title: Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress.
abstract: We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25 °C) and in liquid crystalline states (50 °C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50 °C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25 °C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects.
authors.affiliation: Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales, Centro de Investigaciones y Transferencia Santiago del Estero, Universidad Nacional de Santiago del Estero, CP 4206 Santiago del Estero, Argentina.
authors.forename: A C
authors.initials: AC
authors.lastname: Cutró
authors.affiliation: Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales, Centro de Investigaciones y Transferencia Santiago del Estero, Universidad Nacional de Santiago del Estero, CP 4206 Santiago del Estero, Argentina.
authors.forename: E A
authors.initials: EA
authors.lastname: Disalvo
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D015394:Molecular Structure; D009997:Osmotic Pressure; D010649:Phenylalanine / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D005782:Gels; D008563:Membrane Lipids; D053835:Unilamellar Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D010649:Phenylalanine