title: Scaffolds containing chitosan/carboxymethyl cellulose/mesoporous wollastonite for bone tissue engineering.
abstract: Scaffold based bone tissue engineering utilizes a variety of biopolymers in different combinations aiming to deliver optimal properties required for bone regeneration. In the current study, we fabricated bio-composite scaffolds containing chitosan (CS), carboxymethylcellulose (CMC) with varied concentrations of mesoporous wollastonite (m-WS) particles by the freeze drying method. The CS/CMC/m-WS scaffolds were characterized by the SEM, EDS and FT-IR studies. Addition of m-WS particles had no effect on altering the porosity of the scaffolds. m-WS particles at 0.5% concentration in the CS/CMC scaffolds showed significant improvement in the bio-mineralization and protein adsorption properties. Addition of m-WS particles in the CS/CMC scaffolds significantly reduced their swelling and degradation properties. The CS/CMC/m-WS scaffolds also showed cyto-friendly nature to human osteoblastic cells. The osteogenic potential of CS/CMC/m-WS scaffolds was confirmed by calcium deposition and expression of an osteoblast specific microRNA, pre-mir-15b. Thus, the current investigations support the use of CS/CMC/m-WS scaffolds for bone tissue engineering applications.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Sainitya
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Sriram
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Kalyanaraman
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Dhivya
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Saravanan
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Vairamani
authors.affiliation: Bioproducts Laboratory, Central Leather Research Institute, Chennai, Tamil Nadu, India.
authors.forename: T P
authors.initials: TP
authors.lastname: Sastry
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India. Electronic address: selvamurugan.n@ktr.srmuniv.ac.in.
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000327:Adsorption; D018786:Bone Substitutes / Q000737:chemistry*; D002113:Calcification, Physiologic; D017610:Calcium Compounds / Q000737:chemistry*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000502:physiology; D016062:Porosity; D017640:Silicates / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes; D017610:Calcium Compounds; D017640:Silicates; D048271:Chitosan; D002266:Carboxymethylcellulose Sodium; C031293:calcium silicate
keywords: Bone; Chitosan/Carboxymethylcellulose/Mesoporous wollastonite; miR-15b