title: Co-production of S-adenosyl-L-methionine and L-isoleucine in Corynebacterium glutamicum.
abstract: In this study, production of S-adenosyl-L-methionine in Corynebacterium glutamicum was investigated by overexpressing genes metK and vgb. Compared with vector control, overexpression of metK alone in C. glutamicum ATCC13032 and IWJ001 increased SAM production 5.11 and 11.65 times, respectively; while overexpression of metK and vgb in C. glutamicum ATCC13032 and IWJ001 increased SAM production 5.83 and 14.95 times, respectively. Further studies on IWJ001/pDXW-8-metk-vgb showed that the limiting factor for SAM production is intracellular ATP supply. Since IWJ001 is an L-isoleucine production strain, IWJ001/pDXW-8-metk-vgb could produce both SAM and L-isoleucine. After 72 h fermentation, SAM and L-isoleucine in IWJ001/pDXW-8-metk-vgb reached 0.67 g/L and 13.8 g/L, respectively. The results demonstrate the potential application of C. glutamicum for co-production of SAM and amino acids.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Han
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: xiaoyuanwang@hotmail.com.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial; D006420:Hemeproteins / Q000235:genetics / Q000378:metabolism; D007532:Isoleucine / Q000096:biosynthesis*; D008716:Methionine Adenosyltransferase / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D006420:Hemeproteins; D011994:Recombinant Proteins; C100771:Vgb protein, Bacteria; D007532:Isoleucine; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase
keywords: Corynebacterium glutamicum; L-isoleucine; L-methionine; Methionine adenosyltransferase; S-adenosyl-L-methionine