title: MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL.
abstract: Nocardia is a ubiquitous microorganism related to pyogranulomatous infection, which is difficult to treat in humans and animals. The occurrence of the disease is on the rise in many countries due to an increase in immunosuppressive diseases and treatments. This report of cases from Brazil presents the genotypic characterization and the antimicrobial susceptibility pattern using the disk-diffusion method and inhibitory minimal concentration with E-test® strips. In summary, this report focuses on infections in young adult men, of which three cases were cutaneous, two pulmonary, one neurological and one systemic. The pulmonary, neurological and systemic cases were attributed to immunosuppressive diseases or treatments. Sequencing analysis of the 16S rRNA segments (1491 bp) identified four isolates of Nocardia farcinica, two isolates of Nocardia nova and one isolate of Nocardia asiatica. N. farcinica was involved in two cutaneous, one systemic and other pulmonary cases; N. nova was involved in one neurological and one pulmonary case; and Nocardia asiatica in one cutaneous case. The disk-diffusion antimicrobial susceptibility test showed that the most effective antimicrobials were amikacin (100%), amoxicillin/clavulanate (100%), cephalexin (100%) and ceftiofur (100%), while isolates had presented most resistance to gentamicin (43%), sulfamethoxazole/trimethoprim (43%) and ampicillin (29%). However, on the inhibitory minimal concentration test (MIC test), only one of the four isolates of Nocardia farcinica was resistant to sulfamethoxazole/trimethoprim.
authors.affiliation: Department Veterinary Hygiene and Public Health, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Sao Paulo, Brazil.
authors.forename: Larissa Anuska Zeni
authors.initials: LA
authors.lastname: Condas
authors.affiliation: Department Veterinary Hygiene and Public Health, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Sao Paulo, Brazil.
authors.forename: Márcio Garcia
authors.initials: MG
authors.lastname: Ribeiro
authors.affiliation: Clinical Hospital, Universidade Federal do Parana, Curitiba, PR, Brazil.
authors.forename: Marisol Domingues
authors.initials: MD
authors.lastname: Muro
authors.affiliation: Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Agueda Palmira Castagna
authors.initials: AP
authors.lastname: de Vargas
authors.affiliation: Medical Mycology Research Centre, Chiba University, Chiba, Japan.
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Matsuzawa
authors.affiliation: Medical Mycology Research Centre, Chiba University, Chiba, Japan.
authors.forename: Katsukiyo
authors.initials: K
authors.lastname: Yazawa
authors.affiliation: Department Veterinary Hygiene and Public Health, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Sao Paulo, Brazil.
authors.forename: Amanda Keller
authors.initials: AK
authors.lastname: Siqueira
authors.affiliation: Department Veterinary Hygiene and Public Health, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Sao Paulo, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Salerno
authors.affiliation: Department Veterinary Hygiene and Public Health, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Sao Paulo, Brazil.
authors.forename: Gustavo Henrique Batista
authors.initials: GH
authors.lastname: Lara
authors.affiliation: Department Veterinary Hygiene and Public Health, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Sao Paulo, Brazil.
authors.forename: Rafaela Mastrangelo
authors.initials: RM
authors.lastname: Risseti
authors.affiliation: Department of Biological Science, Microbiology, Immunology and Parasitology Sector, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Karen Spadari
authors.initials: KS
authors.lastname: Ferreira
authors.affiliation: Medical Mycology Research Centre, Chiba University, Chiba, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Gonoi
mesh_terms: D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D052978:Disk Diffusion Antimicrobial Tests; D006801:Humans; D008297:Male; D009615:Nocardia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009617:Nocardia Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S