title: Proinflammatory interleukins' production by adipose tissue-derived mesenchymal stromal cells: the impact of cell culture conditions and cell-to-cell interaction.
abstract: The impact of culture conditions and interaction with activated peripheral blood mononuclear cells on the interleukin (IL) gene expression profile and proinflammatory IL-6 and IL-8 production by adipose-derived stromal cells (ASCs) was investigated. A microarray analysis revealed a wide range of IL genes either under standard (20%) or hypoxic (5%) O2 concentrations, some highly up-regulated at hypoxia. IL-6 and IL-8 production was inversely dependent on cell culture density. In early (first-third) passages, IL-6 and IL-8 concentration was higher at 20% O2 and in late (8th-12th) passages under 5% O2. Interaction between ASCs and mononuclear cells in indirect setting was accompanied with a significant decrease of IL-6 and did not result in the elevation of IL-8 concentration. Thereby, the production of proinflammatory interleukins (IL-6 and IL-8) may be affected by the ASC intrinsic features (density in culture, and duration of expansion), as well as by microenvironmental factors, such as hypoxia and the presence of blood-borne cells. These data are important for elucidating ASC paracrine activity regulation in vitro. They would also be on demand for optimisation of the cell therapy protocols, based on the application of ASC biologically active substances. SIGNIFICANCE PARAGRAPH: Ex vivo expansion is widely used for increasing the number of adipose-derived stromal cells (ASCs) and improving of their quality. The present study was designed to elucidate the particular factors influencing the interleukin production in ASCs. The presented data specified the parameters (i.e. cell density, duration of cultivation, hypoxia, etc.) that should be taken in mind when ASCs are intended to be used in protocols implying their paracrine activity. These data would be of considerable interest for researchers and clinicians working in the biomedical science.
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Andreeva
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.|Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Andrianova
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Julia
authors.initials: J
authors.lastname: Rylova
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Gornostaeva
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Polina
authors.initials: P
authors.lastname: Bobyleva
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Buravkova
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D010100:Oxygen
keywords: cell-to-cell interaction; human adipose tissue-derived stromal cells; hypoxia; interleukins; mononuclear cells from human peripheral blood