title: [Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro].
abstract: OBJECTIVE
  To investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and osteopontin systhesis in vitro and explore the mechanisms.
  
  
  METHODS
  HASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting.
  
  
  RESULTS
  Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01).
  
  
  CONCLUSIONS
  Anti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.
authors.affiliation: Second Department of Internal Medicine, Third People's Hospital of Shenzhen, Shenzhen 518112, China.E-mail: drcpf@sohu.com.
authors.forename: Peifen
authors.initials: P
authors.lastname: Chen
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Qiu
authors.forename: Guohua
authors.initials: G
authors.lastname: Huang
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhang
authors.forename: Wujian
authors.initials: W
authors.lastname: Peng
authors.forename: Hui
authors.initials: H
authors.lastname: Ceng
authors.forename: Wenyan
authors.initials: W
authors.lastname: Lai
mesh_terms: D056151:Airway Remodeling; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D053495:Osteopontin / Q000096:biosynthesis*; D012137:Respiratory System / Q000166:cytology
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D053495:Osteopontin