title: Iron binding to caseins in the presence of orthophosphate.
abstract: As adding >5mM ferric chloride to sodium caseinate solutions results in protein precipitation, the effects of orthophosphate (0-64 mM) addition to sodium caseinate solution (2% w/v protein) on iron-induced aggregation of the caseins were studied at pH 6.8. Up to 20mM ferric chloride could be added to sodium caseinate solution containing 32 mM orthophosphate without any protein precipitation. The addition of iron to sodium caseinate solution containing orthophosphate reduced the diffusible phosphorus content in a concentration-dependent manner. Added iron appeared to interact simultaneously with phosphoserine on the caseins and inorganic phosphorus. The relative sizes of the casein aggregates were governed by the concentration of orthophosphate and the aggregates consisted of all casein fractions, even at the lowest level of ferric chloride addition (5mM). It is hypothesised that the addition of iron to caseins in the presence of orthophosphate results in the formation of colloidal structures involving casein-iron-orthophosphate interactions.
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4474, New Zealand.
authors.forename: V A
authors.initials: VA
authors.lastname: Mittal
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4474, New Zealand.
authors.forename: A
authors.initials: A
authors.lastname: Ellis
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4474, New Zealand.
authors.forename: A
authors.initials: A
authors.lastname: Ye
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11 222, Palmerston North 4474, New Zealand.
authors.forename: P J B
authors.initials: PJB
authors.lastname: Edwards
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4474, New Zealand.
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4474, New Zealand. Electronic address: h.singh@massey.ac.nz.
authors.forename: H
authors.initials: H
authors.lastname: Singh
mesh_terms: D002364:Caseins / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D010768:Phosphoserine / Q000737:chemistry*
chemical_list: D002364:Caseins; D010710:Phosphates; D010768:Phosphoserine; D007501:Iron
keywords: Acetone (PubChem CID:180); Beta-mercaptoethanol (PubChem CID:1567); Brilliant blue (PubChem CID:19700); Casein; Deuterium oxide (PubChem CID:24602); Di-potassium hydrogen orthophosphate (PubChem CID:16217523); Ferric chloride hexahydrate (PubChem CID:24810); Glacial acetic acid (PubChem CID:176); Immidazole (PubChem CID:795); Iron; Orthophosphate; Phosphoserine; Propanol (PubChem CID:1031); Sodium hydroxide (PubChem CID:14798); Solubility; Triphenulphosphine (PubChem CID:11776)