title: [Protective effects of recombinant human erythropoietin on oligodendrocyte after cerebral infarction].
abstract: OBJECTIVE
  To study biological effect of recombinant human erythropoietin (RhEPO) on the expression of oligodendrocyte in the neuron glia antigen 2(NG2), Nogo receptor-interacting protein 1(LINGO-1), myelin basic protein (MBP) and myelin associated glycoprotein (MAG), and to explore the protective mechanism of RhEPO for oligodendrocyte after cerebral infarction.
  
  
  METHODS
  Experimental rats were randomly divided into the treatment group (RhEPO at a dose of 3 000 U/kg) or saline control group. Both groups received intraperitoneal injection of RhEPO after cerebral ischemia in 30 min, 3 h, 6 h, 12 h and 24 h, which was administered daily for 7 days. The modified neurological severity score (mNSS) and histology were analyzed, and immunohistochemistry was used to detect the protein expression of NG2, MAG, MBP and LINGO-1.
  
  
  RESULTS
  The overall mNSS of RhEPO treatment group significantly decreased compared with the saline control group on the seventh day after cerebral infarction (P<0.05). Such treatment effect was more obvious in the treatment group at 30 min and 3 h (P<0.01). Compared with the saline control group, the numbers of NG2 positive cells increased in RhEPO treatment group. In contrast, the expression of LINGO-1 protein significantly decreased (P<0.05), with a dramatic decrease observed at 30 min and 3 h (P<0.01). However, the expression of MBP protein decreased more significantly in saline control group, while the level of the MAG protein expression increased. The differences were statistically significant (P<0.05), especially at 30 min (P<0.01).
  
  
  CONCLUSIONS
  After cerebral ischemia, RhEPO promotes the proliferation of NG2 positive cells, and inhibits the expression of LINGO-1 and MAG proteins. RhEPO improves the proliferation and differentiation of oligodendrocyte precursor cells, which in turn protects neuronal function, particularly at the early phase of ischemia.
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: He
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China; E-mail:qijiping2003@163.com.
authors.forename: Jiping
authors.initials: J
authors.lastname: Qi
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D018992:Myelin-Associated Glycoprotein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D004921:Erythropoietin; D008565:Membrane Proteins; D004676:Myelin Basic Protein; D018992:Myelin-Associated Glycoprotein; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D011994:Recombinant Proteins; D000099216:Chondroitin Sulfate Proteoglycan 4; C525164:LINGO1 protein, rat