title: Optimization of influenza A vaccine virus by reverse genetic using chimeric HA and NA genes with an extended PR8 backbone.
abstract: The yield of influenza antigen production may significantly vary between vaccine strains; for example the A/California/07/09 (H1N1)-X179A vaccine virus, prepared during 2009 influenza pandemic, presented a low antigen yield in eggs compared to other seasonal H1N1 reassortants. In this study a bi-chimeric virus expressing HA and NA genes with A/Puerto Rico/8/34 (H1N1) (PR8) and X179A domains was rescued by reverse genetics using a mixture of Vero/CHOK1 cell lines (Medina et al. [7]). The bi-chimeric virus obtained demonstrated to yield much larger amounts of HA than X179A in eggs as measured by single-radial-immunodiffusion (SRID), the reference method to quantify HA protein in influenza vaccine. Such kind of optimized virus using PR8 backbone derived chimeric glycoproteins could be used as improved seed viruses for vaccine production.
authors.affiliation: Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux, 69280 Marcy L'Etoile, France. Electronic address: julie.medina@live.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Medina
authors.affiliation: Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux, 69280 Marcy L'Etoile, France. Electronic address: Houda.Boukhebza@sanofipasteur.com.
authors.forename: Houda
authors.initials: H
authors.lastname: Boukhebza
authors.affiliation: Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux, 69280 Marcy L'Etoile, France. Electronic address: amelie.desaintjean@free.fr.
authors.forename: Amélie
authors.initials: A
authors.lastname: De Saint Jean
authors.affiliation: Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux, 69280 Marcy L'Etoile, France. Electronic address: regis.sodoyer@bioaster.org.
authors.forename: Régis
authors.initials: R
authors.lastname: Sodoyer
authors.affiliation: Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux, 69280 Marcy L'Etoile, France. Electronic address: isabelle.legastelois@sanofipasteur.com.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Legastelois
authors.affiliation: Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux, 69280 Marcy L'Etoile, France. Electronic address: catherine.moste@sanofipasteur.com.
authors.forename: Catherine
authors.initials: C
authors.lastname: Moste
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002522:Chlorocebus aethiops; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D007252:Influenza Vaccines / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D061985:Madin Darby Canine Kidney Cells; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D016865:Reassortant Viruses / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D059386:Reverse Genetics / Q000379:methods*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014709:Vero Cells
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011993:Recombinant Fusion Proteins; D009439:Neuraminidase
keywords: Chimeric; Influenza; PR8 backbone; Vaccine