title: A rapid spin through oil results in higher cell-associated concentrations of antiretrovirals compared with conventional cell washing.
abstract: BACKGROUND
  Determination of cell-associated antiretroviral drug concentrations is necessary for research into reservoirs of HIV. Variation exists in cell-associated drug concentrations among research groups. One cause for this may be washing cells during processing. We explored spinning cells through oil to minimize this variability.
  
  
  RESULTS
  Raltegravir, atazanavir, darunavir, efavirenz, lopinavir and ritonavir concentrations were assessed in CEM.ss T cells washed with HBSS and oil-spun cells. Oil-spun cells had significantly higher concentrations for all drugs compared with samples washed with HBSS.
  
  
  CONCLUSIONS
  The decline in cell-associated drug concentrations with saline washes compared with a single spin through oil shows the utility of a spin through oil. Oil centrifugation results in high cell-associated drug concentrations, and can be done in a fast, efficient manner.
authors.affiliation: Antiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Theodore J
authors.initials: TJ
authors.lastname: Cory
authors.affiliation: Antiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Lee C
authors.initials: LC
authors.lastname: Winchester
authors.affiliation: Antiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Brian L
authors.initials: BL
authors.lastname: Robbins
authors.affiliation: Antiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Courtney V
authors.initials: CV
authors.lastname: Fletcher
mesh_terms: D044966:Anti-Retroviral Agents / Q000032:analysis / Q000737:chemistry*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D017320:HIV Protease Inhibitors / Q000032:analysis / Q000737:chemistry; D006801:Humans; D007552:Isotonic Solutions / Q000737:chemistry; D007553:Isotope Labeling; D013058:Mass Spectrometry; D009821:Oils / Q000737:chemistry*
chemical_list: D044966:Anti-Retroviral Agents; D017320:HIV Protease Inhibitors; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D009821:Oils