title: In Vivo Longitudinal (1)H MRS Study of Transgenic Mouse Models of Prion Disease in the Hippocampus and Cerebellum at 14.1 T.
abstract: In vivo (1)H MR spectroscopy allows the non invasive characterization of brain metabolites and it has been used for studying brain metabolic changes in a wide range of neurodegenerative diseases. The prion diseases form a group of fatal neurodegenerative diseases, also described as transmissible spongiform encephalopathies. The mechanism by which prions elicit brain damage remains unclear and therefore different transgenic mouse models of prion disease were created. We performed an in vivo longitudinal (1)H MR spectroscopy study at 14.1 T with the aim to measure the neurochemical profile of Prnp -/- and PrPΔ32-121 mice in the hippocampus and cerebellum. Using high-field MR spectroscopy we were able to analyze in details the in vivo brain metabolites in Prnp -/- and PrPΔ32-121 mice. An increase of myo-inositol, glutamate and lactate concentrations with a decrease of N-acetylaspartate concentrations were observed providing additional information to the previous measurements.
authors.affiliation: Centre d'Imagerie Biomedicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. cristina.cudalbu@epfl.ch.
authors.forename: Cristina
authors.identifier: http://orcid.org/0000-0003-4582-2465
authors.initials: C
authors.lastname: Cudalbu
authors.affiliation: Laboratory for Functional and Metabolic Imaging (LIFMET), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors.forename: Melanie
authors.initials: M
authors.lastname: Craveiro
authors.affiliation: Centre d'Imagerie Biomedicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mlynárik
authors.affiliation: Institute of Neuropathology, University Hospital of Zurich, Zurich, Switzerland.
authors.forename: Juliane
authors.initials: J
authors.lastname: Bremer
authors.affiliation: Institute of Neuropathology, University Hospital of Zurich, Zurich, Switzerland.
authors.forename: Adriano
authors.initials: A
authors.lastname: Aguzzi
authors.affiliation: Centre d'Imagerie Biomedicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.|Laboratory for Functional and Metabolic Imaging (LIFMET), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.|Departments of Radiology, Universities of Lausanne and Geneva, Lausanne, Switzerland.
authors.forename: Rolf
authors.initials: R
authors.lastname: Gruetter
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001923:Brain Chemistry / Q000235:genetics; D002531:Cerebellum / Q000473:pathology*; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000473:pathology*; D007294:Inositol / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D017096:Prion Diseases / Q000473:pathology*; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics
chemical_list: D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D001224:Aspartic Acid; D019344:Lactic Acid; D018698:Glutamic Acid; D007294:Inositol; C000179:N-acetylaspartate
keywords: Brain metabolites; In vivo proton magnetic resonance spectroscopy; Mouse brain; Prion disease