title: miR-27 and miR-125 Distinctly Regulate Muscle-Enriched Transcription Factors in Cardiac and Skeletal Myocytes.
abstract: MicroRNAs are noncoding RNAs of approximately 22-24 nucleotides which are capable of interacting with the 3' untranslated region of coding RNAs (mRNAs), leading to mRNA degradation and/or protein translation blockage. In recent years, differential microRNA expression in distinct cardiac development and disease contexts has been widely reported, yet the role of individual microRNAs in these settings remains largely unknown. We provide herein evidence of the role of miR-27 and miR-125 regulating distinct muscle-enriched transcription factors. Overexpression of miR-27 leads to impair expression of Mstn and Myocd in HL1 atrial cardiomyocytes but not in Sol8 skeletal muscle myoblasts, while overexpression of miR-125 resulted in selective upregulation of Mef2d in HL1 atrial cardiomyocytes and downregulation in Sol8 cells. Taken together our data demonstrate that a single microRNA, that is, miR-27 or miR-125, can selectively upregulate and downregulate discrete number of target mRNAs in a cell-type specific manner.
authors.affiliation: Cardiovascular Research Group, Department of Experimental Biology, University of Jaén, 23071 Jaen, Spain.
authors.forename: Estefania
authors.initials: E
authors.lastname: Lozano-Velasco
authors.affiliation: Cardiovascular Research Group, Department of Experimental Biology, University of Jaén, 23071 Jaen, Spain.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Galiano-Torres
authors.affiliation: Cardiovascular Research Group, Department of Experimental Biology, University of Jaén, 23071 Jaen, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Jodar-Garcia
authors.affiliation: Cardiovascular Research Group, Department of Experimental Biology, University of Jaén, 23071 Jaen, Spain.
authors.forename: Amelia E
authors.initials: AE
authors.lastname: Aranega
authors.affiliation: Cardiovascular Research Group, Department of Experimental Biology, University of Jaén, 23071 Jaen, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Franco
mesh_terms: D000818:Animals; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse; C540674:Mirn27 microRNA, mouse; D009124:Muscle Proteins; D014157:Transcription Factors