title: Recent advances in biosensors based on enzyme inhibition.
abstract: Enzyme inhibitors like drugs and pollutants are closely correlated to human and environmental health, thus their monitoring is of paramount importance in analytical chemistry. Enzymatic biosensors represent cost-effective, miniaturized and easy to use devices; particularly biosensors based on enzyme inhibition are useful analytical tools for fast screening and monitoring of inhibitors. The present review will highlight the research carried out in the last 9 years (2006-2014) on biosensors based on enzyme inhibition. We underpin the recent advances focused on the investigation in new theoretical approachs and in the evaluation of biosensor performances for reversible and irreversible inhibitors. The use of nanomaterials and microfluidic systems as well as the applications of the various biosensors in real samples is critically reviewed, demonstrating that such biosensors allow the development of useful devices for a fast and reliable alarm system.
authors.affiliation: Faculty of Sciences and Techniques, University Hassan II of Casablanca, Morocco. Electronic address: azizamine@yahoo.fr.
authors.forename: A
authors.initials: A
authors.lastname: Amine
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi "INBB", Viale Medaglie d'Oro 305, 00136 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Arduini
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi "INBB", Viale Medaglie d'Oro 305, 00136 Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Moscone
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi "INBB", Viale Medaglie d'Oro 305, 00136 Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Palleschi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006801:Humans
chemical_list: D004785:Environmental Pollutants; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized
keywords: Analytical applications; Biosensor; Enzyme inhibition; Nanomaterials