title: Inhibition of tobacco smoke-induced bladder MAPK activation and epithelial-mesenchymal transition in mice by curcumin.
abstract: Tobacco smoke (TS) has been shown to cause bladder cancer. Epithelial-mesenchymal transition (EMT) is a crucial pathophysiological process in cancer development. MAPK pathways play central roles in tumorigenesis including EMT process. Curcumin is a promising chemopreventive agent for several types of cancers. In the present study we investigated the effects of TS on MAPK pathway activation and EMT alterations in the bladder of mice, and the preventive effect of curcumin was further examined. Results showed that exposure of mice to TS for 12 weeks resulted in activation of ERK1/2, JNK, p38 and ERK5 MAPK pathways as well as AP-1 proteins in bladder. TS reduced mRNA and protein expression levels of epithelial markers E-cadherin and ZO-1, while mRNA and protein expression levels of the mesenchymal markers vimentin and N-cadherin were increased. Curcumin treatment effectively attenuated TS-triggered activation of ERK1/2, JNK and p38 MAPK pathways, AP-1 proteins and EMT alterations in bladder tissue. These results suggest the protective effects of curcumin in TS-induced MAPK activation and EMT, thus providing new insights into the chemoprevention of TS-associated bladder cancer.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Institute of Food Safety and Assessment, Jiangsu Provincial Center for Disease Control and Prevention Nanjing 211166, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Geng
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Xie
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Jieshu
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Geng
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University Nanjing 211166, China ; The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University Nanjing 211166, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Department of Clinical Nutrition, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China Guangzhou 510060, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Han
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D004789:Enzyme Activation; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D001743:Urinary Bladder / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*
chemical_list: D016588:Anticarcinogenic Agents; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D014028:Tobacco Smoke Pollution; D020928:Mitogen-Activated Protein Kinases; D003474:Curcumin
keywords: MAPK pathways; Tobacco smoke; bladder cancer; curcumin; epithelial-mesenchymal transition