title: Contrasting effects of G1.2/G2.2 and SOF1/SOF2 embryo culture media on pre- and post-implantation development of non-transgenic and transgenic cloned goat embryos.
abstract: This study compared the efficiency of two embryo culture media (SOF1/SOF2 and G1.2/G2.2) for pre- and post-implantation development of somatic cell nuclear transfer goat embryos derived from non-transgenic and transgenic (for htPA and hrcfIX genes) fibroblasts. Despite similar cleavage rates, G1.2/G2.2 supported significantly higher blastocyst development than SOF1/SOF2 (30-35% versus 21%; P < 0.05), irrespective of cell transgenesis. However, following embryo transfer, pregnancy outcomes (establishment, full-term development and live birth) were all significantly higher (P < 0.05) for embryos developed in SOF1/SOF2 versus G1.2/G2.2. Gene expression profiling of 17 developmentally important genes revealed that: (i) SOX2, FOXD3, IFNT, FZD, FGFR4, ERK1, GCN5, PCAF, BMPR1, SMAD5, ALK4, CDC25 and LIFR were significantly induced in blastocysts developed in SOF1/SOF2 but not G1.2/G2.2; (ii) OCT4, CTNNB and CDX2 were similarly expressed in both groups; and (iii) AKT was significantly higher in G1.2/G2.2 than SOF1/SOF2 (P < 0.05). Following IVF, although blastocyst development in G1.2/G2.2 was significantly higher than SOF1/SOF2 counterparts, the majority of assessed genes were similarly expressed in blastocysts developed in both groups. It was concluded that the long-term programming effects of embryo culture medium and/or embryo production method may irreversibly affect post-implantation development of cloned embryos through defined molecular pathways.
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Sayed Morteza
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hajian
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Somayyeh
authors.initials: S
authors.lastname: Ostadhosseini
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Forouzanfar
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Abedi
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Farnoosh
authors.initials: F
authors.lastname: Jafarpour
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Medicine, ACECR, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Gourabi
authors.affiliation: Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, P.O. Box: 19395-4644, Tehran, Iran.
authors.forename: Abdol Hossein
authors.initials: AH
authors.lastname: Shahverdi
authors.affiliation: Department of Reproductive Imaging at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Vosough
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Ghanaie
authors.affiliation: Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic address: mh.nasr-esfahani@royaninstitute.org.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000187:drug effects; D019976:Cloning, Organism; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D006041:Goats; D011247:Pregnancy; D011256:Pregnancy Outcome
chemical_list: D003470:Culture Media
keywords: G1.2/G2.2; SOF1/SOF2; embryo development; gene expression; goat; nuclear transfer