title: The Drosophila histone demethylase dKDM5/LID regulates hematopoietic development.
abstract: dKDM5/LID regulates transcription of essential developmental genes and, thus, is required for different developmental processes. Here, we report the essential contribution of dKDM5/LID to hematopoiesis in Drosophila. Our results show that dKDM5/LID is abundant in hemocytes and that its depletion induces over-proliferation and differentiation defects of larval hemocytes and disrupts organization of the actin cytoskeleton. We also show that dKDM5/LID regulates expression of key factors of hematopoietic development. In particular, dKDM5/LID depletion up-regulates expression of several transcription factors involved in hemocytes proliferation and differentiation as well as of several small-GTPases that link signaling effectors to actin cytoskeleton formation and dynamics.
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, Baldiri Reixac, 10, 08028 Barcelona, Spain. Electronic address: tomasmoraniturralde@gmail.com.
authors.forename: Tomás
authors.initials: T
authors.lastname: Morán
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, Baldiri Reixac, 10, 08028 Barcelona, Spain. Electronic address: jordi.bernues@ibmb.csic.es.
authors.forename: Jordi
authors.initials: J
authors.lastname: Bernués
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, Baldiri Reixac, 10, 08028 Barcelona, Spain. Electronic address: fambmc@ibmb.csic.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Azorín
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000201:enzymology*; D029721:Drosophila Proteins / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000235:genetics*; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D056466:Histone Demethylases / Q000502:physiology*; D007150:Immunohistochemistry; D007814:Larva / Q000201:enzymology; D008297:Male; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D029721:Drosophila Proteins; D014157:Transcription Factors; D056466:Histone Demethylases; C518655:Lid protein, Drosophila
keywords: Actin cytoskeleton; Drosophila hematopoiesis; Hemocytes differentiation; Hemocytes proliferation; dKDM5/LID