title: Clinical potential of miR-3651 as a novel prognostic biomarker for esophageal squamous cell cancer.
abstract: Accumulating evidence indicates that dysregulated microRNA-3651(miR-3651) is involved in tumorigenesis and cancer progression. In this study, we investigated the expression of miR-3651 in esophageal squamous cell cancer(ESCC) and its relationship with tumor progression and clinical prognosis. The expression level of miR-3651 was examined by quantitative Real-time PCR (qRT-PCR) in fresh ESCC tissues and FFPE tissues. The correlation between miR-3651 expression and clinical features and prognosis were statistically analyzed. The results showed that the miR-3651 expression was significantly down-regulated in tumor tissues compared with the paracancerous tissues. Moreover, miR-3651 expression was negatively correlated with T stage of ESCC (P = 0.022) and tumor length (P = 0.015). Kaplan-Meier analysis demonstrated that low miR-3651 expression level was associated with poorer overall survival (OS) (P = 0.004) and disease-free survival (DFS) (P = 0.001). Multivariate analysis identified miR-3651 expression as independent prognostic factor for OS and DFS (P = 0.001 and P = 0.001, resp.). Further stratified analysis revealed the significant association between low miR-3651 expression and worse survival in early patients, but not in the advanced patients. Taken together, miR-3651 was down-regulated in cancerous tissues of ESCC. It may play an important role in cancer progression and could be used as an independent prognostic biomarker for ESCC patients.
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Shanghui
authors.initials: S
authors.lastname: Guan
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Image, Shandong Medical College, Jinan, Shandong, 250002, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Effat
authors.initials: E
authors.lastname: Un Nesa
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Qingxu
authors.initials: Q
authors.lastname: Song
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Cihang
authors.initials: C
authors.lastname: Bao
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China.
authors.forename: Xintong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, PR China. Electronic address: qlchengyufeng@163.com.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000503:physiopathology; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008840:Microfilament Proteins; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011379:Prognosis; D016601:RNA-Binding Proteins; D012307:Risk Factors; D012907:Smoking / Q000503:physiopathology; D016019:Survival Analysis; D047368:Tumor Burden; D059016:Tumor Microenvironment / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C484440:Frg1 protein, mouse; D035683:MicroRNAs; D008840:Microfilament Proteins; D016601:RNA-Binding Proteins
keywords: ESCC; Prognosis; RT-PCR; miR-3651