title: L-Asparaginase from Streptomyces griseus NIOT-VKMA29: optimization of process variables using factorial designs and molecular characterization of L-asparaginase gene.
abstract: Marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. In this study, a potential extracellular L-asparaginase was characterised from the Streptomyces griseus NIOT-VKMA29. Box-Behnken based optimization was used to determine the culture medium components to enhance the L-asparaginase production. pH, starch, yeast extract and L-asparagine has a direct correlation for enzyme production with a maximum yield of 56.78 IU mL(-1). A verification experiment was performed to validate the experiment and more than 99% validity was established. L-Asparaginase biosynthesis gene (ansA) from Streptomyces griseus NIOT-VKMA29 was heterologously expressed in Escherichia coli M15 and the enzyme production was increased threefold (123 IU mL(-1)) over the native strain. The ansA gene sequences reported in this study encloses several base substitutions with that of reported sequences in GenBank, resulting in altered amino acid sequences of the translated protein.
authors.affiliation: Andaman and Nicobar Centre for Ocean Science and Technology, Earth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT), Port Blair-744103, Andaman and Nicobar Islands, India.
authors.forename: Balakrishnan
authors.initials: B
authors.lastname: Meena
authors.affiliation: Andaman and Nicobar Centre for Ocean Science and Technology, Earth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT), Port Blair-744103, Andaman and Nicobar Islands, India.
authors.forename: Lawrance
authors.initials: L
authors.lastname: Anburajan
authors.affiliation: Andaman and Nicobar Centre for Ocean Science and Technology, Earth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT), Port Blair-744103, Andaman and Nicobar Islands, India.
authors.forename: Thadikamala
authors.initials: T
authors.lastname: Sathish
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO-NIOT, Ministry of Earth Sciences, Government of India, Chennai-600100, India.
authors.forename: Rangamaran
authors.initials: R
authors.lastname: Vijaya Raghavan
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO-NIOT, Ministry of Earth Sciences, Government of India, Chennai-600100, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Dharani
authors.affiliation: Andaman and Nicobar Centre for Ocean Science and Technology, Earth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT), Port Blair-744103, Andaman and Nicobar Islands, India.
authors.forename: Nambali Valsalan
authors.initials: NV
authors.lastname: Vinithkumar
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO-NIOT, Ministry of Earth Sciences, Government of India, Chennai-600100, India.
authors.forename: Ramalingam
authors.initials: R
authors.lastname: Kirubagaran
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D001215:Asparaginase