title: BIOENERGETIC CHARACTERIZATION OF H9C2 CELLS USING THE EXTRACELLULAR FLUX ANALYZER.
abstract: The aim of the present work was to standardize the working methodology for assessing the bioenergetic profile of H9c2 cardiomyoblasts cells, with reference to the optimization of cell seeding number and the establishment of favorable concentrations for the classic modulators of mitochondrial respiratory function, in particular the one of a classical uncoupler, FCCP.
  
  
  METHODS
  The extracellular flux analyzer (XF, Seahorse Bioscience) is a novel high-throughput instrument able to monitor the metabolism of living cells by simultaneously measuring mitochondrial respiration and glycolysis. The in vitro platform will be further used to better understand the pathophysiology and the unrecognized side effects of drugs currently used in the therapy of major cardiovascular diseases.
  
  
  CONCLUSIONS
  In the long run, characterization of novel pharmacological agents' effects on other cell lines, including tumoral ones, will be also considered.
authors.forename: Ileana
authors.initials: I
authors.lastname: Scurtu
authors.forename: A
authors.initials: A
authors.lastname: Sturza
authors.forename: Ioanna Zinuca
authors.initials: IZ
authors.lastname: Pavel
authors.forename: Roxana
authors.initials: R
authors.lastname: Popescu
authors.forename: Andreea
authors.initials: A
authors.lastname: Privistirescu
authors.forename: Oana Maria
authors.initials: OM
authors.lastname: Duicu
authors.forename: Danina Mirela
authors.initials: DM
authors.lastname: Muntean
mesh_terms: D000818:Animals; D002460:Cell Line; D004734:Energy Metabolism; D006019:Glycolysis; D064688:Metabolic Flux Analysis / Q000295:instrumentation / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D032386:Myoblasts, Cardiac / Q000378:metabolism*; D051381:Rats; D012015:Reference Standards