title: High-throughput screening of cellular redox sensors using modern redox proteomics approaches.
abstract: Cancer cells are characterized by higher levels of intracellular reactive oxygen species (ROS) due to metabolic aberrations. ROS are widely accepted as second messengers triggering pivotal signaling pathways involved in the process of cell metabolism, cell cycle, apoptosis, and autophagy. However, the underlying cellular mechanisms remain largely unknown. Recently, accumulating evidence has demonstrated that ROS initiate redox signaling through direct oxidative modification of the cysteines of key redox-sensitive proteins (termed redox sensors). Uncovering the functional changes underlying redox regulation of redox sensors is urgently required, and the role of different redox sensors in distinct disease states still remains to be identified. To assist this, redox proteomics has been developed for the high-throughput screening of redox sensors, which will benefit the development of novel therapeutic strategies for cancer treatment. Highlighted here are recent advances in redox proteomics approaches and their applications in identifying redox sensors involved in tumor development.
authors.affiliation: a 1 State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, PR China.|b 2 Hainan Medical University, Haikou, 571199, PR China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Jiang
authors.affiliation: a 1 State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Wang
authors.affiliation: c 3 Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Edouard C
authors.initials: EC
authors.lastname: Nice
authors.affiliation: d 4 School of Biomedical Sciences, Chengdu Medical College, Chengdu 610500, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: a 1 State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, PR China.|b 2 Hainan Medical University, Haikou, 571199, PR China.
authors.forename: Canhua
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D010084:Oxidation-Reduction; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D020543:Proteome; D017382:Reactive Oxygen Species
keywords: Biotin switch; Thiol-trapping; mass spectrometry; reactive oxygen species; redox proteomics