title: Myosin 1b functions as an effector of EphB signaling to control cell repulsion.
abstract: Eph receptors and their membrane-tethered ligands, the ephrins, have important functions in embryo morphogenesis and in adult tissue homeostasis. Eph/ephrin signaling is essential for cell segregation and cell repulsion. This process is accompanied by morphological changes and actin remodeling that drives cell segregation and tissue patterning. The actin cortex must be mechanically coupled to the plasma membrane to orchestrate the cell morphology changes. Here, we demonstrate that myosin 1b that can mechanically link the membrane to the actin cytoskeleton interacts with EphB2 receptors via its tail and is tyrosine phosphorylated on its tail in an EphB2-dependent manner. Myosin 1b regulates the redistribution of myosin II in actomyosin fibers and the formation of filopodia at the interface of ephrinB1 and EphB2 cells, which are two processes mediated by EphB2 signaling that contribute to cell repulsion. Together, our results provide the first evidence that a myosin 1 functions as an effector of EphB2/ephrinB signaling, controls cell morphology, and thereby cell repulsion.
authors.affiliation: Institut Curie, Centre de Recherche, F-75248 Paris, France.
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Prospéri
authors.affiliation: Institut Curie, Centre de Recherche, F-75248 Paris, France Université Pierre et Marie Curie, F-75252 Paris, France.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Lépine
authors.affiliation: Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, Centre de Recherche, F-75248 Paris, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Dingli
authors.affiliation: Institut Curie, Centre de Recherche, F-75248 Paris, France Cell and Tissue Imaging Facility (PICT-IBiSA), Centre National de la Recherche Scientifique, UMR 144, Paris F-75248, France.
authors.forename: Perrine
authors.initials: P
authors.lastname: Paul-Gilloteaux
authors.affiliation: Department of Chemistry, Technische Univesität, 01069 Dresden, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Martin
authors.affiliation: Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, Centre de Recherche, F-75248 Paris, France.
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.affiliation: Department of Chemistry, Technische Univesität, 01069 Dresden, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Knölker
authors.affiliation: Institut Curie, Centre de Recherche, F-75248 Paris, France Cell and Tissue Imaging Facility (PICT-IBiSA), Centre National de la Recherche Scientifique, UMR 144, Paris F-75248, France coudrier@curie.fr.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Coudrier
mesh_terms: D000595:Amino Acid Sequence; D002450:Cell Communication; D036387:Ephrin-B1 / Q000502:physiology; D045325:HCT116 Cells; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D024461:Myosin Type I / Q000502:physiology*; D024462:Myosin Type II / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011554:Pseudopodia / Q000502:physiology; D036183:Receptor, EphB2 / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C484288:EFNB1 protein, human; D036387:Ephrin-B1; C000594736:MYO1B protein, human; C586310:EPHB2 protein, human; D036183:Receptor, EphB2; D024461:Myosin Type I; D024462:Myosin Type II