title: Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.
abstract: Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK.
authors.forename: David
authors.initials: D
authors.lastname: Bitto
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: Steinar
authors.initials: S
authors.lastname: Halldorsson
authors.forename: Katie J
authors.initials: KJ
authors.lastname: Doores
authors.forename: Laura K
authors.initials: LK
authors.lastname: Pritchard
authors.forename: Juha T
authors.initials: JT
authors.lastname: Huiskonen
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Bowden
authors.forename: Max
authors.initials: M
authors.lastname: Crispin
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D007477:Ions / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D007477:Ions; D011134:Polysaccharides; D014764:Viral Proteins