title: Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV.
abstract: A new HPLC/UV method has been developed for the simultaneous quantitative determination of four major components in Eucommiae cortex, namely geniposidic acid (1), geniposide (2), pinoresinol di-O-β-D-glucopyranoside (3), and liriodendrin (4). Simultaneous separations of these four components were achieved on a J'sphere ODS C(18) column (250 × 4.6 mm, 4 µm). The elution was done using water with 0.1% phosphoric acid (A) and acetonitrile with 0.1% phosphoric acid (B) in a two-step elution of the mobile phase at a flow rate of 1.0 mL/min and a wavelength of 230 nm. The method was validated for linearity, recovery, precision, accuracy, stability and robustness. All calibration curves showed good linear regression (r(2) > 0.999) within the test ranges. This method showed good recovery and reproducibility for the quantification of these four components in 85 species of Eucommiae cortex. The intra-day and inter-day precisions were lower than 0.53% (as a relative standard deviation, RSD) and accuracies between 93.00 and 106.28% for all standards. The results indicate that the established HPLC/UV method is suitable for quantitation and quality evaluation of Eucommiae cortex.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Republic of Korea.
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Republic of Korea.
authors.forename: Su Yang
authors.initials: SY
authors.lastname: Jeong
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Tae In
authors.initials: TI
authors.lastname: Kim
authors.affiliation: Division of Life and Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Seo
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Republic of Korea.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Republic of Korea. woomh@cu.ac.kr.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods / Q000592:standards*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D031283:Eucommiaceae; D057888:Iridoid Glucosides / Q000737:chemistry / Q000302:isolation & purification; D024301:Plant Bark; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000592:standards
chemical_list: D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; C058966:geniposidic acid
keywords: Eucommiae cortex; HPLC; Pattern recognition; Quality control