title: In house validation from direct determination of 5-hydroxymethyl-2-furfural (HMF) in Brazilian corn and cane syrups samples by HPLC-UV.
abstract: In this work the development and in house validation of the HMF direct determination in corn and cane syrups by HPLC-UV was carried out for the first time. The separation was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v/v) on Phenomenex C18 column (5.0 μm, 4.6 × 150 mm), at 30 °C, flow rate of 0.8 mL min(-1) and detection at 285 nm. The validated method showed excellent performance with low limits (LOD and LOQ of 0.09 and 0.26 mg L(-1), respectively), good accuracy (recovery rates between 100% and 104%) and precision (RSD's for repeatability and intermediate precision between 0.57% and 6.43%). Good selectivity and linearity were also observed. HMF contents in both foods were very high (406.6-2121.3 mg kg(-1) for corn syrup and 109.2-893.1 mg kg(-1) for cane syrup), which arouses concern about food safety of these products.
authors.affiliation: Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil. Electronic address: jucimarakulekdeandrade@gmail.com.
authors.forename: Jucimara K
authors.initials: JK
authors.lastname: de Andrade
authors.affiliation: Chemistry Department, University of Manitoba, 144 Dysart Road, R3T 2N2 Winnipeg, MB, Canada.
authors.forename: Emy
authors.initials: E
authors.lastname: Komatsu
authors.affiliation: Chemistry Department, University of Manitoba, 144 Dysart Road, R3T 2N2 Winnipeg, MB, Canada.
authors.forename: Hélène
authors.initials: H
authors.lastname: Perreault
authors.affiliation: Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
authors.forename: Yohandra R
authors.initials: YR
authors.lastname: Torres
authors.affiliation: Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: da Rosa
authors.affiliation: Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Felsner
mesh_terms: D001938:Brazil; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde
keywords: 5-Hydroxymethyl-2-furfural (HMF); Cane syrups; Corn syrups; HPLC–UV; In house validation