title: CMV-induced embryonic mouse organ of corti dysplasia: Network architecture of dysfunctional lateral inhibition.
abstract: BACKGROUND
  Congenital cytomegalovirus infection is the major nongenetic cause of sensorineural hearing loss at birth and beyond. Among other pathologies, there is a striking dysplasia/hyperplasia of organ of Corti hair and supporting cells.
  
  
  METHODS
  Using an in vitro embryonic mouse model of cytomegalovirus-induced cochlear teratogenesis that mimics the known human pathology, and functional signaling network modeling, we tested the hypothesis that cytomegalovirus disrupts the highly ordered organ of Corti hair and supporting cells pattern by dysregulating Notch and Fgfr3, their cognate ligands and downstream effectors.
  
  
  RESULTS
  Several novel emergent properties of the critical lateral inhibition subnetwork became apparent. The subnetwork has classic small-world properties such as short paths between most gene pairs, few long-distance links, and considerable clustering. Concomitantly, the calculated probability that our specific gene expression dataset is from dysplastic organs of Corti is highly significant (p < 1 × 10(-12) ). Furthermore, we determined that the subnetwork has a highly heterogeneous scale-free topology in which the highly linked genes (hubs), Notch and Fgfr3, play a central role in mediating interactions among the less linked genes.
  
  
  CONCLUSIONS
  This phenomenon has important biologic and therapeutic implications.
authors.affiliation: Laboratory Developmental Genetics, University of Southern California, Los Angeles, California.
authors.forename: Michael
authors.initials: M
authors.lastname: Melnick
authors.affiliation: Laboratory Developmental Genetics, University of Southern California, Los Angeles, California.
authors.forename: Tina
authors.initials: T
authors.lastname: Jaskoll
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000472:pathogenicity*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009925:Organ of Corti / Q000473:pathology*; D011247:Pregnancy
keywords: CMV; Fgfr3; Notch; hair cells; network architecture; organ of Corti