title: Does the presence of sucrose in pediatric antibiotics influence the enamel mineral loss and the Streptococcus mutans counts in dental biofilm?
abstract: The role of antibiotics containing sucrose on the formation of dental caries is still controversial. This study aimed to investigate the effect of two antibiotics (amoxicillin and potassium clavulanate suspension), with and without sucrose, on human dental hardness and Streptococcus mutans counts in dental biofilm. Primary tooth fragments (n=72) were coated with nail varnish leaving a window of 2.25 mm diameter. Specimens were fixed in 24-well polystyrene plates, containing BHI medium. S. mutans (clinical strains) represented the inoculum to form biofilm on the fragments for 24 h. Twelve fragments were separated for the initial count of microorganisms (baseline). The other fragments were divided into 4 groups (n=12) of treatment: G1 (Clavulin(r)), G2 (Betamox(r)), G3 (chlorhexidine 0.12%), G4 (sucrose 10%). All specimens had their self-control area (covered area). The cross-sectional microhardness (CSMH) was evaluated for each specimen. All the treated groups had a loss of hardness compared to their self-controls (p<0.05). Both drugs inhibited the S. mutans growth and promoted no CSMH difference among them. Both antibiotics eliminated all formed biofilm and did not cause mineral loss from the enamel, regardless the presence of sucrose in its formulation.
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Daniela Novaes
authors.initials: DN
authors.lastname: Soares
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Antonio
authors.affiliation: Department of Basic Science, Universidade Federal Fluminense, Nova Friburgo, RJ, Brasil.
authors.forename: Natalia Lopes Pontes
authors.initials: NL
authors.lastname: Iorio
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Viviane Santos da Silva
authors.initials: VS
authors.lastname: Pierro
authors.affiliation: Department of Medical Microbiology, Prof. Paulo de Góes Microbiology Institute, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Katia Regina Netto
authors.initials: KR
authors.lastname: dos Santos
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Lucianne Cople
authors.initials: LC
authors.lastname: Maia
mesh_terms: D018441:Biofilms; D002648:Child; D015169:Colony Count, Microbial; D003743:Dental Enamel / Q000187:drug effects*; D006801:Humans; D008903:Minerals / Q000378:metabolism*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000302:isolation & purification; D013395:Sucrose / Q000494:pharmacology*
chemical_list: D008903:Minerals; D013395:Sucrose