title: Photobiomodulation and bone healing in diabetic rats: evaluation of bone response using a tibial defect experimental model.
abstract: Diabetes mellitus (DM) leads to a delay in bone healing. Thus, some therapeutic approaches have been used to accelerate the process of bone repair such as photobiomodulation (PBM). Therefore, the present study aimed to evaluate the effects of PBM, in different fluences, in bone repair in an experimental model of tibial bone defects in diabetic rats. Sixty-four Wistar rats were submitted to a surgical procedure to perform bone defect and distributed in four groups: diabetic control group (DCG), diabetic laser group 30 J/cm(2) (L30), diabetic laser group 60 J/cm(2) (L60), and diabetic laser group 120 J/cm(2) (L120). A 808 nm Ga-Al-As (DMC Equipment, São Carlos, SP, Brazil) laser, 100 mW; 0.028 cm(2); 3.57 W/cm(2); 30, 60, and 120 J/cm(2); 0.84, 1.68, and 3.36 J; 8, 16, and 33 s was used. Animals were euthanized 15 and 30 days after the surgery. Histological, morphometric, immunohistochemistry, and biomechanical analyses were performed. In the histological and morphometric evaluation, all laser-treated groups showed a better histological pattern and a higher amount of newly formed bone compared to DCG. An intense RUNX2 immunoexpression was observed in the laser-treated groups, 15 days after the surgery. Receptor activator of nuclear factor κ-β ligand (RANK-L) immunohistochemistry analysis showed a significant decrease in the immunoreactivity for L30 and L120, 30 days after surgery. There was no statistical difference in the biomechanical analysis among the groups. In conclusion, PBM, in all fluences used, showed an osteogenic potential in bone healing of diabetic rats.
authors.affiliation: Department of Biosciences, Federal University of São Paulo, Av. Ana Costa, 95, Vila Mathias, Santos, 11060-001, São Paulo, Brazil.
authors.forename: Angela Maria Paiva
authors.initials: AM
authors.lastname: Magri
authors.forename: Kelly Rossetti
authors.initials: KR
authors.lastname: Fernandes
authors.forename: Lívia
authors.initials: L
authors.lastname: Assis
authors.forename: Nathalia Antal
authors.initials: NA
authors.lastname: Mendes
authors.forename: Ana Lúcia Yaeko
authors.initials: AL
authors.lastname: da Silva Santos
authors.forename: Edilson
authors.initials: E
authors.lastname: de Oliveira Dantas
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Rennó
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000503:physiopathology / Q000628:therapy*; D001861:Bone Regeneration; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D053685:Laser Therapy; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000473:pathology / Q000503:physiopathology*