title: Coordinate expression of cytokeratins 7 and 14, vimentin, and Bcl-2 in canine cutaneous epithelial tumors and cysts.
abstract: Forty-seven canine cutaneous epithelial tumors and cysts were examined to determine coordinate expression of cytokeratins 7 (CK7) and 14 (CK14), vimentin, and Bcl-2 using commercially available antibodies. Within non-affected normal skin adjacent to tumors or cysts, CK7 expression was observed in luminal cells in apocrine glands; CK14 expression was observed in the stratum basale, stratum spinosum, stratum granulosum, basal layer of outer root sheath, sebaceous glands, and myoepithelial cells of apocrine glands; vimentin expression was observed in dermal papilla and scattered non-epithelial cells within the epidermis; and Bcl-2 expression was observed in scattered non-epithelial cells in the epidermis and some apocrine glands. The pattern of expression of CK7 and CK14 in cases of adenocarcinoma of the apocrine gland of the anal sac (CK7+/CK14-) and hepatoid gland tumors (CK7-/CK14+) may prove useful for diagnostic purposes. Loss of expression of CK14 and vimentin, identifying myoepithelial cells, was observed in apocrine and ceruminous adenocarcinomas. Differences in patterns of expression of Bcl-2 were observed between infundibular keratinizing acanthomas compared to trichoepitheliomas.
authors.affiliation: Department of Veterinary Clinical Medicine (Pieper, Campbell), University of Illinois, Urbana, ILDepartment of Pathobiology (Stern), University of Illinois, Urbana, ILVeterinary Diagnostic Laboratory (LeClerc), University of Illinois, Urbana, IL jpieper@illinois.edu.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Pieper
authors.affiliation: Department of Veterinary Clinical Medicine (Pieper, Campbell), University of Illinois, Urbana, ILDepartment of Pathobiology (Stern), University of Illinois, Urbana, ILVeterinary Diagnostic Laboratory (LeClerc), University of Illinois, Urbana, IL.
authors.forename: Adam W
authors.initials: AW
authors.lastname: Stern
authors.affiliation: Department of Veterinary Clinical Medicine (Pieper, Campbell), University of Illinois, Urbana, ILDepartment of Pathobiology (Stern), University of Illinois, Urbana, ILVeterinary Diagnostic Laboratory (LeClerc), University of Illinois, Urbana, IL.
authors.forename: Suzette M
authors.initials: SM
authors.lastname: LeClerc
authors.affiliation: Department of Veterinary Clinical Medicine (Pieper, Campbell), University of Illinois, Urbana, ILDepartment of Pathobiology (Stern), University of Illinois, Urbana, ILVeterinary Diagnostic Laboratory (LeClerc), University of Illinois, Urbana, IL.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Campbell
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary; D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D001050:Apocrine Glands / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism*; D003560:Cysts / Q000378:metabolism / Q000662:veterinary; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism*; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D053547:Keratin-14 / Q000276:immunology / Q000378:metabolism; D053552:Keratin-7 / Q000276:immunology / Q000378:metabolism; D011237:Predictive Value of Tests; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000276:immunology / Q000378:metabolism; D012627:Sebaceous Glands / Q000378:metabolism; D012878:Skin Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*; D013544:Sweat Gland Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary; D014746:Vimentin / Q000276:immunology / Q000378:metabolism
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D053547:Keratin-14; D053552:Keratin-7; D019253:Proto-Oncogene Proteins c-bcl-2; D014746:Vimentin
keywords: Bcl-2; canine; cytokeratin 14; cytokeratin 7; immunohistochemistry; skin; tumor; vimentin