title: Reduction of aflatoxins (B₁, B₂, G₁, and G₂) in soybean-based model systems.
abstract: The effects of chemical, physical, and cooking treatments on the reduction of aflatoxin B1 (AFB1), B2, G1, and G2 in soybean matrix were investigated. A HPLC-FLD with a Kobra cell system was used for the quantitative analysis of aflatoxins (AFs). To decrease the level of AFs during the soaking process, the contaminated soybeans were submerged in organic acid solutions. The reduction rates of AFB1 in 1.0N citric acid, lactic acid, succinic acid, and tartaric acid for 18h were 94.1%, 92.7%, 62.0%, and 95.1%, respectively. In the case of pH and autoclave treatment, the level of AFB1 was significantly decreased during autoclaving process at pH 7.4, 9.0, and 11.1, compared with the non-autoclaved samples (p<0.05). In the case of physical treatment, the heating process at 100 and 150°C for 90min significantly decreased the level of AFB1 by 41.9% and 81.2%, respectively (p<0.05). The reduction rate of AFB1 after cooking was 97.9% for soybean milk and 33.6% for steamed soybeans.
authors.affiliation: Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Jung-gu, Seoul 100-715, Republic of Korea.
authors.forename: Jongin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Jung-gu, Seoul 100-715, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Her
authors.affiliation: Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Jung-gu, Seoul 100-715, Republic of Korea. Electronic address: kwglee@dongguk.edu.
authors.forename: Kwang-Geun
authors.initials: KG
authors.lastname: Lee
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000348:Aflatoxins / Q000032:analysis*; D055598:Chemical Phenomena; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000517:prevention & control*; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008954:Models, Biological; D015203:Reproducibility of Results; D041942:Soy Milk / Q000737:chemistry; D013025:Glycine max / Q000382:microbiology*; D013644:Tartrates / Q000737:chemistry
chemical_list: D000348:Aflatoxins; D013644:Tartrates; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1; C029768:tartaric acid
keywords: Aflatoxins (AFs); Model systems; Reduction; Soybean