title: Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia).
abstract: Short, Gram-negative-staining, rod-shaped bacteria were isolated from crushed bodies of Russian wheat aphid [Diuraphis noxia (Kurdjumov)] and artificial diets after Russian wheat aphid feeding. Based on multilocus sequence analysis involving the 16S rRNA, atpD, infB, gyrB and rpoB genes, these bacterial isolates constitute a novel clade in the genus Erwinia, and were most closely related to Erwinia toletana. Representative distinct strains within this clade were used for comparisons with related species of Erwinia. Phenotypic comparisons using four distinct strains and average nucleotide identity (ANI) measurements using two distinct draft genomes revealed that these strains form a novel species within the genus Erwinia. The name Erwinia iniecta sp. nov. is proposed, and strain B120T ( = CFBP 8182T = NCCB 100485T) was designated the type strain. Erwinia iniecta sp. nov. was not pathogenic to plants. However, virulence to the Russian wheat aphid was observed.
authors.affiliation: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523-1177, USA.|Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523-1177, USA.
authors.forename: Tony
authors.initials: T
authors.lastname: Campillo
authors.affiliation: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523-1177, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Luna
authors.affiliation: INRA, UMR1345 Institut de Recherche en Horticulture et Semences, Angers, France.|Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, Angers, France.|Université d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France.
authors.forename: Perrine
authors.initials: P
authors.lastname: Portier
authors.affiliation: INRA, UMR1345 Institut de Recherche en Horticulture et Semences, Angers, France.|Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, Angers, France.|Université d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Fischer-Le Saux
authors.affiliation: Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523-1177, USA.|Bureau for Food Security, US Agency for International Development, 1300 Pennsylvania Ave NW, Washington, DC 20523, USA.
authors.forename: Nora
authors.initials: N
authors.lastname: Lapitan
authors.affiliation: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523-1177, USA.
authors.forename: Ned A
authors.initials: NA
authors.lastname: Tisserat
authors.affiliation: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523-1177, USA.
authors.forename: Jan E
authors.initials: JE
authors.lastname: Leach
mesh_terms: D000818:Animals; D001042:Aphids / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004885:Erwinia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014908:Triticum
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S