title: A Rodent Model to Evaluate the Tissue Response to a Biological Scaffold When Adjacent to a Synthetic Material.
abstract: The use of biologic scaffold materials adjacent to synthetic meshes is commonplace. A prevalent clinical example is two-staged breast reconstruction, where biologic scaffolds are used to provide support and coverage for the inferior aspect of the synthetic expander. However, limited data exist regarding either the kinetics of biologic scaffold integration or the host tissue response to the biologic scaffold materials used for this application or other applications in which such scaffold materials are used. The present study evaluated the temporal host response to a biological scaffold when placed adjacent to a synthetic material. Evaluation criteria included quantification of material contracture and characterization of the host cell response and tissue remodeling events. Results show a decreased thickness of the collagenous tissue layer at biologic scaffold/silicone interface compared to the abdominal wall/silicone interface during the 12-week experimental time course. All test materials were readily incorporated into surrounding host tissue.
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.|3 DoD-VA Extremity Trauma and Amputation Center of Excellence, Walter Reed National Military Medical Center , Bethesda, Maryland.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Dearth
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|4 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Keane
authors.affiliation: 5 Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University , Providence, Rhode Island.|6 C.R. Bard, Inc. (Davol) , Providence, Rhode Island.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Scott
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Daly
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.|4 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Badylak
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D003238:Connective Tissue / Q000187:drug effects; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials