title: Acrylamide neurotoxicity on the cerebrum of weaning rats.
abstract: The mechanism underlying acrylamide-induced neurotoxicity remains controversial. Previous studies have focused on acrylamide-induced toxicity in adult rodents, but neurotoxicity in weaning rats has not been investigated. To explore the neurotoxic effect of acrylamide on the developing brain, weaning rats were gavaged with 0, 5, 15, and 30 mg/kg acrylamide for 4 consecutive weeks. No obvious neurotoxicity was observed in weaning rats in the low-dose acrylamide group (5 mg/kg). However, rats from the moderate- and high-dose acrylamide groups (15 and 30 mg/kg) had an abnormal gait. Furthermore, biochemical tests in these rats demonstrated that glutamate concentration was significantly reduced, and γ-aminobutyric acid content was significantly increased and was dependent on acrylamide dose. Immunohistochemical staining showed that in the cerebral cortex, γ-aminobutyric acid, glutamic acid decarboxylase and glial fibrillary acidic protein expression increased remarkably in the moderate- and high-dose acrylamide groups. These results indicate that in weaning rats, acrylamide is positively associated with neurotoxicity in a dose-dependent manner, which may correlate with upregulation of γ-aminobutyric acid and subsequent neuronal degeneration after the initial acrylamide exposure.
authors.affiliation: School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Tian
authors.affiliation: School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Ma
authors.affiliation: Xiqiao People's Hospital, Foshan, Guangdong Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.affiliation: Clinical Laboratory of First Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan Province, China.
authors.forename: Ting-Ye
authors.initials: TY
authors.lastname: Lou
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Liu
authors.affiliation: School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Li
keywords: cerebrum; cortex; glial fibrillary acidic protein; glutamate; glutamic acid decarboxylase; nerve regeneration; neural regeneration; neurotoxicity; organ index; weaning; γ-aminobutyric acid