title: Characteristic expression ofMSX1,MSX2,TBX2andENTPD1in dental pulp cells.
abstract: Dental pulp cells (DPCs) are a promising source of transplantable cells in regenerative medicine. However, DPCs have not been fully characterized at the molecular level. The aim of the present study was to distinguish DPCs from various source-derived mesenchymal stem cells (MSCs), fibroblasts (FBs) and other cells by the expression of several DPC-characteristic genes. DPCs were isolated from human pulp tissues by the explant method or the enzyme digestion method, and maintained with media containing 10% serum or 7.5% platelet-rich plasma. RNA was isolated from the cells and from dental pulp tissue specimens. The mRNA levels were determined by DNA microarray and quantitative polymerase chain reaction analyses. The msh homeobox 1, msh homeobox 2, T-box 2 and ectonucleoside triphosphate diphosphohydrolase 1 mRNA levels in DPCs were higher than that of the levels identified in the following cell types: MSCs derived from bone marrow, synovium and adipose tissue; and in cells such as FBs, osteoblasts, adipocytes and chondrocytes. The enhanced expression in DPCs was consistently observed irrespective of donor age, tooth type and culture medium. In addition, these genes were expressed at high levels in dental pulp tissuein vivo. In conclusion, this gene set may be useful in the identification and characterization of DPCs in basic studies and pulp cell-based regeneration therapy.
authors.affiliation: Department of Dental Science for Health Promotion, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Sakiko
authors.initials: S
authors.lastname: Fujii
authors.affiliation: Department of Dental and Medical Biochemistry, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Fujimoto
authors.affiliation: Department of Pediatric Dentistry, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Goto
authors.affiliation: Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Kanawa
authors.affiliation: Department of Dental and Medical Biochemistry, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawamoto
authors.affiliation: Two Cells Co., Ltd., Hiroshima, Hiroshima 734-8551, Japan.
authors.forename: Haiou
authors.initials: H
authors.lastname: Pan
authors.affiliation: BioEden Asia Co., Ltd., Klong Luang, Pathum Thani 12120, Thailand.
authors.forename: Petcharin
authors.initials: P
authors.lastname: Srivatanakul
authors.affiliation: BioEden Asia Co., Ltd., Klong Luang, Pathum Thani 12120, Thailand.
authors.forename: Waralak
authors.initials: W
authors.lastname: Rakdang
authors.affiliation: BioEden Asia Co., Ltd., Klong Luang, Pathum Thani 12120, Thailand.
authors.forename: Juthamas
authors.initials: J
authors.lastname: Pornprasitwech
authors.affiliation: Department of Pediatric Dentistry, Airlangga University, Surabaya, East Java 60132, Indonesia.
authors.forename: Tania
authors.initials: T
authors.lastname: Saskianti
authors.affiliation: Department of Conservative Dentistry, Airlangga University, Surabaya, East Java 60132, Indonesia.
authors.forename: Ketut
authors.initials: K
authors.lastname: Suardita
authors.affiliation: Department of Dental Science for Health Promotion, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Fusanori
authors.initials: F
authors.lastname: Nishimura
authors.affiliation: Department of Dental and Medical Biochemistry, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734-8553, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
keywords: dental pulp cells; fibroblasts; gene expression; mesenchymal stem cells; regenerative medicine