title: Enhanced vasorelaxation effect of endogenous anandamide on thoracic aorta in renal vascular hypertension rats.
abstract: Emerging evidence has indicated that anandamide (AEA) is able to stimulate vasorelaxation in both spontaneously hypertensive rats (SHRs) and L-NAME-induced hypertensive rats. Yet it remains unknown whether AEA modulates vasomotion of the aorta in renovascular hypertensive (RVH) rats. The aim of present study is to explore the effect of AEA on the relaxation of thoracic aortas in two-kidney one-clip (2K1C)-induced RVH rats. It is demonstrated that AEA stimulates a pronounced relaxation in the aortas of 2K1C rats compared with sham rats. The enhanced relaxation caused by AEA in aortas from 2K1C rats was diminished in the presence of the cannabinoid receptor-1 (CB1) antagonist AM251 and the CB2receptor antagonist AM630. Likewise, the vasodilation action of AEA was blocked in L-NAME-treated or endothelium-denuded aortas. The Western blot results revealed that the expression of CB1and CB2receptors was increased in the 2K1C rat aortas compared with sham rats. The phosphorylation of endothelial nitric oxide synthase (p-eNOS) at the activation site Ser1177 was enhanced in AEA-treated rings from 2K1C rats in both time-dependent and dose-dependent manners. The augmented p-eNOS expression was inhibited by the co-treatment with AM251 or AM630. Taken together, the present study demonstrated that AEA enhanced endothelium-dependent aortic relaxation through activation of both CB1and CB2receptors and P-eNOS/NO pathway in 2K1C rats.
authors.affiliation: Department of Physiology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Physiology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Yi-Xian
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Department of Physiology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Physiology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Ma
authors.affiliation: Institute for Cardiology of Siberian Branch under the Russian Academy of Medical Sciences, Tomsk, Russia.
authors.forename: Leonid
authors.initials: L
authors.lastname: Maslov
authors.affiliation: Department of Physiology, Hebei Medical University, Shijiazhuang, China.|Hebei Collaborative Innovation Centre for Cardio-cerebrovascular Disease, Shijiazhuang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
keywords: 
  eNOS
  ; anandamide; cannabinoid receptor-1; cannabinoid receptor-2; renal vascular hypertension