title: P27 expression in pleomorphic salivary gland adenoma.
abstract: OBJECTIVE
  This study aims to characterise alterations in the immunohistochemical expression of p27 in normal tissue of the salivary gland surrounding pleomorphic adenoma and in the tumour cells of pleomorphic adenoma.
  
  
  METHODS
  A retrospective study of data including 120 cases of pleomorphic adenomas (66 female, 54 male) retrieved from the files of the Oral Pathology Departments of Aleppo University, Syria and Al-Farabi Dental and Nursing College. Immunohistochemical expression against p27 was examined in the selected cases. The percentage of p27-positive nuclei was semi-quantitatively assessed by two independent observers and scores were given. The statistical analysis included the use of descriptive statistics and proportional frequencies.
  
  
  RESULTS
  The results showed that P27 nuclear staining with high staining (moderate to strong positive staining in more than 50% of nuclei, depending on the percentage of nuclei for the cells) was noted in tumour duct cells of pleomorphic adenoma in 90 (75%) cases out of 120, while 30 (25%) cases showed low staining (positive staining in less than 5% of nuclei, depending on the percentage of nuclei for the cells).
  
  
  CONCLUSIONS
  Our data suggests that p27 might have a minor role in the development of pleomorphic adenoma.
authors.affiliation: Department of Oral Maxillofacial Sciences, Al-Farabi College of Dentistry and Nursing, Riyadh, Saudi Arabia.
authors.forename: Bassel
authors.initials: B
authors.lastname: Tarakji
authors.affiliation: Department of Oral Maxillofacial Sciences, Al-Farabi College of Dentistry and Nursing, Riyadh, Saudi Arabia.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Umair
authors.affiliation: Department of Restorative Dentistry, Al-Farabi College of Dentistry and Nursing Riyadh, Saudi Arabia.
authors.forename: Mohammed Alsakran
authors.initials: MA
authors.lastname: Altamimi
authors.affiliation: Department of Oral Maxillofacial Sciences, Al-Farabi College of Dentistry and Nursing, Riyadh, Saudi Arabia.
authors.forename: Saleh Nasser
authors.initials: SN
authors.lastname: Azzeghaiby
authors.affiliation: Department of Oral Maxillofacial Sciences, Al-Farabi College of Dentistry and Nursing, Riyadh, Saudi Arabia.
authors.forename: Abla Sayed
authors.initials: AS
authors.lastname: Mahmoud
authors.affiliation: Department of Oral Maxillofacial Sciences, Al-Farabi College of Dentistry and Nursing, Riyadh, Saudi Arabia.
authors.forename: Shourouk
authors.initials: S
authors.lastname: Darwish
authors.affiliation: Department of Restorative Dentistry, Al-Farabi College of Dentistry and Nursing Riyadh, Saudi Arabia.
authors.forename: Mohammad Zakaria
authors.initials: MZ
authors.lastname: Nassani
authors.affiliation: Department of Oral Maxillofacial Sciences, Al-Farabi College of Dentistry and Nursing, Riyadh, Saudi Arabia.
authors.forename: Nipun
authors.initials: N
authors.lastname: Ashok
keywords: immunostaining; nuclear staining; p27 expression; pleomorphic adenoma; tumour suppressor gene