title: Influence of the nanoprecipitation conditions on the supramolecular structure of squalenoyled nanoparticles.
abstract: Hydrophobic organic compounds dissolved in a polar solvent can self-assemble into nanoparticles (NPs) upon nanoprecipitation into water. In the present study, we have investigated the structure of squalenacetyl-adenosine (SQAc-Ad) nanoparticles which were previously found to exhibit impressive neuroprotective activity. When obtained by nanoprecipitation of a SQAc-Ad ethanolic solution into water, two different supramolecular organizations of SQAc-Ad NPs were evidenced, depending on the water-to-ethanol volume ratio. It has been shown that a fraction of the solvent remained associated with the NPs, despite prolonged evaporation under reduced pressure after nanoprecipitation, and that this residual solvent dramatically affected their structure. This study points to the importance of being in the "Ouzo" region to minimize the amount and effect of residual solvent and to control the structure of NPs.
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Lepeltier
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France. Electronic address: claudie.bourgaux@u-psud.fr.
authors.forename: Claudie
authors.initials: C
authors.lastname: Bourgaux
authors.affiliation: Elettra Sincrotrone, 34149 Basovizza, Trieste, Italy; Graz University Technology, Institut of Inorganic Chemistry, A-8010 Graz, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Amenitsch
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Rosilio
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Sinda
authors.initials: S
authors.lastname: Lepetre-Mouelhi
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Fatima
authors.initials: F
authors.lastname: Zouhiri
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Desmaële
authors.affiliation: Institut Galien Paris-Sud - UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud XI, 92296 Châtenay-Malabry Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Couvreur
mesh_terms: D000241:Adenosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D011232:Chemical Precipitation; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D015195:Drug Design; D000431:Ethanol / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D013185:Squalene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D018696:Neuroprotective Agents; D012996:Solutions; D012997:Solvents; C000610526:squalenacetyl-adenosine; D014867:Water; D000431:Ethanol; D013185:Squalene; D000241:Adenosine
keywords: Adenosine; High resolution synchrotron small-angle X-ray scattering (SAXS); Langmuir film; Nanoprecipitation; Neuroprotective activity; Nucleolipid; Squalenoylation; Supramolecular structure