title: Nucleation temperature-controlled synthesis and in vitro toxicity evaluation of L-cysteine-capped Mn:ZnS quantum dots for intracellular imaging.
abstract: Quantum dots (QDs), one of the fastest developing and most exciting fluorescent materials, have attracted increasing interest in bioimaging and biomedical applications. The long-term stability and emission in the visible region of QDs have proved their applicability as a significant fluorophore in cell labelling. In this study, an attempt has been made to explore the efficacy of L-cysteine as a capping agent for Mn-doped ZnS QD for intracellular imaging. A room temperature nucleation strategy was adopted to prepare non-toxic, water-dispersible and biocompatible Mn:ZnS QDs. Aqueous and room temperature QDs with L-cysteine as a capping agent were found to be non-toxic even at a concentration of 1500 µg/mL and have wide applications in intracellular imaging.
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India.|Department of Applied Chemistry, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Pandey
authors.affiliation: Department of Applied Chemistry, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India.
authors.forename: Gajanan
authors.initials: G
authors.lastname: Pandey
authors.affiliation: Developmental Toxicology Laboratory, Systems Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India.
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Tripathi
authors.affiliation: Pesticide Toxicology, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India.
authors.forename: Sapna
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India.|Pesticide Toxicology, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India.|Academy of Scientific and Innovative Research, Council of Scientific & Industrial Research, PO Box 80, MG Marg, Lucknow, 226001, India.
authors.forename: Mohana Krishna Reddy
authors.initials: MKR
authors.lastname: Mudiam
mesh_terms: D003545:Cysteine / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D008345:Manganese / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry* / Q000633:toxicity*; D013440:Sulfides / Q000737:chemistry*; D013696:Temperature; D014407:Tumor Cells, Cultured; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; D003545:Cysteine; C031238:zinc sulfide
keywords: bioimaging; l-cysteine; neuronal cells; nucleation; quantum dots