title: Subdiffraction resolution microscopy methods for analyzing centrosomes organization.
abstract: In this chapter, we describe the current methods of examining the structure of centrosomes by fluorescence subdiffraction microscopy. By using recently developed microscopy techniques, centrosomal proteins can now be examined in cells with a resolution of only a few nanometers, a level of molecular detail beyond the reach of traditional cell biology methods as confocal and widefield microscopy. We emphasize imaging by three-dimensional structured illumination microscopy, stochastic optical reconstruction microscopy, and quantitative approaches to image data analysis.
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, ON, Canada; Cell Biology Program, The Hospital for Sick Children, Toronto, ON, Canada; Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
authors.forename: Vito
authors.initials: V
authors.lastname: Mennella
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, ON, Canada; Cell Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.
authors.forename: Rachel
authors.initials: R
authors.lastname: Hanna
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, ON, Canada; Cell Biology Program, The Hospital for Sick Children, Toronto, ON, Canada.
authors.forename: Moshe
authors.initials: M
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D018385:Centrosome / Q000502:physiology / Q000648:ultrastructure*; D004331:Drosophila melanogaster; D019084:Fluorescent Antibody Technique, Indirect; D008856:Microscopy, Fluorescence / Q000379:methods
keywords: 3DSIM; Averaging; Centrosome structure; Fluorescence light microscopy; Macromolecular assembly; PCM; STORM; Subdiffraction imaging; Superresolution imaging