title: Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway.
abstract: Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated disease (PCVAD). Astragalus polysaccharide (APS), as one kind of biological macromolecule extracted from Astragalus, has antiviral activities. This study was undertaken to explore the effect of APS on PCV2 replication in vitro and the underlying mechanisms. Our results showed that adding APS before PCV2 infection decreased significantly PCV2 DNA copies, the number of infected cells, MDA level, ROS level and NF-κB activation in PK15 cells and increased significantly GSH contents and SOD activity compared to control without APS. Oxidative stress induced by BSO could eliminate the effect of PCV2 replication inhibition by APS. LPS, as a NF-κB activator, could attenuate the effect of PCV2 replication inhibition by APS. BAY 11-7082, as a NF-κB inhibitor, could increase the effect of PCV2 replication inhibition by APS. In conclusion, APS inhibits PCV2 replication by decreasing oxidative stress and the activation of NF-κB signaling pathway, which suggests that APS might be employed for the prevention of PCV2 infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: 2013107105@njau.edu.cn.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Xue
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: ganfang3211@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Gan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: 2013807128@njau.edu.cn.
authors.forename: Zheqian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: 2013107085@njau.edu.cn.
authors.forename: Junfa
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: cxx@njau.edu.cn.
authors.forename: Xingxiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: khhuang@njau.edu.cn.
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D027883:Astragalus Plant / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017925:Circovirus / Q000187:drug effects* / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013552:Swine; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D016328:NF-kappa B; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Astragalus polysaccharide; NF-κB; Oxidative stress; Porcine circovirus type 2