title: Inhibitory Effects of Purple Sweet Potato Leaf Extract on the Proliferation and Lipogenesis of the 3T3-L1 Preadipocytes.
abstract: Purple sweet potato leaves (PSPLs) are healthy vegetable that is rich in anti-oxidants. A solution of boiling water extract of PSPL (PSPLE) is believed to be able to prevent obesity and metabolic syndrome in the countryside of Taiwan, but its efficacy has not yet been verified. The purpose of this study was to investigate the possible anti-adipogenesis effect of PSPLE in vitro. PSPLE was used to treat the 3T3-L1 cells, and the effects on cell proliferation and adipogenesis were investigated. The results showed that PSPLE caused a dose-dependent decrease in the cell proliferation of 3T3-L1 preadipocytes, but did not alter the cell viability. In addition, PSPLE induced ERK inactivation in the 3T3-L1 preadipocytes. Furthermore, pre-treatment of confluent 3T3-L1 cells with PSPLE led to reduced lipid accumulation in differentiated 3T3-L1 cells. The inhibition of lipogenesis could result from the PSPLE-induced down-regulation of the expression of the C/EBPα and SREBP-1 transcription factors during 3T3-L1 adipocyte differentiation. These results suggest that PSPLE not only inhibits cell proliferation at an early stage but also inhibits adipogenesis at a later stage of the differentiation program.
authors.affiliation: Department of Biological Science and Technology, Taiwan.
authors.forename: Shou-Lun
authors.initials: SL
authors.lastname: Lee
authors.affiliation: Department of Anesthesiology, Chang Bing Show Chwan Memorial Hospital, Changhua County 50544, Taiwan.
authors.forename: Hsien-Kuang
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Zhongli, Taoyuan 32023, Taiwan.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Chin
authors.affiliation: Department of Biological Science and Technology, Taiwan.
authors.forename: Ssu-Chieh
authors.initials: SC
authors.lastname: Tu
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Zhongli, Taoyuan 32023, Taiwan.
authors.forename: Ming-Hsun
authors.initials: MH
authors.lastname: Kuo
authors.affiliation: Department of Biological Science and Technology, Taiwan.
authors.forename: Ming-Ching
authors.initials: MC
authors.lastname: Kao
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.|Chinese Medicine Research and Development Center, Taiwan.|Center for Molecular Medicine, China Medical University Hospital, Taichung 40447, Taiwan.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D027723:Ipomoea batatas / Q000737:chemistry*; D050155:Lipogenesis / Q000187:drug effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D024821:Metabolic Syndrome / Q000517:prevention & control; D051379:Mice; D009765:Obesity / Q000517:prevention & control; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D014867:Water
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D010936:Plant Extracts; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D014867:Water
keywords: 3T3-L1 Cells; Anti-adipogenesis; Anti-proliferation; C/EBPα;SREBP-1; PPARγ