title: Regulation of endomembrane biogenesis in arabidopsis by phospatidic acid hydrolase.
abstract: Coordination of membrane lipid biosynthesis is important for cell function during plant growth and development. Here we summarize our recent work on PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) which suggests that this enzyme is a key regulator of phosphaticylcholine (PC) biosynthesis in Arabidopsis thaliana. Disruption of PAH activity elevates phosphatidic acid (PA) levels and stimulates PC biosynthesis and biogenesis of the endoplasmic reticulum (ER). Furthermore, the activity of PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE (CCT), which is the key enzyme controlling the rate of PC biosynthesis, is directly stimulated by PA and expression of a constitutively active version of CCT replicates the effects of PAH disruption. Hence PAH activity can control the abundance of PA, which in turn can modulate CCT activity to govern the rate of PC biosynthesis. Crucially it is not yet clear how PAH activity is regulated in Arabidopsis but there is evidence that PAH1 and PAH2 are both phosphorylated and further work will be required to investigate whether this is functionally significant.
authors.affiliation: a Department of Plant Biology and Crop Science ; Rothamsted Research, Harpenden ; Hertfordshire , UK.
authors.forename: Christian P
authors.initials: CP
authors.lastname: Craddock
authors.affiliation: a Department of Plant Biology and Crop Science ; Rothamsted Research, Harpenden ; Hertfordshire , UK.
authors.forename: Nicolette
authors.initials: N
authors.lastname: Adams
authors.affiliation: a Department of Plant Biology and Crop Science ; Rothamsted Research, Harpenden ; Hertfordshire , UK.
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Bryant
authors.affiliation: a Department of Plant Biology and Crop Science ; Rothamsted Research, Harpenden ; Hertfordshire , UK.
authors.forename: Smita
authors.initials: S
authors.lastname: Kurup
authors.affiliation: a Department of Plant Biology and Crop Science ; Rothamsted Research, Harpenden ; Hertfordshire , UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Eastmond
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism; D008563:Membrane Lipids / Q000096:biosynthesis; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D029681:Arabidopsis Proteins; D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D019916:Choline-Phosphate Cytidylyltransferase; D006867:Hydrolases; D010711:Phosphatidate Phosphatase
keywords: Arabidopsis; cell cycle; membrane biogenesis; phosphatidic acid phosphohydrolase; phosphatidylcholine biosynthesis