title: Seminal Corynebacterium strains in infertile men with and without leucocytospermia.
abstract: This study aimed to identify seminal Corynebacterium strains in infertile men with and without leucocytospermia. Semen samples from 60 infertile men were allocated into two equal groups: semen samples with leucocytospermia and semen samples without leucocytospermia. Semen culture for Corynebacterium species was carried out on Columbia agar medium confirmed by Gram-stained film and biochemical tests followed by analytical profile index biotyping and antibiotic susceptibility. Bacterial isolates were detected in 20/60 semen cultures (33.3%) as Corynebacteria, Staphylococci, Alpha haemolytic streptococci and E. coli. In all, 12/60 (20%) had Corynebacterium positive semen culture, whereas C. seminal was the major isolated species followed by C. amycolatum, C. jekium and C. urealyticum. There was nonsignificant difference between patients with/without Corynebacterium positive culture regarding sperm concentration and normal sperm morphology; however, in positive cultures sperm motility was significantly lower compared with negative cultures. Antimicrobial sensitivity among Corynebacteria strains was highest for vancomycin, rifampicin then imipenem, ampicillin + sulbactam, ciprofloxacin. It is concluded that positive semen cultures for different Corynebacteria species were demonstrated in infertile men, whereas Corynebacterium seminale was the most common isolated species. Vancomycin, rifampicin then imipenem and ampicillin + sulbactam are recommended as sensitive antibiotics.
authors.affiliation: Division of Andrology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Mashaly
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: D T
authors.initials: DT
authors.lastname: Masallat
authors.affiliation: Division of Andrology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: A A
authors.initials: AA
authors.lastname: Elkholy
authors.affiliation: Division of Andrology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: I A
authors.initials: IA
authors.lastname: Abdel-Hamid
authors.affiliation: Department of Andrology & Sexology and STIs, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: T
authors.initials: T
authors.lastname: Mostafa
mesh_terms: D000328:Adult; D003352:Corynebacterium / Q000302:isolation & purification*; D003430:Cross-Sectional Studies; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000382:microbiology*; D008297:Male; D012661:Semen / Q000382:microbiology*; D013076:Sperm Count; D013081:Sperm Motility; D055815:Young Adult
keywords: Antibiotics; Corynebacteria; leucocytospermia; male infertility; semen