title: Odontoblast β-catenin signaling regulates fenestration of mouse Hertwig's epithelial root sheath.
abstract: The interaction between Hertwig's epithelial root sheath (HERS) and the adjacent mesenchyme is vitally important in mouse tooth root development. We previously generated odontoblast-specific Ctnnb1 (encoding β-catenin) deletion mice, and demonstrated that odontoblast β-catenin signaling regulates odontoblast proliferation and differentiation. However, the role of odontoblast β-catenin signaling in regulation of HERS behavior has not been fully investigated. Here, using the same odontoblast- specific Ctnnb1 deletion mice, we found that ablation of β-catenin signaling in odontoblasts led to aberrant HERS formation. Mechanistically, odontoblast-specific Ctnnb1 deletion resulted in elevated bone morphogenetic protein 7 (Bmp7) expression and reduced expression of noggin and follistatin, both of which encode extracellular inhibitors of BMPs. Furthermore, the levels of phosphorylated Smad1/5/8 were increased in HERS cells. In vitro tissue culture confirmed that BMP7 treatment disrupted the HERS structure. Taken together, we demonstrated that odontoblast β-catenin signaling may act through regulation of BMP signaling to maintain the integrity of HERS cells.
authors.affiliation: Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, 100081, China.|State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, 100071, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, 100071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
authors.affiliation: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, 100071, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, 100071, China.
authors.forename: JingTing
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, 100071, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, 100071, China. yogopop3@163.com.
authors.forename: Guan
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, 100081, China. litiejun22@vip.sina.com.
authors.forename: TieJun
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D004658:Enamel Organ / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition; D038681:Follistatin / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation; D005838:Genotype; D017403:In Situ Hybridization; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D014092:Tooth Root / Q000196:embryology* / Q000502:physiology*; D015854:Up-Regulation; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*; D000099176:Noggin Protein
chemical_list: D055419:Bone Morphogenetic Protein 7; D002352:Carrier Proteins; D038681:Follistatin; D051785:Smad Proteins; D051176:beta Catenin; D000099176:Noggin Protein; C495267:CTNNB1 protein, mouse; C525820:bmp7 protein, mouse
keywords: BMP signaling; Hertwig’s epithelial root sheath; mesenchyme-epithelium induction; β-catenin signaling