title: Investigation on effects of the nourishing kidney and eliminating toxicity decoction on immunological imbalance of Th1, Th17 and Treg in HBV transgenic mice.
abstract: OBJECTIVE
  To study the immune mechanism of nourishing kidney and eliminating toxicity decoction (NKETD) on Chronic Hepatitis B (CHB), we detected the serum concentrations of IFN-γ (the characteristic cytokine of Th1), IL-17A (the characteristic cytokine of Th17) and the quantitative proportion of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg in HBV transgenic mice.
  
  
  METHODS
  The HBV transgenic mice were randomly divided into six groups: high-dose group, middle-dose group, low-dose group, lamivudine group, model control group and normal mice control group. The serum concentrations of IFN-γ and IL-17A in mice were measured by ELISA method and the ratio of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg was detected by Flow Cytometry Method (FCM).
  
  
  RESULTS
  The decoction could increase the serum concentration of IFN-γ and decrease that of IL-17A in HBV transgenic mice. The higher the dose was, the more significantly the concentration of IFN-γ increased. And high-dose decoction could decrease the serum concentration of IL-17A in HBV transgenic mice significantly and continuously while middle-dose and low-dose decoction had no significant effects. However, there wasn't statistically significant variation on the ratio of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg in HBV transgenic mice.
  
  
  CONCLUSIONS
  The decoction could treat CHB by regulating the immune function by promoting the generation of Th1 and/or enhancing its function while inhibiting Th17. The immune regulation by decoction had more significant effects than lamivudine.
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
authors.forename: Qiu-Ying
authors.initials: QY
authors.lastname: Xu
authors.affiliation: ChangGang Street Community Health Service Center Haizhu District, Guangzhou 510280, China.
authors.forename: Jun-Xian
authors.initials: JX
authors.lastname: Chen
authors.affiliation: Tropical Medicine Institute, Guangzhou University of Chinese Medicine Guangzhou 510405, China.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
authors.forename: Hao-Jun
authors.initials: HJ
authors.lastname: Peng
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
authors.forename: Zheng-Lun
authors.initials: ZL
authors.lastname: Zeng
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Feng
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shen
keywords: Chronic Hepatitis B; IFN-γ; IL-17A; Nourishing kidney and eliminating toxicity decoction; ratio of CD+4CD+25 foxp3 Treg to CD+4 Treg