title: Prostate-specific RNA aptamer: promising nucleic acid antibody-like cancer detection.
abstract: We described the selection of a novel nucleic acid antibody-like prostate cancer (PCa) that specifically binds to the single-stranded DNA molecule from a 277-nt fragment that may have been partially paired and bound to the PCA3 RNA conformational structure. PCA3-277 aptamer ligands were obtained, and the best binding molecule, named CG3, was synthesized for validation. Aiming to prove its diagnostic utility, we used an apta-qPCR assay with CG3-aptamer conjugated to magnetic beads to capture PCA3 transcripts, which were amplified 97-fold and 7-fold higher than conventional qPCR in blood and tissue, respectively. Histopathologic analysis of 161 prostate biopsies arranged in a TMA and marked with biotin-labeled CG3-aptamer showed moderate staining in both cytoplasm and nucleus of PCa samples; in contrast, benign prostatic hyperplasia (BPH) samples presented strong nuclear staining (78% of the cases). No staining was observed in stromal cells. In addition, using an apta-qPCR, we demonstrated that CG3-aptamer specifically recognizes the conformational PCA3-277 molecule and at least three other transcript variants, indicating that long non-coding RNA (lncRNA) is processed after transcription. We suggest that CG3-aptamer may be a useful PCa diagnostic tool. In addition, this molecule may be used in drug design and drug delivery for PCa therapy.
authors.affiliation: 1] Laboratory of Cancer Molecular Genetics, Faculty of Medical Sciences, State University of Campinas, SP, Brazil [2] Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Marangoni
authors.affiliation: Laboratory of Molecular Biology, Institute of Biotechnology, Federal University of Goiás, Catalão/GO, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Neves
authors.affiliation: AC Camargo Cancer Hospital, SP, Brazil.
authors.forename: Rafael M
authors.initials: RM
authors.lastname: Rocha
authors.affiliation: Laboratory of Histology, Institute of Biomedical Sciences, Federal University of Uberlândia, MG, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Faria
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Patrícia T
authors.initials: PT
authors.lastname: Alves
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Aline G
authors.initials: AG
authors.lastname: Souza
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Patrícia T
authors.initials: PT
authors.lastname: Fujimura
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Fabiana A A
authors.initials: FA
authors.lastname: Santos
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil.
authors.forename: Thaise G
authors.initials: TG
authors.lastname: Araújo
authors.affiliation: Laboratory of Cancer Molecular Genetics, Faculty of Medical Sciences, State University of Campinas, SP, Brazil.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Ward
authors.affiliation: 1] Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, MG, Brazil [2] University of California-Davis, Dept. of Medical Microbiology and Immunology, Davis/CA, USA.
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Goulart
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood / Q000235:genetics*; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000097:blood / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009690:Nucleic Acid Conformation; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D046888:Tissue Array Analysis
chemical_list: D000974:Antibodies, Antinuclear; D000951:Antigens, Neoplasm; D052157:Aptamers, Nucleotide; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D062085:RNA, Long Noncoding; C479341:prostate cancer antigen 3, human; D012313:RNA