title: Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification.
abstract: Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus . This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow's uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after Hind III digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes Hind III and Bam HI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of Bam HI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
authors.affiliation: Centro de Diagnóstico Marcos Enrietti, Curitiba, PR, Brazil. ; Programa de Pós Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Ernesto Renato
authors.initials: ER
authors.lastname: Kruger
authors.affiliation: Instituto de Tecnologia do Paraná, Curitiba, PR, Brazil. ; Programa de Pós Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Tania Regina
authors.initials: TR
authors.lastname: Penha
authors.affiliation: Centro de Microscopia Eletrônica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Daura Regina Eira
authors.initials: DR
authors.lastname: Stoffelo
authors.affiliation: Departamento de Microbiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
authors.affiliation: Departamento de Patologia Básica, Centro Politécnico, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Magda Costa
authors.initials: MC
authors.lastname: Ribeiro
authors.affiliation: Programa de Pós Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Vanete Thomaz
authors.initials: VT
authors.lastname: Soccol
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D005122:Exudates and Transudates / Q000821:virology; D005260:Female; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D026021:Herpesvirus 4, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D046529:Microscopy, Electron, Transmission; D012150:Polymorphism, Restriction Fragment Length; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology; D014599:Uterus / Q000473:pathology / Q000821:virology; D014771:Virion / Q000648:ultrastructure; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral
keywords: BoHV-4 virus; PCR; RFLP; fingerprint