title: MicroRNA-26a Promotes Regulatory T cells and Suppresses Autoimmune Diabetes in Mice.
abstract: Type-1 diabetes (TID) is an autoimmune disease in which the body's own immune cells attack islet β cells, the cells in the pancreas that produce and release the hormone insulin. Mir-26a has been reported to play functions in cellular differentiation, cell growth, cell apoptosis, and metastasis. However, the role of microRNA-26a (Mir-26a) in autoimmune TID has never been investigated. In our current study, we found that pre-Mir-26a (LV-26a)-treated mice had significantly longer normoglycemic time and lower frequency of autoreactive IFN-γ-producing CD4(+) cells compared with an empty lentiviral vector (LV-Con)-treated non-obese diabetic (NOD) mice. Mir-26a suppresses autoreactive T cells and expands Tregs in vivo and in vitro. Furthermore, in our adoptive transfer study, the groups receiving whole splenocytes and CD25-depleted splenocytes from LV-Con-treated diabetic NOD mice develop diabetes at 3 to 4 weeks of age. In comparison, mice injected with undepleted splenocytes obtained from LV-26a-treated reversal NOD mice develop diabetes after 6-8 weeks. And depletion of CD25(+) cells in the splenocytes of reversed mice abrogates the delay in diabetes onset. In conclusion, Mir-26a suppresses autoimmune diabetes in NOD mice in part through promoted regulatory T cells (Tregs) expression.
authors.affiliation: Department of Geriatrics, Affiliated Hospital of Binzhou Medical University, No.661, Section 2, The Yellow River Road, Binzhou, Shandong, 256603, China. mahui0001@yeah.net.
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, China.
authors.forename: Shoutao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, Affiliated Hospital of Binzhou Medical University, No.661, Section 2, The Yellow River Road, Binzhou, Shandong, 256603, China.
authors.forename: Doufei
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Geriatrics, Affiliated Hospital of Binzhou Medical University, No.661, Section 2, The Yellow River Road, Binzhou, Shandong, 256603, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Geriatrics, Affiliated Hospital of Binzhou Medical University, No.661, Section 2, The Yellow River Road, Binzhou, Shandong, 256603, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Cui
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D006943:Hyperglycemia / Q000235:genetics / Q000517:prevention & control*; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007515:Islets of Langerhans / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000637:transplantation
chemical_list: C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D035683:MicroRNAs; C543693:Mirn26 microRNA, mouse; D007371:Interferon-gamma
keywords: NOD mice; microRNA-26a; regulatory T cells; type-1 diabetes