title: Lentivirus-mediated silencing of USO1 inhibits cell proliferation and migration of human colon cancer cells.
abstract: Belonging to the tether factors family, USO1 (vesicle transport factor) plays a critical role in endoplasmic reticulum-Golgi trafficking and vesicular transport which is important to tumorigenesis. However, the mechanism of USO1 in colon cancer was still unknown. In our research, the expression of USO1 was knockdown in colon cancer cells (HCT116 and HT-29) by using a special lentivirus shRNA approach. A series of experiments were carried out to evaluate the effect of deregulation of USO1, including cell proliferation, apoptosis, cell migration and cell cycle. Knockdown of USO1 inhibits the ability of cell proliferation and migration. Furthermore, the deregulation of USO1 induces early apoptosis and decreased cells in G2-M phase. We demonstrate for the first time that USO1 gene has a critical role in human colon cancer. Our finding represents that USO1 gene may be a promising target for therapy and diagnosis in treatment of human colon cancer.
authors.affiliation: Department of Colorectal Surgery, Shanghai Changhai Hospital, 168 Changhai Road, Shanghai, 200433, People's Republic of China.
authors.forename: Jinke
authors.initials: J
authors.lastname: Sui
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Junjie
authors.initials: J
authors.lastname: Xing
authors.forename: Fuao
authors.initials: F
authors.lastname: Cao
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Gong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D000077614:Golgi Matrix Proteins; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000077614:Golgi Matrix Proteins; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115