title: Evaluation of intravenous regional perfusion with amphotericin B and dimethylsulfoxide to treat horses for pythiosis of a limb.
abstract: BACKGROUND
  Treatment for horses with pythiosis of a limb is challenging. This study aims to evaluate the effects of administering amphotericin B in a 10 % solution of dimethylsulfoxide by intravenous regional limb perfusion (IRLP) to treat horses for cutaneous pythiosis of a limb.
  
  
  RESULTS
  All 15 of the horses treated had complete resolutions of their lesion between 6 to 9 weeks after a single IRLP treatment. No complications were observed at the site of venipuncture for IRLP. Before initiation of treatment, there was anemia and marked leucocytosis which resolved following treatment. Serum biochemistry showed no significant changes.
  
  
  CONCLUSIONS
  IRLP administration of amphotericin B in a 10 % DMSO solution was easily performed, relatively inexpensive and an effective treatment for treating horses for pythiosis of a limb and resolved the infection with no complications.
authors.affiliation: Department of Veterinary Medicine, University of São Paulo, Duque de Caxias Norte ave 225, Pirassununga, ZIP 13635-900, SP, Brazil. redoria@usp.br.
authors.forename: Renata G S
authors.initials: RG
authors.lastname: Dória
authors.affiliation: Department of Veterinary Medicine, University of Cuiabá, Cuiabá, MT, Brazil. mama_buenocarvalho@hotmial.com.
authors.forename: Mariana B
authors.initials: MB
authors.lastname: Carvalho
authors.affiliation: Department of Veterinary Medicine, University of São Paulo, Duque de Caxias Norte ave 225, Pirassununga, ZIP 13635-900, SP, Brazil. shfreitas@terra.com.br.
authors.forename: Silvio H
authors.initials: SH
authors.lastname: Freitas
authors.affiliation: Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil. luciane-laskoski@hotmail.com.
authors.forename: Luciane M
authors.initials: LM
authors.lastname: Laskoski
authors.affiliation: Department of Veterinary Pathology, Federal University of Mato Grosso, Cuiabá, MT, Brazil. moleta@gmail.com.
authors.forename: Edson M
authors.initials: EM
authors.lastname: Colodel
authors.affiliation: Department of Morphology and Physiology, Federal Rural University of Pernambuco, Recife, PE, Brazil. mendoncafs@yahoo.com.br.
authors.forename: Fábio S
authors.initials: FS
authors.lastname: Mendonça
authors.affiliation: Department of Veterinary Medicine, Federal University of Tocantins, Araguaína, TO, Brazil. silva_vet@hotmail.com.
authors.forename: Marco A G
authors.initials: MA
authors.lastname: Silva
authors.affiliation: Department of Veterinary Medicine, University of São Paulo, Duque de Caxias Norte ave 225, Pirassununga, ZIP 13635-900, SP, Brazil. renangrigoletto@gmail.com.
authors.forename: Renan
authors.initials: R
authors.lastname: Grigoletto
authors.affiliation: Department of Veterinary Medicine, University of São Paulo, Duque de Caxias Norte ave 225, Pirassununga, ZIP 13635-900, SP, Brazil. fantinatont@gmail.com.
authors.forename: Paulo
authors.initials: P
authors.lastname: Fantinato Neto
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005260:Female; D005552:Forelimb / Q000382:microbiology / Q000473:pathology; D006614:Hindlimb / Q000382:microbiology / Q000473:pathology; D006734:Horse Diseases / Q000188:drug therapy*; D006736:Horses; D007275:Injections, Intravenous; D008297:Male; D058968:Pythiosis / Q000188:drug therapy*; D011775:Pythium / Q000302:isolation & purification*
chemical_list: D000890:Anti-Infective Agents; D000666:Amphotericin B; D004121:Dimethyl Sulfoxide