title: Herbivory-induced glucose transporter gene expression in the brown planthopper, Nilaparvata lugens.
abstract: Nilaparvata lugens, the brown planthopper (BPH) feeds on rice phloem sap, containing high amounts of sucrose as a carbon source. Nutrients such as sugars in the digestive tract are incorporated into the body cavity via transporters with substrate selectivity. Eighteen sugar transporter genes of BPH (Nlst) were reported and three transporters have been functionally characterized. However, individual characteristics of NlST members associated with sugar transport remain poorly understood. Comparative gene expression analyses using oligo-microarray and quantitative RT-PCR revealed that the sugar transporter gene Nlst16 was markedly up-regulated during BPH feeding. Expression of Nlst16 was induced 2 h after BPH feeding on rice plants. Nlst16, mainly expressed in the midgut, appears to be involved in carbohydrate incorporation from the gut cavity into the hemolymph. Nlst1 (NlHT1), the most highly expressed sugar transporter gene in the midgut was not up-regulated during BPH feeding. The biochemical function of NlST16 was shown as facilitative glucose transport along gradients. Glucose uptake activity by NlST16 was higher than that of NlST1 in the Xenopus oocyte expression system. At least two NlST members are responsible for glucose uptake in the BPH midgut, suggesting that the midgut of BPH is equipped with various types of transporters having diversified manner for sugar uptake.
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan; Department of Integrated Biosciences, Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan. Electronic address: singo@cc.tuat.ac.jp.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kikuta
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan; Department of Integrated Biosciences, Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kikawada
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan; Department of Integrated Biosciences, Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. Electronic address: hnada@affrc.go.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Noda
mesh_terms: D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D051246:Glucose Transport Proteins, Facilitative / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006430:Hemiptera / Q000235:genetics / Q000378:metabolism*; D060434:Herbivory; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes; D012275:Oryza; D052585:Phloem; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D013395:Sucrose / Q000378:metabolism; D014981:Xenopus
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D019476:Insect Proteins; D013395:Sucrose
keywords: Brown planthopper; Gene expression; Glucose transporter; Laser stylectomy; Microarray; Midgut; Nilaparvata lugens; Xenopus oocyte