title: Comparative analysis of markers of cell proliferation in canine mast cell tumours according to current classifications.
abstract: The study aimed at immunohistochemical analysis of various markers of cell proliferation and comparison of the results with canine mast cell tumours grading systems according to the Patnaik and Kiupel. Tissue sections were stained using classical technique with haematoxylin and eosin, and immunohistochemical studies were performed with Ki-67, PCNA and MCM-3 antibodies. Additionally the mitotic index was assessed. Statistical analysis including rank correlation Spearman's and ANOVA Friedman analysis was performed. The significance was set at p<0.05. Expression of all examined antigens was detected. The results obtained allow concluding that there is a strong relationship between all the cell markers. However, due to the very strong response and positive reaction in the majority of tumours PCNA is not recommended as a prognostic indicator. Ki-67 and MCM-3 can be successfully used in the evaluation of canine mast cell tumours.
authors.forename: M
authors.initials: M
authors.lastname: Kandefer-Gola
authors.forename: J A
authors.initials: JA
authors.lastname: Madej
authors.forename: S
authors.initials: S
authors.lastname: Dzimira
authors.forename: M
authors.initials: M
authors.lastname: Nowak
authors.forename: I
authors.initials: I
authors.lastname: Janus
authors.forename: R
authors.initials: R
authors.lastname: Ciaputa
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D049109:Cell Proliferation; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D034801:Mastocytoma / Q000378:metabolism*; D064126:Minichromosome Maintenance Complex Component 3 / Q000235:genetics / Q000378:metabolism; D060787:Neoplasm Grading; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D064126:Minichromosome Maintenance Complex Component 3