title: Going Viral with Fluorescent Proteins.
abstract: Many longstanding questions about dynamics of virus-cell interactions can be answered by combining fluorescence imaging techniques with fluorescent protein (FP) tagging strategies. Successfully creating a FP fusion with a cellular or viral protein of interest first requires selecting the appropriate FP. However, while viral architecture and cellular localization often dictate the suitability of a FP, a FP's chemical and physical properties must also be considered. Here, we discuss the challenges of and offer suggestions for identifying the optimal FPs for studying the cell biology of viruses.
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Lindsey M
authors.initials: LM
authors.lastname: Costantini
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA erik-lee.snapp@einstein.yu.edu.
authors.forename: Erik L
authors.initials: EL
authors.lastname: Snapp
mesh_terms: D060833:Cellular Microenvironment; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D061848:Optical Imaging / Q000379:methods*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000378:metabolism*; D014773:Virology / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D014760:Viral Fusion Proteins