title: pKa tuning in quadrupolar-type two-photon ratiometric fluorescent membrane probes.
abstract: Two bolaamphiphilic quadrupoles built from a fluorene core conjugated with azine endgroups were designed and successfully used to stain GUV membranes. Their quadrupolar character induces both a shift of the pKa values close to the physiological pH and large two-photon absorption responses (i.e. over 1000 GM for acidic forms). As such they hold promise as ratiometric two-photon pH probes for monitoring slight variations of pH near cell membranes using two-photon excitation in the NIR region.
authors.affiliation: Université de Bordeaux, ISM, UMR 5255 CNRS, F-33400 Talence, France. mireille.blanchard-desce@u-bordeaux.fr.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Daniel
authors.forename: Cristiano
authors.initials: C
authors.lastname: Mastrodonato
authors.forename: Aude
authors.initials: A
authors.lastname: Sourdon
authors.forename: Guillaume
authors.initials: G
authors.lastname: Clermont
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Vabre
authors.forename: Bertrand
authors.initials: B
authors.lastname: Goudeau
authors.forename: Hannah
authors.initials: H
authors.lastname: Voldoire
authors.forename: Stéphane
authors.initials: S
authors.lastname: Arbault
authors.forename: Olivier
authors.initials: O
authors.lastname: Mongin
authors.forename: Mireille
authors.initials: M
authors.lastname: Blanchard-Desce
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry*; D011522:Protons
chemical_list: D005456:Fluorescent Dyes; D010743:Phospholipids; D011522:Protons