title: The quantitative changes in the yeast Hsp70 and Hsp90 interactomes upon DNA damage.
abstract: The molecular chaperones Hsp70 and Hsp90 participate in many important cellular processes, including how cells respond to DNA damage. Here we show the results of applied quantitative affinity-purification mass spectrometry (AP-MS) proteomics to understand the protein network through which Hsp70 and Hsp90 exert their effects on the DNA damage response (DDR). We characterized the interactomes of the yeast Hsp70 isoform Ssa1 and Hsp90 isoform Hsp82 before and after exposure to methyl methanesulfonate. We identified 256 chaperone interactors, 146 of which are novel. Although the majority of chaperone interaction remained constant under DNA damage, 5 proteins (Coq5, Ast1, Cys3, Ydr210c and Rnr4) increased in interaction with Ssa1 and/or Hsp82. This data presented here are related to [1] (Truman et al., in press). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository (Vizcaino et al. (2013) [2]) with the dataset identifier PXD001284.
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Truman
authors.affiliation: Department of Biomedical Sciences, Midwestern University, Downers Grove, IL 60515, USA.
authors.forename: Kolbrun
authors.initials: K
authors.lastname: Kristjansdottir
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Wolfgeher
authors.affiliation: Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Barcelona, Catalunya, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ricco
authors.affiliation: Computation Institute, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Anoop
authors.initials: A
authors.lastname: Mayampurath
authors.affiliation: Computation Institute, The University of Chicago, Chicago, IL 60637, USA ; Department of Pediatrics, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Samuel L
authors.initials: SL
authors.lastname: Volchenboum
authors.affiliation: Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Barcelona, Catalunya, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Clotet
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Kron