title: Thousand-fold volumetric concentration of live cells with a recirculating acoustofluidic device.
abstract: The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a "sample preparation" module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes.
authors.affiliation: †Department of Biomedical Engineering, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Ola
authors.initials: O
authors.lastname: Jakobsson
authors.affiliation: ‡Materials Department, Department of Chemical Engineering, Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, United States.
authors.forename: Seung Soo
authors.initials: SS
authors.lastname: Oh
authors.affiliation: †Department of Biomedical Engineering, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Antfolk
authors.affiliation: ‡Materials Department, Department of Chemical Engineering, Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Eisenstein
authors.affiliation: †Department of Biomedical Engineering, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
authors.affiliation: ‡Materials Department, Department of Chemical Engineering, Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, United States.
authors.forename: H Tom
authors.initials: HT
authors.lastname: Soh
mesh_terms: D000162:Acoustics; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*