title: PBEF promotes the apoptosis of pulmonary microvascular endothelial cells and regulates the expression of inflammatory factors and AQP1 through the MAPK pathways.
abstract: Pre-B cell colony-enhancing factor (PBEF) has been shown to have a variety of biological functions. Studies have proven that PBEF plays a functional role in acute lung injury (ALI). Therefore, in this study, we aimed to confirm the importance of PBEF in ALI. The effects of PBEF overexpression on the apoptosis of human pulmonary microvascular endothelial cells (HPMECs) were analyzed by flow cytometry, and the results indicated that PBEF promoted the apoptosis of HPMECs, which aggravated the development of ALI. Comparative experiments involving increasing and decreasing PBEF expression demonstrated that PBEF promoted the expression of inflammatory factors, such as interleukin (IL)‑1β, IL‑6 and IL‑8 in the HPMECs , thus intensifying the inflammatory response. PBEF also inhibited the expression of aquaporin 1 (AQP1), which caused a dysfunction and imbalance in water transport. Moreover, we also found that tumor necrosis factor (TNF)‑α promoted the expression of PBEF in the HPMECs. After blocking the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways, we found that PBEF regulated the expression of inflammatory factors and AQP1, mainly through the MAPK pathways. Taken together, these results demonstrate that the increase in intracellular PBEF expression promoted the apoptosis of HPMECs and the expression of inflammatory factors and thus enhanced the inflammatory response and inhibited the expression of AQP1, which resulted in abnormal water transport, diminishing the regulatory effects of AQP1 on water transport.
authors.affiliation: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Guang-Feng
authors.initials: GF
authors.lastname: Ming
authors.affiliation: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Ma
authors.affiliation: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Dao-Miao
authors.initials: DM
authors.lastname: Xu
authors.affiliation: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Yu-Hang
authors.initials: YH
authors.lastname: Ai
authors.affiliation: Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zan-Hua
authors.initials: ZH
authors.lastname: Shi
mesh_terms: D017209:Apoptosis; D051398:Aquaporin 1 / Q000276:immunology*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D018836:Inflammation Mediators / Q000276:immunology*; D007378:Interleukins / Q000276:immunology; D008168:Lung / Q000098:blood supply*; D020935:MAP Kinase Signaling System; D055806:Microvessels / Q000276:immunology* / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D007378:Interleukins; D051398:Aquaporin 1; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human