title: Asp3Gly polymorphism affects fatty acid-binding protein 3 intracellular stability and subcellular localization.
abstract: Fatty acid-binding proteins (FABP) play a crucial role in intracellular fatty acid transportation and metabolism. In this study, we investigate the effects of the FABP3 Asp3Gly (D3G) polymorphism on protein structure and function. Although the mutation did not alter protein secondary structure or the ability to bind 1-anilinonaphthalene-8-sulfonic acid and palmitate, the intracellular stability of the D3G mutant was significantly decreased. Immunocytochemical analysis reveals that the mutation alters FABP3 subcellular localization. Our results suggest that the D3G polymorphism may impact energy metabolism and physiological functions.
authors.affiliation: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai 487-8501, Japan. Electronic address: kusudo@isc.chubu.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kusudo
authors.affiliation: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Hashida
authors.affiliation: Department of Health and Medical Sciences, Aichi Shukutoku University, Nagakute 480-1197, Japan.
authors.forename: Fujiko
authors.initials: F
authors.lastname: Ando
authors.affiliation: Graduate School of Nutritional Sciences, Nagoya University of Arts and Sciences, Nisshin 470-0196, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimokata
authors.affiliation: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai 487-8501, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002460:Cell Line; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000166:cytology; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D011487:Protein Conformation; D055550:Protein Stability; D021381:Protein Transport
chemical_list: C493408:FABP3 protein, human; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids
keywords: Fatty acid binding protein; Polymorphism; Protein stability; Single-nucleotide polymorphism; Subcellular localizatoin