title: CEMAsuite: open source degenerate PCR primer design.
abstract: The codon-equivalent multiple alignment suite begins conservational analysis for polymerase chain reaction primer design at the protein level, allowing the user to design consensus primers capable of detecting homologous coding sequences even when low-to-moderate sequence information is available. This package also condenses the wealth of information associated with multiple sequence alignments and presents them in an intuitive manner, allowing the user to quickly and effectively address degenerate primer design considerations.
  
  
  METHODS
  https://sourceforge.net/projects/cemasuite/.
  
  
  BACKGROUND
  benton@lsu.edu or cemasuite@gmail.com
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Courtney E
authors.initials: CE
authors.lastname: Lane
authors.affiliation: Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hulgan
authors.affiliation: Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Kelly
authors.initials: K
authors.lastname: O'Quinn
authors.affiliation: Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Benton
mesh_terms: D000465:Algorithms; D017931:DNA Primers / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D012984:Software; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers