title: Maternal hypertension programs increased cerebral tissue damage following stroke in adult offspring.
abstract: The maternal system is challenged with many physiological changes throughout pregnancy to prepare the body to meet the metabolic needs of the fetus and for delivery. Many pregnancies, however, are faced with pathological stressors or complications that significantly impact maternal health. A shift in this paradigm is now beginning to investigate the implication of pregnancy complications on the fetus and their continued influence on offspring disease risk into adulthood. In this investigation, we sought to determine whether maternal hypertension during pregnancy alters the cerebral response of adult offspring to acute ischemic stroke. Atrial natriuretic peptide gene-disrupted (ANP(-/-)) mothers exhibit chronic hypertension that escalates during pregnancy. Through comparison of heterozygote offspring born from either normotensive (ANP(+/-WT)) or hypertensive (ANP(+/-KO)) mothers, we have demonstrated that offspring exposed to maternal hypertension exhibit larger cerebral infarct volumes following middle cerebral artery occlusion. Observation of equal baseline cardiovascular measures, cerebrovascular structure, and cerebral blood volumes between heterozygote offspring suggests no added influences on offspring that would contribute to adverse cerebral response post-stroke. Cerebral mRNA expression of endothelin and nitric oxide synthase vasoactive systems demonstrated up-regulation of Et-1 and Nos3 in ANP(+/-KO) mice and thus an enhanced acute vascular response compared to ANP(+/-WT) counterparts. Gene expression of Na(+)/K(+) ATPase channel isoforms, Atp1a1, Atp1a3, and Atp1b1, displayed no significant differences. These investigations are the first to demonstrate a fetal programming effect between maternal hypertension and adult offspring stroke outcome. Further mechanistic studies are required to complement epidemiological evidence of this phenomenon in the literature.
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. n.ventura@queensu.ca.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Ventura
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. ayj@queensu.ca.|Department of Medicine (Neurology), Kingston General Hospital, Kingston, ON, Canada. ayj@queensu.ca.
authors.forename: Albert Y
authors.initials: AY
authors.lastname: Jin
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. myt@queensu.ca.
authors.forename: M Yat
authors.initials: MY
authors.lastname: Tse
authors.affiliation: Department of Medicine (Neurology), Kingston General Hospital, Kingston, ON, Canada. nicki.peterson@queensu.ca.
authors.forename: Nichole T
authors.initials: NT
authors.lastname: Peterson
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. andrewd@queensu.ca.|Centre for Neuroscience, Queen's University, Kingston, ON, Canada. andrewd@queensu.ca.
authors.forename: R David
authors.initials: RD
authors.lastname: Andrew
authors.affiliation: Cancer Research Institute Queen's University, Kingston, ON, Canada. mewburnj@queensu.ca.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Mewburn
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. pangsc@post.queensu.ca.
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Pang
mesh_terms: D032721:Adult Children; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D002544:Cerebral Infarction / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D016232:Endothelins / Q000235:genetics; D005260:Female; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000235:genetics*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000235:genetics; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology / Q000235:genetics / Q000473:pathology*
chemical_list: D016232:Endothelins; D009320:Atrial Natriuretic Factor; D019001:Nitric Oxide Synthase
keywords: Endothelin; Fetal programming; Hypertension; Na+/K+-ATPase; Natriuretic peptide; Nitric oxide synthase; Pregnancy; Stroke