title: Establishment of a new animal model of azithromycin-induced liver injury and study the molecular pathological change during the process.
abstract: The purpose of the present study is to establish a new animal model of azithromycin (AZ)-induced liver injury and study the molecular pathological change during the process. First, mice were respectively injected intraperitoneally with AZ of different high doses. Our results showed that 800 mg/kg AZ injection significantly induced liver injury in the mice, which reflected an ideal process of liver injury and repair. In this study, we analyzed the molecular pathological changes during the process by hematoxylin and eosin staining, immunohistochemistry, Western blot, and quantitative real-time reverse transcription polymerase chain reaction in the liver of mice at 0, 12, 24, 48, and 72 h after 800 mg/kg injection. Our results showed that the expression of heat shock protein 70, proliferating cell nuclear antigen, vascular endothelial growth factor, caspase 3, and cytochrome P450 2E1 were significantly differently expressed during liver injury induced by 800 mg/kg AZ in mice. Our results will be conducive for further study of the pathogenesis and prevention of drug-induced liver injury.
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, People's Republic of China sanqiangli2001@163.com.
authors.forename: S-Q
authors.initials: SQ
authors.lastname: Li
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, People's Republic of China.
authors.forename: X-D
authors.initials: XD
authors.lastname: Wan
authors.affiliation: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhu
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, People's Republic of China.
authors.forename: H-M
authors.initials: HM
authors.lastname: Han
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, People's Republic of China.
authors.forename: Z-S
authors.initials: ZS
authors.lastname: Xu
authors.affiliation: The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang, People's Republic of China.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Lu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D017963:Azithromycin / Q000633:toxicity*; D015153:Blotting, Western; D053148:Caspase 3 / Q000235:genetics; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D018840:HSP70 Heat-Shock Proteins; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D017963:Azithromycin; D019392:Cytochrome P-450 CYP2E1; D053148:Caspase 3
keywords: Azithromycin; HSP70; PCNA; VEGF; caspase 3; drug-induced liver injury