title: Effects of peripheral blood mononuclear cells on Haemonchus contortus larval motility in vitro.
abstract: The objective of this experiment was to determine effects of peripheral blood mononuclear cells (PBMC), derived from parasite-resistant St. Croix (STC) and parasite-susceptible Suffolk (SF) sheep, on motility of Haemonchus contortus L3stage larvae in vitro. Peripheral blood mononuclear cells were collected from 10 lambs of each breed, 5 naïve and 5 which had received a priming infection with H. contortus. Larval motility was quantified using a MIF Nikon Sweptfield microscope and NIS Elements AR software, and measurements included path length (PL) (μm), velocity (VEL) (μm/s) and acceleration (ACC) (μm/s2). After 18 h of incubation, PL and VEL were greatest in larvae cultured with SF-derived PMBC and were significantly different from all other groups (P < 0·01). No difference was observed in PL or VEL between larvae exposed to naïve or primed STC-derived PBMC and primed-SF PBMC. Differences in ACC were detected between larvae cultured with primed STC-derived PBMC (10·91 μm/s2) and naïve SF-derived PBMC (45·7 μm/s2) (P = 0·035). These data indicate an innate ability of STC-derived PBMC to severely inhibit larval motility.
authors.affiliation: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Holt
authors.affiliation: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Shepherd
authors.affiliation: Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV, 26506, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Ammer
authors.affiliation: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Bowdridge
keywords: 
  Haemonchus contortus
  ; 
  PBMC
  ; St. Croix; larval motility; parasite resistance