title: Characterization of Receptor Binding Profiles of Influenza A Viruses Using An Ellipsometry-Based Label-Free Glycan Microarray Assay Platform.
abstract: A key step leading to influenza viral infection is the highly specific binding of a viral spike protein, hemagglutinin (HA), with an extracellular glycan receptor of a host cell. Detailed and timely characterization of virus-receptor binding profiles may be used to evaluate and track the pandemic potential of an influenza virus strain. We demonstrate a label-free glycan microarray assay platform for acquiring influenza virus binding profiles against a wide variety of glycan receptors. By immobilizing biotinylated receptors on a streptavidin-functionalized solid surface, we measured binding curves of five influenza A virus strains with 24 glycans of diverse structures and used the apparent equilibrium dissociation constants (avidity constants, 10-100 pM) as characterizing parameters of viral receptor profiles. Furthermore by measuring binding kinetic constants of solution-phase glycans to immobilized viruses, we confirmed that the glycan-HA affinity constant is in the range of 10 mM and the reaction is enthalpy-driven.
authors.affiliation: Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Fudan University, 220 Handan Road, Shanghai 200433, China. fyy@fudan.edu.cn.|Department of Physics, University of California, Davis, CA 95616, USA. fyy@fudan.edu.cn.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Fei
authors.affiliation: Department of Physics, University of California, Davis, CA 95616, USA. bacan1125@gmail.com.|Department of Physics, Fu-Jen Catholic University, New Taipei City 24205, Taiwan. bacan1125@gmail.com.
authors.forename: Yung-Shin
authors.initials: YS
authors.lastname: Sun
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616, USA. yhzli@ucdavis.edu.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616, USA. hyu@ucdavis.edu.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616, USA. edlau@ucdavis.edu.
authors.forename: Kam
authors.initials: K
authors.lastname: Lau
authors.affiliation: Department of Physics, University of California, Davis, CA 95616, USA. jim.landry@gmail.com.
authors.forename: James P
authors.initials: JP
authors.lastname: Landry
authors.affiliation: Center for Comparative Medicine, University of California, Davis, CA 95616, USA. zluo@ucdavis.edu.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Center for Comparative Medicine, University of California, Davis, CA 95616, USA. nbaumgarth@ucdavis.edu.
authors.forename: Nicole
authors.initials: N
authors.lastname: Baumgarth
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616, USA. chen@chem.ucdavis.edu.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Physics, University of California, Davis, CA 95616, USA. xdzhu@physics.ucdavis.edu.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000378:metabolism; D009980:Influenza A virus / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D055096:Optical Devices; D011134:Polysaccharides / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D008024:Ligands; D011134:Polysaccharides; D011991:Receptors, Virus
keywords: binding profile; biosensors; ellipsometry; glycans; high-throughput; influenza A virus; label-free; microarray; reaction kinetics