title: Microarray-based identification of differentially expressed genes in extramammary Paget's disease.
abstract: Extramammary Paget's disease (EMPD) is a rare cutaneous malignancy accounting for approximately 1-2% of vulvar cancers. The rarity of this disease has caused difficulties in characterization and the molecular mechanism underlying EMPD development remains largely unclear. Here we used microarray analysis to identify differentially expressed genes in EMPD of the scrotum comparing with normal epithelium from healthy donors. Agilent single-channel microarray was used to compare the gene expression between 6 EMPD specimens and 6 normal scrotum epithelium samples. A total of 799 up-regulated genes and 723 down-regulated genes were identified in EMPD tissues. Real-time PCR was conducted to verify the differential expression of some representative genes, including ERBB4, TCF3, PAPSS2, PIK3R3, PRLR, SULT1A1, TCF7L1, and CREB3L4. Generally, the real-time PCR results were consistent with microarray data, and the expression of ERBB4, PRLR, TCF3, PIK3R3, SULT1A1, and TCF7L1 was significantly overexpressed in EMPD (P<0.05). Moreover, the overexpression of PRLR in EMPD, a receptor for the anterior pituitary hormone prolactin (PRL), was confirmed by immunohistochemistry. These data demonstrate that the differentially expressed genes from the microarray-based identification are tightly associated with EMPD occurrence.
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Jin-Ran
authors.initials: JR
authors.lastname: Lin
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Qiao-An
authors.initials: QA
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Shang-Shang
authors.initials: SS
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Qin
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Huashan Hospital Fudan University Shanghai 200040, China.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Xu
keywords: Extramammary Paget’s disease; differentially expressed genes; microarray-based identification