title: Comparative analysis of zygotic developmental genes in Rhodnius prolixus genome shows conserved features on the tracheal developmental pathway.
abstract: Most of the in-depth studies on insect developmental genetic have been carried out in the fruit fly Drosophila melanogaster, an holometabolous insect, so much more still remains to be studied in hemimetabolous insects. Having Rhodnius prolixus sequenced genome available, we search for orthologue genes of zygotic signaling pathways, segmentation, and tracheogenesis in the R. prolixus genome and in three species of Triatoma genus transcriptomes, concluding that there is a high level of gene conservation. We also study the function of two genes required for tracheal system development in D. melanogaster - R. prolixus orthologues: trachealess (Rp-trh) and empty spiracles (Rp-ems). From that we see that Rp-trh is required for early tracheal development since Rp-trh RNAi shows that the primary tracheal branches fail to form. On the other hand, Rp-ems is implied in the proper formation of the posterior tracheal branches, in a similar way to D. melanogaster. These results represent the initial characterization of the genes involved in the tracheal development of an hemimetabolous insect building a bridge between the current genomic era and V. Wigglesworth's classical studies on insects' respiratory system physiology.
authors.affiliation: Centro de Bioinvestigaciones and Departamento de Ciencias Básicas y Experimentales, Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Monteagudo 2772, 2700, Pergamino, Buenos Aires, Argentina. Electronic address: andreslavore@unnoba.edu.ar.
authors.forename: A
authors.initials: A
authors.lastname: Lavore
authors.affiliation: Centro de Bioinvestigaciones and Departamento de Ciencias Básicas y Experimentales, Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Monteagudo 2772, 2700, Pergamino, Buenos Aires, Argentina; Laboratorio de Genética y Genómica Funcional, Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Bvd 120 y 62, La Plata, Buenos Aires, Argentina. Electronic address: agustinapascual07@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Pascual
authors.affiliation: Centro de Bioinvestigaciones and Departamento de Ciencias Básicas y Experimentales, Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Monteagudo 2772, 2700, Pergamino, Buenos Aires, Argentina. Electronic address: salinasmaxif@gmail.com.
authors.forename: F M
authors.initials: FM
authors.lastname: Salinas
authors.affiliation: Laboratorio de Genética y Genómica Funcional, Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Bvd 120 y 62, La Plata, Buenos Aires, Argentina. Electronic address: nataieb@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Esponda-Behrens
authors.affiliation: Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, Sta. María Ahuacatitlán, Cuernavaca, Mexico. Electronic address: jmbarnet@insp.mx.
authors.forename: J
authors.initials: J
authors.lastname: Martinez-Barnetche
authors.affiliation: Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, Sta. María Ahuacatitlán, Cuernavaca, Mexico. Electronic address: mhenry@insp.mx.
authors.forename: M
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: Centro de Bioinvestigaciones and Departamento de Ciencias Básicas y Experimentales, Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Monteagudo 2772, 2700, Pergamino, Buenos Aires, Argentina; Laboratorio de Genética y Genómica Funcional, Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Bvd 120 y 62, La Plata, Buenos Aires, Argentina. Electronic address: rrivera@unnoba.edu.ar.
authors.forename: R
authors.initials: R
authors.lastname: Rivera-Pomar
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012239:Rhodnius / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014132:Trachea / Q000196:embryology / Q000378:metabolism; D059467:Transcriptome; D014224:Triatoma / Q000196:embryology / Q000235:genetics
chemical_list: D019476:Insect Proteins
keywords: Developmetal genes; Empty spiracles; Rhodnius prolixus; Trachealess; Tracheogenesis