title: In vivo second-harmonic generation and ex vivo coherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit.
abstract: Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work we used two different techniques that require no labeling: second harmonic generation (SHG) for collagen detection and coherent anti-Stokes Raman scattering (CARS) to assess lipid distribution in genetically obese murine skin. Obesity is one of the most serious public health problems due to its high and increasing prevalence and the associated risk of type 2 diabetes and cardiovascular diseases. Other than these diseases, nearly half of patients with diabetes mellitus suffer from dermatological complications such as delayed wound healing, foot ulcers and several other skin changes. In our experiment we investigated and followed the effects of obesity on dermal collagen alterations and adipocyte enlargement using a technique not reported in the literature so far. Our results indicate that the in vivo SHG and ex vivo CARS imaging technique might be an important tool for diagnosis of diabetes-related skin disorders in the near future.
authors.affiliation: Department of Dermatology, Venereology and Dermatooncology, Semmelweis University Hungary, Budapest, Hungary.|Department of Applied and Nonlinear Optics, Institute for Solid State Physics and Optics, Budapest, Hungary.
authors.forename: Dóra
authors.initials: D
authors.lastname: Haluszka
authors.affiliation: Department of Dermatology, Venereology and Dermatooncology, Semmelweis University Hungary, Budapest, Hungary.
authors.forename: Kende
authors.initials: K
authors.lastname: Lőrincz
authors.affiliation: MTA-SZTE Research Group for Cortical Microcircuits, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Molnár
authors.affiliation: MTA-SZTE Research Group for Cortical Microcircuits, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Tamás
authors.affiliation: Department of Applied and Nonlinear Optics, Institute for Solid State Physics and Optics, Budapest, Hungary.|R&D Ultrafast Lasers Ltd, Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Kolonics
authors.affiliation: Department of Applied and Nonlinear Optics, Institute for Solid State Physics and Optics, Budapest, Hungary.|R&D Ultrafast Lasers Ltd, Budapest, Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Szipőcs
authors.affiliation: Department of Dermatology, Venereology and Dermatooncology, Semmelweis University Hungary, Budapest, Hungary.
authors.forename: Sarolta
authors.initials: S
authors.lastname: Kárpáti
authors.affiliation: Department of Dermatology, Venereology and Dermatooncology, Semmelweis University Hungary, Budapest, Hungary.
authors.forename: Norbert M
authors.initials: NM
authors.lastname: Wikonkál
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis*; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008055:Lipids / Q000032:analysis*; D008820:Mice, Obese; D008853:Microscopy / Q000379:methods*; D009765:Obesity / Q000473:pathology*; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D013059:Spectrum Analysis, Raman
chemical_list: D008055:Lipids; D003094:Collagen
keywords: adipocyte; collagen; in vivo nonlinear microscopy; label-free imaging methods; obesity; skin