title: Study on viability and chondrogenic differentiation of cryopreserved adipose tissue-derived mesenchymal stromal cells for future use in regenerative medicine.
abstract: Adipose-derived mesenchymal stromal cells are promising as a regenerative therapy tool for defective tissues in mesenchymal lineage, including fat, bone, cartilage, and blood vessels. In potential future clinical applications, adipose-derived stem cell cryopreservation is an essential fundamental technology. The aim of this study is to define an adequate protocol for the cryopreservation of adipose-derived mesenchymal stromal cells, by comparing various protocols so as to determine the effects of cryopreservation on viability and chondrogenic differentiation potential of adipose-derived stem cells upon freeze-thawing of AT-MSCs colonies cryopreserved with standard and modified protocols, using flow cytometry and confocal microscopy. The study concludes that adipose-derived mesenchymal stromal cells could be long-term cryopreserved without any loss of their proliferative or differentiation potential.
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Campus de Vegazana, University of León, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: González-Fernández
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Campus de Vegazana, University of León, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Pérez-Castrillo
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Campus de Vegazana, University of León, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Ordás-Fernández
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, Campus de Vegazana, University of León, Spain.
authors.forename: M E
authors.initials: ME
authors.lastname: López-González
authors.affiliation: Zootecnia Department, CECAV, University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.
authors.forename: B
authors.initials: B
authors.lastname: Colaço
authors.affiliation: Biomedicine Institute, Faculty of Veterinary Medicine, Campus de Vegazana, University of León, Spain. Electronic address: vega.villar@unileon.es.
authors.forename: V
authors.initials: V
authors.lastname: Villar-Suárez
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D002356:Cartilage / Q000166:cytology; D049109:Cell Proliferation; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D020219:Chondrogenesis; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D044968:Regenerative Medicine
chemical_list: D003451:Cryoprotective Agents
keywords: Adipose derived mesenchymal stromal cells; Chondrogenic differentiation; Cryopreservation