title: Evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines.
abstract: Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC. In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. Additionally, interactomic studies were carried out to evaluate the ability of these down- and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes.
authors.affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università degli Studi di Napoli, 80138 Napoli, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Guariniello
authors.affiliation: Dipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, 80138 Napoli, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Bernardo
authors.affiliation: Servizio di Informatica Medica, Azienda Ospedaliera Universitaria, Seconda Università di Napoli, 80138 Napoli, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Colonna
authors.affiliation: Dipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, 80138 Napoli, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Cammarota
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Castello
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, Italy.
authors.forename: Susan
authors.initials: S
authors.lastname: Costantini
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D051140:Selenoproteins / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D051140:Selenoproteins