title: Phylogenetic study of metallo-β-lactamase producing multidrug resistant Pseudomonas aeruginosa isolates from burn patients.
abstract: The present study was carried out to understand the clonal relationship using enterobacteriaceae repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) among metallo-β-lactamase (MBL) producing multidrug resistant Pseudomonas aeruginosa isolates from burn victims and their susceptibility to commonly used anti-pseudomonal agents. In the present study 94 non-duplicate P. aeruginosa strains from the wound samples of burn patients were included. Identification of the isolates was done by biochemical methods and antibiotic sensitivity was done by disc diffusion method following CLSI (Clinical Laboratory Standard Institute) guidelines. By using imipenem (IPM)-EDTA disk diffusion/double disc synergy method carbapenem resistant organisms were tested for MBL. To define the clonal relationship ERIC-PCR was used. Of the 94 isolates, 18 (19.14%) were found resistant to IPM and MBL production was shown 11 (11.70%) by the IPM-EDTA disc diffusion method. From dendrogram of the ERIC-PCR profile four major clusters were obtained (A, B, C and D). Cluster B contained the majority of the isolates (6 strains 1, 4, 8, 9, 10 and 11). This study using ERIC-PCR of randomly collected isolates exhibits high genetic diversity which rules out cross contamination frequency.
authors.affiliation: Department of Microbiology, IMS & SUM Hospital, Siksha 'O' Anusandhan University, Khandagiri, Bhubaneswar, Odisha 751003, India. Electronic address: jayanti.mits06@gmail.com.
authors.forename: Jayanti
authors.initials: J
authors.lastname: Jena
authors.affiliation: Department of Microbiology, IMS & SUM Hospital, Siksha 'O' Anusandhan University, Khandagiri, Bhubaneswar, Odisha 751003, India. Electronic address: Dr.debata@gmail.com.
authors.forename: Nagen Kumar
authors.initials: NK
authors.lastname: Debata
authors.affiliation: Centre of Biotechnology, Siksha 'O' Anusandhan University, Khandagiri, Bhubaneswar, Odisha 751003, India. Electronic address: rajeshkumarsahoo@soauniversity.ac.in.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Sahoo
authors.affiliation: Centre of Biotechnology, Siksha 'O' Anusandhan University, Khandagiri, Bhubaneswar, Odisha 751003, India. Electronic address: enketeswarasubudhi@soauniversity.ac.in.
authors.forename: Enketeswara
authors.initials: E
authors.lastname: Subudhi
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002056:Burns / Q000382:microbiology*; D002442:Ceftazidime / Q000494:pharmacology; D002443:Ceftriaxone / Q000494:pharmacology; D015377:Cilastatin / Q000494:pharmacology; D019818:Clavulanic Acid / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D004338:Drug Combinations; D024901:Drug Resistance, Multiple, Bacterial; D014644:Genetic Variation; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008911:Minocycline / Q000031:analogs & derivatives / Q000494:pharmacology; D009428:Netilmicin / Q000494:pharmacology; D015242:Ofloxacin / Q000494:pharmacology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D000078304:Tigecycline; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D015377:Cilastatin; D019818:Clavulanic Acid; D009428:Netilmicin; D000078304:Tigecycline; D015378:Imipenem; D002443:Ceftriaxone; D000583:Amikacin; D002442:Ceftazidime; D015242:Ofloxacin; D001618:beta-Lactamases; D008911:Minocycline; D003091:Colistin
keywords: Burn; ERIC-PCR; Metallo-β-lactamase (MBL); Pseudomonas aeruginosa