title: Accumulation of transcription factors and cell signaling-related proteins in the nucleus during citrus-Xanthomonas interaction.
abstract: The nucleus is the maestro of the cell and is involved in the modulation of cell signaling during stress. We performed a comprehensive nuclear proteome analysis of Citrus sinensis during interaction with host (Xanthomonas citri pv. citri-Xcc) and non-host (Xanthomonas oryzae pv. oryzae-Xoo) pathogens. The nuclear proteome was obtained using a sequential method of organelle enrichment and determined by nano-LC-MS/MS analysis. A total of 243 proteins accumulated differentially during citrus-Xanthomonas interaction, belonging to 11 functional groups, with signaling and transcription-related proteins dominating. MADS-box transcription factors, DEAD-box RNA helicase and leucine aminopeptidase, mainly involved in jasmonic acid (JA) responses, were in high abundance during non-host interaction (Xoo). Signaling-related proteins like serine/threonine kinase, histones (H3.2, H2A), phosphoglycerate kinase, dynamin, actin and aldolase showed increased accumulation early during Xoo interaction. Our results suggest that there is a possible involvement of JA-triggered defense responses during non-host resistance, with early recognition of the non-host pathogen.
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, Telangana, India.
authors.forename: T Swaroopa
authors.initials: TS
authors.lastname: Rani
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, Telangana, India.
authors.forename: P
authors.initials: P
authors.lastname: Durgeshwar
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, Telangana, India. Electronic address: arpsl@uohyd.ernet.in.
authors.forename: Appa Rao
authors.initials: AR
authors.lastname: Podile
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D032084:Citrus sinensis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D003517:Cyclopentanes / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D057865:Plant Immunity; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014974:Xanthomonas / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D020543:Proteome; D014157:Transcription Factors; C011006:jasmonic acid
keywords: 2DE; Citrus; Nano-LC MS/MS; Pathogens; Proteome analysis; Xanthomonas