title: Anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from traditional medicinal plant, Costus speciosus (Koen ex.Retz.) Sm.
abstract: Costus speciosus is an important medicinal plant widely used in several indigenous medicinal formulations. The present study was conducted to evaluate the in vitro anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from C. speciosus. The diosgenin was isolated from C. speciosus by HPTLC and its biological activities were studied by different protocols. The results demonstrated that LPS stimulated TNF-α generation in RAW 264.7 macrophage culture supernatant up to 3.7-fold of the control and that sample treatment (50 μg/mL) resulted in a highly significant inhibitory effect on LPS-stimulated TNF-α (p < 0.01) in a similar manner to methotrexate inhibitory effect. The tested sample possessed an effective antioxidant scavenging affinity against DPPH radicals as compared with the standard antioxidant activity of vitamin C. The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer, apoptotic and inhibitory effects on cell proliferation.
authors.affiliation: a Department of Clinical Laboratory Sciences , College of Applied Medical Sciences, Aljouf University , Sakaka , Saudi Arabia.|c Faculty of Science, Microbiology and Botany Department , Suez Canal University , Ismailia , Egypt.
authors.forename: Samy
authors.initials: S
authors.lastname: Selim
authors.affiliation: b YAJ Prophetic Medicine Application , College of Medicine, King Abdulaziz University , Jeddah , Saudi Arabia.
authors.forename: Soad
authors.initials: S
authors.lastname: Al Jaouni
mesh_terms: D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D029770:Costus / Q000737:chemistry*; D004144:Diosgenin / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D004144:Diosgenin
keywords: Anti-inflammatory; C. speciosus; anti-angiogenic; antioxidant; diosgenin