title: RETINOID METABOLISM AND CONVERSION OF RETINOL TO DEHYDRORETINOL IN THE CRAYFISH (PROCAMBARUS CLARKII) RETINA.
abstract: Activities of retinylester hydrolase (REHase) and retinol dehydrogenase (RDHase) were detected in the crayfish retina which has two visual pigment chromophores and three stored retinoids.RDHase and 11-cisREHase activities were localized to rhabdomal fraction and enzymes were specific to 11-cisisomers of substrates. Overall reaction from retinylester to retinal was highly specific to 11-cisisomers and this reaction was not selective between retinol and dehydroretinol.When 11-cis[3H]-retinol was incubated with retina homogenates, radioactivity was detected in 11-cisdehydroretinol.These results suggest that retinol is converted to dehydroretinol after hydrolysis of retinylesters.
authors.affiliation: Department of Pharmacology, Hyogo College of Medicine, Hyogo 663, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzsuki
authors.affiliation: Photodynamics Research Center, Institute of Physical and Chemical Research, Miyagi 981, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Terakita
authors.affiliation: Division of Life Sciences, The University of Texas at San Antonio, San Antonio, TX 78249, U.S.A.
authors.forename: Andrew T C
authors.initials: AT
authors.lastname: Tsin