title: Mmu-miR-126a-3p plays a role in murine embryo implantation by regulating Itga11.
abstract: miR-126a-3p has been found to be specifically up-regulated in the process of murine embryo implantation. This study aimed to further clarify the role of miR-126a-3p in embryo implantation. The expression of miR-126a-3p in implantation sites was significantly higher than that in interimplantation sites (P = 0.009). Its expression dynamics in a series of models, including pseudopregnancy, delayed implantation and artificial decidualization, suggested that the induction of miR-126a-3p is dependent on hormonal signalling and decidualization of the endometrium. Bioinformatic analysis predicted and luciferase activity assay confirmed that Itga11, a member of the integrin family, was a target gene of miR-126a-3p. miR-126a-3p bound to the 3' untranslated region of Itga11 and regulated Itga11 by inhibiting mRNA translation and affecting mRNA stability. Transwell assay showed that miR-126a-3p promoted cell migratory and invasive capacity in vitro. Loss of function of miR-126a-3p significantly reduced the number of implantation sites in vivo (P = 0.013). Collectively, miR-126a-3p may play a major role in embryo implantation by regulating Itga11, possibly through impairing cell migratory and invasive capacity. These findings should contribute to a better understanding of the miRNA-based mechanism of embryo implantation.
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 610041 Chengdu, China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, 610041 Chengdu, China. Electronic address: zhengyuli@scu.edu.cn.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 610041 Chengdu, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 610041 Chengdu, China.
authors.forename: Jinhai
authors.initials: J
authors.lastname: Gou
authors.affiliation: State Key Laboratory of Biotherapy, West China University Hospital, Sichuan University, 610041 Chengdu, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Biotherapy, West China University Hospital, Sichuan University, 610041 Chengdu, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 610041 Chengdu, China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, 610041 Chengdu, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, 610041 Chengdu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D039001:Integrin alpha Chains / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: D039001:Integrin alpha Chains; C545201:MIRN126 microRNA, mouse; D035683:MicroRNAs
keywords: Itga11; embryo implantation; integrin; miR-126a-3p; microRNA