title: CDKN3 knockdown reduces cell proliferation, invasion and promotes apoptosis in human ovarian cancer.
abstract: Cyclin-dependent kinase inhibitor 3 (CDKN3) has been reported to promote tumor genesis. The aim of this study is to investigate the possible mechanisms of silence of CDKN3 exerting the suppressive role on epithelial ovarian cancer (EOC). To study the potential function of CDKN3 enrolled in the regulation of ovarian tumor, we monitored the EOC cells SKOV3 and HO8910 behaviors including proliferation, cell cycle, apoptosis and invasion. First, we found that CDKN3 was frequently over-expressed in EOC. Functional studies showed that silence of CDKN3 inhibited cancer cell proliferation by promoting cell cycle progression in G1 phase, decreased cell invasion and promoted EOC cells apoptosis. Western blot analysis of CDKN3-silence cells revealed down-regulation of DNA-replication and cell cycle related proteins. And, a significant correlation level of CDKN3 was observed which has been demonstrated to be a novel oncogene. These findings indicated that CDKN3 might serve as a useful potential target for treatment of ovarian cancer.
authors.affiliation: Department of Ultrasound, Jiangxi Children's Hospital China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University China.
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University China.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University China.
authors.forename: Shan-Yu
authors.initials: SY
authors.lastname: Fang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University China.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Gao
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D004273:DNA, Neoplasm; C084429:CDKN3 protein, human; D054637:Dual-Specificity Phosphatases
keywords: CDKN3; apoptosis; invasion; ovarian cancer; proliferation