title: Cytotoxicity of Fast-set Conventional and Resin-modified Glass Ionomer Cement Polymerized at Different Times on SHED.
abstract: OBJECTIVE
  To compare the cytotoxicity of conventional GIC and Resin Modified GIC (RMGIC) polymerized at 2 different times on stem cells from human exfoliated deciduous teeth (SHED).
  
  
  METHODS
  The conventional GIC (Fuji IX GP Extra) and RMGIC (Fuji II LC) were mixed and incubated in a prepared Dublecco's Modified Eagle Medium (DMEM) for seven days. After seeding the characterized SHED for 24 hrs, six replicates of seven serially diluted extracts of each group were added and incubated for 72 hrs. MTT test was used for cytotoxicity evaluation and the data were analysed using Kruskal-Wallis followed by Mann-Whitney test, with the statistical significance set at P<0.05.
  
  
  RESULTS
  The half maximal inhibitory concentration (IC50) was found at 45.0 mg/ml, 45.0 mg/ml and 31.25 mg/ml for Fuji IX, Fuji II LC (40s) and Fuji II LC (20s), respectively. Significantly different cytotoxic effects were found between Fuji II LC polymerized at 20 secs and 40 secs, and between Fuji IX and Fuji II LC (20s) (P<0.05), and these were observed in all concentrations except for 50 mg/ml.
  
  
  CONCLUSIONS
  RMGIC polymerized at 20 secs exhibited the least favorable cell viability among all groups. Nevertheless, the cell viability was comparable to conventional GICs when the manufacturer's recommended time was doubled (40 secs).
authors.affiliation: Department of Restorative Dentistry, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Roszeyana
authors.initials: R
authors.lastname: Mohd Zainal Abidin
authors.affiliation: Department of Restorative Dentistry, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Norhayati
authors.initials: N
authors.lastname: Luddin
authors.affiliation: Department of Restorative Dentistry, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Nor
authors.initials: N
authors.lastname: Shamsuria Omar
authors.affiliation: Department of Restorative Dentistry, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Hany
authors.initials: H
authors.lastname: Mohamed Aly Ahmed
mesh_terms: D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D005899:Glass Ionomer Cements / Q000633:toxicity*; D006801:Humans; D008422:Materials Testing; D058105:Polymerization; D019279:Resin Cements / Q000633:toxicity*; D012117:Resins, Synthetic / Q000633:toxicity; D013234:Stem Cells / Q000187:drug effects*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014094:Tooth, Deciduous / Q000166:cytology* / Q000187:drug effects
chemical_list: D004396:Coloring Agents; C079765:Fuji II LC cement; C439808:Fuji IX GP Fast; D005899:Glass Ionomer Cements; D019279:Resin Cements; D012117:Resins, Synthetic; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue
keywords: Glass ionomer cement; SHED; cytotoxicity; polymerization time; resin modified glass ionomer cement