title: Snake Cathelicidin NA-CATH and Smaller Helical Antimicrobial Peptides Are Effective against Burkholderia thailandensis.
abstract: Burkholderia thailandensis is a Gram-negative soil bacterium used as a model organism for B. pseudomallei, the causative agent of melioidosis and an organism classified category B priority pathogen and a Tier 1 select agent for its potential use as a biological weapon. Burkholderia species are reportedly "highly resistant" to antimicrobial agents, including cyclic peptide antibiotics, due to multiple resistance systems, a hypothesis we decided to test using antimicrobial (host defense) peptides. In this study, a number of cationic antimicrobial peptides (CAMPs) were tested in vitro against B. thailandensis for both antimicrobial activity and inhibition of biofilm formation. Here, we report that the Chinese cobra (Naja atra) cathelicidin NA-CATH was significantly antimicrobial against B. thailandensis. Additional cathelicidins, including the human cathelicidin LL-37, a sheep cathelicidin SMAP-29, and some smaller ATRA peptide derivatives of NA-CATH were also effective. The D-enantiomer of one small peptide (ATRA-1A) was found to be antimicrobial as well, with EC50 in the range of the L-enantiomer. Our results also demonstrate that human alpha-defensins (HNP-1 & -2) and a short beta-defensin-derived peptide (Peptide 4 of hBD-3) were not bactericidal against B. thailandensis. We also found that the cathelicidin peptides, including LL-37, NA-CATH, and SMAP-29, possessed significant ability to prevent biofilm formation of B. thailandensis. Additionally, we show that LL-37 and its D-enantiomer D-LL-37 can disperse pre-formed biofilms. These results demonstrate that although B. thailandensis is highly resistant to many antibiotics, cyclic peptide antibiotics such as polymyxin B, and defensing peptides, some antimicrobial peptides including the elapid snake cathelicidin NA-CATH exert significant antimicrobial and antibiofilm activity towards B. thailandensis.
authors.affiliation: George Mason University, School of Systems Biology, Manassas, Virginia, United States of America.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Blower
authors.affiliation: George Mason University, School of Systems Biology, Manassas, Virginia, United States of America.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Barksdale
authors.affiliation: George Mason University, School of Systems Biology, Manassas, Virginia, United States of America; George Mason University, National Center for Biodefense and Infectious Diseases, Manassas, Virginia, United States of America.
authors.forename: Monique L
authors.initials: ML
authors.lastname: van Hoek
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D019117:Burkholderia / Q000187:drug effects* / Q000502:physiology; D054804:Cathelicidins / Q000737:chemistry / Q000494:pharmacology*; D017815:Elapidae / Q000378:metabolism; D008969:Molecular Sequence Data; D012756:Sheep
chemical_list: D000900:Anti-Bacterial Agents; D054804:Cathelicidins