title: Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism.
abstract: Aeromonas molluscorum Av27 cells were exposed to 0, 5 and 50 μM of TBT and the respective transcriptomes were obtained by pyrosequencing. Gene Ontology revealed that exposure to 5 μM TBT results in a higher number of repressed genes in contrast with 50 μM of TBT, where the number of over-expressed genes is greater. At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction. A number of proteins are affected by TBT, such as the acriflavin resistance protein, several transcription-related proteins, several Hsps, ABC transporters, CorA and ZntB and other outer membrane efflux proteins, all of these involved in cellular metabolic processes, important to maintain overall cell viability. Using the STRING tool, several proteins with unknown function were related with others involved in degradation processes, such as the pyoverdine chromophore biosynthetic protein, that has been described as playing a role in the Sn-C cleavage of organotins. This approach has allowed a better understanding of the molecular effects of exposure of bacterial cells to TBT. Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant. Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.
authors.affiliation: Biology Department & CESAM, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal. Electronic address: andreia.cruz@ua.pt.
authors.forename: Andreia
authors.initials: A
authors.lastname: Cruz
authors.affiliation: Biology Department & CESAM, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodrigues
authors.affiliation: Advanced Services Unit, Biocant - Biotechnology Innovation Center, 3060-325, Cantanhede, Portugal; School of Medicine, University of St. Andrews, North Haugh, KY16 9TF, St. Andrews, UK.
authors.forename: Miguel
authors.initials: M
authors.lastname: Pinheiro
authors.affiliation: Biology Department & CESAM, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
authors.forename: Sónia
authors.initials: S
authors.lastname: Mendo
mesh_terms: D000333:Aeromonas / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial / Q000187:drug effects*; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin
keywords: Acriflavine; Dioxygenase; Pyoverdine; Quantitative real-time PCR; Tributyltin