title: Bioinspired Nanoparticulate Medical Glues for Minimally Invasive Tissue Repair.
abstract: Delivery of tissue glues through small-bore needles or trocars is critical for sealing holes, affixing medical devices, or attaching tissues together during minimally invasive surgeries. Inspired by the granule-packaged glue delivery system of sandcastle worms, a nanoparticulate formulation of a viscous hydrophobic light-activated adhesive based on poly(glycerol sebacate)-acrylate is developed. Negatively charged alginate is used to stabilize the nanoparticulate surface to significantly reduce its viscosity and to maximize injectability through small-bore needles. The nanoparticulate glues can be concentrated to ≈30 w/v% dispersions in water that remain localized following injection. With the trigger of a positively charged polymer (e.g., protamine), the nanoparticulate glues can quickly assemble into a viscous glue that exhibits rheological, mechanical, and adhesive properties resembling the native poly(glycerol sebacate)-acrylate based glues. This platform should be useful to enable the delivery of viscous glues to augment or replace sutures and staples during minimally invasive procedures.
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Chenjie
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Monisha
authors.initials: M
authors.lastname: Sebastin
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Albert
authors.initials: A
authors.lastname: Lee
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Holwell
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Calvin
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: David
authors.initials: D
authors.lastname: Miranda Nieves
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Luye
authors.initials: L
authors.lastname: Mu
authors.affiliation: Harvard-Massachusetts Institute of Technology, Division of HealthSciences and Technology, Institute for Medical Engineering & Science, Massachusetts Institute of Technology, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Langer
authors.affiliation: Wellman center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02140, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT Division of Health Science and Technology, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Karp
mesh_terms: D000818:Animals; D032701:Biomimetics / Q000379:methods*; D002417:Cattle; D057927:Hydrophobic and Hydrophilic Interactions; D007267:Injections; D008027:Light; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D014014:Tissue Adhesives / Q000494:pharmacology*; D014783:Viscosity; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D014014:Tissue Adhesives
keywords: injectability; medical adhesives; nanoparticles; sandcastle worm glue