title: Functional analysis of RNAi suppressor P19 on improving baculovirus yield and transgene expression in Sf9 cells.
abstract: OBJECTIVE
  To investigate whether RNA interference suppressor P19 derived from tombusvirus can enhance baculovirus yield and transgene expression.
  
  
  RESULTS
  A number of recombinant baculoviruses with P19 under the control of white spot syndrome virus immediate-early promoter, baculovirus early-to-late promoter, or P10 late promoter were constructed The budded virus titer and the expression levels of eGFP and luciferase were determined. P19 was clearly functional in Sf9 cells and could enhance baculovirus yield, eGFP and luciferase expression levels up to 6.8-, 1.8-, and 2.1-fold respectively, at 72 h post infection.
  
  
  CONCLUSIONS
  P19 enhanced baculovirus production and transgene expression, and thus has potential applications in baculovirus-based gene therapy and vaccine studies.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Yangkun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: China Institute of Veterinary Drug Control, Beijing, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China. duenqi227@126.com.
authors.forename: Enqi
authors.initials: E
authors.lastname: Du
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China. yzq8162@163.com.
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D061987:Sf9 Cells; D019076:Transgenes / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: Baculovirus; P19; RNAi suppressor; Sf9 cells; Transgene expression