title: Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil.
abstract: Bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. Although the infection has been described in cattle in some countries, including in Brazil, there are only two reports of infection in buffaloes: one in Pakistan and one in Cambodia. The aim of the present study was to survey the occurrence of bovine immunodeficiency virus (BIV) in water buffaloes from the Amazon region, Pará state, Brazil. BIV proviral DNA was surveyed in 607 whole blood samples of water buffaloes from 10 farms located in the state of Pará using semi-nested polymerase chain reaction (PCR) (PCR-SN) to amplify the pol region of the viral genome. Of the 607 samples tested, 27 (4.4 %) were positive for BIV proviral DNA. The amplified fragments were confirmed by sequence analysis after cloning and nucleotide sequencing. The sequence obtained had 99 % similarity to the reference strain (R-29). The present study provides important epidemiological data because BIV was detected for the first time in water buffaloes in Brazil. Further, the results suggest the possibility of the virus being a risk factor for herd health because it may be a potential causal agent of chronic disease and, also may be associated to other infectious diseases.
authors.affiliation: Instituto de Medicina Veterinária, Hospital Veterinário de Grandes Animais, Universidade Federal do Pará, Rodovia BR 316 Km 61, Bairro Saudade, 68740-970, Castanhal, PA, Brazil. tatyalbernaz@ufpa.br.
authors.forename: Tatiane Teles
authors.identifier: http://orcid.org/0000-0002-1538-6140
authors.initials: TT
authors.lastname: Albernaz
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Rômulo Cerqueira
authors.initials: RC
authors.lastname: Leite
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Jenner Karlison Pimenta
authors.initials: JK
authors.lastname: Reis
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Sousa Rodrigues
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Telissa
authors.initials: T
authors.lastname: da Cunha Kassar
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Claudia Fideles
authors.initials: CF
authors.lastname: Resende
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Cairo Henrique Sousa
authors.initials: CH
authors.lastname: de Oliveira
authors.affiliation: Instituto de Medicina Veterinária, Hospital Veterinário de Grandes Animais, Universidade Federal do Pará, Rodovia BR 316 Km 61, Bairro Saudade, 68740-970, Castanhal, PA, Brazil.
authors.forename: Rafaela das Mercês
authors.initials: Rd
authors.lastname: Silva
authors.affiliation: Instituto de Biodiversidade e Florestas, Universidade Federal do Oeste do Pará, Santarém, PA, Brazil.
authors.forename: Felipe Masiero
authors.initials: FM
authors.lastname: Salvarani
authors.affiliation: Instituto de Medicina Veterinária, Hospital Veterinário de Grandes Animais, Universidade Federal do Pará, Rodovia BR 316 Km 61, Bairro Saudade, 68740-970, Castanhal, PA, Brazil.
authors.forename: José Diomedes
authors.initials: JD
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002020:Buffaloes / Q000821:virology*; D002160:Cambodia; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification; D016679:Genome, Viral; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary*; D007962:Leukocytes / Q000166:cytology; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012307:Risk Factors
chemical_list: D004279:DNA, Viral
keywords: BIV; Bovine immunodeficiency virus; Brazil; Buffalo; PCR