title: Schisandrin B inhibits the proliferation of human lung adenocarcinoma A549 cells by inducing cycle arrest and apoptosis.
abstract: Lung cancer is the leading cause of cancer death in the world. Schizandrin B (Sch B) is one of the main dibenzocyclooctadiene lignans present in the fruit of Schisandra chinensis (Schisandraceae). Sch B has multiple functions against cancer. The aim of this study was to determine the effect of Sch B on the proliferation, cell cycling, apoptosis and invasion of lung adenocarcinoma A549 cells by MTT, flow cytometry, wound healing and transwell invasion assays. Treatment with Sch B inhibited the proliferation of A549 cells in a dose-dependent manner. Sch B induced cell cycle arrest at G0/G1 phase by down-regulating the expression of cyclin D1, cyclin-dependent kinase (CDK)4, and CDK6, but up-regulating p53 and p21 expression in A549 cells. Furthermore, Sch B triggered A549 cell apoptosis by increasing Bax, cleaved caspase-3, 9, Cyto C, but decreasing Bcl-2 and PCNA expression. In addition, Sch B inhibited the invasion and migration of A549 cells by down-regulating the expressions of HIF-1, VEGF, MMP-9 and MMP-2. Therefore, Sch B has potent anti-tumor activity and may be a promising traditional Chinese medicine for human lung carcinoma.
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
authors.forename: Xue-Jiao
authors.initials: XJ
authors.lastname: Lv
authors.affiliation: Department of Pathophysiology, College of Basic Medicine, Jilin University Changchun 130021, Jilin, China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
authors.forename: Yu-Qiu
authors.initials: YQ
authors.lastname: Hao
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Su
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
authors.forename: Jun-Yao
authors.initials: JY
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, College of Basic Medicine, Jilin University Changchun 130021, Jilin, China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Du
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
keywords: A549 cells; Schizandrin B; cell cycling; invasion; migration; proliferation