title: Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model.
abstract: Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.
authors.affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Vishnubalaji
authors.affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia ; Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Atteya
authors.affiliation: Saudi Electronic University, Saudi Arabia.
authors.forename: May
authors.initials: M
authors.lastname: Al-Nbaheen
authors.affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia ; Bone and Joint Research Group, Centre for Human Development Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Science, University of Southampton, Southampton, UK.
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia ; KMEB, Department of Endocrinology, University of Southern Denmark, Odense, Denmark.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Aldahmash
authors.affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
authors.forename: Nehad M
authors.initials: NM
authors.lastname: Alajez