title: Discrimination between two different grades of human glioma based on blood vessel infrared spectral imaging.
abstract: Gliomas are brain tumours classified into four grades with increasing malignancy from I to IV. The development and the progression of malignant glioma largely depend on the tumour vascularization. Due to their tissue heterogeneity, glioma cases can be difficult to classify into a specific grade using the gold standard of histological observation, hence the need to base classification on a quantitative and reliable analytical method for accurately grading the disease. Previous works focused specifically on vascularization study by Fourier transform infrared (FTIR) spectroscopy, proving this method to be a way forward to detect biochemical changes in the tumour tissue not detectable by visual techniques. In this project, we employed FTIR imaging using a focal plane array (FPA) detector and globar source to analyse large areas of glioma tumour tissue sections via molecular fingerprinting in view of helping to define markers of the tumour grade. Unsupervised multivariate analysis (hierarchical cluster analysis and principal component analysis) of blood vessel spectral data, retrieved from the FPA images, revealed the fine structure of the borderline between two areas identified by a pathologist as grades III and IV. Spectroscopic indicators are found capable of discriminating different areas in the tumour tissue and are proposed as biomolecular markers for potential future use of grading gliomas. Graphical Abstract Infrared imaging of glioma blood vessels provides a means to revise the pathologists' line of demarcation separating grade III (GIII) from grade IV (GIV) parts.
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK. Katia.Wehbe@Diamond.ac.uk.
authors.forename: Katia
authors.initials: K
authors.lastname: Wehbe
authors.affiliation: INSERM U1034, Université Bordeaux, 33600, Pessac, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Forfar
authors.affiliation: Department of Pathology, Pellegrin University Hospital, 33076, Bordeaux, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Eimer
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK.
authors.forename: Gianfelice
authors.initials: G
authors.lastname: Cinque
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply / Q000473:pathology*; D016000:Cluster Analysis; D005260:Female; D005910:Glioma / Q000098:blood supply / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D060787:Neoplasm Grading; D025341:Principal Component Analysis; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
keywords: Blood vessels; FPA imaging; FTIR spectroscopy; Glioma; Grading