title: Continuous volumetric imaging via an optical phase-locked ultrasound lens.
abstract: In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Kong
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Tang
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Justin P
authors.initials: JP
authors.lastname: Little
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Tim
authors.initials: T
authors.lastname: Lämmermann
authors.affiliation: 1] Center for System Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. [2] Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. [3] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Lin
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Germain
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Cui
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000737:chemistry; D002138:Calibration; D002540:Cerebral Cortex / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007107:Immune System; D007834:Lasers; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D017628:Microglia / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009488:Neurosciences; D009504:Neutrophils / Q000166:cytology; D055095:Optics and Photonics; D009991:Oscillometry
chemical_list: D049452:Green Fluorescent Proteins; D002118:Calcium