title: Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus.
abstract: Rapid amplification of cDNA ends (RACE) is a technique that was developed to swiftly and efficiently amplify full-length RNA molecules in which the terminal ends have not been characterized. Current usage of this procedure has been more focused on sequencing and characterizing RNA 5' and 3' untranslated regions. Herein is described an adapted RACE protocol to amplify bacterial RNA transcripts.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of Nevada Reno, 1664 N. Virginia Street, Reno, NV, 89557, USA. ericmiller@medicine.nevada.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Miller
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*; D013210:Staphylococcus / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D012329:RNA, Bacterial
keywords: Amplification of cDNA ends; RNA mapping; Transcriptional start site; cDNA amplification; mRNA sequencing