title: A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary.
abstract: The ovary is a dynamic organ that undergoes dramatic remodeling throughout the ovulatory cycle. Maturation of the ovarian follicle, release of the oocyte in the course of ovulation as well as formation and degradation of corpus luteum involve tightly controlled remodeling of the extracellular matrix and vasculature. Ovarian tumors, regardless of their tissue of origin, dynamically interact with the ovarian microenvironment. Their activity in the tissue encompasses recruitment of host stroma and immune cells, attachment of tumor cells to mesothelial layer, degradation of the extracellular matrix and tumor cell migration. High-resolution dynamic imaging of such processes is particularly challenging for internal organs. The implementation of a novel imaging window as reported here enabled longitudinal microscopy of ovarian physiology and orthotopic tumor invasion.
authors.affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100 Israel.
authors.forename: Filip
authors.initials: F
authors.lastname: Bochner
authors.affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100 Israel.
authors.forename: Liat
authors.initials: L
authors.lastname: Fellus-Alyagor
authors.affiliation: Department of Veterinary Resources, Weizmann Institute, Rehovot 76100 Israel.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Kalchenko
authors.affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100 Israel.
authors.forename: Shiri
authors.initials: S
authors.lastname: Shinar
authors.affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100 Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Neeman
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D003338:Corpus Luteum / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D003911:Dextrans / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D009361:Neoplasm Invasiveness; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D010060:Ovulation / Q000502:physiology; D059016:Tumor Microenvironment
chemical_list: D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D003094:Collagen; D016650:Fluorescein-5-isothiocyanate