title: Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers.
abstract: BACKGROUND
  Heme oxygenase-1 (HO-1) protects cells by anti-oxidation, maintaining normal microcirculation and anti-inflammatory under stress. This study investigated the effects of biliary tract external drainage (BTED) on the expression levels of HO-1 in rat livers.
  
  
  METHODS
  Biliary tract external drainage was performed by inserting a cannula into the bile duct. Sixty Sprague-Dawley rats were randomized to the following groups: sham 1 h group; BTED 1 h group; bile duct ligation (BDL) 1 h group; sham 6 h group and BTED 6 h group. The expression levels of HO-1 mRNA were analyzed using real-time RT-PCR. The expression levels of HO-1 were analyzed using immunohistochemistry.
  
  
  RESULTS
  The expression levels of HO-1 mRNA in the liver of the BTED group increased significantly compared with the sham group 1 and 6 h after surgery (p < 0.05).The expression levels of HO-1 in the BTED group increased significantly compared with the sham group 1 and 6 h after surgery. The expression levels of HO-1 mRNA in the liver in the BDL group decreased significantly compared with the sham group 1 h after surgery (p < 0.05).The expression levels of HO-1 in the BDL group decreased significantly compared with the sham group at this time.
  
  
  CONCLUSIONS
  Biliary tract external drainages increase the expression levels of HO-1 in the liver.
authors.affiliation: Department of Emergency Intensive Care Unit, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. saibeiyin@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Emergency Intensive Care Unit, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. zhaobing124@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Emergency Intensive Care Unit, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. bichatlion@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Emergency Intensive Care Unit, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. malipostgraduate2@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Emergency Intensive Care Unit, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. chenerzhen@hotmail.com.
authors.forename: Er-Zhen
authors.initials: EZ
authors.lastname: Chen
authors.affiliation: Department of Emergency Intensive Care Unit, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. maoeq@yeah.net.
authors.forename: En-Qiang
authors.initials: EQ
authors.lastname: Mao
mesh_terms: D000818:Animals; D001659:Biliary Tract / Q000378:metabolism*; D001663:Bilirubin / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051547:Heme Oxygenase-1; D001663:Bilirubin