title: Cordyceps sinensis attenuates renal fibrosis and suppresses BAG3 induction in obstructed rat kidney.
abstract: BAG3 regulates a number of cellular processes, including cell proliferation, apoptosis, adhesion and migration, and epithelial-mesenchymal transition (EMT). However, the role of BAG3 in renal tubular EMT and renal interstitial fibrosis remains elusive. This study aimed to examine the dynamic expression of BAG3 during renal fibrosis, and to investigate the efficacy of Cordyceps sinensis (C. sinensis) on renal fibrosis. A rat model of unilateral ureteral obstruction (UUO) was established, and the expression of BAG3 and α-SMA, and the efficacy of C. sinensis on renal fibrosis induced by UUO were examined. The results showed that UUO led to collagen accumulation, which was significantly suppressed by C. sinensis. UUO increased the expression of BAG3 and α-SMA, a mesenchymal marker, while UUO induced BAG3 and α-SMA expression was significantly inhibited by C. sinensis. In addition, immunohistochemical staining demonstrated that BAG3 immunoreactivity was restricted to tubular epithelium. In conclusion, BAG3 is a potential target for the prevention and/or treatment of renal fibrosis, and C. Sinensis is a promising agent for renal fibrosis.
authors.affiliation: Department of Nephrology, Shengjing Hospital, China Medical University Shenyang 110004, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Du
authors.affiliation: Department of Endocrinology & Metabolism, The 1st Affiliated Hospital, China Medical University Shenyang 110001, China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Endocrinology & Metabolism, The 1st Affiliated Hospital, China Medical University Shenyang 110001, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Geriatrics, The 1st Affiliated Hospital, China Medical University Shenyang 110001, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry & Molecular Biology, China Medical University Shenyang 110001, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Zong
authors.affiliation: Department of Endocrinology & Metabolism, The 1st Affiliated Hospital, China Medical University Shenyang 110001, China.
authors.forename: Zhen-Xian
authors.initials: ZX
authors.lastname: Du
authors.affiliation: Department of Nephrology, Shengjing Hospital, China Medical University Shenyang 110004, China.
authors.forename: De-Tian
authors.initials: DT
authors.lastname: Li
authors.affiliation: Department of Biochemistry & Molecular Biology, China Medical University Shenyang 110001, China.
authors.forename: Hua-Qin
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University Shenyang 110004, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University Shenyang 110004, China.
authors.forename: Jia-Ning
authors.initials: JN
authors.lastname: Miao
authors.affiliation: Department of Nephrology, Shengjing Hospital, China Medical University Shenyang 110004, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Bian
keywords: BAG3; Cordyceps sinensis; renal fibrosis; unilateral ureteral obstruction