title: Persistent human Borna disease virus infection modifies the acetylome of human oligodendroglia cells towards higher energy and transporter levels.
abstract: BACKGROUND
  Borna disease virus (BDV) is a neurotropic RNA virus persistently infecting mammalian hosts including humans. Lysine acetylation (Kac) is a key protein post-translational modification (PTM). The unexpectedly broad regulatory scope of Kac let us to profile the entire acetylome upon BDV infection.
  
  
  METHODS
  The acetylome was profiled through stable isotope labeling for cell culture (SILAC)-based quantitative proteomics. The quantifiable proteome was annotated using bioinformatics.
  
  
  RESULTS
  We identified and quantified 791 Kac sites in 473 Kac proteins in human BDV Hu-H1-infected and non-infected oligodendroglial (OL) cells. Bioinformatic analysis revealed that BDV infection alters the acetylation of metabolic proteins, membrane-associated proteins and transmembrane transporter activity, and affects the acetylation of several lysine acetyltransferases (KAT).
  
  
  CONCLUSIONS
  Upon BDV persistence the OL acetylome is manipulated towards higher energy and transporter levels necessary for shuttling BDV proteins to and from nuclear replication sites.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Liv
authors.initials: L
authors.lastname: Bode
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chengyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Faculty of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, University-Town Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Jingjie PTM BioLab (Hangzhou) Co. Ltd, Hangzhou 310018, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
authors.affiliation: Advanced Institute of Translational Medicine, Tongji University, Shanghai 200092, China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address: xiepeng@cqmu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D051046:Amino-Acid N-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D001891:Borna disease virus / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019295:Computational Biology; D004734:Energy Metabolism; D005333:Fetus; D054884:Host-Pathogen Interactions; D006801:Humans; D007553:Isotope Labeling; D008239:Lysine / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000378:metabolism* / Q000821:virology; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D020543:Proteome; D051046:Amino-Acid N-Acetyltransferase; D008239:Lysine
keywords: Acetylation; BDV; Borna disease virus; Kac; Oligodendroglia; Proteomic