title: Combined effect of hydrogen sulphide donor and losartan in experimental diabetic nephropathy in rats.
abstract: BACKGROUND
  Diabetic nephropathy (DN) is one of the complex complications of Diabetes Mellitus (DM). The present study has been designed to examine protective role of hydrogen Sulphide (H2S) donor against streptozotocin (STZ) -induced behavioral, oxidative abnormalities and its DN like symptoms in rats.
  
  
  METHODS
  For the induction of DN single intraperitoneal administration of STZ (45 mg/kg) was given till third week. Behavioral parameters were measured on 1st, 7th, 21st and 42nd days and biochemical parameters were performed on 42nd day. All the drug treatments [NaHS (10 & 30 μmol/kg i.p), DL-propargylglycine (10 mg/kg i.p), standard drug- Losartan (5 mg/kg p.o)] were given for 3 weeks staring from 21st day after the STZ injection.
  
  
  RESULTS
  Three weeks treatment with sodium hydrosulphide (NaHS) (10 and 30 μmol/kg i.p,) significantly attenuated the behavioral and biochemical abnormalities in STZ-treated animals. DL-propargylglycine (10 mg/kg i.p) pretreatment with sub-effective dose of NaHS (30 μmol/kg i.p) significantly reversed the protective effect of NaHS. However, combination of both NaHS (30 μmol/kg i.p) and standard drug losartan (5 mg/kg p.o) potentiated their effects as compared to their effect alone.
  
  
  CONCLUSIONS
  The results of the present study suggest that H2S treatment showed significant improvement in behavioral and biochemical abnormalities induced by STZ administration. Thus H2S represents a target of treatment to prevent the progression of complications by DN.
authors.affiliation: Pharmacology Division, Department of Pharmacology, ISF College of Pharmacy, Moga, 14200 Punjab India.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Kaur
authors.affiliation: Pharmacology Division, Department of Pharmacology, ISF College of Pharmacy, Moga, 14200 Punjab India.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Sachdeva
authors.affiliation: Pharmacology Division, Department of Pharmacology, ISF College of Pharmacy, Moga, 14200 Punjab India.
authors.forename: Onkar
authors.initials: O
authors.lastname: Bedi
authors.affiliation: Pharmacology Division, Department of Pharmacology, ISF College of Pharmacy, Moga, 14200 Punjab India.
authors.forename: Tavleen
authors.initials: T
authors.lastname: Kaur
authors.affiliation: Pharmacology Division, Department of Pharmacology, ISF College of Pharmacy, Moga, 14200 Punjab India.
authors.forename: Puneet
authors.initials: P
authors.lastname: Kumar
keywords: Diabetes mellitus; Diabetic nephropathy; Sodium hydrosulphide; Streptozotocin