title: Two-year comparison of the biochemical properties of polyphenol oxidase from Turkish Alyanak apricot (Prunus armenica L.).
abstract: Polyphenol oxidase (PPO) was extracted and purified from Turkish Alyanak apricot variety and its characteristics were studied in two consecutive years (2008 and 2009). Three isoenzymes (isoenzyme A1, A2 and B) were obtained upon ammonium sulfate fractionation, ion exchange chromatography using DEAE-Toyopearl 650 M and gel filtration chromatography using Sephadex G-100. The isoenzymes exhibited different kinetic properties. Furthermore, year-to-year variability in Km and Vmax values was significant. The pH optimum for enzyme activity was 4.98 for isoenzymes A1 and A2, and 5.8 for isoenzyme B. The isoenzymes A1 and B had optimum temperature at 30 °C in both years whereas isoenzyme A2 had maximum activity at 40 °C in 2008 and 30 °C in 2009. The inactivation kinetics parameters and the effects of inhibitors tested exhibited significant year-to-year variation.
authors.affiliation: University of Cukurova, Faculty of Agriculture, Department of Food Engineering, Balcali, 01330 Adana, Turkey. Electronic address: muunal@cu.edu.tr.
authors.forename: M Ümit
authors.initials: MÜ
authors.lastname: Ünal
authors.affiliation: University of Adiyaman, Faculty of Engineering, Department of Food Engineering, Altinsehir, 02040 Adiyaman, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Şener
mesh_terms: D004156:Catechol Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000068219:Prunus armeniaca / Q000737:chemistry*; D014421:Turkey
chemical_list: D004156:Catechol Oxidase
keywords: Alyanak apricot; Harvest year; Kinetics; Polyphenol oxidase; Thermal inactivation