title: Identification and Characterization of Homing Peptides Using In Vivo Peptide Phage Display.
abstract: Each normal organ and pathological condition expresses a distinct set of molecules on their vasculature. These molecular signatures have been efficiently profiled using in vivo phage display. Several peptides homing to tumor blood vessels, lymphatic vessels, and/or tumor cells as well as to various normal organs have been isolated using this method. The in vivo screening of phage libraries has also revealed novel tissue-specific biomarkers of the normal and diseased vasculature. Tumor-homing peptides have been successfully used to target therapeutics and imaging agents to tumors. In vivo phage display has also been used in the identification of cell and/or tumor type-specific cell-penetrating peptides, which further facilitate the transmembrane delivery of various cargo molecules into cells. In this review we describe experimental setup for a combined ex vivo and in vivo screening procedure to select both conventional and cell-penetrating peptides homing to brain tumors.
authors.affiliation: Research Programs Unit, Translational Cancer Biology, Biomedicum Helsinki, University of Helsinki, Haartmaninkatu 8, 63, Helsinki, 00014, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Hyvönen
authors.forename: Pirjo
authors.initials: P
authors.lastname: Laakkonen
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D001932:Brain Neoplasms / Q000098:blood supply* / Q000188:drug therapy / Q000378:metabolism*; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000378:metabolism; D019151:Peptide Library
chemical_list: D057846:Cell-Penetrating Peptides; D019151:Peptide Library