title: Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis.
abstract: Understanding the molecular components of immune recognition of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, can help designing novel strategies to combat TB. Here, we identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacterial mannose-capped lipoarabinomannan as a primary ligand for CL-LK. Mice deficient in CL-K1, one of the CL-LK subunits, do not display altered susceptibility to M. tuberculosis. However, we found that the amount of CL-LK in the serum of patients with active TB is reduced, compared to that in controls, and correlates inversely to the magnitude of the immune response to the pathogen. These findings indicate that CL-LK might be of interest for future diagnostic and treatment monitoring purposes.
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Troegeler
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Geanncarlo
authors.initials: G
authors.lastname: Lugo-Villarino
authors.affiliation: Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Hansen
authors.affiliation: Mycobacteria Unit, Pasteur Institute in Antananarivo, Antananarivo, Madagascar.
authors.forename: Voahangy
authors.initials: V
authors.lastname: Rasolofo
authors.affiliation: Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
authors.forename: Maiken Lumby
authors.initials: ML
authors.lastname: Henriksen
authors.affiliation: Department of Microbiology & Immunochemistry, Asahikawa Medical University, Asahikawa, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Mori
authors.affiliation: Department of Microbiology & Immunochemistry, Asahikawa Medical University, Asahikawa, Japan.
authors.forename: Katsuki
authors.initials: K
authors.lastname: Ohtani
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Duval
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Mercier
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Alan
authors.initials: A
authors.lastname: Bénard
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Jérome
authors.initials: J
authors.lastname: Nigou
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Hudrisier
authors.affiliation: Department of Microbiology & Immunochemistry, Asahikawa Medical University, Asahikawa, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Wakamiya
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Neyrolles
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D037222:Collectins / Q000097:blood / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000472:pathogenicity; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000276:immunology
chemical_list: C518521:Colec11 protein, human; D037222:Collectins; D008024:Ligands; D008070:Lipopolysaccharides; D051192:Receptors, Pattern Recognition; C050016:lipoarabinomannan