient increase in gill and kidney CA2-like and NKA-α mRNA expression. In the chronic exposure experiment, the expression of these two genes was examined in the gill and kidney at 50 days post-exposure to six different carbonate alkalinity concentrations ranging from 1.5 to 30.4 mM. Gill and kidney CA2-like mRNA levels in 30.4 mM were approximately 10 and 30 times higher than that of the control (1.5 mM), respectively. Less differences were found in NKA-α expression in the 50-days exposure. The results indicate that when transferred to high carbonate alkalinity water, a transitory alkalosis may occur in medaka, followed by compensatory acid-base and ion regulatory responses. Thus, CA2-like and NKA-α are at least two of the important factors that contribute to the regulation of alkalinity stress.
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 20090, China. yaozongli@hotmail.com.
authors.forename: Zongli
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 20090, China. qifanglai@163.com.
authors.forename: Qifang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 20090, China. haozhuoran@126.com.
authors.forename: Zhuoran
authors.initials: Z
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China. chenling@tongji.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 20090, China. lintt@eastfishery.ac.cn.
authors.forename: Tingting
authors.initials: T
authors.lastname: Lin
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 20090, China. zhoukai69@126.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 20090, China. dhswh99@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002254:Carbonates / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D009990:Oryzias / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D002254:Carbonates; D012333:RNA, Messenger; D002256:Carbonic Anhydrases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: --ATPase; Alkalinity stress; Carbonic anhydrase; Oryzias latipes; Quantitative real-time PCR