title: Evaluation of IGK and IGL molecular gene rearrangements according to the BIOMED-2 protocols for clinical diagnosis of Hodgkin lymphoma.
abstract: BACKGROUND
  Although the analysis of molecular clonality rearrangements of the immunoglobulin light chains (IGK and IGL) is an alternative approach for diagnosis of B cell non-Hodgkin lymphomas (NHLs) using BIOMED-2 protocols, NHLs have not been extensively confirmed for Hodgkin lymphoma (HL) cases. We evaluated BIOMED-2 protocols in HL cases, which have been suggested previously as gold standard method for molecular clonality analysis on formalin fixed, paraffin-embedded (FFPE) tissue in NHL patients.
  
  
  METHODS
  We recruited 50 consecutive FFPE tissues of HL samples to evaluate IGK and IGL clonality gene rearrangements using BIOMED-2 and Heteroduplex methods.
  
  
  RESULTS
  Our findings revealed a total of 94% (47/50) positive clonality, which consisted of 70% (35/50) for IGK and 44% (22/50) for IGL. In three cases, clonality was not detected in any of the immunoglobulin gene segments.
  
  
  CONCLUSIONS
  Analysis of clonality gene rearrangements in IGK and IGL genes using BIOMED-2 protocols could be implemented as a valuable method for improving clonality detection rate in HL cases and sensitivity (94%) and accuracy of HL diagnosis similar to that of the NHL samples will be increased.
authors.affiliation: a Department of Biology, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghorbian
authors.affiliation: b Department of Pathology, Imam Khomeini Hospital Complex , Medical Sciences/University of Tehran , Iran.
authors.forename: Issa
authors.initials: I
authors.lastname: Jahanzad
authors.affiliation: a Department of Biology, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Javadi
authors.affiliation: c Biotechnology Research Center , Tabriz University of Medical Sciences , Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Sakhinia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D015321:Gene Rearrangement; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: C014609:IgK; D007136:Immunoglobulins
keywords: BIOMED-2; Clonality; Hodgkin's lymphoma; IGK; Rearrangements