title: In vitro anti-denaturation and anti-hyaluronidase activities of extracts and galactolipids from leaves of Impatiens parviflora DC.
abstract: The in vitro anti-denaturation and anti-hyaluronidase activities of Impatiens parviflora extracts and isolated galactolipids (MGDG-1, DGDG-1) were investigated. This is the first report on these compounds in I. parviflora. All extracts showed anti-hyaluronidase activity, but only methanolic extract from fresh leaves exhibited significant activity against heat-induced denaturation of BSA in a dose-dependent manner. At 500 μg/mL, the extract and the reference drug showed 79.05% and 99.81% inhibition of protein denaturation, respectively. These results indicate that fresh leaves of I. parviflora may be beneficial in inflammatory conditions, especially those associated with protein denaturation, such as rheumatoid arthritis. The study revealed that only MGDG-1 showed weak activity in anti-denaturation assay but both galactolipids were potent inhibitors of hyaluronidase. MGDG-1 completely inhibited the enzyme activity at the concentration of 127.9 μg/mL. These results indicate the potential of galactolipids in the treatment of diseases associated with the loss of hyaluronic acid.
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Grabowska
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Irma
authors.initials: I
authors.lastname: Podolak
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Galanty
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Załuski
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Makowska-Wąs
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Sobolewska
authors.affiliation: a Department of Pharmacognosy , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Janeczko
authors.affiliation: b Department of Medicinal Chemistry , Pharmaceutical Faculty, Medical College, Jagiellonian University , Medyczna 9, 30-688 Cracow , Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Żmudzki
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D038983:Galactolipids / Q000302:isolation & purification / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D029069:Impatiens / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D038983:Galactolipids; D010936:Plant Extracts; D006821:Hyaluronoglucosaminidase
keywords: (DGDG) digalactosyldiacylglicerol; (MGDG) monogalactosyldiacylglicerol; Impatiens parviflora; anti-arthritic; anti-denaturation; anti-hyaluronidase