title: S-genotype identification based on allele-specific PCR in Japanese pear.
abstract: Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific PCR primer pairs for ten previously reported S-RNase alleles (S (1)-S (9) and S (k)) as simple and reliable method. Specific nucleotide sequences were chosen to design the primers to amplify fragments of only the corresponding S alleles. The developed primer pairs were evaluated by using homozygous S-genotypes (S (1)/S (1)-S (9)/S (9) and S (4sm)/S (4sm)) and 14 major Japanese pear cultivars, and found that S allele-specific primer pairs can identify S-genotypes effectively. The S allele-specific primer pairs developed in this study will be useful for efficient S-genotyping and for marker-assisted selection in Japanese pear breeding programs.
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Nashima
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Terakami
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Sogo
authors.initials: S
authors.lastname: Nishio
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kunihisa
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Nishitani
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Saito
authors.affiliation: NARO Institute of Fruit Tree Science , 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605 , Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Yamamoto
keywords: Pyrus pyrifolia; S-RNase; S-genotyping; self-incompatibility