title: Development of a rapid, reliable and quantitative method--"SPOTi" for testing antifungal efficacy.
abstract: A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.
authors.affiliation: Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK; Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
authors.forename: Khalida
authors.initials: K
authors.lastname: Rizi
authors.affiliation: Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Sudaxshina
authors.initials: S
authors.lastname: Murdan
authors.affiliation: Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Danquah
authors.affiliation: Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
authors.forename: Jane
authors.initials: J
authors.lastname: Faull
authors.affiliation: Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK. Electronic address: s.bhakta@bbk.ac.uk.
authors.forename: Sanjib
authors.initials: S
authors.lastname: Bhakta
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects; D055808:Drug Discovery / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000935:Antifungal Agents
keywords: Antifungals; Dermatophytes; Drug discovery; SPOTi; Screening