title: Universal Fluorescence Biosensor Platform Based on Graphene Quantum Dots and Pyrene-Functionalized Molecular Beacons for Detection of MicroRNAs.
abstract: A novel biosensor platform was developed for detection of microRNAs (miRNAs) based on graphene quantum dots (GQDs) and pyrene-functionalized molecular beacon probes (py-MBs). Pyrene was introduced to trigger specifically fluorescence resonance energy transfer (FRET) between GQDs and fluorescent dyes labeled on py-MBs, and the unique fluorescent intensity change produced a novel signal for detection of the target. The platform realized detection of miRNAs in a wide range from 0.1 nM to 200 nM with great discrimination abilities, as well as multidetection of different kinds of miRNAs, which paved a brand new way for miRNA detection based on GQDs.
authors.affiliation: ‡Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: §Thyroid Surgery, Second Affiliated Hospital of Guizhou Medical University, Kaili 556000, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: §Thyroid Surgery, Second Affiliated Hospital of Guizhou Medical University, Kaili 556000, China.
authors.forename: Daiwei
authors.initials: D
authors.lastname: Zhao
authors.affiliation: ‡Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zeng
authors.affiliation: ⊥School of Chemistry and Biology Engineering, Changsha University of Science and Technology, Changsha 410114, China.|∥Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Jiaoyun
authors.initials: J
authors.lastname: Xia
authors.affiliation: #Chemistry Department, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Aldalbahi
authors.affiliation: ‡Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Wang
authors.affiliation: ‡Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
authors.forename: Lili
authors.initials: L
authors.lastname: San
authors.affiliation: ∥Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: ∥Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: ‡Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
authors.forename: Xianqiang
authors.initials: X
authors.lastname: Mi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D015336:Molecular Probe Techniques / Q000295:instrumentation; D015335:Molecular Probes / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D035683:MicroRNAs; D015335:Molecular Probes; D011721:Pyrenes; D006108:Graphite; C030984:pyrene
keywords: biosensor; fluorescence resonance energy transfer; graphene quantum dots; microRNA; pyrene