title: Identification by hydrophilic interaction and reversed-phase liquid chromatography-tandem mass spectrometry of peptides with antioxidant capacity in food residues.
abstract: HILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides.
authors.affiliation: Department of Analytical Chemistry, University of Alcalá. Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
authors.forename: Romy
authors.initials: R
authors.lastname: Vásquez-Villanueva
authors.affiliation: Department of Analytical Chemistry, University of Alcalá. Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Marina
authors.affiliation: Department of Analytical Chemistry, University of Alcalá. Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. Electronic address: concepcion.garcia@uah.es.
authors.forename: M Concepción
authors.initials: MC
authors.lastname: García
mesh_terms: D000975:Antioxidants / Q000032:analysis; D056148:Chromatography, Reverse-Phase; D005504:Food Analysis / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013820:Thermolysin / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010455:Peptides; D013820:Thermolysin
keywords: Antioxidant; Hydrophilic interaction; Liquid chromatography–tandem mass spectrometry; Peach seed; Peptides; Reversed-phase