title: [Association of irradiation-induced hippocampal neurogenesis impairment with histone H3 acetylation].
abstract: OBJECTIVE
  To explore the effect of whole brain irradiation (WBI) on neurogenesis in hippocampus and its relationship with histone acetylation.
  
  
  METHODS
  A signal dose of 30Gy 4MeV electron beam was offered to male Sprague-Dawley rats (150 - 200 g). At Days 7 and 30, immunohistochemistry and Western blot were used to analyze the effects of WBI on neurogenesis and the expression of acetyl-H3 and histone deacetylase 1 (HDAC1) in hippocampus. Finally trichostatin A (TSA), a HDAC1 inhibitor, was used to verify whether H3 acetylation was associated with neurogenesis impairment.
  
  
  RESULTS
  Immunofluorescence showed that, at Day 7 post-irradiation, the number of BrdU + NeuN + cells reduced by 67% (P < 0. 01) and it approached zero at Day 30 (P < 0. 01). Meanwhile, a significant decrease of HDAC1-dependent H3 acetylation was observed. Western blot showed 30% (P <0. 05) and 61% (P < 0. 01) reductions in H3 acetylation at Days 7 and 30 post-irradiation respectively. These results were further confirmed by immunofluorescent staining. Also HDAC1 levels significantly increased in a time-dependent manner at Days 7 and 30 post-irradiation. And TSA rescued neurogenesis impairment after WBI.
  
  
  CONCLUSIONS
  Radiation-induced HDAC1-dependent H3 acetylation decline is associated with long-term neurogenesis impairment in dentate gyrus.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Ji
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.forename: Shang
authors.initials: S
authors.lastname: Cai
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Ji
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lyuan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000107:Acetylation; D000818:Animals; D001930:Brain Injuries; D006624:Hippocampus; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D008297:Male; D055495:Neurogenesis; D011833:Radiation Injuries, Experimental; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A