title: Neuroprotection of resveratrol against neurotoxicity induced by methamphetamine in mouse mesencephalic dopaminergic neurons.
abstract: Resveratrol is originally extracted from huzhang, a Chinese herbal medicine. Recently, resveratrol has attracted a great of attention due to its antioxidant and antiapoptotic properties. Although the neuroprotection of resveratrol on neural damages in various models has been well characterized, little is known about the role of resveratrol in methamphetamine (MA) induced neurotoxicity in mesencephalic dopaminergic neurons. Dopaminergic neurons were isolated from midbrain of mouse embryos at embryonic day 15 and cultured in the presence of MA and resveratrol. Cell viability was examined by MTT assay and the apoptosis was assessed using Hoechst33342/PI double staining. To evaluate the Oxidative damage, ROS assay was performed. Moreover, the changes of time course of intracellular free calcium concentration ([Ca(2+) ]i) were analyzed with Fluo-3/AM tracing. The data showed that MA induced the neurotoxicity of cultured cells in a dose-dependent manner. Resveratrol significantly increased cellular viability and retarded cell apoptosis. Furthermore, resveratrol also attenuated MA induced ROS production and intracellular free calcium overload. Our results suggest that resveratrol protects dopaminergic neurons from MA-induced neuronal cytotoxicity, which, at least partly, is mediated by inhibition of [Ca(2+) ]i and oxidative stress. © 2015 BioFactors 41(4):252-260, 2015.
authors.affiliation: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Anatomy, Shandong University, School of Medicine, Jinan, Shandong, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Anatomy, Shandong University, School of Medicine, Jinan, Shandong, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Yue
authors.affiliation: Department of Radiology, The second Hoppital of Shandong University, Jinan, Shandong, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, the fourth hospital of Jinan City, Jinan, Shandong, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Anatomy, Shandong University, School of Medicine, Jinan, Shandong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Anatomy, Shandong University, School of Medicine, Jinan, Shandong, China.
authors.forename: Guibao
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Psychiatry School of Medicine, Shandong University, Jinan, Shandong, China.
authors.forename: Zengxun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Anatomy, Shandong University, School of Medicine, Jinan, Shandong, China.
authors.forename: Jinhao
authors.initials: J
authors.lastname: Sun
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism; D018765:Dopamine Uptake Inhibitors / Q000037:antagonists & inhibitors* / Q000633:toxicity; D059290:Dopaminergic Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004622:Embryo, Mammalian; D005456:Fluorescent Dyes; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008694:Methamphetamine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D061251:Primary Cell Culture; D017382:Reactive Oxygen Species / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D000975:Antioxidants; D018765:Dopamine Uptake Inhibitors; D005456:Fluorescent Dyes; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D013267:Stilbenes; D014966:Xanthenes; C059715:Fluo-3; D008694:Methamphetamine; D000077185:Resveratrol; D002118:Calcium; D004298:Dopamine
keywords: calcium; methamphetamine; oxidative stress; resveratrol