title: Mesoscale infraslow spontaneous membrane potential fluctuations recapitulate high-frequency activity cortical motifs.
abstract: Neuroimaging of spontaneous, resting-state infraslow (<0.1 Hz) brain activity has been used to reveal the regional functional organization of the brain and may lead to the identification of novel biomarkers of neurological disease. However, these imaging studies generally rely on indirect measures of neuronal activity and the nature of the neuronal activity correlate remains unclear. Here we show, using wide-field, voltage-sensitive dye imaging, the mesoscale spatiotemporal structure and pharmacological dependence of spontaneous, infraslow cortical activity in anaesthetized and awake mice. Spontaneous infraslow activity is regionally distinct, correlates with electroencephalography and local field potential recordings, and shows bilateral symmetry between cortical hemispheres. Infraslow activity is attenuated and its functional structure abolished after treatment with voltage-gated sodium channel and glutamate receptor antagonists. Correlation analysis reveals patterns of infraslow regional connectivity that are analogous to cortical motifs observed from higher-frequency spontaneous activity and reflect the underlying framework of intracortical axonal projections.
authors.affiliation: 1] Department of Psychiatry, University of British Columbia, Detwiller Pavilion, Room 4842, Vancouver, British Columbia, Canada V6T 2A1 [2] Brain Research Centre, University of British Columbia, Room F117, 2211 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 2B5 [3] Department of Medicine and Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 1M9, Canada.
authors.forename: Allen W
authors.initials: AW
authors.lastname: Chan
authors.affiliation: 1] Department of Psychiatry, University of British Columbia, Detwiller Pavilion, Room 4842, Vancouver, British Columbia, Canada V6T 2A1 [2] Brain Research Centre, University of British Columbia, Room F117, 2211 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 2B5.
authors.forename: Majid H
authors.initials: MH
authors.lastname: Mohajerani
authors.affiliation: 1] Department of Psychiatry, University of British Columbia, Detwiller Pavilion, Room 4842, Vancouver, British Columbia, Canada V6T 2A1 [2] Brain Research Centre, University of British Columbia, Room F117, 2211 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 2B5.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: LeDue
authors.affiliation: 1] Brain Research Centre, University of British Columbia, Room F117, 2211 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 2B5 [2] Department of Medicine and Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 1M9, Canada [3] Translational Medicine Research Center, China Medical University, Taichung 40402, Taiwan.
authors.forename: Yu Tian
authors.initials: YT
authors.lastname: Wang
authors.affiliation: 1] Department of Psychiatry, University of British Columbia, Detwiller Pavilion, Room 4842, Vancouver, British Columbia, Canada V6T 2A1 [2] Brain Research Centre, University of British Columbia, Room F117, 2211 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 2B5 [3] Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001369:Axons; D001931:Brain Mapping; D058256:Brain Waves / Q000187:drug effects / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D004569:Electroencephalography; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010812:Physical Stimulation; D062211:Spatio-Temporal Analysis; D061567:Voltage-Gated Sodium Channel Blockers / Q000494:pharmacology; D056969:Voltage-Sensitive Dye Imaging; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology
chemical_list: D018691:Excitatory Amino Acid Antagonists; D061567:Voltage-Gated Sodium Channel Blockers