title: The study of the oipA and dupA genes in Helicobacter pylori strains and their relationship with different gastroduodenal diseases.
abstract: OBJECTIVE
  The purpose of this investigation was to determine the oipA and dupA genes of Helicobacter pylori isolates from west of Iran; Chaharmahalo Bakhtiyari region and find their relationship with the severity of the gastroduodenal diseases.
  
  
  BACKGROUND
  Helicobacter pylori is an organism responsible for many gastroduodenal diseases. Many studies suggest that genetic diversity in H . pylori virulence factors such as oipA and dupA genes is high among isolates of different geographic regions and may cause more severe diseases.
  
  
  METHODS
  In this cross-sectional study, gastric biopsy specimens were taken from 150 patients suffering from gastroduodenal diseases. The presence of ureC, dupA and oipA genes was tested by polymerase chain reaction (PCR).
  
  
  RESULTS
  Overall, 123 (82%) H. pylori strains were isolated from 150 specimens. dupA gene was detected in 41 (33.33%) H.pylori-positive specimens. There was a reverse correlation between this gene and gastric cancer. The oipA gene was found in 88 (71.54%) samples and statistically there was no association between this gene and gastric disorders. As statistical analyses revealed, the presence of the dupA was more common in isolates with the oipA negative.
  
  
  CONCLUSIONS
  Based on our findings, the presence of dupA gene can be considered as a marker for the onset of severe diseases. However, the oipA gene cannot be regarded for prediction of gastroenterology diseases. Meanwhile, extended molecular epidemiology researches in other populations are recommended.
authors.affiliation: Young Researchers club, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Souod
authors.affiliation: Department of Public Health and Infectious Diseases, Sapienza, University of Rome, Rome, Italy.
authors.forename: Meysam
authors.initials: M
authors.lastname: Sarshar
authors.affiliation: Department of Clinical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Dabiri
authors.affiliation: Department of Microbiology, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Momtaz
authors.affiliation: Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kargar
authors.affiliation: Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Mohammadzadeh
authors.affiliation: Gastroenterology and Liver Diseases Research Centre, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Abdi
keywords: Gastric disorders; Helicobacter pylori; PCR; dupA; oipA