title: Renoprotective mechanisms of morin in cisplatin-induced kidney injury.
abstract: In this study, we investigated the renoprotective effects of morin on cisplatin-induced kidney injury in mice. Serum creatinine and blood urea nitrogen (BUN) levels, glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities were determined according to the corresponding kits. The mRNA levels of TNF-α and IL-1β in kidney tissues were measured by quantitative real-time PCR (qRT-PCR). The activities of cytochrome P450 2E1 (CYP2E1), nuclear factor kappa B (NF-κB) p65, P38 mitogen-activated protein kinase (MAPK), Bax, p53 and cleaved caspase 3 were evaluated by western blotting. The results showed that the model of cisplatin-induced kidney injury was successfully replicated, and morin significantly attenuated histopathological changes and decreased the levels of TNF-α and IL-1β in the kidneys. In addition, morin attenuated the activation of CYP2E1, phospho-NF-κB p65, phospho-P38 MAPK, Bax, phospho-p53 and cleaved caspase 3 in CP-induced kidney injury. In conclusion, these results indicated that the renoprotective mechanisms of morin may be attributed to the suppression of oxidative stress, inflammation and apoptosis in CP-induced kidney injury.
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China. Electronic address: wei_zhengkai@126.com.
authors.forename: Zhengkai
authors.initials: Z
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Xuexiu
authors.initials: X
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Kou
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Libin
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Minjun
authors.initials: M
authors.lastname: Yao
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Ershun
authors.initials: E
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Changming
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China. Electronic address: yangzhengtao01@sina.com.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000009:adverse effects*; D003404:Creatinine / Q000097:blood; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism; D047309:Flavones
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin; D003404:Creatinine; D005419:Flavonoids; D005979:Glutathione Peroxidase; D020011:Protective Agents; D013482:Superoxide Dismutase; C008548:morin; D047309:Flavones
keywords: Apoptosis; Cisplatin; Inflammation; Kidney injury; Morin; Oxidative stress