title: Three-dimensional co-culture of BM-MSCs and eccrine sweat gland cells in Matrigel promotes transdifferentiation of BM-MSCs.
abstract: Victims with extensive and deep burns are unable to regenerate eccrine sweat glands. Combining of stem cells and biomimetic ECM to generate cell-based 3D tissues is showing promise for tissue repair and regeneration. We co-cultured BrdU-labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) and eccrine sweat gland cells in Matrigel for 2 weeks in vitro and then evaluated for BM-MSCs differentiation into functional eccrine sweat gland cells by morphological assessment and immunohistochemical double staining for BrdU/pancytokeratin, BrdU/ZO-2, BrdU/E-cadherin, BrdU/desmoglein-2, BrdU/Na(+)-K(+)-ATPase α, BrdU/NHE1 and BrdU/CFTR. Cells formed spheroid-like structures in Matrigel, and BrdU-labeled BM-MSCs were involved in the 3D reconstitution of eccrine sweat gland tissues, and the incorporated BM-MSCs expressed an epithelial cell marker (pancytokeratin), epithelial cell junction proteins (ZO-2, E-cadherin and desmoglein-2) and functional proteins of eccrine sweat glands (Na(+)-K(+)-ATPase α, NHE1 and CFTR). In conclusion, three-dimensional co-culture of BM-MSCs and eccrine sweat gland cells in Matrigel promotes the transdifferentiation of BM-MSCs into potentially functional eccrine sweat gland cells.
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China, lihaihong1051@126.com.
authors.forename: Haihong
authors.initials: H
authors.lastname: Li
authors.forename: Xuexue
authors.initials: X
authors.lastname: Li
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Bingna
authors.initials: B
authors.lastname: Zhang
authors.forename: Shijie
authors.initials: S
authors.lastname: Tang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D000293:Adolescent; D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D054338:Cell Transdifferentiation; D002648:Child; D018920:Coculture Techniques; D003094:Collagen; D004338:Drug Combinations; D004439:Eccrine Glands / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007797:Laminin; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D011509:Proteoglycans; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen