title: Macroscopic modeling of mammalian cell growth and metabolism.
abstract: We review major modeling strategies and methods to understand and simulate the macroscopic behavior of mammalian cells. These strategies comprise two important steps: the first step is to identify stoichiometric relationships for the cultured cells connecting the extracellular inputs and outputs. In a second step, macroscopic kinetic models are introduced. These relationships together with bioreactor and metabolite balances provide a complete description of a system in the form of a set of differential equations. These can be used for the simulation of cell culture performance and further for optimization of production.
authors.affiliation: Biochemical Engineering Institute, Saarland University, Campus A1.5, D-66123, Saarbruecken, Germany.
authors.forename: Bassem
authors.initials: B
authors.lastname: Ben Yahia
authors.forename: Laetitia
authors.initials: L
authors.lastname: Malphettes
authors.forename: Elmar
authors.initials: E
authors.lastname: Heinzle
mesh_terms: D000818:Animals; D019149:Bioreactors; D002460:Cell Line; D049109:Cell Proliferation; D004734:Energy Metabolism; D008322:Mammals; D008954:Models, Biological