title: Methods for enrichment of novel electrochemically-active microorganisms.
abstract: Electrochemically-active microorganisms (EAM) are relevant to metal biogeochemistry and have applications in microbial fuel cells (MFCs), bioremediation, and bioelectrocatalysis. Most research conducted to date focuses on EAM hailing from two distinct genera, namely Shewanella and Geobacter, with a relatively limited number of EAM discovered in recent years. This review article summarises current approaches to novel EAM enrichment, in terms of inoculum choice, growth medium, reactor configuration, electrochemical characterisation and community profiling through metagenomics and metatranscriptomics. A novel roadmap for EAM enrichment and subsequent characterisation using environmental samples as a starting material is provided in order to increase throughput and hence the likelihood of discovering novel EAM.
authors.affiliation: Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 60 Nanyang Drive, SBS-01N-27, Singapore 637551, Singapore; Interdisciplinary Graduate School, Nanyang Technological University, Singapore.
authors.forename: Lucinda Elizabeth
authors.initials: LE
authors.lastname: Doyle
authors.affiliation: Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 60 Nanyang Drive, SBS-01N-27, Singapore 637551, Singapore; School of Biotechnology, Dublin City University, Collins Avenue, Dublin, Ireland. Electronic address: emarsili@ntu.edu.sg.
authors.forename: Enrico
authors.initials: E
authors.lastname: Marsili
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media; D004563:Electrochemistry / Q000379:methods*; D062065:Wastewater / Q000382:microbiology
chemical_list: D003470:Culture Media; D062065:Wastewater
keywords: Electrochemically-active bacteria; Enrichment; Microbial fuel cells; Sediments