title: Enhanced Neuroprotection of Minimally Invasive Surgery Joint Local Cooling Lavage against ICH-induced Inflammation Injury and Apoptosis in Rats.
abstract: Hypothermia treatment is one of the neuroprotective strategies that improve neurological outcomes effectively after brain damage. Minimally invasive surgery (MIS) has been an important treatment of intracerebral hemorrhage (ICH). Herein, we evaluated the neuroprotective effect and mechanism of MIS joint local cooling lavage (LCL) treatment on ICH via detecting the inflammatory responses, oxidative injury, and neuronal apoptosis around the hematoma cavity in rats. ICH model was established by type IV collagenase caudatum infusion. The rats were treated with MIS 6 h after injection, and then were lavaged by normothermic (37 °C) and hypothermic (33 °C) normal saline in brain separately. The results indicated that MIS joint LCL treatment showed enhanced therapeutic effects against ICH-induced inflammation injury and apoptosis in rats, as convinced by the decline of TUNEL-positive cells, followed by the decrease of IL-1β and LDH and increase of IL-10 and SOD. This study demonstrated that the strategy of using MIS joint LCL may achieve enhanced neuroprotection against ICH-induced inflammation injury and apoptosis in rats with potential clinic application.
authors.affiliation: Department of Neurology, Shandong University School of Medicine, Jinan, 250012, Shandong, China.|Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.|Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, Shandong, China.
authors.forename: Xi-Chang
authors.initials: XC
authors.lastname: Liu
authors.affiliation: Taian Chinese Medicine Hospital, Taian, 271000, Shandong, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Jing
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Yang
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.|Taishan Vocational College of Nursing, Taian, 271000, Shandong, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Shandong Provincial Hospital Affiliated To Shandong University, Jinan, 250012, Shandong, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Basic Medicine, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Fu
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Hou
authors.affiliation: School of Basic Medicine, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Sun
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Zong-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Lei-Lei
authors.initials: LL
authors.lastname: Mao
authors.affiliation: Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, Shandong, China.
authors.forename: You-Mei
authors.initials: YM
authors.lastname: Tang
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Fu
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China. tcdfan66@163.com.
authors.forename: Cun-Dong
authors.initials: CD
authors.lastname: Fan
authors.affiliation: Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China.
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Department of Neurology, Shandong University School of Medicine, Jinan, 250012, Shandong, China. blsun@tsmc.edu.cn.|Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, 271000, Shandong, China. blsun@tsmc.edu.cn.|Affiliated Hospital of Taishan Medical University, Taian, 271000, Shandong, China. blsun@tsmc.edu.cn.
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001929:Brain Edema / Q000601:surgery*; D001930:Brain Injuries / Q000601:surgery; D002543:Cerebral Hemorrhage / Q000150:complications* / Q000628:therapy; D007249:Inflammation / Q000601:surgery; D008297:Male; D019060:Minimally Invasive Surgical Procedures / Q000379:methods; D009474:Neurons / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D007507:Therapeutic Irrigation / Q000379:methods
keywords: Intracerebral hemorrhage; Local cooling lavage; Minimally invasive surgery; Neuronal cell apoptosis