title: Characterization of Salmonella enterica Serovar 4,[5],12:i:- Isolates from Korean Food Animals.
abstract: Salmonella enterica subspecies enterica serovar 4,[5],12:i:-, a monophasic variant of Salmonella Typhimurium, has emerged as one of the most common serotypes related to human salmonellosis. In this study, the 22 isolates of S. 4,[5],12:i:- from food animals were identified by a specific multiplex polymerase chain reaction between 2009 and 2012. The isolation rate of S. 4,[5],12:i:- accounted for 1.7% (22/1271) of Salmonella spp. isolates from food animal origins: more specifically, 7.6% (18/235) from pigs and 0.6% (4/686) from chickens. The predominant S. 4,[5],12:i:- isolates in Korea belonged to phage type DT193 (12/22) with ampicillin-streptomycin-sulfonamide-tetracycline (ASSuT) resistance pattern (9/22). The XbaI-pulsed-field gel electrophoresis (PFGE) analysis revealed 11 different pulsotypes, and the major X-1 pattern was shared by 8 isolates. The isolates belonging to pattern X-1 were further subdivided into three BlnI-PFGE patterns and four variable-number tandem-repeat analysis (MLVA) allele combinations. The combining of MLVA and PFGE data could be valuable in characterizing highly clonal strains and discriminating their epidemiological relationship.
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.|2 College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Aeran
authors.initials: A
authors.lastname: Kim
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.
authors.forename: Suk-Kyung
authors.initials: SK
authors.lastname: Lim
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.
authors.forename: Kichan
authors.initials: K
authors.lastname: Lee
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.
authors.forename: Suk-Chan
authors.initials: SC
authors.lastname: Jung
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.
authors.forename: Yun Sang
authors.initials: YS
authors.lastname: Cho
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.
authors.forename: Seon-Jong
authors.initials: SJ
authors.lastname: Yun
authors.affiliation: 2 College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.affiliation: 1 Animal and Plant Quarantine Agency , Anyang-si, Gyeonggi-do, Korea.
authors.forename: Byeong Yeal
authors.initials: BY
authors.lastname: Jung
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology; D002645:Chickens; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology; D056910:Republic of Korea; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents