title: Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate.
abstract: Here we describe the preparation of a delivery vehicle for controlled release of Sonic hedgehog (Shh). The vehicle contains a synthetic polycation and heparin which interact by polyvalent charge attraction and rapidly self-assemble into liquid coacervate droplets. The coacervate loads Shh with high efficiency, protects its bioactivity, and provides sustained and localized release at the site of application. Shh coacervate may be injected directly or coated onto a polymeric scaffold for tissue engineering approaches, as described here.
authors.affiliation: Department of Bioengineering, University of Pittsburgh, 302 Benedum Hall, 3700 O'Hara St.,, Pittsburgh, PA, 15219, USA.
authors.forename: Noah Ray
authors.initials: NR
authors.lastname: Johnson
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D004337:Drug Carriers; D016503:Drug Delivery Systems; D065546:Drug Liberation; D053823:Hedgehog Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D053823:Hedgehog Proteins; D006493:Heparin; D011073:Polyamines; D000071228:Polyelectrolytes