title: Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts.
abstract: Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.
authors.affiliation: Institute for Research in Biomedicine (IRB-Barcelona), Barcelona, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Pampalona
authors.affiliation: Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee, UK.
authors.forename: Jens
authors.initials: J
authors.lastname: Januschke
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
authors.affiliation: Institute for Research in Biomedicine (IRB-Barcelona), Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain.
authors.forename: Cayetano
authors.initials: C
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018385:Centrosome / Q000502:physiology / Q000648:ultrastructure*; D004330:Drosophila; D007814:Larva / Q000166:cytology; D008856:Microscopy, Fluorescence; D058953:Neural Stem Cells / Q000648:ultrastructure*; D061251:Primary Cell Culture; D059008:Time-Lapse Imaging
keywords: Cell culture; Centriole; Centrosome; Drosophila; Microscopy; Neuroblast