title: Anti-NeuGcGM3 reactivity: a possible role of natural antibodies and B-1 cells in tumor immunosurveillance.
abstract: While not naturally expressed in normal human tissues, N-glycolylated (NeuGc) gangliosides are overexpressed in several tumors and have immunosuppressive capacity, which contributes to cancer progression. Naturally occurring antibodies against NeuGcGM3 exist in healthy donors that specifically recognize and kill tumor cells expressing the antigen by complement-dependent and -independent mechanisms, the latter resembling an oncotic necrosis-type of cell death. Both the levels of anti-NeuGcGM3 antibodies in the sera of healthy donors and the percentage of donors with these natural antibodies decrease with age. Our work has shown that anti-NeuGcGM3 antibodies are not detected in the sera of non-small cell lung cancer (NSCLC) patients, compared to age- and sex-matched healthy donors, which have anti-NeuGcGM3. Interestingly, the level of serum total IgM, but not IgG, was significantly lower in cancer patients than in healthy donors. Screening of immortalized mouse splenic and peritoneal-derived hybridomas showed that peritoneal B-1 cells secrete anti-NeuGcGM3 with tumor cytotoxic capacity. Defects in the natural surveillance against tumor antigens could increase the risk of elderly donors developing cancer and affect the capacity of cancer patients to effectively fight against tumor cells.
authors.affiliation: Natural Antibodies Group, Tumor Immunology Division, Center of Molecular Immunology, Havana, Cuba.
authors.forename: Nely
authors.initials: N
authors.lastname: Rodriguez-Zhurbenko
authors.affiliation: Chimeric Proteins Group, Immunobiology Division, Center of Molecular Immunology, Havana, Cuba.
authors.forename: Maura
authors.initials: M
authors.lastname: Rabade-Chediak
authors.affiliation: Natural Antibodies Group, Tumor Immunology Division, Center of Molecular Immunology, Havana, Cuba.
authors.forename: Darel
authors.initials: D
authors.lastname: Martinez
authors.affiliation: Natural Antibodies Group, Tumor Immunology Division, Center of Molecular Immunology, Havana, Cuba.
authors.forename: Tania
authors.initials: T
authors.lastname: Griñan
authors.affiliation: Natural Antibodies Group, Tumor Immunology Division, Center of Molecular Immunology, Havana, Cuba.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Hernandez
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D004285:Dogs; D005260:Female; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D006736:Horses; D006801:Humans; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007157:Immunologic Surveillance / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D001323:Autoantibodies; D005679:G(M3) Ganglioside; C044298:N-glycolylneuraminyllactosylceramide
keywords: NeuGcGM3 ganglioside; aging; cancer; natural antibodies