title: Diversity of Saccharomyces cerevisiae strains isolated from Borassus akeassii palm wines from Burkina Faso in comparison to other African beverages.
abstract: In South-West of Burkina Faso, palm wine is produced by spontaneous fermentation of the sap from a specific palm tree Borassus akeassii and plays an important role in people's lives. Saccharomyces cerevisiae is the main agent of this alcoholic fermentation but little is known about the diversity of the isolates from palm. In this work, 39 Saccharomyces cerevisiae strains were isolated from palm wine samples collected from 14 sites in Burkina Faso, as well as 7 isolates obtained from sorghum beer (Dolo) from 3 distant sites. Their diversity was analyzed at 12 microsatellite loci, and compared to the genotypes obtained for other African yeast populations isolated from Cocoa hulks from Ghana, sorghum beer from Ivory Coast, palm wine from Djibouti Republic, and to our database of strains from miscellaneous origins (bread, beer, wine, sake, oaks…). The ploidy of these strains has been assessed as well by flow cytometry. Our results show that B. akeassii palm wine contains a specific yeast population of diploid strains, different from Dolo produced in the same area and from other palm wine strains from Ivory Coast, Nigeria, or Djibouti Republic. In contrast, Dolo strains appeared as a group of related and mainly tetraploid strains despite being isolated from different countries.
authors.affiliation: Laboratory of Microbiology and Biotechnology, Research Center in Biological, Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University of Ouagadougou, Burkina Faso; INRA, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France. Electronic address: tapsobaf@gmail.com.
authors.forename: François
authors.initials: F
authors.lastname: Tapsoba
authors.affiliation: INRA, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France; Montpellier SupAgro, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France; Montpellier University, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Legras
authors.affiliation: Laboratory of Food Technology, Department of Biochemistry and Microbiology, University of Ouagadougou, Burkina Faso.
authors.forename: Aly
authors.initials: A
authors.lastname: Savadogo
authors.affiliation: INRA, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France; Montpellier SupAgro, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France; Montpellier University, UMR1083 Sciences pour l'Œnologie, 2 Place Viala, F-34060 Montpellier, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dequin
authors.affiliation: Laboratory of Microbiology and Biotechnology, Research Center in Biological, Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University of Ouagadougou, Burkina Faso; Laboratory of Food Technology, Department of Biochemistry and Microbiology, University of Ouagadougou, Burkina Faso.
authors.forename: Alfred Sababenedyo
authors.initials: AS
authors.lastname: Traore
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis / Q000382:microbiology*; D028023:Arecaceae / Q000378:metabolism / Q000382:microbiology*; D002050:Burkina Faso; D005285:Fermentation; D014644:Genetic Variation; D005838:Genotype; D005869:Ghana; D018895:Microsatellite Repeats; D009549:Nigeria; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
keywords: Borassus akeassii; Diversity; Flow cytometry; Microsatellite typing; Palm wine; Saccharomyces cerevisiae