title: Human Adipose-Derived Mesenchymal Stem Cells Promote Liver Regeneration.
abstract: OBJECTIVE
  Liver regeneration (LR) is of crucial importance to patients with acute liver failure, those undergoing live donor liver transplantations or extended liver resections. Effective treatment strategies aimed at accelerating liver regeneration could offer major benefits in these patients. Due to easy accessibility, human adipose-derived mesenchymal stem cells (HADMSC) are an attractive source for regenerative medicine. Herein, we investigated the effect of HADMSC on LR in a murine model. We hypothesized that HADMSC will promote LR.
  
  
  METHODS
  Mice were subjected to CCl4-induced acute liver failure (ALF). Animals in the experimental arm were treated with HADMSC prior to CCl4-induced ALF. Liver injury was evaluated using serum levels of alanine aminotransferase (ALT), serum interleukin-6 (IL-6), and histopathology. Liver samples were stained for a specific marker of regeneration, proliferating cell nuclear antigen (PCNA).
  
  
  RESULTS
  Histology, serum IL-6, and ALT release revealed that HADMSC treatment attenuated liver injury compared with control animals. In addition, animals treated with HADMSC were observed to have improved survival and increased number of PCNA positive cells on histology when compared with controls.
  
  
  CONCLUSIONS
  HADMSCs represent a potential therapeutic strategy to promote liver regeneration.
authors.affiliation: a 1 Brown University , Providence, Rhode Island, USA.
authors.forename: Reza
authors.initials: R
authors.lastname: Saidi
authors.affiliation: b 2 UMass , Worcester, Massachusetts, USA.
authors.forename: R
authors.initials: R
authors.lastname: Rajeshkumar
authors.affiliation: b 2 UMass , Worcester, Massachusetts, USA.
authors.forename: A
authors.initials: A
authors.lastname: Shariftabrizi
authors.affiliation: a 1 Brown University , Providence, Rhode Island, USA.
authors.forename: A
authors.initials: A
authors.lastname: Zimmerman
authors.affiliation: b 2 UMass , Worcester, Massachusetts, USA.
authors.forename: O
authors.initials: O
authors.lastname: Walter
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000473:pathology / Q000628:therapy; D064593:Heterografts; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008099:Liver / Q000473:pathology; D008115:Liver Regeneration; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D015850:Interleukin-6; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase
keywords: acute liver failure; liver regeneration; liver surgery; liver transplantation; mesenchymal stem cells; stem cells