title: Cholesterol-based polymeric monolithic columns for capillary liquid chromatography. Part II.
abstract: This paper presents a second part of our research devoted to cholesterol-based polymeric monolithic stationary phase. The obtained capillary columns were successfully used for separations of alkylbenzenes, steroid hormones and polycyclic aromatic hydrocarbons during isocratic or gradient elutions. The columns showed excellent thermal stability. Increasing the temperature resulted in decrease of the retention factors and peak widths, but selectivity and efficient separations have been retained in the studied temperature range of 20 to100°C. Additionally, the van't Hoff model presented a non-linear relationship of lnk versus 1/T plots, which is likely the result of liquid crystal properties of cholesterol. The studied capillary monolithic columns showed extremely fast re-equilibration after gradient elution and found to be stable under such conditions as: fast flow rate, high acetonitrile content in the mobile phase (no swelling) and high temperature.
authors.affiliation: Nicolaus Copernicus University in Toruń, Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, 7 Gagarin St., 87-100 Torun, Poland; Nicolaus Copernicus University in Toruń, Center for Modern Interdisciplinary Technologies, 4 Wilenska St., 87-100 Torun, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Grzywiński
authors.affiliation: Nicolaus Copernicus University in Toruń, Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, 7 Gagarin St., 87-100 Torun, Poland; Nicolaus Copernicus University in Toruń, Center for Modern Interdisciplinary Technologies, 4 Wilenska St., 87-100 Torun, Poland. Electronic address: michu@chem.umk.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Szumski
authors.affiliation: Nicolaus Copernicus University in Toruń, Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, 7 Gagarin St., 87-100 Torun, Poland; Nicolaus Copernicus University in Toruń, Center for Modern Interdisciplinary Technologies, 4 Wilenska St., 87-100 Torun, Poland.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000097:Acetonitriles; D002784:Cholesterol / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D012739:Gonadal Steroid Hormones / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D011108:Polymers / Q000737:chemistry; D013696:Temperature
chemical_list: D000097:Acetonitriles; D012739:Gonadal Steroid Hormones; D011084:Polycyclic Aromatic Hydrocarbons; D011108:Polymers; D002784:Cholesterol; C032159:acetonitrile
keywords: Capillary columns; Cholesterol stationary phase; Nano liquid chromatography; Polycyclic aromatic hydrocarbons; Polymeric monoliths; Steroid hormones