title: Construction of a high efficiency copper adsorption bacterial system via peptide display and its application on copper dye polluted wastewater.
abstract: For the construction of an efficient copper waste treatment system, a cell surface display strategy was employed. The copper adsorption ability of recombinant bacterial strains displaying three different copper binding peptides were evaluated in LB Luria-Bertani medium (LB), artificial wastewater, and copper phthalocyanine containing textile dye industry wastewater samples. Structural characteristics of the three peptides were also analyzed by similarity-based structure modeling. The best binding peptide was chosen for the construction of a dimeric peptide display and the adsorption ability of the monomeric and dimeric peptide displayed strains were compared. The dimeric peptide displayed strain showed superior copper adsorption in all three tested conditions (LB, artificial wastewater, and textile dye industry wastewater). When the strains were exposed to copper phthalocyanine dye polluted wastewater, the dimeric peptide display [543.27 µmol/g DCW dry cell weight (DCW)] showed higher adsorption of copper when compared with the monomeric strains (243.53 µmol/g DCW).
authors.affiliation: School of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Republic of Korea.
authors.forename: Murali Kannan
authors.initials: MK
authors.lastname: Maruthamuthu
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Saravanan Prabhu
authors.initials: SP
authors.lastname: Nadarajan
authors.affiliation: School of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Republic of Korea.
authors.forename: Irisappan
authors.initials: I
authors.lastname: Ganesh
authors.affiliation: Department of Pharmaceutical Science and Technology, Catholic University of Daegu, 330 Geumrak 1-Ri, Hayang-eup, Gyeongsan-Si, Gyeongbuk, 712-702, Republic of Korea.
authors.forename: Sambandam
authors.initials: S
authors.lastname: Ravikumar
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
authors.affiliation: School of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Republic of Korea.
authors.forename: Ik-Keun
authors.initials: IK
authors.lastname: Yoo
authors.affiliation: School of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Republic of Korea. shhong@ulsan.ac.kr.
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000327:Adsorption; D003300:Copper / Q000737:chemistry*; D004926:Escherichia coli; D019151:Peptide Library; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D003300:Copper; D019151:Peptide Library; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Copper binding peptide; Escherichia coli; Peptide display; Phthalocyanin