title: Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?
abstract: Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.
authors.affiliation: Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland a_bajek@wp.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Bajek
authors.affiliation: Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Gurtowska
authors.affiliation: Chair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Gackowska
authors.affiliation: Chair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kubiszewska
authors.affiliation: Department of Clinical Pathomorphology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bodnar
authors.affiliation: Department of Clinical Pathomorphology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszałek
authors.affiliation: Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Januszewski
authors.affiliation: Chair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland Department of Microbiology and Immunology, The Children's Memorial Health Institute, 04-730 Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Michalkiewicz
authors.affiliation: Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland Department of Urology, Nicolaus Copernicus Hospital, 87-100 Torun, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
mesh_terms: D017667:Adipocytes; D050156:Adipogenesis / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D015187:Lipectomy / Q000379:methods*; D010012:Osteogenesis / Q000502:physiology; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D018084:Ultrasonography, Interventional / Q000379:methods
chemical_list: D015703:Antigens, CD; D015415:Biomarkers
keywords: adipose-derived stem cells; liposuction; markers' extensive analysis; phenotypic characterization