title: Magnetic nanoparticles for "smart liposomes".
abstract: Liposomal drug delivery systems (LDDSs) are promising tools used for the treatment of diseases where highly toxic pharmacological agents are administered. Currently, destabilising LDDSs by a specific stimulus at a target site remains a major challenge. The bacterial mechanosensitive channel of large conductance (MscL) presents an excellent candidate biomolecule that could be employed as a remotely controlled pore-forming nanovalve for triggered drug release from LDDSs. In this study, we developed superparamagnetic nanoparticles for activation of the MscL nanovalves by magnetic field. Synthesised CoFe2O4 nanoparticles with the radius less than 10 nm were labelled by SH groups for attachment to MscL. Activation of MscL by magnetic field with the nanoparticles attached was examined by the patch clamp technique showing that the number of activated channels under ramp pressure increased upon application of the magnetic field. In addition, we have not observed any cytotoxicity of the nanoparticles in human cultured cells. Our study suggests the possibility of using magnetic nanoparticles as a specific trigger for activation of MscL nanovalves for drug release in LDDSs.
authors.affiliation: Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Lowy Packer Building, 405 Liverpool St, Darlinghurst, NSW, 2010, Australia.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nakayama
authors.affiliation: Institute for Superconducting and Electronic Material (ISEM), University of Wollongong, Squires Way, North Wollongong, NSW, 2500, Australia.
authors.forename: Mislav
authors.initials: M
authors.lastname: Mustapić
authors.affiliation: Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Lowy Packer Building, 405 Liverpool St, Darlinghurst, NSW, 2010, Australia.
authors.forename: Haleh
authors.initials: H
authors.lastname: Ebrahimian
authors.affiliation: Institute for Superconducting and Electronic Material (ISEM), University of Wollongong, Squires Way, North Wollongong, NSW, 2500, Australia.
authors.forename: Pawel
authors.initials: P
authors.lastname: Wagner
authors.affiliation: Institute for Superconducting and Electronic Material (ISEM), University of Wollongong, Squires Way, North Wollongong, NSW, 2500, Australia.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Institute for Superconducting and Electronic Material (ISEM), University of Wollongong, Squires Way, North Wollongong, NSW, 2500, Australia.
authors.forename: Md Shahriar Al
authors.initials: MS
authors.lastname: Hossain
authors.affiliation: Institute for Superconducting and Electronic Material (ISEM), University of Wollongong, Squires Way, North Wollongong, NSW, 2500, Australia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Horvat
authors.affiliation: Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Lowy Packer Building, 405 Liverpool St, Darlinghurst, NSW, 2010, Australia. B.Martinac@victorchang.edu.au.|St Vincent's Clinical School, University of New South Wales, Darlinghurst, NSW, 2010, Australia. B.Martinac@victorchang.edu.au.
authors.forename: Boris
authors.initials: B
authors.lastname: Martinac
mesh_terms: D045744:Cell Line, Tumor; D003035:Cobalt / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D007473:Ion Channels / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D029968:Escherichia coli Proteins; D005290:Ferric Compounds; D007473:Ion Channels; D008081:Liposomes; D058185:Magnetite Nanoparticles; C086596:MscL protein, E coli; C569492:cobalt ferrite; D003035:Cobalt
keywords: CoFe2O4; Drug delivery system; Liposomes; Magnetic nanoparticles; Mechanosensitive channels; MscL; Patch clamp