title: Significance of gastrin-releasing peptide in ovarian cancer ES2 cells.
abstract: The present study aimed to investigate the effect of gastrin-releasing peptide (GRP) on the proliferation and invasion of ovarian cancer ES2 cells. The ovarian cancer ES2 cells were transfected with small interfering RNA against GRP. Cell proliferation was assessed using the Trypan blue assay, apoptosis was determined using propidium iodide/fluorescein isothiocyanate and flow cytometry, and the invasion ability was detected using the Transwell assay. The results revealed that the expression of GRP significantly decreased following transfection with GRP-short hairpin RNA. Furthermore, the silencing of GRP resulted in increased apoptosis and a reduced invasive ability of the ES2 cells. It was concluded that GRP may regulate the proliferation and migration of human ovarian cancer cells, which indicates that GRP may be a potential novel target for the treatment of ovarian cancer.
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, Henan Scientific and Technologic University, Luoyang, Henan 471003, P.R. China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Fan
keywords: cell proliferation; gastrin-releasing peptide; ovarian cancer; small interfering RNA