title: Bioimaging of transcriptional activity of microRNA124a during neurogenesis.
abstract: OBJECTIVE
  A special vector system was developed to monitor the in vitro and in vivo endogenous level of a primary transcript of miR124a during neuronal differentiation
  
  
  RESULTS
  The upstream regions of miR124a were fused with luciferase (Gluc) and their activity was measured. During neurogenesis of P19 cells, the primary transcript level of miR124a was increased 1.5-times compared to the undifferentiated P19 cells. P19 cells grafted to nude mice exhibited the same pattern of luciferase activity in vivo as they did in vitro.
  
  
  CONCLUSIONS
  The expression of primary miR124a during neurogenesis was successfully imaged by in vitro and in vivo luciferase reporter gene-based method.
authors.affiliation: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Ko
authors.affiliation: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
authors.forename: Jonghwan
authors.initials: J
authors.lastname: Lee
authors.affiliation: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
authors.forename: Yong Seung
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, CHA Unversity, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea.
authors.forename: Youngsok
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Al-Jeraisy DNA Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.|Department of Nucleic Acids Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, 21934, Egypt.
authors.forename: Bahy A
authors.initials: BA
authors.lastname: Ali
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Abdulaziz A
authors.initials: AA
authors.lastname: Al-Khedhairy
authors.affiliation: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea. kimsoonhag@empal.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea. kimsoonhag@empal.com.
authors.forename: Soonhag
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D008969:Molecular Sequence Data; D055495:Neurogenesis / Q000235:genetics* / Q000502:physiology*; D011401:Promoter Regions, Genetic
chemical_list: C509207:MIRN124 microRNA, human; D035683:MicroRNAs
keywords: Bioluminescence imaging; In vivo imaging; MicroRNA124a; Neurogenesis; Promoter