title: Effects of daidzein on alveolar bone loss and internal microstructures of bone in a rat model of experimental periodontitis: a study using micro-computed tomography.
abstract: OBJECTIVE
  Daidzein is an isoflavone abundant in soybeans, kudzu root and red clover, which have been widely studied for its therapeutic potential. The present study was designed to evaluate the effects of daidzein on alveolar bone loss and internal microstructures of bone in a rat model of experimental periodontitis by assessing morphological data obtained from micro-computed tomography (micro-CT).
  
  
  METHODS
  Twenty-four male Sprague-Dawley rats were randomly assigned to the following three groups comprising eight animals each: the nonligation (NL) group; the ligation (L) group; and the ligation+daidzein (LD) group. To induce periodontitis, a 4-0 braided silk ligature was tied around the cervical area of the lower-right first molars of rats in groups L and LD. Rats in the LD group were given daily doses of daidzein (10 mg/kg of body weight) by intraperitoneal injection immediately after ligature placement. Two weeks after the placement of ligatures, mandibular block biopsies were scanned using a micro-CT system.
  
  
  RESULTS
  Daily administration of daidzein strongly suppressed the ligature-induced loss of alveolar bone height. In addition, when rats were treated with daidzein, the ligature-induced decrease in the bone volume fraction was significantly recovered. Furthermore, daidzein significantly reversed ligature-induced deteriorations in the microarchitecture parameters of trabecular bone, such as trabecular thickness, bone mineral density, trabecular separation and structure model index.
  
  
  CONCLUSIONS
  The study presented here demonstrates, for the first time, that daidzein effectively reduces alveolar bone destruction resulting from experimental periodontitis in rats. Further studies are necessary for the translation of this compound clinically to improve the outcomes of patients diagnosed with periodontitis.
authors.affiliation: Department of Periodontology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam-do, Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Bae
authors.affiliation: Department of Periodontology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam-do, Korea.
authors.forename: M H
authors.initials: MH
authors.lastname: Ha
authors.affiliation: Department of Biological Science, College of Medical and Life Sciences, Silla University, Busan, Korea.
authors.forename: E-Y
authors.initials: EY
authors.lastname: Choi
authors.affiliation: Department of Periodontology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam-do, Korea.|Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Gyeongsangnam-do, Korea.
authors.forename: J-I
authors.initials: JI
authors.lastname: Choi
authors.affiliation: Department of Biological Science, College of Medical and Life Sciences, Silla University, Busan, Korea.
authors.forename: I S
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Department of Periodontology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam-do, Korea.|Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Gyeongsangnam-do, Korea.|Institute of Translational Dental Sciences, Pusan National University, Yangsan, Gyeongsangnam-do, Korea.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D016301:Alveolar Bone Loss / Q000139:chemically induced; D000818:Animals; D015519:Bone Density; D008297:Male; D010518:Periodontitis; D051381:Rats; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
keywords: alveolar bone; daidzein; experimental periodontitis; micro-computed tomography