title: SIRT6/NF-κB signaling axis in ginsenoside Rg1-delayed hematopoietic stem/progenitor cell senescence.
abstract: OBJECTIVE
  To investigate the role of SIRT6/NF-κB signaling axis in ginsenoside Rg1-delayed hematopoietic stem/progenitor cell senescence and to provide theoretical and experimental evidence for delaying HSC/HPC senescence pathway.
  
  
  METHODS
  After the separation and purification by immunomagnetic sorting, Sca-1+HSC/HPC was divided into: normal control group; aging group; positive control group; Rg1 delaying group and Rg1 treatment group. Senescence-associated β-galactosidase (SA-β-Gal) staining, flow cytometry analysis of cell cycle and hematopoietic progenitor cells mixed colony (CFU-Mix) culture were performed to determine the delaying or curing roles of Rg1 in Sca-1+HSC/HPC senescence. Quantitative PCR and Western blotting were used to detect the mRNA and protein expression of senescence regulatory molecules, such as SIRT6 and NF-κB.
  
  
  RESULTS
  Compared with the aging group, the positive rate of SA-β-gal staining cells and the proportion of cells in G1 phase decreased; the number of CFU-Mix increased; mRNA and protein expression of SIRT6 increased; mRNA and protein expression of NF-κB was down-regulated in Rg1 delaying and treatment groups; the changes of the indicators in Rg1 delaying group were more significant than those in Rg1 treatment group.
  
  
  CONCLUSIONS
  Rg1 may fight against Sca-1+HSC/HPC senescence induced by t-BHP through regulating SIRT6-NF-κB signaling pathway.
authors.affiliation: Department of Radiology, Affiliated Hospital of Dali University Yunnan 671000, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Tang
authors.affiliation: Department of Histology and Embryology, Dali University Yunnan 671000, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Stem Cell and Tissue Engineering Laboratory, Department of Histology and Embryology, Chongqing Medical University Chongqing 400016, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Wang
authors.affiliation: Stem Cell and Tissue Engineering Laboratory, Department of Histology and Embryology, Chongqing Medical University Chongqing 400016, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Dali University Yunnan 671000, China.
authors.forename: Ji-Chao
authors.initials: JC
authors.lastname: Ding
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D005786:Gene Expression Regulation; D036145:Ginsenosides / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000950:Antigens, Ly; D015415:Biomarkers; D036145:Ginsenosides; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D020122:tert-Butylhydroperoxide; D001616:beta-Galactosidase; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; C035054:ginsenoside Rg1
keywords: Ginsenoside Rg1; NF-Κb; SIRT6; Sca-1+HSC/HPC; senescence