title: Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics.
abstract: Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution.
authors.affiliation: German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg, Germany.|Bioquant Center, Heidelberg, Germany.
authors.forename: Jale
authors.initials: J
authors.lastname: Schneider
authors.affiliation: German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg, Germany.|Bioquant Center, Heidelberg, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Zahn
authors.affiliation: Institute for Biology and Genetics, Free University of Berlin, Berlin, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Maglione
authors.affiliation: Institute for Biology and Genetics, Free University of Berlin, Berlin, Germany.
authors.forename: Stephan J
authors.initials: SJ
authors.lastname: Sigrist
authors.affiliation: German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg, Germany.|Bioquant Center, Heidelberg, Germany.
authors.forename: Jonas
authors.initials: J
authors.lastname: Marquard
authors.affiliation: Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Jakub
authors.initials: J
authors.lastname: Chojnacki
authors.affiliation: Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Kräusslich
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Steffen J
authors.initials: SJ
authors.lastname: Sahl
authors.affiliation: German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg, Germany.|Bioquant Center, Heidelberg, Germany.
authors.forename: Johann
authors.initials: J
authors.lastname: Engelhardt
authors.affiliation: German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Heidelberg, Germany.|Bioquant Center, Heidelberg, Germany.|Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D003627:Data Interpretation, Statistical; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D010783:Photometry / Q000295:instrumentation*; D013269:Stochastic Processes