title: Anions in Nucleic Acid Crystallography.
abstract: Nucleic acid crystallization buffers contain a large variety of chemicals fitting specific needs. Among them, anions are often solely considered for pH-regulating purposes and as cationic co-salts while their ability to directly bind to nucleic acid structures is rarely taken into account. Here we review current knowledge related to the use of anions in crystallization buffers along with data on their biological prevalence. Chloride ions are frequently identified in crystal structures but display low cytosolic concentrations. Hence, they are thought to be distant from nucleic acid structures in the cell. Sulfate ions are also frequently identified in crystal structures but their localization in the cell remains elusive. Nevertheless, the characterization of the binding properties of these ions is essential for better interpreting the solvent structure in crystals and consequently, avoiding mislabeling of electron densities. Furthermore, understanding the binding properties of these anions should help to get clues related to their potential effects in crowded cellular environments.
authors.affiliation: Architecture et Réactivité de l'ARN, Institut de Biologie Moléculaire et Cellulaire du CNRS, UPR 9002 CNRS/Université de Strasbourg, 15, rue René Descartes, Strasbourg Cedex, 67084, France.
authors.forename: Luigi
authors.initials: L
authors.lastname: D'Ascenzo
authors.forename: Pascal
authors.initials: P
authors.lastname: Auffinger
mesh_terms: D000838:Anions; D001665:Binding Sites; D002021:Buffers; D002712:Chlorides / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D003600:Cytosol / Q000378:metabolism; D057225:Data Mining; D030562:Databases, Protein; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008024:Ligands; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D012997:Solvents / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry
chemical_list: D000838:Anions; D002021:Buffers; D002712:Chlorides; D007477:Ions; D008024:Ligands; D009696:Nucleic Acids; D010710:Phosphates; D012997:Solvents; D013431:Sulfates