title: Gene-gene interactions among CX3CL1, LEPR and IL-6 related to coronary artery disease in Chinese Han population.
abstract: OBJECTIVE
  The purpose of this study was to investigate the impact of the interactions among CX3CL1 (rs170364 and rs614230), LEPR (rs6700896), and IL-6 (rs2066992) polymorphisms on the risk of coronary artery disease (CAD) in Chinese Han population.
  
  
  METHODS
  120 CAD patients and 109 healthy controls were enrolled in the study. Polymerase chain reaction (PCR) and direct sequencing methods were used to analyze the genotypes of CX3CL1, LEPR, and IL-6 polymorphisms. Multifactor dimensionality reduction (MDR) software was utilized to analyze gene-gene interactions. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were used for evaluating the association between gene polymorphisms or gene-gene interactions and CAD risk.
  
  
  RESULTS
  In the study, TT genotype of rs170364 in CX3CL1 might decrease the CAD risk (OR=0.39, 95% CI=0.16-0.98). No significant correlation was found between T allele of rs170364 and CAD risk (P>0.05). CC genotype and C allele in rs614230 (CX3CL1) were significantly related with decreased risk of CAD (OR=0.38, 95% CI=0.17-0.86; OR=0.66, 95% CI=0.45-0.97). For IL-6 rs2066992 polymorphism. GG genotype could increase the risk of CAD (OR=2.32, 95% CI=1.04-5.17). Whereas, no significant correlation was observed between LEPR rs6700896 and CAD susceptibility. MDR analysis showed that CX3CL1, LEPR and IL-6 genes might jointly promote the occurrence of CAD.
  
  
  CONCLUSIONS
  The interactions of CX3CL1, LEPR and IL-6 genes might increase the risk of CAD.
authors.affiliation: Department of Intensive Care Unit, The First Clinical Hospital Affliated to Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Song-Gen
authors.initials: SG
authors.lastname: Jin
authors.affiliation: The First Ward of Department of Infection, The First Clinical Hospital Affliated to Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Chen
authors.affiliation: Department of Intensive Care Unit, The First Clinical Hospital Affliated to Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Song-Liu
authors.initials: SL
authors.lastname: Yang
authors.affiliation: Department of Intensive Care Unit, The First Clinical Hospital Affliated to Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D054428:Chemokine CX3CL1 / Q000235:genetics*; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D003324:Coronary Artery Disease / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D054411:Receptors, Leptin / Q000235:genetics*; D012307:Risk Factors
chemical_list: C515538:CX3CL1 protein, human; D054428:Chemokine CX3CL1; C508600:IL6 protein, human; D015850:Interleukin-6; C515448:LEPR protein, human; D054411:Receptors, Leptin
keywords: CX3CL1; IL-6; LEPR; coronary artery disease; interaction