title: Cell spreading area regulates clathrin-coated pit dynamics on micropatterned substrate.
abstract: Clathrin-mediated endocytosis (CME) is the most characterized pathway for the endocytic entry of proteins and lipids at the plasma membrane of eukaryotic cells. Numerous studies have probed the roles of different endocytic accessory proteins in regulating the dynamics of clathrin-coated pit (CCP) assembly. However, it is not completely clear how physical cues regulate CCP dynamics. Here we employ microcontact printing to control cell shape and examine CCP dynamics as a function of cell spreading area for three differently sized cells. Cells with a large spreading area had more short-lived CCPs but a higher CCP initiation rate. Interestingly, we found that fluorescence intensity of CCPs decreased with increasing cell spreading area in a manner that was dependent on the cortical actin network. Our results point to another facet of the regulation of CCP dynamics, suggesting that CME may be modulated while cells change their mechanical state and remodel their actin cytoskeleton during various processes.
authors.affiliation: Department of Mechanical Engineering, University of Michigan, 2350 Hayward Street, Ann Arbor, Michigan MI 48105, USA. allenliu@umich.edu.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Tan
authors.forename: Johanna
authors.initials: J
authors.lastname: Heureaux
authors.forename: Allen P
authors.initials: AP
authors.lastname: Liu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D002966:Clathrin / Q000378:metabolism*; D022163:Clathrin-Coated Vesicles / Q000502:physiology*; D003034:Coated Pits, Cell-Membrane / Q000502:physiology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D002966:Clathrin; D004129:Dimethylpolysiloxanes; C013830:baysilon