title: Regulation of hepatic lipase activity by sphingomyelin in plasma lipoproteins.
abstract: Hepatic lipase (HL) is an important enzyme in the clearance of triacylglycerol (TAG) from the circulation, and has been proposed to have pro-atherogenic as well as anti-atherogenic properties. It hydrolyzes both phospholipids and TAG of lipoproteins, and its activity is negatively correlated with HDL levels. Although it is known that HL acts preferentially on HDL lipids, the basis for this specificity is not known, since it does not require any specific apoprotein for activity. In this study, we tested the hypothesis that sphingomyelin (SM), whose concentration is much higher in VLDL and LDL compared to HDL, is an inhibitor of HL, and that this could explain the lipoprotein specificity of the enzyme. The results presented show that the depletion of SM from normal lipoproteins activated the HL roughly in proportion to their SM content. SM depletion stimulated the hydrolysis of both phosphatidylcholine (PC) and TAG, although the PC hydrolysis was stimulated more. In the native lipoproteins, HL showed specificity for PC species containing polyunsaturated fatty acids at sn-2 position, and produced more unsaturated lyso PC species. The enzyme also showed preferential hydrolysis of certain TAG species over others. SM depletion affected the specificity of the enzyme towards PC and TAG species modestly. These results show that SM is a physiological inhibitor of HL activity in lipoproteins and that the specificity of the enzyme towards HDL is at least partly due to its low SM content.
authors.affiliation: Section of Endocrinology and Metabolism, Department of Medicine, University of Illinois at Chicago, 1819 West Polk M/C 797, Chicago, United States; Jesse Brown VA Medical Center, Chicago, IL, United States.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: Section of Endocrinology and Metabolism, Department of Medicine, University of Illinois at Chicago, 1819 West Polk M/C 797, Chicago, United States; Jesse Brown VA Medical Center, Chicago, IL, United States. Electronic address: psubbaia@uic.edu.
authors.forename: Papasani V
authors.initials: PV
authors.lastname: Subbaiah
mesh_terms: D006801:Humans; D008049:Lipase / Q000097:blood / Q000737:chemistry*; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000097:blood / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity / Q000502:physiology; D014280:Triglycerides / Q000097:blood / Q000737:chemistry
chemical_list: C504173:LIPC protein, human; D008074:Lipoproteins; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D014280:Triglycerides; D008049:Lipase
keywords: Enzyme inhibition; Hepatic lipase; PC species; Sphingomyelin; Substrate specificity; TAG species