title: Anti-inflammatory Flavonoids Isolated from Passiflora foetida.
abstract: In this study, we evaluated the anti-inflammatory activity of the soluble ethyl acetate fraction and chemical components of the stem bark of Passiflora foetida (Passifloraceae). Ten flavonoids (1-10) were isolated by various chromatographic techniques, and their structures were determined based on spectroscopic analyses by using nuclear magnetic resonance (NMR). Luteolin (2) and chrysoeriol (3) showed the most potent inhibition of nitric oxide (NO) production in macrophage cell line, RAW264.7, with half maximal inhibitor concentration (IC50) values of 1.2 and 3.1 μM, respectively. These compounds suppressed lipopolysaccharide (LPS)-induced inducible NO synthase (iNOS) expression at the transcription level. Our research indicates that the stem bark of P. foetida has significant anti-inflammatory properties, suggesting that its flavonoids may have anti-inflammatory benefits.
authors.forename: Thi Yen
authors.initials: TY
authors.lastname: Nguyen
authors.forename: Dao Cuong
authors.initials: DC
authors.lastname: To
authors.forename: Manh Hung
authors.initials: MH
authors.lastname: Tran
authors.forename: Joo Sang
authors.initials: JS
authors.lastname: Lee
authors.forename: Jeong Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D009569:Nitric Oxide / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D029598:Passiflora / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D016328:NF-kappa B; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II