title: Capillary electrophoresis of sialylated oligosaccharides in milk from different species.
abstract: Oligosaccharides are relevant components of human milk, which have been quite well studied for their pre-biotic effect and their capacity in stimulating the immune system. Since oligosaccharides from milk of non-human mammals received so far less attention, the aim of this work was the application of capillary electrophoresis (CE) for the analysis of sialylated oligosaccharides in cow, goat and equine (mare and donkey) milk to possibly identify potential sources of oligosaccharides to use as health promoting ingredients in functional foods. Human milk was used as reference milk. A recent CE technique was applied to resolve and quantify 3-sialyllactose (3-SL), 6-sialyllactose (6-SL) and disialyl-lacto-N-tetraose (DSLNT). Analysis of non-human milk samples confirmed differences among species and individuals: DSLNT, which was the most abundant compound in human milk (455-805μg/mL) was missing in most of the samples. In most cases, 3-SL showed to be the most concentrated of the quantified analytes, with values ranging from 12 to 77μg/mL.
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria - Centro di ricerca per le produzioni foraggere e lattiero-casearie (CRA-FLC), Via A. Lombardo 11, 26900 Lodi, Italy. Electronic address: lucia.monti@entecra.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Monti
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria - Unità di ricerca per i processi dell'industria agroalimentare (CRA-IAA), Via Venezian, 26, 20133 Milano, Italy.
authors.forename: Tiziana Maria Piera
authors.initials: TM
authors.lastname: Cattaneo
authors.affiliation: Dipartimento di Scienze Veterinarie, Università di Pisa, V.le delle Piagge 2, 56124 Pisa, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Orlandi
authors.affiliation: Dipartimento di Scienze Veterinarie, Università di Pisa, V.le delle Piagge 2, 56124 Pisa, Italy.
authors.forename: Maria Claudia
authors.initials: MC
authors.lastname: Curadi
mesh_terms: D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary; D018397:Equidae; D005260:Female; D006801:Humans; D007785:Lactose / Q000031:analogs & derivatives / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis*; D013045:Species Specificity
chemical_list: C421467:3'-sialyllactose; C403777:6'-sialyllactose; D009844:Oligosaccharides; C576246:disialyllacto-N-tetraose; D007785:Lactose
keywords: Capillary electrophoresis; Mammalian milk; Sialylated oligosaccharides