title: Mycoplasma pneumoniae and Chlamydophila pneumoniae: a comparative study in patients with nasal polyposis and healthy controls.
abstract: BACKGROUND
  The role played by Mycoplasma pneumoniae and Chlamydophila pneumoniae in the pathogenesis of chronic rhinosinusitis with nasal polyps has been the object of ongoing debate. We used real-time polymerase chain reaction to investigate the prevalence of both microorganisms in the nasal tissue samples of patients and controls.
  
  
  METHODS
  We extracted DNA from nasal polyp samples obtained during functional endoscopic sinus surgery and the inferior turbinate samples of controls undergoing septoplasty. We used the highly sensitive real-time polymerase chain reaction to detect the presence of M pneumoniae and C pneumoniae DNA.
  
  
  RESULTS
  Patients with chronic rhinosinusitis with nasal polyps consisted of 62 individuals (39 men; mean age 51 years); the control group consisted of 24 individuals (13 men; mean age 45 years). All samples from both groups were negative for M pneumoniae and C pneumoniae DNA.
  
  
  CONCLUSIONS
  We have demonstrated that the likelihood of M pneumoniae and C pneumoniae acting as an ongoing inflammatory stimulus in chronic rhinosinusitis with nasal polyps is slim.
authors.affiliation: Department of Microbiology,University of Thessaly,Larissa,Greece.
authors.forename: D G
authors.initials: DG
authors.lastname: Ioannidis
authors.affiliation: Department of Otorhinolaryngology,University Hospital of Larissa,Greece.
authors.forename: V A
authors.initials: VA
authors.lastname: Lachanas
authors.affiliation: Department of Microbiology,University of Thessaly,Larissa,Greece.
authors.forename: Z
authors.initials: Z
authors.lastname: Florou
authors.affiliation: Department of Otorhinolaryngology,University Hospital of Larissa,Greece.
authors.forename: J G
authors.initials: JG
authors.lastname: Bizakis
authors.affiliation: Department of Microbiology,University of Thessaly,Larissa,Greece.
authors.forename: E
authors.initials: E
authors.lastname: Petinaki
authors.affiliation: Department of Otorhinolaryngology,University Hospital of Larissa,Greece.
authors.forename: C E
authors.initials: CE
authors.lastname: Skoulakis
mesh_terms: D000328:Adult; D023521:Chlamydophila Infections / Q000175:diagnosis* / Q000453:epidemiology*; D016993:Chlamydophila pneumoniae; D002908:Chronic Disease; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009177:Mycoplasma pneumoniae; D009298:Nasal Polyps / Q000175:diagnosis* / Q000453:epidemiology*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000453:epidemiology*; D060888:Real-Time Polymerase Chain Reaction; D012220:Rhinitis / Q000175:diagnosis* / Q000453:epidemiology*; D012852:Sinusitis / Q000175:diagnosis* / Q000453:epidemiology*
keywords: Chlamydophila pneumoniae; Mycoplasma pneumoniae; Nasal Polyps; Real-Time Polymerase Chain Reaction