title: Delayed adverse effects of neonatal exposure to polymeric nanoparticle poly(ethylene glycol)-block-polylactide methyl ether on hypothalamic-pituitary-ovarian axis development and function in Wistar rats.
abstract: We studied delayed effects of neonatal exposure to polymeric nanoparticle poly(ethylene glycol)-block-polylactide methyl ether (PEG-b-PLA) on the endpoints related to pubertal development and reproductive function in female Wistar rats from postnatal day 4 (PND4) to PND 176. Female pups were injected intraperitoneally, daily, from PND4 to PND7 with PEG-b-PLA (20 or 40mg/kg b.w.). Both doses of PEG-b-PLA accelerated the onset of vaginal opening compared with the control group. In the low-dose PEG-b-PLA-treated group, a significantly reduced number of regular estrous cycles, increased pituitary weight due to hyperemia, vascular dilatation and congestion, altered course of hypothalamic gonadotropin-releasing hormone-stimulated luteinizing hormone secretion, and increased progesterone serum levels were observed. The obtained data indicate that neonatal exposure to PEG-b-PLA might affect the development and function of hypothalamic-pituitary-ovarian axis (HPO), and thereby alter functions of the reproductive system in adult female rats. Our study indicates a possible neuroendocrine disrupting effect of PEG-b-PLA nanoparticles.
authors.affiliation: Slovak Medical University Bratislava, Department of Toxicology, Bratislava, Slovak Republic. Electronic address: eva.rollerova@szu.sk.
authors.forename: Eva
authors.initials: E
authors.lastname: Rollerova
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: jana.jurcovicova@savba.sk.
authors.forename: Jana
authors.initials: J
authors.lastname: Jurcovicova
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: alzbeta.mlynarcikova@savba.sk.
authors.forename: Alzbeta
authors.initials: A
authors.lastname: Mlynarcikova
authors.affiliation: Hameln rds a.s., Department of Toxicology, Modra, Slovak Republic. Electronic address: I.Sadlonova@hameln-rds.com.
authors.forename: Irina
authors.initials: I
authors.lastname: Sadlonova
authors.affiliation: Qi Technologies s.r.l., Pomezia, Italy. Electronic address: dagmarbilanicova@gmail.com.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Bilanicova
authors.affiliation: Slovak Medical University Bratislava, Department of Toxicology, Bratislava, Slovak Republic. Electronic address: ladislava.wsolova@szu.sk.
authors.forename: Ladislava
authors.initials: L
authors.lastname: Wsolova
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: alexander.kiss@savba.sk.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kiss
authors.affiliation: Slovak Medical University Bratislava, Department of Toxicology, Bratislava, Slovak Republic. Electronic address: jevgenij.kovriznych@szu.sk.
authors.forename: Jevgenij
authors.initials: J
authors.lastname: Kovriznych
authors.affiliation: Institute of Polymers, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: juraj.kronek@savba.sk.
authors.forename: Juraj
authors.initials: J
authors.lastname: Kronek
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: Fedor.Ciampor@savba.sk.
authors.forename: Fedor
authors.initials: F
authors.lastname: Ciampor
authors.affiliation: Institute of Electrical Engineering, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: Ivo.Vavra@savba.sk.
authors.forename: Ivo
authors.initials: I
authors.lastname: Vavra
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: sona.scsukova@hotmail.com.
authors.forename: Sona
authors.initials: S
authors.lastname: Scsukova
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D007773:Lactates / Q000633:toxicity*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D053758:Nanoparticles / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary; D010902:Pituitary Gland / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D010913:Pituitary-Adrenal System / Q000187:drug effects; D011092:Polyethylene Glycols / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011374:Progesterone / Q000097:blood; D017208:Rats, Wistar
chemical_list: D007773:Lactates; C088513:poly(lactic acid-ethylene glycol); D007987:Gonadotropin-Releasing Hormone; D011092:Polyethylene Glycols; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Endocrine disruptors; Estrous cycle; Nanotoxicology; PEG-b-PLA; Pituitary; Polymeric nanoparticles; Progesterone; Reproductive toxicity