title: Effect of mesoporous silica nanoparticles on cell viability and markers of oxidative stress.
abstract: In the recent years, the use of mesoporous silica nanoparticles (MSNs) has been extended in biomedical fields such as cancer therapy, drug and gene delivery, biosensors, and enzyme immobilization. Although nanomaterials are currently being widely used in modern technology, there is a lack of information regarding to the health and environmental implications of manufactured nanomaterials. In the present study, the effects of MSNs and surface functionalized MSNs on cell viability, markers of oxidative damages (mainly intracellular reactive oxygen species (ROS) formation), and oxidative DNA damage were investigated in vitro in rat pheochromocytoma PC12 cells. Following exposure of these nanoparticles (1.95-1000 µg/mL) to PC12 cells for 12 and 24 h, no significant reduction of cell viability was observed compared with control. Moreover, ROS formation and oxidative DNA damage were not significantly changed by these nanoparticles even at high concentrations or prolong exposures. In conclusion, the results showed that neither MSNs nor functionalized MSNs exhibited any remarkable in vitro toxic properties in PC12 cells even at high concentration.
authors.affiliation: a Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences , Mashhad , Iran .
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Sadeghnia
authors.affiliation: b School of Pharmacy, Mashhad University of Medical Sciences , Mashhad , Iran .
authors.forename: Negar
authors.initials: N
authors.lastname: Zoljalali
authors.affiliation: c Department of Pharmacology , Cellular and Molecular Research Center, Birjand University of Medical Sciences , Birjand , Iran , and.
authors.forename: Mohammad Yahya
authors.initials: MY
authors.lastname: Hanafi-Bojd
authors.affiliation: b School of Pharmacy, Mashhad University of Medical Sciences , Mashhad , Iran .
authors.forename: Sara
authors.initials: S
authors.lastname: Nikoofal-Sahlabadi
authors.affiliation: d Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Bizhan
authors.initials: B
authors.lastname: Malaekeh-Nikouei
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D053758:Nanoparticles; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D018570:Risk Assessment; D012822:Silicon Dioxide / Q000737:chemistry / Q000633:toxicity*; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D012822:Silicon Dioxide
keywords: Cytotoxicity; DNA damage; mesoporous silica nanoparticles; nanotoxicity; reactive oxygen species