title: Screening of stabilizers for LC-MS/MS analysis of clevidipine and its primary metabolite in dog whole blood.
abstract: BACKGROUND
  Clevidipine is an ester-containing antihypertensive agent that undergoes rapid hydrolysis in blood. A reliable stabilizer cocktail containing citric acid and ascorbic acid was established and the LC-MS/MS method was validated for simultaneous determination of clevidipine and its major metabolite in beagle dog whole blood.
  
  
  RESULTS
  The stabilizer could nearly completely inhibit the esterase activity. Both analytes were extracted from whole blood by toluene and detected by MS/MS in positive ESI mode. The linearity range was 0.1-100.0 ng/ml for clevidipine and 1.0-1000.0 ng/ml for the primary metabolite.
  
  
  CONCLUSIONS
  The stabilizer cocktail was able to effectively suppress the activity of esterase in blood. The method was successfully applied to a PK study of clevidipine in beagle dogs.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.|Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Gao
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Shouwei
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.|Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Luqin
authors.initials: L
authors.lastname: Si
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China.|Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan 430030, PR China.
authors.forename: Jiangeng
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002121:Calcium Channel Blockers / Q000097:blood* / Q000378:metabolism / Q000592:standards; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004285:Dogs; D011725:Pyridines / Q000097:blood* / Q000378:metabolism / Q000592:standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards
chemical_list: D002121:Calcium Channel Blockers; D011725:Pyridines; C118563:clevidipine