title: GPRC5A overexpression predicted advanced biological behaviors and poor prognosis in patients with gastric cancer.
abstract: G protein-coupled receptor, family C, group 5, member A (GPRC5A) had received attentions for its role in carcinogenesis and prognostic values in several types of cancer. However, the functional roles of GPRC5A in gastric cancer (GC) had never been elucidated. The expression levels of GPRC5A were detected by real-time quantitative reverse transcription PCR and Western blot in GC tissues and adjacent non-tumor tissues. GPRC5A expression in tissue sections of 106 GC samples was evaluated using immunohistochemistry. The staining results were compared with clinicopathological factors and to the prognosis of GC patients. The mRNA and protein expression levels of GPRC5A in gastric cancer tissues were higher than those in adjacent non-tumor tissues. Positive GPRC5A expression was significantly correlated with larger size of primary tumor, diffuse type (Lauren's classification), deeper serosal invasion, and more lymph node metastasis. In addition, Kaplan-Meier curve analysis demonstrated that GC patients with positive GPRC5A expression had poor prognosis than those with negative GPRC5A expression. GPRC5A expression was identified as an independent factor of the overall survival in GC patients by multivariate Cox analysis. Further, the overall survival difference existed between patients with GPRC5A positive and negative groups in GC patients with lymph node metastasis. Our results suggested that elevated levels of GPRC5A played significant roles in GC progression. GPRC5A could serve as a prognostic biomarker of GC.
authors.affiliation: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Honggen
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Tianjin Nankai Hospital, Tianjin, 300193, China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Hao
authors.affiliation: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Fanming
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. jyjyfyzlk@126.com.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C116760:GPRC5A protein, human; D043562:Receptors, G-Protein-Coupled
keywords: GPRC5A; Gastric cancer; Immunohistochemistry; Prognosis