title: Involvement of flocculin in negative potential-applied ITO electrode adhesion of yeast cells.
abstract: The purpose of this study was to develop novel methods for attachment and cultivation of specifically positioned single yeast cells on a microelectrode surface with the application of a weak electrical potential. Saccharomyces cerevisiae diploid strains attached to an indium tin oxide/glass (ITO) electrode to which a negative potential between -0.2 and -0.4 V vs. Ag/AgCl was applied, while they did not adhere to a gallium-doped zinc oxide/glass electrode surface. The yeast cells attached to the negative potential-applied ITO electrodes showed normal cell proliferation. We found that the flocculin FLO10 gene-disrupted diploid BY4743 mutant strain (flo10Δ /flo10Δ) almost completely lost the ability to adhere to the negative potential-applied ITO electrode. Our results indicate that the mechanisms of diploid BY4743 S. cerevisiae adhesion involve interaction between the negative potential-applied ITO electrode and the Flo10 protein on the cell wall surface. A combination of micropatterning techniques of living single yeast cell on the ITO electrode and omics technologies holds potential of novel, highly parallelized, microchip-based single-cell analysis that will contribute to new screening concepts and applications.
authors.affiliation: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan skoyama@jamstec.go.jp.
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Koyama
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Tsubouchi
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Usui
authors.affiliation: Department of Marine Science, Marine Works Japan Ltd., 3-54-1 Oppamahigashi, Yokosuka 237-0063, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Uematsu
authors.affiliation: Department of Marine Science, Marine Works Japan Ltd., 3-54-1 Oppamahigashi, Yokosuka 237-0063, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tame
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nogi
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Ohta
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatada
authors.affiliation: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
authors.affiliation: Marine Technology and Engineering Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Miwa
authors.affiliation: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Toyofuku
authors.affiliation: Department of Foods and Human Nutrition, Notre Dame Seishin University, 2-16-9 Ifuku-cho, Kita-ku, Okayama 700-8516, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Nagahama
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Konishi
authors.affiliation: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Nagano
authors.affiliation: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan.
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Abe
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D004566:Electrodes / Q000382:microbiology*; D017353:Gene Deletion; D005898:Glass; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017971:Tin Compounds; D015034:Zinc Oxide
chemical_list: D015815:Cell Adhesion Molecules; C000606935:Flo10 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017971:Tin Compounds; C109984:indium tin oxide; D015034:Zinc Oxide
keywords: Flo10; Saccharomyces cerevisiae; electrical attachment; indium tin oxide; potential-controlled electrode; single-cell cultivation