title: Identification of ecotype-specific marker genes for categorization of beer-spoiling Lactobacillus brevis.
abstract: The tolerance to hop compounds, which is mainly associated with inhibition of bacterial growth in beer, is a multi-factorial trait. Any approaches to predict the physiological differences between beer-spoiling and non-spoiling strains on the basis of a single marker gene are limited. We identified ecotype-specific genes related to the ability to grow in Pilsner beer via comparative genome sequencing. The genome sequences of four different strains of Lactobacillus brevis were compared, including newly established genomes of two highly hop tolerant beer isolates, one strain isolated from faeces and one published genome of a silage isolate. Gene fragments exclusively occurring in beer-spoiling strains as well as sequences only occurring in non-spoiling strains were identified. Comparative genomic arrays were established and hybridized with a set of L. brevis strains, which are characterized by their ability to spoil beer. As result, a set of 33 and 4 oligonucleotide probes could be established specifically detecting beer-spoilers and non-spoilers, respectively. The detection of more than one of these marker sequences according to a genetic barcode enables scoring of L. brevis for their beer-spoiling potential and can thus assist in risk evaluation in brewing industry.
authors.affiliation: Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Str. 4, 85354 Freising, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Behr
authors.affiliation: Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Str. 4, 85354 Freising, Germany.
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Geissler
authors.affiliation: Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Str. 4, 85354 Freising, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Preissler
authors.affiliation: Technische Universität München, Lehrstuhl für Mikrobiologie, Emil-Ramann-Str 4, 85354 Freising, Germany.
authors.forename: Armin
authors.initials: A
authors.lastname: Ehrenreich
authors.affiliation: Technische Universität München, Lehrstuhl für Mikrobiologie, Emil-Ramann-Str 4, 85354 Freising, Germany.
authors.forename: Angel
authors.initials: A
authors.lastname: Angelov
authors.affiliation: Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Str. 4, 85354 Freising, Germany. Electronic address: rudi.vogel@wzw.tum.de.
authors.forename: Rudi F
authors.initials: RF
authors.lastname: Vogel
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001515:Beer / Q000382:microbiology*; D019295:Computational Biology; D003198:Computer Simulation; D060146:Ecotype; D005516:Food Microbiology; D005819:Genetic Markers; D016680:Genome, Bacterial; D052196:Levilactobacillus brevis / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D005819:Genetic Markers
keywords: Comparative genomics; Lactobacillus brevis