title: Modulation of Melanogenesis and Antioxidant Status of Melanocytes in Response to Phototoxic Action of Doxycycline.
abstract: Doxycycline is a commonly used tetracycline antibiotic showing the broad spectrum of antibacterial action. However, the use of this antibiotic is often connected with the risk of phototoxic reactions that lead to various skin disorders. One of the factors influencing the photosensitivity reactions is the melanin content in melanocytes. In this study, the impact of doxycycline and UVA irradiation on cell viability, melanogenesis and antioxidant defense system in cultured normal human epidermal melanocytes (HEMn-DP) was examined. The exposure of cells to doxycycline and UVA radiation resulted in concentration-dependent loss in melanocytes viability and induced melanin biosynthesis. Significant changes were stated in cellular antioxidant enzymes activity: SOD, CAT and GPx, which indicates alterations of antioxidant defense system. The results obtained in vitro may explain the mechanisms of phototoxic reactions that occur in normal human epidermal melanocytes in vivo after exposure of skin to doxycycline and UVA radiation.
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Rok
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Buszman
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Beberok
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Delijewski
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Otręba
authors.affiliation: Chair and Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Wrześniok
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival; D017484:Dermatitis, Phototoxic; D004318:Doxycycline / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects; D006801:Humans; D008544:Melanocytes / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D004318:Doxycycline