title: Snail promotes epithelial-mesenchymal transition and invasiveness in human ovarian cancer cells.
abstract: There are limited reports with respect to the study on the epithelium-mesenchymal transformation (EMT) mediated by Snail in the ovarian cancer. This study detected the expression of Snail and related EMT markers in the ovarian cancer tissues, and explored the possible molecular mechanism of EMT mediated by Snail in the metastasis of ovarian cancer. The patients diagnosed with ovarian cancer according to the pathology were recruited in this study during 2010-2014. The carcinoma tissue and normal tissue adjacent to carcinoma were surgically obtained from patients. The genes of E-cadherin, β-catenin, Fibronectin and N-cadherin were detected using the RT-PCR. The 64 patients were recruited and diagnosed as ovarian cancer by pathological examination. The expression levels of Snail, Fibronectin and N-cadherin in the stage III and IV were higher than those in the stage I and II, respectively (all P < 0.05). However, the expression levels of E-cadherin and β-catenin decreased along with the stage developed (trend test, both P < 0.05), respectively. The expression of Snail was positively correlated with the expression of Fibronectin, N-cadherin, but negatively correlated with the expression of E-cadherin and β-catenin. The number of A2780 cells entering into the lower compartment in the group of carcinoma tissue were significantly higher than that in the group of normal tissue after transfected with Snail expression vector. While, the invasion ability of A2780 significantly reduced after RNAi-Snail. The correlation between Snail and invasion and metastasis of ovarian cancer and epithelial-mesenchymal transition based on tissue and cell levels, and to some extent explored the molecular mechanism of the EMT process mediated by Snail.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Qiqihar Medical University Qiqihar 161042, Heilongjiang, China.
authors.forename: Yu-Lou
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Qiqihar Medical University Qiqihar 161042, Heilongjiang, China.
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Qiqihar Medical University Qiqihar 161006, P. R. China.
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Shuai
authors.affiliation: Department of Pathology, Qiqihar Medical University Qiqihar 161006, P. R. China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.affiliation: Department of Pathology, Qiqihar Medical University Qiqihar 161006, P. R. China.
authors.forename: Qing-Yang
authors.initials: QY
authors.lastname: Bai
authors.affiliation: Department of Pathology, Qiqihar Medical University Qiqihar 161006, P. R. China.
authors.forename: Xiu-Wen
authors.initials: XW
authors.lastname: Yu
authors.affiliation: Department of Pathology, Qiqihar Medical University Qiqihar 161006, P. R. China.
authors.forename: Qiu-Ting
authors.initials: QT
authors.lastname: Wen
keywords: Snail; epithelium-mesenchymal transformation; molecular mechanism; ovarian cancer