title: Bioreducible Poly-L-Lysine-Poly[HPMA] Block Copolymers Obtained by RAFT-Polymerization as Efficient Polyplex-Transfection Reagents.
abstract: Polylysine-b-p[HPMA] block copolymers containing a redox-responsive disulfide bond between both blocks are synthesized by RAFT polymerization of pentafluorphenyl-methacrylate with a macro-CTA from Nϵ-benzyloxycarbonyl (Cbz) protected polylysine (synthesized by NCA polymerization). This polylysine-b-p[PFMA] precursor block copolymer is converted to polylysine(Cbz)-b-p[HPMA] by postpolymerization modification with 2-hydroxypropylamine. After removal of the Cbz protecting group, cationic polylysine-b-p[HPMA] copolymers with a biosplittable disulfide moiety became available, which can be used as polymeric transfection vectors. These disulfide linked polylysine-S-S-b-p[HPMA] block copolymers show low cytotoxicity and increased transfection efficiencies (HEK-293T cells) compared to analogous blockcopolymers without disulfide group making them interesting for the transfection of sensitive immune cells.
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Kristof
authors.initials: K
authors.lastname: Tappertzhofen
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128, Mainz, Germany.|MAINZ Graduate School of Excellence (Materials Science in Mainz), Johannes Gutenberg-University, Staudingerweg 9, 55128, Mainz, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Beck
authors.affiliation: Department of Dermatology, University Medical Center of the Johannes Gutenberg-University, Langenbeckstrasse 1, 55131, Mainz, Germany.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Montermann
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Huesmann
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Barz
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors.forename: Kaloian
authors.initials: K
authors.lastname: Koynov
authors.affiliation: Department of Dermatology, University Medical Center of the Johannes Gutenberg-University, Langenbeckstrasse 1, 55131, Mainz, Germany. mbros@uni-mainz.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Bros
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128, Mainz, Germany. zentel@uni-mainz.de.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Zentel
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D011107:Polylysine / Q000031:analogs & derivatives / Q000138:chemical synthesis; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis; D014162:Transfection / Q000379:methods*
chemical_list: D011108:Polymers; D011109:Polymethacrylic Acids; C000611745:polylysine-b-poly(2-hydroxypropyl methacrylamide) block copolymer; D011107:Polylysine
keywords: RAFT-polymerization; bioreducible polyplexes; disulfide block copolymers; transfection