title: Design of Decorin-Based Peptides That Bind to Collagen I and their Potential as Adhesion Moieties in Biomaterials.
abstract: Mimicking the binding epitopes of protein-protein interactions by using small peptides is important for generating modular biomimetic systems. A strategy is described for the design of such bioactive peptides without accessible structural data for the targeted interaction, and the effect of incorporating such adhesion peptides in complex biomaterial systems is demonstrated. The highly repetitive structure of decorin was analyzed to identify peptides that are representative of the inner and outer surface, and it was shown that only peptides based on the inner surface of decorin bind to collagen. The peptide with the highest binding affinity for collagen I, LHERHLNNN, served to slow down the diffusion of a conjugated dye in a collagen gel, while its dimer could physically crosslink collagen, thereby enhancing the elastic modulus of the gel by one order of magnitude. These results show the potential of the identified peptides for the design of biomaterials for applications in regenerative medicine.
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstrasse 55, 14513 Teltow (Germany).|Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm (Germany).
authors.forename: Stefania
authors.initials: S
authors.lastname: Federico
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstrasse 55, 14513 Teltow (Germany).
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Pierce
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstrasse 55, 14513 Teltow (Germany).|Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm (Germany).
authors.forename: Susanna
authors.initials: S
authors.lastname: Piluso
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstrasse 55, 14513 Teltow (Germany).
authors.forename: Christian
authors.initials: C
authors.lastname: Wischke
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstrasse 55, 14513 Teltow (Germany).|Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm (Germany).
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstrasse 55, 14513 Teltow (Germany). axel.neffe@hzg.de.|Institute of Chemistry, University of Potsdam, 14476 Potsdam-Golm (Germany). axel.neffe@hzg.de.
authors.forename: Axel T
authors.initials: AT
authors.lastname: Neffe
mesh_terms: D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry*; D058575:Decorin / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D058575:Decorin; D010455:Peptides
keywords: biomaterials; collagen; gels; peptides; protein-protein interactions