title: The Stability of Prostate-Specific Antigen in Semen Under Various Temperatures.
abstract: Prostate-specific antigen (PSA) is most commonly used for identifying semen, especially in the absence of sperm. However, PSA concentration varies according to storage temperature and duration, and little is known about its stability in semen. This study was therefore aimed to determine the stability under five different temperatures: -80, -20, 4, 25, and 37°C; and nine different durations: 1, 2, 3, 5, 7, 14, 30, 90, and 180 days. All samples were stored at -80°C after being secreted from the volunteers' body until analyzed. Results showed that the PSA concentration declined significantly over time under all temperatures studied except -80°C. At -20 and 4°C, PSA was still detectable on Day 180 with 50% and 70% decrease from its original concentration, respectively. At 25 and 37°C, PSA was detected up to Day 7 and 3, respectively. This information might assist forensic scientists understand more about PSA nature and integrate it into their works.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Bangkok, 10700, Thailand.
authors.forename: Supawon
authors.initials: S
authors.lastname: Srettabunjong
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Bangkok, 10700, Thailand.
authors.forename: Parimol
authors.initials: P
authors.lastname: Betset
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Bangkok, 10700, Thailand.
authors.forename: Suvit
authors.initials: S
authors.lastname: Limawongpranee
authors.affiliation: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Bangkok, 10700, Thailand.
authors.forename: Pattama
authors.initials: P
authors.lastname: Ekpo
mesh_terms: D005554:Forensic Medicine; D006801:Humans; D008297:Male; D011446:Prospective Studies; D017430:Prostate-Specific Antigen / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D013048:Specimen Handling; D013696:Temperature
chemical_list: D017430:Prostate-Specific Antigen
keywords: enzyme-linked immunosorbent assay; forensic biology; forensic science; prostate-specific antigen; semen; stability