title: Ganoderma neo-japonicum Imazeki revisited: Domestication study and antioxidant properties of its basidiocarps and mycelia.
abstract: Mushroom cultivation benefits humankind as it deliberately encourages wild mushrooms to be commercially propagated while recycling agricultural wastes. Ganoderma neo-japonicum is a rare polypore mushroom found growing on decaying Schizostachyum brachycladium (a tropical bamboo) clumps in Malaysia. The Malaysian indigenous tribes including the Temuans and Temiars use the basidiocarps of G. neo-japonicum to treat various ailments including diabetes. In this study, the domestication of G. neo-japonicum in artificial logs of different agricultural residues was investigated. Sawdust promoted the mycelia spawn colonisation in the shortest period of 38 ± 0.5 days. However, only sawdust and bamboo dust supported the primodia formation. Complex medium supported mycelium growth in submerged cultures and 27.11 ± 0.43 g/L of mycelia was obtained after 2 weeks of cultivation at 28 °C and 200 rpm. Antioxidant potential in mushroom may be influenced by different cultivation and extraction methods. The different extracts from the wild and cultivated basidiocarps as well as mycelia were then tested for their antioxidant properties. Aqueous and ethanol extracts of mycelia and basidiocarps tested had varying levels of antioxidant activities. To conclude, domestication of wild G. neo-japonicum using agroresidues may ensure a continuous supply of G. neo-japonicum for its medicinal use while ensuring the conservation of this rare species.
authors.affiliation: 1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wee-Cheat
authors.initials: WC
authors.lastname: Tan
authors.affiliation: 1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Umah Rani
authors.initials: UR
authors.lastname: Kuppusamy
authors.affiliation: 1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia [3] Centre of Excellence for Learning and Teaching, UCSI University, Jalan Menara Gading, Taman Connaught, 56000 Kuala Lumpur, Malaysia.
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Phan
authors.affiliation: 1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yee-Shin
authors.initials: YS
authors.lastname: Tan
authors.affiliation: Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Jegadeesh
authors.initials: J
authors.lastname: Raman
authors.affiliation: Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Azliza Mad
authors.initials: AM
authors.lastname: Anuar
authors.affiliation: 1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Vikineswary
authors.initials: V
authors.lastname: Sabaratnam
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis*; D004365:Drugs, Chinese Herbal / Q000138:chemical synthesis*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D032902:Ganoderma / Q000737:chemistry* / Q000254:growth & development*; D008296:Malaysia; D025282:Mycelium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal