title: Immunopotentiator from Pantoea agglomerans Prevents Atopic Dermatitis Induced by Dermatophagoides farinae Extract in NC/Nga Mouse.
abstract: OBJECTIVE
  Pantoea agglomerans LPS (immunopotentiator from Pantoea agglomerans 1: IP-PA1) has been reported to have anti-inflammatory effects in in vitro and in vivo models. The aim of the present study was to investigate the effects of orally-administered IP-PA1 on atopic dermatitis (AD) symptoms induced by Dermatophagoides farinae body extract (DFE) in NC/Nga mice.
  
  
  METHODS
  Using the NC/Nga AD murine model, mice were orally administered 0.1% (High) or 0.01% (Low) water-containing IP-PA1. Skin lesion assessment and blood collection from the caudal vein was performed on days 0, 7, 21 and 31. On day 31, all mice were sacrificed and blood, skin, spleen, as well as intestine samples, were obtained.
  
  
  RESULTS
  Assessment score of the skin lesion and serum immunoglobulin E (IgE) level of both IP-PA1 groups were significantly lower than that of the DFE group on days 14 and 21. The serum periostin and thymus and activation-regulated chemokine (TARC) level of IP-PA1-Low group was significantly lower than that of the DFE group on day 31. On histological examination of the skin, hyperplasia of epidermal and dermal layers and infiltration of inflammatory cells were suppressed by IP-PA1 administration. Deposition of periostin was observed in the DFE group skin tissue. Moreover, the CD4(+)/CD8(+) ratio of splenic T-cells increased by IP-PA1 administration.
  
  
  CONCLUSIONS
  IP-PA1 administration may have an inhibitory effect on AD skin lesions.
authors.affiliation: Department of Pharmacology, Hokkaido Pharmaceutical University School of Pharamacy, Sapporo, Hokkaido, Japan wakame-k@hokuyakudai.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Wakame
authors.affiliation: Department of Pharmacology, Hokkaido Pharmaceutical University School of Pharamacy, Sapporo, Hokkaido, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Komatsu
authors.affiliation: Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Kagawa, Japan Control of Innate Immunity, Technology Research Association, Takamatsu-shi, Kagawa, Japan Macrophi Inc., Takamatsu-shi, Kagawa, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inagawa
authors.affiliation: Macrophi Inc., Takamatsu-shi, Kagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishizawa
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D016516:CD4-CD8 Ratio; D015815:Cell Adhesion Molecules / Q000097:blood; D054414:Chemokine CCL17 / Q000097:blood; D003876:Dermatitis, Atopic / Q000276:immunology / Q000517:prevention & control*; D040001:Dermatophagoides farinae / Q000276:immunology; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020636:Pantoea / Q000276:immunology* / Q000378:metabolism; D012867:Skin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; C499538:Ccl17 protein, mouse; D015815:Cell Adhesion Molecules; D054414:Chemokine CCL17; D008070:Lipopolysaccharides; C082838:Postn protein, mouse; D007073:Immunoglobulin E
keywords: NC/Nga mice; Pantoea agglomerans; atopic dermatitis; dermatophagoides farinae body extract; lipopolysaccharide