title: Morphological and molecular genetic characterization of three Capillaria spp. (Capillaria anatis, Capillaria pudendotecta, and Capillaria madseni) and Baruscapillaria obsignata (Nematoda: Trichuridae: Capillariinae) in avians.
abstract: Morphological and genetic analyses were performed on four avian species of the subfamily Capillariinae (Nematoda: Trichuridae), i.e., Capillaria anatis from chickens (Gallus gallus domesticus) in Japan and the Philippines, Baruscapillaria obsignata from chickens and captive swans (Cygnus olor and Cygnus atratus) in Japan, Capillaria pudendotecta from captive swans in Japan, and Capillaria madseni from carrion and jungle crows (Corvus corone and Corvus macrorhynchos) in Japan. Although morphometric variations of male and female worms from different hosts and/or localities made the species identification difficult, the 18S ribosomal RNA gene (rDNA) sequences clarified their taxonomic position and phylogenetic relationships. Species of the same genus clustered robustly into a single clade in the phylogenetic tree based on the 18S rDNA, demonstrating to the extent possible the validity of the latest classification of the subfamily following Moravec's rearrangement in 1982. Male worms of C. pudendotecta are described here for the first time.
authors.affiliation: Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Tamaru
authors.affiliation: Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Yamaki
authors.affiliation: Davao Oriental State College of Science and Technology, 8200 Mati, Davao Oriental, Republic of the Philippines.
authors.forename: Lea Angsinco
authors.initials: LA
authors.lastname: Jimenez
authors.affiliation: Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. sato7dp4@yamaguchi-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
mesh_terms: D000818:Animals; D001483:Base Sequence; D002195:Capillaria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D002645:Chickens / Q000469:parasitology*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D009348:Nematoda / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D009349:Nematode Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: Baruscapillaria; Capillaria; Capillariinae; Molecular phylogeny; Morphological variation; Poultry; Taxonomy; rDNA