title: Cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials.
abstract: According to the cost of carbon substrate for producing biopolymer of polyhydroxyalkanoates (PHAs) still has a barrier to extend in an industrial scale. The aim of this work was to evaluate the defined PHAs media containing the agricultural raw materials of pineapple and sugarcane to produce PHAs by Bacillus strain. Batch fermentation was carried out in flask scale to compare the efficiency of defined media using statistical methodology. The defined medium 8 of pineapple was achieved the highest PHAs concentration and productivity (1.86 g/L and 0.077 g/Lh). The effect of pH and aeration was extensively studied in a fermentor. The results were revealed that PHAs production would be increased from the condition of uncontrolled pH and at higher aeration rate. The extracted PHAs clearly showed in a homopolymer structure of poly(3-hydroxybutyrate) (PHB) with melting temperature (Tm) of 172°C 54.39% crystallinity.
authors.affiliation: Graduate School of Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Waranya
authors.initials: W
authors.lastname: Suwannasing
authors.affiliation: Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Ube 755-8611, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Imai
authors.affiliation: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: pakawadee.kku@gmail.com.
authors.forename: Pakawadee
authors.initials: P
authors.lastname: Kaewkannetra
mesh_terms: D000383:Agriculture; D031230:Ananas; D001407:Bacillus / Q000378:metabolism; D019149:Bioreactors; D001709:Biotechnology / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry / Q000191:economics*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry / Q000191:economics; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; D054813:Polyhydroxyalkanoates; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Bacillus sp.; Pineapple; Poly(3-hydroxybutyrate); Statistical test; Sugar-based material