title: Duration of priapism is associated with increased corporal oxidative stress and antioxidant enzymes in a rat model.
abstract: Ischaemic priapism is characterised by hypoxia, hypercapnia and acidosis with resultant corporal fibrosis. Studies reported decreased erectile recovery after treatment of priapism longer than 36 h. However, a recent study revealed that half of patients with 3 days of priapism achieved recovery after T-shunt, although mechanism remains unclear. We aimed to investigate the effect of priapism duration on oxidative stress and antioxidant enzymes. Twenty-four male rats were divided into four groups. Group 1 served as control. Groups 2, 3 and 4 represented 1, 2 and 4 h, respectively, of priapism induced by vacuum device and rubber band placed at base of erect penis. After 30 min of reperfusion, penectomy and blood withdrawal were performed to investigate levels of malondialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx). Corporal MDA progressively increased with priapism duration (P = 0.01). Corporal SOD significantly differed between groups 1, 2 and 4. Also, there were significant differences in corporal GPx in groups 1 and 4 (P = 0.004) and groups 2 and 4 (P = 0.01). Corporal CAT was higher in group 4, but multivariable analysis revealed insignificant differences. Plasma MDA of the experimental groups was significantly higher than that of controls. There were no differences among groups in terms of other parameters. Increased antioxidant enzymes according to duration of priapism suggest that immediate treatment to relieve oxidative stress should be initiated in prolonged cases. However, further studies should be conducted to determine resistance mechanisms of the corpora to prolonged ischaemia.
authors.affiliation: Istanbul University, Istanbul Medical Faculty, Department of Urology, Istanbul, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Kucukdurmaz
authors.affiliation: Istanbul University Istanbul Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Kucukgergin
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Urology, Istanbul, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Akman
authors.affiliation: Istanbul University, Istanbul Medical Faculty, Department of Urology, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Salabas
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Urology, Istanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Armagan
authors.affiliation: Istanbul University Istanbul Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Seckin
authors.affiliation: Istanbul University, Istanbul Medical Faculty, Department of Urology, Istanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Kadıoglu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000032:analysis; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000032:analysis; D006801:Humans; D007511:Ischemia / Q000150:complications*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress; D010413:Penis / Q000378:metabolism*; D011317:Priapism / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidants; erectile dysfunction; oxidative stress; priapism; shunt