title: Measuring Serum Amyloid A for Infection Prediction in Aneurysmal Subarachnoid Hemorrhage.
abstract: Aneurysmal subarachnoid hemorrhage (aSAH) is associated with high rates of mortality and morbidity. Nosocomial infections, such as pneumonia or urinary tract infections, are among the main causes of worsening outcomes and death. The aim of this study was to discover a biomarker to predict infection in aSAH patients. For this purpose, the plasma of infected and noninfected patients was compared using quantitative mass spectrometry. The most interesting differentially expressed proteins were selected for validation by immunoassays on plasma samples taken from patients (n = 81) over 10 days of hospitalization. Predictive performances were established using Mann-Whitney U tests and receiver operating characteristic curves. Quantitative proteomics identified 17 significantly regulated proteins. Of these, levels of serum amyloid A (SAA) were significantly higher in infected patients (p < 0.007). ELISA confirmed that the concentrations were significantly higher (p < 0.002) already at hospital admission in patients who subsequently developed an infection during their hospitalization, (AUC of 76%) for a cutoff value of 90.9 μg/mL. Our data suggested that measuring SAA could be an efficient means of detecting patients susceptible of developing an infection during hospitalization after an aSAH. Its predictive capacity could lead to earlier antibiotherapy, improved patient management, and potentially better long-term outcomes.
authors.affiliation: Department of Human Protein Sciences, University of Geneva , Rue Michel Servet 1, CH-1211 Geneva, Switzerland.
authors.forename: Leire
authors.initials: L
authors.lastname: Azurmendi
authors.affiliation: Department of Anesthesiology, Pitié-Salpêtrière University Hospital , 47-83 Boulevard de l'Hôpital, 75013 Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Degos
authors.affiliation: Department of Pathology, University of Sydney , Sydney NSW 2006, Australia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Tiberti
authors.affiliation: Department of Anesthesiology, Pitié-Salpêtrière University Hospital , 47-83 Boulevard de l'Hôpital, 75013 Paris, France.
authors.forename: Natacha
authors.initials: N
authors.lastname: Kapandji
authors.affiliation: Department of Anesthesiology, Pitié-Salpêtrière University Hospital , 47-83 Boulevard de l'Hôpital, 75013 Paris, France.
authors.forename: Paola
authors.initials: P
authors.lastname: Sanchez
authors.affiliation: Department of Neurosurgery, Heidelberg University Hospital , Im Neuenheimer Feld 400, 69120 Heidelberg, Germany.
authors.forename: Asita
authors.initials: A
authors.lastname: Sarrafzadeh
authors.affiliation: Department of Anesthesiology, Pitié-Salpêtrière University Hospital , 47-83 Boulevard de l'Hôpital, 75013 Paris, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Puybasset
authors.affiliation: Department of Human Protein Sciences, University of Geneva , Rue Michel Servet 1, CH-1211 Geneva, Switzerland.
authors.forename: Natacha
authors.initials: N
authors.lastname: Turck
authors.affiliation: Department of Human Protein Sciences, University of Geneva , Rue Michel Servet 1, CH-1211 Geneva, Switzerland.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sanchez
mesh_terms: D000328:Adult; D000368:Aged; D003428:Cross Infection / Q000097:blood* / Q000150:complications; D005260:Female; D006760:Hospitalization; D006801:Humans; D002532:Intracranial Aneurysm / Q000097:blood* / Q000150:complications; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D015203:Reproducibility of Results; D000685:Serum Amyloid A Protein / Q000032:analysis*; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000150:complications
chemical_list: D020543:Proteome; D000685:Serum Amyloid A Protein
keywords: Glasgow Coma Scale; Glasgow Outcome Scale; collision-induced dissociation; nosocomial infection; serum amyloid A