title: Ecological significance of Synergistetes in the biological treatment of tuna cooking wastewater by an anaerobic sequencing batch reactor.
abstract: Lab-scale 2L-anaerobic sequencing batch reactor was operated under mesothermic conditions. The degradation of protein-rich organic matter was determined by chemical oxygen demand, biogas production, and protein-removal activity over the operation. The structure of the microbial community was determined by qPCR and next-generation sequencing on 16S rRNA genes. At the steady state, a very efficient removal of protein (92%) was observed. Our results demonstrate a decrease of archaeal and bacterial abundance over time. Members of the phylum Synergistetes, with a peculiar emphasis for those pertaining to families Dethiosulfovibrionaceae and Aminiphilaceae, are of major ecological significance regarding the treatment of this industrial wastewater. The prominent role to be played by members of the phylum Synergistetes regarding protein and/or amino acid degradation is discussed.
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Militon
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.|Laboratoire d'écologie et biotechnologie microbienne, Institut National des Sciences Appliquées, Université de Carthage, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisie.
authors.forename: Olfa
authors.initials: O
authors.lastname: Hamdi
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Michotey
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: Laboratoire d'écologie et biotechnologie microbienne, Institut National des Sciences Appliquées, Université de Carthage, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisie.
authors.forename: Hassib
authors.initials: H
authors.lastname: Bouallagui
authors.affiliation: Laboratoire d'écologie et biotechnologie microbienne, Institut National des Sciences Appliquées, Université de Carthage, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisie.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France. patricia.bonin@mio.osupytheas.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bonin
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D003296:Cooking; D014413:Tuna; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification
chemical_list: D062065:Wastewater
keywords: Anaerobic digestion; Microbial diversity; Protein-rich effluent; Synergistetes; Tuna effluent