title: Molecular genotyping of Echinococcus granulosus using formalin-fixed paraffin-embedded preparations from human isolates in unusual tissue sites.
abstract: Cystic echinococcosis (CE) caused by Echinococcus granulosus remains a serious problem worldwide for issues relating to public health and the economy. The most predominantly affected sites are the liver and the lungs, but other organs such as the heart, the spleen and the peritoneum can also be infected. Access to cysts from uncommon sites has limited genomic and molecular investigations. In the present study, genotypes of E. granulosus sensu lato were identified from formalin-fixed paraffin-embedded tissues (FF-PETs) implicated in human CE. Tissue samples were obtained from 57 patients with histologically confirmed CE. DNA samples were analysed using Egss 1 polymerase chain reaction (PCR) specific to the mitochondrial 12S rRNA gene of E. granulosus sensu stricto. All cysts were typed as E. granulosus sensu stricto with up to 35% of the liver and 16.6% of lungs being the most frequently infected, and up to 48.4% of samples being from rare sites. No correlation was found between cyst site and either the gender or the age of patients. This study demonstrates the possibility of exploiting atypical cysts using FF-PET samples and highlights the predominance of E. granulosus sensu stricto species in the Tunisian population, even in unusual infection sites.
authors.affiliation: LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir,Université de Monastir,Monastir5000,Tunisie.
authors.forename: A
authors.initials: A
authors.lastname: Hizem
authors.affiliation: LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir,Université de Monastir,Monastir5000,Tunisie.
authors.forename: S
authors.initials: S
authors.lastname: M'rad
authors.affiliation: LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir,Université de Monastir,Monastir5000,Tunisie.
authors.forename: M
authors.initials: M
authors.lastname: Oudni-M'rad
authors.affiliation: Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Hached,Sousse4000,Tunisie.
authors.forename: S
authors.initials: S
authors.lastname: Mestiri
authors.affiliation: Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Bourguiba,Monastir5000,Tunisie.
authors.forename: F
authors.initials: F
authors.lastname: Hammedi
authors.affiliation: LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir,Université de Monastir,Monastir5000,Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Mezhoud
authors.affiliation: Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Bourguiba,Monastir5000,Tunisie.
authors.forename: A
authors.initials: A
authors.lastname: Zakhama
authors.affiliation: Laboratoire d'Anatomie et Cytologie Pathologiques, EPS F. Hached,Sousse4000,Tunisie.
authors.forename: M
authors.initials: M
authors.lastname: Mokni
authors.affiliation: LP3M: Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire, LR 12ES08, Département de Biologie Clinique B, Faculté de Pharmacie de Monastir,Université de Monastir,Monastir5000,Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Babba
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D004443:Echinococcosis / Q000469:parasitology* / Q000473:pathology*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics*; D005404:Fixatives; D005557:Formaldehyde; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D010232:Paraffin; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D013048:Specimen Handling / Q000379:methods*; D016707:Tissue Fixation
chemical_list: D018509:DNA, Helminth; D005404:Fixatives; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D005557:Formaldehyde; D010232:Paraffin