title: Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells.
abstract: Probing stem cell-biomaterial interactions is of great significance in both gaining profound understanding of stem cell biology and advancing tissue regeneration. In the present work, we developed a series of poly(ϵ-caprolactone) (PCL) films bearing distinct pendant small functional groups to study the effects of biomaterial substrate chemistry on stem cell behaviors. PCL films, bearing hydroxyl (OH), methyl (CH₃), carboxyl (COOH) and amino (NH₂), demonstrated varied surface properties, such as wettability, serum protein adsorption and surface topographical feature. In comparison with pristine PCL film, the adhesion of hMSCs on PCL-COOH, PCL-OH and PCLCO films was significantly promoted and cells slightly outgrew on PCL-NH₂ and PCL-COOH films. Most importantly, the tri-lineage differentiation of hMSCs varied on this series of PCL films, with the best osteogenesis achieved on PCL-NH₂ film, PCL and PCL-CH₃ films supporting the superior adipogenic differentiation and PCL-CH₃ film being the most favorable one for chondrogenesis. This study highlights the critical roles of surface chemistry in modulating the fates of MSCs and potentially provides a practical guidance in developing instructive tissue engineering scaffolds.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, Shanghai 200237, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, Shanghai 200237, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Meidong
authors.initials: M
authors.lastname: Lang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, Shanghai 200237, P. R. China. Electronic address: zhaoyangye@ecust.edu.cn.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, Shanghai 200237, P. R. China. Electronic address: wstan@ecust.edu.cn.
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Tan
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone
keywords: Cell fate; Mesenchymal stem cells; Poly(ϵ-caprolactone); Small functional groups; Tissue engineering