title: Effect of Se treatment on glucosinolate metabolism and health-promoting compounds in the broccoli sprouts of three cultivars.
abstract: Broccoli sprouts are natural functional foods for cancer prevention because of their high glucosinolate (GSL) content and high selenium (Se) accumulation capacity. The regulation mechanism of Se on GSL metabolism in broccoli sprouts was explored. In particular, the effects of Se treatment (100 μmol/L selenite and selenate) on the Se, sulfur (S), glucosinolate and sulforaphane contents; myrosinase activity and health-promoting compounds (ascorbic acid, anthocyanin, total phenolics and flavonoids) of three, 5 day old, cultivars were investigated. The treatment did not influence the total GSL and ascorbic acid contents; significantly increased the myrosinase activity and sulforaphane, anthocyanin and flavonoids contents; and decreased the total phenolics content. The increase in sulforaphane during early growth can be primarily attributed to the increased myrosinase activity caused by Se treatment. Broccoli sprouts with suitable selenite and selenate concentrations, in the early growth days, could be desirable for improved human health.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Tian
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China. Electronic address: siyipan@aliyun.com.
authors.forename: Siyi
authors.initials: S
authors.lastname: Pan
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D001937:Brassica / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis; D005961:Glucosinolates / Q000378:metabolism*; D006801:Humans; D017879:Isothiocyanates / Q000032:analysis; D010636:Phenols / Q000032:analysis; D064586:Selenic Acid / Q000494:pharmacology*; D020887:Selenious Acid / Q000494:pharmacology*; D012643:Selenium / Q000032:analysis; D013454:Sulfoxides
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D005961:Glucosinolates; D017879:Isothiocyanates; D010636:Phenols; D013454:Sulfoxides; D020887:Selenious Acid; C016766:sulforaphane; D012643:Selenium; D064586:Selenic Acid
keywords: Broccoli sprouts; Glucosinolates; Health-promoting compounds; Myrosinase activity; Se; Sulforaphane