title: Selective production of rubusoside from stevioside by using the sophorose activity of β-glucosidase from Streptomyces sp. GXT6.
abstract: In order to produce rubusoside, enzymes with preferential specificity for the saccharide sophorose were tested for ability to produce rubusoside from stevioside. We identified BGL1, a β-glucosidase from Streptomyces sp. GXT6, as an enzyme for rubusoside production. Out of several saccharide substrates, BGL1 showed the most affinity to sophorose. This enzyme only hydrolyzes the glucose moiety of the sophoroside at C-13 in stevioside. Production of rubusoside was determined by (1)H and (13)C nuclear magnetic resonance (NMR). Thus, rubusoside was produced from stevioside and the stevioside conversion rate was 98.2 %. The production yield of rubusoside was 78.8 % in 6 h.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.
authors.forename: Jinpei
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.
authors.forename: Yutuo
authors.initials: Y
authors.lastname: Wei
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi, 530007, China. haopang@gxas.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Pang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.|National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi, 530007, China.
authors.forename: Ribo
authors.initials: R
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China. duliqin@gxu.edu.cn.
authors.forename: Liqin
authors.initials: L
authors.lastname: Du
mesh_terms: D045786:Diterpenes, Kaurane / Q000096:biosynthesis* / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D005960:Glucosides / Q000096:biosynthesis* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013302:Streptomyces / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D045786:Diterpenes, Kaurane; D005936:Glucans; D005960:Glucosides; C012043:stevioside; D001617:beta-Glucosidase; C056177:rubusoside; C003328:sophorose
keywords: Rubus suavissimus; Rubusoside; Sophorose; Stevioside; Streptomyces sp; β-Glucosidase