title: DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity.
abstract: The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated. Based on this unique quenching effect and the DNA-mediated ScGFP/GO interaction, a label-free fluorescence method has been established for homogeneously assaying the activity and inhibition of base excision repair enzyme.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. huangyan.hnu@gmail.com niezhou.hnu@gmail.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Lijun
authors.initials: L
authors.lastname: Li
authors.forename: Daiqi
authors.initials: D
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D010087:Oxides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D051981:Uracil-DNA Glycosidase / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D049452:Green Fluorescent Proteins; D006108:Graphite; D004247:DNA; D051981:Uracil-DNA Glycosidase; D045643:DNA Repair Enzymes