title: Bio-inspired mineralization of hydroxyapatite in 3D silk fibroin hydrogel for bone tissue engineering.
abstract: To fabricate hard tissue implants with bone-like structure using a biomimetic mineralization method is drawing much more attentions in bone tissue engineering. The present work focuses in designing 3D silk fibroin hydrogel to modulate the nucleation and growth of hydroxyapatite crystals via a simple ion diffusion method. The study indicates that Ca(2+) incorporation within the hydrogel provides the nucleation sites for hydroxyapatite crystals and subsequently regulates their oriented growth. The mineralization process is regulated in a Ca(2+) concentration- and minerlization time-dependent way. Further, the compressive strength of the mineralized hydrogels is directly proportional with the mineral content in hydrogel. The orchestrated organic/inorganic composite supports well the viability and proliferation of human osteoblast cells; improved cyto-compatibility with increased mineral content. Together, the present investigation reports a simple and biomimetic process to fabricate 3D bone-like biomaterial with desired efficacy to repair bone defects.
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Yashi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India; Institute of Tissue Regeneration Engineering (ITREN) & Department of Nanobiomedical Science BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea.
authors.forename: Banani
authors.initials: B
authors.lastname: Kundu
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address: yaoj@zstu.edu.cn.
authors.forename: Juming
authors.initials: J
authors.lastname: Yao
mesh_terms: D001672:Biocompatible Materials; D001842:Bone and Bones; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D008903:Minerals; D047011:Silk; D005349:Fibroins; D017886:Durapatite
keywords: Biomaterials; Bone tissue engineering; Crystal growth; Hydrogel; Hydroxyapatite; Silk fibroin