title: [Morphological Characteristics in SD-OCT and Fundus Autofluorescence in Different Ischaemic Retinopathies - Characterisation of a New, Temporary Hyperreflective Band].
abstract: BACKGROUND
  The aim of this study was to investigate alterations in the neurosensory retinal morphology secondary to acute retinal ischaemic conditions. The observations were documented by spectral domain optical coherence tomography (SD-OCT, Heidelberg Engineering) and fundus autofluorescence (FAF) imaging. In addition, the occurrence of an additional hyperrefelctive band in the outer corneal layers is described. This observation was made during the evaluation of OCT images of patients with an acute loss of vision due to retinal ischaemia.
  
  
  METHODS
  SD-OCT images and FAF were used to observe the retinal structure of 26 subjects (36 to 96 years) with acute monocular visual impairment due to central retinal artery occlusion (CRAO), branch retinal artery occlusion (BRAO), anterior ischaemic optic neuropathy (AION) or impending central retinal vein occlusion were included. The main focus of attention was set on the transition of the outer nuclear layer (ONL) to outer plexiform layer (OPL). Furthermore measurements of the retinal thickness at the fovea, parafoveal and within the retinal layers were acquired and compared to corresponding points of the non-affected eyes. SD-OCT images were acquired with a combined SD-OCT and scanning laser ophthalmoscope (cSLO) imaging system using a linear cross hair scan and a 6 line radial scan of 6 mm length (ART mode on; average of 20 frames).
  
  
  RESULTS
  SD-OCT revealed an additional highly reflective band located within the OPL. Morphological characteristics of this hyperdense band were a decreasing intensity with distance from the fovea, partially segmental occurrence and manifestation limited in time. FAF showed areas of increased and decreased signal intensity within the vessel arcade at the posterior pole. The regions of decreased FAF corresponded to perivenous regions and resemble the "frosted branches" sign seen in fluorescein angiography in patients with ocular ischaemia.
  
  
  CONCLUSIONS
  The additional hyperreflective band observed in SD-OCT could represent a marker for retinal ischaemia in subjects without the presence of a complete vascular occlusion. The mid retinal localisation of the band within the OPL is situated at the locus of transition from retinal to choroidal oxygen supply where oxygen diffusion is weakest. Histopathologically the observed structure could represent activated microglial tissue induced by the hypoxia driven upregulation of inflammatory molecules aimed at ischaemia repair.
authors.affiliation: Augenklinik der TU München, Klinikum rechts der Isar.
authors.forename: N
authors.initials: N
authors.lastname: Feucht
authors.affiliation: Augenklinik der TU München, Klinikum rechts der Isar.
authors.forename: F
authors.initials: F
authors.lastname: Heine
authors.affiliation: Augenklinik der TU München, Klinikum rechts der Isar.
authors.forename: C P
authors.initials: CP
authors.lastname: Lohmann
authors.affiliation: Augenklinik der TU München, Klinikum rechts der Isar.
authors.forename: C
authors.initials: C
authors.lastname: Mayer
authors.affiliation: Augenklinik der TU München, Klinikum rechts der Isar.
authors.forename: M M
authors.initials: MM
authors.lastname: Maier
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005451:Fluorescein Angiography / Q000379:methods*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007511:Ischemia / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012160:Retina / Q000473:pathology*; D012171:Retinal Vessels / Q000473:pathology*; D042262:Retinoscopy / Q000379:methods*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*