title: An Interface Coassembly in Biliquid Phase: Toward Core-Shell Magnetic Mesoporous Silica Microspheres with Tunable Pore Size.
abstract: Core-shell magnetic mesoporous silica microspheres (Magn-MSMs) with tunable large mesopores in the shell are highly desired in biocatalysis, magnetic bioseparation, and enrichment. In this study, a shearing assisted interface coassembly in n-hexane/water biliquid systems is developed to synthesize uniform Magn-MSMs with magnetic core and mesoporous silica shell for an efficient size-selective biocatalysis. The synthesis features the rational control over the electrostatic interaction among cationic surfactant molecules, silicate oligomers, and Fe3O4@RF microspheres (RF: resorcinol formaldehyde) in the presence of shearing-regulated solubilization of n-hexane in surfactant micelles. Through this multicomponent interface coassembly, surfactant-silica mesostructured composite has been uniformly deposited on the Fe3O4@RF microspheres, and core-shell Magn-MSMs are obtained after removing the surfactant and n-hexane. The obtained Magn-MSMs possess excellent water dispersibility, uniform diameter (600 nm), large and tunable perpendicular mesopores (5.0-9.0 nm), high surface area (498-623 m(2)/g), large pore volume (0.91-0.98 cm(3)/g), and high magnetization (34.5-37.1 emu/g). By utilization of their large and open mesopores, Magn-MSMs with a pore size of about 9.0 nm have been demonstrated to be able to immobilize a large bioenzyme (trypsin with size of 4.0 nm) with a high loading capacity of ∼97 μg/mg via chemically binding. Magn-MSMs with immobilized trypsin exhibit an excellent convenient and size selective enzymolysis of low molecular proteins in the mixture of proteins of different sizes and a good recycling performance by using the magnetic separability of the microspheres.
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yue
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Jialuo
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai 201620, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Materials Science and Technology Program, College of Arts and Sciences, Qatar University , PO Box 2713, Doha 2713, Qatar.|Department of Chemistry, College of Science, King Saud University , Riyadh 11451, Saudi Arabia.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Elzatahry
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Xiqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Chemistry, College of Science, King Saud University , Riyadh 11451, Saudi Arabia.
authors.forename: Abdulaziz
authors.initials: A
authors.lastname: Alghamdi
authors.affiliation: Center for Advanced Materials, Qatar University , Doha 2713, Qatar.
authors.forename: Aboubakr M
authors.initials: AM
authors.lastname: Abdullah
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of ASIC & System, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhao
mesh_terms: D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012822:Silicon Dioxide