title: Antioxidant effects of gastrointestinal digested purple carrot extract on the human cells of colonic mucosa.
abstract: Purple carrot (PC) is a potential dietary constituent, which represents a valuable source of antioxidants and can modulate the reactive oxygen species (ROS) level in the gastrointestinal tract. Antioxidant capacity of a PC extract subjected to digestion process simulated in the artificial alimentary tract, including the stomach, small intestine and colon, was analyzed in normal human cells of colon mucosa. Results indicated that the extract obtained upon passage through the gastrointestinal tract, which could come into contact with the colonic cells in situ, was less potent than the extract, which was not subjected to digestion process. Digested PC extract exhibited intracellular ROS-inhibitory capacity, with 1mg/mL showing the ROS clearance of 18.4%. A 20.7% reduction in oxidative DNA damage due to colon mucosa cells' treatment with digested PC extract was observed. These findings indicate that PC extract is capable of colonic cells' protection against the adverse effects of oxidative stress.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland. Electronic address: aolejnik@up.poznan.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Olejnik
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rychlik
authors.affiliation: Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, Poznan 60-624, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kidoń
authors.affiliation: Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, Poznan 60-624, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Czapski
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kowalska
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Juzwa
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Olkowicz
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Dembczyński
authors.affiliation: INCELL Corporation, LLC, San Antonio, TX 78249, United States.
authors.forename: Mary Pat
authors.initials: MP
authors.lastname: Moyer
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage; D018552:Daucus carota / Q000737:chemistry*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species
keywords: Anthocyanins; Antioxidant capacity; Colon mucosa; DNA damage; In vitro digestion; Intracellular ROS; Oxidative stress; Purple carrot