title: Superovulation Induced Changes of Lipid Metabolism in Ovaries and Embryos and Its Probable Mechanism.
abstract: This research was intended to investigate the fetal origins of changed birth weight of the offspring born through assisted reproductive technology (ART). The association between hormone and lipid metabolism or body weight has been generally accepted, and as the basic and specific treatment in ART procedure, gonadotropin stimulation might have potential effects on intrauterine lipid metabolism. In our studies, the mice were superovulated with two doses of gonadotropin. The cholesterol metabolism in ovaries and the triglyceride metabolism in embryos were analyzed. The results showed gonadotropin probably accelerated luteinization and induced a longer time follicle development and ovulation, which resulted in histological and morphological alteration of ovary, and increased the cholesterol content and the expressions of steroidogenesis-related genes. In embryos, gonadotropin increased lipid accumulation and decreased fatty acid synthesis in a dose-dependent manner. Moreover, the changes of fatty acid composition were also shown in superovulation groups. Our studies firstly provided the evidence that the superovulation might affect the maternal and fetal lipid metabolism. These variations of lipid metabolism in our results may be associated with birth weight of ART infants.
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Li-Ya
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Le
authors.affiliation: Department of Gynecologic Oncology, Henan Cancer Hospital, Zhengzhou, 450003, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Hang-Ying
authors.initials: HY
authors.lastname: Lou
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Liu
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Ying-Ming
authors.initials: YM
authors.lastname: Zheng
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Ye-Qing
authors.initials: YQ
authors.lastname: Qian
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Chen
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Xin-Hang
authors.initials: XH
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China; International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, 910 Hengshan Road, Shanghai, 200030, China.
authors.forename: He-Feng
authors.initials: HF
authors.lastname: Huang
authors.affiliation: Centre of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Health, Hangzhou, 310006, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jin
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006062:Gonadotropins / Q000502:physiology; D050356:Lipid Metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010053:Ovary / Q000378:metabolism*; D011374:Progesterone / Q000097:blood; D013480:Superovulation
chemical_list: D006062:Gonadotropins; D011374:Progesterone