title: Tyrosinase conjugated reduced graphene oxide based biointerface for bisphenol A sensor.
abstract: We have fabricated a nanocomposite of reduced graphene oxide (rGO) sheets and chitosan (Cn) polymer based highly sensitive electrochemical biosensor for detection of bisphenol A (BPA). The two-dimensional structure and chemical functionality of rGO and Cn provide an excellent electrode surface for loading of tyrosinase enzyme molecules. This rGO-Cn nanocomposite is capable of effectively utilizing their superior conductivity, larger effective surface area and superior electrochemical performance due to its synergistic effect between rGO and Cn. The structural, morphological and electrochemical characterizations of nanocomposite sheets have been performed by electron microscopy, X-ray diffraction, FTIR and Potentiostat/Galvanostat techniques. This fabricated biosensor is sensitive to nanomolar (0.74 nM) concentration of BPA and detection time is 10s compared to conventional BPA ELISA kit (0.3 µg/L and 2.5h). The rGO-Cn based biosensor exhibits a higher sensitivity (83.3 µA nM(-1) cm(-2)), wider linearity (0.01-50 µM) with good selectivity towards BPA. This biosensor is capable to quantify real sample of BPA using packaged drinking water bottles. This rGO-Cn nanocomposite sheets emerges as a potential electrode material for detection of other estrogenic substrate.
authors.affiliation: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India. Electronic address: kamilreza@gmail.com.
authors.forename: K Kamil
authors.initials: KK
authors.lastname: Reza
authors.affiliation: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Md Azahar
authors.initials: MA
authors.lastname: Ali
authors.affiliation: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Ved Varun
authors.initials: VV
authors.lastname: Agrawal
authors.affiliation: Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors.forename: A M
authors.initials: AM
authors.lastname: Biradar
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D048271:Chitosan; D004800:Enzymes, Immobilized; D006108:Graphite; D014442:Monophenol Monooxygenase; D058726:Nanoconjugates; D010087:Oxides; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Electrochemical sensor; Nanocomposite; Reduced graphene oxide; Tyrosinase