title: Soybean isoflavone treatment induces osteoblast differentiation and proliferation by regulating analysis of Wnt/β-catenin pathway.
abstract: It has been reported that soybean isoflavones (SI) have anti-bone resorptive activity in vivo. However, little is known about the cellular and molecular mechanisms of this effect. In this study, we investigated the effects of SI on osteoblast differentiation and proliferation. Results showed that SI promoted osteoblast proliferation and differentiation which could be marked as the formation of hydroxyapatite crystals or extracellular matrix and increased ALP level. SI treatment markedly decreased RANKL levels and increased OPG levels in osteoblast cells. SI resulted in activation of the Wnt/β-catenin pathway. These results demonstrate that SI may be a useful remedy for the treatment of osteoporosis.
authors.affiliation: The Second Hospital of Dalian Medical University, Dalian 116023, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.affiliation: Orthopedic Department, Changchun University of Traditional Chinese Medicine Affiliated Hospital, Changchun 130021, People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Hands and Feet Microsurgery, The Second Hospital of Dalian Medical University, Dalian 116023, People's Republic of China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Hands and Feet Microsurgery, The Second Hospital of Dalian Medical University, Dalian 116023, People's Republic of China.
authors.forename: Zhengnan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Hands and Feet Microsurgery, The Second Hospital of Dalian Medical University, Dalian 116023, People's Republic of China. Electronic address: lianghddlf@sina.com.
authors.forename: Haidong
authors.initials: H
authors.lastname: Liang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D007529:Isoflavones / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D053244:Osteoprotegerin / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D013025:Glycine max / Q000737:chemistry; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D060449:Wnt Signaling Pathway; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C495268:Ctnnb1 protein, rat; D007529:Isoflavones; D053244:Osteoprotegerin; D010936:Plant Extracts; D053245:RANK Ligand; C506032:Tnfrsf11b protein, rat; D051153:Wnt Proteins; D060509:Wnt3A Protein; C532827:Wnt7b protein, rat; D051176:beta Catenin; D000469:Alkaline Phosphatase
keywords: RANKL; Soybean isoflavones; Wnt/β-catenin