title: Concise Synthesis of Triazole-Linked 5'-Peptide-Oligonucleotide Conjugates by Click Chemistry.
abstract: A concise synthesis of oligonucleotide 5'-peptide-conjugates via copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition in aqueous solution is described. Synthesis of reagents was accomplished by on-column derivatization of corresponding peptides and oligonucleotides. This method is well suited for the preparation of peptide-oligonucleotide conjugates containing 1,2,3-triazole linkage between the 5'-position of an oligonucleotide and the N-terminus of a peptide.
authors.affiliation: a School of Material and Chemical Engineering, Henan Institute of Engineering , Zhengzhou , China.
authors.forename: Qunpeng
authors.initials: Q
authors.lastname: Duan
authors.forename: Kui
authors.initials: K
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Dongxin
authors.initials: D
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D057930:Click Chemistry / Q000379:methods; D006868:Hydrolysis; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D014230:Triazoles / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D010455:Peptides; D014230:Triazoles
keywords: Peptides; click chemistry; conjugates; oligonucleotides