title: Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI.
abstract: This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization methods are reviewed. The underlying mechanisms of the ionization techniques-namely, laser ablation of biological samples and electrospray ionization-as well as variations of the LAESI ion source for specific targets of interest are described.
authors.affiliation: Research Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology Jena, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bartels
authors.affiliation: Research Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology Jena, Germany.
authors.forename: Aleš
authors.initials: A
authors.lastname: Svatoš
keywords: ambient; electrospray; ionization; laser ablation; mass spectrometry