title: Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation.
abstract: Photo-cross-linking of tyramine-substituted hyaluronan (HA-Tyr) hydrogels is demonstrated for the first time. HA-Tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. Nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hMSCs) with high viability. Macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2D and 3D microfabrication. Different degrees of cross-linking induce different swelling/shrinking, allowing for light-induced microactuation. These new tools are complementary to the previously reported horseradish peroxidase/hydrogen peroxide cross-linking and allow sequential cross-linking of HA-Tyr matrices.
authors.affiliation: AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.|ETH Zurich , Cartilage Engineering + Regeneration, Department of Health Sciences and Technology, Otto-Stern-Weg 7, Zürich, 8093, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Loebel
authors.affiliation: ETH Zurich , Cartilage Engineering + Regeneration, Department of Health Sciences and Technology, Otto-Stern-Weg 7, Zürich, 8093, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Broguiere
authors.affiliation: AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.
authors.forename: Mauro
authors.initials: M
authors.lastname: Alini
authors.affiliation: ETH Zurich , Cartilage Engineering + Regeneration, Department of Health Sciences and Technology, Otto-Stern-Weg 7, Zürich, 8093, Switzerland.
authors.forename: Marcy
authors.initials: M
authors.lastname: Zenobi-Wong
authors.affiliation: AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Eglin
mesh_terms: D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D017785:Photons; D014439:Tyramine / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D006820:Hyaluronic Acid; D014439:Tyramine