title: Double clicking for site-specific coupling of multiple enzymes.
abstract: A method to site-specifically couple multiple enzymes is reported. The approach is based on the site-specific incorporation of a clickable non-natural amino acid into enzymes and two compatible click reactions. The multi-enzyme reaction system exhibited enhanced catalytic efficiency over the respective free enzymes.
authors.affiliation: Department of Chemical Engineering, University of Virginia, 102 Engineers' Way, Charlottesville, VA 22904, USA.
authors.forename: Sung In
authors.initials: SI
authors.lastname: Lim
authors.forename: Jinhwan
authors.initials: J
authors.lastname: Cho
authors.forename: Inchan
authors.initials: I
authors.lastname: Kwon
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D001386:Azides / Q000737:chemistry; D001681:Biological Assay; D057930:Click Chemistry; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D001386:Azides; D009097:Multienzyme Complexes