title: A novel titania/calcium silicate hydrate hierarchical coating on titanium.
abstract: Recently, surface micron/nano-topographical modifications have attracted a great deal of attention because it is capable of mimicking the hierarchical characteristics of bone. In the current work, a novel titania/calcium silicate hydrate (CSH) bi-layer coating with hierarchical surface topography was successfully prepared on titanium substrate through micro-arc oxidation (MAO) and subsequent hydrothermal treatment (HT). MAO treatment could lead to a micron-scale topographical surface with numerous crater-like protuberances. The subsequent HT process enables the in situ nucleation and growth of CSH nanoplates on MAO-fabricated titania surface. The nucleation of CSH nanoplates is considered to follow a dissolution-precipitation mechanism. Compared to MAO-fabricated coating with single-scale surface topography, MAO-HT-fabricated coating with hierarchical surface topography exhibits enhanced hydrophilicity, fibronectin adsorption and initial MG-63 cell attachment. The process of cell-material interactions is considered to be triggered by surface properties of the coated layer and indirectly mediated by protein adsorption on coating surface. These results suggest that MAO-HT treatment is an efficient way to prepare coatings with hierarchical surface topography on titanium surface, which is essential for altering protein adsorption and initial cell attachment.
authors.affiliation: State key laboratory of new ceramics and fine processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State key laboratory of new ceramics and fine processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Xujie
authors.initials: X
authors.lastname: Liu
authors.affiliation: State key laboratory of new ceramics and fine processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; Biomaterials Department, Inorganic Chemical Industries Division, National Research Centre, Dokki, 12622 Cairo, Egypt.
authors.forename: Tarek A
authors.initials: TA
authors.lastname: Elkhooly
authors.affiliation: State key laboratory of new ceramics and fine processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State key laboratory of new ceramics and fine processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Shen
authors.affiliation: State key laboratory of new ceramics and fine processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. Electronic address: biomater@mail.tsinghua.edu.cn.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
mesh_terms: D000327:Adsorption; D017610:Calcium Compounds / Q000737:chemistry*; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000737:chemistry; D017640:Silicates / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D011506:Proteins; D017640:Silicates; D014025:Titanium; C031293:calcium silicate
keywords: Hierarchical surface topography; Hydrothermal treatment; Micro-arc oxidation; Titanium implant