title: Mitochondrial oxidative stress-induced hepatocyte apoptosis reflects increased molybdenum intake in caprine.
abstract: Molybdenum (Mo) is an essential trace element for animals and humans. However, the high dietary intake of Mo leads to disease conditions in heavy metal pollution areas. To the best of our knowledge, the effect of high levels of Mo on the apoptosis of hepatocyte in goats has not been investigated. Therefore, the aim of the present in vivo study was to investigate the impact of Mo on mitochondrial oxidative stress and apoptosis genes in the liver using real-time quantitative polymerase chain reaction (RT-qPCR) and transmission electron microscopy, respectively. Thirty-six healthy goats were randomly divided into three groups: two groups treated with ammonium molybdate [(NH4)6·Mo7O24·H2O] at 15 and 45 mg Mo kg(-1) BW, respectively, and a control group without treatment. Liver samples were collected from individual goats at different time intervals. The levels of oxidative stress in the mitochondrial membrane and expression of liver-related apoptosis genes, including Bcl-2, Cyt c, caspase-3, and Smac, were examined. The results demonstrated that the levels of superoxide dismutase (SOD) and catalase (CAT) expression were significantly down-regulated in liver cells, whereas malondialdehyde (MDA), nitric oxide (NO), and total nitric oxide synthase (T-NOS) expression was up-regulated (P < 0.01). The expression of Smac, Cyt c, and caspase-3 was significantly up-regulated, whereas Bcl-2 expression was down-regulated in liver cells (P < 0.01). In addition, histopathological examination revealed varying degrees of vacuolization, irregularity, nuclear fission, and mitochondrial swelling and high-density electrons in the cytoplasm of hepatocytes in groups treated with 15 and 45 mg Mo kg(-1) BW. Thus, these results suggested that high molybdenum induced hepatocyte apoptosis and might involve a mitochondrial pathway.
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China.
authors.forename: Liqi
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Luo
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China.
authors.forename: Caiying
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China. hgljx@163.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, JiangXi Agriculture University, N.O. 1101, Zhimin Avenue, Nanchang Economic and Technological Development District, Nanchang, 330045, People's Republic of China. chbin20020804@163.com.
authors.forename: Huabin
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis; D006041:Goats; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008982:Molybdenum / Q000008:administration & dosage*; D018384:Oxidative Stress
chemical_list: D008982:Molybdenum
keywords: Apoptosis; Goat; Liver; Mitochondria; Molybdenum