title: Identification of a multi-reassortant G12P[9] rotavirus with novel VP1, VP2, VP3 and NSP2 genotypes in a child with acute gastroenteritis.
abstract: The G12 rotavirus genotype is globally emerging to cause severe gastroenteritis in children. Common G12 rotaviruses have either a Wa-like or DS-1-like genome constellation, while some G12 strains may have unusual genome composition. In this study, we determined the full-genome sequence of a G12P[9] strain (ME848/12) detected in a child hospitalized with acute gastroenteritis in Italy in 2012. Strain ME848/12 showed a complex genetic constellation (G12-P[9]-I17-R12-C12-M11-A12-N12-T7-E6-H2), likely derived from multiple reassortment events, with the VP1, VP2, VP3 and NSP2 genes being established as novel genotypes R12, C12, M11 and N12, respectively. Gathering sequence data on human and animal rotaviruses is important to trace the complex evolutionary history of atypical RVAs.
authors.affiliation: Department of Health Promotion Sciences and Mother and Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy. Electronic address: simona.degrazia@unipa.it.
authors.forename: Simona
authors.initials: S
authors.lastname: De Grazia
authors.affiliation: Department of Health Promotion Sciences and Mother and Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy.
authors.forename: Giovanni M
authors.initials: GM
authors.lastname: Giammanco
authors.affiliation: Veterinary Medical Research Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Renáta
authors.initials: R
authors.lastname: Dóró
authors.affiliation: Department of Health Promotion Sciences and Mother and Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy.
authors.forename: Floriana
authors.initials: F
authors.lastname: Bonura
authors.affiliation: Veterinary Medical Research Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Marton
authors.affiliation: Department of Human Pathology, University of Messina, Messina, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cascio
authors.affiliation: Department of Veterinary Medicine, University Aldo Moro of Bari, Valenzano, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.affiliation: Veterinary Medical Research Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
mesh_terms: D002675:Child, Preschool; D005759:Gastroenteritis / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics*; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D017423:Sequence Analysis, RNA; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D015678:Viral Structural Proteins
keywords: Full genome sequencing; G12P[9]; Genotyping; Human rotavirus; Interspecies transmission; Reassortment