title: Flavonoids and Other Phenolic Compounds in Needles of Pinus peuce and Other Pine Species from the Macedonian Flora.
abstract: Flavonoids and other phenolic compounds in young needles of four pine species, Pinus peuce, P. nigra, P. mugo and P. sylvestris from the Macedonian flora were investigated. The amount of total phenols and total flavonoids were determined using Folin-Ciocalteau and aluminum chloride assay, respectively. The obtained results revealed that the total phenolic content (TPC) and total flavonoids content (TFC) varied among different pine species ranging from 9.8 to 14.0 mg GAE/g and from 3.3 to 7.2 mg CE/g of dried plant material, respectively. Qualitative analysis of flavonoids and other phenolic components was made by a LC-DAD/ESI-MS(n) optimized chromatographic method. A total of 17 phenolic components were identified and classified as: acids (2), procyanidins (2) and flavonoid glycosides (13). The most prevalent components were flavonoid glycosides, especially flavonols and methylated flavonols (9). Additionally, 3 components were found as acylated flavonol glycosides with ferulic and p-coumaric acid. The last one was found not only in esterified form but also in the free form. Only one flavone-apigenin glycoside was detected. Procyanidins were identified as catechin derivatives, both dimers and trimers.
authors.forename: Marija
authors.initials: M
authors.lastname: Karapandzova
authors.forename: Gjose
authors.initials: G
authors.lastname: Stefkov
authors.forename: Ivana
authors.initials: I
authors.lastname: Cvetkovikj
authors.forename: Jasmina Petreska
authors.initials: JP
authors.lastname: Stanoeva
authors.forename: Marina
authors.initials: M
authors.lastname: Stefova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Kulevanova
mesh_terms: D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D028223:Pinus / Q000737:chemistry* / Q000145:classification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018843:Republic of North Macedonia
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts