title: ADCY7 supports development of acute myeloid leukemia.
abstract: Acute myeloid leukemia (AML) is the most common adult acute leukemia. Despite treatment, the majority of the AML patients relapse within 5 years. In silico analysis of several available databases of AML patients showed that the expression of adenylate cyclase 7 (ADCY7) significantly inversely correlates with the overall survival of AML patients. To determine whether ADCY7 supports AML development, we employed an shRNA-encoding lentivirus system to inhibit adcy7 expression in human AML cells including U937, MV4-11, and THP-1 cells. The ADCY7 deficiency resulted in decreased cell growth, elevated apoptosis, and lower c-Myc expression of these leukemia cells. This indicates that G protein-coupled receptor signaling contributes to AML pathogenesis. Our study suggests that inhibition of ADCY7 may be novel strategy for treating leukemia.
authors.affiliation: Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong 264003, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Li
authors.affiliation: Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong 264003, China; Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong 264003, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong 264003, China.
authors.forename: Yancun
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong 264003, China.
authors.forename: Renhui
authors.initials: R
authors.lastname: Zhan
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong 264003, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: Alec.Zhang@UTSouthwestern.edu.
authors.forename: Cheng Cheng
authors.initials: CC
authors.lastname: Zhang
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D015973:Gene Expression Regulation, Leukemic; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D016019:Survival Analysis
chemical_list: C118925:ADGRG1 protein, human; C088832:CCDC6 protein, human; D003598:Cytoskeletal Proteins; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; D043562:Receptors, G-Protein-Coupled; D000262:Adenylyl Cyclases; C473265:adenylyl cyclase 7
keywords: ADCY7; Acute myeloid leukemia; Apoptosis; Cell growth; G protein-coupled receptor; shRNA