title: P2 of Rice grassy stunt virus (RGSV) and p6 and p9 of Rice ragged stunt virus (RRSV) isolates from Vietnam exert suppressor activity on the RNA silencing pathway.
abstract: In Vietnam, the two main viruses that cause disease in rice are the Rice grassy stunt virus (RGSV) and the Rice ragged stunt virus (RRSV). Outbreaks of these two viruses have dramatically decreased rice production in Vietnam. Because natural resistance genes are unknown, an RNAi strategy may be an alternative method to develop resistance to RGSV and RRSV. However, this strategy will be efficient only if putative silencing suppressors encoded by the two viruses are neutralized. To identify these suppressors, we used the classical green fluorescent protein (GFP) agroinfiltration method in Nicotiana benthamiana. Then, we investigated the effects of viral candidate proteins on GFP expression and GFP siRNA accumulation and their interference with the short- or long-range signal of silencing. RGSV genes s2gp1, s5gp2, and s6gp1 and RRSV genes s5gp1, s6gp1, s9gp1, and s10gp1 were selected for viral silencing suppressor investigation according to their small molecular weight, the presence of cysteines, or the presence of a GW motif in related protein products. We confirmed that protein p6 of RRSV displays mild silencing suppressor activity and affects long-range silencing by delaying the systemic silencing signal. In addition, we identified two new silencing suppressors that displayed mild activity: p2 of RGSV and p9 of RRSV.
authors.affiliation: IRD, UMR IPME, Avenue Agropolis, 34398, Montpellier Cedex, France.|IRD, LMI RICE, Agricultural Genetics Institute, Université des Sciences et Techniques d'Hanoi, Hanoi, Vietnam.
authors.forename: Thanh Duc
authors.initials: TD
authors.lastname: Nguyen
authors.affiliation: IRD, UMR IPME, Avenue Agropolis, 34398, Montpellier Cedex, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Lacombe
authors.affiliation: IRD, UMR IPME, Avenue Agropolis, 34398, Montpellier Cedex, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Bangratz
authors.affiliation: Plant Protection Research Institute (PPRI), Hanoi, Vietnam.
authors.forename: Hoang Anh
authors.initials: HA
authors.lastname: Ta
authors.affiliation: IRD, LMI RICE, Agricultural Genetics Institute, Université des Sciences et Techniques d'Hanoi, Hanoi, Vietnam.
authors.forename: Do Nang
authors.initials: do N
authors.lastname: Vinh
authors.affiliation: IRD, LMI RICE, Agricultural Genetics Institute, Université des Sciences et Techniques d'Hanoi, Hanoi, Vietnam.|Université de Montpellier, UMR DIADE, Bat 15, CC 002, Place Eugène Bataillon, 34095, Montpellier Cedex 5, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Gantet
authors.affiliation: IRD, UMR IPME, Avenue Agropolis, 34398, Montpellier Cedex, France. christophe.brugidou@ird.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Brugidou
mesh_terms: D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D054884:Host-Pathogen Interactions; D012275:Oryza / Q000821:virology; D034622:RNA Interference; D012087:Reoviridae / Q000276:immunology* / Q000502:physiology*; D029364:Tenuivirus / Q000276:immunology* / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D014744:Vietnam; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D049452:Green Fluorescent Proteins
keywords: Rice grassy stunt virus; Rice production in Vietnam; Rice ragged stunt virus; Rice viruses; Silencing suppressors