title: Photoexcited Porphyrins as a Strong Suppressor of β-Amyloid Aggregation and Synaptic Toxicity.
abstract: The abnormal assembly of β-amyloid (Aβ) peptides into neurotoxic, β-sheet-rich amyloid aggregates is a major pathological hallmark of Alzheimer's disease (AD). Light-induced photosensitizing molecules can regulate Aβ amyloidogenesis. Multiple photochemical analyses using circular dichroism, atomic force microscopy, dot blot, and native gel electrophoresis verified that photoactivated meso-tetra(4-sulfonatophenyl)porphyrin (TPPS with M = 2H(+), Zn(2+), Cu(2+), Mn(2+)) successfully inhibits Aβ aggregation in vitro. Furthermore, Aβ toxicity was relieved in the photoexcited-TPPS-treated Drosophila AD model. TPPS suppresses neural cell death, synaptic toxicity, and behavioral defects in the Drosophila AD model under blue light illumination. Behavioral phenotypes, including larval locomotion defect and short lifespan caused by Aβ overexpression, were also rescued by blue light-excited TPPS.
authors.affiliation: Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 335 Science Road, Daejeon 305-701 (Republic of Korea).
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, Korea University of Science and Technology (UST), Daejeon 305-333 (Korea).|Gwangju Center, Korea Basic Science Institute (KBSI), Gwangju 500-757 (Korea).
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Lee
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, Korea University of Science and Technology (UST), Daejeon 305-333 (Korea).
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Suh
authors.affiliation: Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 335 Science Road, Daejeon 305-701 (Republic of Korea).
authors.forename: Joon Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, Korea University of Science and Technology (UST), Daejeon 305-333 (Korea).
authors.forename: Ae-kyeong
authors.initials: AK
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College of Medicine, Chungnam National University, Daejeon 301-747 (Korea).
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
authors.affiliation: Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Department of Functional Genomics, Korea University of Science and Technology (UST), Daejeon 305-333 (Korea). kweonyu@kribb.re.kr.
authors.forename: Kweon
authors.initials: K
authors.lastname: Yu
authors.affiliation: Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 335 Science Road, Daejeon 305-701 (Republic of Korea). parkcb@kaist.ac.kr.
authors.forename: Chan Beum
authors.initials: CB
authors.lastname: Park
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D004330:Drosophila; D055668:Photochemical Processes; D011166:Porphyrins / Q000737:chemistry*; D013569:Synapses / Q000187:drug effects*
chemical_list: D016229:Amyloid beta-Peptides; D011166:Porphyrins
keywords: Alzheimer’s disease; Drosophila model; photosensitizers; porphyrins; β-amyloids