title: High-quality draft genome sequences of five anaerobic oral bacteria and description of Peptoanaerobacter stomatis gen. nov., sp. nov., a new member of the family Peptostreptococcaceae.
abstract: Here we report a summary classification and the features of five anaerobic oral bacteria from the family Peptostreptococcaceae. Bacterial strains were isolated from human subgingival plaque. Strains ACC19a, CM2, CM5, and OBRC8 represent the first known cultivable members of "yet uncultured" human oral taxon 081; strain AS15 belongs to "cultivable" human oral taxon 377. Based on 16S rRNA gene sequence comparisons, strains ACC19a, CM2, CM5, and OBRC8 are distantly related to Eubacterium yurii subs. yurii and Filifactor alocis, with 93.2 - 94.4 % and 85.5 % of sequence identity, respectively. The genomes of strains ACC19a, CM2, CM5, OBRC8 and AS15 are 2,541,543; 2,312,592; 2,594,242; 2,553,276; and 2,654,638 bp long. The genomes are comprised of 2277, 1973, 2325, 2277, and 2308 protein-coding genes and 54, 57, 54, 36, and 28 RNA genes, respectively. Based on the distinct characteristics presented here, we suggest that strains ACC19a, CM2, CM5, and OBRC8 represent a novel genus and species within the family Peptostreptococcaceae, for which we propose the name Peptoanaerobacter stomatis gen. nov., sp. nov. The type strain is strain ACC19a(T) (=HM-483(T); =DSM 28705(T); =ATCC BAA-2665(T)).
authors.affiliation: Northeastern University, 360 Huntington Avenue, Boston, MA USA.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Sizova
authors.affiliation: Northeastern University, 360 Huntington Avenue, Boston, MA USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Chilaka
authors.affiliation: Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA USA.
authors.forename: Ashlee M
authors.initials: AM
authors.lastname: Earl
authors.affiliation: Northeastern University, 360 Huntington Avenue, Boston, MA USA.
authors.forename: Sebastian N
authors.initials: SN
authors.lastname: Doerfert
authors.affiliation: Northeastern University, 360 Huntington Avenue, Boston, MA USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Muller
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD USA.
authors.forename: Manolito
authors.initials: M
authors.lastname: Torralba
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD USA.
authors.forename: Jamison M
authors.initials: JM
authors.lastname: McCorrison
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD USA.
authors.forename: A Scott
authors.initials: AS
authors.lastname: Durkin
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD USA.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Nelson
authors.affiliation: Northeastern University, 360 Huntington Avenue, Boston, MA USA.
authors.forename: Slava S
authors.initials: SS
authors.lastname: Epstein
keywords: Anaerobic oral bacteria; Human oral taxa; Peptoanaerobacter stomatis; Peptostreptococcaceae; Uncultivable bacteria