title: Association of Anti-GT1a Antibodies with an Outbreak of Guillain-Barré Syndrome and Analysis of Ganglioside Mimicry in an Associated Campylobacter jejuni Strain.
abstract: An outbreak of Guillain-Barré syndrome (GBS), subsequent to Campylobacter jejuni enteritis, occurred in China in 2007. Serum anti-ganglioside antibodies were measured in GBS patients and controls. Genome sequencing was used to determine the phylogenetic relationship among three C. jejuni strains from a patient with GBS (ICDCCJ07001), a patient with gastroenteritis (ICDCCJ07002) and a healthy carrier (ICDCCJ07004), which were all associated with the outbreak. The ganglioside-like structures of the lipo-oligosaccharides of these strains were determined by mass spectrometry. Seventeen (53%) of the GBS patients had anti-GT1a IgG antibodies. GT1a mimicry was found in the lipo-oligosaccharides of strain ICDCCJ07002 and ICDCCJ07004; but a combination of GM3/GD3 mimics was observed in ICDCCJ07001, although this patient had anti-GT1a IgG antibodies. A single-base deletion in a glycosyltransferase gene caused the absence of GT1a mimicry in ICDCCJ07001. The phylogenetic tree showed that ICDCCJ07002 and ICDCCJ07004 were genetically closer to each other than to ICDCCJ07001. C. jejuni, bearing a GT1a-like lipo-oligosaccharide, might have caused the GBS outbreak and the loss of GT1a mimicry may have helped ICDCCJ07001 to survive in the host.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, 102206; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China, 310003.
authors.forename: Maojun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Human Health Therapeutics, National Research Council Canada, Ottawa, K1A 0R6, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Gilbert
authors.affiliation: National Neuroscience Institute, Singapore, Singapore, 308433.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yuki
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, 102206.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Cao
authors.affiliation: Human Health Therapeutics, National Research Council Canada, Ottawa, K1A 0R6, Canada.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, 102206.
authors.forename: Hongying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Office for Disease Control and Emergency Response, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, 102206.
authors.forename: Fanliang
authors.initials: F
authors.lastname: Meng
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, 102206; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China, 310003.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000942:Antigens, Bacterial / Q000097:blood / Q000235:genetics / Q000276:immunology*; D002169:Campylobacter Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D016123:Campylobacter jejuni / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005732:Gangliosides / Q000097:blood / Q000235:genetics / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000097:blood / Q000235:genetics / Q000276:immunology; D008297:Male; D018716:Molecular Mimicry / Q000276:immunology*; D010802:Phylogeny; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D005732:Gangliosides; D008070:Lipopolysaccharides