title: Prophylactic strategies for acute hemolysis secondary to plasma-incompatible platelet transfusions: correlation between qualitative hemolysin test and isohemagglutinin titration.
abstract: OBJECTIVE
  Brazilian legislation has recently suggested the use of the qualitative hemolysin test instead of isohemagglutinin titers as prophylaxis for acute hemolysis related to plasma-incompatible platelet transfusions. The efficacy of this test in preventing hemolytic reactions has never been evaluated while isohemagglutinin titers have been extensively studied. The main objective of this study was to evaluate the correlation between the results of these two tests. The impact of each type of prophylaxis on the platelet inventory management and the ability of the qualitative hemolysin test to prevent red cell sensitization after the transfusion of incompatible units were also studied.
  
  
  METHODS
  A total of 246 donor blood samples were evaluated using both isohemagglutinin titers and the qualitative hemolysin test, and the results were statistically compared. Subsequently, 600 platelet units were tested using the hemolysin assay and the percentage of units unsuitable for transfusion was compared to historical data using isohemagglutinin titers (cut-off: 100). Moreover, ten patients who received units with minor ABO incompatibilities that were negative for hemolysis according to the qualitative hemolysin test were evaluated regarding the development of hemolysis and red cell sensitization (anti-A or anti-B).
  
  
  RESULTS
  Isohemagglutinin titration and the results of qualitative hemolysin test did not correlate. The routine implementation of the qualitative hemolysin test significantly increased the percentage of platelet units found unsuitable for transfusions (15-65%; p-value <0.001). Furthermore the qualitative hemolysin test did not prevent red blood cell sensitization in a small exploratory analysis.
  
  
  CONCLUSIONS
  Qualitative hemolysin test results do not correlate to those of isohemagglutinin titers and its implementation as the prophylaxis of choice for hemolysis associated with plasma-incompatible platelet transfusions lacks clinical support of safety and significantly affects platelet inventory management.
authors.affiliation: Fundação Pró-Sangue, Hemocentro de São Paulo, São Paulo, SP, Brazil.
authors.forename: Cinthia Silvestre
authors.initials: CS
authors.lastname: Landim
authors.affiliation: Fundação Pró-Sangue, Hemocentro de São Paulo, São Paulo, SP, Brazil.
authors.forename: Francisco Carlos Almeida
authors.initials: FC
authors.lastname: Gomes
authors.affiliation: Fundação Pró-Sangue, Hemocentro de São Paulo, São Paulo, SP, Brazil.
authors.forename: Bernardete Martin
authors.initials: BM
authors.lastname: Zeza
authors.affiliation: Fundação Pró-Sangue, Hemocentro de São Paulo, São Paulo, SP, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Mendrone-Júnior
authors.affiliation: Fundação Pró-Sangue, Hemocentro de São Paulo, São Paulo, SP, Brazil. Electronic address: caludinardo@gmail.com.
authors.forename: Carla Luana
authors.initials: CL
authors.lastname: Dinardo
keywords: ABO blood-group system; Blood group incompatibility; Hemolysin proteins; Hemolysis; Platelet transfusion