title: Data set for the proteomic inventory and quantitative analysis of chicken uterine fluid during eggshell biomineralization.
abstract: Chicken eggshell is the protective barrier of the egg. It is a biomineral composed of 95% calcium carbonate on calcitic form and 3.5% organic matrix proteins. Mineralization process occurs in uterus into the uterine fluid. This acellular fluid contains ions and organic matrix proteins precursors which are interacting with the mineral phase and control crystal growth, eggshell structure and mechanical properties. We performed a proteomic approach and identified 308 uterine fluid proteins. Gene Ontology terms enrichments were determined to investigate their potential functions. Mass spectrometry analyses were also combined to label free quantitative analysis to determine the relative abundance of 96 proteins at initiation, rapid growth phase and termination of shell calcification. Sixty four showed differential abundance according to the mineralization stage. Their potential functions have been annotated. The complete proteomic, bioinformatic and functional analyses are reported in Marie et al., J. Proteomics (2015) [1].
authors.affiliation: INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l'œuf, F-37380 Nouzilly, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Marie
authors.affiliation: INRA, UMR INRA85, UMR CNRS 7247, Université de Tours, IFCE, Physiologie de la Reproduction et des Comportements, Plate-forme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Labas
authors.affiliation: INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l'œuf, F-37380 Nouzilly, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Brionne
authors.affiliation: INRA, UMR INRA85, UMR CNRS 7247, Université de Tours, IFCE, Physiologie de la Reproduction et des Comportements, Plate-forme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Harichaux
authors.affiliation: INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l'œuf, F-37380 Nouzilly, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Hennequet-Antier
authors.affiliation: INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l'œuf, F-37380 Nouzilly, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Nys
authors.affiliation: INRA, UR83 Recherches avicoles, Fonction et Régulation des protéines de l'œuf, F-37380 Nouzilly, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Gautron