title: Novel microsatellites for Calibrachoa heterophylla (Solanaceae) endemic to the South Atlantic Coastal Plain of South America.
abstract: OBJECTIVE
  Calibrachoa heterophylla (Solanaceae) is a petunia species restricted to the South Atlantic Coastal Plain of South America and presents a recent history of colonization from continental to coastal environments and diversification following the formation of the Coastal Plain during the Quaternary period.
  
  
  RESULTS
  This study reports a suite of 16 microsatellite loci for C. heterophylla. The applicability of these markers was assessed by genotyping 57 individuals from two natural populations. Of the 16 described loci, 12 were found to be polymorphic. Successful cross-amplification tests were obtained using 12 Calibrachoa species.
  
  
  CONCLUSIONS
  The development of microsatellite markers will be useful to recover the contemporary history of the colonization of the Coastal Plain and to provide information for the conservation of this endemic species.
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91501-970, Brazil.
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Silva-Arias
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91501-970, Brazil.
authors.forename: Geraldo
authors.initials: G
authors.lastname: Mäder
authors.affiliation: Laboratory of Genomic and Molecular Biology, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90610-001, Brazil.
authors.forename: Sandro L
authors.initials: SL
authors.lastname: Bonatto
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91501-970, Brazil.
authors.forename: Loreta B
authors.initials: LB
authors.lastname: Freitas
keywords: Calibrachoa heterophylla; Solanaceae; cross-amplification; population genetics; simple sequence repeat (SSR) markers; wild petunia