title: Quantitative Proteomics Reveals the Essential Roles of Stromal Interaction Molecule 1 (STIM1) in the Testicular Cord Formation in Mouse Testis.
abstract: Testicular cord formation in male gonadogenesis involves assembly of several cell types, the precise molecular mechanism is still not well known. With the high-throughput quantitative proteomics technology, a comparative proteomic profile of mouse embryonic male gonads were analyzed at three time points (11.5, 12.5, and 13.5 days post coitum), corresponding to critical stages of testicular cord formation in gonadal development. 4070 proteins were identified, and 338 were differentially expressed, of which the Sertoli cell specific genes were significant enrichment, with mainly increased expression across testis cord development. Additionally, we found overrepresentation of proteins related to oxidative stress in these Sertoli cell specific genes. Of these differentially expressed oxidative stress-associated Sertoli cell specific protein, stromal interaction molecule 1, was found to have discrepant mRNA and protein regulations, with increased protein expression but decreased mRNA levels during testis cord development. Knockdown of Stim1 in Sertoli cells caused extensive defects in gonadal development, including testicular cord disruption, loss of interstitium, and failed angiogenesis, together with increased levels of reactive oxygen species. And suppressing the aberrant elevation of reactive oxygen species could partly rescue the defects of testicular cord development. Taken together, our results suggest that reactive oxygen species regulation in Sertoli cells is important for gonadogenesis, and the quantitative proteomic data could be a rich resource to the elucidation of regulation of testicular cord development.
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Shen
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yueshuai
authors.initials: Y
authors.lastname: Guo
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China guo_xuejiang@njmu.edu.cn zhouzm@njmu.edu.cn.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China guo_xuejiang@njmu.edu.cn zhouzm@njmu.edu.cn.
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: From the ‡State Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism; D008297:Male; D008813:Mice, Inbred ICR; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000071737:Stromal Interaction Molecule 1; D013737:Testis / Q000196:embryology / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C100757:Stim1 protein, mouse; D000071737:Stromal Interaction Molecule 1