title: Insights into copper effect on Proteus hauseri through proteomic and metabolic analyses.
abstract: This is the first-attempt to use liquid chromatography coupled with tandem mass (LC-MS-MS) in deciphering the effects of copper ion on Proteus hauseri. Total 941 proteins in copper-addition (+Cu) group and 898 proteins in non-copper-addition (-Cu) group were found, which containing 221 and 178 differential proteins in +Cu and -Cu group, respectively. Differential proteins in both groups were defined into 14 groups by their functional classification which transport/membrane function proteins were the major different part between the two groups, which took 19.5% and 7.7%, respectively. The result of BioCyc and KEGG analyses on metabolic pathway indicated that copper could interrupted the pathway of chemotaxis CheY and inhibited the swarming of P. hauseri, which provided a potential in controlling the pathogenicity of this strain.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan; Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 70101, Taiwan. Electronic address: yswu@mail.ncku.edu.tw.
authors.forename: I-Son
authors.initials: IS
authors.lastname: Ng
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Chiming
authors.initials: C
authors.lastname: Ye
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Yuzhe
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemical and Materials Engineering, National I-Lan University, I-Lan, Taiwan.
authors.forename: Bor-Yann
authors.initials: BY
authors.lastname: Chen
mesh_terms: D018533:Biomass; D002633:Chemotaxis / Q000187:drug effects; D002853:Chromatography, Liquid; D003300:Copper / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D020543:Proteome / Q000145:classification / Q000187:drug effects*; D040901:Proteomics; D011511:Proteus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism* / Q000472:pathogenicity; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome; D003300:Copper; D010088:Oxidoreductases
keywords: Copper; Metabolic; Motility; Proteomics; Proteus hauseri