title: miR-107 promotes hepatocellular carcinoma cell proliferation by targeting Axin2.
abstract: BACKGROUND
  A large number of studies demonstrated that microRNAs play important roles in the progression and development of human cancers. However, the expression level of miR-107 and its biological function in hepatocellular carcinoma (HCC) remains unclear.
  
  
  METHODS
  Quantitative real-time PCR (qRT-PCR) was used to evaluate the expression level of miR-107 in HCC tissues and cell lines. Then, we explored the function of miR-107 to determine its potential roles on HCC cell proliferation in vitro. Luciferase reporter assay was used to confirm the target gene of miR-107, and the results were validated in cell lines.
  
  
  RESULTS
  miR-107 was significantly up-regulated in HCC tissues and cell lines. The enforced expression of miR-107 was able to promote cell proliferation in HepG2 cells. At the molecular level, our results suggested that expression of Axin2 was negatively regulated by miR-107.
  
  
  CONCLUSIONS
  Our observations suggested that miR-107 could promote HCC cells proliferation via targeting Axin2 and might represent a potential therapeutic target for HCC.
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, 475000, Henan Province, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, 475000, Henan Province, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, 475000, Henan Province, China.
authors.forename: Long
authors.initials: L
authors.lastname: Hua
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, 475000, Henan Province, China.
authors.forename: Kun-Hou
authors.initials: KH
authors.lastname: Yao
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, 475000, Henan Province, China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, 475000, Henan Province, China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Hu
mesh_terms: D060466:Axin Protein / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: C491761:AXIN2 protein, human; D060466:Axin Protein; C549045:MIRN107 microRNA, human; D035683:MicroRNAs; D008156:Luciferases
keywords: Axin2; Hepatocellular carcinoma; miR-107; proliferation