title: Chemical Fluorescent Probe for Detection of Aβ Oligomers.
abstract: Aggregation of amyloid β-peptide (Aβ) is implicated in the pathology of Alzheimer's disease (AD), with the soluble, Aβ oligomeric species thought to be the critical pathological species. Identification and characterization of intermediate species formed during the aggregation process is crucial to the understanding of the mechanisms by which oligomeric species mediate neuronal toxicity and following disease progression. Probing these species proved to be extremely challenging, as evident by the lack of reliable sensors, due to their heterogeneous and transient nature. We describe here an oligomer-specific fluorescent chemical probe, BoDipy-Oligomer (BD-Oligo), developed through the use of the diversity-oriented fluorescent library approach (DOFLA) and high-content, imaging-based screening. This probe enables dynamic oligomer monitoring during fibrillogenesis in vitro and shows in vivo Aβ oligomers staining possibility in the AD mice model.
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology and Research (A*STAR) , 11 Biopolis Way, 02-02 Helios, Biopolis, Singapore 138667, Singapore.
authors.forename: Chai Lean
authors.initials: CL
authors.lastname: Teoh
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology and Research (A*STAR) , 11 Biopolis Way, 02-02 Helios, Biopolis, Singapore 138667, Singapore.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Su
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology and Research (A*STAR) , 11 Biopolis Way, 02-02 Helios, Biopolis, Singapore 138667, Singapore.
authors.forename: Srikanta
authors.initials: S
authors.lastname: Sahu
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology and Research (A*STAR) , 11 Biopolis Way, 02-02 Helios, Biopolis, Singapore 138667, Singapore.
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Yun
authors.forename: Eleanor
authors.initials: E
authors.lastname: Drummond
authors.forename: Frances
authors.initials: F
authors.lastname: Prelli
authors.affiliation: Department of Chemistry and the Center for Fluctuating Thermodynamics, Sookmyung Women's University , Cheongpa-ro 47-gil 100, Yongsan-Ku, Seoul 140-742, Korea.
authors.forename: Sulgi
authors.initials: S
authors.lastname: Lim
authors.affiliation: Department of Chemistry and the Center for Fluctuating Thermodynamics, Sookmyung Women's University , Cheongpa-ro 47-gil 100, Yongsan-Ku, Seoul 140-742, Korea.
authors.forename: Sunhee
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Chemistry and the Center for Fluctuating Thermodynamics, Sookmyung Women's University , Cheongpa-ro 47-gil 100, Yongsan-Ku, Seoul 140-742, Korea.
authors.forename: Sihyun
authors.initials: S
authors.lastname: Ham
authors.forename: Thomas
authors.initials: T
authors.lastname: Wisniewski
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology and Research (A*STAR) , 11 Biopolis Way, 02-02 Helios, Biopolis, Singapore 138667, Singapore.|Department of Chemistry and MedChem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D000818:Animals; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D013816:Thermodynamics
chemical_list: D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes