title: Bioactive Seed Layer for Surface-Confined Self-Assembly of Peptides.
abstract: The design and control of molecular systems that self-assemble spontaneously and exclusively at or near an interface represents a real scientific challenge. We present here a new concept, an active seed layer that allows to overcome this challenge. It is based on enzyme-assisted self-assembly. An enzyme, alkaline phosphatase, which transforms an original peptide, Fmoc-FFY(PO4 (2-) ), into an efficient gelation agent by dephosphorylation, is embedded in a polyelectrolyte multilayer and constitutes the "reaction motor". A seed layer composed of a polyelectrolyte covalently modified by anchoring hydrogelator peptides constitutes the top of the multilayer. This layer is the nucleation site for the Fmoc-FFY peptide self-assembly. When such a film is brought in contact with a Fmoc-FFY(PO4 (2-) ) solution, a nanofiber network starts to form almost instantaneously which extents up to several micrometers into the solution after several hours. We demonstrate that the active seed layer allows convenient control over the self-assembly kinetics and the geometric features of the fiber network simply by changing its peptide density.
authors.affiliation: Institut Charles Sadron, CNRS-UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex (France).
authors.forename: Cécile
authors.initials: C
authors.lastname: Vigier-Carrière
authors.affiliation: Institut Charles Sadron, CNRS-UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex (France).
authors.forename: Tony
authors.initials: T
authors.lastname: Garnier
authors.affiliation: Institut Charles Sadron, CNRS-UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex (France).
authors.forename: Déborah
authors.initials: D
authors.lastname: Wagner
authors.affiliation: INSERM Unité 1121 "Biomaterials and Bioengineering", 11 rue Humann, 67085 Strasbourg Cedex (France).
authors.forename: Philippe
authors.initials: P
authors.lastname: Lavalle
authors.affiliation: INSERM Unité 1121 "Biomaterials and Bioengineering", 11 rue Humann, 67085 Strasbourg Cedex (France).
authors.forename: Morgane
authors.initials: M
authors.lastname: Rabineau
authors.affiliation: INSERM Unité 1121 "Biomaterials and Bioengineering", 11 rue Humann, 67085 Strasbourg Cedex (France).
authors.forename: Joseph
authors.initials: J
authors.lastname: Hemmerlé
authors.affiliation: INSERM Unité 1121 "Biomaterials and Bioengineering", 11 rue Humann, 67085 Strasbourg Cedex (France).
authors.forename: Bernard
authors.initials: B
authors.lastname: Senger
authors.affiliation: Institut Charles Sadron, CNRS-UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex (France). schaaf@unistra.fr.|INSERM Unité 1121 "Biomaterials and Bioengineering", 11 rue Humann, 67085 Strasbourg Cedex (France). schaaf@unistra.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.affiliation: Institut Charles Sadron, CNRS-UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex (France). fouzia.boulmedais@ics-cnrs.unistra.fr.
authors.forename: Fouzia
authors.initials: F
authors.lastname: Boulmedais
authors.affiliation: Institut Charles Sadron, CNRS-UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex (France).
authors.forename: Loïc
authors.initials: L
authors.lastname: Jierry
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D018613:Microscopy, Confocal; D056004:Molecular Dynamics Simulation; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D013501:Surface-Active Agents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D000469:Alkaline Phosphatase
keywords: hydrogels; seed layers; self-assembly; thin layers