title: Tumor-suppressive microRNA-497 targets IKKβ to regulate NF-κB signaling pathway in human prostate cancer cells.
abstract: BACKGROUND
  Prostate cancer (PCa) is one of the most prevalent malignant tumors, PCa-related death is mainly due to the high probability of metastasis. MicroRNAs (miRNAs) play an important role in cancer initiation, progression and metastasis by regulating their target genes.
  
  
  METHODS
  real-time PCR was used to detected the expression of microRNA-497. The molecular biological function was investigated by using cell proliferation assays, cell cycle assay, and migration and invasion assay. We used several Algorithms and confirmed that IKKβ is directly regulated by miR-497.
  
  
  RESULTS
  Here, we found miR-497 is downregulated in human prostate cancer (PCa) and inhibites the proliferation activity, migration and invasion of PC3-AR cells. Subsequently, IKKβ is confi rmed as a target of miR-497. Furthermore, knockdown of IKKβ expression resulted in decreased proliferation activity, migration and invasion. Finally, similar results was found after treatment with a novel IKK-β inhibitor (IMD-0354) in PC3-AR cells. CDK8, MMP-9, and PSA were involved in all these process.
  
  
  CONCLUSIONS
  Taken together, our results show evidence that miR-497 may function as a tumor suppressor genes by regulating IKK-β in PCa, and may provide a strategy for blocking PCa metastasis.
authors.affiliation: Department of Urology, Xinhua Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Xiang-Jie
authors.initials: XJ
authors.lastname: Kong
authors.affiliation: Department of Urology, Xinhua Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Liu-Jian
authors.initials: LJ
authors.lastname: Duan
authors.affiliation: Department of Urology, Xinhua Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Qian
authors.affiliation: Department of Urology, Xinhua Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Urology, Xinhua Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Liu
authors.affiliation: Department of Urology, Shanghai First People's Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Ying-Jian
authors.initials: YJ
authors.lastname: Zhu
authors.affiliation: Department of Urology, Xinhua Hospital, Shanghai Jiaotong University Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
keywords: IKKβ; IMD-0354; MiR-497; PCa; invasion; migration