title: [Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification].
abstract: A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N S
authors.initials: NS
authors.lastname: Bukharina
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Yu D
authors.initials: YD
authors.lastname: Ivanov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: T O
authors.initials: TO
authors.lastname: Pleshakova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Frantsuzov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Andreeva
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A L
authors.initials: AL
authors.lastname: Kaysheva
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Izotov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Pavlova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Ziborov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Radko
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000032:analysis / Q000737:chemistry*; D015699:HIV Envelope Protein gp120 / Q000032:analysis* / Q000276:immunology; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D055910:Antibodies, Immobilized; D052157:Aptamers, Nucleotide; D015699:HIV Envelope Protein gp120; D061925:Immobilized Nucleic Acids
keywords: aptamer; atomic force microscopy; fishing; gp120 HIV-1; mass spectrometry