title: ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds.
abstract: We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality ≥10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real-time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas.
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil. ; Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, São Paulo, SP, Brazil.
authors.forename: Kleber A
authors.initials: KA
authors.lastname: Gomes
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil.
authors.forename: Tiago C
authors.initials: TC
authors.lastname: Almeida
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil. ; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Mandioca e Fruticultura Tropical, Cruz das Almas, Bahia, Brazil.
authors.forename: Abelmon S
authors.initials: AS
authors.lastname: Gesteira
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Grupo Bioenergia e Meio Ambiente, Ilhéus, Bahia, Brazil.
authors.forename: Ivon P
authors.initials: IP
authors.lastname: Lôbo
authors.affiliation: Fundação Oswaldo Cruz (FIOCRUZ), Instituto Oswaldo Cruz (IOC), Laboratório de Genômica Funcional e Bioinformática, Rio de Janeiro, RJ, Brazil.
authors.forename: Ana Carolina R
authors.initials: AC
authors.lastname: Guimarães
authors.affiliation: Fundação Oswaldo Cruz (FIOCRUZ), Instituto Oswaldo Cruz (IOC), Laboratório de Genômica Funcional e Bioinformática, Rio de Janeiro, RJ, Brazil.
authors.forename: Antonio B
authors.initials: AB
authors.lastname: de Miranda
authors.affiliation: Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, São Paulo, SP, Brazil.
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Van Sluys
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Grupo Bioenergia e Meio Ambiente, Ilhéus, Bahia, Brazil.
authors.forename: Rosenira S
authors.initials: RS
authors.lastname: da Cruz
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil.
authors.forename: Júlio Cm
authors.initials: JC
authors.lastname: Cascardo
authors.affiliation: Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil. ; Fundação Oswaldo Cruz (FIOCRUZ), Instituto Oswaldo Cruz (IOC), Laboratório de Genômica Funcional e Bioinformática, Rio de Janeiro, RJ, Brazil.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Carels
keywords: Jatropha curcas; alkaloids; biofuel; fatty acids; genomics; terpenes