title: MicroRNA-495 downregulates FOXC1 expression to suppress cell growth and migration in endometrial cancer.
abstract: MicroRNAs (miRNAs) are a class of noncoding RNAs and function as key regulators of gene expression at the post-transcriptional level. In this study, we found that miR-495 reduces cell growth, induces apoptosis and suppresses the migration of endometrial cancer by directly inhibiting FOXC1 expression. Further analysis revealed that FOXC1 promotes growth and migration and functions as an oncogene in vitro. FOXC1 overexpression reversed the cellular responses mediated by miR-495 in endometrial cancer cells. We also found that miR-495 suppresses the growth of endometrial cancer in vivo. Altogether, these results indicate that miR-495 acts as a tumour suppressor gene by targeting FOXC1 at the post-transcriptional level in endometrial cancer.
authors.affiliation: Department of Gynecology, The Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Xu
authors.affiliation: National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Department of Gynecological Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Department of Gynecological Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China. quanhao2002@163.com.
authors.forename: Quan
authors.initials: Q
authors.lastname: Hao
authors.affiliation: Department of Gynecology, The Second Hospital of Tianjin Medical University, Tianjin, China. yinlr2012@163.com.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Yin
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D009857:Oncogenes; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C112743:FOXC1 protein, human; D051858:Forkhead Transcription Factors; C577768:MIRN495 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins
keywords: Apoptosis; Endometrial cancer; FOXC1; MicroRNA; Oncogene