title: SuRankCo: supervised ranking of contigs in de novo assemblies.
abstract: BACKGROUND
  Evaluating the quality and reliability of a de novo assembly and of single contigs in particular is challenging since commonly a ground truth is not readily available and numerous factors may influence results. Currently available procedures provide assembly scores but lack a comparative quality ranking of contigs within an assembly.
  
  
  RESULTS
  We present SuRankCo, which relies on a machine learning approach to predict quality scores for contigs and to enable the ranking of contigs within an assembly. The result is a sorted contig set which allows selective contig usage in downstream analysis. Benchmarking on datasets with known ground truth shows promising sensitivity and specificity and favorable comparison to existing methodology.
  
  
  CONCLUSIONS
  SuRankCo analyzes the reliability of de novo assemblies on the contig level and thereby allows quality control and ranking prior to further downstream and validation experiments.
authors.affiliation: Central Administration 4 (IT), Robert Koch Institute, Berlin, Germany. KuhringK@rki.de.|Centre for Biological Threats and Special Pathogens (ZBS 1), Robert Koch Institute, Berlin, Germany. KuhringK@rki.de.
authors.forename: Mathias
authors.initials: M
authors.lastname: Kuhring
authors.affiliation: Research Group Bioinformatics (NG4), Robert Koch Institute, Berlin, Germany. DabrowskiW@rki.de.|CAPES Foundation, Ministry of Education of Brazil, Brasília - DF, 70040-020, Brazil. DabrowskiW@rki.de.
authors.forename: Piotr Wojtek
authors.initials: PW
authors.lastname: Dabrowski
authors.affiliation: Centre for Biological Threats and Special Pathogens (ZBS 1), Robert Koch Institute, Berlin, Germany. PiroV@rki.de.
authors.forename: Vitor C
authors.initials: VC
authors.lastname: Piro
authors.affiliation: Research Group Bioinformatics (NG4), Robert Koch Institute, Berlin, Germany. NitscheA@rki.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Nitsche
authors.affiliation: Research Group Bioinformatics (NG4), Robert Koch Institute, Berlin, Germany. RenardB@rki.de.
authors.forename: Bernhard Y
authors.initials: BY
authors.lastname: Renard
mesh_terms: D000465:Algorithms; D020451:Contig Mapping / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D012372:ROC Curve; D012984:Software