title: Characterization of canine dental pulp cells and their neuroregenerative potential.
abstract: Dental pulp cells (DPCs) of various species have been studied for their potentials of differentiation into functional neurons and secretion of neurotrophic factors. In canine, DPCs have only been studied for cell surface markers and differentiation, but there is little direct evidence for therapeutic potentials for neurological disorders. The present study aimed to further characterize canine DPCs (cDPCs), particularly focusing on their neuroregenerative potentials. It was also reported that superparamagnetic iron oxide (SPIO) particles were useful for labeling of MSCs and tracking with magnetic resonance imaging (MRI). Our data suggested that cDPCs hold higher proliferation capacity than bone marrow stromal cells, the other type of mesenchymal stem cells which have been the target of intensive research. Canine DPCs constitutively expressed neural markers, suggesting a close relationship to the nervous system in their developmental origin. Canine DPCs promoted neuritogenesis of PC12 cells, most likely through secretion of neurotrophic factors. Furthermore, SPIO nanoparticles could be effectively transported to cDPCs without significant cytotoxicity and unfavorable effects on neuritogenesis. SPIO-labeled cDPCs embedded in agarose spinal cord phantoms were successfully visualized with a magnetic resonance imaging arousing a hope for noninvasive cell tracking in transplantation studies.
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Naito
authors.affiliation: Laboratory of Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical University, Gifu, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Kudo
authors.affiliation: Laboratory of Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical University, Gifu, Japan.
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Sekine
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Kobatake
authors.affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Naritaka
authors.initials: N
authors.lastname: Tamaoki
authors.affiliation: Laboratory of Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical University, Gifu, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Inden
authors.affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Iida
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Laboratory of Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical University, Gifu, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Hozumi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan. kamicna@gifu-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival; D058948:Cell Tracking / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology; D003911:Dextrans; D004285:Dogs; D005290:Ferric Compounds; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053768:Metal Nanoparticles; D009414:Nerve Growth Factors / Q000235:genetics; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D016716:PC12 Cells; D019047:Phantoms, Imaging; D051381:Rats
chemical_list: D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D009414:Nerve Growth Factors; C000499:ferric oxide; C065507:ferumoxides
keywords: Dental pulp cells; Mesenchymal stem cells; Neurite outgrowth; Neurotrophic factors; Superparamagnetic iron oxide