title: [Solasonine-induced Apoptosis in Lung Cancer Cell Line H446 and Its Mechanism].
abstract: OBJECTIVE
  Incidence and mortality rates of lung cancer are rising sharply. Small cell lung cancer patients prefer chemotherapy than surgery because of the insignificant side effects of Chinese medicine. Studies have shown that solasonine possesses an anti-tumor property. The aim of this study is to investigate the effect of solasonine on the apoptosis of lung cancer cell line H446.
  
  
  METHODS
  Appropriate concentration and time were selected with a CCK8 kit. The drug that was used in H446 cells was divided into four different concentrations: 0 μmol/L, 3.4 μmol/L, 6.8 μmol/L and 13.6 μmol/L. The changes in forms and nucleus of H446 cells that were stained with DAPI were observed under an inverted optical microscope. The effects on H446 cell apoptosis were detected by FCM. The changes in apoptosis-related proteins BCL2, BAX, and CASP3 were investigated using Western blot for 24 h.
  
  
  RESULTS
  Solasonine reduced the survival ratio of H446 cells and inhibited the proliferation with a dose-related effect. The survival ratio of H446 cells could be reduced to 16.77% (P<0.001), and the highest apoptosis ratio was 44.62% (P<0.001). Apoptosis was observed in H446 cells. Moreover, Western blot showed that the apoptosis-related proteins BAX and CASP3 were upregulated (P<0.05).
  
  
  CONCLUSIONS
  The proliferation of H446 cells can be inhibited by solasonine, and the expression of pro-apoptotic proteins is up-regulated, and the expression of anti-apoptotic proteins is down-regulated, thereby promoting the apoptosis of cells.
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Wensi
authors.initials: W
authors.lastname: Huang
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xie
authors.affiliation: Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012991:Solanaceous Alkaloids / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D012991:Solanaceous Alkaloids; C026608:alpha-solamargine; D053148:Caspase 3