title: Controlling the Crystallinity of Thermoresponsive Poly(2-oxazoline)-Based Nanolayers to Cell Adhesion and Detachment.
abstract: Semicrystalline, thermoresponsive poly(2-isopropyl-2-oxazoline) (PIPOx) layers covalently bonded to glass or silica wafers were obtained via the surface-termination of the living polymer chains. Polymer solutions in acetonitrile were exposed to 50 °C for various time periods and were poured onto the functionalized solid wafers. Fibrillar crystallites formed in polymerization solutions settled down onto the wafers next to the amorphous polymer. The amount of crystallites adsorbed on thermoresponsive polymer layers depended on the annealing time of the PIPOx solution. The wettability of PIPOx layers decreased with the increasing amount of crystallites. The higher content of crystallites weakened the temperature response of the layer, as evidenced by the philicity and thickness measurements. Semicrystalline thermoresponsive PIPOx layers were used as biomaterials for human dermal fibroblasts (HDFs) culture and detachment. The presence of crystallites on the PIPOx layers promoted the proliferation of HDFs. Changes in the physicochemical properties of the layer, caused by the temperature response of the polymer, led to the change in the cells shape from a spindle-like to an ellipsoidal shape, which resulted in their detachment. A supporting membrane was used to assist the detachment of the cells from PIPOx biosurfaces and to prevent the rolling of the sheet.
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie -Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Oleszko
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie -Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wałach
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie -Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Utrata-Wesołek
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie -Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kowalczuk
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie -Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Trzebicka
authors.affiliation: Center for Burn Treatment, Jana Pawla II, 41-100 Siemianowice Slaskie, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Klama-Baryła
authors.affiliation: Center for Burn Treatment, Jana Pawla II, 41-100 Siemianowice Slaskie, Poland.
authors.forename: Diana
authors.initials: D
authors.lastname: Hoff-Lenczewska
authors.affiliation: Center for Burn Treatment, Jana Pawla II, 41-100 Siemianowice Slaskie, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Kawecki
authors.affiliation: Department of General, Molecular Biology and Genetics, Medical University of Silesia , Medykow 18, 40-752 Katowice, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Lesiak
authors.affiliation: Department of General, Molecular Biology and Genetics, Medical University of Silesia , Medykow 18, 40-752 Katowice, Poland.
authors.forename: Aleksander L
authors.initials: AL
authors.lastname: Sieroń
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie -Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dworak
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D010080:Oxazoles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D010080:Oxazoles; C577913:poly(2-oxazoline); D012822:Silicon Dioxide