title: Using Vitek MALDI-TOF mass spectrometry to identify species belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex: a relevant alternative to molecular biology?
abstract: Acinetobacter baumannii belongs to the Acinetobacter calcoaceticus-baumannii complex (Acb) containing 2 other pathogenic species: Acinetobacter pittii and Acinetobacter nosocomialis. Identification of these bacteria remains problematic despite the use of matrix-assisted laser ionization time-of-flight mass spectrometry (MALDI-TOF MS). Here, we enriched the SARAMIS™ database of the Vitek MS® plus mass spectrometer to improve the identification of species of the Acb complex. For each species, we incremented reference spectra. Then, a SuperSpectrum was created based on the selection of 40 specific masses. In a second step, we validated reference spectra and SuperSpectra with 100 isolates identified by rpoB gene sequencing. All the isolates were correctly identified by MALDI-TOF MS with the database we created as compared to the identifications obtained by rpoB sequencing. Our database enabled rapid and reliable identification of the pathogen species belonging to the Acb complex. Identification by MALDI-TOF MS with our database is a good alternative to molecular biology.
authors.affiliation: Laboratoire de Bactériologie, Institut de Biologie en Santé-PBH, CHU, 4 rue Larrey, 49933 Angers cedex, France; ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Pailhoriès
authors.affiliation: Laboratoire de Bactériologie, Institut de Biologie en Santé-PBH, CHU, 4 rue Larrey, 49933 Angers cedex, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Daure
authors.affiliation: Laboratoire de Bactériologie, Institut de Biologie en Santé-PBH, CHU, 4 rue Larrey, 49933 Angers cedex, France; ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Eveillard
authors.affiliation: Laboratoire de Bactériologie, Institut de Biologie en Santé-PBH, CHU, 4 rue Larrey, 49933 Angers cedex, France; ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Joly-Guillou
authors.affiliation: Laboratoire de Bactériologie, Institut de Biologie en Santé-PBH, CHU, 4 rue Larrey, 49933 Angers cedex, France; ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France. Electronic address: makempf@chu-angers.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Kempf
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D016954:Acinetobacter calcoaceticus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
keywords: Acinetobacter baumannii; Acinetobacter pittii; MALDI-TOF mass spectrometry; SARAMIS™; SuperSpectrum; Vitek MS® plus