title: Erufosine, a phosphoinositide-3-kinase inhibitor, to mitigate posterior capsule opacification in the human capsular bag model.
abstract: OBJECTIVE
  To determine whether erufosine alone or erufosine-loaded intraocular lenses (IOLs) can inhibit growth of human lens epithelial cells after a single administration in the human capsular bag model.
  
  
  METHODS
  Laboratory for Cell Biology, Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Sixteen human cadaver eyes had sham cataract surgery. The capsular bag was transferred into cell culture. The tissue was exposed to the half maximum inhibitory concentrations of erufosine alone for 72 hours; solvent-only tissue served as a control. Erufosine is a potent inhibitor of phosphoinositide-3-kinase, a downstream kinase with major implications in posterior capsule opacification (PCO) pathogenesis. The IOLs were soaked with erufosine and implanted in the capsular bags; unsoaked IOLs served as controls. For both settings, the time until confluence of the capsular bag was measured. Cell growth was observed and photodocumented.
  
  
  RESULTS
  Erufosine as a single therapeutic agent increased the time until confluence of the capsular bag, but not significantly compared with the control. When IOLs were soaked with erufosine, a long-term prophylactic effect was observed in this organ model for PCO, which is known to closely reflect the clinical situation.
  
  
  CONCLUSIONS
  Erufosine-soaked IOLs effectively inhibited PCO formation as seen in long-term organ culture and might become of clinical relevance.
  
  
  BACKGROUND
  Drs. Kampik and Eibl-Lindner are inventors of IOLs treated with alkylphosphocholines for pharmacological after-cataract prophylaxis, patent international application PCT/EP2010/051490. No other author has a financial or proprietary interest in any material or method mentioned.
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. Electronic address: christian.wertheimer@med.uni-muenchen.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Wertheimer
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Brandlhuber
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kook
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Mayer
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Laubichler
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Armin
authors.initials: A
authors.lastname: Wolf
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Anselm
authors.initials: A
authors.lastname: Kampik
authors.affiliation: From the Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Eibl-Lindner
mesh_terms: D000328:Adult; D000368:Aged; D058442:Capsule Opacification / Q000517:prevention & control*; D004337:Drug Carriers; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D007903:Lens Capsule, Crystalline; D019654:Lens Implantation, Intraocular; D007910:Lenses, Intraocular; D008875:Middle Aged; D008954:Models, Biological; D009924:Organ Culture Techniques; D010755:Organophosphates / Q000494:pharmacology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D057852:Posterior Capsule of the Lens / Q000187:drug effects*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D014019:Tissue Donors
chemical_list: D004337:Drug Carriers; D004791:Enzyme Inhibitors; D010755:Organophosphates; D000081082:Phosphoinositide-3 Kinase Inhibitors; D000644:Quaternary Ammonium Compounds; C472787:erucylphospho-N,N,N-trimethylpropylammonium