title: Modification in the properties of paper by using cellulase-free xylanase produced from alkalophilic Cellulosimicrobium cellulans CKMX1 in biobleaching of wheat straw pulp.
abstract: Alkalophilic Cellulosimicrobium cellulans CKMX1 isolated from mushroom compost is an actinomycete that produces industrially important and environmentally safer thermostable cellulase-free xylanase, which is used in the pulp and paper industry as an alternative to the use of toxic chlorinated compounds. Strain CKMX1 was previously characterized by metabolic fingerprinting, whole-cell fatty acids methyl ester analysis, and 16S rDNA and was found to be C. cellulans CKMX1. Crude enzyme (1027.65 U/g DBP) produced by C. cellulans CKMX1, having pH and temperature optima of 8.0 and 60 °C, respectively, in solid state fermentation of apple pomace, was used in the production of bleached wheat straw pulp. Pretreatment with xylanase at a dose of 5 U/g after pulping decreased pulp kappa points by 1.4 as compared with the control. Prebleaching with a xylanase dose of 5 U/g pulp reduced the chlorine charge by 12.5%, increased the final brightness points by approximately 1.42% ISO, and improved the pulp strength properties. Xylanase could be substituted for alkali extraction in C-Ep-D sequence and used for treating chemically bleached pulp, resulting in bleached pulp with higher strength properties. Modification of bleached pulp with 5 U of enzyme/g increased pulp whiteness and breaking length by 1.03% and 60 m, respectively; decreased tear factor of pulp by 7.29%; increased bulk weight by 3.99%, as compared with the original pulp. Reducing sugars and UV-absorbing lignin-derived compound values were considerably higher in xylanase-treated samples. Cellulosimicrobium cellulans CKMX1 has a potential application in the pulp and paper industries.
authors.affiliation: a Department of Microbiology, DAV University, Jalandhar 144001, Punjab, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Walia
authors.affiliation: b Centre for Advance Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Mehta
authors.affiliation: c Department of Basic Sciences, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173230, Himachal Pradesh, India.
authors.forename: Shiwani
authors.initials: S
authors.lastname: Guleria
authors.affiliation: c Department of Basic Sciences, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173230, Himachal Pradesh, India.
authors.forename: Chand Karan
authors.initials: CK
authors.lastname: Shirkot
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D002480:Cellulase / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D008031:Lignin / Q000737:chemistry; D010209:Paper; D018547:Plant Stems / Q000737:chemistry; D013696:Temperature; D014908:Triticum / Q000737:chemistry*
chemical_list: D008031:Lignin; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: HPLC; brightness; degré de blancheur; indice kappa; kappa number; paper; papier; pulping; xylose