title: Detection and qualification of optimum antibacterial and cytotoxic activities of silver-doped bioactive glasses.
abstract: This study aims to detect the optimum antibacterial activity of silver-doped bioactive glasses (Ag-BGs) for prevention of post-transplant infections in tissue engineering. The results have shown that the Ag-BG samples had broad-spectrum antibacterial efficacy in an Ag concentration-dependent manner. The 2% Ag-BG had the highest effect during the first 10 min to 72 h. The minimum inhibitory concentration of 2% Ag-BG was estimated to be 2 mg/ml for Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) and 2.66 mg/ml for Staphylococcus aureus (S. aureus). A concentration of 0.5% Ag-BG repressed growth of E. coli after 1 h, but did not have any detectable antibacterial effect for longer periods. Evaluation of the effects of prepared Ag-BG on human osteoblast cells viability showed that 1 and 2% samples changed the cell proliferation rate in masses of more than 3.33 and 2 mg/ml, respectively. Moreover, in a typical manner, the release of Ag ions from the glass structure started immediately, continued steadily and affected bacterial growth when it reached its critical concentration in the medium. This systematic study can illustrate the optimum antibacterial property of the Ag-BG samples in masses of 3.33 and 2 mg/ml for 1 and 2% Ag, respectively, for prevention of post-transplant infections.
authors.affiliation: Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6354, Tehran, Iran.
authors.forename: Mazaher
authors.initials: M
authors.lastname: Gholipourmalekabadi
authors.affiliation: Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Karaj, Alborz, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Nezafati
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Hajibaki
authors.affiliation: Bioengineering Research Group,Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Mozafari
authors.affiliation: Faculty of Biomedical Engineering (Center of Excellence), Biomaterials Group, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Moztarzadeh
authors.affiliation: Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Karaj, Alborz, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Hesaraki
authors.affiliation: Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. samadikuchaksaraei@yahoo.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Samadikuchaksaraei
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005898:Glass / Q000737:chemistry; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D012834:Silver / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver