title: Butanol production by a Clostridium beijerinckii mutant with high ferulic acid tolerance.
abstract: A mutant strain of Clostridium beijerinckii, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high-throughput screening of C. beijerinckii NCIMB 8052. The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid, which is comparable to the production from non-ferulic acid cultures (8.11 g/L of butanol). When 0.8 g/L of ferulic acid was introduced into the P2 medium, C. beijerinckii M11 grew well and produced 4.91 g/L of butanol. Both cell growth and butanol production of C. beijerinckii M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium. Furthermore, C. beijerinckii M11 could produce 6.13 g/L of butanol using non-detoxified hemicellulosic hydrolysate from diluted sulfuric acid-treated corn fiber (SAHHC) as the carbon source. These results demonstrate that C. beijerinckii M11 has a high ferulic acid tolerance and is able to use non-detoxified SAHHC for butanol production.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.|National Engineering Technique Research Center for Biotechnology, Nanjing, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. guoting@hotmail.com.|National Engineering Technique Research Center for Biotechnology, Nanjing, People's Republic of China. guoting@hotmail.com.|Guangzhou Sugarcane Industry Research Institute, Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangdong Engineering Research & Development Center for Comprehensive Utilization of Plant Fiber, Guangzhou Key Laboratory for Comprehensive Utilization of Plant Fiber, Guangzhou, People's Republic of China. guoting@hotmail.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.|National Engineering Technique Research Center for Biotechnology, Nanjing, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.|National Engineering Technique Research Center for Biotechnology, Nanjing, People's Republic of China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. yinghanjie@njtech.edu.cn.|National Engineering Technique Research Center for Biotechnology, Nanjing, People's Republic of China. yinghanjie@njtech.edu.cn.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D001274:Atmospheric Pressure; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D003373:Coumaric Acids / Q000494:pharmacology*; D005285:Fermentation; D016296:Mutagenesis; D009154:Mutation; D013464:Sulfuric Acids / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
chemical_list: D000440:Butanols; D003373:Coumaric Acids; D013464:Sulfuric Acids; C004999:ferulic acid; C033158:sulfuric acid
keywords: Clostridium beijerinckii; fermentation; ferulic acid; high-throughput screening; non-detoxified hemicellulosic hydrolysate