title: Prognostic significance of SOX-1 expression in human hepatocelluar cancer.
abstract: Sex-determining region Y (SRY)-box 1 (SOX1) as a member of the SOX gene superfamily is reported to function as a tumor suppressor in hepatocelluar cancer (HCC). However, the clinicopathological and prognostic significance of SOX-1 expression in HCC is unclear. First, semi-quantitative RT-PCR and Western blot assays were performed to detect the expression of SOX-1 mRNA and protein in 15 paired of HCC tissues and corresponding nontumor tissues. Next, immunohistochemistry was performed to detect SOX-1 protein expression in another 96 cases of HCC tissues, and analyze its correlation with clincopathological factors of patients. Finally, the survival was evaluated by the Kaplan-Meier method and proportional hazards model. Results showed that the expression levels of SOX-1 mRNA and protein in HCC tissues were significantly lower than that in the corresponding nontumor tissues. Statistical analyses indicated that low SOX-1 expression was significantly correlated with higher incidence of venous or lymphatic invasion and advanced TNM stage. Also, patients with high SOX-1 expression showed better overall survival than those with low SOX-1 expression, and multivariate analysis with the Cox proportional hazards indicated that status of SOX-1 expression might be an independent prognostic factor in HCC patients. Collectively, our results indicated that downregulation of SOX-1 was correlated with poor prognosis and tumor development in HCC.
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China ; Department of Internal Medicine III, 86 Hospital of PLA Dangtu, Anhui Province, China.
authors.forename: Jinshu
authors.initials: J
authors.lastname: Lou
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University Nanjing, Jiangsu Province, China.
authors.forename: Long-Bang
authors.initials: LB
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C490906:SOX1 protein, human; D055748:SOXB1 Transcription Factors
keywords: Hepatocelluar cancer (HCC); SOX-1; immunohistochemistry; overall survival; prognosis