title: Extracellular aromatic biosurfactant produced by Tsukamurella pseudospumae and T. spumae during growth on n-hexadecane.
abstract: Biosurfactants are surface-active agents produced by microorganisms and show increasing significance in various industrial applications. A great variety of these secondary metabolites are described to occur within actinomycetes, amongst trehalose lipids and oligosaccharide lipids produced by the family Tsukamurellaceae. This study reports on the production of not yet described compounds with surface active behavior by non-pathogenic Tsukamurella pseudospumae and Tsukamurella spumae during growth on hydrophobic carbon sources. Extracts of the purified compounds differ in terms of structure and performance properties to other biosurfactants described within their family. Infrared and nuclear magnetic resonance spectroscopic analysis revealed the presence of aromatic moieties within the surfactant produced, which to date is only known to occur within phenolic glycolipids of some mycobateria.
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131 Karlsruhe, Germany. Electronic address: johannes.kuegler@kit.edu.
authors.forename: Johannes H
authors.initials: JH
authors.lastname: Kügler
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131 Karlsruhe, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Kraft
authors.affiliation: Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Heißler
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber Weg 6, 76131 Karlsruhe, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Muhle-Goll
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber Weg 6, 76131 Karlsruhe, Germany; Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Luy
authors.affiliation: Institute of Food Chemistry, University of Hohenheim, Garbenstr. 28, 70599 Stuttgart, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schwack
authors.affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131 Karlsruhe, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Syldatk
authors.affiliation: Institute of Food Science and Biotechnology, Section Bioprocess Engineering, University of Hohenheim, Garbenstr. 25, 70599 Stuttgart, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hausmann
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D000473:Alkanes / Q000494:pharmacology*; D018533:Biomass; D002855:Chromatography, Thin Layer; D005110:Extracellular Space / Q000737:chemistry*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000473:Alkanes; D006841:Hydrocarbons, Aromatic; D013501:Surface-Active Agents; C007932:n-hexadecane
keywords: Actinobacteria; Actinomycete; Bioemulsifier; Biosurfactant; Tsukamurella