title: Generation and Characterization of Novel Magnetic Field-Responsive Biomaterials.
abstract: We report the preparation of novel magnetic field-responsive tissue substitutes based on biocompatible multi-domain magnetic particles dispersed in a fibrin-agarose biopolymer scaffold. We characterized our biomaterials with several experimental techniques. First we analyzed their microstructure and found that it was strongly affected by the presence of magnetic particles, especially when a magnetic field was applied at the start of polymer gelation. In these samples we observed parallel stripes consisting of closely packed fibers, separated by more isotropic net-like spaces. We then studied the viability of oral mucosa fibroblasts in the magnetic scaffolds and found no significant differences compared to positive control samples. Finally, we analyzed the magnetic and mechanical properties of the tissue substitutes. Differences in microstructural patterns of the tissue substitutes correlated with their macroscopic mechanical properties. We also found that the mechanical properties of our magnetic tissue substitutes could be reversibly tuned by noncontact magnetic forces. This unique advantage with respect to other biomaterials could be used to match the mechanical properties of the tissue substitutes to those of potential target tissues in tissue engineering applications.
authors.affiliation: Department of Applied Physics, Faculty of Sciences, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
authors.forename: Modesto T
authors.initials: MT
authors.lastname: Lopez-Lopez
authors.affiliation: Department of Histology, Faculty of Medicine, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scionti
authors.affiliation: Department of Histology, Faculty of Medicine, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Oliveira
authors.affiliation: Department of Applied Physics, Faculty of Sciences, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
authors.forename: Juan D G
authors.initials: JD
authors.lastname: Duran
authors.affiliation: Department of Histology, Faculty of Medicine, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Campos
authors.affiliation: Department of Histology, Faculty of Medicine, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Alaminos
authors.affiliation: Department of Histology, Faculty of Medicine, University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain; Department of Histology, School of Dentistry, National University of Cordoba, Cordoba, Argentina.
authors.forename: Ismael A
authors.initials: IA
authors.lastname: Rodriguez
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D060526:Magnetic Fields; D009061:Mouth Mucosa / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials