title: Hypothyroidism Exacerbates Thrombophilia in Female Rats Fed with a High Fat Diet.
abstract: Clotting abnormalities are discussed both in the context with thyroid dysfunctions and obesity caused by a high fat diet. This study aimed to investigate the impact of hypo-, or hyperthyroidism on the endogenous thrombin potential (ETP), a master indicator of clotting activation, on Sprague Dawley rats fed a normal or high fat diet. Female Sprague Dawley rats (n = 66) were grouped into normal diet (ND; n = 30) and high-fat diet (HFD; n = 36) groups and subdivided into controls, hypothyroid and hyperthyroid groups, induced through propylthiouracil or triiodothyronine (T3) treatment, respectively. After 12 weeks of treatment ETP, body weight and food intake were analyzed. Successfully induced thyroid dysfunction was shown by T3 levels, both under normal and high fat diet. Thyroid dysfunction was accompanied by changes in calorie intake and body weight. In detail, compared to euthyroid controls, hypothyroid rats showed significantly increased-and hyperthyroid animals significantly decreased-ETP levels. High fat diet potentiated these effects in both directions. In summary, we are the first to show that hypothyroidism and high fat diet potentiate the thrombotic capacity of the clotting system in Sprague Dawley rats. This effect may be relevant for cardiovascular disease where thyroid function is poorly understood as a pathological contributor in the context of clotting activity and obesogenic nutrition.
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. harald.mangge@klinikum-graz.at.
authors.forename: Harald
authors.initials: H
authors.lastname: Mangge
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. Florian.Prueller@klinikum-graz.at.
authors.forename: Florian
authors.initials: F
authors.lastname: Prüller
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. sieglinde.zelzer@klinikum-graz.at.
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Zelzer
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. Herwig.Ainoedhofer@klinikum-graz.at.
authors.forename: Herwig
authors.initials: H
authors.lastname: Ainödhofer
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. sabine.pailer@klinikum-graz.at.
authors.forename: Sabine
authors.initials: S
authors.lastname: Pailer
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. Petra.Kieslinger@klinikum-graz.at.
authors.forename: Petra
authors.initials: P
authors.lastname: Kieslinger
authors.affiliation: Department of Pathology, Medical University of Graz, 8036 Graz, Austria. johannes.haybaeck@medunigraz.at.
authors.forename: Johannes
authors.initials: J
authors.lastname: Haybaeck
authors.affiliation: Division of Endocrinology and Metabolism, Medical University Graz, 8036 Graz, Austria. barbara.obermayer@medunigraz.at.
authors.forename: Barbara
authors.initials: B
authors.lastname: Obermayer-Pietsch
authors.affiliation: Physiological Chemistry, Medical University of Graz, 8036 Graz, Austria. gerhard.cvirn@medunigraz.at.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Cvirn
authors.affiliation: Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University Graz, 8036 Graz, Austria. hans.gruber@medunigraz.at.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Gruber
mesh_terms: D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat; D004435:Eating; D005260:Female; D006980:Hyperthyroidism / Q000139:chemically induced / Q000150:complications / Q000473:pathology; D007037:Hypothyroidism / Q000139:chemically induced / Q000150:complications / Q000473:pathology*; D011441:Propylthiouracil / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D019851:Thrombophilia / Q000209:etiology*; D014284:Triiodothyronine / Q000633:toxicity
chemical_list: D014284:Triiodothyronine; D011441:Propylthiouracil
keywords: Sprague Dawley rats; endogenous thrombin potential (ETP); fat feeding; hyperthyroidism; hypothyroidism