title: Multi-response optimization of factors affecting ultrasonic assisted extraction from Iranian basil using central composite design.
abstract: The present study reports on the extraction of antioxidant compounds from Iranian Ocimum basilicum. Central composite design (CCD) was used to investigate the effect of extraction variables on the ultrasound-assisted extraction (UAE). Three independent variables including temperature, methanol to water ratio percent, and sonication time were studied for simultaneous optimization of antioxidant capacity, total phenolic content and extraction yield. Both quantitative modeling and response surface methodology suggested that methanol to water ratio percent and extraction temperature were the most effective parameters of UAE process. However, sonication time was found out to be an insignificant factor in ultrasound-assisted extraction of antioxidant and total phenolic compounds of O. basilicum. The optimum conditions were determined as temperature of 59 °C, methanol to water ratio of 65.2% (v/v), and extraction time of 20 min.
authors.affiliation: Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Izadiyan
authors.affiliation: Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Chemistry, Shiraz University, Shiraz, Iran. Electronic address: hemmatb@shirazu.ac.ir.
authors.forename: Bahram
authors.initials: B
authors.lastname: Hemmateenejad
mesh_terms: D000975:Antioxidants; D007492:Iran; D018649:Ocimum basilicum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts
keywords: Antioxidant capacity; Central composite design; Ocimum basilicum; Ultrasound-assisted extraction