title: Epigenetic harnessing of HCV via modulating the lipid droplet-protein, TIP47, in HCV cell models.
abstract: This study aimed at identifying potential microRNAs that modulate hepatic lipid droplets (LD) through targeting the Tail interacting protein of 47 kDa (TIP47) in HCV infection. Bioinformatics analysis revealed that miR-148a and miR-30a potentially target TIP47. Expression profiling showed that both microRNAs were downregulated, while TIP47 was upregulated in liver biopsies of HCV-infected patients. Forcing the expression of both microRNAs in JFH-I infected, oleic acid-treated Huh7 cells, significantly suppressed TIP47 expression and reduced cellular LDs with marked decrease in viral RNA. This study shows that miR-148a and miR-30a, regulate TIP47 expression and LDs in HCV infected cells.
authors.affiliation: Molecular Pathology Research Group, Department of Pharmacology and Toxicology, German University in Cairo, Cairo, Egypt.
authors.forename: Nada M
authors.initials: NM
authors.lastname: El-Ekiaby
authors.affiliation: Molecular Pathology Research Group, Department of Pharmacology and Toxicology, German University in Cairo, Cairo, Egypt.
authors.forename: Radwa Y
authors.initials: RY
authors.lastname: Mekky
authors.affiliation: Molecular Pathology Research Group, Department of Pharmaceutical Biology, German University in Cairo, Cairo, Egypt.
authors.forename: Shereen A
authors.initials: SA
authors.lastname: El Sobky
authors.affiliation: Molecular Pathology Research Group, Department of Pharmacology and Toxicology, German University in Cairo, Cairo, Egypt.
authors.forename: Noha M
authors.initials: NM
authors.lastname: Elemam
authors.affiliation: Endemic Medicine and Hepatology, Cairo University, Cairo, Egypt.
authors.forename: Mohammed
authors.initials: M
authors.lastname: El-Sayed
authors.affiliation: Endemic Medicine and Hepatology, Cairo University, Cairo, Egypt.
authors.forename: Gamal
authors.initials: G
authors.lastname: Esmat
authors.affiliation: Molecular Pathology Research Group, Department of Pharmacology and Toxicology, German University in Cairo, Cairo, Egypt. Electronic address: ahmed.abdel-aziz@guc.edu.eg.
authors.forename: Ahmed I
authors.initials: AI
authors.lastname: Abdelaziz
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000368:Aged; D001483:Base Sequence; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D000070796:Perilipin-3; D034622:RNA Interference; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D033921:Vesicular Transport Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C550570:MIRN148 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C038196:PLIN3 protein, human; D000070796:Perilipin-3; D012367:RNA, Viral; D033921:Vesicular Transport Proteins
keywords: HCV; Lipid droplets; Tail interacting protein of 47kDa; miR-148a; miR-30a