title: Lipopeptide-Coated Iron Oxide Nanoparticles as Potential Glycoconjugate-Based Synthetic Anticancer Vaccines.
abstract: Although iron oxide magnetic nanoparticles (NPs) have been widely utilized in molecular imaging and drug delivery studies, they have not been evaluated as carriers for glycoconjugate-based anticancer vaccines. Tumor-associated carbohydrate antigens (TACAs) are attractive targets for the development of anticancer vaccines. Due to the weak immunogenicity of these antigens, it is highly challenging to elicit strong anti-TACA immune responses. With their high biocompatibilities and large surface areas, magnetic NPs were synthesized for TACA delivery. The magnetic NPs were coated with phospholipid-functionalized TACA glycopeptides through hydrophobic-hydrophobic interactions without the need for any covalent linkages. Multiple copies of glycopeptides were presented on NPs, potentially leading to enhanced interactions with antibody-secreting B cells through multivalent binding. Mice immunized with the NPs generated strong antibody responses, and the glycopeptide structures important for high antibody titers were identified. The antibodies produced were capable of recognizing both mouse and human tumor cells expressing the glycopeptide, resulting in tumor cell death through complement-mediated cytotoxicities. These results demonstrate that magnetic NPs can be a new and simple platform for multivalently displaying TACA and boosting anti-TACA immune responses without the need for a typical protein carrier.
authors.affiliation: Department of Chemistry, Michigan State University, Chemistry Building, Room 426, 578 South Shaw Lane, East Lansing, Michigan 48824, United States.
authors.forename: Suttipun
authors.initials: S
authors.lastname: Sungsuwan
authors.affiliation: Department of Chemistry, Michigan State University, Chemistry Building, Room 426, 578 South Shaw Lane, East Lansing, Michigan 48824, United States.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Chemistry, Michigan State University, Chemistry Building, Room 426, 578 South Shaw Lane, East Lansing, Michigan 48824, United States.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000276:immunology; D001672:Biocompatible Materials / Q000737:chemistry; D019496:Cancer Vaccines / Q000276:immunology*; D003167:Complement Activation; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D000067493:Dynamic Light Scattering; D005290:Ferric Compounds / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D006020:Glycopeptides / Q000276:immunology; D006801:Humans; D055666:Lipopeptides / Q000737:chemistry*; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D018396:Mucin-1 / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D001672:Biocompatible Materials; D019496:Cancer Vaccines; D005290:Ferric Compounds; D006001:Glycoconjugates; D006020:Glycopeptides; D055666:Lipopeptides; D058185:Magnetite Nanoparticles; D018396:Mucin-1; D014614:Vaccines, Synthetic; C000499:ferric oxide
keywords: MUC1; cancer vaccine; glycopeptide; immunology; magnetic nanoparticles