title: [Study on anti-inflammation and immunoloregulation effect of Guizhi Fuling capsule ingredients using high content screening].
abstract: The present study sought to investigate the anti-inflammation and immunoloregulation effect of 17 Guizhi Fuling capsule ingredients. The anti-inflammatory ingredients on LPS-induced RAW264. 7 cell injury were assessed with ELISA and immunofluorescence. The release of IL-1β, TNF-α, PGE2 were detected with ELISA and the expression of COX-2 was detected with immunofluorescence. The effects of them on promoting splenic lymphocyte proliferation were assessed with MTT and Hoechst 33342 staining method. The results showed that 15 ingredients had obviously anti-inflammatory activity on LPS- induced injury and play the immunoloregulation roles. This study suggested that the 15 ingredients may be the active ingredients on pelvic infection.
authors.forename: Yan-ru
authors.initials: YR
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.forename: Chen-feng
authors.initials: CF
authors.lastname: Zhang
authors.forename: Tuan-jie
authors.initials: TJ
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.forename: Zhen-zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002214:Capsules / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D007155:Immunologic Factors / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D002214:Capsules; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; C525079:guizhi-fuling; D051546:Cyclooxygenase 2