title: Data in support of peptidomic analysis of spermatozoa during epididymal maturation.
abstract: The final differentiation of the male germ cell occurs in the epididymal duct where the spermatozoa develop the ability to be motile and fertilize an ovum. Understanding of these biological processes is the key to understanding and controlling male fertility. Comparative studies between several epididymal maturation states could be an informative approach to finding sperm modifications related to maturation and fertility. Here we show the data from differential peptidomic/proteomic analyses on spermatozoa isolated from 4 epididymal regions (immature to mature stage) using a profiling approach based on MALDI-TOF mass spectrometry and, combined to top-down MS in order to identify peptidoforms and proteoforms. By this way, 172m/z peaks ranging between 2 and 20 kDa were found to be modified during maturation of sperm. A total of 62m/z were identified corresponding to 32 different molecular species. The interpretation of these data can be found in the research article published by Labas and colleagues in the Journal of Proteomics in 2014 [1].
authors.affiliation: INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France ; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France ; CNRS, UMR7247, F-37380 Nouzilly, France ; Université François Rabelais de Tours, F-37000 Tours, France ; IFCE, F-37380 Nouzilly, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Labas
authors.affiliation: INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France ; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France ; CNRS, UMR7247, F-37380 Nouzilly, France ; Université François Rabelais de Tours, F-37000 Tours, France ; IFCE, F-37380 Nouzilly, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Spina
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France ; CNRS, UMR7247, F-37380 Nouzilly, France ; Université François Rabelais de Tours, F-37000 Tours, France ; IFCE, F-37380 Nouzilly, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Belleannee
authors.affiliation: INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France ; INRA, UMR1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France ; Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France.
authors.forename: Ana-Paula
authors.initials: AP
authors.lastname: Teixeira-Gomes
authors.affiliation: INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France ; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France ; CNRS, UMR7247, F-37380 Nouzilly, France ; Université François Rabelais de Tours, F-37000 Tours, France ; IFCE, F-37380 Nouzilly, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gargaros
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France ; CNRS, UMR7247, F-37380 Nouzilly, France ; Université François Rabelais de Tours, F-37000 Tours, France ; IFCE, F-37380 Nouzilly, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Dacheux
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France ; CNRS, UMR7247, F-37380 Nouzilly, France ; Université François Rabelais de Tours, F-37000 Tours, France ; IFCE, F-37380 Nouzilly, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Dacheux
keywords: Epididymis; Maturation; Peptidome; Spermatozoa