title: Blood Vessel Maturation by Disintegrin in Oxygen-Induced Retinopathy.
abstract: Although Arg-Gly-Asp (RGD) motif-containing disintegrins are associated with integrin inhibition and the activation of various biological processes, little is known about the role of RGD motif-containing disintegrin in vascular development and remodeling. We therefore investigated the role of RGD-containing disintegrin in vascular remodeling in oxygen-induced retinopathy (OIR) mouse model.
  
  
  
  EGT022, an RGD-containing disintegrin originated from human a disintegrin and metalloproteinase 15 (ADAM15), was used to investigate the role of the disintegrin in vascular development in OIR mouse model. To analyze the functional effects of EGT022 on retinal vascular development, the immunohistochemistry on mouse retinas after fluorescein isothiocyanate (FITC) perfusion was conducted and the vessel integrity was examined using modified Mile's permeability assay.
  
  
  
  EGT022 was able to reduce overall retinopathy scores by 75%, indicating its efficacy in retinal microvessel maturation stimulation. Pericyte coverage was greatly stimulated by EGT022 treatment in OIR mouse model. EGT022 was also effective to significantly improve blood vessel integrity.
  
  
  
  RGD-containing disintegrin EGT022 stimulated vascular maturation in OIR mouse model. Experimental results suggest that EGT022 is useful for treatments to improve ischemia in nonproliferative diabetic retinopathy (NPDR), the early stage of diabetic retinopathy.
authors.affiliation: a R&D Center, EyeGene Inc. , Seoul , Republic of Korea .|b Department of Biochemistry , College of Life Science and Biotechnology, Yonsei University , Seoul , Korea .
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Jang
authors.affiliation: a R&D Center, EyeGene Inc. , Seoul , Republic of Korea .
authors.forename: Yang Je
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: a R&D Center, EyeGene Inc. , Seoul , Republic of Korea .
authors.forename: Hyun Jong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: a R&D Center, EyeGene Inc. , Seoul , Republic of Korea .
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: c Department of Ophthalmology , Soonchunhyang University College of Medicine , Seoul , Korea and.
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: d Nune Eye Hospital , Seoul , Korea.
authors.forename: Oh Woong
authors.initials: OW
authors.lastname: Kwon
authors.affiliation: b Department of Biochemistry , College of Life Science and Biotechnology, Yonsei University , Seoul , Korea .
authors.forename: Doo-Sik
authors.initials: DS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D019483:Disintegrins / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D015861:Retinal Neovascularization / Q000473:pathology*; D012171:Retinal Vessels / Q000187:drug effects / Q000473:pathology*; D066253:Vascular Remodeling / Q000187:drug effects*
chemical_list: D019483:Disintegrins; D010975:Platelet Aggregation Inhibitors; D010100:Oxygen
keywords: Diabetic retinopathy; disintegrin; integrin; oxygen-induced retinopathy; retinal vessel