title: Deconvolution method for specific and nonspecific binding of ligand to multiprotein complex by native mass spectrometry.
abstract: In native mass spectrometry, it has been difficult to discriminate between specific bindings of a ligand to a multiprotein complex target from the nonspecific interactions. Here, we present a deconvolution model that consists of two levels of data reduction. At the first level, the apparent association binding constants are extracted from the measured intensities of the target/ligand complexes by varying ligand concentration. At the second level, two functional forms representing the specific and nonspecific binding events are fit to the apparent binding constants obtained from the first level of modeling. Using this approach, we found that a power-law distribution described nonspecific binding of α-amanitin to yeast RNA polymerase II. Moreover, treating the concentration of the multiprotein complex as a fitting parameter reduced the impact of inaccuracies in this experimental measurement on the apparent association constants. This model improves upon current methods for separating specific and nonspecific binding to large, multiprotein complexes in native mass spectrometry, by modeling nonspecific binding with a power-law function.
authors.affiliation: †Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158-2517, United States.|‡Institute for Neurodegenerative Diseases and Department of Neurology, University of California, San Francisco, California 94143-0518, United States.
authors.forename: Shenheng
authors.initials: S
authors.lastname: Guan
authors.affiliation: †Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158-2517, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Trnka
authors.affiliation: §Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, United States.
authors.forename: David A
authors.initials: DA
authors.lastname: Bushnell
authors.affiliation: §Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, United States.
authors.forename: Philip J J
authors.initials: PJ
authors.lastname: Robinson
authors.affiliation: †Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158-2517, United States.|‡Institute for Neurodegenerative Diseases and Department of Neurology, University of California, San Francisco, California 94143-0518, United States.
authors.forename: Jason E
authors.initials: JE
authors.lastname: Gestwicki
authors.affiliation: †Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158-2517, United States.
authors.forename: Alma L
authors.initials: AL
authors.lastname: Burlingame
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry / Q000378:metabolism; D053959:Alpha-Amanitin / Q000737:chemistry* / Q000378:metabolism; D003402:Creatine Kinase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D011485:Protein Binding; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D020123:Sirolimus / Q000737:chemistry / Q000378:metabolism; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D053959:Alpha-Amanitin; D008024:Ligands; D011994:Recombinant Proteins; D000244:Adenosine Diphosphate; D003402:Creatine Kinase; D012319:RNA Polymerase II; D022061:Tacrolimus Binding Protein 1A; D020123:Sirolimus