title: High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique.
abstract: Cryostat sectioning is a popular but labor-intensive method for preparing histological brain sections. We have developed a modification of the commercially available CryoJane tape collection method that significantly improves the ease of collection and the final quality of the tissue sections. The key modification involves an array of UVLEDs to achieve uniform polymerization of the glass slide and robust adhesion between the section and slide. This report presents system components and detailed procedural steps, and provides examples of end results; that is, 20 μm mouse brain sections that have been successfully processed for routine Nissl, myelin staining, DAB histochemistry, and fluorescence. The method is also suitable for larger brains, such as rat and monkey.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America; Department of Biomedical Engineering at Stony Brook University, Stony Brook, New York, United States of America.
authors.forename: Vadim
authors.initials: V
authors.lastname: Pinskiy
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America.
authors.forename: Jamie
authors.initials: J
authors.lastname: Jones
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Tolpygo
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America.
authors.forename: Neil
authors.initials: N
authors.lastname: Franciotti
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America.
authors.forename: Kevin
authors.initials: K
authors.lastname: Weber
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America.
authors.forename: Partha P
authors.initials: PP
authors.lastname: Mitra
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000737:chemistry; D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D016616:Cryoultramicrotomy / Q000295:instrumentation / Q000379:methods*; D000882:Haplorhini; D057166:High-Throughput Screening Assays; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D009186:Myelin Sheath / Q000648:ultrastructure; D009562:Nissl Bodies / Q000648:ultrastructure; D051381:Rats; D020318:Rats, Long-Evans; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods*
chemical_list: D015100:3,3'-Diaminobenzidine