title: Downregulation of microRNA-100 protects H2O2-induced apoptosis in neonatal cardiomyocytes.
abstract: Hypoxia or reoxygenation-induced cardiomyocyte apoptosis is one of the major causes of cardiac dysfunction. Recently, regulations of microRNAs were shown to play important roles in cardiomyocyte apoptosis. MicroRNA-100 (miR-100) is one of the cardiac miRNA that was up-regulated in failing heart. In this study, we identified that miR-100 expression was up-regulated in H2O2-induced apoptosis in neonatal mice cardiomyocytes in a time-dependent manner. Furthermore, functional analysis revealed that miR-100 downregulation attenuated H2O2-induced apoptosis. Through biochemical analysis of western blot, we found that miR-100 suppressed the expression of insulin-like growth factor 1 receptor (IGF1R) during the process of hypoxia-induced apoptosis in cardiomyocytes. More importantly, ectopic down-regulation of IGF1R reversed the protective effect of miR-100 down-regulation on H2O2-induced apoptosis, revealing that miR-100 regulates cardiomyocyte apoptosis through the association of IGF1R. Taken together, our data demonstrated the functional role miR-100 in H2O2-induced apoptosis in cardiac dysfunctions.
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, Guangdong Province, China.
authors.forename: An
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, Guangdong Province, China.
authors.forename: Gonghui
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, Guangdong Province, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, Guangdong Province, China.
authors.forename: Jingbin
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Cardiology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, Guangdong Province, China.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D015536:Down-Regulation; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C558180:Mirn100 microRNA, mouse; D006861:Hydrogen Peroxide; D017526:Receptor, IGF Type 1
keywords: Cardiomyocytes; IGIFR; apoptosis; microRNA-100