title: Aloe vera Extract Suppresses Proliferation of Neuroblastoma Cells In Vitro.
abstract: OBJECTIVE
  Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract.
  
  
  METHODS
  Human neuroblastoma cell lines (IMR-32, TGW, CHP-126 and NBL-S) were cultured with AV protein extract and proliferation status was assessed by cell counting, Ki-67 staining and gene expression.
  
  
  RESULTS
  Among tested lines, the number of viable, AV-treated IMR-32 cells significantly decreased 1.98-fold by day 2 and 1.33-fold by day 5 of culture relative to untreated controls (p<0.05). Treatment also decreased the number of Ki-67(+) IMR-32 cells by 13% by day 5 (p<0.05) and, unlike untreated controls, CCND2 mRNA expression levels became undetectable by day 1.
  
  
  CONCLUSIONS
  AV-protein extract suppresses human IMR-32 neuroblastoma cell proliferation, possibly by suppressing CCND2 transcript levels in vitro.
authors.affiliation: Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Yonehara
authors.affiliation: Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kasem
authors.initials: K
authors.lastname: Kulkeaw
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Era
authors.affiliation: Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
authors.affiliation: Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan ds-mons@yb3.so-net.ne.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sugiyama
mesh_terms: D000504:Aloe / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D056742:Cyclin D2 / Q000235:genetics; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000970:Antineoplastic Agents; C080336:CCND2 protein, human; D056742:Cyclin D2; D010936:Plant Extracts; D012333:RNA, Messenger
keywords: Aloe vera; Neuroblastoma; proliferation