title: Screening of antioxidant activity and volatile compounds composition of Chamerion angustifolium (L.) Holub ecotypes grown in Lithuania.
abstract: Since biological activity of medicinal plants is dependent on cultivation area, climatic conditions, developmental stage, genetic modifications and other factors, it is important to study flora present in different growing sites and geographical zones. This study was focused on screening of antioxidant activity of C. angustifolium harvested in six different locations in Lithuania. The total contents of phenolic compounds, flavonoids and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were evaluated by spectrophotometric methods. A correlation between radical scavenging activity and total phenolic compounds content was observed (correlation coefficient 0.98). HPLC with online post-column DPPH radical scavenging reaction detection was used for the separation of extracts. Oenothein B, rutin and one unidentified compound were predominant. Volatile compounds were analysed using solid-phase microextraction coupled with gas chromatography-mass spectrometry. Based on the analysis of volatiles, all samples were classified into two chemotypes: (I) with predominant α- and β-caryophyllenes and (II) with predominant anethole.
authors.affiliation: a Faculty of Natural Sciences, Department of Biology , Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Vilma
authors.initials: V
authors.lastname: Kaškonienė
authors.affiliation: a Faculty of Natural Sciences, Department of Biology , Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Audrius
authors.initials: A
authors.lastname: Maruška
authors.affiliation: a Faculty of Natural Sciences, Department of Biology , Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Ieva
authors.initials: I
authors.lastname: Akuņeca
authors.affiliation: a Faculty of Natural Sciences, Department of Biology , Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Mantas
authors.initials: M
authors.lastname: Stankevičius
authors.affiliation: b Sector of Medicinal Plants , Kaunas Botanical Garden of Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Ona
authors.initials: O
authors.lastname: Ragažinskienė
authors.affiliation: a Faculty of Natural Sciences, Department of Biology , Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Violeta
authors.initials: V
authors.lastname: Bartkuvienė
authors.affiliation: a Faculty of Natural Sciences, Department of Biology , Vytautas Magnus University , Kaunas , Lithuania.
authors.forename: Olga
authors.initials: O
authors.lastname: Kornyšova
authors.affiliation: c Faculty of Pharmacy , Lithuanian University of Health Sciences , Kaunas , Lithuania.
authors.forename: Vitalis
authors.initials: V
authors.lastname: Briedis
authors.affiliation: d Institute of Oncology , Lithuanian University of Health Sciences , Kaunas , Lithuania.
authors.forename: Rasa
authors.initials: R
authors.lastname: Ugenskienė
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical / Q000379:methods; D060146:Ecotype; D005419:Flavonoids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008097:Lithuania; D029594:Onagraceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D055549:Volatile Organic Compounds
keywords: Chamerion angustifolium (L.) Holub; SPME-GC-MS; online HPLC-DPPH; radical scavenging activity; total flavonoids content; total phenolic content