title: Preparation and therapeutic application of docetaxel-loaded poly(d,l-lactide) nanofibers in preventing breast cancer recurrence.
abstract: The aim of this study was to develop docetaxel (DTX)-loaded poly-d,l-lactide (PDLLA) nanofibers and evaluate their therapeutic effect in preventing local breast cancer recurrence. DTX was incorporated into biodegradable PDLLA nanofibers by electrospinning. The surface morphology of the DTX/PDLLA nanofibers was characterized using scanning electron microscopy and wide angle X-ray diffraction. The in vitro release behavior of DTX from the fiber mats was also studied in detail. The cytotoxicity of DTX/PDLLA nanofibers was evaluated by MTT assay in 4T1 breast cancer cells. Flow cytometry revealed that DTX/PDLLA nanofibers exhibited apoptotic activity in 4T1 cells. In vivo antitumor efficacy of DTX/PDLLA nanofibers was evaluated in BALB/c mice bearing local breast tumors. Locoregional recurrence after primary tumor resection decreased obviously in mice treated with subcutaneously (16.7%) administered DTX-loaded PDLLA nanofibers, compared with the blank PDLLA nanofibers (88.9%), systemic (75.0%) or locally (77.8%) administered DTX and the control group (100%) (p < 0.05). Finally, after subcutaneous transplantation in mice, the DTX/PDLLA scaffolds presented excellent biocompatibility, as exhibited by the minimal presence of inflammatory cells in the region surrounding the scaffolds. Our results suggest that DTX/PDLLA nanofibers could have great potential for clinical application requiring local chemotherapy.
authors.affiliation: a State Key Laboratory of Biotherapy and Cancer Center , West China Hospital, West China Medical School, Sichuan University , Chengdu , P.R. China.|b Departmentof Gynaecology and Obstetrics , Xinqiao Hospital, Third Military Medical University , Chongqing , China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Ding
authors.affiliation: c Department of Gastroenterology , Affiliated Hospital (T.C.M) of Luzhou Medical College, Luzhou Medical College , Luzhou , China , and.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Biotherapy and Cancer Center , West China Hospital, West China Medical School, Sichuan University , Chengdu , P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a State Key Laboratory of Biotherapy and Cancer Center , West China Hospital, West China Medical School, Sichuan University , Chengdu , P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.affiliation: a State Key Laboratory of Biotherapy and Cancer Center , West China Hospital, West China Medical School, Sichuan University , Chengdu , P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.affiliation: b Departmentof Gynaecology and Obstetrics , Xinqiao Hospital, Third Military Medical University , Chongqing , China.
authors.forename: Zhengqiong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: b Departmentof Gynaecology and Obstetrics , Xinqiao Hospital, Third Military Medical University , Chongqing , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a State Key Laboratory of Biotherapy and Cancer Center , West China Hospital, West China Medical School, Sichuan University , Chengdu , P.R. China.
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
authors.affiliation: d School of Ophthalmology & Optometry and Eye Hospital, Institute of Biomedical Engineering, Wenzhou Medical University , Wenzhou , China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001943:Breast Neoplasms / Q000517:prevention & control*; D045744:Cell Line, Tumor; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry*; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control*; D011091:Polyesters / Q000737:chemistry*; D043823:Taxoids / Q000008:administration & dosage* / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D011091:Polyesters; D043823:Taxoids; D000077143:Docetaxel; C033616:poly(lactide)
keywords: Breast cancer; PDLLA; chemotherapy; docetaxel; nanofibers; tumor recurrence