title: Bacillus radicibacter sp. nov., a new bacterium isolated from root nodule of Oxytropis ochrocephala Bunge.
abstract: A Gram-positive, facultative anaerobic, rod-shaped, and endospore-forming strain, designated 53-2(T) was isolated from the root nodule of Oxytropis ochrocephala Bunge growing on Qilian mountain, China. The strain can grow at pH 7.0-8.0, 10-50 °C and tolerate up to 11% NaCl. Optimal growth occurred at pH 7.2 and 37 °C. The result of BLASTn search based on 16S rRNA gene sequence revealed that strain 53-2(T) , being closest related to Bacillus acidicola 105-2(T) , possessed remote similarity (less than 95.64%) to the species within genus Bacillus. The DNA G + C content was 37.8%. Chemotaxonomic data (major quinone is MK-7; major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipid, and aminoglycophospholipid; fatty acids are anteiso-C15: 0 , iso-C15:0 and anteiso-C17: 0 ) supported the affiliation of the isolate to the genus Bacillus. On the basis of physiological, phylogenetic, and biochemical properties, strain 53-2(T) represents a novel species within genus Bacillus, for which the name Bacillus radicibacter is proposed. The type strain is 53-2(T) (=DSM27302(T) =ACCC06115(T) =CCNWQLS5(T) ).
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Xiu Li
authors.initials: XL
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Yan Bing
authors.initials: YB
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Meng Sha
authors.initials: MS
authors.lastname: Han
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Dan Hong
authors.initials: DH
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Wei Min
authors.initials: WM
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Ge Hong
authors.initials: GH
authors.lastname: Wei
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D033021:Oxytropis / Q000382:microbiology*; D010715:Phosphatidylglycerols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: Bacillus radicibacter sp. nov; Root nodule