title: Green tea catechins alone or in combination alter functional parameters of human neutrophils via suppressing the activation of TLR-4/NFκB p65 signal pathway.
abstract: The purpose of this study was to evaluate the potential of a mixture containing the four main catechins found in green tea, as well it separately, as modulators of the functional parameters of human neutrophils. The cells were obtained from peripheral blood of healthy individuals isolated and cultured with a mix: 30 μM of EGCG, 3 μM of EGC, 2 μM of ECG and 1.4 μM of EC, as well as each one alone. We evaluated the cytotoxicity of catechins, production of several reactive oxygen species (ROS), antioxidant enzymes (SOD, CAT, GPx and GR), Nrf2, TLR4/IKK/NFκB, CD11b mRNA levels, intracellular calcium release, chemotactic and phagocytic capacity, myeloperoxidase (MPO), and G6PDH activities, hypochlorous acid (HOCl) and pro-inflammatory cytokines release, protein levels of TLR4, p38 MAPK, iNOS and p-65 NFκB. The actions of the catechins were evidenced by the reduction in inflammatory parameters, including the suppression of TLR4, NFκB and iNOS protein expression, decreased release of TNF-α, IL-1β and IL-6, migration capacity, MPO activity and HOCl production and the suppression of ROS, nitric oxide and peroxynitrite production, while inducing antioxidant enzyme activities and Nrf2 mRNA levels, phagocytic capacity and calcium release. Our results demonstrate that catechins present marked immunomodulatory actions, either alone or in combination.
authors.affiliation: Postgraduate Program - Health Sciences - CBS, Universidade Cruzeiro do Sul, 03342000 São Paulo, SP, Brazil.
authors.forename: M P
authors.initials: MP
authors.lastname: Marinovic
authors.affiliation: Postgraduate Program - Health Sciences - CBS, Universidade Cruzeiro do Sul, 03342000 São Paulo, SP, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Morandi
authors.affiliation: Postgraduate Program - Health Sciences - CBS, Universidade Cruzeiro do Sul, 03342000 São Paulo, SP, Brazil. Electronic address: rosemari.otton@cruzeirodosul.edu.br.
authors.forename: R
authors.initials: R
authors.lastname: Otton
mesh_terms: D000328:Adult; D002374:Catalase / Q000378:metabolism; D002392:Catechin / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013662:Tea; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D017382:Reactive Oxygen Species; C495345:TLR4 protein, human; D013662:Tea; D051197:Toll-Like Receptor 4; D009569:Nitric Oxide; D006997:Hypochlorous Acid; D002392:Catechin; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Anti-inflammatory; Antioxidant; Flavonoids; Inflammation; Leukocyte; Polyphenols