title: How a Small Double-Stranded Trick Can Mislead Sanger Sequencing.
abstract: Notwithstanding the arrival of "third-generation sequencing," Sanger sequencing, developed in 1980, is still the most accurate and used method for sequencing, although on a smaller scale. It is a powerful resource for studying sequences and discovering polymorphisms and genes, as well as regulatory elements. There has already been described a wide range of possible problems with this very sensitive and accurate technology. Here, we show that a specific event, related to genomes rich in repetitive sequences, can mislead operators working with Sanger sequencing.
authors.affiliation: Institute of Biosciences and Bioresources, National Research Council, Bari, Italy.
authors.forename: Pasquale Luca
authors.initials: PL
authors.lastname: Curci
authors.affiliation: Institute of Biosciences and Bioresources, National Research Council, Bari, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sonnante
mesh_terms: D016477:Artifacts; D018744:DNA, Plant / Q000235:genetics; D016678:Genome; D055029:Inverted Repeat Sequences; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics
chemical_list: D018744:DNA, Plant
keywords: chromatograms; inverted repeats; problems