title: In vitroandin vivoantitumor effects of 50 to 100-KDa components from B16 melanoma culture supernatant.
abstract: The development of immunological therapies for melanoma has been of considerable concern in recent years. Whole tumor cell lysates have been used to develop antitumor vaccines, but the effective components of the lysates have not been identified. In the present study, protein elements were purified from the B16 supernatant to analyze thein vitrochemotaxis towards mouse spleen lymphocytes using a Boyden chamber. Prior to establishing a B16 melanoma model, C57BL/6 mice were vaccinated with these proteins, and melanoma growth, tumor appearance time and behavioral changes were observed. Next, the cytotoxicity and subsets of the tumor infiltrating lymphocytes, and the histological characteristics of the melanoma were analyzed. The isolated purified fragments of B16 melanoma culture supernatant had strong antitumor effects. The possible antitumor mechanism was delineated, and was identified to possibly be through the activation of cluster of differentiation 8-positive T cells and the promotion of B16 cell differentiation. These methods will provide a novel insight into understanding antitumor immunological mechanisms and provide a potential avenue for immunotherapy.
authors.affiliation: Department of Pathophysiology, Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China.
authors.forename: Ying-Song
authors.initials: YS
authors.lastname: Qin
authors.affiliation: Department of Otolaryngological, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China.
authors.forename: X U
authors.initials: XU
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P.R. China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Zhang
keywords: chemotaxis; culture supernatant; immune killing; tumor infiltrating lymphocyte; vaccination