title: Crosslinked Polypropylenimine Dendrimers With Bioreducible Linkages for Gene Delivery Systems and Their Reductive Degradation Behaviors.
abstract: Crosslinked bioreducible polypropylenimine-cystaminebisacrylamides (PPI-CBAs) were synthesized for gene delivery systems. They formed nano-sized polyplexes with high stability even in reducing condition probably due to the re-crosslinking. PPI-CBAs displayed high transfection efficiency comparable to PEI25k in serum condition. However, cytotoxicity increase, glutathione (GSH) decrease, and reactive oxygen species (ROS) increase were observed in cells with the elevation of PPI-CBAs concentration and crosslinking degree. These results suggest that the cytotoxicity of bioreducible polymers may be closely related with their structures and that reduction of GSH by degradation of re-crosslinked bioreducible polymers and the following increase of ROS may induce cytotoxicity by oxidative stress.
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea.
authors.forename: Gyeong Jin
authors.initials: GJ
authors.lastname: Lee
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea.
authors.forename: Kitae
authors.initials: K
authors.lastname: Ryu
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea.
authors.forename: Kyunghwan
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea.
authors.forename: Ji-Yeong
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea. seal1004@snu.ac.kr.|Research Institute of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea. seal1004@snu.ac.kr.
authors.forename: Tae-Il
authors.initials: TI
authors.lastname: Kim
mesh_terms: D063566:Biodegradable Plastics / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
chemical_list: D063566:Biodegradable Plastics; D011126:Polypropylenes; C443641:poly(propyleneimine)
keywords: bioreducible polymer; crosslink; cytotoxicity; gene delivery system; polypropylenimine