title: Downregulated microRNA-155 expression in peripheral blood mononuclear cells of type 2 diabetic patients is not correlated with increased inflammatory cytokine production.
abstract: OBJECTIVE
  The role of miR-155 in immune responses of PBMCs of type 2 diabetic (T2D) patients has not been studied.
  
  
  METHODS
  20 Healthy and 20 T2D subjects were participated in the study. miR-155 expression in PBMCs of the subjects was measured using real-time PCR. The levels of secreted IL-6 and TNF-α cytokines were quantified using ELISA.
  
  
  RESULTS
  A downregulation of miR-155 expression was observed in untreated and LPS treated PBMCs of diabetic patients compared to controls. There was a significant upregulation of miR-155 after LPS treatment in PBMCs of both control and diabetic groups. In healthy subjects and in both untreated and LPS-treated conditions, miR-155 expression was negatively correlated with weight, waist circumference and body mass index. In diabetic group, there was a negative correlation between miR-155 expression and glucose levels only in LPS treated cells. Furthermore, systolic blood pressure was found to negatively correlate with miR-155 expression in untreated PBMCs of both healthy and diabetic subjects. The results also showed a significant correlation between miR-155 expression and TNF-α and IL-6 levels in LPS treated cells.
  
  
  CONCLUSIONS
  Our data demonstrate that miR-155 expression is reduced in PBMCs of diabetic patients and this reduced expression does not seem to be involved in increased cytokine production from PBMCs of these patients.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mazloom
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Alizadeh
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran; Diabetes Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Parvin
authors.initials: P
authors.lastname: Pasalar
authors.affiliation: Diabetes Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran; Endocrinology & Metabolism Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Ensieh Nasli
authors.initials: EN
authors.lastname: Esfahani
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran. Electronic address: rmeshkani@tums.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Meshkani
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; C524942:MIRN155 microRNA, human; D035683:MicroRNAs
keywords: Cytokine; Diabetes; Inflammation; Peripheral blood mononuclear cells; miR-155