title: Dynamic compression combined with exogenous SOX-9 promotes chondrogenesis of adipose-derived mesenchymal stem cells in PLGA scaffold.
abstract: OBJECTIVE
  Mechanical stimuli play a crucial role in cartilage repair and regeneration. Dynamic compression, as a physical stimulus, has been demonstrated to be an important factor in regulating the proliferation and differentiation of adipose-derived mesenchymal stem cells (ADSCs). However, the interaction of mechanical stimuli and chondrogenesis regulator on the chondrocyte phenotype and differentiation of ADSCs remains unknown. In the present study, we investigated the effects of dynamic compression combined with exogenous SOX-9 on chondrogenesis of ADSCs in a three-dimensional porous polylactic-co-glycolic acid (PLGA) scaffold.
  
  
  METHODS
  The morphology of ADSCs on the scaffolds was examined using scanning electron microscopy (SEM). The proliferation of ADSCs was evaluated by MTT assay. The expression of cartilage-specific genes in early chondrogenic differentiation was assessed by real-time PCR.
  
  
  RESULTS
  Our results indicated that the combination of dynamic compression with exogenous SOX-9 induces the expression of chondrogenic genes and promotes the proliferation of ADSCs.
  
  
  CONCLUSIONS
  Therefore, compression and SOX-9 have positive effects on chondrogenesis process of ADSCs, which may benefit articular cartilage regeneration.
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, China. Zhangye923088@sina.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: C-L
authors.initials: CL
authors.lastname: Tang
authors.forename: W-J
authors.initials: WJ
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: S-L
authors.initials: SL
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism; D020219:Chondrogenesis / Q000187:drug effects* / Q000502:physiology; D019245:Compressive Strength / Q000502:physiology; D019344:Lactic Acid / Q000008:administration & dosage*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D055758:SOX9 Transcription Factor / Q000096:biosynthesis / Q000494:pharmacology*; D054457:Tissue Scaffolds
chemical_list: D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid