title: Expression of the μ, κ, and δ-opioid receptors and tyrosine hydroxylase in MN9D cells.
abstract: Dopaminergic neurons are suggested to be a critical physiopathology substrate for addiction disorders. It is not well known whether the clonal mesencephalic dopaminergic cell line MN9D cells can be applied to study morphine addiction. Immunofluorescence staining and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect protein and mRNA expression of the μ, κ, and δ-opioid receptors in MN9D cells. Immunofluorescence staining of TH was applied to quantify the number of dopaminergic neurons. The results showed that the μ, κ, and δ-receptors were all expressed in MN9D cells, and the number of TH-positive cells was significantly greater in the MN9D cells than SH-SY5Y cells. The data suggest that MN9D cells can be used as an in vitro models in future studies to explore the mechanisms of morphine addiction related to dopaminergic neurons.
authors.affiliation: Institute of Clinical Medicine, Hebei Medical University Hebei, China.
authors.forename: Pengxiang
authors.initials: P
authors.lastname: Tian
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Shi
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Ma
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cong
authors.affiliation: Institute of Basic Medicine, Hebei Medical University Hebei, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Li
mesh_terms: D002460:Cell Line; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D017465:Receptors, Opioid, delta / Q000235:genetics / Q000378:metabolism*; D017473:Receptors, Opioid, kappa / Q000235:genetics / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000235:genetics / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D014446:Tyrosine 3-Monooxygenase
keywords: MN9D cells; SH-SY5Y cells; opioid receptors; tyrosine hydroxylase