title: Pro-Angiogenic Effects of Chalcone Derivatives in Zebrafish Embryos in Vivo.
abstract: The aim of this study was to investigate novel chalcones with potent angiogenic activities in vivo. Chalcone-based derivatives were evaluated using a transgenic zebrafish line with fluorescent vessels to real-time monitor the effect on angiogenesis. Results showed that the chalcone analogues did not possess anti-angiogenic effect on zebrafish vasculatures; instead, some of them displayed potent pro-angiogenic effects on the formation of the sub-intestinal vein. Similar pro-angiogenic effects can also be seen on wild type zebrafish embryos. Moreover, the expression of vegfa, the major regulator for angiogenesis, was also upregulated in their treatment. Taken together, we have synthesized and identified a series of novel chalcone-based derivatives as potent in vivo pro-angiogenic compounds. These novel compounds hold potential for therapeutic angiogenesis.
authors.affiliation: Department of Chemistry, Tamkang University, 151, Ying-chuan Road, Danshui District, New Taipei City 25137, Taiwan. yauhung@mail.tku.edu.tw.
authors.forename: Yau-Hung
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Tamkang University, 151, Ying-chuan Road, Danshui District, New Taipei City 25137, Taiwan. frank00634@hotmail.com.
authors.forename: Chao-Yuan
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan. kitomint@hotmail.com.
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Chang
authors.affiliation: Department of Applied Chemistry, National Chia-Yi University, Chia-Yi 60004, Taiwan. tpdyha6606@gmail.com.
authors.forename: Po-Chih
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Tamkang University, 151, Ying-chuan Road, Danshui District, New Taipei City 25137, Taiwan. joanna20520@gmail.com.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, National Chia-Yi University, Chia-Yi 60004, Taiwan. cychern@mail.ncyu.edu.tw.
authors.forename: Ching-Yuh
authors.initials: CY
authors.lastname: Chern
authors.affiliation: School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan. jeningts@csmu.edu.tw.|Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 40201, Taiwan. jeningts@csmu.edu.tw.
authors.forename: Jen-Ning
authors.initials: JN
authors.lastname: Tsai
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000818:Animals; D030801:Animals, Genetically Modified; D047188:Chalcones / Q000138:chemical synthesis / Q000494:pharmacology*; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D015394:Molecular Structure; D009024:Morphogenesis / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D013329:Structure-Activity Relationship; D042461:Vascular Endothelial Growth Factor A / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000819:agonists* / Q000235:genetics / Q000378:metabolism
chemical_list: D043925:Angiogenesis Inducing Agents; D047188:Chalcones; D042461:Vascular Endothelial Growth Factor A; D029961:Zebrafish Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: angiogenesis; chalcones; in vivo; zebrafish embryos