title: 131I-Zn-Chlorophyll derivative photosensitizer for tumor imaging and photodynamic therapy.
abstract: In recent years, the photodynamic therapy studies have gained considerable attention as an alternative method to surgery, chemotherapy and radiotherapy which is commonly used to fight cancer. In this study, biological potentials of a benzyloxy bearing zinc(II) pheophorbide-a (Zn-PH-A) were investigated via in vivo and in vitro experiments. Zn-PH-A was labeled with (131)I with high efficiency (95.3 ± 2.7%) and its biodistribution studies were investigated on female Albino Wistar rats. The radiolabeled photosensitizer had been intravenously injected into the tail vein, and then the animals were sacrificed at 30, 60 and 120 min post injection. The percent of radioactivity per gram of organs (%ID/g) was determined. The radiolabeled Zn-PH-A showed high uptake in ovary. In addition, photodynamic therapy studies of the photosensitizer were conducted in EMT6, murine mammary carcinoma and HeLa, human cervix carcinoma cell lines. For the photodynamic therapy studies, the cells with Zn-PH-A were exposed to red light (650 nm) at the doses of 10-30 J/cm(2). The results showed that Zn-PH-A has stronger PDT effect in EMT6 than HeLa cell. Our present work demonstrates (131)I-labeled photosensitizer as a bifunctional agent (PDT and nuclear imaging) which could be improved in future by using EMT6 growing tumor in nude mice.
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343 Yenisehir, Mersin, Turkey; Department of Energy Systems Engineering, Faculty of Technology, Mersin University, TR-33480 Tarsus, Mersin, Turkey. Electronic address: kasim.ocakoglu@mersin.edu.tr.
authors.forename: Kasim
authors.initials: K
authors.lastname: Ocakoglu
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Er
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343 Yenisehir, Mersin, Turkey.
authors.forename: Guven
authors.initials: G
authors.lastname: Kiyak
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey.
authors.forename: Fatma Yurt
authors.initials: FY
authors.lastname: Lambrecht
authors.affiliation: Department of Medical biology, Faculty of Medicine, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gunduz
authors.affiliation: Department of Medical biology, Faculty of Medicine, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Cagla
authors.initials: C
authors.lastname: Kayabasi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D051379:Mice; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D007457:Iodine Radioisotopes; D017319:Photosensitizing Agents; D002734:Chlorophyll; C032623:pheophorbide a; D015032:Zinc
keywords: (131)I; Biodistribution; Cancer; Chlorophyll; Photodynamic therapy; Radiolabeling