title: Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation.
abstract: NIN/RPN12 binding protein 1 (NOB1p) encoded by NOB1 has been found to be an essential factor in 26S proteasome biogenesis which participates in protein degradation. However, the functions of NOB1 in non-small cell lung cancer cells are largely unknown. In the present study, lentivirus-mediated NOB1 shRNA transfection in two non-small cell lung cancer cell lines (A549 and H1299) was accomplished, as determined by fluorescence imaging. Downregulation of NOB1 expression was confirmed by real-time PCR and western blotting. NOB1 silencing resulted in a significant decline in the proliferation and colony formation capability of non-small cell lung cancer cells. Moreover, flow cytometry showed that A549 cells were arrested in the G0/G1 phase of the cell cycle after NOB1 suppression. Furthermore, depletion of NOB1 resulted in a significant decrease in CDK4 and cyclin D1 expression. These results suggest that NOB1 may act as an important regulator in non-small cell lung cancer growth and could be a therapeutic target of non‑small cell lung cancer.
authors.affiliation: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Radiology, The Fourth People's Hospital of Jinan, Jinan, Shangdong 250031, P.R. China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Medical Engineering, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.
authors.forename: Benhua
authors.initials: B
authors.lastname: Su
authors.affiliation: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000096:biosynthesis; D051358:Cyclin-Dependent Kinase 4 / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus; D009687:Nuclear Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*
chemical_list: C530104:CCND1 protein, human; C506664:NOB1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D019938:Cyclin D1; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4