title: Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury.
abstract: OBJECTIVE
  To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells.
  
  
  METHODS
  To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin.
  
  
  RESULTS
  On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells.
  
  
  CONCLUSIONS
  The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury.
authors.affiliation: Department of Analytical Pharmacology, Meijo University Faculty of Pharmacy, Nagoya, Japan ; Department of Nephrology, School of Medicine, Fujita Health University, Toyoake, Japan ; Department of Nephrology, Nagoya University School of Medicine, Nagoya, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Mizuno
authors.affiliation: Department of Nephrology, School of Medicine, Fujita Health University, Toyoake, Japan ; Department of Nephrology, Nagoya University School of Medicine, Nagoya, Japan.
authors.forename: Waichi
authors.initials: W
authors.lastname: Sato
authors.affiliation: Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: Department of Analytical Pharmacology, Meijo University Faculty of Pharmacy, Nagoya, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Terao
authors.affiliation: Department of Nephrology, School of Medicine, Fujita Health University, Toyoake, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Division of Clinical Sciences and Neuropsychopharmacology, Meijo University Faculty of Pharmacy, Nagoya, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Noda
authors.affiliation: Department of Nephrology, School of Medicine, Fujita Health University, Toyoake, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yuzawa
authors.affiliation: Department of Analytical Pharmacology, Meijo University Faculty of Pharmacy, Nagoya, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nagamatsu
keywords: SLC47A1; acute kidney injury; advanced glycation endproducts; cisplatin; diabetes mellitus