title: Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications.
abstract: Although antibiotics have been widely used in clinical applications to treat pathogenic infections at present, the problem of drug-resistance associated with abuse of antibiotics is becoming a potential threat to human beings. We report a biohybrid nanomaterial consisting of antibiotics, enzyme, polymers, hyaluronic acid (HA), and mesoporous silica nanoparticles (MSNs), which exhibits efficient in vitro and in vivo antibacterial activity with good biocompatibility and negligible hemolytic side effect. Herein, biocompatible layer-by-layer (LBL) coated MSNs are designed and crafted to release encapsulated antibiotics, e.g., amoxicillin (AMO), upon triggering with hyaluronidase, produced by various pathogenic Staphylococcus aureus (S. aureus). The LBL coating process comprises lysozyme (Lys), HA, and 1,2-ethanediamine (EDA)-modified polyglycerol methacrylate (PGMA). The Lys and cationic polymers provided multivalent interactions between MSN-Lys-HA-PGMA and bacterial membrane and accordingly immobilized the nanoparticles to facilitate the synergistic effect of these antibacterial agents. Loading process was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction spectroscopy (XRD). The minimal inhibition concentration (MIC) of MSN-Lys-HA-PGMA treated to antibiotic resistant bacteria is much lower than that of isodose Lys and AMO. Especially, MSN-Lys-HA-PGMA exhibited good inhibition for pathogens in bacteria-infected wounds in vivo. Therefore, this type of new biohybrid nanomaterials showed great potential as novel antibacterial agents.
authors.affiliation: †School of Chemistry and Chemical Engineering, School of Material Science and Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Yuanhao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: †School of Chemistry and Chemical Engineering, School of Material Science and Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Long
authors.affiliation: ‡State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), Jilin University, 2699 Qianjin Street, Changchun 130012, People's Republic of China.
authors.forename: Qing-Lan
authors.initials: QL
authors.lastname: Li
authors.affiliation: §Department of Pharmacology, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063000, People's Republic of China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Han
authors.affiliation: †School of Chemistry and Chemical Engineering, School of Material Science and Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Jianbiao
authors.initials: J
authors.lastname: Ma
authors.affiliation: ‡State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), Jilin University, 2699 Qianjin Street, Changchun 130012, People's Republic of China.
authors.forename: Ying-Wei
authors.initials: YW
authors.lastname: Yang
authors.affiliation: †School of Chemistry and Chemical Engineering, School of Material Science and Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
mesh_terms: D000658:Amoxicillin / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D000067493:Dynamic Light Scattering; D005029:Ethylenediamines / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013818:Thermogravimetry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D005029:Ethylenediamines; D011108:Polymers; C031234:ethylenediamine; D012822:Silicon Dioxide; D000658:Amoxicillin; D006820:Hyaluronic Acid; D009113:Muramidase
keywords: MSN; antibacterial materials; biohybrid nanoparticles; cationic polymers; enzyme response; layer-by-layer self-assembly; lysozyme; synergistic effects