title: Recognition and identification of active components from Radix Bupleuri using human neuroblastoma SH-SY5Y cells.
abstract: The aim of the study was to screen active components of Radix Bupleuri (a traditional Chinese herb) and discover novel anti-schizophrenic candidate drugs using human neuroblastoma SH-SY5Y cells. SH-SY5Y cells were used for preparation of the stationary phase in the cell membrane chromatography model. Retention components by the SH-SY5Y/CMC model were collected and then analyzed by GC/MS under the optimized conditions in offline conditions. After investigating the suitability and reliability of the SH-SY5Y/CMC method using amisulpride and haloperidol as standard compounds, this method was applied to screening active components from the extracts of Radix Bupleuri. Retention components of SH-SY5Y/CMC model were saikosaponin A, saikosaponin B1, saikosaponin B2, saikosaponin C and saikosaponin D, which were identified by the GC/MS method. In vitro pharmacological trials-MTT, saikosaponin B1, saikosaponin B2 and saikosaponin C could protect SY5Y cells. The protective effects of saikosaponin B1 and saikosaponin C were concentration dependent. Saikosaponin A and saikosaponin D inhibited cell viability at concentrations >30 µg/mL (p < 0.05). Via SH-SY5Y/CMC method and SH-SY5Y MTT trial, we rapidly detected target components from Radix Bupleuri, accurately identified them and determined their different effects on SH-SY5Y cells. Saikosaponin B1, saikosaponin B2 and saikosaponin C may be anti-schizophrenic candidate drugs.
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Liu
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Affiliated No. 1 hospital of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Tanghui
authors.initials: T
authors.lastname: Xu
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Quan
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Shi
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Zunxiao
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Xian Mental Health Center, Xian, Shaanxi, China.|The Affiliated Mental Health Center of Xi'an School of Medicine, Xian, Shaanxi, China.
authors.forename: Qiangju
authors.initials: Q
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D055808:Drug Discovery / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008516:Medicine, Chinese Traditional; D009447:Neuroblastoma; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D010946:Plants, Medicinal / Q000737:chemistry / Q000378:metabolism; D011619:Psychotropic Drugs; D015203:Reproducibility of Results; D012503:Saponins
chemical_list: D004365:Drugs, Chinese Herbal; D011619:Psychotropic Drugs; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D
keywords: active components; Radix Bupleuri; cell membrane chromatography (CMC); human neuroblastoma SH-SY5Y (SY5Y); saikosaponin