title: Enhancement of the solubility and stability of D-amino acid oxidase by fusion to an elastin like polypeptide.
abstract: An elastin-like polypeptide (ELP) was fused to D-amino acid oxidases (DAAO). ELP-DAAO exhibited a better solubility in aqueous solutions than DAAO, and its enzymatic activity is about 1.6 times that of DAAO. The stability of the proteins was investigated by interacting with urea at various concentrations. The circular dichroism and fluorescence spectra were measured. The results demonstrated that that ELP-DAAO exhibited a much better stability than DAAO, and ELP-DAAO has retained the α-helix content with a high percentage even at a high urea concentration. The results of this work have demonstrated that the ELP tag can be utilized to purify DAAO, in the meantime the solubility and stability of the enzyme are improved.
authors.affiliation: Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Du
authors.affiliation: Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Cuidan
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: fengwei@mail.buct.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
mesh_terms: D002942:Circular Dichroism; D003605:D-Amino-Acid Oxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004549:Elastin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014508:Urea / Q000737:chemistry
chemical_list: D010455:Peptides; D011993:Recombinant Fusion Proteins; D014508:Urea; D004549:Elastin; D003605:D-Amino-Acid Oxidase
keywords: Elastin-like polypeptide; Solubility; Stability; d-Amino acid oxidase