title: Glucuronoyl esterases are active on the polymeric substrate methyl esterified glucuronoxylan.
abstract: Alkali extracted beechwood glucuronoxylan methyl ester prepared by esterification of 4-O-methyl-D-glucuronic acid side residues by methanol was found to serve as substrate of microbial glucuronoyl esterases from Ruminococcus flavefaciens, Schizophyllum commune and Trichoderma reesei. The enzymatic deesterification was monitored by (1)H NMR spectroscopy and evaluated on the basis of the decrease of the signal of the ester methyl group and increase of the signal of methanol. The results show for the first time the action of enzymes on polymeric substrate, which imitates more closely the natural substrate in plant cell walls than the low molecular mass artificial substrates used up to present.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, 84538 Bratislava, Slovakia. Electronic address: chempbsa@savba.sk.
authors.forename: Peter
authors.initials: P
authors.lastname: Biely
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, 84538 Bratislava, Slovakia.
authors.forename: Anna
authors.initials: A
authors.lastname: Malovíková
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, 84538 Bratislava, Slovakia.
authors.forename: Iveta
authors.initials: I
authors.lastname: Uhliariková
authors.affiliation: Youtel Biotech Inc., Bothel, WA, USA.
authors.forename: Xin-Liang
authors.initials: XL
authors.lastname: Li
authors.affiliation: Western Regional Research Center, USDA-ARS, Albany, 94710 CA, USA.
authors.forename: Dominic W S
authors.initials: DW
authors.lastname: Wong
mesh_terms: D004950:Esterases / Q000737:chemistry* / Q000378:metabolism*; D004951:Esterification; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012567:Schizophyllum / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014990:Xylans; C038910:glucuronoxylan; D004950:Esterases
keywords: 4-O-methyl-d-glucuronic acid; Glucuronoxylan; Glucuronoxylan methyl ester; Glucuronoyl esterase; NMR