title: Strontium ranelate improves the interaction of osteoblastic cells with titanium substrates: Increase in cell proliferation, differentiation and matrix mineralization.
abstract: We describe direct effects of strontium ranelate on the interaction of osteoblastic cells with different titanium substrates. Our goal was to better understand the potential of this drug for improving the efficacy of bone implants. Treatment was done with 0.12 and 0.5 mM Sr(2+) of strontium ranelate in cell culture. We analyzed cell response to the drug on titanium substrates with surface topographies obtained using acid etching, electro-erosion processing, sandblasting, and machine-tooling. Treatment preserved the initial cell adhesion to the substrates, cell shape parameters (area, aspect ratio, circularity, and solidity), and the orientation of cells on grooved surfaces. However, both concentrations of the drug increased cell proliferation in all substrates. Moreover, a dose-dependent increase in alkaline phosphatase activity and in the production of mineralized matrix with typical features of bone tissue was shown. The observed effects were similar in the different substrates. In conclusion, strontium ranelate improved the interaction of osteoblastic cells with titanium substrates, increasing cell proliferation and differentiation into mature osteoblasts and the production of bone-like mineralized matrix for all substrates. This study highlights a promising role of strontium ranelate on enhancing the clinical success of bone implants, particularly in patients with osteoporosis.
authors.affiliation: a Institut de Sciences des Matériaux de Mulhouse; CNRS UMR7361; Université de Haute-Alsace ; Mulhouse , France.|b Instituto de Ciências Biomédicas; Universidade Federal do Rio de Janeiro ; Rio de Janeiro , Brazil.|c Instituto de Biofísica Carlos Chagas Filho; Universidade Federal do Rio de Janeiro ; Rio de Janeiro , Brazil.
authors.forename: William
authors.initials: W
authors.lastname: Querido
authors.affiliation: b Instituto de Ciências Biomédicas; Universidade Federal do Rio de Janeiro ; Rio de Janeiro , Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Farina
authors.affiliation: a Institut de Sciences des Matériaux de Mulhouse; CNRS UMR7361; Université de Haute-Alsace ; Mulhouse , France.
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D010024:Osteoporosis / Q000188:drug therapy; D013876:Thiophenes / Q000494:pharmacology*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D050071:Bone Density Conservation Agents; D013876:Thiophenes; C081587:strontium ranelate; D014025:Titanium
keywords: biomineralization; cell/material interaction; osteoblastic cells; strontium ranelate; surface topography