title: In Vivo Integrity and Biological Fate of Chelator-Free Zirconium-89-Labeled Mesoporous Silica Nanoparticles.
abstract: Traditional chelator-based radio-labeled nanoparticles and positron emission tomography (PET) imaging are playing vital roles in the field of nano-oncology. However, their long-term in vivo integrity and potential mismatch of the biodistribution patterns between nanoparticles and radio-isotopes are two major concerns for this approach. Here, we present a chelator-free zirconium-89 ((89)Zr, t1/2 = 78.4 h) labeling of mesoporous silica nanoparticle (MSN) with significantly enhanced in vivo long-term (>20 days) stability. Successful radio-labeling and in vivo stability are demonstrated to be highly dependent on both the concentration and location of deprotonated silanol groups (-Si-O(-)) from two types of silica nanoparticles investigated. This work reports (89)Zr-labeled MSN with a detailed labeling mechanism investigation and long-term stability study. With its attractive radio-stability and the simplicity of chelator-free radio-labeling, (89)Zr-MSN offers a novel, simple, and accurate way for studying the in vivo long-term fate and PET image-guided drug delivery of MSN in the near future.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Shreya
authors.initials: S
authors.lastname: Goel
authors.forename: Hector F
authors.initials: HF
authors.lastname: Valdovinos
authors.forename: Haiming
authors.initials: H
authors.lastname: Luo
authors.forename: Reinier
authors.initials: R
authors.lastname: Hernandez
authors.forename: Todd E
authors.initials: TE
authors.lastname: Barnhart
authors.affiliation: University of Wisconsin Carbone Cancer Center , Madison, Wisconsin 53705, United States.
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D007275:Injections, Intravenous; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use* / Q000648:ultrastructure; D016062:Porosity; D049268:Positron-Emission Tomography / Q000379:methods*; D011868:Radioisotopes / Q000493:pharmacokinetics* / Q000627:therapeutic use; D012822:Silicon Dioxide / Q000737:chemistry / Q000493:pharmacokinetics*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D015040:Zirconium / Q000493:pharmacokinetics* / Q000627:therapeutic use
chemical_list: D011868:Radioisotopes; D012822:Silicon Dioxide; D015040:Zirconium
keywords: chelator-free radio-labeling; mesoporous silica nanoparticle; positron emission tomography; zirconium-89