title: Age-related changes in platelet function are more profound in women than in men.
abstract: Age is a risk factor for cardiovascular disease (CVD), however the effect of age on platelet function remains unclear. Ideally, platelet function should be assayed under flow and shear conditions that occur in vivo. Our study aimed to characterise the effect of age on platelet translocation behaviour using a novel flow-based assay that measures platelet function in less than 200 μl of blood under conditions of arterial shear. Blood from males (n = 53) and females (n = 56), ranging in age from 19-82 and 21-70 respectively were perfused through custom-made parallel plate flow chambers coated with immobilised human von Willebrand Factor (VWF) under arterial shear (1,500 s(-1)). Platelet translocation behaviour on VWF was recorded by digital-image microscopy and analysed. The study showed that aging resulted in a significant decrease in the number of platelet tracks, translocating platelets and unstable platelet interactions with VWF. These age related changes in platelet function were more profound in women than in men indicating that age and gender significantly impacts on platelet interactions with VWF.
authors.affiliation: 1] Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland [2] Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cowman
authors.affiliation: 1] Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland [2] Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
authors.forename: Eimear
authors.initials: E
authors.lastname: Dunne
authors.affiliation: Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
authors.forename: Irene
authors.initials: I
authors.lastname: Oglesby
authors.affiliation: Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
authors.forename: Barry
authors.initials: B
authors.lastname: Byrne
authors.affiliation: Irish Centre for High-end Computing, National University Ireland, Galway, Ireland.
authors.forename: Adam
authors.initials: A
authors.lastname: Ralph
authors.affiliation: Irish Centre for High-end Computing, National University Ireland, Galway, Ireland.
authors.forename: Bruno
authors.initials: B
authors.lastname: Voisin
authors.affiliation: Royal College of Surgeons in Ireland, Rotunda Hospital, Dublin, Ireland.
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Müllers
authors.affiliation: Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Ricco
authors.affiliation: 1] Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland [2] Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
authors.forename: Dermot
authors.initials: D
authors.lastname: Kenny
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D001792:Blood Platelets / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012737:Sex Factors; D055815:Young Adult