title: Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Cancer Cells.
abstract: The measurement of the transcriptional activity of the HH signaling pathway is widely used as an indication of pathway activation. Luciferase reporter assays are powerful tools to measure the specific ability of a transcription factor to bind to its consensus sequence and to activate transcription of target genes. Here, we describe a protocol to measure the transcriptional activity of the HH pathway in normal and cancer cells. This technique allows studying the activity of GLI transcription factors and their modulation by drugs and/or other factors.
authors.affiliation: Laboratory of Tumor Cell Biology, Core Research Laboratory - Istituto Toscano Tumori (CRL-ITT), Viale Pieraccini 6, 50139, Florence, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pandolfi
authors.forename: Barbara
authors.initials: B
authors.lastname: Stecca
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D015870:Gene Expression; D017930:Genes, Reporter; D053823:Hedgehog Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D053823:Hedgehog Proteins; D008156:Luciferases