title: Interactions of zearalenone with native and chemically modified cyclodextrins and their potential utilization.
abstract: Zearalenone (ZEA) is a widespread xenoestrogenic mycotoxin produced by several Fusarium species. ZEA can cause reproductive disorders in farm animals and hyperoestrogenic syndromes in humans; therefore, development of more sensitive analytical methods (to quantify the mycotoxin) as well as strategies for prevention of its toxic impacts is highly important. In this study, the interactions of ZEA with native and chemically modified cyclodextrins (CDs) were investigated using fluorescence spectroscopy. Furthermore, in vitro experiments on liver cells were also performed to test the potential effect of CDs on toxin uptake. Our results demonstrate that ZEA forms stable complexes with CDs (logK values are approximately 3.7-4.7) resulting in the considerable elevation of its fluorescence signal. In addition, some of the CDs show ability to inhibit the cellular uptake of ZEA, suggesting their potential suitability to develop new CD-based preventive/detoxification strategies against ZEA in the future.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Toxicology Section, University of Pécs, Szigeti út 12, Pécs H-7624, Hungary. Electronic address: poor.miklos@pte.hu.
authors.forename: Miklós
authors.initials: M
authors.lastname: Poór
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6, Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20, Pécs H-7624, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Kunsági-Máté
authors.affiliation: János Szentágothai Research Center, Ifjúság útja 20, Pécs H-7624, Hungary; Institute of Laboratory Medicine, University of Pécs, Ifjúság útja 13, Pécs H-7624, Hungary.
authors.forename: Nikolett
authors.initials: N
authors.lastname: Sali
authors.affiliation: János Szentágothai Research Center, Ifjúság útja 20, Pécs H-7624, Hungary; Institute of Laboratory Medicine, University of Pécs, Ifjúság útja 13, Pécs H-7624, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Kőszegi
authors.affiliation: CycloLab Cyclodextrin Research & Development Laboratory, Ltd., Illatos út 7, Budapest H-1097, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Szente
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6, Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20, Pécs H-7624, Hungary.
authors.forename: Beáta
authors.initials: B
authors.lastname: Peles-Lemli
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D005453:Fluorescence; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D000432:Methanol / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D015025:Zearalenone / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; D015025:Zearalenone; D000432:Methanol; C032159:acetonitrile
keywords: Cyclodextrin; Detoxification; Fluorescence enhancement; Fluorescence spectroscopy; Zearalenone