title: Fixation of Light Weight Polypropylene Mesh with n-Butyl-2-cyanocrylate in Pelvic Floor Surgery: Experimental Design Approach in Sheep for Effectiveness Evaluation.
abstract: OBJECTIVE
  The aim of this study was to find a proper experimental design and to evaluate n-butyl-2-cyanoacrylate (Histoacryl) as a fixation method for a light-weight and large pore PP mesh (Synthetic PP Mesh-1) using the sheep as an animal model.
  
  
  METHODS
  Posterior vaginal implantation by means of episiotomy was used to implant 8 ewes which were evaluated macroscopically and histologically at 3 months (n = 4) and 6 months (n = 4) post-surgery. In previous pilot studies anterior vaginal implantation was evaluated, as well as different synthetic mesh materials, sizes and fixation methods (n = 1 to 3) during three weeks. In all cases a clinical evaluation of the animal was performed.
  
  
  RESULTS
  A reduction in the mesh size (Synthetic PP Mesh-1) together with precise application of the surgical glue Histoacryl to fix the mesh yielded significantly better histocompatibility results (P < 0.01) compared to larger size or other fixation methods.
  
  
  CONCLUSIONS
  The combination of Synthetic PP Mesh-1 with Histoacryl offered a high degree of graft integration without vaginal ulceration and a minimal foreign body reaction, being the sheep a proper animal model to test these types of medical devices.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain ; Integrated Services of Laboratory Animals, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Barbosa
authors.affiliation: B. Braun Surgical S.A., Rubi, 08191 Barcelona, Spain.
authors.forename: Tania
authors.initials: T
authors.lastname: Nieves
authors.affiliation: Department of Animal Medicine and Surgery, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: García
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain ; Integrated Services of Laboratory Animals, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Cepeda
authors.affiliation: Department of Animal Medicine and Surgery, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Moll
authors.affiliation: Department of Animal Health and Anatomy, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Marco
authors.affiliation: B. Braun Surgical S.A., Rubi, 08191 Barcelona, Spain.
authors.forename: Christine
authors.initials: C
authors.lastname: Weis
authors.affiliation: B. Braun Surgical S.A., Rubi, 08191 Barcelona, Spain.
authors.forename: Pau
authors.initials: P
authors.lastname: Turon
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain ; Integrated Services of Laboratory Animals, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain.
authors.forename: Patri
authors.initials: P
authors.lastname: Vergara
mesh_terms: D000818:Animals; D004659:Enbucrilate / Q000008:administration & dosage*; D004841:Episiotomy; D005260:Female; D006801:Humans; D023421:Models, Animal; D017773:Pelvic Floor / Q000473:pathology / Q000601:surgery*; D012756:Sheep; D013526:Surgical Mesh; D014621:Vagina / Q000473:pathology / Q000601:surgery*
chemical_list: D004659:Enbucrilate