title: Large scale systematic proteomic quantification from non-metastatic to metastatic colorectal cancer.
abstract: A systematic proteomic quantification of formalin-fixed, paraffin-embedded (FFPE) colorectal cancer tissues from stage I to stage IIIC was performed in large scale. 1017 proteins were identified with 338 proteins in quantitative changes by label free method, while 341 proteins were quantified with significant expression changes among 6294 proteins by iTRAQ method. We found that proteins related to migration expression increased and those for binding and adherent decreased during the colorectal cancer development according to the gene ontology (GO) annotation and ingenuity pathway analysis (IPA). The integrin alpha 5 (ITA5) in integrin family was focused, which was consistent with the metastasis related pathway. The expression level of ITA5 decreased in metastasis tissues and the result has been further verified by Western blotting. Another two cell migration related proteins vitronectin (VTN) and actin-related protein (ARP3) were also proved to be up-regulated by both mass spectrometry (MS) based quantification results and Western blotting. Up to now, our result shows one of the largest dataset in colorectal cancer proteomics research. Our strategy reveals a disease driven omics-pattern for the metastasis colorectal cancer.
authors.affiliation: 1] Department of Systems Biology for Medicine, Fudan University Shanghai Medical College, Shanaghai 200032, China [2] Department of Chemistry and Institutes of Biomedical Sciences of Shanghai medical School, Fudan University, Shanghai 200032, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences of Shanghai medical School, Fudan University, Shanghai 200032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Shaowen
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences of Shanghai medical School, Fudan University, Shanghai 200032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.affiliation: Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1] Department of Systems Biology for Medicine, Fudan University Shanghai Medical College, Shanaghai 200032, China [2] Department of Chemistry and Institutes of Biomedical Sciences of Shanghai medical School, Fudan University, Shanghai 200032, China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D051378:Actin-Related Protein 3 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D002465:Cell Movement; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D016208:Databases, Factual; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D039564:Integrin alphaV / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009362:Neoplasm Metastasis; D016612:Paraffin Embedding; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D019096:Vitronectin / Q000378:metabolism
chemical_list: D051378:Actin-Related Protein 3; D014408:Biomarkers, Tumor; D039564:Integrin alphaV; D020543:Proteome; D019096:Vitronectin