title: Antimicrobial, Antioxidant, Anti-Inflammatory, and Cytotoxic Activities of Propolis from the Stingless Bee Tetragonisca fiebrigi (Jataí).
abstract: Propolis from stingless bees Tetragonisca fiebrigi found in Brazil is used in folk medicine by their nutritional and therapeutic properties. However, there are no scientific records evidencing such properties. The present study was designed to investigate the chemical composition and the biological properties of propolis from T. fiebrigi. For this, the chemical composition of the ethanol extract of propolis (EEP) was determined by GC-MS and presented phenolic compounds, alcohol, and terpenes as its major class compounds. The antimicrobial activity was accessed in gram-positive and gram-negative bacteria and in fungi, isolated from different biological fluids and reference strains. The EEP was active against all microorganisms and showed antioxidant activity by scavenging free radicals, inhibiting hemolysis and lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The anti-inflammatory potential of the EEP was confirmed by inhibition of the hyaluronidase enzyme. The cytotoxic activity was concentration-dependent against K562 cells, with a predominance of death by necrosis. Taken together, these results show that propolis from T. fiebrigi has important therapeutic activities, which suggest its potential application in the pharmaceutical industry, as well as in health foods, beverages, and nutritional supplements.
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Jaqueline Ferreira
authors.initials: JF
authors.lastname: Campos
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Uilson Pereira
authors.initials: UP
authors.lastname: Dos Santos
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Paola Dos Santos
authors.initials: Pdos S
authors.lastname: da Rocha
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Marcio José
authors.initials: MJ
authors.lastname: Damião
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: José Benedito Perrella
authors.initials: JB
authors.lastname: Balestieri
authors.affiliation: Course of Chemistry, State University of Mato Grosso do Sul, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Claudia Andrea Lima
authors.initials: CA
authors.lastname: Cardoso
authors.affiliation: Department of Biochemistry, Federal University of São Paulo, Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil.
authors.forename: Edgar Julian
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.affiliation: CIMO/ESA, Department of Biology and Biotechnology, Agricultural College of Bragança, Polytechnic Institute of Bragança, Campus Santa Apolónia, 5301-855 Bragança, Portugal.
authors.forename: Leticia Miranda
authors.initials: LM
authors.lastname: Estevinho
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Kely
authors.initials: K
authors.lastname: de Picoli Souza
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Edson Lucas
authors.initials: EL
authors.lastname: Dos Santos