title: The Effect of Electroporation of a Lyotroic Liquid Crystal Genistein-Based Formulation in the Recovery of Murine Melanoma Lesions.
abstract: A lamellar lyotropic liquid crystal genistein-based formulation (LLC-Gen) was prepared in order to increase the aqueous solubility of the lipophilic phytocompound genistein. The formulation was applied locally, in a murine model of melanoma, with or without electroporation. The results demonstrated that, when the formulation was applied by electroporation, the tumors appeared later. During the 21 days of the experiment, the LLC-Gen formulation decreased the tumor volume, the amount of melanin and the degree of erythema, but when electroporation was applied, all these parameters indicated a better prognosis even (lower tumor volume, amount of melanin and degree of erythema). Although hematoxylin-eosin (HE) staining confirmed the above events, application of the LLC-Gen formulation by electroporation did not lead to a significant effect in terms of the serum concentrations of the protein S100B and serum neuron specific enolase (NSE), or the tissue expression of the platelet-derived growth factor receptor β (PDGFRβ) antibody.
authors.affiliation: Department of Pharmacognosy, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. corina.danciu@umft.ro.
authors.forename: Corina
authors.initials: C
authors.lastname: Danciu
authors.affiliation: Department of Pharmaceutical Technology, University of Szeged, Szeged 6720, Hungary. berko@pharm.u-szeged.hu.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Berkó
authors.affiliation: Dr. Derm Clinic of Anti-Aging Dermatology, Aesthetic Laser and Plastic Surgery, Budapest 1026, Hungary. g.varju@drderm.net.
authors.forename: Gábor
authors.initials: G
authors.lastname: Varju
authors.affiliation: Department of Pharmaceutical Technology, University of Szeged, Szeged 6720, Hungary. balazs.boglarka@pharm.u-szeged.hu.
authors.forename: Boglárka
authors.initials: B
authors.lastname: Balázs
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged 6720, Hungary. kl@mail.derma.szote.u-szeged.hu.
authors.forename: Lajos
authors.initials: L
authors.lastname: Kemény
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged 6720, Hungary. nemeth.istvan.balazs@med.u-szeged.hu.
authors.forename: István Balázs
authors.initials: IB
authors.lastname: Németh
authors.affiliation: Department of Pathology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania. cioca_andre@yahoo.com.
authors.forename: Andreea
authors.initials: A
authors.lastname: Cioca
authors.affiliation: Department of Anatomy, Physiology and Pathophysiology, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. alexandra.petrus@umft.ro.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Petruș
authors.affiliation: Department of Toxicology, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. cadehelean@umft.ro.
authors.forename: Cristina
authors.initials: C
authors.lastname: Dehelean
authors.affiliation: Department of Obstetrics and Gynecology, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. citu.ioan@umft.ro.
authors.forename: Citu Ioan
authors.initials: CI
authors.lastname: Cosmin
authors.affiliation: Department of Rehabilitation, Physical Medicine and Rheumatology, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. ama.elena@gmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Amaricai
authors.affiliation: Department of Pharmacognosy, Western University "Vasile Goldis", 310025 Arad, Romania. claudiatoma2004@yahoo.com.
authors.forename: Claudia Crina
authors.initials: CC
authors.lastname: Toma
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000737:chemistry*; D018274:Electroporation / Q000379:methods*; D005260:Female; D019833:Genistein / Q000008:administration & dosage / Q000737:chemistry*; D007150:Immunohistochemistry; D050866:Liquid Crystals / Q000737:chemistry*; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010751:Phosphopyruvate Hydratase / Q000097:blood; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism; D012212:Rheology; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood; D012867:Skin / Q000378:metabolism / Q000473:pathology; D014184:Transplantation, Homologous; D014227:Triazines / Q000378:metabolism
chemical_list: D016588:Anticarcinogenic Agents; D004337:Drug Carriers; D064568:S100 Calcium Binding Protein beta Subunit; C579159:S100b protein, mouse; D014227:Triazines; D019833:Genistein; D020797:Receptor, Platelet-Derived Growth Factor beta; D010751:Phosphopyruvate Hydratase; C011907:melamine
keywords: electroporation; genistein; lyotropic liquid crystals; murine melanoma