title: Use of FTA technology for detection of Trichomonas gallinae.
abstract: Trichomonas gallinae is the causative agent for avian trichomonosis, which can have important population implications for domestic turkeys, columbids, raptors, and various passeriformes. Continued population surveillance and genotype distribution is needed to elucidate transmission dynamics and prevalence of T. gallinae among free-ranging birds. However, obtaining live cultures for laboratory testing is logistically challenging, limiting the ability to perform surveillance and genotype investigations. In this study, we evaluated non-indicating FTA Elute cards as a potential sampling storage substrate for downstream use in molecular identification of two T. gallinae isolates. Isolate concentrations of 10 or 100 trichomonads/40 μl were inoculated onto a FTA Elute card in triplicate. At each time point (48 h, 2 weeks, and 3 weeks), DNA elution procedures were performed on the cards, and the eluents were analyzed by conventional polymerase chain reaction (PCR) using trichomonad-specific primers. Three PCR-positive samples were detected at 48 h from one isolate; however, all eluents from cards held for 2 and 3 weeks were PCR-negative. Our results suggest that use of FTA Elute cards for nucleic acid storage can lead to low PCR sensitivity of T. gallinae in low concentrations, such as those found in non-clinical birds; however, more research is needed to fully evaluate the efficacy of FTA Elute cards as a diagnostic tool for T. gallinae.
authors.affiliation: College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Holt
authors.affiliation: Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Purple
authors.affiliation: Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA. Electronic address: rgerhold@utk.edu.
authors.forename: Richard
authors.initials: R
authors.lastname: Gerhold
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D009696:Nucleic Acids; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D014244:Trichomonas / Q000302:isolation & purification*; D014245:Trichomonas Infections / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan; D009696:Nucleic Acids
keywords: Avian; Molecular sampling; Trichomonas gallinae; Trichomonosis