title: Rats Prone to Obesity Under a High-Carbohydrate Diet have Increased Post-Meal CCK mRNA Expression and Characteristics of Rats Fed a High-Glycemic Index Diet.
abstract: We previously reported that rats prone to obesity exhibit an exaggerated increase in glucose oxidation and an exaggerated decline in lipid oxidation under a low-fat high-carbohydrate (LF/HC) diet. The aim of the present study was to investigate the mechanisms involved in these metabolic dysregulations. After a 1-week adaptation to laboratory conditions, 48 male Wistar rats were fed a LF/HC diet for 3 weeks. During weeks 2 and 3, glucose tolerance tests (GTT), insulin tolerance tests (ITT), and meal tolerance tests (MTT) were performed to evaluate blood glucose, plasma, and insulin. Glucose and lipid oxidation were also assayed during the GTT. At the end of the study, body composition was measured in all the rats, and they were classified as carbohydrate resistant (CR) or carbohydrate sensitive (CS) according to their adiposity. Before sacrifice, 24 of the 48 rats received a calibrated LF/HC meal. Liver, muscle, and intestine tissue samples were taken to measure mRNA expression of key genes involved in glucose, lipid, and protein metabolism. ITT, GTT, and MTT showed that CS rats were neither insulin resistant nor glucose intolerant, but mRNA expression of cholecystokinin (CCK) in the duodenum was higher and that of CPT1, PPARα, and PGC1α in liver were lower than in CR rats. From these results, we make the hypothesis that in CS rats, CCK increased pancreatic secretion, which may favor a quicker absorption of carbohydrates and consequently induces an enhanced inhibition of lipid oxidation in the liver, leading to a progressive accumulation of fat preferentially in visceral deposits. Such a mechanism may explain why CS rats share many characteristics observed in rats fed a high-glycemic index diet.
authors.affiliation: UMR914, CRNH-IdF, INRA, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Chaumontet
authors.affiliation: UMR914, CRNH-IdF, AgroParisTech, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Dalila
authors.initials: D
authors.lastname: Azzout-Marniche
authors.affiliation: UMR914, CRNH-IdF, INRA, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Anne
authors.initials: A
authors.lastname: Blais
authors.affiliation: UMR914, CRNH-IdF, INRA, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Chalvon-Dermersay
authors.affiliation: Chaire Aliment, Nutrition, Comportement Alimentaire (ANCA), AgroParisTech , Paris , France.
authors.forename: Nachiket A
authors.initials: NA
authors.lastname: Nadkarni
authors.affiliation: UMR914, CRNH-IdF, INRA, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Julien
authors.initials: J
authors.lastname: Piedcoq
authors.affiliation: UMR914, CRNH-IdF, INRA, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Fromentin
authors.affiliation: UMR914, CRNH-IdF, AgroParisTech, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.affiliation: UMR914, CRNH-IdF, INRA, Nutrition Physiology and Ingestive Behavior , Paris , France.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Even
keywords: CCK; dietary obesity; glucose; glucose tolerance test; indirect calorimetry; insulin; obesity prone; rat model