title: The Impact of Systemic and Local Administration of Ascorbic Acid on Traumatic Perforation of Tympanic Membrane and Myringosclerosis.
abstract: OBJECTIVE
  In the present study, tympanic membranes (TM) harvested from myringotomized rats were analyzed histopathologically to compare the systemic and local effects of ascorbic acid on the development of myringosclerosis.
  
  
  METHODS
  Forty male Wistar-Albino rats weighing between 350-400 g were included in this study. Under otomicroscopic examination, a standard 2-mm myringotomy incision was made on the posteroinferior quadrant of the TM of both ears. Rats were randomized into five groups as control, topical ascorbic acid 50 mg/kg, systemic ascorbic acid 50, 100, and 200 mg/kg groups, each group containing eight rats. On the 15th day of the study, the rats were decapitated, and bullas of the rats were extracted. Sections were stained with hematoxylin-eosin and examined through light microscopy. Inflammation, distribution width of plaques, edema, and neovascularization were observed on the lamina propria. Thickness of the TM was evaluated under the microscope and scored semiquantitatively.
  
  
  RESULTS
  When intergroup comparisons of parameters related to total TM thickness were performed, differences between the control group and topical AA (ascorbic acid) or systemic treatment groups were found to be statistically significant (p<0.005). A statistically significant difference was detected among control, topical and systemic 200 mg/kg ascorbic acid groups for the edematous lamina propria (p=0.003 and p<0.05, respectively).
  
  
  CONCLUSIONS
  For the total TM thickness, systemic and topical ascorbic acid use was effective when compared with the control group. It has been concluded that systemic use of higher doses of (200 mg/kg) ascorbic acid is beneficial in the resolution of the edematous lamina propria.
authors.affiliation: Department of Otorhinolaryngology, Gölbaşı Hasvak State Hospital, Ankara, Turkey; akif_gunes@hotmail.com.
authors.forename: Akif
authors.initials: A
authors.lastname: Güneş
authors.forename: Murad
authors.initials: M
authors.lastname: Mutlu
authors.forename: İstemihan
authors.initials: İ
authors.lastname: Akın
authors.forename: Fulya
authors.initials: F
authors.lastname: Köybaşioğlu
authors.forename: Ali
authors.initials: A
authors.lastname: Güvey
authors.forename: Mehmet Fatih
authors.initials: MF
authors.lastname: Karasu
authors.forename: Hasan
authors.initials: H
authors.lastname: Albasan
authors.forename: Halime
authors.initials: H
authors.lastname: Cengiz
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001205:Ascorbic Acid / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008297:Male; D063371:Myringosclerosis / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D014432:Tympanic Membrane / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D018058:Tympanic Membrane Perforation / Q000188:drug therapy* / Q000209:etiology; D014947:Wounds and Injuries / Q000150:complications
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid