title: Stanniocalcin 1 effects on the renal gluconeogenesis pathway in rat and fish.
abstract: The mammalian kidney contributes significantly to glucose homeostasis through gluconeogenesis. Considering that stanniocalcin 1 (STC1) regulates ATP production, is synthesized and acts in different cell types of the nephron, the present study hypothesized that STC1 may be implicated in the regulation of gluconeogenesis in the vertebrate kidney. Human STC1 strongly reduced gluconeogenesis from (14)C-glutamine in rat renal medulla (MD) slices but not in renal cortex (CX), nor from (14)C-lactic acid. Total PEPCK activity was markedly reduced by hSTC1 in MD but not in CX. Pck2 (mitochondrial PEPCK isoform) was down-regulated by hSTC1 in MD but not in CX. In fish (Dicentrarchus labrax) kidney slices, both STC1-A and -B isoforms decreased gluconeogenesis from (14)C-acid lactic, while STC1-A increased gluconeogenesis from (14)C-glutamine. Overall, our results demonstrate a role for STC1 in the control of glucose synthesis via renal gluconeogenesis in mammals and suggest that it may have a similar role in teleost fishes.
authors.affiliation: Pos-Graduate Program in Biological Sciences, Department of Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil; Pos-Graduate Program in Health Sciences, Department of Obstetrics and Gynecology, School of Medicine, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2400, 90035-003, Porto Alegre, RS, Brazil; CCMAR - Centre for Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal. Electronic address: 00107953@ufrgs.br.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Schein
authors.affiliation: Pos-Graduate Program in Biological Sciences, Department of Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Luiz C
authors.initials: LC
authors.lastname: Kucharski
authors.affiliation: CCMAR - Centre for Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Pedro M G
authors.initials: PM
authors.lastname: Guerreiro
authors.affiliation: Pos-Graduate Program in Biological Sciences, Department of Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Tiago Leal
authors.initials: TL
authors.lastname: Martins
authors.affiliation: CCMAR - Centre for Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Morgado
authors.affiliation: CCMAR - Centre for Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Power
authors.affiliation: CCMAR - Centre for Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Adelino V M
authors.initials: AV
authors.lastname: Canario
authors.affiliation: Pos-Graduate Program in Biological Sciences, Department of Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Roselis S M
authors.initials: RS
authors.lastname: da Silva
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D005943:Gluconeogenesis; D005973:Glutamine / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008322:Mammals / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D029941:Fish Proteins; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D005973:Glutamine; D019344:Lactic Acid; C051795:teleocalcin; C582554:Pck1 protein, rat; D010729:Phosphoenolpyruvate Carboxykinase (GTP)
keywords: Fish; Gluconeogenesis; Kidney; PEPCK; Rat; Stanniocalcin