title: Genotyping of ESBL producing uropathogenic and diarrheagenic Escherichia coli in southeast of Iran.
abstract: BACKGROUND
  The purpose of the present study was the distribution of blaOXA-1, blaPER-1 and blaVEB genes and the genotyping of these genes in extended-spectrum β-lactamases (ESBL) producing uropathogenic and diarrheagenic Escherichia coli isolates.
  
  
  METHODS
  Of 432 isolates, 58 uropathogenic E. coli (UPEC) and 56 diarrheagenic E. coli (DEC) isolates were shown to produce the ESBLs. The isolates were screened to present ESBLs genes by PCR. Pulsed-Field Gel Electrophoresis (PFGE) was used to genotype E. coli strains which possess ESBLs genes.
  
  
  RESULTS
  The blaOXA-1 gene was detected in 17.24% of UPEC isolates and 1.78% of DEC isolates. Of all the isolates studied, none were positive for the blaPER-1 and blaVEB genes. PFGE revealed that the E. coli isolates possessed blaOXA-1 gene comprised three distinct genotypes.
  
  
  CONCLUSIONS
  The current study illustrated high rates of ESBL-resistant phenotypes of E. coli isolates from urine and diarrhea samples in Iran. The blaOXA-1, blaPER-1 and blaVEB genes were found at low frequencies in studied isolates.
authors.forename: Hesam
authors.initials: H
authors.lastname: Alizade
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fallah
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbarpour
authors.affiliation: Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran. alizade.h2000@yahoo.com.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Aflatoonian
authors.forename: Arezou
authors.initials: A
authors.lastname: Taherpour
authors.forename: Hamid
authors.initials: H
authors.lastname: Sharifi
authors.forename: Hossein
authors.initials: H
authors.lastname: Goudarzi
mesh_terms: D003967:Diarrhea / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D007492:Iran; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014556:Urine / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001618:beta-Lactamases