title: The molecular basis for flexibility in the flexible filamentous plant viruses.
abstract: Flexible filamentous plant viruses cause more than half the viral crop damage in the world but are also potentially useful for biotechnology. Structural studies began more than 75 years ago but have failed, owing to the virion's extreme flexibility. We have used cryo-EM to generate an atomic model for bamboo mosaic virus, which reveals flexible N- and C-terminal extensions that allow deformation while still maintaining structural integrity.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: DiMaio
authors.affiliation: 1] Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. [2] Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan. [3] Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Chun-Chieh
authors.identifier: 0000000347992302
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Xiong
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Frenz
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Yau-Heiu
authors.initials: YH
authors.lastname: Hsu
authors.affiliation: 1] Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. [2] Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan. [3] Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Na-Sheng
authors.initials: NS
authors.lastname: Lin
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Edward H
authors.initials: EH
authors.lastname: Egelman
mesh_terms: D031723:Bambusa / Q000821:virology*; D002213:Capsid / Q000737:chemistry / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D009029:Mosaic Viruses / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral