title: Origin of the Outbreak in France of Pseudomonas syringae pv. actinidiae Biovar 3, the Causal Agent of Bacterial Canker of Kiwifruit, Revealed by a Multilocus Variable-Number Tandem-Repeat Analysis.
abstract: The first outbreaks of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae biovar 3 were detected in France in 2010. P. syringae pv. actinidiae causes leaf spots, dieback, and canker that sometimes lead to the death of the vine. P. syringae pv. actinidifoliorum, which is pathogenic on kiwi as well, causes only leaf spots. In order to conduct an epidemiological study to track the spread of the epidemics of these two pathogens in France, we developed a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA). MLVA was conducted on 340 strains of P. syringae pv. actinidiae biovar 3 isolated in Chile, China, France, Italy, and New Zealand and on 39 strains of P. syringae pv. actinidifoliorum isolated in Australia, France, and New Zealand. Eleven polymorphic VNTR loci were identified in the genomes of P. syringae pv. actinidiae biovar 3 ICMP 18744 and of P. syringae pv. actinidifoliorum ICMP 18807. MLVA enabled the structuring of P. syringae pv. actinidiae biovar 3 and P. syringae pv. actinidifoliorum strains in 55 and 16 haplotypes, respectively. MLVA and discriminant analysis of principal components revealed that strains isolated in Chile, China, and New Zealand are genetically distinct from P. syringae pv. actinidiae strains isolated in France and in Italy, which appear to be closely related at the genetic level. In contrast, no structuring was observed for P. syringae pv. actinidifoliorum. We developed an MLVA scheme to explore the diversity within P. syringae pv. actinidiae biovar 3 and to trace the dispersal routes of epidemic P. syringae pv. actinidiae biovar 3 in Europe. We suggest using this MLVA scheme to trace the dispersal routes of P. syringae pv. actinidiae at a global level.
authors.affiliation: UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 Quasav, Institut National de la Recherche Agronomique, Beaucouzé, France Laboratoire de la Santé des Végétaux, Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Angers, France.
authors.forename: A
authors.initials: A
authors.lastname: Cunty
authors.affiliation: UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 Quasav, Institut National de la Recherche Agronomique, Beaucouzé, France.
authors.forename: S
authors.initials: S
authors.lastname: Cesbron
authors.affiliation: Laboratoire de la Santé des Végétaux, Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Angers, France.
authors.forename: F
authors.initials: F
authors.lastname: Poliakoff
authors.affiliation: UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 Quasav, Institut National de la Recherche Agronomique, Beaucouzé, France.
authors.forename: M-A
authors.initials: MA
authors.lastname: Jacques
authors.affiliation: Laboratoire de la Santé des Végétaux, Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Angers, France charles.manceau@anses.fr.
authors.forename: C
authors.identifier: http://orcid.org/0000-0003-3590-6454
authors.initials: C
authors.lastname: Manceau
mesh_terms: D029042:Actinidia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D004196:Disease Outbreaks; D005602:France / Q000453:epidemiology; D005638:Fruit / Q000382:microbiology; D014644:Genetic Variation; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology* / Q000706:statistics & numerical data; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*