title: Simultaneous electrochemical magneto genosensing of foodborne bacteria based on triple-tagging multiplex amplification.
abstract: Simultaneous detection of Salmonella enterica, Listeria monocytogenes and Escherichia coli based on triple-tagging multiplex PCR and electrochemical magneto genosensing on silica magnetic particles is reported. A set of tagging primers were selected for the specific amplification of yfiR (375 bp), hlyA (234 bp) and eaeA (151bp), being one of the primers for each set labelled with fluorescein, biotin and digoxigenin coding for S. enterica, L. monocytogenes and E. coli, respectively. Afterwards, electrochemical magneto genosensing of the bacteria was achieved by using silica magnetic particles as a carrier and three different electrochemical reporters, specific for each pathogen. This method was able to clearly distinguish among the pathogenic bacteria tested within 50 min, with detection limits ranging from 12 to 46 pg μL(-1).
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain.
authors.forename: Delfina
authors.initials: D
authors.lastname: Brandão
authors.affiliation: Applied Enzyme Technology Ltd., Gwent Group Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UK.
authors.forename: Susana
authors.initials: S
authors.lastname: Liébana
authors.affiliation: Grup de Microbiologia Molecular, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Campoy
authors.affiliation: Grup de Microbiologia Molecular, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Cortés
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Alegret
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain. Electronic address: Isabel.Pividori@uab.CAT.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Pividori
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000295:instrumentation*; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D060306:Magnetometry / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D018511:Systems Integration
chemical_list: D045424:Complex Mixtures; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide
keywords: Electrochemical genosensing; Foodborne bacteria; Magneto-actuated electrode; Simultaneous detection; Triple-tagging multiplex PCR