title: Determination of Doxepin in human plasma using cloud-point extraction with high-performance liquid chromatography and ultraviolet detection.
abstract: A new straightforward method based on cloud-point extraction has been developed, optimized, and validated for the determination of doxepin in human plasma by high-performance liquid chromatography separation and UV detection. The nonionic surfactant Triton X-114 was chosen as the extraction solvent. Chromatography separation was performed on a μBondapakRC18column (4.6 mm id × 300 mm, 3 μm particle size), which was used for isocratic elution at a detection wavelength of 289 nm. Under the optimum conditions, the linear range of doxepin in human plasma was 0.1-0.9 μg/mL. Also, the detection limit, preconcentration factor, and enrichment factor were 0.08 μg/mL, 50, and 49.0, respectively.
authors.affiliation: Department of Chemistry, Payame Noor University, Tehran, I. R. of Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Baezzat
authors.affiliation: Department of Chemistry, Payame Noor University, Tehran, I. R. of Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Banavand
authors.affiliation: Department of Chemistry, Payame Noor University, Tehran, I. R. of Iran.
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Tabandeh
authors.affiliation: Faculty of Science, Department of Applied Chemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Amiri
authors.affiliation: Department of Chemistry, Payame Noor University, Tehran, I. R. of Iran.
authors.forename: Rogheye
authors.initials: R
authors.lastname: Pourghobadi
keywords: Cloud-point extraction; Doxepin; High-performance liquid chromatography; Triton X-114