title: Molecular characterization of tea mosquito bug from tea growing regions of India.
abstract: The tea mosquito bug, Helopeltis (Hemiptera: Miridae), is an insidious pest that poses a significant economical threat to tea plantations. As a basic first step to control this pest is authentic identification, but the inability to determine morphological characters of Helopeltis species makes this process very difficult. DNA barcoding is a reliable alternative to traditional morphological identification of this pest. Since tea is cultivated in different parts of the country, an attempt was made to molecular characterization of Helopeltis. This is the first report on molecular identification and diversity characterization of Helopeltis collected from tea growing regions of southern and north India, using cytochrome c oxidase subunit I (COI) gene of mitochondrial (mt) DNA. Beginning with the molecular identification of this pest is essential to start an effective pest management strategy, and will provide basic information for diffusion pattern, population dynamics and chemical application.
authors.affiliation: a Plant Physiology and Biotechnology Division, UPASI Tea Research Institute , Valparai, Coimbatore , Tamil Nadu , India and.
authors.forename: M
authors.initials: M
authors.lastname: Suganthi
authors.affiliation: a Plant Physiology and Biotechnology Division, UPASI Tea Research Institute , Valparai, Coimbatore , Tamil Nadu , India and.
authors.forename: K N
authors.initials: KN
authors.lastname: Chandrashekara
authors.affiliation: a Plant Physiology and Biotechnology Division, UPASI Tea Research Institute , Valparai, Coimbatore , Tamil Nadu , India and.|b Department of Plant Biotechnology , P.S.G Arts and Science College , Coimbatore , Tamil Nadu , India.
authors.forename: S
authors.initials: S
authors.lastname: Arvinth
authors.affiliation: a Plant Physiology and Biotechnology Division, UPASI Tea Research Institute , Valparai, Coimbatore , Tamil Nadu , India and.
authors.forename: R
authors.initials: R
authors.lastname: Raj Kumar
mesh_terms: D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics*; D020062:Heteroptera / Q000145:classification* / Q000235:genetics; D007194:India; D019476:Insect Proteins / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013662:Tea / Q000469:parasitology*
chemical_list: D019476:Insect Proteins; D013662:Tea; D003576:Electron Transport Complex IV
keywords: COI; DNA barcoding; Helopeltis; mtDNA