title: A diagnostic approach for identifying anti-neuronal antibodies in children with suspected autoimmune encephalitis.
abstract: We assessed the validity of immunoblotting, immunohistochemistry (IHC), and immunocytochemistry (ICC) to detect anti-neuronal antibodies in an attempt to establish a diagnostic approach for pediatric autoimmune encephalitis. Both IHC and ICC had higher sensitivity than immunoblotting and could differentiate between antibodies directed towards intracellular and cell surface antigens. There was a significant correlation between the IHC and ICC results. When patients were divided into encephalitis and non-encephalitis groups, there was no difference in the positivity rate and staining pattern of IHC and ICC between them. In conclusion, IHC and ICC are useful methods to screen for anti-neuronal antibodies. A combination of IHC, ICC, and specific cell-based assays is expected to be an efficient approach for the diagnosis of autoantibody-mediated encephalitis.
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, Japan; Department of Pediatrics, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Nakahara
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, Japan. Electronic address: sakuma-hs@igakuken.or.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakuma
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Kimura-Kuroda
authors.affiliation: Department of Pediatrics, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Department of Pediatrics, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan; Department of Pediatrics, Aichi Medical University Faculty of Medicine, 1-1 yazakokarimata, Nagakute, Aichi, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Okumura
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D001927:Brain Diseases / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004660:Encephalitis; D005260:Female; D050031:Hashimoto Disease / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D009474:Neurons / Q000276:immunology / Q000378:metabolism*; D017207:Rats, Sprague-Dawley
chemical_list: D001323:Autoantibodies; D015415:Biomarkers
keywords: Anti-neuronal antibody; Autoimmune encephalitis; Cell-based assay; Immunoblot; Immunocytochemistry; Immunohistochemistry