title: Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery.
abstract: The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
authors.affiliation: †Institute of Biostructure and Bioimaging (IBB), CNR, via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Avitabile
authors.affiliation: ‡Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, 80134 Napoli, Italy.|§Interuniversity Research Centre on Bioactive Peptides (CIRPeB), via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Accardo
authors.affiliation: ‡Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Ringhieri
authors.affiliation: ‡Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, 80134 Napoli, Italy.|§Interuniversity Research Centre on Bioactive Peptides (CIRPeB), via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Morelli
authors.affiliation: §Interuniversity Research Centre on Bioactive Peptides (CIRPeB), via Mezzocannone 16, 80134 Napoli, Italy.|∥Institute of Crystallography (IC), CNR, Via Amendola 122, 70126 Bari, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Saviano
authors.affiliation: ⊥Department of Life Sciences and Biotechnologies, University of Ferrara, via Fossato di Mortara 74, 44121 Ferrara, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Montagner
authors.affiliation: ⊥Department of Life Sciences and Biotechnologies, University of Ferrara, via Fossato di Mortara 74, 44121 Ferrara, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Fabbri
authors.affiliation: ⊥Department of Life Sciences and Biotechnologies, University of Ferrara, via Fossato di Mortara 74, 44121 Ferrara, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Gallerani
authors.affiliation: ⊥Department of Life Sciences and Biotechnologies, University of Ferrara, via Fossato di Mortara 74, 44121 Ferrara, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Gambari
authors.affiliation: ‡Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, 80134 Napoli, Italy.|§Interuniversity Research Centre on Bioactive Peptides (CIRPeB), via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
mesh_terms: D015536:Down-Regulation; D016503:Drug Delivery Systems; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020014:K562 Cells; D008081:Liposomes; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D020135:Peptide Nucleic Acids / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: C519184:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000); D008081:Liposomes; C543800:MIRN210 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols