title: Neuroprotective effect of interleukin-6 regulation of voltage-gated Na(+) channels of cortical neurons is time- and dose-dependent.
abstract: Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour exposure of interleukin-6 on cortical neurons at various concentrations (0.1, 1, 5 and 10 ng/mL) and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations (2, 4, 8, 24 and 48 hours) by studying voltage-gated Na(+) channels using a patch-clamp technique. Voltage-clamp recording results demonstrated that interleukin-6 suppressed Na(+) currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na(+) channels in rat cortical neurons by interleukin-6 is time- and dose-dependent.
authors.affiliation: Department of Interventional Radiology, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China.
authors.forename: Guo-Yi
authors.initials: GY
authors.lastname: Peng
authors.affiliation: Department of Pathogenic Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province, China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Sheng
authors.affiliation: Department of Pathogenic Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province, China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China.
authors.forename: Jing-Fang
authors.initials: JF
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Chen
keywords: NSFC grants; action potential; brain injury; cerebrospinal fluid; cortical neurons; inflammatory reaction; interleukin-6; nerve regeneration; neural regeneration; neuroimmunomodulation; neurophysiology; neuroprotection; patch clamp; voltage-gated Na+ channel