title: [Effects of different fibrin glue combination modes on the proliferation and viability of dental follicle cells].
abstract: OBJECTIVE
  This study explores the effects of different fibrin glue combination modes on the survival, proliferation, and apoptosis of dental follicle cells (DFCs), as well as to evaluate the feasibility and effectiveness of fibrin glue as transplantation material.
  
  
  METHODS
  The membranes of surviving DFCs were marked using 3,3'-dioctadecyloxa carbocyanine perchlorate (DIO), and the cell number was counted by using ImageJ2x software. The apoptotic cells were marked with prodium iodide (PI).
  
  
  RESULTS
  Compared with that of the 3D-2 and 2D-1 groups, the degradation speed of the 3D-1 group was the slowest. DFCs could survive and grow well in fibrinogen with a concentration of 15 mg · mL⁻¹ supplemented with thrombin with a concentration of 2 U · mL⁻¹. In particular, the 3D-1 combination mode was significantly conducive to cell proliferation and stretching.
  
  
  CONCLUSIONS
  Fibrin glue can be used as an effective cell transplantation material. The different combination modes have certain effects on cell proliferation. The 3D-1 combination mode is more conducive to the survival and proliferation of DFCs than other modes.
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Yang
authors.initials: Y
authors.lastname: Chao
authors.forename: Chen
authors.initials: C
authors.lastname: Guoqing
authors.forename: Tian
authors.initials: T
authors.lastname: Weidong
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival; D003795:Dental Sac / Q000166:cytology*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D005340:Fibrinogen; D006801:Humans; D013917:Thrombin
chemical_list: D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen; D013917:Thrombin