title: C35 is overexpressed in colorectal cancer and is associated tumor invasion and metastasis.
abstract: The aim of this study was to investigate the expression of C35, an oncogene previously found in breast and prostate cancers, and its clinicopathological significance in colorectal cancer (CRC). Qualitative and quantitative detection of C35 mRNA expression was performed using reverse transcription-PCR (RT-PCR) and real-time PCR. C35 protein expression was determined using immunohistochemistry. C35 mRNA was detected in none of 10 normal colorectal tissue samples, 55 of 65 (84.6%) CRC tissue samples, and 43 of 55 (78.2%) adjacent non-cancerous tissue samples. In addition, the level of C35 mRNA in CRC tissue samples was markedly higher than that in tumor adjacent non-cancerous tissue samples. C35 protein expression was detected in 58 of 80 (72.5%) CRC tissue samples and was closely associated with tumor serosal invasion, lymphnode metastasis, and an advanced Dukes stage. These results suggest that C35 might serve as a biomarker or therapeutic target for management of CRC.
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Lingbin
authors.initials: L
authors.lastname: Kong
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Jingxuan
authors.initials: J
authors.lastname: Kou
authors.forename: Lixin
authors.initials: L
authors.lastname: Yin
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C517054:MIEN1 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger