title: In Vitro/In Vivo Evaluation of Dexamethasone--PAMAM Dendrimer Complexes for Retinal Drug Delivery.
abstract: Current treatment options for diabetic retinopathy (DR) have side effects because of invasive application and topical application does not generally result in therapeutic levels in the target tissue. Therefore, improving the drug delivery to retina, following topical administration, might be a solution to DR treatment problems. The purpose of this study was to investigate the complexation effects of poly(amidoamine) (PAMAM) dendrimers on ocular absorption of dexamethasone (DEX). Using different PAMAM generations, complex formulations were prepared and characterized. Formulations were evaluated in terms of cytotoxicity and cell permeability, as well as ex vivo transport across ocular tissues. The ocular pharmacokinetic properties of DEX formulations were studied in Sprague-Dawley rats following topical and subconjunctival applications, to evaluate the effect of PAMAM on retinal delivery of DEX. Methyl-thiazol-tetrazolium (MTT) assay indicated that all groups resulted in cell viability comparable to DEX solution (87.5%), with the cell viability being the lowest for G3 complex at 73.5%. Transport study results showed that dendrimer complexation increases DEX transport across both cornea and sclera tissues. The results of in vivo studies were also indicated that especially anionic DEX-PAMAM complex formulations have reached higher DEX concentrations in ocular tissues compared with plain DEX suspension.
authors.affiliation: Hacettepe University Faculty of Pharmacy, Pharmaceutical Technology Department, Ankara, Turkey.
authors.forename: Burçin
authors.initials: B
authors.lastname: Yavuz
authors.affiliation: Hacettepe University Faculty of Pharmacy, Pharmaceutical Technology Department, Ankara, Turkey. Electronic address: sbozdag@hacettepe.edu.tr.
authors.forename: Sibel Bozdagğ
authors.initials: SB
authors.lastname: Pehlivan
authors.affiliation: Hacettepe University Faculty of Pharmacy, Pharmaceutical Technology Department, Ankara, Turkey.
authors.forename: İmran
authors.initials: İ
authors.lastname: Vural
authors.affiliation: Hacettepe University Faculty of Pharmacy, Pharmaceutical Technology Department, Ankara, Turkey.
authors.forename: Nurşen
authors.initials: N
authors.lastname: Ünlü
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D050091:Dendrimers / Q000737:chemistry*; D003907:Dexamethasone / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D010539:Permeability; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D050091:Dendrimers; C104700:PAMAM Starburst; D003907:Dexamethasone
keywords: cell culture; dendrimers; drug delivery systems; drug transport; nanotechnology; ophthalmic drug delivery; permeability