title: Characterization and immobilization of Trametes versicolor laccase on magnetic chitosan-clay composite beads for phenol removal.
abstract: Laccase from Trametes versicolor was immobilized on magnetic chitosan-clay composite beads by glutaraldehyde crosslinking. The physical, chemical, and biochemical properties of the immobilized laccase and its application in phenol removal were comprehensively investigated. The structure and morphology of the composite beads were characterized by SEM, TGA, and FTIR analyses. The immobilized laccase showed better storage stability and higher tolerance to the changes in pH and temperature compared with free laccase. Moreover, the immobilized laccase retained more than 75% of its original activity after 10 cycles. The efficiency of phenol removal by immobilized laccase was about 80% under the optimum conditions after 4 h.
authors.affiliation: a Celal Bayar University, Science and Arts Faculty, Chemistry Department , Manisa , Turkey.
authors.forename: Tülin
authors.initials: T
authors.lastname: Aydemir
authors.affiliation: a Celal Bayar University, Science and Arts Faculty, Chemistry Department , Manisa , Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Güler
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D000077215:Clay; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000302:isolation & purification; D017743:Equipment Reuse; D005618:Fresh Water / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D059346:Magnets; D008863:Microspheres; D010636:Phenols / Q000302:isolation & purification*; D013696:Temperature; D055454:Trametes / Q000737:chemistry / Q000201:enzymology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods
chemical_list: D000538:Aluminum Silicates; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D010636:Phenols; D014874:Water Pollutants, Chemical; D048271:Chitosan; D042845:Laccase; D000077215:Clay; D005976:Glutaral
keywords: Trametes versicolor laccase; chitosan; enzyme immobilization; magnetic; phenol removal