title: Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains.
abstract: Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features of M. colombiense strains remain unknown. In the present study, we demonstrated that M. colombiense strains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates. Thin-layer chromatography experiments showed that M. colombiense strains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) of M. avium. Conversely, we observed a natural rough variant of M. colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis in M. colombiense CECT 3035. RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis.
authors.affiliation: Chemistry Department, Faculty of Sciences, Universidad Nacional de Colombia, Carrera 30, No. 45-03, Ciudad Universitaria, Bogotá, Colombia.
authors.forename: Milena
authors.initials: M
authors.lastname: Maya-Hoyos
authors.affiliation: Chemistry Department, Faculty of Sciences, Universidad Nacional de Colombia, Carrera 30, No. 45-03, Ciudad Universitaria, Bogotá, Colombia.
authors.forename: John
authors.initials: J
authors.lastname: Leguizamón
authors.affiliation: Computational Biology Branch, NCBI, NLM, NIH, Bethesda, MD 20894-6075, USA ; Pan American Bioinformatics Institute, Santa Marta, Magdalena, Colombia.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Mariño-Ramírez
authors.affiliation: Chemistry Department, Faculty of Sciences, Universidad Nacional de Colombia, Carrera 30, No. 45-03, Ciudad Universitaria, Bogotá, Colombia.
authors.forename: Carlos Y
authors.initials: CY
authors.lastname: Soto
mesh_terms: D018441:Biofilms; D008563:Membrane Lipids / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D009161:Mycobacterium / Q000502:physiology*
chemical_list: D008563:Membrane Lipids