title: The Daily Profiles of Circulating AMH and INSL3 in Men are Distinct from the Other Testicular Hormones, Inhibin B and Testosterone.
abstract: The testes secrete four hormones (anti-Müllerian hormone, insulin-like peptide 3, Inhibin B and testosterone) from two endocrine cell types. It is unknown whether anti-Müllerian hormone and insulin-like peptide 3 levels have a diurnal variation, and if so, whether they covary during the day with testosterone and InhB. Sera were obtained from 13 men at 00:00, 06:00, 09:00, 12:00, 14:00, 17:00 and 19:00 hours and the levels of their testicular hormones measured by ELISA. A second cohort of 20 men was similarly examined with blood drawn at 19:00 and the following 06:00. Anti-Müllerian hormone levels exhibited a subtle diurnal pattern with a 19:00 peak that was 4.9% higher on average than the 06:00 nadir (p = 0.004). The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B. Insulin-like peptide 3 had no diurnal pattern, with only minor sporadic variation between time points being observed in some men. In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type. Consequently, the balance of the hormones being released by the testes has complex variation during the day. The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.
authors.affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand; Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
authors.forename: Yih Harng
authors.initials: YH
authors.lastname: Chong
authors.affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Pankhurst
authors.affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand; Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
authors.forename: Ian S
authors.initials: IS
authors.lastname: McLennan
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D054304:Anti-Mullerian Hormone / Q000097:blood*; D002940:Circadian Rhythm; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007265:Inhibins / Q000097:blood*; D007328:Insulin / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011506:Proteins; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*; D055815:Young Adult
chemical_list: D007328:Insulin; C084797:Leydig insulin-like protein; D011506:Proteins; C422364:inhibin B; D013739:Testosterone; D007265:Inhibins; D054304:Anti-Mullerian Hormone