title: An Interactive Database for the Assessment of Histone Antibody Specificity.
abstract: Access to high-quality antibodies is a necessity for the study of histones and their posttranslational modifications (PTMs). Here we debut the Histone Antibody Specificity Database (http://www.histoneantibodies.com), an online and expanding resource cataloging the behavior of widely used, commercially available histone antibodies by peptide microarray. This interactive web portal provides a critical resource to the biological research community that routinely uses these antibodies as detection reagents for a wide range of applications.
authors.affiliation: Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA. Electronic address: scott.rothbart@vai.org.
authors.forename: Scott B
authors.initials: SB
authors.lastname: Rothbart
authors.affiliation: Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
authors.forename: Bradley M
authors.initials: BM
authors.lastname: Dickson
authors.affiliation: Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Jesse R
authors.initials: JR
authors.lastname: Raab
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Adrian T
authors.initials: AT
authors.lastname: Grzybowski
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Krajewski
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Angela H
authors.initials: AH
authors.lastname: Guo
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Erin K
authors.initials: EK
authors.lastname: Shanle
authors.affiliation: Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Steven Z
authors.initials: SZ
authors.lastname: Josefowicz
authors.affiliation: Department of Biology, Tufts University, Medford, MA 02155, USA.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Fuchs
authors.affiliation: Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065, USA.
authors.forename: C David
authors.initials: CD
authors.lastname: Allis
authors.affiliation: Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Terry R
authors.initials: TR
authors.lastname: Magnuson
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Ruthenburg
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: brian_strahl@med.unc.edu.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Strahl
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D000918:Antibody Specificity; D030541:Databases, Genetic; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D011499:Protein Processing, Post-Translational
chemical_list: D000906:Antibodies; D006657:Histones