title: A novel "dual-potential" electrochemiluminescence aptasensor array using CdS quantum dots and luminol-gold nanoparticles as labels for simultaneous detection of malachite green and chloramphenicol.
abstract: A novel type of "dual-potential" electrochemiluminescence (ECL) aptasensor array was fabricated on a homemade screen-printed carbon electrode (SPCE) for simultaneous detection of malachite green (MG) and chloramphenicol (CAP) in one single assay. The SPCE substrate consisted of a common Ag/AgCl reference electrode, carbon counter electrode and two carbon working electrodes (WE1 and WE2). In the system, CdS quantum dots (QDs) were modified on WE1 as cathode ECL emitters and luminol-gold nanoparticles (L-Au NPs) were modified on WE2 as anode ECL emitters. Then the MG aptamer complementary strand (MG cDNA) and CAP aptamer complementary strand (CAP cDNA) were attached on CdS QDs and L-Au NPs, respectively. The cDNA would hybridize with corresponding aptamer that was respectively tagged with cyanine dye (Cy5) (as quenchers of CdS QDs) and chlorogenic acid (CA) (as quenchers of l-Au NPs) using poly(ethylenimine) (PEI) as a bridging agent. PEI could lead to a large number of quenchers on the aptamer, which increased the quenching efficiency. Upon MG and CAP adding, the targets could induce strand release due to the highly affinity of analytes toward aptamers. Meanwhile, it could release the Cy5 and CA, which recovered cathode ECL of CdS QDs and anode ECL of L-Au NPs simultaneously. This "dual-potential" ECL strategy could be used to detect MG and CAP with the linear ranges of 0.1-100 nM and 0.2-150 nM, with detection limits of 0.03 nM and 0.07 nM (at 3sB), respectively. More importantly, this designed method was successfully applied to determine MG and CAP in real fish samples and held great potential in the food analysis.
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Feng
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China; School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China.
authors.forename: Huairong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yan
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Li
authors.affiliation: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Faculty of Marine, Ningbo University, Ningbo 315211, PR China.
authors.forename: Futao
authors.initials: F
authors.lastname: Hu
authors.affiliation: Faculty of Marine, Ningbo University, Ningbo 315211, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry*; D002701:Chloramphenicol / Q000032:analysis*; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry; D008839:Microelectrodes; D045663:Quantum Dots; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000032:analysis*; D018036:Selenium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D045424:Complex Mixtures; D012394:Rosaniline Dyes; D018036:Selenium Compounds; C005095:malachite green; D008165:Luminol; D002701:Chloramphenicol; C058667:cadmium selenide
keywords: Electrochemiluminescent aptasensor array; Malachite green and chloramphenicol; Quenching probes; Simultaneous detection