title: The role of EphB4 and IGF-IR expression in breast cancer cells.
abstract: OBJECTIVE
  to investigate the role of EphB4 and IGF-1R in the proliferation and migration of breast cancer.
  
  
  METHODS
  The relative mRNA levels of EphB4 were measured by RT-PCR. The proliferation of the cells was determined by MTT assay, and cells migration and invasive ability was analyzed using the scratch migration assay.
  
  
  RESULTS
  The expression of EphB4 in control group was significantly decreased when compared with IGF-I group (P<0.001). The expression of EphB4 in IGF-I+LY and LY group were lower than that of the control group (P<0.001).The cell proliferation and migration ability of the cells in IGF-I group increased significantly compared to the cells in the control group (P<0.001), while the cells in IGF-1+LY group and LY group showed a decreased proliferation and migration ability compared to the control group (P<0.001).
  
  
  CONCLUSIONS
  IGF-IR might be a upstream gene of EphB4. Besides, higher expression of EphB4 shows increased tumor proliferation and migration in breast cells. The study of EphB4 upstream gene and signaling pathway can provide more targeted anti-tumor point selection for targeted therapy.
authors.affiliation: Department of Seven-year Clinical Medicine, Research Institute of Integrated Traditional and Western Medicine of Dalian Medical University Dalian 116044, China.
authors.forename: Gena
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Oncology, Second Hospital of Dalian Medical University Dalian 116023, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002465:Cell Movement; D002867:Chromones / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D061986:MCF-7 Cells; D008875:Middle Aged; D009025:Morpholines / Q000494:pharmacology; D009361:Neoplasm Invasiveness; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D036224:Receptor, EphB4 / Q000235:genetics / Q000378:metabolism*; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D002867:Chromones; C000597236:IGF1R protein, human; D009025:Morpholines; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D017451:Receptors, Somatomedin; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D007334:Insulin-Like Growth Factor I; D036224:Receptor, EphB4; D017526:Receptor, IGF Type 1
keywords: EphB4; IGF-IR; breast cancer cell