title: Serology in the 21st century: the molecular-level analysis of the serum antibody repertoire.
abstract: The ensemble of antibodies found in serum and secretions represents the key adaptive component of B-cell mediated humoral immunity. The antibody repertoire is shaped by the historical record of exposure to exogenous factors such as pathogens and vaccines, as well as by endogenous host-intrinsic factors such as genetics, self-antigens, and age. Thanks to very recent technology advancements it is now becoming possible to identify and quantify the individual antibodies comprising the serological repertoire. In parallel, the advent of high throughput methods for antigen and immunosignature discovery opens up unprecedented opportunities to transform our understanding of numerous key questions in adaptive humoral immunity, including the nature and dynamics of serological memory, the role of polyspecific antibodies in health and disease and how protective responses to infections or vaccine challenge arise. Additionally, these technologies also hold great promise for therapeutic antibody and biomarker discovery in a variety of settings.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
authors.forename: Yariv
authors.initials: Y
authors.lastname: Wine
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Horton
authors.affiliation: Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Ippolito
authors.affiliation: Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA; Department of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA; Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX, USA. Electronic address: gg@che.utexas.edu.
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D000916:Antibody Diversity; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D056724:Immunity, Humoral; D057089:Pathology, Molecular; D040901:Proteomics; D012699:Serology / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens