title: G15 sensitizes epithelial breast cancer cells to doxorubicin by preventing epithelial-mesenchymal transition through inhibition of GPR30.
abstract: Resistance to single or multiple chemotherapeutic drugs is a major obstacle in breast cancer therapy. Recent studies have suggested that GPR30 is implicated in mediating cancer cell proliferation. The aim of this study was to examine the anti-tumor effects of the GPR30 antagonist G15 in breast cancer. We found that low concentrations of G15 had little effect on breast cancer cell viability, but could enhance doxorubicin sensitivity in MDA-MB-231 and MCF-7 cells with epithelial phenotypes. In addition, G15 prevented epithelial breast cancer cells undergoing epithelial-mesenchymal transition (EMT) after doxorubicin induction. Moreover, downregulation of GPR30 suppressed the EMT in breast cancer cells. These results support that G15 enhanced doxorubicin sensitivity and prevented the EMT in epithelial breast cancer cells by inhibiting GPR30 expression.
authors.affiliation: Department of Breast Surgery Center, The First Affiliated Hospital, School of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Fei-Ya
authors.initials: FY
authors.lastname: Du
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Breast Surgery Center, The First Affiliated Hospital, School of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Pei-Fen
authors.initials: PF
authors.lastname: Fu
authors.affiliation: Department of Breast Surgery Center, The First Affiliated Hospital, School of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Min-Ya
authors.initials: MY
authors.lastname: Yao
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Key Laboratory of Multi-Organ Transplantation of Ministry of Public Health Hangzhou, China.
authors.forename: Shu-Sen
authors.initials: SS
authors.lastname: Zheng
keywords: Breast cancer; G15; GPR30; epithelial-mesenchymal transition