title: Resveratrol ameliorates high glucose-induced oxidative stress injury in human umbilical vein endothelial cells by activating AMPK.
abstract: OBJECTIVE
  To investigate the effects of resveratrol on high glucose (HG)-induced vascular injury, and to establish the mechanism(s) underlying these effects.
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) were treated with glucose, and then incubated with resveratrol in the presence or absence of Compound C, an AMP-activated protein kinase (AMPK) inhibitor. Cell viability was determined using the Cell Counting Kit-8 (CCK-8) method. Reactive oxygen species, malondialdehyde, and superoxide dismutase were detected by flow cytometry, thiobarbituric acid reaction, and the nitroblue tetrazolium method, respectively. Protein levels of total and phosphorylated AMPKα and acetyl-CoA carboxylase were detected by immunoblotting.
  
  
  RESULTS
  Resveratrol significantly ameliorated HG-induced decreases in cell viability and superoxide dismutase levels and increases in reactive oxygen species and MDA levels. Moreover, resveratrol significantly reversed HG-induced dephosphorylation of AMPKα and acetyl-CoA carboxylase. However, treatment with Compound C curtailed the beneficial effects of resveratrol on HG-treated HUVECs.
  
  
  CONCLUSIONS
  Resveratrol ameliorates HG-induced injury in HUVECs by activation of AMPKα, leading to increased cellular reductive reactions and decreased oxidative stress. These results provide further evidence for resveratrol-mediated activation of AMPKα.
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Chongqing, Chongqing 400061, China.
authors.forename: Da-Yan
authors.initials: DY
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Chongqing, Chongqing 400061, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Geriatrics, Southwest Hospital of Third Military Medical University, Chongqing 400038, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Gao
authors.affiliation: Department of Geriatrics, Southwest Hospital of Third Military Medical University, Chongqing 400038, China.
authors.forename: Qi-Hong
authors.initials: QH
authors.lastname: Yang
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Chongqing, Chongqing 400061, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Chongqing, Chongqing 400061, China. Electronic address: xuqiang7147@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism*; D002478:Cells, Cultured; D003925:Diabetic Angiopathies / Q000188:drug therapy; D004353:Drug Evaluation, Preclinical; D004789:Enzyme Activation; D020536:Enzyme Activators / Q000494:pharmacology*; D005947:Glucose / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D020536:Enzyme Activators; D017382:Reactive Oxygen Species; D013267:Stilbenes; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000263:Adenylate Kinase; D005947:Glucose; D000077185:Resveratrol
keywords: AMPK; HUVEC; High glucose; Injury; Oxidative stress; Resveratrol