title: Optimization of advanced Wiener estimation methods for Raman reconstruction from narrow-band measurements in the presence of fluorescence background.
abstract: Raman spectroscopy has shown great potential in biomedical applications. However, intrinsically weak Raman signals cause slow data acquisition especially in Raman imaging. This problem can be overcome by narrow-band Raman imaging followed by spectral reconstruction. Our previous study has shown that Raman spectra free of fluorescence background can be reconstructed from narrow-band Raman measurements using traditional Wiener estimation. However, fluorescence-free Raman spectra are only available from those sophisticated Raman setups capable of fluorescence suppression. The reconstruction of Raman spectra with fluorescence background from narrow-band measurements is much more challenging due to the significant variation in fluorescence background. In this study, two advanced Wiener estimation methods, i.e. modified Wiener estimation and sequential weighted Wiener estimation, were optimized to achieve this goal. Both spontaneous Raman spectra and surface enhanced Raman spectra were evaluated. Compared with traditional Wiener estimation, two advanced methods showed significant improvement in the reconstruction of spontaneous Raman spectra. However, traditional Wiener estimation can work as effectively as the advanced methods for SERS spectra but much faster. The wise selection of these methods would enable accurate Raman reconstruction in a simple Raman setup without the function of fluorescence suppression for fast Raman imaging.
authors.affiliation: Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 637457, Singapore.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 637457, Singapore.
authors.forename: Yi Hong
authors.initials: YH
authors.lastname: Ong
authors.affiliation: Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 637457, Singapore ; Fujian Normal Univeristy, Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fuzhou, 350007, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Lin
authors.affiliation: Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 637457, Singapore.
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
keywords: (110.4234) Multispectral and hyperspectral imaging; (170.3880) Medical and biological imaging; (170.5660) Raman spectroscopy; (240.6695) Surface-enhanced Raman scattering