title: Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath.
abstract: A perfluorocarbon nanodroplet formulation is shown to be an effective cavitation enhancement agent, enabling rapid and consistent fragmentation of genomic DNA in a standard ultrasonic water bath. This nanodroplet-enhanced method produces genomic DNA libraries and next-generation sequencing results indistinguishable from DNA samples fragmented in dedicated commercial acoustic sonication equipment, and with higher throughput. This technique thus enables widespread access to fast bench-top genomic DNA fragmentation.
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina, Chapel Hill, North Carolina, and North Carolina State University, Raleigh, North Carolina, United States of America.
authors.forename: Sandeep K
authors.initials: SK
authors.lastname: Kasoji
authors.affiliation: Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Samantha G
authors.initials: SG
authors.lastname: Pattenden
authors.affiliation: Department of Genetics, High Throughput Sequencing Facility, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Ewa P
authors.initials: EP
authors.lastname: Malc
authors.affiliation: Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Chatura N
authors.initials: CN
authors.lastname: Jayakody
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina, Chapel Hill, North Carolina, and North Carolina State University, Raleigh, North Carolina, United States of America; Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: James K
authors.initials: JK
authors.lastname: Tsuruta
authors.affiliation: Department of Genetics, High Throughput Sequencing Facility, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Piotr A
authors.initials: PA
authors.lastname: Mieczkowski
authors.affiliation: Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: William P
authors.initials: WP
authors.lastname: Janzen
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina, Chapel Hill, North Carolina, and North Carolina State University, Raleigh, North Carolina, United States of America.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Dayton
mesh_terms: D053938:DNA Fragmentation / Q000528:radiation effects*; D004271:DNA, Fungal; D045423:Microbubbles; D013010:Sonication / Q000295:instrumentation / Q000379:methods*
chemical_list: D004271:DNA, Fungal