title: Effects of β-(1→3,1→6)-D-glucan and density of diets on the blood profiles of immunologically challenged weaned piglets.
abstract: An experiment was conducted to evaluate the effects of two levels of the β-(1→3,1→6)-D-glucan (0 and 500 ppm) from yeast Saccharomyces cerevisiae and two levels of energy (3300 and 3450 kcal ME kg(-1)) on the hematological, immunological and, biochemical profiles of thirty-six 21-days-old weaned piglets, challenged with 150 μg kg(-1) of BW lipopolysaccharide (LPS) from Escherichia coli serotype 055:B5. The experimental design was a randomized complete block design in a 2×2 factorial arrangement with nine replicates per treatment and, one animal per experimental unit. The data were analyzed in accordance with the multivariate analysis procedure of SAS and, the treatment means of parametric and non-parametric data were compared by Bonferroni's test (P<0.05) and, by Dunn's test (P<0.05), respectively. The data of the blood profiles of alanine aminotransferase, alkaline phosphatase and, creatinine showed that LPS did not cause kidney or liver damage in the animals. The addition of beta-glucan in the diets did not prove the robustness of its effect and biological relevance when provided with low nutrient-density. However, its addition combined with the high-nutrient-density diets showed less marked hypoglobulinemia in piglets, which may have contributed to the decreasing of the synthesis of inflammatory mediators.
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine and Animal Science, UNESP - São Paulo State University, Botucatu Campus, P.O. Box 560, Zip Code 18618-970, Brazil. Electronic address: dib_saleh@yahoo.com.br.
authors.forename: Mayra A D
authors.initials: MA
authors.lastname: Saleh
authors.affiliation: Institute of Agricultural Sciences and Technologies, Federal University of Mato Grosso, Rondonópolis Campus, Zip Code 78735-902, Brazil.
authors.forename: Alessandro B
authors.initials: AB
authors.lastname: Amorim
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine and Animal Science, UNESP - São Paulo State University, Botucatu Campus, P.O. Box 560, Zip Code 18618-970, Brazil.
authors.forename: Henrique A T
authors.initials: HA
authors.lastname: Grecco
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine and Animal Science, UNESP - São Paulo State University, Botucatu Campus, P.O. Box 560, Zip Code 18618-970, Brazil.
authors.forename: Dirlei A
authors.initials: DA
authors.lastname: Berto
authors.affiliation: Department of Biostatistics, Biosciences Institute, UNESP - São Paulo State University, Botucatu Campus, P.O. Box 510, Zip Code18618-970, Brazil.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Padovani
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine and Animal Science, UNESP - São Paulo State University, Botucatu Campus, P.O. Box 560, Zip Code 18618-970, Brazil.
authors.forename: Ricardo de O
authors.initials: Rde O
authors.lastname: Orsi
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine and Animal Science, UNESP - São Paulo State University, Botucatu Campus, P.O. Box 560, Zip Code 18618-970, Brazil.
authors.forename: Marcos L P
authors.initials: ML
authors.lastname: Tse
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000097:blood / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D001774:Blood Chemical Analysis; D004909:Erythrocyte Indices; D005260:Female; D007114:Immunization; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D013552:Swine; D014886:Weaning; D047071:beta-Glucans / Q000008:administration & dosage*
chemical_list: D000906:Antibodies; D015415:Biomarkers; D008070:Lipopolysaccharides; D047071:beta-Glucans
keywords: Endotoxin; Hematology; Immunomodulation