title: Altered microRNAs expression profiling in cumulus cells from patients with polycystic ovary syndrome.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age, and oocyte developmental competence is altered in patients with PCOS. In recent years microRNAs (miRNAs) have emerged as important regulators of gene expression, the aim of the study was to study miRNAs expression patterns of cumulus cells from PCOS patients.
  
  
  METHODS
  The study included 20 patients undergoing in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI): 10 diagnosed with PCOS and 10 matching controls. We used deep sequencing technology to identify the miRNAs differentially expressed in the cumulus cells of PCOS.
  
  
  RESULTS
  There were 17 differentially expressed miRNAs in PCOS cumulus cells, including 10 miRNAs increase and 7 miRNAs decrease. These miRNAs were predicted to target a large set of genes with different functions, including Wnt- and MAPK- signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation and cell cycle. Unsupervised hierarchical clustering analysis demonstrated that there was a specific miRNAs expression pattern in PCOS cumulus cells.
  
  
  CONCLUSIONS
  We found that the miRNAs expression profile was different in cumulus cells isolated from PCOS patients compared with control. This study provided new evidence for understanding the pathogenesis of PCOS.
authors.affiliation: Department of In-Vitro Fertilization, Shanghai Zhongshan Hospital, Shanghai, China. lsy6592@163.com.
authors.forename: Suying
authors.initials: S
authors.lastname: Liu
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. zhangxuancw@outlook.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. lincomycine@hotmail.com.
authors.forename: Changgen
authors.initials: C
authors.lastname: Shi
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. jimin.lin@gmail.com.
authors.forename: Jimin
authors.initials: J
authors.lastname: Lin
authors.affiliation: Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai Jiai Genetics and IVF Institute, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China. chengw911@qq.com.|Medical School of Fudan University, Shanghai, China. chengw911@qq.com.
authors.forename: Guowu
authors.initials: G
authors.lastname: Chen
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. 462576747@qq.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. lgwu@sibcb.ac.cn.
authors.forename: Ligang
authors.initials: L
authors.lastname: Wu
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. shihuijuan2011@163.com.|Medical School of Fudan University, Shanghai, China. shihuijuan2011@163.com.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Shi
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. yuanyao60@126.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. zw0822@sina.com.|Medical School of Fudan University, Shanghai, China. zw0822@sina.com.
authors.forename: Weijin
authors.initials: W
authors.lastname: Zhou
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. sunzgbio@126.com.|Medical School of Fudan University, Shanghai, China. sunzgbio@126.com.
authors.forename: Zhaogui
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of In-Vitro Fertilization, Shanghai Zhongshan Hospital, Shanghai, China. dongxi222@hotmail.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Dong
authors.affiliation: National Populations and Family Planning Key Laboratory of Contraceptive Drugs and Devices, The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China. wangjiansippr@126.com.|Medical School of Fudan University, Shanghai, China. wangjiansippr@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016000:Cluster Analysis; D019295:Computational Biology; D054885:Cumulus Cells / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020935:MAP Kinase Signaling System; D008540:Meiosis; D035683:MicroRNAs / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D015398:Signal Transduction; D020554:Sperm Injections, Intracytoplasmic; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D051153:Wnt Proteins; D011374:Progesterone