title: miR-29bis an indicator of prognosis in breast cancer patients.
abstract: MicroRNA-29b (miR-29b) targets numerous important genes that mediate carcinogenesis and tumor development in breast cancerin vitroandin vivo. The aim of the present study was to determine the clinical significance ofmiR-29bexpression in primary breast cancer patients. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ofmiR-29band certain target genes ofmiR-29b, such as DNA methyltransferase 3A (DNMT3A), ten-eleven translocation 1 (TET1) and thymine DNA glycosylase (TDG), was performed in 94 primary breast cancer samples. Low expression ofmiR-29bin primary tumors was significantly associated with poorer disease-free survival (DFS) (P=0.0075) and overall survival (OS) (p=0.0012). Multivariate analysis indicated thatmiR-29bexpression was an independent prognostic factor for OS [relative risk=15.6 (2.33-348), P=0.0026]. In addition, a significant inverse correlation was identified between the expression levels ofDNMT3AandmiR-29bin estrogen receptor-positive breast cancer patients (P=0.027). To the best of our knowledge, this is the first study to investigate the clinicopathological significance ofmiR-29bin breast cancer cases andmiR-29bis shown to act as a tumor suppressive microRNA in breast cancer and as a potential marker for recurrence and metastasis in breast cancer patients.
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan ; Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Kagoshima 890-8520, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Shinden
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Iguchi
authors.affiliation: National Hospital Organization Kyushu Cancer Center, Fukuoka, Fukuoka 811-1395, Japan.
authors.forename: Sayuri
authors.initials: S
authors.lastname: Akiyoshi
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ueo
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Hidenari
authors.initials: H
authors.lastname: Hirata
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Sakimura
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Uchi
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takano
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Eguchi
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Sugimachi
authors.affiliation: Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Kagoshima 890-8520, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kijima
authors.affiliation: Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Kagoshima 890-8520, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Natsugoe
authors.affiliation: Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita 874-0838, Japan.
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
keywords: DNA methyltransferase 3A; breast cancer; clinicopathological significance; microRNA-29b; prognosis