title: Pharmacokinetic study of ofloxacin enantiomers in Pagrosomus major by chiral HPLC.
abstract: (S)-(-)-Ofloxacin and (R)-(+)-ofloxacin concentrations in the plasma of Pagrosomus major after drug treatment were detected by chiral high-performance liquid chromatography, and various pharmacokinetic parameters were calculated from these data. The elimination half-life of (S)-(-)-ofloxacin was significantly shorter than that of the (R)-(+) enantiomer. (S)-(-)-Ofloxacin also had a significantly lower maximum plasma concentration, area under the concentration-time curve from zero to infinity, and mean residence time than (R)-(+)-ofloxacin. However, the apparent volume of distribution and total body clearance of (S)-(-)-ofloxacin were greater than those of (R)-(+)-ofloxacin. The ratio of the (S)-(-)- to (R)-(+)-ofloxacin plasma concentration was always <1.0. Together, these data suggest that (S)-(-)-ofloxacin was preferentially excreted and (R)-(+)-ofloxacin was preferentially absorbed. Although the difference in pharmacokinetic parameters was small, the metabolic behavior of the ofloxacin enantiomers in P. major was enantioselective.
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.affiliation: Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.|School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Yang-Guang
authors.initials: YG
authors.lastname: Wang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.|School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: OuYang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Li-Ye
authors.initials: LY
authors.lastname: Yang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Yu
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Xu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015242:Ofloxacin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D021541:Sea Bream; D013237:Stereoisomerism
chemical_list: D015242:Ofloxacin
keywords: Pagrosomus major; enantioselective behavior; ofloxacin enantiomer; pharmacokinetic