title: Isolation and characterization of CD105+/CD90+ subpopulation in breast cancer MDA-MB-231 cell line.
abstract: BACKGROUND
  The epithelial-mesenchymal transition (EMT) generates cells with properties of stem cells, if that happened, the stem cell should be with mesenchymal property. This study aimed to identify a group of cells with mesenchymal stem cell (MSC)-like characteristics in breast cancer bone metastatic cell line MDA-MB-231, moreover, the relevance between breast cancer stem cells and the EMT was observed. CD105 and CD90, identified as the standards of MSCs, were used for the identification.
  
  
  METHODS
  The CD105+/CD90+ and CD105-/CD90- subpopulation of MDA-MB-231 cells were detected and sorted by flow cytometry. MSC-like characteristics in cell proliferation, migration and cell cycle were investigated here by MTT asaay, transwell migration assay, and PI staining respectively. The expression profiles of some stem cell-associated genes were also observed by quantitative real time PCR.
  
  
  RESULTS
  Around 0.99% and 90.77% of parental cells were identified as CD105+/CD90+ and CD105-/CD90- cell subpopulations respectively. The CD105+/CD90+ cells exhibited stronger migratory capacity as compared to parental and CD105-/CD90- cells, while less CD105+/CD90+ cells were arrested in the S phase. Besides, pluripotent stem cell factors, like Oct-4, Nanog, Klf4 and Sox-2, were all upregulated in CD105+/CD90+ cells, with also proliferation increase, as compared with other two populations.
  
  
  CONCLUSIONS
  The CD105+/CD90+ subpopulation from breast cancer MDA-MB-231 cells was proven to possess "mesenchymal stem cell-like" characteristics, and its high migratory ability might be associated with EMT. Moreover, using the surface markers of CD105 and CD90 for the identification of MSCs might provide new theoretical basis for the recurrence and metastasis of breast cancer.
authors.affiliation: Department of Dermatologic Surgery, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, P. R. China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Hebei Breast Disease Diagnosis and Treatment Center, The Fourth Hospital of Hebei Medical University Tianshan Road, Shijiazhuang 050035, Hebei, P. R. China.
authors.forename: Yunjiang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Research Center, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, P. R. China.
authors.forename: Kaixuan
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Research Center, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, P. R. China.
authors.forename: Geng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Clinical laboratory, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, P. R. China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Clinical laboratory, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, P. R. China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Ren
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D000071063:Endoglin; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000090062:Kruppel-Like Factor 4; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C064206:ENG protein, human; D000071063:Endoglin; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens
keywords: Breast neoplasm; CD105; CD90; cancer stem cell; epithelial mesenchymal transition; mesenchymal stem cells