title: Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.
abstract: The roles of non-cellulosic polysaccharides in cotton fiber development are poorly understood. Combining glycan microarrays and in situ analyses with monoclonal antibodies, polysaccharide linkage analyses and transcript profiling, the occurrence of heteromannan and heteroxylan polysaccharides and related genes in developing and mature cotton (Gossypium spp.) fibers has been determined. Comparative analyses on cotton fibers at selected days post-anthesis indicate different temporal and spatial regulation of heteromannan and heteroxylan during fiber development. The LM21 heteromannan epitope was more abundant during the fiber elongation phase and localized mainly in the primary cell wall. In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber. These developmental dynamics were supported by transcript profiling of biosynthetic genes. Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls. In addition, the relative abundance of these epitopes during fiber development varied between cotton lines with contrasting fiber characteristics from four species (G. hirsutum, G. barbadense, G. arboreum and G. herbaceum), suggesting that these non-cellulosic polysaccharides may be involved in determining final fiber quality and suitability for industrial processing.
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.
authors.forename: Mercedes C
authors.initials: MC
authors.lastname: Hernandez-Gomez
authors.affiliation: Bayer CropScience NV-Innovation Center, Technologiepark 38, 9052 Gent, Belgium These authors contributed equally to this work.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Runavot
authors.affiliation: Department of Plant and Environmental Sciences, University of CopenhagenThorvaldsensvej 40, 1871 Frederiksberg C, Copenhagen, Denmark.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Bayer CropScience NV-Innovation Center, Technologiepark 38, 9052 Gent, Belgium.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bourot
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Thomas A S
authors.initials: TA
authors.lastname: Benians
authors.affiliation: Department of Plant and Environmental Sciences, University of CopenhagenThorvaldsensvej 40, 1871 Frederiksberg C, Copenhagen, Denmark.
authors.forename: William G T
authors.initials: WG
authors.lastname: Willats
authors.affiliation: Bayer CropScience NV-Innovation Center, Technologiepark 38, 9052 Gent, Belgium.
authors.forename: Frank
authors.initials: F
authors.lastname: Meulewaeter
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK j.p.knox@leeds.ac.uk.
authors.forename: J Paul
authors.initials: JP
authors.lastname: Knox
mesh_terms: D001696:Biomechanical Phenomena; D002473:Cell Wall / Q000378:metabolism*; D016000:Cluster Analysis; D032561:Cotton Fiber; D000939:Epitopes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008351:Mannans / Q000378:metabolism*; D046228:Microarray Analysis; D009005:Monosaccharides / Q000032:analysis; D013045:Species Specificity; D014990:Xylans / Q000378:metabolism*
chemical_list: D000939:Epitopes; D008351:Mannans; D009005:Monosaccharides; D014990:Xylans
keywords: Cotton; Fiber cell development; Gossypium spp; Heteromannan; Heteroxylan; Plant cell walls