title: The effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast's of acute lymphoblastic leukemia patients.
abstract: BACKGROUND
  Chemotherapy plays a very important role in the treatment of leukemia but the resistance properties of the lymphoblasts limit the effect of chemotherapy. One of the main mechanisms of resistance to chemotherapy is the increased expression of MDR1 gene. The aim of this study was to explore the effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast of new cases of acute lymphoblastic leukemia (ALL) patients in vitro.
  
  
  METHODS
  Separation of lymphoblasts of 5 new case ALL patients from peripheral blood was performed by ficoll density gradient centrifugation. Lymphoblasts were cultured in RPMI 1640 medium. Lymphoblasts were treated with 50µmol/L resveratrol for 48 h. Total RNA was extracted with guanidine isothiocyanate. RNA was converted to cDNA. Real time PCR was used to detect mRNA expression of MDR1.
  
  
  RESULTS
  The results of gene detection showed that the expression of MDR1 did not change significantly in the patients however, in one patient expression of MDR1 increased upon treatment with resveratrol.
  
  
  CONCLUSIONS
  The results of this study did not support resveratrol as a compound to reverse multidrug resistance in leukemic lymphoblasts.
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Hosein Poor Feyzi
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Farshdousti Hagh
authors.affiliation: Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Tohid
authors.initials: T
authors.lastname: Ebadi
authors.affiliation: Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Karim
authors.initials: K
authors.lastname: Shams Asanjan
authors.affiliation: Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Movasagpoor Akbari
authors.affiliation: Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Talebi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Iran University of Medical Science, Tehran, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Emadi
keywords: ALL; Leukemic lymphoblast; Multidrug resistance gene; Resveratrol