title: Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice.
abstract: The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.
authors.affiliation: Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics and PolicySchool of PharmacyUniversity of Southern California1985 Zonal Avenue, Los Angeles, CA, 90033, United States. Electronic address: asatryan@usc.edu.
authors.forename: Liana
authors.initials: L
authors.lastname: Asatryan
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90033, United States.
authors.forename: Sheraz
authors.initials: S
authors.lastname: Khoja
authors.affiliation: Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics and PolicySchool of PharmacyUniversity of Southern California1985 Zonal Avenue, Los Angeles, CA, 90033, United States.
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Rodgers
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90033, United States.
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Alkana
authors.affiliation: Southern California Research Center for Alcoholic Liver and Pancreatic Disease and Cirrhosis, Keck School of Medicine, University of Southern California, 1333 San Pablo Street, Los Angeles, CA 90033, United States.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tsukamoto
authors.affiliation: Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics and PolicySchool of PharmacyUniversity of Southern California1985 Zonal Avenue, Los Angeles, CA, 90033, United States.
authors.forename: Daryl L
authors.initials: DL
authors.lastname: Davies
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002452:Cell Count / Q000379:methods; D059305:Diet, High-Fat / Q000009:adverse effects*; D000431:Ethanol / Q000008:administration & dosage* / Q000633:toxicity; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D058486:Receptors, Purinergic P2X7 / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D018836:Inflammation Mediators; D058486:Receptors, Purinergic P2X7; D000431:Ethanol
keywords: Astrocytes; High fat diet; Intragastric ethanol feeding; Microglia; Neuroinflammation and neuronal loss; Pro-inflammatory cytokines; Purinergic P2X7 receptors