title: Tissue distribution of pretomanid in rat brain via mass spectrometry imaging.
abstract: 1. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological analyses of the distribution of drugs in tissue. Pretomanid is a pro-drug belonging to a class of antibiotics known as nitroimidizoles, which have been proven to be active under hypoxic conditions and to the best of our knowledge there have been no studies investigating the distribution and localisation of this class of compounds in the brain using MALDI MSI. 2. Herein, we report on the distribution of pretomanid in the healthy rat brain after intraperitoneal administration (20 mg/kg) using MALDI MSI. Our findings showed that the drug localises in specific compartments of the rat brain viz. the corpus callosum, a dense network of neurons connecting left and right cerebral hemispheres. 3. This study proves that MALDI MSI technique has great potential for mapping the pretomanid distribution in uninfected tissue samples, without the need for molecular labelling.
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Adeola
authors.initials: A
authors.lastname: Shobo
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Dominika
authors.initials: D
authors.lastname: Bratkowska
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Sooraj
authors.initials: S
authors.lastname: Baijnath
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Suhashni
authors.initials: S
authors.lastname: Naiker
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Anou M
authors.initials: AM
authors.lastname: Somboro
authors.affiliation: b Biomedical Resource Unit , University of KwaZulu-Natal , Durban , South Africa.
authors.forename: Linda A
authors.initials: LA
authors.lastname: Bester
authors.affiliation: b Biomedical Resource Unit , University of KwaZulu-Natal , Durban , South Africa.
authors.forename: Sanil D
authors.initials: SD
authors.lastname: Singh
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Tricia
authors.initials: T
authors.lastname: Naicker
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Hendrik G
authors.initials: HG
authors.lastname: Kruger
authors.affiliation: a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.
authors.forename: Thavendran
authors.initials: T
authors.lastname: Govender
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000493:pharmacokinetics*; D001812:Blood-Brain Barrier / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D005260:Female; D009593:Nitroimidazoles / Q000493:pharmacokinetics*; D011355:Prodrugs / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014018:Tissue Distribution
chemical_list: D000995:Antitubercular Agents; D009593:Nitroimidazoles; D011355:Prodrugs; C410767:pretomanid
keywords: Drug distribution; MALDI MSI; nitroimidazole