title: Enhancement of viability of radiosensitive (PBMC) and resistant (MDA-MB-231) clones in low-dose-rate cobalt-60 radiation therapy.
abstract: OBJECTIVE
  In the present study, the authors investigated the in vitro behavior of radio-resistant breast adenocarcinoma (MDA-MB-231) cells line and radiosensitive peripheral blood mononuclear cells (PBMC), as a function of different radiation doses, dose rates and postirradiation time kinetics, with a view to the interest of clinical radiotherapy.
  
  
  METHODS
  The cells were irradiated with Co-60, at 2 and 10 Gy and two different exposure rates, 339.56 cGy.min(-1) and the other corresponding to one fourth of the standard dose rates, present over a 10-year period of cobalt therapy. Post-irradiation sampling was performed at pre-established kinetics of 24, 48 and 72 hours. The optical density response in viability assay was evaluated and a morphological analysis was performed.
  
  
  RESULTS
  Radiosensitive PBMC showed decrease in viability at 2 Gy, and a more significant decrease at 10 Gy for both dose rates. MDAMB- 231 cells presented viability decrease only at higher dose and dose rate. The results showed MDA-MB-231 clone expansion at low dose rate after 48-72 hours post-radiation.
  
  
  CONCLUSIONS
  Low dose rate shows a possible potential clinical impact involving decrease in management of radio-resistant and radiosensitive tumor cell lines in cobalt therapy for breast cancer.
authors.affiliation: Postdoc, Associate Professor at Universidade Federal do Amazonas (UFAM), Manaus, AM, Brazil.
authors.forename: Patrícia Lima
authors.initials: PL
authors.lastname: Falcão
authors.affiliation: Master, Teacher at the private education network in Belo Horizonte, MG, Brazil.
authors.forename: Bárbara Miranda
authors.initials: BM
authors.lastname: Motta
authors.affiliation: Fellow Master degree in Veterinary Medicine, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Fernanda Castro
authors.initials: FC
authors.lastname: de Lima
authors.affiliation: Master, Fellow PhD degree in Nuclear Sciences and Techniques, School of Engineering, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Celso Vieira
authors.initials: CV
authors.lastname: Lima
authors.affiliation: Postdoc, Associate Professor at Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Tarcísio Passos Ribeiro
authors.initials: TP
authors.lastname: Campos
keywords: Dose; Dose rate; LET; MDA-MB-231; PBMC; Radiation