title: LACK OF ASSOCIATION BETWEEN HERPESVIRUS DETECTION IN SALIVA AND GINGIVITIS IN HIV‑INFECTED CHILDREN.
abstract: The aims of this study were to compare the detection of human herpesviruses (HHVs) in the saliva of HIV-infected and healthy control children, and to evaluate associations between viral infection and gingivitis and immunodeficiency. Saliva samples were collected from 48 HIV-infected and 48 healthy control children. Clinical and laboratory data were collected during dental visits and from medical records. A trained dentist determined gingival indices and extension of gingivitis. Saliva samples were tested for herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), and cytomegalovirus (CMV) by nested polymerase chain reaction assays. Thirty-five HIV-infected and 16 control children had gingivitis. Seventeen (35.4%) HIV-infected children and 13 (27%) control children were positive for HHVs. CMV was the most commonly detected HHV in both groups (HIV-infected, 25%; control, 12.5%), followed by HSV-1 (6.2% in both groups) and HSV-2 (HIV-infected, 4.2%; control, 8.3%). The presence of HHVs in saliva was not associated with the presence of gingivitis in HIV-1-infected children (p = 0.104) or healthy control children (p = 0.251), or with immunosuppression in HIV-infected individuals (p = 0.447). Gingivitis was correlated with HIV infection (p = 0.0001). These results suggest that asymptomatic salivary detection of HHVs is common in HIV-infected and healthy children, and that it is not associated with gingivitis.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Renata A
authors.initials: RA
authors.lastname: Otero
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Flávia N N
authors.initials: FN
authors.lastname: Nascimento
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Ivete P R
authors.initials: IP
authors.lastname: Souza
authors.affiliation: Department of Virology, Microbiology Institute, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Raquel C
authors.initials: RC
authors.lastname: Silva
authors.affiliation: Department of Virology, Microbiology Institute, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Lima
authors.affiliation: Department of Virology, Microbiology Institute, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Tatiana F
authors.initials: TF
authors.lastname: Robaina
authors.affiliation: Department of Virology, Microbiology Institute, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Fernando P
authors.initials: FP
authors.lastname: Câmara
authors.affiliation: Department of Virology, Microbiology Institute, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Norma
authors.initials: N
authors.lastname: Santos
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Gloria F
authors.initials: GF
authors.lastname: Castro
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000821:virology*; D058345:Asymptomatic Infections; D016022:Case-Control Studies; D002648:Child; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005891:Gingivitis / Q000175:diagnosis / Q000821:virology*; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000821:virology*
chemical_list: D004279:DNA, Viral