title: Laccase produced by a thermotolerant strain of Trametes trogii LK13.
abstract: Thermophilic and thermotolerant micro-organisms strains have served as the natural source of industrially relevant and thermostable enzymes. Although some strains of the Trametes genus are thermotolerant, few Trametes strains were studied at the temperature above 30 °C until now. In this paper, the laccase activity and the mycelial growth rate for Trametes trogii LK13 are superior at 37 °C. Thermostability and organic cosolvent tolerance assays of the laccase produced at 37 °C indicated that the enzyme possessed fair thermostability with 50% of its initial activity at 80 °C for 5 min, and could remain 50% enzyme activity treated with organic cosolvent at the concentration range of 25%-50% (v/v). Furthermore, the test on production of laccase and lignocellulolytic enzymes showed the crude enzymes possessed high laccase level (1000 U g (-1) ) along with low cellulose (2 U g (-1) ) and xylanase (140 U g (-1) ) activity. Thus, T. trogii LK13 is a potential strain to be applied in many biotechnological processes.
authors.affiliation: Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Kunming Yunnan, PR China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Yan
authors.affiliation: College of Life Science, The Southwest Forest University, Kunming Yunnan, PR China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Kunming Yunnan, PR China.
authors.forename: Jiezhen
authors.initials: J
authors.lastname: Niu
authors.affiliation: Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Kunming Yunnan, PR China.
authors.forename: Daidi
authors.initials: D
authors.lastname: Chen
authors.affiliation: Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Kunming Yunnan, PR China.
authors.forename: Irbis
authors.initials: I
authors.lastname: Chagan
mesh_terms: D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012997:Solvents; D013696:Temperature; D055454:Trametes / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development* / Q000528:radiation effects
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012997:Solvents; D042845:Laccase
keywords: Laccase; Trametes trogii; organic cosolvent tolerance; thermostability; thermotolerant fungi