title: IL-10 producing B cells partially restore E2-mediated protection against EAE in PD-L1 deficient mice.
abstract: Women with multiple sclerosis (MS) often experience clinical improvement during pregnancy, indicating that sex hormones might have therapeutic effects in MS. Our previous studies have demonstrated that B cells and PD-L1 are crucial for E2 (17β-estradiol)-mediated protection against experimental autoimmune encephalomyelitis (EAE). We here demonstrate that the transfer of IL-10(+) B cells into E2-treated PD-L1(-/-) mice after EAE induction could partially restore E2-mediated protection and decrease the frequency of pro-inflammatory cells in the CNS compared to E2/saline treated PD-L1(-/-) mice. Hence, co-administration of IL-10(+) B cells and E2 might have a powerful therapeutic potential for treatment of EAE.
authors.affiliation: Neuroimmunology Research, VA Portland Health Care System, Portland, OR, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, USA. Electronic address: zhanju@ohsu.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Neuroimmunology Research, VA Portland Health Care System, Portland, OR, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, USA. Electronic address: benedek@ohsu.edu.
authors.forename: Gil
authors.initials: G
authors.lastname: Benedek
authors.affiliation: Neuroimmunology Research, VA Portland Health Care System, Portland, OR, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, USA. Electronic address: bodhanka@ohsu.edu.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Bodhankar
authors.affiliation: Neuroimmunology Research, VA Portland Health Care System, Portland, OR, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, USA. Electronic address: lapato@ohsu.edu.
authors.forename: Andrew
authors.initials: A
authors.lastname: Lapato
authors.affiliation: Neuroimmunology Research, VA Portland Health Care System, Portland, OR, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, USA; Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR, USA. Electronic address: vandenba@ohsu.edu.
authors.forename: Arthur A
authors.initials: AA
authors.lastname: Vandenbark
authors.affiliation: Neuroimmunology Research, VA Portland Health Care System, Portland, OR, USA; Department of Neurology, Oregon Health & Science University, Portland, OR, USA; Department of Anesthesiology and Perioperative Medicine, Oregon Health & Science University, Portland, OR, USA. Electronic address: offnerva@ohsu.edu.
authors.forename: Halina
authors.initials: H
authors.lastname: Offner
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000637:transplantation; D060890:B7-H1 Antigen / Q000172:deficiency* / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism* / Q000517:prevention & control*; D004958:Estradiol / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; C556062:IL10 protein, mouse; D018696:Neuroprotective Agents; D016753:Interleukin-10; D004958:Estradiol
keywords: EAE; Estrogen; IL-10; PD-L1; PD-L2; Regulatory B cell