title: Upregulation of H19 promotes invasion and induces epithelial-to-mesenchymal transition in esophageal cancer.
abstract: Long non-coding RNAs (lncRNAs) have previously been reported to be involved in cancer invasion, proliferation and apoptosis. However, the association between the lncRNA, H19, and esophageal cancer (EC) has remained elusive. In the present study, reverse transcription quantitative-polymerase chain reaction revealed that the expression of H19 was significantly increased and associated with tumor depth and metastasis in 133 EC samples. Furthermore, MTT and Transwell assays revealed that overexpression of H19in vitropromoted the proliferation and invasion of EC cell lines, whereas knockdown of H19 inhibited the proliferation and invasion of EC cell lines. In addition, it was identified that an upregulation of H19 induced epithelial-to-mesenchymal transition, while the opposite effect was observed following the downregulation of H19. In conclusion, H19 has a significant role in the development of EC and may serve as a potential prognostic marker and therapeutic target for EC.
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Dermatology, Yancheng City No. 1 People's Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Thoracic Surgery, Yancheng City No. 1 People's Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Linwei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Pathology, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Yingting
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Weiyong
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Koulan
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Pathology, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China.
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Lv
keywords: H19; epithelial-to-mesenchymal transition; esophageal cancer; invasion; proliferation