title: Quinolizidine alkaloids reduced mortality in EV71-infected mice by compensating for the levels of T cells.
abstract: Recent outbreak of hand, foot and mouse disease caused by enterovirus 71 is a serious threat to infants and children in Asia-Pacific countries. No vaccines or antiviral therapies are available against this infection. In this study, we found that quinolizidine alkaloids could improve the levels of T cells in mice. The structure-activity relationships demonstrated that the oxidation and double bonds are important for the excellent potency. Oxysophocarpine, the most effective compound of six quinolizidine alkaloids, could also relieve symptoms and reduce mortality in lethal enterovirus 71-infected mice through compensating for the decreased levels of T cells. This work suggested that quinolizidine alkaloids have the potential against enterovirus 71 for further development of novel antiviral drugs.
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China; State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, and Beijing Key Laboratory of Active Substances Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Guan
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Bai
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Jiangning
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, and Beijing Key Laboratory of Active Substances Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: xdp@imm.ac.cn.
authors.forename: Xiandao
authors.initials: X
authors.lastname: Pan
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China. Electronic address: zhanglf@cnilas.org.
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000627:therapeutic use*; D029821:Enterovirus A, Human / Q000187:drug effects* / Q000276:immunology; D006232:Hand, Foot and Mouth Disease / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D008807:Mice, Inbred BALB C; D054837:Quinolizidines / Q000737:chemistry / Q000627:therapeutic use*; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000998:Antiviral Agents; D054837:Quinolizidines; C046246:oxysophocarpine; D000093842:Matrines
keywords: Enterovirus 71; Hand, foot and mouth disease; Quinolizidine alkaloids; T cells