title: Paper diagnostic device for quantitative electrochemical detection of ricin at picomolar levels.
abstract: We report a paper-based assay platform for detection of ricin a chain. The paper platform is assembled by simple origami paper folding. The sensor is based on quantitative, electrochemical detection of silver nanoparticle labels linked to a magnetic microbead support via a ricin immunosandwich. Importantly, ricin was detected at concentrations as low as 34 pM. Additionally, the assay is robust, even in the presence of 100-fold excess hoax materials. Finally, the device is easily remediated after use by incineration. The cost of the device, not including reagents, is just $0.30. The total assay time, including formation of the immunosandwich, is 9.5 min.
authors.affiliation: Department of Chemistry, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712 USA. crooks@cm.utexas.edu.
authors.forename: Josephine C
authors.initials: JC
authors.lastname: Cunningham
authors.forename: Karen
authors.initials: K
authors.lastname: Scida
authors.forename: Molly R
authors.initials: MR
authors.lastname: Kogan
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.forename: Richard M
authors.initials: RM
authors.lastname: Crooks
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010209:Paper; D012276:Ricin / Q000032:analysis*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D012276:Ricin