title: Aurora kinase A is essential for correct chromosome segregation in mouse zygote.
abstract: Aurora-A kinase (AURKA), a member of the serine/threonine protein kinase family, is involved in multiple steps of mitotic progression. It regulates centrosome maturation, mitotic spindle formation, and cytokinesis. While studied extensively in somatic cells, little information is known about AURKA in the early cleavage mouse embryo with respect to acentrosomal spindle assembly. In vitro experiments in which AURKA was inactivated with specific inhibitor MLN8237 during the early stages of embryogenesis documented gradual arrest in the cleavage ability of the mouse embryo. In the AURKA-inhibited 1-cell embryos, spindle formation and anaphase onset were delayed and chromosome segregation was defective. AURKA inhibition increased apoptosis during early embryonic development. In conclusion these data suggest that AURKA is essential for the correct chromosome segregation in the first mitosis as a prerequisite for normal later development after first cleavage.
authors.affiliation: Institute of Animal Physiology,Slovak Academy of Sciences,Kosice,Slovakia.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kovarikova
authors.affiliation: Institute of Animal Physiology,Slovak Academy of Sciences,Kosice,Slovakia.
authors.forename: Jan
authors.initials: J
authors.lastname: Burkus
authors.affiliation: Institute of Animal Physiology,Slovak Academy of Sciences,Kosice,Slovakia.
authors.forename: Pavol
authors.initials: P
authors.lastname: Rehak
authors.affiliation: Institute of Animal Physiology and Genetics,Academy of Sciences of the Czech Republic,Libechov,Czech Republic.
authors.forename: Adela
authors.initials: A
authors.lastname: Brzakova
authors.affiliation: Institute of Animal Physiology and Genetics,Academy of Sciences of the Czech Republic,Libechov,Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Solc
authors.affiliation: Institute of Animal Physiology,Slovak Academy of Sciences,Soltesovej 4,040 01 Kosice,Slovakia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Baran
mesh_terms: D000818:Animals; D064096:Aurora Kinase A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001381:Azepines / Q000494:pharmacology; D020090:Chromosome Segregation / Q000187:drug effects / Q000502:physiology*; D046149:Embryo Culture Techniques; D005260:Female; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D059008:Time-Lapse Imaging; D015053:Zygote / Q000187:drug effects / Q000502:physiology*
chemical_list: D001381:Azepines; C550258:MLN 8237; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; D064096:Aurora Kinase A
keywords: Apoptosis; Aurora A; MLN8237; Mouse zygote; Spindle