title: Yindanxinnaotong, a Chinese compound medicine, synergistically attenuates atherosclerosis progress.
abstract: Yindanxinnaotong (YD), a traditional Chinese medicine, has been introduced to clinical medicine for more than a decade, while its pharmacological properties are still not to be well addressed. This report aimed to explore the anti-atherosclerosis properties and underlying mechanisms of YD. We initially performed a computational prediction based on a network pharmacology simulation, which clued YD exerted synergistically anti-atherosclerosis properties by vascular endothelium protection, lipid-lowering, anti-inflammation, and anti-oxidation. These outcomes were then validated in atherosclerosis rats. The experiments provided evidences indicating YD's contribution in this study included, (1) significantly reduced the severity of atherosclerosis, inhibited reconstruction of the artery wall and regulated the lipid profile; (2) enhanced antioxidant power, strengthened the activity of antioxidant enzymes, and decreased malondialdhyde levels; (3) significantly increased the viability of umbilical vein endothelial cells exposed to oxidative stress due to pretreatment with YD; (4) significantly reduced the level of pro-inflammatory cytokines; (5) significantly down-regulated NF-kB/p65 and up-regulated IkB in the YD-treated groups. Overall, these results demonstrated that YD intervention relieves atherosclerosis through regulating lipids, reducing lipid particle deposition in the endothelial layer of artery, enhancing antioxidant power, and repressing inflammation activity by inhibiting the nuclear factor-kappa B signal pathway.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, P. R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Traditional Chinese Medicine, Beijing Shijitan Hospital affiliated with Capital Medical University, Beijing, 100038, P. R. China.
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Pan
authors.affiliation: Center for Drug Evaluation, China Food and Drug Administration, Beijing, 100038, P. R. China.
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Zhang
authors.affiliation: Institute of Medicia Materia, China Academy of Chinese Medical Sciences, Beijing, 100700, P. R. China.
authors.forename: Jian-lu
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Institute of Medicia Materia, China Academy of Chinese Medical Sciences, Beijing, 100700, P. R. China.
authors.forename: Wan-dan
authors.initials: WD
authors.lastname: Wang
authors.affiliation: Zunyi Medical University, Zunyi, Guizhou 563003, P. R. China.
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, 100053, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Medicia Materia, China Academy of Chinese Medical Sciences, Beijing, 100700, P. R. China.
authors.forename: Ri-xin
authors.initials: RX
authors.lastname: Liang
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, P. R. China.
authors.forename: Xiao-bo
authors.initials: XB
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001158:Arteries / Q000187:drug effects / Q000378:metabolism; D050197:Atherosclerosis / Q000188:drug therapy* / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008055:Lipids; D008516:Medicine, Chinese Traditional / Q000379:methods; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D008055:Lipids; D016328:NF-kappa B