title: Reduced expression of CRHR2 and Sp-1 in myocardium of ovariectomized rats is improved by exercise training.
abstract: Exercise training has been looked on as a non-pharmacologic approach to treating ovariectomy (OVX)-induced dysfunctions. In this study, we investigated whether chronic exercise impacts on expression of urocortins (UCNs) and corticotropin-releasing hormone receptor type 2 (CRHR2) in myocardium of OVX rats. Bilateral OVX or sham-operation was performed under anesthesia. Both groups were then divided into two subgroups, with or without treadmill training for 8 weeks. It was found that OVX as well as exercise did not affect the mRNA levels of UCN, UCN2 and UCN3 in myocardium. OVX caused down-regulation of CRHR2 in myocardium. Exercise training reversed the OVX-induced reduction of CRHR2, but had no influence on CRHR2 level in sham rats. OVX resulted in a decrease in estrogen receptor α (ERα) expression in myocardium, which was restored by exercise. Moreover, exercise training also reversed OVX-induced down-regulation of specific protein-1 (Sp-1) expression in myocardium. CRHR2 expression level correlated with Sp-1 and ERα level in myocardium. These results indicate that exercise training can restore the CRHR2 level in myocardium of OVX rats, which is associated with ERα and Sp-1 expression.
authors.affiliation: a School of Kinesiology, Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport , Shanghai , China and.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Tang
authors.affiliation: a School of Kinesiology, Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport , Shanghai , China and.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: b Department of Physiology , Second Military Medical University , Shanghai , China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: b Department of Physiology , Second Military Medical University , Shanghai , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ni
authors.affiliation: b Department of Physiology , Second Military Medical University , Shanghai , China.
authors.forename: Binhai
authors.initials: B
authors.lastname: Cong
authors.affiliation: a School of Kinesiology, Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport , Shanghai , China and.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D009206:Myocardium / Q000378:metabolism*; D010052:Ovariectomy; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D054832:Urocortins / Q000235:genetics* / Q000378:metabolism
chemical_list: C101531:CRF receptor type 2; D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D016329:Sp1 Transcription Factor; C000607145:Ucn protein, rat; D054832:Urocortins; C514216:urocortin 3, rat; D003346:Corticotropin-Releasing Hormone
keywords: Corticotropin-releasing hormone receptor type 2; estrogens; exercise training; myocardium; urocortin