title: Influence of preoperative supplementation of omega-3 fatty acid in the healing of colonic anastomoses in malnourished rats receiving paclitaxel.
abstract: OBJECTIVE
  To evaluate the effect of preoperative supplementation of omega-3 fatty acids on the healing of colonic anastomoses in malnourished rats receiving paclitaxel.
  
  
  METHODS
  we studied 160 male Wistar rats, divided in two groups: one subjected to malnutrition by pair feeding (M) for four weeks, and another that received food ad libitum (W). In the fourth week, the groups were further divided into two subgroups that received omega-3 or olive oil by gavage. The animals were submitted to colonic transection and end-to-end anastomosis. After the operation, each of the four groups was divided into two subgroups that received intraperitoneal isovolumetric solutions of saline or paclitaxel.
  
  
  RESULTS
  mortality was 26.8% higher in the group of animals that received paclitaxel (p = 0.003). The complete rupture strength was greater in well-nourished-oil Paclitaxel group (WOP) compared with the the malnourished-oil Paclitaxel one (MOP). The collagen maturation index was higher in well-nourished-oil saline group (WOS) in relation to the malnutrition-oil-saline group (MOS), lower in malnourished-oil-saline group (MOS) in relation to malnourished-ômega3-saline one (M3S) and lower in the well-nourished-omega3-saline group (W3S) compared with the malnourished-omega3-saline (M3S). The blood vessel count was higher in the malnourished-oil-saline group (MOS) than in the malnourished-oil-paclitaxel group (MOP) and lower in the malnourished-oil-saline group (MOS) in relation to the malnourished-omega3-paclitaxel group (M3P).
  
  
  CONCLUSIONS
  supplementation with omega-3 fatty acids was associated with a significant increase in the production of mature collagen in malnourished animals, with a reversal of the harmful effects caused by malnutrition associated with the use of paclitaxel on the rupture strength, and with a stimulus to neoangiogenesis in the group receiving paclitaxel.
authors.affiliation: Hospital Santa Rita de Maringá, Maringá, Paraná, Brazil.
authors.forename: Alvo Orlando
authors.initials: AO
authors.lastname: Vizzotto Junior
authors.affiliation: Departamento de Cirurgia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: Antonio Carlos Ligocki
authors.initials: AC
authors.lastname: Campos
authors.affiliation: Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: Eneri Vieira de Souza Leite
authors.initials: EV
authors.lastname: Mello
authors.affiliation: Hospital Santa Rita de Maringá, Maringá, Paraná, Brazil.
authors.forename: Tiago Jacometo
authors.initials: TJ
authors.lastname: Castilho
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D003106:Colon / Q000601:surgery*; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D008297:Male; D044342:Malnutrition / Q000188:drug therapy*; D017239:Paclitaxel / Q000627:therapeutic use*; D011300:Preoperative Care; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D015525:Fatty Acids, Omega-3; D017239:Paclitaxel