title: Decrease of oxidative phosphorylation system function in severe septic patients.
abstract: OBJECTIVE
  The comparison of oxidative phosphorylation system capacities between septic patients and control subjects has been scarcely analyzed and only in studies with small sample size (fewer than 40 septic patients and 40 controls). Thus, the objective of this study was to compare platelet respiratory complex IV (CIV) activity between severe septic patients and healthy individuals in a larger series (including 198 severe septic patients and 96 healthy controls).
  
  
  METHODS
  A prospective, multicenter, observational study was carried out in 6 Spanish intensive care units. We obtained blood samples from 198 severe septic patients at day 1, 4, and 8 of the severe sepsis diagnosis and 96 sex- and age-matched healthy control individuals and determined platelet CIV-specific activity. The end point of the study was 30-day mortality.
  
  
  RESULTS
  Control individuals showed higher platelet CIV-specific activity (P < .001) than surviving (n = 130) or nonsurviving (n = 68) severe septic patients at day 1, 4, and 8 of severe sepsis diagnosis.
  
  
  CONCLUSIONS
  The major finding of our work, involving the largest series to date of severe septic patients with data on oxidative phosphorylation system capacity, was that surviving and nonsurviving septic patients showed lower platelet CIV-specific activity during the first week of sepsis than healthy controls.
authors.affiliation: Intensive Care Unit, Hospital Universitario de Canarias, Ofra, s/n La Laguna, 38320, Santa Cruz de Tenerife, Spain. Electronic address: lorentemartin@msn.com.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lorente
authors.affiliation: Intensive Care Unit, Hospital Universitario Nuestra Señora Candelaria, Crta Rosario s/n Santa Cruz Tenerife, 38010, Spain. Electronic address: mar.martinvelasco@gmail.com.
authors.forename: María M
authors.initials: MM
authors.lastname: Martín
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Centro de Investigaciones Biomédicas En Red de Enfermedades Raras e Instituto de Investigación Sanitaria de Aragón, Universidad de Zaragoza, Zaragoza, 50013, Spain. Electronic address: esterlop@unizar.es.
authors.forename: Ester
authors.initials: E
authors.lastname: López-Gallardo
authors.affiliation: Intensive Care Unit, Hospital Clínico Universitario de Valencia, Avda Blasco Ibáñez n°17-19, Valencia, 46004, Spain. Electronic address: blanquer_jos@gva.es.
authors.forename: José
authors.initials: J
authors.lastname: Blanquer
authors.affiliation: Intensive Care Unit, Hospital Universitario Dr Negrín, Barranco de la Ballena s/n Las Palmas de Gran Canaria, 35010, Spain. Electronic address: jsolvio@gobiernodecanarias.org.
authors.forename: Jordi
authors.initials: J
authors.lastname: Solé-Violán
authors.affiliation: Intensive Care Unit, Hospital San Jorge de Huesca, Avenida Martínez de Velasco n°36 Huesca, 22004, Spain. Electronic address: llabarta@salud.aragon.es.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Labarta
authors.affiliation: Intensive Care Unit, Hospital Insular, Plaza Dr Pasteur s/n Las Palmas de Gran Canaria, 35016, Spain. Electronic address: incaicos@yahoo.es.
authors.forename: César
authors.initials: C
authors.lastname: Díaz
authors.affiliation: Research Unit, Hospital Universitario de Canarias, Ofra, s/n La Laguna, 38320, Santa Cruz de Tenerife, Spain. Electronic address: ajimenezsosa@gmail.com.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Jiménez
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Centro de Investigaciones Biomédicas En Red de Enfermedades Raras e Instituto de Investigación Sanitaria de Aragón, Universidad de Zaragoza, Zaragoza, 50013, Spain. Electronic address: jmontoya@unizar.es.
authors.forename: Julio
authors.initials: J
authors.lastname: Montoya
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Centro de Investigaciones Biomédicas En Red de Enfermedades Raras e Instituto de Investigación Sanitaria de Aragón, Universidad de Zaragoza, Zaragoza, 50013, Spain. Electronic address: eduruiz@unizar.es.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ruiz-Pesini
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D005260:Female; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000378:metabolism / Q000401:mortality; D010085:Oxidative Phosphorylation; D011446:Prospective Studies; D018805:Sepsis / Q000378:metabolism* / Q000401:mortality
chemical_list: D015415:Biomarkers
keywords: Cytochrome c oxidase; Mitochondria; Mortality; OXPHOS; Sepsis