title: Light- and pH-activated intracellular drug release from polymeric mesoporous silica nanoparticles.
abstract: Surface modified mesoporous silica nanoparticles (MSNs) with reduced toxicity were prepared for light and pH dual triggerable drug delivery system. Both 413 nm light and acidic environment can activate the drug release process, improving the pharmacological action. By applying rhodamine B (RhB) as a model drug, the accumulative RhB release is as high as 95% in pH 5.0 and in irradiation of 413 nm light, compared to only 55% in pH 7.4 and in dark. The anti-cancer drug camptothecin (CPT) loaded nanoparticles can kill cancer cells with IC₅₀ value of 0.02 μg mL(-1) in exposure of 413 nm light, which is much lower than free CPT (about 0.1 μg mL(-1)). Multimodal nonlinear optical imaging microscopy (NLOM) was employed to acquire in vitro coherent anti-Stokes Raman (CARS) and two-photon excited fluorescence (TPEF) images of live MCF-7 cells and showed that the nanoparticles can be taken up by breast tumor cell MCF-7 with high efficiency, indicating its great potential for anti-cancer drug delivery system.
authors.affiliation: Department of Mechanical, University of Manitoba, Winnipeg, Manitoba, Canada; Manitoba Institute of Child Health, Winnipeg, Manitoba, Canada; Key Laboratory of Photochemical Conversion and Optoelectronic, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns, and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns, and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, Third Military Medical University, Chongqing 400038, China. Electronic address: junwupro@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Alex C-T
authors.initials: AC
authors.lastname: Ko
authors.affiliation: Department of Mechanical, University of Manitoba, Winnipeg, Manitoba, Canada; Manitoba Institute of Child Health, Winnipeg, Manitoba, Canada; National Research Council Canada, Medical Device Portfolio, Winnipeg, Manitoba, Canada. Electronic address: malcolm.xing@umanitoba.ca.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D061986:MCF-7 Cells; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D012822:Silicon Dioxide
keywords: Anticancer drug release; Light sensitive; Live cell multimodal nonlinear optical imaging (CARS and TPEF); Mesoporous silica nanoparticle; pH sensitive