title: Development and validation of an analytical method for the determination of 4-hexylresorcinol in food.
abstract: This study presents a method validation for extraction and quantitative analysis of 4-hexylresorcinol residues in shrimp and crab meat using HPLC-FLD. We were focused on the collaboratively analysis of each shrimp and crab meat samples, and developed LC-MS/MS method for the correct confirmation of the identity of compound. Validation parameters; selectivity, linearity, LOD, LOQ, accuracy, precision, and measurement of uncertainty were attained. The measurement of uncertainty was based on the precision study, data related to the performance of the analytical process and quantification of 4-hexylresorcinol. For HPLC-FLD analysis, the recoveries of 4-hexylresorcinol from spiked samples at levels of 0.2-10.0 ppm ranged from 92.54% to 97.67% with RSDs between 0.07% and 1.88%. According to these results, the method has been proven to be appropriate for extraction and determination of 4-hexylresorcinol, and can be used to maintain the safety of shrimp and crab products containing 4-hexylresorcinol residues.
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Young-Hyun
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: S-Food Guardians Co. Ltd, Seoul 136-701, Republic of Korea.
authors.forename: Seong-Ran
authors.initials: SR
authors.lastname: Gang
authors.affiliation: Food Additives and Packages Division, Ministry of Food and Drug Safety, Osong 363-951, Republic of Korea.
authors.forename: Ho-Soo
authors.initials: HS
authors.lastname: Lim
authors.affiliation: Food Additives and Packages Division, Ministry of Food and Drug Safety, Osong 363-951, Republic of Korea.
authors.forename: Meehye
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: loh99@kangwon.ac.kr.
authors.forename: Ok-Hwan
authors.initials: OH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006604:Hexylresorcinol / Q000737:chemistry*; D012758:Shellfish / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006604:Hexylresorcinol
keywords: 4-Hexylresorcinol; Crab; Fluorescence detection; HPLC; Shrimp