title: Value of counting positive PHH3 cells in the diagnosis of uterine smooth muscle tumors.
abstract: The diagnosis of uterine smooth muscle tumors including leiomyosarcomas (LMS), smooth muscle tumors of uncertain malignant potential (STUMP), bizarre (atypical) leiomyoma (BLM), mitotically active leiomyoma (MAL) and leiomyoma (LM) depends on a combination of microscopic features, such as mitoses, cytologic atypia, and coagulative tumor cell necrosis. However, a small number of these tumors still pose difficult diagnostic challenges. The assessment of accurate mitotic figures (MF) is one of the major parameters in the proper classification of uterine smooth muscle tumors. This assessment can be hampered by the presence of increased number of apoptotic bodies or pyknotic nuclei, which frequently mimic mitoses. Phospho-histone H3 (PHH3) is a recently described immunomarker specific for cells undergoing mitoses. In our study, we collected 132 cases of uterine smooth muscle tumors, including 26 LMSs, 16 STUMPs, 30 BLMs, 30 MALs and 30 LMs. We used mitosis specific marker PHH3 to count mitotic indexes (MI) of uterine smooth muscle tumors and compared with the mitotic indexes of hematoxylin and eosin (H&E). There is a positive correlation with the number of mitotic figures in H&E-stained sections and PHH3-stained sections (r=0.944, P<0.05). The ratio of PHH3-MI to H&E-MI has no statistically significant difference in each group except for LMs (P>0.05). The counting value of PHH3 in LMSs have significantly higher than STUMPs, BLMs, MALs and LMs (P<0.001) and the counting value of PHH3 is 1.5±0.5 times of the number of mitotic indexes in H&E. To conclude, our results show that counting PHH3 is a useful index in the diagnosis of uterine smooth muscle tumors and it can provide a more accurate index instead of the time-honored mitotic figure counts at a certain ratio.
authors.affiliation: Department of Gynecology and Obstetrics, Tianjin Central Clinical College, Tianjin Medical University Tianjin, China ; Department of Pathology, Tianjin Central Hospital of Gynecology and Obstetrics Tianjin, China.
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Pang
authors.affiliation: Department of Gynecology and Obstetrics, Tianjin Central Clinical College, Tianjin Medical University Tianjin, China.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Tianjin Central Clinical College, Tianjin Medical University Tianjin, China ; Department of Pathology, Tianjin Central Hospital of Gynecology and Obstetrics Tianjin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Xiangya Medical School, Central South University Changsha, Hunan, China ; Houston Methodist Research Institute Houston, Texas, USA.
authors.forename: Yian-Zhu
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Department of Pathology, Tianjin Central Hospital of Gynecology and Obstetrics Tianjin, China.
authors.forename: Yi-Quan
authors.initials: YQ
authors.lastname: Shi
authors.affiliation: Department of Gynecology and Obstetrics, Tianjin Central Clinical College, Tianjin Medical University Tianjin, China ; Department of Pathology, Tianjin Central Hospital of Gynecology and Obstetrics Tianjin, China.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006657:Histones / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000175:diagnosis*; D007890:Leiomyosarcoma / Q000175:diagnosis*; D008875:Middle Aged; D008940:Mitotic Index; D010766:Phosphorylation; D012189:Retrospective Studies; D014594:Uterine Neoplasms / Q000175:diagnosis*
chemical_list: D014408:Biomarkers, Tumor; D006657:Histones
keywords: PHH3; Uterine smooth muscle tumors; immunohistochemical; mitotic index