title: Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical-Sized Rat Calvaria Defect.
abstract: The gelatin (Gel) powders, derived from acidic and basic extractions of porcine dermis (referred to as AE and BE), were processed for the porous sponge preparation. The disks, which were less than or greater than 500 μm in diameter [small (S) and large (L) pores, respectively] in both extractions and had an interconnected structure respectively, were implanted in critical-sized defects (CSD) of rat calvaria for 4 and 8 weeks to analyze the bone repair capability. Only the AE-S disk induced bone formation (over 60%) histomorphometrically in the CSD after 8 weeks, although the collagen orientation of the regenerated bone was still immature. Osteoblastic cell culture until 14 days did not substantiate marked superiority of AE-S disk regarding the proliferation and the differentiation, although the initial attachment was enhanced on AE-S disk than BE-L disk. The results provide the findings that a Gel sponge with a specific porous structure is capable of inducing orthotopic bone formation in vivo environment.
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.|Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Kanda
authors.affiliation: Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Anada
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.|Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Takuto
authors.initials: T
authors.lastname: Handa
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.|Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.|Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Ezoe
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan. suzuki-o@m.tohoku.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D013552:Swine
chemical_list: D018786:Bone Substitutes; D005780:Gelatin
keywords: bone regeneration; gelatin; porous scaffold