title: Transcription of non-classic major histocompatibility complex (MHC) class I in the bovine placenta throughout gestation and after Brucella abortus infection.
abstract: Transcription of non-classical major histocompatibility complex class I (MHC-I) was assessed in the bovine placenta throughout gestation. Additionally, the effect of Brucella abortus infection on expression of non-classical MHC-I was also evaluated using a chorioallantoic membrane explant model of infection. The non-classical MHC-I genes MICB and NC3 had higher levels of transcription in the intercotyledonary region when compared to the placentome, which had higher levels of transcription at the second trimester of gestation. NC1 and classical MHC-I had very low levels of transcription throughout gestation. Trophoblastic cells of B. abortus-infected chorioallantoic membrane explants had an increase in transcription of non-classical MHC-I at 4h post infection. Therefore, this study provides an analysis of non-classical MHC-I transcription at different stages of gestation and different placental tissues, and during B. abortus infection. These findings provide additional knowledge on immune regulation in placental tissues, a known immune-privileged site.
authors.affiliation: Mestrado em Ciência Animal, Universidade Estadual do Maranhão, São Luís, MA, Brazil.
authors.forename: Larissa Sarmento
authors.initials: LS
authors.lastname: Dos Santos
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Juliana Pinto
authors.initials: JP
authors.lastname: da Silva Mol
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Auricélio Alves
authors.initials: AA
authors.lastname: de Macedo
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Ana Patrícia Carvalho
authors.initials: AP
authors.lastname: Silva
authors.affiliation: Mestrado em Ciência Animal, Universidade Estadual do Maranhão, São Luís, MA, Brazil.
authors.forename: Diego Luiz
authors.initials: DL
authors.lastname: Dos Santos Ribeiro
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Renato Lima
authors.initials: RL
authors.lastname: Santos
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Tatiane Alves
authors.initials: TA
authors.lastname: da Paixão
authors.affiliation: Mestrado em Ciência Animal, Universidade Estadual do Maranhão, São Luís, MA, Brazil. Electronic address: alcinavcn@yahoo.com.
authors.forename: Alcina Vieira
authors.initials: AV
authors.lastname: de Carvalho Neta
mesh_terms: D000818:Animals; D002007:Brucellosis, Bovine / Q000150:complications / Q000235:genetics* / Q000276:immunology*; D002417:Cattle; D005260:Female; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D054502:Histocompatibility, Maternal-Fetal / Q000235:genetics; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I
keywords: Brucella abortus; Non-classical MHC-I; Placenta