title: A New Turn on Fluorescent Probe for Selective Detection of Cysteine/Homocysteine.
abstract: A new fluorescent probe (probe 1) was developed for recognition of cysteine (Cys) and homocysteine (Hcy). Probe 1 exhibited a large absorption peak blue-shift (107 nm) as well as enhanced fluorescence responses to Cys/Hcy based on cyclization of thiol containing amino acids with aldehydes, inhibiting the C = N isomerization-induced quenching process by an intramolecular hydrogen bond. The detection mechanism was proved by (1)H NMR and mass spectrometry analysis.
authors.affiliation: College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. phxie2013@163.com.
authors.forename: Puhui
authors.initials: P
authors.lastname: Xie
authors.affiliation: College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Guangqin
authors.initials: G
authors.lastname: Gao
authors.affiliation: College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Jin
authors.affiliation: College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Yang
mesh_terms: D003545:Cysteine / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006710:Homocysteine / Q000032:analysis* / Q000737:chemistry*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D006710:Homocysteine; D003545:Cysteine
keywords: Cys/Hcy; Fluorescent probe; Inhibiting C = N isomerization; Intramolecular hydrogen bond; Ratiometric probes