title: Determination of Human Hemoglobin Derivatives.
abstract: The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.
authors.affiliation: a Biophysical Laboratory, Biochemistry Department , National Research Centre , Dokki , Cairo , Egypt.
authors.forename: Atef M M
authors.initials: AM
authors.lastname: Attia
authors.forename: Fatma A A
authors.initials: FA
authors.lastname: Ibrahim
authors.forename: Noha A
authors.initials: NA
authors.lastname: Abd El-Latif
authors.forename: Samir W
authors.initials: SW
authors.lastname: Aziz
authors.forename: Sherif A
authors.initials: SA
authors.lastname: Abdelmottaleb Moussa
authors.forename: Mohsen S
authors.initials: MS
authors.lastname: Elalfy
mesh_terms: D000328:Adult; D002263:Carboxyhemoglobin / Q000737:chemistry / Q000378:metabolism; D005260:Female; D064368:Healthy Volunteers; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008706:Methemoglobin / Q000737:chemistry / Q000378:metabolism; D010108:Oxyhemoglobins / Q000737:chemistry / Q000378:metabolism; D011247:Pregnancy; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins; D008706:Methemoglobin; D002263:Carboxyhemoglobin
keywords: Carboxyhemoglobin (HbCO); hemoglobin (Hb); humans; methemoglobin (metHb); oxyhemoglobin (oxyHb); sulfohemoglobin (SHb)