title: The effect of equine-derived bone protein extract (Colloss-E) in the treatment of cavitary bone defects: an experimental study.
abstract: OBJECTIVE
  Bone protein extract (BPE) usually requires a carrier or a scaffold for implantation. We aimed to compare the effect of equine-derived BPE, an osteoinductive agent composed of a high amount of type-I collagen and other bone proteins (Colloss-E), with that of human demineralized bone matrix (DBM) for treating cavitary bone defects not requiring scaffold use.
  
  
  METHODS
  Rabbit distal femoral condyle was used as a stable cavitary bone defect model. Bone defects of 6-mm diameter and 10-12-mm depth were created in the femoral condyles. Rabbits were assigned into the equine-derived BPE (BPE), human-derived DBM (DBM), and control (C) groups. Approximately 20 mg of BPE was implanted into the defect in the equine-derived BPE group (n=6), whereas 0.3 cc of DBM was implanted in the DBM group (n=6). Defects were left empty in the C group (n=6). The defect area was histologically examined after 6 weeks.
  
  
  RESULTS
  There were no instances of macroscopic defect collapse or failure. Histopathological examination revealed that the BPE group had better scores (statistically significant) than both the other groups in terms of quality of union. The BPE group also had higher scores than the DBM group in terms of graft incorporation and new-bone formation.
  
  
  CONCLUSIONS
  The current study revealed results consistent with those of the previous studies concerning BPEs. Equine-derived BPE was found to be successful for treating cavitary bone defects not requiring scaffold use.
authors.affiliation: Bülent Ecevit University Faculty of Medicine, Department of Orthopaedics and Traumatology, Zonguldak, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Songür
authors.affiliation: Bülent Ecevit University Faculty of Medicine, Department of Orthopaedics and Traumatology, Zonguldak, Turkey.
authors.forename: Ercan
authors.initials: E
authors.lastname: Şahin
authors.affiliation: Bahçeşehir University, Medical Park Hospital, Department of Orthopaedics and Traumatology, İstanbul, Turkey.
authors.forename: Tuğcan
authors.initials: T
authors.lastname: Demir
authors.affiliation: Ankara University Faculty of Medicine, Department of Orthopaedics and Traumatology, Ankara, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Kalem
authors.affiliation: Gazi University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey.
authors.forename: Gülnur
authors.initials: G
authors.lastname: Take Kaplanoğlu
authors.affiliation: Gazi University Faculty of Medicine, Department of Orthopaedics and Traumatology, Ankara, Turkey.
authors.forename: Necdet Şükrü
authors.initials: NŞ
authors.lastname: Altun
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001857:Bone Matrix / Q000637:transplantation*; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation / Q000379:methods; D003094:Collagen / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005269:Femur / Q000473:pathology*; D006736:Horses; D006801:Humans; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; C515502:COLLOSS E; D003094:Collagen