title: Gelucire-Based Nanoparticles for Curcumin Targeting to Oral Mucosa: Preparation, Characterization, and Antimicrobial Activity Assessment.
abstract: The purpose of the study was to prepare and characterize curcumin (Cur) solid lipid nanoparticles (CurSLN) with a high-loading capacity and chemical stability for the treatment of oral mucosal infection. CurSLN were formulated using different lipids, namely, Gelucire 39/01, Gelucire 50/13, Precirol, Compritol, and poloxamer 407 as a surfactant. Formulae were evaluated for their entrapment efficiency, particle size, and ex vivo mucoadhesion test. Microbiological evaluation was carried out on six microorganisms, five of which are the most commonly affecting oral cavity in terms of determination of minimum inhibitory concentration (MIC), and minimum bactericidal concentration. Transmission electron microscopy was conducted for ultrathin section for Candida albicans-treated with formulated Cur. The results showed high entrapment efficiency and stability enhancement for Cur powder. Significant amount of Cur was retained onto the mucosal tissue indicating preferential mucosal uptake. CurSLN showed higher antimicrobial activity as compared with Cur raw material and chemically stabilized Cur where it showed MIC (0.185, 0.09375, 0.75, 3, 1.5, and 0.1875 mg/mL) against Staphylococcus aureus, Streptococcus mutans, Viridansstrept, Escherichia coli, Lactobacillus acidophilus, and Candida albicans, respectively. The prepared lipid nanoparticles maintained Cur chemical stability and microbiological activity. The lack of local antimicrobial therapeutics with minimum side effects augments the importance of studying natural products for this purpose.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt. Electronic address: hebahazzah@yahoo.com.
authors.forename: Heba A
authors.initials: HA
authors.lastname: Hazzah
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt.
authors.forename: Ragwa M
authors.initials: RM
authors.lastname: Farid
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
authors.forename: Maha M A
authors.initials: MMA
authors.lastname: Nasra
authors.affiliation: Department of Microbiology, High Institute of Public Health, Alexandria University, Alexandria, Egypt.
authors.forename: Walaa A
authors.initials: WA
authors.lastname: Hazzah
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt.
authors.forename: Magda A
authors.initials: MA
authors.lastname: El-Massik
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
authors.forename: Ossama Y
authors.initials: OY
authors.lastname: Abdallah
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D002645:Chickens; D003474:Curcumin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects; D008055:Lipids / Q000737:chemistry*; D009061:Mouth Mucosa / Q000378:metabolism* / Q000382:microbiology; D009181:Mycoses / Q000188:drug therapy; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D004337:Drug Carriers; D008055:Lipids; D003474:Curcumin
keywords: antimicrobial activity; buccal; cell disintegration; curcumin; drug delivery system; lipid; mucosal delivery; nanoparticles