title: Gold Nanoparticles as Carriers for Synthetic Glycoconjugate Vaccines.
abstract: Recent advances in the preparation and characterization of metal core-based nanoparticles have opened the way to their exploration as carriers for carbohydrate-based vaccines. Here we describe the protocols for the preparation and characterization of water dispersible gold nanoparticles (1-3 nm gold diameter) as carriers for carbohydrate antigens. We mainly refer to the protocols we used for the preparation of gold glyconanoparticles as carrier for an S. pneumoniae carbohydrate-antigen. The high number of ligands at the gold nanoparticles surface and the easiness of their one-pot preparation make these biocompatible nanomaterials an attractive tool for glyco-scientists.
authors.affiliation: Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, Paseo Miramón 182, 20009, San Sebastián, Spain, chiodo.fabrizio@gmail.com.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Chiodo
authors.forename: Marco
authors.initials: M
authors.lastname: Marradi
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D013296:Streptococcus pneumoniae / Q000737:chemistry; D014614:Vaccines, Synthetic / Q000737:chemistry*
chemical_list: D000942:Antigens, Bacterial; D002241:Carbohydrates; D004337:Drug Carriers; D006001:Glycoconjugates; D008024:Ligands; D014614:Vaccines, Synthetic; D006046:Gold