title: Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure.
abstract: The aim of the study was to isolate and characterize the lactic acid bacteria (LAB) from animal manure. Among the thirty LAB strains, four strains, namely, KCC-25, KCC-26, KCC-27, and KCC-28, showed good cell growth and antifungal activity and were selected for further characterization. Biochemical and physiology properties of strains confirmed that the strains are related to the Lactobacillus sp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towards Lactobacillus plantarum. The strains exhibited susceptibility against commonly used antibiotics with negative hemolytic property. Strains KCC-25, KCC-26, KCC-27, and KCC-28 showed strong antifungal activity against Aspergillus fumigatus, Penicillium chrysogenum, Penicillium roqueforti, Botrytis elliptica, and Fusarium oxysporum, respectively. Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids. Further, the production of extracellular proteolytic and glycolytic enzymes, survival under low pH, bile salts, and gastric juice together with positive bile salt hydrolase (Bsh) activity, cholesterol lowering, cell surface hydrophobicity, and aggregation properties were the strains advantages. Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract. In conclusion, novel L. plantarum KCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.
authors.forename: Soundharrajan
authors.initials: S
authors.lastname: Ilavenil
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Park
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.
authors.forename: Mayakrishnan
authors.initials: M
authors.lastname: Vijayakumar
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Mariadhas Valan
authors.initials: MV
authors.lastname: Arasu
authors.affiliation: The United Graduate School of Agricultural Sciences, Tottori University, Tottori 680-8553, Japan.
authors.forename: Da Hye
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, PRIST University, Thanjavur, Tamil Nadu 613403, India.
authors.forename: Sivanesan
authors.initials: S
authors.lastname: Ravikumar
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.
authors.forename: Ki Choon
authors.initials: KC
authors.lastname: Choi
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001647:Bile Acids and Salts / Q000494:pharmacology; D005285:Fermentation; D005658:Fungi / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D008372:Manure / Q000382:microbiology*; D055442:Metabolome; D008826:Microbial Sensitivity Tests; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D008372:Manure; D012336:RNA, Ribosomal, 16S