title: Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads.
abstract: Saliva diagnostics utilizing nanotechnology and molecular technologies to detect oral squamous cell carcinoma (OSCC) has become an attractive field of study. However, no specific methods have been established. To refine the diagnostic power of saliva peptide fingerprints for the early detection of OSCC, we screened the expression spectrum of salivary peptides in 40 T1 stage OSCC patients (and healthy controls) using MALDI-TOF-MS combined with magnetic beads. Fifty proteins showed significantly different expression levels in the OSCC samples (P<0.05). Potential biomarkers were also predicted. The novel diagnostic proteomic model with m/z peaks of 1285.6 Da and 1432.2 Da are of certain value for early diagnosis of OSCC.
authors.affiliation: The Third Dental Center, Peking University School and Hospital of Stomatology, Beijing 100083, PR China.
authors.forename: Wei-Peng
authors.initials: WP
authors.lastname: Jiang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Third Dental Center, Peking University School and Hospital of Stomatology, Beijing 100083, PR China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Xu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, PR China moleculecf@gmail.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D010449:Peptide Mapping / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000379:methods; D012463:Saliva / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor
keywords: early diagnosis; histatin-3; matrix-assisted laser-desorption ionization–time-of-flight–mass spectrometry (MALDI-TOF-MS); oral squamous cell carcinoma (OSCC); peptides; saliva