title: Puerarin prevents cardiac hypertrophy induced by pressure overload through activation of autophagy.
abstract: This study aimed to explore the effects of puerarin on autophagy in cardiac hypertrophy. Decreased 5'-adenosine monophosphate kinase (AMPK) activity alone with inhibited autophagy could be detected in rats within 3 weeks after aortic banding (AB). Puerarin treatment for 3 weeks in AB rats significantly restored autophagy. Administration of puerarin for 6 weeks effectively restricted cardiomyocyte hypertrophy and apoptosis. In an in vitro study, similar anti-hypertrophy and anti-apoptosis effects of puerarin on isoprenaline-induced H9c2 cells were also observed. After inhibition of autophagy by pretreatment with 3-methyladenine, the protective effects of puerarin were blocked. Further in vivo study demonstrated that puerarin significantly enabled phosphorylation of 5'-AMPK to be activated, subsequently inhibiting expression of the mammalian target of rapamycin (mTOR) target proteins S6 ribosomal protein and 4E-binding protein 1. All these data indicate that puerarin exerts protective effects against cardiomyocyte hypertrophy and apoptosis, partly by restoration of autophagy through AMPK/mTOR-mediated signaling.
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China.
authors.forename: Zhiye
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China.
authors.forename: Caiwen
authors.initials: C
authors.lastname: Ou
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, China. Electronic address: rocenphone@hotmail.com.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Luo
authors.affiliation: Department of Cardiology, Zhu Jiang Hospital of Southern Medical University, China. Electronic address: minshengchengz@163.com.
authors.forename: Minsheng
authors.initials: M
authors.lastname: Chen
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D006332:Cardiomegaly / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D002352:Carrier Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D047908:Intracellular Signaling Peptides and Proteins; D007529:Isoflavones / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D010750:Phosphoproteins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects
chemical_list: D055372:AMP-Activated Protein Kinases; D000225:Adenine; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007529:Isoflavones; D007545:Isoproterenol; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D019893:Ribosomal Protein S6 Kinases; D058570:TOR Serine-Threonine Kinases; C499526:Eif4ebp1 protein, rat; C526109:Anxa3 protein, rat; C025946:3-methyladenine; C546845:mTOR protein, rat; C033607:puerarin
keywords: AMPK; Autophagy; Cardiac hypertrophy; Puerarin