title: Saliva-Based Screening of High-Risk Human Papillomavirus Strains: Detection in Female Indonesian and Thai Dental Students.
abstract: BACKGROUND
  Currently it is believed that human papillomaviruses (HPV) are associated with the development of some oral/oropharyngeal cancers. It has been suggested that these viruses influence carcinogenesis in both smokers and non-smokers. Data on the prevalence of HPV in healthy adults are thus needed to estimate the risk of oral/oropharyngeal cancer. The aim of this study was to assess the prevalence of oral HPV in healthy female adults in Indonesia and Thailand.
  
  
  METHODS
  Healthy female students from the Faculties of Dentistry of Universitas Indonesia and Chiang Mai University were asked to participate in this pilot study. DNA was extracted from saliva specimens and screened for HPV16 and HPV18 using PCR.
  
  
  RESULTS
  The age, marital status and sexual experience of the subjects between the two countries were not significantly different. Eight (4%) and 4 (2%) samples were positive for HPV16 and HPV18, respectively. Fisher's Exact test found a significant difference between HPV16 positivity in subjects who were married and had sexual intercourse but not for HPV18.
  
  
  CONCLUSIONS
  This study successfully detected presence of HPV16 and HPV18 DNA in a number of saliva samples from female dental school students. Marital status, experience of sexual intercourse and safe sexual practice are related to the possibility of finding HPV DNA finding in saliva. Dentists, physicians and other health care professionals may gain significant value from the findings of this study, which provide an understanding of the nature of HPV infection and its risk to patient health and disease.
authors.affiliation: Department of Oral Medicine, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia E-mail : yuniardini@ui.ac.id.
authors.forename: Yuniardini Septorini
authors.initials: YS
authors.lastname: Wimardhani
authors.forename: Harum
authors.initials: H
authors.lastname: Sasanti
authors.forename: Indriasti Indah
authors.initials: II
authors.lastname: Wardhany
authors.forename: Afi Savitri
authors.initials: AS
authors.lastname: Sarsito
authors.forename: Siti Aliyah
authors.initials: SA
authors.lastname: Pradono
authors.forename: Gus Permana
authors.initials: GP
authors.lastname: Subita
authors.forename: Anandina Irmagita
authors.initials: AI
authors.lastname: Soegyanto
authors.forename: Febrina
authors.initials: F
authors.lastname: Rahmayanti
authors.forename: Nutchapon
authors.initials: N
authors.lastname: Chamusri
authors.forename: Anak
authors.initials: A
authors.lastname: Iamaroon
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008403:Mass Screening; D009367:Neoplasm Staging; D009959:Oropharyngeal Neoplasms / Q000175:diagnosis* / Q000821:virology; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011379:Prognosis; D012463:Saliva / Q000821:virology*; D013335:Students, Dental / Q000706:statistics & numerical data*; D013785:Thailand / Q000453:epidemiology; D055815:Young Adult
chemical_list: D004279:DNA, Viral