title: Effects of dietary extra virgin olive oil and its fractions on antioxidant status and DNA damage in the heart of rats co-exposed to aluminum and acrylamide.
abstract: Oxidative stress generated by an excessive production of free radicals has been linked to the development of several health problems such as cardiovascular diseases. We investigated the protective efficacy of Extra Virgin Olive Oil (EVOO) and its lipophilic fraction (OOLF) and hydrophilic fraction (OOHF) against the cardiotoxicity and DNA damage induced by co-exposure to aluminum (AlCl3) and acrylamide (ACR). Rats were divided into eight groups of six each: controls, AlCl3 (50 mg per kg body weight) administered via drinking water and ACR (20 mg per kg body weight) given by gavage, combined group plus EVOO (300 μl); combined group plus the hydrophilic fraction (1 ml); combined group plus the lipophilic fraction (300 μl); extra virgin olive oil (EVOO) and its fractions were administered daily by gavage for 21 days. Three other groups, considered as positive controls, received either EVOO, OOLF or OOLH. Exposure of rats to both AlCl3 and ACR provoked oxidative stress objectified by an increase in MDA, AOPP and a decrease in GSH, NPSH and vitamin C levels. The activities of CAT, GPx and SOD were also decreased. EVOO and its OOLF fraction exhibited a pronounced enhancement of antioxidant status while a partial recovery in the antioxidant status was obtained with the OOHF fraction. Plasma LDH and CK activities, TC, LDL-C levels, TC/HDL-C and LDL-C/HDL-C ratios were increased, while HDL-C and TG decreased in rats treated with both AlCl3 and ACR. Co-administration of EVOO, OOLF or OOHF to treated rats restored cardiac biomarkers and lipid profile to near-normal values. Histological studies and DNA damage confirmed the biochemical parameters and the beneficial role of EVOO and its two fractions. Our results suggest that extra virgin olive oil and its two fractions can decrease the frequency of cardiac complications and genotoxicity.
authors.affiliation: Animal Physiology Laboratory, Sfax Faculty of Sciences, University of Sfax, 3000 Sfax, BP 1171, Tunisia. Naj_Zgh@yahoo.fr.
authors.forename: Imen
authors.initials: I
authors.lastname: Ghorbel
authors.forename: Mouna
authors.initials: M
authors.lastname: Khemakhem
authors.forename: Ons
authors.initials: O
authors.lastname: Boudawara
authors.forename: Rim
authors.initials: R
authors.lastname: Marrekchi
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.forename: Raja
authors.initials: R
authors.lastname: Ben Amar
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
authors.forename: Naziha
authors.initials: N
authors.lastname: Grati Kamoun
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002318:Cardiovascular Diseases / Q000139:chemically induced / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D006321:Heart / Q000187:drug effects; D006801:Humans; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D031658:Olea / Q000378:metabolism; D000069463:Olive Oil / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats
chemical_list: D000069463:Olive Oil; D020106:Acrylamide; D000535:Aluminum