title: Bilirubin Oxidase from Myrothecium verrucaria Physically Absorbed on Graphite Electrodes. Insights into the Alternative Resting Form and the Sources of Activity Loss.
abstract: The oxygen reduction reaction is one of the most important chemical processes in energy converting systems and living organisms. Mediator-less, direct electro-catalytic reduction of oxygen to water was achieved on spectrographite electrodes modified by physical adsorption of bilirubin oxidases from Myrothecium verrucaria. The existence of an alternative resting form of the enzyme is validated. The effect on the catalytic cycle of temperature, pH and the presence of halogens in the buffer was investigated. Previous results on the electrochemistry of bilirubin oxidase and on the impact of the presence of halogens are reviewed and reinterpreted.
authors.affiliation: Departamento de Química de los Materiales, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, RM, Chile.
authors.forename: Federico
authors.initials: F
authors.lastname: Tasca
authors.affiliation: Facultad de Ciencias Naturales, Matemáticas y Medioambiente, Universidad Tecnológica Metropolitana, Santiago, RM, Chile.
authors.forename: Diego
authors.initials: D
authors.lastname: Farias
authors.affiliation: Departamento de Química de los Materiales, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, RM, Chile.
authors.forename: Carmen
authors.initials: C
authors.lastname: Castro
authors.affiliation: Departamento de Química de los Materiales, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, RM, Chile.
authors.forename: Cristina
authors.initials: C
authors.lastname: Acuna-Rougier
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques; D020134:Catalytic Domain; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006999:Hypocreales / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D008958:Models, Molecular; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase