title: The 5-hydroxytryptamine transporter is functional in human coronary artery smooth muscle cells proliferation and is regulated by Interleukin-1 beta.
abstract: Abnormal human coronary artery smooth muscle cells (hCASMCs) proliferation and migration are key factors in coronary artery restenosis after percutaneous coronary intervention. Platelets release 5-hydroxytryptamine (5-HT), which is a strong mitogen for pulmonary artery smooth muscle cells proliferation and migration. Here, we investigated the effects of 5-HT and role of 5-HT transporter (5-HTT) on hCASMCs proliferation and migration. The 5-HT (10(-6)-10(-5) mol/l) significantly increased hCASMCs proliferation and migration, and these effects were inhibited by fluoxetine (10(-5) mol/l) and citalopram (10(-6) mol/l), two 5-HTT blocker. Overexpression in hCASMCs enhanced 5-HT induced cells proliferation and migration. The 5-HTT and interleukin-1 beta (IL-1β) expression were increased in rat balloon injury carotid arteries. Treatment with IL-1β (10 ng/ml, 3d) upregulates 5-HTT expression in hCASMCs and increased 5-HT induced currents in Human Embryonic Kidney 293-5-HTT cells.
authors.affiliation: Department of Cardiology, Zhongda Hospital Southeast University, Medical School of Southeast University Nanjing 210009, Jiangsu, China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Zhongda Hospital Southeast University, Medical School of Southeast University Nanjing 210009, Jiangsu, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Zhongda Hospital Southeast University, Medical School of Southeast University Nanjing 210009, Jiangsu, China.
authors.forename: Cheng-Chun
authors.initials: CC
authors.lastname: Tang
keywords: 5-hydroxytryptamine transporter; Human coronary artery smooth muscle cells; interleukin-1 beta