title: Molecular clusters size of Puerariae thomsonii radix aqueous decoction and relevance to oral absorption.
abstract: The multi-component system of traditional Chinese medicine (TCM) is very complicated. The clusters are dynamic aggregates whose molecules are held together by hydrogen-bonded, Van der Waals forces or the opposite charges of particles attract each other. In this paper, field emission scanning electron microscopy proved that molecules form clusters in Pueraria thomsonii Benth (Fenge) water decoction. Four kinds of Fenge water decoction, 0.07 g∙mL-1 (F-1), 0.1 g∙mL-1 (F-2), 0.17 g∙mL-1 (F-3), 0.35 g∙mL-1 (F-4); F-1, average diameter of molecular was about 120 nm; F-2, 195 nm; F-3, 256 nm; and F-4, 480 nm. The molecular size was shown to depend on concentration. Rabbits were given equal does of 2.8 g∙kg-1, to perfuse F-1, F-2, F-3, F-4 in volume of 80 mL, 56 mL, 33 mL, 17 mL, respectively. At 0-180 min to collect 2 mL blood from the rabbit ears middle arteries for metabolism fingerprints, the results show the particle size of molecular is smaller, the absorption of drugs is better instead. The acute blood stasis model rats were treatment with Fenge decoction of 1.5 g∙kg-1 for 14 days, the concentrations of Ang II in plasma were significantly lower in F-1 and F-2 groups than those in model group (p < 0.01 or p < 0.05), but there were no significantly difference in F-3 and F-4 groups than those in model group (p > 0.05). Despite the molecular aggregation is a common physical phenomenon, it influence on the kind and amount of molecule per unit volume. Molecules morphology influence on the absorption behavior of drugs in vivo therefore is to have an impact on pharmacological function.
authors.affiliation: The Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China. hujuanketizu_wg@163.com.
authors.forename: Gong
authors.initials: G
authors.lastname: Wang
authors.affiliation: The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. ycm0408@126.com.|The Second People's Hospital of Fujian Province, Fuzhou 350003, China. ycm0408@126.com.|Zhangzhou Health Vocational College, Zhangzhou 363000, China. ycm0408@126.com.
authors.forename: Caimei
authors.initials: C
authors.lastname: Yang
authors.affiliation: The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. hujuanketizu_zk@163.com.|The Second People's Hospital of Fujian Province, Fuzhou 350003, China. hujuanketizu_zk@163.com.
authors.forename: Kuan
authors.initials: K
authors.lastname: Zhang
authors.affiliation: The Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China. huj@fjtcm.edu.cn.|The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. huj@fjtcm.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.affiliation: The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. pws@fjtcm.edu.cn.|The Second People's Hospital of Fujian Province, Fuzhou 350003, China. pws@fjtcm.edu.cn.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Pang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D008516:Medicine, Chinese Traditional; D008855:Microscopy, Electron, Scanning; D029908:Pueraria / Q000737:chemistry*; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D014867:Water
keywords: HPLC fingerprint; Pueraria thomsonii Benth; absorption behavior; molecular aggregates; water decoction