title: Bioconjugation of gold-polymer core-shell nanoparticles with bovine serum amine oxidase for biomedical applications.
abstract: Core-shell gold nanoparticles [AuNPs], stabilized with a hydrophilic polymer, poly(3-dimethylammonium-1-propyne hydrochloride) [PDMPAHCl], have been used for the immobilization of bovine serum amine oxidase [BSAO]. The functionalized surface of the hybrid nanoparticles is pH responsive, due to the presence of aminic groups that carry out a double role: on one hand they act as ligands for the gold nanoparticle surface, allowing the colloidal stabilization and, on the other hand, they give a hydrophilic characteristic to the whole colloidal suspension. The core-shell nanoparticles [Au@PDMPAHCl] have been characterized by using UV-vis and X-ray photoelectron spectroscopy, DLS, ζ-potential measurements and by FE-TEM microscopy. BSAO enzyme can be loaded by non-covalent immobilization onto Au@PDMPAHCl nanoparticles up to 70% in weight, depending on the pH values of the environmental medium. Activity tests on Au@PDMPAHCl-BSAO bioconjugates confirm an enzymatic activity up to 40%, with respect to the free enzyme activity. Moreover, our results show that loading and enzymatic activity are rather interrelated characteristics and that, under appropriate polymer concentration and pH conditions, a satisfactory compromise can be reached. These results, as a whole, indicate that Au@PDMPAHCl-BSAO bioconjugate systems are promising for future biomedical applications.
authors.affiliation: Department of Chemistry, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Venditti
authors.affiliation: Department of Chemistry, Helwan University, 11795 Helwan, Egypt.
authors.forename: T F
authors.initials: TF
authors.lastname: Hassanein
authors.affiliation: Department of Chemistry, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy. Electronic address: ilaria.fratoddi@uniroma1.it.
authors.forename: I
authors.initials: I
authors.lastname: Fratoddi
authors.affiliation: Department of Chemistry, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Fontana
authors.affiliation: Department of Physics, Roma Tre University, via della Vasca Navale 84, I-00146 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Battocchio
authors.affiliation: Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia (ITT), Viale Regina Elena 291, 00161 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Rinaldi
authors.affiliation: Dipartimento di Chimica e Tecnologia del Farmaco, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Carafa
authors.affiliation: Dipartimento di Chimica e Tecnologia del Farmaco, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Marianecci
authors.affiliation: Istituto Superiore di Sanita', Viale Regina Elena 299, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Diociaiuti
authors.affiliation: Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Agostinelli
authors.affiliation: Department of Physics, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cametti
authors.affiliation: Department of Chemistry, University of Rome "La Sapienza", P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: M V
authors.initials: MV
authors.lastname: Russo
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000818:Animals; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011108:Polymers; D006046:Gold; D006631:Amine Oxidase (Copper-Containing)
keywords: BSAO; Core–shell nanoparticles; Gold nanoparticles