title: Data in support of protocol for rat single muscle-fiber isolation and culture.
abstract: This data article contains data related to the research article entitled, "Protocol for rat single muscle-fiber isolation and culture" by Komiya et al. [1]. It has yet to be shown whether adult myosin heavy chain (MyHC) isoforms are expressed at a readily detectable level in cultured myotubes. In this study, we examined whether adult MyHC isoforms could be detected in myotubes differentiated from rat satellite cells using a Western blotting assay and specific antibodies against slow MyHC, fast MyHC and pan-MyHC. Results showed that slow adult MyHC isoforms were faintly detected in myotubes, suggesting that rat myotubes express adult MyHC isoforms although that amount is very low.
authors.affiliation: Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Komiya
authors.affiliation: Department of Biological Sciences, University of Manitoba, Winnipeg, Canada R3T 2N2.
authors.forename: Judy E
authors.initials: JE
authors.lastname: Anderson
authors.affiliation: Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Akahoshi
authors.affiliation: Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Mako
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Tatsumi
authors.affiliation: Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Ikeuchi
authors.affiliation: Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Mizunoya
keywords: Muscle fiber type; Myotube; Primary culture; Skeletal muscle; Western blotting