title: Comparison of histopathology and PCR based assay for detection of experimentally induced toxoplasmosis in murine model.
abstract: OBJECTIVE
  To compare histopathology and PCR based detection in diagnosis of experimentally induced toxoplasmosis of RH human strain of the parasite in murine models.
  
  
  METHODS
  A comparison of histopathology and PCR based detection was done to diagnose experimentally induced toxoplasmosis in ten inbred swiss albino mice after intraperitoneal inoculation of 100 tachyzoites of laboratory mantained human RH strain of the parasite. Tissue samples from lung, liver, spleen, brain, heart and kidney were taken and processed for histopathological examination while all the samples also were subjected to PCR, using primers directed to the multicopy of SAG 3 gene, in dublicates.
  
  
  RESULTS
  Histopathology revealed presence of tachyzoites only in liver while along with lung, liver, spleen and brain tissue yielded desired positive PCR amplicons.
  
  
  CONCLUSIONS
  The SAG 3 based PCR is able to diagnose toxoplasmosis in those tissues which are declared negative by histopathological assay.
authors.affiliation: Department of Parasitology, College of Veterinary Sciences & Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura 281001, India.
authors.forename: Vikrant
authors.initials: V
authors.lastname: Sudan
authors.affiliation: Department of Parasitology, Indian Veterinary Research Institute, Izatnagar, India. Electronic address: anupktewari@gmail.com.
authors.forename: A K
authors.initials: AK
authors.lastname: Tewari
authors.affiliation: CADRAD, Indian Veterinary Research Institute, Izatnagar, India.
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Parasitology, GADVASU, Ludhiana, India.
authors.forename: Harkirat
authors.initials: H
authors.lastname: Singh
keywords: Experimental infection; Histopathology; SAG 3 based PCR detection; Toxoplasmosis