title: Probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China.
abstract: The purpose of this study was to evaluate the probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China. Four Enterococcus isolates showed high cholesterol removal ability in media were identified as Enterococcus durans (KLDS 6.0930 and 6.0933) and Enterococcus faecalis (KLDS 6.0934 and 6.0935) by 16S rRNA and pheS gene sequences, respectively, and selected for further evaluation. In order to assess the probiotic potential and safety of these strains, the property of four Enterococcus strains were examined, including acid and bile tolerance, adherence to Caco-2 cells and antibiotics susceptibility. All four strains showed potential cholesterol assimilation, de-conjugation of bile salts and/or cholesterol degradation to remove cholesterol in vitro. In addition, the potential effect of E. durans KLDS 6.0930 on serum cholesterol levels was evaluated in Sprague-Dawley rats. After 4 weeks administration, compared with rats fed a high-cholesterol diet without lactic acid bacteria supplementation, there was a significant (P < 0.05) decrease in the total cholesterol and low-density lipoprotein cholesterol levels in the serum of rats treated with KLDS 6.0930. Furthermore, total bile acid level in the feces was significantly (P < 0.05) increased after KLDS 6.0930 administration. These observations suggested that the strain E. durans KLDS 6.0930 may be used in the future as a good candidate for lowering human serum cholesterol levels.
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China.|College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China.
authors.forename: Yaru
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, China. gchuo1958@yahoo.com.
authors.forename: Guicheng
authors.initials: G
authors.lastname: Huo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001647:Bile Acids and Salts / Q000032:analysis; D001711:Biotransformation; D018938:Caco-2 Cells; D002448:Cell Adhesion; D002681:China; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016983:Enterococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology; D005243:Feces / Q000737:chemistry; D005516:Food Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000008:administration & dosage*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D044967:Serum / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002784:Cholesterol