title: Microarray based screening of peptide nano probes for HER2 positive tumor.
abstract: Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10(-8) M were obtained from a 10(5) library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.
authors.forename: Zihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Weizhi
authors.initials: W
authors.lastname: Wang
authors.forename: Xiangli
authors.initials: X
authors.lastname: Bu
authors.forename: Zewen
authors.initials: Z
authors.lastname: Wei
authors.forename: Lingling
authors.initials: L
authors.lastname: Geng
authors.affiliation: ‡Medical Isotopes Research Center, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing China, 100191.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
authors.affiliation: ‡Medical Isotopes Research Center, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing China, 100191.
authors.forename: Chengyan
authors.initials: C
authors.lastname: Dong
authors.affiliation: ‡Medical Isotopes Research Center, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing China, 100191.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.affiliation: ‡Medical Isotopes Research Center, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing China, 100191.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: §Institute for Systems Biology, 401 Terry Avenue North, Seattle, Washington 98109, United States.
authors.forename: Christopher
authors.initials: C
authors.lastname: Lausted
authors.affiliation: §Institute for Systems Biology, 401 Terry Avenue North, Seattle, Washington 98109, United States.
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
authors.affiliation: §Institute for Systems Biology, 401 Terry Avenue North, Seattle, Washington 98109, United States.|∥Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing China, 102206.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics; D045744:Cell Line, Tumor; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D051379:Mice; D046228:Microarray Analysis; D018613:Microscopy, Confocal; D036103:Nanotechnology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005456:Fluorescent Dyes; D018719:Erb-b2 Receptor Tyrosine Kinases