title: The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms.
abstract: The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana. We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
authors.forename: Rubén Gustavo
authors.initials: RG
authors.lastname: Schlaen
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
authors.forename: Estefanía
authors.initials: E
authors.lastname: Mancini
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
authors.forename: Sabrina Elena
authors.initials: SE
authors.lastname: Sanchez
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
authors.forename: Soledad
authors.initials: S
authors.lastname: Perez-Santángelo
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
authors.forename: Matías L
authors.initials: ML
authors.lastname: Rugnone
authors.affiliation: Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom;
authors.forename: Craig G
authors.initials: CG
authors.lastname: Simpson
authors.affiliation: Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom; Plant Sciences, University of Dundee at James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom;
authors.forename: John W S
authors.initials: JW
authors.lastname: Brown
authors.affiliation: Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
authors.forename: Ariel
authors.initials: A
authors.lastname: Chernomoretz
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina; myanovsky@leloir.org.ar.
authors.forename: Marcelo J
authors.initials: MJ
authors.lastname: Yanovsky
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D002940:Circadian Rhythm; D016000:Cluster Analysis; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D055106:Genome-Wide Association Study; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics; D018515:Plant Leaves / Q000502:physiology; D012342:RNA, Small Nuclear / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D055532:SMN Complex Proteins / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D017381:Spliceosomes / Q000502:physiology; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C000602317:GEMIN2 protein, Arabidopsis; C108381:GEMIN2 protein, human; D009419:Nerve Tissue Proteins; D012342:RNA, Small Nuclear; D016601:RNA-Binding Proteins; D055532:SMN Complex Proteins
keywords: Arabidopsis; GEMIN2; alternative splicing; circadian rhythms; spliceosome assembly