title: Stability of anthocyanins- and anthocyanidins-enriched extracts, and formulations of fruit pulp of Eugenia jambolana ('jamun').
abstract: The fruit pulp of Eugenia jambolana (jamun) is a rich source of anthocyanins (ACs). The purpose of the present study was to assess the effect of various physical and chemical factors on the stability of ACs and anthocyanidins (ACdn) in the crude, anthocyanins-enriched extract (ACs-EEX), anthocyanins-enriched sephadex extract (ACs-EES) and anthocyanidins-enriched extract (ACdn-EEX). ACs and ACdn contents were analyzed using a stability indicating HPLC analytical method. The ACs content reduced to 86.4% (crude extract), 60.9% (ACs-EEX), 36.0% (ACs-EES), 64.8% (ACs-EEX tablet), and 71.7% (ACs-EEX capsules) after 1 year at 5 °C. The ACdn content reduced to 83.1% (ACdn-EEX), 90.1% (ACdn-EEX tablet) and 93.8% (ACdn-EEX capsules) after 1 year at 5 °C. ACs and ACdn showed lesser degradation at low pH and higher degradation at high H2O2 concentration. The thermal degradation products of ACs were identified and quantified.
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160062, India.
authors.forename: Ram Jee
authors.initials: RJ
authors.lastname: Sharma
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA; Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Gupta
authors.affiliation: Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160062, India.
authors.forename: Saranjit
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160062, India.
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Bansal
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160062, India. Electronic address: ipsingh@niper.ac.in.
authors.forename: Inder Pal
authors.initials: IP
authors.lastname: Singh
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts
keywords: Anthocyanidins; Anthocyanins; Degradation products; Eugenia jambolana; HPLC-PDA; Stability study