title: The Genetic Relationship between Leishmania aethiopica and Leishmania tropica Revealed by Comparing Microsatellite Profiles.
abstract: BACKGROUND
  Leishmania (Leishmania) aethiopica and L. (L.) tropica cause cutaneous leishmaniases and appear to be related. L. aethiopica is geographically restricted to Ethiopia and Kenya; L. tropica is widely dispersed from the Eastern Mediterranean, through the Middle East into eastern India and in north, east and south Africa. Their phylogenetic inter-relationship is only partially revealed. Some studies indicate a close relationship. Here, eight strains of L. aethiopica were characterized genetically and compared with 156 strains of L. tropica from most of the latter species' geographical range to discern the closeness.
  
  
  RESULTS
  Twelve unlinked microsatellite markers previously used to genotype strains of L. tropica were successfully applied to the eight strains of L. aethiopica and their microsatellite profiles were compared to those of 156 strains of L. tropica from various geographical locations that were isolated from human cases of cutaneous and visceral leishmaniasis, hyraxes and sand fly vectors. All the microsatellite profiles were subjected to various analytical algorithms: Bayesian statistics, distance-based and factorial correspondence analysis, revealing: (i) the species L. aethiopica, though geographically restricted, is genetically very heterogeneous; (ii) the strains of L. aethiopica formed a distinct genetic cluster; and (iii) strains of L. aethiopica are closely related to strains of L. tropica and more so to the African ones, although, by factorial correspondence analysis, clearly separate from them.
  
  
  CONCLUSIONS
  The successful application of the 12 microsatellite markers, originally considered species-specific for the species L. tropica, to strains of L. aethiopica confirmed the close relationship between these two species. The Bayesian and distance-based methods clustered the strains of L. aethiopica among African strains of L. tropica, while the factorial correspondence analysis indicated a clear separation between the two species. There was no correlation between microsatellite profiles of the eight strains of L. aethiopica and the type of leishmaniasis, localized (LCL) versus diffuse cutaneous leishmaniasis (DCL), displayed by the human cases.
authors.affiliation: Institute of Microbiology and Hygiene, Charité-University Medicine, Berlin, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Krayter
authors.affiliation: Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: Lionel F
authors.initials: LF
authors.lastname: Schnur
authors.affiliation: Institute of Microbiology and Hygiene, Charité-University Medicine, Berlin, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schönian
mesh_terms: D001499:Bayes Theorem; D016054:DNA, Protozoan / Q000032:analysis*; D005002:Ethiopia; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D007895:Leishmania tropica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000469:parasitology; D018895:Microsatellite Repeats; D010802:Phylogeny; D058974:Phylogeography
chemical_list: D016054:DNA, Protozoan