title: [Synthesis and Identification of Artificial Antigen of Mangiferin].
abstract: OBJECTIVE
  To establish an enzyme linked immunosorbent assay(ELISA) for mangiferin (MRn), artificial antigen of MRn was synthesized.
  
  
  METHODS
  Oxidation method using sodium iodide was used to synthesize immunogenic antigen (MRn-BSA) and coating antigen(MRn-OVA) of MRn. The characterization of the synthesis was examined by UV spectrometry. BALB/c mice were immunized with the prepared MRn-BSA immunogenic antigen. The titer of the anti-serum was detected by ELISA, in the meanwhile the immunogenicity of MRn-BSA was confirmed by indirect competitive ELISA (icELISA).
  
  
  RESULTS
  UV spectroscopy showed that MRn was successfully conjugated with OVA and BSA,so the new compound of MRn-BSA was synthesized, the coupling ratio was 6: 1. After immuned MRn-BSA, the mice could produce anti-MRn antibodies specifically, of which titer was up to 1: 4 000, and the general range was 1 - 100 µg/mL.
  
  
  CONCLUSIONS
  Anti-MRn antibodies are discovered in the serum of mice, which indicates that artificial antigen of MRn is successfully synthesized,for the purpose of preparing monoclonal antibodies, as well as the establishment of appropriate immune methods.
authors.forename: Wen-ting
authors.initials: WT
authors.lastname: Hu
authors.forename: Hui-hua
authors.initials: HH
authors.lastname: Qu
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Hong
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Ren
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Liu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D044004:Xanthones / Q000276:immunology*
chemical_list: D000906:Antibodies; D000941:Antigens; D044004:Xanthones; C013592:mangiferin