title: Simultaneous determination of seven bioactive components in Guizhi Fuling capsule by microwave-assisted extraction combined with ultra performance liquid chromatography tandem mass spectrometry.
abstract: A simple, rapid and reliable microwave-assisted extraction (MAE) combined with ultra performance liquid chromatography tandem mass spectrometry method was developed for simultaneous determination of the seven bioactive constituents in Guizhi Fuling capsule (GFC), namely gallic acid, amygdalin, albiflorin, paeoniflorin, paeonol, cinnamic acid and pachymic acid, respectively. The operation of MAE optimised through orthogonal array design experiment was performed at 80°C for 10 min with methanol-water (70:30, v/v) as the extracting solvent. The method was validated including intra- and inter-day precision, repeatability and stability, with relative standard deviation less than 3.9%, 3.3%, 4.4% and 3.1%, respectively. All analytes showed the good linearity (r >0.999), and their average recoveries varied between 98.2% and 101.2%. The results indicated that this method was simple, effective and suitable for the quality control of GFC.
authors.affiliation: a School of Pharmacy, Shenyang Pharmaceutical University , Shenyang , Liaoning Province , P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sui
authors.affiliation: a School of Pharmacy, Shenyang Pharmaceutical University , Shenyang , Liaoning Province , P.R. China.
authors.forename: Long-Shan
authors.initials: LS
authors.lastname: Zhao
authors.affiliation: b Jiangsu Kanion Pharmaceutical Co. Ltd. , Lianyungang , Jiangsu Province , P.R. China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: a School of Pharmacy, Shenyang Pharmaceutical University , Shenyang , Liaoning Province , P.R. China.
authors.forename: Yu-Tong
authors.initials: YT
authors.lastname: Zhao
authors.affiliation: b Jiangsu Kanion Pharmaceutical Co. Ltd. , Lianyungang , Jiangsu Province , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: a School of Pharmacy, Shenyang Pharmaceutical University , Shenyang , Liaoning Province , P.R. China.
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Xiong
mesh_terms: D000098:Acetophenones / Q000032:analysis; D000678:Amygdalin / Q000032:analysis; D001952:Bridged-Ring Compounds / Q000032:analysis; D002214:Capsules; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D008872:Microwaves; D039821:Monoterpenes / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D014315:Triterpenes / Q000032:analysis; D006027:Glycosides
chemical_list: D000098:Acetophenones; D000678:Amygdalin; D001952:Bridged-Ring Compounds; D002214:Capsules; D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D005707:Gallic Acid; D005960:Glucosides; D039821:Monoterpenes; D014315:Triterpenes; C525079:guizhi-fuling; C029010:cinnamic acid; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C013638:paeonol; C102487:pachymic acid
keywords: GFC; MAE; UPLC-MS/MS; quantitative analysis