title: Kix domain specific Immunoglobulin A can protect from adverse lung and cerebral pathology induced by Plasmodium berghei ANKA.
abstract: Plasmodium specific IgA has been detected in serum and breast milk among the endemic population but the role it can play in vivo is not clear. In this report, we demonstrate the utility of Malaria specific IgA, elicited by peptide sequences (referred as Mpep3 and Mpep4) of region VI of EBA-175 (PfrVI). Immunization of mice with KLH tagged or untagged peptides of Mpep3, Mpep4 or with PfrVI have resulted in specific IgA response that inhibits the in vitro invasion of Plasmodium falciparum merozoites. Mice having the IgA specific to Mpep4 have exhibited higher tolerance to Plasmodium berghei ANKA parasitemia, exhibited several fold lesser sequestration of infected RBC, lesser damage to microvasculature with no signs of perivascular haemorrhage and lesser lung inflammation in comparison to unimmunized mice. In addition, the immunized mice have B-cell population that secrete the IgA specific to PfrVI. These results suggest that the IgA specific to these malarial antigens can confer significant advantage to hosts and it may also reduce the severity of malaria infection.
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India.
authors.forename: Sapna
authors.initials: S
authors.lastname: Deore
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India.
authors.forename: Tanuja
authors.initials: T
authors.lastname: Bankar
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Lotke
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Parab
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune, 411007, India. Electronic address: mvks@nccs.res.in.
authors.forename: Musti
authors.initials: M
authors.lastname: Krishnasastry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D001921:Brain / Q000276:immunology / Q000469:parasitology* / Q000473:pathology; D005260:Female; D007106:Immune Sera; D007114:Immunization; D007070:Immunoglobulin A / Q000737:chemistry / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000469:parasitology; D008288:Malaria / Q000276:immunology / Q000401:mortality / Q000469:parasitology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010962:Plasmodium berghei / Q000276:immunology / Q000472:pathogenicity*; D010963:Plasmodium falciparum / Q000276:immunology / Q000472:pathogenicity; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D007106:Immune Sera; D007070:Immunoglobulin A; D010446:Peptide Fragments; D015800:Protozoan Proteins; C065668:erythrocyte-binding antigen 175, Plasmodium
keywords: Cerebral malaria; Erythrocyte binding antigen-175; Immunoglobulin A; Kix domain; Lung inflammation; Plasmodium berghei ANKA; Plasmodium falciparum; Region VI