title: Protective effect of Blumea lacera DC aerial parts in indomethacin-induced enterocolitis in rats.
abstract: Traditionally Blumea lacera DC is used to treat inflammation and bowel ailments. Lack of specific, curative treatment for IBD enticed us to investigate the therapeutic efficacy of ethanolic extract of aerial parts of Blumea lacera DC (EEBL) against indomethacin-induced enterocolitis. Male Wistar rats were divided into six groups (n = 5) and different doses of EEBL (100 and 200 mg/kg, p.o) and sulphasalazine (100 mg/kg, p.o) were administered for seven days. Enterocolitis was induced by two subsequent doses of indomethacin (7.5 mg/kg, s.c) on 7th and 8th day. Treatments were continued up to 12th day and sacrificed. The protective effect was assessed on the basis of macroscopic scores of ileum strips, changes in biochemical parameters such as serum lactate dehydrogenase (LDH), tissue myeloperoxidase (MPO), lipid peroxidation (LPO), and total thiols (TT). Further, activity was ascertained by histopathological evaluations. HPLC fingerprinting profiling of EEBL was also carried out. Pre-treatment with EEBL or sulphasalazine significantly attenuated the indomethacin-induced proximal ileal damage, elevated levels of serum LDH, tissue MPO, LPO and lower levels of TT. Further, observed activity of EEBL was well correlated with histopathological alterations. The results revealed the protective action of the title plant against the indomethacin-induced enterocolitis in rats, which might be attributed by its antioxidant, anti-inflammatory, antimicrobial, and membrane-stabilizing properties.
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India. bsantosh014@gmail.com.
authors.forename: Santosh
authors.initials: S
authors.lastname: Basnet
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India. adh.ashish123@gmail.com.
authors.forename: Ashish
authors.initials: A
authors.lastname: Adhikari
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India. munnuviji@gmail.com.
authors.forename: Vijaya Kumar
authors.initials: VK
authors.lastname: Sachidananda
authors.affiliation: Department of Biomedical Science, College of Pharmacy, Shaqra University, Al-Dawadmi, Kingdom of Saudi Arabia. t_swamy@hotmail.com.
authors.forename: Boreddy Shivanandappa
authors.initials: BS
authors.lastname: Thippeswamy
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India. veeresh36@gmail.com.
authors.forename: Veeresh Prabhakar
authors.initials: VP
authors.lastname: Veerapur
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D019659:Asteraceae / Q000737:chemistry*; D004760:Enterocolitis / Q000139:chemically induced* / Q000188:drug therapy*; D007213:Indomethacin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008517:Phytotherapy / Q000379:methods; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D007213:Indomethacin
keywords: Antioxidant; Blumea lacera; Crohn’s disease; HPLC; Inflammation; Inflammatory bowel disease