title: Pr-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (α2βδε).
abstract: Three-finger toxins (3FTxs) are one of the major components in snake venoms. In this study, we isolated a cDNA encoding a short-chain 3FTx, Pr-SNTX, from Pseudechis rossignolii. The amino acid sequence of Pr-SNTX is nearly identical to that of its ortholog in Pseudechis australis. Pr-SNTX protein inhibited muscle-type (α2βδε), but not neuronal α7 nicotinic acetylcholine receptor (nAChR) activity.
authors.affiliation: a Biomedical Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: a Biomedical Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Kimoto
authors.affiliation: b College of Animal Science and Biotechnology , Zhejiang A & F University , Lin'An , China.
authors.forename: Xianyu
authors.initials: X
authors.lastname: Yang
authors.affiliation: c Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , Moscow , Russian Federation.
authors.forename: Sergey
authors.initials: S
authors.lastname: Filkin
authors.affiliation: c Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , Moscow , Russian Federation.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Utkin
authors.affiliation: a Biomedical Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.|d United Graduate School of Drug Discovery and Medical Information Sciences , Gifu University , Gifu , Japan.
authors.forename: Tai
authors.initials: T
authors.lastname: Kubo
authors.affiliation: a Biomedical Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inagaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004546:Elapid Venoms / Q000302:isolation & purification / Q000633:toxicity*; D017815:Elapidae / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009498:Neurotoxins / Q000302:isolation & purification / Q000633:toxicity*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D021122:Protein Subunits / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014982:Xenopus laevis
chemical_list: D018076:DNA, Complementary; D004546:Elapid Venoms; D009498:Neurotoxins; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D011994:Recombinant Proteins
keywords: Papuan pigmy mulga snake; nAChR; three-finger toxin; venom gland