title: Correlation Analysis of Nasopharyngeal Carcinoma TNM Staging with Serum EA IgA and VCA IgA in EBV and VEGF-C and -D.
abstract: BACKGROUND
  Nasopharyngeal carcinoma often occurs in humans in the nasopharyngeal epithelium area. Ebstein-Barr (EB) virus plays a key role in the process of nasopharyngeal carcinoma lesions. Early antigen antibody (EA-IgA) and viral capsid antigen IgA (VCA-IgA) of EB virus detection in serum can effectively monitor the process of nasopharyngeal carcinoma lesions. Serum vascular endothelial growth factor (VEGF) -C and VEGF-D expression detection can reflect the distant metastases ability of human tumor cells.
  
  
  METHODS
  153 cases of nasopharyngeal carcinoma patients in our hospital were enrolled, while 148 cases of healthy adults were selected as control. ELISA was used to detect serum EA-IgA, VCA-IgA, VEGF-C and -D expression levels. Spearman rank correlation analysis was applied to test the correlation of nasopharyngeal carcinoma TNM clinical stage and different indexes.
  
  
  RESULTS
  Serum EA-IgA, VCA-IgA, VEGF-C and -D expression in nasopharyngeal carcinoma patients was 43.74±2.6 U•mL^-1, 62.5±2.7 U·mL^-1, 473.25±3.4 pg•mL^-1, and 498.36±2.3 pg•mL^-1, respectively, which was significantly higher than in the control group as 18.65±3.7 U•mL^-1, 23.74±1.5 U•mL^-1, 225.42±2.3 pg•mL^-1, and 257.24±3.5 pg•mL^-1 (P<0.05). Nasopharyngeal carcinoma TNM clinical staging was obviously correlated with serum EA-IgA, VCA-IgA, and VEGF-C (P<0.05), but not VEGF-D (P>0.05).
  
  
  CONCLUSIONS
  Nasopharyngeal carcinoma patient serum EA-IgA and VCA-IgA expression levels were significantly correlated with TNM staging. The high levels of these 3 indicators suggest advanced nasopharyngeal carcinoma TNM staging and serious lesions.
authors.affiliation: Department of Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).
authors.forename: Ruimei
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood; D002277:Carcinoma; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000276:immunology / Q000473:pathology; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D037181:Lectins, C-Type / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000097:blood* / Q000473:pathology / Q000821:virology*; D009367:Neoplasm Staging; D042582:Vascular Endothelial Growth Factor C / Q000097:blood*; D042643:Vascular Endothelial Growth Factor D / Q000097:blood*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral; D014408:Biomarkers, Tumor; D007070:Immunoglobulin A; D037181:Lectins, C-Type; D042582:Vascular Endothelial Growth Factor C; D042643:Vascular Endothelial Growth Factor D; D000099192:CD69 Antigens; C467486:VEGFC protein, human; C579459:VEGFD protein, human