title: Comparative proteomics analysis provide novel insight into laminitis in Chinese Holstein cows.
abstract: BACKGROUND
  Laminitis is considered as the most important cause of hoof lameness in dairy cows, which causes abundant economic losses in husbandry. Through intense efforts in past decades, the etiology of laminitis is preliminarily considered to be subacute ruminal acidosis; however, the pathogenesis of laminitis needs further research. The differentially expressed proteins (DEP) were detected in plasma of healthy cows and clinical laminitis cows by two-dimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
  
  
  RESULTS
  Nineteen protein spots were differentially expressed, and 16 kinds of proteins were identified after peptide mass fingerprint search and bioinformatics analysis. Of these, 12 proteins were differentially up-regulated and 4 down-regulated. Overall, these differential proteins were involved in carbohydrate metabolism, lipids metabolism, molecular transport, immune regulation, inflammatory response, oxidative stress and so on.
  
  
  CONCLUSIONS
  The DEPs were closely related to the occurrence and development of laminitis and the lipid metabolic disturbance may be a new pathway to cause laminitis in dairy cows. The results provide the theory foundation for further revealing the mechanism of laminitis and screening the early diagnostic proteins and therapeutic target.
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. dongshuwei@caas.cn.|Key Laboratory of Yak Breeding Engineering, Lanzhou, 730050, People's Republic of China. dongshuwei@caas.cn.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Dong
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. zhangshidong@caas.cn.
authors.forename: Shi-Dong
authors.initials: SD
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. wangdongsheng@caas.cn.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Wang
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. wanghui01@caas.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. shangxf928@163.com.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Shang
authors.affiliation: Key Laboratory of Yak Breeding Engineering, Lanzhou, 730050, People's Republic of China. yanping@caas.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Yan
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. yanping@caas.cn.
authors.forename: Zuo-Ting
authors.initials: ZT
authors.lastname: Yan
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture/ Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China. yanzuoting@caas.cn.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D002681:China; D005534:Foot Diseases / Q000378:metabolism / Q000662:veterinary*; D005786:Gene Expression Regulation; D006724:Hoof and Claw / Q000473:pathology*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation