title: Enzymatic selective synthesis of 1,3-DAG based on deep eutectic solvent acting as substrate and solvent.
abstract: In this study, enzymatic selective esterification of oleic acid with glycerol based on deep eutectic solvent acting as substrate and solvent was studied. As choline chloride (ChCl) or betaine can effectively change the chemical reaction characteristics of glycerol when they are mixed with a certain molar ratio of glycerol, several factors crucial to the lipase catalytic esterification of glycerol with oleic acid was investigated. Results showed that, betaine had more moderate effects than ChCl on the lipase, and water content had an important influence of the esterification and the enzyme selectivity. Significant changes of the glyceride compositions and enzyme selectivity were found in ChCl adding system compared with pure glycerol system; optimum accumulation of DAG especially 1,3-DAG because of the eutectic effect of ChCl was found in this system. Furthermore, in a model 1,3-DAG esterification synthesis system catalyzed by Novozym 435, high content (42.9 mol%) of the 1,3-DAG could be obtained in ChCl adding system within 1 h.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Chao-Xi
authors.initials: CX
authors.lastname: Zeng
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Sui-Jian
authors.initials: SJ
authors.lastname: Qi
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Rui-Pu
authors.initials: RP
authors.lastname: Xin
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China. yonghw@scut.edu.cn.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Wang
mesh_terms: D004075:Diglycerides / Q000138:chemical synthesis* / Q000737:chemistry; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D012997:Solvents / Q000737:chemistry
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D012997:Solvents; C093667:Novozyme 435; D008049:Lipase
keywords: 1,3-Diacylglycerol; Deep eutectic solvents; Esterification; Lipase