title: Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum.
abstract: Clostridium sporogenes DSM 795 is the type strain of the species Clostridium sporogenes, first described by Metchnikoff in 1908. It is a Gram-positive, rod-shaped, anaerobic bacterium isolated from human faeces and belongs to the proteolytic branch of clostridia. C. sporogenes attracts special interest because of its potential use in a bacterial therapy for certain cancer types. Genome sequencing and annotation revealed several gene clusters coding for proteins involved in anaerobic degradation of amino acids, such as glycine and betaine via Stickland reaction. Genome comparison showed that C. sporogenes is closely related to C. botulinum. The genome of C. sporogenes DSM 795 consists of a circular chromosome of 4.1 Mb with an overall GC content of 27.81 mol% harboring 3,744 protein-coding genes, and 80 RNAs.
authors.affiliation: Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University Göttingen, Göttingen, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Poehlein
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Riegel
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: König
authors.affiliation: Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University Göttingen, Göttingen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Leimbach
authors.affiliation: Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University Göttingen, Göttingen, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Daniel
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Dürre
keywords: Anaerobic; Butanol; C. botulinum; C. sporogenes; Gram-positive; Stickland reaction