title: Aptamer-Modified Temperature-Sensitive Liposomal Contrast Agent for Magnetic Resonance Imaging.
abstract: A novel aptamer modified thermosensitive liposome was designed as an efficient magnetic resonance imaging probe. In this paper, Gd-DTPA was encapsulated into an optimized thermosensitive liposome (TSL) formulation, followed by conjugation with AS1411 for specific targeting against tumor cells that overexpress nucleolin receptors. The resulting liposomes were extensively characterized in vitro as a contrast agent. As-prepared TSLs-AS1411 had a diameter about 136.1 nm. No obvious cytotoxicity was observed from MTT assay, which illustrated that the liposomes exhibited excellent biocompatibility. Compared to the control incubation at 37 °C, the liposomes modified with AS1411 exhibited much higher T1 relaxivity in MCF-7 cells incubated at 42 °C. These data indicate that the Gd-encapsulated TSLs-AS1411 may be a promising tool in early cancer diagnosis.
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Kunchi
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tong
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Jine
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Hailu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and Nanobionics, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D019786:Gadolinium DTPA / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008081:Liposomes; D061986:MCF-7 Cells; D008422:Materials Testing; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011859:Radiography; D052156:SELEX Aptamer Technique
chemical_list: D003287:Contrast Media; D008081:Liposomes; D009363:Neoplasm Proteins; D010455:Peptides; C560441:nucleolin binding peptide; D019786:Gadolinium DTPA