title: An investigation of the well-water quality: immunosensor for pathogenic Pseudomonas aeruginosa detection based on antibody-modified poly(pyrrole-3 carboxylic acid) screen-printed carbon electrode.
abstract: In this report, we describe a new immunosensor designed for the detection and the quantification of Pseudomonas aeruginosa bacteria in water. The developed biosensing system was based on the immobilization of purified polyclonal anti P. aeruginosa antibodies on electropolymerized poly(pyrrole-3-carboxylic acid)/glassy carbon electrode. The building of the immunosensor step by step was evaluated by electrochemical measurements such as cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical signature of the immunosensor was established by the change of the charge transfer resistance when the bacteria suspended in solution became attached to the immobilized antibodies. As a result, stable and high sensitive impedimetric immunosensor was obtained with a sensitivity of 0.19 kΩ/decade defined in the linear range from 10(1) to 10(7) CFU/mL of cellular concentrations. A low detection limit was obtained for the P. aeruginosa bacteria and a high selectivity when other bacteria were occasioned as well as Escherichia coli. The developed immunosensor was applied in detecting pathogenic P. aeruginosa in well-water.
authors.affiliation: Laboratory of Analysis, Treatment and Valorisation of Pollutants of Environment and of Products, Faculty of Pharmacy, University of Monastir, 5019, Monastir, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Bekir
authors.affiliation: Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, University of Monastir, Monastir, Tunisia.
authors.forename: Feriel
authors.initials: F
authors.lastname: Bousimma
authors.affiliation: Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, University of Monastir, Monastir, Tunisia.
authors.forename: Houcine
authors.initials: H
authors.lastname: Barhoumi
authors.affiliation: Laboratory of Analysis, Treatment and Valorisation of Pollutants of Environment and of Products, Faculty of Pharmacy, University of Monastir, 5019, Monastir, Tunisia.
authors.forename: Kais
authors.initials: K
authors.lastname: Fedhila
authors.affiliation: Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, University of Monastir, Monastir, Tunisia.
authors.forename: Abderrazak
authors.initials: A
authors.lastname: Maaref
authors.affiliation: Laboratory of Analysis, Treatment and Valorisation of Pollutants of Environment and of Products, Faculty of Pharmacy, University of Monastir, 5019, Monastir, Tunisia.
authors.forename: Amina
authors.initials: A
authors.lastname: Bakhrouf
authors.affiliation: Higher Institute of Applied Sciences and Technology of Mahdia, Monastir University, Monastir, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Ben Ouada
authors.affiliation: Laboratory of Analytical Sciences, Doua, 69100, Villeurbanne, Lyon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Namour
authors.affiliation: Laboratory of Analytical Sciences, Doua, 69100, Villeurbanne, Lyon, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.affiliation: Higher Institute of Applied Sciences and Technology of Mahdia, Monastir University, Monastir, Tunisia. hdbenmansour@gmail.com.
authors.forename: Hedi
authors.initials: H
authors.lastname: Ben Mansour
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D058266:Dielectric Spectroscopy; D060766:Drinking Water / Q000032:analysis / Q000382:microbiology*; D004566:Electrodes; D060587:Groundwater / Q000032:analysis / Q000382:microbiology*; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000302:isolation & purification; D011758:Pyrroles / Q000737:chemistry; D060753:Water Quality
chemical_list: D055910:Antibodies, Immobilized; D002264:Carboxylic Acids; D060766:Drinking Water; D011108:Polymers; D011758:Pyrroles; C488775:poly(pyrrole-3-carboxylic acid); D002244:Carbon
keywords: Glassy carbon electrode; Impedimetric immunosensor; P. aeruginosa; Pathogenic bacteria; Water quality