title: Six new metabolites produced by Colletotrichum aotearoa 09F0161, an endophytic fungus isolated from Bredia oldhamii.
abstract: Six new compounds, colletobredins A-D (1-4) and colletomelleins A and B (5 and 6), along with 12 previously identified compounds, were isolated from the culture broth of Colletotrichum aotearoa BCRC 09F0161, a fungal endophyte residing in the leaves of an endemic Formosan plant Bredia oldhamii Hook. f. (Melastomataceae). The structures of the new compounds were established by spectroscopic methods, including UV, IR, HR-ESIMS and extensive 1D and 2D NMR techniques. The effects of some isolates on the inhibition of nitric oxide (NO) production in lipopolysaccharide-activated murine macrophage RAW264.7 cells were evaluated. All these compounds inhibited NO production in activated macrophages without any cytotoxicity at a concentration of 100 μM. Of these isolates, 1 showed weak NO inhibitory activity with IC50 value of 182.2 μM. To the best of our knowledge, this is the first report on isochroman glycoside metabolites (1-4) from the genus Colletotrichum.
authors.affiliation: a College of Pharmacy, Graduate Institute of Natural Products, Kaohsiung Medical University , Kaohsiung 807 , Taiwan.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hsiao
authors.affiliation: b Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI) , Hsinchu 300 , Taiwan.
authors.forename: Ming-Jen
authors.initials: MJ
authors.lastname: Cheng
authors.affiliation: a College of Pharmacy, Graduate Institute of Natural Products, Kaohsiung Medical University , Kaohsiung 807 , Taiwan.|c School of Pharmacy, College of Pharmacy , Kaohsiung Medical University , Kaohsiung 807 , Taiwan.
authors.forename: Hsun-Shuo
authors.initials: HS
authors.lastname: Chang
authors.affiliation: b Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI) , Hsinchu 300 , Taiwan.
authors.forename: Ming-Der
authors.initials: MD
authors.lastname: Wu
authors.affiliation: b Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI) , Hsinchu 300 , Taiwan.
authors.forename: Sung-Yuan
authors.initials: SY
authors.lastname: Hsieh
authors.affiliation: b Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI) , Hsinchu 300 , Taiwan.
authors.forename: Ta-Wei
authors.initials: TW
authors.lastname: Liu
authors.affiliation: a College of Pharmacy, Graduate Institute of Natural Products, Kaohsiung Medical University , Kaohsiung 807 , Taiwan.|c School of Pharmacy, College of Pharmacy , Kaohsiung Medical University , Kaohsiung 807 , Taiwan.
authors.forename: Chu-Hung
authors.initials: CH
authors.lastname: Lin
authors.affiliation: b Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI) , Hsinchu 300 , Taiwan.
authors.forename: Gwo-Fang
authors.initials: GF
authors.lastname: Yuan
authors.affiliation: a College of Pharmacy, Graduate Institute of Natural Products, Kaohsiung Medical University , Kaohsiung 807 , Taiwan.|c School of Pharmacy, College of Pharmacy , Kaohsiung Medical University , Kaohsiung 807 , Taiwan.
authors.forename: Ih-Sheng
authors.initials: IS
authors.lastname: Chen
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020231:Colletotrichum / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D035322:Melastomataceae / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D018515:Plant Leaves / Q000382:microbiology; D000067996:RAW 264.7 Cells; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide
keywords: Colletotrichum aotearoa; isochroman; leaves