title: Structural Basis of IgE Binding to α- and γ-Gliadins: Contribution of Disulfide Bonds and Repetitive and Nonrepetitive Domains.
abstract: Wheat products cause IgE-mediated allergies. The present study aimed to decipher the molecular basis of α- and γ-gliadin allergenicity. Gliadins and their domains, the repetitive N-terminal and the nonrepetitive C-terminal domains, were cloned and expressed in Escherichia coli. Their secondary structures and their IgE binding capacity were compared with those of natural proteins before and after reduction/alkylation. Allergenicity was evaluated with sera from patients who had a wheat food allergy or baker's asthma. The secondary structures of natural and recombinant proteins were slightly different. Compared with natural gliadins, recombinant proteins retained IgE binding but with reduced reactivity. Reduction/alkylation decreased IgE binding for both natural and recombinant gliadins. Although more continuous epitopes were identified in the N-terminal domains of α- and γ-gliadins, both the N-terminal and C-terminal domains contributed to IgE binding. As for other members of the prolamin superfamily, disulfide bonds appear to be of high importance for IgE binding.
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.|‡INRA, UMR1318, Institut Jean-Pierre Bourgin, INRA-AgroParisTech, F-78026 Versailles, France.
authors.forename: Hamza
authors.initials: H
authors.lastname: Mameri
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Brossard
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gaudin
authors.affiliation: ‡INRA, UMR1318, Institut Jean-Pierre Bourgin, INRA-AgroParisTech, F-78026 Versailles, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Gohon
authors.affiliation: §Service de Pneumologie et d'Allergologie Pédiatrique, Groupe hospitalier Necker, F-75015 Paris, France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Paty
authors.affiliation: ΔService d'Allergologie, CH Epinal, F-88021 Epinal, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Beaudouin
authors.affiliation: ΔService d'Allergologie, CH Epinal, F-88021 Epinal, France.
authors.forename: Denise-Anne
authors.initials: DA
authors.lastname: Moneret-Vautrin
authors.affiliation: ⊥Unité d'Allergologie Générale et de Pneumologie, CHU d'Angers, F-49933 Angers, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Drouet
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Solé
authors.affiliation: #Synchrotron Soleil, Saint-Aubin, F-91192 Gif-sur-Yvette, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Wien
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.
authors.forename: Roberta
authors.initials: R
authors.lastname: Lupi
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.
authors.forename: Colette
authors.initials: C
authors.lastname: Larré
authors.affiliation: ‡INRA, UMR1318, Institut Jean-Pierre Bourgin, INRA-AgroParisTech, F-78026 Versailles, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Snégaroff
authors.affiliation: †INRA, UR1268 BIA (Biopolymers, Interactions, Assemblies), F-44316 Nantes, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Denery-Papini
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins; D002942:Circular Dichroism; D004220:Disulfides / Q000737:chemistry*; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins; D005903:Gliadin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006867:Hydrolases; D007073:Immunoglobulin E / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012091:Repetitive Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014908:Triticum / Q000737:chemistry; D021182:Wheat Hypersensitivity / Q000097:blood / Q000276:immunology
chemical_list: D000485:Allergens; D001425:Bacterial Outer Membrane Proteins; D004220:Disulfides; D000939:Epitopes; D029968:Escherichia coli Proteins; C482905:OmpX protein, E coli; D007073:Immunoglobulin E; D005903:Gliadin; D006867:Hydrolases
keywords: IgE epitopes; recombinant wheat allergens; synchrotron radiation circular dichroism; wheat allergy; wheat gliadins