title: Tyrosine- and tryptophan-coated gold nanoparticles inhibit amyloid aggregation of insulin.
abstract: Here, we have strategically synthesized stable gold (AuNPs(Tyr), AuNPs(Trp)) and silver (AgNPs(Tyr)) nanoparticles which are surface functionalized with either tyrosine or tryptophan residues and have examined their potential to inhibit amyloid aggregation of insulin. Inhibition of both spontaneous and seed-induced aggregation of insulin was observed in the presence of AuNPs(Tyr), AgNPs(Tyr), and AuNPs(Trp) nanoparticles. These nanoparticles also triggered the disassembly of insulin amyloid fibrils. Surface functionalization of amino acids appears to be important for the inhibition effect since isolated tryptophan and tyrosine molecules did not prevent insulin aggregation. Bioinformatics analysis predicts involvement of tyrosine in H-bonding interactions mediated by its C=O, -NH2, and aromatic moiety. These results offer significant opportunities for developing nanoparticle-based therapeutics against diseases related to protein aggregation.
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Old Residency Road, Jodhpur, Rajasthan, 342011, India.
authors.forename: Kriti
authors.initials: K
authors.lastname: Dubey
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Old Residency Road, Jodhpur, Rajasthan, 342011, India.
authors.forename: Bibin G
authors.initials: BG
authors.lastname: Anand
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Old Residency Road, Jodhpur, Rajasthan, 342011, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Badhwar
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Old Residency Road, Jodhpur, Rajasthan, 342011, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Bagler
authors.affiliation: Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, Karnataka, 572103, India.
authors.forename: P N
authors.initials: PN
authors.lastname: Navya
authors.affiliation: Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, Karnataka, 572103, India. hkdaima@gmail.com.
authors.forename: Hemant Kumar
authors.initials: HK
authors.lastname: Daima
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Old Residency Road, Jodhpur, Rajasthan, 342011, India. karunakarkar@gmail.com.
authors.forename: Karunakar
authors.initials: K
authors.lastname: Kar
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D019295:Computational Biology; D006046:Gold / Q000737:chemistry*; D006860:Hydrogen Bonding; D007328:Insulin / Q000737:chemistry*; D007329:Insulin Antagonists / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D062105:Molecular Docking Simulation; D011485:Protein Binding; D011487:Protein Conformation; D012834:Silver / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014364:Tryptophan / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000682:Amyloid; D007328:Insulin; D007329:Insulin Antagonists; D012834:Silver; D014443:Tyrosine; D006046:Gold; D014364:Tryptophan
keywords: Amyloid aggregation; Gold nanoparticles; Insulin; Tryptophan; Tyrosine