title: Effects of lipid composition on the properties of doxorubicin-loaded liposomes.
abstract: BACKGROUND
  The liposomal lipid composition of doxorubicin-loaded liposome likely will influence its pharmacological activity. Results & methodology: We prepared 18 formulations of doxorubicin-loaded liposomes in which the lipid composition was varied. It was indicated that the intracellular uptake of doxorubicin is the primary property of doxorubicin-loaded liposome that affects its cytotoxicity in vitro. Furthermore, the release rate of doxorubicin from liposome and the biological activity of the lipid itself also affected the cytotoxicity.
  
  
  CONCLUSIONS
  These findings provide an insight into how lipid composition influences the cytotoxicity of the doxorubicin-loaded liposomes. Our results provide valuable information that should help to enhance the therapeutic efficacy of liposomal anticancer drug products by optimizing their formulations.
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakai-Kato
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Kunie
authors.initials: K
authors.lastname: Nanjo
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kawanishi
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Okuda
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004317:Doxorubicin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000903:Antibiotics, Antineoplastic; D008055:Lipids; D008081:Liposomes; C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D004317:Doxorubicin; D000255:Adenosine Triphosphate