title: Identification of Conserved and Species-Specific Functions of the Listeria monocytogenes PrsA2 Secretion Chaperone.
abstract: The Gram-positive bacterium Listeria monocytogenes is a facultative intracellular pathogen that relies on the regulated secretion and activity of a variety of proteins that sustain life within diverse environments. PrsA2 has recently been identified as a secreted peptidyl-prolyl cis/trans isomerase and chaperone that is dispensable for bacterial growth in broth culture but essential for L. monocytogenes virulence. Following host infection, PrsA2 contributes to the proper folding and activity of secreted proteins that are required for bacterial replication within the host cytosol and for bacterial spread to adjacent cells. PrsA2 is one member of a family of Gram-positive secretion chaperones that appear to play important roles in bacterial physiology; however, it is not known how these proteins recognize their substrate proteins or the degree to which their function is conserved across diverse Gram-positive species. We therefore examined PrsA proteins encoded by a variety of Gram-positive bacteria for functional complementation of L. monocytogenes mutants lacking prsA2. PrsA homologues encoded by Bacillus subtilis, Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcus mutans, Staphylococcus aureus, and Lactococcus lactis were examined for functional complementation of a variety of L. monocytogenes PrsA2-associated phenotypes central to L. monocytogenes pathogenesis and bacterial cell physiology. Our results indicate that while selected aspects of PrsA2 function are broadly conserved among diverse Gram-positive bacteria, PrsA2 exhibits unique specificity for L. monocytogenes target proteins required for pathogenesis. The L. monocytogenes PrsA2 chaperone thus appears evolutionarily optimized for virulence factor secretion within the host cell cytosol while still maintaining aspects of activity relevant to more general features of Gram-positive protein translocation.
authors.affiliation: Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Laty A
authors.initials: LA
authors.lastname: Cahoon
authors.affiliation: Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA nfreitag@uic.edu.
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Freitag
mesh_terms: D000595:Amino Acid Sequence; D058947:Bacterial Secretion Systems; D017124:Conserved Sequence; D019143:Evolution, Molecular; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000145:classification / Q000201:enzymology / Q000235:genetics; D008089:Listeria monocytogenes / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D008088:Listeriosis; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D013045:Species Specificity; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D058947:Bacterial Secretion Systems; D018832:Molecular Chaperones; D037521:Virulence Factors; D019696:Peptidylprolyl Isomerase; C541924:PrsA2 protein, Listeria monocytogenes