title: Interaction of human mannose-binding lectin (MBL) with Yersinia enterocolitica lipopolysaccharide.
abstract: The lipopolysaccharide (LPS) is involved in the interaction between Gram-negative pathogenic bacteria and host. Mannose-binding lectin (MBL), complement-activating soluble pattern-recognition receptor targets microbial glycoconjugates, including LPS. We studied its interactions with a set of Yersinia enterocolitica O:3 LPS mutants. The wild-type strain LPS consists of lipid A (LA) substituted with an inner core oligosaccharide (IC) which in turn is substituted either with the O-specific polysaccharide (OPS) or the outer core hexasaccharide (OC), and sometimes also with the enterobacterial common antigen (ECA). The LPS mutants produced truncated LPS, missing OPS, OC or both, or, in addition, different IC constituents or ECA. MBL bound to LA-IC, LA-IC-OPS and LA-IC-ECA but not LA-IC-OC structures. Moreover, LA-IC substitution with both OPS and ECA prevented the lectin binding. Sequential truncation of the IC heptoses demonstrated that the MBL targets the IC heptose region. Furthermore, microbial growth temperature influenced MBL binding; binding was stronger to bacteria grown at room temperature (22°C) than to bacteria grown at 37°C. In conclusion, our results demonstrate that MBL can interact with Y. enterocolitica LPS, however, the in vivo significance of that interaction remains to be elucidated.
authors.affiliation: Department of Microbiology, University of Silesia, Jagiellonska 28, PL 40-032 Katowice, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kasperkiewicz
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, PL 93-232 Lodz, Poland.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Swierzko
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, PL 93-232 Lodz, Poland.
authors.forename: Marcin A
authors.initials: MA
authors.lastname: Bartlomiejczyk
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, PL 93-232 Lodz, Poland. Electronic address: mcedzynski@cbm.pan.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Cedzynski
authors.affiliation: Department of Microbiology, University of Silesia, Jagiellonska 28, PL 40-032 Katowice, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Noszczynska
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Priority Area Asthma and Allergies, Leibniz Center for Medicine and Biosciences, Parkallee 4a/c, D 23845 Borstel, Germany.
authors.forename: Katarzyna A
authors.initials: KA
authors.lastname: Duda
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, PL 93-232 Lodz, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Michalski
authors.affiliation: Department of Bacteriology and Immunology, Haartman Institute, Research Programs Unit, Immunobiology, University of Helsinki, PO Box 21, Haartmaninkatu 3, FIN 00014 Helsinki, Finland; Helsinki University Central Hospital Laboratory Diagnostics, PO Box 21, Haartmaninkatu 3, FIN 00014 Helsinki, Finland.
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
mesh_terms: D000942:Antigens, Bacterial / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D037601:Mannose-Binding Lectin / Q000097:blood*; D019081:O Antigens / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000235:genetics / Q000254:growth & development*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D037601:Mannose-Binding Lectin; D019081:O Antigens; C017286:enterobacterial common antigen
keywords: Complement; Lipopolysaccharide (LPS); MBL); Mannose-binding lectin (mannan-binding lectin; Rough mutants; Yersinia