title: The microRNA-1246 promotes metastasis in non-small cell lung cancer by targeting cytoplasmic polyadenylation element-binding protein 4.
abstract: BACKGROUND
  The microRNAs present a class of non-coding RNAs which are usually implicated in tumor biology. Recent report has unraveled that a novel member of microRNA family called miR-1246. However, the functional role and molecular mechanisms of miR-1246 in non-small cell lung cancer (NSCLC) is still elusive.
  
  
  METHODS
  Using RT-PCR, luciferase reporter, mRNA microarrays, invasion and migration assays, we investigated the potential role of miR-1246 in the pathogenesis of NSCLC.
  
  
  RESULTS
  In this study, we showed that miR-1246 markedly promoted NSCLC cell migration and invasion. Meanwhile, we found that cytoplasmic polyadenylation element binding protein 4 (CPEB4) might be involved and serve as a direct target of miR-1246 in NSCLC. CPEB4 knockdown substantially enhanced NSCLC migration and invasion resembling the effect of miR-1246 in NSCLC. CPEB4 is also frequently downregulated in NSCLC and decreased CPEB4 expression correlated with poor survival.
  
  
  CONCLUSIONS
  These results suggested that the miR-1246 may promote cell metastasis by targeting CPEB4. Meanwhile, the level of CPEB4 could be used as a potential marker in NSCLC patients. Our findings unraveled novel functions of miR-1246 in lung cancer cells and shed light on NSCLC prognosis.
authors.affiliation: TCM-Integrated Hospital, Southern Medical University, Cancer Center, NO.13 Shiliugang Road, Haizhu District, Guangzhou, Guangdong, 510315, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Chemotherapy, Zhongshan People's Hospital, Zhongshan, Guangdong, 528400, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Li
authors.affiliation: TCM-Integrated Hospital, Southern Medical University, Cancer Center, NO.13 Shiliugang Road, Haizhu District, Guangzhou, Guangdong, 510315, China. jackieluorongcheng@163.com.
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Luo
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C554939:CPEB4 protein, human; C577700:MIRN1246 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins