title: Rational, yet simple, design and synthesis of an antifreeze-protein inspired polymer for cellular cryopreservation.
abstract: Antifreeze (glyco) proteins AF(G)Ps are potent ice recrystallization inhibitors, which is a desirable property to enhance cryopreservation of donor tissue/cells. Here we present the rational synthesis of a new, biomimetic, ice-recrystallization inhibiting polymer derived from a cheap commodity polymer, based on an ampholyte structure. The polymer is used to enhance the cryopreservation of red blood cells, demonstrating a macromolecular solution to tissue storage.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. m.i.gibson@warwick.ac.uk.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Mitchell
authors.forename: Neil R
authors.initials: NR
authors.lastname: Cameron
authors.forename: Matthew I
authors.initials: MI
authors.lastname: Gibson
mesh_terms: D000818:Animals; D021301:Antifreeze Proteins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007053:Ice / Q000032:analysis*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D021301:Antifreeze Proteins; D003451:Cryoprotective Agents; D007053:Ice; D011108:Polymers