title: Anticancer activity of Kalanchoe tubiflora extract against human lung cancer cells in vitro and in vivo.
abstract: Uncontrolled cell proliferation is a common feature of human cancer. Some of herbal extract or plant-derived medicine had been shown as an important source of effective anticancer agents. We previously reported that an n-BuOH-soluble fraction of Kalanchoe tubiflora has antiproliferative activity by inducing mitotic catastrophe. In this study, we showed that the H2O-soluble fraction of Kalanchoe tubiflora (KT-W) caused cell cycle arrest, and senescence-inducing activities in A549 cells. We used 2 dimensional PAGE to analyze the protein expression levels after KT-W treatment, and identified that the energy metabolism-related proteins and senescence-related proteins were disturbed. In vivo experiments showed that the tumor growths in A549-xenografted nude mice were effectively inhibited by KT-W. Our findings implied that KT-W is a putative antitumor agent by inducing cell cycle arrest and senescence. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1663-1673, 2016.
authors.affiliation: Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan.|Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan.
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Hsieh
authors.affiliation: Department of Medical Research, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan.
authors.forename: Hsuan-Shun
authors.initials: HS
authors.lastname: Huang
authors.affiliation: Graduate institute of Natural Products, Healthy Aging Research Center College of Medicine, Chang Gung University, Taoyuan, Taiwan.|Chinese Herbal Medicine Research Team, Healthy Aging Research Center College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Yann-Lii
authors.initials: YL
authors.lastname: Leu
authors.affiliation: Institute of Biotechnology, National Dong-Hwa University, Hualien, Taiwan.
authors.forename: Kou-Cheng
authors.initials: KC
authors.lastname: Peng
authors.affiliation: Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.
authors.forename: Chih-Jui
authors.initials: CJ
authors.lastname: Chang
authors.affiliation: Department of Medical Research, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan. mchang@mail.tcu.edu.tw.|Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan. mchang@mail.tcu.edu.tw.
authors.forename: Meng-Ya
authors.initials: MY
authors.lastname: Chang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D031962:Kalanchoe; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
keywords: A549 xenograft model; H2O-soluble fraction; Kalanchoe tubiflora; cell cycle arrest; lung cancer; senescence; tumor growth inhibition