title: Role of TrkB during the postnatal development of the rat carotid body.
abstract: Brain-derived neurotrophic factor (BDNF) supports innervation of the carotid body by neurons projecting from the petrosal ganglion. Although carotid body glomus cells also express TrkB, BDNF's high affinity receptor, the role of BDNF in carotid body growth and O2 sensitivity has not been studied. Neonatal rats were treated with the TrkB antagonist K252a (100 μg kg(-1), i.p., b.i.d.) or vehicle on postnatal days P0-P6 and studied on P7. Carotid body volume was decreased by 35% after chronic K252a (P<0.001); a reduction in carotid body size was also elicited using the more selective TrkB antagonist ANA-12 (500 μg kg(-1), i.p., b.i.d.). In contrast, single-unit chemoafferent responses to 5% O2, measured in vitro, were unaffected by chronic K252a administration. Normoxic and hypoxic ventilation, measured by head-body plethysmography, were also normal after chronic K252a administration, but acute K252a administration produced a slower, deeper breathing pattern during the transition into hypoxia. These data suggest that BDNF regulates postnatal carotid body growth but does not influence the development of glomus cell O2 sensitivity.
authors.affiliation: Department of Biology, Bates College, 44 Campus Ave., Carnegie Science Hall, Lewiston, ME 04240, USA. Electronic address: rbavis@bates.edu.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: Bavis
authors.affiliation: Department of Biology, Bates College, 44 Campus Ave., Carnegie Science Hall, Lewiston, ME 04240, USA.
authors.forename: Halward J
authors.initials: HJ
authors.lastname: Blegen
authors.affiliation: Department of Biology, Bates College, 44 Campus Ave., Carnegie Science Hall, Lewiston, ME 04240, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Logan
authors.affiliation: Department of Biology, Bates College, 44 Campus Ave., Carnegie Science Hall, Lewiston, ME 04240, USA.
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Fallon
authors.affiliation: Department of Biology, Bates College, 44 Campus Ave., Carnegie Science Hall, Lewiston, ME 04240, USA.
authors.forename: Amy B
authors.initials: AB
authors.lastname: McDonough
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001381:Azepines / Q000494:pharmacology; D001549:Benzamides / Q000494:pharmacology; D002227:Carbazoles / Q000494:pharmacology; D002344:Carotid Body / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D026121:Indole Alkaloids / Q000494:pharmacology; D008297:Male; D009929:Organ Size; D010991:Plethysmography; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012119:Respiration / Q000187:drug effects
chemical_list: C559317:ANA 12 compound; D001381:Azepines; D001549:Benzamides; D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; C049985:staurosporine aglycone; D020813:Receptor, trkB
keywords: ANA-12; BDNF; Brain-derived neurotrophic factor; Control of breathing; Hypoxic ventilatory response; K252a