title: [The function and the significance of full-automated tests for detecting antiphospholipid antibodies].
abstract: Antiphospholipid antibodies (aPLs) are a group of heterogenous antibodies with immunological and functional variations that are detected in the sera of patients with antiphospholipid syndrome (APS). Detection of these antibodies in an efficient and accurate manner remains a significant issue. It requires numerous immunological and functional tests, burdening the laboratory departments, and as a consequence, not sufficiently performed in many cases. We retrospectively studied a total of 212 subjects with or without collagen diseases including APS that visited the outpatients of multiple institutions (department of internal medicine at Health Science University of Hokkaido, department of medicine II and department of gastroenterology at Hokkaido University Hospital). All the subjects were measured aPL (anticardio anticardiolipin antibody IgG/IgM, anti-β2-glycoprotein I antibody IgG/IgM) using a fully automated chemiluminescence analyzer and compared measurement results with those obtained using the conventional ELISA method. These methods were found to have similar diagnostic accuracy, with κ values exceeding 0.6. Of 61 APS patients 41 (67%) were positive for two or more tests: significantly higher than other disease such as systemic lupus erythematosus (3/37, 9%) or non-SLE collagen disease (1/53, 2%). The fully automated chemiluminescence analyzer, which can simultaneously measure multiple aPLs, was thus determined to be useful for diagnosing APS.
authors.affiliation: Department of Rheumatology, Endocrinology and Nephrology, Hokkaido University Graduate School of Medicine.
authors.forename: Kenji
authors.initials: K
authors.lastname: Oku
authors.forename: Olga
authors.initials: O
authors.lastname: Amengual
authors.forename: Ryo
authors.initials: R
authors.lastname: Hisada
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Oomura
authors.forename: Ikuma
authors.initials: I
authors.lastname: Nakagawa
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Bohgaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Horita
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Yasuda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Atsumi
mesh_terms: D017152:Antibodies, Antiphospholipid / Q000032:analysis*; D016736:Antiphospholipid Syndrome / Q000276:immunology; D001331:Automation; D002308:Cardiolipins / Q000276:immunology; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D012189:Retrospective Studies; D053482:beta 2-Glycoprotein I
chemical_list: D017152:Antibodies, Antiphospholipid; D002308:Cardiolipins; D053482:beta 2-Glycoprotein I