title: Roles of the three L-domains in β-retrovirus budding.
abstract: Retroviral Gag protein plays a critical role during the late stage of virus budding and possesses a so-called L-domain containing PT/SAP, PPxY, YxxL or FPIV motifs that are critical for efficient budding. Mason-Pfizer monkey virus (M-PMV) contains PSAP, PPPY, and YADL sequences in Gag. This study was performed to investigate the roles of these three L-domain-like sequences in virus replication in three different cell lines, 293T, COS-7 and HeLa cells. It was found that the PPxY motif plays an essential role in progeny virus production as a major L-domain in all three cell lines. The PSAP sequence was shown to function as an additional L-domain in HeLa cells and to promote efficient release of M-PMV; however, this sequence was dispensable for M-PMV production in 293T and COS-7 cells, suggesting that the role of the PSAP motif as an L-domain in M-PMV budding is cell type-dependent. Viruses possessing multiple L-domains appear to change the L-domain usage to replicate in various cells. On the other hand, the YADL motif was required for M-PMV production as a transport signal of Gag to the plasma membrane, but not as an L-domain.
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN).|Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Narahara
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN).|Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D015683:Gene Products, gag / Q000378:metabolism*; D006801:Humans; D017434:Protein Structure, Tertiary; D015301:Retroviruses, Simian / Q000502:physiology*; D057074:Virus Release
chemical_list: D015683:Gene Products, gag
keywords: L-domain; M-PMV; budding; retrovirus