title: Intermittent fasting modulates IgA levels in the small intestine under intense stress: a mouse model.
abstract: Intermittent fasting prolongs the lifespan and unlike intense stress provides health benefits. Given the role of the immunoglobulin A (IgA) in the intestinal homeostasis, the aim of this study was to assess the impact of intermittent fasting plus intense stress on secretory IgA (SIgA) production and other mucosal parameters in the duodenum and ileum. Two groups of six mice, with intermittent fasting or fed ad libitum for 12weeks, were submitted to a session of intense stress by a bout of forced swimming. Unstressed ad libitum fed or intermittently fasted groups were included as controls. After sacrifice, we evaluated intestinal SIgA and plasma adrenal hormones, lamina propria IgA+ plasma-cells, mRNA expression of polymeric immunoglobulin receptor, α- and J-chains in the liver and intestinal mucosa, as well as pro- (tumor necrosis factor-α, interleukin-6 and Interferon-γ) and anti- (interleukin-2, -4, -10 and transforming growth factor-β) inflammatory cytokines in mucosal samples. Under intense stress, intermittent fasting down- or up-modulated the levels of most parameters in the duodenum and ileum, respectively while up-regulated corticosterone levels without affecting epinephrine. Our data suggest intermittent fasting plus intense stress elicited neuroendocrine pathways that differentially controlled IgA and pIgR expression in duodenum and ileum. These findings provide experimental foundations for a presumable impact of intermittent fasting under intense stress on the intestinal homeostasis or inflammation by triggering or reducing the IgA production in ileum or duodenum respectively.
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico.
authors.forename: Eleazar
authors.initials: E
authors.lastname: Lara-Padilla
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico.
authors.forename: Marycarmen
authors.initials: M
authors.lastname: Godínez-Victoria
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana, Xochimilco, Calzada del Hueso No 1100, Col. Villa Quietud, CP 04960 México, D.F., Mexico.
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Drago-Serrano
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico.
authors.forename: Humberto
authors.initials: H
authors.lastname: Reyna-Garfias
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico.
authors.forename: Ivonne Maciel
authors.initials: IM
authors.lastname: Arciniega-Martínez
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Abarca-Rojano
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico.
authors.forename: Teresita Rocío
authors.initials: TR
authors.lastname: Cruz-Hernández
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, CP 11340 México, D.F., Mexico. Electronic address: citlicampos@gmail.com.
authors.forename: Rafael
authors.initials: R
authors.lastname: Campos-Rodríguez
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004386:Duodenum / Q000378:metabolism*; D005215:Fasting / Q000378:metabolism* / Q000523:psychology; D007082:Ileum / Q000378:metabolism*; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013315:Stress, Psychological / Q000378:metabolism* / Q000523:psychology; D013997:Time Factors
chemical_list: D007070:Immunoglobulin A
keywords: Diet; Intestinal homeostasis; Secretory IgA; Stress hormones; pIgR