title: The use of dried blood spot specimens for HIV-1 drug resistance genotyping in young children initiating antiretroviral therapy.
abstract: Paired plasma and dried blood spots (DBS) from 232 South African HIV-infected children initiating antiretroviral therapy (ART) were genotyped for drug resistance mutations, most of who had prior exposure to ART for prevention-of-mother-to-child-transmission. Non-nucleoside reverse transcriptase inhibitor mutations were most commonly detected in both specimen types, particularly Y181C/I and K103N/S. Resistance interpretation concordance was achieved in 97% of pairs with seven children having mutations detected in DBS only. These results validate the preferential use of DBS specimens for HIVDR genotyping in this patient group.
authors.affiliation: Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa. Electronic address: Anna.Salimo@nhls.ac.za.
authors.forename: Anna T
authors.initials: AT
authors.lastname: Salimo
authors.affiliation: Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa. Electronic address: Johannal@nicd.ac.za.
authors.forename: Johanna
authors.initials: J
authors.lastname: Ledwaba
authors.affiliation: Empilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: ashraf.coovadia@wits.ac.za.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Coovadia
authors.affiliation: ICAP, Mailman School of Public Health, and College of Physicians & Surgeons, Columbia University, New York, USA. Electronic address: eja1@cumb.columbia.edu.
authors.forename: Elaine J
authors.initials: EJ
authors.lastname: Abrams
authors.affiliation: Empilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: karl-gunter.technau@wits.ac.za.
authors.forename: Karl-Günter
authors.initials: KG
authors.lastname: Technau
authors.affiliation: Gertrude H. Sergievsky Center, College of Physicians and Surgeons, and Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, USA. Electronic address: lk24@cumc.columbia.edu.
authors.forename: Louise
authors.initials: L
authors.lastname: Kuhn
authors.affiliation: Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: lynnm@nicd.ac.za.
authors.forename: Lynn
authors.initials: L
authors.lastname: Morris
authors.affiliation: Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: gillianh@nicd.ac.za.
authors.forename: Gillian M
authors.initials: GM
authors.lastname: Hunt
mesh_terms: D001769:Blood / Q000821:virology*; D002675:Child, Preschool; D003890:Desiccation; D024882:Drug Resistance, Viral; D060005:Genotyping Techniques / Q000379:methods*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D013019:South Africa; D013048:Specimen Handling / Q000379:methods*
keywords: Children; Dried blood spots; HIV drug resistance genotyping