title: Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment.
abstract: As it became evident recently, extracellular DNA could be a versatile nutrient source of the facultative pathogen Vibrio cholerae along the different stages of its life cycle. By the use of two extracellular nucleases and periplasmic phosphatases, V. cholerae degrades extracellular DNA to nucleosides. In this study, we investigated the nucleoside uptake via identification and characterization of VCA0179, VC1953 and VC2352 representing the three nucleoside transport systems in V. cholerae. Based on our results VC2352 seems to be the dominant nucleoside transporter. Nevertheless, all three transporters are functional and can contribute to the utilization of nucleosides as a sole source of carbon or nitrogen. We found that the transcriptional activity of these three distal genes is equally promoted or antagonized by CRP or CytR respectively. Finally, mutants impaired for nucleoside uptake exhibit decreased transition fitness from the host into low carbon environments along the life cycle of V. cholerae.
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Tanja
authors.initials: T
authors.lastname: Gumpenberger
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Dina
authors.initials: D
authors.lastname: Vorkapic
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Franz G
authors.initials: FG
authors.lastname: Zingl
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Pressler
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Lackner
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Seper
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Joachim
authors.initials: J
authors.lastname: Reidl
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstr. 50, Graz, 8010, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schild
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D002771:Cholera / Q000378:metabolism / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D033703:Nucleoside Transport Proteins / Q000235:genetics / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*; D014734:Vibrio cholerae / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D033703:Nucleoside Transport Proteins; D009705:Nucleosides