title: Inhibition of Pancreatic Lipase and Triacylglycerol Intestinal Absorption by a Pinhão Coat (Araucaria angustifolia) Extract Rich in Condensed Tannin.
abstract: The purpose of the present work was to characterize the possible inhibition of pancreatic lipase by a tannin-rich extract obtained from the pinhão (Araucaria angustifolia seed) coat, based on the previous observation that this preparation inhibits α-amylases. Kinetic measurements of pancreatic lipase revealed that the pinhão coat tannin is an effective inhibitor. Inhibition was of the parabolic non-competitive type. The inhibition constants, Ki1 and Ki2, were equal to 332.7 ± 146.1 μg/mL and 321.2 ± 93.0 μg/mL, respectively, corresponding roughly to the inhibitor concentration producing 50% inhibition ([I]50). Consistently, the pinhão coat extract was also effective at diminishing the plasma triglyceride levels in mice after an olive oil load; 50% diminution of the area under the plasma concentration versus the time curve occurred at a dose of 250 mg/kg. This observation is most probably the consequence of an indirect inhibition of triglyceride absorption via inhibition of pancreatic lipase. For the pinhão coat tannin, this is the second report of a biological activity, the first one being a similar inhibition of the absorption of glucose derived from starch as a consequence of an inhibitory action on α-amylases. Taken together, these effects represent a potential anti-obesity action, as suggested for other polyphenol or tannin-rich preparations.
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá 87020900, Brazil. oliveiraroselene@hotmail.com.
authors.forename: Roselene Ferreira
authors.initials: RF
authors.lastname: Oliveira
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá 87020900, Brazil. gfersonag@ig.com.br.
authors.forename: Geferson Almeida
authors.initials: GA
authors.lastname: Gonçalves
authors.affiliation: Instituto Federal do Paraná, Jacarezinho 86400000, Brazil. fabiola.inacio@ifpr.edu.br.
authors.forename: Fabíola Dorneles
authors.initials: FD
authors.lastname: Inácio
authors.affiliation: Department of Nutrition, Federal University of the South Border, Realeza 85770000, Brazil. eloa-angelica@hotmail.com.
authors.forename: Eloá Angélica
authors.initials: EA
authors.lastname: Koehnlein
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá 87020900, Brazil. cgmsouza@uem.br.
authors.forename: Cristina Giatti Marques
authors.initials: CG
authors.lastname: de Souza
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá 87020900, Brazil. rmperalta@uem.com.
authors.forename: Adelar
authors.initials: A
authors.lastname: Bracht
authors.affiliation: Department of Biochemistry, State University of Maringá, Maringá 87020900, Brazil. rmperalta@uem.com.
authors.forename: Rosane Marina
authors.initials: RM
authors.lastname: Peralta
mesh_terms: D000818:Animals; D007783:Lactones / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors*; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D051379:Mice; D000069463:Olive Oil / Q000008:administration & dosage / Q000494:pharmacology; D000077403:Orlistat; D010179:Pancreas / Q000201:enzymology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D007783:Lactones; D000069463:Olive Oil; D010936:Plant Extracts; D044945:Proanthocyanidins; D014280:Triglycerides; D000077403:Orlistat; D008049:Lipase
keywords: enzyme; lipase; obesity; tannins