title: The THERMOSENSITIVE MALE STERILE 1 Interacts with the BiPs via DnaJ Domain and Stimulates Their ATPase Enzyme Activities in Arabidopsis.
abstract: The Arabidopsis TMS1 encodes a heat shock protein identical to the Hsp40 protein AtERdj3A and plays important roles in the thermotolerance of pollen tubes and other plant tissues. Despite its importance to plant growth and reproduction, little has been known about its mechanisms underlying thermotolerance of plants. In this study, the relationship between TMS1 and the Hsp70 proteins, Binding Immunoglobulin Proteins (BiPs) was explored to understand the molecular mechanisms of TMS1 in thermotolerance of plants. The expression of TMS1 was induced not only by heat shock, but also by dithiothreitol (DTT) and L-azetidine-2-carboxylic acid (AZC), similarly to the three BiP genes, indicating that TMS1 may be involved in unfolded protein response (UPR). The firefly luciferase complementary imaging (LCI), GST pull-down and ATPase enzyme activity assays demonstrated that the DnaJ domain of TMS1 could interact with BiP1 and BiP3, and could stimulate their ATPase enzyme activities. In addition, the expression level of TMS1 was reduced in the bzip28 bzip60 double mutant. These results suggest that TMS1 may function at the downstream of bZIP28 and bZIP60 and be involved in termotolerance of plants, possibly by participating in refolding or degradation of unfolded and misfolded proteins through interaction with the BiPs.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Leng
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Guang-Xia
authors.initials: GX
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Peng-Min
authors.initials: PM
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China; National Center for Plant Gene Research (Beijing), Beijing, China.
authors.forename: De
authors.initials: D
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Chen
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response / Q000187:drug effects; D049409:Luciferases, Firefly / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C585258:BiP1 protein, Arabidopsis; C585260:BiP3 protein, Arabidopsis; C000596011:ERdj3b protein, Arabidopsis; D050956:HSP40 Heat-Shock Proteins; D018832:Molecular Chaperones; C000607221:THERMOSENSITIVE MALE STERILE 1 protein, Arabidopsis; D001383:Azetidinecarboxylic Acid; D049409:Luciferases, Firefly; D000251:Adenosine Triphosphatases; D004229:Dithiothreitol