title: Protective properties of ginsenoside Rb1 against UV-B radiation-induced oxidative stress in human dermal keratinocytes.
abstract: Ginsenosides, also known as ginseng saponins, are responsible for most pharmacological effect of ginseng. Ginsenoside Rb1 (Rb1) exerts a variety of pharmacological properties, including anti-inflammatory, antistress, anti-aging and anti-neurodegenerative activities. The aim of the present work was to assess the skin anti-photoaging properties of Rb1 in human dermal keratinocyte HaCaT cells. The anti-photoaging activity was evaluated by analyzing the levels of reactive oxygen species (ROS) and matrix metalloproteinases (MMPs) as well as cell viability for HaCaT cells under UV-B irradiation. Rb1 was able to suppress the ROS levels which were elevated under UV-B irradiation, and unable to influence cellular survival in UV-B-irradiated HaCaT cells. Rb1 diminished the enhancement of MMP-2 gelatinolytic activity in conditioned medium, which corresponded with the decreased MMP-2 protein levels in both conditioned medium and cellular lysate prepared from UV-B-irradiated HaCaT cultures. Rb1 could restore the total glutathione (GSH) and superoxide dismutase (SOD) activity diminished in UV-B-irradiated HaCaT cells. Ginsenoside Rb1 possesses skin anti-photoaging properties through scavenging ROS and decreasing MMP-2 levels possibly by enhancing antioxidant activity in keratinocytes under UV-B irradiation.
authors.forename: Sun-Joo
authors.initials: SJ
authors.lastname: Oh
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Kim
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D036145:Ginsenosides / Q000494:pharmacology*; D005978:Glutathione / Q000096:biosynthesis; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000166:cytology; D013482:Superoxide Dismutase / Q000096:biosynthesis; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D036145:Ginsenosides; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C442759:ginsenoside Rb1; D013482:Superoxide Dismutase; D020778:Matrix Metalloproteinase 2; D005978:Glutathione