title: A novel electrochemical aptasensor for ultrasensitive detection of kanamycin based on MWCNTs-HMIMPF6 and nanoporous PtTi alloy.
abstract: A novel aptasensor based on a novel composite film consisting of multi-walled carbon nanotubes (MWCNTs), ionic liquid (IL) of 1-hexyl-3-methylimidazolium hexafluorophosphate (HMIMPF6), and nanoporous PtTi (NP-PtTi) alloy was constructed for ultrasensitive detection of kanamycin. The NP-PtTi alloy was successfully fabricated by a simple dealloying of PtTiAl source alloy in HCl solution. The NP-PtTi alloy has uniform interconnected network structure with specific surface area and was used to immobilize aptamer. After modified with the composite material, current signal was amplified obviously, which attributed to the larger specific surface area and excellent electrical conductivity of NP-PtTi and MWCNTs. A number of factors affecting the activity of the aptasensor have been discussed and optimized. Under the optimized conditions, the proposed aptasensor provided a linear range of 0.05-100 ng mL(-1) with a low detection limit of 3.7 pg mL(-1). This aptasensor displayed high sensitivity, stability and reproducibility. In addition, the as-prepared aptasensor was successfully used for the determination of kanamycin in a real sample.
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Environmental Protection Monitoring Station, Jining 272045, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qin
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: chm_peims@ujn.edu.cn.
authors.forename: Meishan
authors.initials: M
authors.lastname: Pei
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Luyan
authors.initials: L
authors.lastname: Wang
mesh_terms: D000497:Alloys / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D007093:Imidazoles / Q000737:chemistry; D007612:Kanamycin / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D058608:Nanopores / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014025:Titanium / Q000737:chemistry
chemical_list: C516814:1,3-dimethylimidazolium; D000497:Alloys; D052157:Aptamers, Nucleotide; D007093:Imidazoles; D037742:Nanotubes, Carbon; D010984:Platinum; D007612:Kanamycin; D014025:Titanium
keywords: Aptasensor; Ionic liquid; Kanamycin; Nanoporous; Platinum