title: Streptococcal IdeS: therapeutic potential for Guillain-Barré syndrome.
abstract: Plasma exchange and intravenous immunoglobulin are effective in treating Guillain-Barré syndrome (GBS) probably because the former removes IgG autoantibodies and complement and the latter inhibits complement activation subsequent to the autoantibody binding to peripheral nerve antigens. IgG degrading enzyme of Streptococcus pyogenes (IdeS) can cleave the pathogenic autoantibodies into F(ab')2 and Fc. The purpose of this study is to show whether IdeS has novel therapeutic potential for GBS. Sera with anti-ganglioside IgG antibodies from 15 patients with GBS or Miller Fisher syndrome were used. We tested whether IdeS cleaved the anti-ganglioside IgG antibodies and inhibited deposition of activated complement component on ELISA plates. IdeS efficiently cleaved IgG and blocked complement activation mediated by anti-GM1, anti-GD1a and anti-GQ1b IgG antibodies. IdeS has therapeutic potential for GBS and related conditions.
authors.affiliation: Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Ryo
authors.initials: R
authors.lastname: Takahashi
authors.affiliation: 1] Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore [2] Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yuki
mesh_terms: D001323:Autoantibodies / Q000276:immunology; D001426:Bacterial Proteins / Q000627:therapeutic use*; D003167:Complement Activation / Q000276:immunology; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives / Q000276:immunology; D005732:Gangliosides / Q000276:immunology; D020275:Guillain-Barre Syndrome / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D011485:Protein Binding
chemical_list: D001323:Autoantibodies; D001426:Bacterial Proteins; D005732:Gangliosides; D007074:Immunoglobulin G; C442815:Mac-1-like protein, Streptococcus; C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside; C039129:GQ1b ganglioside