title: lobChIP: from cells to sequencing ready ChIP libraries in a single day.
abstract: BACKGROUND
  ChIP-seq is the method of choice for genome-wide studies of protein-DNA interactions. We describe a new method for ChIP-seq sample preparation, termed lobChIP, where the library reactions are performed on cross-linked ChIP fragments captured on beads.
  
  
  RESULTS
  The lobChIP method was found both to reduce time and cost and to simplify the processing of many samples in parallel. lobChIP has an early incorporation of barcoded sequencing adaptors that minimizes the risk of sample cross-contamination and can lead to reduced amount of adaptor dimers in the sequencing libraries, while allowing for direct decross-linking and amplification of the sample.
  
  
  CONCLUSIONS
  With results for histone modifications and transcription factors, we show that lobChIP performs equal to or better than standard protocols and that it makes it possible to go from cells to sequencing ready libraries within a single day.
authors.affiliation: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Uppsala University, Box 815, 75108 Uppsala, Sweden ; Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Ola
authors.initials: O
authors.lastname: Wallerman
authors.affiliation: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Uppsala University, Box 815, 75108 Uppsala, Sweden.
authors.forename: Helena
authors.initials: H
authors.lastname: Nord
authors.affiliation: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Uppsala University, Box 815, 75108 Uppsala, Sweden ; Department of Clinical Sciences, CRC, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Madhusudhan
authors.initials: M
authors.lastname: Bysani
authors.affiliation: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Uppsala University, Box 815, 75108 Uppsala, Sweden ; Human Genetics, Genome Institute of Singapore, Singapore, Singapore.
authors.forename: Lisa
authors.initials: L
authors.lastname: Borghini
authors.affiliation: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Uppsala University, Box 815, 75108 Uppsala, Sweden.
authors.forename: Claes
authors.initials: C
authors.lastname: Wadelius
keywords: ChIP-seq; Chromatin immunoprecipitation; Illumina; Library preparation; NGS