title: MiR-21 promoted proliferation and migration in hepatocellular carcinoma through negative regulation of Navigator-3.
abstract: MicroRNA-21 (miR-21) has been well-established and found to be over-expressed in various human cancers and has been associated with hepatocellular carcinoma (HCC) progression. However, the underlying mechanism of miR-21 involvement in the development and progression of HCC remains to be understood. In the present study, we firstly identified that the Navigator-3 (NAV-3) gene as a novel direct target of miR-21. Knock-down of NAV-3 using shRNA can rescue the effects of anti-miR-21 inhibitor in HCC cell lines, whereas re-expression of miR-21 using transfection with miR-21 mimics phenocopied the NAV-3 knock-down model. Additionally, miR-21 levels inversely correlated with NAV-3 both in HCC cells and tissues. Knock-down of NAV-3 promoted both the proliferation and migration in HCC cells. Together, our findings suggest an important role for miR-21 in the progression of HCC, which negatively regulated Navigator-3 in the migration of HCC.
authors.affiliation: The Digestive and Vascural Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, PR China. Electronic address: dr_zpwang@163.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Department of Liver and Biliary Pancreatic Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Department of General Surgery, Branching Hospital of the First People's Hospital of Urumqi, 830000, Xinjiang Uygur Autonomous Region, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
mesh_terms: D063646:Carcinogenesis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008565:Membrane Proteins / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*
chemical_list: C524940:MIRN21 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C464453:NAV3 protein, human; D009419:Nerve Tissue Proteins
keywords: Hepatocellular carcinoma; Migration; Navigator-3; Tumorigenesis; miR-21