title: Extracellular peptidases from Deinococcus radiodurans.
abstract: The extremophile Deinococcus radiodurans wild type R1 produces peptidases (metallo- and serine-) in TGY medium and in the media supplemented with human hair (HMY) and chicken feathers (FMY). Enzymatic screening on agar plates revealed peptidase activity. In TGY medium metallopeptidases were detected corresponding to a molecular mass range of 300-85 kDa (gelatinases); 280-130 (caseinases) and a 300 and a 170 kDa (keratinases); and a gelatinolytic serine peptidase (75 kDa). In HMY medium after 144 h, D. radiodurans produced keratinase (290 U/ml), gelatinase (619 U/ml) and sulfite (26 µg/ml). TGY medium produced higher proteolytic activity: 950 U/ml of gelatinolytic (24 h); 470 U/ml of keratinolytic (24 h) and 110 U/ml of caseinolytic (72 h). In the FMY medium, we found gelatinolytic (317 U/ml), keratinolytic (43 U/ml) and caseinolytic (85 U/ml) activities. The sulfite had a maximum release at 48 h (8.1 µg/ml). Enzymography analysis revealed that the keratinases degraded keratin after 24 h of reaction. The addition of sodium sulfite (1.0 %) improved the keratin degradation. Environmental Scanning Electron microscopy revealed alterations such as damage and holes in the hair fiber cuticle after D. radiodurans growth. This work presents for the first time D. radiodurans as a new keratinolytic microorganism.
authors.affiliation: BIOINOVAR: Unidade de Biocatálise, Bioprodutos e Bioenergia, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
authors.forename: Gabriel Z L
authors.initials: GZ
authors.lastname: Dalmaso
authors.forename: Claudia A S
authors.initials: CA
authors.lastname: Lage
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Mazotto
authors.forename: Edilma Paraguai de Souza
authors.initials: EP
authors.lastname: Dias
authors.forename: Lucio Ayres
authors.initials: LA
authors.lastname: Caldas
authors.forename: Davis
authors.initials: D
authors.lastname: Ferreira
authors.forename: Alane B
authors.initials: AB
authors.lastname: Vermelho
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002364:Caseins / Q000737:chemistry; D034301:Deinococcus / Q000201:enzymology*; D005780:Gelatin / Q000737:chemistry; D007633:Keratins / Q000737:chemistry; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002364:Caseins; D007633:Keratins; D005780:Gelatin; D008666:Metalloendopeptidases