title: Mature brain-derived neurotrophic factor and its receptor TrkB are upregulated in human glioma tissues.
abstract: There are two forms of brain-derived neurotrophic factor (BDNF), precursor of BDNF (proBDNF) and mature BDNF, which each exert opposing effects through two different transmembrane receptor signaling systems, consisting of p75 neurotrophin receptor (p75NTR) and tyrosine receptor kinase B (TrkB). Previous studies have demonstrated that proBDNF promotes cell death and inhibits the growth and migration of C6 glioma cells through p75NTRin vitro, while mature BDNF has opposite effects on C6 glioma cells. It is hypothesized that mature BDNF is essential in the development of malignancy in gliomas. However, histological data obtained in previous studies were unable distinguish mature BDNF from proBDNF due to the lack of specific antibodies. The present study investigated the expression of mature BDNF using a specific sheep monoclonal anti-mature BDNF antibody in 42 human glioma tissues of different grades and 10 control tissues. The correlation between mature BDNF and TrkB was analyzed. Mature BDNF expression was significantly increased in high-grade gliomas, and was positively correlated with the malignancy of the tumor and TrkB receptor expression. The present data have demonstrated that increased levels of mature BDNF contribute markedly to the development of malignancy of human gliomas through the primary BDNF receptor TrkB.
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiong
authors.affiliation: The First People's Hospital of Yunnan, Kunming, Yunnan 650000, P.R. China.
authors.forename: L I
authors.initials: LI
authors.lastname: Zhou
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Yoon
authors.initials: Y
authors.lastname: Lim
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhu
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Li
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China.
authors.forename: Deng-Li
authors.initials: DL
authors.lastname: Fu
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia ; Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan 650101, P.R. China.
authors.forename: Xin-Fu
authors.initials: XF
authors.lastname: Zhou
keywords: brain-derived neurotrophic factor; glioma; human; tyrosine receptor kinase B