title: An experimental burn wound-healing study of non-thermal atmospheric pressure microplasma jet arrays.
abstract: In contrast with a thermal plasma surgical instrument based on coagulative and ablative properties, low-temperature (non-thermal) non-equilibrium plasmas are known for novel medicinal effects on exposed tissue while minimizing undesirable tissue damage. In this study we demonstrated that arrays of non-thermal microplasma jet devices fabricated from a transparent polymer can efficiently inactivate fungi (Candida albicans) as well as bacteria (Escherichia coli), both in vitro and in vivo, and that this leads to a significant wound-healing effect. Microplasma jet arrays offer several advantages over conventional single-jet devices, including superior packing density, inherent scalability for larger treatment areas, unprecedented material flexibility in a plasma jet device, and the selective generation of medically relevant reactive species at higher plasma densities. The therapeutic effects of our multi-jet device were verified on second-degree burns in animal rat models. Reduction of the wound area and the histology of the wound after treatment have been investigated, and expression of interleukin (IL)-1α, -6 and -10 was verified to evaluate the healing effects. The consistent effectiveness of non-thermal plasma treatment has been observed especially in decreasing wound size and promoting re-epithelialization through collagen arrangement and the regulation of expression of inflammatory genes.
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon, Republic of Korea.
authors.forename: Ok Joo
authors.initials: OJ
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon, Republic of Korea.
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Ju
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nanoscience and Technology and Polymer BIN Research Centre, Chonbuk National University, Republic of Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
authors.affiliation: Laboratory for Optical Physics and Engineering, Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL, USA.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Sun
authors.affiliation: Laboratory for Optical Physics and Engineering, Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Rivera
authors.affiliation: Laboratory for Optical Physics and Engineering, Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL, USA.
authors.forename: Jin Hoon
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Laboratory for Optical Physics and Engineering, Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL, USA.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory for Optical Physics and Engineering, Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL, USA.
authors.forename: J Gary
authors.initials: JG
authors.lastname: Eden
authors.affiliation: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon, Republic of Korea.|Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon, Gangwon, Republic of Korea.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D015415:Biomarkers / Q000378:metabolism; D002056:Burns / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology*; D002176:Candida albicans / Q000187:drug effects; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000187:drug effects; D007249:Inflammation / Q000235:genetics; D008297:Male; D058626:Plasma Gases / Q000494:pharmacology / Q000627:therapeutic use*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects / Q000235:genetics
chemical_list: D015415:Biomarkers; D058626:Plasma Gases; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1
keywords: biomedical; burn; device; microplasma; non-thermal; wound