title: Elimination of allogeneic multipotent stromal cells by host macrophages in different models of regeneration.
abstract: Allogeneic multipotent stromal cells were previously thought to be poorly recognized by host immune system; the prolonged survival in host environments was explained by their immune privileged status. As long as the concept is currently reconsidered, the routes of elimination of allogeneic multipotent stromal cells by host immunity must be taken into account. This is necessary for correct comprehension of their therapeutic action. The study was focused upon survival of umbilical cord-derived allogeneic multipotent stromal cells in different rat models of tissue regeneration induced by partial hepatectomy or by critical limb ischemia. The observations were carried out by means of vital labeling of the cells with PKH26 prior to injection, in combination with differential immunostaining of host macrophages with anti-CD68 antibody. According to the results, allogeneic multipotent stromal cells are specifically eliminated by host immune system; the efficacy can reach 100%. Massive clearance of transplanted cells by host macrophages is accompanied by appropriation of the label by the latter, and this is a pronounced case of misleading presentation of exogenous label by host cells. The study emphasizes the role of macrophages in host response and also the need of additional criteria for correct data interpretation.
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia ; Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Arutyunyan
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia ; Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia ; Pirogov Russian National Research Medical University, Ministry of Healthcare of The Russian Federation 1 Ostrovitianov Street, Moscow 117997, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Elchaninov
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia ; Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia ; Pirogov Russian National Research Medical University, Ministry of Healthcare of The Russian Federation 1 Ostrovitianov Street, Moscow 117997, Russia.
authors.forename: Timur
authors.initials: T
authors.lastname: Fatkhudinov
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia ; Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia ; Pirogov Russian National Research Medical University, Ministry of Healthcare of The Russian Federation 1 Ostrovitianov Street, Moscow 117997, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Makarov
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia ; Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia.
authors.forename: Evgeniya
authors.initials: E
authors.lastname: Kananykhina
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia ; Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia ; Pirogov Russian National Research Medical University, Ministry of Healthcare of The Russian Federation 1 Ostrovitianov Street, Moscow 117997, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Usman
authors.affiliation: Scientific Research Institute of Human Morphology 3 Tsurupa Street, Moscow 117418, Russia.
authors.forename: Galina
authors.initials: G
authors.lastname: Bolshakova
authors.affiliation: Pirogov Russian National Research Medical University, Ministry of Healthcare of The Russian Federation 1 Ostrovitianov Street, Moscow 117997, Russia.
authors.forename: Valeria
authors.initials: V
authors.lastname: Glinkina
authors.affiliation: Research Centre of Medical Genetics 1 Moskvorechie Street, Moscow 115478, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Goldshtein
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of The Russian Federation 4 Oparina Street, Moscow 117997, Russia.
authors.forename: Gennady
authors.initials: G
authors.lastname: Sukhikh
mesh_terms: D064591:Allografts; D000818:Animals; D002470:Cell Survival / Q000276:immunology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006498:Hepatectomy; D007150:Immunohistochemistry; D008115:Liver Regeneration / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*
keywords: Allogeneic transplantation; CD68 antigen; macrophages; mesenchymal stromal cells; multipotent stromal cells