title: Biological evaluation of secondary metabolites from the root of Machilus obovatifolia.
abstract: Bioassay-guided fractionation of the root of Machilus obovatifolia led to the isolation of four new lignans, epihenricine B (1), threo-(7'R,8'R) and threo-(7'S,8'S)-methylmachilusol D (2 and 3), and isofragransol A (4), along with 23 known compounds. The compounds were obtained as isomeric mixtures (i.e., 2/3 and 4/20, resp.). The structures were elucidated by spectral analyses. Among the isolates, 1, licarin A (12), guaiacin (14), (±)-syringaresinol (21), and (-)-epicatechin (23) showed ABTS (=2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical-scavenging activity, with SC50 values of 11.7±0.5, 12.3±1.1, 11.0±0.1, 10.6±0.3, and 9.5±0.2 μM in 20 min, respectively. In addition, kachirachirol B (17) showed cytotoxicity against the NCI-H460 cell line with an IC50 value of 3.1 μg/ml.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C.
authors.forename: Shan-Yu
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C.
authors.forename: Horng-Huey
authors.initials: HH
authors.lastname: Ko
authors.affiliation: Division of Biotechnology and Pharmaceutical Research, National Health Research Institute, Miaoli, Taiwan 350, R.O.C.
authors.forename: Shiow-Ju
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C. (I. S. Chen phone: +886-7-3121101 ext. 2191), (H. S. Chang phone: +886-7-3121101 ext. 2664). hschang@kmu.edu.tw.
authors.forename: Hsun-Shuo
authors.initials: HS
authors.lastname: Chang
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C. (I. S. Chen phone: +886-7-3121101 ext. 2191), (H. S. Chang phone: +886-7-3121101 ext. 2664).
authors.forename: Chu-Hung
authors.initials: CH
authors.lastname: Lin
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C. (I. S. Chen phone: +886-7-3121101 ext. 2191), (H. S. Chang phone: +886-7-3121101 ext. 2664). m635013@kmu.edu.tw.
authors.forename: Ih-Sheng
authors.initials: IS
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D019770:Lauraceae / Q000737:chemistry* / Q000378:metabolism; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D061986:MCF-7 Cells; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D017705:Lignans
keywords: Antioxidant activity; Cytotoxic activity; Machilus obovatifolia