title: Gel mesh as "brake" to slow down DNA translocation through solid-state nanopores.
abstract: Agarose gel is introduced onto the cis side of silicon nitride nanopores by a simple and low-cost method to slow down the speed of DNA translocation. DNA translocation speed is slowed by roughly an order of magnitude without losing signal to noise ratio for different DNA lengths and applied voltages in gel-meshed nanopores. The existence of the gel moves the center-of-mass position of the DNA conformation further from the nanopore center, contributing to the observed slowing of translocation speed. A reduced velocity fluctuation is also noted, which is beneficial for further applications of gel-meshed nanopores. The reptation model is considered in simulation and agrees well with the experimental results.
authors.affiliation: State Key Laboratory for Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, P. R. China. zhaoqing@pku.edu.cn yudp@pku.edu.cn.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Tang
authors.forename: Zexi
authors.initials: Z
authors.lastname: Liang
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Dapeng
authors.initials: D
authors.lastname: Yu
mesh_terms: D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D005782:Gels / Q000737:chemistry; D008956:Models, Chemical; D009038:Motion; D058608:Nanopores; D012685:Sepharose / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry*
chemical_list: D005782:Gels; D017655:Silicon Compounds; D004247:DNA; D012685:Sepharose; C032734:silicon nitride