title: Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co-Cultured With Mature Neuronal Cells.
abstract: For electrical stimulation of hMSCs, gold nanoparticles were coated onto polyethyleneimine coated glass cover slips. The effects of pulsed or constant electrical stimulation upon cytotoxicity and differentiation of hMSCs were examined. The effects of co culturing hMSCs with neuronal cells were also tested. The neuronal differentiation of the stem cells was evaluated by determining the expression of neuron-speciﬁc genes and proteins using RT-PCR and Western blotting. Morphological changes were evaluated by scanning electron microscopy. The hMSCs co-cultured with mature neuronal cells and stimulated with electrical shock showed the greatest level of neurite outgrowth (>150 mm) and smaller cell body sizes.
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul-si, 139-240, Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-Complex, 689 Sampyeong-dong Bundang-gu, Seongnam-si, 463-400, Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-Complex, 689 Sampyeong-dong Bundang-gu, Seongnam-si, 463-400, Korea.
authors.forename: Han Na
authors.initials: HN
authors.lastname: Yang
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-Complex, 689 Sampyeong-dong Bundang-gu, Seongnam-si, 463-400, Korea.
authors.forename: Se Won
authors.initials: SW
authors.lastname: Yi
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul-si, 139-240, Korea. chkim@kirams.re.kr.
authors.forename: Chun-Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-Complex, 689 Sampyeong-dong Bundang-gu, Seongnam-si, 463-400, Korea. pkh0410@cha.ac.kr.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
mesh_terms: D018920:Coculture Techniques; D004558:Electric Stimulation; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D016501:Neurites / Q000378:metabolism* / Q000648:ultrastructure; D055495:Neurogenesis
keywords: differentiation; electrical stimulation; gold nanoparticles; hMSCs; nerve cell