title: Mapping and recombination analysis of two moth colour mutations, Black moth and Wild wing spot, in the silkworm Bombyx mori.
abstract: Many lepidopteran insects exhibit body colour variations, where the high phenotypic diversity observed in the wings and bodies of adults provides opportunities for studying adaptive morphological evolution. In the silkworm Bombyx mori, two genes responsible for moth colour mutation, Bm and Ws, have been mapped to 0.0 and 14.7 cM of the B. mori genetic linkage group 17; however, these genes have not been identified at the molecular level. We performed positional cloning of both genes to elucidate the molecular mechanisms that underlie the moth wing- and body-colour patterns in B. mori. We successfully narrowed down Bm and Ws to ~2-Mb-long and 100-kb-long regions on the same scaffold Bm_scaf33. Gene prediction analysis of this region identified 77 candidate genes in the Bm region, whereas there were no candidate genes in the Ws region. Fluorescence in-situ hybridisation analysis in Bm mutant detected chromosome inversion, which explains why there are no recombination in the corresponding region. The comparative genomic analysis demonstrated that the candidate regions of both genes shared synteny with a region associated with wing- and body-colour variations in other lepidopteran species including Biston betularia and Heliconius butterflies. These results suggest that the genes responsible for wing and body colour in B. mori may be associated with similar genes in other Lepidoptera.
authors.affiliation: Department of Science of Biological Production, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katsuma
authors.affiliation: Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kuwazaki
authors.affiliation: Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Jouraku
authors.affiliation: Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujimoto
authors.affiliation: Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sahara
authors.affiliation: Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yasukochi
authors.affiliation: Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Science of Biological Production, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tabunoki
authors.affiliation: Department of Science of Biological Production, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.affiliation: Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kadono-Okuda
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: T
authors.identifier: http://orcid.org/0000-0002-5791-0000
authors.initials: T
authors.lastname: Shimada
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D017344:Genes, Insect; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D009154:Mutation; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D011995:Recombination, Genetic; D026801:Synteny; D014921:Wings, Animal