title: Potential regulatory phosphorylation sites in a Medicago truncatula plasma membrane proton pump implicated during early symbiotic signaling in roots.
abstract: In plants and fungi the plasma membrane proton pump generates a large proton-motive force that performs essential functions in many processes, including solute transport and the control of cell elongation. Previous studies in yeast and higher plants have indicated that phosphorylation of an auto-inhibitory domain is involved in regulating pump activity. In this report we examine the Medicago truncatula plasma membrane proton pump gene family, and in particular MtAHA5. Yeast complementation assays with phosphomimetic mutations at six candidate sites support a phosphoregulatory role for two residues, suggesting a molecular model to explain early Nod factor-induced changes in the plasma membrane proton-motive force of legume root cells.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Thao T
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Volkening
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Rose
authors.affiliation: Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, United States; School of Agriculture, University of Wisconsin-Platteville, Platteville, WI 53818, United States.
authors.forename: Muthusubramanian
authors.initials: M
authors.lastname: Venkateshwaran
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Westphall
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Coon
authors.affiliation: Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Ané
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: msussman@wisc.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sussman
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology*; D016000:Cluster Analysis; D015971:Gene Expression Regulation, Enzymologic; D005816:Genetic Complementation Test; D054884:Host-Pathogen Interactions; D046913:Medicago truncatula / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010766:Phosphorylation; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D006180:Proton-Translocating ATPases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012231:Rhizobium / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013559:Symbiosis; D013912:Threonine / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013912:Threonine; D012694:Serine; D006180:Proton-Translocating ATPases
keywords: Medicago truncatula; Nod factor; PM H(+)-ATPase; Phosphoproteomics; Phosphorylation; Proton pump