title: Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production.
abstract: The BaM6PI gene encoding a mannose-6-phosphate isomerase (M6PI, EC 5.3.1.8) was cloned from Bacillus amyloliquefaciens DSM7 and overexpressed in Escherichia coli. The enzyme activity of BaM6PI was optimal at pH and temperature of 7.5 and 70°C, respectively, with a kcat/Km of 13,900 s-1 mM-1 for mannose-6-phosphate (M6P). The purified BaM6PI demonstrated the highest catalytic efficiency of all characterized M6PIs. Although M6PIs have been characterized from several other sources, BaM6PI is distinguished from other M6PIs by its wide pH range and high catalytic efficiency for M6P. The binding orientation of the substrate M6P in the active site of BaM6PI shed light on the molecular basis of its unusually high activity. BaM6PI showed 97% substrate conversion from M6P to fructose-6-phosphate demonstrating the potential for using BaM6PI in industrial applications.
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Sujan
authors.initials: S
authors.lastname: Sigdel
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Ranjitha
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Tae-Su
authors.initials: TS
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Li
authors.affiliation: BioNgene Co., Ltd, 10-1 Myungryun-Dong, Chongro-Gu, Seoul 110-521, Republic of Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: In-Won
authors.initials: IW
authors.lastname: Kim
authors.affiliation: Functional Food Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung 210-340, Republic of Korea.
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Jung
authors.affiliation: Functional Food Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung 210-340, Republic of Korea.
authors.forename: Cheol-Ho
authors.initials: CH
authors.lastname: Pan
authors.affiliation: Department of Materials Science and Engineering, Korea University, Anam-Dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea.
authors.forename: Yun Chan
authors.initials: YC
authors.lastname: Kang
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005636:Fructosephosphates / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008359:Mannose-6-Phosphate Isomerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020836:Protein Structure, Quaternary; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D005636:Fructosephosphates; D008670:Metals; C027618:fructose-6-phosphate; D008359:Mannose-6-Phosphate Isomerase