title: Pharmacokinetics studies of enrofloxacin injectable in situ forming gel in dogs.
abstract: The objective of this study was to evaluate the pharmacokinetic characteristics of enrofloxacin (ENR) injectable in situ gel we developed in dogs following a single intramuscular (i.m.) administration. Twelve healthy dogs were randomly divided into two groups (six dogs per group), then administrated a single 20 mg/kg body weight (b.w.) ENR injectable in situ gel and a single 5 mg/kg b.w. ENR conventional injection, respectively. High-performance liquid chromatography (HPLC) was used to determine ENR plasma concentrations. The pharmacokinetic parameters of ENR injectable in situ gel and conventional injection in dogs are as follows: MRT (mean residence time) (45.59 ± 14.05) h verse (11.40 ± 1.64) h, AUC (area under the blood concentration vs. time curve) (28.66 ± 15.41) μg·h/mL verse (11.06 ± 3.90) μg·h/mL, cmax (maximal concentration) (1.59 ± 0.35) μg/mL verse (1.46 ± 0.07) μg/mL, tmax (time needed to reach cmax ) (1.25 ± 1.37) h verse (1.40 ± 0.55) h, t1/2λz (terminal elimination half-life) (40.27 ± 17.79) h verse (10.32 ± 0.97) h. The results demonstrated that the in situ forming gel system could increase dosing interval of ENR and thus reduced dosing frequency during long-term treatment. Therefore, the ENR injectable in situ gel seems to be worth popularizing in veterinary clinical application.
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: H-M
authors.initials: HM
authors.lastname: Li
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Tian
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Z-R
authors.initials: ZR
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Luo
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Z-G
authors.initials: ZG
authors.lastname: Yu
mesh_terms: D000818:Animals; D019540:Area Under Curve; D003692:Delayed-Action Preparations; D004285:Dogs / Q000097:blood*; D010450:Endopeptidases; D056827:Endosomal Sorting Complexes Required for Transport; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D005782:Gels; D006207:Half-Life; D007273:Injections, Intramuscular / Q000662:veterinary; D015394:Molecular Structure; D029701:Saccharomyces cerevisiae Proteins; D043222:Ubiquitin Thiolesterase
chemical_list: C084434:DOA4 protein, S cerevisiae; D003692:Delayed-Action Preparations; D056827:Endosomal Sorting Complexes Required for Transport; D024841:Fluoroquinolones; D005782:Gels; D029701:Saccharomyces cerevisiae Proteins; D000077422:Enrofloxacin; D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase