title: Identification of differentially expressed proteins of normal and cancerous human colorectal tissues by liquid chromatograph-mass spectrometer based on iTRAQ approach.
abstract: Liquid chromatograph-mass spectrometer (LC/MS) based labeled with isobaric mass tags for relative and absolute quantitation (iTRAQ) analyses were performed to identify differentially expressed proteins from normal and cancerous human colorectal tissues. Around 802 proteins were identified, 68 proteins of which were defined as differentially expressed proteins. Bioinformatics analysis indicated that these differentially expressed proteins correlated with several specific cellular processes and pathways which have relationships with pathological changes of colorectal cancer (CRC). EHD2 were selected to verify its expression patterns and localization using western blotting and immunohistochemistry respectively. LC/MS-based iTRAQ proteomic approach would provide new information about the character of CRC.
authors.affiliation: a 1 Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army , Luoyang, China .|b 2 The Second Military Medical University , Shanghai, China .
authors.forename: Jingjing
authors.initials: J
authors.lastname: Lv
authors.affiliation: a 1 Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army , Luoyang, China .
authors.forename: Naijun
authors.initials: N
authors.lastname: Fan
authors.affiliation: c 3 Department of Pathology, No. 150 Central Hospital of Chinese People's Liberation Army , Luoyang, China.
authors.forename: Yangkun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a 1 Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army , Luoyang, China .
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wang
authors.affiliation: a 1 Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army , Luoyang, China .
authors.forename: Chunfang
authors.initials: C
authors.lastname: Gao
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D002853:Chromatography, Liquid / Q000379:methods; D015179:Colorectal Neoplasms / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009363:Neoplasm Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; C406446:EHD2 protein, human; D009363:Neoplasm Proteins
keywords: Cancer; Colorectal Tissues; Differentially expressed proteins; LC/MS; iTRAQ approach