title: Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.
abstract: Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kurnik
authors.affiliation: Laboratory of Molecular Diagnostic and Biochemistry, Department of Potato Protection and Seed Science in Bonin, Plant Breeding and Acclimatization Institute-National Research Institute, 76-009 Bonin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Treder
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Skorupa-Kłaput
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland ; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tretyn
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland ; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Tyburski
keywords: Bioremediation; Peroxidase isoenzymes; Phenol; Potato pulp; Wastewater treatment