title: Effects of hydrostatic pressure on yeasts isolated from deep-sea hydrothermal vents.
abstract: Hydrostatic pressure plays a significant role in the distribution of life in the biosphere. Knowledge of deep-sea piezotolerant and (hyper)piezophilic bacteria and archaea diversity has been well documented, along with their specific adaptations to cope with high hydrostatic pressure (HHP). Recent investigations of deep-sea microbial community compositions have shown unexpected micro-eukaryotic communities, mainly dominated by fungi. Molecular methods such as next-generation sequencing have been used for SSU rRNA gene sequencing to reveal fungal taxa. Currently, a difficult but fascinating challenge for marine mycologists is to create deep-sea marine fungus culture collections and assess their ability to cope with pressure. Indeed, although there is no universal genetic marker for piezoresistance, physiological analyses provide concrete relevant data for estimating their adaptations and understanding the role of fungal communities in the abyss. The present study investigated morphological and physiological responses of fungi to HHP using a collection of deep-sea yeasts as a model. The aim was to determine whether deep-sea yeasts were able to tolerate different HHP and if they were metabolically active. Here we report an unexpected taxonomic-based dichotomic response to pressure with piezosensitve ascomycetes and piezotolerant basidiomycetes, and distinct morphological switches triggered by pressure for certain strains.
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané, France. Electronic address: gaetan.burgaud@univ-brest.fr.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Burgaud
authors.affiliation: Université de Bourgogne, AgroSup Dijon, Dimacell Imaging Ressource Center, UMR A 02.102 PAM, F-21000 Dijon, France.
authors.forename: Nguyen Thi Minh
authors.initials: NT
authors.lastname: Hué
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Danielle
authors.initials: D
authors.lastname: Arzur
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Monika
authors.initials: M
authors.lastname: Coton
authors.affiliation: Université de Bourgogne, AgroSup Dijon, Dimacell Imaging Ressource Center, UMR A 02.102 PAM, F-21000 Dijon, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Perrier-Cornet
authors.affiliation: Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; CNRS, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; Ifremer, Centre de Brest, REM/EEP/LM2E, Technopôle Brest-Iroise, BP70, 29 280 Plouzané, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jebbar
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Barbier
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D060086:Hydrothermal Vents / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*; D013312:Stress, Physiological / Q000235:genetics
keywords: Dimorphism; Filamentation; Hydrostatic pressure; Marine; Piezotolerance; Yeasts