title: Specific Inhibition of β-Catenin in Jeko-1 Mantle Cell Lymphoma Cell Line Decreases Proliferation and Induces Apoptosis.
abstract: BACKGROUND
  The canonical Wnt signaling pathway has been considered as a potent oncogenic signaling in the initiation and progression of hematological malignancies. As a key regulator of the Wnt signaling pathway, the role of β-catenin in mantle cell lymphoma (MCL) pathogenesis and progression was investigated in this study.
  
  
  METHODS
  A total of 30 MCL samples were collected from patients and were examined for the expression of β-catenin and p-GSK3β using immunohistochemical (IHC) staining. Further in vitro studies employed MTT and Western blot assays detecting proliferation and apoptosis-related proteins in MCL cell line Jeko-1, which were transfected with β-catenin shRNA or specific inhibitor XAV939.
  
  
  RESULTS
  Expression of β-catenin and phosphorylated glycogen synthase kinase-3 beta (p-GSK3β) in MCL was significantly higher than those in controlled samples. In vitro studies indicated that β-catenin knockdown significantly inhibited cell proliferation and induced apoptosis in Jeko-1 cells. Furthermore, XAV939 induced apoptosis and growth arrest in Jeko-1 cells. Both inhibitory agents increased Bax and caspase 3 proteins, and decreased Bcl-2, c-Myc, and Cyclin D1 proteins.
  
  
  CONCLUSIONS
  The specific inhibition of β-catenin induces apoptosis and growth arrest, making it a potential therapeutic target against MCL.
authors.affiliation: Department of Pediatrics, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian, China (mainland).
authors.forename: Jinshui
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Hematology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian, China (mainland).
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian, China (mainland).
authors.forename: Jianmin
authors.initials: J
authors.lastname: Weng
authors.affiliation: Department of Pediatrics, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian, China (mainland).
authors.forename: Liyun
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Pediatrics, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian, China (mainland).
authors.forename: Kaizhi
authors.initials: K
authors.lastname: Weng
authors.affiliation: Department of Hematology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian, China (mainland).
authors.forename: Xudong
authors.initials: X
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051176:beta Catenin / Q000037:antagonists & inhibitors*
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; C544261:XAV939; D051176:beta Catenin