title: Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence.
abstract: Mediator is a conserved multi-protein complex that acts as a bridge between promoter-bound transcriptional regulators and RNA polymerase II. While redox signaling is important in adjusting plant metabolism and development, the involvement of Mediator in redox homeostasis and regulation only recently started to emerge. Our previous results show that the MED10a, MED28, and MED32 Mediator subunits form various types of covalent oligomers linked by intermolecular disulfide bonds in vitro. To link that with biological significance we have characterized Arabidopsis med32 and med28 mutants and found that they are affected in root development and senescence, phenotypes possibly associated to redox changes.
authors.affiliation: Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden. jehadgermany@gmail.com.
authors.forename: Jehad
authors.initials: J
authors.lastname: Shaikhali
authors.affiliation: Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden.|Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK, 73019, USA.
authors.forename: Céline
authors.initials: C
authors.lastname: Davoine
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå Plant Science Center, Umeå University, SE-901 87, Umeå, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Björklund
authors.affiliation: Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden. Gunnar.Wingsle@slu.se.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Wingsle
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; C000612887:MED28 protein, Arabidopsis; D056892:Mediator Complex; D006861:Hydrogen Peroxide
keywords: Mediator; Oligomerization; Redox; Root development; Senescence