title: Nanoparticle corona for proteins: mechanisms of interaction between dendrimers and proteins.
abstract: Protein absorption at the surface of big nanoparticles and formation of 'protein corona' can completely change their biological properties. In contrast, we have studied the binding of small nanoparticles - dendrimers - to proteins and the formation of their 'nanoparticle corona'. Three different types of interactions were observed. (1) If proteins have rigid structure and active site buried deeply inside, the 'nanoparticle corona' is unaffected. (2) If proteins have a flexible structure and their active site is also buried deeply inside, the 'nanoparticle corona' affects protein structure, but not enzymatic activity. (3) The 'nanoparticle corona' changes both the structure and enzymatic activity of flexible proteins that have surface-based active centers. These differences are important in understanding interactions taking place at a bio-nanointerface.
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja st. 27, 220072 Minsk, Belarus. Electronic address: shcharbin@gmail.com.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska st. 141/133, 90-236 Lodz, Poland.
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja st. 27, 220072 Minsk, Belarus.
authors.forename: Viktar
authors.initials: V
authors.lastname: Abashkin
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja st. 27, 220072 Minsk, Belarus.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Loznikova
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja st. 27, 220072 Minsk, Belarus.
authors.forename: Volha
authors.initials: V
authors.lastname: Dzmitruk
authors.affiliation: Republican Research and Practical Center of Neurology and Neurosurgery, Skoriny st. 24, 220114 Minsk, Belarus.
authors.forename: Natallia
authors.initials: N
authors.lastname: Shcharbina
authors.affiliation: Republican Research and Practical Center of Neurology and Neurosurgery, Skoriny st. 24, 220114 Minsk, Belarus.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Matusevich
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska st. 141/133, 90-236 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Milowska
authors.affiliation: Institute of General Food Chemistry, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, ul. Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Krystian
authors.initials: K
authors.lastname: Gałęcki
authors.affiliation: Institute of General Food Chemistry, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, ul. Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Wysocki
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska st. 141/133, 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D002942:Circular Dichroism; D050091:Dendrimers / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D011506:Proteins
keywords: Corona; Dendrimers; Interactions; Mechanisms; Nanoparticles; Proteins