title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge.
abstract: Vibrio splendidus is identified as one of the major pathogenic factors for the skin ulceration syndrome in sea cucumber (Apostichopus japonicus), which has vastly limited the development of the sea cucumber culture industry. In order to screen the immune genes involving Vibrio splendidus challenge in sea cucumber and explore the molecular mechanism of this process, the related transcriptome and gene expression profiling of resistant and susceptible biotypes of sea cucumber with Vibrio splendidus challenge were collected for analysis. A total of 319,455,942 trimmed reads were obtained, which were assembled into 186,658 contigs. After that, 89,891 representative contigs (without isoform) were clustered. The analysis of the gene expression profiling identified 358 differentially expression genes (DEGs) in the bacterial-resistant group, and 102 DEGs in the bacterial-susceptible group, compared with that in control group. According to the reported references and annotation information from BLAST, GO and KEGG, 30 putative bacterial-resistant genes and 19 putative bacterial-susceptible genes were identified from DEGs. The qRT-PCR results were consistent with the RNA-Seq results. Furthermore, many DGEs were involved in immune signaling related pathways, such as Endocytosis, Lysosome, MAPK, Chemokine and the ERBB signaling pathway.
authors.affiliation: Fisheries College, Ocean University of China, Qingdao 266100, China. gaohaijun1989@163.com.|Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. gaohaijun1989@163.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. liaomj@ysfri.ac.cn.
authors.forename: Meijie
authors.initials: M
authors.lastname: Liao
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. wangyg@ysfri.ac.cn.
authors.forename: Yingeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. libin@ysfri.ac.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. zhangzheng@ysfri.ac.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. rongxj@ysfri.ac.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Rong
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. chengp@ysfri.ac.cn.
authors.forename: Guiping
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. wanglan_0829@163.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D060467:Disease Resistance / Q000235:genetics; D056426:Genetic Loci; D020935:MAP Kinase Signaling System; D020641:Polymorphism, Single Nucleotide; D012616:Sea Cucumbers / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D059467:Transcriptome; D014733:Vibrio / Q000472:pathogenicity*
keywords: Vibrio splendidus; bacteria-resistant gene; bacteria-susceptible gene; differentially expressed genes; sea cucumber (Apostichopus japonicus); transcriptome sequencing