title: [Effect of Hypericin on the Function of the Neuroretina: An Electroretinographic Study].
abstract: BACKGROUND
  Hypericin is an important component of the Saint John's wort (Hypericum perforatum). It is assumed to inhibit intracellular signalling cascades, which contribute to neoangiogenesis. The phototoxic effect of hypericin on the retina was investigated in human retinal pigment epithelium (RPE); hypericin induces oxidative stress and has also been described to be an inhibitor of Ca(2+) influx channel in cultured RPE cells. The aim of our study is to evaluate the effect of hypericine on the function of the neuroretina.
  
  
  METHODS
  Isolated bovine retinas were perfused with an oxygen saturated nutrient solution (1 mL/min). We exposed the retina to a flashlight of 6.3 mlx every 5 min. The electroretinogram (ERG) was recorded as a transretinal potential using Ag/AgCl electrodes. ERGs were monitored before, during and after hypericin exposure.
  
  
  RESULTS
  In three independent experiments we investigated the effect of hypericin on the amplitude of the b-wave. In our experiments we observed a significant reduction of the amplitude of the b-wave to 87.1 ± 3.5 % (p = 0.02). This reduction was in all our experiments partially reversible. After hypericin wash-out the b-wave amplitude did not recover completely and did not return to the initial value (91.0 ± 5.1 %; not significant). We did not observe a significant effect of hypericin on the implicit time of the b-wave.
  
  
  CONCLUSIONS
  This study shows for the first time that hypericin influences retinal signal transduction, suggesting that hypericin impairs not only the RPE, but also affects retinal signalling and function.
authors.affiliation: Institut für Neurophysiologie, Universität zu Köln.
authors.forename: M
authors.initials: M
authors.lastname: Alnawaiseh
authors.affiliation: Institut für Neurophysiologie, Universität zu Köln.
authors.forename: W
authors.initials: W
authors.lastname: Albanna
authors.affiliation: Institut für Neurophysiologie, Universität zu Köln.
authors.forename: R
authors.initials: R
authors.lastname: Abumuaileq
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum Münster.
authors.forename: M R R
authors.initials: MR
authors.lastname: Böhm
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum Münster.
authors.forename: N
authors.initials: N
authors.lastname: Eter
authors.affiliation: Institut für Neurophysiologie, Universität zu Köln.
authors.forename: T
authors.initials: T
authors.lastname: Schneider
mesh_terms: D000818:Animals; D000873:Anthracenes; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004596:Electroretinography / Q000187:drug effects* / Q000379:methods*; D066298:In Vitro Techniques; D008027:Light; D010569:Perylene / Q000008:administration & dosage / Q000031:analogs & derivatives*; D010775:Photic Stimulation / Q000379:methods*; D020419:Photoreceptor Cells, Vertebrate / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D055213:Retinal Pigment Epithelium / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects
chemical_list: D000873:Anthracenes; D010569:Perylene; C004965:hypericin