title: Effect of apolipoprotein A1 genetic polymorphisms on lipid profiles and the risk of coronary artery disease.
abstract: BACKGROUND
  The disorder of lipid metabolism and genetic predisposition are major risk factors for coronary artery disease (CAD). Variants in the apolipoprotein A1 (APOA1) gene play an important role in the regulation of lipids. The objective of the present study was to investigate the effect of two polymorphisms (-75 G/A and +83 C/T) of APOA1 on lipid profiles and the risk of CAD.
  
  
  METHODS
  A total number of 300 subjects with CAD and 300 age and sex matched healthy controls were enrolled for the study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing.
  
  
  RESULTS
  The frequencies of APOA1 -75 AA genotype [odds ratio (OR) =0.50, 95% confidence interval (CI) = 0.28, 0.88; P = 0.02] and APOA1 -75 A allele (OR =0.76, 95% CI = 0.59, 0.98; P = 0.04) were significantly lower in CAD than in controls. The APOA1 -75 A allele was significantly associated with increasing serum concentrations of ApoA1 and high-density lipoprotein cholesterol (HDL-C) (P < 0.001).
  
  
  CONCLUSIONS
  The individuals with the APOA1 -75 A allele were likely to have a lower risk of CAD as a result of its effect on higher serum concentrations of ApoA1 and HDL-C.
authors.affiliation: Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518000, Guangdong Province, China.
authors.forename: BiHong
authors.initials: B
authors.lastname: Liao
authors.affiliation: Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518000, Guangdong Province, China.
authors.forename: KeQi
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518000, Guangdong Province, China.
authors.forename: ShaoHong
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Cardiology, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518000, Guangdong Province, China.
authors.forename: HuaDong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017 East Gate Road, Shenzhen, 518000, Guangdong Province, China. zhengleixu@hotmail.com.
authors.forename: ZhengLei
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000235:genetics*; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D008055:Lipids