title: Cocaine self-administration in Wistar-Kyoto rats: a behavioral and biochemical analysis.
abstract: Depression and cocaine abuse disorders are common concurrent diagnoses. In the present study, we employed Wistar-Kyoto (WKY) rats that showed a depressive-like phenotype to study intravenous cocaine self-administration and extinction/reinstatement procedures. We also investigated the basal tissue level of neurotransmitters, their metabolites and plasma corticosterone (CORT) concentrations in WKY rats, bulbectomized (OBX) rats, and control rats. The WKY rats exhibited an attenuation of the cocaine-associated lever presses and cocaine intake during the acquisition/maintenance of cocaine self-administration only under specific conditions. Active lever presses exhibited by the WKY rats and control animals did not differ during the extinction training and cocaine-seeking behaviors. The WKY rats demonstrated alterations in the basal levels of dopamine, norepinephrine, and serotonin in selected brain structures involved in depression and drug addiction. The changes in the level of neurotransmitters in these animals refer not only to the control (Wistar) rats but also to bulbectomized animals, which represent another depression model. Furthermore, we identified unchanged levels of CORT in the WKY and OBX rats during the light phase and free-stress conditions. This finding suggests that WKY rats should not be used to investigate the co-occurrence of depression and cocaine addiction, as this rat strain does not show an enhanced risk of relapse.
authors.affiliation: Laboratory of Drug Addiction Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Jastrzębska
authors.affiliation: Laboratory of Drug Addiction Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Frankowska
authors.affiliation: Laboratory of Drug Addiction Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Szumiec
authors.affiliation: Laboratory of Drug Addiction Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sadakierska-Chudy
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Haduch
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Jagiellonian University, Kraków, Medyczna 9, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Smaga
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Jagiellonian University, Kraków, Medyczna 9, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Bystrowska
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland.
authors.forename: Wladyslawa A
authors.initials: WA
authors.lastname: Daniel
authors.affiliation: Laboratory of Drug Addiction Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Smętna 12, Poland; Department of Toxicology, Faculty of Pharmacy, Jagiellonian University, Kraków, Medyczna 9, Poland. Electronic address: mal.fil@if-pan.krakow.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Filip
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects*; D003042:Cocaine / Q000008:administration & dosage*; D003216:Conditioning, Operant / Q000187:drug effects*; D003345:Corticosterone / Q000097:blood; D004305:Dose-Response Relationship, Drug; D055664:Electrochemical Techniques; D005108:Extinction, Psychological / Q000187:drug effects*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000378:metabolism; D009830:Olfactory Bulb / Q000502:physiology / Q000601:surgery; D051381:Rats; D011921:Rats, Inbred WKY; D017208:Rats, Wistar; D012055:Reinforcement Schedule; D012646:Self Administration; D013550:Swimming / Q000523:psychology
chemical_list: D000779:Anesthetics, Local; D018377:Neurotransmitter Agents; D003042:Cocaine; D003345:Corticosterone
keywords: Bulbectomy; Cocaine; Depression; Neurotransmitters; Self-administration; Wistar Kyoto rats