title: Mitochondrial ATP synthasome: Expression and structural interaction of its components.
abstract: Mitochondrial ATP synthase, ADP/ATP translocase (ANT), and inorganic phosphate carrier (PiC) are supposed to form a supercomplex called ATP synthasome. Our protein and transcript analysis of rat tissues indicates that the expression of ANT and PiC is transcriptionally controlled in accordance with the biogenesis of ATP synthase. In contrast, the content of ANT and PiC is increased in ATP synthase deficient patients' fibroblasts, likely due to a post-transcriptional adaptive mechanism. A structural analysis of rat heart mitochondria by immunoprecipitation, blue native/SDS electrophoresis, immunodetection and MS analysis revealed the presence of ATP synthasome. However, the majority of PiC and especially ANT did not associate with ATP synthase, suggesting that most of PiC, ANT and ATP synthase exist as separate entities.
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic; Charles University in Prague, First Faculty of Medicine, Kateřinská 32, 12108 Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Nůsková
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Mráček
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Mikulová
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Vrbacký
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Nikola
authors.initials: N
authors.lastname: Kovářová
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Kovalčíková
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Pecina
authors.affiliation: Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic. Electronic address: houstek@biomed.cas.cz.
authors.forename: Josef
authors.initials: J
authors.lastname: Houštěk
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D046911:Macromolecular Substances; D010710:Phosphates; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases; D025261:Mitochondrial Proton-Translocating ATPases
keywords: ATP synthasome; Mitochondria; Oxidative phosphorylation; Supercomplexes