title: Rapid changes in ovarian mRNA induced by brief photostimulation in Siberian hamsters (Phodopus sungorus).
abstract: This study sought to characterize the rapid intraovarian mRNA response of key folliculogenic factors that may contribute to the restoration of folliculogenesis during 2-10 days of photostimulation in Siberian hamsters. Adult hamsters were exposed to short photoperiod (8L:16D) for 14 weeks (SD). A subset were then transferred to long photoperiod (16L:8D) for 2 (PT day-2), 4 (PT day-4), or 10 days (PT day-10). Quantitative real-time PCR was used to measure intraovarian mRNA expression of: gonadotropin releasing hormone (GnRH), follicle stimulating hormone β-subunit (FSHβ-subunit), luteinizing hormone β-subunit (LHβ-subunit), FSH and LH receptors, estrogen receptors α and β (Esr1 and Esr2), matrix metalloproteinase (MMP)-2 and -9, anti-Müllerian hormone (AMH), inhibin-α subunit, fibroblast growth factor-2 (FGF-2) and proliferating cell nuclear antigen (PCNA). Compared to SD, plasma FSH concentrations increased on PT day-4 and the number of antral follicles and corpora lutea increased on PT day-10. FSHR and inhibin-α mRNA expression also increased on PT day-4, whereas LHR and proliferation marker PCNA both increased on PT day-10 as compared to SD. Esr1 mRNA increased on PT day-2 and remained significantly increased as compared to SD, whereas Esr1 mRNA increased only on PT day-2, similar to FGF-2 and MMP-2 results. No differences were observed in mRNA expression in ovarian GnRH, FSHβ- and LHβ-subunits, AMH, and MMP-9 mRNA with 2-10 days of photostimulation. Rapid increases in intraovarian FSHR and inhibin-α mRNA and antral follicle/corpora lutea numbers suggest that the ovary is primed to react quickly to the FSH released in response to brief periods of photostimulation.
authors.affiliation: Reproductive Biology Group, Department of Biological Sciences, California State University Long Beach, Long Beach, California.
authors.forename: Asha
authors.initials: A
authors.lastname: Shahed
authors.affiliation: Reproductive Biology Group, Department of Biological Sciences, California State University Long Beach, Long Beach, California.
authors.forename: Carling F
authors.initials: CF
authors.lastname: McMichael
authors.affiliation: Reproductive Biology Group, Department of Biological Sciences, California State University Long Beach, Long Beach, California.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Young
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000235:genetics / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D007265:Inhibins / Q000235:genetics / Q000378:metabolism; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D017111:Phodopus / Q000502:physiology*; D017440:Photoperiod; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism
chemical_list: D037101:Luteinizing Hormone, beta Subunit; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011987:Receptors, Steroid; C094377:inhibin-alpha subunit; D016222:Fibroblast Growth Factor 2; D007987:Gonadotropin-Releasing Hormone; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone; D020782:Matrix Metalloproteinases