title: Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells with Platelet-Rich Plasma Accelerate Distraction Osteogenesis in A Canine Model.
abstract: OBJECTIVE
  Distraction osteogenesis (DO) is a surgical procedure used to generate large volumes of new bone for limb lengthening.
  
  
  METHODS
  In this animal experimental study, a 30% lengthening of the left tibia (mean distraction distance: 60.8 mm) was performed in ten adult male dogs by callus distraction after osteotomy and application of an Ilizarov fixator. Distraction was started on postoperative day seven with a distraction rate of 0.5 mm twice per day and carried out at a rate of 1.5 mm per day until the end of the study. Autologous bone marrow mesenchymal stem cells (BM-MSCs) and platelet-rich plasma (PRP) as the treatment group (n=5) or PRP alone (control group, n=5) were injected into the distracted callus at the middle and end of the distraction period. At the end of the consolidation period, the dogs were sacrificed after which computerized tomography (CT) and histomorphometric evaluations were performed.
  
  
  RESULTS
  Radiographic evaluationsrevealed that the amount and quality of callus formations were significantly higher in the treatment group (P<0.05). As measured by CT scan, the healing parametersin dogs of the treatment group were significantly greater (P<0.05). New bone formation in the treatment group was significantly higher (P<0.05).
  
  
  CONCLUSIONS
  The present study showed that the transplantation of BM-MSCs positively affects early bony consolidation in DO. The use of MSCs might allow a shortened period of consolidation and therefore permit earlier device removal.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Dehghan
authors.affiliation: Department of Stem Cells and Developmental Biology at Cell Sciences Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Mohamadreza
authors.initials: M
authors.lastname: Baghaban Eslaminejad
authors.affiliation: Limb Lengthening of Iran, Tehran, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Motallebizadeh
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz University, Tabriz, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Ashrafi Halan
authors.affiliation: Department of Stem Cells and Developmental Biology at Cell Sciences Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Tagiyar
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Sarang
authors.initials: S
authors.lastname: Soroori
authors.affiliation: Department of Stem Cells and Developmental Biology at Cell Sciences Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Agbibi
authors.initials: A
authors.lastname: Nikmahzar
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Mirsepehr
authors.initials: M
authors.lastname: Pedram
authors.affiliation: Department of Stem Cells and Developmental Biology at Cell Sciences Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Shahverdi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Kazemi Mehrjerdi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Sadra
authors.initials: S
authors.lastname: Izadi
keywords: Autologous Transplantation; Bone Lengthening; Distraction Osteogenesis; Mesenchymal Stem Cells; Platelet-Rich Plasma