title: Construction of a Near-Infrared Fluorescent Turn-On Probe for Selenol and Its Bioimaging Application in Living Animals.
abstract: As selenocysteine (Sec) carries out the majority of the functions of the various Se-containing species in vivo, it is of high importance to develop reliable and rapid assays with biocompatibility to detect Sec. Herein, an NIR fluorescent turn-on probe for highly selective detection of selenol was designed and synthesized. The probe exhibits large turn-on signal upon treatment with selenocysteine (R-SeH), and it was further demonstrated that the new NIR fluorescent probe can be employed to image selenol in living animals.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082 (P. R. China).
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science of Jinan, Jinan, Shandong 250022 (P.R. China).
authors.forename: Baoli
authors.initials: B
authors.lastname: Dong
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science of Jinan, Jinan, Shandong 250022 (P.R. China).
authors.forename: Yonghe
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science of Jinan, Jinan, Shandong 250022 (P.R. China). weiyinglin2013@163.com.|State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082 (P. R. China). weiyinglin2013@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018036:Selenium Compounds / Q000737:chemistry*; D017279:Selenocysteine / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared
chemical_list: D005456:Fluorescent Dyes; D018036:Selenium Compounds; C442270:selenol; D017279:Selenocysteine
keywords: NIR-fluorescent fluorophores; fluorescence sensing; fluorescent probes; imaging agents; selenocysteine