title: Adenylylsulfate-ammonia adenylyltransferase activity is another inherent property of Fhit proteins.
abstract: Fhits (fragile histidine triad proteins) occur in eukaryotes but their function is largely unknown, although human Fhit is believed to act as a tumour suppressor. Fhits also exhibit dinucleoside triphosphatase, adenylylsulfatase and nucleoside phosphoramidase activities that in each case yield nucleoside 5'-monophosphate as a product. Due to the dinucleoside triphosphatase activity, Fhits may also be involved in mRNA decapping. In the present study, we demonstrate Fhit-catalysed ammonolysis of adenosine 5'-phosphosulfate, which results in the formation of adenosine 5'-phosphoramidate. This reaction has previously been associated with adenylylsulfate-ammonia adenylyltransferase (EC 2.7.7.51). Our finding shows that the capacity to catalyse ammonolysis is another inherent property of Fhits. Basic kinetic parameters and substrate specificity of this reaction catalysed by human Fhit are presented.
authors.affiliation: Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632 Poznań, Poland.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Wojdyła-Mamoń
authors.affiliation: Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632 Poznań, Poland guranow@up.poznan.pl.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Guranowski
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry* / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D031297:Lupinus / Q000201:enzymology* / Q000235:genetics; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D029681:Arabidopsis Proteins; D009363:Neoplasm Proteins; D009713:Nucleotidyltransferases; D015800:Protozoan Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C033080:adenylyl sulfate-ammonia adenylyltransferase
keywords: adenosine 5′-phosphoramidate; adenosine 5′-phosphorofluoridate; adenosine 5′-phosphosulfate; fragile histidine triad (Fhit) proteins; fragile histidine triad protein (Fhit)-catalysed ammonolysis