title: Evolution of cytokine profile during the treatment of cerebral toxoplasmosis in HIV-infected patients.
abstract: This study was to follow IFN-γ, TNF-α and IL-10 modulation of peripheral blood mononuclear cells (PBMC) from HIV/cerebral toxoplasmosis patients (CT) during specific treatment. The results were compared with two other groups: HIV patients that had CT at least one year before (P/CT) and individuals with chronic toxoplasmosis (CHR). Blood samples (63) collected from three groups were analyzed. CT, 15 patients (3 blood samples collected one day before Toxoplasma gondii treatment; 7 and 15days during the treatment). P/CT, 5 patients (one blood sample collected at least, one year after the treatment). CHR, 13 individuals with chronic toxoplasmosis (one blood sample). Cytokine levels were assessed by ELISA after PBMC stimulation with T. gondii antigen. CT patients had low IFN-γ; discrete increase at 7th and 15th days; and the levels were recovered in cured patients (P/CT). CT patients had high TNF-α in the beginning of the treatment. TNF-α levels decrease during the treatment (7th and 15th) and in those patients who were treated (P/CT). IL-10 levels were almost similar in CT and P/CT groups but low when compared with CHR individuals. The evolution of the infection was correlated to restoration of IFN-γ response and a decrease of the inflammation. The evaluation of the immune response can provide valuable information and better monitoring of patients during specific treatment.
authors.affiliation: Centro de Parasitologia e Micologia do Instituto Adolfo Lutz, SP, Brazil.
authors.forename: Cristina da Silva
authors.initials: Cda S
authors.lastname: Meira
authors.affiliation: Centro de Parasitologia e Micologia do Instituto Adolfo Lutz, SP, Brazil. Electronic address: pchioccola@gmail.com.
authors.forename: Vera Lucia
authors.initials: VL
authors.lastname: Pereira-Chioccola
authors.affiliation: Departamento de Neurologia, Instituto de Infectologia Emílio Ribas, SP, Brazil.
authors.forename: José Ernesto
authors.initials: JE
authors.lastname: Vidal
authors.affiliation: Centro de Parasitologia e Micologia do Instituto Adolfo Lutz, SP, Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Motoie
authors.affiliation: Centro de Parasitologia e Micologia do Instituto Adolfo Lutz, SP, Brazil.
authors.forename: Thaís Alves da
authors.initials: TA
authors.lastname: Costa-Silva
authors.affiliation: Centro de Parasitologia e Micologia do Instituto Adolfo Lutz, SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gava
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D000953:Antigens, Protozoan / Q000276:immunology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014122:Toxoplasma / Q000187:drug effects / Q000276:immunology; D016781:Toxoplasmosis, Cerebral / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; C508609:IL10 protein, human; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Acquired immunodeficiency syndrome; Central nervous system; Cerebral toxoplasmosis; Cytokines; Immune system; Toxoplasma gondii; Toxoplasmosis