title: The Role of Intermittent Hypoxia on the Proliferative Inhibition of Rat Cerebellar Astrocytes.
abstract: Sleep apnea syndrome, characterized by intermittent hypoxia (IH), is linked with increased oxidative stress. This study investigates the mechanisms underlying IH and the effects of IH-induced oxidative stress on cerebellar astrocytes. Rat primary cerebellar astrocytes were kept in an incubator with an oscillating O2 concentration between 20% and 5% every 30 min for 1-4 days. Although the cell loss increased with the duration, the IH incubation didn't induce apoptosis or necrosis, but rather a G0/G1 cell cycle arrest of cerebellar astrocytes was noted. ROS accumulation was associated with cell loss during IH. PARP activation, resulting in p21 activation and cyclin D1 degradation was associated with cell cycle G0/G1 arrest of IH-treated cerebellar astrocytes. Our results suggest that IH induces cell loss by enhancing oxidative stress, PARP activation and cell cycle G0/G1 arrest in rat primary cerebellar astrocytes.
authors.affiliation: Department of Research, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
authors.forename: Sheng-Chun
authors.initials: SC
authors.lastname: Chiu
authors.affiliation: Physiological and Anatomical Medicine, School of Medicine, Tzu Chi University, Hualien, Taiwan.
authors.forename: Yu-Jou
authors.initials: YJ
authors.lastname: Lin
authors.affiliation: Department of Ophthalmology, Mackay Memorial Hospital, Hsinchu, Taiwan.
authors.forename: Sung-Ying
authors.initials: SY
authors.lastname: Huang
authors.affiliation: Institute of Medical Sciences, School of Medicine, Tzu Chi University, Hualien, Taiwan.
authors.forename: Chih-Feng
authors.initials: CF
authors.lastname: Lien
authors.affiliation: Tzu Chi Stem Cells Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
authors.forename: Shee-Ping
authors.initials: SP
authors.lastname: Chen
authors.affiliation: Institute of Medical Sciences, School of Medicine, Tzu Chi University, Hualien, Taiwan; Department of Medical Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
authors.forename: Cheng-Yoong
authors.initials: CY
authors.lastname: Pang
authors.affiliation: PhD program in Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Physiological and Anatomical Medicine, School of Medicine, Tzu Chi University, Hualien, Taiwan; Department of Physiology, School of Medicine, Tzu Chi University, Hualien, Taiwan.
authors.forename: Kun-Ta
authors.initials: KT
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D000067856:Poly(ADP-ribose) Polymerase Inhibitors / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000187:drug effects
chemical_list: D000975:Antioxidants; D050759:Cyclin-Dependent Kinase Inhibitor p21; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D017382:Reactive Oxygen Species; D011065:Poly(ADP-ribose) Polymerases