title: Impact of CAD-deficiency in flax on biogas production.
abstract: Global warming and the reduction in our fossil fuel reservoir have forced humanity to look for new means of energy production. Agricultural waste remains a large source for biofuel and bioenergy production. Flax shives are a waste product obtained during the processing of flax fibers. We investigated the possibility of using low-lignin flax shives for biogas production, specifically by assessing the impact of CAD deficiency on the biochemical and structural properties of shives. The study used genetically modified flax plants with a silenced CAD gene, which encodes the key enzyme for lignin synthesis. Reducing the lignin content modified cellulose crystallinity, improved flax shive fermentation and optimized biogas production. Chemical pretreatment of the shive biomass further increased biogas production efficiency.
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630, Wrocław, Poland. magdalena.wrobel-kwiatkowska@up.wroc.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wróbel-Kwiatkowska
authors.affiliation: Department of Biotransformation, Faculty of Biotechnology, University of Wrocław, F. Joliot-Curie 14a, 50-383, Wrocław, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Jabłoński
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63, 51-147, Wrocław, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Szperlik
authors.affiliation: Department of Bioorganic Chemistry, Faculty of Engineering and Economics, Institute of Chemistry and Food Technology, Wrocław University of Economics, Komandorska 118/120, 50-345, Wrocław, Poland.
authors.forename: Lucyna
authors.initials: L
authors.lastname: Dymińska
authors.affiliation: Department of Biotransformation, Faculty of Biotechnology, University of Wrocław, F. Joliot-Curie 14a, 50-383, Wrocław, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Łukaszewicz
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630, Wrocław, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rymowicz
authors.affiliation: Department of Bioorganic Chemistry, Faculty of Engineering and Economics, Institute of Chemistry and Food Technology, Wrocław University of Economics, Komandorska 118/120, 50-345, Wrocław, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Hanuza
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63, 51-147, Wrocław, Poland.|Department of Genetics, Plant Breeding and Seed Production, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 24A, 53-363, Wrocław, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Szopa
mesh_terms: D000429:Alcohol Oxidoreductases / Q000172:deficiency*; D056804:Biofuels; D002482:Cellulose / Q000032:analysis; D019597:Flax / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008031:Lignin / Q000032:analysis; D010368:Pectins / Q000032:analysis; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D056804:Biofuels; D010368:Pectins; D002482:Cellulose; D008031:Lignin; D000429:Alcohol Oxidoreductases; C018656:cinnamyl alcohol dehydrogenase
keywords: Biogas production; CAD gene; FTIR (Fourier transform infrared spectroscopy); Shives; Transgenic flax