title: Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination.
abstract: Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: You
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Yuanya
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Men
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Department of Nephrology and Rheumatology of Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200092, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Zhixue
authors.initials: Z
authors.lastname: Gan
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xie
authors.affiliation: Department of Science and Education, People's Hospital of Zunhua, Tangshan, Hebei 064200, P. R. China.
authors.forename: Jiangsheng
authors.initials: J
authors.lastname: Mi
authors.affiliation: Department of Science and Education, People's Hospital of Zunhua, Tangshan, Hebei 064200, P. R. China.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Science and Education, People's Hospital of Zunhua, Tangshan, Hebei 064200, P. R. China.
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Life and Environmental Sciences, Wenzhou University, Wenzhou, Zhejiang 325035, P. R. China.
authors.forename: Pei-Chao
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Chang
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Cai
authors.affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China; Department of Science and Education, People's Hospital of Zunhua, Tangshan, Hebei 064200, P. R. China.
authors.forename: Su
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002843:Chromatin / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D025801:Ubiquitin / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D002843:Chromatin; D006657:Histones; D050505:Mutant Proteins; D025801:Ubiquitin