title: Association between mutations in gyrA and parC genes of Acinetobacter baumannii clinical isolates and ciprofloxacin resistance.
abstract: OBJECTIVE
  We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections.
  
  
  METHODS
  Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyrA and parC genes.
  
  
  RESULTS
  The 44 and 4 isolates of A. baumannii exhibited full and intermediate-resistant to ciprofloxacin, respectively. Overall, the 42 isolates with double mutations of gyrA and parC genes showed a higher level of ciprofloxacin resistance than the 3 isolates with single mutations of gyrA or parC.
  
  
  CONCLUSIONS
  Simultaneous mutations in gyrA and parC genes are expected to play a major role in high-level fluoroquinolone resistance in A. baumannii; albeit a single mutation in DNA topoisomerase IV could occasionally be associated with intermediate-resistance to these antimicrobials.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Abdollah
authors.initials: A
authors.lastname: Ardebili
authors.affiliation: Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Abdolaziz Rastegar
authors.initials: AR
authors.lastname: Lari
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Beheshti
authors.affiliation: Department of Science and Environment, Faculty of Science, Technology and Communication, Luxembourg University and Faculty of Science and Environment University of Liege, Liege, Belgium.
authors.forename: Elnaz Rastegar
authors.initials: ER
authors.lastname: Lari
keywords: Acinetobacter baumannii; Burn; Ciprofloxacin resistance; gyrA; parC