title: Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities.
abstract: Plant mediated synthesis of nanoparticles has been considered as green route and a reliable technique for the synthesis of nanoparticles due to its eco-friendly approach. In this study, we report a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using methanolic Momordica cymbalaria fruit extract as reducing agent. The fruit extract of M. cymbalaria exposed to AgNO3 solution showed the change in color from green to light yellow at room temperature within 1h of incubation confirms the synthesis of AgNPs. UV-vis spectra analysis revealed that the synthesized AgNPs had a sharp surface plasmon resonance at around 450 nm, while, the X-ray Diffraction (XRD) patterns confirmed distinctive peaks indices to the crystalline planes of the face centered cubic silver. The Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) analysis results confirmed the presence of spherical shaped AgNPs by a huge disparity in the particle size distribution with an average size of 15.5 nm. The synthesized AgNPs showed strong antibacterial activity against all the tested multidrug resistant human pathogenic bacterial strains and also exhibited highest free radical scavenging activity (74.2%) compared to fruit extract (60.4%). Moreover, both fruit extract and the synthesized AgNPs showed the cytotoxicity towards Rat L6 skeletal muscle cell line at different concentrations, but the highest inhibition percentage was recorded for AgNPs at concentration of 100 μg/ml.
authors.affiliation: Department of Biotechnology, Padmashree Institute of Management and Sciences, Kommagatta, Bangalore 560060, India; Department of Crop Science, Faculty of Agriculture, Universiti Purta Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: kumara@upm.edu.my.
authors.forename: Mallappa Kumara
authors.initials: MK
authors.lastname: Swamy
authors.affiliation: Institute of Tropical Agriculture, Universiti Purta Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: sayeedbot@gmail.com.
authors.forename: Mohd Sayeed
authors.initials: MS
authors.lastname: Akhtar
authors.affiliation: Department of Biotechnology, Padmashree Institute of Management and Sciences, Kommagatta, Bangalore 560060, India.
authors.forename: Sudipta Kumar
authors.initials: SK
authors.lastname: Mohanty
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Purta Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: umarani@upm.edu.my.
authors.forename: Uma Rani
authors.initials: UR
authors.lastname: Sinniah
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D029775:Momordica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts; D012834:Silver
keywords: Fruit extract; Nanotechnology; Pathogenic bacteria; Phytosynthesis; SEM; XRD