title: Diversity of endophytic and rhizoplane bacterial communities associated with exotic Spartina alterniflora and native mangrove using Illumina amplicon sequencing.
abstract: Root-associated microbial communities are very important for biogeochemical cycles in wetland ecosystems and help to elaborate the mechanisms of plant invasions. In the estuary of Jiulong River (China), Spartina alterniflora has widely invaded Kandelia obovata-dominated habitats, offering an opportunity to study the influence of root-associated bacteria. The community structures of endophytic and rhizosphere bacteria associated with selected plant species were investigated using the barcoded Illumina paired-end sequencing technique. The diversity indices of bacteria associated with the roots of S. alterniflora were higher than those of the transition stands and K. obovata monoculture. Using principal coordinate analysis with UniFrac metrics, the comparison of β-diversity showed that all samples could be significantly clustered into 3 major groups, according to the bacteria communities of origin. Four phyla, namely Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes, were enriched in the rhizoplane of both salt marsh plants, while they shared higher abundances of Cyanobacteria and Proteobacteria among endophytic bacteria. Members of the phyla Spirochaetes and Chloroflexi were found among the endophytic bacteria of S. alterniflora and K. obovata, respectively. One of the interesting findings was that endophytes were more sensitive in response to plant invasion than were rhizosphere bacteria. With linear discriminate analysis, we found some predominant rhizoplane and endophytic bacteria, including Methylococcales, Pseudoalteromonadacea, Clostridium, Vibrio, and Desulfovibrio, which have the potential to affect the carbon, nitrogen, and sulfur cycles. Thus, the results provide clues to the isolation of functional bacteria and the effects of root-associated microbial groups on S. alterniflora invasions.
authors.affiliation: a Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, People's Republic of China.|b Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
authors.forename: Youwei
authors.initials: Y
authors.lastname: Hong
authors.affiliation: c Xiamen Huaxia College, Xiamen 361024, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liao
authors.affiliation: a Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, People's Republic of China.
authors.forename: Anyi
authors.initials: A
authors.lastname: Hu
authors.affiliation: d College of Ecology and Resources Engineering, Wuyi University, Wuyishan City 354300, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, People's Republic of China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Chen
authors.affiliation: e Department of Environmental Sciences, University of Peshawar, 25120, Pakistan.
authors.forename: Sardar
authors.initials: S
authors.lastname: Khan
authors.affiliation: a Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, People's Republic of China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Su
authors.affiliation: a Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, People's Republic of China.|f University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D002681:China; D017753:Ecosystem; D060026:Endophytes; D063366:Estuaries; D019015:Geologic Sediments / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology*; D006109:Poaceae / Q000382:microbiology*; D043982:Rhizophoraceae / Q000382:microbiology*; D058441:Rhizosphere; D045483:Rivers; D053833:Wetlands
keywords: Illumina amplicon sequencing; Spartina alterniflora; bactéries endophytes; diversity; diversité; endophytic bacteria; mangrove; séquençage d’amplicons sur plateforme Illumina