title: Tunable Thermoresponsiveness of Resilin via Coassembly with Rigid Biopolymers.
abstract: The ability to tune the thermoresponsiveness of recombinant resilin protein, Rec1-resilin, through a facile coassembly system was investigated in this study. The effects of change in conformation and morphology with time and the responsive behavior of Rec1-resilin in solution were studied in response to the addition of a rigid model polypeptide (poly-l-proline) or a hydrophobic rigid protein (Bombyx mori silk fibroin). It was observed that by inducing more ordered conformations and increasing the hydrophobicity the lower critical solution temperature (LCST) of the system was tuned to lower values. Time and temperature were found to be critical parameters in controlling the coassembly behavior of Rec1-resilin in both the model polypeptide and more complex protein systems. Such unique properties are useful for a wide range of applications, including drug delivery and soft tissue engineering applications.
authors.affiliation: †Ian Wark Research Institute, University of South Australia, Mawson Lakes Boulevard, Adelaide, South Australia 5095, Australia.
authors.forename: Jasmin L
authors.initials: JL
authors.lastname: Whittaker
authors.affiliation: †Ian Wark Research Institute, University of South Australia, Mawson Lakes Boulevard, Adelaide, South Australia 5095, Australia.
authors.forename: Naba K
authors.initials: NK
authors.lastname: Dutta
authors.affiliation: ‡Bragg Institute, ANSTO, Lucas Heights, New South Wales 2234, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Knott
authors.affiliation: §Mawson Institute, University of South Australia, Mawson Lakes Boulevard, Adelaide, South Australia 5095, Australia.
authors.forename: Gordon
authors.initials: G
authors.lastname: McPhee
authors.affiliation: §Mawson Institute, University of South Australia, Mawson Lakes Boulevard, Adelaide, South Australia 5095, Australia.
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
authors.affiliation: ∥CSIRO Agriculture, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Queensland 4067, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Elvin
authors.affiliation: ⊥CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria 3168, Australia.
authors.forename: Anita
authors.initials: A
authors.lastname: Hill
authors.affiliation: †Ian Wark Research Institute, University of South Australia, Mawson Lakes Boulevard, Adelaide, South Australia 5095, Australia.
authors.forename: Namita Roy
authors.initials: NR
authors.lastname: Choudhury
mesh_terms: D000818:Animals; D012831:Bombyx; D005349:Fibroins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D019476:Insect Proteins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013696:Temperature
chemical_list: D019476:Insect Proteins; D010455:Peptides; C011083:polyproline; C030951:resilin; D005349:Fibroins