title: High-speed scanning electrochemical microscopy method for substrate kinetic determination: application to live cell imaging in human cancer.
abstract: Scanning electrochemical microscopy (SECM) is increasingly applied to study and image live cells. Quantitative analyses of biological systems, however, still remain challenging. In the presented study, single human adenocarcinoma cervical cancer cells are electrochemically investigated by means of SECM. The target cell's electrochemical response is observed over time under the influence of green tea catechins (GTC), which are suggested to offer chemopreventive and therapeutic effects on cancer. The electrochemical response of living target cells is measured experimentally and quantified in an apparent heterogeneous rate constant by using a numerical model, based on forced convection during high speed SECM imaging. The beneficial effect of GTC on cancer cells could be confirmed by SECM, and the presented study shows an alternative approach toward unraveling the mechanisms involved during inhibition of carcinogenesis.
authors.affiliation: †McGill University, Chemistry Department, 801 Sherbrooke Street W., Montreal, Québec H3A 2A7, Canada.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kuss
authors.affiliation: ‡Université de la Rochelle, Laboratoire des Sciences de l'Ingénieur Pour l'Environnement UMR-7536 CNRS, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Dao
authors.initials: D
authors.lastname: Trinh
authors.affiliation: †McGill University, Chemistry Department, 801 Sherbrooke Street W., Montreal, Québec H3A 2A7, Canada.
authors.forename: Janine
authors.initials: J
authors.lastname: Mauzeroll
mesh_terms: D063646:Carcinogenesis / Q000187:drug effects; D002392:Catechin / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004563:Electrochemistry; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D064347:Microscopy, Electrochemical, Scanning; D009369:Neoplasms / Q000473:pathology*; D013662:Tea / Q000737:chemistry
chemical_list: D013662:Tea; D002392:Catechin