title: Alanine-Scanning Mutational Analysis of Durancin GL Reveals Residues Important for Its Antimicrobial Activity.
abstract: Durancin GL is a novel class IIa bacteriocin with 43 residues produced by Enterococcus durans 41D. This bacteriocin demonstrates narrow inhibition spectrum and potent antimicrobial activity against several Listeria monocytogenes strains, including nisin-resistant L. monocytogenes NR30. A systematic alanine-scanning mutational analysis with site-directed mutagenesis was performed to analyze durancin GL residues important for antimicrobial activity and specificity. Results showed that three mutations lost their antimicrobial activity, ten mutations demonstrated a decreased effect on the activity, and seven mutations exhibited relatively high activity. With regard to inhibitory spectrum, four mutants demonstrated a narrower antimicrobial spectrum than wild-type durancin GL. Another four mutants displayed a broader target cell spectrum and increased potency relative to wild-type durancin GL. These findings broaden our understanding of durancin GL residues important for its antimicrobial activity and contribute to future rational design of variants with increased potency.
authors.affiliation: †College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China.
authors.forename: Xingrong
authors.initials: X
authors.lastname: Ju
authors.affiliation: †College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Chen
authors.affiliation: †College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Du
authors.affiliation: †College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China.
authors.forename: Xueyou
authors.initials: X
authors.lastname: Wu
authors.affiliation: ‡Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: †College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
mesh_terms: D000409:Alanine / Q000235:genetics*; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry*; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics*; D004252:DNA Mutational Analysis; D016983:Enterococcus / Q000378:metabolism; D015870:Gene Expression; D008089:Listeria monocytogenes / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D000890:Anti-Infective Agents; D001430:Bacteriocins; D011993:Recombinant Fusion Proteins; C000604181:durancin GL, Enterococcus durans; D000409:Alanine
keywords: Enterococcus; alanine-scanning mutation; antilisteria; durancin GL