title: Protocols to test the activity of antimicrobial peptides against the honey bee pathogen Paenibacillus larvae.
abstract: Paenibacillus larvae is the causal agent of the honey bee disease American Foulbrood. Two enhanced protocols that allow the activity of antimicrobial peptides to be tested against P. larvae are presented. Proof of principle experiments demonstrate that the honey bee antimicrobial peptide defensin 1 is active in both assays.
authors.affiliation: School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: smithj23@unlv.nevada.edu.
authors.forename: Jasmin C
authors.initials: JC
authors.lastname: Khilnani
authors.affiliation: School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: helen.wing@unlv.edu.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Wing
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001516:Bees / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods*; D056507:Paenibacillus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides
keywords: American Foulbrood disease; Antimicrobial compounds; Honey bee; Paenibacillus larvae