title: Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging.
abstract: Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable genetically encoded Ca(2+) indicators that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in a single-color protein sensor. We demonstrated in cultured neurons and in fruit fly and zebrafish larvae how single cells could be selected out of dense populations for visualization of morphology and high signal-to-noise measurements of activity, synaptic transmission and connectivity. Our design strategy is transferrable to other sensors based on circularly permutated GFP (cpGFP).
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.|Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Shai
authors.initials: S
authors.lastname: Berlin
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Carroll
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Zachary L
authors.initials: ZL
authors.lastname: Newman
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Hitomi O
authors.initials: HO
authors.lastname: Okada
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Carson M
authors.initials: CM
authors.lastname: Quinn
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.|Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kallman
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, USA.
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Rockwell
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, USA.
authors.forename: Shelley S
authors.initials: SS
authors.lastname: Martin
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, USA.
authors.forename: J Clark
authors.initials: JC
authors.lastname: Lagarias
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.|Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California, USA.|Physical Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Ehud Y
authors.initials: EY
authors.lastname: Isacoff
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods; D002478:Cells, Cultured; D004330:Drosophila; D008027:Light; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D062308:Optogenetics / Q000379:methods*; D015202:Protein Engineering / Q000379:methods; D051381:Rats; D015027:Zebrafish
chemical_list: D008164:Luminescent Proteins; D002118:Calcium