title: [Analysis of proteomic profile changes of zebrafish embryos during exposure to doxorubicin, built-in the phospholipid transport nanosystem].
abstract: The proteome profile of Danio rerio embryos grown in the medium containing doxorubicin, included in the phospholipid transport nanosystem (doxolip) has been investigated using combination of 1D-electrophoresis with subsequent MALDI-TOF-PMF mass spectrometry. Cultivation of growing of D. rerio embryos in the medium with doxolip caused a substantial increase in expression of the cytoskeletal proteins, a decrease in the number of nuclear proteins involved in DNA and RNA synthesis and disappearance of vitellogenin 2 in comparison with control (the cultivation medium containing the phospholipid transport nanosystem). Analysis of the proteomic profiles of doxolip-treated embryos suggests lower toxicity of doxorubicin incorporated in the phospholipid nanosystem.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Samenkova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Y S
authors.initials: YS
authors.lastname: Kisrieva
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Petushkova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: G P
authors.initials: GP
authors.lastname: Kuznetsova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Larina
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: O P
authors.initials: OP
authors.lastname: Trifonova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Karuzina
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: O M
authors.initials: OM
authors.lastname: Ipatova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Lisitsa
mesh_terms: D000818:Animals; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage; D010743:Phospholipids / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014819:Vitellogenins / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D010743:Phospholipids; D014819:Vitellogenins; D029961:Zebrafish Proteins; C486450:vtg1 protein, zebrafish; D004317:Doxorubicin
keywords: Danio rerio; doxolip; doxorubicin; mass spectrometry; one-dimensional gel electrophoresis; phospholipid nanoparticles