title: Development of multiplexed bead arrays for the simultaneous detection of nucleic acid from multiple viruses in bat samples.
abstract: Virus surveillance of wildlife populations is important for identifying, monitoring, and predicting the emergence of pathogens that pose a potential threat to animal and human health. Bats are identified as important wildlife hosts of many viruses capable of causing fatal human disease, including members of the henipaviruses, coronaviruses, rhabdoviruses and filoviruses. As global warming and habitat change are thought to impact upon pathogen transmission dynamics and increase the risk of spillover, virus surveillance in bat populations remains a significant component of efforts to improve the prediction and control of potential future disease outbreaks caused by bat-borne viruses. In this study we have developed two fluid bead array assays containing customized panels that target multiple bat-borne viruses. These assays detect up to 11 viral RNA's simultaneously in urine samples collected from wild bat populations in Australia and Bangladesh. The assays developed show high specificity for the target viruses and the analytical sensitivity compares favorably to qRT-PCR. These assays enhance the ability to monitor multi-pathogen dynamics and identify patterns of virus shedding from bat populations, thus informing key approaches to outbreak response and control.
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: Vicky.Boyd@csiro.au.
authors.forename: Victoria
authors.initials: V
authors.lastname: Boyd
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: Ina.Smith@csiro.au.
authors.forename: Ina
authors.initials: I
authors.lastname: Smith
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: Gary.Crameri@csiro.au.
authors.forename: Gary
authors.initials: G
authors.lastname: Crameri
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: amy_in_paris@hotmail.com.
authors.forename: Amy L
authors.initials: AL
authors.lastname: Burroughs
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: Peter.Durr@csiro.au.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Durr
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: John.White@csiro.au.
authors.forename: John
authors.initials: J
authors.lastname: White
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: Chris.Cowled@csiro.au.
authors.forename: Christopher
authors.initials: C
authors.lastname: Cowled
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia. Electronic address: Glenn.Marsh@csiro.au.
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Marsh
authors.affiliation: CSIRO Biosecurity Flagship & Australian Animal Health Laboratory, Geelong, Victoria, Australia; Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School Singapore, Singapore. Electronic address: Linfa.Wang@csiro.au.
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000818:Animals; D001315:Australia; D001459:Bangladesh; D002685:Chiroptera / Q000821:virology*; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014556:Urine / Q000821:virology*; D014777:Virus Diseases / Q000662:veterinary* / Q000821:virology; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification*
keywords: Bats; Luminex; MagPlex-TAG; Multiplex; Virus surveillance