title: Microfluidic reflow pumps.
abstract: A new microfluidic pump, termed a reflow pump, is designed to operate with a sub-μl sample volume and transport it back and forth between two pneumatically actuated reservoirs through a flow channel typically containing one or more sensor surfaces. The ultimate motivation is to efficiently use the small sample volume in conjunction with convection to maximize analyte flux to the sensor surface(s) in order to minimize sensor response time. In this paper, we focus on the operational properties of the pumps themselves (rather than the sensor surfaces), and demonstrate both two-layer and three-layer polydimethylsiloxane reflow pumps. For the three-layer pump, we examine the effects of reservoir actuation pressure and actuation period, and demonstrate average volumetric flow rates as high as 500 μl/min. We also show that the two-layer design can pump up to 93% of the sample volume during each half period and demonstrate integration of a reflow pump with a single-chip microcantilever array to measure maximum flow rate.
authors.affiliation: Electrical and Computer Engineering Department, Brigham Young University , Provo, Utah 84602, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Haslam
authors.affiliation: Electrical and Computer Engineering Department, Brigham Young University , Provo, Utah 84602, USA.
authors.forename: Long-Fang
authors.initials: LF
authors.lastname: Tsai
authors.affiliation: Electrical and Computer Engineering Department, Brigham Young University , Provo, Utah 84602, USA.
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Anderson
authors.affiliation: Electrical and Computer Engineering Department, Brigham Young University , Provo, Utah 84602, USA.
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Electrical and Computer Engineering Department, Brigham Young University , Provo, Utah 84602, USA.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Hu
authors.affiliation: Electrical and Computer Engineering Department, Brigham Young University , Provo, Utah 84602, USA.
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Nordin