title: Boron Nitride Nanoparticles with a Petal-Like Surface as Anticancer Drug-Delivery Systems.
abstract: Nanoparticles (NPs) have a great potential as nanosized drug-delivery carriers. Such systems must safely deliver the drug to the site of the tumor without drug leakage, effectively penetrate inside cancer cells, and provide intracellular drug release. Herein we developed an original and simple method aimed at the fabrication of spherical boron nitride NPs (BNNPs), 100-200 nm in diameter, with peculiar petal-like surfaces via chemical vapor deposition. Such structures were found to be able to absorb a large amount of antitumor drug-killing tumor cells. They revealed low cytotoxicity and rapid cellular uptake. BNNPs were saturated with doxorubicin (DOX) and then dispersed. The BNNPs loaded with DOX (BNNPs-DOX) were stable at neutral pH but effectively released DOX at pH 4.5-5.5. MTT assay and cell growth testing showed that the BNNPs-DOX nanocarriers had been toxic for IAR-6-1 cells. BNNPs loaded with DOX penetrated into the neoplastic IAR-6-1 cells using endocytic pathways, and then DOX released into the cytoplasm and cell nuclei and resulted in cell death.
authors.affiliation: †National University of Science and Technology "MISIS", Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Sukhorukova
authors.affiliation: ‡N. N. Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia.
authors.forename: Irina Y
authors.initials: IY
authors.lastname: Zhitnyak
authors.affiliation: †National University of Science and Technology "MISIS", Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: Andrey M
authors.initials: AM
authors.lastname: Kovalskii
authors.affiliation: †National University of Science and Technology "MISIS", Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: Andrei T
authors.initials: AT
authors.lastname: Matveev
authors.affiliation: ⊥CRISMAT, UMR 6508, CNRS-ENSICAEN, 6Bd Marechal Juin, 14050 Caen, France.
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Lebedev
authors.affiliation: ∥National Institute for Materials Science (NIMS), Namiki 1-1, Tsukuba, Ibaraki 3050044, Japan.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: ‡N. N. Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Gloushankova
authors.affiliation: ∥National Institute for Materials Science (NIMS), Namiki 1-1, Tsukuba, Ibaraki 3050044, Japan.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Golberg
authors.affiliation: †National University of Science and Technology "MISIS", Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Shtansky
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001896:Boron Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D001896:Boron Compounds; D004337:Drug Carriers; C017282:boron nitride; D004317:Doxorubicin
keywords: BN nanoparticles; chemical vapor deposition; doxorubicin; drug release; drug-delivery nanocarriers; neoplastic cells