title: Inhibitory effect of Paeonia lactiflora Pallas extract (PE) on poly (I:C)-induced immune response of epidermal keratinocytes.
abstract: Epidermal keratinocytes provide protective role against external stimuli by barrier formation. In addition, kertinocytes exerts their role as the defense cells via activation of innate immunity. Disturbance of keratinocyte functions is related with skin disorders. Psoriasis is a common skin disease related with inflammatory reaction in epidermal cells. We attempted to find therapeutics for psoriasis, and found that Paeonia lactiflora Pallas extract (PE) has an inhibitory potential on poly (I:C)-induced inflammation of keratinocytes. PE significantly inhibited poly (I:C)-induced expression of crucial psoriatic cytokines, such as IL-6, IL-8, CCL20 and TNF-α, via down-regulation of NF-κB signaling pathway in human keratinocytes. In addition, PE significantly inhibited poly (I:C)-induced inflammasome activation, in terms of IL-1β and caspase-1 secretion. Finally, PE markedly inhibited poly (I:C)-increased NLRP3, an important component of inflammasome. These results indicate that PE has an inhibitory effect on poly (I:C)-induced inflammatory reaction of keratinocytes, suggesting that PE can be developed for the treatment of psoriasis.
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Mi-Ra
authors.initials: MR
authors.lastname: Choi
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Dae-Kyoung
authors.initials: DK
authors.lastname: Choi
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Kyung-Cheol
authors.initials: KC
authors.lastname: Sohn
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Lim
authors.affiliation: Department of Obstetrics & Gynecology, College of Korean Medicine, Dongguk University Seoul, Korea.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Kim
authors.affiliation: Department of Anatomy, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Myung
authors.initials: M
authors.lastname: Im
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Seo
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea.
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University Daejeon, Korea ; Skin Med Company Daejeon, Korea.
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D003879:Dermatologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004817:Epidermis / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D058847:Inflammasomes / Q000187:drug effects / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D029596:Paeonia / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011070:Poly I-C / Q000494:pharmacology*; D011565:Psoriasis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D002352:Carrier Proteins; D016207:Cytokines; D003879:Dermatologic Agents; D058847:Inflammasomes; D018836:Inflammation Mediators; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D010936:Plant Extracts; D011070:Poly I-C
keywords: Keratinocytes; NF-κB; Paeonia lactiflora Pallas extract; inflammasome; poly (I:C)