title: [Maldi-tof ms analysis for yersinia pestis, vibrio cholera, and francisella tularensis identification].
abstract: Numerous studies showed that a new technology for the clinical microbiology laboratories, Matrix-Assisted Laser Desorption Ionization--Time of Flight Mass Spectrometry (MALDI-ToF MS), allows fast, accurate, and effective identification of most clinically relevant microorganisms to be implemented. In the present review, we discuss applications of this approach for identification and typing of extremely dangerous pathogens--Yersinia pestis, Vibrio cholera, and Francisella tularensis, including the advantages and disadvantages of the method, sample preparation and biosafety problems.
authors.forename: M V
authors.initials: MV
authors.lastname: Afanas'ev
authors.forename: L V
authors.initials: LV
authors.lastname: Mironova
authors.forename: S V
authors.initials: SV
authors.lastname: Balakhonov
mesh_terms: D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques / Q000379:methods; D002771:Cholera / Q000382:microbiology; D003264:Containment of Biohazards / Q000379:methods; D005604:Francisella tularensis / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D013048:Specimen Handling / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014733:Vibrio / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D014734:Vibrio cholerae / Q000302:isolation & purification* / Q000472:pathogenicity; D015007:Yersinia / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D015010:Yersinia pestis / Q000302:isolation & purification* / Q000472:pathogenicity