title: Differential Expression of Extracellular Lipase and Protease Activities of Mycelial and Yeast Forms in Malassezia furfur.
abstract: Malassezia furfur is a dimorphic yeast that is part of the human skin microflora. This fungus is a pathogen of a certain skin diseases, such as pityriasis versicolor, and in rare cases causes systemic infection in neonates. However, the role of dimorphism in the pathogenicity remains unclear. A modified induction medium (IM) was successfully able to induce mycelial growth of M. furfur under both solid and liquid condition. Filamentous elements with branching hyphae were observed when cultured in the IM. Furthermore, addition of bovine fetus serum into the liquid IM did not promote hyphal formation; on the contrary, it retrograded hyphae to the yeast form. Plate-washing assay showed that M. furfur hyphae did not possess the ability of invasive growth. Secretory proteins from both yeast and hyphal forms were isolated, and lipase and protease activities were analyzed. Intriguingly, the hyphal form showed higher activities than those of the yeast form, particularly the protease activity.
authors.affiliation: Department of Biology, Faculty of Science and Technology, Chiang Mai Rajabhat University, 202 Chang Phuak Road, Muang, Chiang Mai, 50300, Thailand, weerapong.juntachai@gmail.com.
authors.forename: Weerapong
authors.initials: W
authors.lastname: Juntachai
authors.forename: Susumu
authors.initials: S
authors.lastname: Kajiwara
mesh_terms: D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D008049:Lipase / Q000032:analysis*; D008290:Malassezia / Q000166:cytology* / Q000201:enzymology* / Q000254:growth & development; D025282:Mycelium / Q000201:enzymology* / Q000254:growth & development; D010447:Peptide Hydrolases / Q000032:analysis*
chemical_list: D003470:Culture Media; D008049:Lipase; D010447:Peptide Hydrolases