title: Comparative proteomic analysis of surface proteins of Trichinella spiralis muscle larvae and intestinal infective larvae.
abstract: The critical step for Trichinella spiralis infection is that muscle larvae (ML) are activated to intestinal infective larvae (IIL) and invade intestinal epithelium to further develop. The IIL is its first invasive stage, surface proteins are directly exposed to host environment and are crucial for larval invasion and development. In this study, shotgun LC-MS/MS was used to analyze surface protein profiles of ML and IIL. Totally, 41 proteins common to both larvae, and 85 ML biased and 113 IIL biased proteins. Some proteins (e.g., putative scavenger receptor cysteine-rich domain protein and putative onchocystatin) were involved in host-parasite interactions. Gene ontology analysis revealed that proteins involved in generation of precursor metabolites and energy; and nucleobase, nucleoside, nucleotide and nucleic acid metabolic process were enriched in IIL at level 4. Some IIL biased proteins might play important role in larval invasion and development. qPCR results confirmed the high expression of some genes in IIL. Our study provides new insights into larval invasion, host-Trichinella interaction and for screening vaccine candidate antigens.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Ruo Dan
authors.initials: RD
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China. Electronic address: cuij@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xiao Lin
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Ge Ge
authors.initials: GG
authors.lastname: Sun
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Shao Rong
authors.initials: SR
authors.lastname: Long
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China. Electronic address: wangzq@zzu.edu.cn.
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D006790:Host-Parasite Interactions; D007422:Intestines / Q000469:parasitology; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000469:parasitology; D016133:Polymerase Chain Reaction; D040901:Proteomics; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D053719:Tandem Mass Spectrometry; D017160:Trichinella spiralis / Q000235:genetics* / Q000378:metabolism; D014235:Trichinellosis / Q000469:parasitology*
chemical_list: D015801:Helminth Proteins; D008565:Membrane Proteins
keywords: Intestinal infective larvae; Muscle larvae; Surface proteins; Trichinella spiralis; proteome