title: Gene Transcriptional and Metabolic Profile Changes in Mimetic Aging Mice Induced by D-Galactose.
abstract: D-galactose injection has been shown to induce many changes in mice that represent accelerated aging. This mouse model has been widely used for pharmacological studies of anti-aging agents. The underlying mechanism of D-galactose induced aging remains unclear, however, it appears to relate to glucose and 1ipid metabolic disorders. Currently, there has yet to be a study that focuses on investigating gene expression changes in D-galactose aging mice. In this study, integrated analysis of gas chromatography/mass spectrometry-based metabonomics and gene expression profiles was used to investigate the changes in transcriptional and metabolic profiles in mimetic aging mice injected with D-galactose. Our findings demonstrated that 48 mRNAs were differentially expressed between control and D-galactose mice, and 51 potential biomarkers were identified at the metabolic level. The effects of D-galactose on aging could be attributed to glucose and 1ipid metabolic disorders, oxidative damage, accumulation of advanced glycation end products (AGEs), reduction in abnormal substance elimination, cell apoptosis, and insulin resistance.
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Yue-Yue
authors.initials: YY
authors.lastname: Zhou
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Xiong-Fei
authors.initials: XF
authors.lastname: Ji
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Fu
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhu
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Li
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Chang-Kao
authors.initials: CK
authors.lastname: Mu
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Song
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D008813:Mice, Inbred ICR; D015999:Multivariate Analysis; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D005690:Galactose