title: Comparison of multi-recognition molecularly imprinted polymers for recognition of melamine, cyromazine, triamterene, and trimethoprim.
abstract: Three fragmental templates, including 2,4-diamino-6-methyl-1,3,5-triazine (DMT), cyromazine (CYR), and trimethoprim (TME), were used to prepare the fragment molecularly imprinted polymers (FMIPs), respectively, in polar ternary porogen which was composed of ionic liquid ([BMIM]BF4), methanol, and water. The morphology, specific surface areas, and selectivity of the obtained FMIPs for fragmental analogues were systematically characterized. The experimental results showed that the FMIPs possessed the best specific recognition ability to the relative template and the greatest imprinting factor (IF) was 5.25, 6.69, and 7.11 of DMT on DMT-MIPs, CYR on CYR-MIPs, and TME on TME-MIPs, respectively. In addition, DMT-MIPs also showed excellent recognition capability for fragmental analogues including CYR, melamine (MEL), triamterene (TAT), and TME, and the IFs were 2.08, 3.89, 2.18, and 2.60, respectively. The effects of pH and temperature on the retention of the fragmental and structural analogues were studied in detail. Van't Hoff analysis indicated that the retention and selectivity on FMIPs were an entropy-driven process, i.e., steric interaction. The resulting DMT-MIPs were used as a solid-phase extraction material to enrich CYR, MEL, TAT, and TME in different bio-matrix samples for high-performance liquid chromatography analysis. The developed method had acceptable recoveries (86.8-98.6%, n = 3) and precision (2.7-4.6%) at three spiked levels (0.05-0.5 μg g(-1)).
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, 22 Qixiangtai Road, Heping District, Tianjin, 300070, China.
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Peng
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Zhao-Sheng
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045424:Complex Mixtures / Q000032:analysis / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D014223:Triamterene / Q000032:analysis* / Q000737:chemistry; D014227:Triazines / Q000032:analysis* / Q000737:chemistry; D014295:Trimethoprim / Q000032:analysis* / Q000737:chemistry
chemical_list: D045424:Complex Mixtures; D052578:Ionic Liquids; D011108:Polymers; D014227:Triazines; D014295:Trimethoprim; C028704:cyromazine; C011907:melamine; D014223:Triamterene