title: Molecular mapping of five soybean genes involved in male-sterility, female-sterility.
abstract: In soybean, asynaptic and desynaptic mutants lead to abnormal meiosis and fertility reduction. Several male-sterile, female-sterile mutants have been identified and studied in soybean, however, some of these mutants have not been mapped to locations on soybean chromosomes. The objectives of this study were to molecularly map five male-sterile, female-sterile genes (st2, st4, st5, st6, and st7) in soybean and compare the map locations of these genes with already mapped sterility genes. Microsatellite markers were used in bulked segregant analyses to locate all five male-sterile, female-sterile genes to soybean chromosomes, and markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The st2, st4, st5, st6, and st7 genes were located on molecular linkage group (MLG) B1 (chromosome 11), MLG D1a (chromosome 01), MLG F (chromosome 13), MLG B2 (chromosome 14), and D1b (chromosome 02), respectively. The st2, st4, st5, st6, and st7 genes were flanked to 10.3 (∼ 399 kb), 6.3 (∼ 164 kb), 3.9 (∼ 11.8 Mb), 11.0 (∼ 409 kb), and 5.3 cM (∼ 224 kb), and the flanked regions contained 57, 17, 362, 52, and 17 predicted genes, respectively. Future characterization of candidate genes should facilitate identification of the male- and female-fertility genes, which may provide vital insights on structure and function of genes involved in the reproductive pathway in soybean.
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Speth
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Rogers
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Napatsakorn
authors.initials: N
authors.lastname: Boonyoo
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: VanMeter
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Baumbach
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Alina
authors.initials: A
authors.lastname: Ott
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Jerott
authors.initials: J
authors.lastname: Moore
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: Cina
authors.affiliation: b Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA.
authors.forename: Reid
authors.initials: R
authors.lastname: Palmer
authors.affiliation: a Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, WI 54481, USA.
authors.forename: Devinder
authors.initials: D
authors.lastname: Sandhu
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D009154:Mutation; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*
chemical_list: D010940:Plant Proteins
keywords: Glycine max; carte génétique; genetic linkage map; male-sterile female-sterile; simple sequence repeat; sterility; stérilité; stérilité mâle et femelle; séquence simple répétée