title: Fish Oil Enhances T Cell Function and Tumor Infiltration and Is Correlated With a Cancer Prevention Effect in HER-2/neu But Not PyMT Transgenic Mice.
abstract: Few studies have explored the effects of omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementation on immune modulation in murine models of mammary carcinogenesis. HER-2/neu and PyMT mice were randomized to 2 dietary interventions: AIN-93G-based diet with 1) 11% of diet (per gram weight) as corn oil (CO) or 2) 10% of diet as menhaden fish oil plus 1% of diet as corn oil (FO). FO significantly reduced the incidence and multiplicity of tumors (P < 0.001) in HER-2/neu, but not PyMT mice. FO-fed mice had significantly larger splenocyte counts than CO-fed mice in both the HER-2/neu and PyMT models; and in both models this was comprised of an increase in most cell types, including Gr-1(+)/CD11b(+) cells. T cells from FO-fed HER-2/neu mice produced significantly more interleukin-2 (P = 0.004) and interferon-γ (P = 0.012) in response to in vitro stimulation with anti-CD3 (0.5 µg/ml). Lastly, FO-fed HER-2/neu mice had significantly more tumor immune infiltrates than CO-fed mice, including NK1.1(+), F4/80(+), and Gr-1(+)/CD11b(+) cells (P ≤ 0.05). Greater Th1 cytokine production and significantly more tumor immune infiltrates in FO-fed Her2/neu mice may account for the cancer prevention effect of fish oil in this model.
authors.affiliation: a Department of Nutritional Sciences , Pennsylvania State University, University Park , Pennsylvania , USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Turbitt
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Black
authors.forename: Shawntawnee D
authors.initials: SD
authors.lastname: Collins
authors.forename: Huicui
authors.initials: H
authors.lastname: Meng
authors.forename: Haifang
authors.initials: H
authors.lastname: Xu
authors.forename: Sharlene
authors.initials: S
authors.lastname: Washington
authors.forename: Cesar
authors.initials: C
authors.lastname: Aliaga
authors.forename: Karam
authors.initials: K
authors.lastname: El-Bayoumy
authors.forename: Andrea
authors.initials: A
authors.lastname: Manni
authors.forename: Connie J
authors.initials: CJ
authors.lastname: Rogers
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003314:Corn Oil / Q000494:pharmacology; D004195:Disease Models, Animal; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009369:Neoplasms / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007376:Interleukin-2; C033266:Menhaden oil; D003314:Corn Oil; D007371:Interferon-gamma