title: Trypanosoma cruzi population dynamics in the Central Ecuadorian Coast.
abstract: Chagas disease is the most important parasitic disease in Latin America. The causative agent, Trypanosoma cruzi, displays high genetic diversity and circulates in complex transmission cycles among domestic, peridomestic and sylvatic environments. In Ecuador, Rhodnius ecuadoriensis is known to be the major vector species implicated in T. cruzi transmission. However, across vast areas of Ecuador, little is known about T. cruzi genetic diversity in relation to different parasite transmission scenarios. Fifty-eight T. cruzi stocks from the central Ecuadorian coast, most of them derived from R. ecuadoriensis, were included in the study. All of them were genotyped as T. cruzi discrete typing unit I (DTU TcI). Analysis of 23 polymorphic microsatellite loci through neighbor joining and discriminant analysis of principal components yielded broadly congruent results and indicate genetic subdivision between sylvatic and peridomestic transmission cycles. However, both analyses also suggest that any barriers are imperfect and significant gene flow between parasite subpopulations in different habitats exists. Also consistent with moderate partition and residual gene flow between subpopulations, the fixation index (FST) was significant, but of low magnitude. Finally, the lack of private alleles in the domestic/peridomestic transmission cycle suggests the sylvatic strains constitute the ancestral population. The T. cruzi population in the central Ecuadorian coast shows moderate tendency to subdivision according to transmission cycle. However, connectivity between cycles exists and the sylvatic T. cruzi population harbored by R. ecuadoriensis vectors appears to constitute a source from which the parasite invades human domiciles and their surroundings in this region. We discuss the implications these findings have for the planning, implementation and evaluation of local Chagas disease control interventions.
authors.affiliation: Center for Infectious and Chronic Disease Research, School of Biological Sciences, Pontifical Catholic University of Ecuador, Quito, Ecuador; Tropical Disease Institute, Biomedical Sciences Department, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH, USA.
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Costales
authors.affiliation: Carrera de Ingeniería en Biotecnología, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas - ESPE, Sangolquí, Ecuador.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Jara-Palacios
authors.affiliation: Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, University of Wales, Bangor, Gwynedd, United Kingdom.
authors.forename: Martin S
authors.initials: MS
authors.lastname: Llewellyn
authors.affiliation: Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom.
authors.forename: Louisa A
authors.initials: LA
authors.lastname: Messenger
authors.affiliation: Center for Infectious and Chronic Disease Research, School of Biological Sciences, Pontifical Catholic University of Ecuador, Quito, Ecuador; Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, University of Wales, Bangor, Gwynedd, United Kingdom.
authors.forename: Sofía
authors.initials: S
authors.lastname: Ocaña-Mayorga
authors.affiliation: Center for Infectious and Chronic Disease Research, School of Biological Sciences, Pontifical Catholic University of Ecuador, Quito, Ecuador.
authors.forename: Anita G
authors.initials: AG
authors.lastname: Villacís
authors.affiliation: Maladies Infectieuses et Vecteurs Ecologie, Génétique, Evolution et Contrôle, MIVEGEC (IRD 224-CNRS 5290-UM1-UM2), IRD Center, Montpellier, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Tibayrenc
authors.affiliation: Center for Infectious and Chronic Disease Research, School of Biological Sciences, Pontifical Catholic University of Ecuador, Quito, Ecuador; Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, University of Wales, Bangor, Gwynedd, United Kingdom. Electronic address: grijalva@ohio.edu.
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Grijalva
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D000835:Animals, Wild / Q000469:parasitology*; D014355:Chagas Disease / Q000453:epidemiology* / Q000469:parasitology*; D017753:Ecosystem; D004484:Ecuador / Q000453:epidemiology; D051456:Gene Flow; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D011157:Population Dynamics; D012239:Rhodnius / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000235:genetics*
keywords: Chagas disease; DAPC; Ecuador; Rhodnius ecuadoriensis; Transmission cycle; Trypanosoma cruzi; microsatellite