title: Protective role of Diospyros lotus on cisplatin-induced changes in sperm characteristics, testicular damage and oxidative stress in rats.
abstract: The aim of this study was to investigate the protective effect of Diospyros lotus (DL) on cisplatin (CP)-induced testicular damage in male rats. Twenty-eight male rats were randomly divided into four groups: group 1 - control, given isotonic saline solution; group 2 - CP 7 mg kg(-1) given intraperitoneally as single dose; group 3 - DL 1000 mg kg(-1) per day given orally for 10 days; group 4 - CP and DL given together at the same doses. CP caused a significant increase in thiobarbituric acid-reactive substances (TBARS) level and a significant decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) levels in rats testis tissues compared to the control group. CP caused a significant increase in lipid peroxidation in testis tissues compared to the control group, whereas DL led to a significant increase in SOD and GSH levels. However, there were no statistically significant changes in GPx and CAT levels. In addition, serum testosterone levels, sperm concentration and sperm motility were significantly decreased, but abnormal sperm rate and histological changes were increased with CP. However, these effects of CP on sperm parameters, histological changes and the tissue weights were eliminated by DL treatment. In conclusion, our study showed that the reproductive toxicity caused by CP may be prevented by DL treatment.
authors.affiliation: Department of Nutrition and Dietetics, School of Healthy Sciences, Artvin Coruh University, Artvin, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Saral
authors.affiliation: Department of Nutrition and Dietetics, School of Healthy Sciences, Artvin Coruh University, Artvin, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Ozcelik
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Cetin
authors.affiliation: Department of Nutrition and Dietetics, School of Healthy Sciences, Artvin Coruh University, Artvin, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Saral
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Basak
authors.affiliation: Department of Obstetric and Gynecology, Faculty of Veterinary Medicine, Firat University, Elazıg, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Aydın
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Ciftci
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D029788:Diospyros; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D002945:Cisplatin
keywords: Cisplatin; Diospyros lotus; oxidative stress; testicular damage