title: Estimation of Functional Activity of Ionized Calcium for Complement System Reactivity Assessment.
abstract: We proposed a new indicator for evaluation of functional activity of Ca(2+) in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37°C for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са(2+) activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca(2+) make possible estimation of the individual's immune status.
authors.affiliation: P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia, shoibonov@mail.ru.
authors.forename: B B
authors.initials: BB
authors.lastname: Shoibonov
authors.forename: D V
authors.initials: DV
authors.lastname: Grigor'eva
authors.forename: A A
authors.initials: AA
authors.lastname: Shabalina
authors.forename: M V
authors.initials: MV
authors.lastname: Kostyreva
authors.forename: V Yu
authors.initials: VY
authors.lastname: Baronets
authors.forename: T S
authors.initials: TS
authors.lastname: Zamolodchikova
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology*; D003167:Complement Activation / Q000187:drug effects; D004492:Edetic Acid / Q000737:chemistry; D004533:Egtazic Acid / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D012756:Sheep
chemical_list: D004533:Egtazic Acid; D004492:Edetic Acid; D002118:Calcium