title: Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly.
abstract: In many cell types, septins assemble into filaments and rings at the neck of cellular appendages and/or at the cleavage furrow to help compartmentalize the plasma membrane and support cytokinesis. How septin ring assembly is coordinated with membrane remodeling and controlled by mechanical stress at these sites is unclear. Through a genetic screen, we uncovered an unanticipated link between the conserved Rho1 GTPase and its effector protein kinase C (Pkc1) with septin ring stability in yeast. Both Rho1 and Pkc1 stabilize the septin ring, at least partly through phosphorylation of the membrane-associated F-BAR protein Syp1, which colocalizes asymmetrically with the septin ring at the bud neck. Syp1 is displaced from the bud neck upon Pkc1-dependent phosphorylation at two serines, thereby affecting the rigidity of the new-forming septin ring. We propose that Rho1 and Pkc1 coordinate septin ring assembly with membrane and cell wall remodeling partly by controlling Syp1 residence at the bud neck.
authors.affiliation: Centre de Recherche en Biochimie Macromoléculaire, 34293 Montpellier, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Merlini
authors.affiliation: Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Alessio
authors.initials: A
authors.lastname: Bolognesi
authors.affiliation: Centre de Recherche en Biochimie Macromoléculaire, 34293 Montpellier, France.
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Juanes
authors.affiliation: Functional Proteomic Platform, Institut de Génomique Fonctionnelle, 34094 Montpellier, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Vandermoere
authors.affiliation: Centre de Recherche en Biochimie Macromoléculaire, 34293 Montpellier, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Courtellemont
authors.affiliation: Centre de Recherche en Biochimie Macromoléculaire, 34293 Montpellier, France.
authors.forename: Roberta
authors.initials: R
authors.lastname: Pascolutti
authors.affiliation: Functional Proteomic Platform, Institut de Génomique Fonctionnelle, 34094 Montpellier, France.
authors.forename: Martial
authors.initials: M
authors.lastname: Séveno
authors.affiliation: Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Yves
authors.initials: Y
authors.lastname: Barral
authors.affiliation: Centre de Recherche en Biochimie Macromoléculaire, 34293 Montpellier, France simonetta.piatti@crbm.cnrs.fr.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Piatti
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D048749:Cytokinesis / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D058112:Septins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; C546997:Syp1 protein, S cerevisiae; C490702:PKC1 protein, S cerevisiae; D011493:Protein Kinase C; D058112:Septins; C051302:RHO1 protein, S cerevisiae; D020741:rho GTP-Binding Proteins