title: Effect of Orexin-A infusion in to the Nucleus Accumbens on consummatory behaviour and alcohol preference in male Wistar rats.
abstract: BACKGROUND
  Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown.
  
  
  METHODS
  In this study, Male Wistar albino rats (n = 54) weighing about 250 ± 10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9%) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10%) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test.
  
  
  RESULTS
  Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p < 0.05) and alcohol (p < 0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol. CONCLUSIONS FOR: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.
authors.forename: Santosh
authors.initials: S
authors.lastname: Mayannavar
authors.forename: K S
authors.initials: KS
authors.lastname: Rashmi
authors.forename: Yalla Durga
authors.initials: YD
authors.lastname: Rao
authors.forename: Saraswati
authors.initials: S
authors.lastname: Yadav
authors.forename: B
authors.initials: B
authors.lastname: Ganaraja
mesh_terms: D000428:Alcohol Drinking / Q000188:drug therapy*; D000818:Animals; D001522:Behavior, Animal; D002755:Choice Behavior / Q000187:drug effects*; D003259:Consummatory Behavior / Q000187:drug effects*; D004435:Eating / Q000187:drug effects; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology; D000068797:Orexins; D051381:Rats; D017208:Rats, Wistar
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins