title: The up-regulation of miR-300 in gastric cancer and its effects on cells malignancy.
abstract: OBJECTIVE
  In this study, we investigated the role of miR-300 in regulating cell proliferation and invasion of gastric cancer cells.
  
  
  METHODS
  MicroRNA and protein expression patterns were compared between gastric cancer tissue and normal tissue and between two different prognostic groups. The up-regulation of miR-300 was confirmed by real-time reverse transcription polymerase chain reaction and its expression was analyzed in AGS gastric cancer cells.
  
  
  RESULTS
  We observed that miR-300 expression was frequently and dramatically up-regulated in human gastric cancer tissues and cell lines compared with the matched adjacent normal tissues and cells. We further showed that transient and stable over-expression of miR-300 could promote cell proliferation and cell cycle progression. Moreover, p53, a key inhibitor of cell cycle, was verified as a direct target of miR-300, suggesting that miR-300 might promote gastric cancer cell proliferation and invasion by increasing p53 expression.
  
  
  CONCLUSIONS
  Our findings indicated that miR-300 up-regulation might exert some sort of antagonistic function by targeting p53 in gastric cancer cell proliferation during gastric tumorigenesis.
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Colorectal & Anal Surgery China-Japan Union Hospital of Jilin University China.
authors.forename: Tongjun
authors.initials: T
authors.lastname: Liu
keywords: AGS; gastric cancer; miR-300; p53; target therapy