title: FRET sensor-based quantification of intracellular trehalose in mammalian cells.
abstract: Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIP-suc90μ∆1Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose.
authors.affiliation: a Insect Mimetics Research Unit , National Institute of Agrobiological Sciences , Tsukuba , Japan.|b Graduate School of Bio-Applications & Systems Engineering (BASE) , Tokyo University of Agriculture and Technology , Tokyo , Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kikuta
authors.affiliation: c Department of Plant Biology , Carnegie Institution for Science , Stanford , CA , USA.
authors.forename: Bi-Huei
authors.initials: BH
authors.lastname: Hou
authors.affiliation: b Graduate School of Bio-Applications & Systems Engineering (BASE) , Tokyo University of Agriculture and Technology , Tokyo , Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
authors.affiliation: c Department of Plant Biology , Carnegie Institution for Science , Stanford , CA , USA.
authors.forename: Wolf B
authors.initials: WB
authors.lastname: Frommer
authors.affiliation: a Insect Mimetics Research Unit , National Institute of Agrobiological Sciences , Tsukuba , Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kikawada
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004175:Diptera; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014199:Trehalose / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D019476:Insect Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D014199:Trehalose
keywords: FRET; biosensor; transporter; trehalose; visual quantification