title: -308 G/A TNF-α gene polymorphism influences the course of basal cell carcinoma in a Polish population.
abstract: BACKGROUND
  The etiopathogenesis of basal cell carcinoma (BCC) is multifactorial. The TNF-α gene seems to be an interesting gene candidate for BCC susceptibility because of the proinflammatory and immunosuppressive properties of its product. The aim of the study was to assess the frequency of -308 G/A and -238 G/A gene polymorphisms in the TNF-α gene and serum levels of cytokine in patients with BCC.
  
  
  METHODS
  The study included 176 (94 women, 82 men) patients with BCC and 261 healthy volunteers. -308 G/A and -238 G/A TNF-α polymorphisms were analyzed using the amplification refractory mutation system-polymerase chain reaction method (ARMS-PCR). Serum concentrations of TNF-α were measured using ELISA.
  
  
  RESULTS
  There was no statistically significant association between allele, genotype and haplotype frequencies in BCC patients in comparison with controls. Occurrence of the -308 TNF-α A allele or GA genotype in the group of patients with BCC increases risk of recurrence of tumor recurrence (OR = 4.8, 95% CI: 1.6-13.9, p = 0.004 and OR = 4.97, 95% CI: 1.7-14.5, p = 0.004). Moreover, -308 TNF-α GG genotype decreased risk of recurrence (OR = 0.2, 95% CI: 0.07-0.6, p = 0.004). The -238/-308 GA haplotype was connected with increased risk of recurrence (OR = 4.36, 95% CI: 1.49-12.7, p = 0.007). We also found significantly higher TNF-α levels among BCC patients in comparison with controls (p = 0.004).
  
  
  CONCLUSIONS
  The obtained results did not confirm the role of the -308 G/A and -238 G/A TNF-α gene polymorphisms in BCC development, but the presence of the A allele or GA genotype in -308 G/A TNF-α gene polymorphism may have an impact on the course of the disease.
authors.affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Sobjanek
authors.affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Zabłotna
authors.affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Igor
authors.initials: I
authors.lastname: Michajłowski
authors.affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Nedoszytko
authors.affiliation: Department of Dermatology and Venerology, Medical University of Lodz, Lodz, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Lesiak
authors.affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Nowicki
keywords: TNF-α; basal cell carcinoma; gene polymorphism; tumor necrosis factor α