title: MiR-345 suppresses proliferation, migration and invasion by targeting Smad1 in human prostate cancer.
abstract: BACKGROUND
  The roles of dysregulated microRNAs in prostate cancer metastasis are still unknown. In this study, we found that the expression of miR-345 was significantly downregulated in prostate cancer and clinical prostate cancer tissues.
  
  
  RESULTS
  Overexpression of miR-345 in prostate cancer cells suppressed proliferation, migration and invasion. Using nude mice model, we revealed that miR-345 inhibits the growth of prostate cancer cells in vivo and in vitro. Furthermore, we identified and validated Smad1 as a direct target of miR-345. Ectopic expression of Smad1 without its 3'-UTR rescued miR-345-induced cell migration and invasion inhibition.
  
  
  CONCLUSIONS
  Taken together, our data suggest that miR-345 exerts a suppressive effect on prostate cancer proliferation, invasion and migration through downregulation of Smad1.
authors.affiliation: Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China.
authors.forename: Qi-guang
authors.initials: QG
authors.lastname: Chen
authors.affiliation: Department of Diagnostic Radiology, General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Oncology, General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China.
authors.forename: Shu-qi
authors.initials: SQ
authors.lastname: Du
authors.affiliation: Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China.
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Urology, LiaoNing Cancer Hospital and Institute, Shenyang, China.
authors.forename: Guang-yi
authors.initials: GY
authors.lastname: Shan
authors.affiliation: Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China. kongchuize408@163.com.
authors.forename: Chui-ze
authors.initials: CZ
authors.lastname: Kong
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C561417:MIRN345 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C497399:SMAD1 protein, human; D051898:Smad1 Protein
keywords: Prostate cancer; Smad1; miR-345; microRNA