title: MicroRNA-365 inhibits growth, invasion and metastasis of malignant melanoma by targeting NRP1 expression.
abstract: OBJECTIVE
  The role of miR-365 in cancer cells seemed controversial in previous studies. We thereby in this article aimed to define the role of miR-365 in malignant melanoma (MM) pathogenesis.
  
  
  METHODS
  We detected miR-365 expression in malignant melanoma cell lines and then investigated the effects of miR-365 on the metastasis and malignancy of melanoma cells. The correlation between miR-365 level and NRP1 (neuropilin1) was further investigated in clinical malignant melanoma specimens.
  
  
  RESULTS
  MiR-365 was strongly down-regulated in malignant melanoma (MM) tissues and cell lines, and its expression levels were associated with lymph node metastasis and clinical stage, as well as overall survival and replase-free survival of MM. We also found that ectopic expression of miR-365 inhibited MM cell proliferation and MM metastasis in vitro and in vivo. We further identified a novel mechanism of miR-365 to suppress MM growth and metastasis. NRP1 was proved to be a direct target of miR-365, using luciferase assay and western blot. NRP1 over-expression in miR-365 expressing cells could rescue invasion and growth defects of miR-365. In addition, miR-365 expression inversely correlated with NRP1 protein levels in MM.
  
  
  CONCLUSIONS
  Our data suggest that miR-365 functions as a tumor suppressor in MM development and progression, and holds promise as a prognostic biomarker and potential therapeutic target for MM.
authors.affiliation: Plastic Surgery, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Bai
authors.affiliation: Plastic Surgery, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou, China.
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Plastic Surgery, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: Plastic Surgery, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou, China.
authors.forename: Huifan
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000556:secondary / Q000628:therapy; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D039942:Neuropilin-1 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012307:Risk Factors; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: C546819:MIRN365 microRNA, human; D035683:MicroRNAs; D039942:Neuropilin-1
keywords: Malignant melanoma (MM); cancer biomarker; invasion and metastasis; miR-365; neuropilin1 (NRP1); pathogenesis; target therapy