title: Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss Sphagnum fuscum.
abstract: Using a culture-based nitrous oxide (N2O) emission assay, three active N2O emitters were isolated from Sphagnum fuscum leaves and all identified as members of Burkholderia. These isolates showed N2O emission in the medium supplemented with [Formula: see text] but not with [Formula: see text], and Burkholderia sp. SF-E2 showed the most efficient N2O emission (0.20 μg·vial(-1)·day(-1)) at 1.0 mM KNO3. In Burkholderia sp. SF-E2, the optimum pH for N2O production was 5.0, close to that of the phyllosphere of Sphagnum mosses, while the optimum temperature was uniquely over 30 °C. The stimulating effect of additional 1.5 mM sucrose on N2O emission was ignorable, but Burkholderia sp. SF-E2 upon exposure to 100 mg·L(-1) E-caffeic acid showed uniquely 67-fold higher N2O emission. All of the three N2O emitters were negative in both acetylene inhibition assay and PCR assay for nosZ-detection, suggesting that N2O reductase or the gene itself is missing in the N2O-emitting Burkholderia.
authors.affiliation: a Graduate School of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Nie
authors.affiliation: a Graduate School of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: a Graduate School of Agriculture , Hokkaido University , Sapporo , Japan.|b Institute of Pesticide and Environmental Toxicology , Zhejiang University , Hangzhou , China.
authors.forename: Mengcen
authors.initials: M
authors.lastname: Wang
authors.affiliation: c Department of Biology , University of Eastern Finland , Joensuu , Finland.
authors.forename: Teemu
authors.initials: T
authors.lastname: Tahvanainen
authors.affiliation: a Graduate School of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hashidoko
mesh_terms: D000114:Acetylene / Q000494:pharmacology; D019117:Burkholderia / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009609:Nitrous Oxide / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000382:microbiology*; D044003:Sphagnopsida / Q000382:microbiology*; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
chemical_list: D013395:Sucrose; D009609:Nitrous Oxide; D000114:Acetylene
keywords: Burkholderia; Sphagnum fuscum; Sphagnum moss polyphenols; nitrous oxide (N2O) emitter; nosZ gene