title: Immunoblotting using Strongyloides venezuelensis larvae, parthenogenetic females or eggs extracts for the diagnosis of experimentally infected immunosuppressed rats.
abstract: The nematode Strongyloides stercoralis is responsible for strongyloidiasis in humans. Diagnosis of infection occurs through detection of larvae in feces, but low elimination of larvae often hampers the detection of disease, particularly in cases of patient immunosuppression. Immunodiagnostic tests have been developed; however obtaining S. stercoralis larvae for the production of homologous antigen extract is technically difficult. Thus, the use different developmental forms of Strongyloides venezuelensis has become an alternative method for the production of antigen extracts. The aim of this study was to evaluate immunoblotting using alkaline extracts from S. venezuelensis L3 larvae, parthenogenetic females or eggs to test detection of experimental strongyloidiasis associated with immunosuppression. Immunocompetent and immunosuppressed male rats were experimentally infected, and serum sample from all animals were obtained at 0, 5, 8 13, and 21 days post infection (d.p.i.). Immunoblotting was evaluated for use in detection of anti-S. venezuelensis IgG in both experimental rat groups. The larval extract immunoblotting profile had the most immunoreactive fractions in the immunosuppressed group beginning at 5 d.p.i., while the immunocompetent group reactivity began on 8 d.p.i. Immunoreactive protein fractions of 17 kDa present in larval alkaline extract presented as possible markers of infection in immunosuppressed rats. It is concluded that all extracts using immunoblotting have diagnostic potential in experimental strongyloidiasis, particularly larval extract in immunosuppressed individuals.
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, CEP 38400-902, Uberlândia, MG, Brazil.
authors.forename: Edson Fernando
authors.initials: EF
authors.lastname: Goulart de Carvalho
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, CEP 38400-902, Uberlândia, MG, Brazil.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Neto de Sousa
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, CEP 38400-902, Uberlândia, MG, Brazil.
authors.forename: Ana Lúcia Ribeiro
authors.initials: AL
authors.lastname: Gonçalves
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, CEP 38400-902, Uberlândia, MG, Brazil.
authors.forename: Jair Pereira
authors.initials: JP
authors.lastname: da Cunha-Junior
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, CEP 38400-902, Uberlândia, MG, Brazil. Electronic address: costacruz@ufu.br.
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000469:parasitology; D005260:Female; D015151:Immunoblotting / Q000379:methods*; D007121:Immunocompetence; D016867:Immunocompromised Host; D007074:Immunoglobulin G / Q000097:blood; D007814:Larva; D008297:Male; D010063:Ovum; D010312:Parthenogenesis; D051381:Rats; D017208:Rats, Wistar; D013321:Strongyloides / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G
keywords: Immunoblotting; Immunossupression; Rats; Strongyloides venezuelensis