title: Detection of G-type density in promoter sequence of colon cancer oncogenes and tumor suppressor genes.
abstract: The guanine rich locations are present in human genome. Previous studies have shown that the presence of G rich sequences and motifs may be significant for gene activity and function. We decided to focus our interest to identify G rich motifs in promoters of oncogenes and tumor suppressor genes. We used a set of 100 most common oncogenes and tumor suppressor genes (TSG) for this analysis. We collected 600nt long promoters with -500 and +100 TSS (transcription start site) from the oncogenes and TSG set. Using a computer program, we calculated the G densities using numbers and locations of G forms with 100nt moving widow. We included G numbers from 2 to 7 guanines. Analysis shows that G density increases from -500 to +100 and more from TSS. G density is found to be maximum within -/+100 of TSS. The results of G densities were compared with the expression data of the selected oncogenes and tumor suppressor genes in patients with colon cancer (n=174).
authors.affiliation: Department of Genetics and Bioengineering, International Burch University, Francuske revolucije BB, Ilidža 71000.
authors.forename: Senol
authors.initials: S
authors.lastname: Dogan
authors.affiliation: Department of Genetics and Bioengineering, International Burch University, Francuske revolucije BB, Ilidža 71000.
authors.forename: Anis
authors.initials: A
authors.lastname: Cilic
authors.affiliation: Department of Genetics and Bioengineering, International Burch University, Francuske revolucije BB, Ilidža 71000 ; Department of Clinical Pathology, Clinical Center of the University of Sarajevo, Bosnia and Herzegovina.
authors.forename: Amina
authors.initials: A
authors.lastname: Kurtovic-Kozaric
authors.affiliation: Department of Information Technologies, International Burch University, Francuske revolucije BB, Ilidža 71000, Sarajevo, Bosnia and Herzegovina.
authors.forename: Fatih
authors.initials: F
authors.lastname: Ozturk