title: Correlation between Vascular Endothelial Growth Factor C Expressior and Lymph Node Micrometastasis and Prognosis in Patients with pNO Esophageal Squamous Cell Carcinomas.
abstract: OBJECTIVE
  The purpose of this study was to investigate the correlation between vascular endothelial growth factor C expression and lymph node micrometastasis and prognosis in patients with pN0 esophageal squamous cell carcinoma.
  
  
  METHODS
  A total of 528 lymph nodes obtained from the 87 patients with pN0 esophageal squamous cell carcinoma who underwent Ivor-Lewis esophagectomy were reevaluated by reverse transcriptasepolymerase chain reaction to detect mucin 1 mRNA. Vascular endothelial growth factor C mRNA was detected in esophageal cancer issues also by re-evaluated by reverse transcriptasepolymerase chain reaction.
  
  
  RESULTS
  Vascular endothelial growth factor C mRNA expression was correlated with tumor invasion (P < 0.01) and lymphatic invasion (P = 0.000). In univariate analysis by log-rank test, the 5-year survival rate in patients after operation was significantly associated with tumor invasion (P = 0.026), mucin 1 mRNA expression (P = 0.000) and vascular endothelial growth factor C mRNA expression (P = 0.020). The results of Cox regression multivariate analysis confirmed that tumor invasion status and mucin 1 mRNA expression were the independent relevant factors.
  
  
  CONCLUSIONS
  Expression of vascular endothelial growth factor C is related to tumor invasion and lymphatic invasion and is not related to lymph node micrometastasis in patients with pN0 esophageal squamous cell carcinoma.
authors.forename: Zlhi-Gang
authors.initials: ZG
authors.lastname: Sun
authors.forename: Xiang-Yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000556:secondary* / Q000601:surgery; D016009:Chi-Square Distribution; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D000077277:Esophageal Squamous Cell Carcinoma; D016629:Esophagectomy; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000235:genetics; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D061206:Neoplasm Micrometastasis; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C060192:MUC1 protein, human; D018396:Mucin-1; D012333:RNA, Messenger; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C