title: Synthesis of mouse centromere-targeted polyamides and physico-chemical studies of their interaction with the target double-stranded DNA.
abstract: Synthetic minor groove-binding pyrrole-imidazole polyamides labeled by fluorophores are promising candidates for fluorescence imaging of double-stranded DNA in isolated chromosomes or fixed and living cells. We synthesized nine hairpin and two head-to-head tandem polyamides targeting repeated sequences from mouse major satellites. Their interaction with synthetic target dsDNA has been studied by physico-chemical methods in vitro before and after coupling to various fluorophores. Great variability in affinities and fluorescence properties reveals a conclusion that these properties do not only rely on recognition rules, but also on other known and unknown structural factors. Individual testing of each probe is needed before cellular applications.
authors.affiliation: Structure and Instability of Genomes, Sorbonne Universités, Muséum National d'Histoire Naturelle, INSERM U 1154, CNRS UMR 7196, 57 rue Cuvier, C.P. 26, 75231 Paris cedex 05, France. Electronic address: karine.nozeret@gmail.com.
authors.forename: Karine
authors.initials: K
authors.lastname: Nozeret
authors.affiliation: Structure and Instability of Genomes, Sorbonne Universités, Muséum National d'Histoire Naturelle, INSERM U 1154, CNRS UMR 7196, 57 rue Cuvier, C.P. 26, 75231 Paris cedex 05, France; Université Paris René Descartes, 12 Rue de l'École de Médecine, 75006 Paris, France. Electronic address: bonan_marc@yahoo.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Bonan
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, ul. Zabolotnogo, 150, P.O. 88, 03187 Kiev, Ukraine. Electronic address: yarmoluksm@gmail.com.
authors.forename: Serguiy M
authors.initials: SM
authors.lastname: Yarmoluk
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Lavrentyev prosp., 8, 630090 Novosibirsk, Russia. Electronic address: danov@niboch.nsc.ru.
authors.forename: Darya S
authors.initials: DS
authors.lastname: Novopashina
authors.affiliation: Structure and Instability of Genomes, Sorbonne Universités, Muséum National d'Histoire Naturelle, INSERM U 1154, CNRS UMR 7196, 57 rue Cuvier, C.P. 26, 75231 Paris cedex 05, France. Electronic address: alexandre.boutorine@mnhn.fr.
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: Boutorine
mesh_terms: D000818:Animals; D001665:Binding Sites; D002503:Centromere / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D051379:Mice; D009690:Nucleic Acid Conformation; D009757:Nylons / Q000737:chemistry*
chemical_list: D009757:Nylons; D004247:DNA
keywords: Binding affinity; DNA recognition; Fluorescent probes; Minor-groove binders; Polyamide tandem; Pyrrole–imidazole polyamides