title: Derlin-1 is overexpressed in human colon cancer and promotes cancer cell proliferation.
abstract: Derlin-1 is overexpressed in many types of solid tumors and plays an important role in cancer progression. However, the expression pattern and functions of Derlin-1 in human colon cancer are not fully understood. In the present study, we examined Derlin-1 expression in colon cancer cell lines and human tissues and investigated its role in colon cancer. We found that Derlin-1 expression was increased significantly in colon cancer tissues and its overexpression correlated with the tumor differentiation, Dukes stage, invasion, lymph node metastasis, distant metastasis, and poor overall survival. The silencing of Derlin-1 by shRNA led to the growth inhibition of colon cancer cells, which were associated with the promotion of apoptosis. Furthermore, Derlin-1 silencing significantly inhibited the activation of the PI3K/AKT signaling pathway. Taken together, our results showed that Derlin-1 is overexpressed in colon cancer and promotes proliferation of colon cancer cells. Derlin-1 may be a potential therapeutic target for the treatment of colon cancer.
authors.affiliation: Department of Gastroenterology, Nanjing Dachang Hospital, 68 Taizishan Rd, Yanjiang Industrial Development Zone, Nanjing, 210044, Jiangsu, China. xumingtan@126.com.
authors.forename: Xueming
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Apheresis, Nanjing Red Cross Blood Center, 3 Zizhulin, Nanjing, 210003, Jiangsu, China. 1252518909@qq.com.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan, Nanjing, 210011, Jiangsu, China. jiangzhonghua1982@163.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan, Nanjing, 210011, Jiangsu, China. xhongwang@126.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan, Nanjing, 210011, Jiangsu, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Endoscopy Center, The First Affiliated of Hospital of Nanjing Medical University, 300 Guangzhou St., Nanjing, 210029, Jiangsu, China. liuli306@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Endoscopy Center, The First Affiliated of Hospital of Nanjing Medical University, 300 Guangzhou St., Nanjing, 210029, Jiangsu, China. wxnanjing@163.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endoscopy Center, The First Affiliated of Hospital of Nanjing Medical University, 300 Guangzhou St., Nanjing, 210029, Jiangsu, China. fanzhining@njmu.edu.cn.
authors.forename: Zhining
authors.initials: Z
authors.lastname: Fan
authors.affiliation: The Metabolic Disease Research Centre, Nanjing Medical University, 140 Hanzhong St., Nanjing, 210029, Jiangsu, China. su_dongming@hotmail.com.
authors.forename: Dongming
authors.initials: D
authors.lastname: Su
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D016019:Survival Analysis; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: C487709:DERL1 protein, human; D008565:Membrane Proteins
keywords: Apoptosis; Derlin-1; Human colon cancer; Proliferation