title: [MiR-373-3p Promotes Invasion and Metastasis of Lung Adenocarcinoma Cells].
abstract: OBJECTIVE
  Lung cancer is the leading cause of cancer-related deaths worldwide, and metastasis is the major cause of death in lung cancer patients. MiR-373 is closely associated with invasion and metastasis in other tumor cells. This study explored the expression of miR-373-3p in non-small cell lung cancer (NSCLC) and its effect on the invasive and metastatic capabilities of lung adenocarcinoma cells, as well as their mechanisms of action.
  
  
  METHODS
  The expression of miR-373-3p in NSCLC tissues and lung adenocarcinoma cell lines was detected by quantitative reverse transcription polymerase chain reaction. The roles of miR-373-3p in regulating lung adenocarcinoma cell invasion and metastatic properties were analyzed with miR-373-3p mimic/inhibitor-transfected cells via Transwell chamber assay. Matrix metalloproteinase MMP-9 and MMP-14 protein levels were detected by Western blot in lung cancer cells after transfection.
  
  
  RESULTS
  MiR-373-3p was upregulated in 51 NSCLC tissues and 5 NSCLC cell lines. Gain-of-function and loss-of-function studies showed that overexpression of miR-373-3p promoted H1299 cell migration and invasion, which resulted in upregulation of MMP-9 and MMP-14. By contrast, miR-373-3p knockdown inhibited these processes in A549 cells and downregulated the expression of MMP-9 and MMP-14.
  
  
  CONCLUSIONS
  Our results demonstrated that miR-373-3p participated in the invasion and metastasis of lung adenocarcinoma cells, partly by upregulation of MMP-9 and MMP-14.
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Aibing
authors.initials: A
authors.lastname: Wu
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Mo
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Haiyin
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Yahai
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Meilian
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524000, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis
chemical_list: C527114:MIRN373 microRNA, human; D035683:MicroRNAs; D020780:Matrix Metalloproteinase 9; D053511:Matrix Metalloproteinase 14