title: Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma.
abstract: Deregulation of microRNAs (miRNAs) is essential to tumor development, and serum miRNA profiles have been reported in several cancers. However, the serum miRNA profile in oral squamous cell carcinoma (OSCC) remained unclear. The present study aimed to explore abnormal miRNA profile in sera samples from OSCC patients and the association of miR-483-5p with patient prognosis. Microarray analysis was performed in sera from OSCC patients versus healthy controls. miR-483-5p expression was measured by reverse transcription (RT)-PCR and correlated to clinicopathological characteristics of OSCC patients. The prognostic significance was then evaluated with a Kaplan-Meier curve and log-rank tests, using a Cox proportional hazard model. According to microRNA array, 16 miRNAs were upregulated and 10 were downregulated in OSCC patient sera. miR-483-5p expression was significantly increased in OSCC patients (3.23-fold, p < 0.01), and this was significantly correlated with tumor nodal metastasis (TNM) stage and lymph nodal metastases (p < 0.01, p < 0.01). For predicting OSCC, receiver operating characteristic (ROC)/area under the curve (AUC) analysis confirmed a AUC of 0.85 (sensitivity of 0.853 and specificity of 0.746). OSCC patients with high serum miR-483-5p had lower survival than those with low expression, and multivariate analyses for overall survival revealed that high serum miR-483-5p expression was an independent prognostic factor for OSCC (HR = 2.32, 95 %CI 1.20-4.48). miR-483-5p expression increased in OSCC patient sera, and this may be a novel diagnostic and prognostic biomarker for OSCC.
authors.affiliation: Department of Stomatology, Shihua General Hospital of Lanzhou, 733 West Fuli Road, Gansu, China.
authors.forename: Huanxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Endodontics, Lanzhou Stomatology Hospital, Gansu, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Orthodontics, Lanzhou Stomatology Hospital, Gansu, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Anatomy and Embryology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Pathophysiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Yinxiang
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Institute of Pathophysiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Shihua General Hospital of Lanzhou, 733 West Fuli Road, Gansu, China. liningoral@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C548912:MIRN483 microRNA, human; D035683:MicroRNAs
keywords: Oral squamous cell carcinoma; Overall survival; Prognosis; miR-483-5p; microRNA