title: Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.
abstract: We describe a system for rapidly screening hundreds of nanoparticle samples using transmission electron microscopy (TEM). The system uses a liquid handling robot to place up to 96 individual samples onto a single standard TEM grid at separate locations. The grid is then transferred into the TEM and automated software is used to acquire multiscale images of each sample. The images are then analyzed to extract metrics on the size, shape, and morphology of the nanoparticles. The system has been used to characterize plasmonically active nanomaterials.
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.
authors.forename: Sean K
authors.initials: SK
authors.lastname: Mulligan
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Speir
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.|New York Structural Biology Center, New York, New York, 10027, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Razinkov
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.|New York Structural Biology Center, New York, New York, 10027, USA.
authors.forename: Anchi
authors.initials: A
authors.lastname: Cheng
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.
authors.forename: John
authors.initials: J
authors.lastname: Crum
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.
authors.forename: Tilak
authors.initials: T
authors.lastname: Jain
authors.affiliation: Scintillon Institute, San Diego, California, 92121, USA.
authors.forename: Erika
authors.initials: E
authors.lastname: Duggan
authors.affiliation: La Jolla Bioengineering Institute, San Diego, California, 92121, USA.
authors.forename: Er
authors.initials: E
authors.lastname: Liu
authors.affiliation: Scintillon Institute, San Diego, California, 92121, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Nolan
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.|New York Structural Biology Center, New York, New York, 10027, USA.
authors.forename: Bridget
authors.initials: B
authors.lastname: Carragher
authors.affiliation: The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.|New York Structural Biology Center, New York, New York, 10027, USA.
authors.forename: Clinton S
authors.initials: CS
authors.lastname: Potter
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D053758:Nanoparticles / Q000032:analysis*; D012371:Robotics / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*
keywords: TEM; automation; nanoparticles