title: Structural and functional changes in high-density lipoprotein induced by chemical modification.
abstract: Reconstituted high-density lipoprotein (rHDL), a natural nanoparticle consisting of apolipoprotein A-I and phospholipids, was modified with a hydrophobic fluorescent dye before (pre-rHDL) and after (post-rHDL) reconstitution. Pre-rHDL particles had a similar size to unmodified rHDL, but post-rHDL particles were significantly larger and their avidity for a HDL receptor was 2.6 times of that shown by pre-rHDL.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. murakami@icems.kyoto-u.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murakami
authors.forename: Haruki
authors.initials: H
authors.lastname: Okamoto
authors.forename: Hyungjin
authors.initials: H
authors.lastname: Kim
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000378:metabolism; D015243:Cholesterol, VLDL / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry
chemical_list: D016632:Apolipoprotein A-I; D015243:Cholesterol, VLDL; D008075:Lipoproteins, HDL; D008823:Micelles; D010743:Phospholipids