title: A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production.
abstract: Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies.
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC1245 (7600) Mar del Plata, Argentina.
authors.forename: Vanesa E
authors.initials: VE
authors.lastname: Tossi
authors.affiliation: UMYMFOR - Departamento de Química Orgánica y CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Ciudad de Buenos Aires C1428EGA, Argentina.
authors.forename: Sofía L
authors.initials: SL
authors.lastname: Acebedo
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC1245 (7600) Mar del Plata, Argentina.
authors.forename: Raúl O
authors.initials: RO
authors.lastname: Cassia
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC1245 (7600) Mar del Plata, Argentina.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lamattina
authors.affiliation: UMYMFOR - Departamento de Química Orgánica y CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Ciudad de Buenos Aires C1428EGA, Argentina.
authors.forename: Lydia R
authors.initials: LR
authors.lastname: Galagovsky
authors.affiliation: UMYMFOR - Departamento de Química Orgánica y CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Ciudad de Buenos Aires C1428EGA, Argentina. Electronic address: jar@qo.fcen.uba.ar.
authors.forename: Javier A
authors.initials: JA
authors.lastname: Ramírez
mesh_terms: D001681:Biological Assay / Q000379:methods*; D060406:Brassinosteroids / Q000032:analysis*; D005470:Fluorometry / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism*; D013260:Steroids, Heterocyclic / Q000032:analysis*; D003313:Zea mays / Q000166:cytology / Q000378:metabolism*
chemical_list: D060406:Brassinosteroids; D013260:Steroids, Heterocyclic; D009569:Nitric Oxide; C023623:brassinolide
keywords: Bioactivity assay; Brassinosteroids; Nitric oxide