title: Tissue-Specific Effects of Esophageal Extracellular Matrix.
abstract: Biologic scaffolds composed of extracellular matrix (ECM) have been used to facilitate repair or remodeling of numerous tissues, including the esophagus. The theoretically ideal scaffold for tissue repair is the ECM derived from the particular tissue to be treated, that is, site-specific or homologous ECM. The preference or potential advantage for the use of site-specific ECM remains unknown in the esophageal location. The objective of the present study was to characterize the in vitro cellular response and in vivo host response to a homologous esophageal ECM (eECM) versus nonhomologous ECMs derived from small intestinal submucosa and urinary bladder. The in vitro response of esophageal stem cells was characterized by migration, proliferation, and three-dimensional (3D) organoid formation assays. The in vivo remodeling response was evaluated in a rat model of esophageal mucosal resection. Results of the study showed that the eECM retains favorable tissue-specific characteristics that enhance the migration of esophageal stem cells and supports the formation of 3D organoids to a greater extent than heterologous ECMs. Implantation of eECM facilitates the remodeling of esophageal mucosa following mucosal resection, but no distinct advantage versus heterologous ECM could be identified.
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Keane
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|3 Department of Pathology, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Aaron
authors.initials: A
authors.lastname: DeWard
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|4 School of Medicine, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Londono
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Lindsey T
authors.initials: LT
authors.lastname: Saldin
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.
authors.forename: Arthur A
authors.initials: AA
authors.lastname: Castleton
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Lisa
authors.initials: L
authors.lastname: Carey
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|5 Hospital Universitario , Fundación Favaloro, Buenos Aires, Argentina .
authors.forename: Alejandro
authors.initials: A
authors.lastname: Nieponice
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|3 Department of Pathology, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Eric
authors.initials: E
authors.lastname: Lagasse
authors.affiliation: 1 McGowan Institute for Regenerative Medicine , Pittsburgh, Pennsylvania.|2 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.|6 Department of Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Badylak
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D004947:Esophagus / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D020100:Hydrogels / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009092:Mucous Membrane / Q000502:physiology; D009928:Organ Specificity; D009940:Organoids / Q000166:cytology / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D020100:Hydrogels; D007633:Keratins