title: Identification and Characterization of CD300H, a New Member of the Human CD300 Immunoreceptor Family.
abstract: Recruitment of circulating monocytes and neutrophils to infection sites is essential for host defense against infections. Here, we identified a previously unannotated gene that encodes an immunoglobulin-like receptor, designated CD300H, which is located in the CD300 gene cluster. CD300H has a short cytoplasmic tail and associates with the signaling adaptor proteins, DAP12 and DAP10. CD300H is expressed on CD16(+) monocytes and myeloid dendritic cells. Ligation of CD300H on CD16(+) monocytes and myeloid dendritic cells with anti-CD300H monoclonal antibody induced the production of neutrophil chemoattractants. Interestingly, CD300H expression varied among healthy subjects, who could be classified into two groups according to "positive" and "negative" expression. Genomic sequence analysis revealed a single-nucleotide substitution (rs905709 (G → A)) at a splice donor site on intron 1 on either one or both alleles. The International HapMap Project database has demonstrated that homozygosity for the A allele of single nucleotide polymorphism (SNP) rs905709 ("negative" expression) is highly frequent in Han Chinese in Beijing, Japanese in Tokyo, and Europeans (A/A genotype frequencies 0.349, 0.167, and 0.138, respectively) but extremely rare in Sub-Saharan African populations. Together, these results suggest that CD300H may play an important role in innate immunity, at least in populations that carry the G/G or G/A genotype of CD300H.
authors.affiliation: From the Departments of Immunology and the Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Kouta
authors.initials: K
authors.lastname: Niizuma
authors.affiliation: From the Departments of Immunology and the Center for Tsukuba Advanced Research Alliance (TARA), the Japan Science and Technology Agency, CREST, and tokothr@md.tsukuba.ac.jp.
authors.forename: Satoko
authors.initials: S
authors.lastname: Tahara-Hanaoka
authors.affiliation: Medical Genetics, Faculty of Medicine, the Japan Science and Technology Agency, CREST, and.
authors.forename: Emiko
authors.initials: E
authors.lastname: Noguchi
authors.affiliation: From the Departments of Immunology and the Center for Tsukuba Advanced Research Alliance (TARA), the Japan Science and Technology Agency, CREST, and.
authors.forename: Akira
authors.initials: A
authors.lastname: Shibuya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D044466:Asian People / Q000235:genetics; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D020298:U937 Cells; D044465:White People / Q000235:genetics
chemical_list: C000603983:CD300H protein, human; D016207:Cytokines; D020033:Protein Isoforms; D011971:Receptors, Immunologic
keywords: chemokine; dendritic cell; monocyte; neutrophil; single-nucleotide polymorphism (SNP)