title: Single nucleotide polymorphisms of microRNA processing genes and outcome of non-Hodgkin's lymphoma.
abstract: OBJECTIVE
  microRNA (miRNA)-related single nucleotide polymorphisms (miR-SNPs) in miRNA-processing machinery genes can affect cancer risk, treatment efficacy, and patients' prognosis by mediating the expression of targeted genes. Five miR-SNPs in miRNA processing machinery genes, including XPO5 (rs11077), RAN (rs14035), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348), in 168 non-Hodgkin's lymphoma (NHL) patients were evaluated for their association with the cancer risk and outcomes associated with NHL.
  
  
  METHODS
  miR-SNPs were genotyped using polymerase chain reaction-ligase detection reaction. The survival curves were calculated using the Kaplan-Meier method, and comparisons between the curves were made using the log-rank test. Multivariate survival analysis was performed using a Cox proportional hazards model.
  
  
  RESULTS
  Among the five SNPs, only rs197412 located in the coding region of the GEMIN3 gene was identified; it was independently associated with overall survival in NHL patients, as determined by multivariate analysis (relative risk: 1.649; 95% confidence interval: 1.110-2.449; P=0.013). The prognostic value of this miR-SNP in patient outcomes was also observed in the diffuse large B-cell lymphoma and T-cell lymphoma NHL subtypes.
  
  
  CONCLUSIONS
  Our results suggested that the specific genetic variants observed in the miRNA machinery genes may affect NHL survival.
authors.affiliation: Department of Hematology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Hematology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Lanping
authors.initials: L
authors.lastname: Diao
authors.affiliation: Department of Hematology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Guo
keywords: GEMIN3; NHL; miR-SNP; rs197412; survival