title: Mesoscale blood cell sedimentation for processing millilitre sample volumes.
abstract: We demonstrate the efficient separation of blood cells from millilitre volumes of whole blood in minutes using a simple gravity sedimentation device. Blood cell and plasma separation is often the initial step in clinical diagnostics, and reliable separation techniques have remained a major obstacle for the success of point-of-care or remote diagnostics. Unlike plasma collection devices that rely solely on microchannels that restrict sample volume and throughput, we demonstrate the use of a hybrid micro/mesoscale sedimentation chamber to enable >99% capture of cells from millilitre blood samples in less than two minutes.
authors.affiliation: Electrical Technology Systems Organization, General Electric Global Research Centre. 1 Research Circle, Niskayuna, NY, 12308 USA. puleo@ge.com.
authors.forename: C
authors.initials: C
authors.lastname: Galligan
authors.forename: J
authors.initials: J
authors.lastname: Nichols
authors.forename: E
authors.initials: E
authors.lastname: Kvam
authors.forename: P
authors.initials: P
authors.lastname: Spooner
authors.forename: R
authors.initials: R
authors.lastname: Gettings
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.forename: C M
authors.initials: CM
authors.lastname: Puleo
mesh_terms: D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D019095:Point-of-Care Systems; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004247:DNA