title: A Dual Receptor and Reporter for Multi-Modal Cell Surface Engineering.
abstract: The rapid development of new small molecule drugs, nanomaterials, and genetic tools to modulate cellular function through cell surface manipulation has revolutionized the diagnosis, study, and treatment of disorders in human health. Since the cell membrane is a selective gateway barrier that serves as the first line of defense/offense and communication to its environment, new approaches that molecularly engineer or tailor cell membrane surfaces would allow for a new era in therapeutic design, therapeutic delivery, complex coculture tissue construction, and in situ imaging probe tracking technologies. In order to develop the next generation of multimodal therapies, cell behavior studies, and biotechnologies that focus on cell membrane biology, new tools that intersect the fields of chemistry, biology, and engineering are required. Herein, we develop a liposome fusion and delivery strategy to present a novel dual receptor and reporter system at cell surfaces without the use of molecular biology or metabolic biosynthesis. The cell surface receptor is based on bio-orthogonal functional groups that can conjugate a range of ligands while simultaneously reporting the conjugation through the emission of fluorescence. We demonstrate this dual receptor and reporter system by conjugating and tracking various cell surface ligands for temporal control of cell fluorescent signaling, cell-cell interaction, and tissue assembly construction.
authors.affiliation: Department of Chemistry, York University , Toronto, Ontario M3J 1P3 Canada.|Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
authors.forename: Nathan
authors.initials: N
authors.lastname: Westcott
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
authors.forename: Debjit
authors.initials: D
authors.lastname: Dutta
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
authors.forename: Abigail
authors.initials: A
authors.lastname: Pulsipher
authors.affiliation: Department of Chemistry, York University , Toronto, Ontario M3J 1P3 Canada.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Rogozhnikov
authors.affiliation: Department of Chemistry, York University , Toronto, Ontario M3J 1P3 Canada.
authors.forename: Jean
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemistry, York University , Toronto, Ontario M3J 1P3 Canada.|Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D015202:Protein Engineering; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008081:Liposomes; D011956:Receptors, Cell Surface