title: Application of HPLC-DAD after SPE/QuEChERS with ZrO2-based sorbent in d-SPE clean-up step for pesticide analysis in edible oils.
abstract: In this study, the solid-phase extraction/quick, easy, cheap, effective, rugged and safe (SPE/QuEChERS) technique was adapted to develop a simple sample treatment for multi-residue pesticide analysis of edible oils. The proposed method is based on liquid-liquid partitioning with acetonitrile followed by dispersive solid phase extraction using zirconia-coated silica particles for extract purification. To evaluate the described method, 21 pesticides belonging to different chemical classes were analysed using high performance liquid chromatography with diode-array detection (HPLC-DAD). For validation purposes, recovery studies were performed at 75 ng g(-1), 125 ng g(-1), 250 ng g(-1), 500 ng g(-1) and 1000 ng g(-1) levels. Recoveries were over the range of 50-130% for most of the analytes, with relative standard deviations less than 15% being observed. HPLC-DAD provided suitable linearity, precision and accuracy. The validated method was successfully applied to the analysis of edible oil samples selected from the market.
authors.affiliation: Medical University in Lublin, Faculty of Pharmacy with Medical Analytics Division, Chair of Chemistry, Department of Physical Chemistry, 4A Chodźki Street, 20-093 Lublin, Poland. Electronic address: tomasz.tuzimski@umlub.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Tuzimski
authors.affiliation: Medical University in Lublin, Faculty of Pharmacy with Medical Analytics Division, Chair of Chemistry, Department of Physical Chemistry, 4A Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rejczak
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009821:Oils / Q000032:analysis / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D009821:Oils; D010575:Pesticides
keywords: Dispersive-SPE; Edible oils; HPLC–DAD; Matrix effect; Pesticides; QuEChERS; Solid phase extraction (SPE); Zirconium dioxide-based sorbent