title: [Detection of the BCR-ABL fusion: Comparative analysis of six commercially available FISH fusion probes].
abstract: Chronic myeloid leukemia (CML) is diagnostically defined by the reciprocal translocation t(9;22)(q34;q11). This aberration can be detected by the BCR-ABL fluorescence in situ hybridization (FISH) technique. This article presents a comparative analysis of different commercially available FISH probes and different FISH protocols in order to optimize this technique on formalin-fixed and paraffin-embedded bone marrow trephine biopsies.
authors.affiliation: Institut für Pathologie, Universitätsklinikum Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Deutschland.
authors.forename: T
authors.initials: T
authors.lastname: Knas
authors.forename: M
authors.initials: M
authors.lastname: Buck
authors.forename: F
authors.initials: F
authors.lastname: Stegelmann
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.forename: T F
authors.initials: TF
authors.lastname: Barth
mesh_terms: D001707:Biopsy, Needle; D001853:Bone Marrow / Q000473:pathology*; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D016044:Fusion Proteins, bcr-abl / Q000032:analysis* / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology*; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic
chemical_list: D016044:Fusion Proteins, bcr-abl