title: (1) H NMR Metabolic Profiling of Biofluids from Rats with Gastric Mucosal Lesion and Electroacupuncture Treatment.
abstract: Gastric mucosal lesion (GML) is a common gastrointestinal disorder with multiple pathogenic mechanisms in clinical practice. In traditional Chinese medicine (TCM), electroacupuncture (EA) treatment has been proven as an effective therapy for GML, although the underlying healing mechanism is not yet clear. Here, we used proton nuclear magnetic resonance- ((1)H NMR-) based metabolomic method to investigate the metabolic perturbation induced by GML and the therapeutic effect of EA treatment on stomach meridian (SM) acupoints. Clear metabolic differences were observed between GML and control groups, and related metabolic pathways were discussed by means of online metabolic network analysis toolbox. By comparing the endogenous metabolites from GML and GML-SM groups, the disturbed pathways were partly recovered towards healthy state via EA treated on SM acupoints. Further comparison of the metabolic variations induced by EA stimulated on SM and the control gallbladder meridian (GM) acupoints showed a quite similar metabolite composition except for increased phenylacetylglycine, 3,4-dihydroxymandelate, and meta-hydroxyphenylacetate and decreased N-methylnicotinamide in urine from rats with EA treated on SM acupoints. The current study showed the potential application of metabolomics in providing further insight into the molecular mechanism of acupuncture.
authors.affiliation: Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Bioprocess Engineering and Innovation Centre in Agritechnology, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia.
authors.forename: Kian-Kai
authors.initials: KK
authors.lastname: Cheng
authors.affiliation: Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Zongbao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Dong