title: CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators.
abstract: In the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location is associated to the risk of developing cardiovascular disease, insulin resistance, and other associated comorbidities. Under the above situation, the chronic local inflammation that characterizes obese adipose tissue, has acquired a major role on the pathogenesis of obesity. In this work, we have analyzed for the first time human obese VAT and SAT secretomes using an improved quantitative proteomic approach for the study of tissue secretomes, Comparison of Isotope-Labeled Amino acid Incorporation Rates (CILAIR). The use of double isotope-labeling-CILAIR approach to analyze VAT and SAT secretomes allowed the identification of location-specific secreted proteins and its differential secretion. Additionally to the very high percentage of identified proteins previously implicated in obesity or in its comorbidities, this approach was revealed as a useful tool for the study of the obese adipose tissue microenvironment including extracellular matrix (ECM) remodeling and inflammatory status. The results herein presented reinforce the fact that VAT and SAT depots have distinct features and contribute differentially to metabolic disease.
authors.affiliation: 1] Grupo Obesidómica, Instituto de Investigación Sanitaria de Santiago (IDIS), Xerencia de Xestión Integrada de Santiago (SERGAS), Santiago de Compostela, Spain [2] CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Spain.
authors.forename: Arturo
authors.initials: A
authors.lastname: Roca-Rivada
authors.affiliation: Unidad de Proteómica, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
authors.forename: Susana Belen
authors.initials: SB
authors.lastname: Bravo
authors.affiliation: Grupo Obesidómica, Instituto de Investigación Sanitaria de Santiago (IDIS), Xerencia de Xestión Integrada de Santiago (SERGAS), Santiago de Compostela, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Pérez-Sotelo
authors.affiliation: Unidad de Proteómica, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
authors.forename: Jana
authors.initials: J
authors.lastname: Alonso
authors.affiliation: 1] CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Spain [2] Laboratorio de Endocrinología Molecular y Celular, Instituto de Investigación Sanitaria de Santiago (IDIS), Xerencia de Xestión Integrada de Santiago (SERGAS), Spain.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Castro
authors.affiliation: Servicio de Cirugía General, Xerencia de Xestión Integrada de Santiago (SERGAS), Santiago de Compostela, Spain.
authors.forename: Iván
authors.initials: I
authors.lastname: Baamonde
authors.affiliation: Servicio de Cirugía General, Xerencia de Xestión Integrada de Santiago (SERGAS), Santiago de Compostela, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Baltar
authors.affiliation: 1] CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Spain [2] Laboratorio de Endocrinología Molecular y Celular, Instituto de Investigación Sanitaria de Santiago (IDIS), Xerencia de Xestión Integrada de Santiago (SERGAS), Spain.
authors.forename: Felipe F
authors.initials: FF
authors.lastname: Casanueva
authors.affiliation: 1] Grupo Obesidómica, Instituto de Investigación Sanitaria de Santiago (IDIS), Xerencia de Xestión Integrada de Santiago (SERGAS), Santiago de Compostela, Spain [2] CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Pardo
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism; D007333:Insulin Resistance / Q000502:physiology; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D009765:Obesity / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D018836:Inflammation Mediators