title: A simple fluorescent probe based on a pyrene derivative for rapid detection of protamine and monitoring of trypsin activity.
abstract: We report the synthesis of a simple pyrene derivative and its application in protamine detection and monitoring of trypsin activity. This assay can be conducted in aqueous solution and features rapid response, visual detection, high sensitivity and selectivity. The limit of detection of protamine was 0.5 μg mL(-1). The IC50 value of a soybean trypsin inhibitor was estimated to be 0.51 U mL(-1).
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China. yaozy@ihep.ac.cn haichenwu@ihep.ac.cn.
authors.forename: Baiyang
authors.initials: B
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Gefu
authors.initials: G
authors.lastname: Wang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Yao
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hai-Chen
authors.initials: HC
authors.lastname: Wu
mesh_terms: D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D011479:Protamines / Q000032:analysis*; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D013025:Glycine max / Q000201:enzymology; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D011479:Protamines; D011721:Pyrenes; D014361:Trypsin Inhibitors; D014357:Trypsin