title: Exposure to Aroclor-1254 impairs spindle assembly during mouse oocyte maturation.
abstract: Polychlorinated biphenyls (PCBs), as typical environmental estrogen disruptors, are a structurally-related group of halogenated aromatic hydrocarbons that are composed of 209 isomers and present as a mixture in the environment. PCBs congener with different numbers and positions of chlorine atoms substituted on the biphenyl moiety. Aroclor-1254 is a mixture of more than 60 PCB congeners. Previous studies have provided the evidence that PCBs have severe negative effects on reproductive functions, but the effects of PCBs on spindle assembly during mouse oocyte maturation in vitro have not been reported. In the present study, female ICR mouse immature oocytes were cultured in M2 medium with 1 and 10 μg mL-1Aroclor-1254 separately in vitro. The percentage of germinal vesicle breakdown (GVBD) and the first polar body extrusion were recorded. The results showed no significant difference in the percentage of GVBD or the first polar body extrusion between control oocytes and Aroclor-1254-treated oocytes. Further studies showed that the normal localization of γ-tubulin and Aurora-A kinase was interfered and α-tubulin assembling into spindle was affected when mouse oocytes were exposed to Aroclor-1254. The length of spindle from 10 μg mL-1Aroclor-1254-treated oocytes was longer than that from control oocytes, and the spindle area in the Aroclor-1254-treated groups were decreased. Furthermore, the percentage of DNA damage in cumulus cells revealed an increase after exposed to Aroclor-1254. These results will provide the important reference for the prevention of reproductive disorders caused by PCBs. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1652-1662, 2016.
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China. shuzhen26@163.com.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Liu
authors.affiliation: Affiliated Hospital of Jining Medical University, Jining, China.
authors.forename: Ze-Feng
authors.initials: ZF
authors.lastname: Wei
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: Xiao-Qian
authors.initials: XQ
authors.lastname: Meng
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Han
authors.affiliation: Department of Toxicology, Shandong Center for Disease Control and Prevention, Jinan, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.affiliation: Department of Toxicology, Shandong Center for Disease Control and Prevention, Jinan, China.
authors.forename: Tian-Liang
authors.initials: TL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Wang
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects*; D008941:Spindle Apparatus / Q000187:drug effects*
chemical_list: D020111:Chlorodiphenyl (54% Chlorine)
keywords: Aroclor-1254; Aurora-A; mouse; oocyte; spindle; γ-tubulin