title: Effects of triploidy incidence on clinical outcomes for IVF-ET cycles in different ovarian stimulation protocols.
abstract: OBJECTIVE
  To discuss the relationship between triploidy incidence and clinical outcomes of embryos derived from normally fertilized oocytes from the same cohort for in vitro fertilization-embryo transfer (IVF-ET) cycles in different ovarian stimulation protocol.
  
  
  METHODS
  This study included 2070 in vitro fertilization (IVF) cycles with long-term protocol, 802 IVF cycles with ultra short-term protocol and 508 IVF-D (in vitro fertilization by donor semen) cycles with long-term protocol from January 2013 to September 2014. According to the different 3PN rate, patients were divided into three groups as follows: Group 1 included patients with 0% 3PN zygotes, Group 2 included patients with 1-25% 3PN zygotes and Group 3 included patients with >25% 3PN zygotes.
  
  
  METHODS
  female age, no. of retrieved oocytes, normal fertilization rate, day-3 grade I + II embryos rate, day-3 grade I + II + III embryos rate, implantation rate, pregnancy rate and early abortion rate.
  
  
  RESULTS
  Triploidy cycle incidence rate in IVF and IVF-D cycles with long-term protocol were significantly higher than in IVF cycles with ultra short-term protocol (p < 0.001). Triploidy fertilization rate found no significant difference between the three groups (p > 0.05). In three protocols, normal fertilization rate in 3PN = 0% and 3PN = 1-25% groups were significantly higher compared to 3PN > 25% group (p < 0.001). In IVF cycles with long-term protocol, the day-3 grade I + II embryos, implantation and pregnancy rate in 3PN > 25% group were significantly lower than other two groups (p < 0.05). The day-3 grade I + II + III embryos and early abortion rate found no significant difference between the three groups (p > 0.05). In IVF cycles with ultra short-term protocol, there were no significant differences found in day-3 grade I + II embryos, day-3 grade I + II + III embryos, implantation, pregnancy and early abortion rate (p > 0.05). In IVF-D cycles with long-term protocol, the day-3 grade I + II embryos, day-3 grade I + II + III embryos and implantation rate in 3PN > 25% group were significantly lower than other two groups (p < 0.05). The pregnancy and early abortion rates found no significant difference in the three groups (p > 0.05).
  
  
  CONCLUSIONS
  We observed that high proportion of triploid zygotes made a negative effect on clinical outcomes for IVF-ET cycles with long-term protocol.
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Mingzhao
authors.initials: M
authors.lastname: Li
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xue
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Silin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Ren
authors.affiliation: a The ART Center, Maternal and Child Health Care Hospital of Shaanxi Province , Xi'an , China.
authors.forename: Juanzi
authors.initials: J
authors.lastname: Shi
mesh_terms: D000328:Adult; D010064:Embryo Implantation / Q000502:physiology*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D015994:Incidence; D008297:Male; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D018873:Pregnancy Rate; D016896:Treatment Outcome; D057885:Triploidy
keywords: Clinical outcomes; IVF; triploidy