title: The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction.
abstract: Laccases, blue copper-containing oxidases, ≿ an play an important role in a variety of natural processes. The majority of fungal laccases are encoded by multigene families that express closely related proteins with distinct functions. Currently, only the properties of major gene products of the fungal laccase families have been described. Our study is focused on identification and characterization of laccase genes, which are transcribed in basidiomycete Trametes hirsuta 072, an efficient lignin degrader, in a liquid medium, both without and with induction of laccase transcription by copper ions. We carried out production of cDNA libraries from total fungal RNA, followed by suppression subtractive hybridization and mirror orientation selection procedures, and then used Next Generation Sequencing to identify low abundance and differentially expressed laccase transcripts. This approach resulted in description of five laccase genes of the fungal family, which, according to the phylogenetic analysis, belong to distinct clusters within the Trametes genus. Further analysis established similarity of physical, chemical, and catalytic properties between laccases inside each cluster. Structural modeling suggested importance of the sequence differences in the clusters for laccase substrate specificity and catalytic efficiency. The implications of the laccase variations for the fungal physiology are discussed.
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia. Electronic address: d.v.vasina@gmail.com.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Vasina
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, st. Botanicheskaya, 35, Moscow 127276, Russia.
authors.forename: Orkhan N
authors.initials: ON
authors.lastname: Mustafaev
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Moiseenko
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, st. Botanicheskaya, 35, Moscow 127276, Russia.
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Sadovskaya
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Glazunova
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, st. Botanicheskaya, 35, Moscow 127276, Russia.
authors.forename: Аlexander А
authors.initials: АА
authors.lastname: Tyurin
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Fedorova
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia.
authors.forename: Andrey R
authors.initials: AR
authors.lastname: Pavlov
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Tyazhelova
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, st. Botanicheskaya, 35, Moscow 127276, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Goldenkova-Pavlova
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospekt, 33, build. 2, Moscow 119071, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Koroleva
mesh_terms: D003300:Copper / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D042845:Laccase / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D055454:Trametes / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D003300:Copper; D042845:Laccase
keywords: Differential expression; Laccase multigene family; Phylogenetic analysis; White-rot fungi