title: Screening of differentially-expressed genes in the muscles of rabbit breeds with expression profile chip.
abstract: The molecular mechanism underlying muscle development in rabbits is not well-understood. In the current study, differentially-expressed genes were scanned using an expression profile chip in New Zealand white rabbits (introduced breed) and Fujian yellow rabbits (local breed), and some of the genes were tested using reverse transcription-polymerase chain reaction. The amplification results were consistent with the microarray data. Fourteen and 13 genes involved in muscle development were identified in the dorsal longissimus and leg muscles, respectively. Myh6, Myh7, Myh7b, Myo5b, Tnnc1, Tpm3, and Acta2 were scanned in the longissimus and leg muscles. Thus, these genes may be involved in muscle fiber formation and muscle development in rabbits. This study provides a theoretical basis for improving meat quality, as well as for the future development and utilization of local meat rabbit breeds.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China.
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China.
authors.forename: X R
authors.initials: XR
authors.lastname: Yan
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Pan
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China xswu@yzu.edu.cn.
authors.forename: X S
authors.initials: XS
authors.lastname: Wu
mesh_terms: D000818:Animals; D001947:Breeding; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D012313:RNA