title: Usefulness of pulsed-field gel electrophoresis profiles for the determination of Salmonella serovars.
abstract: We created a database consisting of a large number of Salmonella pulsed-field gel electrophoresis (PFGE) profiles covering a wide range of different serovars. This database was used for the prediction of the serovars based on the PFGE profiles for isolates from Taiwan and Denmark. The PFGE profiles proved very useful in the determination of a serovar although serovar prediction was more efficient for local isolates than those from a distant geographic area. To use a highly stringent band matching tolerance in the BioNumerics software is also important for the grouping of serovars.
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: nipmcsc@cdc.gov.tw.
authors.forename: Chien-Shun
authors.initials: CS
authors.lastname: Chiou
authors.affiliation: Statens Serum Institut, DK-2300 Copenhagen S, Denmark. Electronic address: MTD@ssi.dk.
authors.forename: Mia
authors.initials: M
authors.lastname: Torpdahl
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: yingshu0439@cdc.gov.tw.
authors.forename: Ying-Shu
authors.initials: YS
authors.lastname: Liao
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: chunsin@cdc.gov.tw.
authors.forename: Chun-Hsing
authors.initials: CH
authors.lastname: Liao
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: tasur@cdc.gov.tw.
authors.forename: Chi-Sen
authors.initials: CS
authors.lastname: Tsao
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: Judy670310@cdc.gov.tw.
authors.forename: Shiu-Yun
authors.initials: SY
authors.lastname: Liang
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: pleione@cdc.gov.tw.
authors.forename: You-Wun
authors.initials: YW
authors.lastname: Wang
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: kuojc@cdc.gov.tw.
authors.forename: Jung-Che
authors.initials: JC
authors.lastname: Kuo
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: current788@cdc.gov.tw.
authors.forename: Yen-Yi
authors.initials: YY
authors.lastname: Liu
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods* / Q000592:standards*; D003718:Denmark; D016521:Electrophoresis, Gel, Pulsed-Field; D012475:Salmonella / Q000145:classification* / Q000235:genetics*; D065288:Serogroup; D013624:Taiwan
keywords: Molecular serotyping; Pulsed-field gel electrophoresis; Salmonella