title: Effect of quercetin against mixture of four organophosphate pesticides induced nephrotoxicity in rats.
abstract: 1. It has been demonstrated that the ingestion of foods containing quercetin protects against the toxicity of single pesticides. The aim of this study is to make a comprehensive elaboration about the protective effect of quercetin against multi-organophosphorous pesticides induced nephrotoxicity by measuring indices in rat kidney, urine and serum. Rats were divided into six groups (n = 10/group): control, two different doses of quercetin, pesticide mixture (PM), and different doses of quercetin plus PM-treated groups. 2. The following parameters were significantly changed in PM-treated groups compared with the control (p < 0.01). In kidney, malondialdehyde level raised; catalase, superoxide dismutase activities and glutathione levels were decreased. Comet assay of nephrocytes showed that the proportion of DNA in the tail and tail length increased. In urine, β2-microglobulin, retinol-conjugated protein levels and N-acetyl-β-D-glucosaminidase activity showed increasing response; meanwhile uric acid level was decreased. In serum, creatinine and urea nitrogen levels were increased. However, the anomaly changes of indexes mentioned above in PM-treated group were alleviated when simultaneously administrated with 50 mg/kg body weight/day quercetin (p < 0.05). 3. From the present findings, it can be evaluated that quercetin may protect against adverse effects resulted from multi-organophosphorous pesticides with significant high levels of uptake in man provided.
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Sifan
authors.initials: S
authors.lastname: Li
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Can
authors.initials: C
authors.lastname: Cao
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Haidan
authors.initials: H
authors.lastname: Shi
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qi
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Changhao
authors.initials: C
authors.lastname: Sun
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D020552:Comet Assay; D003404:Creatinine / Q000097:blood; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D014508:Urea / Q000097:blood; D001613:beta 2-Microglobulin / Q000652:urine
chemical_list: D000975:Antioxidants; D009943:Organophosphorus Compounds; D010575:Pesticides; D001613:beta 2-Microglobulin; D008315:Malondialdehyde; D014508:Urea; D011794:Quercetin; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D000118:Acetylglucosaminidase; D005978:Glutathione
keywords: Acephate; NOAEL; dichlorvos; dimethoate; nephrotoxicity; oxidative stress; phorate; quercetin