title: Follicular hyperandrogenism downregulates aromatase in luteinized granulosa cells in polycystic ovary syndrome women.
abstract: Women with polycystic ovary syndrome (PCOS) undergoing IVF-embryo transfer based-assisted reproductive technology (ART) treatment show variable ovarian responses to exogenous FSH administration. For better understanding and control of PCOS ovarian responses in ART, the present study was carried out to compare the follicular hormones and the expression of granulosa cell genes between PCOS and non-PCOS women during ART treatment as well as their IVF outcomes. Overall, 138 PCOS and 78 non-PCOS women were recruited for the present study. Follicular fluid collected from PCOS women showed high levels of testosterone. The expression of aromatase was found significantly reduced in luteinized granulosa cells from PCOS women. In cultured luteinized granulosa cells isolated from non-PCOS women, their exposure to testosterone at a level that was observed in PCOS follicles could decrease both mRNA and protein levels of aromatase in vitro. The inhibitory effect of testosterone was abolished by androgen receptor antagonist, flutamide. These results suggest that the hyperandrogenic follicular environment may be a key hazardous factor leading to the down-regulation of aromatase in PCOS.
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Ye-Chun
authors.initials: YC
authors.lastname: Ruan
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Yun-jie
authors.initials: YJ
authors.lastname: Yang
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Liang
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Yi-bin
authors.initials: YB
authors.lastname: Han
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Teng
authors.affiliation: Shanghai First Maternity and Infant HospitalSchool of Medicine, Tongji University, Shanghai, ChinaEpithelial Cell Biology Research CentreSchool of Biomedical Sciences, Faculty of MedicineShenzhen Research InstituteThe Chinese University of Hong Kong, Hong Kong, China yangjianzhi@hotmail.com.
authors.forename: Jian-Zhi
authors.initials: JZ
authors.lastname: Yang
mesh_terms: D000328:Adult; D000726:Androgen Antagonists / Q000494:pharmacology; D001141:Aromatase / Q000378:metabolism*; D002460:Cell Line; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D005485:Flutamide / Q000494:pharmacology; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D017588:Hyperandrogenism / Q000378:metabolism*; D008184:Luteal Cells / Q000378:metabolism*; D014975:Lutein / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D027724:Reproductive Techniques, Assisted; D013739:Testosterone / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D000726:Androgen Antagonists; D012333:RNA, Messenger; D013739:Testosterone; D005485:Flutamide; D001141:Aromatase; C561410:CYP19A1 protein, human; D014975:Lutein