title: Overexpression of transforming growth factor type III receptor restores TGF-β1 sensitivity in human tongue squamous cell carcinoma cells.
abstract: The transforming growth factor type III receptor (TβRIII), also known as β-glycan, is a multi-functional sensor that regulates growth, migration and apoptosis in most cancer cells. We hereby investigated the expression of TβRIII in clinical specimens of tongue squamous cell carcinoma (TSCC) and the underlying mechanism that TβRIII inhibits the growth of CAL-27 human oral squamous cells. The TSCC tissues showed a significant decrease in TβRIII protein expression as detected by immunohistochemistry (IHC) and western blot analysis. Transfection of TβRIII-containing plasmid DNA dramatically promoted TGF-β1 (10 ng/ml)-induced decrease in cell viability, apoptosis and cell arrest at the G0-/G1-phase. Moreover, transient overexpression of TβRIII enhanced the TGF-β1-induced cyclin-dependent kinase inhibitor 2b (CDKN2b) and p38 protein activity, but did not affect the activities of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase 1/2 (JNK1/2) in CAL-27 cells. These results suggest overexpression of TβRIII receptor restored TGF-β1 sensitivity in CAL-27 cells, which may provide some new insights on exploiting this molecule therapeutically.
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P. R. China.
authors.forename: Duo
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P. R. China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P. R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin 150081, P. R. China wxf_8888@yahoo.com dongxingli@hotmail.com.
authors.forename: Xingli
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P. R. China wxf_8888@yahoo.com dongxingli@hotmail.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C071736:betaglycan; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: apoptosis; cell cycle; tongue squamous cell carcinoma; transforming growth factor type III receptor (TβRIII); transforming growth factor-β1 (TGF-β1)