title: Rab GTPases are essential for membrane trafficking-dependent growth and pathogenicity in Fusarium graminearum.
abstract: Rab GTPases represent the largest subfamily of Ras-related small GTPases and regulate membrane trafficking. Vesicular transport is a general mechanism that governs intracellular membrane trafficking along the endocytic and exocytic pathways in all eukaryotic cells. Fusarium graminearum is a filamentous fungus and causes the devastating and economically important head blight of wheat and related species. The mechanism of vesicular transport is not well understood, and little is known about Rab GTPases in F. graminearum. In this study, we systematically characterized all eleven FgRabs by live cell imaging and genetic analysis. We find that FgRab51 and FgRab52 are important for the endocytosis, FgRab7 localizes to the vacuolar membrane and regulates the fusion of vacuoles and autophagosomes, and FgRab8 and FgRab11 are important for polarized growth and/or exocytosis. Furthermore, both endocytic and exocytic FgRabs are required for vegetative growth, conidiogenesis, sexual reproduction, as well as pathogenesis and deoxynivalenol metabolism in F. graminearum. Thus, we conclude that Rab GTPases are essential for membrane trafficking-dependent growth and pathogenicity in F. graminearum.
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Congxian
authors.initials: C
authors.lastname: Wu
authors.affiliation: Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Deng
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Lu
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.|Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Guangpu
authors.initials: G
authors.lastname: Li
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.|Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ebbole
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D005089:Exocytosis / Q000235:genetics* / Q000502:physiology; D005670:Fusarium / Q000235:genetics / Q000472:pathogenicity*; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism; D014255:Trichothecenes / Q000378:metabolism; D014908:Triticum / Q000382:microbiology; D014774:Virulence; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014255:Trichothecenes; D020691:rab GTP-Binding Proteins; C007262:deoxynivalenol