title: [MICROBIAL COMMUNITIES ON KIDNEY STONES].
abstract: The clinical material obtained surgically in patients with kidney stone disease (KSD) was tested for content of the stone microflora using PCR and standard microbiological methods. It was demonstrated that about 50% of stones in patients with KSD were infected with various infection agents as observed using standard microbiological and molecular genetic methods. The percentage of detection of the Mycoplasma hominis using cultural method is lower than the percentage detected using PCR, which is due to difficult isolation and cultivation, as well as DNA fragments of mycoplasma observed after antibiotic therapy. Studies based on modern microscopy methods showed that microorganisms on the surface of the kidney stone formed multispecies biofilms.
authors.forename: Yu M
authors.initials: YM
authors.lastname: Romanova
authors.forename: N S
authors.initials: NS
authors.lastname: Mulabaev
authors.forename: E R
authors.initials: ER
authors.lastname: Tolordava
authors.forename: A V
authors.initials: AV
authors.lastname: Seregin
authors.forename: I V
authors.initials: IV
authors.lastname: Seregin
authors.forename: N V
authors.initials: NV
authors.lastname: Alexeeva
authors.forename: T V
authors.initials: TV
authors.lastname: Stepanova
authors.forename: G A
authors.initials: GA
authors.lastname: Levina
authors.forename: O I
authors.initials: OI
authors.lastname: Barhatova
authors.forename: N A
authors.initials: NA
authors.lastname: Gamova
authors.forename: S A
authors.initials: SA
authors.lastname: Goncharova
authors.forename: L V
authors.initials: LV
authors.lastname: Didenko
authors.forename: I V
authors.initials: IV
authors.lastname: Rakovskaya
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects; D006801:Humans; D007669:Kidney Calculi / Q000382:microbiology* / Q000601:surgery; D059013:Microbial Consortia / Q000502:physiology; D008854:Microscopy, Electron; D019535:Mycoplasma hominis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D016133:Polymerase Chain Reaction; D014509:Ureaplasma / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents