title: [Determination of scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in plants of Erycibe].
abstract: OBJECTIVE
  An accurate and reliable analytical method for-simultaneous determination of six active components (scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) in plants of Erycibe was developed.
  
  
  METHODS
  Scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C in the samples were well separated in analytical HPLC by gradual elution with methanol-0.1% formic acid solution. The chromatographic condictions: Agilent Poroshell 120 EC-C18 column, flowing rate being 1 mL x min(-1), detecting wavelength at 345 nm.
  
  
  RESULTS
  Good linearities of scopolin, chlorogenic acid, scopoletin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C were in the range of 0.026 8-2.68, 0.027 0-2.70, 0.008 1-0.81, 0.018 8-1.88, 0.017 6-1.76, 0.019 6-1.96 μg, respectively (r > 0.999 6). The average recoveries of the six components were 98.1%, 98.7%, 100.8%, 100.4%, 99.7%, 101.1%; the relative standard deviations were 2.67%, 2.86%, 2.62%, 1.98%, 2.76%, 2.19%.
  
  
  CONCLUSIONS
  The method is simple, feasible and reproducible and can be used for the quality control of plants of Erycibe.
authors.forename: Xiao-kun
authors.initials: XK
authors.lastname: Xu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Chen
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Liao
authors.forename: Zi-jia
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
mesh_terms: D002681:China; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027722:Convolvulaceae / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D012603:Scopoletin / Q000032:analysis*
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C469310:isochlorogenic acid; C417572:scopolin; D002726:Chlorogenic Acid; D012603:Scopoletin