title: Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface.
abstract: Glucose oxidase (GOx) and horseradish peroxidase (HRP) were co-immobilized to the polyacrylicacid block of a poly(acrylicacid-b-4-cyanobiphenyl-4'-undecylacrylate) (PAA-b-LCP) copolymer in water. PAA-b-LCP was strongly anchored by the LCP block in 4-cyano-4'-pentylbiphenyl (5CB) which was contained in a transmission electron microscope (TEM) grid for glucose detection. The optimal conditions for the performance of the TEM grid glucose biosensor were studied in terms of the activity and stability of the immobilized enzymes. Glucose in water was detected by the 5CB changing from a planar to a homeotropic orientation, as observed through a polarized optical microscope. The TEM biosensor detected glucose concentrations at ⩾0.02 mM, with an optimal GOx/HRP molar ratio of 3/1. This glucose biosensor has characteristics of enzyme sensitivity and stability, reusability, the ease and selective glucose detection which may provide a new way of detecting glucose.
authors.affiliation: Department of Polymer Science and Engineering, School of Applied Chemical Engineering, #1370 Sangyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea.
authors.forename: Mashooq
authors.initials: M
authors.lastname: Khan
authors.affiliation: Department of Polymer Science and Engineering, School of Applied Chemical Engineering, #1370 Sangyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea. Electronic address: psy@knu.ac.kr.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Park
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D050866:Liquid Crystals / Q000737:chemistry*; D014867:Water / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D014867:Water; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase
keywords: Bienzyme; Biosensor; Glucose; Glucose oxidase; Liquid crystal; Peroxidase