title: Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.
abstract: Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.
authors.affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Yongyuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Medical Examination Center, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Hu
authors.affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Chu
authors.initials: C
authors.lastname: Tang
authors.affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Sun
authors.affiliation: Orthopaedic Department, The Sixth Affiliated People's Hospital, Medical School of Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Xianlong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008297:Male; D049329:Nanostructures / Q000737:chemistry*; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects*; D011817:Rabbits; D013010:Sonication; D013499:Surface Properties; D023822:Tissue Engineering; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D014025:Titanium
keywords: in vivo; osseointegeration; titanium; ultrasonic shot peening