title: Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.
abstract: This study was to investigate the effects of intracerebral hemorrhage (ICH) and subsequent minimally invasive hematoma aspiration on the expression of apoptosis-related genes in rats. IV-collagenase was injected to the caudate nucleus of the rats to make ICH models. In the control group, 30 Sprague-Dawley (SD) rats were mock treated with saline instead of collagenase. Thirty SD rats with successful modeling were designated as the ICH group. Twenty-five SD rats with successful modeling and subsequent minimally invasive hematoma aspiration were designated as the therapy group. Expression of heat shock protein 70 (Hsp70), B-cell leukemia/lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) in the brain tissues was detected by immunohistochemical assays. The expression of Hsp70, Bcl-2 and Bax in the control group was very low, and significantly increased in the ICH group and the therapy group. At each indicated time point, Hsp70 expression in the therapy group was significantly lower than that of the ICH group, Bax expression in the therapy group was significantly lower than that of the ICH group and Bcl-2 expression in the therapy group was significantly higher than that of the ICH group. These results suggest that ICH led to increased expression of apoptosis-related genes in the brain tissues. Hematoma aspiration up-regulated ICH induced Bcl-2 expression while down-regulated ICH induced Hsp70 and Bax expression.
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong Province, P. R. China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Heze Municipal Hospital Heze 274031, Shandong Province, P. R. China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Neurology, The Sixth People's Hospital of Jinan Jinan 250200, Shandong Province, P. R. China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong Province, P. R. China.
authors.forename: Jincun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong Province, P. R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Qilu Hospital of Shandong University Jinan 250012, Shandong Province, P. R. China ; Key Laboratory for Experimental Teratology of The Ministry of Education, Brain Science Research Institute, Shandong University Jinan 250012, Shandong Province, P. R. China.
authors.forename: Chuanzhu
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong Province, P. R. China.
authors.forename: Yuanxiao
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D017364:Collagenases; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006406:Hematoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013396:Suction; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D018840:HSP70 Heat-Shock Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D017364:Collagenases
keywords: Bax; Bcl-2; Hsp70; Intracerebral hemorrhage; minimally invasive hematoma aspiration