title: Isolation and identification of polyphenols from Marsilea quadrifolia with antioxidant properties in vitro and in vivo.
abstract: Marsilea quadrifolia is an edible aquatic medicinal plant used as a traditional health food in Asia. Four new polyphenols including kaempferol 3-O-(2″-O-E-caffeoyl)-β-d-glucopyranoside (1), kaempferol 3-O-(3″-O-E-caffeoyl)-α-l-arabinopyranoside (3), 4-methy-3'-hydroxypsilotinin (4) and (±)-(E)-4b-methoxy-3b,5b-dihydroxyscirpusin A (18) together with 14 known ones (2, 5-17) were isolated from the ethanol extract of M. quadrifolia. Structures of the new compounds were elucidated by extensive spectroscopic analyses. In DPPH and oxygen radical absorbance capacity antioxidant assays, some compounds showed stronger antioxidant activities and quercetin (9) was the most potent antioxidant in both assays. In a restraint-induced oxidative stress model in mice, quercetin significantly attenuated the increase in plasma ALT and AST levels as well as liver MDA content of restrained mice. Liver SOD activity was also significantly increased by quercetin, indicating a significant in vivo antioxidant activity. As a rich source of polyphenols with strong antioxidant activities, M. quadrifolia may be developed to a product for relieving oxidative stress.
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Tian
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Tan
authors.affiliation: b State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine , The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong SAR , P.R. China.
authors.forename: Yuk-Lau
authors.initials: YL
authors.lastname: Wong
authors.affiliation: c School of Life Sciences , The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong SAR , P.R. China.
authors.forename: Hoi Yan
authors.initials: HY
authors.lastname: Wu
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Jia
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Guo-En
authors.initials: GE
authors.lastname: Wang
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Gao
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Li
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kurihara
authors.affiliation: b State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine , The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong SAR , P.R. China.|c School of Life Sciences , The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong SAR , P.R. China.
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
authors.affiliation: a College of Pharmacy, Jinan University , Guangzhou 510632 , P.R. China.
authors.forename: Ren-Wang
authors.initials: RW
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001208:Asia; D004353:Drug Evaluation, Preclinical / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D043964:Marsileaceae / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D000431:Ethanol; D008315:Malondialdehyde; D011794:Quercetin
keywords: DPPH; Marsilea quadrifolia; ORAC; antioxidant activities; polyphenol; restraint stress