title: Selective extraction and analysis of catecholamines in rat blood microdialysate by polymeric ionic liquid-diphenylboric acid-packed capillary column and fast separation in high-performance liquid chromatography-electrochemical detector.
abstract: Concentration of blood catecholamines (CAs) is linked to a host of cardiovascular diseases, including hypertension and stenocardia. The matrix interferences and low concentration require tedious sample pretreatment methods before quantitative analysis by the gold standard method of high-performance liquid chromatography-electrochemical detector (HPLC-ECD). Solid phase extraction (SPE) has been widely used as the pretreatment technique. Here, a facile polymeric ionic liquid (PIL)-diphenylboric acid (DPBA)-packed capillary column was prepared to selectively extract dopamine (DA), noradrenaline (NE) and epinephrine (E) prior to their quantitative analysis by a fast separation in HPLC-ECD method, while microdialysis sampling method was applied to get the analysis sample. Parameters that influenced desorption efficiency, such as pH, salt concentration, acetonitrile content and wash time, were examined and optimized. The proposed method, combining microdialysis sampling technique, SPE and HPLC-ECD system, was successfully applied to detect CAs in rat blood microdialysate with high sensitivity and selectivity in small sample volumes (5-40μl) and a short analysis time (8min), yielding good reproducibility (RSD 6.5-7.7%) and spiked recovery (91-104.4%).
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China. Electronic address: gyshi@chem.ecnu.edu.cn.
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000097:blood; D055664:Electrochemical Techniques; D004837:Epinephrine / Q000097:blood; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids; D017551:Microdialysis; D009638:Norepinephrine / Q000097:blood; D011108:Polymers; D011137:Polystyrenes / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D001896:Boron Compounds; D002395:Catecholamines; D007093:Imidazoles; D052578:Ionic Liquids; D011108:Polymers; D011137:Polystyrenes; C000603191:poly(1-vinyl-3-propylphenyl imidazolium chloride-co-divinylbenzene); C035757:diphenylborate; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine
keywords: Catecholamines; PIL-DPBA-packed capillary column; Rat blood microdialysate; Solid phase extraction