title: Rational design of digital assays.
abstract: Optimum algorithm for digital assays treats chemical compartments as bits of probabilistic information and arranges these bits in a fractional positional system. Maximization of information gain reduces, by orders of magnitude, the number of partitions required to achieve the requested dynamic range and precision of the assay. The method simplifies the execution of digital analytical methods providing for more accessible use of absolute quantization in research and in diagnostics.
authors.affiliation: †Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.|‡Curiosity Diagnostics Sp. z o.o. Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Pawel R
authors.initials: PR
authors.lastname: Debski
authors.affiliation: ‡Curiosity Diagnostics Sp. z o.o. Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Gewartowski
authors.affiliation: ‡Curiosity Diagnostics Sp. z o.o. Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sulima
authors.affiliation: †Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.|‡Curiosity Diagnostics Sp. z o.o. Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Tomasz S
authors.initials: TS
authors.lastname: Kaminski
authors.affiliation: †Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.|‡Curiosity Diagnostics Sp. z o.o. Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Garstecki