title: Synthesis and characterization of zinc sulfide quantum dots and their interaction with snake gourd (Trichosanthes anguina) seed lectin.
abstract: Owing to the use of quantum dots in biological labeling, and the specific interaction of lectins with tumor cells, studies on lectin-QDs interaction are of potential interest. Herein, we report a facile method to prepare zinc sulfide quantum dots (ZnS QDs) using pectin as a capping agent and studied their interaction with snake gourd seed lectin (SGSL) by fluorescence spectroscopy. The QDs were characterized by X-ray diffraction, high-resolution transmission electron microscopy, UV-Vis absorption and fluorescence spectroscopy. The thermodynamic forces governing the interaction between ZnS-QDs and SGSL have been delineated from the temperature dependent association constant. These results suggest that the binding between ZnS QDs and SGSL is governed by enthalpic forces with negative entropic contribution. The red shift of synchronous fluorescence spectra showed that the microenvironment around the tryptophan residues of SGSL was perturbed by ZnS-QDs. The obtained results suggest that the development of optical bioimaging agents by using the conjugated lectin-QDs would be possible to diagnose cancerous tissues.
authors.affiliation: Department of Chemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Khan Behlol
authors.initials: KB
authors.lastname: Ayaz Ahmed
authors.affiliation: Department of Chemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Pichaikkannu
authors.initials: P
authors.lastname: Ahalya
authors.affiliation: Department of Chemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Megarajan
authors.initials: M
authors.lastname: Sengan
authors.affiliation: Environmental and Analytical Chemistry Division, School of Advance Sciences, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
authors.forename: Ravikanth
authors.initials: R
authors.lastname: Kamlekar
authors.affiliation: Department of Chemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India. Electronic address: anbazhagan@scbt.sastra.edu.
authors.forename: Anbazhagan
authors.initials: A
authors.lastname: Veerappan
mesh_terms: D060326:Chemistry Techniques, Synthetic; D019277:Entropy; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013440:Sulfides / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D029778:Trichosanthes / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D037102:Lectins; D013440:Sulfides; D017967:Zinc Compounds; D014364:Tryptophan; C031238:zinc sulfide
keywords: Binding; Lectin; Pectin; Snake gourd seed lectin; Thermodynamics; Zinc sulfide quantum dots