title: Use of polymeric membranes for purification of an E. coli expressed biotherapeutic protein.
abstract: Polymers have had a significant impact on the field of bioseparations in the past few decades. Most recently, membrane chromatography has emerged as an efficient alternative to the conventional packed-bed chromatography by eliminating the diffusion-related limitations associated with the traditional resin beads. In this article, we examine six membrane adsorbers for purification of granulocyte colony-stimulating factor (GCSF), an Escherichia coli-based biotherapeutic. These adsorbers differ either in their base matrix or in the surface chemistry. The role of interactions between the filter surfaces and the protein molecules in effecting these separations is the focus of the article.
authors.affiliation: a Department of Chemical Engineering , Indian Institute of Technology , Hauz Khas , New Delhi , India.
authors.forename: S
authors.initials: S
authors.lastname: Muthukumar
authors.affiliation: a Department of Chemical Engineering , Indian Institute of Technology , Hauz Khas , New Delhi , India.
authors.forename: Anurag S
authors.initials: AS
authors.lastname: Rathore
mesh_terms: D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics*; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor
keywords: Diffusion; granulocyte colony-stimulating factor (GCSF); host cell protein; membrane chromatography; surface chemistry