title: Two new automated, compared with two enzyme-linked immunosorbent, antimüllerian hormone assays.
abstract: OBJECTIVE
  To compare new automated antimüllerian hormone (AMH) assay performance characteristics from the new automated Elecsys AMH (Roche; Elecsys) and Access AMH (Beckman Coulter; Access) assays with the existing AMH Gen II ELISA (enzyme-linked immunosorbent assay; Gen II; Beckman Coulter) and AMH ELISA (Ansh Labs) assays.
  
  
  METHODS
  Prospective assay evaluation.
  
  
  METHODS
  University-affiliated clinical chemistry laboratory.
  
  
  METHODS
  Patients referred for serum AMH measurement (n = 83) before start of in vitro fertilization cycle between September 2014 and October 2014.
  
  
  METHODS
  None.
  
  
  METHODS
  Serum AMH concentration.
  
  
  RESULTS
  Intra-assay coefficients of variation were low; Ansh ≤ 9.0%; Gen II ≤ 5.8%; Access ≤ 10.7%; and Elecsys ≤ 2.8%. The Passing-Bablok regression equations (pmol/L) were y (Access) = 0.128 + (0.781 × Gen II); and y (Access) = 0.302 + (0.742 x Ansh). For y (Elecys) = 0.087 + (0.729 x Gen II) and y (Elecys) = 0.253 + (0.688 x Ansh Labs). For y (Elecys) = 0.943 - (0.037 × Access). For all the assays, AMH exhibited a moderate positive correlation with AFC (r = 0.62-0.64); number of cumulus oocyte complexes (r = 0.60-0.64); and metaphase II oocytes (r = 0.48-0.50). Accuracy of pregnancy prediction, as determined by area under the receiver operating characteristic curve, was uniformly low for all assays (0.62-0.63).
  
  
  CONCLUSIONS
  The novel automated assays exhibit strong concordance in calibration, but derived values are substantially lower than those obtained from pre-existing assays, with assay-specific interpretation required for routine clinical use. These results highlight the need for an international standard of measurement of AMH.
authors.affiliation: School of Medicine, University of Glasgow, Glasgow, United Kingdom. Electronic address: scott.nelson@glasgow.ac.uk.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Nelson
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland; Department of Nursing, Medical University, Gdansk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pastuszek
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kloss
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Malinowska
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Liss
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland.
authors.forename: Aron
authors.initials: A
authors.lastname: Lukaszuk
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Plociennik
authors.affiliation: NVICTA Fertility and Reproductive Center, Gdansk, Poland; Department of Nursing, Medical University, Gdansk, Poland; INVICTA Fertility and Reproductive Center, Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lukaszuk
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000032:analysis* / Q000097:blood; D057205:Automation, Laboratory / Q000379:methods; D064790:Clinical Laboratory Services; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007164:Immunosorbents; D007246:Infertility / Q000097:blood / Q000628:therapy; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D007164:Immunosorbents; D054304:Anti-Mullerian Hormone
keywords: Antimüllerian hormone; antral follicle count; assay