title: Role of TRPV1 in the Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes.
abstract: Cytosolic Ca2+ ([Ca2+]i) is an important signal that regulates cardiomyocyte differentiation during cardiogenesis. TRPV1 is a Ca2+-permeable channel that is expressed in cardiomyocytes. In the present study, we utilized mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs) as a model to investigate the functional role of TRPV1 in cardiomyocyte differentiation. Induction of embryonic stem cells into cardiomyocytes was achieved using embryoid body (EB)-based differentiation method. Quantitative PCRs showed an increased TRPV1 expression during the differentiation process. In [Ca2+]i measurement study, application of TRPV1 agonists, capsaicin and camphor, elicited a [Ca2+]i rise in mESC-CMs, the effect of which was abolished by TRPV1-shRNA. In functional study, treatment of EBs with TRPV1 antagonists (capsazepine and SB366791) and TRPV1-shRNA reduced the size of the EBs and decreased the percentage of spontaneously beating EBs. TRPV1 antagonists and TRPV1-shRNA also suppressed the expression of cardiomyocyte marker genes, including cardiac actin, c-TnT, c-TnI, and α-MHC. Taken together, this study demonstrated an important functional role of TRPV1 channels in the differentiation of mESCs into cardiomyocytes.
authors.affiliation: Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.affiliation: School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Zenghua
authors.initials: Z
authors.lastname: Qi
authors.affiliation: Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Chun-Kit
authors.initials: CK
authors.lastname: Wong
authors.affiliation: School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Chun
authors.initials: C
authors.lastname: So
authors.affiliation: School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Iek-Chi
authors.initials: IC
authors.lastname: Lo
authors.affiliation: Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Yao
authors.affiliation: School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China; Partner State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kong, Hong Kong, China; Centre of Novel Biomaterials, Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Suk-Ying
authors.initials: SY
authors.lastname: Tsang
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D050916:TRPV Cation Channels / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse