title: Interactions between breathing rate and low-frequency fluctuations in blood pressure and cardiac intervals.
abstract: Evidence derived from spontaneous measures of cardiovagal baroreflex sensitivity (BRS) suggests that slow breathing at 6 breaths/min augments BRS. However, increases in BRS associated with slow breathing may simply reflect the frequency-dependent nature of the baroreflex rather than the modulation of baroreflex function by changes in breathing rate per se. To test this hypothesis we employed a crossover study design (n = 14) wherein breathing rate and systolic arterial blood pressure (SAP) oscillation induced via the application of oscillating lower body negative pressure (OLBNP) were independently varied at fixed frequencies. Breathing rate was controlled at 6 or 10 breaths/min with the aid of a metronome, and SAP oscillations were driven at 0.06 Hz and 0.1 Hz using OLBNP. The magnitudes of SAP and R-R interval (cardiac period) oscillations were quantified using power spectral analysis, and the transfer function gain between SAP and R-R interval was used to estimate BRS. Linear mixed-effects models were used to examine the main effects and interactions between breathing rate and OLBNP frequency. There was no statistical interaction between breathing and OLBNP frequency (P = 0.59), indicating that the effect of breathing rate on BRS did not differ according to OLBNP frequency (and vice versa). Additionally, there was no main effect for breathing rate (P = 0.28). However, we observed a significant main effect for OLBNP frequency (P = 0.01) consistent with the frequency-dependent nature of baroreflex. These findings suggest that increases in spectral indices of BRS reflect the frequency dependence of the baroreflex and are not due to slow breathing per se.
authors.affiliation: Cardiovascular Systems Laboratory and Centre for Translational Physiology, University of Otago, Wellington, New Zealand;
authors.forename: H M
authors.initials: HM
authors.lastname: Horsman
authors.affiliation: Cardiovascular Systems Laboratory and Centre for Translational Physiology, University of Otago, Wellington, New Zealand; Department of Physiology, University of Otago, Dunedin, New Zealand; and Department of Health Professions, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
authors.forename: K C
authors.initials: KC
authors.lastname: Peebles
authors.affiliation: Cardiovascular Systems Laboratory and Centre for Translational Physiology, University of Otago, Wellington, New Zealand; shieak.tzeng@otago.ac.nz.
authors.forename: Y C
authors.initials: YC
authors.lastname: Tzeng
mesh_terms: D000328:Adult; D062186:Arterial Pressure / Q000502:physiology*; D017704:Baroreflex / Q000502:physiology; D002245:Carbon Dioxide / Q000097:blood; D018592:Cross-Over Studies; D004562:Electrocardiography; D005260:Female; D006339:Heart Rate / Q000502:physiology*; D006801:Humans; D016014:Linear Models; D008150:Lower Body Negative Pressure; D008297:Male; D011311:Pressoreceptors / Q000502:physiology; D056152:Respiratory Rate / Q000502:physiology*; D055815:Young Adult
chemical_list: D002245:Carbon Dioxide
keywords: baroreceptors; baroreflex sensitivity; blood pressure; breathing; heart rate