title: What if cell culture media do not mimic in vivo redox settings?
abstract: Here, I address the topic of suitability for redox research of common settings in cell cultures. This is done through the prism of in vitro anticancer effects of vitamin C. Cell culture media show lower concentrations of iron and a higher level of oxygen compared to interstitial fluid. Such a setup promotes ascorbate-mediated production and accumulation of hydrogen peroxide, which efficiently kills a variety of cancer cell lines. However, the anticancer effects are annihilated if the iron level is corrected to mimic in vivo concentrations. It appears that the potential benefits of application of vitamin C in cancer treatment have been significantly overestimated. This might be true for other pro-oxidative agents as well, such as some (poly)phenols. We urgently need to establish medium formula and culture maintenance settings that are optimal for redox research.
authors.affiliation: a Department of Life Sciences, Institute for Multidisciplinary Research , University of Belgrade , Belgrade, Kneza Višeslava 1, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Spasojević
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003470:Culture Media; D007501:Iron; D001205:Ascorbic Acid
keywords: Ascorbate; Cancer; Cell culture; Iron