title: Analysis of nanoparticle-protein coronas formed in vitro between nanosized welding particles and nasal lavage proteins.
abstract: Welding fumes include agglomerated particles built up of primary nanoparticles. Particles inhaled through the nose will to some extent be deposited in the protein-rich nasal mucosa, and a protein corona will be formed around the particles. The aim was to identify the protein corona formed between nasal lavage proteins and four types of particles with different parameters. Two of the particles were formed and collected during welding and two were manufactured iron oxides. When nasal lavage proteins were added to the particles, differences were observed in the sizes of the aggregates that were formed. Measurements showed that the amount of protein bound to particles correlated with the relative size increase of the aggregates, suggesting that the surface area was associated with the binding capacity. However, differences in aggregate sizes were detected when nasal proteins were added to UFWF and Fe2O3 particles (having similar agglomerated size) suggesting that yet parameters other than size determine the binding. Relative quantitative mass spectrometric and gel-based analyses showed differences in the protein content of the coronas. High-affinity proteins were further assessed for network interactions. Additional experiments showed that the inhibitory function of secretory leukocyte peptidase inhibitor, a highly abundant nasal protein, was influenced by particle binding suggesting that an understanding of protein function following particle binding is necessary to properly evaluate pathophysiological events. Our results underscore the importance of including particles collected from real working environments when studying the toxic effects of particles because these effects might be mediated by the protein corona.
authors.affiliation: a Division of Occupational and Environmental Medicine , Lund University , Lund , Sweden .
authors.forename: Neserin
authors.initials: N
authors.lastname: Ali
authors.affiliation: b Center for Molecular Protein Science, Biochemistry and Structural Biology, Lund University , Lund , Sweden .
authors.forename: Karin
authors.initials: K
authors.lastname: Mattsson
authors.affiliation: c Department of Design Sciences , Ergonomic and Aerosol Technology, Lund University , Lund , Sweden , and.
authors.forename: Jenny
authors.initials: J
authors.lastname: Rissler
authors.affiliation: d Occupational and Environmental Medicine, County Council of Östergötland, Linköping University , Linköping , Sweden.
authors.forename: Helen Marg
authors.initials: HM
authors.lastname: Karlsson
authors.affiliation: c Department of Design Sciences , Ergonomic and Aerosol Technology, Lund University , Lund , Sweden , and.
authors.forename: Christian R
authors.initials: CR
authors.lastname: Svensson
authors.affiliation: c Department of Design Sciences , Ergonomic and Aerosol Technology, Lund University , Lund , Sweden , and.
authors.forename: Anders
authors.initials: A
authors.lastname: Gudmundsson
authors.affiliation: a Division of Occupational and Environmental Medicine , Lund University , Lund , Sweden .
authors.forename: Christian H
authors.initials: CH
authors.lastname: Lindh
authors.affiliation: a Division of Occupational and Environmental Medicine , Lund University , Lund , Sweden .
authors.forename: Bo A G
authors.initials: BA
authors.lastname: Jönsson
authors.affiliation: b Center for Molecular Protein Science, Biochemistry and Structural Biology, Lund University , Lund , Sweden .
authors.forename: Tommy
authors.initials: T
authors.lastname: Cedervall
authors.affiliation: a Division of Occupational and Environmental Medicine , Lund University , Lund , Sweden .
authors.forename: Monica
authors.initials: M
authors.lastname: Kåredal
mesh_terms: D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013058:Mass Spectrometry; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D010316:Particle Size; D000066970:Protein Corona / Q000032:analysis / Q000737:chemistry*; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000378:metabolism; D014896:Welding
chemical_list: D058185:Magnetite Nanoparticles; D000066970:Protein Corona; D053481:Secretory Leukocyte Peptidase Inhibitor
keywords: Mass spectrometry; nanoparticles; nanotoxicology; protein corona; proteomics