title: Quantification of prominent volatile compounds responsible for muskmelon and watermelon aroma by purge and trap extraction followed by gas chromatography-mass spectrometry determination.
abstract: A dynamic headspace purge-and-trap (DHS-P&T) methodology for the determination and quantification of 61 volatile compounds responsible for muskmelon and watermelon aroma has been developed and validated. The methodology is based on the application of purge-and-trap extraction followed by gas chromatography coupled to (ion trap) mass spectrometry detection. For this purpose two different P&T sorbent cartridges have been evaluated. The influence of different extraction factors (sample weight, extraction time, and purge flow) on extraction efficiency has been studied and optimised using response surface methodology. Precision, expressed as repeatability, has been evaluated by analysing six replicates of real samples, showing relative standard deviations between 3% and 27%. Linearity has been studied in the range of 10-6130 ng mL(-1) depending on the compound response, showing coefficients of correlation between 0.995 and 0.999. Detection limits ranged between 0.1 and 274 ng g(-1). The methodology developed is well suited for analysis of large numbers of muskmelon and watermelon samples in plant breeding programs.
authors.affiliation: Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, E-12071 Castellón, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Fredes
authors.affiliation: Institut Universitari de Plaguicides i Aigües, Universitat Jaume I, Avda. Sos Baynat s/n, E-12071 Castellón, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sales
authors.affiliation: Institut Universitari de Plaguicides i Aigües, Universitat Jaume I, Avda. Sos Baynat s/n, E-12071 Castellón, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Barreda
authors.affiliation: Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, E-12071 Castellón, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Valcárcel
authors.affiliation: Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, E-12071 Castellón, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Roselló
authors.affiliation: Institut Universitari de Plaguicides i Aigües, Universitat Jaume I, Avda. Sos Baynat s/n, E-12071 Castellón, Spain. Electronic address: beltranj@uji.es.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Beltrán
mesh_terms: D029773:Citrullus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D055549:Volatile Organic Compounds / Q000737:chemistry*
chemical_list: D055549:Volatile Organic Compounds
keywords: Cucurbitaceae; GC–MS; Organoleptic quality; P&T; Response surface methodology