title: A mass spectrometry method for the determination of the species of origin of gelatine in foods and pharmaceutical products.
abstract: Gelatine is a component of a wide range of foods. It is manufactured as a by-product of the meat industry from bone and hide, mainly from bovine and porcine sources. Accurate food labelling enables consumers to make informed decisions about the food they buy. Since labelling currently relies heavily on due diligence involving a paper trail, there could be benefits in developing a reliable test method for the consumer industries in terms of the species origin of gelatine. We present a method to determine the species origin of gelatines by peptide mass spectrometry methods. An evaluative comparison is also made with ELISA and PCR technologies. Commercial gelatines were found to contain undeclared species. Furthermore, undeclared bovine peptides were observed in commercial injection matrices. This analytical method could therefore support the food industry in terms of determining the species authenticity of gelatine in foods.
authors.affiliation: Fera, Sand Hutton, York YO41 1LZ, UK. Electronic address: helen.grundy@fera.co.uk.
authors.forename: H H
authors.initials: HH
authors.lastname: Grundy
authors.affiliation: Fera, Sand Hutton, York YO41 1LZ, UK.
authors.forename: P
authors.initials: P
authors.lastname: Reece
authors.affiliation: BioArCh, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: M
authors.initials: M
authors.lastname: Buckley
authors.affiliation: BioArCh, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Solazzo
authors.affiliation: Technology Facility, Biology, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Dowle
authors.affiliation: Technology Facility, Biology, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: D
authors.initials: D
authors.lastname: Ashford
authors.affiliation: Fera, Sand Hutton, York YO41 1LZ, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Charlton
authors.affiliation: BioArCh, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: M K
authors.initials: MK
authors.lastname: Wadsley
authors.affiliation: BioArCh, University of York, Wentworth Way, York YO10 5DD, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Collins
mesh_terms: D000818:Animals; D002417:Cattle; D005780:Gelatin / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013552:Swine
chemical_list: D004364:Pharmaceutical Preparations; D005780:Gelatin
keywords: Authenticity; Bovine; Gelatine; Porcine; Spectrometry