title: Effects of substrate concentration on methane potential and degradation kinetics in batch anaerobic digestion.
abstract: In this study, two experiments were conducted to evaluate the impact of substrate concentrations on methane potential and degradation kinetics of substrate. The biochemical methane potential (BMP) tests in Experiment I were performed at a constant inoculum to substrate ratio (ISR), whereas, different ISRs were applied in Experiment II. Results obtained from Experiment I revealed that methane potential of substrate increased at a saturating trend with higher substrate concentrations, and could differ by up to 30% between the lowest and highest investigated concentrations. The results of Experiment II verified the results of Experiment I, and further showed that this trend also occurs when the substrate concentration is regulated with ISRs. In contrast, substrate concentration had no significant impact on the degradation kinetics. It was concluded that dilutions should be avoided when the substrate concentration is lower than 10 g VS/L in order to avoid underestimations of methane potential from BMP test.
authors.affiliation: Department of Biotechnology, Lund University, Getingevägen 60, SE-221 00 Lund, Sweden. Electronic address: bing.wang@biotek.lu.se.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Lund University, Getingevägen 60, SE-221 00 Lund, Sweden.
authors.forename: Sten
authors.initials: S
authors.lastname: Strömberg
authors.affiliation: Department of Biotechnology, Lund University, Getingevägen 60, SE-221 00 Lund, Sweden.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Biotechnology, Lund University, Getingevägen 60, SE-221 00 Lund, Sweden.
authors.forename: Ivo Achu
authors.initials: IA
authors.lastname: Nges
authors.affiliation: Bioprocess Control Sweden AB, Scheelevägen 22, SE-223 63 Lund, Sweden.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Nistor
authors.affiliation: Biogas Institute of Ministry of Agriculture, 610041 Chengdu, China.
authors.forename: Liangwei
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Biotechnology, Lund University, Getingevägen 60, SE-221 00 Lund, Sweden; Bioprocess Control Sweden AB, Scheelevägen 22, SE-223 63 Lund, Sweden.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000468:Alkalies / Q000494:pharmacology; D000693:Anaerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques / Q000379:methods*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002482:Cellulose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008697:Methane / Q000378:metabolism*
chemical_list: D000468:Alkalies; D002482:Cellulose; D008697:Methane
keywords: Anaerobic digestion; Biochemical methane potential; Kinetics; Substrate concentration; Substrate load