title: A fluorescent G-quadruplex probe for the assay of base excision repair enzyme activity.
abstract: A sensitive and selective assay of uracil DNA glycosylase (UDG) activity is developed by utilizing a G-quadruplex probe incorporating 2-aminopurine (2-AP), based on a novel design that excision reaction promoted by UDG triggers the formation of G-quadruplex structure with significant fluorescence enhancement of 2-AP within the probe.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21 + Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. hgpark@kaist.ac.kr.
authors.forename: Chang Yeol
authors.initials: CY
authors.lastname: Lee
authors.forename: Ki Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
mesh_terms: D015075:2-Aminopurine / Q000737:chemistry; D004260:DNA Repair; D057075:Enzyme Assays / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D051981:Uracil-DNA Glycosidase / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D015075:2-Aminopurine; D051981:Uracil-DNA Glycosidase