title: Evaluation of Sealing Ability of Biodentine as Retrograde Filling Material by Using two Different Manipulation Methods: An In Vitro Study.
abstract: BACKGROUND
  The study was aimed to evaluate the microleakage of Biodentine using two different manipulation methods by dye penetration.
  
  
  METHODS
  A total of 60 single-rooted human maxillary permanent teeth were cleaned and obturated with gutta-percha using lateral condensation method. Standardized root-end cavities were prepared after apical resection. All teeth were divided randomly into two groups of 30 specimens and were filled with Biodentine by trituration and hand manipulation methods. The samples were coated with varnish and immersed in 1% methylene blue dye for 72 h. Then the teeth were sectioned longitudinally and observed under a stereomicroscope. The depth of dye penetration was measured in millimeters.
  
  
  RESULTS
  There was highly statistical significant difference observed between Group I and Group II (P < 0.001) when dye penetration scores were compared.
  
  
  CONCLUSIONS
  More microleakage was seen when Biodentine was manually manipulated as compared to machine trituration.
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry & Endodontics, Rungta College of Dental Sciences & Research, Bhilai, Chhattisgarh, India.
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Gupta
authors.affiliation: Post-graduate Student, Department of Conservative Dentistry & Endodontics, Rungta College of Dental Sciences & Research, Bhilai, Chhattisgarh, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Garg
authors.affiliation: Reader, Department of Conservative Dentistry & Endodontics, Regional Dental College, Guwahati, Assam, India.
authors.forename: Chandana
authors.initials: C
authors.lastname: Kalita
authors.affiliation: Professor and Head, Department of Oral Pathology & Microbiology, Regional Dental College, Guwahati, Assam, India.
authors.forename: Anjan
authors.initials: A
authors.lastname: Saikia
authors.affiliation: Associate Professor, Department of Periodontology, Rungta College of Dental Sciences & Research, Bhilai, Chhattisgarh, India.
authors.forename: T S
authors.initials: TS
authors.lastname: Srinivasa
authors.affiliation: Associate Professor, Department of Conservative Dentistry and Endodontics, Darshan Dental College & Hospital, Loyara, Udaipur, Rajasthan, India.
authors.forename: G
authors.initials: G
authors.lastname: Satish
keywords: Anterior teeth; biodentine; microleakage; root-end filling; trituration