title: A Hyperlipidic Diet Combined with Short-Term Ovariectomy Increases Adiposity and Hyperleptinemia and Decreases Cytokine Content in Mesenteric Adipose Tissue.
abstract: Four-week-old female Wistar rats were divided into two groups and fed a control diet (C) or a hyperlipidic diet (H) for 4 weeks. Rats from each group underwent ovariectomy (OVX) or sham surgery (SHAM). They received C or H for the next four weeks. The body weight gain (BW), food efficiency (FE), and carcass lipid content were higher in the OVX H than in the SHAM H. The OVX H exhibited a higher serum leptin level than other groups. IL-6, TNF-α, and IL-10 content of mesenteric (MES) adipose tissue was lower in the OVX H than in the OVX C. IL-6, TNF-α, and IL-10 content of retroperitoneal (RET) adipose tissue was lower in the SHAM H than in the SHAM C. The SHAM H showed decreased TG relative to the SHAM C. Similar results were obtained in relation to IL-6Rα, TNFR1, TLR-4, and MyD88 contents in the MES and RET white adipose tissue among the groups. A hyperlipidic diet for 8 weeks combined with short-term ovariectomy decreases the cytokine content of MES adipose tissues but increases BW, enhancing FE and elevating serum leptin levels. These suggest that the absence of estrogens promotes metabolic changes that may contribute to installation of a proinflammatory process induced by a hyperlipidic diet.
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Nelson Inacio Pinto
authors.initials: NI
authors.lastname: Neto
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Maria Elizabeth Sousa
authors.initials: ME
authors.lastname: Rodrigues
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Ana Claudia Losinskas
authors.initials: AC
authors.lastname: Hachul
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Mayara Franzoi
authors.initials: MF
authors.lastname: Moreno
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Valter Tadeu
authors.initials: VT
authors.lastname: Boldarine
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Eliane Beraldi
authors.initials: EB
authors.lastname: Ribeiro
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Lila Missae
authors.initials: LM
authors.lastname: Oyama
authors.affiliation: Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo, Rua Botucatu 862, 2nd floor, Edifício de Ciências Biomédicas, 04023060 Vila Clementino, SP, Brazil.
authors.forename: Claudia Maria
authors.initials: CM
authors.lastname: Oller do Nascimento
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D050154:Adiposity; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D016207:Cytokines / Q000032:analysis*; D004032:Diet; D005260:Female; D006949:Hyperlipidemias / Q000209:etiology*; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008643:Mesentery / Q000276:immunology; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D007328:Insulin; D020738:Leptin