title: Controlled delivery of dopamine hydrochloride using surface modified carbon dots for neuro diseases.
abstract: Delivery of therapeutic agents using water-soluble, highly biocompatible Carbon dots (C-dots) is an efficient strategy to control drug release under physiological milieu. Dopamine hydrochloride (DA), the most important inotropic vasopressor agent used in neurological diseases. In our study DA is anchored to water-soluble carbon dots for controlled release under mimicked in vitro physiological conditions. The tenure of the DA release at pH 7.4 was greatly extended to 60 h for C-dots-DA, in comparison with the control DA alone. The statistical calculation was used to comprehend the release pattern of the DA, which exhibited the pattern of Hixson-Crowell model of release. In order to understand the impact of the C-dots-DA conjugate under physiological conditions, Neuro 2A cells were taken under consideration. The conjugate C-dots-DA was found to be biocompatible against Neuro 2A cells. The survival rate was found to be 74% at maximum concentration of 9 μg mL(-1). In vivo toxicity was studied using thin section of tissues after staining with Hematoxyline and Eosin Yellow (H&E). As per microscopic observations, conjugates did not inflict any anatomical distortions or hostile effects on tissues. Body weight of mice was also taken into consideration after injecting 20 μg mL(-1) of nano-conjugates via tail vein. The impact of nano-conjugate on body weight was found to be negligible after 45 days of observation.
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan.
authors.forename: M Shahnawaz
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan.
authors.forename: Sunil
authors.initials: S
authors.lastname: Pandey
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan.
authors.forename: Abou
authors.initials: A
authors.lastname: Talib
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan.
authors.forename: Mukesh Lavkush
authors.initials: ML
authors.lastname: Bhaisare
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institue of Medical Science and Technology, National Sun Yat-Sen University, 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan. Electronic address: hwu@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001835:Body Weight; D002244:Carbon / Q000737:chemistry*; D002460:Cell Line; D004298:Dopamine / Q000008:administration & dosage* / Q000627:therapeutic use / Q000633:toxicity; D004337:Drug Carriers; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009422:Nervous System Diseases / Q000188:drug therapy*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D002244:Carbon; D004298:Dopamine
keywords: C-dots; Dopamine hydrochloride (DA); Drug delivery; Hixson–Crowell; Neuro 2A cells