title: In vivo comet assay of acrylonitrile, 9-aminoacridine hydrochloride monohydrate and ethanol in rats.
abstract: As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay, we examined the ability of acrylonitrile, 9-aminoacridine hydrochloride monohydrate (9-AA), and ethanol to induce DNA damage in the liver and glandular stomach of male rats. Acrylonitrile is a genotoxic carcinogen, 9-AA is a genotoxic non-carcinogen, and ethanol is a non-genotoxic carcinogen. Positive results were obtained in the liver cells of male rats treated with known genotoxic compounds, acrylonitrile and 9-AA.
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan. Electronic address: nakagawa.y@fdsc.or.jp.
authors.forename: Yuzuki
authors.initials: Y
authors.lastname: Nakagawa
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Toyoizumi
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Sui
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ohta
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Kumagai
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Usumi
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, Hadano, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamakage
mesh_terms: D000181:Acrylonitrile / Q000633:toxicity*; D000585:Aminacrine / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D008099:Liver / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000187:drug effects
chemical_list: D002273:Carcinogens; D000431:Ethanol; D000585:Aminacrine; D000181:Acrylonitrile
keywords: 9-Aminoacridine hydrochloride monohydrate; Acrylonitrile; Ethanol; In vivo rat comet assay; JaCVAM validation study