title: Steviol glycosides targeted analysis in leaves of Stevia rebaudiana (Bertoni) from plants cultivated under chilling stress conditions.
abstract: Stevia rebaudiana is an important agricultural crop for the production of a high-potency natural sweetener, sensitive to low temperature during the developmental stage. Stimulation of chilling stress with a pre-treatment with endogenous signalling components and in particular with salicylic acid (SA), hydrogen peroxide (H2O2), 6-benzylaminopurine (BAP) and calcium chloride (CaCl2) could induce tolerance to chilling and could constitute a suitable way to maintain quality and quantity of steviol glycosides under controlled artificial environment. In the present work the effects of different putative signalling molecules on the morpho-physiological parameters were evaluated, and a specific method for the quali-quantitative analysis of steviol glycosides in S. rebaudiana plants cultivated under controlled conditions was developed, by using LC-ESI-FT (Orbitrap) MS, LC-ESI-QqQ-MS/MS and multivariate data analysis. This approach underlined that the pre-treatment has influence on the production of secondary metabolites. In particular Stevia plants characterised by higher contents of rebaudioside A and stevioside, were identified.
authors.affiliation: Département de Production Végétale, Institut National Agronomique de Tunisie, 43, Avenue Charles Nicolle, 1082 Tunis, Mahrajène, Tunisia. Electronic address: sihemsoufi@yahoo.fr.
authors.forename: Sihem
authors.initials: S
authors.lastname: Soufi
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
authors.forename: Gilda
authors.initials: G
authors.lastname: D'Urso
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.affiliation: Département de Production Végétale, Institut National Agronomique de Tunisie, 43, Avenue Charles Nicolle, 1082 Tunis, Mahrajène, Tunisia.
authors.forename: Salah
authors.initials: S
authors.lastname: Rezgui
authors.affiliation: Département de Production Végétale, Institut National Agronomique de Tunisie, 43, Avenue Charles Nicolle, 1082 Tunis, Mahrajène, Tunisia.
authors.forename: Taoufik
authors.initials: T
authors.lastname: Bettaieb
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy. Electronic address: pmontoro@unisa.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003080:Cold Temperature; D045786:Diterpenes, Kaurane / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D025341:Principal Component Analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D045786:Diterpenes, Kaurane; D010936:Plant Extracts; C012042:steviol
keywords: Chlorophyll fluorescence; LC–ESI-FT (Orbitrap) MS; LC–ESI–QqQ-MS/MS; Principal component analysis; Steviol glycosides