title: Aroma volatiles obtained at harvest by HS-SPME/GC-MS and INDEX/MS-E-nose fingerprint discriminate climacteric behaviour in melon fruit.
abstract: BACKGROUND
  Melon aroma volatiles were extracted at harvest from juice of a climacteric near-isogenic line (NIL) SC3-5-1 with two quantitative trait loci (QTLs) introgressed which produced climacteric behaviour and its non-climacteric parental (PS) using two methodologies of analysis: static headspace solid phase micro-extraction (HS-SPME) by gas chromatography-mass spectrometry (GC-MS) and inside needle dynamic extraction (INDEX) by MS-based electronic nose (MS-E-nose).
  
  
  RESULTS
  Of the 137 volatiles compounds identified, most were found at significantly higher concentrations in SC3-5-1 than in PS in both seasons. These volatiles were mostly esters, alcohols, sulfur-derived esters and even some aldehydes and others. The number of variables with high correlation values was reduced by using correlation network analysis. Partial least squares-discriminant analysis (PLS-DA) achieved the correct classification of PS and SC3-5-1. The ions m/z 74, 91, 104, 105, 106 and 108, mainly volatile derivatives precursor phenylalanine, were the most discriminant in SC3-5-1 and PS. As many as 104 QTLs were mapped in season 1 and at least 78 QTLs in each season with an effect above the PS mean.
  
  
  CONCLUSIONS
  GC-MS gave better discrimination than E-nose. Most of the QTLs that mapped in both seasons enhanced aroma volatiles associated with climacteric behaviour.
authors.affiliation: Department of Agricultural & Food Engineering, Regional Campus of International Excellence 'Campus Mare Nostrum' (CMN), Technical University of Cartagena (UPCT), Paseo Alfonso XIII, 48, ETSIA, E-30203, Cartagena, Murcia, Spain.|Institute of Plant Biotechnology, CMN, UPCT, Campus Muralla del Mar, Plaza del Hospital s/n, E-30202, Cartagena, Murcia, Spain.
authors.forename: Libia A
authors.initials: LA
authors.lastname: Chaparro-Torres
authors.affiliation: Department of Applied Mathematics and Statistics, CMN, UPCT, Doctor Fleming s/n, ETSII, E-30202, Cartagena, Murcia, Spain.
authors.forename: María C
authors.initials: MC
authors.lastname: Bueso
authors.affiliation: Department of Agricultural & Food Engineering, Regional Campus of International Excellence 'Campus Mare Nostrum' (CMN), Technical University of Cartagena (UPCT), Paseo Alfonso XIII, 48, ETSIA, E-30203, Cartagena, Murcia, Spain.|Institute of Plant Biotechnology, CMN, UPCT, Campus Muralla del Mar, Plaza del Hospital s/n, E-30202, Cartagena, Murcia, Spain.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Fernández-Trujillo
mesh_terms: D019662:Cucurbitaceae / Q000737:chemistry* / Q000235:genetics; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D055549:Volatile Organic Compounds / Q000737:chemistry*
chemical_list: D055549:Volatile Organic Compounds
keywords: Cucumis melo L; aroma extraction; aroma fingerprint; multivariate statistical techniques; near-isogenic line; quantitative trait loci