title: Calorie Restriction Suppresses Age-Dependent Hippocampal Transcriptional Signatures.
abstract: Calorie restriction (CR) enhances longevity and mitigates aging phenotypes in numerous species. Physiological responses to CR are cell-type specific and variable throughout the lifespan. However, the mosaic of molecular changes responsible for CR benefits remains unclear, particularly in brain regions susceptible to deterioration during aging. We examined the influence of long-term CR on the CA1 hippocampal region, a key learning and memory brain area that is vulnerable to age-related pathologies, such as Alzheimer's disease (AD). Through mRNA sequencing and NanoString nCounter analysis, we demonstrate that one year of CR feeding suppresses age-dependent signatures of 882 genes functionally associated with synaptic transmission-related pathways, including calcium signaling, long-term potentiation (LTP), and Creb signaling in wild-type mice. By comparing the influence of CR on hippocampal CA1 region transcriptional profiles at younger-adult (5 months, 2.5 months of feeding) and older-adult (15 months, 12.5 months of feeding) timepoints, we identify conserved upregulation of proteome quality control and calcium buffering genes, including heat shock 70 kDa protein 1b (Hspa1b) and heat shock 70 kDa protein 5 (Hspa5), protein disulfide isomerase family A member 4 (Pdia4) and protein disulfide isomerase family A member 6 (Pdia6), and calreticulin (Calr). Expression levels of putative neuroprotective factors, klotho (Kl) and transthyretin (Ttr), are also elevated by CR in adulthood, although the global CR-specific expression profiles at younger and older timepoints are highly divergent. At a previously unachieved resolution, our results demonstrate conserved activation of neuroprotective gene signatures and broad CR-suppression of age-dependent hippocampal CA1 region expression changes, indicating that CR functionally maintains a more youthful transcriptional state within the hippocampal CA1 sector.
authors.affiliation: Cell and Molecular Biology Program, New York University Langone Medical Center, New York, New York, United States of America; Center for Dementa Research, Nathan Kline Institute, Orangeburg, New York, United States of America.
authors.forename: Marissa J
authors.initials: MJ
authors.lastname: Schafer
authors.affiliation: Genome Technology Center, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Igor
authors.initials: I
authors.lastname: Dolgalev
authors.affiliation: Center for Dementa Research, Nathan Kline Institute, Orangeburg, New York, United States of America; Department of Psychiatry, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Alldred
authors.affiliation: Genome Technology Center, New York University Langone Medical Center, New York, New York, United States of America; Department of Pathology, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Adriana
authors.initials: A
authors.lastname: Heguy
authors.affiliation: Cell and Molecular Biology Program, New York University Langone Medical Center, New York, New York, United States of America; Center for Dementa Research, Nathan Kline Institute, Orangeburg, New York, United States of America; Department of Psychiatry, New York University Langone Medical Center, New York, New York, United States of America; Department of Neuroscience and Physiology, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Ginsberg
mesh_terms: D000375:Aging / Q000235:genetics* / Q000502:physiology; D000818:Animals; D001835:Body Weight / Q000235:genetics; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000378:metabolism*; D031204:Caloric Restriction; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D020869:Gene Expression Profiling; D008136:Longevity / Q000235:genetics; D051379:Mice; D000066829:Neuroprotection / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717248:Hspa5 protein, mouse; D012333:RNA, Messenger