title: CPP-Based Delivery System for In Vivo Gene Delivery.
abstract: The method presents most important steps in estimating a CPP-mediated reporter gene delivery in a mouse model. The method is applicable for administrating noncovalent CPP/pDNA complexes via i.v. injection and for analysis of luciferase levels in tissue homogenates. This method could be extended to analyze the delivery of different nucleic acid cargos with other types of delivery vectors. First, a simple method is presented for assessing the stability of complexes in blood after i.v. administration, based on quantitation of a fluorescently labeled nucleic acid. Secondly, a protocol is presented for assessing and analyzing luciferase levels in mouse organs.
authors.affiliation: Laboratory of Molecular Biotechnology, Institute of Technology, Tartu University, Nooruse 1/517, Tartu, 50411, Estonia, kaido.kurrikoff@ut.ee.
authors.forename: Kaido
authors.initials: K
authors.lastname: Kurrikoff
authors.forename: Kadi-Liis
authors.initials: KL
authors.lastname: Veiman
authors.forename: Ülo
authors.initials: Ü
authors.lastname: Langel
mesh_terms: D000818:Animals; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D005470:Fluorometry; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D049408:Luminescent Agents / Q000032:analysis / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics
chemical_list: D057846:Cell-Penetrating Peptides; D049408:Luminescent Agents; D004247:DNA; D008156:Luciferases