title: Continuous Wavelet Transform, a powerful alternative to Derivative Spectrophotometry in analysis of binary and ternary mixtures: A comparative study.
abstract: A comparative study was established between two signal processing techniques showing the theoretical algorithm for each method and making a comparison between them to indicate the advantages and limitations. The methods under study are Numerical Differentiation (ND) and Continuous Wavelet Transform (CWT). These methods were studied as spectrophotometric resolution tools for simultaneous analysis of binary and ternary mixtures. To present the comparison, the two methods were applied for the resolution of Bisoprolol (BIS) and Hydrochlorothiazide (HCT) in their binary mixture and for the analysis of Amlodipine (AML), Aliskiren (ALI) and Hydrochlorothiazide (HCT) as an example for ternary mixtures. By comparing the results in laboratory prepared mixtures, it was proven that CWT technique is more efficient and advantageous in analysis of mixtures with severe overlapped spectra than ND. The CWT was applied for quantitative determination of the drugs in their pharmaceutical formulations and validated according to the ICH guidelines where accuracy, precision, repeatability and robustness were found to be within the acceptable limit.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
authors.forename: Eman S
authors.initials: ES
authors.lastname: Elzanfaly
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: said.hassan@pharma.cu.edu.eg.
authors.forename: Said A
authors.initials: SA
authors.lastname: Hassan
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
authors.forename: Maissa Y
authors.initials: MY
authors.lastname: Salem
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
authors.forename: Badr A
authors.initials: BA
authors.lastname: El-Zeany
mesh_terms: D000465:Algorithms; D000577:Amides / Q000032:analysis; D017311:Amlodipine / Q000032:analysis; D017298:Bisoprolol / Q000032:analysis; D045424:Complex Mixtures / Q000032:analysis; D005650:Fumarates / Q000032:analysis; D006852:Hydrochlorothiazide / Q000032:analysis; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D059629:Signal-To-Noise Ratio; D013053:Spectrophotometry / Q000379:methods*; D058067:Wavelet Analysis
chemical_list: D000577:Amides; D045424:Complex Mixtures; D005650:Fumarates; D006852:Hydrochlorothiazide; D017311:Amlodipine; C446481:aliskiren; D017298:Bisoprolol
keywords: Aliskiren; Amlodipine; Bisoprolol; Continuous Wavelet Transform; Derivative Spectrophotometry; Hydrochlorothiazide; Numerical Differentiation