title: A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish.
abstract: Zebrafish larva is a unique model for whole-brain functional imaging and to study sensory-motor integration in the vertebrate brain. To take full advantage of this system, one needs to design sensory environments that can mimic the complex spatiotemporal stimulus patterns experienced by the animal in natural conditions. We report on a novel open-ended microfluidic device that delivers pulses of chemical stimuli to agarose-restrained larvae with near-millisecond switching rate and unprecedented spatial and concentration accuracy and reproducibility. In combination with two-photon calcium imaging and recordings of tail movements, we found that stimuli of opposite hedonic values induced different circuit activity patterns. Moreover, by precisely controlling the duration of the stimulus (50-500 ms), we found that the probability of generating a gustatory-induced behavior is encoded by the number of neurons activated. This device may open new ways to dissect the neural-circuit principles underlying chemosensory perception.
authors.affiliation: 1] Sorbonne Universités, UPMC Univ. Paris 06, UMR 8237, Laboratoire Jean Perrin, F-75005 Paris, France [2] CNRS UMR 8237, Laboratoire Jean Perrin, F-75005 Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Candelier
authors.affiliation: 1] Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France [2] INSERM, U1024, 75005 Paris, France [3] CNRS, UMR 8197, 75005 Paris, France.
authors.forename: Meena Sriti
authors.initials: MS
authors.lastname: Murmu
authors.affiliation: 1] Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France [2] INSERM, U1024, 75005 Paris, France [3] CNRS, UMR 8197, 75005 Paris, France.
authors.forename: Sebastián Alejo
authors.initials: SA
authors.lastname: Romano
authors.affiliation: 1] Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France [2] INSERM, U1024, 75005 Paris, France [3] CNRS, UMR 8197, 75005 Paris, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Jouary
authors.affiliation: 1] Sorbonne Universités, UPMC Univ. Paris 06, UMR 8237, Laboratoire Jean Perrin, F-75005 Paris, France [2] CNRS UMR 8237, Laboratoire Jean Perrin, F-75005 Paris, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Debrégeas
authors.affiliation: 1] Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France [2] INSERM, U1024, 75005 Paris, France [3] CNRS, UMR 8197, 75005 Paris, France.
authors.forename: Germán
authors.initials: G
authors.lastname: Sumbre
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D056656:Lab-On-A-Chip Devices; D009043:Motor Activity / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000502:physiology; D012212:Rheology; D012903:Smell / Q000502:physiology; D013649:Taste / Q000502:physiology; D015027:Zebrafish / Q000502:physiology*