title: Fast and Accurate Large-Scale Detection of β-Lactamase Genes Conferring Antibiotic Resistance.
abstract: Fast detection of β-lactamase (bla) genes allows improved surveillance studies and infection control measures, which can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited bla gene types, these methods have significant limitations, such as their failure to detect almost all clinically available bla genes. We developed a fast and accurate molecular method to overcome these limitations using 62 primer pairs, which were designed through elaborate optimization processes. To verify the ability of this large-scale bla detection method (large-scaleblaFinder), assays were performed on previously reported bacterial control isolates/strains. To confirm the applicability of the large-scaleblaFinder, the assays were performed on unreported clinical isolates. With perfect specificity and sensitivity in 189 control isolates/strains and 403 clinical isolates, the large-scaleblaFinder detected almost all clinically available bla genes. Notably, the large-scaleblaFinder detected 24 additional unreported bla genes in the isolates/strains that were previously studied, suggesting that previous methods detecting only limited types of bla genes can miss unexpected bla genes existing in pathogenic bacteria, and our method has the ability to detect almost all bla genes existing in a clinical isolate. The ability of large-scaleblaFinder to detect bla genes on a large scale enables prompt application to the detection of almost all bla genes present in bacterial pathogens. The widespread use of the large-scaleblaFinder in the future will provide an important aid for monitoring the emergence and dissemination of bla genes and minimizing the spread of resistant bacteria.
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Jung Hun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Dae Beom
authors.initials: DB
authors.lastname: Kwon
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Jeon
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Kwang Seung
authors.initials: KS
authors.lastname: Park
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Chang-Ro
authors.initials: CR
authors.lastname: Lee
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea sangheelee@mju.ac.kr.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000737:chemistry* / Q000378:metabolism; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D017931:DNA Primers; D047090:beta-Lactams; D001618:beta-Lactamases