title: Resveratrol inhibits dysfunction of dendritic cells from chronic obstructive pulmonary disease patients through promoting miR-34.
abstract: BACKGROUND
  Resveratrol has demonstrated many beneficial effects against aging, including anti-inflammatory and antioxidant roles. The present study was designed to observe the effects of resveratrol on the dysfunction of dendritic cells (DCs) from COPD patients and its possible mechanism and use in the treatment for COPD.
  
  
  METHODS
  Flow cytometry analysis was used to examine the expression of costimulatory markers CD80 and CD86 and ELISA was used to examine the secretion of IFN-α. Expression of miR-34 was examined by using real-time PCR. Expression vector of miR-34, LV3-miR-34 was also constructed and transfected into DCs to observe the effects on functions of DCs.
  
  
  RESULTS
  The results showed that there was remarkable upregulation of CD80 and CD86 and secretion of cytokines IFN-α in DCs from COPD patients. Resveratrol displayed a dose-dependent cytotoxicity action over 10 µg/mL and pretreatment with resveratrol inhibited upregulation of CD80 and CD86 and secretion of cytokines IFN-α. Further study showed resveratrol upregulated the expression of miR-34, which inhibited the dysfunction of DCs.
  
  
  CONCLUSIONS
  These proofs suggest that resveratrol inhibited dysfunction of DCs from COPD patients through promoting miR-34.
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Guangsu
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Dahe
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Xuzhou Medical College Jiangsu 221006, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Kan
mesh_terms: D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D014162:Transfection; D015854:Up-Regulation
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; C497964:CD86 protein, human; C573415:IFNG protein, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D013267:Stilbenes; D007371:Interferon-gamma; D000077185:Resveratrol
keywords: Resveratrol; chronic obstructive pulmonary disease (COPD); dendritic cells (DCs); miR-34