title: Continuous hydrolysis of carboxymethyl cellulose with cellulase aggregates trapped inside membranes.
abstract: Enzymatic hydrolysis of cellulose is often conducted in batch processes in which hydrolytic products tend to inhibit enzyme activity. In this study, we report a method for continuous hydrolysis of carboxymethyl cellulose (CMC) by using cross-linked cellulase aggregate (XCA) trapped inside a membrane. XCA particles prepared by using a millifluidic reactor have a uniform size distribution around 350 nm. Because of their large size, XCA particles in solutions can be filtered through a polyethersulfone membrane to collect 87.1 ± 0.9% of XCA particles. The membrane with impregnated XCA can be used as a catalyst for hydrolysis of CMC in a continuous mode. When the CMC concentration is 1.0 g/l and the flow rate is 2 μl/min, 53.9% of CMC is hydrolyzed to reducing sugars. The membrane with XCA is very stable under continuously flowing solutions. After 72 h of reaction, 97.5% of XCA remains inside the membrane.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117576 Singapore.
authors.forename: Le Truc
authors.initials: le T
authors.lastname: Nguyen
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117576 Singapore.
authors.forename: Kristyn Rui Shan
authors.initials: KR
authors.lastname: Neo
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117576 Singapore. Electronic address: cheyk@nus.edu.sg.
authors.forename: Kun-Lin
authors.initials: KL
authors.lastname: Yang
mesh_terms: D019149:Bioreactors; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism*; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D006868:Hydrolysis; D056656:Lab-On-A-Chip Devices; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D010316:Particle Size
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D046912:Multiprotein Complexes; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium
keywords: Bioreactor; Cellulase; Enzyme aggregate; Enzyme inhibition; Hydrolysis; Membrane