title: Quantitative Fitness Analysis Identifies exo1∆ and Other Suppressors or Enhancers of Telomere Defects in Schizosaccharomyces pombe.
abstract: Synthetic genetic array (SGA) has been successfully used to identify genetic interactions in S. cerevisiae and S. pombe. In S. pombe, SGA methods use either cycloheximide (C) or heat shock (HS) to select double mutants before measuring colony size as a surrogate for fitness. Quantitative Fitness Analysis (QFA) is a different method for determining fitness of microbial strains. In QFA, liquid cultures are spotted onto solid agar and growth curves determined for each spot by photography and model fitting. Here, we compared the two S. pombe SGA methods and found that the HS method was more reproducible for us. We also developed a QFA procedure for S. pombe. We used QFA to identify genetic interactions affecting two temperature sensitive, telomere associated query mutations (taz1Δ and pot1-1). We identify exo1∆ and other gene deletions as suppressors or enhancers of S. pombe telomere defects. Our study identifies known and novel gene deletions affecting the fitness of strains with telomere defects. The interactions we identify may be relevant in human cells.
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Narayanan
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: Marion
authors.initials: M
authors.lastname: Dubarry
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: Conor
authors.initials: C
authors.lastname: Lawless
authors.affiliation: High Throughput Screening Facility, Newcastle Biomedicine, Newcastle University, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: A Peter
authors.initials: AP
authors.lastname: Banks
authors.affiliation: School of Mathematics & Statistics, Newcastle University, Newcastle upon Tyne, NE1 7RU, United Kingdom.
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Wilkinson
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: Simon K
authors.initials: SK
authors.lastname: Whitehall
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University Medical School, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Lydall
mesh_terms: D004742:Enhancer Elements, Genetic / Q000502:physiology; D017353:Gene Deletion; D016153:Genes, Suppressor / Q000502:physiology; D056084:Genetic Fitness / Q000502:physiology*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D016615:Telomere / Q000235:genetics* / Q000502:physiology