title: Reduction in mdx mouse muscle degeneration by low-intensity endurance exercise: a proteomic analysis in quadriceps muscle of exercised compared with sedentary mdx mice.
abstract: In our recent study was shown a significant recovery of damaged skeletal muscle of mice with X-linked muscular dystrophy (mdx) following low-intensity endurance exercise, probably by reducing the degeneration of dystrophic muscle. Consequently, in the present work, we aimed to identify proteins involved in the observed reduction in degenerating fibres. To this end, we used proteomic analysis to evaluate changes in the protein profile of quadriceps dystrophic muscles of exercised compared with sedentary mdx mice. Four protein spots were found to be significantly changed and were identified as three isoforms of carbonic anhydrase 3 (CA3) and superoxide dismutase [Cu-Zn] (SODC). Protein levels of CA3 isoforms were significantly up-regulated in quadriceps of sedentary mdx mice and were completely restored to wild-type (WT) mice values, both sedentary and exercised, in quadriceps of exercised mdx mice. Protein levels of SODC were down-regulated in quadriceps of sedentary mdx mice and were significantly restored to WT mice values, both sedentary and exercised, in quadriceps of exercised mdx mice. Western blot data were in agreement with those obtained using proteomic analysis and revealed the presence of one more CA3 isoform that was significantly changed. Based on data found in the present study, it seems that low-intensity endurance exercise may in part contribute to reduce cell degeneration process in mdx muscles, by counteracting oxidative stress.
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, I-90127 Palermo, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Fontana
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, I-90127 Palermo, Italy.
authors.forename: Odessa
authors.initials: O
authors.lastname: Schillaci
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Physiology, University of Palermo, I-90134 Palermo, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Frinchi
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, I-90127 Palermo, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Giallombardo
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Physiology, University of Palermo, I-90134 Palermo, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Morici
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Physiology, University of Palermo, I-90134 Palermo, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Di Liberto
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, I-90127 Palermo, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Alessandro
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Physiology, University of Palermo, I-90134 Palermo, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: De Leo
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, I-95125 Catania, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Perciavalle
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Physiology, University of Palermo, I-90134 Palermo, Italy.
authors.forename: Natale
authors.initials: N
authors.lastname: Belluardo
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, Section of Physiology, University of Palermo, I-90134 Palermo, Italy giuseppa.mudo@unipa.it.
authors.forename: Giuseppa
authors.initials: G
authors.lastname: Mudò
mesh_terms: D000818:Animals; D015153:Blotting, Western; D024403:Carbonic Anhydrase III / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008297:Male; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009124:Muscle Proteins / Q000378:metabolism*; D020388:Muscular Dystrophy, Duchenne / Q000503:physiopathology; D010807:Physical Endurance / Q000502:physiology*; D040901:Proteomics / Q000379:methods; D052097:Quadriceps Muscle / Q000378:metabolism* / Q000503:physiopathology*; D015203:Reproducibility of Results; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D013482:Superoxide Dismutase; D024403:Carbonic Anhydrase III
keywords: X-linked muscular dystrophy (mdx); carbonic anhydrase; exercise; muscle oxidative stress; muscle proteomic; muscular dystrophy