title: Data set for the proteomics analysis of the endomembrane system from the unicellular Entamoeba histolytica.
abstract: Entamoeba histolytica is the protozoan parasite agent of amebiasis, an infectious disease of the human intestine and liver. This parasite contact and kills human cells by an active process involving pathogenic factors. Cellular traffic and secretion activities are poorly characterized in E. histolytica. In this work, we took advantage of a wide proteomic analysis to search for principal components of the endomembrane system in E. histolytica. A total of 5683 peptides matching with 1531 proteins (FDR of 1%) were identified which corresponds to roughly 20% of the total amebic proteome. Bioinformatics investigations searching for domain homologies (Smart and InterProScan programs) and functional descriptions (KEGG and GO terms) allowed this data to be organized into distinct categories. This data represents the first in-depth proteomics analysis of subcellular compartments in E. histolytica and allows a detailed map of vesicle traffic components in an ancient single-cell organism that lacks a stereotypical ER and Golgi apparatus to be established. The data are related to [1].
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France ; INSERM U786, F-75015 Paris, France ; Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, F-75015 Paris, France.
authors.forename: Doranda
authors.initials: D
authors.lastname: Perdomo
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France ; INSERM U786, F-75015 Paris, France.
authors.forename: Nawel
authors.initials: N
authors.lastname: Aït-Ammar
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France ; INSERM U786, F-75015 Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Syan
authors.affiliation: Institut Pasteur, Imagopole, Plate-Forme de Microscopie Ultrastructurale, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Sachse
authors.affiliation: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Gagan Deep
authors.initials: GD
authors.lastname: Jhingan
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France ; INSERM U786, F-75015 Paris, France.
authors.forename: Nancy
authors.initials: N
authors.lastname: Guillén