title: Live births following Karyomapping of human blastocysts: experience from clinical application of the method.
abstract: The clinical application of a new, widely applicable method known as Karyomapping to carry out a total of 55 clinical cases of preimplantation genetic diagnosis (PGD) for single gene disorders is reported. Conventional polymerase chain reaction (PCR) testing was carried out in parallel to the new method for all cases. Clinical application of Karyomapping in this study resulted in three live births and nine clinical pregnancies out of 20 cases with a transfer. All in all, results presented in this study indicate that Karyomapping is a highly efficient, accurate and robust method for PGD of single gene disorders. Karyomapping can offer a more comprehensive assessment of the region of interest than conventional PCR analysis, allowing for more embryos to receive diagnosis (99.6% versus 96.8%), whereas its wide applicability reduces substantially the time that patients have to wait before starting their in vitro fertilization (IVF) cycle. Nonetheless, inclusion of elements of conventional PCR methodology, such as direct mutation detection, may be required in cases in which the gene of interest is in a region with reduced single nucleotide polymorphism (SNP) coverage (e.g. telomeric regions), when offering PGD for consanguineous couples, or in cases where no samples from additional family members are available.
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA. Electronic address: mkonstantinidis@reprogenetics.com.
authors.forename: Michalis
authors.initials: M
authors.lastname: Konstantinidis
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Renata
authors.initials: R
authors.lastname: Prates
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: N-Neka
authors.initials: NN
authors.lastname: Goodall
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Jill
authors.initials: J
authors.lastname: Fischer
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Tecson
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Tsion
authors.initials: T
authors.lastname: Lemma
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Chu
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Jordan
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Erin
authors.initials: E
authors.lastname: Armenti
authors.affiliation: Reprogenetics UK, Institute of Reproductive Sciences, Oxford Business Park North, Oxford, OX4 2HW, UK; Nuffield Department of Obstetrics and Gynaecology, Women's Centre, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, UK.
authors.forename: Dagan
authors.initials: D
authors.lastname: Wells
authors.affiliation: Reprogenetics LLC, 3 Regent Street, Livingston, NJ 07039, USA.
authors.forename: Santiago
authors.initials: S
authors.lastname: Munné
mesh_terms: D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D006801:Humans; D059785:Karyotype; D007621:Karyotyping; D019836:Preimplantation Diagnosis / Q000379:methods*
keywords: Karyomapping; gene disorders; preimplantation genetic diagnosis; single nucleotide polymorphism arrays