title: Multi-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS system with timed and highly selective reaction monitoring.
abstract: Mycotoxins have the potential to enter the human food chain through carry-over of contaminants from feed into animal-derived products. The objective of the study was to develop a reliable and sensitive method for the analysis of 30 mycotoxins in animal feed and animal-derived food (meat, edible animal tissues, and milk) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the study, three extraction procedures, as well as various cleanup procedures, were evaluated to select the most suitable sample preparation procedure for different sample matrices. In addition, timed and highly selective reaction monitoring on LC-MS/MS was used to filter out isobaric matrix interferences. The performance characteristics (linearity, sensitivity, recovery, precision, and specificity) of the method were determined according to Commission Decision 2002/657/EC and 401/2006/EC. The established method was successfully applied to screening of mycotoxins in animal feed and animal-derived food. The results indicated that mycotoxin contamination in feed directly influenced the presence of mycotoxin in animal-derived food. Graphical abstract Multi-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu, 210095, China.|Institute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, 201403, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, 201403, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu, 210095, China.
authors.forename: Lingchen
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu, 210095, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Institute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, 201403, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu, 210095, China.
authors.forename: Suquan
authors.initials: S
authors.lastname: Song
authors.affiliation: Institute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, 201403, China.
authors.forename: Shanhai
authors.initials: S
authors.lastname: Lin
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Taiyuan Road, Shanghai, 200031, China.
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu, 210095, China. zhouzl@njau.edu.cn.
authors.forename: Zhenlei
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu, 210095, China. jfhou@njau.edu.cn.
authors.forename: Jiafa
authors.initials: J
authors.lastname: Hou
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D011200:Poultry; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009183:Mycotoxins
keywords: Animal feed; Animal-derived food; High throughput; LC–MS/MS; Mycotoxin