title: [Effects of hydrogen-rich saline on Akt/GSK3β signaling pathways and cardiac function during myocardial ischemia-reperfusion in rats].
abstract: OBJECTIVE
  To explore the effects of hydrogen-rich saline on Akt/GSK3β signaling pathways and cardiac function during myocardial ischemia-reperfusion (I/R) in rats.
  
  
  METHODS
  A total of 150 healthy male Sprague-Dawley rats were selected and divided randomly into 5 groups of normal control (I), sham operation (II), myocardial ischemia-reperfusion (III), hydrogen-rich saline (IV) and normal saline group (V) (n = 30 each). Group I had no treatment at all. In group II, anterior descending branch was merely exposed but not ligated. Myocardial I/R was induced by 30-min occlusion of anterior descending branch of left coronary artery followed by 12/24 h reperfusion of Bimbaum. Hydrogen-rich saline 1 ml/100 g were injected intraperitoneally 5 min before reperfusion in group IV. Normal saline 1 ml/100 g was injected intraperitoneally 5 min before reperfusion in group V. The values of ± dp/dt max, left ventricular diastolic pressure (LVDP) and left ventricular systolic pressure (LVSP) during the procedure were recorded by BL-420 biological function experimental system at pre-ischemia, ischemia 30 min, 60 min and 120 min of reperfusion. The animals were sacrificed at 60 min, 120 min of reperfusion and hearts harvested. The pathological changes of myocardial tissue were detected by hematoxylin and eosin staining. And the expressions of Akt and GSK3β in myocardial tissue were examined by Western blot.
  
  
  RESULTS
  Compared with groups I and II, the values of ± dp/dt max, LVDP and LVSP significantly increased. And the expressions of Akt and GSK3β in myocardial tissue significantly increased in groups III, IV and V. Compared with groups III and V, the values of ± dp/dt max, LVDP and LVSP significantly decreased. And the expressions of Akt and GSK3β in myocardial tissue significantly decreased in group IV. Compared with T0, the values of ± dp/dt max, LVDP and LVSP significantly decreased at T(1-3) in groups III, IV and V. Compared with T(1), the values of ± dp/dt max, LVDP and LVSP significantly increased at T(2-3) in group IV. Compared with T(2), the expressions of Akt and GSK3β in myocardial tissue significantly decreased at T(3) in group IV.
  
  
  CONCLUSIONS
  Hydrogen-rich saline can reduce myocardial reperfusion injury and improve heart function. The mechanism may be due to associated with the down-regulated expressions of Akt and GSK3β in myocardial tissue.
authors.affiliation: Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163411, China.
authors.forename: Le
authors.initials: L
authors.lastname: Yue
authors.affiliation: Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163411, China; Email: 54866880@qq.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163411, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163411, China.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiology, Daqing Oil Field General Hospital, Daqing 163411, China.
authors.forename: Boying
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D002320:Cardiovascular Physiological Phenomena; D003324:Coronary Artery Disease; D038362:Glycogen Synthase Kinase 3; D000071679:Glycogen Synthase Kinase 3 beta; D006859:Hydrogen; D008297:Male; D017202:Myocardial Ischemia; D015428:Myocardial Reperfusion Injury; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride; D006859:Hydrogen; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3