title: Giant Acceleration of Diffusion Observed in a Single-Molecule Experiment on F(1)-ATPase.
abstract: The giant acceleration (GA) of diffusion is a universal phenomenon predicted by the theoretical analysis given by Reimann et al. [Phys. Rev. Lett. 87, 010602 (2001)]. Here we apply the theory of the GA of diffusion to a single-molecule experiment on a rotary motor protein, F(1), which is a component of F(o)F(1) adenosine triphosphate synthase. We discuss the energetic properties of F(1) and identify a high energy barrier of the rotary potential to be 20k(B)T, with the condition that the adenosine diphosphates are tightly bound to the F(1) catalytic sites. To conclude, the GA of diffusion is useful for measuring energy barriers in nonequilibrium and single-molecule experiments.
authors.affiliation: Department of Applied Physics, School of Engineering, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Ryunosuke
authors.initials: R
authors.lastname: Hayashi
authors.affiliation: Department of Applied Physics, School of Engineering, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Department of Applied Physics, School of Engineering, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Department of Applied Physics, School of Engineering, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Kudo
authors.affiliation: Institute of Multidisciplinary Research for Advanced Material, Tohoku University, Sendai 980-8577, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Department of Applied Chemistry, School of Engineering, the University of Tokyo, Tokyo 113-8654, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Noji
authors.affiliation: Department of Applied Physics, School of Engineering, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D004058:Diffusion; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D006180:Proton-Translocating ATPases