title: Investigation of Protein-Protein Interactions and Conformational Changes in Hedgehog Signaling Pathway by FRET.
abstract: Protein-protein interactions and signal-induced protein conformational changes are fundamental molecular events that are considered as essential in modern life sciences. Among various techniques developed to study such phenomena, fluorescence resonance energy transfer (FRET) is a widely used method with many advantages in detecting these molecular events. Here, we describe the application of FRET in the mechanistic investigation of cell signal transduction, taking the example of the Hh signaling pathway, which plays a critical role in embryonic development and tissue homeostasis. A number of general guidelines as well as some key notes have been summarized as a protocol for reader's reference.
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Fu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Lv
authors.forename: Yue
authors.initials: Y
authors.lastname: Xiong
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002460:Cell Line; D004330:Drosophila; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D015870:Gene Expression; D053823:Hedgehog Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D015398:Signal Transduction; D014921:Wings, Animal / Q000378:metabolism
chemical_list: D053823:Hedgehog Proteins