title: miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment.
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Dingguo
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: You
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Lele
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China. Electronic address: bieping2010@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Bie
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D015854:Up-Regulation
chemical_list: C512511:ARID2 protein, human; C550906:MIRN208 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D014157:Transcription Factors; D053773:Transforming Growth Factor beta1
keywords: ARID2; Hepatocellular carcinoma; Invasion; Proliferation; miR-208-3p