title: Adaptive Evolution of CENP-A in Percid Fishes.
abstract: Centromeric protein A (CENP-A) is the epigenetic determinant of centromeres. This protein has been shown to be adaptively evolving in a number of animal and plant species. In a previous communication we were able to demonstrate that signs of adaptive evolution were detected in the comparison of CENP-A sequences from three percid fish species. In this study we isolated the CENP-A gene from eight additional species from the Percidae family. With these sequences and those previously obtained, we carried out a more robust statistical analysis of codon specific positive selection in CENP-A coding sequences of eleven percid species. We were able to demonstrate that at least two amino acid positions within the N-terminal tail are under strong positive selection and that one of these positions is potentially a substrate for phosphorylation. While nonsynonymous substitutions were detected in the histone fold domain, these were not statistically supported as resulting from positive selection.
authors.affiliation: Department of Biology, University of West Georgia, Carrollton, GA 30118, USA. hallietus@gmail.com.
authors.forename: Harriet N A
authors.initials: HN
authors.lastname: Abbey
authors.affiliation: Department of Biology, University of West Georgia, Carrollton, GA 30118, USA. lkral@westga.edu.
authors.forename: Leos G
authors.initials: LG
authors.lastname: Kral
keywords: CENP-A; adaptive evolution; fishes; percids