title: Open-top selective plane illumination microscope for conventionally mounted specimens.
abstract: We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips.
authors.forename: Ryan
authors.initials: R
authors.lastname: McGorty
authors.forename: Harrison
authors.initials: H
authors.lastname: Liu
authors.forename: Daichi
authors.initials: D
authors.lastname: Kamiyama
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Dong
authors.forename: Su
authors.initials: S
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation; D000069416:Intravital Microscopy / Q000295:instrumentation*; D008029:Lighting / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation*