title: Evaluation of the potential anti-allergic effects of heat-inactivated Lactobacillus paracasei V0151 in vitro, ex vivo, and in vivo.
abstract: The efficacy of Lactobacillus paracasei V0151 (V0151), isolated from the faeces of a child, to modulate immune responses was investigated. In RAW 264.7 cells expressing an inducible nitric oxide synthase (iNOS)-directed luciferase gene, heat-inactivated V0151 stimulated iNOS expression followed by nitric oxide production. V0151 significantly elevated interferon gamma, interleukin (IL)-10, tumour necrosis factor alpha, and IL-1β production in human peripheral blood mononuclear cells. In splenocytes isolated from ovalbumin (OVA)-sensitised BALB/c mice treated with OVA and V0151 at different bacterium-to-cell ratios (1:1, 10:1, and 20:1) for 96 h, IL-2, IL-4, IL-5, and IL-13 production was dose-dependently downregulated, whereas IL-12 was dose-dependently upregulated. Collectively, our findings indicate that V0151 might regulate pro-inflammatory factors in macrophages and splenocytes. Furthermore, the T helper 1/T helper 2 (Th1/Th2) balance was also skewed toward Th1 dominance through the elevation of Th1 cytokine production.
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.
authors.forename: Y W
authors.initials: YW
authors.lastname: Liu
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.|2 Probiotics Research Center, National Yang-Ming University, No. 155, Li-Nong St., Section 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.
authors.forename: T Y
authors.initials: TY
authors.lastname: Fu
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.|2 Probiotics Research Center, National Yang-Ming University, No. 155, Li-Nong St., Section 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.
authors.forename: W S
authors.initials: WS
authors.lastname: Peng
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.|2 Probiotics Research Center, National Yang-Ming University, No. 155, Li-Nong St., Section 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.
authors.forename: Y H
authors.initials: YH
authors.lastname: Chen
authors.affiliation: 3 Want Want China Holdings Ltd., No.1088, East Hond Song Rd., Shanghai 201103, China P.R.
authors.forename: Y M
authors.initials: YM
authors.lastname: Cao
authors.affiliation: 3 Want Want China Holdings Ltd., No.1088, East Hond Song Rd., Shanghai 201103, China P.R.
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.affiliation: 3 Want Want China Holdings Ltd., No.1088, East Hond Song Rd., Shanghai 201103, China P.R.
authors.forename: W L
authors.initials: WL
authors.lastname: Hung
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.|2 Probiotics Research Center, National Yang-Ming University, No. 155, Li-Nong St., Section 2, BeiTou Dist., Taipei 11221, Taiwan, R.O.C.
authors.forename: Y C
authors.initials: YC
authors.lastname: Tsai
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D006358:Hot Temperature; D007778:Lactobacillus / Q000276:immunology* / Q000528:radiation effects*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II
keywords: Lactobacillus paracasei; immune modulation; macrophages; ovalbumin; splenocytes