title: Gold Nanoparticles Promote Oxidant-Mediated Activation of NF-κB and 53BP1 Recruitment-Based Adaptive Response in Human Astrocytes.
abstract: Nanogold-based materials are promising candidate tools for nanobased medicine. Nevertheless, no conclusive information on their cytotoxicity is available. In the present study, we investigated the effects of gold nanoparticles (AuNPs) on human astrocytes in vitro. Nanogold treatment in a wide range of concentrations did not result in cytotoxicity. In contrast, nanogold provoked changes in the astrocyte cell cycle and induced senescence-associated β-galactosidase activity. AuNPs promoted oxidative stress and caused activation of NF-κB pathway. After nanogold treatment, an inverse correlation between the formation of 53BP1 foci and micronuclei generation was observed. The robust 53BP1 recruitment resulted in reduced micronuclei production. Thus, nanogold treatment stimulated an adaptive response in a human astrocyte cell.
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mytych
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.affiliation: Department of Plant Physiology, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Zebrowski
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D006046:Gold / Q000009:adverse effects* / Q000627:therapeutic use; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D053768:Metal Nanoparticles / Q000009:adverse effects* / Q000627:therapeutic use; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics*; D050997:Nanomedicine; D000071857:Tumor Suppressor p53-Binding Protein 1
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D016328:NF-kappa B; C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1; D006046:Gold