title: Lutein alleviates arsenic-induced reproductive toxicity in male mice via Nrf2 signaling.
abstract: This study aims to investigate the mechanisms involved in the action of lutein (LU) alleviating arsenic-induced reproductive toxicity using mice model. Forty male Kunming mice were received following treatments by gavage: normal saline solution (control), arsenic trioxide (ATO; 5 mg/kg/day), LU (40 mg/kg/day), and ATO + LU (5 mg/kg/day + 40 mg/kg/day). At the end, the mice were killed by cervical dislocation and weighed. Pathological examination was done on the testis. The biomedical parameters including superoxide dismutase (SOD), glutathione (GSH), total antioxidative capability, malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and reproductive indexes were analyzed. The messenger RNA (mRNA) and protein expression of Nrf2, heme oxygenase 1 (HO-1), glutathione S-transferase (GST), nicotinamide adenine dinucleotide phosphate dehydrogenase, quinone 1 (NQO1) in testis were detected by real-time polymerase chain reaction and Western blot. We found that there was a decrease in sperm count; testis somatic index; the activities of SOD, GSH, total antioxidative capacity (p < 0.01, respectively) in ATO-treated mice, while there was an increase in the levels of sperm abnormalities, MDA, and 8-OHdG than control (p < 0.01, respectively). The groups treated with ATO + LU showed recovery of the measured parameters between those of ATO or saline-treated group. The antagonized interaction between ATO and LU was statistically significant (p < 0.01). Mice treated with ATO + LU also showed greater mRNA expression of Nrf2, HO-1, NQO1, and GST than ATO or saline-treated groups. These findings suggest that LU alleviates reproductive toxicity induced by arsenic in male mice via Nrf2 signaling, which implicates a possible mechanism of LU in preventing the reproductive injury, and elucidates that consuming the rich plant sources of LU will alleviate the reproductive toxicity induced by chemicals.
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: S G
authors.initials: SG
authors.lastname: Li
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Xu
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Niu
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Ding
authors.affiliation: Department of Pathology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Pang
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: R L
authors.initials: RL
authors.lastname: Ma
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: M X
authors.initials: MX
authors.lastname: Jing
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Pathology, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Ma
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Feng
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Liu
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Xiang
authors.affiliation: Department of Pathology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), School of Medicine, Shihezi University, Shihezi, Xinjiang, China lifeng78551@126.com.
authors.forename: F
authors.initials: F
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals; D001835:Body Weight / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D014975:Lutein / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008815:Mice, Inbred Strains; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D001152:Arsenicals; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D010087:Oxides; D014874:Water Pollutants, Chemical; D000077237:Arsenic Trioxide; D014975:Lutein
keywords: Lutein; Nrf2 signaling; arsenic; chemotherapy; mouse model; reproductive toxicology