title: Glucose Synthesis in a Protein-Based Artificial Photosynthesis System.
abstract: The objective of this study was to understand glucose synthesis of a protein-based artificial photosynthesis system affected by operating conditions, including the concentrations of reactants, reaction temperature, and illumination. Results from non-vesicle-based glyceraldehyde-3-phosphate (GAP) and glucose synthesis showed that the initial concentrations of ribulose-1,5-bisphosphate (RuBP) and adenosine triphosphate (ATP), lighting source, and temperature significantly affected glucose synthesis. Higher initial concentrations of RuBP and ATP significantly enhanced GAP synthesis, which was linearly correlated to glucose synthesis, confirming the proper functions of all catalyzing enzymes in the system. White fluorescent light inhibited artificial photosynthesis and reduced glucose synthesis by 79.2 % compared to in the dark. The reaction temperature of 40 °C was optimum, whereas lower or higher temperature reduced glucose synthesis. Glucose synthesis in the vesicle-based artificial photosynthesis system reconstituted with bacteriorhodopsin, F 0 F 1 ATP synthase, and polydimethylsiloxane-methyloxazoline-polydimethylsiloxane triblock copolymer was successfully demonstrated. This system efficiently utilized light-induced ATP to drive glucose synthesis, and 5.2 μg ml(-1) glucose was synthesized in 0.78-ml reaction buffer in 7 h. Light-dependent reactions were found to be the bottleneck of the studied artificial photosynthesis system.
authors.affiliation: Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC, 27695, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Yuan
authors.forename: Jack
authors.initials: J
authors.lastname: Zhou
authors.forename: Parkson Lee-Gau
authors.initials: PL
authors.lastname: Chong
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001436:Bacteriorhodopsins / Q000378:metabolism; D005947:Glucose / Q000096:biosynthesis*; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism; D008027:Light; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D010788:Photosynthesis / Q000528:radiation effects; D010940:Plant Proteins / Q000378:metabolism*; D017494:Proton Pumps; D012274:Ribulosephosphates / Q000378:metabolism; D013696:Temperature
chemical_list: D010940:Plant Proteins; D017494:Proton Pumps; D012274:Ribulosephosphates; D005986:Glyceraldehyde 3-Phosphate; C001933:ribulose-1,5 diphosphate; D001436:Bacteriorhodopsins; D000255:Adenosine Triphosphate; D025261:Mitochondrial Proton-Translocating ATPases; D005947:Glucose