title: Brevirhabdus pacifica gen. nov., sp. nov., isolated from deep-sea sediment in a hydrothermal vent field.
abstract: A Gram-stain-negative, motile, aerobic bacterial strain, designated 22DY15T, was isolated from a deep-sea sediment sample collected from a hydrothermal vent field located in the East Pacific Rise. The isolate was a short rod with a single flagellum and was positive for catalase and oxidase activities. Q-10 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphoglycolipid, one aminolipid and three unidentified phospholipids. The principal fatty acid (>70 %) was C18 : 1ω7c. The genomic DNA G+C content was 64.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22DY15T represents a distinct lineage within the family Rhodobacteraceae. The closest relatives were species of the genera Aliiroseovarius (93.3–96.0 % 16S rRNA gene sequence similarity), Sulfitobacter (94.0–96.0 %) and Loktanella (92.0–95.9 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain 22DY15T could be differentiated from its most closely related genera. Therefore, it is proposed that strain 22DY15T represents a novel species in a new genus of the family Rhodobacteraceae, for which the name Brevirhabdus pacifica gen. nov., sp. nov. is proposed. The type strain of the type species is 22DY15T ( = JCM 19489T = DSM 27767T = CGMCC 1.12416T = MCCC 1K00276T).
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Wu
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Wang
authors.affiliation: Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Aharon
authors.initials: A
authors.lastname: Oren
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2