title: Bioavailability study of calcium sandoz-250 by atomic absorption spectroscopy in albino rats.
abstract: BACKGROUND
  Calcium sandoz-250 is an Ayurvedic calcium supplement, containing Khatika Churna. Bioavailability study of the formulation is essential for estimation of peak plasma concentration (C max), time to C max and rate of absorption.
  
  
  OBJECTIVE
  To evaluate the absorption parameters of calcium sandoz-250 in albino rats by atomic absorption spectroscopic (AAS) method.
  
  
  METHODS
  Study was carried out as a single dose, open-label, randomized study. Estimation of calcium was carried out by AAS, after validating the method for a few parameters for the estimation. Pharmacokinetic parameters such as C max, time to peak concentration (T max), area under the plasma concentration - time curve were calculated for calcium on administration of calcium sandoz-250.
  
  
  RESULTS
  Linearity curve was plotted for 0.5-2.5 ppm, given R (2) value 0.9975. The C max, i.e. C max after administration of calcium sandoz-250 was 0.793 mg/ml at 90 min (T max). Measurable calcium-blood levels were noticed in all subjects up to 3.0 h after administration of calcium sandoz-250.
  
  
  CONCLUSIONS
  Calcium sandoz-250, consisting of Khatika Churna, increases the blood calcium level in albino rats.
authors.affiliation: Department of Pharmaceutical Analysis, C.U. Shah College of Pharmacy and Research, Wadhwan, Gujarat, India.
authors.forename: Bimalkumar N
authors.initials: BN
authors.lastname: Patel
authors.affiliation: Department of Pharmaceutical Analysis, J.S.S. College of Pharmacy, Ooty, Tamil Nadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Krishnaveni
authors.affiliation: Department of Pharmaceutical Analysis, C.U. Shah College of Pharmacy and Research, Wadhwan, Gujarat, India.
authors.forename: Nurrrudin P
authors.initials: NP
authors.lastname: Jivani
authors.affiliation: Department of Pharmaceutics, C.U. Shah College of Pharmacy and Research, Wadhwan, Gujarat, India.
authors.forename: Akruti S
authors.initials: AS
authors.lastname: Khodakiya
authors.affiliation: Department of Pharmaceutics, C.U. Shah College of Pharmacy and Research, Wadhwan, Gujarat, India.
authors.forename: Moorti S
authors.initials: MS
authors.lastname: Khodakiya
authors.affiliation: Department of Pharmaceutical Analysis, C.U. Shah College of Pharmacy and Research, Wadhwan, Gujarat, India.
authors.forename: Saswat K
authors.initials: SK
authors.lastname: Parida
keywords: Ayurvedic formulation; bioavailability study; calcium sandoz-250