title: miR-1238 inhibits cell proliferation by targeting LHX2 in non-small cell lung cancer.
abstract: In human cancers, dysregulated expression of LIM-homeobox gene 2 (LHX2) and downregulation of miR-1238 has been reported separately. However, the relationship between them remains unclear. We investigated the functional contribution of miR-1238 to the regulation of LHX2 in non-small cell lung cancer (NSCLC). Here, computational algorithms predicted that the 3'-untranslated region (3'-UTR) of LHX2 is a target of miR-1238. Luciferase assays validated that miR-1238 directly bound to 3'-UTR of LHX2. qRT-PCR and western blot analyses further confirmed that overexpression of miR-1238 mimic in NSCLC A549 and LTEP-α-2 cells inhibited endogenous expression of LHX2 mRNA and protein. Moreover, ectopic expression of miR-1238 in NSCLC A549 and LTEP-α-2 cells suppressed cellular viability and proliferation. siRNA-induced knockdown of LHX2 copied the phenotype of miR-1238 overexpression in NSCLC A549 and LTEP-α-2 cells and LHX2 knockdown inhibited cell cycle. In addition, miR-1238 expression was frequently decreased in human NSCLC tissues and reversely correlated with LHX2 expression, which was increased in NSCLC tissues. Collectively, our findings demonstrate that miR-1238 inhibit the proliferation of NSCLC cells at least partly via repression of LHX2, shedding light on the mechanistic interaction of miR-1238 and LHX2 in NSCLC carcinogenesis. Furthermore, our data suggest that expression of miR-1238 could be a promising therapeutic strategy for NSCLC treatment.
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.|Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.
authors.forename: Xiangguang
authors.initials: X
authors.lastname: Shi
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.|Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhan
authors.affiliation: The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou, China.
authors.forename: Can
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.|Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Lei
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.|Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Yang
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.|Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.
authors.forename: Longqiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.|The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.|Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C489783:LHX2 protein, human; D060850:LIM-Homeodomain Proteins; C000608595:MIRN1238 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors
keywords: LHX2; NSCLC; cell proliferation; miR-1238