title: Synthetic RNAs Mimicking Structural Domains in the Foot-and-Mouth Disease Virus Genome Elicit a Broad Innate Immune Response in Porcine Cells Triggered by RIG-I and TLR Activation.
abstract: The innate immune system is the first line of defense against viral infections. Exploiting innate responses for antiviral, therapeutic and vaccine adjuvation strategies is being extensively explored. We have previously described, the ability of small in vitro RNA transcripts, mimicking the sequence and structure of different domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs), to trigger a potent and rapid innate immune response. These synthetic non-infectious molecules have proved to have a broad-range antiviral activity and to enhance the immunogenicity of an FMD inactivated vaccine in mice. Here, we have studied the involvement of pattern-recognition receptors (PRRs) in the ncRNA-induced innate response and analyzed the antiviral and cytokine profiles elicited in swine cultured cells, as well as peripheral blood mononuclear cells (PBMCs).
authors.affiliation: Centro de Investigación en Sanidad Animal, CISA-INIA, Valdeolmos, Madrid 28130, Spain. borrego@inia.es.
authors.forename: Belén
authors.initials: B
authors.lastname: Borrego
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CISC-UAM), Cantoblanco, Madrid 28049, Spain. mrrodriguez@cbm.csic.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Rodríguez-Pulido
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, Madrid 28040, Spain. crevilla@inia.es.
authors.forename: Concepción
authors.initials: C
authors.lastname: Revilla
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, Madrid 28040, Spain. avega@inia.es.
authors.forename: Belén
authors.initials: B
authors.lastname: Álvarez
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CISC-UAM), Cantoblanco, Madrid 28049, Spain. fsobrino@cbm.csic.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Sobrino
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, Madrid 28040, Spain. juncal@inia.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Domínguez
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CISC-UAM), Cantoblanco, Madrid 28049, Spain. msaiz@cbm.csic.es.
authors.forename: Margarita
authors.initials: M
authors.lastname: Sáiz
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000276:immunology*; D005260:Female; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D051379:Mice; D012367:RNA, Viral / Q000008:administration & dosage / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D013552:Swine; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D051193:Toll-Like Receptors; D014765:Viral Vaccines; D053487:DEAD-box RNA Helicases
keywords: antivirals; foot-and-mouth disease virus; innate immunity; non-coding RNA; viral sensors