title: A trifunctional, triangular RNA-protein complex.
abstract: Multifunctional molecular complexes are valuable tools with a variety of applications. We have developed an RNA-protein complex (RNP) containing three different proteins attached to the tips of a triangular RNA scaffold. We designed and constructed three RNA strands that specifically bind a ribosomal protein, L7Ae, and that autonomously form a single triangular RNP via RNA kissing loop (KL) interactions. This RNP-based approach can be used as an alternative tool to produce unique, multifunctional molecules with customized dimensions, functions, and targets.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Shoji J
authors.initials: SJ
authors.lastname: Ohuchi
authors.affiliation: Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sagawa
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
authors.forename: Taiichi
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: tan@kuchem.kyoto-u.ac.jp.
authors.forename: Tan
authors.initials: T
authors.lastname: Inoue
mesh_terms: D001483:Base Sequence; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D012269:Ribosomal Proteins; D012313:RNA
keywords: Kissing loop interaction; RNA–protein complex; RNP nano-object