title: Magnetically separable polymer (Mag-MIP) for selective analysis of biotin in food samples.
abstract: This work presents an efficient method for the preparation of magnetic nanoparticles modified with molecularly imprinted polymers (Mag-MIP) through core-shell method for the determination of biotin in milk food samples. The functional monomer acrylic acid was selected from molecular modeling, EGDMA was used as cross-linking monomer and AIBN as radical initiator. The Mag-MIP and Mag-NIP were characterized by FTIR, magnetic hysteresis, XRD, SEM and N2-sorption measurements. The capacity of Mag-MIP for biotin adsorption, its kinetics and selectivity were studied in detail. The adsorption data was well described by Freundlich isotherm model with adsorption equilibrium constant (KF) of 1.46 mL g(-1). The selectivity experiments revealed that prepared Mag-MIP had higher selectivity toward biotin compared to other molecules with different chemical structure. The material was successfully applied for the determination of biotin in diverse milk samples using HPLC for quantification of the analyte, obtaining the mean value of 87.4% recovery.
authors.affiliation: UNESP - Univ Estadual Paulista, Institute of Chemistry, Department of Analytical Chemistry, 14801-970 Araraquara, SP, Brazil; Laboratory of Physical Chemistry Research, Faculty of Science, National University of Engineering, Tupac Amaru 210 Av., Rimac, Lima, Peru.
authors.forename: Rosario Josefina
authors.initials: RJ
authors.lastname: Uzuriaga-Sánchez
authors.affiliation: UNESP - Univ Estadual Paulista, Institute of Chemistry, Department of Analytical Chemistry, 14801-970 Araraquara, SP, Brazil.
authors.forename: Sabir
authors.initials: S
authors.lastname: Khan
authors.affiliation: UNESP - Univ Estadual Paulista, Institute of Chemistry, Department of Analytical Chemistry, 14801-970 Araraquara, SP, Brazil.
authors.forename: Ademar
authors.initials: A
authors.lastname: Wong
authors.affiliation: Laboratory of Physical Chemistry Research, Faculty of Science, National University of Engineering, Tupac Amaru 210 Av., Rimac, Lima, Peru.
authors.forename: Gino
authors.initials: G
authors.lastname: Picasso
authors.affiliation: Group de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Pividori
authors.affiliation: UNESP - Univ Estadual Paulista, Institute of Chemistry, Department of Analytical Chemistry, 14801-970 Araraquara, SP, Brazil. Electronic address: mpilar@iq.unesp.br.
authors.forename: Maria Del Pilar Taboada
authors.initials: MDPT
authors.lastname: Sotomayor
mesh_terms: D000327:Adsorption; D000818:Animals; D001710:Biotin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D064347:Microscopy, Electrochemical, Scanning; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011108:Polymers; D001710:Biotin
keywords: Biotin; Imprinted polymers; Magnetic nanoparticles; Magnetic-MIP; Milk