title: Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis.
abstract: N-terminal acetylation (NTA) catalysed by N-terminal acetyltransferases (Nats) is among the most common protein modifications in eukaryotes, but its significance is still enigmatic. Here we characterize the plant NatA complex and reveal evolutionary conservation of NatA biochemical properties in higher eukaryotes and uncover specific and essential functions of NatA for development, biosynthetic pathways and stress responses in plants. We show that NTA decreases significantly after drought stress, and NatA abundance is rapidly downregulated by the phytohormone abscisic acid. Accordingly, transgenic downregulation of NatA induces the drought stress response and results in strikingly drought resistant plants. Thus, we propose that NTA by the NatA complex acts as a cellular surveillance mechanism during stress and that imprinting of the proteome by NatA is an important switch for the control of metabolism, development and cellular stress responses downstream of abscisic acid.
authors.affiliation: 1] Centre for Organismal Studies, University of Heidelberg, Heidelberg 69120, Germany [2] Hartmut Hoffmann-Berling International Graduate School, University of Heidelberg, Heidelberg 69120, Germany.
authors.forename: Eric
authors.initials: E
authors.lastname: Linster
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, Heidelberg 69120, Germany.
authors.forename: Iwona
authors.initials: I
authors.lastname: Stephan
authors.affiliation: Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 21, 1 avenue de la Terrasse, Gif-sur-Yvette F-91198, France.
authors.forename: Willy V
authors.initials: WV
authors.lastname: Bienvenut
authors.affiliation: Center for Organelle Research, University of Stavanger, Stavanger N-4036, Norway.
authors.forename: Jodi
authors.initials: J
authors.lastname: Maple-Grødem
authors.affiliation: Department of Molecular Biology, University of Bergen, Bergen N-5020, Norway.
authors.forename: Line M
authors.initials: LM
authors.lastname: Myklebust
authors.affiliation: 1] Centre for Organismal Studies, University of Heidelberg, Heidelberg 69120, Germany [2] Hartmut Hoffmann-Berling International Graduate School, University of Heidelberg, Heidelberg 69120, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Huber
authors.affiliation: Max Planck institute for Chemical Ecology, Jena 07745, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Reichelt
authors.affiliation: Center for Medical Research, Mannheim 68167, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Sticht
authors.affiliation: 1] Center for Organelle Research, University of Stavanger, Stavanger N-4036, Norway [2] Department of Biological Sciences, St John's University, New York, New York 11439, USA [3] Norwegian Centre for Movement Disorders, Stavanger University Hospital, Stavanger 4068, Norway.
authors.forename: Simon Geir
authors.initials: SG
authors.lastname: Møller
authors.affiliation: Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 21, 1 avenue de la Terrasse, Gif-sur-Yvette F-91198, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Meinnel
authors.affiliation: 1] Department of Molecular Biology, University of Bergen, Bergen N-5020, Norway [2] Department of Surgery, Haukeland University Hospital, Bergen N-5021, Norway.
authors.forename: Thomas
authors.initials: T
authors.lastname: Arnesen
authors.affiliation: Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 21, 1 avenue de la Terrasse, Gif-sur-Yvette F-91198, France.
authors.forename: Carmela
authors.initials: C
authors.lastname: Giglione
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, Heidelberg 69120, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Hell
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, Heidelberg 69120, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Wirtz
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000107:Acetylation; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D055864:Droughts; D004926:Escherichia coli; D018506:Gene Expression Regulation, Plant; D057809:HEK293 Cells; D006801:Humans; D063209:N-Terminal Acetyltransferase A / Q000235:genetics* / Q000378:metabolism; D030781:Organisms, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D000040:Abscisic Acid; D063209:N-Terminal Acetyltransferase A