title: MicroRNA-126 is down-regulated in human esophageal squamous cell carcinoma and inhibits the proliferation and migration in EC109 cell via PI3K/AKT signaling pathway.
abstract: MicroRNA-126 (miR-126) was found down-regulated in different types of cancer including esophageal squamous cell carcinoma (ESCC). However, the onco-genetic role of miR-126 in ESCC still remains unknown. In the present study, we found the relative expression of miR-126 in ESCC was significant decreased in ESCC tissues compared to adjacent normal tissues. Overexpression of miR-126 in EC109 cells resulted in significant decrease in cell proliferation, colon formation and migration. PI3K regulatory subunit p85 beta (PIK3R2), a member of PI3K/AKT signaling pathway was found upregulated in ESCC tissues and there is a negative relation between expression of PIK3R2 and miR-126. Restoration of miR-126 in EC109 cells induced a reduction in PIK3R2 protein levels, accompanied with a substantial reduction in phosphorylated AKT levels in EC109 cells, suggesting impairment in PI3K/AKT signaling pathway. The luciferase reporter assay confirmed that PIK3R2 was a direct target of miR-126. Furthermore, we also indicated overexpression of miR-126 suppresses G2/M transition in EC109 cells. Taken together, our study suggests that miR-126 functions as a potential tumor suppressor in ESCC progression via regulating PI3K/AKT signaling pathway partly by targeting PIK3R2, and targeting of miR-126 may provide a novel strategy for the diagnosis and treatment of ESCC.
authors.affiliation: Department of Clinical Laboratory, Shanghai Tenth People's Hospital Tongji University, Shanghai 200072, China.
authors.forename: Zheng-Chao
authors.initials: ZC
authors.lastname: Nie
authors.affiliation: Department of Clinical Laboratory, Shanghai Tenth People's Hospital Tongji University, Shanghai 200072, China.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Weng
authors.affiliation: Department of Clinical Laboratory, Chuzhou Second People's Hospital Chuzhou, China.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Shang
authors.affiliation: Department of Clinical Laboratory, Yangpu Hospital, Tongji University Shanghai 200090, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Long
authors.affiliation: Department of Clinical Laboratory, Shanghai Tenth People's Hospital Tongji University, Shanghai 200072, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Shanghai Tenth People's Hospital Tongji University, Shanghai 200072, China.
authors.forename: Yan-Ting
authors.initials: YT
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Yangpu Hospital, Tongji University Shanghai 200090, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D018450:Disease Progression; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: AKT; ESCC; PIK3R2; cell cycle arrest; miR-126