title: Prostanoids are not involved in postocclusive reactive hyperaemia in human skin.
abstract: Several mediators contribute to postocclusive reactive hyperaemia (PORH) in the skin, including sensory nerves and endothelium-derived hyperpolarizing factors. The main objective of this study was to investigate the specific involvement of prostanoids in human skin PORH. We tested the effect of the inhibition of cyclo-oxygenases (COX) by 4 mm ketoprofen, infused through microdialysis fibers inserted into the healthy volunteers forearm skin, following 5 min brachial artery occlusion. Skin microvascular blood flux was recorded using two-dimensional Laser Speckle Contrast Imaging. Maximal cutaneous vascular conductance (CVCmax ) was obtained following the perfusion of 29 mm sodium nitroprusside. A systematic review of the effects of COX inhibitors on skin peak PORH was also performed. We observed no significant difference between ketoprofen and placebo for the PORH peak (78 ± 8 and 71 ± 19% CVCmax , respectively) and area under the curve (2951 ± 721 and 2490 ± 936% CVCmax .s). A meta-analysis showed a substantial heterogeneity between studies, with overall a neutral effect of COX inhibition on peak PORH. Cyclo-oxygenase inhibition does not alter skin PORH, suggesting no involvement of prostanoids in cutaneous postocclusive vasodilatation in healthy humans.
authors.affiliation: Clinical Pharmacology Department, Inserm CIC3, University Hospital, Grenoble, France.|Noninvasive Cardiac Diagnostics Department, Medical University, Gdansk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Hellmann
authors.affiliation: Clinical Pharmacology Department, Inserm CIC3, University Hospital, Grenoble, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Gaillard-Bigot
authors.affiliation: Clinical Pharmacology Department, Inserm CIC3, University Hospital, Grenoble, France.|Inserm U1042, Grenoble, France.|University Grenoble-Alpes, Grenoble, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Roustit
authors.affiliation: Clinical Pharmacology Department, Inserm CIC3, University Hospital, Grenoble, France.|Inserm U1042, Grenoble, France.|University Grenoble-Alpes, Grenoble, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cracowski
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D001783:Blood Flow Velocity; D001916:Brachial Artery / Q000503:physiopathology*; D016009:Chi-Square Distribution; D016861:Cyclooxygenase Inhibitors / Q000008:administration & dosage; D005260:Female; D006801:Humans; D006940:Hyperemia / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D007511:Ischemia / Q000209:etiology / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry; D008297:Male; D017551:Microdialysis; D011453:Prostaglandins / Q000378:metabolism*; D011897:Random Allocation; D012039:Regional Blood Flow; D015398:Signal Transduction; D012867:Skin / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D014111:Tourniquets; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000008:administration & dosage; D055815:Young Adult
chemical_list: D016861:Cyclooxygenase Inhibitors; D011453:Prostaglandins; D014665:Vasodilator Agents
keywords: cyclo-oxygenase; endothelium; ketoprofen; laser speckle contrast imaging; microcirculation; postocclusive reactive hyperaemia; skin microdialysis