title: Genetic analysis and molecular mapping of QTLs for resistance to rice black-streaked dwarf disease in rice.
abstract: Rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is transmitted by small brown planthoppers (Laodelphax striatellus Fallén, SBPH) and causes severe yield loss in epidemic years in China and other East Asian countries. Breeding for resistance to RBSDV is a promising strategy to control the disease. We identified Tetep that showed resistance to RBSDV using a field test and artificial inoculation test. An evaluation of the resistance mechanism revealed that Tetep was resistant to RBSDV but not to SBPH. Genetic analysis showed that the resistance of Tetep to RBSDV was controlled by quantitative trait loci (QTLs). Three new QTLs for RBSDV resistance were identified in this study, i.e., qRBSDV-3, qRBSDV-10 and qRBSDV-11. The LOD scores of qRBSDV-3, qRBSDV-10 and qRBSDV-11 were 4.07, 2.24 and 2.21, accounting for 17.5%, 0.3% and 12.4% of the total phenotypic variation, respectively. All the resistance loci identified in this study were associated with virus resistance genes. The alleles for enhancing resistance on chromosomes 3 and 11 originated from Tetep, whereas the other allele on chromosome 10 originated from a susceptible parent. The identified new resistance QTLs in this study are useful resources for efficiently breeding resistant rice cultivars to RBSDV.
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.|Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.|Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Du
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
authors.forename: Lijiao
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
authors.forename: Cunyi
authors.initials: C
authors.lastname: Gao
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.|Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.|Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.|Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D017343:Genes, Plant; D008040:Genetic Linkage; D012275:Oryza / Q000235:genetics* / Q000276:immunology; D040641:Quantitative Trait Loci; D012087:Reoviridae / Q000472:pathogenicity*