title: Preinduction of heat shock protein 70 protects mice against post-infection irritable bowel syndrome via NF-κB and NOS/NO signaling pathways.
abstract: This study aimed to investigate the protective effects of preinduction of heat shock protein 70 (HSP70) on Trichinella spiralis infection-induced post-infectious irritable bowel syndrome (PI-IBS) in mice. Trichinella spiralis infection significantly reduced HSP70 abundance, ileal villus height and crypt depth, expression of tight junctions, serum lysine and arginine concentrations, and ileal SCL7A6 and SCL7A7 mRNA levels, induced inflammatory response, and activated NF-κB signaling pathway. Meanwhile, the heat treatment upregulated HSP70 expression, and then reversed intestinal dysfunction and inflammatory response. Preinduction of HSP70 enhanced serum arginine and intestinal SCL7A7 expression and inhibited NF-κB activation compared with PI-IBS model. Treatment with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) and N-nitro-L-arginine methyl ester hydrochloride (L-NAME, a nitric oxide synthase inhibitor, NOS) further demonstrated that preinduction of HSP70 might inhibit NF-κB and activated NOS/nitric oxide (NO) signaling pathways. In conclusion, preinduction of HSP70 by heat treatment may confer beneficial effects on Trichinella spiralis infection-induced PI-IBS in mice, and the protective effect of HSP70 may be associated with inhibition of NF-κB and stimulation of NOS/NO signaling pathways.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Xuchun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China.
authors.forename: Zhoutao
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China.
authors.forename: Yiyao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China.
authors.forename: Taozhi
authors.initials: T
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China.
authors.forename: Baili
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China. forweier0731@126.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lan
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood; D027182:Amino Acid Transport System y+ / Q000235:genetics; D027241:Amino Acid Transport System y+L; D026923:Amino Acid Transport Systems, Basic / Q000235:genetics; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001120:Arginine / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007249:Inflammation; D007413:Intestinal Mucosa / Q000378:metabolism; D043183:Irritable Bowel Syndrome / Q000378:metabolism* / Q000469:parasitology; D008070:Lipopolysaccharides / Q000097:blood; D008239:Lysine / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000737:chemistry; D009569:Nitric Oxide / Q000097:blood*; D010539:Permeability; D011392:Proline / Q000031:analogs & derivatives / Q000737:chemistry; D011897:Random Allocation; D015398:Signal Transduction; D013859:Thiocarbamates / Q000737:chemistry; D019108:Tight Junctions / Q000378:metabolism; D017160:Trichinella spiralis; D014235:Trichinellosis / Q000378:metabolism
chemical_list: D027182:Amino Acid Transport System y+; D027241:Amino Acid Transport System y+L; D026923:Amino Acid Transport Systems, Basic; D000596:Amino Acids; D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; C542311:LAT3 protein, mouse; D008070:Lipopolysaccharides; D016328:NF-kappa B; C518287:Slc7a7 protein, mouse; D013859:Thiocarbamates; C066229:prolinedithiocarbamate; D009569:Nitric Oxide; D001120:Arginine; D011392:Proline; D006631:Amine Oxidase (Copper-Containing); D008239:Lysine; D019331:NG-Nitroarginine Methyl Ester
keywords: HSP70; Inflammation; Mice; NF-κB; NOS/NO; PI-IBS