title: Immunoproteomic Identification of p29 Antigen as the Elongation Factor-1α of Leishmania mexicana.
abstract: Previously, we identified five Leishmania mexicana antigens reacting with antibodies from cutaneous leishmaniasis patients, designated on the basis of their molecular weights as p26 (pI 7.8), p27 (pI 8.1), p28 (pI 8.6), p29 (pI 8.5), and p31 (pI 9.0). Among these antigens, p29 was most strongly recognized by the antibodies. Thereafter, p29 was identified as elongation factor-1α (EF-1α) of Leishmania mexicana through mass spectrometry analysis and western blot using a commercial antibody that reacted with EF-1α from different species. Our results showed that the p29 antigen of Leishmania mexicana is EF-1α.
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: Zayda L
authors.initials: ZL
authors.lastname: Piedra-Quintero
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: Annete I
authors.initials: AI
authors.lastname: Apodaca-Medina
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: Evangelina
authors.initials: E
authors.lastname: Beltrán-López
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: Claudia R
authors.initials: CR
authors.lastname: León-Sicairos
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: Jeanett
authors.initials: J
authors.lastname: Chávez-Ontiveros
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: José G
authors.initials: JG
authors.lastname: Rendón-Maldonado
authors.affiliation: 2 Departamento de Biomedicina Molecular, CINVESTAV, Zacatenco, DF, México .
authors.forename: Vianney F
authors.initials: VF
authors.lastname: Ortiz-Navarrete
authors.affiliation: 1 Laboratorio de Biomedicina Molecular, CA Biotecnología Biomédica, Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria , Culiacán, Sinaloa, México .
authors.forename: Héctor S
authors.initials: HS
authors.lastname: López-Moreno
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D020648:Peptide Elongation Factor 1 / Q000276:immunology*; D040901:Proteomics
chemical_list: D000953:Antigens, Protozoan; D020648:Peptide Elongation Factor 1
keywords: Antigen; Cutaneous leishmaniasis; EF-1α; Immunoproteomic; Leishmania mexicana