title: 2D-DIGE and MALDI TOF/TOF MS analysis reveal that small GTPase signaling pathways may play an important role in cadmium-induced colon cell malignant transformation.
abstract: Cadmium is a toxic heavy metal present in the environment and in industrial materials. Cadmium has demonstrated carcinogenic activity that induces cell transformation, but how this occurs is unclear. We used 2D-DIGE and MALDI TOF/TOF MS combined with bioinformatics and immunoblotting to investigate the molecular mechanism of cadmium transformation. We found that small GTPases were critical for transformation. Additionally, proteins involved in mitochondrial transcription, DNA repair, and translation also had altered expression patterns in cadmium treated cells. Collectively, our results suggest that activation of small GTPases contributes to cadmium-induced transformation of colon cells.
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China; Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China. Electronic address: lujian@ujs.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China; Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Respiration Medicine, Changzhou No.2 People's Hospital, Changzhou 213003, China.
authors.forename: Jianzhou
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China; Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhuyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Rongzhu
authors.initials: R
authors.lastname: Lu
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Shi
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China. Electronic address: tuzg_ujs@ujs.edu.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Tu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019256:Cadmium Chloride / Q000633:toxicity*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D019295:Computational Biology; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D008297:Male; D008819:Mice, Nude; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D009368:Neoplasm Transplantation; D015398:Signal Transduction / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D047368:Tumor Burden / Q000187:drug effects; D057825:Two-Dimensional Difference Gel Electrophoresis; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: C066737:RHOA protein, human; D020559:Monomeric GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D019256:Cadmium Chloride
keywords: 2D-DIGE; Cadmium; Colon cell; MALDI TOF/TOF MS; Small GTPase; Transformation