title: High-resolution structures of the D-alanyl carrier protein (Dcp) DltC from Bacillus subtilis reveal equivalent conformations of apo- and holo-forms.
abstract: D-Alanylation of lipoteichoic acids plays an important role in modulating the properties of Gram-positive bacteria cell walls. The D-alanyl carrier protein DltC from Bacillus subtilis has been solved in apo- and two cofactor-modified holo-forms, whereby the entire phosphopantetheine moiety is defined in one. The atomic resolution of the apo-structure allows delineation of alternative conformations within the hydrophobic core of the 78 residue four helix bundle. In contrast to previous reports for a peptidyl carrier protein from a non-ribosomal peptide synthetase, no obvious structural differences between apo- and holo-DltC forms are observed. Solution NMR spectroscopy confirms these findings and demonstrates in addition that the two forms exhibit similar backbone dynamics on the ps-ns and ms timescales.
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther Universität Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Zimmermann
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther Universität Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Pfennig
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther Universität Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany.
authors.forename: Piotr
authors.initials: P
authors.lastname: Neumann
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther Universität Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany.
authors.forename: Huma
authors.initials: H
authors.lastname: Yonus
authors.affiliation: Institut für Physik, Martin-Luther-Universität Halle-Wittenberg, Betty-Heimann-Straße 7, D-06120 Halle/Saale, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Weininger
authors.affiliation: Institut für Physik, Martin-Luther-Universität Halle-Wittenberg, Betty-Heimann-Straße 7, D-06120 Halle/Saale, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Kovermann
authors.affiliation: Institut für Physik, Martin-Luther-Universität Halle-Wittenberg, Betty-Heimann-Straße 7, D-06120 Halle/Saale, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Balbach
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther Universität Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany. Electronic address: stubbs@biochemtech.uni-halle.de.
authors.forename: Milton T
authors.initials: MT
authors.lastname: Stubbs
mesh_terms: D001059:Apoproteins / Q000737:chemistry*; D001412:Bacillus subtilis; D001426:Bacterial Proteins / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C085651:D-alanyl carrier protein, bacteria
keywords: Carrier protein; Dynamics; Lipoteichoic acid biosynthesis; Non-ribosomal peptide synthesis; Posttranslational modification; Structure