title: ATP4 and ciliation in the neuroectoderm and endoderm of Xenopus embryos and tadpoles.
abstract: During gastrulation and neurulation, foxj1 expression requires ATP4a-dependent Wnt/β-catenin signaling for ciliation of the gastrocoel roof plate (Walentek et al. Cell Rep. 1 (2012) 516-527.) and the mucociliary epidermis (Walentek et al. Dev. Biol. (2015)) of Xenopus laevis embryos. These data suggested that ATP4a and Wnt/β-catenin signaling regulate foxj1 throughout Xenopus development. Here we analyzed whether foxj1 expression was also ATP4a-dependent in other ciliated tissues of the developing Xenopus embryo and tadpole. We found that in the floor plate of the neural tube ATP4a-dependent canonical Wnt signaling was required for foxj1 expression, downstream of or in parallel to Hedgehog signaling. In the developing tadpole brain, ATP4-function was a prerequisite for the establishment of cerebrospinal fluid flow. Furthermore, we describe foxj1 expression and the presence of multiciliated cells in the developing tadpole gastrointestinal tract. Our work argues for a general requirement of ATP4-dependent Wnt/β-catenin signaling for foxj1 expression and motile ciliogenesis throughout Xenopus development.
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany ; Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California at Berkeley, Berkeley, California 94720, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Walentek
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Hagenlocher
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Beyer
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Müller
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Feistel
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Schweickert
authors.affiliation: Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California at Berkeley, Berkeley, California 94720, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Harland
authors.affiliation: Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Blum
keywords: ATP4a; Cilia; Gastric H+/K+ATPase; Wnt signaling; Xenopus