title: Identification and characterization of a late gene of grouper iridovirus 61l and antibody production against the protein encoded by it.
abstract: In this study, a late gene encoded by grouper iridovirus, giv-61L, was identified and classified, and mouse monoclonal antibodies (mAbs) were raised against this protein. Giv-61L homologues were found only in the genus Ranavirus. Three mAbs to Giv-61L protein were produced. In drug inhibition assays, giv-61L was identified as a late gene. Finally, GIV-61L-mAb-8 was used in western blotting and immunofluorescence assays to demonstrate that Giv-61L protein was included in the GIV particle, expressed at 18 h, and localized only in the cytoplasm of GIV-infected cells. The results of this study provide insight into GIV pathogenesis and GIV-61L-mAbs will have broad applications in GIV immunodiagnostics.
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University 1, Sec. 1, Shen-Lung Road, Yilan, 26047, Taiwan.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University 1, Sec. 1, Shen-Lung Road, Yilan, 26047, Taiwan.
authors.forename: W Y
authors.initials: WY
authors.lastname: Hsieh
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University 1, Sec. 1, Shen-Lung Road, Yilan, 26047, Taiwan.
authors.forename: Y S
authors.initials: YS
authors.lastname: Lai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003593:Cytoplasm; D005260:Female; D006825:Hybridomas; D017994:Iridovirus / Q000235:genetics*; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010473:Perciformes / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D014764:Viral Proteins
keywords: Giv-61L; grouper iridovirus; immunofluorescence; mAbs