title: Inhibition of human neutrophil elastase by labdane diterpenes from the fruiting bodies of Ramaria formosa.
abstract: Two new labdane diterpenes (1 and 2) were isolated from the fruiting bodies of Ramaria formosa. The structures of these compounds were established by extensive spectroscopic studies and chemical evidence. The inhibitory activity of compounds 1 and 2 against human neutrophil elastase (HNE) was evaluated in vitro. Compounds 1 and 2 inhibited HNE activity moderately. The IC50 values for compounds 1 and 2 were 36.4 ± 1.2 and 40.8 ± 1.5 μM, respectively; the IC50 value for the positive control, EGCG, was 12.5 ± 0.8 μM. In addition, the mechanism by which 2 inhibited HNE was a mixed-type noncompetitive inhibition, with a Ki of 41.5 ± 1.8 μM.
authors.affiliation: a KM Convergence Research Division , Korea Institute of Oriental Medicine , Daejeon , Republic of Korea.
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Lee
authors.affiliation: b Department of Public Health , Graduate School of Science, Eulji University , Seongnam , Republic of Korea.|c Korea Research Institute of Bioscience and Biotechnology , Innoskin Co. , Daejeon , Republic of Korea.
authors.forename: Kwan-Chul
authors.initials: KC
authors.lastname: Kim
authors.affiliation: c Korea Research Institute of Bioscience and Biotechnology , Innoskin Co. , Daejeon , Republic of Korea.
authors.forename: Ick-Dong
authors.initials: ID
authors.lastname: Yoo
authors.affiliation: b Department of Public Health , Graduate School of Science, Eulji University , Seongnam , Republic of Korea.
authors.forename: Byung-Jo
authors.initials: BJ
authors.lastname: Ha
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004224:Diterpenes; D011480:Protease Inhibitors; D019272:Leukocyte Elastase
keywords: Gomphaceae; Ramaria formosa; human neutrophil elastase; labdane diterpene