title: The preliminary study on the effects of growth hormone and insulin-like growth factor-I on κ-casein synthesis in bovine mammary epithelial cells in vitro.
abstract: The effects of growth hormone (GH) and insulin-like growth factor-I (IGF-I) on protein synthesis and gene expression of κ-casein in bovine mammary epithelial cell in vitro were studied. The treatments were designed as follows: the growth medium without serum was set as the control group, while the treatments were medium supplemented with GH (100 ng/ml), IGF-I (100 ng/ml), and GH (100 ng/ml) + IGF-I (100 ng/ml). The quantity of κ-casein protein was measured by ELISA, and the κ-casein gene (CSN3) expression was examined by real-time quantitative PCR (RT-qPCR). Compared with the control group, all the experimental groups had greater (p < 0.05) expression of CSN3. The concentration of κ-casein followed a similar response as CSN3, but the difference between the treatments and the control was not statistically significant (p > 0.05). Furthermore, no synergistic effect of GH and IGF-I was observed for both the κ-casein concentration and CSN3 expression. It is therefore concluded that GH or IGF-I can independently promote the expression of CSN3 in bovine mammary epithelial cells in vitro.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ji
authors.affiliation: School of Clinical Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: L M
authors.initials: LM
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: H R
authors.initials: HR
authors.lastname: Wang
authors.affiliation: Department of Animal Sciences and Division of Nutritional Science, University of Illinois, Urbana, IL, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Loor
mesh_terms: D000818:Animals; D002364:Caseins / Q000096:biosynthesis*; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000166:cytology*
chemical_list: D002364:Caseins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone
keywords: CSN3 gene expression; growth hormone; insulin-like growth factor-I; κ-casein protein