title: Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury.
abstract: Dendrimers and dendriplexes, highly branched synthetic macromolecules, have gained popularity as new tools for a variety of nanomedicine strategies due to their unique structure and properties. We show that fluorescent phosphorus dendrimers are well retained by bone marrow-derived macrophages and exhibit robust spectral shift in its emission in response to polarization conditions. Fluorescence properties of this marker can also assist in identifying macrophage presence and phenotype status at different time points after spinal cord injury. Potential use of a single dendrimer compound as a drug/siRNA carrier and phenotype-specific cell tracer offers new avenues for enhanced cell therapies combined with monitoring of cell fate and function in spinal cord injury.
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada. shakhbazau@gmail.com.
authors.forename: Antos
authors.initials: A
authors.lastname: Shakhbazau
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Manoj
authors.initials: M
authors.lastname: Mishra
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Tak-Ho
authors.initials: TH
authors.lastname: Chu
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Craig
authors.initials: C
authors.lastname: Brideau
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Karen
authors.initials: K
authors.lastname: Cummins
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Tsutsui
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.affiliation: Laboratoire de Chimie de Coordination, CNRS, Toulouse, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Majoral
authors.affiliation: Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologique, Université Paris Descartes, Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mignani
authors.affiliation: Institut des Sciences Moléculaires, Université Bordeaux, Talence cedex, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Blanchard-Desce
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: V Wee
authors.initials: VW
authors.lastname: Yong
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Peter K
authors.initials: PK
authors.lastname: Stys
authors.affiliation: Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada.
authors.forename: Jan
authors.initials: J
authors.lastname: van Minnen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D050091:Dendrimers / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D051379:Mice; D050997:Nanomedicine / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology
chemical_list: D050091:Dendrimers
keywords: cell tracker; fluorescent phosphorus dendrimer; macrophage polarization; spectral shift; spinal cord injury