title: Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 & EMBS082 by 16S rRNA Gene Sequencing.
abstract: 16S rDNA sequencing which has gained wide popularity amongst microbiologists for the molecular characterization and identification of newly discovered isolates provides accurate identification of isolates down to the level of sub-species (strain). Its most important advantage over the traditional biochemical characterization methods is that it can provide an accurate identification of strains with atypical phenotypic characters as well. The following work is an application of 16S rRNA gene sequencing approach to identify a novel species of Probiotic Lactobacillus acidophilus. The sample was collected from pond water samples of rural and urban areas of Krishna district, Vijayawada, Andhra Pradesh, India. Subsequently, the sample was serially diluted and the aliquots were incubated for a suitable time period following which the suspected colony was subjected to 16S rDNA sequencing. The sequence aligned against other species was concluded to be a novel, Probiotic L. acidophilus bacteria, further which were named L. acidophilus strain EMBS081 & EMBS082. After the sequence characterization, the isolate was deposited in GenBank Database, maintained by the National Centre for Biotechnology Information NCBI. The sequence can also be retrieve from EMBL and DDBJ repositories with accession numbers JX255677 and KC150145.
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: Harshpreet
authors.initials: H
authors.lastname: Chandok
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: Pratik
authors.initials: P
authors.lastname: Shah
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: Uday Raj
authors.initials: UR
authors.lastname: Akare
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: Maliram
authors.initials: M
authors.lastname: Hindala
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: Sneha Singh
authors.initials: SS
authors.lastname: Bhadoriya
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: G V
authors.initials: GV
authors.lastname: Ravi
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India.
authors.forename: Varsha
authors.initials: V
authors.lastname: Sharma
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Bandaru
authors.affiliation: Department of Biotechnology, Sanghvi Institute of Management & Science, Indore, 453 331, India.
authors.forename: Pragya
authors.initials: P
authors.lastname: Rathore
authors.affiliation: Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India. anuraj@eminentbio.com.
authors.forename: Anuraj
authors.initials: A
authors.lastname: Nayarisseri
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D007779:Lactobacillus acidophilus / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16srRNA sequencing; Lactobacillus acidophilus; Lactobacillus acidophilus strain EMBS081; Lactobacillus acidophilus strain EMBS082; Probiotic Lactobacillus