title: Molecular characterizations of two grapevine rupestris stem pitting-associated virus isolates from China.
abstract: The complete nucleotide sequences of two isolates of grapevine rupestris stem pitting-associated virus (LSL and JF) collected from grapevine of Xingcheng in Liaoning Province, China, were determined. The genomes of both LSL and JF were found to contain five open reading frames (ORFs). Sequence alignments showed that the genomic sequences of JF were 76.1 %-83.5 % identical to the other ten GRSPaV isolates that have been reported previously and that the nucleotide sequence identity of isolate LSL to other isolates was no more than 78 %. Phylogenetic analysis based on the complete genome sequence indicated that JF belongs to group III and that LSL belongs to a new group (group IV). The average genetic distances of the new genetic lineage from groups I, II and III were 0.34, 0.32 and 0.33, respectively.
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China. hugj3114@163.com.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Hu
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China. yfdong@163.com.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Dong
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Zhu
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.
authors.forename: Zun-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Fan
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ren
authors.affiliation: National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D002681:China; D052677:Flexiviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D027843:Vitis / Q000821:virology*