title: 'Spotted Nanoflowers': Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture.
abstract: Hybrid gold nanostructures seeded into nanotextured zinc oxide (ZnO) nanoflowers (NFs) were created for novel biosensing applications. The selected 'spotted NFs' had a 30-nm-thick gold nanoparticle (AuNP) layer, chosen from a range of AuNP thicknesses, sputtered onto the surface. The generated nanohybrids, characterized by morphological, physical and structural analyses, were uniformly AuNP-seeded onto the ZnO NFs with an average length of 2-3 μm. Selective capture of molecular probes onto the seeded AuNPs was evidence for the specific interaction with DNA from pathogenic Leptospirosis-causing strains via hybridization and mis-match analyses. The attained detection limit was 100 fM as determined via impedance spectroscopy. High levels of stability, reproducibility and regeneration of the sensor were obtained. Selective DNA immobilization and hybridization were confirmed by nitrogen and phosphorus peaks in an X-ray photoelectron spectroscopy analysis. The created nanostructure hybrids illuminate the mechanism of generating multiple-target, high-performance detection on a single NF platform, which opens a new avenue for array-based medical diagnostics.
authors.affiliation: Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia.
authors.forename: Veeradasan
authors.initials: V
authors.lastname: Perumal
authors.affiliation: 1] Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia [2] School of Microelectronic Engineering, University Malaysia Perlis (UniMAP), Kuala Perlis, Perlis, Malaysia.
authors.forename: U
authors.initials: U
authors.lastname: Hashim
authors.affiliation: Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia.
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.affiliation: Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Haarindraprasad
authors.affiliation: Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia.
authors.forename: K L
authors.initials: KL
authors.lastname: Foo
authors.affiliation: Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia.
authors.forename: S R
authors.initials: SR
authors.lastname: Balakrishnan
authors.affiliation: School of Microelectronic Engineering, University Malaysia Perlis (UniMAP), Kuala Perlis, Perlis, Malaysia.
authors.forename: P
authors.initials: P
authors.lastname: Poopalan
mesh_terms: D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D056951:Photoelectron Spectroscopy; D013052:Spectrometry, X-Ray Emission; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D006046:Gold; D015034:Zinc Oxide