title: Potent Insulin Secretagogue from Scoparia dulcis Linn of Nepalese Origin.
abstract: Ethno-botanical inspired isolation from plant Scoparia dulcis Linn. (Sweet Broomweed) yielded six compounds, coixol (1), glutinol (2), glutinone (3), friedelin (4), betulinic acid (5), and tetratriacontan-1-ol (6). There structures were identified using mass and 1D- and 2D-NMR spectroscopy techniques. Compounds 1-6 were evaluated for their insulin secretory activity on isolated mice islets and MIN-6 pancreatic β-cell line, and compounds 1 and 2 were found to be potent and mildly active, respectively. Compound 1 was further evaluated for insulin secretory activity on MIN-6 cells. Compound 1 was subjected to in vitro cytotoxicity assay against MIN-6, 3T3 cell lines, and islet cells, and in vivo acute toxicity test in mice that was found to be non-toxic. The insulin secretory activity of compounds 1 and 2 supported the ethno-botanic uses of S. dulcis as an anti-diabetic agent.
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.|Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
authors.forename: Khaga Raj
authors.initials: KR
authors.lastname: Sharma
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Achyut
authors.initials: A
authors.lastname: Adhikari
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Rahman M
authors.initials: RM
authors.lastname: Hafizur
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Hameed
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Sayed Ali
authors.initials: SA
authors.lastname: Raza
authors.affiliation: Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
authors.forename: Surya Kant
authors.initials: SK
authors.lastname: Kalauni
authors.affiliation: Division of Medicine, Graduate School of Medicine, Osaka University, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Miyazaki
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.|Department of Bio-chemistry, Faculty of Science, King Abdulaziz University, Jeddah, 21412, Saudi Arabia.
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D016475:3T3 Cells; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin; D007515:Islets of Langerhans; D008297:Male; D051379:Mice; D009390:Nepal; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D036721:Scoparia
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts
keywords: MIN-6 cells; Scoparia dulcis; coixol; insulin secretion; isolated islets