title: The Oncogenic Response to MiR-335 Is Associated with Cell Surface Expression of Membrane-Type 1 Matrix Metalloproteinase (MT1-MMP) Activity.
abstract: MicroRNA miR-335 has been reported to have both tumor suppressor and oncogenic activities. In order to determine possible tissue and cell type differences in response to miR-335, we examined the effect of miR-335 on cell expression of MT1-MMP, a proteinase commonly expressed in tumors and associated with cell proliferation and migration. miR-335 increased cell surface expression of MT1-MMP in fibrosarcoma HT-1080 and benign prostate BPH-1 cells, but not in prostate LNCaP or breast MCF-7 tumor cells. miR-335 stimulated proliferation and cell migration in a wound healing in vitro assay in HT-1080, BPH-1, and U87 glioblastoma cells, cells which demonstrated significant cell surface expression of MT1-MMP. In contrast, miR-335 did not affect proliferation or migration in cells without a prominent plasma membrane associated MT1-MMP activity. Our data suggest that differences in response to miR-335 by tumor cells may lie in part in the mechanism of regulation of MT1-MMP production.
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Fausto
authors.initials: F
authors.lastname: Rojas
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Hernandez
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Milagros
authors.initials: M
authors.lastname: Silva
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Lihua
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis, Minnesota, United States of America; Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Subbaya
authors.initials: S
authors.lastname: Subramanian
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America; Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America; Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, United States of America; Minneapolis VA Medical Center, Minneapolis, Minnesota, United States of America.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Wilson
authors.affiliation: Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America; Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D061986:MCF-7 Cells; D053511:Matrix Metalloproteinase 14 / Q000235:genetics* / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D018613:Microscopy, Confocal; D009857:Oncogenes / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004792:Enzyme Precursors; C556347:MIRN335 microRNA, human; D035683:MicroRNAs; D020778:Matrix Metalloproteinase 2; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14