title: ANTIMICROBIAL ACTIVITY OF EXTRACTS FROM ECUADORIAN LICHENS.
abstract: Antimicrobial activity of the ethanolic, isopropanolic, acetone, DMSO and aqueous extracts of the two lichen species from Ecuadorian highland, Usnea sp. and Stereocaulon sp. were explored in vitro against bacteria Bacillus subtilis, Escherichia coli and Staphylococcus aureus by the disc-diffusion method. Also the minimal inhibitory concentration (MIC) was determined. The strongest antimicrobial activity was found in DMSO extract of Usnea sp. compared to antibacterial activity of ciprfloxacin and cefazolin antibiotics. The inhibition zone was 28 mm, 30 mm, 31mm (DMSO extract, ciprfloxacin and cefazolin respectively) in case of B. subtilis usage as the test bacteria. MIC value for Usnea sp. and Stereocaulon sp. DMSO extracts was 0.4 mg/ml. E. coli was resistant to all kinds of extracts. The S. aureus sensitivity to lichen DMSO extracts was comparable to sensitivity of these microorganisms to tetracycline and vancomycin. Thereby, most kinds of extracts (ethanol, isopropanol, hexane, DMSO and acetone solvents) from Ecuadorian lichens Usnea sp. and Stereocaulon sp. with the exception of aqueous Stereocaulon sp. extracts possessed antibacterial activity against B. subtilis. DMSO lichen extracts had also antimicrobial activity against S. aureus. At the same time the extracts studied didn't demonstrate antibacterial activity against the representatives of the most common and harmful phytopathogenic bacteria tested. Further investigations of Ecuadorian lichens especially study of plants collected from extremal highland biotops can be very important in study of possibility of treatment of numerous diseases caused by pathogenic microorganisms.
authors.forename: N A
authors.initials: NA
authors.lastname: Matvieieva
authors.forename: L A
authors.initials: LA
authors.lastname: Pasichnyk
authors.forename: N V
authors.initials: NV
authors.lastname: Zhytkevych
authors.forename: Pabón Garcés Galo
authors.initials: PG
authors.lastname: Jacinto
authors.forename: V S
authors.initials: VS
authors.lastname: Pidgorskyi
mesh_terms: D019840:2-Propanol; D000096:Acetone; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development; D002437:Cefazolin / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D004484:Ecuador; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D000431:Ethanol; D008011:Lichens / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D012997:Solvents; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D014867:Water
chemical_list: D000900:Anti-Bacterial Agents; D045424:Complex Mixtures; D012997:Solvents; D014867:Water; D000096:Acetone; D000431:Ethanol; D002939:Ciprofloxacin; D002437:Cefazolin; D019840:2-Propanol; D004121:Dimethyl Sulfoxide