title: 3,6-Dimethoxy-6″,6″-Dimethyl-(7,8,2″,3″)-Chromeneflavone, a Flavonoid Isolated from Lonchocarpus Araripensis Benth. (Fabaceae), Reduces Nociceptive Behaviour in Mice.
abstract: Lonchocarpus araripensis Benth. is largely distributed in the northeast region of Brazil. It is popularly known as 'sucupira'. Recent studies have shown that some species of Lonchocarpus have interesting pharmacological activities. In this study, we evaluated the antinociceptive effect of a flavone isolated from L. araripensis. The chemical examination resulted in the isolation of 3,6-dimethoxy-6″,6″-dimethyl-(7,8,2″,3″)-chromeneflavone (DDF). The structure of the compound was established by spectral analysis. Antinociceptive activity of DDF was evaluated by measuring nociception by acetic acid, formalin and hot plate tests. The rota rod test was used to evaluate motor coordination. The results demonstrated that DDF was able to prevent acetic-acid-writhing-induced nociception (p < 0.001) in mice. Furthermore, DDF produced a significant reduction of the nociceptive behaviour at the early and late phases of paw licking in the formalin test. Also, DDF produced an inhibition of the nociceptive behaviour during a hot-plate test. No alteration in motor coordination was observed. These results confirm the hypothesis that DDF reduces the nociceptive behaviour in mice, probably through central mechanisms, but without compromising the motor coordination of animals.
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Jackson R G S
authors.initials: JR
authors.lastname: Almeida
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Juliane C
authors.initials: JC
authors.lastname: Silva
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Oliveira
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Grasielly R
authors.initials: GR
authors.lastname: Souza
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Raimundo G
authors.initials: RG
authors.lastname: Oliveira-Júnior
authors.affiliation: Centre for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
authors.forename: Sarah R G
authors.initials: SR
authors.lastname: Lima-Saraiva
authors.affiliation: Departament of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Barbosa-Filho
authors.affiliation: Departament of Chemistry of Natural Products, State University of North Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors.forename: Raimundo
authors.initials: R
authors.lastname: Braz-Filho
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Dinalva Brito
authors.initials: DB
authors.lastname: Queiroz
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Marco Antônio
authors.initials: MA
authors.lastname: Botelho
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001938:Brazil; D007887:Fabaceae / Q000737:chemistry; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D051379:Mice; D059225:Nociception / Q000187:drug effects*; D010147:Pain Measurement; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D000700:Analgesics; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts; D019342:Acetic Acid; C043562:flavone
keywords: Brazilian plants; Fabaceae; Lonchocarpus araripensis; flavone; flavonoid; pain