title: Some properties and possible biological role of peptidase inhibitors from the entomopathogenic fungus Tolypocladium cylindrosporum.
abstract: The activities of secreted and mycelial inhibitors of proteolytic enzymes from fungi of the order Hypocreales have been investigated. Inhibitors of bromelain, papain, and trypsin of low molecular mass (about 1 kDa) and a subtilisin proteinaceous inhibitor with molecular mass of 45 kDa were revealed in the culture liquid of the fungus Tolypocladium cylindrosporum. The subtilisin inhibitor from T. cylindrosporum has antibiotic properties, significantly decreased the activity of purified bacterial enzymes, and prevented the growth of the bacterium Pseudomonas sp. Data suggesting the existence in fungi of the Hypocreales order of two pools of peptidase inhibitors have been obtained.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Popova
authors.forename: Y E
authors.initials: YE
authors.lastname: Dunaevsky
authors.forename: V I
authors.initials: VI
authors.lastname: Domash
authors.forename: T A
authors.initials: TA
authors.lastname: Semenova
authors.forename: G A
authors.initials: GA
authors.lastname: Beliakova
authors.forename: M A
authors.initials: MA
authors.lastname: Belozersky
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004798:Enzymes / Q000378:metabolism; D006999:Hypocreales / Q000737:chemistry*; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004798:Enzymes; D011480:Protease Inhibitors