title: Uric acid variation among regular blood donors is indicative of red blood cell susceptibility to storage lesion markers: A new hypothesis tested.
abstract: BACKGROUND
  Oxidative stress orchestrates a significant part of the red blood cell (RBC) storage lesion. Considering the tremendous interdonor variability observed in the "storability," namely, the capacity of RBCs to sustain the storage lesion, this study aimed at the elucidation of donor-specific factors that affect the redox homeostasis during the storage of RBCs in standard systems.
  
  
  METHODS
  The hematologic profile of regular blood donors (n = 78) was evaluated by biochemical analysis of 48 different variables, including in vivo hemolysis and plasma oxidant and antioxidant factors and statistical analysis of the results. The possible effect of the uric acid (UA) variable on RBC storability was investigated in leukoreduced CPD/SAGM RBC units (n = 8) collected from donors exhibiting high or low prestorage levels of UA, throughout the storage period.
  
  
  RESULTS
  Among the hematologic variables examined in vivo, cluster analysis grouped the donors according to their serum UA levels. Plasma antioxidant capacity, iron indexes, and protein carbonylation represented covariants of UA factor. RBCs prepared by low- or high-UA donors exhibited significant differences between them in spheroechinocytosis, supernatant antioxidant activity, and other RBC storage lesion-associated variables.
  
  
  CONCLUSIONS
  UA exhibits a storability biomarker potential. Intrinsic variability in plasma UA levels might be related to the interdonor variability observed in the storage capacity of RBCs. A model for the antioxidant effect of UA during the RBC storage is currently proposed.
authors.affiliation: Department of Cell Biology and Biophysics, Faculty of Biology, NKUA.
authors.forename: Vassilis L
authors.initials: VL
authors.lastname: Tzounakas
authors.affiliation: Department of Cell Biology and Biophysics, Faculty of Biology, NKUA.
authors.forename: Hara T
authors.initials: HT
authors.lastname: Georgatzakou
authors.affiliation: Laboratory of Hematology and Transfusion Medicine, Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens, Athens.
authors.forename: Anastasios G
authors.initials: AG
authors.lastname: Kriebardis
authors.affiliation: Laboratory of Information Science and Biostatistics, Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens; and.
authors.forename: Effie G
authors.initials: EG
authors.lastname: Papageorgiou
authors.affiliation: Hellenic National Blood Center, Acharnes, Athens, Greece.|Regional Blood Transfusion Center, "Agios Panteleimon" General Hospital of Nikea, Piraeus, Greece.
authors.forename: Konstantinos E
authors.initials: KE
authors.lastname: Stamoulis
authors.affiliation: Regional Blood Transfusion Center, "Agios Panteleimon" General Hospital of Nikea, Piraeus, Greece.
authors.forename: Leontini E
authors.initials: LE
authors.lastname: Foudoulaki-Paparizos
authors.affiliation: Department of Cell Biology and Biophysics, Faculty of Biology, NKUA.
authors.forename: Marianna H
authors.initials: MH
authors.lastname: Antonelou
authors.affiliation: Department of Cell Biology and Biophysics, Faculty of Biology, NKUA.
authors.forename: Issidora S
authors.initials: IS
authors.lastname: Papassideri
mesh_terms: D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D004198:Disease Susceptibility; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D014527:Uric Acid / Q000097:blood*; D055815:Young Adult
chemical_list: C051020:SAG-M blood; D014527:Uric Acid