title: Garlic and alpha lipoic supplementation enhance the immune system of albino rats and alleviate implications of pesticides mixtures.
abstract: This study aimed to investigate age dependent immune-system response versus exposure to different doses of mixture of (chlorpyrifos, profenofose, and fenitrothion) and/or combined with 60 and 250 mg kg(-1) alpha lipoic acid and garlic, respectively. 120 males of albino rats were divided to two groups according to age; weaning group (2 months age and 60-80 gm.), adult (6 months and 180-200 gm). Each age was divided into 6 subgroups treated orally for 3 months , G1 (control), G2 high dose (HDPM) CPF10 mg kg(-1), PRO 3 mg kg(-1), FEN 6 mg kg(-1), G3 low dose (LDPM) CPF 1 mg kg(-1), PFN 0.3 mg kg(-1) and FEN 0.6 mg kg(-1), G4 AOX (alpha lipoic + Garlic), G5 HDPM + AOX and G6 LDPM + AOX. Results showed significant inhibition in serum acetylcholinesterase (AChE), elevation in malondialdehyde (MDA) concurrent with reduction in total reduced glutathione (GSH) in both ages was recorded as well as, decrease in IGG, IGM, Lymphocyte transformation and Phagocytosis humeral and cellular immunity confirmed by alteration in lymph nodes architecture. This study was concluded that the supplementation with alpha lipoic acid and garlic improved previous alternations slightly to be more or less near the control level in both adult and weaning rats. It seems that, immune-responses of both adult and weaning rats were slightly similar.
authors.affiliation: Department of Biochemistry, Faculty of Science for Girls, King Abdulaziz University P.O Box 51459, Jeddah 21453, KSA.
authors.forename: Manal Ea
authors.initials: ME
authors.lastname: Elhalwagy
authors.affiliation: Department of Histology, Research Institute of Ophthalmology Giza 12211, Egypt.
authors.forename: Nevine S
authors.initials: NS
authors.lastname: Darwish
authors.affiliation: Department Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University Egypt.
authors.forename: Dina A
authors.initials: DA
authors.lastname: Shokry
authors.affiliation: Department Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University Egypt.
authors.forename: Aly Ge Abd
authors.initials: AG
authors.lastname: El-Aal
authors.affiliation: Department of Biochemistry, College of Science, King Saud University PO Box, 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Sherif H
authors.initials: SH
authors.lastname: Abd-Alrahman
authors.affiliation: Department of Mammalian Toxicology, Pesticide Central Laboratory, Agriculture Research Center Giza 12618, Egypt.
authors.forename: Abd-Alhamed
authors.initials: AA
authors.lastname: Nahas
authors.affiliation: Department of Mammalian Toxicology, Pesticide Central Laboratory, Agriculture Research Center Giza 12618, Egypt.
authors.forename: Reem M
authors.initials: RM
authors.lastname: Ziada
keywords: Organophosphorus pesticides; alpha lipoic acid; garlic; immunotoxicity; weaning