title: Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods.
abstract: Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry-intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, UK, steve.harding@nottingham.ac.uk.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Harding
authors.forename: Ali Saber
authors.initials: AS
authors.lastname: Abdelhameed
authors.forename: Richard B
authors.initials: RB
authors.lastname: Gillis
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Morris
authors.forename: Gary G
authors.initials: GG
authors.lastname: Adams
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D006001:Glycoconjugates / Q000737:chemistry*; D057446:Hydrodynamics; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D006001:Glycoconjugates; D011135:Polysaccharides, Bacterial