title: Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos.
abstract: Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both polyadenylated and non-polyadenylated RNAs from individual cells. This method exhibits robust sensitivity, precision and accuracy. We discover 2891 circRNAs and 913 novel linear transcripts in mouse preimplantation embryos and further analyze the abundance of circRNAs along development, the function of enriched genes, and sequence features of circRNAs. Our work is key to deciphering regulation mechanisms of circRNAs during mammalian early embryonic development.
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. sharryfan100@gmail.com.|College of Life Sciences, Peking University, Beijing, 100871, China. sharryfan100@gmail.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Fan
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. friedpine@gmail.com.|College of Engineering, Peking University, Beijing, 100871, China. friedpine@gmail.com.
authors.forename: Xiannian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. wuxinglong11@sina.com.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. wuxinglong11@sina.com.|Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China. wuxinglong11@sina.com.
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. guohongshan1024@gmail.com.|College of Life Sciences, Peking University, Beijing, 100871, China. guohongshan1024@gmail.com.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. yqhu2012@126.com.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. yqhu2012@126.com.|Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China. yqhu2012@126.com.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. tangfuchou@pku.edu.cn.|College of Life Sciences, Peking University, Beijing, 100871, China. tangfuchou@pku.edu.cn.|Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Peking University, Beijing, 100871, China. tangfuchou@pku.edu.cn.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. tangfuchou@pku.edu.cn.|Center for Molecular and Translational Medicine, Peking University Health Science Center, Beijing, 100191, China. tangfuchou@pku.edu.cn.
authors.forename: Fuchou
authors.initials: F
authors.lastname: Tang
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China. yanyi@pku.edu.cn.|College of Engineering, Peking University, Beijing, 100871, China. yanyi@pku.edu.cn.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. yanyi@pku.edu.cn.
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D000079962:RNA, Circular; D017423:Sequence Analysis, RNA / Q000379:methods*; D059010:Single-Cell Analysis; D059467:Transcriptome
chemical_list: D000079962:RNA, Circular; D012313:RNA