title: Development and functional characterization of alginate dressing as potential protein delivery system for wound healing.
abstract: This study aimed to develop and characterize stable films as potential protein delivery dressings to wounds. Films were prepared from aqueous gels of sodium alginate (SA) and glycerol (GLY) (SA:GLY 1:0, 1:1, 1:2, 2:3, 2:1, 4:3). Purified recombinant glutathione-s-transferase (GST), green fluorescent protein (GFP) and GST fused in frame to GFP (GST-GFP) (model proteins) were characterized (SDS PAGE, Western blotting, immune-detection, and high sensitivity differential scanning calorimetry) and loaded (3.3, 6.6 and 30.2mg/g of film) into SA:GLY 1:2 film. These were characterized using texture analysis, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy, swelling, adhesion, dissolution and circular dichroism (CD). The protein loaded dressings were uniform, with a good balance between flexibility and toughness. The films showed ideal moisture content required for protein conformation (TGA), interactions between proteins and film components (DSC), indicating stability which was confirmed by CD. Swelling and adhesion showed that formulations containing 6.6mg/g of protein possessed ideal characteristics and used for in vitro dissolution studies. Protein release was rapid initially and sustained over 72h and data fitted to various kinetic equations showed release followed zero-order and Fickian diffusion. The results demonstrate the potential of SA dressings for delivering therapeutic proteins to wounds.
authors.affiliation: Department of Pharmaceutical Chemical and Environmental Sciences, Faculty of Engineering and Science, University of Greenwich at Medway, Central Avenue, Chatham Maritime, ME4 4TB Kent, UK.
authors.forename: Frederick U
authors.initials: FU
authors.lastname: Momoh
authors.affiliation: Department of Pharmaceutical Chemical and Environmental Sciences, Faculty of Engineering and Science, University of Greenwich at Medway, Central Avenue, Chatham Maritime, ME4 4TB Kent, UK. Electronic address: J.S.Boateng@gre.ac.uk.
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Boateng
authors.affiliation: Department of Life and Sports Sciences, Faculty of Engineering and Science, University of Greenwich at Medway, Central Avenue, Chatham Maritime, ME4 4TB Kent, UK.
authors.forename: Simon C W
authors.initials: SC
authors.lastname: Richardson
authors.affiliation: Department of Life and Sports Sciences, Faculty of Engineering and Science, University of Greenwich at Medway, Central Avenue, Chatham Maritime, ME4 4TB Kent, UK.
authors.forename: Babur Z
authors.initials: BZ
authors.lastname: Chowdhry
authors.affiliation: Department of Pharmaceutical Chemical and Environmental Sciences, Faculty of Engineering and Science, University of Greenwich at Medway, Central Avenue, Chatham Maritime, ME4 4TB Kent, UK.
authors.forename: John C
authors.initials: JC
authors.lastname: Mitchell
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004355:Drug Stability; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry; D012995:Solubility; D013718:Tensile Strength; D013818:Thermogravimetry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011506:Proteins; D011994:Recombinant Proteins; D020723:Glucuronic Acid
keywords: Alginate dressing; GST-GFP proteins; Wound healing