title: CXCL12 rs266085 and TNF-α rs1799724 polymorphisms and susceptibility to cervical cancer in a Chinese population.
abstract: Further research is required to identify single nucleotide polymorphisms (SNPs) associated with cervical cancer. The aim of this study was to assess the association of TNF-α/rs1799724 and CXCL12/rs266085 polymorphisms with susceptibility to cervical cancer in Han Chinese population in Shandong Province. 348 patients with cervical squamous cell carcinoma, including CIS (121) and invasive carcinoma (227), and 351 healthy controls. Genomic DNA was isolated from peripheral blood and genotyping for TNF-α/rs1799724 and CXCL12/rs266085 was carried out using TaqMan SNP Genotyping Assays. TNF-α/rs1799724 polymorphism showed the C-allele was less prevalent among cases as compared to controls (74.3% vs. 92.0%), while the T-allele was more prevalent among cases (P=0.000, OR=3.99, 95% C.I.: 2.90-5.51). CXCL12/rs266085 polymorphism showed the C-allele was less prevalent among cases as compared to controls (41.2% vs. 49.7%), while the T-allele was more prevalent among cases (P=0.001, OR=1.41, 95% C.I.: 1.14-1.74). The genotype and allele frequencies of these two SNPs did not differ between CIS and invasive squamous cell carcinoma (P>0.05). Moreover, the allele frequencies of rs1799724 were significantly different between controls without or with HPV infection (P<0.05). Neither the genotype nor allele frequencies of rs266085 were statistically different between HPV-negative and positive controls. TNF-α/rs1799724 and CXCL12/rs266085 polymorphisms are associated with cervical cancer. C->T polymorphism of these two SNPs and HPV infection are linked to high risk for cervical cancer.
authors.affiliation: Department of Obstetrics & Gynecology, Jinan Military General Hospital Jinan, 250031, China.
authors.forename: Geping
authors.initials: G
authors.lastname: Yin
authors.affiliation: Department of Obstetrics & Gynecology, Jinan Military General Hospital Jinan, 250031, China.
authors.forename: Tongyu
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics & Gynecology, Jinan Military General Hospital Jinan, 250031, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Obstetrics & Gynecology, Jinan Military General Hospital Jinan, 250031, China.
authors.forename: Aifang
authors.initials: A
authors.lastname: Wu
authors.affiliation: Department of Obstetrics & Gynecology, Jinan Military General Hospital Jinan, 250031, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Obstetrics & Gynecology, Jinan Military General Hospital Jinan, 250031, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhi
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D054377:Chemokine CXCL12 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology / Q000235:genetics; D016133:Polymerase Chain Reaction; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000235:genetics / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D014409:Tumor Necrosis Factor-alpha
keywords: CXCL12; Cervical cancer; genetic predisposition; polymorphism; susceptibility; tumor necrosis factor-alpha