title: Two-photon microscopy of a Flt1 peptide-hyaluronate conjugate.
abstract: OBJECTIVE
  Two-photon microscopy was performed to visualize ocular distribution of Flt1 peptide-hyaluronate (HA) conjugate micelles for eye drop treatment of corneal neovascularization.
  
  
  METHODS
  Flt1 peptide-HA conjugate micelles were topically administered to the eye for two-photon microscopy and antiangiogenic effect assessment after silver nitrate cauterization.
  
  
  RESULTS
  In vivo two-photon microscopy revealed that Flt1 peptide-HA conjugate micelles were absorbed and remained on the corneal epithelia with an increased residence time, facilitating the corneal delivery of carboxyfluorescein succinimidyl ester (CFSE) as a model drug. Furthermore, repeated eye drops of Flt1 peptide-HA conjugate micelles showed comparable therapeutic effect to the subconjunctival injection on the corneal neovascularization.
  
  
  CONCLUSIONS
  We confirmed the feasibility of Flt1 peptide-HA conjugate micelles for eye drop treatment of corneal neovascularization.
authors.affiliation: Department of Materials Science & Engineering, Pohang University of Science & Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Korea.
authors.forename: Songeun
authors.initials: S
authors.lastname: Beack
authors.affiliation: Department of Ophthalmology & Visual Science, Seoul St., Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Korea.
authors.forename: Jun-Sub
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Division of Biosciences & Biotechnology, Pohang University of Science & Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Materials Science & Engineering, Pohang University of Science & Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Biosciences & Biotechnology, Pohang University of Science & Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Korea.
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology & Visual Science, Seoul St., Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Korea.
authors.forename: Choun-Ki
authors.initials: CK
authors.lastname: Joo
authors.affiliation: Department of Materials Science & Engineering, Pohang University of Science & Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Korea.
authors.forename: Sei Kwang
authors.initials: SK
authors.lastname: Hahn
mesh_terms: D000818:Animals; D003315:Cornea / Q000098:blood supply*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000627:therapeutic use; D008823:Micelles; D008853:Microscopy / Q000379:methods*; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010455:Peptides / Q000737:chemistry*; D017785:Photons; D051381:Rats; D017207:Rats, Sprague-Dawley; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D008823:Micelles; D010455:Peptides; D006820:Hyaluronic Acid; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: Flt1 peptide; corneal neovascularization; eye drop; hyaluronate; two-photon microscopy