title: Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.
abstract: Transcriptome profiles of the red- and white-fleshed apples in an F1 segregating population of Malus sieversii f.Niedzwetzkyana and M.domestica 'Fuji' were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, United States of America.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China; Shandong Centre of Crop Germ-plasm Resources, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Naibin
authors.initials: N
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Zongying
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Xiaohao
authors.initials: X
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China; Tobacco Laboratory, Shandong Agricultural University, Tai'An, Shandong, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, United States of America.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, United States of America.
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Xuesen
authors.initials: X
authors.lastname: Chen
mesh_terms: D003433:Crosses, Genetic; D005419:Flavonoids / Q000096:biosynthesis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing; D027845:Malus / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D005419:Flavonoids; D020598:Proto-Oncogene Proteins c-myb