title: Marine Bromophenol Derivative 3,4-Dibromo-5-(2-bromo-3,4-dihydroxy-6-isopropoxymethyl benzyl)benzene-1,2-diol Protects Hepatocytes from Lipid-Induced Cell Damage and Insulin Resistance via PTP1B Inhibition.
abstract: 3,4-Dibromo-5-(2-bromo-3,4-dihydroxy-6-isopropoxymethyl benzyl)benzene-1,2-diol (HPN) is a bromophenol derivative from the marine red alga Rhodomela confervoides. We have previously found that HPN exerted an anti-hyperglycemic property in db/db mouse model. In the present study, we found that HPN could protect HepG2 cells against palmitate (PA)-induced cell death. Data also showed that HPN inhibited cell death mainly by blocking the cell apoptosis. Further studies demonstrated that HPN (especially at 1.0 μM) significantly restored insulin-stimulated tyrosine phosphorylation of IR and IRS1/2, and inhibited the PTP1B expression level in HepG2 cells. Furthermore, the expression of Akt was activated by HPN, and glucose uptake was significantly increased in PA-treated HepG2 cells. Our results suggest that HPN could protect hepatocytes from lipid-induced cell damage and insulin resistance via PTP1B inhibition. Thus, HPN can be considered to have potential for the development of anti-diabetic agent that could protect both hepatic cell mass and function.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. luojiao2012@163.com.|The University of Chinese Academy of Sciences, Beijing 100049, China. luojiao2012@163.com.
authors.forename: Jiao
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wuning@qdio.ac.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. beckyjiang0220@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wanglijun@qdio.ac.cn.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. 12-12sy@163.com.
authors.forename: Shuaiyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. lnu101@163.com.
authors.forename: Xiangqian
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wbache@163.com.|The University of Chinese Academy of Sciences, Beijing 100049, China. wbache@163.com.
authors.forename: Baocheng
authors.initials: B
authors.lastname: Wang
authors.affiliation: Qingdao Medical University Affiliated Hospital, Qingdao 266070, China. docjack@163.com.
authors.forename: Changhui
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. shidayong@qdio.ac.cn.
authors.forename: Dayong
authors.initials: D
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001593:Benzyl Compounds / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects*; D056945:Hep G2 Cells / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007333:Insulin Resistance; D008055:Lipids / Q000009:adverse effects*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: C583476:3,4-dibromo-5-(2-bromo-3,4-dihydroxy-6-(isopropoxymethyl)benzyl)benzene-1,2-diol; D001593:Benzyl Compounds; D002396:Catechols; D007004:Hypoglycemic Agents; D008055:Lipids; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1
keywords: HPN; HepG2 cell; PTP1B inhibition; anti-cell damage; anti-insulin resistance; palmitate