title: Cervical squamous cancer mRNA profiles reveal the key genes of metastasis and invasion.
abstract: OBJECTIVE
  To investigate mRNA expression profiles associated with cervical squamous carcinoma progression and to identify key genes involved in invasion and metastasis of cervical squamous cancer.
  
  
  METHODS
  The authors extracted the mRNA expression profile of eight normal cervical tissues by human whole genome microarray. The main functions of differentially expressed genes were identified by gene ontology (GO) analysis. Gene-networks were established based on bioinformatic approaches. Microarray data of the expressions level of key genes verified by qRT-PCR.
  
  
  RESULTS
  The authors identified 2036 differentially expressed genes between two groups including 1,282 down-regulated genes and 754 up-regulated genes (p < 0.05, FDR < 0.05). Gene-network revealed that PDGFRA, CAV1, and GJA-1 were critical for cervical cancer invasion and metastasis.
  
  
  CONCLUSIONS
  PDGFRA, CAV1, and GJA-1 were revealed as key node genes for cervical cancer invasion and metastasis. The results may provide new evidences and ideas for early diagnosis and prognosis assessment of cervical cancer.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Geng
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D051242:Caveolin 1 / Q000235:genetics*; D018031:Connexin 43 / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D046228:Microarray Analysis; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D030361:Papillomavirus Infections / Q000150:complications; D012333:RNA, Messenger / Q000378:metabolism*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D018031:Connexin 43; C500216:GJA1 protein, human; D012333:RNA, Messenger; D020796:Receptor, Platelet-Derived Growth Factor alpha