title: Induction of ovine trophoblast cell fusion by fematrin-1 in vitro.
abstract: Endogenous retroviruses present in the genomes take a specific role in placental formation in various vertebrates, including bovine and sheep. Fematrin-1, which is the envelope (Env) protein of bovine endogenous retrovirus found in bovine placenta, is involved in the formation of fetomaternal hybrid cells in cattle placenta. This study was conducted to clarify whether fematrin-1 possesses fusogenic activity in trophoblast cells. Another question is whether Env proteins only have species-specific activity or not. For this, fematrin-1 gene was transfected in ovine trophoblast cells, and we examined fusogenic activity with Cos-7 cells. Although fematrin-1 fusogenic activity was detected in both neutral and acidic pH conditions, acidic condition significantly enhanced it. These activities were rather weaker than those of vesicular stomatitis virus G protein as a positive control. However, the ratio of fematrin-1 and vesicular stomatitis virus G protein fusion index was confirmed similar to those in the previous reports. Some fusion cells showed multinucleate cells. These results imply that fematrin-1 is involved in the formation of trophoblast hybrid cells even in different species trophoblastic cells.
authors.affiliation: Laboratory of Veterinary Physiology, Co-Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.|The Niikawa Public Health Center, Kurobe, Japan.
authors.forename: Katsuo
authors.initials: K
authors.lastname: Koshi
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakaya
authors.affiliation: Laboratory of Veterinary Physiology, Co-Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kizaki
authors.affiliation: Integrated Center for Science, Ehime University, Toon, Japan.
authors.forename: Toshina
authors.initials: T
authors.lastname: Ishiguro-Oonuma
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
authors.affiliation: Department of Animal Sciences, Center for Reproductive Biology, Washington State University, Pullman, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Spencer
authors.affiliation: Laboratory of Veterinary Physiology, Co-Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hashizume
mesh_terms: D000818:Animals; D019556:COS Cells; D002417:Cattle; D002459:Cell Fusion; D002522:Chlorocebus aethiops; D020077:Endogenous Retroviruses / Q000235:genetics*; D005260:Female; D015686:Gene Products, env / Q000502:physiology*; D006822:Hybrid Cells; D006863:Hydrogen-Ion Concentration; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D015686:Gene Products, env
keywords: cell fusion; fematrin-1; trophoblast