title: Bioactivity characterization of Lactobacillus strains isolated from dairy products.
abstract: This study aimed to find candidate strains of Lactobacillus isolated from sheep dairy products (yogurt and ewe colostrum) with probiotic and anticancer activity. A total of 100 samples were randomly collected from yogurt and colostrum and 125 lactic acid bacteria were isolated. Of these, 17 Lactobacillus strains belonging to five species (L. delbrueckii, L. plantarum, L. rhamnosus, L. paracasei, and L. casei) were identified. L. plantarum 17C and 13C, which isolated from colostrums, demonstrated remarkable results such as resistant to low pH and high concentrations of bile salts, susceptible to some antibiotics and good antimicrobial activity that candidate them as potential probiotics. Seven strains (1C, 5C, 12C, 13C, 17C, 7M, and 40M), the most resistant to simulated digestion, were further investigated to evaluate their capability to adhere to human intestinal Caco-2 cells. L. plantarum 17C was the most adherent strain. The bioactivity assessment of L. plantarum 17C showed anticancer effects via the induction of apoptosis on HT-29 human cancer cells and negligible side effects on one human epithelial normal cell line (FHs 74). The metabolites produced by this strain can be used as alternative pharmaceutical compounds with promising therapeutic indices because they are not cytotoxic to normal mammalian cells.
authors.affiliation: Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Babak
authors.initials: B
authors.lastname: Haghshenas
authors.affiliation: Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Nami
authors.affiliation: School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Minoo
authors.initials: M
authors.lastname: Haghshenas
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Norhafizah
authors.initials: N
authors.lastname: Abdullah
authors.affiliation: Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Dayang
authors.initials: D
authors.lastname: Radiah
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.|Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ahmad Yari
authors.initials: AY
authors.lastname: Khosroushahi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis; D001422:Bacterial Adhesion; D001647:Bile Acids and Salts / Q000633:toxicity; D002460:Cell Line; D003126:Colostrum / Q000382:microbiology; D003611:Dairy Products / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D019936:Probiotics / Q000302:isolation & purification* / Q000494:pharmacology*; D012756:Sheep
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D001647:Bile Acids and Salts
keywords: Colostrum; Lactobacillus; molecular method; probiotic