title: Structurally Diverse Polyamines: Solid-Phase Synthesis and Interaction with DNA.
abstract: A versatile solid-phase approach based on peptide chemistry was used to construct four classes of structurally diverse polyamines with modified backbones: linear, partially constrained, branched, and cyclic. Their effects on DNA duplex stability and structure were examined. The polyamines showed distinct activities, thus highlighting the importance of polyamine backbone structure. Interestingly, the rank order of polyamine ability for DNA compaction was different to that for their effects on circular dichroism and melting temperature, thus indicating that these polyamines have distinct effects on secondary and higher-order structures of DNA.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan). umezawa@phar.nagoya-cu.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Umezawa
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan).
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Horai
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan).
authors.forename: Yuki
authors.initials: Y
authors.lastname: Imamura
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan).
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawakubo
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan).
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakahira
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan).
authors.forename: Nobuki
authors.initials: N
authors.lastname: Kato
authors.affiliation: Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, 610-0394 (Japan).
authors.forename: Akira
authors.initials: A
authors.lastname: Muramatsu
authors.affiliation: Research Organization of Science and Technology, Ritsumeikan University, Kusatsu 525-8577 (Japan).
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yoshikawa
authors.affiliation: Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, 610-0394 (Japan).
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 (Japan). higuchi@phar.nagoya-cu.ac.jp.
authors.forename: Tsunehiko
authors.initials: T
authors.lastname: Higuchi
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D015394:Molecular Structure; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D060327:Solid-Phase Synthesis Techniques
chemical_list: D011073:Polyamines; D004247:DNA
keywords: DNA structures; amines; peptides; single-molecule studies; solid-phase synthesis