title: Identification of recurrence-related serum microRNAs in hepatocellular carcinoma following hepatectomy.
abstract: Hepatocellular carcinoma (HCC) is one of the most deadly tumors. Prognosis of patients with HCC is generally poor due to the high recurrence rate. In the present study, TaqMan Real-time PCR microRNA Array was used to identify differentially expressed miRNAs from 10 tumor tissue samples (5 from recurrence group vs. 5 from non-recurrence group) and the matched serum samples. Four differentially expressed miRNAs (miR-486-5p, miR-422a, miR-125b and miR-139-5p) were further quantified in 20 tumor tissues and 116 HCC patients' serum before they received hepatectomy. Univariate analysis revealed that miR-486-5p, miR-422a and miR-125b were significantly associated with patients' relapse free survival (RFS). Multivariate analysis demonstrated that miR-486-5p, AFP and microvascular invasion (MVI) were the independent prognostic factors associated with RFS in this cohort (p = 0.000, 0.043, 0.000, respectively). Besides, the expression levels of miR-486-5p were positively correlated in tumor tissues and the paired serum samples, so was miR-422a. The probability of the prognostic accuracy of miR-486-5p in predicting postoperative recurrence of HCC within the first year was 76.79% (65.38% specificity and 81.58% sensitivity), which was almost equal to the classifier established by combination of AFP and MVI (75.98% probability, 63.13% specificity and 85.90% sensitivity). Furthermore, the combination of AFP, MVI and miR-486-5p yielded a ROC curve area of 88.02% (69.20% specificity and 92.10% sensitivity). Our study was the first to identify that serum miR-486-5p could be used to stratify the patients with higher recurrence risk before hepatic resection and potentially guide more effective surveillance strategies for them.
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: b Laboratory of Cell and Molecular Biology & State Key Laboratory of Molecular Oncology ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: b Laboratory of Cell and Molecular Biology & State Key Laboratory of Molecular Oncology ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhu
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Rong
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Songlin
authors.initials: S
authors.lastname: An
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Faqiang
authors.initials: F
authors.lastname: Liu
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: a Department of Abdominal Surgery ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Wu
authors.affiliation: b Laboratory of Cell and Molecular Biology & State Key Laboratory of Molecular Oncology ; Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College ; Beijing , PR China.
authors.forename: Ningzhi
authors.initials: N
authors.lastname: Xu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000601:surgery*; D005260:Female; D006498:Hepatectomy / Q000379:methods*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000601:surgery*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
chemical_list: D035683:MicroRNAs
keywords: RFS; hepatocellular carcinoma (HCC); miR-486–5p; microRNA (miRNA); recurrence