title: 14-3-3γ affects mTOR pathway and regulates lactogenesis in dairy cow mammary epithelial cells.
abstract: 14-3-3 proteins are an acidic protein family that is highly conserved and widely distributed in eukaryotic cells. Recent studies have found that 14-3-3 proteins play critical roles in cell signal transductions, cell growth and differentiation, and protein synthesis. 14-3-3γ is an important member of 14-3-3 protein family. In our previous study, we found that 14-3-3γ was upregulated by estrogen in dairy cow mammary epithelial cell (DCMEC), but the function and mechanism of 14-3-3γ is not known. In this experiment, we first cultured and purified the primary DCMEC and found 14-3-3γ located both in the cytoplasm and nucleus by using immunofluorescence assay. Methionine, lysine, estrogen, and prolactin could upregulate the expression of 14-3-3γ, stimulate the secretion of β-casein and triglyceride, and raise the cell viability of DCMEC. We constructed a stable 14-3-3γ overexpression cell line of DCMEC and found that the expressions of mTOR and p-mTOR, the secretion of triglyceride and β-casein (CSN2), and the cell viability of DCMEC were all upregulated. We also observed the effects of 14-3-3γ gene silencing and gained consistent results with 14-3-3γ overexpression. These findings reveal that 14-3-3γ affects the mTOR pathway and regulates lactogenesis in DCMECs.
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Nagam
authors.initials: N
authors.lastname: Khudhair
authors.forename: Chaochao
authors.initials: C
authors.lastname: Luo
authors.forename: Ahmed
authors.initials: A
authors.lastname: Khalid
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Jinxia
authors.initials: J
authors.lastname: Ao
authors.forename: Qingzhang
authors.initials: Q
authors.lastname: Li
authors.forename: Xuejun
authors.initials: X
authors.lastname: Gao
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D003612:Dairying; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007774:Lactation; D008239:Lysine / Q000378:metabolism / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D002364:Caseins; D014280:Triglycerides; D011388:Prolactin; D008715:Methionine; D058570:TOR Serine-Threonine Kinases; D008239:Lysine