title: Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell.
abstract: As typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. In this study, we analyzed the protein component of the barnacle Amphibalanus (= Balanus) amphitrite shell using gel-based proteomics. The results revealed 52 proteins in the A. Amphitrite shell. Among them, 40 proteins were categorized into 11 functional groups based on KOG database, and the remaining 12 proteins were unknown. Besides the known proteins in barnacle shell (SIPC, carbonic anhydrase and acidic acid matrix protein), we also identified chorion peroxidase, C-type lectin-like domains, serine proteases and proteinase inhibitor proteins in the A. Amphitrite shell. The sequences of these proteins were characterized and their potential functions were discussed. Histology and DAPI staining revealed living cells in the shell, which might secrete the shell proteins identified in this study.
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, R. P. China.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Science, Sanya City, Hainan Province, 572000, P. R. China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: He
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, R. P. China.
authors.forename: Yue-Him
authors.initials: YH
authors.lastname: Wong
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Science, Shenzhen University, Shenzhen, 518060, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Science, Shenzhen University, Shenzhen, 518060, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, R. P. China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D000595:Amino Acid Sequence; D060105:Animal Shells / Q000033:anatomy & histology / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000378:metabolism; D006244:Hardness; D008969:Molecular Sequence Data; D040901:Proteomics; D001468:Thoracica / Q000033:anatomy & histology* / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins