title: Actinotetraose L, a new tetrasaccharide derivative isolated from the mycelia of Amycolatopsis sp. HCa1.
abstract: A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]- β-D-glucopyranosyl-(1 → 2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1 → 2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed.
authors.affiliation: a Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences , Haikou 571101 , China.
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Guo
authors.affiliation: b Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093 , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: b Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093 , China.
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Tan
authors.affiliation: b Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , Nanjing 210093 , China.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Ge
mesh_terms: D000192:Actinomycetales / Q000737:chemistry*; D000818:Animals; D006110:Grasshoppers / Q000382:microbiology; D006801:Humans; D053565:Hypercalciuria; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D007166:Immunosuppressive Agents; D009844:Oligosaccharides; C000605697:actinotetraose L
keywords: Amycolatopsis; Pseudonocardiaceae; grasshopper; tetrasaccharide