title: Complex wireframe DNA origami nanostructures with multi-arm junction vertices.
abstract: Structural DNA nanotechnology and the DNA origami technique, in particular, have provided a range of spatially addressable two- and three-dimensional nanostructures. These structures are, however, typically formed of tightly packed parallel helices. The development of wireframe structures should allow the creation of novel designs with unique functionalities, but engineering complex wireframe architectures with arbitrarily designed connections between selected vertices in three-dimensional space remains a challenge. Here, we report a design strategy for fabricating finite-size wireframe DNA nanostructures with high complexity and programmability. In our approach, the vertices are represented by n × 4 multi-arm junctions (n = 2-10) with controlled angles, and the lines are represented by antiparallel DNA crossover tiles of variable lengths. Scaffold strands are used to integrate the vertices and lines into fully assembled structures displaying intricate architectures. To demonstrate the versatility of the technique, a series of two-dimensional designs including quasi-crystalline patterns and curvilinear arrays or variable curvatures, and three-dimensional designs including a complex snub cube and a reconfigurable Archimedean solid were constructed.
authors.affiliation: Center for Molecular Design and Biomimetics, The Biodesign Institute and the Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Center for Molecular Design and Biomimetics, The Biodesign Institute and the Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Shuoxing
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Siyu
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Chengde
authors.initials: C
authors.lastname: Mao
authors.affiliation: Center for Molecular Design and Biomimetics, The Biodesign Institute and the Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Center for Molecular Design and Biomimetics, The Biodesign Institute and the Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D004247:DNA / Q000737:chemistry / Q000648:ultrastructure*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D010316:Particle Size
chemical_list: D004247:DNA