title: Carbonic anhydrase VI: a novel marker for salivary serous acinar differentiation and its application to discriminate acinic cell carcinoma from mammary analogue secretory carcinoma of the salivary gland.
abstract: OBJECTIVE
  Carbonic anhydrase VI (CA6) is present in serous acinar cells of human salivary glands. The aim of this study was to investigate the diagnostic utility of CA6 in differentiating acinic cell carcinoma (AciCC) from its morphological mimic mammary analogue secretory carcinoma (MASC) of the salivary gland.
  
  
  RESULTS
  CA6 immunostaining was performed in 28 cases of AciCC and 14 cases of MASC. All cases of AciCC showed positive CA6 staining. The staining pattern correlated with the number of serous acinar cells in tumours. All MASCs stained negatively for CA6. The results were compared with those obtained with currently used markers, including DOG1, mammaglobin, S100, and vimentin. CA6 showed sensitivity and specificity as high as those of DOG1 in diagnosing AciCC. CA6 expression was focally observed in basal cell adenoma and in one case of cystadenocarcinoma (1/3), but not in other salivary gland tumours, including mucoepidermoid carcinoma, adenoid cystic carcinoma, salivary duct carcinoma, lymphoepithelial carcinoma, epithelial-myoepithelial carcinoma, and pleomorphic adenoma.
  
  
  CONCLUSIONS
  CA6 is a specific marker for serous acinar cells of salivary glands and AciCC. CA6 has sensitivity and specificity equal to those of DOG1 in the differential diagnosis between AciCC and MASC. A combination of CA6 and DOG1 could be an ideal immunohistochemical panel for AciCC.
authors.affiliation: Department of Pathology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.|Graduate Institute of Pathology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Min-Shu
authors.initials: MS
authors.lastname: Hsieh
authors.affiliation: Department of Pathology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.|Graduate Institute of Pathology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Yung-Ming
authors.initials: YM
authors.lastname: Jeng
authors.affiliation: Graduate Institute of Pathology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Jhuang
authors.affiliation: Department of Anatomical Pathology, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
authors.forename: Yueh-Hung
authors.initials: YH
authors.lastname: Chou
authors.affiliation: Department of Dentistry, Taipei Medical University, Taipei, Taiwan.
authors.forename: Chiao-Ying
authors.initials: CY
authors.lastname: Lin
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D018267:Carcinoma, Acinar Cell / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D000069295:Mammary Analogue Secretory Carcinoma / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D012469:Salivary Glands / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI
keywords: acinic cell carcinoma; carbonic anhydrase VI; mammary analogue secretory carcinoma; salivary gland