title: An Improved Method With High Specificity for KIR2DL1 Functional Allele Typing.
abstract: As new killer-cell immunoglobulin-like receptor (KIR) alleles are discovered, a challenge in KIR typing is maintaining sensitivity and specificity. A single nucleotide polymorphism assay can be used to type functional KIR2DL1 alleles. We improved recently on the earlier method by using a higher-specificity assay. The major modifications include the development of sequence-specific primers to selectively amplify the transmembrane domain of all known KIR2DL1 alleles via polymerase chain reaction with sequence-specific primers (PCR-SSP), and using the PCR products as the template for a revised KIR2DL1 functional allele-typing assay. This modified method allows high-throughput typing with high specificity.
authors.affiliation: Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, Memphis, Tennessee.
authors.forename: Rafijul
authors.initials: R
authors.lastname: Bari
authors.affiliation: Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, Memphis, Tennessee.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schell
authors.affiliation: Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, Memphis, Tennessee.
authors.forename: MaCal
authors.initials: M
authors.lastname: Tuggle
authors.affiliation: Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, Memphis, Tennessee Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee wing.leung@stjude.org.
authors.forename: Wing
authors.initials: W
authors.lastname: Leung
mesh_terms: D000483:Alleles; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D054340:Receptors, KIR / Q000235:genetics / Q000378:metabolism; D054342:Receptors, KIR2DL1 / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: C093100:KIR2DL1 protein, human; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1
keywords: KIR polymorphism; KIR2DL1 allelotyping; KIRs genotyping; killer-cell immunoglobulin-like receptor (KIR); natural killer (NK) cell; single nucleotide polymorphism (SNP) assay