title: Use of suboptimal sperm increases the risk of aneuploidy of the sex chromosomes in preimplantation blastocyst embryos.
abstract: OBJECTIVE
  To compare autosomal and sex chromosome aneuploidy rates of embryos derived from sperm with abnormal and normal parameters.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  Assisted reproduction center.
  
  
  METHODS
  Three thousand eight hundred thirty-five embryos generated from 629 couples undergoing IVF.
  
  
  METHODS
  None.
  
  
  METHODS
  Incidence of aneuploidy in the trophectoderm of blastocyst embryos derived from standard IVF embryos and intracytoplasmic (ICSI) males with normal and oligozoospermic semen samples, in couples with donor eggs (mean maternal age, 25.0 years) and their own eggs (mean maternal age, 35.4 years).
  
  
  RESULTS
  The rate of sex chromosome aneuploidy was significantly (around threefold) higher in the oligozoospermic group compared with in both control groups (standard vs. ICSI insemination). This applied whether donor (young) or own (older) eggs were used. Significant differences were seen in the oligozoospermic samples for autosomes 1, 2, 11 (own eggs), and 18 (donor eggs) compared with both control groups; however, no significant difference was seen between each of the treatment groups for the overall rate of autosomal aneuploidy. No significant differences were seen between the two control groups (normozoospermic males, standard vs. ICSI insemination) in either of the egg group types for any chromosome pairs.
  
  
  CONCLUSIONS
  Severe male factor infertility is associated with a significant increase in the occurrence of sex chromosome abnormalities in blastocyst embryos compared with in embryos derived from normal semen samples. Aneuploidy rates in embryos derived from sperm with normal parameters were not significantly different whether ICSI or standard insemination was used to achieve fertilization. These results highlight severe male factor infertility as a possible referral category for preimplantation comprehensive chromosomal screening.
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon; School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom.
authors.forename: Alison
authors.initials: A
authors.lastname: Coates
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon.
authors.forename: John S
authors.initials: JS
authors.lastname: Hesla
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon.
authors.forename: Amanda
authors.initials: A
authors.lastname: Hurliman
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon.
authors.forename: Breanne
authors.initials: B
authors.lastname: Coate
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Holmes
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Matthews
authors.affiliation: Oregon Reproductive Medicine, Portland, Oregon.
authors.forename: Emily L
authors.initials: EL
authors.lastname: Mounts
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom.
authors.forename: Kara J
authors.initials: KJ
authors.lastname: Turner
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom; Illumina, Cambridge, United Kingdom.
authors.forename: Alan R
authors.initials: AR
authors.lastname: Thornhill
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom. Electronic address: d.k.griffin@kent.ac.uk.
authors.forename: Darren K
authors.initials: DK
authors.lastname: Griffin
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001755:Blastocyst / Q000378:metabolism / Q000473:pathology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies; D012307:Risk Factors; D055101:Semen Analysis / Q000009:adverse effects*; D012729:Sex Chromosome Aberrations / Q000196:embryology / Q000706:statistics & numerical data; D013094:Spermatozoa / Q000473:pathology
keywords: ICSI; Sex chromosome; aneuploidy; autosome