title: Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells.
abstract: We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.
authors.forename: Eddie Y
authors.initials: EY
authors.lastname: Chung
authors.affiliation: ‡Singapore-MIT Alliance for Research and Technology, BioSystems and Micromechanics, Singapore 138602.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Ochs
authors.affiliation: §Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: §Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.affiliation: §Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Smith
authors.affiliation: ∥Sanford Burnham Medical Research Institute, La Jolla, California 92037, United States.
authors.forename: Alex Y
authors.initials: AY
authors.lastname: Strongin
authors.affiliation: ‡Singapore-MIT Alliance for Research and Technology, BioSystems and Micromechanics, Singapore 138602.
authors.forename: Roger
authors.initials: R
authors.lastname: Kamm
authors.affiliation: ⊥Institute of Mechanobiology and Medical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ying-Xin
authors.initials: YX
authors.lastname: Qi
authors.affiliation: §Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Lu
authors.affiliation: §Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois 61801, United States.
authors.forename: Yingxiao
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques / Q000379:methods; D045744:Cell Line, Tumor; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000032:analysis / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000201:enzymology*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D045663:Quantum Dots / Q000737:chemistry / Q000378:metabolism; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D053511:Matrix Metalloproteinase 14
keywords: FRET nanosensor; MT1-MMP; activatable cell-penetrating peptide; cancer; multiplex signals; single cell