title: SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.
abstract: Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small-molecule recognition unit (Ni-trisNTA) and the genetically encoded minimal protein His6-10 -tag. High-density protein tracing by SLAP was demonstrated. This technique allows super-resolution fluorescence imaging and fulfills the necessary sampling criteria for single-molecule localization-based imaging techniques. It avoids masking by large probes, for example, antibodies, and supplies sensitive, precise, and robust size analysis of protein clusters (nanodomains).
authors.affiliation: Institute of Biochemistry, Biocenter; Cluster of Excellence Frankfurt, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt/M. (Germany) http://www.biochem.uni-frankfurt.de http://www.smb.uni-frankfurt.de.
authors.forename: Ralph
authors.initials: R
authors.lastname: Wieneke
authors.affiliation: Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt/M. (Germany).
authors.forename: Anika
authors.initials: A
authors.lastname: Raulf
authors.affiliation: Institute of Biochemistry, Biocenter; Cluster of Excellence Frankfurt, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt/M. (Germany) http://www.biochem.uni-frankfurt.de http://www.smb.uni-frankfurt.de.
authors.forename: Alina
authors.initials: A
authors.lastname: Kollmannsperger
authors.affiliation: Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt/M. (Germany). heilemann@chemie.uni-frankfurt.de.
authors.forename: Mike
authors.initials: M
authors.lastname: Heilemann
authors.affiliation: Institute of Biochemistry, Biocenter; Cluster of Excellence Frankfurt, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt/M. (Germany) http://www.biochem.uni-frankfurt.de http://www.smb.uni-frankfurt.de. tampe@em.uni-frankfurt.de.
authors.forename: Robert
authors.initials: R
authors.lastname: Tampé
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D034904:Lamin Type A / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D036103:Nanotechnology
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D034904:Lamin Type A
keywords: bioorthogonal chemistry; chemical biology; proteins; single-molecule imaging; super-resolution microscopy