title: [Effect of oxygen-vectors on the production of ε-poly-L-lysine].
abstract: To enhance the production of ε-poly-L-lysine (ε-PL) by improving dissolved oxygen level of the fermentation system, different oxygen-vectors were added to broth and n-dodecane was screened as the best oxygen-vector. The best amount of n-dodecane was 0.5% (V/V) and the best time was at start of the fermentation. In a fed-batch fermentation in a 5 L bioreactor, ε-PL concentration reached a maximum of (30.8 ± 0.46) g/L and the dry cell weight obtained was (33.8 ± 0.29) g/L, increasing by 31.6% and 20.7% compared with the control group, respectively. This improvement can be related to 0.5% n-dodecane could maintain dissolved oxygen concentration > 32% of air concentration compared with 23.8% in ε-PL production phase, and the production of a main by-product, poly-L-diaminopropionic acid, fell by 31%. These results indicated that the dissolved oxygen level in the broth was improved by adding n-dodecane, which can inhibit the by-product production and improve the biosynthesis of ε-PL.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Bo
authors.forename: Zhaoxian
authors.initials: Z
authors.lastname: Xu
authors.forename: Zhuzhen
authors.initials: Z
authors.lastname: Sun
authors.forename: Changhong
authors.initials: C
authors.lastname: Cao
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Hong
authors.initials: H
authors.lastname: Xui
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Feng
mesh_terms: D000473:Alkanes / Q000737:chemistry; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D005285:Fermentation; D010100:Oxygen / Q000737:chemistry*; D011107:Polylysine / Q000096:biosynthesis*
chemical_list: D000473:Alkanes; C007548:n-dodecane; D011107:Polylysine; D010100:Oxygen