title: Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp (Ctenopharyngodon idella).
abstract: The ontogeny and kinetics of carnitine palmitoyltransferase I (CPT I) were investigated in hepatopancreas and muscle throughout four developmental stages (newly hatched larvae, 1-month-old juvenile, 3-month-old, and 6-month-old, respectively) of grass carp Ctenopharyngodon idella. In hepatopancreas, the maximal velocity (Vmax) significantly increased from hatching to 1-month-old grass carp and then gradually declined at 6-month-old grass carp. In muscle, CPT I activity was the highest at 1-month-old grass carp, nearly twofold higher than that at hatching (P < 0.05). The Michaelis constant (Km) value was also the highest for 1-month-old in both tested tissues. Carnitine concentrations (FC, AC and TC) were the lowest for 3-month-old grass carp and remained relatively constant in both tissues from fish under the other developmental stages. The FC concentration in hepatopancreas and muscle at four developmental stages were less than the respective Km, indicating that grass carp required supplemental carnitine in their food to ensure that CPT I activity was not constrained by carnitine availability.
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. luozhi99@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, People's Republic of China. luozhi99@mail.hzau.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Luo
authors.affiliation: College of Fisheries, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Kang-Sen
authors.initials: KS
authors.lastname: Mai
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, People's Republic of China.|Bureau of Aquatic Products of Xiantao City, Xiantao, 433000, People's Republic of China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Liu
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, People's Republic of China.|National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002331:Carnitine / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D043143:Hepatopancreas / Q000201:enzymology*; D007700:Kinetics; D007814:Larva / Q000378:metabolism; D050356:Lipid Metabolism; D018482:Muscle, Skeletal / Q000201:enzymology*
chemical_list: D002334:Carnitine O-Palmitoyltransferase; D002331:Carnitine
keywords: CPT I; Carnitine; Ctenopharyngodon idella; Development; Kinetics