title: Acetyl Eburicoic Acid from Laetiporus sulphureus var. miniatus Suppresses Inflammation in Murine Macrophage RAW 264.7 Cells.
abstract: The basidiomycete Laetiporus sulphureus var. miniatus belongs to the Aphyllophorales, Polyporaceae, and grows on the needleleaf tree. The fruiting bodies of Laetiporus species are known to produce N-methylated tyramine derivatives, polysaccharides, and various lanostane triterpenoids. As part of our ongoing effort to discover biologically active compounds from wood-rotting fungi, an anti-inflammatory triterpene, LSM-H7, has been isolated from the fruiting body of L. sulphureus var. miniatus and identified as acetyl eburicoic acid. LSM-H7 dose-dependently inhibited the NO production in RAW 264.7 cells without any cytotoxicity at the tested concentrations. Furthermore it suppressed the production of proinflammatory cytokines, mainly inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-1β, IL-6 and tumor necrosis factor α, when compared with glyceraldehyde 3-phosphate dehydrogenase. These data suggest that LSM-H7 is a crucial component for the anti-inflammatory activity of L. sulphureus var. miniatus.
authors.affiliation: Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Saba
authors.affiliation: Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Son
authors.affiliation: Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Jeon
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: Seong-Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: In-Kyoung
authors.initials: IK
authors.lastname: Lee
authors.affiliation: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.affiliation: Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
keywords: Acetyl eburicoic acid; Anti-inflammation; Laetiporus sulphureus var. miniatus; Nitric oxide; Proinflammatory cytokine