title: Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum.
abstract: Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90- 3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato.
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
authors.forename: W S
authors.initials: WS
authors.lastname: Zai
authors.affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: L X
authors.initials: LX
authors.lastname: Miao
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Xiong
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Ma
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Li
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Chen
authors.affiliation: Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China zaiwenshan@sina.com.
authors.forename: S G
authors.initials: SG
authors.lastname: Ye
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development; D005810:Multigene Family; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins