title: An advanced fragment analysis-based individualized subtype classification of pediatric acute lymphoblastic leukemia.
abstract: Pediatric acute lymphoblastic leukemia (ALL) is the most common neoplasm and one of the primary causes of death in children. Its treatment is highly dependent on the correct classification of subtype. Previously, we developed a microarray-based subtype classifier based on the relative expression levels of 62 marker genes, which can predict 7 different ALL subtypes with an accuracy as high as 97% in completely independent samples. Because the classifier is based on gene expression rank values rather than actual values, the classifier enables an individualized diagnosis, without the need to reference the background distribution of the marker genes in a large number of other samples, and also enables cross platform application. Here, we demonstrate that the classifier can be extended from a microarray-based technology to a multiplex qPCR-based technology using the same set of marker genes as the advanced fragment analysis (AFA). Compared to microarray assays, the new assay system makes the convenient, low cost and individualized subtype diagnosis of pediatric ALL a reality and is clinically applicable, particularly in developing countries.
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1] CAS Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences. Shanghai, China [2] Graduate School of Chinese Academy of Sciences, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Ningbo Health Gene Technologies Ltd. Ningbo, Zhejiang, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Ningbo Health Gene Technologies Ltd. Ningbo, Zhejiang, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Ningbo Health Gene Technologies Ltd. Ningbo, Zhejiang, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Ningbo Health Gene Technologies Ltd. Ningbo, Zhejiang, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Yanran
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Gong
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Ruidong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Ningbo Health Gene Technologies Ltd. Ningbo, Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Nan
authors.affiliation: Ningbo Health Gene Technologies Ltd. Ningbo, Zhejiang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Shilai
authors.initials: S
authors.lastname: Bao
authors.affiliation: CAS Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences. Shanghai, China.
authors.forename: Jing-Dong J
authors.initials: JD
authors.lastname: Han
authors.affiliation: Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics, Ministry of Education, Key Laboratory of Major Diseases in Children, Ministry of Education, Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University. Beijing, China.
authors.forename: Huyong
authors.initials: H
authors.lastname: Zheng
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002675:Child, Preschool; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007223:Infant; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor