title: Kinetics of pesticide degradation by human fresh frozen plasma (FFP) in vitro.
abstract: There is an ongoing debate about the benefit of fresh frozen plasma (FFP) infusion in organophosphorus (OP) pesticide-poisoned patients. This prompted us to investigate the kinetics of OP pesticide degradation by FFP with an enzymatic assay in vitro. Degradation was rapid with shortest half-lives of 19.5s for chlorpyrifos-oxon, 6.3min for paraoxon-ethyl and 17.9min for dichlorvos. Heptenophos (78.0min), mevinphos (101.8min), profenofos (162.3min) and malaoxon (179.7min) showed half-lives of up to 3h. Substantial longer degradation half-lives of 69.7-80.8h were determined with chlorfenvinphos and bromfenvinphos. Methamidophos and omethoate showed no degradation by FFP indicated by half-lives similar to spontaneous hydrolysis. In conclusion, degradation by FFP depends on the particular OP pesticide and the used FFP batch.
authors.affiliation: Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: von der Wellen
authors.affiliation: Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Bierwisch
authors.affiliation: Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
authors.forename: Franz
authors.initials: F
authors.lastname: Worek
authors.affiliation: Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Thiermann
authors.affiliation: Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany. Electronic address: timowille@bundeswehr.org.
authors.forename: Timo
authors.initials: T
authors.lastname: Wille
mesh_terms: D043303:Aryldialkylphosphatase / Q000097:blood; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D006207:Half-Life; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D010575:Pesticides / Q000097:blood*; D010949:Plasma / Q000378:metabolism*
chemical_list: D010575:Pesticides; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
keywords: FFP; Fresh frozen plasma; Human cholinesterase; In vitro kinetics; Organophosphate; Pesticide