title: Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.
abstract: Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gangdong, Kyung Hee University, 892 Dongnam-ro, Gangdong-gu, Seoul 134-727, Republic of Korea.
authors.forename: Deok-Won
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kang
authors.affiliation: Department of Molecular & Cell Biology, University of California, Berkeley, 142 LSA, No. 3200, Berkeley, CA 94720-3200, USA.
authors.forename: Won Jae
authors.initials: WJ
authors.lastname: Jeong
authors.affiliation: Disease Research Center, Korea Basic Science Institute, Gangwondaehakgil-1, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Lee
authors.affiliation: Disease Research Center, Korea Basic Science Institute, Gangwondaehakgil-1, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Myeong Seon
authors.initials: MS
authors.lastname: Jeong
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Hak Jun
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Disease Research Center, Korea Basic Science Institute, Gangwondaehakgil-1, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Hae-Ryong
authors.initials: HR
authors.lastname: Song
mesh_terms: D019386:Alendronate / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D019386:Alendronate