title: Phytochemical properties and anti-proliferative activity of Olea europaea L. leaf extracts against pancreatic cancer cells.
abstract: Olea europaea L. leaves are an agricultural waste product with a high concentration of phenolic compounds; especially oleuropein. Oleuropein has been shown to exhibit anti-proliferative activity against a number of cancer types. However, they have not been tested against pancreatic cancer, the fifth leading cause of cancer related death in Western countries. Therefore, water, 50% ethanol and 50% methanol extracts of Corregiola and Frantoio variety Olea europaea L. leaves were investigated for their total phenolic compounds, total flavonoids and oleuropein content, antioxidant capacity and anti-proliferative activity against MiaPaCa-2 pancreatic cancer cells. The extracts only had slight differences in their phytochemical properties, and at 100 and 200 μg/mL, all decreased the viability of the pancreatic cancer cells relative to controls. At 50 μg/mL, the water extract from the Corregiola leaves exhibited the highest anti-proliferative activity with the effect possibly due to early eluting HPLC peaks. For this reason, olive leaf extracts warrant further investigation into their potential anti-pancreatic cancer benefits.
authors.affiliation: Nutrition Food & Health Research Group, School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. chloe.d.goldsmith@uon.edu.au.
authors.forename: Chloe D
authors.initials: CD
authors.lastname: Goldsmith
authors.affiliation: Nutrition Food & Health Research Group, School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. vanquan.vuong@newcastle.edu.au.
authors.forename: Quan V
authors.initials: QV
authors.lastname: Vuong
authors.affiliation: School of Biomedical Sciences and Pharmacy, University of Newcastle, Ourimbah, NSW 2258, Australia. elham.sadeqzadeh@newcastle.edu.au.
authors.forename: Elham
authors.initials: E
authors.lastname: Sadeqzadeh
authors.affiliation: Faculty of Bioscience Engineering, Ghent University Global Campus, Incheon 406-840, Korea. costas.stathopoulos@ghent.ac.kr.
authors.forename: Costas E
authors.initials: CE
authors.lastname: Stathopoulos
authors.affiliation: Nutrition Food & Health Research Group, School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. paul.roach@newcastle.edu.au.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Roach
authors.affiliation: Nutrition Food & Health Research Group, School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. c.scarlett@newcastle.edu.au.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Scarlett
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry; D031658:Olea / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D005419:Flavonoids; D057888:Iridoid Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; C002769:oleuropein
keywords: Olea europaea L.; antioxidant activity; biophenols; oleuropein; olive leaf; pancreatic cancer; phenolic compounds; phytochemicals