title: Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155.
abstract: MicroRNA-155 plays an important role in the inflammatory response macrophages, while present studies identified that miR-155 was up-expressed in gastritis induced by Helicobacter pylori. We found that miR-155 was over expressed in macrophages infected with H. pylori in vivo or in vitro. Subsequently, inflammatory cytokines IL-23, IL-10, TNF-α and IL-8 were increased significantly, and the expression of CD80, CD86 or COX2, NOS2. Were enhanced in H. pylori infection macrophages by regulated with miR-155mimics. Furthermore, the apoptosis of macrophages induced by H. pylori was increased obviously due to the over-expression of miR-155. Therefore, these observations indicated that miR-155 may act as a inflammatory promoter in H. pylori infected macrophages. These findings contribute us to understand the functions of miR-155 in gastritis induced by H. pylori furtherly.
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Chongqing Cancer Institute Chongqing 400030, People's Republic of China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014162:Transfection; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; C524942:MIRN155 microRNA, human; D035683:MicroRNAs
keywords: Helicobacter pylori; Macrophage; miR-155