title: Production of adipic acid by the native-occurring pathway in Thermobifida fusca B6.
abstract: OBJECTIVE
  To identify the native-occurring adipate pathway in a previously engineered Thermobifida fusca B6 strain and optimize the adipic acid production of this strain on glucose and corncob.
  
  
  RESULTS
  The native-occurring adipate pathway in T. fusca B6 was identified to be the reverse adipate degradation pathway, including five steps: β-ketothiolase (Tfu_0875), 3-hydroxyacyl-CoA dehydrogenase (Tfu_2399), 3-hydroxyadipyl-CoA dehydrogenase (Tfu_0067), 5-Carboxy-2-pentenoyl-CoA reductase (Tfu_1647) and succinyl-CoA synthetase (Tfu_2576, Tfu_2577). The cell lysates of T. fusca wild-type strain with the addition of the Tfu_1647 protein produced trace adipic acid, while no adipic acid was produced in the absence of this protein. The above results prove that the low expression of Tfu_1647 in the wild-type strain was the reason why it did not produce any adipic acid. We then demonstrated that in T. fusca B6, the maximal titre of adipic acid on 50 g l(-1) glucose was 2·23 g l(-1) with 0·045 g g(-1) -glucose yield and 0·22 g l(-1) adipic acid was produced from 19·38 g l(-1) milled corncob.
  
  
  CONCLUSIONS
  The reverse adipate degradation pathway was found to be responsible for the adipate synthesis in T. fusca B6 and Tfu_1647 was the regulatory node on this pathway.
  
  
  CONCLUSIONS
  Thermobifida fusca B6 was the first reported micro-organism using its native-occurring pathway to accumulate adipic acid. It had the highest reported yield and titre of adipic acid so far.
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, Wuxi, Jiangsu, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, Wuxi, Jiangsu, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D000272:Adipates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D053858:Metabolic Networks and Pathways
chemical_list: D000272:Adipates; D001426:Bacterial Proteins; C029900:adipic acid; D005947:Glucose
keywords: 5-Carboxy-2-pentenoyl-CoA reductase; T. fusca B6; adipic acid; corncob; reverse adipate degradation pathway