title: Circulating miR-200-family micro-RNAs have altered plasma levels in patients with endometriosis and vary with blood collection time.
abstract: OBJECTIVE
  To determine whether circulating micro-RNA (miR) 200a, miR-200b, and miR-141 have altered levels in patients with endometriosis compared with control individuals.
  
  
  METHODS
  Experimental laboratory study.
  
  
  METHODS
  University.
  
  
  METHODS
  Patients with endometriosis (n = 61), laparoscopically confirmed endometriosis-free women (n = 35), and self-reported healthy women (n = 30) were included in the study.
  
  
  METHODS
  None.
  
  
  METHODS
  Plasma miRNA levels in endometriosis patients and control subjects.
  
  
  RESULTS
  We found that the levels of studied miRNAs varied with blood collection time, being lower in the morning than in the evening. When blood collection time was taken into account, the results revealed significantly lower levels of miR-200a and miR-141 in the evening plasma samples of women with endometriosis compared with surgically confirmed disease-free patients. However, the evening-sample levels of all three miRNAs were significantly lower in patients with stage I-II endometriosis than in endometriosis-free control subjects. In cases of stage III-IV endometriosis, only miR-200a levels were significantly lower compared with patients without endometriosis. Circulating miR-200a showed the best discriminative power to differentiate women with endometriosis from patients with similar complaints but without the disease.
  
  
  CONCLUSIONS
  Our findings suggest that miR-200a and miR-141 have a potential as novel noninvasive biomarkers for endometriosis. In addition, we found that the plasma miR-200a, miR-200b and miR-141 levels vary with blood sampling time, so it is important to take the sample collection time into account when studying miRNAs as biomarkers.
authors.affiliation: Department of Obstetrics and Gynecology, University of Tartu, Tartu, Estonia; Competence Center on Health Technologies, Tartu, Estonia.
authors.forename: Kadri
authors.initials: K
authors.lastname: Rekker
authors.affiliation: Department of Obstetrics and Gynecology, University of Tartu, Tartu, Estonia; Competence Center on Health Technologies, Tartu, Estonia; Institute of Bio- and Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Merli
authors.initials: M
authors.lastname: Saare
authors.affiliation: Competence Center on Health Technologies, Tartu, Estonia.
authors.forename: Anne Mari
authors.initials: AM
authors.lastname: Roost
authors.affiliation: Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Tartu, Estonia.
authors.forename: Tanel
authors.initials: T
authors.lastname: Kaart
authors.affiliation: Competence Center on Health Technologies, Tartu, Estonia; Tartu University Hospital's Women's Clinic, Tartu, Estonia; Elite Clinic, Tartu, Estonia.
authors.forename: Deniss
authors.initials: D
authors.lastname: Sõritsa
authors.affiliation: Department of Obstetrics and Gynecology, University of Tartu, Tartu, Estonia; Tartu University Hospital's Women's Clinic, Tartu, Estonia.
authors.forename: Helle
authors.initials: H
authors.lastname: Karro
authors.affiliation: Elite Clinic, Tartu, Estonia.
authors.forename: Andrei
authors.initials: A
authors.lastname: Sõritsa
authors.affiliation: Fundación Instituto Valenciano de Infertilidad, Instituto Universitario IVI, University of Valencia, Valencia, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.affiliation: Department of Obstetrics and Gynecology, University of Tartu, Tartu, Estonia; Competence Center on Health Technologies, Tartu, Estonia; Institute of Bio- and Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Andres
authors.initials: A
authors.lastname: Salumets
authors.affiliation: Department of Obstetrics and Gynecology, University of Tartu, Tartu, Estonia; Competence Center on Health Technologies, Tartu, Estonia. Electronic address: maire.peters@ut.ee.
authors.forename: Maire
authors.initials: M
authors.lastname: Peters
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D005810:Multigene Family; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; C533895:MIRN141 microRNA, human; C533896:MIRN200 microRNA, human; D035683:MicroRNAs
keywords: biomarker; circadian rhythm; endometriosis; miR-200 family; miRNA