title: Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean.
abstract: We identified and characterized a mutant of soybean stachyose synthase gene controlling reduced stachyose content which benefit the soybean seed composition breeding program in the future. It has been shown that in soybean, increased sucrose and reduced raffinose family oligosaccharides would have a positive impact on the world's feed industry by improving digestibility and feed efficiency. We searched for new sources of modified oligosaccharide content in a subset of the USDA Soybean Germplasm Collection and then identified plant introduction (PI) 603176A as having ultra-low stachyose content (0.5%). We identified a 33-bp deletion mutant in the putative stachyose synthase gene (STS gene, Glyma19g40550) of PI 603176A. A co-dominate indel marker was successfully developed from this 33-bp deletion area and was genetically mapped into two F 2:3 populations and a F 4:5 population, which associated with low stachyose content in the progeny lines. These observations provided strong evidence that the STS gene is responsible for stachyose biosynthesis in the soybean plant. Expression of the sts gene remained at the normal level, suggesting the loss of function in the gene is due to defective protein function. This gene-based perfect genetic marker for low stachyose content can be useful for marker-assisted selection in soybean molecular breeding programs.
authors.affiliation: Division of Plant Sciences, National Center for Soybean Biotechnology (NCSB), University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Qiu
authors.affiliation: Division of Plant Sciences, National Center for Soybean Biotechnology (NCSB), University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Tri
authors.initials: T
authors.lastname: Vuong
authors.affiliation: Division of Plant Sciences, National Center for Soybean Biotechnology (NCSB), University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Babu
authors.initials: B
authors.lastname: Valliyodan
authors.affiliation: Division of Plant Sciences, National Center for Soybean Biotechnology (NCSB), University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Haiying
authors.initials: H
authors.lastname: Shi
authors.affiliation: Division of Plant Sciences, National Center for Soybean Biotechnology (NCSB), University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Binhui
authors.initials: B
authors.lastname: Guo
authors.affiliation: Division of Plant Sciences and NCSB, University of Missouri, Portageville, MO, 63873, USA.
authors.forename: J Grover
authors.initials: JG
authors.lastname: Shannon
authors.affiliation: Division of Plant Sciences, National Center for Soybean Biotechnology (NCSB), University of Missouri, Columbia, MO, 65211, USA. nguyenhenry@missouri.edu.
authors.forename: Henry T
authors.initials: HT
authors.lastname: Nguyen
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D005700:Galactosyltransferases / Q000235:genetics*; D005819:Genetic Markers; D054643:INDEL Mutation; D009844:Oligosaccharides / Q000737:chemistry*; D000069600:Plant Breeding; D010940:Plant Proteins / Q000235:genetics*; D011887:Raffinose / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013025:Glycine max / Q000201:enzymology / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D009844:Oligosaccharides; D010940:Plant Proteins; C005695:stachyose; D005700:Galactosyltransferases; C113913:galactinol -raffinose galactosyltransferase; D011887:Raffinose