title: A new "off-on" fluorescent probe for Al(3+) in aqueous solution based on rhodamine B and its application to bioimaging.
abstract: In this paper, a new fluorescent probe has been synthesized and applied as "off-on" sensor for the detection of Al(3+) with a high sensitivity and excellent selectivity in aqueous media. The sensor was easily prepared by one step reaction between rhodamine B hydrazide and pyridoxal hydrochloride named RBP. The structure of the sensor has been characterized by nuclear magnetic resonance and electron spray ionization-mass spectrometry. The fluorescence intensity and absorbance for the sensor showed a good linearity with the concentration of Al(3+) in the range of 0-12.5μM and 8-44μM, respectively, with detection limits of 0.23μM and 1.90μM. The sensor RBP was preliminarily applied to the determination of Al(3+) in water samples from the lake of Henan University and tap water with satisfying results. Moreover, it can be used as a bioimaging reagent for imaging of Al(3+) in living cells.
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China. Electronic address: zhqingyou@henu.edu.cn.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Enze
authors.initials: E
authors.lastname: Wang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China. Electronic address: zhouyanmei@henu.edu.cn.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Lanfang
authors.initials: L
authors.lastname: Pang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
mesh_terms: D000535:Aluminum / Q000032:analysis*; D000818:Animals; D002412:Cations / Q000032:analysis; D002460:Cell Line; D060766:Drinking Water / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry; D060106:Lakes / Q000032:analysis*; D018613:Microscopy, Confocal; D061848:Optical Imaging; D051381:Rats; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D002412:Cations; D060766:Drinking Water; D005456:Fluorescent Dyes; D006834:Hydrazines; D012235:Rhodamines; C543681:rhodamine B hydrazide; D000535:Aluminum; C029773:rhodamine B
keywords: Al(3+); Aqueous solution; Cellular imaging; Fluorescent sensor; Rhodamine B