title: Improved Oocyte Isolation and Embryonic Development of Outbred Deer Mice.
abstract: In this study, we improved the protocol for isolating cumulus-oocyte complexes (COCs) from the outbred deer mice by using only one hormone (instead of the widely used combination of two hormones) with reduced dose. Moreover, we identified that significantly more metaphase II (MII) oocytes could be obtained by supplementing epidermal growth factor (EGF) and leukemia inhibition factor (LIF) into the previously established medium for in vitro maturation (IVM) of the COCs. Furthermore, we overcame the major challenge of two-cell block during embryonic development of deer mice after either in vitro fertilization (IVF) or parthenogenetic activation (PA) of the MII oocytes, by culturing the two-cell stage embryos on the feeder layer of inactivated mouse embryonic fibroblasts (MEFs) in the medium of mouse embryonic stem cells. Collectively, this work represents a major step forward in using deer mice as an outbred animal model for biomedical research on reproduction and early embryonic development.
authors.affiliation: 1] Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210. [2] Davis Heart and Lung Research Institute, The Ohio State University,Columbus, OH 43210.
authors.forename: Jung Kyu
authors.initials: JK
authors.lastname: Choi
authors.affiliation: 1] Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210. [2] Davis Heart and Lung Research Institute, The Ohio State University,Columbus, OH 43210. [3] James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: He
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D006080:Ovarian Follicle / Q000166:cytology; D010053:Ovary / Q000166:cytology; D010060:Ovulation / Q000187:drug effects; D010542:Peromyscus
chemical_list: D053605:Leukemia Inhibitory Factor; D004815:Epidermal Growth Factor