title: Three-dimensional mapping of fluorescent nanoparticles using incoherent digital holography.
abstract: Three-dimensional mapping of fluorescent nanoparticles was performed by using incoherent digital holography. The positions of the nanoparticles were quantitatively determined by using Gaussian fitting of the axial- and lateral-diffraction distributions through position calibration from the observation space to the sample space. It was found that the axial magnification was constant whereas the lateral magnification linearly depended on the axial position of the fluorescent nanoparticles. The mapping of multiple fluorescent nanoparticles fixed in gelatin and a single fluorescent nanoparticle manipulated with optical tweezers in water were demonstrated.
authors.forename: Takumi
authors.initials: T
authors.lastname: Yanagawa
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Abe
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hayasaki
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006696:Holography / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging
chemical_list: D005456:Fluorescent Dyes