title: Expression of HIF-1α in Multipotent Mesenchymal Stromal Cells under Hypoxic Conditions.
abstract: According to the data of reverse-transcription-PCR, long-term culturing of multipotent mesenchymal stromal cells from human adipose tissue at 1% O2 (vs. standard 20% O2) led to a decrease in HIF-1α gene expression, while no changes in this parameter were revealed at 5% O2. Analysis of the time course of HIF-1α mRNA level showed that at the early terms of hypoxic exposure, the expression of this gene increased and the duration of this increase depended on the severity of hypoxia.
authors.affiliation: State Scientific Center Institute of Biomedical Problems, Russian Academy of Medical Sciences, Moscow, Russia, pogodina_m@mail.ru.
authors.forename: M V
authors.initials: MV
authors.lastname: Pogodina
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
mesh_terms: D015687:Cell Hypoxia / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger