title: Expression Level of Genes Coding for Cell Adhesion Molecules of Cadherin Group in Colorectal Cancer Patients.
abstract: BACKGROUND
  Colorectal Cancer (CRC) is one of the most frequently diagnosed neoplasms and also one of the main death causes. Cell adhesion molecules are taking part in specific junctions, contributing to tissue integrality. Lower expression of the cadherins may be correlated with poorer differentiation of the CRC, and its more aggressive phenotype. The aim of the study is to designate the cadherin genes potentially useful for the diagnostics, prognostics, and the treatment of CRC.
  
  
  METHODS
  Specimens were collected from 28 persons (14 female and 14 male), who were operated for CRC. The molecular analysis was performed using oligonucleotide microarrays, mRNA used was collected from adenocarcinoma, and macroscopically healthy tissue. The results were validated using qRT-PCR technique.
  
  
  RESULTS
  Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression. The control group was divided into 2 groups, the one was appropriate control (C1), the second (C2) had the genetic properties of the CRC, without pathological changes histologically and macroscopically. The other 2 groups were: LSC (Low stage cancer) and HSC (High stage cancer). Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue.
  
  
  CONCLUSIONS
  The levels of genes expression are different for several groups of cadherins, and are related with the stage of CRC, therefore could be potentially the useful marker of the stage of the disease, also applicable in treatment and diagnostics of CRC.
authors.affiliation: Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Lorenc
authors.affiliation: Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: Mieszko Norbert
authors.initials: MN
authors.lastname: Opiłka
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Katowice, Poland.
authors.forename: Celina
authors.initials: C
authors.lastname: Kruszniewska-Rajs
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Katowice, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Rajs
authors.affiliation: Department of Propedeutics Surgery, Chair of General, Colorectal and Polytrauma Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Waniczek
authors.affiliation: Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Starzewska
authors.affiliation: Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Lorenc
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Katowice, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
mesh_terms: D015820:Cadherins / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules