title: Anisakis pegreffii Larvae in Sea Eels (Astroconger myriaster) from the South Sea, Republic of Korea.
abstract: Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jaeeun
authors.initials: J
authors.lastname: Cho
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Hyemi
authors.initials: H
authors.lastname: Lim
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Bong-Kwang
authors.initials: BK
authors.lastname: Jung
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea ; Tropical Medicine, Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Shin
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
mesh_terms: D000818:Animals; D017129:Anisakiasis / Q000469:parasitology / Q000662:veterinary*; D017163:Anisakis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018509:DNA, Helminth / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004524:Eels / Q000254:growth & development; D005393:Fish Diseases / Q000469:parasitology*; D007814:Larva / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D056910:Republic of Korea
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer
keywords: Anisakis pegreffii; ITS1; PCR-RFLP; cox2; sea eel