title: Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces.
abstract: Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.
authors.forename: Silvana
authors.initials: S
authors.lastname: Carnevale
authors.forename: María Laura
authors.initials: ML
authors.lastname: Pantano
authors.forename: Laura
authors.initials: L
authors.lastname: Kamenetzky
authors.forename: Jorge Bruno
authors.initials: JB
authors.lastname: Malandrini
authors.forename: Claudia Cecilia
authors.initials: CC
authors.lastname: Soria
authors.forename: Jorge Néstor
authors.initials: JN
authors.lastname: Velásquez
mesh_terms: D000818:Animals; D003429:Cross Reactions; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000235:genetics; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D005243:Feces / Q000469:parasitology*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000469:parasitology*
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV