title: Identification of Teleost Skin CD8α+ Dendritic-like Cells, Representing a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells.
abstract: Although fish constitute the most ancient animal group in which an acquired immune system is present, the presence of dendritic cells (DCs) in teleosts has been addressed only briefly, and the identification of a specific DC subset in teleosts remained elusive because of the lack of specific Abs. In mice, DCs expressing CD8α(+) in lymphoid tissues have the capacity to cross-present extracellular Ags to T cells through MHC I, similarly to tissue-derived CD103(+) DCs and the human CD141(+) DC population. In the current study, we identified a large and highly complex subpopulation of leukocytes coexpressing MHC class II and CD8α. This CD8α(+) MHC II(+) DC-like subpopulation constituted ∼1.2% of the total leukocyte population in the skin, showing phenotypical and functional characteristics of semimature DCs that seem to locally regulate mucosal immunity and tolerance in a species lacking lymph nodes. Furthermore, we identified trout homologs for CD141 and CD103 and demonstrated that, in trout, this skin CD8(+) DC-like subpopulation expresses both markers. To our knowledge, these results provide the first evidence of a specific DC-like subtype in nonimmune tissue in teleosts and support the hypothesis of a common origin for all mammalian cross-presenting DCs.
authors.affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, 28130 Madrid, Spain; and.
authors.forename: Aitor G
authors.initials: AG
authors.lastname: Granja
authors.affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, 28130 Madrid, Spain; and.
authors.forename: Esther
authors.initials: E
authors.lastname: Leal
authors.affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, 28130 Madrid, Spain; and.
authors.forename: Jaime
authors.initials: J
authors.lastname: Pignatelli
authors.affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, 28130 Madrid, Spain; and.
authors.forename: Rosario
authors.initials: R
authors.lastname: Castro
authors.affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, 28130 Madrid, Spain; and.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Abós
authors.affiliation: Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.
authors.forename: Goshi
authors.initials: G
authors.lastname: Kato
authors.affiliation: Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Fischer
authors.affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, 28130 Madrid, Spain; and tafalla@inia.es.
authors.forename: Carolina
authors.initials: C
authors.lastname: Tafalla
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism*; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D005399:Fishes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism; D007801:Langerhans Cells / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis; D010641:Phenotype; D010802:Phylogeny; D020543:Proteome; D040901:Proteomics; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018180:Thrombomodulin; D051193:Toll-Like Receptors / Q000378:metabolism; D059467:Transcriptome; D015054:Zymosan / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D000949:Histocompatibility Antigens Class II; D039001:Integrin alpha Chains; D020543:Proteome; C546931:THBD protein, human; D018180:Thrombomodulin; D051193:Toll-Like Receptors; C091367:alpha E integrins; D015054:Zymosan