title: DNA nanopore translocation in glutamate solutions.
abstract: Nanopore experiments have traditionally been carried out with chloride-based solutions. Here we introduce silver/silver-glutamate-based electrochemistry as an alternative, and study the viscosity, conductivity, and nanopore translocation characteristics of potassium-, sodium-, and lithium-glutamate solutions. We show that it has a linear response at typical voltages and can be used to detect DNA translocations through a nanopore. The glutamate anion also acts as a redox-capable thickening agent, with high-viscosity solutions capable of slowing down the DNA translocation process by up to 11 times, with a corresponding 7 time reduction in signal. These results demonstrate that glutamate can replace chloride as the primary anion in nanopore resistive pulse sensing.
authors.affiliation: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands. c.dekker@tudelft.nl.
authors.forename: C
authors.initials: C
authors.lastname: Plesa
authors.forename: N
authors.initials: N
authors.lastname: van Loo
authors.forename: C
authors.initials: C
authors.lastname: Dekker
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004553:Electric Conductivity; D055664:Electrochemical Techniques; D004566:Electrodes; D004867:Equipment Design; D018698:Glutamic Acid / Q000737:chemistry* / Q000378:metabolism*; D058608:Nanopores; D036103:Nanotechnology; D011189:Potassium Chloride / Q000737:chemistry; D014783:Viscosity
chemical_list: D018698:Glutamic Acid; D011189:Potassium Chloride; D004247:DNA