title: Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry.
abstract: Fibronectin fibrillogenesis is the physiological process by which cells elaborate a fibrous FN matrix. Poly(ethyl acrylate), PEA, has been described to induce a similar process upon simple adsorption of fibronectin (FN) from a protein solution-in the absence of cells-leading to the so-called material-driven fibronectin fibrillogenesis. Poly(methyl acrylate), PMA, is a polymer with very similar chemistry to PEA, on which FN is adsorbed, keeping the globular conformation of the protein in solution. We have used radical polymerization to synthesize copolymers with controlled EA/MA ratio, seeking to modulate the degree of FN fibrillogenesis. The physicochemical properties of the system were studied using dynamic-mechanical analysis, differential scanning calorimetry, and water contact angle. Both the degree of FN fibrillogenesis and the availability of the integrin binding region of FN directly depend on the percentage of EA in the copolymer, whereas the same total amount of FN was adsorbed regardless the EA/MA ratio. Cell morphology adhesion and differentiation of murine C2C12 were shown to depend on the degree of FN fibrillogenesis previously attained on the material surface. Myogenic differentiation was enhanced on the copolymers with higher EA content, i.e. more interconnected FN fibrils.
authors.affiliation: †Center for Biomaterials and Tissue Engineering. Universitat Politècnica de València, 46022 València, Spain.
authors.forename: Hayk
authors.initials: H
authors.lastname: Mnatsakanyan
authors.affiliation: †Center for Biomaterials and Tissue Engineering. Universitat Politècnica de València, 46022 València, Spain.|‡CIBER en Bioingeniería, Biomateriales y Nanomedicina, 46022 València, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Rico
authors.affiliation: §Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
authors.forename: Eleni
authors.initials: E
authors.lastname: Grigoriou
authors.affiliation: †Center for Biomaterials and Tissue Engineering. Universitat Politècnica de València, 46022 València, Spain.
authors.forename: Aarón Maturana
authors.initials: AM
authors.lastname: Candelas
authors.affiliation: §Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
authors.forename: Aleixandre
authors.initials: A
authors.lastname: Rodrigo-Navarro
authors.affiliation: §Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
authors.forename: Manuel
authors.initials: M
authors.lastname: Salmeron-Sanchez
authors.affiliation: †Center for Biomaterials and Tissue Engineering. Universitat Politècnica de València, 46022 València, Spain.
authors.forename: Roser
authors.initials: R
authors.lastname: Sabater i Serra
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D005353:Fibronectins; D011108:Polymers; C478961:poly(ethylacrylate); D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: bioactive substrates; biointerface; cell adhesion; cell differentiation; fibrillogenesis; fibronectin