title: Taxonomic description and genome sequence of Rheinheimera mesophila sp. nov., isolated from an industrial waste site.
abstract: A novel Gram-staining-negative gammaproteobacterium, designated IITR-13T, was isolated from a pesticide-contaminated soil and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, the strain showed the closest similarity (98.7 %) to Rheinheimera tangshanensis JA3-B52T followed by Rheinheimera texasensis A62-14BT (97.7 %) and Rheinheimera soli BD-d46T (97.3 %). The 16S rRNA gene sequence similarity of the novel strain to other members of the genus Rheinheimera was < 97.3 %. However, DNA-DNA hybridization between strain IITR-13T and the type strains of R. tangshanensis, R. texasensis and R. soli was 47.5 ± 0.6, 42.4 ± 0.4 and 39.8 ± 0.3 %, respectively; these values are less than 70 %, a threshold value for delineation of a novel species. The strain had C12 : 0 3-OH, C16 : 0, C17 : 1ω8c, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c) and C18 : 1ω6c as the major fatty acids. The major isoprenoid quinones detected for strain IITR-13T were ubiquinone Q-8 and menaquinone MK-7.The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and seven unknown phospholipids. Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the novel strain should be assigned to a novel species, for which the name Rheinheimera mesophila sp. nov. is proposed, with the type strain IITR-13T ( = MTCC 12064T = DSM 29723T). Also, we report the draft genome sequence of Rheinheimera mesophila IITR-13T; the draft genome sequence includes 3 749 903 bases and comprises 3449 predicted coding sequences, with a G+C content of 47.8 %. It consists of 102 contigs (>1000 bp).
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160 036, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Environmental Biotechnology Division, CSIR-Indian Institute of Toxicological Research (IITR), Lucknow 226 001, India.
authors.forename: Abhay
authors.initials: A
authors.lastname: Bajaj
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160 036, India.
authors.forename: Rajendran
authors.initials: R
authors.lastname: Mathan Kumar
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160 036, India.
authors.forename: Gurwinder
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160 036, India.
authors.forename: Navjot
authors.initials: N
authors.lastname: Kaur
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160 036, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Kumar Singh
authors.affiliation: Environmental Biotechnology Division, CSIR-Indian Institute of Toxicological Research (IITR), Lucknow 226 001, India.
authors.forename: Natesan
authors.initials: N
authors.lastname: Manickam
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160 036, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002841:Chromatiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010575:Pesticides; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D007220:Industrial Waste; D010575:Pesticides; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants