title: Site-directed spin labeling of 2'-amino groups in RNA with isoindoline nitroxides that are resistant to reduction.
abstract: Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2'-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by pulsed EPR. After conjugation to RNA, a tetraethyl isoindoline derivative showed significant stability under reducing conditions.
authors.affiliation: University of Iceland, Department of Chemistry, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland. snorrisi@hi.is.
authors.forename: Subham
authors.initials: S
authors.lastname: Saha
authors.forename: Anil P
authors.initials: AP
authors.lastname: Jagtap
authors.forename: Snorri Th
authors.initials: ST
authors.lastname: Sigurdsson
mesh_terms: D001665:Binding Sites; D054833:Isoindoles / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D012313:RNA / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D054833:Isoindoles; D013113:Spin Labels; D012313:RNA