title: Effects of vitamin D on insulin secretion and glucose transporter GLUT2 under static magnetic field in rat.
abstract: The present study investigated the effects of vitamin D supplementation on insulin secretion and glucose transporter following static magnetic field (SMF) exposure in rat. Wistar male rats were divided into the following groups: control, SMF-exposed rat (128 mT; 1 h/day for 5 days), vitamin D-treated rats (1600 IU/100 g, received by gavage for five consecutive days), and co-exposed rats (the last day and after exposure rats received a single dose of vitamin D per os). Our results showed that exposure to SMF induced an increase in plasma glucose level and a decrease in plasma insulin concentration. Moreover, β cell insulin content and islet area were lower in SMF-exposed group compared to control. Likewise, we reported the absence of GLUT2 expression in extracellular membrane of pancreatic islet in SMF-exposed group. Interestingly, supplementation with single dose of vitamin D per os corrected insulinemia and glycemia disturbances caused by SMF. By contrast, the same treatment failed to correct pancreatic area. This study provides evidence that vitamin D supplementation has curative effect on pancreas insulin content and on GLUT2 disruption caused by SMF exposure.
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, 7021, Jarzouna, Tunisia. aida_lagui@yahoo.fr.|Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium. aida_lagui@yahoo.fr.
authors.forename: A
authors.initials: A
authors.lastname: Lahbib
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Ghodbane
authors.affiliation: Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Louchami
authors.affiliation: Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Sener
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Sakly
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D051273:Glucose Transporter Type 2 / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D061385:Insulins / Q000097:blood / Q000378:metabolism*; D060526:Magnetic Fields; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014807:Vitamin D / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D051273:Glucose Transporter Type 2; D061385:Insulins; D014807:Vitamin D
keywords: GLUT2 labeling; Insulin labeling; Pancreatic islet surface; Rats; SMF; Vitamin D supplementation