title: [Changes of miRNA-17-5p, miRNA-21 and miRNA-106a level during rat kidney ischemia-reperfusion injury].
abstract: OBJECTIVE
  Ischemia-reperfusion (I/R) is a main cause of acute kidney injury (AKI). The renal expression profiles of microRNA (miRNA) and time course of their changes after renal I/R were explored to screen acute AKI prognostic-related microRNAs and biomarkers.
  
  
  METHODS
  The expression profile of miRNA was analyzed for detecting miRNAs in kidney after renal I/R injury. Real-time polymerase chain reaction (PCR) was performed to validate the results of microarray. And the relationship was examined between kidney injury and time course of changes in selected miRNAs.
  
  
  RESULTS
  Twenty-one miRNAs were differentially expressed in kidney of rats with renal I/R injury. And 5 miRNAs had prominent differences. miR-17-5p, miR-21 and miR-106a were selected for further confirmation by quantitative real-time-PCR. And the results were consistent with those of microarry. During early stage (4 h) after I/R, the expression level of miR-17-5p significantly increased (P < 0.05). And it occurred earlier than those of BUN level and plasma concentration of neutrophil gelatinase-associated lipocalin (NGAL). Renal expressions of miR-21 and miR-106a were significantly elevated in ischemia 20 min and 30 min groups at 12 h and 24 h post-reperfusion (P < 0.01). And the trend was in accordance with those of BUN and NGAL.
  
  
  CONCLUSIONS
  miR-21, miR-17-5p and miR-106a are differentially expressed during different phases of renal I/R injury. And miR-17-5p is more sensitive than BUN and NGAL so that it is a more ideal biomarker for AKI.
authors.affiliation: Department of Intensive Care Unit, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Shuo
authors.initials: S
authors.lastname: Gu
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Zongyuan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Email: yu2796@163.com.
authors.forename: Xiangyou
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Urology, First Municipal People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, China; Email: huaianmjs@163.com.
authors.forename: Junsong
authors.initials: J
authors.lastname: Meng
mesh_terms: D058186:Acute Kidney Injury; D000209:Acute-Phase Proteins; D000818:Animals; D015415:Biomarkers; D007511:Ischemia; D000071068:Lipocalin-2; D054834:Lipocalins; D035683:MicroRNAs; D011379:Prognosis; D011518:Proto-Oncogene Proteins; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D015424:Reperfusion; D015427:Reperfusion Injury
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins