title: Cryopreservation of adhered mammalian cells on a microfluidic device: Toward ready-to-use cell-based experimental platforms.
abstract: In this paper, we describe cryopreservation of mammalian cells in the adhered state on a microfluidic device (microdevice) for the first time. HeLa, NIH3T3, MCF-7, and PC12 cells were cultured on a microdevice in which a commercial polystyrene dish surface was used as the cell adhesion surface. Without cell-detaching treatment, the microdevice was stored in a freezer at -80°C. After thawing, we observed a greater number of live cells on the microdevice than those on a control culture dish. Although the effectiveness of the microdevice varied depending on the cell type and surface coating, the trend was consistent. We confirmed that the phenotype of the PC12 cells to differentiate into neuron-like cells was kept after the on-chip cryopreservation, and that the results of cytotoxicity test of cisplatin against the HeLa cells were essentially unchanged by the on-chip cryopreservation. These findings will open up a new possibility of ready-to-use cell-based experimental platforms.
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.|Department of Materials Science, School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 227-8561, Japan.
authors.forename: Eitaro
authors.initials: E
authors.lastname: Kondo
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Wada
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. k-hoso@riken.go.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hosokawa
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.|Department of Materials Science, School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 227-8561, Japan.
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D015925:Cryopreservation / Q000379:methods*; D004847:Epithelial Cells / Q000502:physiology / Q000528:radiation effects; D005347:Fibroblasts / Q000502:physiology / Q000528:radiation effects; D005615:Freezing; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008322:Mammals; D009474:Neurons / Q000502:physiology / Q000528:radiation effects
keywords: Microfluidic device; adhered cell; cell-based assay; cryopreservation