title: Review of matrix-assisted laser desorption ionization-imaging mass spectrometry for lipid biochemical histopathology.
abstract: Matrix-Assisted Laser Desorption Ionization-Imaging Mass Spectrometry (MALDI-IMS) is a rapidly evolving method used for the in situ visualization and localization of molecules such as drugs, lipids, peptides, and proteins in tissue sections. Therefore, molecules such as lipids, for which antibodies and other convenient detection reagents do not exist, can be detected, quantified, and correlated with histopathology and disease mechanisms. Furthermore, MALDI-IMS has the potential to enhance our understanding of disease pathogenesis through the use of "biochemical histopathology". Herein, we review the underlying concepts, basic methods, and practical applications of MALDI-IMS, including post-processing steps such as data analysis and identification of molecules. The potential utility of MALDI-IMS as a companion diagnostic aid for lipid-related pathological states is discussed.
authors.affiliation: Liver Research Center, Division of Gastroenterology and Department of Medicine (EBY, SMD)
authors.forename: Emine B
authors.initials: EB
authors.lastname: Yalcin
authors.affiliation: Liver Research Center, Division of Gastroenterology and Department of Medicine (EBY, SMD)|Departments of Neurology, Neurosurgery, and Pathology (SMD)|Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI (EBY, SMD).
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: de la Monte
mesh_terms: D000818:Animals; D004867:Equipment Design; D006652:Histological Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D008055:Lipids
keywords: Matrix-assisted laser desorption ionization; histology; imaging mass spectrometry; lipids