title: [Intracranial aneurysms and their clinical and genetic behaviour].
abstract: BACKGROUND
  Intracranial aneurysms are abnormal dilations of the cerebral arteries of unknown origin. However, some genes have been linked to their formation, as in the case of NOS3 gene which encodes the endothelial nitric oxide synthase responsible for producing nitric oxide. Several polymorphisms in this gene, in association with a variable number tandem repeat located in intron 4 from eNOS4 gene, can influence the formation of aneurysms. Therefore, the purpose of this study is to determine the genotype frequencies of eNOS3 and eNOS4 genes, and their relationship with intracranial aneurysms.
  
  
  METHODS
  A prospective case-control study was performed on 79 cases with ruptured intracranial aneurysm and 93 healthy controls. DNA was obtained from all subjects for the study of the eNOS3 and eNOS4 genes by molecular techniques.
  
  
  RESULTS
  The GG genotype of eNOS3 gene showed the largest number of patients (n=29) with a large aneurysm. While the intracranial aneurysms of medium size were found in a higher percentage (50%) in patients with genotype GT. In terms of patient outcomes, it was observed that those with genotype GG had the highest percentage (43.13%) recovery, compared to genotype GT (27.27%).
  
  
  CONCLUSIONS
  The present study shows that there is a tendency of an association between genotypes of eNOS3 gene with the mean size of the aneurysm, as well as clinical sequelae of the disease in patients with intracranial aneurysms.
authors.affiliation: División de Medicina Genómica, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., México. Electronic address: garortiz@yahoo.com.
authors.forename: Liliana
authors.initials: L
authors.lastname: García-Ortiz
authors.affiliation: Laboratorio de Bioquímica y Medicina Experimental, División de Investigación Biomédica, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., México.
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez-Salinas
authors.affiliation: Cirugía Cerebro Vascular, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., México.
authors.forename: Salvador
authors.initials: S
authors.lastname: Guerrero-Muñiz
authors.affiliation: División de Medicina Genómica, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., México.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Chima-Galán
authors.affiliation: Licenciatura en Química, Facultad de Estudios Superiores Cuautitlán-Universidad Nacional Autónoma de México, Cuautitlán, Estado de México, México.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sánchez-Hernández
mesh_terms: D000328:Adult; D000368:Aged; D017542:Aneurysm, Ruptured / Q000235:genetics; D000886:Anthropometry; D001925:Brain Damage, Chronic / Q000209:etiology / Q000235:genetics; D016022:Case-Control Studies; D002536:Cerebral Arteries / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D002532:Intracranial Aneurysm / Q000150:complications / Q000235:genetics* / Q000473:pathology; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012307:Risk Factors
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Aneurismas intracraneales; Características clínicas; Clinical features; Endotelial nitric oxide synthase; Intracranial aneurysms; Número variable de repeticiones en tándem; Polimorfismos; Polymorphisms; Sintetasa de óxido nítrico endotelial; Variable number tandem repeat