title: Countercurrent Separation of Natural Products: An Update.
abstract: This work assesses the current instrumentation, method development, and applications in countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC), collectively referred to as countercurrent separation (CCS). The article provides a critical review of the CCS literature from 2007 since our last review (J. Nat. Prod. 2008, 71, 1489-1508), with a special emphasis on the applications of CCS in natural products research. The current state of CCS is reviewed in regard to three continuing topics (instrumentation, solvent system development, theory) and three new topics (optimization of parameters, workflow, bioactivity applications). The goals of this review are to deliver the necessary background with references for an up-to-date perspective of CCS, to point out its potential for the natural product scientist, and thereby to induce new applications in natural product chemistry, metabolome, and drug discovery research involving organisms from terrestrial and marine sources.
authors.affiliation: §Physical Sciences Department, Rosary College of Arts and Sciences, Dominican University, River Forest, Illinois 60305, United States.
authors.forename: J Brent
authors.initials: JB
authors.lastname: Friesen
authors.forename: James B
authors.initials: JB
authors.lastname: McAlpine
authors.forename: Shao-Nong
authors.initials: SN
authors.lastname: Chen
authors.forename: Guido F
authors.initials: GF
authors.lastname: Pauli
mesh_terms: D001688:Biological Products; D003377:Countercurrent Distribution; D015394:Molecular Structure
chemical_list: D001688:Biological Products