title: Isolation and characterization of a bacteriophage with broad host range, displaying potential in preventing bovine diarrhoea.
abstract: Phage therapy has been previously tried for treatment of diarrhoea in calves, pigs and lambs but those trials were conducted without any detailed information of used phages. Here, we report isolation of a broad-spectrum phage which showed bactericidal activity against 47.3 % of calf diarrhoeal isolates of Escherichia coli, in vitro. The isolated phage resembled the characteristics of Myoviridae family and showed ~97 % similarity with earlier reported bacteriophages of sub family-Tevenvirinae, genus-T4-like virus, based on nucleotide sequence of major head protein-gp23 gene. The phage exhibits the potential to be used as drug substitute tool against E. coli causing diarrhoea in cattle in farm environments.
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India. tarunandri@gmail.com.
authors.forename: Taruna
authors.initials: T
authors.lastname: Anand
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India.
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Vaid
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India.
authors.forename: Bidhan Ch
authors.initials: BCh
authors.lastname: Bera
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Barua
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India.
authors.forename: T
authors.initials: T
authors.lastname: Riyesh
authors.affiliation: National Research Centre on Equines, Sirsa Road, Hisar, India.
authors.forename: N
authors.initials: N
authors.lastname: Virmani
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Yadav
authors.affiliation: Veterinary Type Culture Collection, Sirsa Road, Hisar, Haryana, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Malik
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification* / Q000502:physiology* / Q000648:ultrastructure; D001691:Biological Therapy / Q000379:methods; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000517:prevention & control / Q000662:veterinary; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary; D058507:Host Specificity; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D017361:Viral Nonstructural Proteins
keywords: Bacteriophage; Diarrhoea; E. coli; Electron microscopy; Myoviridae