title: A simple HPLC-UV method for the quantification of theophylline in rabbit plasma and its pharmacokinetic application.
abstract: A simple, precise and accurate high-performance liquid chromatography-ultraviolet method was developed and validated for the quantification of theophylline in rabbit plasma using hydroxyethyl theophylline as an internal standard. Separation was performed on Waters(®) C18 column (µBondapak™ 5 µm, 150 × 3.9 mm) using a mobile phase consisting of water-acetonitrile (96:4 v/v) at a flow rate of 1 mL/min. Validation of the method was performed in order to demonstrate its selectivity, linearity, precision, accuracy and stability. The calibration curves of theophylline were linear over a concentration range of 0.1-25 µg/mL. The within- and between-day coefficient of variation (CV) were <10%. The extraction recoveries of theophylline at the three levels of quality control samples were 63.1, 69.4 and 69.7%. The method was rapid with retention time of theophylline and the internal standard observed at ∼5.2 and 6.5 min, respectively. The developed method was applied successfully for studying the pharmacokinetics of theophylline in rabbits.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia aljenobi@ksu.edu.sa.
authors.forename: Fahad I
authors.initials: FI
authors.lastname: Al-Jenoobi
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Abdul
authors.initials: A
authors.lastname: Ahad
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Gamal M
authors.initials: GM
authors.lastname: Mahrous
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Raish
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Mohd Aftab
authors.initials: MA
authors.lastname: Alam
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Al-Mohizea
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013806:Theophylline / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D013806:Theophylline