title: Antifungal efficiency of a lipopeptide biosurfactant derived from Bacillus subtilis SPB1 versus the phytopathogenic fungus, Fusarium solani.
abstract: Bacillus subtilis SPB1 lipopeptides were evaluated as a natural antifungal agent against Fusarium solani infestation. In vitro antifungal assay showed a minimal inhibitory concentration of about 3 mg/ml with a fungicidal mode of action. In fact, treatment of F. solani by SPB1 lipopeptides generated excessive lyses of the mycelium and caused polynucleation and destruction of the related spores together with a total inhibition of spore production. Furthermore, an inhibition of germination potency accompanied with a high spore blowing was observed. Moreover, in order to be applied in agricultural field, in vivo antifungal activity was proved against the dry rot potato tubers caused by F. solani. Preventive treatment appeared as the most promising as after 20 days of fungi inoculation, rot invasion was reduced by almost 78%, in comparison to that of non-treated one. When treating infected tomato plants, disease symptoms were reduced by almost 100% when applying the curative method. Results of this study are very promising as it enables the use of the crude lipopeptide preparation of B. subtilis SPB1 as a potent natural fungicide that could effectively control the infection of F. solani in tomato and potato tubers at a concentration similar to the commercial fungicide hymexazol and therefore prevent the damage of olive tree.
authors.affiliation: Unit "Enzymes and Bioconversion", National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia. inesmnif2011@gmail.com.|Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia. inesmnif2011@gmail.com.
authors.forename: Ines
authors.initials: I
authors.lastname: Mnif
authors.affiliation: Higher School of Agriculture of Kef, 7119, Kef, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Hammami
authors.affiliation: Laboratory "Amélioration et Protection des Ressources Génétiques de l'Olivier", Sfax BP1087, Institut of Olivier, IRESA - University of Sfax, Sfax, Tunisia.
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Triki
authors.affiliation: Laboratory "Amélioration et Protection des Ressources Génétiques de l'Olivier", Sfax BP1087, Institut of Olivier, IRESA - University of Sfax, Sfax, Tunisia.
authors.forename: Manel Cheffi
authors.initials: MC
authors.lastname: Azabou
authors.affiliation: Unit "Enzymes and Bioconversion", National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouze-Chaabouni
authors.affiliation: Unit "Enzymes and Bioconversion", National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia. dhouhag@yahoo.fr.|Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia. dhouhag@yahoo.fr.
authors.forename: Dhouha
authors.initials: D
authors.lastname: Ghribi
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000737:chemistry* / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects*; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035281:Plant Tubers / Q000382:microbiology; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D055666:Lipopeptides; D013501:Surface-Active Agents
keywords: Antifungal activity; Bio-control; F. solani; Potato tuber rot; SPB1 lipopeptide; Tomato root rot