title: Effects of isoflurane preconditioning in the delayed phase on myocardial tumor necrosis factor alpha levels and caspase-3 protein expression in a rabbit model of ischemia-reperfusion injury.
abstract: This study aimed to investigate the protective effects and the mechanisms underlying these effects of isoflurane preconditioning in the delayed phase of myocardial ischemia-reperfusion injury. We randomly divided 30 healthy male New Zealand white rabbits into three groups with 10 rabbits in each group as follows: sham operation group (C group), ischemia-reperfusion group (I/R group), and 2.0% isoflurane preconditioning group (S group). Rabbits in the C group received thoracotomy for 160 min. Rabbits in the I/R group underwent left coronary artery occlusion for 40 min and reperfusion for 120 min. Rabbits in the S group received inhalation of 2.0% isoflurane and 100% oxygen for 2 h; after 24 h, rabbits in this group received the same treatment as that administered to rabbits in the I/R group. We examined the tumor necrosis factor alpha (TNF-α) levels in each group 20 min before occlusion of the left coronary, 20 and 40 min after occlusion of the left coronary artery, and 1 and 2 h after myocardial reperfusion. After reperfusion, immunoblotting was used to measure the myocardial caspase-3 expression levels, and the infarct size was measured using Evans blue and tetrazolium chloride staining. The levels of TNF-α and caspase-3 were lower in the S group than in the I/R group, and the myocardial infarct size decreased in the S group. Thus, isoflurane preconditioning in the delayed phase exerted protective effects by decreasing the myocardial caspase-3 expression and TNF-α production in a rabbit model of ischemia-reperfusion injury.
authors.affiliation: Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: K
authors.initials: K
authors.lastname: Ran
authors.affiliation: Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: D Q
authors.initials: DQ
authors.lastname: Zou
authors.affiliation: Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Xiao
authors.affiliation: Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Y T
authors.initials: YT
authors.lastname: Chang
authors.forename: K M
authors.initials: KM
authors.lastname: Duan
authors.affiliation: Department of Anesthesiology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Ou
authors.affiliation: Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China xiaoyanying_xyy@163.com.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Li
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000096:biosynthesis / Q000378:metabolism*; D019194:Ischemic Preconditioning / Q000379:methods*; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007530:Isoflurane; D053148:Caspase 3