title: Strain and sex differences in puberty onset and the effects of THC administration on weight gain and brain volumes.
abstract: The use of recreational marijuana is widespread and frequently begins and persists through adolescence. Some research has shown negative consequences of adolescent marijuana use, but this is not seen across studies, and certain factors, like genetic background and sex, may influence the results. It is critical to identify which characteristics predispose an individual to be susceptible to the negative consequences of chronic exposure to marijuana in adolescence on brain health and behavior. To this end, using males and females of two strains of rats, Long-Evans hooded (LER) and Wistar (WR) rats, we explored whether these anatomically and behaviorally dimorphic strains demonstrated differences in puberty onset and strain-specific effects of adolescent exposure to Δ9-tetrahydrocannabinol (THC), the main psychoactive component of marijuana. Daily 5 mg/kg treatment began on the day of puberty onset and continued for 14 days. Of particular interest were metrics of growth and volumetric estimates of brain areas involved in cognition that contain high densities of cannabinoid receptors, including the hippocampus and its subregions, the amygdala, and the frontal cortex. Brain volumetrics were analyzed immediately following the treatment period. LER and WR females started puberty at different ages, but no strain differences were observed in brain volumes. THC decreased weight gain throughout the treatment period for all groups. Only the hippocampus and some of its subregions were affected by THC, and increased volumes with THC administration was observed exclusively in females, regardless of strain. Long-term treatment of THC did not affect all individuals equally, and females displayed evidence of increased sensitivity to the effects of THC, and by extension, marijuana. Identifying differences in adolescent physiology of WR and LER rats could help determine the cause for strain and sex differences in brain and behavior of adults and help to refine the use of animal models in marijuana research.
authors.affiliation: University of Lethbridge, 4401 University Drive, Lethbridge, AB T1K 3M4, Canada. Electronic address: rj.keeley@uleth.ca.
authors.forename: R J
authors.initials: RJ
authors.lastname: Keeley
authors.affiliation: University of Lethbridge, 4401 University Drive, Lethbridge, AB T1K 3M4, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Trow
authors.affiliation: University of Lethbridge, 4401 University Drive, Lethbridge, AB T1K 3M4, Canada.
authors.forename: R J
authors.initials: RJ
authors.lastname: McDonald
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects*; D013759:Dronabinol / Q000494:pharmacology*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D008297:Male; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D020318:Rats, Long-Evans; D017208:Rats, Wistar; D012727:Sex Characteristics; D012741:Sexual Maturation / Q000187:drug effects*; D013045:Species Specificity; D013997:Time Factors; D014621:Vagina / Q000166:cytology / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D011619:Psychotropic Drugs; D013759:Dronabinol
keywords: THC; adolescence; hippocampus; rat; sex difference; strain difference