title: Effectiveness of hyperbaric oxygen for experimental treatment of schistosomiasis mansoni using praziquantel-free and encapsulated into liposomes: assay in adult worms and oviposition.
abstract: The treatment of schistosomiasis depends on a single drug: praziquantel (PZQ). However, this treatment presents limitations such as low and/or erratic bioavailability that can contribute to cases of tolerance. Improvements to the available drug are urgently needed and studies with a controlled system of drug release, like liposomes, have been gaining prominence. The present study evaluated the activity and synergy between liposomal-praziquantel (lip.PZQ) and hyperbaric oxygen therapy (HBO). Mice received doses of 60 or 100mg/kg PZQ or lip.PZQ, 50 days post-infection, and after the treatment, were exposed to HBO (3 atmosphere absolute - ATA) for 1h. The viability of adult worms and oviposition were analyzed, by necropsy and Kato-Katz examination performed after 15 days of treatment. A concentration of 100mg/kg of lip.PZQ+HBO was more effective (48.0% reduction of worms, 83.3% reduction of eggs/gram of feces) and 100% of the mice had altered of oograms (indicating interruption of oviposition) compared to other treatments and to the Control group (infected and untreated). It is known that PZQ requires participation of the host immune system to complete its antischistosomal activity and that HBO is able to stimulate the immune system. The drug became more available in the body when incorporated into liposomes and, used with HBO, the HBO worked as an adjuvant. This explains the decreases of oviposition and worms recovered form hepatic portal system.
authors.affiliation: Departamento de Biologia Animal, Laboratório de Helmintologia, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil; Instituto Federal de Educação Ciência e Tecnologia de São Paulo, Licenciatura em Ciências Biológicas, IFSP, Avaré, SP, Brazil. Electronic address: taferraz@gmail.com.
authors.forename: Tarsila Ferraz
authors.initials: TF
authors.lastname: Frezza
authors.affiliation: Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
authors.forename: Ana Luiza Ribeiro
authors.initials: AL
authors.lastname: de Souza
authors.affiliation: Departamento de Biologia Animal, Laboratório de Helmintologia, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: César Corat Ribeiro
authors.initials: CC
authors.lastname: Prado
authors.affiliation: Departamento de Biologia Animal, Laboratório de Helmintologia, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: Claudineide Nascimento Fernandes
authors.initials: CN
authors.lastname: de Oliveira
authors.affiliation: Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
authors.forename: Maria Palmira Daflon
authors.initials: MP
authors.lastname: Gremião
authors.affiliation: Departamento de Biologia Animal, Laboratório de Helmintologia, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: Selma
authors.initials: S
authors.lastname: Giorgio
authors.affiliation: Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: Mary Anne Heidi
authors.initials: MA
authors.lastname: Dolder
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: Paulo Pinto
authors.initials: PP
authors.lastname: Joazeiro
authors.affiliation: Departamento de Biologia Animal, Laboratório de Helmintologia, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: Silmara Marques
authors.initials: SM
authors.lastname: Allegretti
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D003106:Colon / Q000469:parasitology; D004334:Drug Administration Schedule; D004337:Drug Carriers / Q000008:administration & dosage; D004353:Drug Evaluation, Preclinical; D005260:Female; D006931:Hyperbaric Oxygenation; D008081:Liposomes / Q000008:administration & dosage; D008297:Male; D051379:Mice; D010058:Oviposition / Q000187:drug effects / Q000502:physiology; D010270:Parasite Egg Count; D011223:Praziquantel / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000502:physiology; D012555:Schistosomiasis mansoni / Q000188:drug therapy*
chemical_list: D000871:Anthelmintics; D004337:Drug Carriers; D008081:Liposomes; D011223:Praziquantel
keywords: Hiperbaric-oxigen; In vivo assay; Liposomal-praziquantel; Praziquantel; Schistosoma mansoni