title: Complete genome sequences and comparative genome analysis of Lactobacillus plantarum strain 5-2 isolated from fermented soybean.
abstract: Lactobacillus plantarum is an important probiotic and is mostly isolated from fermented foods. We sequenced the genome of L. plantarum strain 5-2, which was derived from fermented soybean isolated from Yunnan province, China. The strain was determined to contain 3114 genes. Fourteen complete insertion sequence (IS) elements were found in 5-2 chromosome. There were 24 DNA replication proteins and 76 DNA repair proteins in the 5-2 genome. Consistent with the classification of L. plantarum as a facultative heterofermentative lactobacillus, the 5-2 genome encodes key enzymes required for the EMP (Embden-Meyerhof-Parnas) and phosphoketolase (PK) pathways. Several components of the secretion machinery are found in the 5-2 genome, which was compared with L. plantarum ST-III, JDM1 and WCFS1. Most of the specific proteins in the four genomes appeared to be related to their prophage elements.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Chen-Jian
authors.initials: CJ
authors.lastname: Liu
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Fu-Ming
authors.initials: FM
authors.lastname: Gong
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Liu
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China.
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Zheng
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Yi-Yong
authors.initials: YY
authors.lastname: Luo
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: starkeyran@163.com.
authors.forename: Xiao-Ran
authors.initials: XR
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D019143:Evolution, Molecular; D005285:Fermentation; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000235:genetics* / Q000821:virology; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D039002:Prophages / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA / Q000379:methods*; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements
keywords: Bacteria; Genome; Lactobacillus plantarum