title: Seasonal Variation on Microbial Community and Methane Production during Anaerobic Digestion of Cattle Manure in Brazil.
abstract: Anaerobic digestion is an alternative method for the treatment of animal manure and wastewater. The anaerobic bioconversion of biomass requires a multi-step biological process, including microorganisms with distinct roles. The diversity and composition of microbial structure in pilot-scale anaerobic digestion operating at ambient temperature in Brazil were studied. Influence of the seasonal and temporal patterns on bacterial and archaeal communities were assessed by studying the variations in density, dynamic and diversity and structure. The average daily biogas produced in the summer and winter months was 18.7 and 16 L day(-1), respectively, and there was no difference in the average methane yield. Quantitative PCR analysis revealed that no differences in abundances and dynamics were found for bacterial communities and the total number of Archaea in different seasons. Analysis of bacterial clone libraries revealed a predominance of Firmicutes (54.5 %/summer and 46.7 %/winter) and Bacteroidetes (31.4 %/summer and 44.4 %/winter). Within the Archaea, the phylum Euryarchaeota was predominant in both digesters. Phylogenetic distribution showed changes in percentage between the phyla identified, but no alterations were recorded in the quality and amount of produced methane or community dynamics. The results may suggest that redundancy of microbial groups may have occurred, pointing to a more complex microbial community in the ecosystem related to this ambient temperature system.
authors.affiliation: Laboratory of Bacterial Physiology and Molecular Genetics, Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36.036-900, Juiz de Fora, Minais Gerais, Brazil.|INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France.
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Resende
authors.affiliation: INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
authors.affiliation: INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Bonnafous
authors.affiliation: EMBRAPA Brazilian Agricultural Research Corporation, Secretariat for International Relations, Headquarters, Brasilia, Brazil.
authors.forename: Pedro Braga
authors.initials: PB
authors.lastname: Arcuri
authors.affiliation: Laboratory of Bacterial Physiology and Molecular Genetics, Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36.036-900, Juiz de Fora, Minais Gerais, Brazil.
authors.forename: Vânia Lúcia
authors.initials: VL
authors.lastname: Silva
authors.affiliation: EMBRAPA Dairy Cattle - Brazilian Agricultural Research Corporation, Juiz de Fora, Brazil.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Otenio
authors.affiliation: Laboratory of Bacterial Physiology and Molecular Genetics, Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36.036-900, Juiz de Fora, Minais Gerais, Brazil. claudio.diniz@ufjf.edu.br.
authors.forename: Cláudio Galuppo
authors.initials: CG
authors.lastname: Diniz
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D044822:Biodiversity; D001938:Brazil; D002417:Cattle; D008372:Manure / Q000032:analysis / Q000382:microbiology*; D008697:Methane / Q000032:analysis / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D008372:Manure; D008697:Methane
keywords: Anaerobic digestion; Archaea; Bacteria; Phylogenetic diversity; Temperature variation