title: Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus).
abstract: Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher (P < 0.05) during peak summer, and iNOS and eNOS expressions were also observed to be significantly higher (P < 0.05) during peak winter season as compared with moderate season. The iNOS, eNOS, cNOS, HSP70, and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs. To conclude, data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats.
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Vijay Pratap
authors.initials: VP
authors.lastname: Yadav
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Satyaveer Singh
authors.initials: SS
authors.lastname: Dangi
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Vikrant Singh
authors.initials: VS
authors.lastname: Chouhan
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Saroj K
authors.initials: SK
authors.lastname: Dangi
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Gyanendra
authors.initials: G
authors.lastname: Singh
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Vijay Prakash
authors.initials: VP
authors.lastname: Maurya
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Puneet
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India. msarkar24@gmail.com.
authors.forename: Mihir
authors.initials: M
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D005260:Female; D006041:Goats / Q000235:genetics* / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018882:Heat Stress Disorders / Q000235:genetics* / Q000378:metabolism / Q000662:veterinary*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012621:Seasons
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase
keywords: Goat; HSP; Heat stress; NOS; PBMC