title: A Phenylpropanoid Glycoside as a Calcineurin Inhibitor Isolated from Magnolia obovata Thunb.
abstract: To identify plant-derived cell signaling inhibitors with antifungal properties, a twocomponent screening system using both wild-type Cryptococcus neoformans and a calcineurin mutant was employed owing to their counter-regulatory actions on the Hog1 mitogenactivated protein kinase and calcineurin pathways. Of the 2,000 plant extracts evaluated, a single bioactive compound from M. obovata Thunb. was found to act specifically on the calcineurin pathway of C. neoformans. This compound was identified as magnoloside A, and had potent antifungal activities against various Cryptococcus strains with minimum inhibitory concentration values ranging from 1.0 to 4.0 μg/ml.
authors.affiliation: Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Won Jeong
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Jae Sun
authors.initials: JS
authors.lastname: Moon
authors.affiliation: Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Sung In
authors.initials: SI
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Bahn
authors.affiliation: Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Hanna
authors.initials: H
authors.lastname: Lee
authors.affiliation: Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kang
authors.affiliation: Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Heung Mook
authors.initials: HM
authors.lastname: Shin
authors.affiliation: Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Sung Uk
authors.initials: SU
authors.lastname: Kim
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism*; D001688:Biological Products / Q000302:isolation & purification / Q000378:metabolism*; D065095:Calcineurin Inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000201:enzymology; D004353:Drug Evaluation, Preclinical; D006027:Glycosides / Q000302:isolation & purification / Q000378:metabolism*; D031566:Magnolia / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D001688:Biological Products; D065095:Calcineurin Inhibitors; D006027:Glycosides; D010936:Plant Extracts
keywords: Antifungal activity; Calcineurin inhibitor; Cryptococcus neoformans; Signaling pathway; Two-component system