title: Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents.
abstract: BACKGROUND
  The rapid clinical translation of mesenchymal stem cells (MSC) has resulted in the development of cell-based strategies for multiple indications. Unfortunately one major barrier to widespread implementation of MSC-based therapies is the limited supply of fetal calf serum (FCS) used to expand cells to therapeutic numbers. Additionally, the xenogeneic element of fetal calf serum has been previously demonstrated to stimulate antibody mediated reactions and in some cases sensitization leading to anaphylaxis.
  
  
  METHODS
  XcytePLUS™ media, a human platelet lysate based product, was used to supplement the culture medium at 5, 7.5 and 10% and compared to fetal calf serum at 10%, for human umbilical cord MSC expansion. Properties of the expanded cells were investigated.
  
  
  RESULTS
  This study demonstrated equivalent or superior effects of human platelet lysate compared to standard FCS supplemented media, based on doubling rate, without loss of identity or function, as demonstrated with flow cytometry characterization. Differentiation into osteocytes, adipocytes and chondrocytes was comparable from cells expanded in either media supplement.
  
  
  CONCLUSIONS
  These data support the implementation of human platelet lysate supplemented media as an alternative to xenogeneic containing preparations which may lead to safer MSC products with therapeutic uses.
authors.affiliation: Medistem Panama, Inc., Building 221, City of Knowledge, Clayton, Panama City, Republic of Panama. nhriordan@gmail.com.
authors.forename: Neil H
authors.initials: NH
authors.lastname: Riordan
authors.affiliation: Medistem Panama, Inc., Building 221, City of Knowledge, Clayton, Panama City, Republic of Panama. marialauramadrigal@medistemresearch.com.|Indicasat AIP Panama, City of Knowledge, Rep. of Panama. marialauramadrigal@medistemresearch.com.|Acharya Nagarjuna University, Guntur, India. marialauramadrigal@medistemresearch.com.
authors.forename: Marialaura
authors.initials: M
authors.lastname: Madrigal
authors.affiliation: Amniotic Therapies, LLC, Farmers Branch, TX, USA. jasonreneau@testvalleyresearch.com.
authors.forename: Jason
authors.initials: J
authors.lastname: Reneau
authors.affiliation: Medistem Panama, Inc., Building 221, City of Knowledge, Clayton, Panama City, Republic of Panama. dekupeirok@medistemresearch.com.
authors.forename: Kathya
authors.initials: K
authors.lastname: de Cupeiro
authors.affiliation: Medistem Panama, Inc., Building 221, City of Knowledge, Clayton, Panama City, Republic of Panama. njimenez@medistemresearch.com.
authors.forename: Natalia
authors.initials: N
authors.lastname: Jiménez
authors.affiliation: Medistem Panama, Inc., Building 221, City of Knowledge, Clayton, Panama City, Republic of Panama. sruiz@medistemresearch.com.
authors.forename: Sergio
authors.initials: S
authors.lastname: Ruiz
authors.affiliation: Medistem Panama, Inc., Building 221, City of Knowledge, Clayton, Panama City, Republic of Panama. nsanchez@medistemresearch.com.
authors.forename: Nelsy
authors.initials: N
authors.lastname: Sanchez
authors.affiliation: Coma Research Institute, La Jolla, CA, USA. thomas.ichim@gmail.com.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ichim
authors.affiliation: University of Utah School of Medicine, Salt Lake City, UT, USA. fj74582@gmail.com.
authors.forename: Francisco
authors.initials: F
authors.lastname: Silva
authors.affiliation: University of Utah School of Medicine, Salt Lake City, UT, USA. Amit.Patel@hsc.utah.edu.
authors.forename: Amit N
authors.initials: AN
authors.lastname: Patel
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005434:Flow Cytometry; D006801:Humans; D007202:Indicators and Reagents; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010641:Phenotype; D059631:Wharton Jelly / Q000166:cytology
chemical_list: D016895:Culture Media, Serum-Free; D007202:Indicators and Reagents