title: Engineered CHO cells for production of diverse, homogeneous glycoproteins.
abstract: Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
authors.affiliation: 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.affiliation: 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
authors.forename: Adnan
authors.initials: A
authors.lastname: Halim
authors.affiliation: Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Morten Alder
authors.initials: MA
authors.lastname: Schulz
authors.affiliation: Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Frodin
authors.affiliation: 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Shamim H
authors.initials: SH
authors.lastname: Rahman
authors.affiliation: 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Malene B
authors.initials: MB
authors.lastname: Vester-Christensen
authors.affiliation: Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Carsten
authors.initials: C
authors.lastname: Behrens
authors.affiliation: Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Kristensen
authors.affiliation: Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Sergey Y
authors.initials: SY
authors.lastname: Vakhrushev
authors.affiliation: Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Eric Paul
authors.initials: EP
authors.lastname: Bennett
authors.affiliation: Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Hans H
authors.initials: HH
authors.lastname: Wandall
authors.affiliation: 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Clausen
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins