title: Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.
abstract: METHODS
  The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.
  
  
  RESULTS
  Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.
  
  
  CONCLUSIONS
  We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.
authors.affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Minker
authors.affiliation: Merck Millipore Bioscience Division, 78180 Saint-Quentin-en-Yvelines, France.
authors.forename: Livine
authors.initials: L
authors.lastname: Duban
authors.affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France ; Department of Medical Chemistry and Biochemistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Karas
authors.affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.
authors.forename: Päivi
authors.initials: P
authors.lastname: Järvinen
authors.affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.
authors.forename: Annelise
authors.initials: A
authors.lastname: Lobstein
authors.affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France ; UMR 7200 CNRS, Faculté de Pharmacie, 75 route du Rhin, 67400 Illkirch, France.
authors.forename: Christian D
authors.initials: CD
authors.lastname: Muller
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D029798:Blueberry Plants / Q000737:chemistry* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000633:toxicity; D029801:Vaccinium vitis-idaea / Q000737:chemistry* / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C491901:FAS protein, human; D010718:Phosphatidylserines; D010936:Plant Extracts; D044945:Proanthocyanidins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand; D019014:fas Receptor; D004247:DNA; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9