title: Potential of inherent RGD containing silk fibroin-poly (Є-caprolactone) nanofibrous matrix for bone tissue engineering.
abstract: The current study deals with the fabrication and characterization of blended nanofibrous scaffolds of tropical tasar silk fibroin of Antheraea mylitta and poly (Є-caprolactone) to act as an ideal scaffold for bone regeneration. The use of poly (Є-caprolactone) in osteogenesis is well-recognized. At the same time, the osteoconductive nature of the non-mulberry tasar fibroin is also established due to its internal integrin binding peptide RGD (Arg-Gly-Asp) sequences, which enhance cellular interaction and proliferation. Considering that the materials have the required and favorable properties, the blends are formed using an equal volume ratio of fibroin (2 and 4 wt%) and poly (Є-caprolactone) solution (10 wt%) to fabricate nanofibers. The nanofibers possess an average diameter of 152 ± 18 nm (2 % fibroin/PCL) and 175 ± 15 nm (4% fibroin/PCL). The results of Fourier transform infrared spectroscopy substantiates the preservation of the secondary structure of the fibroin in the blends indicating the structural stability of the neo-matrix. With an increase in the fibroin percentage, the hydrophobicity and thermal stability of the matrices as measured from melting temperature Tm (using DSC) decrease, while the mechanical strength is improved. The blended nanofibrous scaffolds are biodegradable, and support the viability and proliferation of human osteoblast-like cells as observed through scanning electron and confocal microscopes. Alkaline phosphatase assay indicates the cell proliferation and the generation of the neo-bone matrix. Taken together, these findings illustrate that the silk-poly (Є-caprolactone) blended nanofibrous scaffolds have an excellent prospect as scaffolding material in bone tissue engineering.
authors.affiliation: Materials Science Centre, Indian Institute of Technology Kharagpur, West Bengal, 721302, India.
authors.forename: Promita
authors.initials: P
authors.lastname: Bhattacharjee
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, 721302, India.|Institute of Tissue Regeneration Engineering (ITREN) & Department of Nanobiomedical Science BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, South Korea.
authors.forename: Banani
authors.initials: B
authors.lastname: Kundu
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, 721302, India.
authors.forename: Deboki
authors.initials: D
authors.lastname: Naskar
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN) & Department of Nanobiomedical Science BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, South Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Materials Science Centre, Indian Institute of Technology Kharagpur, West Bengal, 721302, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Bhattacharya
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, 721302, India. tkmaiti@hijli.iitkgp.ernet.in.
authors.forename: T K
authors.initials: TK
authors.lastname: Maiti
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, 721302, India. kundu@hijli.iitkgp.ernet.in.
authors.forename: S C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D019286:Endopeptidase K / Q000378:metabolism; D005349:Fibroins / Q000494:pharmacology*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D013696:Temperature; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D009842:Oligopeptides; D011091:Polyesters; C016240:polycaprolactone; C047981:arginyl-glycyl-aspartic acid; D005349:Fibroins; D000469:Alkaline Phosphatase; D019286:Endopeptidase K
keywords: Bone tissue engineering; Nanofibers; Non-mulberry silk fibroin; Poly (Є-caprolactone)