title: Thermostable Carbonic Anhydrases in Biotechnological Applications.
abstract: Carbonic anhydrases are ubiquitous metallo-enzymes which catalyze the reversible hydration of carbon dioxide in bicarbonate ions and protons. Recent years have seen an increasing interest in the utilization of these enzymes in CO2 capture and storage processes. However, since this use is greatly limited by the harsh conditions required in these processes, the employment of thermostable enzymes, both those isolated by thermophilic organisms and those obtained by protein engineering techniques, represents an interesting possibility. In this review we will provide an extensive description of the thermostable carbonic anhydrases so far reported and the main processes in which these enzymes have found an application.
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. anna.difiore@cnr.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Di Fiore
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. vincenzo.alterio@cnr.it.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Alterio
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. marmonti@unina.it.
authors.forename: Simona M
authors.initials: SM
authors.lastname: Monti
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. gdesimon@unina.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: De Simone
authors.affiliation: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. katia.dambrosio@cnr.it.
authors.forename: Katia
authors.initials: K
authors.lastname: D'Ambrosio
mesh_terms: D001419:Bacteria / Q000201:enzymology; D002119:Calcium Carbonate / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D015202:Protein Engineering; D017434:Protein Structure, Tertiary
chemical_list: D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D002119:Calcium Carbonate
keywords: CO2 capture process; carbonic anhydrases; protein engineering; thermostable enzyme