title: Protective effect of Enterococcus faecalis DAPTO 512 on the intestinal tract and gut mucosa: milk allergy application.
abstract: The allergenicity of β-lactoglobulin (β-Lg) was studied by using Ussing chamber in a murine model of β-Lg allergy supplemented with hydrolysates obtained after fermentation of milk for 48 h at 37 (°)C with Enterococcus faecalis DAPTO 512, isolated from cow milk and identified by 16S rDNA sequence analysis. Balb/c mice were sensitised intraperitoneally with β-Lg. Three groups of mice were formed: group 1, composed of naive mice used as control received only NaCl; group 2, positive control composed of mice sensitised intraperitoneally with β-Lg; group 3, formed by mice which were given hydrolysates of 48 h then sensitised with β-Lg. After 48 h of fermentation β-casein and β-Lg were degraded by E. faecalis DAPTO 512. β-Lg immunisation was associated with strong IgG and IgE production in case of positive controls and a significant increase in short current circuit (Isc) and high conductance (G) responses were observed. The control and the hydrolysate groups showed a significant decrease in the production of IgG and IgE anti β-Lg compared to the positive control. The allergenic potential of β-Lg was markedly reduced in the group that received hydrolysates (Isc and G remained unchanged after intestine challenge with β-Lg). The histological scrutiny showed villi atrophy, lymphocyte hyperplasia and a significant chorion detachment in the positive control group. In the group administered with hydrolysates of fermented milk, inflammatory signs were lower, the villi were long and thin and lymphocytes were less dense. The results showed that feeding of milk fermented with E. faecalis DAPTO 512 during 18 days prior to β-Lg allergy induction exerts a protecting effect on the murine intestine and induces a significant decrease in the β-Lg allergenicity.
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: K
authors.initials: K
authors.lastname: Belkaaloul
authors.affiliation: 2 UR 1268, Biopolymères Interactions Assemblages, INRA, BP 71627, 44300 Nantes, France.
authors.forename: T
authors.initials: T
authors.lastname: Haertlé
authors.affiliation: 2 UR 1268, Biopolymères Interactions Assemblages, INRA, BP 71627, 44300 Nantes, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Chobert
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: R
authors.initials: R
authors.lastname: Merah
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: K
authors.initials: K
authors.lastname: Taibi
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: H A
authors.initials: HA
authors.lastname: Saad El Hachemi
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: S
authors.initials: S
authors.lastname: Hemch
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: L
authors.initials: L
authors.lastname: Amier
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Chekroun
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: D
authors.initials: D
authors.lastname: Saidi
authors.affiliation: 1 Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Science and Life, University of Oran, P.O. Box 1524, El m'naouer Oran 31000, Algeria.
authors.forename: O
authors.initials: O
authors.lastname: Kheroua
mesh_terms: D000485:Allergens / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D013293:Enterococcus faecalis / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000473:pathology*; D007782:Lactoglobulins / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008853:Microscopy; D016269:Milk Hypersensitivity / Q000473:pathology / Q000517:prevention & control*; D059748:Proteolysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000485:Allergens; D002364:Caseins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007782:Lactoglobulins; D012336:RNA, Ribosomal, 16S
keywords: Enterococcus; allergy disease; casein; intestine; β-Lg