title: Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity.
abstract: We developed a one-pot label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for simple, rapid and sensitive DNA methyltransferase (MTase) activity detection.
authors.affiliation: School of Pharmacy, Shandong University, Jinan 250012, P. R. China. wangl-sdu@sdu.edu.cn.
authors.forename: Qingwang
authors.initials: Q
authors.lastname: Xue
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D019175:DNA Methylation; D004591:Electrophoresis, Polyacrylamide Gel; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D057230:Limit of Detection; D008780:Methyltransferases / Q000737:chemistry / Q000378:metabolism*; D015335:Molecular Probes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D008780:Methyltransferases