title: Interferometric Reflectance Imaging Sensor (IRIS)--A Platform Technology for Multiplexed Diagnostics and Digital Detection.
abstract: Over the last decade, the growing need in disease diagnostics has stimulated rapid development of new technologies with unprecedented capabilities. Recent emerging infectious diseases and epidemics have revealed the shortcomings of existing diagnostics tools, and the necessity for further improvements. Optical biosensors can lay the foundations for future generation diagnostics by providing means to detect biomarkers in a highly sensitive, specific, quantitative and multiplexed fashion. Here, we review an optical sensing technology, Interferometric Reflectance Imaging Sensor (IRIS), and the relevant features of this multifunctional platform for quantitative, label-free and dynamic detection. We discuss two distinct modalities for IRIS: (i) low-magnification (ensemble biomolecular mass measurements) and (ii) high-magnification (digital detection of individual nanoparticles) along with their applications, including label-free detection of multiplexed protein chips, measurement of single nucleotide polymorphism, quantification of transcription factor DNA binding, and high sensitivity digital sensing and characterization of nanoparticles and viruses.
authors.affiliation: Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's Street, Boston, MA 02215, USA. oguzhan@bu.edu.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Avci
authors.affiliation: Department of Biomedical Engineering, Boston University, 8 St. Mary's Street, Boston, MA 02215, USA. aozkumur@gmail.com.|Department of Electrical and Electronics Engineering and School of Medicine, Bahcesehir University, Ciragan Caddesi, No:4-6, Besiktas, Istanbul 34353, Turkey. aozkumur@gmail.com.
authors.forename: Nese Lortlar
authors.initials: NL
authors.lastname: Ünlü
authors.affiliation: Department of Electrical and Electronics Engineering and School of Medicine, Bahcesehir University, Ciragan Caddesi, No:4-6, Besiktas, Istanbul 34353, Turkey. aozkumur@gmail.com.
authors.forename: Ayça Yalçın
authors.initials: AY
authors.lastname: Özkumur
authors.affiliation: Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's Street, Boston, MA 02215, USA. selim@bu.edu.|Department of Biomedical Engineering, Boston University, 8 St. Mary's Street, Boston, MA 02215, USA. selim@bu.edu.
authors.forename: M Selim
authors.initials: MS
authors.lastname: Ünlü
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003952:Diagnostic Imaging / Q000295:instrumentation*; D006801:Humans; D007368:Interferometry / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted
keywords: digital detection; interferometry; label-free; nanoparticle; optical biosensor; single nucleotide polymorphism; virus