title: Coupling chemical oxidation and biostimulation: Effects on the natural attenuation capacity and resilience of the native microbial community in alkylbenzene-polluted soil.
abstract: Coupling chemical oxidation with bioremediation could be a cost-effective system to cope with soil and groundwater pollution. However, the effects of chemical oxidation on autochthonous microbial communities are scarcely known. A detailed analysis that considers both the efficiency of the two technologies and the response of the microbial communities was performed on a linear alkylbenzene-polluted soil and groundwater samples. The impacts of a modified Fenton's reaction (MFR) at various dosages and of permanganate on the microbiota over 4 weeks were assessed. The permanganate and MFR negatively affected microbial abundance and activity. However, the resilience of certain microbial populations was observed, with a final increase in potential hydrocarbon-degrading populations as determined by both the alkB gene abundance and the predominance of well-known hydrocarbon-degrading phylotypes such as Rhodococcus, Ochrobactrum, Acinetobacter and Cupriavidus genera as determined by 16S rRNA-based DGGE fingerprinting. The assessment of the chemical oxidant impact on autochthonous microbiota should be considered for the optimization of coupled field remediation technologies.
authors.affiliation: Department of Microbiology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
authors.forename: Eulàlia
authors.initials: E
authors.lastname: Martínez-Pascual
authors.affiliation: Department of Environmental Technology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Tim
authors.initials: T
authors.lastname: Grotenhuis
authors.affiliation: Department of Microbiology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Solanas
authors.affiliation: GIRO Joint Research Unit IRTA-UPC, IRTA, Torre Marimon, E-08140 Caldes de Montbui, Spain. Electronic address: marc.vinas@irta.cat.
authors.forename: Marc
authors.initials: M
authors.lastname: Viñas
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001555:Benzene Derivatives / Q000737:chemistry* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011196:Potassium Permanganate / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001555:Benzene Derivatives; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D011196:Potassium Permanganate; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D007501:Iron; D010100:Oxygen
keywords: Alkylbenzenes (LAB); Biostimulation; Chemical oxidation; Microbial resilience; Modified Fenton; Permanganate