title: Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
abstract: We analyzed the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of smudge plate growth for bacterial identification from 400 blood cultures. Ninety-seven percent of Gram-negative bacilli and 85% of Gram-positive organisms were correctly identified within 4 h; only eight isolates (2.0%) were misidentified. This method provided rapid and accurate microbial identification from positive blood cultures.
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Porter
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada Department of Microbiology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
authors.forename: Samira
authors.initials: S
authors.lastname: Mubareka
authors.affiliation: Department of Microbiology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
authors.forename: Leona
authors.initials: L
authors.lastname: Kotowich
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada Department of Microbiology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada andrew.simor@sunnybrook.ca.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Simor
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors