title: External Quality Assessment of Molecular Detection of Ebola Virus in China.
abstract: In 2014, Ebola hemorrhagic fever broke out in West Africa. As contact between China and West Africa is frequent, the possibility that Ebola virus would enter China was high. Thus, an external assessment of the quality of Ebola virus detection was organized by the National Center for Clinical Laboratories in China. Virus-like particles encapsulating known sequences of epidemic strains of Ebola virus from 2014 were prepared as positive quality controls. The sample panel, which was composed of seven positive and three negative samples, was dispatched to 19 laboratories participating in this assessment of Ebola virus detection. Accurate detection was reported at 14 of the 19 participating laboratories, with a sensitivity of 91.43% and a specificity of 100%. Four participants (21.05%) reported false-negative results and were classified as "acceptable." One participant (5.26%) did not detect any positive samples and was thus classified as "improvable." Based on the results returned, the ability to detect weakly positive Ebola specimens should be improved. Furthermore, commercial assays and the standard primers offered by the Chinese Centers for Disease Control and Prevention were found to be most accurate and dependable for Ebola detection. A two-target detection approach is recommended for Ebola screening; this approach could reduce the probability of false-negative results. Additionally, standardization of operations and punctual adjustment of instruments are necessary for the control and prevention of Ebola virus.
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Guojing
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Jia
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Mingju
authors.initials: M
authors.lastname: Hao
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Le
authors.initials: L
authors.lastname: Chang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Fifth School of Clinical Medicine, Peking University, Beijing, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Guigao
authors.initials: G
authors.lastname: Lin
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Rongxue
authors.initials: R
authors.lastname: Peng
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Li
mesh_terms: D002681:China; D017931:DNA Primers / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D029043:Ebolavirus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007753:Laboratories / Q000592:standards; D011785:Quality Assurance, Health Care
chemical_list: D017931:DNA Primers; D015342:DNA Probes