title: OsPIN5b modulates rice (Oryza sativa) plant architecture and yield by changing auxin homeostasis, transport and distribution.
abstract: Plant architecture attributes such as tillering, plant height and panicle size are important agronomic traits that determine rice (Oryza sativa) productivity. Here, we report that altered auxin content, transport and distribution affect these traits, and hence rice yield. Overexpression of the auxin efflux carrier-like gene OsPIN5b causes pleiotropic effects, mainly reducing plant height, leaf and tiller number, shoot and root biomass, seed-setting rate, panicle length and yield parameters. Conversely, reduced expression of OsPIN5b results in higher tiller number, more vigorous root system, longer panicles and increased yield. We show that OsPIN5b is an endoplasmic reticulum (ER) -localized protein that participates in auxin homeostasis, transport and distribution in vivo. This work describes an example of an auxin-related gene where modulating its expression can simultaneously improve plant architecture and yield potential in rice, and reveals an important effect of hormonal signaling on these traits.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Lu
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Coneva
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: José A
authors.initials: JA
authors.lastname: Casaretto
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Shan
authors.initials: S
authors.lastname: Ying
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Kashif
authors.initials: K
authors.lastname: Mahmood
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3B2, Canada.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nambara
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Bi
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Rothstein
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D001692:Biological Transport; D018533:Biomass; D018556:Crops, Agricultural / Q000254:growth & development / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D012275:Oryza / Q000033:anatomy & histology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins
keywords: Oryza sativa; PIN; auxin; plant growth; rice; yield