title: [Epidemiological, clinical and biological features of infantile visceral leishmaniasis at Kairouan hospital (Tunisia): about 240 cases].
abstract: Visceral leishmaniasis (VL) is an important health problem in Tunisia. It is most common in children under five years of age. The governorate of Kairouan (central Tunisia) is one of the most affected foci. The aim of this study was to update the epidemiological, clinical and biological features of the disease. The study concerned all VL cases admitted in the pediatric department of Kairouan hospital during 10 years (from 2004 to 2013). For every patient included in this study and when available, data such as sex, age, geographical origin and the condition of the patient at admission (clinical and biological findings) were collected. The myelogram results were also exploited as well as results of serology, culture, Real-Time polymerase chain reaction (PCR) and isoenzymatic typing of Leishmania isolates. Two hundred and forty cases were recorded. Rural cases (87.1%) were more prevalent than urban ones (12.9%). Age ranged from 2 months to 13 years (median, 18 months). The female/male sex ratio was 1.03. The diagnosis delays ranged from 1 day to 8 months (median, 15 days). The most common clinical symptoms at admission were splenomegaly (97.9%), fever (79.9%) and hepatomegaly (47.3%). The principal biological disturbances were anemia (91.7%), thrombocytopenia (83.9%) and leucopenia (56.1%). Among the different biological tools used for diagnosis confirmation, PCR was the most sensitive (100%). All 43 typed stocks corresponded to Leishmania (L.) infantum species. Although zymodeme MON-1 was predictably the most frequent (27 cases), L. infantum MON-24 and MON-80 were responsible of no negligible numbers of cases (11 and 5 cases respectively). The present study gave an updated epidemiological, clinical and biological profile of infantile VL in Tunisia. The diagnosis delays were considerably shortened compared to previous reports. However, an even earlier diagnosis of cases is needed to improve the disease prognosis. Real-Time PCR showed to be helpful in VL management.
authors.affiliation: LR-11-IPT-06 « Parasitoses médicales, biotechnologie et biomolécules », Institut Pasteur de Tunis, 13, place Pasteur, BP 74, 1002, Tunis Belvédère, Tunisie.|Service d'épidémiologie médicale, Institut Pasteur de Tunis, Tunis Belvédère, Tunisie.
authors.forename: W
authors.initials: W
authors.lastname: Aissi
authors.affiliation: Service de pédiatrie, Hôpital Ibn El Jazzar, Kairouan, Tunisie.
authors.forename: K
authors.initials: K
authors.lastname: Ben Hellel
authors.affiliation: LR-11-IPT-06 « Parasitoses médicales, biotechnologie et biomolécules », Institut Pasteur de Tunis, 13, place Pasteur, BP 74, 1002, Tunis Belvédère, Tunisie.|Service de pédiatrie, Hôpital Ibn El Jazzar, Kairouan, Tunisie.
authors.forename: Z
authors.initials: Z
authors.lastname: Habboul
authors.affiliation: LR-11-IPT-06 « Parasitoses médicales, biotechnologie et biomolécules », Institut Pasteur de Tunis, 13, place Pasteur, BP 74, 1002, Tunis Belvédère, Tunisie.
authors.forename: I
authors.initials: I
authors.lastname: Ben Sghaier
authors.affiliation: Laboratoire d'éco-épidémiologie et de génétique des parasites, Institut Pasteur d'Alger, Algiers, Algerie.
authors.forename: Z
authors.initials: Z
authors.lastname: Harrat
authors.affiliation: LR-11-IPT-06 « Parasitoses médicales, biotechnologie et biomolécules », Institut Pasteur de Tunis, 13, place Pasteur, BP 74, 1002, Tunis Belvédère, Tunisie.|Service de parasitologie-mycologie, Institut Pasteur de Tunis, Tunis Belvédère, Tunisie.
authors.forename: A
authors.initials: A
authors.lastname: Bouratbine
authors.affiliation: LR-11-IPT-06 « Parasitoses médicales, biotechnologie et biomolécules », Institut Pasteur de Tunis, 13, place Pasteur, BP 74, 1002, Tunis Belvédère, Tunisie. karim.aoun@pasteur.rns.tn.|Laboratoire d'épidémiologie et d'écologie parasitaire, Institut Pasteur de Tunis, Tunis Belvédère, Tunisie. karim.aoun@pasteur.rns.tn.
authors.forename: K
authors.initials: K
authors.lastname: Aoun
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D057210:Delayed Diagnosis / Q000706:statistics & numerical data; D005260:Female; D006761:Hospitals / Q000706:statistics & numerical data; D006801:Humans; D015994:Incidence; D007223:Infant; D007898:Leishmaniasis, Visceral / Q000453:epidemiology* / Q000473:pathology; D008297:Male; D010343:Patient Admission / Q000706:statistics & numerical data; D014416:Tunisia / Q000453:epidemiology
keywords: Child; Hospital; Kairouan; Leishmania infantum; Maghreb; Northern Africa; PCR; Tunisia; Visceral leishmaniasis