title: Evaluation of anti-inflammatory activity of the methanolic extract from Jungia rugosa leaves in rodents.
abstract: BACKGROUND
  Jungia rugosa Less (Asteraceae), popularly known in Ecuador as "Carne humana" or "Fompo", is a vine present into the Andean region. It is traditionally used as medicine for the treatment of bruises, cuts and other external inflammatory processes. This study was designed to investigate the anti-inflammatory activity of J. rugosa leaves extract (JRLE) in rodents.
  
  
  METHODS
  The acute anti-inflammatory activity was evaluated by animal models, including croton oil-induced ear oedema in mice, carrageenan-induced paw oedema in rats and myeloperoxidase (MPO); the chronic anti-inflammatory activity was evaluated by cotton pellet-induced granuloma.
  
  
  RESULTS
  Intraperitoneal administration of JRLE (125, 250, 500mg/kg) significantly (p<0.01-0.001) inhibited the croton oil-induced ear oedema and MPO activity in mice; the carrageenan-induced paw oedema in rats was significantly (p<0.05) reduced by 500mg/kg. Repeated (6 days) administration of the extract to mice previously implanted with cotton pellets reduced the formed granuloma (125mg/kg: 11.7%; 250mg/kg: 17.9%; 500mg/kg: 32.4%) but only the inhibition by 500mg/kg reached statistical significance (p<0.01).
  
  
  CONCLUSIONS
  The results show that JRLE is effective as an anti-inflammatory agent in acute and chronic inflammation in mice, supporting its traditional use.
authors.affiliation: Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador.
authors.forename: Isabel
authors.initials: I
authors.lastname: Wilches
authors.affiliation: Faculty of Engineering, University of Cuenca, Cuenca, Ecuador.
authors.forename: Vladimiro
authors.initials: V
authors.lastname: Tobar
authors.affiliation: Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Peñaherrera
authors.affiliation: Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador.
authors.forename: Nancy
authors.initials: N
authors.lastname: Cuzco
authors.affiliation: Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Jerves
authors.affiliation: Department Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussels-VUB, Belgium.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
authors.affiliation: Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador.
authors.forename: Fabián
authors.initials: F
authors.lastname: León-Tamariz
authors.affiliation: Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador; Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Electronic address: Elisabet.vila@ucuenca.edu.ec.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Vila
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D019659:Asteraceae; D002351:Carrageenan; D032561:Cotton Fiber; D003436:Croton Oil; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D015745:Granuloma, Foreign-Body / Q000188:drug therapy*; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012997:Solvents; D003436:Croton Oil; D002351:Carrageenan; D009195:Peroxidase; D000432:Methanol
keywords: Inflammation; Jungia rugosa; Mice; Pharmacodynamics; Traditional medicine Meso-Southern America