title: A yeast co-culture-based biosensor for determination of waste water contamination levels.
abstract: Artificial microbial co-cultures were formed to develop the receptor element of a biosensor for assessment of biological oxygen demand (BOD). The co-cultures possessed broad substrate specificities and enabled assays of water and fermentation products within a broad BOD range (2.4-80 mg/dm(3)) with a high correlation to the standard method (R = 0.9988). The use of the co-cultures of the yeasts Pichia angusta, Arxula adeninivorans and Debaryomyces hansenii immobilized in N-vinylpyrrolidone-modified poly(vinyl alcohol) enabled developing a BOD biosensor possessing the characteristics not inferior to those in the known biosensors. The results are indicative of a potential of using these co-cultures as the receptor element base in prototype models of instruments for broad application.
authors.affiliation: Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia.
authors.forename: N Yu
authors.initials: NY
authors.lastname: Yudina
authors.affiliation: Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Arlyapov
authors.affiliation: Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Chepurnova
authors.affiliation: Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Alferov
authors.affiliation: Federal State Budgetary Institution of Science "G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms", Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region 142290, Russia; Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia. Electronic address: anatol@ibpm.pushchino.ru.
authors.forename: A N
authors.initials: AN
authors.lastname: Reshetilov
mesh_terms: D057919:Biological Oxygen Demand Analysis / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D002175:Candida / Q000378:metabolism; D018914:Cells, Immobilized / Q000378:metabolism; D018920:Coculture Techniques; D055165:Debaryomyces / Q000378:metabolism; D005285:Fermentation; D020600:Gluconobacter oxydans / Q000378:metabolism; D010843:Pichia / Q000378:metabolism; D004718:Saccharomycetales / Q000378:metabolism; D062065:Wastewater / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bacteria; Biochemical oxygen demand (BOD); Biosensor analyzer; Co-culture; Growth curves; Yeasts