title: Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: A case of melioidosis with a mycotic aneurysm is reported. The blood and tissue isolates were identified as three different species of Burkholderia using the automated identification systems, VITEK 2 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The isolate was confirmed as Burkholderia pseudomallei by 16S rRNA sequencing. The typical features of the Gram staining of colonies and 16S rRNA sequencing can be useful to identify B. pseudomallei.
authors.affiliation: Department of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of Korea.
authors.forename: Hee Ryeong
authors.initials: HR
authors.lastname: Jang
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of Korea.
authors.forename: Chung Won
authors.initials: CW
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Pusan National University Hospital, Seogu, Busan, Republic of Korea.
authors.forename: Soon Jung
authors.initials: SJ
authors.lastname: Ok
authors.affiliation: Department of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of Korea.
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of Korea.
authors.forename: Mi Ju
authors.initials: MJ
authors.lastname: Bae
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of Korea.
authors.forename: Seunghwan
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, Pusan National University Hospital, Seogu, Busan, Republic of Korea; Biomedical Research Institute, Pusan National University Hospital, Seogu, Busan, Republic of Korea.
authors.forename: Jongyoun
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of Korea; Biomedical Research Institute, Pusan National University Hospital, Seogu, Busan, Republic of Korea. Electronic address: kyehyungs@gmail.com.
authors.forename: Kye-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000785:Aneurysm, Infected / Q000175:diagnosis*; D016957:Burkholderia pseudomallei / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000175:diagnosis*; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Burkholderia pseudomallei; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Melioidosis; RNA sequence analysis