title: Effects of venom immunotherapy on serum level of CCL5/RANTES in patients with Hymenoptera venom allergy.
abstract: Hymenoptera venoms are known to cause life-threatening IgE-mediated anaphylactic reactions in allergic individuals. Venom immunotherapy is a recommended treatment of insect allergy with still the mechanism not being completely understood. We decided to assess the serum CCL5/RANTES level in patients who experienced severe anaphylactic reaction to Hymenoptera venom and to find out changes in the course of immunotherapy. Twenty patients (9 men, 11 women, mean age: 31.91 ± 7.63 years) with history of anaphylactic reaction after insect sting were included into the study. Diagnosis was made according to sIgE and skin tests. All of them were enrolled into rush venom immunotherapy with bee or wasp venom extracts (Pharmalgen, ALK-Abello, Horsholm, Denmark). Serum levels of CCL5/RANTES were measured using a commercially available ELISA kit (R&D Systems, Minneapolis, MN). CCL5/RANTES serum concentration are higher in insect venom allergic patients than in healthy controls (887.5 ± 322.77 versus 387.27 ± 85.11 pg/ml). Serum concentration of CCL5/RANTES in insect venom allergic patient was significantly reduced in the course of allergen immunotherapy already after 6 days of vaccination (887.5 ± 322.77 versus 567.32 ± 92.16 pg/ml). CCL5/RANTES serum doesn't correlate with specific IgE. Chemokine CCL5/RANTES participates in allergic inflammation induced by Hymenoptera venom allergens. Specific immunotherapy reduces chemokine CCL5/RANTES serum level already after initial days of venom immunotherapy.
authors.affiliation: Chair and Clinical Department of Internal Diseases, Allergology and Clinical Immunology, Medical University of Silesia , Katowice , Poland.
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Gawlik
authors.forename: Joanna
authors.initials: J
authors.lastname: Glück
authors.forename: Barbara
authors.initials: B
authors.lastname: Jawor
authors.forename: Barbara
authors.initials: B
authors.lastname: Rogala
mesh_terms: D000328:Adult; D000707:Anaphylaxis / Q000097:blood / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage* / Q000276:immunology; D001180:Arthropod Venoms / Q000276:immunology*; D001514:Bee Venoms / Q000276:immunology; D018946:Chemokine CCL5 / Q000097:blood*; D003888:Desensitization, Immunologic / Q000379:methods*; D004334:Drug Administration Schedule; D005260:Female; D006801:Humans; D006927:Hymenoptera / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007299:Insect Bites and Stings / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012882:Skin Tests; D014862:Wasp Venoms / Q000276:immunology; D055815:Young Adult
chemical_list: D039741:Antigens, Dermatophagoides; D001180:Arthropod Venoms; D001514:Bee Venoms; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D014862:Wasp Venoms; D007073:Immunoglobulin E
keywords: Allergen immunotherapy; CCL5/RANTES; Hymenoptera venom allergy; IgE; chemokine