title: Isolation of Methoxyfuranocoumarins From Ammi majus by Centrifugal Partition Chromatography.
abstract: Pure methoxyfuranocoumarins were isolated from Ammi majus L. by use of low-pressure column chromatography (LPCC) followed by centrifugal partition chromatography (CPC). The concentrated petroleum ether extract from fruits of A. majus was fractionated on a silica gel column using a gradient of ethyl acetate in dichloromethane (0-80%, v/v). Coumarin-rich fractions were analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography with diode array detection (HPLC/DAD). Xanthotoxin (8-MOP) and isopimpinellin (isoP), structurally similar compounds, were isolated in one fraction (FR6). To avoid multistep and long-lasting TLC preparation, optimization of CPC conditions has been performed. In one run, an effective separation of 8-MOP and isoP was achieved. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10 : 8 : 10 : 9; v/v) in an ascending mode (the aqueous phase was a stationary phase, and the organic phase was a mobile phase), with flow rate 3 mL/min and rotation speed 1,600 r.p.m., was used. The identification and high purities of isolated 8-MOP (98.7%) and isoP (100%) were confirmed by HPLC/DAD assay, when compared with standards. The developed CPC method could be applied to the effective isolation of 8-MOP and isoP from plant extracts. The high purity of obtained compounds makes possible further exploitation of these components in biological studies.
authors.affiliation: Chair and Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodźki 1 Str., Lublin 20-093, Poland mbartnik@pharmacognosy.org.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bartnik
authors.affiliation: Chair and Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodźki 1 Str., Lublin 20-093, Poland.
authors.forename: Anna Katarzyna
authors.initials: AK
authors.lastname: Mazurek
mesh_terms: D031165:Ammi / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification*
chemical_list: D003374:Coumarins