title: Physical exercise prevents motor disorders and striatal oxidative imbalance after cerebral ischemia-reperfusion.
abstract: Stroke is the third most common cause of death worldwide, and most stroke survivors present some functional impairment. We assessed the striatal oxidative balance and motor alterations resulting from stroke in a rat model to investigate the neuroprotective role of physical exercise. Forty male Wistar rats were assigned to 4 groups: a) control, b) ischemia, c) physical exercise, and d) physical exercise and ischemia. Physical exercise was conducted using a treadmill for 8 weeks. Ischemia-reperfusion surgery involved transient bilateral occlusion of the common carotid arteries for 30 min. Neuromotor performance (open-field and rotarod performance tests) and pain sensitivity were evaluated beginning at 24 h after the surgery. Rats were euthanized and the corpora striata was removed for assay of reactive oxygen species, lipoperoxidation activity, and antioxidant markers. Ischemia-reperfusion caused changes in motor activity. The ischemia-induced alterations observed in the open-field test were fully reversed, and those observed in the rotarod test were partially reversed, by physical exercise. Pain sensitivity was similar among all groups. Levels of reactive oxygen species and lipoperoxidation increased after ischemia; physical exercise decreased reactive oxygen species levels. None of the treatments altered the levels of antioxidant markers. In summary, ischemia-reperfusion resulted in motor impairment and altered striatal oxidative balance in this animal model, but those changes were moderated by physical exercise.
authors.affiliation: Grupo de Pesquisa em Fisiologia, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: P M
authors.initials: PM
authors.lastname: Sosa
authors.affiliation: Grupo de Pesquisa em Neuromecânica Aplicada, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: H L
authors.initials: HL
authors.lastname: Schimidt
authors.affiliation: Grupo de Pesquisa em Fisiologia, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: C
authors.initials: C
authors.lastname: Altermann
authors.affiliation: Grupo de Pesquisa em Fisiologia, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: A S
authors.initials: AS
authors.lastname: Vieira
authors.affiliation: Laboratório de Biotecnologia da Reprodução, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: F W S
authors.initials: FW
authors.lastname: Cibin
authors.affiliation: Grupo de Pesquisa em Neuromecânica Aplicada, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: F P
authors.initials: FP
authors.lastname: Carpes
authors.affiliation: Grupo de Pesquisa em Fisiologia, Universidade Federal do Pampa, Uruguaiana, RS, BR.
authors.forename: P B
authors.initials: PB
authors.lastname: Mello-Carpes
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D000068079:Motor Disorders / Q000209:etiology / Q000517:prevention & control*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D010146:Pain / Q000503:physiopathology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000032:analysis; D015427:Reperfusion Injury / Q000150:complications*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione