title: Quantitative Detection of ID4 Gene Aberrant Methylation in the Differentiation of Myelodysplastic Syndrome from Aplastic Anemia.
abstract: BACKGROUND
  The diagnosis of myelodysplastic syndrome (MDS), especially hypoplastic MDS, and MDS with low blast counts or normal karyotype may be problematic. This study characterized ID4 gene methylation in patients with MDS and aplastic anemia (AA).
  
  
  METHODS
  The methylation status of ID4 was analyzed by bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time methylation-specific PCR (MethyLight PCR) in 100 patients with MDS and 31 patients with AA.
  
  
  RESULTS
  The MDS group had a higher ID4 gene methylation positivity rate (22.22%) and higher methylation levels (0.21 [0-3.79]) than the AA group (P < 0.05). Furthermore, there were significant differences between the hypoplastic MDS and AA groups, the MDS with low blast count and the AA groups, and the MDS with normal karyotype and the AA groups. The combination of genetic and epigenetic markers was used in much more patients with MDS (62.5% [35/56]) than the use of genetic markers only (51.79% [29/56]).
  
  
  CONCLUSIONS
  These results showed that the detection of ID4 methylation positivity rates and levels could be a useful biomarker for MDS diagnosis.
authors.forename: Mian-Yang
authors.initials: MY
authors.lastname: Li
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xu
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Kang
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Cen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000741:Anemia, Aplastic / Q000235:genetics*; D002648:Child; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D005260:Female; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D055815:Young Adult
chemical_list: C490128:ID4 protein, human; D051796:Inhibitor of Differentiation Proteins