title: Effect of lignocellulose-derived inhibitors on the growth and D-lactic acid production of Sporolactobacillus inulinus YBS1-5.
abstract: The impact of lignocellulose-derived inhibitors on the cell growth and D-lactic production of Sporolactobacillus inulinus YBS1-5 was investigated. At high concentrations, both furans and phenolics, such as furfural, HMF, syringaldehyde and vanillin, affected cell growth and D-lactic acid production and syringaldehyde exhibited the highest. Further experiments showed that only vanillin caused cellular membrane damage. Based on the Biolog approach, in vivo studies on intact S. inulinus cells indicated that phenolics had a stronger inhibitory effect than furan derivatives on the metabolic activity of the concerned substrates related with the key enzymes of D-lactic acid fermentation. The direct in vitro inhibitory effect of the model compounds on the four key enzymes displayed similar patterns. Syringaldehyde was the strongest inhibitor. In general, comparison with published results for other microorganisms indicated that strain YBS1-5 was a robust microorganism against inhibitors of lignocellulose hydrolysate. Notably, in concentrated corn stover hydrolysate, S. inulinus YBS1-5 produced 70.7 g/L D-lactic acid, which was 87.7 % of the yield from the control experiment. However, the fermentation time was prolonged 36 h. In order to improve fermentation rate, a detoxification technology or more robust mutant to phenolics especially syringaldehyde should be developed.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan road, Nanjing, 211816, Jiangsu, China.
authors.forename: Zhongzhong
authors.initials: Z
authors.lastname: Bai
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan road, Nanjing, 211816, Jiangsu, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan road, Nanjing, 211816, Jiangsu, China.
authors.forename: Bingfang
authors.initials: B
authors.lastname: He
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan road, Nanjing, 211816, Jiangsu, China. wubin1977@njtech.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D056449:Bacillales / Q000145:classification / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000008:administration & dosage* / Q000737:chemistry; D019344:Lactic Acid / Q000096:biosynthesis*; D008031:Lignin / Q000737:chemistry; D010636:Phenols / Q000008:administration & dosage* / Q000737:chemistry; D013045:Species Specificity; D003313:Zea mays / Q000382:microbiology*
chemical_list: D005663:Furans; D010636:Phenols; C036909:lignocellulose; D019344:Lactic Acid; D008031:Lignin
keywords: Corn stover hydrolysate; D-Lactic acid; Fermentation inhibitors; Sporolactobacillus inulinus