title: Effects of cellular viscoelasticity in lifetime extraction of single receptor-ligand bonds.
abstract: Single-molecule force spectroscopy is widely used to determine kinetic parameters of dissociation by analyzing bond rupture data obtained via applying mechanical force to cells, capsules, and beads that are attached to an intermolecular bond. The bond rupture data are obtained in experiments either at a constant force or at a constant loading rate. We explore the effect of cellular viscoelasticity in constant-force experiments. Specifically, we perform Monte Carlo simulations of bond rupture at a given constant force to obtain the bond lifetime as a function of force in the absence and in the presence of bond force modulation due to cellular viscoelasticity, to explore its effect on the bond lifetime.
authors.affiliation: Colorado State University, Fort Collins, Colorado 80523, USA.
authors.forename: V K
authors.initials: VK
authors.lastname: Gupta
mesh_terms: D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D004548:Elasticity; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D009010:Monte Carlo Method; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D011336:Probability; D011506:Proteins / Q000378:metabolism*; D014783:Viscosity
chemical_list: D008024:Ligands; D011506:Proteins