title: Characterization of Arcobacter suis isolated from water buffalo (Bubalus bubalis) milk.
abstract: During a survey in a dairy plant in Italy, the second strain (strain FG 206) of Arcobacter suis described in the literature was isolated from raw water buffalo milk. The objective of this study was to confirm the species identification, better define the species by comparing its characteristics with those of the reference strain (F41(T) = CECT 7833(T) = LMG 26152(T)) and to investigate its potential clinical relevance by detecting the virulence gene pattern of the new strain. Phenotypical characterization and 16S rRNA-RFLP gave a complete overlap of results for the two strains. As expected, an RFLP pattern common to A. suis and Arcobacter defluvii was obtained by MseI endonuclease digestion, and a pattern specific for A. suis was obtained by BfaI endonuclease digestion. 16S rRNA sequencing and multilocus phylogenetic analysis (MLPA) showed a robust relatedness of strain FG 206 to the A. suis type strain F41(T). The recovery of strain FG 206 from a dairy plant shows that this species of Arcobacter is present in the food chain. Like the type strain recovered from pig meat, the species A. suis may not be confined to a single type of food.
authors.affiliation: Department of Veterinary Medical Sciences - University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy. Electronic address: federica.giacometti3@unibo.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Giacometti
authors.affiliation: Unitat de Microbiologia, Departament de Ciènces Médiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, 43201 Reus, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Salas-Massó
authors.affiliation: Department of Veterinary Medical Sciences - University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Serraino
authors.affiliation: Unitat de Microbiologia, Departament de Ciènces Médiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, 43201 Reus, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Figueras
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D002020:Buffaloes / Q000382:microbiology*; D007558:Italy; D008892:Milk / Q000382:microbiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Arcobacter suis; Milk; Typing; Virulence