title: Phosphatidic acid is a major phospholipid class in reproductive organs of Arabidopsis thaliana.
abstract: Phospholipids are the crucial components of biological membranes and signal transduction. Among different tissues, flower phospholipids are one of the least characterized features of plant lipidome. Here, we report that floral reproductive organs of Arabidopsis thaliana contain high levels of phosphatidic acid (PA), a known lipid second messenger. By using floral homeotic mutants enriched with specific floral organs, lipidomics study showed increased levels of PA species in ap3-3 mutant with enriched pistils. Accompanied gene expression study for 7 diacylglycerol kinases and 11 PA phosphatases revealed distinct floral organ specificity, suggesting an active phosphorylation/dephosphorylation between PA and diacylglycerol in flowers. Our results suggest that PA is a major phospholipid class in floral reproductive organs of A. thaliana.
authors.affiliation: a Institute of Plant and Microbial Biology; Academia Sinica ; Taipei , Taiwan.
authors.forename: Ian Sofian
authors.initials: IS
authors.lastname: Yunus
authors.forename: Amaury
authors.initials: A
authors.lastname: Cazenave-Gassiot
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Liu
authors.forename: Ying-Chen
authors.initials: YC
authors.lastname: Lin
authors.forename: Markus R
authors.initials: MR
authors.lastname: Wenk
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D019852:Diacylglycerol Kinase / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D035264:Flowers / Q000378:metabolism*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000378:metabolism*; D009154:Mutation; D010712:Phosphatidic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation
chemical_list: C101342:APETALA 3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004075:Diglycerides; D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; D010712:Phosphatidic Acids; D010743:Phospholipids; D019852:Diacylglycerol Kinase; D010744:Phosphoric Monoester Hydrolases
keywords: Arabidopsis thaliana; floral reproductive organs; glycerolipids; homeotic mutants; phosphatidic acid