title: Aquaporin-deficient mutant of Synechocystis is sensitive to salt and high-light stress.
abstract: Cyanobacterial aquaporins play an important role in the regulation of various physiological functions: cell volume control, osmotic stress responses, gas exchange. We employed the AqpZ-deficient mutant of Synechocystis to study the role of aquaporins in responses to salt (NaCl) and high light stress. Electron microscopy and paramagnetic resonance revealed that AqpZ-deficient cells are unable to efficiently regulate the cytoplasmic volume under salt stress. Both photosystems (PSII and, especially, PSI) of these cells are more sensitive to NaCl and to high light. Thus, AqpZ of Synechocystis participates in regulation of the photosynthetic activity of PSI and PSII under salt and high-light stress. Our results demonstrate that AqpZ might be necessary for the repair of PSII and PSI after photodamage.
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Sinetova
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Kirill S
authors.initials: KS
authors.lastname: Mironov
authors.affiliation: Institute of Plant Biology, Biological Research Center, Temesvári krt. 62, H-6701 Szeged, P.O. Box 521, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Mustardy
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Shapiguzov
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Bachin
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Suleyman I
authors.initials: SI
authors.lastname: Allakhverdiev
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia. Electronic address: losda@ippras.ru.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Los
mesh_terms: D020346:Aquaporins / Q000172:deficiency*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects; D008027:Light; D009154:Mutation; D010788:Photosynthesis / Q000187:drug effects / Q000528:radiation effects; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000528:radiation effects*; D046939:Synechocystis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects
chemical_list: D020346:Aquaporins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D012965:Sodium Chloride
keywords: Aquaporin; Cyanobacteria; High-light stress; Photosynthesis; Salt stress; Synechocystis