title: Cost-efficient entrapment of β-glucosidase in nanoscale latex and silicone polymeric thin films for use as stable biocatalysts.
abstract: β-Glucosidase is an ubiquitous enzyme which has enormous biotechnological applications. Its deficiency in natural enzyme preparations is often overcome by exogenous supplementation, which further increases the enzyme utilization cost. Enzyme immobilization offers a potential solution through enzyme recycling and easy recovery. In the present work Aspergillus niger β-glucosidase is immobilized within nanoscale polymeric materials (polyurethane, latex and silicone), through entrapment, and subsequently coated onto a fibrous support. Highest apparent activity (90 U g(-1) polymer) was observed with latex, while highest entrapment efficiency (93%) was observed for the silicone matrix. Immobilization resulted in the thermo-stabilization of the β-glucosidase with an increase in optimum temperature and activation energy for cellobiose hydrolysis. Supplementation to cellulases also resulted in an increased cellulose hydrolysis, while retaining more than 70% functional stability. Hence, the current study describes novel preparations of immobilized β-glucosidase as highly stable and active catalysts for industrial food- and bio-processing applications.
authors.affiliation: Nanobiotechnology Lab, Department of Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USA; Department of Bioinformatics and Biotechnology, Government College University Faisalabad (GCUF), Allama Iqbal Road, 38000 Faisalabad, Pakistan. Electronic address: rizwan@gcuf.edu.pk.
authors.forename: Muhammad Rizwan
authors.initials: MR
authors.lastname: Javed
authors.affiliation: c-LEcta GmbH, Perlickstraße 5, 04103 Leipzig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Buthe
authors.affiliation: Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad, Pakistan.
authors.forename: Muhammad Hamid
authors.initials: MH
authors.lastname: Rashid
authors.affiliation: Nanobiotechnology Lab, Department of Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D003362:Cost-Benefit Analysis / Q000379:methods*; D007840:Latex / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012828:Silicones / Q000032:analysis / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D007840:Latex; D011108:Polymers; D012828:Silicones; D001617:beta-Glucosidase
keywords: Immobilization; Latex; Polyurethane; Silicone; β-Glucosidase