title: Weissella oryzae DC6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential.
abstract: Nanoparticles and nanomaterials are at the prominent edge of the rapidly developing field of nanotechnology. Recently, nanoparticle synthesis using biological resources has been found to be a new area with considerable prospects for development. Biological systems are the masters of ambient condition chemistry and are able to synthesize nanoparticles by utilizing metal salts. In the perspective of the current initiative to develop green technologies for the synthesis of nanoparticles, microorganisms are of considerable interest. Thus, the present study describes a bacterial strain-Weissella oryzae DC6-isolated from mountain ginseng, for the green and facile synthesis of silver nanoparticles. The particles were synthesized effectively without the need for any supplementary modification to maintain stability. The synthesized nanoparticles were evaluated by several instrumental techniques, comprising ultraviolet-visible spectrophotometry, field emission transmission electron microscopy, energy dispersive X-ray spectroscopy, elemental mapping, X-ray diffraction, and dynamic light scattering. In addition, the biosynthesized silver nanoparticles were explored for their antimicrobial activity against clinical pathogens including Vibrio parahaemolyticus, Bacillus cereus, Bacillus anthracis, Staphylococcus aureus, Escherichia coli, and Candida albicans. Furthermore, the potential of nanoparticles has been observed for biofilm inhibition against Staphylococcus aureus and Pseudomonas aeruginosa. Thus, the synthesis of silver nanoparticles by the strain W. oryzae DC6 may serve as a simple, green, cost-effective, consistent, and harmless method to produce antimicrobial silver nanoparticles.
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Yeon J
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: b Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University , Republic of Korea.
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.|b Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University , Republic of Korea.
authors.forename: Deok C
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000254:growth & development*; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D058836:Weissella / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D012834:Silver
keywords: Weissella oryzae DC6; antimicrobial effect; biofilm inhibition; silver nanoparticles