title: High Voltage Electrochemiluminescence (ECL) as a New Method for Detection of PAH During Screening for PAH-Degrading Microbial Consortia.
abstract: The search for new bacterial consortia capable of removing PAH from the environment is associated with the need to employ novel, simple, and economically efficient detection methods. A fluorimetric method (FL) as well as high voltage electrochemiluminescence (ECL) on a modified surface of an aluminum electrode were used in order to determine the changes in the concentrations of PAH in the studied aqueous solutions. The ECL signal (the spectrum and emission intensity for a given wavelength) was determined with the use of an apparatus operating in single photon counting mode. The dependency of ECL and FL intensity on the concentration of naphthalene, phenanthrene, and pyrene was linear in the studied concentration range. The biodegradation kinetics of the particular PAH compounds was determined on the basis of the obtained spectroscopic determinations. It has been established that the half-life of naphthalene, phenanthrene, and pyrene at initial concentrations of 50 mg/l (beyond the solubility limit) reached 41, 75, and 130 h, accordingly. Additionally, the possibility of using ECL for rapid determination of the soluble fraction of PAH directly in the aqueous medium has been confirmed. Metagenomic analysis of the gene encoding 16S rRNA was conducted on the basis of V4 hypervariable region of the 16S rRNA gene and allowed to identify 198 species of bacteria that create the S4consortium. The consortium was dominated byGammaproteobacteria(78.82 %),Flavobacteria(9.25 %),Betaproteobacteria(7.68 %),Sphingobacteria(3.76 %),Alphaproteobacteria(0.42 %),Clostridia(0.04 %), andBacilli(0.03 %).
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Staninska
authors.affiliation: Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
authors.forename: Zuzanna
authors.initials: Z
authors.lastname: Szczepaniak
authors.affiliation: Department of Rare Earth, Faculty of Chemistry, Adam Mickiewicz University, ul. Umultowska 89b, 61-614 Poznań, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Staninski
authors.affiliation: Institute of Forensic Genetics, Al. Mickiewicza 3/4, 85-071 Bydgoszcz, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Czarny
authors.affiliation: Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piotrowska-Cyplik
authors.affiliation: Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Nowak
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Marecik
authors.affiliation: Institute of Chemical Technology and Engineering, Poznań University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Chrzanowski
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Cyplik
keywords: Biodegradation; High voltage electrochemiluminescence (ECL); Metagenomics analysis; PAH