title: Enhancing the selective extracellular location of a recombinant E. coli domain antibody by management of fermentation conditions.
abstract: The preparation of a recombinant protein using Escherichia coli often involves a challenging primary recovery sequence. This is due to the inability to secrete the protein to the extracellular space without a significant degree of cell lysis. This results in the release of nucleic acids, leading to a high viscosity, difficulty to clarify, broth and also to contamination with cell materials such as lipopolysaccharides and host cell proteins. In this paper, we present different fermentation strategies to facilitate the recovery of a V H domain antibody (13.1 kDa) by directing it selectively to the extracellular space and changing the balance between domain antibody to nucleic acid release. The manipulation of the cell growth rate in order to increase the outer cell membrane permeability gave a small ~1.5-fold improvement in released domain antibody to nucleic acid ratio without overall loss of yield. The introduction during fermentation of release agents such as EDTA gave no improvement in the ratio of released domain antibody to nucleic acid and a loss of overall productivity. The use of polyethyleneimine (PEI) during fermentation was with the aim to (a) permeabilise the outer bacterial membrane to release selectively domain antibody and (b) remove selectively by precipitation nucleic acids released during cell lysis. This strategy resulted in up to ~4-fold increase in the ratio of domain antibody to soluble nucleic acid with no reduction in domain antibody overall titre. In addition, a reduction in host cell protein contamination was achieved and there was no increase in endotoxin levels. Similar results were demonstrated with a range of other antibody products prepared in E. coli.
authors.affiliation: BioPharm R&D, BioPharm Process Research, GlaxoSmithKline R&D, Stevenage, SG1 2NY, UK.|The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Voulgaris
authors.affiliation: BioPharm R&D, BioPharm Process Research, GlaxoSmithKline R&D, Stevenage, SG1 2NY, UK.
authors.forename: Gary
authors.initials: G
authors.lastname: Finka
authors.affiliation: BioPharm R&D, BioPharm Process Research, GlaxoSmithKline R&D, Stevenage, SG1 2NY, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Uden
authors.affiliation: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK. m.hoare@ucl.ac.uk.
authors.forename: Mike
authors.initials: M
authors.lastname: Hoare
mesh_terms: D000906:Antibodies / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D010539:Permeability / Q000187:drug effects; D011094:Polyethyleneimine / Q000378:metabolism; D021381:Protein Transport; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D003470:Culture Media; D011994:Recombinant Proteins; D011094:Polyethyleneimine
keywords: Domain antibody; E. coli; Fermentation; Periplasmic release; Polyethyleneimine