title: Prenatal stress induces vulnerability to nicotine addiction and alters D2 receptors' expression in the nucleus accumbens in adult rats.
abstract: Prenatal stress (PS) can induce several long-lasting behavioral and molecular abnormalities in rats. It can also be considered as a risk factor for many psychiatric diseases like schizophrenia, depression or PTSD and predispose to addiction. In this study, we investigated the effect of prenatal stress on the reinforcing properties of nicotine in the CPP paradigm. Then, we examined the mRNA expression of the D2 dopaminergic receptors using the quantitative real-time PCR technique in the nucleus accumbens (NAcc). We found that prenatally stressed rats exhibited a greater place preference for the nicotine-paired compartment than the control rats. Moreover, we observed an overexpression of the DRD2 gene in adult offspring stressed in utero and a downregulation in the PS NIC group (PS rats treated with nicotine) compared with their control counterparts (C NIC). These data suggest that maternal stress can permanently alter the offspring's addictive behavior and D2 receptors' expression.
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco. Electronic address: s.nadia.10@hotmail.fr.
authors.forename: N
authors.initials: N
authors.lastname: Said
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: S
authors.initials: S
authors.lastname: Lakehayli
authors.affiliation: Genetics and Molecular Pathology Laboratory, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: El Khachibi
authors.affiliation: Sultan Moulay Slimane University, Faculty of Sciences & Techniques Beni-Mellal, Life Sciences, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: El Ouahli
authors.affiliation: Genetics and Molecular Pathology Laboratory, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: S
authors.initials: S
authors.lastname: Nadifi
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: F
authors.initials: F
authors.lastname: Hakkou
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Tazi
mesh_terms: D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D009538:Nicotine / Q000008:administration & dosage; D018722:Nicotinic Agonists / Q000008:administration & dosage; D009714:Nucleus Accumbens / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D013037:Spatial Behavior / Q000187:drug effects / Q000502:physiology; D013315:Stress, Psychological / Q000503:physiopathology*; D014029:Tobacco Use Disorder / Q000503:physiopathology*
chemical_list: C000607792:DRD2 protein, rat; D018722:Nicotinic Agonists; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D009538:Nicotine
keywords: DRD2 mRNA expression; conditioned place preference; nicotine; nucleus accumbens; prenatal stress; qPCR