title: Microfluidic Production of Alginate Hydrogel Particles for Antibody Encapsulation and Release.
abstract: Owing to their biocompatibility and reduced side effects, natural polymers represent an attractive choice for producing drug delivery systems. Despite few successful examples, however, the production of monodisperse biopolymer-based particles is often hindered by high viscosity of polymer fluids. In this work, we present a microfluidic approach for production of alginate-based particles carrying encapsulated antibodies. We use a triple-flow micro-device to induce hydrogel formation inside droplets before their collection off-chip. The fast mixing and gelation process produced alginate particles with a unique biconcave shape and dimensions of the mammalian cells. We show slow and fast dissolution of particles in different buffers and evaluate antibody release over time.
authors.affiliation: Vilnius University Institute of Biotechnology, Vilnius LT-02241, Lithuania. linas.mazutis@bti.vu.lt.|Harvard University, School of Engineering and Applied, Cambridge MA 02138, USA. linas.mazutis@bti.vu.lt.
authors.forename: Linas
authors.initials: L
authors.lastname: Mazutis
authors.affiliation: Vilnius University Institute of Biotechnology, Vilnius LT-02241, Lithuania.
authors.forename: Remigijus
authors.initials: R
authors.lastname: Vasiliauskas
authors.affiliation: Harvard University, School of Engineering and Applied, Cambridge MA 02138, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics; D020723:Glucuronic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D006603:Hexuronic Acids / Q000737:chemistry / Q000493:pharmacokinetics; D020100:Hydrogels / Q000737:chemistry / Q000493:pharmacokinetics; D055767:Immobilized Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D007074:Immunoglobulin G / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*
chemical_list: D000464:Alginates; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D020100:Hydrogels; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D020723:Glucuronic Acid
keywords: alginate particles; antibody; droplet microfluidics; drug delivery system