title: Correlation of human epidermal growth factor receptor protein expression and colorectal cancer.
abstract: OBJECTIVE
  To investigate the correlation between human epidermal growth factor receptor (HER-2) protein expression and colorectal cancer (CRC) using a case-control study and meta-analysis.
  
  
  METHODS
  Tumor tissue specimens from 162 CRC patients were selected for the case group. Fifty cases were randomly selected, and normal CRC tissue at least 10 cm away from the tumor margins of these cases was used to generate the control group. The expression of the HER-2 protein in the 162 CRC tissue samples and the 50 adjacent normal mucosa tissue samples was detected via immunohistochemistry. The experimental data were analyzed using SPSS 18.0 software, and R software version 3.1.0 was utilized for further verification.
  
  
  RESULTS
  The expression of HER-2 protein in the 162 CRC tissue samples was significantly higher than in the normal tissue specimens. The data showed that the expression of HER-2 in CRC was related to the Dukes' stage, the depth of invasion and lymph node metastasis. The HER-2-positive patients had lower 3- and 5-year OS rates than the HER-2-negative patients, but there was no significant difference. However, there was a statistically significant difference in the 3- and 5-year disease-free survival (DFS) rates of HER-2-positive and HER-2-negative patients. The results of the meta-analysis showed that the expression of HER-2 in CRC patients was statistically significantly increased over that of healthy people. The 3-year DFS rate in HER-2-positive patients was markedly lower than that in HER-2-negative patients.
  
  
  CONCLUSIONS
  Down-regulation of HER-2 expression might be a dependable strategy for CRC therapy.
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Shen
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Ma
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Tan
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Yi-Tong
authors.initials: YT
authors.lastname: Guo
authors.affiliation: Wen-Juan Yang, Zhi-Gang Tan, Yan Song, Yi-Tong Guo, Department of Emergency, Jinan Central Hospital affiliated Shandong University, Jinan 250013, Shandong Province, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D018572:Disease-Free Survival; D066246:ErbB Receptors / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D016017:Odds Ratio; D011237:Predictive Value of Tests; D012307:Risk Factors; D016019:Survival Analysis; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Biomarker; Case-control study; Colorectal cancer; Human epidermal growth factor receptor; Immunohistochemistry; Meta-analysis; Therapy