title: Dynamics of brominated flame retardants removal in contaminated wastewater sewage sludge under anaerobic conditions.
abstract: Disposal of solid waste to landfills from waste water sewage treatment plants (WWTPs) serves as a potential source of contamination by polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD). Native microbial communities have been found to degrade a variety of xenobiotics, such as PBDEs and HBCDs. This study investigates the potential of autochthonous microflora to remove 11 PBDE congeners and HBCDs in waste water sludge under anaerobic conditions. Laboratory microcosms were constructed with sewage sludge from the WWTPs of Hradec Kralove and Brno. BDE 209 was detected as the prevailing congener in concentrations 685 and 1403 ng/g dw and the total amounts of 10 lower PBDEs (BDE 28, 47, 49, 66, 85, 99, 100, 153, 154, 183) were 605 and 205 ng/g dw in sludge from Hradec Kralove and Brno, respectively. The levels of HBCD were detected in both sludge lower than 24 ng/g dw. The experiment was carried out for 15 months. After three months of incubation, HBCD was completely degraded to below detection limits. In sewage from both WWTPs, the higher brominated DEs were removed faster than the lower brominated congeners. One exception was tri-BDE, which was degraded completely within 15 months of cultivation. A significant increase in congener tetra-BDE 49 concentrations was observed over the course of the experiment in all tested sewage. The relative distribution of individual congeners among all PBDEs changed after 15 months of the incubation in favour of lower brominated congeners. This indicates that debromination is the major mechanism of anaerobic biodegradation. Despite of the increase of BDE 49, the overall removal of all 11 PBDEs achieved the levels of 47.4 and 68.7% in samples from WWTPs Hradec Kralove and Brno, respectively.
authors.affiliation: UCT Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague 6, Czech Republic. Electronic address: hana.stiborova@vscht.cz.
authors.forename: Hana
authors.initials: H
authors.lastname: Stiborova
authors.affiliation: UCT Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague 6, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Vrkoslavova
authors.affiliation: UCT Prague, Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, Technicka 3, 16628 Prague 6, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Pulkrabova
authors.affiliation: UCT Prague, Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, Technicka 3, 16628 Prague 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Poustka
authors.affiliation: UCT Prague, Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, Technicka 3, 16628 Prague 6, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Hajslova
authors.affiliation: UCT Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague 6, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Demnerova
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D005411:Flame Retardants / Q000032:analysis*; D012722:Sewage; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D005411:Flame Retardants; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Anaerobic degradation; Hexabromocyclododecane; Polybrominated diphenyl ethers; Sewage sludge