title: Granulocyte-colony stimulating factor as a treatment for diabetic neuropathy in rat.
abstract: Effective treatment of diabetic neuropathy (DN) remains unsolved. We serendipitously observed dramatic relief of pain in several patients with painful DN receiving granulocyte-colony stimulating factor (G-CSF). The aim of this study was to determine if G-CSF could treat DN in an animal model and to ascertain its mechanism of action. In a rodent model of DN, G-CSF dramatically recovered nerve function, retarded histological nerve changes and increased the expression of neurotrophic factors within nerve. A sex-mismatched bone marrow transplantation (BMT) study revealed that G-CSF treatment increased the abundance of bone marrow (BM)-derived cells in nerves damaged by DN. However, we did not observe evidence of transdifferentiation or cell fusion of BM-derived cells. The beneficial effects of G-CSF were dependent on the integrity of BM. In conclusion, G-CSF produced a therapeutic effect in a rodent model of DN, which was attributed, at least in part, to the actions of BM-derived cells.
authors.affiliation: Division of Cardiology, Hanyang University College of Medicine, Seoul, 133-792, South Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 133-792, South Korea. Electronic address: kskim@hanyang.ac.kr.
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 133-792, South Korea.
authors.forename: Yi-Sun
authors.initials: YS
authors.lastname: Song
authors.affiliation: Division of Cardiology, Yanbian University, Yanji, 133000, China.
authors.forename: Jiyong
authors.initials: J
authors.lastname: Jin
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 133-792, South Korea.
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Joe
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 133-792, South Korea.
authors.forename: Byung-Im
authors.initials: BI
authors.lastname: So
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 133-792, South Korea.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: Division of Cardiology, Yanbian University, Yanji, 133000, China.
authors.forename: Cheng-Hu
authors.initials: CH
authors.lastname: Fang
authors.affiliation: Department of Rehabilitation Medicine, Hanyang University College of Medicine, Seoul, 133-792, South Korea.
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Medical Genetics, Hanyang University, Seoul, 133-792, South Korea.
authors.forename: Youl-Hee
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Department of Anatomy and Cell Biology, Hanyang University, Seoul, 133-792, South Korea.
authors.forename: Sejin
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Department of Dermatology, Hanyang University College of Medicine, Seoul, 133-792, South Korea.
authors.forename: Young-Suck
authors.initials: YS
authors.lastname: Ro
authors.affiliation: Department of Cardiovascular Surgery, Hanyang University College of Medicine, Seoul, 133-792, South Korea.
authors.forename: Hyuck
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Endocrinology & Metabolism, Hanyang University College of Medicine, Seoul, 133-792, South Korea.
authors.forename: You-Hern
authors.initials: YH
authors.lastname: Ahn
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, VU Station B, Box 351631 Nashville, TN 37235, USA.
authors.forename: Hak-Joon
authors.initials: HJ
authors.lastname: Sung
authors.affiliation: Department of Neurology, Seoul National University College of Medicine, Seoul, 110-744, South Korea.
authors.forename: Jung-Joon
authors.initials: JJ
authors.lastname: Sung
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, 110-744, South Korea.
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Park
authors.affiliation: Del E. Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford-Burnham Medical Research Institute, CA, USA; Department of Neurosciences, School of Medicine, University of California at San Diego, CA, USA. Electronic address: slipton@ucsd.edu.
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Lipton
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D016026:Bone Marrow Transplantation; D003929:Diabetic Neuropathies / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D008297:Male; D009414:Nerve Growth Factors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D009414:Nerve Growth Factors; D016179:Granulocyte Colony-Stimulating Factor
keywords: Bone marrow-derived cells; Diabetic neuropathy; Granulocyte-colony stimulating factor