title: Studying centrosome function using three-dimensional cell cultures.
abstract: Three-dimensional (3D) cell cultures have long been recognized as a tool for the study of tissue architecture, polarity, and invasion. However, only recently these systems have been used to study centrosome and cilia functions. Studying these organelles in 3D cultures has elucidated new functions that otherwise would have been overlooked, demonstrating the value of these experimental systems to the field. Here we describe a culture method to study mammary epithelial cells in a 3D environment.
authors.affiliation: Barts Cancer Institute, Queen Mary University of London, London, UK.
authors.forename: Teresa
authors.initials: T
authors.lastname: Arnandis
authors.affiliation: Barts Cancer Institute, Queen Mary University of London, London, UK.
authors.forename: Susana A
authors.initials: SA
authors.lastname: Godinho
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D018385:Centrosome / Q000648:ultrastructure*; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D042361:Mammary Glands, Human / Q000166:cytology; D008856:Microscopy, Fluorescence; D018874:Spheroids, Cellular / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D020100:Hydrogels
keywords: Acini; Centrosome; MCF-10A; Mammary epithelial gland; Three-dimensional cultures