title: DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation.
abstract: To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.
authors.affiliation: Laboratory of Genome Research, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, DK-8000, Aarhus C, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Roedgaard
authors.affiliation: Laboratory of Genome Research, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, DK-8000, Aarhus C, Denmark.
authors.forename: Jacob
authors.initials: J
authors.lastname: Fredsoe
authors.affiliation: Laboratory of Genome Research, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, DK-8000, Aarhus C, Denmark.
authors.forename: Jakob Madsen
authors.initials: JM
authors.lastname: Pedersen
authors.affiliation: Laboratory of Genome Research, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, DK-8000, Aarhus C, Denmark.
authors.forename: Lotte
authors.initials: L
authors.lastname: Bjergbaek
authors.affiliation: Laboratory of Genome Research, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, DK-8000, Aarhus C, Denmark.
authors.forename: Anni Hangaard
authors.initials: AH
authors.lastname: Andersen
mesh_terms: D042002:Chromatin Assembly and Disassembly; D026942:DNA Topoisomerases / Q000378:metabolism*; D005686:Galactokinase / Q000235:genetics; D005690:Galactose / Q000378:metabolism*; D005800:Genes, Fungal; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: C518355:GAL10 protein, S cerevisiae; C106901:GAL2 protein, S cerevisiae; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase; D012319:RNA Polymerase II; D026942:DNA Topoisomerases; D005690:Galactose