title: Hydrogen deuterium mass spectrometry in drug discovery.
abstract: Hydrogen deuterium exchange mass spectrometry (HDX-MS) has become an increasingly routine technology for probing the solution structure and dynamics of proteins. HDX-MS measures the exchange of deuterium with the amide proton on the protein backbone chain. HDX-MS results can be used to aid in construct design for biophysical studies, to probe protein-ligand interactions and to characterize the dynamics of proteins. This digest highlights recent progress which makes this technology accessible and explores examples of HDX-MS in drug discovery and solution state structural biology.
authors.affiliation: GlaxoSmithKline R&D, Molecular Discovery Research, US-Biophysics, Collegeville, PA 19426, USA. Electronic address: nino.2.campobasso@gsk.com.
authors.forename: Nino
authors.initials: N
authors.lastname: Campobasso
authors.affiliation: GlaxoSmithKline R&D, Molecular Discovery Research, US-Biophysics, Collegeville, PA 19426, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Huddler
mesh_terms: D041961:Deuterium Exchange Measurement; D055808:Drug Discovery / Q000379:methods*; D013058:Mass Spectrometry
keywords: Hydrogen–deuterium exchange mass spectrometry; Structural biology