title: Stress-Responsive Expression, Subcellular Localization and Protein-Protein Interactions of the Rice Metacaspase Family.
abstract: Metacaspases, a class of cysteine-dependent proteases like caspases in animals, are important regulators of programmed cell death (PCD) during development and stress responses in plants. The present study was focused on comprehensive analyses of expression patterns of the rice metacaspase (OsMC) genes in response to abiotic and biotic stresses and stress-related hormones. Results indicate that members of the OsMC family displayed differential expression patterns in response to abiotic (e.g., drought, salt, cold, and heat) and biotic (e.g., infection by Magnaporthe oryzae, Xanthomonas oryzae pv. oryzae and Rhizoctonia solani) stresses and stress-related hormones such as abscisic acid, salicylic acid, jasmonic acid, and 1-amino cyclopropane-1-carboxylic acid (a precursor of ethylene), although the responsiveness to these stresses or hormones varies to some extent. Subcellular localization analyses revealed that OsMC1 was solely localized and OsMC2 was mainly localized in the nucleus. Whereas OsMC3, OsMC4, and OsMC7 were evenly distributed in the cells, OsMC5, OsMC6, and OsMC8 were localized in cytoplasm. OsMC1 interacted with OsLSD1 and OsLSD3 while OsMC3 only interacted with OsLSD1 and that the zinc finger domain in OsMC1 is responsible for the interaction activity. The systematic expression and biochemical analyses of the OsMC family provide valuable information for further functional studies on the biological roles of OsMCs in PCD that is related to abiotic and biotic stress responses.
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. leihero2008@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. zhanghj82@zju.edu.cn.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. yongbohong@126.com.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Hong
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. liurui90913@163.com.
authors.forename: Shixia
authors.initials: S
authors.lastname: Liu
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. dyli@zju.edu.cn.
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. fmsong@zju.edu.cn.
authors.forename: Fengming
authors.initials: F
authors.lastname: Song
mesh_terms: D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020082:Magnaporthe / Q000187:drug effects / Q000502:physiology; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology; D011401:Promoter Regions, Genetic / Q000235:genetics; D025941:Protein Interaction Mapping; D021381:Protein Transport / Q000187:drug effects; D012232:Rhizoctonia / Q000187:drug effects / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D013696:Temperature; D014974:Xanthomonas / Q000187:drug effects / Q000502:physiology
chemical_list: D010937:Plant Growth Regulators; D012965:Sodium Chloride; D020169:Caspases
keywords: abiotic and biotic stress; expression patterns; metacaspase; protein–protein interaction; rice; subcellular localization