title: A Indole-Trizole-Rhodamine Triad as Ratiometric Fluorescent Probe for Nanomolar-Concentration Level Hg(2+) Sensing with High Selectivity.
abstract: A new type of ratiometric fluorescent probe capable of detecting Hg(2+) ions at nanomolar-concentration level with high selectivity was developed based on an indole-trizole-rhodamine triad and its practicability for intracellular Hg(2+) sensing was verified. The as-prepared fluorescent probe is capable of detecting Hg(2+) over other competing metal ions including Ag(+) with high selectivity. The synergistic effect of Hg(2+)-assisted conversion of the nonfluorescent ring-closed rhodamine moiety to the highly fluorescent ring-open form as well as the fluorescence signal amplification originating from the Förster resonance energy transfer (FRET) from indole-trizole conjugate to rhodamine moiety contributed to a detection limit of 11 nM of the probe for Hg(2+) sensing.
authors.affiliation: School of Chemistry & Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.|Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Chemistry & Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Chemistry, Renmin University of China, Beijing, 100872, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Chemistry & Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China. yuewang@ucas.ac.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Renmin University of China, Beijing, 100872, China. zilichen@ruc.edu.cn.
authors.forename: Zili
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Chemistry & Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China. zytian@ucas.ac.cn.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tian
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D061986:MCF-7 Cells; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D012235:Rhodamines; D014230:Triazoles; C030374:indole; D008628:Mercury
keywords: Energy transfer; Fluorescence bioimaging; Fluorescent probe; Mercury ions; Recognition selectivity