title: Simultaneous analysis of D-alanine, D-aspartic acid, and D-serine using chiral high-performance liquid chromatography-tandem mass spectrometry and its application to the rat plasma and tissues.
abstract: A highly sensitive and selective chiral LC-MS/MS method for D-alanine, D-aspartic acid and D-serine has been developed using the precolumn derivatization reagents, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AccQ-Tag) or p-N,N,N-trimethylammonioanilyl N'-hydroxysuccinimidyl carbamate iodide (TAHS). The thus N-tagged enantiomers of the derivatized amino acids were nicely separated within 20min using the cinchona alkaloid-based zwittterionic ion-exchange type enantioselective column, Chiralpak ZWIX(+). The selected reaction monitoring was applied for detecting the target d-amino acids in biological matrices. By using the present chiral LC-MS/MS method, the three d-amino acids and their l-forms could be simultaneously determined in the range of 0.1-500nmol/mL. Finally, the technique was successfully applied to rat plasma and tissue samples.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan.
authors.forename: Sachise
authors.initials: S
authors.lastname: Karakawa
authors.affiliation: Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Shimbo
authors.affiliation: Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Yamada
authors.affiliation: Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan.
authors.forename: Toshimi
authors.initials: T
authors.lastname: Mizukoshi
authors.affiliation: Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyano
authors.affiliation: Shiseido Co., Ltd., 1-6-2Higashi-shimbashi, Minato-ku, Tokyo 105-8310, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Mita
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Währingerstrasse 38, 1090 Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lindner
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: hamase@phar.kyushu-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Hamase
mesh_terms: D000409:Alanine / Q000032:analysis* / Q000097:blood; D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D026603:D-Aspartic Acid / Q000032:analysis* / Q000097:blood; D008297:Male; D009928:Organ Specificity; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012694:Serine / Q000032:analysis* / Q000097:blood; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014781:Viscera / Q000378:metabolism
chemical_list: D012694:Serine; D026603:D-Aspartic Acid; D000409:Alanine
keywords: Chiral separation; LC-MS/MS; d-Amino acids