title: Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions.
abstract: We report a centrifugal microfluidic method, droplet-shooting and size-filtration (DSSF), for the production of cell-sized liposomes with controlled lipid compositions. This involves the generation of large and small droplets from the tip of a glass capillary and the selective transfer of small droplets through an oil-water interface, thus resulting in the generation of cell-sized liposomes. We demonstrate control of the microdomain formation as well as the formation of asymmetric lipid bilayer liposomes of uniform size by the control of lipid composition. The DSSF method involves simple microfluidics and is easy to use. In addition, only a small volume (0.5-2 μL) of sample solution is required for the formation of hundreds of cell-sized liposomes. We believe that this method can be applied to generate cell-sized liposomes for a wide variety of uses, such as the construction of artificial cell-like systems.
authors.affiliation: Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology, Kanagawa, 226-8502, Japan.
authors.forename: Masamune
authors.initials: M
authors.lastname: Morita
authors.affiliation: Department of Mechanical Engineering, Keio University, Kanagawa, 223-8522, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onoe
authors.affiliation: Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo, 184-8588, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Yanagisawa
authors.affiliation: Department of Physics, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ito
authors.affiliation: Department of Physics, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ichikawa
authors.affiliation: Department of Bioscience and Informatics, Keio University, Kanagawa, 223-8522, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Fujiwara
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, 606-8507, Japan.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Saito
authors.affiliation: Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology, Kanagawa, 226-8502, Japan. takinoue.m.aa@m.titech.ac.jp.|PRESTO, Japan Science and Technology Agency, Saitama, 332-0012, Japan. takinoue.m.aa@m.titech.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takinoue
mesh_terms: D002498:Centrifugation / Q000295:instrumentation*; D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes
keywords: asymmetric lipid bilayers; centrifugal capillary-based microfluidic device; fluorescence spectroscopy; liposomes; membranes