title: [The influence of autophagy-related genes about X-Ray on nasopharyngeal carcinoma CNE2 and CNE2/DDP cells].
abstract: OBJECTIVE
  To study the relationship between the radiotherapy resistance and autophagy. To provide a theoretiacal basis for drugs that regulate autophagy to improve radiotherapy sensitivity.
  
  
  METHODS
  Flow cytometry (FCM) was performed to analyze the distribution of the cell cycle of CNE2 and CNE2/DDP cells under the action of X radiation. The expression of autopagy-specific gene Beclin1 and microtubule-associated protein light chain 3β (MAPLC3β) in CNE2 and CNE2/DDP cells was determined by real time PCR and Immumofluorescence staining.
  
  
  RESULTS
  CNE2/DDP and their parental CNE2 cells produced the G2-M phase arrest under the action of X radiation. With the radiation dose increasing,The cells which in the G2-M phase were more and more (P<0. 05). The G2-M phase arrest in CNE2/DDP cells was more obvious than in CNE2 cells (P<0. 05). The expression of Beclin1 and MAPLC3β in CNE2 and CNE2/DDP cells increased under the action of X radiation. What's more, the raise was more and more obvious with the increase of the irradiation dose(P<0. 05). The expression levels of Beclin1 and MAPLC3β in CNE2/DDP was lower than that in CNE2 cells (P<0. 05).
  
  
  CONCLUSIONS
  Autophagic cell death may be the one manner of death in nasopharyngeal carcinoma CNE2 and CNE2/DDP cells under the action of X radiation. The radiation resistance of CNE2/DDP cells may be related to the low expression of autophagy-related genes.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Dewei
authors.initials: D
authors.lastname: Cui
authors.forename: Weihua
authors.initials: W
authors.lastname: Xu
authors.forename: Minglang
authors.initials: M
authors.lastname: Hui
authors.forename: Leifeng
authors.initials: L
authors.lastname: Liu
authors.forename: Haitao
authors.initials: H
authors.lastname: Qiu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Xiao
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D001343:Autophagy; D000071186:Beclin-1; D002277:Carcinoma; D002453:Cell Cycle; D045744:Cell Line, Tumor / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000235:genetics; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D011836:Radiation Tolerance; D014965:X-Rays
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; C502070:MAP1LC3B protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins