title: Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots.
abstract: Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. h.j.cooper@bham.ac.uk.
authors.forename: Rian L
authors.initials: RL
authors.lastname: Griffiths
authors.forename: Alex
authors.initials: A
authors.lastname: Dexter
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Creese
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000328:Adult; D059788:Dried Blood Spot Testing / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D008055:Lipids / Q000097:blood / Q000302:isolation & purification; D059625:Liquid-Liquid Extraction / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D013499:Surface Properties
chemical_list: D006454:Hemoglobins; D008055:Lipids