title: A multimodal nano agent for image-guided cancer surgery.
abstract: Intraoperative imaging technologies including computed tomography and fluorescence optical imaging are becoming routine tools in the cancer surgery operating room. They constitute an enabling platform for high performance surgical resections that assure local control while minimizing morbidity. New contrast agents that can increase the sensitivity and visualization power of existing intraoperative imaging techniques will further enhance their clinical benefit. We report here the development, detection and visualization of a dual-modality computed tomography and near-infrared fluorescence nano liposomal agent (CF800) in multiple preclinical animal models of cancer. We describe the successful application of this agent for combined preoperative computed tomography based three-dimensional surgical planning and intraoperative target mapping (>200 Hounsfield Units enhancement), as well as near-infrared fluorescence guided resection (>5-fold tumor-to-background ratio). These results strongly support the clinical advancement of this agent for image-guided surgery with potential to improve lesion localization, margin delineation and metastatic lymph node detection.
authors.affiliation: Techna Institute, University Health Network, 100 College Street, Toronto, Ontario, M5G 1P5, Canada; Princess Margaret Cancer Centre, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada; University of Toronto, Institute of Biomaterials and Biomedical Engineering, 164 College Street, Rm 407, Toronto, Ontario, M5S 3G9, Canada. Electronic address: jinzi.zheng@rmp.uhn.on.ca.
authors.forename: Jinzi
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Princess Margaret Cancer Centre, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada; University of Toronto, Department of Surgery, 149 College Street, 5th Floor, Toronto, Ontario, M5T 1P5, Canada.
authors.forename: Nidal
authors.initials: N
authors.lastname: Muhanna
authors.affiliation: Princess Margaret Cancer Centre, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada; University of Toronto, Pharmaceutical Sciences, 144 College Street, Toronto, Ontario, M5S 3M2, Canada.
authors.forename: Raquel
authors.initials: R
authors.lastname: De Souza
authors.affiliation: University of Toronto, Department of Surgery, 149 College Street, 5th Floor, Toronto, Ontario, M5T 1P5, Canada; Toronto General Hospital, Division of Thoracic Surgery, 101 College Street, Toronto, Ontario, M5G 1L7, Canada.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Wada
authors.affiliation: Techna Institute, University Health Network, 100 College Street, Toronto, Ontario, M5G 1P5, Canada; Princess Margaret Cancer Centre, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada.
authors.forename: Harley
authors.initials: H
authors.lastname: Chan
authors.affiliation: Techna Institute, University Health Network, 100 College Street, Toronto, Ontario, M5G 1P5, Canada; University of Toronto, Department of Surgery, 149 College Street, 5th Floor, Toronto, Ontario, M5T 1P5, Canada.
authors.forename: Margarete K
authors.initials: MK
authors.lastname: Akens
authors.affiliation: University of Toronto, Department of Surgery, 149 College Street, 5th Floor, Toronto, Ontario, M5T 1P5, Canada; Toronto General Hospital, Division of Thoracic Surgery, 101 College Street, Toronto, Ontario, M5G 1L7, Canada.
authors.forename: Takashi
authors.initials: T
authors.lastname: Anayama
authors.affiliation: Techna Institute, University Health Network, 100 College Street, Toronto, Ontario, M5G 1P5, Canada; University of Toronto, Department of Surgery, 149 College Street, 5th Floor, Toronto, Ontario, M5T 1P5, Canada; Toronto General Hospital, Division of Thoracic Surgery, 101 College Street, Toronto, Ontario, M5G 1L7, Canada; University of Toronto, Institute of Biomaterials and Biomedical Engineering, 164 College Street, Rm 407, Toronto, Ontario, M5S 3G9, Canada.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yasufuku
authors.affiliation: Toronto General Hospital, Department of Pathology, 200 Elizabeth Street, 11th Floor, Eaton Wing, Toronto, Ontario, M5G 2C4, Canada.
authors.forename: Stefano
authors.initials: S
authors.lastname: Serra
authors.affiliation: Techna Institute, University Health Network, 100 College Street, Toronto, Ontario, M5G 1P5, Canada; Princess Margaret Cancer Centre, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada; University of Toronto, Department of Surgery, 149 College Street, 5th Floor, Toronto, Ontario, M5T 1P5, Canada.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Irish
authors.affiliation: University of Toronto, Pharmaceutical Sciences, 144 College Street, Toronto, Ontario, M5S 3M2, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Allen
authors.affiliation: Techna Institute, University Health Network, 100 College Street, Toronto, Ontario, M5G 1P5, Canada; Princess Margaret Cancer Centre, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada; University of Toronto, Institute of Biomaterials and Biomedical Engineering, 164 College Street, Rm 407, Toronto, Ontario, M5S 3G9, Canada; University of Toronto, Medical Biophysics, 610 University Avenue, Rm 7-411, Toronto, Ontario, M5G 1L7, Canada; University of Toronto, Radiation Oncology, 150 College Street, Rm 106, Toronto, Ontario, M5S 3E2, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Jaffray
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology / Q000556:secondary; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005453:Fluorescence; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D016513:Mice, SCID; D064847:Multimodal Imaging; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000601:surgery*; D010051:Ovarian Neoplasms / Q000473:pathology; D011817:Rabbits; D025321:Surgery, Computer-Assisted; D014057:Tomography, X-Ray Computed
chemical_list: D008081:Liposomes
keywords: Cancer; Computed tomography; Image-guided surgery; Liposomes; Multimodality imaging; Nearinfrared fluorescence