title: Endoplasmic reticulum ribosome-binding protein 1, RRBP1, promotes progression of colorectal cancer and predicts an unfavourable prognosis.
abstract: BACKGROUND
  Ribosome-binding protein 1 (RRBP1) has been implicated in the regulation of unfolded protein response, which is involved in almost every aspect of cancer development. We aimed to explore the significance of RRBP1 in the progression and prognosis of colorectal cancer (CRC).
  
  
  METHODS
  The study population consisted of 856 patients with stage I-III CRC from two hospitals. RRBP1 expression was examined by immunohistochemistry (IHC) in colorectal tissues. The correlation of RRBP1 expression and CRC occurrence was assessed in paired cancer-adjacent tissues. Factors contributing to prognosis were evaluated in a training-validation design with univariate and multivariate Cox analysis. Colorectal cancer aggressiveness caused by RRBP1 knockdown or overexpression was evaluated in CRC cells.
  
  
  RESULTS
  RRBP1 was aberrantly overexpressed in CRC. Compared with low-RRBP1 patients, high-RRBP1 patients had shorter disease-specific survival in the training (hazard ratio (HR), 2.423; 95% confidence interval (CI), 1.531-3.835) and validation cohorts (HR, 3.749; 95% CI, 2.166-6.448) in multivariate Cox analysis. High-RRBP1 independently predicted a shorter disease-free survival (HR, 4.821; 95% CI, 3.220-7.218) in the validation cohort. RRBP1 knockdown reduced the aggressiveness of CRC cells in vitro and inhibited the growth of CRC xenografts in vivo.
  
  
  CONCLUSIONS
  High RRBP1 expression facilitates CRC progression and predicts an unfavourable post-operative prognosis.
authors.affiliation: Department of Digestive Endoscopy, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Shanghai, 201203, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 174 Changhai Road, Shanghai, 200433, China.
authors.forename: F
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Internal Medicine, Yangpu Hosptial, Tongji University School of Medicine, 450 Tengyue Road, Shanghai, 200090, China.
authors.forename: A
authors.initials: A
authors.lastname: Guo
authors.affiliation: Department of Environmental Hygiene, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, China.
authors.forename: W
authors.initials: W
authors.lastname: Chang
authors.affiliation: Department of Environmental Hygiene, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Environmental Hygiene, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, China.
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 174 Changhai Road, Shanghai, 200433, China.
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Pathology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Shanghai, 201203, China.
authors.forename: S
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 174 Changhai Road, Shanghai, 200433, China.
authors.forename: C
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Environmental Hygiene, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000368:Aged; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D002352:Carrier Proteins; C000602809:RRBP1 protein, human