title: [Construction and Characterization of B850-Only LH2 Energy Transfer System in Purple Bacteria].
abstract: To seek microscopic molecular mechanism of energy transfer and complex reconstitution in the photosynthesis, the conditions for construction of B850-only peripheral light-harvesting complex (LH2) and their properties were investigated using absorption, fluorescence spectroscopy, molecular sieve chromatography, ultrafiltration and sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) from the purple bacteria. The results indicated that bacteriochlorophylls (BChl) of B800 incubated in 10 mmo · L(-1) Tris-HCl (pH 8.0) buffer are selectively released from their binding sites of LH2 of Rhodobacter azotoformans (A-LH2) by 0.08% (W/V) SDS. B850-only A-LH2 was constructed after removing free BChl mixing with 10% methyl alcohol by ultrafiltration. B850 BChl was released after A-LH2 was incubated for 240 min in dark at room temperature (RT). While BChl of B800 incubated in pH 1.9 buffer were selectively released from their binding sites of LH2 of Rhodopseudomonas palustris (P-LH2). The authors acquired two components using molecular sieve chromatography. Free BChl of one component was not removed and self-assembled to P-LH2. The other removed free BChl and B850-only P-LH2 was constructed. B850 unchanged after P-LH2 was incubated. P-LH2 α and β subunits have different molecular weights, but those of A-LH2 are in the contrary. It is concluded that B850-only P-LH2 is more stable than A-LH2. The enigmatic split of the B800 absorption band was not observed in these LH2, but we acquired two kinds of B800-released LH2 from Rhodopseudomonas palustris. The authors' results may provide a new light to separate homogeneous Apoprotein LH2.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Chun-gui
authors.initials: CG
authors.lastname: Zhao
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Yue
authors.forename: Su-ping
authors.initials: SP
authors.lastname: Yang
authors.forename: Yin-bo
authors.initials: YB
authors.lastname: Qu
authors.forename: Nian-zhi
authors.initials: NZ
authors.lastname: Jiao
mesh_terms: D001429:Bacteriochlorophylls / Q000737:chemistry; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D004735:Energy Transfer; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D010788:Photosynthesis; D012241:Rhodopseudomonas / Q000737:chemistry*
chemical_list: D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes