title: Outflow facility efficacy of five drugs in enucleated porcine eyes by a method of constant-pressure perfusion.
abstract: This study aimed to characterize a technique that assesses the outflow facility (C) efficacy of five kinds of IOP-lowering drugs commonly used clinically in enucleated porcine Eyes. Eyes were perfused at 15 mmHg with GPBS first to establish the baseline outflow facility (C0). Then the anterior chamber contents were exchanged for GPBS with corresponding concentration eye drops (4.9×10(3) nM Brimonidine, 41.1 nM Latanoprost, 3.4×10(3) nM Levobunolol, 3.0×10(3) nM Brinzolamide, 8.3×10(3) nM Pilocarpine) in five groups (n = 6 each), while 6 eyes received GPBS alone as control. The mean stable facility obtained after drug administration (C1) was continuously recorded. The changes between C0 and C1 (ΔC = C1-C0) were analyzed. Finally, for drugs among the five experiment groups with statistical significance, the concentration was reduced 3 times, otherwise the drugs' concentration was increased to 10 times to confirm its effectiveness further using the same methods (n = 6 each). We found that the average baseline outflow facility was 0.24±0.01 μl·min(-1)·mmHg(-1). C increased significantly in Brimonidine and Latanoprost groups, even the concentration of Brimonidine and Latanoprost was decreased 3 times (P < 0.05). However, there was no significantly increase in Levobunolol, Brinzolamide, Pilocarpine and control group (P > 0.05), but when drugs' concentration was increased to 10 times, the C value of Pilocarpine decreased significantly (P = 0.04). No significant washout effects in porcine eyes were observed. To conclude, outflow facility efficacy of five drugs in enucleated porcine eyes may provide a reference for clinical medicine. A constant-pressure perfusion technique should be useful to evaluate effect of pharmacologic agents or surgical manipulations on aqueous humor dynamics.
authors.affiliation: Department of Ophthalmology, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Shi
authors.affiliation: Department of Ophthalmology, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cong
authors.affiliation: Department of Ophthalmology, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Zhao-Zeng
authors.initials: ZZ
authors.lastname: Lu
authors.affiliation: Department of Ophthalmology, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Ophthalmology, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Yu-Yan
authors.initials: YY
authors.lastname: Zhang
keywords: Aqueous humor outflow facility; ocular hypotensive drugs; ocular perfusion; porcine eyes