title: Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts.
abstract: In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12mg phloroglucinol equivalents g(-1), respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC×RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range.
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: Montero
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Andrea P
authors.initials: AP
authors.lastname: Sánchez-Camargo
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: García-Cañas
authors.affiliation: LEMAR UMR 6539 CNRS UBO IRD IFREMER, Université de Bretagne Occidentale (UBO), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, Rue Dumont d'Urville, Plouzané 29280, France.
authors.forename: Anaëlle
authors.initials: A
authors.lastname: Tanniou
authors.affiliation: LEMAR UMR 6539 CNRS UBO IRD IFREMER, Université de Bretagne Occidentale (UBO), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, Rue Dumont d'Urville, Plouzané 29280, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Stiger-Pouvreau
authors.affiliation: Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, loc. Feo di Vito, 89122 Reggio Calabria, Italy.
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Russo
authors.affiliation: Dipartimento di Farmacia, Università di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Rastrelli
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain. Electronic address: m.herrero@csic.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Herrero
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Ibáñez
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001267:Atlantic Ocean; D049109:Cell Proliferation / Q000187:drug effects; D002853:Chromatography, Liquid; D019073:HT29 Cells; D006801:Humans; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010696:Phloroglucinol / Q000737:chemistry / Q000494:pharmacology; D044647:Sargassum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D013634:Tannins / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010636:Phenols; D013634:Tannins; D010696:Phloroglucinol
keywords: Anti-proliferative activity; Brown algae; Comprehensive LC; Phenolic compounds; Phlorotannins; Sargassum muticum