title: In Cell Interactome of Oleocanthal, an Extra Virgin Olive Oil Bioactive Component.
abstract: A copper-(I)-catalyzed variation of the Huisgen 1,3-dipolar cycloaddition has been applied to lead the in living-cell mass-spectrometry based identification of protein targets of oleocanthal, a natural metabolite daily ingested by millions of people. Chemical proteomics revealed heat-shock proteins, HSP70 and HSP90, as main oleocanthal interactors in living systems. These two proteins are involved in cancer development and, thus, our findings could have important outcomes for a deep evaluation of the bio-pharmacological significance of oleocanthal.
authors.forename: Chiara
authors.initials: C
authors.lastname: Cassiano
authors.forename: Agostino
authors.initials: A
authors.lastname: Casapullo
authors.forename: Alessandra
authors.initials: A
authors.lastname: Tosco
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Monti
authors.forename: Raffaele
authors.initials: R
authors.lastname: Riccio
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D000081004:Cyclopentane Monoterpenes; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D000069463:Olive Oil; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics
chemical_list: D000447:Aldehydes; D000081004:Cyclopentane Monoterpenes; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; C503534:oleocanthal