title: Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability.
abstract: A quantitative capillary electrophoresis (qCE) was developed by utilizing a rotary type of nano-volume injector, an autosampler, and a thermostat with cooling capacity. The accuracy and precision were greatly improved compared with conventional capillary electrophoresis. The 10 nL volume accuracy was guaranteed by the carefully designed nano-injector with an accurate internal loop. The system repeatability (precision) in terms of RSD <0.5% for migration time and 1% for peak area were achieved by using DMSO as a test sample. We believe that this fully automated qCE system has the potential to be employed broadly in quality control and quality assurance in the pharmaceutical industry.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Bang-Zan
authors.initials: BZ
authors.lastname: Ling
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.|Unimicro (Shanghai) Technologies, Pudong, Shanghai, 201203, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Zhu
authors.affiliation: Unimicro (Shanghai) Technologies, Pudong, Shanghai, 201203, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yao
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.|Unimicro (Shanghai) Technologies, Pudong, Shanghai, 201203, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D009705:Nucleosides / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D009705:Nucleosides
keywords: automation; electric decoupler; nano-valve; quantitative capillary electrophoresis