title: Galactosylated magnetic nanovectors for regulation of lipid metabolism based on biomarker-specific RNAi and MR imaging.
abstract: The specific delivery of ribonucleic acid (RNA) interfering molecules to disease-related cells is still a critical blockade for in vivo systemic treatment. Here, this study suggests a robust delivery carrier for targeted delivery of RNA-interfering molecules using galactosylated magnetic nanovectors (gMNVs). gMNVs are an organic-inorganic polymeric nanomaterial composed of polycationics and magnetic nanocrystal for delivery of RNA-interfering molecules and tracking via magnetic resonance (MR) imaging. In particular, the surface of gMNVs was modified by galactosylgluconic groups for targeted delivering to asialoglycoprotein receptor (ASGPR) of hepatocytes. Moreover, the small interfering RNAs were used to regulate target proteins related with low-density lipoprotein level and in vivo MR imaging was conducted for tracking of nanovectors. The obtained results show that the prepared gMNVs demonstrate potential as a systemic theragnostic nanoplatform for RNA interference and MR imaging.
authors.affiliation: Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Korea. Nanomedical National Core Research Center, Yonsei University, Seoul 120-749, Korea.
authors.forename: Dan
authors.initials: D
authors.lastname: Heo
authors.forename: Chanjoo
authors.initials: C
authors.lastname: Lee
authors.forename: Minhee
authors.initials: M
authors.lastname: Ku
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Sahng Wook
authors.initials: SW
authors.lastname: Park
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005690:Galactose / Q000737:chemistry*; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D034622:RNA Interference / Q000187:drug effects*
chemical_list: D037263:Asialoglycoprotein Receptor; D058185:Magnetite Nanoparticles; D005690:Galactose