title: Glycation of bovine serum albumin by ascorbate in vitro: Possible contribution of the ascorbyl radical?
abstract: Ascorbic acid (AA) has been reported to be both pro-and antiglycating agent. In vitro, mainly proglycating effects of AA have been observed. We studied the glycation of bovine serum albumin (BSA) induced by AA in vitro. BSA glycation was accompanied by oxidative modifications, in agreement with the idea of glycoxidation. Glycation was inhibited by antioxidants including polyphenols and accelerated by 2,​2'-​azobis-​2-​methyl-​propanimidamide and superoxide dismutase. Nitroxides, known to oxidize AA, did not inhibit BSA glycation. A good correlation was observed between the steady-state level of the ascorbyl radical in BSA samples incubated with AA and additives and the extent of glycation. On this basis we propose that ascorbyl radical, in addition to further products of AA oxidation, may initiate protein glycation.
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland. Electronic address: isadowska@poczta.fm.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sadowska-Bartosz
authors.affiliation: Teaching and Research Center of Microelectronics and Nanotechnology, Faculty of Mathematics and Natural Sciences, University of Rzeszów, Poland.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Stefaniuk
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Galiniak
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland; Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000578:Amidines / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001205:Ascorbic Acid / Q000737:chemistry*; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006031:Glycosylation; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D059808:Polyphenols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013482:Superoxide Dismutase / Q000737:chemistry
chemical_list: D000578:Amidines; D000975:Antioxidants; D002614:Chelating Agents; D005609:Free Radicals; D017127:Glycation End Products, Advanced; D016877:Oxidants; D059808:Polyphenols; D012996:Solutions; D012710:Serum Albumin, Bovine; D013482:Superoxide Dismutase; D001205:Ascorbic Acid
keywords: Antioxidant; Ascorbic acid; Ascorbyl free radical; Bovine serum albumin; Glycation