title: The Rapid Screening of Triazophos Residues in Agricultural Products by Chemiluminescent Enzyme Immunoassay.
abstract: A highly sensitive chemiluminescent enzyme immunoassay (CLEIA) method was developed in this study for efficient screening of triazophos residues in a large number of samples. Based on the maximum residue limits (MRLs) set by China and CAC for triazophos in different agro-products, the representative apple, orange, cabbage, zucchini, and rice samples were selected as spiked samples, and the triazophos at the concentrations of the MRL values were spiked to blank samples. Subsequently, the five samples with the spiked triazophos standard were measured by CLEIA 100 times, and the detection results indicated that the correction factors of the apple, orange, cabbage, zucchini, and rice were determined as 0.79, 0.66, 0.85, 0.76, and 0.91, respectively. In this experiment, 1500 real samples were detected by both the CLEIA and the GC-MS methods. With the GC-MS method, 1462 samples were identified as negative samples and 38 samples as positive samples. Based on the correction factors, the false positive rate of the CLEIA method was 0.13%, and false negative rate was 0. The results showed that the established CLEIA method could be used to screen a large number of real samples.
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Du
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shao
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Jin
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Lufei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: She
authors.affiliation: Key Laboratory for Agro-Products Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000383:Agriculture; D002681:China; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D057166:High-Throughput Screening Assays / Q000379:methods*; D007124:Immunoenzyme Techniques / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D063086:Organothiophosphates / Q000032:analysis*; D014230:Triazoles / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D063086:Organothiophosphates; D014230:Triazoles; C012584:triazophos