title: Bone marrow-derived mesenchymal stem cell transplantation ameliorates oxidative stress and restores intestinal mucosal permeability in chemically induced colitis in mice.
abstract: BACKGROUND
  Ulcerative colitis (UC) can be viewed as an autoimmune disease. Bone marrow-derived mesenchymal stem cells (MSCs) with its regenerative, cellular multi-lineage and immunomodulatory abilities can influence the repair of damaged tissues in UC. This study investigated the effects of MSCs transplantation on the mice intestinal barrier in response to oxidative stress injury.
  
  
  METHODS
  Colitis was induced by daily consecutive administration of 5% dextran sulfate sodium (DSS) solution for 7 days. Male murine MSCs were isolated and transplanted into female mice via injection in the tail vein. Serum and colon specimens were collected at 12 h, 24 h, 3 d, 7 d and 14 d after injection. Serum levels of D-lactate (D-LAC), diamine oxidase (DAO), colonic levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were quantified. The SRY protein of the male sex determinant gene expression and E-cadherin were also ascertained intracellularly.
  
  
  RESULTS
  Three days after receiving male MSCs transplantation, SRY protein expression was detected. The quantity increased on successive days. Serum levels of D-LAC and DAO, colonic MDA and SOD normalized in a shorter time period compared to controls (p<0.05). Not surprisingly, histological regeneration of tissue and E-cadherin expression in the colon of MSCs transplanted mice also occurred in a shorter time period than controls.
  
  
  CONCLUSIONS
  Transplanted MSCs restored mucosal permeability, and minimized oxidative stress related injury.
authors.affiliation: Naval General Hospital Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Naval General Hospital Beijing, China.
authors.forename: Guang-Zhou
authors.initials: GZ
authors.lastname: Gao
authors.affiliation: Naval General Hospital Beijing, China.
authors.forename: Rong-Fu
authors.initials: RF
authors.lastname: Li
authors.affiliation: Naval General Hospital Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Naval General Hospital Beijing, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.affiliation: Naval General Hospital Beijing, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wu
authors.affiliation: Jersey City, NJ, USA.
authors.forename: Anthony Et
authors.initials: AE
authors.lastname: Yeo
authors.affiliation: Department of Gastroenterology, The 309th Hospital of People's Liberation Army China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
keywords: Mesenchymal stem cell; cell transplantation; intestinal barrier; mice; ulcerative colitis