title: Differential peptidomics assessment of strain and age differences in mice in response to acute cocaine administration.
abstract: Neurochemical differences in the hypothalamic-pituitary axis between individuals and between ages may contribute to differential susceptibility to cocaine abuse. This study measured peptide levels in the pituitary gland (Pit) and lateral hypothalamus (LH) in adolescent (age 30 days) and adult (age 65 days) mice from four standard inbred strains, FVB/NJ, DBA/2J, C57BL/6J, and BALB/cByJ, which have previously been characterized for acute locomotor responses to cocaine. Individual peptide profiles were analyzed using mass spectrometric profiling and principal component analysis. Sequences of assigned peptides were verified by tandem mass spectrometry. Principal component analysis classified all strains according to their distinct peptide profiles in Pit samples from adolescent mice, but not adults. Select pro-opiomelanocortin-derived peptides were significantly higher in adolescent BALB/cByJ and DBA/2J mice than in FVB/NJ or C57BL/6J mice. A subset of peptides in the LH, but not in the Pit, was altered by cocaine in adolescents. A 15 mg/kg dose of cocaine induced greater peptide alterations than a 30 mg/kg dose, particularly in FVB/NJ animals, with larger differences in adolescents than adults. Neuropeptides in the LH affected by acute cocaine administration included pro-opiomelanocortin-, myelin basic protein-, and glutamate transporter-derived peptides. The observed peptide differences could contribute to differential behavioral sensitivity to cocaine among strains and ages. Peptides were measured using mass spectrometry (MALDI-TOF) in individual lateral hypothalamus and pituitary samples from four strains and two ages of inbred mice in response to acute cocaine administration. Principal component analyses (PCA) classified the strains according to their peptide profiles from adolescent mice, and a subset of peptides in the lateral hypothalamus was altered by cocaine in adolescents.
authors.affiliation: Department of Chemistry, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Romanova
authors.affiliation: Department of Chemistry, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Stanislav S
authors.initials: SS
authors.lastname: Rubakhin
authors.affiliation: Department of Psychology, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Ossyra
authors.affiliation: Department of Psychology, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Zombeck
authors.affiliation: Department of Psychology, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Nosek
authors.affiliation: Department of Chemistry, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Jonathan V
authors.initials: JV
authors.lastname: Sweedler
authors.affiliation: Department of Psychology, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Justin S
authors.initials: JS
authors.lastname: Rhodes
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002853:Chromatography, Liquid; D003042:Cocaine / Q000008:administration & dosage*; D018765:Dopamine Uptake Inhibitors / Q000008:administration & dosage*; D007026:Hypothalamic Area, Lateral / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D010902:Pituitary Gland / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018765:Dopamine Uptake Inhibitors; D010455:Peptides; D003042:Cocaine
keywords: adolescent; cocaine; label-free quantitation; matrix-assisted laser desorption ionization MS; peptidomics; principal component analysis