title: Conjugative Transfer in Staphylococcus aureus.
abstract: The acquisition of plasmids has led to a significant increase in antimicrobial resistance within the staphylococci. In order to study these plasmids effectively, one must be able move the plasmid DNA into genetically clean backgrounds. While the smaller staphylococcal class I (1-5 kb) and class II (10-30 kb) plasmids are readily transferred using bacteriophage transduction or electroporation, these methods are inefficient at moving the larger class III (30-60 kb) plasmids. This review describes methods to transfer class III plasmids via conjugative mobilization.
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, 985900 Nebraska Medical Center, Omaha, NE, 68198-5900, USA.
authors.forename: Cortney R
authors.initials: CR
authors.lastname: Halsey
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, 985900 Nebraska Medical Center, Omaha, NE, 68198-5900, USA. pfey@unmc.edu.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Fey
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D013203:Staphylococcal Infections / Q000235:genetics / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial
keywords: Conjugation; Plasmid; Staphylococci