title: Noncationic Rigid and Anisotropic Coiled-Coil Proteins Exhibit Cell-Penetration Activity.
abstract: Numerous cationic peptides that penetrate cells have been studied intensively as drug delivery system carriers for cellular delivery. However, cationic molecules tend to be cytotoxic and cause inflammation, and their stability in the blood is usually low. We have previously demonstrated that a rigid and fibrous cationic coiled-coil protein exhibited cell-penetrating ability superior to that of previously reported cell-penetrating peptides. Making use of structural properties, here we describe the cell-penetrating activity of a rigid and fibrous coiled-coil protein with a noncationic surface. A fibrous coiled-coil protein of pI 6.5 penetrated 100% of the cells tested in vitro at a concentration of 500 nM, which is comparable to that of previously reported cell-penetrating peptides. We also investigated the effect of cell-strain dependency and short-term cytotoxicity.
authors.affiliation: ‡Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Nakayama
authors.affiliation: ‡Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.|§Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506, Japan.
authors.forename: Kyoji
authors.initials: K
authors.lastname: Hagiwara
authors.affiliation: ‡Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.affiliation: ‡Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.|∥Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0021, Japan.
authors.forename: Kuniharu
authors.initials: K
authors.lastname: Ijiro
authors.affiliation: ‡Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Osada
authors.affiliation: ‡Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Sano
mesh_terms: D000595:Amino Acid Sequence; D016880:Anisotropy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D008969:Molecular Sequence Data; D013329:Structure-Activity Relationship
chemical_list: D057846:Cell-Penetrating Peptides