title: Fast Magnetic Field-Enhanced Linear Colloidal Agglutination Immunoassay.
abstract: We present the principle of a fast magnetic field enhanced colloidal agglutination assay, which is based on the acceleration of the recognition rate between ligands and receptors induced by magnetic forces. By applying a homogeneous magnetic field of 20 mT for only 7 s, we detect CRP (C-reactive protein) in human serum at a concentration as low as 1 pM for a total cycle time of about 1 min in a prototype analyzer. Such a short measurement time does not impair the performances of the assay when compared to longer experiments. The concentration range dynamic is shown to cover 3 orders of magnitude. An analytical model of agglutination is also successfully fitting our data obtained with a short magnetic pulse.
authors.affiliation: †HORIBA ABX SAS, Parc Euromédecine - Rue du Caducée, BP 7290, 34184 Montpellier CEDEX 4, France.|‡Laboratoire Colloïdes et Matériaux divisés ESPCI, 10 Rue Vauquelin, 75231 Paris, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Daynès
authors.affiliation: †HORIBA ABX SAS, Parc Euromédecine - Rue du Caducée, BP 7290, 34184 Montpellier CEDEX 4, France.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Temurok
authors.affiliation: †HORIBA ABX SAS, Parc Euromédecine - Rue du Caducée, BP 7290, 34184 Montpellier CEDEX 4, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Gineys
authors.affiliation: †HORIBA ABX SAS, Parc Euromédecine - Rue du Caducée, BP 7290, 34184 Montpellier CEDEX 4, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Cauet
authors.affiliation: †HORIBA ABX SAS, Parc Euromédecine - Rue du Caducée, BP 7290, 34184 Montpellier CEDEX 4, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Nerin
authors.affiliation: ‡Laboratoire Colloïdes et Matériaux divisés ESPCI, 10 Rue Vauquelin, 75231 Paris, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Baudry
authors.affiliation: ‡Laboratoire Colloïdes et Matériaux divisés ESPCI, 10 Rue Vauquelin, 75231 Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bibette
mesh_terms: D002097:C-Reactive Protein / Q000737:chemistry; D003102:Colloids / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007700:Kinetics; D057230:Limit of Detection; D008280:Magnetics
chemical_list: D003102:Colloids; D002097:C-Reactive Protein