title: Adenovirus-mediated over-expression of Septin4 ameliorates hepatic fibrosis in mouse livers infected with Schistosoma japonicum.
abstract: Septin4 (Sept4) belongs to Septin family and may be involved in apoptosis, vesicle trafficking and other cell processes. In this study, we attempted to investigate the effect of Sept4 in hepatic fibrosis induced by Schistosoma japonicum. ICR mice infected with S. japonicum for 12weeks were treated with PBS, Ad-ctr and Ad-Sept4, respectively. All mice were killed at 2weeks after injection, and the changes in the fibrotic livers were detected via H&E staining, Sirius red staining, qRT-PCR, western blot and TUNEL analysis. In addition, pcDNA3.1-Sept4 plasmid was transfected into LX-2 cells to observe the effect of Sept4 on apoptosis of HSCs in vitro. Ad-Sept4 could ameliorate liver fibrosis, as detected by H&E staining and Sirius red staining. The number of TUNEL-positive cells was increased in the Ad-Sept4 treated group. The expression of Sept4 and cleaved-caspase-3 were all augmented, while the expression of α-SMA, Col1α1 and IL-13 were reduced in the Ad-Sept4 treated group, compared with that expressed in the Ad-ctr group. Over-expression of Sept4 in LX-2 cells could promote apoptosis of LX-2 cells in vitro. In conclusion, Ad-Sept4 can attenuate the development of liver fibrosis induced by S. japonicum through apoptosis.
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China; Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan, Nanjing 210011, Jiangsu, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China; Molecular Biology Lab, Wuxi Center for Disease Control and Prevention, Wuxi 214023, Jiangsu, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China; Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, People's Republic of China. Electronic address: yinongduan@aliyun.com.
authors.forename: Yinong
authors.initials: Y
authors.lastname: Duan
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis; D000090602:Collagen Type I, alpha 1 Chain; D006801:Humans; D020287:In Situ Nick-End Labeling; D018793:Interleukin-13 / Q000096:biosynthesis; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008103:Liver Cirrhosis / Q000235:genetics / Q000469:parasitology / Q000628:therapy*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D060888:Real-Time Polymerase Chain Reaction; D012549:Schistosoma japonicum; D012554:Schistosomiasis japonica / Q000469:parasitology / Q000473:pathology / Q000628:therapy*; D058112:Septins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000199:Actins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D018793:Interleukin-13; C544013:alpha-smooth muscle actin, mouse; D053148:Caspase 3; C115474:Sept4 protein, mouse; D058112:Septins
keywords: Adenovirus; Hepatic fibrosis; Hepatic stellate cells; Schistosoma japonicum; Septin4