title: Downregulation of miR-148b as biomarker for early detection of hepatocellular carcinoma and may serve as a prognostic marker.
abstract: MicroRNAs (miRNAs) have a large number of various target genes in different cancer types, which may result in many biological functions. Thus, identifying the molecular mechanisms of miRNAs may effect on the complexity of cancer progression via regulation of gene. In the current study, we utilized real-time PCR to quantify the diction of miR-148b in trail hepatocellular carcinoma (HCC) specimen and normal tissues. Furthermore, we evaluated the relationship of miR-148b and clinicopathological features with survival of HCC patients. Therefore, we evaluated the level of miR-148b expression in 101 HCC patients and also in 40 normal control cases. The result suggested lower expression in tumor tissues than normal control tissues (0.96 ± 0.14; 1.84 ± 0.20, P < 0.05). Our findings suggest that the declined expression of miR-148b can considerably be linked to tumor node metastasis (TNM) stage (stages III and IV; P = 0.021) and vein invasion (P = 0.029). Nevertheless, miR-148b expression was not related to sex (P = 0.674), age (P = 0. 523), size of tumor (P = 0.507), liver cirrhosis, and histologic grade (P = 0.734). Survival analysis showed that low expression was remarkably related to overall survival (P = 0.012). Furthermore, multivariate survival test suggested that decline of miR-148b diction was linked to poor survival in HCC patients. Our results suggested that miR-148b is decreased in HCC. Therefore, we concluded that miR-148b may play its role in the prognosis of HCC.
authors.affiliation: Department of Pathology, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Katayoun
authors.initials: K
authors.lastname: Ziari
authors.affiliation: Center for Chemical Biology, Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad, India.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Zarea
authors.affiliation: Department of Radiology, Medical Imaging Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Gity
authors.affiliation: Department of Legal Medicine, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Amir Farshid
authors.initials: AF
authors.lastname: Fayyaz
authors.affiliation: Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.affiliation: Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran.
authors.forename: Ebrahim Khodaverdi
authors.initials: EK
authors.lastname: Darian
authors.affiliation: Department of Emergency Medicine, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. hashemianam@mums.ac.ir.
authors.forename: Amir Masoud
authors.initials: AM
authors.lastname: Hashemian
keywords: Cancer; Liver; PCR; Pathology; miRNA