title: Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014.
abstract: A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.
authors.forename: Charles Y
authors.initials: CY
authors.lastname: Chiu
authors.forename: Vanessa
authors.initials: V
authors.lastname: Bres
authors.forename: Guixia
authors.initials: G
authors.lastname: Yu
authors.forename: David
authors.initials: D
authors.lastname: Krysztof
authors.forename: Samia N
authors.initials: SN
authors.lastname: Naccache
authors.forename: Deanna
authors.initials: D
authors.lastname: Lee
authors.forename: Jacob
authors.initials: J
authors.lastname: Pfeil
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Linnen
authors.forename: Susan L
authors.initials: SL
authors.lastname: Stramer
mesh_terms: D001782:Blood Donors / Q000706:statistics & numerical data / Q000600:supply & distribution*; D065632:Chikungunya Fever / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics; D002646:Chikungunya virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D005820:Genetic Testing / Q000379:methods* / Q000706:statistics & numerical data; D023281:Genomics; D006801:Humans; D011647:Puerto Rico / Q000453:epidemiology
keywords: ViroChip microarray; blood donor; chikungunya virus; next-generation sequencing; transcription-mediated amplification; transfusion-transmitted infection; viruses; whole-genome sequencing