title: Functional Loss of Bmsei Causes Thermosensitive Epilepsy in Contractile Mutant Silkworm, Bombyx mori.
abstract: The thermoprotective mechanisms of insects remain largely unknown. We reported the Bombyx mori contractile (cot) behavioral mutant with thermo-sensitive seizures phenotype. At elevated temperatures, the cot mutant exhibit seizures associated with strong contractions, rolling, vomiting, and a temporary lack of movement. We narrowed a region containing cot to ~268 kb by positional cloning and identified the mutant gene as Bmsei which encoded a potassium channel protein. Bmsei was present in both the cell membrane and cytoplasm in wild-type ganglia but faint in cot. Furthermore, Bmsei was markedly decreased upon high temperature treatment in cot mutant. With the RNAi method and injecting potassium channel blockers, the wild type silkworm was induced the cot phenotype. These results demonstrated that Bmsei was responsible for the cot mutant phenotype and played an important role in thermoprotection in silkworm. Meanwhile, comparative proteomic approach was used to investigate the proteomic differences. The results showed that the protein of Hsp-1 and Tn1 were significantly decreased and increased on protein level in cot mutant after thermo-stimulus, respectively. Our data provide insights into the mechanism of thermoprotection in insect. As cot phenotype closely resembles human epilepsy, cot might be a potential model for the mechanism of epilepsy in future.
authors.affiliation: 1] State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China [2] the Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China [3] College of Bio-Technology, Southwest University, Chongqing 400716, China [4] College of Bee Science, Fujian Agriculture and Forestry University, Fujian Fuzhou 350002, China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Nie
authors.affiliation: 1] State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China [2] the Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China [3] College of Bio-Technology, Southwest University, Chongqing 400716, China.
authors.forename: Tingcai
authors.initials: T
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China.
authors.forename: Yinxia
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1] State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China [2] the Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China [3] College of Bio-Technology, Southwest University, Chongqing 400716, China.
authors.forename: Fangyin
authors.initials: F
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China.
authors.forename: Kazuei
authors.initials: K
authors.lastname: Mita
authors.affiliation: 1] State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China [2] the Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China [3] College of Bio-Technology, Southwest University, Chongqing 400716, China.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
authors.affiliation: 1] State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China [2] the Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China [3] College of Bio-Technology, Southwest University, Chongqing 400716, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002462:Cell Membrane / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics; D004827:Epilepsy / Q000235:genetics*; D006358:Hot Temperature; D019476:Insect Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D012640:Seizures / Q000235:genetics
chemical_list: D019476:Insect Proteins