title: Antioxidative and cytoprotective response elicited by molybdenum nanoparticles in human cells.
abstract: Nanotechnology based therapeutics can offer an alternative platform in a wide variety of biomedical applications. Here we report novel cytotoxicity preventive potential of molybdenum nanoparticles (Mo NPs) in human breast (MCF-7) and fibrosarcoma (HT-1080) cells compromised with oxidant exposure. Physicochemical properties such as size, crystallinity, purity and band gap (an optical characteristic) of Mo NPs were characterized respectively by field emission transmission electron microscopy (FETEM), X-ray diffraction (XRD), energy dispersive spectrum (EDS) and UV-vis absorption spectroscopy. The average size of crystalline Mo NPs was found to be 35 nm with a band gap of 1.4 eV. Potential cytotoxicity of Mo NPs was evaluated by a battery of cell viability and oxidative stress parameters. Cell viability and oxidative stress data suggested Mo NPs to be reasonably non-cytotoxic. Cytotoxic preventive and GSH restoring potential of Mo NPs was determined against cytotoxicity and oxidative stress induced by H2O2 (and ZnO NPs) in two cells. Mo NPs significantly increased GSH level in MCF-7 and HT-1080 cells, an activity that was comparable to antioxidant N-acetyl cysteine (NAC). GSH level was increased 1.56 times in MCF-7 cells and 1.25 times in HT-1080 cells by 100 μg/ml of Mo NPs relative to control cells in 24 h. End-point data clearly suggest that Mo NPs significantly protected cells against cytotoxicity induced by H2O2 and ZnO (NPs) (p<0.05). Our study warrants further investigation about Mo NPs that could be exploited in myriads of nanotechnology applications.
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia. Electronic address: mohd.j.akhtar@gmail.com.
authors.forename: Mohd Javed
authors.initials: MJ
authors.lastname: Akhtar
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Maqusood
authors.initials: M
authors.lastname: Ahamed
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia; Department of Medical Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Hisham A
authors.initials: HA
authors.lastname: Alhadlaq
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia; Nanomedicine Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Aws
authors.initials: A
authors.lastname: Alshamsan
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia.
authors.forename: M A Majeed
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Salman A
authors.initials: SA
authors.lastname: Alrokayan
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D061986:MCF-7 Cells; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D000975:Antioxidants; D016877:Oxidants; D008982:Molybdenum; D006861:Hydrogen Peroxide
keywords: Antioxidants NPs; Glutathione; Molybdenum NPs; Nanomedicine; Oxidants