title: Characterization of a novel y-type HMW-GS with eight cysteine residues from Triticum monococcum ssp. monococcum.
abstract: The composition and number of high-molecular-weight glutenin subunits (HMW-GSs) play important roles in determining the grain-processing quality of common wheat. The Glu-1Ay allele is silent in common wheat. In this study, an active y-type HMW-GS allele termed 1Ay8.2 (GenBank No. KP137569) was identified from Triticum monococcum L. ssp. monococcum (AmAm, 2n=2x=14), a species with a genome related to the A-genome of common wheat. Compared with previously reported active 1Ay subunits, this novel subunit contained an extra cysteine residue at position 103 of the amino acid sequence in the N-terminal region, in addition to the six cysteines in the N- and C-terminal regions found in most active 1Ay subunits and the one in the repetitive region that appears in only a few 1Ay alleles. This subunit was expressed in an amphiploid (AAAmAmBB, 2n=6x=42) between Triticum turgidum L. ssp. dicoccon and T. monococcum ssp. monococcum. This amphiploid could be used as a bridge to transfer 1Ay8.2 into common wheat cultivars. Replacing the silenced 1Ay in common wheat with the active 1Ay8.2 allele harboring an extra cysteine residue is expected to improve the quality by increasing the number of HMW-GSs and promoting the formation of covalent interactions through disulfide bonds with the extra cysteine residue.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Zenglin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Kou
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Shunzong
authors.initials: S
authors.lastname: Ning
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: School of Agriculture and Technology, Zunyi Normal College, Guizhou 563002, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Youliang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Dengcai
authors.initials: D
authors.lastname: Liu
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China. Electronic address: zhanglianquan1977@126.com.
authors.forename: Lianquan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D003545:Cysteine / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D014908:Triticum / Q000737:chemistry* / Q000145:classification
chemical_list: D005983:Glutens; C037302:glutenin; D003545:Cysteine
keywords: 1Ay; Amphiploid; Cysteine; HMW glutenin subunits; Triticum monococcum