title: miR-628, a microRNA that is induced by Toll-like receptor stimulation, regulates porcine innate immune responses.
abstract: Mammalian innate and acquired immune responses involve a coordinated, sequential, and self limiting sequence of events controlled by positive and negative regulatory mechanism. MicroRNAs have been implicated as a negative regulator for diverse biological events including immune responses. However, the involvement of miRNAs in regulating the immune responses is just beginning to be explored. Here, we characterized the expression profiling of 375 microRNAs in porcine monocytes induced by lipopolysaccharide (LPS), and result shows that several of them are endotoxin-responsive genes. Through promoter analysis, the miR-628 was found to be a NF-κB dependent gene. Importantly, miR-628 was predicted to base-pair with sequences in the 3'-UTR of the myeloid differentiation protein 88 (MyD88) gene. And we found that the UTR inhibit expression of a linked reporter gene coding a key adapter molecule downstream of Toll-like receptors (TLRs), resulting in suppressing of the TLR signaling. Therefore, we not only propose a role of miR-628 in control of the TLR signaling through a negative feedback regulation loop involving down-regulation of MyD88 protein levels, but results may also contribute to rational target selection orchestrating the inflammatory responses.
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: He
authors.initials: H
authors.lastname: Jun
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: He
authors.initials: H
authors.lastname: Ying
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Daiwen
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bing
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Xiangbing
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ping
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jie
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Zhiqing
authors.affiliation: 1] Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan 625014, P. R. China [2] Key Laborotary of Animal Disease-Resistance Nutrition, Ministry of Education, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Junqiu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D017874:Immediate-Early Proteins / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics*; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics / Q000276:immunology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D017874:Immediate-Early Proteins; D008070:Lipopolysaccharides; D035683:MicroRNAs; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D051193:Toll-Like Receptors