title: Inhibitory kinetics and mechanism of kaempferol on α-glucosidase.
abstract: α-Glucosidase is a therapeutic target for diabetes mellitus, and α-glucosidase inhibitors play a vital role in the treatments for the disease. As a kind of potentially safer α-glucosidase inhibitor, flavonoids have attached much attention currently. In this study, kaempferol was found to show a notable inhibition activity on α-glucosidase in a mixed-type manner with IC50 value of (1.16 ± 0.04) × 10(-5) mol L(-1). Analyses of fluorescence, circular dichroism and Fourier transform infrared spectra indicated that kaempferol bound to α-glucosidase with high affinity which was mainly driven by hydrogen bonds and van der Waals forces, and this binding resulted in conformational alteration of α-glucosidase. Further molecular docking study validated the experimental results. It was proposed that kaempferol may interact with some amino acid residues located within the active site of α-glucosidase, occupying the catalytic center of the enzyme to avoid the entrance of p-nitrophenyl-α-D-glucopyranoside and ultimately inhibiting the enzyme activity.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: gwzhang@ncu.edu.cn.
authors.forename: Guowen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Liao
authors.affiliation: School of Biological Sciences, The University of Auckland, Auckland 1142, New Zealand.
authors.forename: Deming
authors.initials: D
authors.lastname: Gong
mesh_terms: D005419:Flavonoids / Q000737:chemistry; D044949:Kaempferols / Q000737:chemistry*; D007700:Kinetics; D062105:Molecular Docking Simulation / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005419:Flavonoids; D044949:Kaempferols; C006552:kaempferol; D000520:alpha-Glucosidases
keywords: Inhibition mechanism; Inhibitory kinetics; Kaempferol; Molecular docking; Spectroscopy; α-Glucosidase