title: Chemical Constituents of Euonymus alatus (Thunb.) Sieb. and Their PTP1B and α-Glucosidase Inhibitory Activities.
abstract: Phytochemical study on the corks of Euonymus alatus resulted in the isolation of a novel 3-hydroxycoumarinflavanol (23), along with ten triterpenoids (1-10), ten phenolic derivatives (11-20), and two flavonoid glycosides (21 and 22). Their structures were determined by extensive 1D and 2D-nuclear magnetic resonance spectroscopic and mass spectrometry data analysis. Furthermore, their inhibitory effects against the protein tyrosine phosphatases 1B (PTP1B) and α-glucosidase enzyme activity were evaluated. Compounds 6, 7, 9, 15, 19, and 23 were non-competitive inhibitors, exhibiting most potency with IC50 values ranging from 5.6 ± 0.9 to 18.4 ± 0.3 µm, against PTP1B. Compound 3 (competitive), compounds 5 and 15 (mixed-competitive) displayed potent inhibition with IC50 values of 15.1 ± 0.7, 23.6 ± 0.6 and 14.8 ± 0.9 µm, respectively. Moreover, compounds 15, 20, and 23 exhibited potent inhibition on α-glucosidase with IC50 values of 10.5 ± 0.8, 9.5 ± 0.6, and 9.1 ± 0.5 µm, respectively. Thus, these active ingredients may have value as new lead compounds for the development of new antidiabetic agents.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Su-Yang
authors.initials: SY
authors.lastname: Jeong
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Phi-Hung
authors.initials: PH
authors.lastname: Nguyen
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Bing-Tian
authors.initials: BT
authors.lastname: Zhao
authors.affiliation: Department of Food Science & Nutrition, Pukyong National University, Busan, 608-737, Korea.
authors.forename: Md Yousof
authors.initials: MY
authors.lastname: Ali
authors.affiliation: Department of Food Science & Nutrition, Pukyong National University, Busan, 608-737, Korea.
authors.forename: Jae-Sue
authors.initials: JS
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Byung-Sun
authors.initials: BS
authors.lastname: Min
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology*; D029756:Euonymus; D005419:Flavonoids / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000737:chemistry; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010636:Phenols; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D000520:alpha-Glucosidases
keywords: Euonymus alatus; PTP1B inhibitors; flavanol; triterpenoids; α-glucosidase