title: Alternative NF-κB Isoforms in the Drosophila Neuromuscular Junction and Brain.
abstract: The Drosophila NF-κB protein Dorsal is expressed at the larval neuromuscular junction, where its expression appears unrelated to known Dorsal functions in embryonic patterning and innate immunity. Using confocal microscopy with domain-specific antisera, we demonstrate that larval muscle expresses only the B isoform of Dorsal, which arises by intron retention. We find that Dorsal B interacts with and stabilizes Cactus at the neuromuscular junction, but exhibits Cactus independent localization and an absence of detectable nuclear translocation. We further find that the Dorsal-related immune factor Dif encodes a B isoform, reflecting a conservation of B domains across a range of insect NF-κB proteins. Carrying out mutagenesis of the Dif locus via a site-specific recombineering approach, we demonstrate that Dif B is the major, if not sole, Dif isoform in the mushroom bodies of the larval brain. The Dorsal and Dif B isoforms thus share a specific association with nervous system tissues as well as an alternative protein structure.
authors.affiliation: Section of Cell & Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Section of Cell & Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lindsay
authors.affiliation: Section of Cell & Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Wasserman
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004330:Drosophila; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000378:metabolism*
chemical_list: D016328:NF-kappa B