title: Diagnosis of the strongyloid nematode Strongyloides venezuelensis in experimentally infected rats.
abstract: Strongyloides venezuelensis is an intestinal nematode of rats, frequently used as a model for studying human and animal strongyloidiasis. In the present study, we evaluated parasitological, serological and molecular methods for the diagnosis of experimental S. venezuelensis in rats, Rattus norvegicus. Blood and faecal samples were collected and analysed up to 60 days post infection (pi) with adult worm recovery occurring from 5 to 45 days pi. Using an enzyme-linked immunosorbent assay (ELISA), serum levels of IgG antibodies increased up to 28 days pi, thereafter decreasing by day 60 pi. Polymerase chain reaction (PCR) assays detected S. venezuelensis DNA in faecal samples of rats from 5 to 21 days pi. The present study therefore represents the first step towards improving the diagnosis of experimental strongyloidiasis.
authors.affiliation: Instituto de Medicina Tropical de São Paulo.
authors.forename: P D
authors.initials: PD
authors.lastname: Marques
authors.affiliation: Laboratório de Gastroenterologia e Hepatologia Tropical, Hospital das Clínicas da Faculdade de Medicina.
authors.forename: F M
authors.initials: FM
authors.lastname: Malta
authors.affiliation: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo,São Paulo,Brazil.
authors.forename: D M C L
authors.initials: DM
authors.lastname: Meisel
authors.affiliation: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo,São Paulo,Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Corral
authors.affiliation: Laboratório de Gastroenterologia e Hepatologia Tropical, Hospital das Clínicas da Faculdade de Medicina.
authors.forename: J R
authors.initials: JR
authors.lastname: Pinho
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Universidade Federal de Uberlândia, Uberlândia,Minas Gerais,Brazil.
authors.forename: J M
authors.initials: JM
authors.lastname: Costa-Cruz
authors.affiliation: Faculdade de Ciências Médicas, Santa Casa,São Paulo,Brazil.
authors.forename: P P
authors.initials: PP
authors.lastname: Chieffi
authors.affiliation: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo,São Paulo,Brazil.
authors.forename: R C B
authors.initials: RC
authors.lastname: Gryschek
authors.affiliation: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo,São Paulo,Brazil.
authors.forename: F M
authors.initials: FM
authors.lastname: Paula
mesh_terms: D000818:Animals; D001769:Blood / Q000469:parasitology; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D025202:Molecular Diagnostic Techniques / Q000379:methods; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction; D051381:Rats; D012698:Serologic Tests / Q000379:methods; D013321:Strongyloides / Q000302:isolation & purification*; D013322:Strongyloidiasis / Q000175:diagnosis*