title: Constitutive Expression of Rice MicroRNA528 Alters Plant Development and Enhances Tolerance to Salinity Stress and Nitrogen Starvation in Creeping Bentgrass.
abstract: MicroRNA528 (miR528) is a conserved monocot-specific small RNA that has the potential of mediating multiple stress responses. So far, however, experimental functional studies of miR528 are lacking. Here, we report that overexpression of a rice (Oryza sativa) miR528 (Osa-miR528) in transgenic creeping bentgrass (Agrostis stolonifera) alters plant development and improves plant salt stress and nitrogen (N) deficiency tolerance. Morphologically, miR528-overexpressing transgenic plants display shortened internodes, increased tiller number, and upright growth. Improved salt stress resistance is associated with increased water retention, cell membrane integrity, chlorophyll content, capacity for maintaining potassium homeostasis, CATALASE activity, and reduced ASCORBIC ACID OXIDASE (AAO) activity; while enhanced tolerance to N deficiency is associated with increased biomass, total N accumulation and chlorophyll synthesis, nitrite reductase activity, and reduced AAO activity. In addition, AsAAO and COPPER ION BINDING PROTEIN1 are identified as two putative targets of miR528 in creeping bentgrass. Both of them respond to salinity and N starvation and are significantly down-regulated in miR528-overexpressing transgenics. Our data establish a key role that miR528 plays in modulating plant growth and development and in the plant response to salinity and N deficiency and indicate the potential of manipulating miR528 in improving plant abiotic stress resistance.
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634.
authors.forename: Shuangrong
authors.identifier: http://orcid.org/0000-0002-2546-689X
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634.
authors.forename: Zhigang
authors.identifier: http://orcid.org/0000-0003-3823-9845
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634.
authors.forename: Dayong
authors.identifier: http://orcid.org/0000-0003-1573-4747
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634.
authors.forename: Ning
authors.identifier: http://orcid.org/0000-0003-1484-7010
authors.initials: N
authors.lastname: Yuan
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634.
authors.forename: Qian
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634 hluo@clemson.edu.
authors.forename: Hong
authors.identifier: http://orcid.org/0000-0001-9223-1786
authors.initials: H
authors.lastname: Luo
mesh_terms: D031703:Agrostis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D042931:Aldehyde Oxidase / Q000378:metabolism; D001483:Base Sequence; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D055864:Droughts; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000172:deficiency*; D012275:Oryza / Q000235:genetics*; D063245:Plant Development / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D054712:Salinity; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D014867:Water
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014867:Water; D002734:Chlorophyll; D012965:Sodium Chloride; D011392:Proline; D012964:Sodium; D042931:Aldehyde Oxidase; D009584:Nitrogen; D011188:Potassium