title: Immunosuppressive effects of mesenchymal stem cell transplantation in rat burn models.
abstract: OBJECTIVE
  This study is to investigate the effects of mesenchymal stem cell (MSC) transplantation in burn treatment.
  
  
  METHODS
  Wharton's Jelly was stripped from neonatal umbilical cord, and human umbilical cord MSCs were then cultured. Burn models were constructed in male SD rats weighted at 200±5 g, and the rats were randomly divided into control and MSCs transplantation groups. The rats in transplantation group were injected subcutaneously with MSCs (2×10(6)) at 24 h after burning. Blood samples were collected at 0 d, 1 d, 2 d, 3 d, 5 d and 7 d after burning and the contents of white blood cells (WBC), C-reactive protein (CRP), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10 ) were detected. The wound healing rate at 7 d, 14 d, 21 d and 28 d together with the wound healing time were compared and analyzed statistically by analysis of variance (ANOVA).
  
  
  RESULTS
  WBC and CRP in control group increased significantly at 1 d and 2 d, 2 d and 3 d, respectively. IFN-γ, IL-6 and IL-10 levels in serum showed increasing till 5th day and TNF-α arrived its peak value at 7th day. By contrast, WBC, CRP, TNF-α, IL-6 and IL-10 in the MSCs transplantation group showed slight increase after burning and the differences were verified by statistically analysis. IFN-γ showed no significant difference between the two groups. MSCs transplantation group showed significantly higher wound healing rate at 14 d, 21 d, 28 d and showed shorter wound healing time than control.
  
  
  CONCLUSIONS
  MSCs transplantation could suppress secondary inflammatory reaction by lowering inflammatory cytokines after burning, thus promoting wound healing and scald repair in burn animal model.
authors.affiliation: Department of Blood Transfusion, General Hospital of Urumqi Urumqi 830000, P. R. China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Xinjiang Laboratory of Hydatid fundamental Medicine, First Affiliated Hospital of Xinjiang Medical University Urumqi 830011, Xinjiang, P. R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: La
authors.affiliation: Department of Blood Transfusion, General Hospital of Urumqi Urumqi 830000, P. R. China.
authors.forename: Lixing
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Ophthalmology, The 474th Hospital of People's Liberation Army Urumqi 830000, P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Blood Transfusion, General Hospital of Urumqi Urumqi 830000, P. R. China.
authors.forename: Yuanlun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Blood Transfusion, General Hospital of Urumqi Urumqi 830000, P. R. China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Xinjiang Laboratory of Hydatid fundamental Medicine, First Affiliated Hospital of Xinjiang Medical University Urumqi 830011, Xinjiang, P. R. China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Blood Transfusion, General Hospital of Urumqi Urumqi 830000, P. R. China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Xing
mesh_terms: D000818:Animals; D002056:Burns / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
keywords: MSCs; burn; cytokines; immunoregulation; immunosuppression; transplantation