title: A calibration curve for immobilized dihydrofolate reductase activity assay.
abstract: An assay was developed for measuring the active-site concentration, activity, and thereby the catalytic turnover rate (k cat) of an immobilized dihydrofolate reductase model system (Singh et al., (2015), Anal. Biochem). This data article contains a calibration plot for the developed assay. In the calibration plot rate is plotted as a function of DHFR concentration and shows linear relationship. The concentration of immobilized enzyme was varied by using 5 different size mica chips. The dsDNA concentration was the same for all chips, assuming that the surface area of the mica chip dictates the resulting amount of bound enzyme (i.e. larger sized chip would have more bound DHFR). The activity and concentration of each chip was measured.
authors.affiliation: The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Holly
authors.initials: H
authors.lastname: Morris
authors.affiliation: The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Tivanski
authors.affiliation: The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Amnon
authors.initials: A
authors.lastname: Kohen
keywords: Activity assay; Dihydrofolate reductase