title: MiR-199a inhibits the angiogenic potential of endometrial stromal cells under hypoxia by targeting HIF-1α/VEGF pathway.
abstract: We previously reported that miR-199a suppressed the invasiveness of endometrial stromal cells (ESCs) by targeting IkappaB kinase beta (IKKβ). This study was to investigate the role of miR-199a in the angiogenic potential of ESCs under hypoxia. Forced overexpression of miR-199a in ESCs significantly attenuated its angiogenic potential under hypoxia. Moreover, miR-199a down-regulated the expression level of vascular endothelial growth factor-A (VEGF-A) in ESCs under hypoxic conditions. To delineate the mechanism by which miR-199a reduced VEGF-A production, further analysis of the target genes of miR-199a showed that miR-199a targeted both VEGF-A and Hypoxia-inducible factor (HIF)-1α in ESCs. Our findings indicate that miR-199a may attenuate the angiogenic potential of ESCs under hypoxia partly through HIF-1α/VEGF-A pathway suppression. Therefore, miR-199a may play pivotal roles in the pathogenesis of endometriosis and may become a potential therapeutic target of this disease.
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University 1630 Dong Fang Rd., Shanghai 200127, People's Republic of China ; Shanghai Key Laboratory of Gynecologic Oncology, Focus Construction Subject of Shanghai Education Department Shanghai, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University 1630 Dong Fang Rd., Shanghai 200127, People's Republic of China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Lou
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University 1630 Dong Fang Rd., Shanghai 200127, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University 1630 Dong Fang Rd., Shanghai 200127, People's Republic of China ; Shanghai Key Laboratory of Gynecologic Oncology, Focus Construction Subject of Shanghai Education Department Shanghai, People's Republic of China.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University 1630 Dong Fang Rd., Shanghai 200127, People's Republic of China ; Shanghai Key Laboratory of Gynecologic Oncology, Focus Construction Subject of Shanghai Education Department Shanghai, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Di
mesh_terms: D000328:Adult; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation; D004715:Endometriosis / Q000378:metabolism; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D017154:Stromal Cells / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; C531408:mirn199 microRNA, human
keywords: Endometriosis; HIF-1α; VEGF-A; angiogenesis; miR-199a