title: Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury.
abstract: Long noncoding RNAs (LncRNAs) have been believed to be the major transcripts in various tissues and organs, and may play important roles in regulation of many biological processes. The current study determined the LncRNA profile in mouse plasma after liver ischemia/reperfusion injury (IRI) using microarray technology. Microarray assays revealed that 64 LncRNAs were upregulated, and 244 LncRNAs were downregulated in the plasma of liver IRI mouse. Among these dysregulated plasma LncRNAs, 59-61% were intergenic, 22-25% were antisense overlap, 8-12% were sense overlap and 6-7% were bidirectional. Ten dysregulated plasma LncRNAs were validated by quantitative PCR assays, confirming the accuracy of microarray analysis result. Comparison analysis between dysregulated plasma and liver LncRNA profile after liver IRI revealed that among the 308 dysregulated plasma LncRNAs, 245 LncRNAs were present in the liver, but remained unchanged. In contrast, among the 98 dysregulated liver LncRNAs after IRI, only 19 were present in the plasma, but remained unchanged. LncRNA AK139328 had been previously reported to be upregulated in the liver after IRI, and silencing of hepatic AK139328 ameliorated liver IRI. Both microarray and RT-PCR analyses failed to detect the presence of AK139328 in mouse plasma. In summary, the current study compared the difference between dysregulated LncRNA profile in mouse plasma and liver after liver IRI, and suggested that a group of dysregulated plasma LncRNAs have the potential of becoming novel biomarkers for evaluation of ischemic liver injury.
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Yanjin
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; Department of Biomedical Informatics, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Junpei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; Department of Biomedical Informatics, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tu
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China; Center for Noncoding RNA Medicine, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Geng
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; Department of Biomedical Informatics, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; Institute of Systems Biomedicine, Peking University, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China; Center for Noncoding RNA Medicine, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Cui
authors.affiliation: Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Beijing, 100191, China; MOE Key Lab of Molecular Cardiovascular Science, Peking University, 38 Xueyuan Road, Beijing, 100191, China; Center for Noncoding RNA Medicine, Peking University Health Science Center, Beijing, 100191, China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000097:blood*; D015427:Reperfusion Injury / Q000097:blood* / Q000473:pathology
chemical_list: D062085:RNA, Long Noncoding