title: Antimicrobial Peptide, Lumbricusin, Ameliorates Motor Dysfunction and Dopaminergic Neurodegeneration in a Mouse Model of Parkinson's Disease.
abstract: We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of Parkinson's disease (PD). In MNSCs isolated from mouse brains, Lumbricusin treatment significantly increased cell proliferation (up to 12%) and reduced the protein expression of p27(Kip1) through proteasomal protein degradation but not transcriptional regulation. Lumbricusin inhibited the 6-OHDA-induced apoptosis of MNSCs, and also showed neuroprotective effects in a mouse PD model, ameliorating the motor impairments seen in the pole, elevated body swing, and rotation tests. These results suggest that the Lumbricusin-induced promotion of neural cell proliferation via p27(Kip1) degradation has a protective effect in an experimental PD model. Thus, the antimicrobial peptide Lumbricusin could possibly be developed as a potential therapeutic agent for the treatment of PD.
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Ik Hwan
authors.initials: IH
authors.lastname: Lee
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Seung Taek
authors.initials: ST
authors.lastname: Nam
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Ji
authors.initials: J
authors.lastname: Hong
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Li Fang
authors.initials: LF
authors.lastname: Lu
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Clinical Research Institute, Daejeon St. Mary's Hospital, The Catholic University of Korea, Daejeon 301-723, Republic of Korea.
authors.forename: Ki Cheol
authors.initials: KC
authors.lastname: Park
authors.affiliation: Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon 487-711, Republic of Korea.
authors.forename: Ho
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects*; D015801:Helminth Proteins / Q000008:administration & dosage / Q000378:metabolism*; D051379:Mice; D000068079:Motor Disorders / Q000188:drug therapy*; D058953:Neural Stem Cells / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000378:metabolism*; D010300:Parkinson Disease / Q000188:drug therapy* / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D023181:Antimicrobial Cationic Peptides; D015801:Helminth Proteins; D018696:Neuroprotective Agents; C000590837:lumbricusin peptide, Lumbricus terrestris
keywords: Antimicrobial peptide; Parkinson's disease,; cell cycle arrest; cell proliferation (neurotropine); neuroprotective activity; proteasomal protein degradation