title: Effect of hyperthermic CO2-treated dendritic cell-derived exosomes on the human gastric cancer AGS cell line.
abstract: The aim of the present study was to determine the antitumor effects of hyperthermic CO2(HT-CO2)-treated dendritic cell (DC)-derived exosomes (Dex) on human gastric cancer AGS cells. Mouse-derived DCs were incubated in HT-CO2at 43°C for 4 h. The exosomes in the cell culture supernatant were then isolated. Cell proliferation was analyzed using the cell counting kit-8 (CCK-8) assay. Cell apoptosis was observed using flow cytometry, Hoechst 33258 staining and the analysis of caspase-3 activity. In addition, the proliferation of tumor cells was evaluated in xenotransplant nude mice. HT-CO2markedly inhibited cell proliferation, as assessed by the CCK-8 assay, and also induced apoptosis in a time-dependent manner, as demonstrated by Annexin V/propidium iodide flow cytometry, caspase-3 activity and morphological analysis using Hoechst fluorescent dye. It was also revealed that HT-CO2-treated Dex decreased the expression of heat shock protein 70 and inhibited tumor growth in nude mice. In conclusion, HT-CO2exerted an efficacious immune-enhancing effect on DCs. These findings may provide a novel strategy for the elimination of free cancer cells during laparoscopic resection. However, the potential cellular mechanisms underlying this process require further investigation.
authors.affiliation: Department of General Surgery, Dongguan People's Hospital, Dongguan, Guangdong 523059, P.R. China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Dongguan People's Hospital, Dongguan, Guangdong 523059, P.R. China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Mo
authors.affiliation: Department of General Surgery, Dongguan People's Hospital, Dongguan, Guangdong 523059, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wei
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
keywords: apoptosis antitumor effect; dendritic cell; dendritic cell-derived exosomes; gastric adenocarcinoma; heat-CO2 pneumoperitoneum; proliferation