title: Transcriptome-facilitated development of SNPs for the Sonoran Desert rock fig, Ficus petiolaris (Moraceae).
abstract: OBJECTIVE
  Single-nucleotide polymorphism (SNP) primers were developed for a native North American desert fig, Ficus petiolaris (Moraceae), to provide markers for population genetic studies designed to quantify patterns of gene flow across a complex landscape.
  
  
  RESULTS
  Transcriptome sequencing and bioinformatic protocols were implemented to discover SNPs in single-copy protein-coding genes. Multiplexes of 30 nuclear and 24 organellar (chloroplast and mitochondrial) SNPs were selected for primer development and genotyping on the Sequenom MASSArray System. Of these 54 loci, 49 reliably amplified across a panel of 96 F. petiolaris individuals.
  
  
  CONCLUSIONS
  This study has provided SNP primers that can be applied in future studies investigating population genetics of F. petiolaris and its coevolution with associated pollinating and nonpollinating fig wasps.
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, 251 Bessey Hall, Ames, Iowa 50011 USA.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Davis
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, 251 Bessey Hall, Ames, Iowa 50011 USA.
authors.forename: Derek D
authors.initials: DD
authors.lastname: Houston
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, 251 Bessey Hall, Ames, Iowa 50011 USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Nason
keywords: Ficus petiolaris; Moraceae; RNA sequencing; population genomics; single nucleotide polymorphism; transcriptome sequencing