title: Gender-dependent and genotype-sensitive monoaminergic changes induced by polychlorinated biphenyl 153 in the rat brain.
abstract: Polychlorinated biphenyls (PCBs) are present as ortho- and non-ortho-substituted PCBs, with most of the ortho-substituted congeners being neurotoxic. The present study examined effects of the ortho-substituted PCB 153 on dopamine, serotonin and amino acid neurotransmitters in the neostriatum of both male and female Wistar Kyoto (WKY) and spontaneously hypertensive rat (SHR) genotypes. PCB 153 exposure at p8, p14 and p20 had no effects on levels of these transmitters when examined at p55, but led to increased levels of both homovanillic acid and 5-hydroxyindoleacetic acid, the degradation products of dopamine and serotonin, respectively, in all groups except the female SHR. Immunoblotting showed that PCB exposure induced gender-specific decreases in dopaminergic synaptic proteins. These included a novel finding of decreased levels of the dopamine D5 receptor in both genders and genotypes, whereas male-specific changes included decreases in the postsynaptic density (PSD)-95 protein in the WKY and SHRs and a decrease in the presynaptic dopamine transporter in both the WKY and, less clearly in the male SHR. A female-specific tendency of increased vesicular monoamine transporter-2 was observed in the SHRs after PCB exposure. No changes were seen in tyrosine hydroxylase, the cytoskeletal neurotubulin or the plasma membrane marker Na(+)/K(+)-ATPase in any strain. Hence, PCB-exposure led to increases in monoamine transmitter turnover in both male and female animals, whereas decreases in both pre- and postsynaptic dopaminergic proteins were predominantly seen in male animals. PCB 153 may therefore induce neostriatal toxicity through both presynaptic and postsynaptic mechanisms in both genotypes and genders, including effects on the aspiny interneurons, which employ the D5 receptor to mediate dopamine effects on interneurons in the basal ganglia.
authors.affiliation: Department of Biochemistry, Division of Molecular Medicine, Institute of Basic Medical Science, University of Oslo, Norway.
authors.forename: Kine S N
authors.initials: KS
authors.lastname: Dervola
authors.affiliation: Oslo and Akershus University College of Applied Sciences, Oslo, Norway.
authors.forename: Espen B
authors.initials: EB
authors.lastname: Johansen
authors.affiliation: Department of Biochemistry, Division of Molecular Medicine, Institute of Basic Medical Science, University of Oslo, Norway. Electronic address: s.i.walaas@medisin.uio.no.
authors.forename: S Ivar
authors.initials: SI
authors.lastname: Walaas
authors.affiliation: Department of Biochemistry, Division of Molecular Medicine, Institute of Basic Medical Science, University of Oslo, Norway.
authors.forename: Frode
authors.initials: F
authors.lastname: Fonnum
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D055664:Electrochemical Techniques; D004785:Environmental Pollutants / Q000494:pharmacology*; D005260:Female; D005838:Genotype; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D050641:Receptors, Dopamine D5 / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D012727:Sex Characteristics
chemical_list: D015306:Biogenic Monoamines; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D050483:Dopamine Plasma Membrane Transport Proteins; D004785:Environmental Pollutants; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D018377:Neurotransmitter Agents; D050641:Receptors, Dopamine D5; D012701:Serotonin; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl
keywords: Dopamine; Gender; Genotype; Metabolism; Polychlorinated biphenyls; Serotonin