title: Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile.
abstract: Mesenchymal stromal cells (MSCs) are cultured cells that can give rise to mature mesenchymal cells under appropriate conditions and secrete a number of biologically relevant molecules that may play an important role in regenerative medicine. Evidence indicates that pericytes (PCs) correspond to mesenchymal stem cells in vivo and can give rise to MSCs when cultured, but a comparison between the gene expression profiles of cultured PCs (cPCs) and MSCs is lacking. We have devised a novel methodology to isolate PCs from human adipose tissue and compared cPCs to MSCs obtained through traditional methods. Freshly isolated PCs expressed CD34, CD140b, and CD271 on their surface, but not CD146. Both MSCs and cPCs were able to differentiate along mesenchymal pathways in vitro, displayed an essentially identical surface immunophenotype, and exhibited the ability to suppress CD3(+) lymphocyte proliferation in vitro. Microarray expression data of cPCs and MSCs formed a single cluster among other cell types. Further analyses showed that the gene expression profiles of cPCs and MSCs are extremely similar, although MSCs differentially expressed endothelial cell (EC)-specific transcripts. These results confirm, using the power of transcriptomic analysis, that PCs give rise to MSCs and suggest that low levels of ECs may persist in MSC cultures established using traditional protocols.
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .|2 Laboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Lutheran University of Brazil , Canoas, Brazil .
authors.forename: Lindolfo
authors.initials: L
authors.lastname: da Silva Meirelles
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Tathiane Maistro
authors.initials: TM
authors.lastname: Malta
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Virgínia Mara
authors.initials: VM
authors.lastname: de Deus Wagatsuma
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Patrícia Viana Bonini
authors.initials: PV
authors.lastname: Palma
authors.affiliation: 3 Laboratory of Large-Scale Functional Biology (LLSFBio), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Amélia Goes
authors.initials: AG
authors.lastname: Araújo
authors.affiliation: 4 School of Pharmaceutical Sciences of Ribeirão Preto, Universidade de São Paulo , Ribeirão Preto, Brazil .
authors.forename: Kelen Cristina
authors.initials: KC
authors.lastname: Ribeiro Malmegrim
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Fábio
authors.initials: F
authors.lastname: Morato de Oliveira
authors.affiliation: 3 Laboratory of Large-Scale Functional Biology (LLSFBio), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Rodrigo Alexandre
authors.initials: RA
authors.lastname: Panepucci
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .|5 Department of Genetics, School of Medicine of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Wilson Araújo
authors.initials: WA
authors.lastname: Silva
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Simone
authors.initials: S
authors.lastname: Kashima Haddad
authors.affiliation: 1 Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .|6 Department of Clinical Medicine, School of Medicine of Ribeirão Preto, University of São Paulo , Ribeirão Preto, Brazil .
authors.forename: Dimas Tadeu
authors.initials: DT
authors.lastname: Covas
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D020286:Pericytes / Q000166:cytology / Q000378:metabolism*; D061251:Primary Cell Culture / Q000379:methods; D059467:Transcriptome
chemical_list: D015703:Antigens, CD