title: A new sesquiterpene from the entomogenous fungus Phomopsis amygdali.
abstract: A new sesquiterpene, (+)-S-1-methyl-abscisic-6-acid (1), together with five known compounds, (+)-S-abscisic acid (2), fusicoccin J (3), 3α-hydroxyfusicoccin J (4), (R)-5-hydroxymethylmellein (5) and 4-hydroxyphenethyl acetate (6) was isolated from the fermentation extract of Phomopsis amygdali, an entomogenous fungus isolated from Call midge. Their structures were determined mainly by analysis of MS and NMR spectroscopic data. Compounds 1-6 were tested for antimicrobial activity against three plant pathogenic fungi: Gibberella zeae, Verticillium albo-atrum, and Fusarium nivale, and two bacteria: Escherichia coli and Pseudomonas aeruginosa 2033E. As a result, compounds 1-4 displayed antibacterial activity against Gram-negative P. aeruginosa 2033E, and the minimum inhibition concentration (MIC value) of 1-4 is 30 μg/mL, 58 μg/mL, 26 μg/mL, and 26 μg/mL, respectively.
authors.affiliation: a College of Life and Environmental Sciences, Minzu University of China , Beijing 100081 , P.R. China.|b State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ma
authors.affiliation: a College of Life and Environmental Sciences, Minzu University of China , Beijing 100081 , P.R. China.|c Beijing Engineering Research Center of Food Environment and Health, Minzu University of China , Beijing 100081 , P.R. China.
authors.forename: Wenshu
authors.initials: W
authors.lastname: Wang
authors.affiliation: b State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , P.R. China.
authors.forename: Erwei
authors.initials: E
authors.lastname: Li
authors.affiliation: a College of Life and Environmental Sciences, Minzu University of China , Beijing 100081 , P.R. China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Gao
authors.affiliation: b State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , P.R. China.
authors.forename: Liangdong
authors.initials: L
authors.lastname: Guo
authors.affiliation: b State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , P.R. China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Pei
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D002683:Chironomidae / Q000382:microbiology; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010935:Plant Diseases / Q000382:microbiology; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012717:Sesquiterpenes
keywords: (+)-S-1-methyl-abscisic-6-acid; Phomopsis amygdali; Pseudomonas aeruginosa 2033; antimicrobial activity; entomogenous fungus