title: Suppression of miR-221 inhibits glioma cells proliferation and invasion via targeting SEMA3B.
abstract: BACKGROUND
  Gliomas are the most common primary tumors in the central nervous system. Due to complicated signaling pathways involved in glioma progression, effective targets for treatment and biomarkers for prognosis prediction are still scant.
  
  
  RESULTS
  In this study we revealed that a new microRNA (miR), the miR-221, was highly expressed in the glioma cells, and suppression of miR-221 resulted in decreased cellular proliferation, migration, and invasion in glioma cells. Mechanistic experiments validated that miR-221 participates in regulating glioma cells proliferation and invasion via suppression of a direct target gene, the Semaphorin 3B (SEMA3B). The rescue experiment with miR-221 and SEMA3B both knockdown results in significant reversion of miR-221 induced phenotypes.
  
  
  CONCLUSIONS
  Taken together, our findings highlight an unappreciated role for miR-221 and SEMA3B in glioma.
authors.affiliation: Department of Neurology, Beijing Friendship Hospital, Capital Medical University, 95 Yong'an Rd, Xicheng, Beijing, 100050, China. caiguilan_0416@163.com.
authors.forename: Guilan
authors.initials: G
authors.lastname: Cai
authors.affiliation: Department of Neurology, Beijing Friendship Hospital, Capital Medical University, 95 Yong'an Rd, Xicheng, Beijing, 100050, China. zichenwang2015@163.com.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Qiao
authors.affiliation: Department of Neurology, Beijing Friendship Hospital, Capital Medical University, 95 Yong'an Rd, Xicheng, Beijing, 100050, China. chenkui2002@163.com.
authors.forename: Kui
authors.initials: K
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000494:pharmacology*; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D060888:Real-Time Polymerase Chain Reaction; D039961:Semaphorins / Q000235:genetics / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D008156:Luciferases; D008562:Membrane Glycoproteins; D035683:MicroRNAs; D039961:Semaphorins; C525709:MIR221, human; C409766:SEMA3B protein, human