title: [Effect of platelet-derived growth factor-BB on rat corpus cavernosum smooth muscle cell proliferation, migration and phenotypic modulation].
abstract: OBJECTIVE
  To study the effect of platelet-derived growth factor-BB (PDGFBBB) on rat corpus cavernosum smooth muscle (CCSM) cell proliferation, migration and phenotypic modulation and explore the underlying mechanisms.
  
  
  METHODS
  Wistar rat CCSM cells were obtained through a modified tissue culture method and identified by immunofluorescence assay. The effect of PDGFBB on the proliferation of CCSM cells was investigated using a CCK-8 kit and the optimum PDGFBB concentration for cell treatment was determined. CCSM cells were treated with vehicle or PDGF-BB at the optimum concentration, and the cell migration was examined using scratch assay; the mRNA expression of the transcription factor myocardin and the contractile phenotype markers αSMA and SMMHC in CCSM cells were determined by qRT-PCR at 24 h and 48 h. The protein expression of myocardin in CCSM cells incubated with PDGFBB for 0, 24 and 48 h was examined by Western blotting.
  
  
  RESULTS
  In CCSM cell culture, 96.5%and 96% of the cells were positive for αSMA and smoothelin, respectively. PDGFBB at different concentrations markedly promoted the proliferation of CCSM cells; the optimum PDGFBB concentration for enhancing cell proliferation was 12.5 ng/mL, which induced the migration of CCSM cells and significantly reduced the mRNA expressions of myocardin, αSMA and SMMHC (P<0.01). Exposure to PDGFBB decreased the protein expression of myocardin as the exposure time extended (within 48 h).
  
  
  CONCLUSIONS
  CCSM cells of a high purity can be obtained by the modified tissue culture method. PDGFBB can promote the proliferation and migration of CCSM cells and cause a phenotypic conversion from the contractile to the synthetic type possibly by down-regulating myocardin.
authors.affiliation: Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: medboy2012@163.com.
authors.forename: Fengzhi
authors.initials: F
authors.lastname: Chen
authors.forename: Shuhua
authors.initials: S
authors.lastname: He
authors.forename: Haitao
authors.initials: H
authors.lastname: Shan
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Lian
authors.forename: Anyang
authors.initials: A
authors.lastname: Wei
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000077214:Becaplermin; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D015536:Down-Regulation; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D018995:Myosin Heavy Chains / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010413:Penis / Q000166:cytology*; D010641:Phenotype; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D015534:Trans-Activators / Q000378:metabolism; D000099251:Myocardin
chemical_list: D000199:Actins; D000077214:Becaplermin; D018995:Myosin Heavy Chains; D009687:Nuclear Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D015534:Trans-Activators; D000099251:Myocardin; C000595077:Myh11 protein, rat; C498862:Acta2 protein, rat