title: Transepithelial transport of phenolic acids in Flos Lonicerae Japonicae in intestinal Caco-2 cell monolayers.
abstract: The oral bioavailabilities of phenolic acids in Flos Lonicerae Japonicae beverage were low. The observation from an in vitro Caco-2 cell model showed that the absorptions of phenolic acids were mainly permeated via paracellular diffusion, and influenced by P-glycoprotein (P-gp), multidrug resistance-associated protein 2 (MRP2) and breast cancer resistance protein (BCRP). Besides, the Papp (AP→BL) values in Flos Lonicerae Japonicae were significantly higher than those of monomers, which was attributed to the decrease of efflux ratios (<1.0) influenced by flavones (luteoloside and luteolin) on the P-gp, but they were still poorly absorbed. The results indicated that the absorptions in Flos Lonicerae Japonicae as well as those of monomers were mainly restricted by the tight junctions (TJs). Food supplements (honey and propolis) or edible excipient (chitooligosaccharide) as TJ enhancers will be investigated to improve the functions of Flos Lonicerae Japonicae healthy beverages.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China. diliuqing@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Jinjun
authors.initials: J
authors.lastname: Shan
authors.forename: Shouchuan
authors.initials: S
authors.lastname: Wang
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.forename: Liuqing
authors.initials: L
authors.lastname: Di
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D001692:Biological Transport; D018938:Caco-2 Cells; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D062385:Hydroxybenzoates / Q000493:pharmacokinetics*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry / Q000166:cytology; D007700:Kinetics; D031239:Lonicera / Q000378:metabolism*; D008954:Models, Biological; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C000715087:ABCC2 protein, human; C017616:phenolic acid