title: Validation of an analytical method for human plasma free amino acids by high-performance liquid chromatography ionization mass spectrometry using automated precolumn derivatization.
abstract: The analysis of human plasma free amino acids is important for diagnosing the health of individuals, because their concentrations are known to vary with various diseases. The development of valid, reliable, and high-throughput analytical methods for amino acids analysis is an essential requirement in clinical applications. In the present study, we have developed an automated precolumn derivatization amino acid analytical method based on high-performance liquid chromatography/electrospray ionization mass spectrometry (so-called UF-Amino Station). This method enabled the separation of at least 38 types of physiological amino acids within 8min, and the interval time between injections was 12min. We also validated this method for 21 major types of free amino acids in human plasma samples. The results of the specificity, linearity, accuracy, repeatability, intermediate precision, reproducibility, limits of detections, lower limits of quantification, carry over, and sample solution stability were sufficient to allow for the measurement of amino acids in human plasma samples. Our developed method should be suitable for use in clinical fields.
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: kazuhiro_kondo@ajinomoto.com.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kondo
authors.affiliation: Frontier Research Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: h.yama2396@gmail.com.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: naoko_kageyama@ajinomoto.com.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kageyama
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: shinichi_ozawa@ajinomoto.com.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Ozawa
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: kazutaka_shinbo@ajinomoto.com.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Shimbo
authors.affiliation: Frontier Research Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: takahiko_muramatsu@ajinomoto.com.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Muramatsu
authors.affiliation: Frontier Research Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: akira_imaizumi@ajinomoto.com.
authors.forename: Akira
authors.initials: A
authors.lastname: Imaizumi
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: toshimi_mizukoshi@ajinomoto.com.
authors.forename: Toshimi
authors.initials: T
authors.lastname: Mizukoshi
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Junichi
authors.initials: J
authors.lastname: Masuda
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakayama
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayakawa
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Mukaibatake
authors.affiliation: Fundamental Technology Laboratories, Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. Electronic address: hiroo_yoshida@ajinomoto.com.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyano
mesh_terms: D000596:Amino Acids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D000596:Amino Acids
keywords: Automated precolumn derivatization; LC–MS; Plasma free amino acid analysis; UF-Amino Station; Validation