title: Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms.
abstract: OBJECTIVE
  To characterize two Achromobacter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance program.
  
  
  METHODS
  Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and PCR.
  
  
  RESULTS
  Two Achromobacter xylosoxidans A8, closely related to Bordetella spp. were recovered from 2 patients diagnosed of pertussis, both carrying the ptxA gene and IS418 the pertussis toxin encoding gene. Subsequently, antibiotic susceptibility was evaluated by disk-diffusion method and by PCR.
  
  
  CONCLUSIONS
  Although more detailed studies are needed, the present data highlight the possibility that Achromobacter xylosoxidans, closely related Bordetella pertusssis microorganisms and not covered under the vaccine umbrella, might also result in cases of whooping cough. Thereby further surveillance is necessary to determine the extension and relevance of their pathogenic role in order to discriminate their real public health implication.
authors.affiliation: Centro de Investigación de la, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru.
authors.forename: Fiorella
authors.initials: F
authors.lastname: Orellana-Peralta
authors.affiliation: Centro de Investigación de la, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru.
authors.forename: Michelle
authors.initials: M
authors.lastname: Jacinto
authors.affiliation: Centro de Investigación de la, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Pons
authors.affiliation: ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Gomes
authors.affiliation: Hospital de Emergencias Pediatricas, Lima, Peru.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bada
authors.affiliation: Hospital de Emergencias Pediatricas, Lima, Peru.
authors.forename: Isabel
authors.initials: I
authors.lastname: Reyes
authors.affiliation: Centro de Investigación de la, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru; Instituto de Investigación Nutricional, Lima, Peru. Electronic address: jdelvall@upc.edu.pe.
authors.forename: Juana
authors.initials: J
authors.lastname: Del Valle Mendoza
authors.affiliation: ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain. Electronic address: joruiz@clinic.ub.es.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ruiz
keywords: Achromobacter xylosoxidans; Bordetella pertussis; Pertussis-like