title: Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis.
abstract: Sirtuins have been implicated in diverse biological processes, including oxidative stress, energy metabolism, cell migration, and aging. Here, we employed Sirtuin inhibitors, nicotinamide (NAM) and Sirtinol, to investigate their effects on porcine oocyte maturation respectively. The rate of polar body extrusion in porcine oocytes decreased after treatment with NAM and Sirtinol, accompanied with the failure of cumulus cell expansion. We further found that NAM and Sirtinol significantly disrupted oocyte polarity, and inhibited the formation of actin cap and cortical granule-free domain (CGFD). Moreover, the abnormal spindles and misaligned chromosomes were readily detected during porcine oocyte maturation after treatment with NAM and Sirtinol. Together, these results suggest that Sirtuins are involved in cortical polarity and spindle organization in porcine oocytes.
authors.affiliation: College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Rujun
authors.initials: R
authors.lastname: Ma
authors.affiliation: College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Ding
authors.affiliation: College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yinxue
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D016764:Cell Polarity; D033481:Chromosomes, Mammalian / Q000187:drug effects; D054885:Cumulus Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D008540:Meiosis / Q000187:drug effects*; D009284:Naphthols / Q000494:pharmacology*; D009536:Niacinamide / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D059705:Polar Bodies / Q000187:drug effects; D061251:Primary Cell Culture; D037761:Sirtuins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects / Q000648:ultrastructure; D013552:Swine
chemical_list: D000199:Actins; D001549:Benzamides; D056572:Histone Deacetylase Inhibitors; D009284:Naphthols; C439060:sirtinol; D009536:Niacinamide; D037761:Sirtuins