title: Human Dental Pulp Stem Cells via the NF-κB Pathway.
abstract: OBJECTIVE
  Odontogenic differentiation of human dental pulp stem cells (HDPSCs) is regulated by multiple factors and signaling molecules. However, their regulatory mechanisms are not completely understood. In this study, we investigated the role of Zinc finger and BTB domain-containing 20 (ZBTB20) in odontoblastic differentiation of HDPSCs.
  
  
  METHODS
  HDPSCs were obtained from human third molars and ZBTB20 expression was examined by qRT-PCR and western blot. Their osteo/odontogenic differentiation and the involvement of NF-κB pathway were subsequently investigated.
  
  
  RESULTS
  The expression of ZBTB20 is upregulated in a time-dependent manner during odontogenic differentiation of hDPSCs. Inhibition of ZBTB20 reduced osteogenic medium (OM)-induced odontogenic differentiation, reflected in decreased alkaline phosphatase (ALP) activity, mineralized nodule formation and mRNA expression of odonto/osteogenic marker genes. In contrast, overexpression of ZBTB20 enhanced ALP activity, mineralization and the expression of differentiation marker genes. Furthermore, the expression of IκBα was increased by ZBTB20 silencing in HDPSCs, whereas ZBTB20 overexpression decreased IκBα and enhanced nuclear NF-κB p65. Inhibition of the NF-κB pathway significantly suppressed the odontogenic differentiation of HDPSCs induced by ZBTB20.
  
  
  CONCLUSIONS
  This study shows for the first time that ZBTB20 plays an important role during odontoblastic differentiation of HDPSCs and may have clinical implications for regenerative endodontics.
authors.forename: Shensheng
authors.initials: S
authors.lastname: Gu
authors.forename: Shujun
authors.initials: S
authors.lastname: Ran
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Dong
authors.initials: D
authors.lastname: Cao
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Jingping
authors.initials: J
authors.lastname: Liang
mesh_terms: D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D020868:Gene Silencing; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D003470:Culture Media; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C427486:ZBTB20 protein, human; D000469:Alkaline Phosphatase