title: Colorimetric and fluorescence quenching aptasensors for detection of streptomycin in blood serum and milk based on double-stranded DNA and gold nanoparticles.
abstract: Antibiotic residues in animal foodstuffs are of great concern to consumers. In this study, fluorescence quenching and colorimetric aptasensors were designed for detection of streptomycin based on aqueous gold nanoparticles (AuNPs) and double-stranded DNA (dsDNA). In the absence of streptomycin, aptamer/FAM-labeled complementary strand dsDNA is stable, resulting in the aggregation of AuNPs by salt and an obvious color change from red to blue and strong emission of fluorescence. In the presence of streptomycin, aptamer binds to its target and FAM-labeled complementary strand adsorbs on the surface of AuNPs. So the well-dispersed AuNPs remain stable against salt-induced aggregation with a wine-red color and the fluorescence of FAM-labeled complimentary strand is efficiently quenched by AuNPs. The colorimetric and fluorescence quenching aptasensors showed excellent selectivity toward streptomycin with limit of detections as low as 73.1 and 47.6 nM, respectively. The presented aptasensors were successfully used to detect streptomycin in milk and serum.
authors.affiliation: Cardiovascular Research Center, Ghaem hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ahmad Sarreshtehdar
authors.initials: AS
authors.lastname: Emrani
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; Research Institute of Sciences and New Technology, Mashhad, Iran.
authors.forename: Noor Mohammad
authors.initials: NM
authors.lastname: Danesh
authors.affiliation: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Parirokh
authors.initials: P
authors.lastname: Lavaee
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ramezani
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir.
authors.forename: Khalil
authors.initials: K
authors.lastname: Abnous
authors.affiliation: Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Taghdisi
mesh_terms: D000818:Animals; D052158:Aptamers, Peptide / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry*; D013307:Streptomycin / Q000737:chemistry*
chemical_list: D052158:Aptamers, Peptide; D006046:Gold; D004247:DNA; D013307:Streptomycin
keywords: Aptamer; Colorimetric; Fluorescence; Sensor; Streptomycin