title: Nosema bombycis (Microsporidia) suppresses apoptosis in BmN cells (Bombyx mori).
abstract: Nosema bombycis (N. bombycis, Nb) is a fungus-related and obligate intracellular parasite that causes chronic pebrine disease in the silkworm. After infecting the host, spores obtain energy from host cells and survive for several days. This symbiosis between the pathogen and the host cell suggests that N. bombycis prevents apoptosis and reactive oxygen species (ROS) production of host cells to create the optimal environmental conditions for its growth and development. In this study, different methods were used to prove that N. bombycis suppressed apoptosis in BmN cells. Flow cytometry analysis results showed that spores suppressed apoptosis of BmN cells at 2 and 5 days after infection (P < 0.05). Compared with actinomycin D (ActD) treatment, apoptosis of BmN cells was apparently reduced after spore infection (P < 0.01). Forty-eight hours after infection, the ROS production of BmN cells was down-regulated compared with that after ActD treatment for 6 h. Furthermore, N. bombycis prevented the formation of apoptosomes by down-regulating the expression of apaf-1 and cytochrome C. In addition, N. bombycis also up-regulated the expression of buffy. Western blot analysis demonstrated that spores decreased the level of host cytochrome C at 48 and 98 h post infection. Thus, our results suggested that N. bombycis inhibited the mitochondrial apoptotic pathway of the host cells to create an optimal environment for its own survival.
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: He
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhangwuke
authors.initials: Z
authors.lastname: Fu
authors.affiliation: TongDe Hospital of Zhejiang Province, Hangzhou 310058, China.
authors.forename: Mingqian
authors.initials: M
authors.lastname: Li
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shunfeng
authors.initials: S
authors.lastname: Cai
authors.affiliation: Hangzhou Seed Station, Hangzhou 310029, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Man
authors.affiliation: Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China xmlu@zju.edu.cn.
authors.forename: Xingmeng
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D012831:Bombyx; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005434:Flow Cytometry; D016818:Nosema / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D003609:Dactinomycin; D045304:Cytochromes c
keywords: Nosema bombycis; ROS; apoptosis; cytochrome C; inhibition