title: Augmented miR-10b expression associated with depressed expression of its target gene KLF4 involved in gastric carcinoma.
abstract: Previous studies have revealed several targets of miR-10b, such as syndecan-1, HOXD10, TBX5, and E-cadherin. In this study, we aimed to assess whether Krüppel-like factor 4 (KLF4) is a target gene of miR-10b in gastric cancer (GC). Targeting of KLF4 by miR-10b was confirmed by dual-luciferase reporter assays. The expression levels of miR-10b and KLF4 mRNA in 5 different gastric cancer cell lines and 65 pairs of gastric cancer tissues were detected by Real-time PCR. In addition, KLF4 protein in gastric cancer cell lines and 30 GC tissues was measured by western blotting and immunochemistry, respectively. KLF4 is a direct target gene of miR-10b in GC, and its expression is reduced by miR-10b at both mRNA and protein levels. In addition, the expression level of miR-10b was tendentiously upregulated in GC tissues while the expression levels of KLF4 mRNA and protein were decreased in gastric cancer tissues compared with normal adjacent tissue. There was a dramatically inverse correlation between the expression levels of miR-10b and KLF4 mRNA in GC (r=-0.339, P=0.006). These findings indicate that miR-10b was upregulated in GC and may have a key role in GC pathogenesis and development through the downregulation of its target gene KLF4.
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Yingrong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Min
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Li
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Huilian
authors.initials: H
authors.lastname: Huang
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Surgery, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Department of Pathology, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Song
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Lichen
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Surgery, Huzhou Central Hospital Huzhou, Zhejiang, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yao
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D008156:Luciferases
keywords: KLF4; gastric cancer; miR-10b; target