title: AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues.
abstract: Plants synthesize secondary metabolites, including flavonoids, which play important role during various stresses for their survival. These metabolites are also considered as health-protective components in functional foods. Flavonols, one of the important groups of flavonoids, apart from performing several roles in plants have been recognized as potent phytoceuticals for human health. Tomato fruits are deficient in this group of flavonoids and have been an important target for enhancing the accumulation of flavonols through genetic manipulations. In the present study, AtMYB12 transcription factor of the Arabidopsis has been expressed under constitutive promoter in tomato. Transgenic tomato lines exhibited enhanced accumulation of flavonols and chlorogenic acid (CGA) in leaf and fruit accompanied with elevated expression of phenylpropanoid pathway genes involved in flavonol biosynthesis. In addition, global gene expression analysis in leaf and fruit suggested that AtMYB12 modulates number of molecular processes including aromatic amino acid biosynthesis, phytohormone signaling and stress responses. Besides this, a differential modulation of the genes in fruits and leaves is reported in this study. Taken together, results demonstrate that modulation of primary carbon metabolism and other pathways by AtMYB12 in tomato may lead to sufficient substrate supply for enhanced content of phenolics in general and flavonols in particular.
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Prashant
authors.initials: P
authors.lastname: Misra
authors.affiliation: CSIR-Central Drug Research Institute (CSIR-CDRI), Endocrinology Division, Jankipuram Extension, Sitapur Road, Lucknow; 226021, INDIA.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Choudhary
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Reena
authors.initials: R
authors.lastname: Yadav
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Ridhi
authors.initials: R
authors.lastname: Goel
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Sweta
authors.initials: S
authors.lastname: Bhambhani
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Indraneel
authors.initials: I
authors.lastname: Sanyal
authors.affiliation: CSIR-Central Drug Research Institute (CSIR-CDRI), Endocrinology Division, Jankipuram Extension, Sitapur Road, Lucknow; 226021, INDIA.
authors.forename: Ritu
authors.initials: R
authors.lastname: Trivedi
authors.affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow; 226 001, INDIA.
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D005419:Flavonoids / Q000096:biosynthesis* / Q000235:genetics; D005638:Fruit / Q000235:genetics / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D005419:Flavonoids; C504539:MYB12 protein, Arabidopsis; D014157:Transcription Factors