title: An Arabidopsis Plasma Membrane Proton ATPase Modulates JA Signaling and Is Exploited by the Pseudomonas syringae Effector Protein AvrB for Stomatal Invasion.
abstract: Stomata are natural openings through which many pathogenic bacteria enter plants. Successful bacterial pathogens have evolved various virulence factors to promote stomatal opening. Here, we show that the Pseudomonas syringae type III effector protein AvrB induces stomatal opening and enhances bacterial virulence in a manner dependent on RPM1-INTERACTING4 (RIN4), which promotes stomatal opening by positively regulating the Arabidopsis plasma membrane H(+)-ATPase (AHA1), which is presumed to directly regulate guard cell turgor pressure. In support of a role of AHA1 in AvrB-induced stomatal opening, AvrB enhances ATPase activity in plants. Unexpectedly, AHA1 promotes the interaction between the jasmonate (JA) receptor CORONATINE INSENSITIVE1 (COI1) and JASMONATE ZIM-DOMAIN (JAZ) proteins and enhances JA signaling. JA signaling is required for optimum stomatal infection in AHA1-active plants. Similarly, AvrB also induces the COI1-JAZ9 interaction and the degradation of multiple JAZ proteins. AvrB-induced stomatal opening and virulence require the canonical JA signaling pathway, which involves the COI1 and NAC transcription factors. The findings thus point to a previously unknown pathway exploited by P. syringae that acts upstream of COI1 to regulate JA signaling and stomatal opening.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Du
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China.
authors.forename: Chuanyou
authors.identifier: http://orcid.org/0000-0003-0202-3890
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China jmzhou@genetics.ac.cn.
authors.forename: Jian-Min
authors.identifier: http://orcid.org/0000-0002-9943-2975
authors.initials: JM
authors.lastname: Zhou
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003517:Cyclopentanes / Q000378:metabolism*; D005816:Genetic Complementation Test; D007192:Indenes / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D054046:Plant Stomata / Q000187:drug effects / Q000382:microbiology*; D011485:Protein Binding / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000187:drug effects / Q000472:pathogenicity*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000378:metabolism; D014774:Virulence / Q000187:drug effects
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; C100931:AvrB protein, Pseudomonas syringae; D001426:Bacterial Proteins; D003517:Cyclopentanes; D007192:Indenes; D054883:Oxylipins; D014157:Transcription Factors; C058487:coronatine; C011006:jasmonic acid; D006180:Proton-Translocating ATPases; C544444:AHA1 protein, Arabidopsis