title: Differential identification of mushrooms sclerotia by IR macro-fingerprint method.
abstract: Many macrofungus sclerotia are well-known medicinal herbs, health food and nutritional supplements. However, the prevalent adulterant commercial products are major hindrances to their incorporation into mainstream medical use in many countries. The mushroom sclerotia of Lignosus rhinocerotis, Poria cocos, Polyporus umbellatus, Pleurotus tuber-regium and Omphalia lapidescens are commonly used in traditional Chinese medicine. In this study, IR macro-fingerprint method was used in the identification of these sclerotia. The results showed that the spectrum of L. rhinocerotis (LR) was comparable with P. cocos with 94.4% correlation, except that the peak at 1543cm(-1) of LR appeared in lower intensity. The spectrum of P. umbellatus and P. tuber-regium was also correlated (91.5%), as both spectra could be clearly discriminated in that P. umbellatus spectrum has small base peaks located at the range of 1680-1500cm(-1). O. lapidescens was not comparable with all the other sclerotia as its spectrum was totally different. Its base peak was broad and derivated equally along the range. The first IR has revealed the dissimilarity among five mushrooms sclerotia. The second derivative and 2DIR further enhanced the identification in detail.
authors.affiliation: Phytochemistry Unit, Herbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Electronic address: yewkeong@imr.gov.my.
authors.forename: Yew Keong
authors.initials: YK
authors.lastname: Choong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lan
authors.affiliation: Medical Entomology Unit, WHO Collaborating Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
authors.forename: Han Lim
authors.initials: HL
authors.lastname: Lee
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. Electronic address: xdchen@126.com.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Wang
authors.affiliation: National Research Institute for Family Planning of China, Beijing 100081, PR China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Yang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D020076:Pleurotus / Q000737:chemistry; D055492:Polyporus / Q000737:chemistry; D055441:Poria / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
keywords: Fourier transform infrared spectroscopy; Identification; Mushrooms sclerotia; Two-dimensional correlation infrared spectroscopy