title: An "All-laser" Endothelial Transplant.
abstract: The "all laser" assisted endothelial keratoplasty is a procedure that is performed with a femtosecond laser used to cut the donor tissue at an intended depth, and a near infrared diode laser to weld the corneal tissue. The proposed technique enables to reach the three main goals in endothelial keratoplasty: a precise control in the thickness of the donor tissue; its easy insertion in the recipient bed and a reduced risk of donor lenticule dislocation. The donor cornea thickness is measured in the surgery room with optical coherence tomography (OCT), in order to correctly design the donor tissue dimensions. A femtosecond laser is used to cut the donor cornea. The recipient eye is prepared by manual stripping of the descemetic membrane. The donor endothelium is inserted into a Busin-injector, the peripheral inner side is stained with a proper chromophore (a water solution of Indocyanine Green) and then it is pulled in the anterior chamber. The transplanted tissue is placed in the final and correct location and then diode laser welding is induced from outside the eyeball. The procedure has been performed on more than 15 patients evidencing an improvement in surgery performances, with a good recovery of visual acuity and a reduced donor lenticule dislocation event.
authors.affiliation: Institute of Applied Physics, Italian National Research Council; f.rossi@ifac.cnr.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rossi
authors.affiliation: U.O. Oculistica, Nuovo Ospedale Santo Stefano.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Canovetti
authors.affiliation: U.O. Oculistica, Nuovo Ospedale Santo Stefano.
authors.forename: Alex
authors.initials: A
authors.lastname: Malandrini
authors.affiliation: U.O. Oculistica, Nuovo Ospedale Santo Stefano.
authors.forename: Ivo
authors.initials: I
authors.lastname: Lenzetti
authors.affiliation: Institute of Applied Physics, Italian National Research Council.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pini
authors.affiliation: U.O. Oculistica, Nuovo Ospedale Santo Stefano.
authors.forename: Luca
authors.initials: L
authors.lastname: Menabuoni
mesh_terms: D016039:Corneal Transplantation / Q000379:methods*; D004728:Endothelium, Corneal / Q000637:transplantation*; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D014019:Tissue Donors; D041623:Tomography, Optical Coherence / Q000379:methods; D014792:Visual Acuity