title: Rapid Screening of Gli2/3 Mutants Using the Flp-In System.
abstract: Gli2 and Gli3 respond to the Hedgehog (Hh) signal in mammals by undergoing posttranslational modifications and moving to the nucleus. The study of Gli proteins has been hampered by the fact that their overexpression in cells prevents their proper regulation. To address this issue, we have developed a method of rapid generation of stable cell lines expressing near-endogenous and approximately equal levels of wild-type and mutant Gli proteins. This method is applicable to the study of effects of various mutations on Gli protein modifications and activity.
authors.affiliation: Department of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093, Warszawa, Poland, p.niewiadomski@nencki.gov.pl.
authors.forename: Pawel
authors.initials: P
authors.lastname: Niewiadomski
authors.forename: Rajat
authors.initials: R
authors.lastname: Rohatgi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015870:Gene Expression; D059765:Homologous Recombination; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D051379:Mice; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D014162:Transfection; D000075746:Zinc Finger Protein Gli2; D000074290:Zinc Finger Protein Gli3
chemical_list: C000618551:Gli2 protein, mouse; C465571:Gli3 protein, mouse; D051741:Kruppel-Like Transcription Factors; D050505:Mutant Proteins; D009419:Nerve Tissue Proteins; D000075746:Zinc Finger Protein Gli2; D000074290:Zinc Finger Protein Gli3