title: MMP1 gene expression enhances myoblast migration and engraftment following implanting into mdx/SCID mice.
abstract: Myoblast transplantation (MT) is a method to introduce healthy genes into abnormal skeletal muscle. It has been considered as a therapeutic modality in the last few decades for diseases such as Duchenne Muscular Dystrophy (DMD). However, challenges including cell death and poor graft engraftment have limited its application. The current experiment utilizes MMP1 gene transfer to improve the efficacy of myoblast transplantation into the diseased dystrophic skeletal muscle of mdx mice. Our results indicated that MMP1 expression can promote myogenic differentiation and fusion capacities, increase migration of MMP1 expressing myoblasts in vitro, as well as improve engraftment of dystrophin positive myofibers in vivo. Taken together, our observation suggests that the addition of MMP1 can overcome limitations in MT and improve its clinical efficacy.
authors.affiliation: a Department of Pediatric Surgery ; University of Texas Medical School at Houston ; Houston , TX USA.
authors.forename: Haiying
authors.initials: H
authors.lastname: Pan
authors.forename: Kinga
authors.initials: K
authors.lastname: Vojnits
authors.forename: Thomas T
authors.initials: TT
authors.lastname: Liu
authors.forename: Fanwei
authors.initials: F
authors.lastname: Meng
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Yigang
authors.initials: Y
authors.lastname: Wang
authors.forename: Johnny
authors.initials: J
authors.lastname: Huard
authors.forename: Charles S
authors.initials: CS
authors.lastname: Cox
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Lally
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D016513:Mice, SCID; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism*
chemical_list: D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse
keywords: differentiation; duchenne muscular dystrophy (DMD); matrix metalloproteinase (MMP); mdx mice; migration; myoblast transplantation; skeletal muscle