title: Fingerprint analysis, multi-component quantitation, and antioxidant activity for the quality evaluation of Salvia miltiorrhiza var. alba by high-performance liquid chromatography and chemometrics.
abstract: Salvia miltiorrhiza Bge. var. alba C.Y. Wu and H.W. Li has wide prospects in clinical practice. A useful comprehensive method was developed for the quality evaluation of S. miltiorrhiza var. alba by three quantitative parameters: high-performance liquid chromatography fingerprint, ten-component contents, and antioxidant activity. The established method was validated for linearity, precision, repeatability, stability, and recovery. Principal components analysis and hierarchical clustering analysis were both used to evaluate the quality of the samples from different origins. The results showed that there were category discrepancies in quality of S. miltiorrhiza var. alba samples according to the three quantitative parameters. Multivariate linear regression was adopted to explore the relationship between components and antioxidant activity. Three constituents, namely, danshensu, rosmarinic acid, and salvianolic acid B, significantly correlated with antioxidant activity, and were successfully elucidated by the optimized multivariate linear regression model. The combined use of high-performance liquid chromatography fingerprint analysis, simultaneous multicomponent quantitative analysis, and antioxidant activity for the quality evaluation of S. miltiorrhiza var. alba is a reliable, comprehensive, and promising approach, which might provide a valuable reference for other herbal products in general to improve their quality control.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, China.
authors.forename: Danlu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, China.
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Duan
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Deng
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Nie
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, China.|National Glycoengineering Research Center, Jinan, China.
authors.forename: Hengchang
authors.initials: H
authors.lastname: Zang
mesh_terms: D000975:Antioxidants / Q000032:analysis; D001572:Benzofurans / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D006801:Humans; D007773:Lactates / Q000032:analysis; D025341:Principal Component Analysis; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D007773:Lactates; D000097123:Rosmarinic Acid; C028972:dan-shen root extract; C035055:3,4-dihydroxyphenyllactic acid; C076944:salvianolic acid B
keywords: Antioxidant activity; Fingerprint; Quality control; Salvia miltiorrhiza var. alba; Traditional Chinese medicine