title: [Effect of Cynaropicrin on 2,3,4,7,8-Pentachlorodibenzofuran-induced Wasting Syndrome and Oxidative Stress].
abstract: The effect of cynaropicrin that is the major component of an edible plant, artichoke (Cynara scolymus) on 2,3,4,7,8-pentachlorodibenzofuran (PenCDF)-induced toxicity in mice was studied. We evaluated the effect of cynaropicrin on the wasting syndrome and oxidative stress elicited by PenCDF. However, the PenCDF dose-response relationship on the wasting syndrome has been superficial. Therefore, we determined the dose which causes wasting syndrome in C57BL/6J mice, a responsive strain to dioxins. Since 2,3,7,8-tetrachlorodibenzo-p-dioxin (0.1 mg/kg, p.o.) induces hepatic ethoxyresorfin O-deethylase (EROD) activity in mice, we set the doses of PenCDF at 0.3, 1.0, 3.0, 5.0 and 10 mg/kg (once, p.o.) on the basis of its toxic-eqivalency factor (0.3). The wasting syndrome was evaluated by measuring the daily changes of body weight. Thiobarbituric acid-reactive substances were used as an index of oxidative stress. Of PenCDF doses examined, wasting syndrome and oxidative stress took place most markedly in 5 mg/kg. In disagreement with this, EROD activity which is the marker of the aryl hydrocarbon receptor-dependent induction of cytochrome P450 1a1 was elevated most abundantly at 0.3 mg/kg. Then, we examined the effect of cynaropicrin on the wasting syndrome and oxidative stress provoked by PenCDF at 5 mg/kg. However, this compound up to 20 mg/kg (p.o.) did not attenuate PenCDF-induced wasting syndrome. On the contray, PenCDF-induced oxidateive stress was suppressed by cynaropicrin at the highest dose (20 mg/kg), although EROD activity was increased rather than reduced by cynaropicrin at lower doses. Thus, it is suggested that cynaropicrin has an ability to reduce oxidative stress caused by PenCDF.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yamada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishii
authors.forename: Tomoki
authors.initials: T
authors.lastname: Takeda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kuroki
authors.forename: Chikage
authors.initials: C
authors.lastname: Mitoma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchi
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D007783:Lactones / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D012717:Sesquiterpenes / Q000494:pharmacology*; D019282:Wasting Syndrome / Q000139:chemically induced*
chemical_list: D001572:Benzofurans; D007783:Lactones; D012717:Sesquiterpenes; C075687:cynaropicrin; C038890:2,3,4,7,8-pentachlorodibenzofuran