title: Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components.
abstract: Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affinities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, One Medical Center Drive, 7927 Rubin Building, Lebanon, NH, 03756, USA, shohreh.farzan@dartmouth.edu.
authors.forename: Shohreh F
authors.initials: SF
authors.lastname: Farzan
authors.forename: David J
authors.initials: DJ
authors.lastname: Robbins
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D004330:Drosophila; D053823:Hedgehog Proteins / Q000378:metabolism*; D015151:Immunoblotting / Q000379:methods / Q000592:standards; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; D053823:Hedgehog Proteins; D011993:Recombinant Fusion Proteins