title: Experimental evidences of the NO action on a recombinant PrxII F from pea plant and its effect preventing the citrate synthase aggregation.
abstract: S-nitrosylation is emerging as a key post-translational protein modification for the transduction of NO as a signaling molecule in plants. This data article supports the research article entitled "Functional and structural changes in plant mitochondrial PrxII F caused by NO" [1]. To identify the Cys residues of the recombinant PrxII F modified after the treatment with S-nitrosylating agents we performed the LC ESI-QTOF tandem MS and MALDI peptide mass fingerprinting analysis. Change in A 650 nm was monitored to estimate the thermal aggregation of citrate synthase in the presence S-nitrosylated PrxII F. The effect of the temperature on the oligomerization pattern and aggregation of PrxII F was analysed by SDS-PAGE and changes in absorbance at 650 nm, respectively.
authors.affiliation: CEBAS-CSIC, Department of Stress Biology and Plant Pathology, E-30100 Murcia, Spain.
authors.forename: Daymi
authors.initials: D
authors.lastname: Camejo
authors.affiliation: CEBAS-CSIC, Department of Stress Biology and Plant Pathology, E-30100 Murcia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Ortiz-Espín
authors.affiliation: EEZ-CSIC, Department of Biochemistry, Cellular and Molecular Biology of Plants, E-18080 Granada, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Lázaro
authors.affiliation: EEZ-CSIC, Department of Biochemistry, Cellular and Molecular Biology of Plants, E-18080 Granada, Spain.
authors.forename: María C
authors.initials: MC
authors.lastname: Romero-Puertas
authors.affiliation: EEZ-CSIC, Department of Biochemistry, Cellular and Molecular Biology of Plants, E-18080 Granada, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Lázaro-Payo
authors.affiliation: CEBAS-CSIC, Department of Stress Biology and Plant Pathology, E-30100 Murcia, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Sevilla
authors.affiliation: CEBAS-CSIC, Department of Stress Biology and Plant Pathology, E-30100 Murcia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Jiménez
keywords: Citrate synthase; Oligomerization; PrxII F; S-nitrosylation