title: Usefulness of Fluorescent Probe Prodan To Gain Insight into the Polarity of Plant Cuticles.
abstract: Plant cuticle is a complex mixture of hydrophobic components that controls the uptake of pesticides by plants. Although the transport of lipophilic molecules across the cuticle has been intensively studied, development of tools to measure the cuticle polarity has received little attention. We developed a rapid and simple analytical method to evaluate the polarity of cuticles in situ. This method uses Prodan, 6-propionyl-2-(dimethylaminonaphthalene), a medium-sensitive fluorescent probe. Tests on model surfaces with varied polarity (i.e., wax paraffin, polyethylene, C18) were carried out to test the feasibility of the measurement and to optimize the application of Prodan. Moreover, on the basis of the Kamlet-Taft solvatochromic comparison method, a relationship between the emission characteristics of Prodan and the number of carbon atoms in primary alcohols mimicking the solid medium was established. After optimization, the method was validated on three natural plant cuticles (leaf of Zamiifolia, skin of green pepper, and skin of white grape).
authors.affiliation: †Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Université Blaise Pascal, B.P. 10448, F-63000 Clermont-Ferrand, France.|§CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Stawinoga
authors.affiliation: †Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Université Blaise Pascal, B.P. 10448, F-63000 Clermont-Ferrand, France.|#ENSCCF, Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, B.P. 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Sleiman
authors.affiliation: †Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Université Blaise Pascal, B.P. 10448, F-63000 Clermont-Ferrand, France.|§CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Jeoffrey
authors.initials: J
authors.lastname: Chastain
authors.affiliation: †Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Université Blaise Pascal, B.P. 10448, F-63000 Clermont-Ferrand, France.|§CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Richard
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D029064:Araceae / Q000737:chemistry; D002212:Capsicum / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D027843:Vitis / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; C038517:prodan; D015081:2-Naphthylamine
keywords: Prodan; fluorescence; methodology; plant cuticle polarity