title: Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide.
abstract: This study aimed to establish a hormonal induction protocol for spermiation of Brycon cephalus males, using Ala6, Pro9Net-mGnRH + metoclopramide (Ovopel®). Thus, 20 males were used divided into three inductor treatments [⅓ pellet/kg (T1), ⅔ pellet/kg (T2) and 1⅓ pellet/kg (T3)] and one control group (CO), which only received physiological solution applications (0.9% NaCl). All treatments were applied in a single dose. For evaluation of the availability of the treatment, the following seminal parameters were analyzed: seminal volume, subjective spermatic motility, duration of motility, pH, osmolality and spermatic concentration. T3 showed the highest seminal volume (4.66 ± 1.52 ml), and was significantly different in comparison with T1 (2.0 ± 0.9 ml), T2 (3.5 ± 1.3 ml) and CO (2.3 ± 1.2 ml). In relation to spermatic motility, T2 and T3 showed significantly higher levels [5, (81-100%)]. However, T3 showed significantly lower average sperm motility duration than T1, T2 and CO (30 ± 7 s; 28 ± 6 s; 32 ± 8 s, respectively). With regard to the seminal parameters of spermatic concentration, pH and osmolality, no significant variation was verified among treatments. In conclusion, mGnRH + metoclopramide used for hormonal induction of B. cephalus reproduction does not induce changes related to spermatic concentration, pH and osmolality parameters of the seminal fluid and the most adequate doses among tested treatments were ⅔ pellet/kg live fish.
authors.affiliation: Laboratório de Ictiologia Neotropical - LINEO,Depto de Biologia e Zootecnia,FEIS/UNESP - Universidade Estadual Paulista 'Júlio de Mesquita Filho',Rua Monção,226 - Zona Norte 15385-000 - Ilha Solteira,São Paulo,Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Bashiyo-Silva
authors.affiliation: Laboratório de Ictiologia Neotropical - LINEO,Depto de Biologia e Zootecnia,FEIS/UNESP - Universidade Estadual Paulista 'Júlio de Mesquita Filho',Rua Monção,226 - Zona Norte 15385-000 - Ilha Solteira,São Paulo,Brazil.
authors.forename: Raphael da Silva
authors.initials: Rda S
authors.lastname: Costa
authors.affiliation: Laboratório de Ictiologia Neotropical - LINEO,Depto de Biologia e Zootecnia,FEIS/UNESP - Universidade Estadual Paulista 'Júlio de Mesquita Filho',Rua Monção,226 - Zona Norte 15385-000 - Ilha Solteira,São Paulo,Brazil.
authors.forename: Douglas de Castro
authors.initials: Dde C
authors.lastname: Ribeiro
authors.affiliation: Centro Nacional de Pesquisa e Conservação de Peixes Continentais (CEPTA/ICMBio),Rodovia SP-201 (Pref. Euberto Nemésio Pereira de Godoy),Km 6.5,13630-970 - Pirassununga,São Paulo,Brazil.
authors.forename: José Augusto
authors.initials: JA
authors.lastname: Senhorini
authors.affiliation: Laboratório de Ictiologia Neotropical - LINEO,Depto de Biologia e Zootecnia,FEIS/UNESP - Universidade Estadual Paulista 'Júlio de Mesquita Filho',Rua Monção,226 - Zona Norte 15385-000 - Ilha Solteira,São Paulo,Brazil.
authors.forename: Rosicleire
authors.initials: R
authors.lastname: Veríssimo-Silveira
authors.affiliation: Department of Biology and Zootechny,FEIS/UNESP - State University of São Paulo 'Júlio de Mesquita Filho',Rua Monção,226,Zona Norte,Ilha Solteira,SP,15385-000,Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Ninhaus-Silveira
mesh_terms: D000818:Animals; D060125:Characiformes / Q000502:physiology*; D065127:Dopamine D2 Receptor Antagonists / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008787:Metoclopramide / Q000494:pharmacology*; D009994:Osmolar Concentration; D012661:Semen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013997:Time Factors
chemical_list: D065127:Dopamine D2 Receptor Antagonists; D007987:Gonadotropin-Releasing Hormone; D008787:Metoclopramide
keywords: Induced spawning; Neotropical fish; Semen quality; Seminal characteristics