title: Influence of Staphylococcus aureus attachment to the herpes simplex virus infected cells.
abstract: The purpose of this study was to investigate the response of HeLa cells to the interaction with inactivated Staphylococcus aureus cells and live challenge with herpes simplex virus (HSV).The results of this study are indicating that the interaction between the HeLa cells and S. aureus inactivated whole cells could modulate the host cell apoptosis and cytokine production, and therefore, influence the progression of HSV infection. The pre-treatment of HeLa cells with heat inactivated bacterial whole cells protects them from the occurrence of HSV mediated cytopathic effect, while the post viral infection treatment with bacterial cells prevents the high activation of bax/bcl-2 apoptotic pathway, a process that could change the fate of the infectious process triggered by the virus, and eventually reduce its multiplication rate. The pre-treatment of HeLa monolayer with inactivated bacterial cells 24 hours before the viral infection is increasing the expression level of TNF-a, IL-6 and IL-8 pro-inflammatory cytokines genes, also suggesting that bacterial antigens could contribute to the decrease of viral multiplication rate.
authors.affiliation: Clinic of ENT, Head and Neck Surgery, "Coltea" Clinical Hospital, Bucharest, Romania; raluca.grigore@3f.ro.
authors.forename: Coralia
authors.initials: C
authors.lastname: Bleotu
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.forename: Veronica
authors.initials: V
authors.lastname: Lazăr
authors.forename: Roxana
authors.initials: R
authors.lastname: Drăguşel
authors.forename: Lilia
authors.initials: L
authors.lastname: Matei
authors.forename: Ioana Mădălina
authors.initials: IM
authors.lastname: Aldea
authors.forename: Laura Denisa
authors.initials: LD
authors.lastname: Dragu
authors.forename: Bogdan
authors.initials: B
authors.lastname: Ionescu
authors.forename: Carmen Cristina
authors.initials: CC
authors.lastname: Diaconu
authors.forename: Costin
authors.initials: C
authors.lastname: Cernescu
authors.forename: Carmen Aurelia
authors.initials: CA
authors.lastname: Mogoantă
authors.forename: Cristian Radu
authors.initials: CR
authors.lastname: Popescu
authors.forename: Bogdan
authors.initials: B
authors.lastname: Popescu
authors.forename: Raluca
authors.initials: R
authors.lastname: Grigore
authors.forename: Şerban Vifor Gabriel
authors.initials: ŞV
authors.lastname: Berteşteanu
mesh_terms: D000956:Antigens, Viral / Q000378:metabolism; D017209:Apoptosis; D001422:Bacterial Adhesion; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006561:Herpes Simplex / Q000382:microbiology* / Q000821:virology*; D006801:Humans; D050296:Microbial Viability; D018139:Simplexvirus / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology*; D038301:Virus Inactivation; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000956:Antigens, Viral; D016207:Cytokines; D051028:bcl-2-Associated X Protein