title: Separation of polyphenols and caffeine from the acetone extract of fermented tea leaves (Camellia sinensis) using high-performance countercurrent chromatography.
abstract: Leaves from Camellia sienensis are a popular natural source of various beverage worldwide, and contain caffeine and polyphenols derived from catechin analogues. In the current study, caffeine (CAF, 1) and three tea polyphenols including (-)-epigallocatechin 3-O-gallate (EGCg, 2), (-)-gallocatechin 3-O-gallate (GCg, 3), and (-)-epicatechin 3-O-gallate (ECg, 4) were isolated and purified by flow-rate gradient high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:9:1:9, v/v). Two hundred milligrams of acetone-soluble extract from fermented C. sinensis leaves was separated by HPCCC to give 1 (25.4 mg), 2 (16.3 mg), 3 (11.1 mg) and 4 (4.4 mg) with purities over 98%. The structures of 1-4 were elucidated by QTOF-MS, as well as 1H- and 13C-NMR, and the obtained data were compared to the previously reported values.
authors.affiliation: College of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 420-743, Korea. wjd6694@naver.com.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea. hydhong@amorepacific.com.
authors.forename: Yong Deog
authors.initials: YD
authors.lastname: Hong
authors.affiliation: AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea. bjlee@amorepacific.com.
authors.forename: Bumjin
authors.initials: B
authors.lastname: Lee
authors.affiliation: AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea. superbody@amorepacific.com.
authors.forename: Jun Seong
authors.initials: JS
authors.lastname: Park
authors.affiliation: AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea. misterjay@amorepacific.com.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Jeong
authors.affiliation: AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea. katemina@amorepacific.com.
authors.forename: Wan Gi
authors.initials: WG
authors.lastname: Kim
authors.affiliation: AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea. ssshin@amorepacific.com.
authors.forename: Song Seok
authors.initials: SS
authors.lastname: Shin
authors.affiliation: College of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 420-743, Korea. kdyoon@catholic.ac.kr.
authors.forename: Kee Dong
authors.initials: KD
authors.lastname: Yoon
mesh_terms: D000096:Acetone / Q000737:chemistry*; D002110:Caffeine / Q000737:chemistry / Q000302:isolation & purification*; D028241:Camellia sinensis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D059808:Polyphenols; D000096:Acetone; D002110:Caffeine
keywords: Camellia sinensis; high-performance countercurrent chromatography; tea-polyphenols