title: Evaluation of the modified HTK solution in pancreas transplantation-An experimental model.
abstract: BACKGROUND
  One of the great challenges in pancreas transplantation is the ischemia reperfusion injury. It is mentioned that free oxygen and/or nitrogen radicals play a prominent role in this phase. To minimize this problem, a modified histidine-tryptophan-ketoglutarate (HTK) solution that contains modified antioxidants has been developed. Our aim was to evaluate this solution in improving the viability of the pancreas in comparison with standard HTK and University of Wisconsin (UW) solutions in a porcine model of pancreas transplantation.
  
  
  METHODS
  Twenty-three Landrace pigs were divided into three identical groups. After a 10-hour preservation time at 4°C, the pancreas was implanted in the organs of the recipients in a standardized manner. Serum parameters were assessed prior to and after implantation on the 1(st) postoperative day, 3(rd) postoperative day, and 7(th) postoperative day. Furthermore, three biopsies were taken: prior to and after reperfusion, and on Day 7 to assess the grafts.
  
  
  RESULTS
  An analysis of serum glucose among the three groups showed no significant differences. Evaluation of the insulin levels showed no significant difference between the modified and standard HTK groups; however, differences between HTK and UW were significant (p = 0.004 in favor of UW solutions). The histopathological results showed a trend of a higher grade of rejection of pancreas tissue in the UW group compared to both HTK groups.
  
  
  CONCLUSIONS
  The modified HTK solution could preserve the pancreas for the preservation of the graft with similar results to those observed for standard solutions without any significant difference. The trend showed that the pathological finding in the UW group was not as good as that in the modified HTK and standard HTK groups.
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Majid
authors.initials: M
authors.lastname: Esmaeilzadeh
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Fonouni
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Golriz
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Ali
authors.initials: A
authors.lastname: Majlesara
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Gani
authors.initials: G
authors.lastname: Kuttymuratov
authors.affiliation: Department of Pathology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Bergmann
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Parvin
authors.initials: P
authors.lastname: Jarahian
authors.affiliation: Research Development Center, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khazaeipour
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Alireza
authors.initials: A
authors.lastname: Faridar
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Morva Tahmasbi
authors.initials: MT
authors.lastname: Rad
authors.affiliation: Department of Pathology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Longerich
authors.affiliation: Department of Experimental Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Martha M
authors.initials: MM
authors.lastname: Gebhard
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany. Electronic address: arianeb_mehrabi@med.uni-heidelberg.de.
authors.forename: Arianeb
authors.initials: A
authors.lastname: Mehrabi
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D005947:Glucose; D005978:Glutathione; D007328:Insulin; D008353:Mannitol; D019188:Organ Preservation Solutions; D016035:Pancreas Transplantation / Q000379:methods*; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D013552:Swine
chemical_list: C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose
keywords: University of Wisconsin; histidine tryptophan ketoglutarate; ischemia reperfusion injury; pancreas transplantation