title: Reducible chimeric polypeptide consisting of octa-D-arginine and tetra-L-histidine peptides as an efficient gene delivery vector.
abstract: Cationic oligopeptide as a nonviral gene delivery vector has aroused much research interest recently, but its further application is limited by its low transfection efficiency. In the present study, we have created a high-efficiency gene vector by using octa-D-arginine and tetra-L-histidine to form a disulfide cross-linked chimeric polypeptide and used this vector to deliver the therapeutic gene tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) to see whether the gene could be transferred and could exert antitumor effects in vitro and in vivo. The result showed that the newly designed vector was able to condense DNA into nanosized polyplexes effectively, thus facilitating its transmembrane transport, promoting its endosomal escape, and finally enabling degradation within the cell. Our study has demonstrated that this chimeric polypeptide is an effective gene carrier in cancer therapy.
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China ; Department of Pharmaceutics, ChengDu Military General Hospital, ChengDu, People's Republic of China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Zongguang
authors.initials: Z
authors.lastname: Tai
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Pharmaceutics, Shanghai Pulmonary Hospital, Tongji University, Shanghai, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Chong
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Quangang
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Shen
authors.initials: S
authors.lastname: Gao
mesh_terms: D000970:Antineoplastic Agents; D018014:Gene Transfer Techniques; D006639:Histidine / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D009842:Oligopeptides; D053221:TNF-Related Apoptosis-Inducing Ligand; C500307:octa-arginine peptide; C033223:polyhistidine; D006639:Histidine
keywords: TRAIL; disulfide bond; octa-d-arginine; reducible polypeptide; tetra-l-histidine