title: Batch production of coenzyme Q10 by recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Sphingomonas baekryungensis.
abstract: Coenzyme Q10 (CoQ10) is an important antioxidant used in medicine, dietary supplements, and cosmetic applications. In the present work, the production of CoQ10 using a recombinant Escherichia coli strain containing the decaprenyl diphosphate synthase from Sphingomonas baekryungensis was investigated, wherein the effects of culture medium, temperature, and agitation rate on the production process were assessed. It was found that Luria-Bertani (LB) medium was superior to M9 with glucose medium. Higher temperature (37 °C) and higher agitation rate (900 rpm) improved the specific CoQ10 content significantly in LB medium; on the contrary, the use of M9 medium with glucose showed similar values. Specifically, in LB medium, an increase from 300 to 900 rpm in the agitation rate resulted in increases of 55 and 197 % in the specific CoQ10 content and COQ10 productivity, respectively. Therefore, the results obtained in the present work are a valuable contribution for the optimization of CoQ10 production processes using recombinant E. coli strains.
authors.affiliation: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile, irene.martinez@ucv.cl.
authors.forename: Irene
authors.initials: I
authors.lastname: Martínez
authors.forename: Claudia
authors.initials: C
authors.lastname: Méndez
authors.forename: Julio
authors.initials: J
authors.lastname: Berríos
authors.forename: Claudia
authors.initials: C
authors.lastname: Altamirano
authors.forename: Alvaro
authors.initials: A
authors.lastname: Díaz-Barrera
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D019149:Bioreactors; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering; D020578:Sphingomonas / Q000201:enzymology* / Q000235:genetics; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D014451:Ubiquinone; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase; C024989:coenzyme Q10