title: Chemical Composition and Biological Activities of Allium roseum L. var. grandiflorum Briq. Essential Oil.
abstract: Allium roseum L. (Alliaceae) endemic mediterranean specie was represented in the North Africa by 12 different taxa. In the present study, chemical composition, antiproliferative, antioxidant and antimicrobial activities of the essential oil extracted from A. roseum var. grandiflorum Briq. bulbs collected in the North of Tunisia were investigated. Chemical characterization has shown methyl methanethiosulfinate as major sulphurous compounds. A. roseum bulbs essential oil provides interesting antiproliferative activity against two human colonic adenocarcinoma HT29 and CACO2 cell lines in dose-dependent manner with a half-maximal inhibition (IC50) of 4.64 µg/mL and 8.22 µg/mL respectively. The antioxidant activity, as determined by FRAP assay, was 285 µmol equivalent Trolox/g of essential oil. The scavenging effect on DPPH radicals of essential oil was estimated as IC50 values at 156 µg/mL. The inhibition of superoxide anion production in a model of cancer cell lines was significant for both lines HT29 and CACO2 with IC50 of 20.25 µg/mL and 29.12 µg/mL respectively. Allium roseum essential oil exhibited antibacterial and antifungal activities with a high effectiveness against Candida albicans given by an MIC value of 0.019 mg/mL. This biological effect appears to be related mainly to the presence of organosulfur compounds.
authors.affiliation: Laboratoire des substances naturelles (UR10 INRAP 02), Institut National de Recherche et d'Analyse Physico-chimique.
authors.forename: Imen
authors.initials: I
authors.lastname: Touihri
authors.forename: Maher
authors.initials: M
authors.lastname: Boukhris
authors.forename: Naziha
authors.initials: N
authors.lastname: Marrakchi
authors.forename: José
authors.initials: J
authors.lastname: Luis
authors.forename: Belgacem
authors.initials: B
authors.lastname: Hanchi
authors.forename: Olfa
authors.initials: O
authors.lastname: Kallech-Ziri
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000490:Allium / Q000737:chemistry*; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D018938:Caco-2 Cells; D002176:Candida albicans / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D025141:Drug Resistance, Fungal; D016166:Free Radical Scavengers; D019073:HT29 Cells; D006801:Humans; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013457:Sulfur Compounds / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D013457:Sulfur Compounds; D013481:Superoxides