title: Syntaxin 1B is important for mouse postnatal survival and proper synaptic function at the mouse neuromuscular junctions.
abstract: STX1 is a major neuronal syntaxin protein located at the plasma membrane of the neuronal tissues. Rodent STX1 has two highly similar paralogs, STX1A and STX1B, that are thought to be functionally redundant. Interestingly, some studies have shown that the distribution patterns of STX1A and STX1B at the central and peripheral nervous systems only partially overlapped, implying that there might be differential functions between these paralogs. In the current study, we generated an STX1B knockout (KO) mouse line and studied the impact of STX1B removal in neurons of several brain regions and the neuromuscular junction (NMJ). We found that either complete removal of STX1B or selective removal of it from forebrain excitatory neurons in mice caused premature death. Autaptic hippocampal and striatal cultures derived from STX1B KO mice still maintained efficient neurotransmission compared with neurons from STX1B wild-type and heterozygous mice. Interestingly, examining high-density cerebellar cultures revealed a decrease in the spontaneous GABAergic transmission frequency, which was most likely due to a lower number of neurons in the STX1B KO cultures, suggesting that STX1B is essential for neuronal survival in vitro. Moreover, our study also demonstrated that although STX1B is dispensable for the formation of the mouse NMJ, it is required to maintain the efficiency of neurotransmission at the nerve-muscle synapse.
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany;
authors.forename: Yuan-Ju
authors.initials: YJ
authors.lastname: Wu
authors.affiliation: Department of Medical Physiology and Biophysics, School of Medicine, University of Seville, Seville, Spain; and.
authors.forename: Rocio
authors.initials: R
authors.lastname: Tejero
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany;
authors.forename: Marife
authors.initials: M
authors.lastname: Arancillo
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany;
authors.forename: Gülcin
authors.initials: G
authors.lastname: Vardar
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany; Leibniz Institute for Molecular Pharmacology, Berlin, Germany.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Korotkova
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany;
authors.forename: Michael
authors.initials: M
authors.lastname: Kintscher
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany;
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schmitz
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany; Leibniz Institute for Molecular Pharmacology, Berlin, Germany.
authors.forename: Alexey
authors.initials: A
authors.lastname: Ponomarenko
authors.affiliation: Department of Medical Physiology and Biophysics, School of Medicine, University of Seville, Seville, Spain; and.
authors.forename: Lucia
authors.initials: L
authors.lastname: Tabares
authors.affiliation: NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Berlin, Germany; christian.rosenmund@charite.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Rosenmund
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000473:pathology / Q000503:physiopathology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003643:Death; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D007150:Immunohistochemistry; D053444:Inhibitory Postsynaptic Potentials / Q000502:physiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D055369:Miniature Postsynaptic Potentials / Q000502:physiology; D051938:Munc18 Proteins / Q000378:metabolism; D009469:Neuromuscular Junction / Q000502:physiology*; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D018408:Patch-Clamp Techniques; D050827:Syntaxin 1 / Q000235:genetics / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D051938:Munc18 Proteins; C497938:Stxbp1 protein, mouse; D050827:Syntaxin 1; C000608868:syntaxin 1B, mouse; D005680:gamma-Aminobutyric Acid
keywords: NMJ; SNARE; evoked release; neurotransmitter release; spontaneous release