title: Mixed poly(dopamine)/poly(L-lysine) (composite) coatings: from assembly to interaction with endothelial cells.
abstract: Engineered polymer films are of significant importance in the field of biomedicine. Poly(dopamine) (PDA) is becoming more and more a key player in this context. Herein, we deposited mixed films consisting of PDA and poly(L-lysine) (PLL) of different molecular weights. The coatings were characterized by quartz crystal microbalance with dissipation monitoring, atomic force microscopy, and X-ray photoelectron spectroscopy. The protein adsorption to the mixed films was found to decrease with increasing amounts of PLL. PDA/PLL capsules were also successfully assembled. Higher PLL content in the membranes reduced their thickness while the ζ-potential increased. Further, endothelial cell adhesion and proliferation over 96 h were found to be independent of the type of coating. Using PDA/PLL in liposome-containing composite coatings showed that sequential deposition of the layers yielded higher liposome trapping compared to one-step adsorption except for negatively charged liposomes. Association/uptake of fluorescent cargo by adherent endothelial cells was found to be different for PDA and PDA/PLL films. Taken together, our findings illustrate the potential of PDA/PLL mixed films as coatings for biomedical applications.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark. bstadler@inano.au.dk.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Martin E
authors.initials: ME
authors.lastname: Lynge
authors.forename: Boon M
authors.initials: BM
authors.lastname: Teo
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ogaki
authors.forename: Brigitte
authors.initials: B
authors.lastname: Städler
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D042783:Endothelial Cells / Q000737:chemistry* / Q000187:drug effects; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D007211:Indoles; D008081:Liposomes; D011108:Polymers; C568283:polydopamine; D008239:Lysine