title: A giant local interneuron modulates the rhythmic activities of the antennal lobe in Pupae Drosophila.
abstract: In Drosophila, olfaction is tightly related to feeding and reproduction. There are three classes of neurons forming synapses in the olfactory circuit: the olfactory receptor neurons (ORNs), projection neurons (PNs), and local interneurons (LNs). Here, we showed that giant local interneurons named GLNs, which were different from the classical neurons in the olfactory circuits, displayed distinctive rhythmic activities in the dorsolateral side of antennal lobe (AL) in Drosophila Pupae. Anatomically, GLNs were much larger than ipsilateral LNs and extended arborizations throughout the AL. Electrophysiologically, GLN exhibited typical 4-phased rhythmic spontaneous membrane activities, and the surrounding cells were dye-coupled when biocytin was injected into the cell body of GLN. Our study demonstrated that spontaneous activities of GLNs correlated with that of LNs and PNs. After the GLNs were damaged, the membrane activities of ipsilateral LNs and PNs became smaller, but faster. By depressing the firing frequencies of PNs and LNs, GLNs modulated the synchronization of AL and might play an important role as a "modulator" in the local circuit.
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Mintong
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Ran
authors.affiliation: Affiliated Psychiatric Hospital of Guangzhou, Medical College, Guangzhou 510080, PR China.
authors.forename: Xicui
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Yulou
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Xutian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China.
authors.forename: Yingcheng
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: hhxu@scau.edu.cn.
authors.forename: Hanhong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China. Electronic address: gu_huaiyu@yahoo.com.
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Gu
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000502:physiology; D004330:Drosophila / Q000502:physiology*; D007395:Interneurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010507:Periodicity; D011679:Pupa / Q000502:physiology
keywords: Antennal lobe; Drosophila Pupae; Giant local interneuron; Spontaneous activity; Synchronization