title: Dynamic Expression of DNA Complexation with Self-assembled Biomolecular Clusters.
abstract: We report herein the implementation of a dynamic covalent chemistry approach to the generation of multivalent clusters for DNA recognition. We show that biomolecular clusters can be expressed in situ by a programmed self-assembly process using chemoselective ligations. The cationic clusters are shown, by fluorescence displacement assay, gel electrophoresis and isothermal titration calorimetry, to effectively complex DNA through multivalent interactions. The reversibility of the ligation was exploited to demonstrate that template effects occur, whereby DNA imposes component selection in order to favor the most active DNA-binding clusters. Furthermore, we show that a chemical effector can be used to trigger DNA release through component exchange reactions.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier, ENSCM, Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, 34296 Montpellier cedex 5 (France).
authors.forename: Eline
authors.initials: E
authors.lastname: Bartolami
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier, ENSCM, Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, 34296 Montpellier cedex 5 (France).
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bessin
authors.affiliation: CNRS; IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, BP64182, 31077 Toulouse (France).|Université de Toulouse; UPS; IPBS, 31077 Toulouse (France).
authors.forename: Virginie
authors.initials: V
authors.lastname: Gervais
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier, ENSCM, Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, 34296 Montpellier cedex 5 (France).
authors.forename: Pascal
authors.initials: P
authors.lastname: Dumy
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier, ENSCM, Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, 34296 Montpellier cedex 5 (France). Sebastien.Ulrich@enscm.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Ulrich
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D002412:Cations; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002412:Cations; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D004247:DNA
keywords: DNA recognition; bioconjugates; dynamic covalent chemistry; multivalency; self-assembly