title: The acute response of pericytes to muscle-damaging eccentric contraction and protein supplementation in human skeletal muscle.
abstract: Skeletal muscle pericytes increase in quantity following eccentric exercise (ECC) and contribute to myofiber repair and adaptation in mice. The purpose of the present investigation was to examine pericyte quantity in response to muscle-damaging ECC and protein supplementation in human skeletal muscle. Male subjects were divided into protein supplement (WHY; n = 12) or isocaloric placebo (CHO; n = 12) groups and completed ECC using an isokinetic dynamometer. Supplements were consumed 3 times/day throughout the experimental time course. Biopsies were collected prior to (PRE) and 3, 24, 48, and 168 h following ECC. Reflective of the damaging protocol, integrin subunits, including α7, β1A, and β1D, increased (3.8-fold, 3.6-fold and 3.9-fold, respectively, P < 0.01) 24 h post-ECC with no difference between supplements. Pericyte quantity did not change post-ECC. WHY resulted in a small, but significant, decrease in ALP(+) pericytes when expressed as a percentage of myonuclei (CHO 6.8 ± 0.3% vs. WHY 5.8 ± 0.3%, P < 0.05) or per myofiber (CHO 0.119 ± 0.01 vs. WHY 0.098 ± 0.01, P < 0.05). The quantity of myonuclei expressing serum response factor and the number of pericytes expressing serum response factor, did not differ as a function of time post-ECC or supplement. These data demonstrate that acute muscle-damaging ECC increases α7β1 integrin content in human muscle, yet pericyte quantity is largely unaltered. Future studies should focus on the capacity for ECC to influence pericyte function, specifically paracrine factor release as a mechanism toward pericyte contribution to repair and adaptation postexercise.
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and.
authors.forename: Michael
authors.initials: M
authors.lastname: De Lisio
authors.affiliation: Section of Sport Science, Department of Public Health, Aarhus University, Aarhus, Denmark; and.
authors.forename: Jean
authors.initials: J
authors.lastname: Farup
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Sukiennik
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and.
authors.forename: Nicole
authors.initials: N
authors.lastname: Clevenger
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and.
authors.forename: Julian
authors.initials: J
authors.lastname: Nallabelli
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and.
authors.forename: Brett
authors.initials: B
authors.lastname: Nelson
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and.
authors.forename: Kelly
authors.initials: K
authors.lastname: Ryan
authors.affiliation: Section of Sport Science, Department of Public Health, Aarhus University, Aarhus, Denmark; and.
authors.forename: Stine K
authors.initials: SK
authors.lastname: Rahbek
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus, Denmark.
authors.forename: Frank
authors.initials: F
authors.lastname: de Paoli
authors.affiliation: Section of Sport Science, Department of Public Health, Aarhus University, Aarhus, Denmark; and.
authors.forename: Kristian
authors.initials: K
authors.lastname: Vissing
authors.affiliation: Department of Kinesiology and Community Health and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois; and mboppart@illinois.edu.
authors.forename: Marni D
authors.initials: MD
authors.lastname: Boppart
mesh_terms: D019587:Dietary Supplements; D006801:Humans; D008297:Male; D008894:Milk Proteins / Q000008:administration & dosage*; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D020286:Pericytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D026362:Serum Response Factor / Q000378:metabolism; D055815:Young Adult
chemical_list: D008894:Milk Proteins; D026362:Serum Response Factor
keywords: mesenchymal stem cell; muscle damage; muscle regeneration; whey