title: Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals.
abstract: The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110, grown in medium supplemented with an essential metal (Cu(2+)) or a non-essential metal (Cd(2+)),were compared using iTRAQ technology. The data were obtained within a larger study that evaluated the overall effects of different heavy metals on growth/survival, EPS production and ultrastructure of this cyanobacterium [1]. To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece's proteomes were evaluated after chronic and acute exposure to Cu(2+) and Cd(2+) in two independent 8-plex iTRAQ studies. For the chronic exposure 0.1 mg/l of Cu(2+) or 5 mg/l of Cd(2+) were used for 10 and 20 days, while in the acute experiments the cells were exposed to 10× these concentrations for 24 h. 202 and 268 proteins were identified and quantified for studies 1 (Cu(2+)) and 2 (Cd(2+)), respectively. The majority of the proteins with significant fold changes were associated with photosynthesis, CO2 fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolism.
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal ; Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Porto, Portugal.
authors.forename: Rita
authors.initials: R
authors.lastname: Mota
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
authors.forename: Sara B
authors.initials: SB
authors.lastname: Pereira
authors.affiliation: Department of Agrifood Production and Environmental Sciences, University of Florence, Florence, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Meazzini
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Fernandes
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal ; Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Porto, Portugal.
authors.forename: Arlete
authors.initials: A
authors.lastname: Santos
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Evans
authors.affiliation: Department of Agrifood Production and Environmental Sciences, University of Florence, Florence, Italy ; Institute of Ecosystem Study (ISE), National Research Council (CNR), Sesto Fiorentino (FI), Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: De Philippis
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Wright
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal ; Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Porto, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Tamagnini
keywords: Cadmium; Copper; Cyanobacteria; Cyanothece; Proteome; iTRAQ