title: Identification and expression analysis of the LRR-RLK gene family in tomato (Solanum lycopersicum) Heinz 1706.
abstract: As the largest subfamily of receptor-like kinases (RLKs), leucine-rich repeat receptor-like kinases (LRR-RLKs) regulate the growth, development, and stress responses of plants. Through a reiterative process of sequence analysis and re-annotation, 234 LRR-RLK genes were identified in the genome of tomato (Solanum lycopersicum) 'Heinz 1706', which were further grouped into 10 major groups based on their sequence similarity. In comparison to the significant role of tandem duplication in the expansion process of this gene family in other species, only approximately 12% (29 out of 234) of SlLRR-RLK genes arose from tandem duplication. Using the multiple expectation maximization for motif elicitation (MEME) method, the motif composition and arrangement were found to be variably conserved within each SlLRR-RLK group, indicating their different extent of functional divergence. Expression profiling analyses by qRT-PCR data revealed that SlLRR-RLK genes were differentially expressed in various tomato organs and tissues, and some SlLRR-RLK genes exhibited preferential expression in fruits at distinct developmental stages, suggesting that SlLRR-RLK may take important roles in fruit development and ripening process. The results of this study provide an overview of the LRR-RLK gene family in tomato Heinz 1706, one important species of Solanaceae, and will be helpful for future functional analysis of this important protein family in fleshy fruit-bearing species.
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.|School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Wei
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.|School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Jiehua
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.|School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Yang
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.|School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Yingjin
authors.initials: Y
authors.lastname: Song
mesh_terms: D020816:Amino Acid Motifs; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D000090263:Leucine-Rich Repeat Proteins; D018551:Solanum lycopersicum / Q000235:genetics*; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010770:Phosphotransferases / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics*
chemical_list: D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins; D010770:Phosphotransferases
keywords: Solanum lycopersicum; analyse d’expression; analyse phylogénétique; expression profiling; identification de motifs; leucine-rich repeat receptor-like kinase; motif elicitation; phylogenetic analysis; récepteur kinase riche en leucines