title: Phylogenetic Relationship of Necoclí Virus to Other South American Hantaviruses (Bunyaviridae: Hantavirus).
abstract: The results of a previous study suggested that Cherrie's cane rat (Zygodontomys cherriei) is the principal host of Necoclí virus (family Bunyaviridae, genus Hantavirus) in Colombia. Bayesian analyses of complete nucleocapsid protein gene sequences and complete glycoprotein precursor gene sequences in this study confirmed that Necoclí virus is phylogenetically closely related to Maporal virus, which is principally associated with the delicate pygmy rice rat (Oligoryzomys delicatus) in western Venezuela. In pairwise comparisons, nonidentities between the complete amino acid sequence of the nucleocapsid protein of Necoclí virus and the complete amino acid sequences of the nucleocapsid proteins of other hantaviruses were ≥8.7%. Likewise, nonidentities between the complete amino acid sequence of the glycoprotein precursor of Necoclí virus and the complete amino acid sequences of the glycoprotein precursors of other hantaviruses were ≥11.7%. Collectively, the unique association of Necoclí virus with Z. cherriei in Colombia, results of the Bayesian analyses of complete nucleocapsid protein gene sequences and complete glycoprotein precursor gene sequences, and results of the pairwise comparisons of amino acid sequences strongly support the notion that Necoclí virus represents a novel species in the genus Hantavirus. Further work is needed to determine whether Calabazo virus (a hantavirus associated with Z. brevicauda cherriei in Panama) and Necoclí virus are conspecific.
authors.affiliation: 1 Grupo Centauro, Facultad de Ciencias Agrarias, Universidad de Antioquia , Medellín, Antioquia, Colombia .
authors.forename: Carolina
authors.initials: C
authors.lastname: Montoya-Ruiz
authors.affiliation: 2 Department of Pathology, University of Texas Medical Branch , Galveston, Texas.
authors.forename: Maria N B
authors.initials: MN
authors.lastname: Cajimat
authors.affiliation: 2 Department of Pathology, University of Texas Medical Branch , Galveston, Texas.
authors.forename: Mary Louise
authors.initials: ML
authors.lastname: Milazzo
authors.affiliation: 3 Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia , Medellín, Antioquia, Colombia .
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Diaz
authors.affiliation: 1 Grupo Centauro, Facultad de Ciencias Agrarias, Universidad de Antioquia , Medellín, Antioquia, Colombia .
authors.forename: Juan David
authors.initials: JD
authors.lastname: Rodas
authors.affiliation: 2 Department of Pathology, University of Texas Medical Branch , Galveston, Texas.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Valbuena
authors.affiliation: 2 Department of Pathology, University of Texas Medical Branch , Galveston, Texas.
authors.forename: Charles F
authors.initials: CF
authors.lastname: Fulhorst
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D003105:Colombia / Q000453:epidemiology; D003141:Communicable Diseases / Q000453:epidemiology* / Q000821:virology; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000453:epidemiology* / Q000821:virology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D016205:Sigmodontinae / Q000821:virology*; D014687:Venezuela / Q000453:epidemiology
chemical_list: D019590:Nucleocapsid Proteins
keywords: Calabazo virus; Cherrie's cane rat; Colombia; Hantavirus; Hantavirus pulmonary syndrome; Necoclí virus; Zygodontomys brevicauda cherriei; Zygodontomys cherriei