title: Effect of bone marrow-derived stem cells and bone morphogenetic protein-2 on treatment of osteoradionecrosis in a rat model.
abstract: OBJECTIVE
  We investigated the effect of rat mesenchymal stem cells (rMSCs) and bone morphogenetic protein-2 (BMP-2) on the osseous healing of osteoradionecrosis in the rat mandible, depending on application time.
  
  
  METHODS
  One week after irradiation (30 Gy) on the right mandible, all molar teeth were extracted. In Group 1 (n = 25), rMSCs and/or BMP-2 carried with hydrogel were applied immediately after surgery. In Group 2 (n = 25), application was done 4 weeks after surgery, after the occurrence of osteoradionecrosis. No hydrogel (n = 5), hydrogel alone (n = 5), hydrogel with 2 × 10(4) rMSCs (n = 5), hydrogel with 10 μg of BMP-2 (n = 5), or hydrogel with 10 μg of BMP-2 and 2 × 10(4) rMSCs (n = 5) was applied. Micro-CT data of bone healing were collected at 4 weeks post-application.
  
  
  RESULTS
  In Group 1, BMP-2 was effective in increasing both bone volume (BV) and bone mineral density (BMD), whereas rMSCs were not. In Group 2, the combined application of rMSCs and BMP-2 significantly increased BMD and BV compared to values in the groups without hydrogel and with hydrogel alone.
  
  
  CONCLUSIONS
  Osseous healing after post-irradiation trauma in rats was enhanced immediately after dentoalveolar trauma by application of BMP-2, whereas the combined application of rMSCs and BMP-2 was most effective after osteoradionecrosis occurred.
authors.affiliation: Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Brain Korea 21 Plus, Dental Research Institute, Seoul National University, Seoul, 110-768, Republic of Korea.
authors.forename: Im Geon
authors.initials: IG
authors.lastname: Jin
authors.affiliation: Dept. of Radiation Oncology, Seoul National University Hospital, Seoul, 110-744, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Dept. of Radiation Oncology, Seoul National University Hospital, Seoul, 110-744, Republic of Korea.
authors.forename: Hong-Gyun
authors.initials: HG
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, Medical College, Korea University, Seoul, Republic of Korea.
authors.forename: Sook Kyoung
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Medical College, Korea University, Seoul, Republic of Korea.
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Park
authors.affiliation: Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Brain Korea 21 Plus, Dental Research Institute, Seoul National University, Seoul, 110-768, Republic of Korea. Electronic address: sjhwang@snu.ac.kr.
authors.forename: Soon Jung
authors.initials: SJ
authors.lastname: Hwang
mesh_terms: D000818:Animals; D015519:Bone Density / Q000502:physiology; D055396:Bone Morphogenetic Protein 2 / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D004337:Drug Carriers; D006820:Hyaluronic Acid / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D008297:Male; D008336:Mandibular Diseases / Q000188:drug therapy / Q000628:therapy*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009929:Organ Size; D010025:Osteoradionecrosis / Q000188:drug therapy / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013529:Surgical Wound Dehiscence / Q000188:drug therapy / Q000628:therapy; D054457:Tissue Scaffolds / Q000737:chemistry; D014081:Tooth Extraction; D020390:Tooth Socket / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D004337:Drug Carriers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid
keywords: Bone marrow-derived stem cell; Bone morphogenetic protein-2; Osteoradionecrosis