title: Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with lipofectamine.
abstract: For the development of an effective nonviral gene vector, ternary complexes were prepared through the compaction of nanofiber-polyplexes. These were formed using pDNA and a head-tail type polycation bearing a multi-arm poly(ethylene glycol) head and a poly(l-lysine) tail, and this strategy was based on the crowding effect of poly(ethylene glycol) in the polyplex. Mixing was carried out using a cationic lipid (lipofectamine), which is a commercially available transfection reagent. Through ternary complex formation, the elongated morphology of nanofiber-polyplexes was found to compact into a spherical shape with an average diameter of ca. 100 nm. Accompanying ternary complex formation, the compaction of the nanofiber-polyplexes can improve cellular uptake and helps the ternary complex to retain its smooth transcription/translation process, which is characteristic of nanofiber-polyplexes. As a result, ternary complexes prepared at an optimal mixing ratio exhibit a high transfection efficiency compared with lipofectamine lipoplexes.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. harada@chem.osakafu-u.ac.jp.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Aono
authors.forename: Kenta
authors.initials: K
authors.lastname: Nomura
authors.forename: Eiji
authors.initials: E
authors.lastname: Yuba
authors.forename: Atsushi
authors.initials: A
authors.lastname: Harada
authors.forename: Kenji
authors.initials: K
authors.lastname: Kono
mesh_terms: D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000737:chemistry* / Q000235:genetics; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism; D057139:Nanofibers; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014162:Transfection
chemical_list: D004247:DNA; D008055:Lipids; D008239:Lysine; D011073:Polyamines; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols; D011108:Polymers; C086724:Lipofectamine