title: Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin.
abstract: While neurogenic stem cells have been identified in rodent and human skin, their manipulation and further characterization are hampered by a lack of specific markers. Here, we perform genetic tracing of the progeny of boundary cap (BC) cells, a neural-crest-derived cell population localized at peripheral nerve entry/exit points. We show that BC derivatives migrate along peripheral nerves to reach the skin, where they give rise to terminal glia associated with dermal nerve endings. Dermal BC derivatives also include cells that self-renew in sphere culture and have broad in vitro differentiation potential. Upon transplantation into adult mouse dorsal root ganglia, skin BC derivatives efficiently differentiate into various types of mature sensory neurons. Together, this work establishes the embryonic origin, pathway of migration, and in vivo neurogenic potential of a major component of skin stem-like cells. It provides genetic tools to study and manipulate this population of high interest for medical applications.
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Gresset
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Coulpier
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France; Sorbonne Universités, UPMC Université Paris 06, IFD, 4 Place Jussieu, 75252 Paris Cedex 05, France.
authors.forename: Gaspard
authors.initials: G
authors.lastname: Gerschenfeld
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France; Sorbonne Universités, UPMC Université Paris 06, IFD, 4 Place Jussieu, 75252 Paris Cedex 05, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Jourdon
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France.
authors.forename: Graziella
authors.initials: G
authors.lastname: Matesic
authors.affiliation: National Reference Centre "Rare Peripheral Neuropathies" Department of Neurology, Centre Hospitalier Universitaire de Limoges, 87042 Limoges, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Richard
authors.affiliation: National Reference Centre "Rare Peripheral Neuropathies" Department of Neurology, Centre Hospitalier Universitaire de Limoges, 87042 Limoges, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Vallat
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France. Electronic address: patrick.charnay@ens.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Charnay
authors.affiliation: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France.
authors.forename: Piotr
authors.initials: P
authors.lastname: Topilko
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002465:Cell Movement; D002478:Cells, Cultured; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology; D055495:Neurogenesis; D009457:Neuroglia / Q000166:cytology*; D011984:Sensory Receptor Cells / Q000166:cytology; D012867:Skin / Q000166:cytology*