title: Isolation and purification of rabbit mesenchymal stem cells using an optimized protocol.
abstract: Mesenchymal stem cells were first isolated and grown in vitro by Friedenstein over 40 yr ago; however, their isolation remains challenging as they lack unique markers for identification and are present in very small quantities in mesenchymal tissues and bone marrow. Using whole marrow samples, common methods for mesenchymal stem cell isolation are the adhesion method and density gradient fractionation. The whole marrow sample adhesion method still results in the nonspecific isolation of mononuclear cells, and activation and/or potential loss of target cells. Density gradient fractionation methods are complicated, and may result in contamination with toxic substances that affect cell viability. In the present study, we developed an optimized protocol for the isolation and purification of mesenchymal stem cells based on the principles of hypotonic lysis and natural sedimentation.
authors.affiliation: Guangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Lin
authors.affiliation: Guangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
authors.forename: Maorong
authors.initials: M
authors.lastname: Shen
authors.affiliation: Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Chen
authors.affiliation: Guangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Guangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
authors.forename: Daoming
authors.initials: D
authors.lastname: Luo
authors.affiliation: Guangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Cai
authors.affiliation: Guangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China. yangy062@sina.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D003470:Culture Media / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology*; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits
chemical_list: D003470:Culture Media
keywords: Hypotonic lysis; Isolation; Mesenchymal stem cells; Natural sedimentation; Purification