title: Neuroprotective effects of 3-O-demethylswertipunicoside against MPTP-induced Parkinson's disease in vivo and its antioxidant properties in vitro.
abstract: 3-O-demethylswertipunicoside (3-ODS) has been reported to protect dopaminergic neurons against neurotoxicity induced by 1-methyl-4-phenylpyridinium (MPP(+)) in PC12 cells. Here, we investigate the neuroprotective effects in vivo and antioxidant activities in vitro of 3-ODS. In the 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-treated mouse model of Parkinson's disease (PD), 3-ODS dose-dependently improved motor coordination (as shown by rotarod test), increased the contents of dopamine (DA) and its metabolites in the striatum, and increased the number of tyrosine hydroxylase (TH)-positive neurons in the substantia nigra (SN). In addition, 3-ODS also increased the spine density in hippocampal CA1 neurons. In antioxidant assays, 3-ODS showed a strong capacity in scavenging hydroxyl radical, superoxide anion and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical in a concentration-dependent manner. Taken together, we conclude that 3-ODS attenuates the PD-related motor deficits mainly through its neuroprotective effects, growth-promoting effects on spine density, and its antioxidant activities.
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China; Department of Pharmacology, Dalian Medical University, Dalian 116044, PR China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Zhou
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Shen-Yu
authors.initials: SY
authors.lastname: Zhai
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Hui-Nan
authors.initials: HN
authors.lastname: Zhang
authors.affiliation: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Wang
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China. Electronic address: pxp2020@126.com.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology; D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001713:Biphenyl Compounds / Q000378:metabolism; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000737:chemistry / Q000627:therapeutic use*; D006624:Hippocampus / Q000187:drug effects; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D020267:MPTP Poisoning / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D010851:Picrates / Q000378:metabolism; D013131:Spine / Q000187:drug effects / Q000473:pathology; D013481:Superoxides / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D044004:Xanthones / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: C583860:3-O-demethylswertipunicoside; D001713:Biphenyl Compounds; D005960:Glucosides; D018696:Neuroprotective Agents; D010851:Picrates; D044004:Xanthones; D015102:3,4-Dihydroxyphenylacetic Acid; D013481:Superoxides; D017665:Hydroxyl Radical; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; C004931:1,1-diphenyl-2-picrylhydrazyl; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine
keywords: 1-methyl-4-phenyl-1, 2, 3; 3-O-demethylswertipunicoside; 6-tetrahydropyridine; Antioxidant activity; Growth-promoting effect; Neuroprotection; Parkinson׳s disease