title: Improved conversion of cinnamaldehyde derivatives to diol compounds via a pyruvate decarboxylase-dependent mechanism in budding yeast.
abstract: Cinnamaldehyde is stereospecifically converted to (2S,3R) 5-phenylpent-4-ene-2,3-diol, an important starting material for the synthesis of biologically active compounds, by the budding yeast Saccharomyces cerevisiae. Immobilization of the yeast in calcium alginate capsules suppressed the formation of by-products and increased accumulation of the diol compounds. The mechanism of cinnamaldehyde conversion was investigated by using recombinant strains of Escherichia coli and S. cerevisiae carrying the pyruvate decarboxylase gene PDC1. As a result, condensation of the substrate with acetaldehyde was enhanced by PDC and flow to the diol product was altered.
authors.affiliation: National Institute of Technology, Gunma College, 580 Toriba-machi, Maebashi, Gunma, 371-8530, Japan. Electronic address: miyako@nat.gunma-ct.ac.jp.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Miyakoshi
authors.affiliation: National Institute of Technology, Gunma College, 580 Toriba-machi, Maebashi, Gunma, 371-8530, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Negishi
authors.affiliation: National Institute of Technology, Gunma College, 580 Toriba-machi, Maebashi, Gunma, 371-8530, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sekiya
authors.affiliation: National Institute of Technology, Gunma College, 580 Toriba-machi, Maebashi, Gunma, 371-8530, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakajima
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D000171:Acrolein / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000438:Alcohols / Q000737:chemistry / Q000378:metabolism*; D000464:Alginates; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D000438:Alcohols; D000464:Alginates; D006603:Hexuronic Acids; D000171:Acrolein; D020723:Glucuronic Acid; D011767:Pyruvate Decarboxylase; D000079:Acetaldehyde; C012843:cinnamaldehyde
keywords: Bioconversion; Cinnamaldehyde; Diol; Immobilized yeast; Pyruvate decarboxylase; Saccharomyces cerevisiae