title: Analysis of Short-Term Cortisol Stress Response in Channel Catfish by Anesthetization with Metomidate Hydrochloride and Tricaine Methanesulfonate.
abstract: The ability of the anesthetics metomidate hydrochloride and tricaine methanesulfonate (MS-222) to mitigate the cortisol stress response of Channel Catfish Ictalurus punctatus was evaluated during a 10-min confinement stress. The cortisol concentrations of Channel Catfish anesthetized in metomidate hydrochloride remained consistent throughout the 10-min exposure; however, for fish anesthetized with MS-222 and nonanesthetized fish, cortisol concentrations were approximately 7- and 22-fold higher, respectively, than the baseline concentrations. While both anesthetics reduced cortisol concentrations relative to those of nonanesthetized fish, these results suggest that MS-222 is an appropriate anesthetic to use during the initial 5 min of sedation and that metomidate hydrochloride is appropriate for longer periods of sedation.
authors.affiliation: a Ecological Research Center , University of Memphis , Building 8, South Campus, Memphis , Tennessee 38152 , USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Smith
authors.forename: N J
authors.initials: NJ
authors.lastname: Booth
authors.forename: B C
authors.initials: BC
authors.lastname: Peterson
authors.forename: W S
authors.initials: WS
authors.lastname: Stephens
authors.forename: C A
authors.initials: CA
authors.lastname: Goudie
authors.forename: B A
authors.initials: BA
authors.lastname: Simco
mesh_terms: D062365:Aminobenzoates / Q000009:adverse effects / Q000494:pharmacology*; D000777:Anesthetics / Q000009:adverse effects / Q000494:pharmacology; D000818:Animals; D005045:Etomidate / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000494:pharmacology; D006854:Hydrocortisone / Q000097:blood*; D007059:Ictaluridae / Q000097:blood / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D062365:Aminobenzoates; D000777:Anesthetics; C003636:tricaine; D006854:Hydrocortisone; C084586:metomidate; D005045:Etomidate