title: Supporting data for comparative proteomic analysis of Listeria monocytogenes ATCC 7644 exposed to a sublethal concentration of nisin.
abstract: Here we provide the LC-MS/MS data from a comparative analysis of Listeria monocytogenes ATCC 7644 treated and non-treated with a sublethal concentration of nisin (10(-3) mg/mL). Protein samples were analyzed by multidimensional protein identification technology (MudPIT) approach, in an off-line configuration. The raw MS/MS data allowed the detection of 49,591 spectra which resulted in 576 protein identifications. After Scaffold validation, 179 proteins were identified with high confidence. A label-free quantitative analysis based of normalized spectral abundance factor (NSAF) was used and 13 proteins were found differentially expressed between nisin-treated and non-treated cells. Gene ontology analysis of differentially expressed proteins revealed that most of them are correlated to metabolic process, oxidative stress response mechanisms and molecular binding. A detailed analysis and discussion of these data may be found in Miyamoto et al. [1].
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Kendi Nishino
authors.initials: KN
authors.lastname: Miyamoto
authors.affiliation: Laboratório de Genômica Estrutura e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, Brazil ; Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Mariante Monteiro
authors.affiliation: Laboratório de Genômica Estrutura e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, Brazil.
authors.forename: Karin
authors.initials: K
authors.lastname: da Silva Caumo
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil ; Laboratório de Genômica Estrutura e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Rodrigues Lorenzatto
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil ; Laboratório de Genômica Estrutura e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, Brazil ; Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, Brazil.
authors.forename: Henrique
authors.initials: H
authors.lastname: Bunselmeyer Ferreira
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil ; Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, UFRGS, Porto Alegre, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli