title: The interactions of squalene, alkanes and other mineral oils with model membranes; effects on membrane heterogeneity and function.
abstract: Droplet interface bilayers (DIBs) offer many favourable facets as an artificial membrane system but the influence of any residual oil that remains in the bilayer following preparation is ill-defined. In this study the fluorescent membrane probes di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (Di-8-ANEPPS) and Fluoresceinphosphatidylethanolamine (FPE) were used to help understand the nature of the phospholipid-oil interaction and to examine any structural and functional consequences of such interactions on membrane bilayer properties. Concentration-dependent modifications of the membrane dipole potential were found to occur in phospholipid vesicles exposed to a variety of different oils. Incorporation of oil into the lipid bilayer was shown to have no significant effect on the movement of fatty acids across the lipid bilayer. Changes in membrane heterogeneity were, however, demonstrated with increased microdomain formation being visible in the bilayer following exposure to mineral oil, pentadecane and squalene. As it is important that artificial systems provide an accurate representation of the membrane environment, careful consideration should be taken prior to the application of DIBs in studies of membrane structure and organisation.
authors.affiliation: Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science, School of Life Sciences, University of Nottingham, University Park, Nottingham, United Kingdom. Electronic address: joanna.richens@nottingham.ac.uk.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Richens
authors.affiliation: Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science, School of Life Sciences, University of Nottingham, University Park, Nottingham, United Kingdom. Electronic address: jordan.lane@nottingham.ac.uk.
authors.forename: Jordan S
authors.initials: JS
authors.lastname: Lane
authors.affiliation: Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science, School of Life Sciences, University of Nottingham, University Park, Nottingham, United Kingdom. Electronic address: melissa.mather@nottingham.ac.uk.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Mather
authors.affiliation: Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science, School of Life Sciences, University of Nottingham, University Park, Nottingham, United Kingdom. Electronic address: paul.oshea@nottingham.ac.uk.
authors.forename: Paul
authors.initials: P
authors.lastname: O'Shea
mesh_terms: D000473:Alkanes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D008899:Mineral Oil / Q000737:chemistry*; D008958:Models, Molecular; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D013185:Squalene / Q000737:chemistry*
chemical_list: D000473:Alkanes; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010743:Phospholipids; D013185:Squalene; D008899:Mineral Oil
keywords: Alkanes; Droplet interface bilayers; Membrane microdomains; Mineral oil; Squalene