title: Diosgenin inhibits IL-1β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes.
abstract: It is well known that the inflammatory cytokines play important roles in osteoarthritis (OA). Diosgenin is a steroidal saponin found in several plants including Solanum and Dioscorea species and possesses diverse biological activities including anti-inflammatory properties. However, the role of diosgenin in inflammatory responses in OA chondrocytes is still unclear. Therefore, in this study, we investigated the anti-inflammatory properties of diosgenin in human OA chondrocytes. We found that diosgenin inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) induced by interleukin-1-beta (IL-1β). Diosgenin significantly inhibited the IL-1β-stimulated expression of metalloproteinase-3 (MMP-3), MMP-13, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in human OA chondrocytes. In addition, diosgenin suppressed the degradation of IκB-α in IL-1β-induced human OA chondrocytes. Taken together, this study showed that diosgenin can effectively inhibit the IL-1β-induced expression of inflammatory mediators, suggesting that diosgenin may be a potential agent in the treatment of OA.
authors.affiliation: Department of Orthopedics, Qilu Hospital, Shandong University Jinan 250000, Shandong, China ; Department of Orthopedics, Yantaishan Hospital Yantai 264000, Shandong, China.
authors.forename: Leisheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The People's Hospital of Qixia Qixia 265300, Shandong, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Qilu Hospital, Shandong University Jinan 250000, Shandong, China.
authors.forename: Yanpin
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, Yantaishan Hospital Yantai 264000, Shandong, China.
authors.forename: Shiqiao
authors.initials: S
authors.lastname: Lv
authors.affiliation: Department of Orthopedics, Yantaishan Hospital Yantai 264000, Shandong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Yantaishan Hospital Yantai 264000, Shandong, China.
authors.forename: Shaoxian
authors.initials: S
authors.lastname: Liu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004144:Diosgenin / Q000494:pharmacology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D053509:Matrix Metalloproteinase 13; D004144:Diosgenin; D015232:Dinoprostone
keywords: Diosgenin; chondrocytes; inflammation