title: Bioorthogonal SERS Nanoprobes for Mulitplex Spectroscopic Detection, Tumor Cell Targeting, and Tissue Imaging.
abstract: A surface-enhanced Raman scattering (SERS) technique shows extraordinary features for a range of biological and biomedical applications. Herein, a series of novel bioorthogonal SERS nanoprobes were constructed with Gold nanoflower (AuNF) and Raman reporters, the signals of which were located in a Raman-silent region of biological samples. AS1411 aptamer was also co-conjugated with AuNF through a self-assembled monolayer coverage strategy. Multiplex SERS imaging using these nanoprobes with three different bioorthogonal small-molecule Raman reporters is successfully achieved with high multiplexing capacity in a biologically Raman-silent region. These Raman nanoprobes co-conjugated with AS1411 showed high affinity for tumor cells with overexpressed nucleolin and can be used for selective tumor cell screening and tissue imaging.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, No. 38, Xueyuan Rd. Beijing 100191 (P.R. China).
authors.forename: Junzhou
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, No. 38, Xueyuan Rd. Beijing 100191 (P.R. China).
authors.forename: Duanwei
authors.initials: D
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, No. 38, Xueyuan Rd. Beijing 100191 (P.R. China).
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Technical Institute of Physics and Chemistry the Chinese Academy of Sciences.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Technical Institute of Physics and Chemistry the Chinese Academy of Sciences.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Technical Institute of Physics and Chemistry the Chinese Academy of Sciences.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, No. 38, Xueyuan Rd. Beijing 100191 (P.R. China). xinjingt@bjmu.edu.cn.
authors.forename: Xinjing
authors.initials: X
authors.lastname: Tang
mesh_terms: D001686:Biological Phenomena; D045744:Cell Line, Tumor / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D006046:Gold
keywords: AS1411; Raman spectroscopy; aptamers; bioorthogonality; cell targeting; nanoprobes