title: Finding of thiosulfate pathway for synthesis of organic sulfur compounds in Saccharomyces cerevisiae and improvement of ethanol production.
abstract: We found that Saccharomyces cerevisiae utilizes thiosulfate as a sole sulfur source. The energetically-favored thiosulfate rather than sulfate as sulfur sources is also more effective for improving growth and ethanol-production rate in S. cerevisiae due to high levels of intracellular NADPH during thiosulfate utilization.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Funahashi
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Kyohei
authors.initials: K
authors.lastname: Saiki
authors.affiliation: Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
authors.forename: Kurara
authors.initials: K
authors.lastname: Honda
authors.affiliation: Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sugiura
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawano
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan. Electronic address: iohtsu@bs.naist.jp.
authors.forename: Iwao
authors.initials: I
authors.lastname: Ohtsu
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Watanabe
authors.affiliation: Technical Research Laboratories, Kyowa Hakko Bio Co. Ltd., 1-1 Kyowa-cho, Hofu, Yamaguchi 747-8522, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Wakabayashi
authors.affiliation: Technical Research Laboratories, Kyowa Hakko Bio Co. Ltd., 1-1 Kyowa-cho, Hofu, Yamaguchi 747-8522, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Abe
authors.affiliation: MS Business Unit, Shimadzu Co., 1 Nishinokyo, Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakanishi
authors.affiliation: Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
mesh_terms: D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
chemical_list: D013885:Thiosulfates; D000431:Ethanol; D009249:NADP
keywords: Ethanol production; Pentose phoshate pathway; Saccharomyces cerevisiae; Sulfur metabolism; Sulfur metabolome; Thiosulfate assimilation