title: Human platelet antigen genotyping of platelet donors in southern Brazil.
abstract: Human platelet antigens (HPA) are immunogenic structures that result from single nucleotide polymorphisms (SNPs) leading to single amino acid substitutions. This study sought to determine the allele and genotype frequencies of HPA-1, HPA-2, HPA-3, HPA-4, HPA-5 and HPA-15 in platelet donors from the state of Rio Grande do Sul (RS), Brazil, and compare their allele frequencies to those observed in other populations. HPA genotyping was performed by PCR-SSP method. The study sample comprised 201 platelet donors (167 Caucasians and 34 non-Caucasians). Allele 'a' was that most commonly found for HPA-1 to 5 in both groups. The HPA-15ab genotype predominated over homozygous genotypes of this system. Fisher's exact test revealed statistically significant differences for the HPA-5 system, with a greater prevalence of the HPA-5b allele in non-Caucasians. The neighbour-joining method and principal components analysis revealed genetic proximity between our Caucasian group and European populations. We conclude that the allele frequencies of HPA-1 to 5 and HPA-15 found in our Caucasian sample are similar to those reported for European populations. These findings corroborate the ethnic makeup of the population of RS. The higher frequency of the HPA-5b allele found in the non-Caucasian group of our sample suggests the possibility of allosensitization in patients who receive platelet transfusions from genetically incompatible donors.
authors.affiliation: PPG em Medicina - Ciências Cirúrgicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.|Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: J
authors.identifier: http://orcid.org/0000-0001-9779-0317
authors.initials: J
authors.lastname: Merzoni
authors.affiliation: Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: I S
authors.initials: IS
authors.lastname: Fagundes
authors.affiliation: PPG em Medicina - Ciências Cirúrgicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: L W
authors.initials: LW
authors.lastname: Lunardi
authors.affiliation: Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: J D-R
authors.initials: JD
authors.lastname: Lindenau
authors.affiliation: Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: B C
authors.initials: BC
authors.lastname: Gil
authors.affiliation: Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Jobim
authors.affiliation: Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: V G
authors.initials: VG
authors.lastname: Dias
authors.affiliation: Faculdade de Medicina da UCS, Universidade de Caxias do Sul, Caxias do Sul, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Merzoni
authors.affiliation: Serviço de Hemoterapia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Sekine
authors.affiliation: Serviço de Hemoterapia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: T G H
authors.initials: TG
authors.lastname: Onsten
authors.affiliation: PPG em Medicina - Ciências Cirúrgicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.|Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Jobim
mesh_terms: D000328:Adult; D000941:Antigens / Q000235:genetics*; D001782:Blood Donors; D001792:Blood Platelets / Q000378:metabolism*; D001938:Brazil; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D010802:Phylogeny; D025341:Principal Component Analysis
chemical_list: D000941:Antigens