title: The DnaJ OsDjA7/8 is essential for chloroplast development in rice (Oryza sativa).
abstract: DnaJ proteins belong to chaperones of Hsp40 family that ubiquitously participate in various cellular processes. Previous studies have shown chloroplast-targeted DnaJs are involved in the development of chloroplast in some plant species. However, little is known about the function of DnaJs in rice, one of the main staple crops. In this study, we characterized a type I DnaJ protein OsDjA7/8. We found that the gene OsDjA7/8 was expressed in all collected tissues, with a priority in the vigorous growth leaf. Subcellular localization revealed that the protein OsDjA7/8 was mainly distributed in chloroplast. Reduced expression of OsDjA7/8 in rice led to albino lethal at the seedling stage. Transmission electron microscopy observation showed that the chloroplast structures were abnormally developed in the plants silenced for OsDjA7/8. In addition, the transcriptional expression of the genes tightly associated with the development of chloroplast was deeply reduced in the plants silenced for OsDjA7/8. Collectively, our study reveals that OsDjA7/8 encodes a chloroplast-localized protein and is essential for chloroplast development and differentiation in rice.
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Sihui
authors.initials: S
authors.lastname: Liang
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Li
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Weilan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Bingtian
authors.initials: B
authors.lastname: Ma
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qin
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
authors.affiliation: Rice Research Institute, Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; State Key Laboratory of Hybrid Rice, Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China. Electronic address: xwchen88@163.com.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D036226:Seedlings / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D010940:Plant Proteins
keywords: Albino; Chloroplast development; DnaJ; OsDjA7/8; RNAi; Rice