title: A Murine Myh6MerCreMer Knock-In Allele Specifically Mediates Temporal Genetic Deletion in Cardiomyocytes after Tamoxifen Induction.
abstract: A mouse model that mediates temporal, specific, and efficient myocardial deletion with Cre-LoxP technology will be a valuable tool to determine the function of genes during heart formation. Mhy6 encodes a cardiac muscle specific protein: alpha-myosin heavy chain. Here, we generated a new Myh6-MerCreMer (Myh6(MerCreMer/+)) inducible Cre knock-in mouse by inserting a MerCreMer cassette into the Myh6 start codon. By crossing knock-in mice with Rosa26 reporter lines, we found the Myh6(MerCreMer/+) mice mediate complete Cre-LoxP recombination in cardiomyocytes after tamoxifen induction. X-gal staining and immunohistochemistry analysis revealed that Myh6-driven Cre recombinase was specifically activated in cardiomyocytes at embryonic and adult stages. Furthermore, echocardiography showed that Myh6(MerCreMer/+) mice maintained normal cardiac structure and function before and after tamoxifen administration. These results suggest that the new Myh6(MerCreMer/+) mouse can serve as a robust tool to dissect the roles of genes in heart development and function. Additionally, myocardial progeny during heart development and after cardiac injury can be traced using this mouse line.
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
authors.forename: Nishat
authors.initials: N
authors.lastname: Sultana
authors.affiliation: Leon H. Charney Division of Cardiology, New York University School of Medicine, New York, NY, United States of America.
authors.forename: David S
authors.initials: DS
authors.lastname: Park
authors.affiliation: Leon H. Charney Division of Cardiology, New York University School of Medicine, New York, NY, United States of America.
authors.forename: Akshay
authors.initials: A
authors.lastname: Shekhar
authors.affiliation: Leon H. Charney Division of Cardiology, New York University School of Medicine, New York, NY, United States of America.
authors.forename: Lei
authors.initials: L
authors.lastname: Bu
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
authors.forename: Shegufta
authors.initials: S
authors.lastname: Razzaque
authors.affiliation: Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
authors.forename: Chen-Leng
authors.initials: CL
authors.lastname: Cai
mesh_terms: D000483:Alleles; D000818:Animals; D017353:Gene Deletion; D019426:Integrases; D051379:Mice; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D013629:Tamoxifen / Q000494:pharmacology; D024723:Ventricular Myosins / Q000235:genetics* / Q000378:metabolism
chemical_list: D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases; D024723:Ventricular Myosins; D018995:Myosin Heavy Chains