title: BTEX biodegradation and its nitrogen removal potential by a newly isolated Pseudomonas thivervalensis MAH1.
abstract: Benzene, toluene, ethylbenzene, and xylene (BTEX) are of great environmental concern because of their widespread occurrence in groundwater and soil, posing an increasing threat to human health. The aerobic denitrifying BTEX-degrading bacterium Pseudomonas thivervalensis MAH1 was isolated from BTEX-contaminated sediment under nitrate-reducing conditions. The degradation rates of benzene, toluene, ethylbenzene, and xylene by strain MAH1 were 4.71, 6.59, 5.64, and 2.59 mg·L⁻¹day⁻¹, respectively. The effects of sodium citrate, nitrate, and NaH2PO4 on improving BTEX biodegradation were investigated, and their optimum concentrations were 0.5 g·L⁻¹, 100 mg·L⁻¹, and 0.8 mmol·L⁻¹, respectively. Moreover, MAH1, which has nirS and nosZ genes, removed ammonium, nitrate, and nitrite at 2.49 mg NH(4)(+)-N·L⁻¹·h⁻¹, 1.50 mg NO(3)(-)-N·L⁻¹·h⁻¹, and 0.83 mg NO(2)(-)-N·L⁻¹·h⁻¹, respectively. MAH1 could help in mitigating the pollution caused by nitrogen amendments for biostimulation. This study highlighted the feasibility of using MAH1 for the bioremediation of BTEX-contaminated sites.
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.|Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Qu
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.|Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.|Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.|Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.
authors.forename: Hejun
authors.initials: H
authors.lastname: Ren
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.|Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun 130021, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
mesh_terms: D001554:Benzene / Q000378:metabolism*; D001555:Benzene Derivatives / Q000378:metabolism*; D001673:Biodegradation, Environmental; D058440:Denitrification; D060587:Groundwater / Q000737:chemistry; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D014050:Toluene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism*
chemical_list: D001555:Benzene Derivatives; D009566:Nitrates; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D014992:Xylenes; D014050:Toluene; D001554:Benzene; C004912:ethylbenzene; D009584:Nitrogen
keywords: BTEX; Pseudomonas thivervalensis MAH1; biodegradation; biodégradation; bioremediation; biorestauration; nitrogen removal; élimination de l’azote