title: [Molecularly imprinted polymers in electro analysis of proteins].
abstract: In the review the main approaches to creation of recognition materials capable of competing with biological specific receptors, (polymeric analogs of antibodies or molecularly imprinted polymers, MIP) for the electro analysis of functionally significant proteins such as a myoglobin, troponin T, albumin, human ferritin, calmodulin are considered. The main types of monomers for MIP fabrication, and methods for MIP/protein interactions, such as a surface plasmon resonance (SPR), nanogravimetry with use of the quartz crystal resonator (QCM), spectral and electrochemical methods are discussed. Experimental data on electrochemical registration of a myoglobin using MIP/electrode are presented. For a development of electrochemical sensor systems based on MIPs, o-phenylenediamine (1,2-diaminobenzene was used as a monomer. It was shown that the imprinting factor Imax(MIP)/Imax(NIP), calculated as a myoglobin signal ratio when embedding in MIP to a myoglobin signal when embedding in the polymer received without molecular template (NIP) corresponds 2-4.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia; IBMC-EcoBioPharm Company, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Shumyantseva
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia; IBMC-EcoBioPharm Company, Moscow, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Bulko
authors.affiliation: IBMC-EcoBioPharm Company, Moscow, Russia.
authors.forename: I Kh
authors.initials: IKh
authors.lastname: Baychorov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D058525:Quartz Crystal Microbalance Techniques; D020349:Surface Plasmon Resonance
chemical_list: D011108:Polymers; D011506:Proteins
keywords: MIP sensor; electroanalysis; electrochemical sensors; molecularly imprinted polymers (MIP); molecularly imprinting; nanomaterials; proteins