title: Sixteen polymorphic microsatellite markers for a federally threatened species, Hexastylis naniflora (Aristolochiaceae), and co-occurring congeners.
abstract: OBJECTIVE
  Twenty microsatellite loci were developed for the federally threatened species Hexastylis naniflora (Aristolochiaceae) to examine genetic diversity and to distinguish this species from co-occurring congeners, H. heterophylla and H. minor.
  
  
  RESULTS
  Next-generation sequencing approaches were used to identify microsatellite loci and design primers. One hundred fifty-two primer pairs were screened for repeatability, and 20 of these were further characterized for polymorphism. In H. naniflora, the number of alleles identified for polymorphic loci ranged from two to 23 (mean ∼8.8), with a mean heterozygosity of 0.39.
  
  
  CONCLUSIONS
  These 16 polymorphic primers for H. naniflora will be useful tools in species identification and quantifying genetic diversity within the genus.
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Jacqueline W
authors.initials: JW
authors.lastname: Hamstead
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Brandon L
authors.initials: BL
authors.lastname: Snider
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Robyn
authors.initials: R
authors.lastname: Oaks
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Evan
authors.initials: E
authors.lastname: Fitzgerald
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Woodward
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Teat
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Nikolai M
authors.initials: NM
authors.lastname: Hay
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Matt C
authors.initials: MC
authors.lastname: Estep
authors.affiliation: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28608 USA.
authors.forename: Zack E
authors.initials: ZE
authors.lastname: Murrell
keywords: Aristolochiaceae; Asarum; Hexastylis; Hexastylis naniflora; hybrid; microsatellite markers