title: Interplay of the PtsN (EIIA(Ntr)) protein of Pseudomonas putida with its target sensor kinase KdpD.
abstract: The nitrogen phosphotransferase system (PTS(Ntr) ) of Pseudomonas putida is a multi-component regulatory device that participates in controlling a variety of physiological processes in a post-translational fashion. A general survey of genes regulated by PtsN exposed transcription of the kdpFABC operon is most conspicuously affected. Measurements of kdpFp promoter activity in different pts mutants showed that PtsN is responsible for repression of kdpFABC transcription. This effect could be assigned mainly to PtsN∼P, depending on the external K(+) concentration. Bacterial two-hybrid assays demonstrated that kdpFp regulation is implemented through direct interaction of the PtsN protein with the sensor kinase KdpD of the KdpD/KdpE two-component system. Interaction between KdpD and PtsN was detectable with a PtsN variant that imitates the non-phosphorylated form as well as with a PtsN type mimicking the phosphorylated form of PtsN. These results raise a regulatory scenario in which the Kdp system is regulated by the action of PtsN through direct interaction with the sensor kinase KdpD, and the outcome of such an interaction depends on the phosphorylation state of PtsN as well as on the external K(+) concentration.
authors.affiliation: Fachgebiet für Systembiotechnologie, Technische Universität München, Garching, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Deuschle
authors.affiliation: Fachgebiet für Systembiotechnologie, Technische Universität München, Garching, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Limbrunner
authors.affiliation: Fachgebiet für Systembiotechnologie, Technische Universität München, Garching, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Rother
authors.affiliation: Fachgebiet für Systembiotechnologie, Technische Universität München, Garching, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wahler
authors.affiliation: Escuela de Química & CIPRONA, Universidad de Costa Rica, San José, Costa Rica.|Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Chavarría
authors.affiliation: Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.
authors.forename: V
authors.initials: V
authors.lastname: de Lorenzo
authors.affiliation: Fachgebiet für Systembiotechnologie, Technische Universität München, Garching, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Kremling
authors.affiliation: Fachgebiet für Systembiotechnologie, Technische Universität München, Garching, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Pflüger-Grau
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C423631:PtsN protein, Pseudomonas putida; D011494:Protein Kinases; C075616:kdpD protein, Bacteria; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D011188:Potassium