title: Analysis of ATP and AMP binding to a DNA aptamer and its imidazole-tethered derivatives by surface plasmon resonance.
abstract: Imidazole was tethered to the C5 position of thymine in an ATP-binding DNA aptamer with two types of linkers, and the affinities of each aptamer for ATP and AMP were determined by surface plasmon resonance measurements. The imidazole-tethered aptamers exhibited higher affinity for ATP, almost independently of the linker structure or the modification site.
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Satoshi
authors.initials: S
authors.lastname: Katsube
authors.forename: Junpei
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
mesh_terms: D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D020349:Surface Plasmon Resonance
chemical_list: D052157:Aptamers, Nucleotide; D007093:Imidazoles; D000249:Adenosine Monophosphate; C029899:imidazole; D000255:Adenosine Triphosphate; D004247:DNA