title: Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol.
abstract: OBJECTIVE
  To evaluate the adhesion of human lens capsule cells on hydrophilic acrylic intraocular lenses (IOLs) coated with polyethylene glycol (PEG).
  
  
  METHODS
  Department of Ophthalmology, Faculty of Medicine, Universidade Estadual Paulista-Botucatu, São Paulo, Brazil.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Human anterior lens capsules obtained during cataract surgery were cultured and seeded (200 cells/IOLs) in triplicates on the surface of a copolymer comprising hydroxyethyl methacrylate, ethyl methacrylate, and methyl methacrylate IOLs (Loflex) treated or not treated with PEG. After 26 hours, the number of viable adherent cells was estimated by counting in a hemocytometer.
  
  
  RESULTS
  The coating of hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion (P < .05). Cells showing 2 distinct morphologic patterns-epithelial and dendritic-like-were observed during the in vitro establishment of the cultures. A tendency toward greater adhesion of dendritic-like cells was observed in untreated IOLs compared with treated IOLs (P = .095).
  
  
  CONCLUSIONS
  Coating hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion. This treatment might play a role in posterior capsule opacification prevention.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil. Electronic address: magdahata@yahoo.com.
authors.forename: Magda Massae Hata
authors.initials: MM
authors.lastname: Viveiros
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Ricardo Torres
authors.initials: RT
authors.lastname: Soares
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Michelle Sako
authors.initials: MS
authors.lastname: Omodei
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Cláudia Aparecida
authors.initials: CA
authors.lastname: Rainho
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Padovani
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Nilson
authors.initials: N
authors.lastname: Cruz
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Silvana Artioli
authors.initials: SA
authors.lastname: Schellini
authors.affiliation: From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
authors.forename: Antonio Carlos Lottelli
authors.initials: AC
authors.lastname: Rodrigues
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000166:cytology*; D007910:Lenses, Intraocular; D008858:Microscopy, Phase-Contrast; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011092:Polyethylene Glycols