title: miR-203 suppresses the proliferation and metastasis of hepatocellular carcinoma by targeting oncogene ADAM9 and oncogenic long non-coding RNA HULC.
abstract: MicroRNAs (miRNAs) have been integrated into tumorigenic programs by regulating genes at post-transcriptional level. Long non-coding RNAs (lncRNAs) are novel targets for miRNAs. Here, we reported that miR-203 down-regulation was closely linked to advanced clinical features and poor overall survival (OS) of patients with hepatocellular carcinoma. We also confirmed that miR-203 and oncogene ADAM9 (a disintegrin and metalloproteinase 9)/oncogenic long non-coding RNA HULC (highly up-regulated in liver cancer) were inversely expressed in hepatocellular carcinoma (HCC) tissues or cell lines. More intriguingly, up-regulation of miR-203 diminished the expression of ADAM9 and HULC in HCC cancer cells. Over-expression of miR-203 could markedly inhibit cell proliferation, invasion and induce cell apoptosis. Furthermore, we identified that miR-203 modulated ADAM9 and HULC in a novel post-transcriptional regulatory mechanism. Over-expression of HULC partly rescued the miR-203-mediated antitumor effects. These results suggested that miR-203 played tumor suppressive roles by downregulating ADAM9 and HULC and indicated its potential application in cancer treatment.
authors.forename: Daiwei
authors.initials: D
authors.lastname: Wan
authors.forename: Shunli
authors.initials: S
authors.lastname: Shen
authors.forename: Shunjun
authors.initials: S
authors.lastname: Fu
authors.forename: Burnley
authors.initials: B
authors.lastname: Preston
authors.forename: Coder
authors.initials: C
authors.lastname: Brandon
authors.forename: Songbing
authors.initials: S
authors.lastname: He
authors.forename: Chenglong
authors.initials: C
authors.lastname: Shen
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Sutong
authors.initials: S
authors.lastname: Wang
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Xie
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Liya
authors.forename: Yixing
authors.initials: Y
authors.lastname: Guo
authors.forename: Dingcheng
authors.initials: D
authors.lastname: Zheng
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
authors.affiliation: Department of Hepatic Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510006, Guangdong, China. pengbaogang1963@sohu.com.
authors.forename: Baogang
authors.initials: B
authors.lastname: Peng
mesh_terms: D051722:ADAM Proteins / Q000172:deficiency* / Q000235:genetics; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C574685:HULC long non-coding RNA, human; C546703:MIRN203 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D051722:ADAM Proteins; C098339:ADAM9 protein, human