title: Practical Radiation Damage-Induced Phasing.
abstract: Although crystallographers typically seek to mitigate radiation damage in macromolecular crystals, in some cases, radiation damage to specific atoms can be used to determine phases de novo. This process is called radiation damage-induced phasing or "RIP." Here, we provide a general overview of the method and a practical set of data collection and processing strategies for phasing macromolecular structures using RIP.
authors.affiliation: Structural Biology Group, European Synchrotron Radiation Facility, Grenoble, France.
authors.forename: Chloe
authors.initials: C
authors.lastname: Zubieta
authors.forename: Max H
authors.initials: MH
authors.lastname: Nanao
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D003625:Data Collection; D046911:Macromolecular Substances; D008958:Models, Molecular; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry* / Q000528:radiation effects; D011827:Radiation; D015203:Reproducibility of Results; D012984:Software; D013053:Spectrophotometry; D017356:Synchrotrons
chemical_list: D046911:Macromolecular Substances; D009696:Nucleic Acids