title: A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.
abstract: Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described.
authors.affiliation: Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640-00100 Nairobi, Kenya.
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Njoroge
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Boinett
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Liverpool L69 7BE, UK International Livestock Research Institute, PO Box 30709-00100, Nairobi, Kenya.
authors.forename: Laure F
authors.initials: LF
authors.lastname: Madé
authors.affiliation: Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640-00100 Nairobi, Kenya.
authors.forename: Tom T
authors.initials: TT
authors.lastname: Ouko
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Liverpool L69 7BE, UK International Livestock Research Institute, PO Box 30709-00100, Nairobi, Kenya.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Fèvre
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Thomson
authors.affiliation: Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640-00100 Nairobi, Kenya Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK samkariuki2@gmail.com.
authors.forename: Samuel
authors.initials: S
authors.lastname: Kariuki
mesh_terms: D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007630:Kenya; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D000954:Antigens, Surface; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins
keywords: Enterotoxigenic Escherichia coli; Kenya; coli surface antigen 8 subtype B; colonization factors; type IVb pilins