title: Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Downregulates ELOVL1 Gene Expression and Fatty Acid Synthesis in Goat Fetal Fibroblasts.
abstract: Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene that belongs to the ELOVL family and regulates the synthesis of very-long-chain fatty acids (VLCFAs) and sphingolipids, from yeast to mammals. Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell metabolism and is associated with fatty acids synthesis. In this study, we cloned the cDNA that encodes Cashmere goat (Capra hircus) ELOVL1 (GenBank Accession number KF549985) and investigated its expression in 10 tissues. ELOVL1 cDNA was 840 bp, encoding a deduced protein of 279 amino acids, and ELOVL1 mRNA was expressed in a wide range of tissues. Inhibition of mTORC1 by rapamycin decreased ELOVL1 expression and fatty acids synthesis in Cashmere goat fetal fibroblasts. These data show that ELOVL1 expression is regulated by mTORC1 and that mTORC1 has significant function in fatty acids synthesis in Cashmere goat.
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. quintas@live.cn.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. qbr2010@126.com.
authors.forename: Qiburi
authors.initials: Q
authors.lastname: He
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. GZX770461760@163.com.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. yangliminlh@163.com.
authors.forename: Limin
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. baolili1203@126.com.|College of Basic Medical Science, Inner Mongolia Medical University, Hohhot 010110, China. baolili1203@126.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Bao
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. bwlnhm@sina.com.
authors.forename: Wenlei
authors.initials: W
authors.lastname: Bao
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. dong2186@163.com.|Hulunbeir Municipal People's Hospital, Hailar 021008, China. dong2186@163.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. wangyf@life.imu.edu.cn.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot 010021, China. lswzg@imu.edu.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015536:Down-Regulation; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D008297:Male; D000076222:Mechanistic Target of Rapamycin Complex 1; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000037:antagonists & inhibitors*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors*
chemical_list: D005227:Fatty Acids; D046912:Multiprotein Complexes; D012333:RNA, Messenger; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases
keywords: ELOVL1; cashmere goat; expression pattern