title: LC-MS/MS determination of 1-O-acetylbritannilactone in rat plasma and its application to a preclinical pharmacokinetic study.
abstract: A novel, rapid and sensitive LC-MS/MS method for the determination of 1-O-Acetylbritannilactone (ABL), a sesquiterpene lactone abundant in Inula britannica, was developed and validated using heteroclitin D as internal standard. Separation was achieved on a reversed phase Hypersil Gold C18 column (50 × 4.6 mm, i.d., 3.0 µm) using isocratic elution with methanol-5 mM ammonium acetate buffer aqueous solution (80:20, v/v) at a flow rate of 0.4 mL/min. Calibration curve was linear (r > 0.99) in a concentration range of 1.60-800 ng/mL with the lower limit of quantification of 1.60 ng/mL. Intra- and inter-day accuracy and precision were validated by relative error (RE) and relative standard deviation (RSD) values, respectively, which were both less than ±15%. The validated method has been successfully applied to a pharmacokinetic study of ABL in rats. The elimination half-lives were 0.412 ± 0.068, 0.415 ± 0.092 and 0.453 ± 0.071 h after a single intravenous administration of 0.14, 0.42, and 1.26 mg/kg ABL, respectively. The area under the plasma concentration-time curve from time zero to the last quantifiable time point and from time zero to infinity and the plasma concentrations at 2 min were linearly related to the doses tested.
authors.affiliation: Cadres Ward, Air Force General Hospital, PLA, Beijing, 100142, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurology, Affiliated Hospital of Beihua University, Jilin, 132011, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Health Center, 9524 Command, PLA, Beijing, 100195, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Neurology, Affiliated Hospital of Beihua University, Jilin, 132011, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D004355:Drug Stability; D007783:Lactones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007783:Lactones; C487680:acetylbritannilatone
keywords: 1-O-acetylbritannilactone; LC-MS/MS; pharmacokinetic study