title: Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium.
abstract: The effect of selenium applied as sodium selenite (Na2SeO3) on the cytotoxicity of vanadyl sulphate (VOSO4) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na2SeO3 at low doses (0.5 and 1 μM) can enhance 100 μM VOSO4-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na2SeO3 with VOSO4 and 1 μM Na2SeO3 with VOSO4. Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO4 alone. In turn, a majority of cells co-treated with VOSO4 and 1 μM Na2SeO3 were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.
authors.affiliation: Department of Cell Biology, Institute of Environmental Protection, The John Paul II Catholic University of Lublin, Kraśnicka Ave. 102, 20-718, Lublin, Poland. iglina@kul.lublin.pl.
authors.forename: Iwona
authors.initials: I
authors.lastname: Zwolak
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D008858:Microscopy, Phase-Contrast; D018038:Sodium Selenite / Q000633:toxicity*; D017968:Vanadium Compounds / Q000633:toxicity*
chemical_list: D017968:Vanadium Compounds; C034028:vanadyl sulfate; D018038:Sodium Selenite
keywords: CHO-K1 cells; Cytotoxicity; Interaction; Selenium; Vanadium