title: The iPLA(2)γ is identified as the membrane potential sensitive phospholipase in liver mitochondria.
abstract: Previous reports from our lab identified a mitochondrial calcium-independent phospholipase A2 activity that is activated when the mitochondrial membrane potential is decreased. This activity was demonstrated to influence occurrence of the permeability transition. Originally, this activity was ascribed to the iPLA2β protein. Recently, both iPLA2β and iPLA2γ knock out mice have been generated. It has been shown by others that the iPLA2γ plays a significant role in progression of the permeability transition. In this paper, using the iPLA2β and iPLA2γ knock out mice we show that the membrane potential sensitive activity is the iPLA2γ.
authors.affiliation: Department of Cellular and Molecular Biochemistry, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Rauckhorst
authors.affiliation: Department of Cellular and Molecular Biochemistry, The Ohio State University, Columbus, OH 43210, United States; Internal Medicine, The Ohio State University, Columbus, OH 43210, United States; Institute of Mitochondrial Biology, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Pfeiffer
authors.affiliation: Department of Chemistry and Biochemistry, Ohio Northern University, Ada, OH 45810, United States. Electronic address: k-broekemeier@onu.edu.
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Broekemeier
mesh_terms: D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D055786:Gene Knockout Techniques; D054522:Group VI Phospholipases A2 / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008930:Mitochondria, Liver / Q000378:metabolism*; D009281:Naphthalenes / Q000494:pharmacology; D011753:Pyrones / Q000494:pharmacology; D051381:Rats
chemical_list: D009281:Naphthalenes; D011753:Pyrones; C068314:6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one; D054522:Group VI Phospholipases A2; C516476:Pla2g6 protein, mouse
keywords: Bioenergetics; Calcium-independent phospholipase A2; Knockout; Mitochondria; Phospholipase