title: MicroRNA-320a acts as a tumor suppressor by targeting BCR/ABL oncogene in chronic myeloid leukemia.
abstract: Accumulating evidences demonstrated that the induction of epithelial-mesenchymal transition (EMT) and aberrant expression of microRNAs (miRNAs) are associated with tumorigenesis, tumor progression, metastasis and relapse in cancers, including chronic myeloid leukemia (CML). We found that miR-320a expression was reduced in K562 and in CML cancer stem cells. Moreover, we found that miR-320a inhibited K562 cell migration, invasion, proliferation and promoted apoptosis by targeting BCR/ABL oncogene. As an upstream regulator of BCR/ABL, miR-320a directly targets BCR/ABL. The enhanced expression of miR-320a inhibited the phosphorylation of PI3K, AKT and NF-κB; however, the expression of phosphorylated PI3K, AKT and NF-κB were restored by the overexpression of BCR/ABL. In K562, infected with miR-320a or transfected with SiBCR/ABL, the protein levels of fibronectin, vimentin, and N-cadherin were decreased, but the expression of E-cadherin was increased. The expression of mesenchymal markers in miR-320a-expressing cells was restored to normal levels by the restoration of BCR/ABL expression. Generally speaking, miR-320a acts as a novel tumor suppressor gene in CML and miR-320a can decrease migratory, invasive, proliferative and apoptotic behaviors, as well as CML EMT, by attenuating the expression of BCR/ABL oncogene.
authors.affiliation: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, 0086-027-7398722, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Xishan
authors.affiliation: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, 0086-027-7398722, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ziying
authors.affiliation: Weifang Traditional Chinese Medicine Hospital, Department of Urology. 0086-0536-8300338, China.
authors.forename: Du
authors.initials: D
authors.lastname: Jing
authors.affiliation: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, 0086-027-7398722, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Gang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D016147:Genes, Tumor Suppressor; D006801:Humans; D020014:K562 Cells / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology / Q000502:physiology; D023041:Xenograft Model Antitumor Assays; D055815:Young Adult
chemical_list: C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D016044:Fusion Proteins, bcr-abl