title: Boronate-Affinity Glycan-Oriented Surface Imprinting: A New Strategy to Mimic Lectins for the Recognition of an Intact Glycoprotein and Its Characteristic Fragments.
abstract: Lectins possess unique binding properties and are of particular value in molecular recognition. However, lectins suffer from several disadvantages, such as being hard to prepare and showing poor storage stability. Boronate-affinity glycan-oriented surface imprinting was developed as a new strategy for the preparation of lectin-like molecularly imprinted polymers (MIPs). The prepared MIPs could specifically recognize an intact glycoprotein and its characteristic fragments, even within a complex sample matrix. Glycan-imprinted MIPs could thus prove to be powerful tools for important applications such as proteomics, glycomics, and diagnostics.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).
authors.forename: Zijun
authors.initials: Z
authors.lastname: Bie
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).
authors.forename: Jin
authors.initials: J
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).
authors.forename: Shuangshou
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China). zhenliu@nju.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D032701:Biomimetics; D001897:Boronic Acids / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins; D037102:Lectins; D058185:Magnetite Nanoparticles; D011108:Polymers
keywords: boronate affinity; glycans; glycoproteins; molecular imprinting; molecular recognition