title: System for evaluation of oxidative stress on in-vitro-produced bovine embryos.
abstract: This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O2, were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5 µmol/l) for 24 h. Blastocyst rate (BLR) was recorded and expanded blastocysts were stained with CellROX®Green (CRG; OS evaluation) and evaluated under epifluorescence microscopy (ratio of pixel/blastomere). A significant effect of menadione was observed for BLR (P = 0.0039), number of blastomeres/embryo (P < 0.0001) and OS (P < 0.001). Strong negative correlations were found between BLR and the number of blastomeres with OS evaluation, demonstrating the efficacy of this analysis to evaluate OS levels of IVF bovine embryos.
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
authors.forename: Patricia Monken
authors.initials: PM
authors.lastname: de Assis
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
authors.forename: Leticia Signori
authors.initials: LS
authors.lastname: Castro
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
authors.forename: Adriano Felipe Perez
authors.initials: AF
authors.lastname: Siqueira
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
authors.forename: Juliana de Carvalho
authors.initials: Jde C
authors.lastname: Delgado
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
authors.forename: Thais Rose Dos Santos
authors.initials: TR
authors.lastname: Hamilton
authors.affiliation: Laboratory of in vitro Fertilization, Cloning and Animal Transgenesis, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction, University of São Paulo, Brazil.
authors.forename: Marcelo Demarchi
authors.initials: MD
authors.lastname: Goissis
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil; Laboratory of in vitro Fertilization, Cloning and Animal Transgenesis, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction, University of São Paulo, Brazil.
authors.forename: Camilla Mota
authors.initials: CM
authors.lastname: Mendes
authors.affiliation: Laboratory of Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
authors.forename: Marcilio
authors.initials: M
authors.lastname: Nichi
authors.affiliation: Laboratory of in vitro Fertilization, Cloning and Animal Transgenesis, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction, University of São Paulo, Brazil.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Visintin
authors.affiliation: Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil. Electronic address: meoaa@usp.br.
authors.forename: Mayra Elena Ortiz D'Ávila
authors.initials: ME
authors.lastname: Assumpção
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001757:Blastomeres / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000187:drug effects; D024483:Vitamin K 3 / Q000633:toxicity
chemical_list: D024483:Vitamin K 3
keywords: CellROX®Green; development; embryo oxidative stress; microscopy of epifluorescence