title: Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis.
abstract: This review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell. This review aims to introduce microfluidic related techniques for the isolation, trapping and manipulation of a single cell. The major approaches for detection in single-cell analysis are introduced; the applications of single-cell analysis are then summarized. The review concludes with discussions of the future directions and opportunities of microfluidic systems applied in analysis of a single cell.
authors.affiliation: Institute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 300, Taiwan. roxyjay0406@gmail.com.
authors.forename: Shih-Jie
authors.initials: SJ
authors.lastname: Lo
authors.affiliation: Institute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 300, Taiwan. djyao@mx.nthu.edu.tw.
authors.forename: Da-Jeng
authors.initials: DJ
authors.lastname: Yao
mesh_terms: D000818:Animals; D002469:Cell Separation; D006801:Humans; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*
keywords: microfluidic techniques; single-cell analysis; single-cell manipulation