title: Myristoylation profiling in human cells and zebrafish.
abstract: Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Broncel
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Remigiusz A
authors.initials: RA
authors.lastname: Serwa
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Paulina
authors.initials: P
authors.lastname: Ciepla
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.affiliation: Department of Life Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Dallman
authors.affiliation: National Heart and Lung Institute, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Anthony I
authors.initials: AI
authors.lastname: Magee
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Edward W
authors.initials: EW
authors.lastname: Tate
keywords: Modification site; Myristoylation; Protein modification; Tagging by substrate