title: Targeted fluorescent probes for detection of oxidative stress in the mitochondria.
abstract: Mitochondrial oxidative stress has been implicated in aging, neurodegenerative diseases, diabetes, stroke, ischemia/reperfusion injury, age-related macular degeneration (AMD) and cancer. Recently, we developed two new mitochondria-targeting fluorescent probes, MitoProbes I/II, which specifically localize in mitochondria and employed both in vivo and in vitro for detection of mitochondrial oxidative stress. Here, we report the design and synthesis of these agents, as well as their utility for real-time imaging of mitochondrial oxidative stress in cells.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI, USA.
authors.forename: Nazmiye B
authors.initials: NB
authors.lastname: Yapici
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI, USA.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mandalapu
authors.affiliation: Experimental & Systems Pharmacology, College of Pharmacy, Washington State University, Spokane, WA 99201, USA.
authors.forename: K Michael
authors.initials: KM
authors.lastname: Gibson
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI, USA. Electronic address: Lanrong@mtu.edu.
authors.forename: Lanrong
authors.initials: L
authors.lastname: Bi
mesh_terms: D005456:Fluorescent Dyes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D008958:Models, Molecular; D018384:Oxidative Stress
chemical_list: D005456:Fluorescent Dyes
keywords: Fluorescence probes; Mitochondria-targeting; Oxidative stress