title: Biochemical indicators and biomarkers in chub (Squalius cephalus L.) from the Sava River.
abstract: Biochemical indicators and biomarkers were analyzed in the liver and gills of chub caught in three localities along the Sava River exposed to different environmental impacts. Sampling sites were: downstream from Zagreb (Zgd), downstream Sremska Mitrovica (SM) and downstream from Belgrade (Bgd). We observed that the relative amounts and levels of activity of Cu, Zn containing superoxide dismutase and glutathione in both the liver and gills, and the relative amounts of heat shock protein (HSP90) and metallothioneins in the gills were highest in the Zgd locality, suggesting a higher impact of metal pollution. The Zgd locality had higher concentrations of trace metals in the water, especially iron. In the SM and Bgd localities, higher relative levels of glutathione peroxidase and catalase were recorded (especially in SM) as compared to the Zgd locality, pointing to the presence of hydrogen peroxide and different classes of organic peroxides. Low water oxygen and high temperature levels in the Bgd locality suggesting different metabolic activity between examined locations. Our results suggest that different presence and concentrations of individual environmental factors (total environment) influence the way how fish establish homeostasis.
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Mihailović
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: dblagoje@ibiss.bg.ac.rs.
authors.forename: Duško
authors.initials: D
authors.lastname: Blagojević
authors.affiliation: Department of Environmental Sciences, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
authors.forename: Nives
authors.initials: N
authors.lastname: Ogrinc
authors.affiliation: Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
authors.forename: Predrag
authors.initials: P
authors.lastname: Simonović
authors.affiliation: Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Vladica
authors.initials: V
authors.lastname: Simić
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Melita
authors.initials: M
authors.lastname: Vidaković
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Dinić
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Uskoković
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Nevena
authors.initials: N
authors.lastname: Grdović
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Arambašić-Jovanović
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Miloš
authors.initials: M
authors.lastname: Đorđević
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Anja
authors.initials: A
authors.lastname: Tolić
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Margareta
authors.initials: M
authors.lastname: Kračun-Kolarević
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Stoimir
authors.initials: S
authors.lastname: Kolarević
authors.affiliation: University of Zagreb, Faculty of Agriculture, Department of Fisheries, Beekeeping, Game Management and Spec. Zoology, Svetosimunska 25, 10000 Zagreb, Croatia.
authors.forename: Marina
authors.initials: M
authors.lastname: Piria
authors.affiliation: Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Momir
authors.initials: M
authors.lastname: Paunović
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003530:Cyprinidae / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D013030:Spain; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D005978:Glutathione
keywords: Antioxidant enzymes; Biomarkers; CYP4501A; Heat shock protein; Metallothioneins; Sava River