title: Transformation of Shewanella baltica with ColE1-like and P1 plasmids and their maintenance during bacterial growth in cultures.
abstract: The presence of natural plasmids has been reported for many Shewanella isolates. However, knowledge about plasmid replication origin and segregation mechanisms is not extensive for this genus. Shewanella baltica is an important species in the marine environment due to its denitrification ability in oxygen-deficient zones and the potential role in bioremediation processes. However, no information about possible use of plasmid vectors in this species has been reported to date. Here we report that plasmids with ColE1-type and plasmid P1 origin can transform S. baltica and replicate in this bacterium. Without the antibiotic selection pressure plasmid maintenance is less efficient than in Escherichia coli. Nevertheless, cultivation of S. baltica in the presence of appropriate antibiotics caused relatively stable maintenance of ColE1-like and P1-derived plasmids. This indicates that plasmid-based genetic manipulations and gene transfer in S. baltica are possible.
authors.affiliation: Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Milewska
authors.affiliation: Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Węgrzyn
authors.affiliation: Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland. Electronic address: Agnieszka.Szalewska@biol.ug.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szalewska-Pałasz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004261:DNA Replication; D018628:Gene Dosage; D042822:Genomic Instability; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin; D020592:Shewanella / Q000235:genetics* / Q000254:growth & development*; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; C042833:ROM protein, Bacteria
keywords: ColE1; P1; Plasmid origin; Plasmid replication; Shewanella baltica; Transformation