title: Amniotic epithelial cells promote wound healing in mice through high epithelialization and engraftment.
abstract: Although human amniotic epithelial cells (AMEs) are an attractive source of stem cells, their therapeutic potential in wound healing has not been fully investigated. We evaluated the therapeutic potential of AMEs for wound healing. Real-time PCR showed that the epithelialization growth factors epidermal growth factor (EGF), platelet-derived growth factor (PDGF)-B and chemotactic factors interleukin-8 (IL-8 or CXCL8) and neutrophil-activating protein-2 (NAP-2 or CXCL7) were upregulated in AMEs compared with adipose-derived mesenchymal stem cells (ADMs). In vitro scratch wound assays revealed that AME-derived conditioned medium substantially accelerated wound closure. Wounds in NOD/SCID mice were created by skin excision, followed by AME transplantation. AMEs implantation significantly accelerated wound healing and increased cellularity and re-epithelialization. Transplanted AMEs exhibited high engraftment rates and expressed keratinocyte-specific proteins and cytokeratin in the wound area, suggesting direct benefits for cutaneous closure. Taken together, these data indicate that AMEs possess therapeutic capability for wound healing through the secretion of epithelialization growth factors and enhanced engraftment properties. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Cardiology, Fourth Affiliated Hospital of Harbin Medical University, People's Republic of China.
authors.forename: Enze
authors.initials: E
authors.lastname: Jin
authors.affiliation: Departments of Obstetrics and Gynaecology, Soonchunhyang University College of Medicine, Bucheon, Republic of Korea.
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Family Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
authors.forename: Seongho
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Cardiology, Fourth Affiliated Hospital of Harbin Medical University, People's Republic of China.|Department of Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea.
authors.forename: Sung-Whan
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000650:Amnion / Q000166:cytology / Q000378:metabolism; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D064593:Heterografts; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D062326:Re-Epithelialization; D014947:Wounds and Injuries / Q000378:metabolism / Q000628:therapy
chemical_list: D016207:Cytokines
keywords: amniotic epithelial cell; engraftment; epithelialization; growth factor; wound healing