title: β-Radiation Stress Responses on Growth and Antioxidative Defense System in Plants: A Study with Strontium-90 in Lemna minor.
abstract: In the following study, dose dependent effects on growth and oxidative stress induced by β-radiation were examined to gain better insights in the mode of action of β-radiation induced stress in plant species. Radiostrontium (⁹⁰Sr) was used to test for β-radiation induced responses in the freshwater macrophyte Lemna minor. The accumulation pattern of 90Sr was examined for L. minor root and fronds separately over a seven-day time period and was subsequently used in a dynamic dosimetric model to calculate β-radiation dose rates. Exposing L. minor plants for seven days to a ⁹⁰Sr activity concentration of 25 up to 25,000 kBq·L⁻¹ resulted in a dose rate between 0.084 ± 0.004 and 97 ± 8 mGy·h⁻¹. After seven days of exposure, root fresh weight showed a dose dependent decrease starting from a dose rate of 9.4 ± 0.5 mGy·h⁻¹. Based on these data, an EDR10 value of 1.5 ± 0.4 mGy·h⁻¹ was estimated for root fresh weight and 52 ± 17 mGy·h⁻¹ for frond fresh weight. Different antioxidative enzymes and metabolites were further examined to analyze if β-radiation induces oxidative stress in L. minor.
authors.affiliation: SCK•CEN, Belgian Nuclear Research Centre, Boeretang 200, 2400 Mol, Belgium. avhoeck@sckcen.be.|Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium. avhoeck@sckcen.be.
authors.forename: Arne
authors.initials: A
authors.lastname: Van Hoeck
authors.affiliation: SCK•CEN, Belgian Nuclear Research Centre, Boeretang 200, 2400 Mol, Belgium. nhoreman@sckcen.be.|Centre for Environmental Research, University of Hasselt, Universiteitslaan 1, 3590 Diepenbeek, Belgium. nhoreman@sckcen.be.
authors.forename: Nele
authors.initials: N
authors.lastname: Horemans
authors.affiliation: SCK•CEN, Belgian Nuclear Research Centre, Boeretang 200, 2400 Mol, Belgium. mvhees@sckcen.be.
authors.forename: May
authors.initials: M
authors.lastname: Van Hees
authors.affiliation: SCK•CEN, Belgian Nuclear Research Centre, Boeretang 200, 2400 Mol, Belgium. rnauts@sckcen.be.
authors.forename: Robin
authors.initials: R
authors.lastname: Nauts
authors.affiliation: Veterinary Sciences, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium. dries.knapen@uantwerpen.be.
authors.forename: Dries
authors.initials: D
authors.lastname: Knapen
authors.affiliation: SCK•CEN, Belgian Nuclear Research Centre, Boeretang 200, 2400 Mol, Belgium. hvandenh@sckcen.be.
authors.forename: Hildegarde
authors.initials: H
authors.lastname: Vandenhove
authors.affiliation: Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium. ronny.blust@uantwerpen.be.
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D029064:Araceae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D001610:Beta Particles; D055442:Metabolome / Q000528:radiation effects; D011874:Radiometry; D013312:Stress, Physiological / Q000187:drug effects / Q000528:radiation effects*; D013326:Strontium Radioisotopes / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D013326:Strontium Radioisotopes
keywords: Lemna minor; abiotic stress; dosimetry; oxidative stress; radiation responses; strontium-90