title: Nanostructured multilayer polyelectrolyte films with silver nanoparticles as antibacterial coatings.
abstract: Ultrathin polyelectrolyte films containing silver nanoparticles appear to be a promising material for antimicrobial coatings used in the medical area. The present work is focused on the formation of multilayer polyelectrolyte films using: polyethyleneimine (PEI) as polycation, Poly(sodium 4-styrenesulfonate) (PSS) as polyanions and negatively charged silver nanoparticles (AgNPs), which led to the polyelectrolyte-silver nanocomposite coatings. The film thickness and mass were measured by ellipsometry and quartz crystal microbalance with dissipation monitoring (QCM-D) and the structure and morphology of films were visualized using scanning electron microscopy (SEM). Systematic increase of the UV-Vis absorption confirmed formation of the consecutive layers of the film. The analysis of bacteria cell adhesion to films surface was done by the luminometry measurement. Three gram-negative bacterial strains with strong adhesive properties were used in this study: Escherichia coli, Aeromonas hydrophila, and Asaia lannenesis. It was found that nanocomposite films have antimicrobial properties, which makes them very interesting for a number of practical applications, e.g. for the prevention of microbial colonization on treated surfaces.
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Krakow, Poland. Electronic address: nckruk@cyf-kr.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kruk
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Krakow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szczepanowicz
authors.affiliation: Lodz University of Technology, Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kręgiel
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Krakow, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Szyk-Warszyńska
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Krakow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Warszyński
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D053768:Metal Nanoparticles; D049329:Nanostructures; D012834:Silver / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D004573:Electrolytes; D012834:Silver
keywords: Antimicrobial coatings; Ellipsometry; Layer-by-layer technique; Polyelectrolyte multilayers; QCM-D; Silver nanoparticles adsorption