title: ROLE OF SERUM AND ION CHANNEL BLOCK ON GROWTH AND HORMONALLY-INDUCED DIFFERENTIATION OF Spodoptera frugiperda (Sf21) INSECT CELLS.
abstract: A neuronal morphological phenotype can be induced in cultured Spodoptera frugiperda insect cells (Sf21) by supplementing serum-containing media with 20-hydroxyecdysone (20-HE) and/or insulin. In this study, the primary objectives were to determine any role of ion channels in mediating the morphological change in cells treated with 20-HE and insulin, and whether serum was required to observe this effect. Results showed serum-free media also induced growth of processes in Sf21 cells, but at a lower percentage than that found previously in cells bathed in serum-containing media. Veratridine, a sodium channel activator, increased cell survival when applied in combination with 20-HE to Sf21 cells, and the effect was blocked by tetrodotoxin (1 μM) a known sodium channel blocker. Cobalt, a calcium channel blocker, showed significant inhibition of cell process growth when applied in combination with both 20-HE and 20-HE plus veratridine. Cobalt also showed significant inhibition of cell process growth when applied in combination with insulin. Thus, some type of sodium channel, as well as a mechanism for transmembrane calcium ion movement, are apparently expressed in Sf21 cells and are involved in the differentiation process. These cell lines may be used in a wide variety of endeavors, including the screening of insecticides, as well as foster basic studies of neurodevelopment and ecdysone action.
authors.affiliation: Neurotoxicology Laboratory, Emerging Pathogens Institute, Department of Entomology and Nematology, University of Florida, Gainesville, Florida, USA.
authors.forename: Lacey J
authors.initials: LJ
authors.lastname: Jenson
authors.affiliation: Neurotoxicology Laboratory, Emerging Pathogens Institute, Department of Entomology and Nematology, University of Florida, Gainesville, Florida, USA.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Bloomquist
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003035:Cobalt / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D004441:Ecdysterone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007473:Ion Channels / Q000037:antagonists & inhibitors*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D044967:Serum; D061987:Sf9 Cells; D026941:Sodium Channel Blockers / Q000494:pharmacology; D018411:Spodoptera; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D016895:Culture Media, Serum-Free; D007328:Insulin; D007473:Ion Channels; D026941:Sodium Channel Blockers; D003035:Cobalt; D013779:Tetrodotoxin; D004441:Ecdysterone; D014701:Veratridine
keywords: 20-hydroxyecdysone; cobalt ion; sodium channel; veratridine