title: [Expression of SDF-1/CXCR4 in acute infarct myocardium after implantation of bone marrow mesenchymal stem cells in rats].
abstract: OBJECTIVE
  To explore the role of stromal cell derived factor-1 (SDF-1)/CXCR4 axis in acute infarct myocardium after an implantation of bone marrow mesenchymal stem cells (MSCs) in rats.
  
  
  METHODS
  The animals were anesthetized by an intraperitoneal injection of pentobarbital sodium. Left anterior thoracotomy through 3/4 intercostals region was performed and then left anterior descending coronary arteries were ligated for modeling acute myocardial infarction. The MSCs were injected into the area of acute infarct myocardium after a 10-minute ligation of left anterior descending coronary artery. The different concentration and expression of SDF-1/CXCR4 in the area of acute myocardial infarction and left ventricular function were analyzed.
  
  
  RESULTS
  At day 28 post-transplantation, the vascular density in MSCs implant group were significantly higher than that in control group (P<0.05). And left ventricular function in MSCs implant group improved significantly than that in control group too (P<0.05). At the same time, the expressions of SDF-1 and CXCR4 in topical injection sites of infarct myocardium were significantly higher than those in control group (P<0.05). In MSCs implant group, the level of SDF-1/CXCR4 peaked at Day 1 post-transplantation and then it declined.
  
  
  CONCLUSIONS
  After the implantation of MSCs into acute infarct myocardium, there is vascular regeneration and the level of SDF-1/CXCR4 increases so that left ventricular function improves. And the mechanism may be due to an up-regulation of SDF-1/CXCR4 axis.
authors.affiliation: Department of Cardiology, Affiliated Hospital, Zunyi Medical College, Zunyi 563000, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
authors.forename: Guanxue
authors.initials: G
authors.lastname: Xu
authors.forename: Ranzun
authors.initials: R
authors.lastname: Zhao
authors.forename: Changyin
authors.initials: C
authors.lastname: Shen
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Email: shibei2147@163.com.
authors.forename: Bei
authors.initials: B
authors.lastname: Shi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009203:Myocardial Infarction; D009206:Myocardium; D051381:Rats; D019718:Receptors, CXCR4; D012038:Regeneration; D015398:Signal Transduction; D015854:Up-Regulation; D016277:Ventricular Function, Left
chemical_list: C486264:Cxcr4 protein, rat; D019718:Receptors, CXCR4