title: Evaluation of immunochromatography tests for detection of novel GII.17 norovirus in stool samples.
abstract: A novel GII.17 norovirus has emerged as a major cause of epidemic and endemic acute gastroenteritis in several countries in Asia. We used a small panel of stool samples in which GII.17 virus had been quantified by real-time RT-PCR to evaluate four commercially available norovirus immunochromatography (IC) kits. At least 10(8) copies/mL of GII.17 virus were required by each IC kit for a positive result, which is 1,000-fold more than that reported for these assays for GII.4 viruses.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Khamrin
authors.forename: A
authors.initials: A
authors.lastname: Thongprachum
authors.forename: S
authors.initials: S
authors.lastname: Takanashi
authors.forename: S
authors.initials: S
authors.lastname: Okitsu
authors.forename: N
authors.initials: N
authors.lastname: Maneekarn
authors.forename: S
authors.initials: S
authors.lastname: Hayakawa
authors.forename: H
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000293:Adolescent; D000328:Adult; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D011933:Reagent Kits, Diagnostic