title: Methods to Study the Role of the Glycocalyx in the Uptake of Cell-Penetrating Peptides.
abstract: Cells are covered by a layer of negatively charged oligo- and polysaccharides, the glycocalyx. Cell-penetrating peptides and other drug delivery vehicles first encounter these polyanions before contacting the lipid bilayer of the plasma membrane. While a large body of data supports the notion that interactions with the glycocalyx promote or even trigger uptake, in some cases, the glycocalyx compromises delivery. As a consequence there is a need to address the role of the glycocalyx in delivery for each specific delivery vehicle and for each particular type of cell. Here, we describe protocols to obtain information on the composition and dynamics of the glycocalyx, and the role of individual glycocalyx components in the uptake of drug delivery vehicles.
authors.affiliation: Department of Biochemistry, Radboud University Medical Center, Geert Grooteplein 28, 6525, GA, Nijmegen, The Netherlands.
authors.forename: Samuel
authors.initials: S
authors.lastname: Schmidt
authors.forename: Rike
authors.initials: R
authors.lastname: Wallbrecher
authors.forename: Toin H
authors.initials: TH
authors.lastname: van Kuppevelt
authors.forename: Roland
authors.initials: R
authors.lastname: Brock
mesh_terms: D000818:Animals; D057846:Cell-Penetrating Peptides / Q000032:analysis / Q000378:metabolism*; D057930:Click Chemistry; D004337:Drug Carriers / Q000032:analysis / Q000378:metabolism*; D016503:Drug Delivery Systems; D005455:Fluorescent Antibody Technique / Q000379:methods; D019276:Glycocalyx / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D057127:Single-Chain Antibodies / Q000032:analysis
chemical_list: D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; D057127:Single-Chain Antibodies; D019158:N-Acetylneuraminic Acid