title: Serum Immunoproteomics Combined With Pathological Reassessment of Surgical Specimens Identifies TCP-1ζ Autoantibody as a Potential Biomarker in Thyroid Neoplasia.
abstract: BACKGROUND
  Current methods of preoperative diagnostics frequently fail to discriminate between benign and malignant thyroid neoplasms. In encapsulated follicular-patterned tumors (EnFPT), this discrimination is challenging even using histopathological analysis. Autoantibody response against tumor-associated antigens is a well-documented phenomenon with prominent diagnostic potential; however, autoantigenicity of thyroid tumors remains poorly explored.
  
  
  OBJECTIVE
  Objectives were exploration of tumor-associated antigen repertoire of thyroid tumors and identification of candidate autoantibody biomarkers capable of discrimination between benign and malignant thyroid neoplasms.
  
  
  METHODS
  Proteins isolated from FTC-133 cells were subjected to two-dimensional Western blotting using pooled serum samples of patients originally diagnosed with either papillary thyroid carcinoma (PTC) or EnFPT represented by apparently benign follicular thyroid adenomas, as well as healthy individuals. Immunoreactive proteins were identified using liquid chromatography-tandem mass-spectrometry. Pathological reassessment of EnFPT was performed applying nonconservative criteria for capsular invasion and significance of focal PTC nuclear changes (PTC-NCs). Recombinant T-complex protein 1 subunitζ (TCP-1ζ) was used to examine an expanded serum sample set of patients with various thyroid neoplasms (n = 89) for TCP-1ζ autoantibodies. All patients were included in tertiary referral centers.
  
  
  RESULTS
  A protein demonstrating a distinct pattern of EnFPT-specific seroreactivity was identified as TCP-1ζ protein. A subsequent search for clinicopathological correlates of TCP-1ζ seroreactivity revealed nonclassical capsular invasion or focal PTC-NC in all TCP-1ζ antibody-positive cases. Further studies in an expanded sample set confirmed the specificity of TCP-1ζ autoantibodies to malignant EnFPT.
  
  
  CONCLUSIONS
  We identified TCP-1ζ autoantibodies as a potential biomarker for presurgical discrimination between benign and malignant encapsulated follicular-patterned thyroid tumors. Our results suggest the use of nonconservative morphological criteria for diagnosis of malignant EnFPT in biomarker identification studies and provide a peculiar example of uncovering the diagnostic potential of a candidate biomarker using incorporation of pathological reassessment in the pipeline of immunoproteomic research.
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Belousov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Apollinariya V
authors.initials: AV
authors.lastname: Bogolyubova
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Yan S
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Abrosimov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Arthur T
authors.initials: AT
authors.lastname: Kopylov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Tvardovskiy
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Kirill V
authors.initials: KV
authors.lastname: Lanshchakov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Alexei Y
authors.initials: AY
authors.lastname: Sazykin
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Nina Y
authors.initials: NY
authors.lastname: Dvinskikh
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Yana I
authors.initials: YI
authors.lastname: Bobrovskaya
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Lilia S
authors.initials: LS
authors.lastname: Selivanova
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Evgeniy S
authors.initials: ES
authors.lastname: Shilov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Anton M
authors.initials: AM
authors.lastname: Schwartz
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Yuriy V
authors.initials: YV
authors.lastname: Shebzukhov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Severskaia
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Vladimir E
authors.initials: VE
authors.lastname: Vanushko
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Nedospasov
authors.affiliation: Department of Immunology (P.V.B., A.V.B., Y.S.K., A.Y.S., Y.I.B., E.S.S., S.A.N., D.V.K.) Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; Laboratory of Molecular Mechanisms of Immunity (A.V.B., S.A.N.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; Department of Pathomorphology (A.Y.A., L.S.S.), Endocrinology Research Center, 117036 Moscow, Russia; Acousto-Optical Research Center (A.Y.A.), National University of Science & Technology "MISIS," 119049 Moscow, Russia; Laboratory of Systems Biology (A.T.K.), Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Molecular Immunology (A.A.T., S.A.N.), A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; Department of Surgery (K.V.L., V.E.V.), Endocrinology Research Center, 117036 Moscow, Russia; Medical Radiology Research Center (N.Y.D., N.V.S.), 249036 Obninsk, Russia; Laboratory of Intracellular Signaling in Health and Disease (A.M.S., D.V.K.), Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia; German Rheumatism Research Center (Y.V.S., S.A.N.), a Leibniz Institute, 10117 Berlin, Germany; and Laboratory of Personalized Medicine (S.A.M.), Institute of Biomedical Chemistry, 119121 Moscow, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Kuprash
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002291:Carcinoma, Papillary / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D056404:Chaperonin Containing TCP-1 / Q000276:immunology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D013964:Thyroid Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; C533303:CCT6B protein, human; D056404:Chaperonin Containing TCP-1