title: Determination of Acetylation of the Gli Transcription Factors.
abstract: The Gli transcription factors (Gli1, Gli2, and Gli3) are the final effectors of the Hedgehog (Hh) signaling and play a key role in development and cancer. The activity of the Gli proteins is finely regulated by covalent modifications, such as phosphorylation, ubiquitination, and acetylation. Both Gli1 and Gli2 are acetylated at a conserved lysine, and this modification causes the inhibition of their transcriptional activity. Thus, the acetylation status of these proteins represents a useful marker to monitor Hh activation in pathophysiological conditions. Herein we describe the techniques utilized to detect in vitro and intracellular acetylation of the Gli transcription factors.
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, 324 Viale Regina Elena, 00161, Rome, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Coni
authors.forename: Laura
authors.initials: L
authors.lastname: Di Magno
authors.forename: Gianluca
authors.initials: G
authors.lastname: Canettieri
mesh_terms: D000107:Acetylation; D003080:Cold Temperature; D015870:Gene Expression; D057809:HEK293 Cells; D006358:Hot Temperature; D006801:Humans; D047468:Immunoprecipitation; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000071676:Zinc Finger Protein GLI1
chemical_list: D015513:Oncogene Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1