title: The pathogenic potential of Pseudomonas fluorescens MFN1032 on enterocytes can be modulated by serotonin, substance P and epinephrine.
abstract: Pseudomonas fluorescens is a commensal bacterium present at low level in the human digestive tract that has also been reported in many clinical samples (blood, urinary tract, skin, lung, etc.) and sometimes associated with acute opportunistic infections. It has recently been found that the human β-defensin-2 can enhance the pathogenic potential of P. fluorescens. In this study, we evaluated the effect of other intestinal molecules (5HT, SP and Epi) on growth and virulence of the clinical strain P. fluorescens MFN1032. We found that P. fluorescens MFN1032 growth was not mainly affected by these factors, but several modifications in the virulence behavior of this bacterium were observed. 5HT, SP and Epi were able to modulate the motility of P. fluorescens MFN1032. 5HT and SP had an effect on pyoverdin production and IL-8 secretion, respectively. Infection of Caco-2/TC7 cells with P. fluorescens MFN1032 pretreated by SP or Epi enhanced the permeability of the monolayers and led to a partial delocalization of F-actin to the cytoplasm. These findings show that some intestinal molecules can modulate the pathogenic potential of P. fluorescens MFN1032. We can hypothesize that this dialogue between the host and the human gut microbiota may participate in health and disease.
authors.affiliation: Laboratoire de Microbiologie, Signaux et Microenvironnement (LMSM) EA4312, Université de Rouen, Évreux, France, kelly.biaggini@univ-rouen.fr.
authors.forename: Kelly
authors.initials: K
authors.lastname: Biaggini
authors.forename: Corinne
authors.initials: C
authors.lastname: Barbey
authors.forename: Valérie
authors.initials: V
authors.lastname: Borrel
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.forename: Pierre
authors.initials: P
authors.lastname: Déchelotte
authors.forename: Nathalie
authors.initials: N
authors.lastname: Connil
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018938:Caco-2 Cells; D020895:Enterocytes / Q000382:microbiology*; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000472:pathogenicity; D012701:Serotonin / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology*; D014774:Virulence / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D012701:Serotonin; D013373:Substance P; D004837:Epinephrine