title: Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity.
abstract: Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatography, SDS-PAGE and mass-spectrometry, 128 proteins with potentially different abundances in patient groups were identified, 62 of the 128 proteins are mainly localized in the mitochondria. Further quantification of 12 of these 62 proteins by immune dot blot analysis revealed four proteins citrate synthase, HADHA, LETM1 and mitofilin being inversely associated with BMI, and mitofilin being inversely correlated with gender.
authors.affiliation: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital, University of Basel, Basel CH-4031, Switzerland.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Lindinger
authors.affiliation: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital, University of Basel, Basel CH-4031, Switzerland.
authors.forename: Martine
authors.initials: M
authors.lastname: Christe
authors.affiliation: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital, University of Basel, Basel CH-4031, Switzerland ; Collegium Helveticum, ETH Zurich, Zurich CH-8092, Switzerland.
authors.forename: Alex N
authors.initials: AN
authors.lastname: Eberle
authors.affiliation: Department of Surgery, St. Claraspital, Basel CH-4059, Switzerland.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Kern
authors.affiliation: Department of Surgery, St. Claraspital, Basel CH-4059, Switzerland.
authors.forename: Ralph
authors.initials: R
authors.lastname: Peterli
authors.affiliation: Interdisciplinary Center of Nutritional and Metabolic Diseases, St. Claraspital, Kleinriehenstrasse 30, Basel CH-4058, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Peters
authors.affiliation: MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors.forename: Kamburapola J I
authors.initials: KJ
authors.lastname: Jayawardene
authors.affiliation: MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Fearnley
authors.affiliation: MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors.forename: John E
authors.initials: JE
authors.lastname: Walker