title: Deletion of the budBAC operon in Klebsiella pneumoniae to understand the physiological role of 2,3-butanediol biosynthesis.
abstract: Klebsiella pneumoniae is known to produce 2,3-butanediol (2,3-BDO), a valuable chemical. In K. pneumoniae, the 2,3-BDO operon (budBAC) is involved in the production of 2,3-BDO. To observe the physiological role of the 2,3-BDO operon in a mixed acid fermentation, we constructed a budBAC-deleted strain (SGSB109). The production of extracellular metabolites, CO2 emission, carbon distribution, and NADH/NAD(+) balance of SGSB109 were compared with the parent strain (SGSB100). When comparing the carbon distribution at 15 hr, four significant differences were observed: in 2,3-BDO biosynthesis, lactate and acetate production (lactate and acetate production increased 2.3-fold and 4.1-fold in SGSB109 compared to SGSB100), CO2 emission (higher in SGSB100), and carbon substrate uptake (higher in SGSB100). Previous studies on the inactivation of the 2,3-BDO operon were focused on the increase of 1,3-propanediol production. Few studies have been done observing the role of 2,3-BDO biosynthesis. This study provides a prime insight into the role of 2,3-BDO biosynthesis of K. pneumoniae.
authors.affiliation: a Department of Chemical and Biomolecular Engineering , Sogang University , Seoul , South Korea.
authors.forename: Daun
authors.initials: D
authors.lastname: Jeong
authors.affiliation: a Department of Chemical and Biomolecular Engineering , Sogang University , Seoul , South Korea.
authors.forename: Jeongmo
authors.initials: J
authors.lastname: Yang
authors.affiliation: a Department of Chemical and Biomolecular Engineering , Sogang University , Seoul , South Korea.
authors.forename: Soojin
authors.initials: S
authors.lastname: Lee
authors.affiliation: a Department of Chemical and Biomolecular Engineering , Sogang University , Seoul , South Korea.
authors.forename: Borim
authors.initials: B
authors.lastname: Kim
authors.affiliation: b Korea Clean Energy Center , Korea Institute of Science and Technology , Seoul , South Korea.
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um
authors.affiliation: c Institute of Life Sciences and Resources and Department of Food Science and Biotechnology , Kyung Hee University , Yongin , South Korea.
authors.forename: Youngrok
authors.initials: Y
authors.lastname: Kim
authors.affiliation: a Department of Chemical and Biomolecular Engineering , Sogang University , Seoul , South Korea.
authors.forename: Kyoung-Su
authors.initials: KS
authors.lastname: Ha
authors.affiliation: a Department of Chemical and Biomolecular Engineering , Sogang University , Seoul , South Korea.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D002072:Butylene Glycols / Q000378:metabolism*; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009876:Operon
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol
keywords: 2,3-butanediol; CO2 emission; budBAC operon; carbon distribution analysis; redox balance