title: Quantitative PCR detection of feline morbillivirus in cat urine samples.
abstract: Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Furuya
authors.forename: Akiko
authors.initials: A
authors.lastname: Wachi
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sassa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Ryuji
authors.initials: R
authors.lastname: Fukushima
authors.forename: Makoto
authors.initials: M
authors.lastname: Shibutani
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Kinji
authors.initials: K
authors.lastname: Shirota
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000652:urine / Q000821:virology*; D002415:Cats; D018103:Morbillivirus / Q000302:isolation & purification*; D018185:Morbillivirus Infections / Q000652:urine / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*