title: [Functions of human periodontal myofibroblast in vitro].
abstract: OBJECTIVE
  To investigate the functions of human periodontal myofibroblast (MFB) in vitro.
  
  
  METHODS
  Human periodontal fibroblast (hPDLFs) was cultured and induced to MFB by transforming growth factor-β1 (TGF-β1). MFB was denoted as the experimental group, whereas the hPDLFs was the control group. The groups were continuously cultured and harvested at 0, 12, 24, 48, and 72 h. The MFB marker α-smooth muscle actin (α-SMA) was examined by immunocytochemistry. The expression of fibronectin (FN) between MFB was examined by immunocytochemistry to detect the MFB contact relationship. The mRNA expression levels of α-SMA, collagen (Col) I, and Col III were measured by reverse transcription-polymerase chain reaction (RT-PCT) to analyze extracellular matrix secretion. The protein expression levels of α-SMA and Col I were also assessed by Western blot.
  
  
  RESULTS
  The experimental group had significantly higher α-SMA expression than the control group at 0 h (P < 0.001). A positive expression of FN was found between MFB. The experimental group had significantly higher expression levels of Col I and Col III than the control group at 24 h (P < 0.001).
  
  
  CONCLUSIONS
  Human periodontal MFB presents a continuous, high expression of α-SMA. MFB could interact through FN. MFB is significantly capable of extracellular matrix secretion.
authors.forename: Meng
authors.initials: M
authors.lastname: Yao
authors.forename: Liu
authors.initials: L
authors.lastname: Man
authors.forename: Bai
authors.initials: B
authors.lastname: Ding
mesh_terms: D000199:Actins; D004847:Epithelial Cells; D005109:Extracellular Matrix; D005347:Fibroblasts; D005353:Fibronectins; D006801:Humans; D007568:Jaw / Q000378:metabolism*; D058628:Myofibroblasts; D053773:Transforming Growth Factor beta1
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D005353:Fibronectins; D053773:Transforming Growth Factor beta1