title: Inhibitory effect of midkine-binding peptide on tumor proliferation and migration.
abstract: BACKGROUND
  To investigate the inhibitory effect of midkine-binding peptides on human umbilical vein endothelial cells (HUVECs) proliferation and angiogenesis of xenograft tumor.
  
  
  METHODS
  The midkine-binding peptides were panned by Ph.D.-7(™) Phage Display Peptide Library Kit, and the specific binding activities of positive clones to target protein were examined by phage ELISA. The effect of midkine-binding peptides on proliferation of HUVECs was confirmed by MTT test. The xenograft tumor model was formed in BALB/c mice with the murine hepatocarcinoma cells H22 (H22). Microvessel density (MVD) was analyzed by immunohistochemistry of factor VIII staining.
  
  
  RESULTS
  Midkine-binding peptides have the inhibitory effects on tumor angiogenesis, a proliferation assay using human umbilical vein endothelial cells (HUVECs) indicated that particular midkine-binding peptides significantly inhibited the proliferation of the HUVECs. Midkine-binding peptides were also observed to efficiently suppress angiogenesis induced by murine hepatocarcinoma H22 cells in BALB/c nude mice.
  
  
  CONCLUSIONS
  The midkine-binding peptides can inhibit solid tumor growth by retarding the formation of new blood vessels. The results indicate that midkine-binding peptides may represent potent anti-angiogenesis agents in vivo.
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou 313000, China.
authors.forename: Hui-Lian
authors.initials: HL
authors.lastname: Huang
authors.affiliation: The First Hospital of Jiaxing Jiaxing 314000, China.
authors.forename: Jian-Fen
authors.initials: JF
authors.lastname: Shen
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou 313000, China.
authors.forename: Li-Shan
authors.initials: LS
authors.lastname: Min
authors.affiliation: Department of Pathology, Huzhou Central Hospital Huzhou 313000, Zhejiang Province, China.
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Ping
authors.affiliation: Huzhou Teacher's Collage Huzhou 31300, Zhejiang Province, China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Lu
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital Huzhou 313000, China.
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Dai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D016207:Cytokines / Q000037:antagonists & inhibitors*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D000078792:Midkine; D009389:Neovascularization, Pathologic / Q000473:pathology; D010455:Peptides / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D016207:Cytokines; D010455:Peptides; D000078792:Midkine
keywords: Midkine; angiogenesis; peptide