title: Characterization of FeDREB1 promoter involved in cold- and drought-inducible expression from common buckwheat (Fagopyrum esculentum).
abstract: C-repeat-binding factor (CBF)/dehydration-responsive element (DREB) transcription factors play key roles in plant stress responses. However, little information is available on the regulation of CBF/DREB expression. In this study, we isolated and characterized the FeDREB1 promoter sequence from the common buckwheat accession Xinong 9976. To identify the upstream region of the FeDREB1 gene required for promoter activity, we constructed a series of FeDREB1 promoter deletion derivatives. Each deletion construct was analyzed through Agrobacterium-mediated transient transformation in tobacco leaves treated with 4°C cold or drought stress. Promoter-beta-glucuronidase fusion assays revealed that the pCD1 (-270 bp) deletion in the upstream region of FeDREB1 could activate expression of the GUS gene at 4°C. The pCD1 (-270 bp), pCD2 (-530 bp), and pCD3 (-904 bp) deletion induced low-level GUS expression under drought stress. However, the pCD4 (-1278 bp) deletion clearly activated GUS gene expression. Our results suggest that sections pCD1 (-270 bp) and pCD4 (-1278 bp) in the FeDREB1 gene promoter are new sources of induced promoters for adversity-resistance breeding in plant genetic engineering.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shanxi, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shanxi, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shanxi, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shanxi, China.
authors.forename: P K
authors.initials: PK
authors.lastname: Wang
authors.affiliation: Institute of Crop Genetics and Breeding/College of Agriculture, Yangtze University, Jingzhou, Hubei, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shanxi, China.
authors.forename: B L
authors.initials: BL
authors.lastname: Feng
mesh_terms: D001483:Base Sequence; D003080:Cold Temperature; D055864:Droughts; D019612:Fagopyrum / Q000235:genetics* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D005966:Glucuronidase