title: Quantitative disease resistance to the bacterial pathogen Xanthomonas campestris involves an Arabidopsis immune receptor pair and a gene of unknown function.
abstract: Although quantitative disease resistance (QDR) is a durable and broad-spectrum form of resistance in plants, the identification of the genes underlying QDR is still in its infancy. RKS1 (Resistance related KinaSe1) has been reported recently to confer QDR in Arabidopsis thaliana to most but not all races of the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc). We therefore explored the genetic bases of QDR in A. thaliana to diverse races of X. campestris (Xc). A nested genome-wide association mapping approach was used to finely map the genomic regions associated with QDR to Xcc12824 (race 2) and XccCFBP6943 (race 6). To identify the gene(s) implicated in QDR, insertional mutants (T-DNA) were selected for the candidate genes and phenotyped in response to Xc. We identified two major QTLs that confer resistance specifically to Xcc12824 and XccCFBP6943. Although QDR to Xcc12824 is conferred by At5g22540 encoding for a protein of unknown function, QDR to XccCFBP6943 involves the well-known immune receptor pair RRS1/RPS4. In addition to RKS1, this study reveals that three genes are involved in resistance to Xc with strikingly different ranges of specificity, suggesting that QDR to Xc involves a complex network integrating multiple response pathways triggered by distinct pathogen molecular determinants.
authors.affiliation: Laboratoire Génétique et Evolution des Populations Végétales, UMR CNRS 8198, Université des Sciences et Technologies de Lille-Lille 1, Villeneuve d'Ascq cedex, F-59655, France.|INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, F-31326, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Marilyne
authors.initials: M
authors.lastname: Debieu
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, F-31326, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Huard-Chauveau
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, F-31326, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Genissel
authors.affiliation: Laboratoire Génétique et Evolution des Populations Végétales, UMR CNRS 8198, Université des Sciences et Technologies de Lille-Lille 1, Villeneuve d'Ascq cedex, F-59655, France.|INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, F-31326, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Roux
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, F-31326, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Roby
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040641:Quantitative Trait Loci / Q000235:genetics; D011956:Receptors, Cell Surface; D016959:Xanthomonas campestris / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; C582898:RRS1 protein, Arabidopsis; D011956:Receptors, Cell Surface; C097421:rps4 protein, plant
keywords: Arabidopsis thaliana; GWA mapping; RRS1/RPS4; Xanthomonas campestris; mutant analysis; quantitative disease resistance