title: Detection of rotavirus species A, B and C in domestic mammalian animals with diarrhoea and genotyping of bovine species A rotavirus strains.
abstract: Rotaviruses (RVs) are a major cause of neonatal diarrhoea in humans and animals worldwide. In this study, 425 faecal samples were collected between 1999 and 2013 from diarrhoeic livestock and companion animals at different locations in Germany and tested for RVs. A previously published real-time RT-PCR assay was optimized for detection of a larger variety of RV species A (RVA) strains, and real-time RT-PCR assays for detection of RV species B (RVB) and C (RVC) were newly developed. The detection limits of the assays were 1.54×10(2), 3.95×10(2) and 3.60×10(3) genome copies for RVA, RVB and RVC, respectively. RVA was identified in 85.2% of bovine samples, 51.2% of porcine samples, 50.0% of feline samples, 43.2% of equine samples and 39.7% of canine samples. RVB was found in 3.0% of bovine samples, 2.7% of equine samples and 1.6% of porcine samples. RVC was detected in 31.0% of porcine samples, 21.7% of feline samples, 9.0% of canine samples and 6.0% of bovine samples. For genotyping, 101 RVA-positive bovine samples were further analysed by semi-nested RT-PCR. Genotype combination G6P[5] was most frequently detected (67.3% of samples), followed by G6P[11] (13.9%), G10P[5] (4.0%), G8P[11] (3.0%), G6P[1] (1.0%), and G10P[11] (1.0%). Mixed RVA infections were detected in 5.9% of samples; no or incomplete typing was possible in 4.0% of the samples. This first overview on RV species and RVA genotypes in diarrhoeic livestock and companion animals from Germany indicates a broad circulation of a large variety of RVs.
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Straße 96a, 07743 Jena, Germany.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Otto
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Straße 96a, 07743 Jena, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Rosenhain
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Straße 96a, 07743 Jena, Germany.
authors.forename: Mandy C
authors.initials: MC
authors.lastname: Elschner
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Straße 96a, 07743 Jena, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Hotzel
authors.affiliation: Federal Institute for Risk Assessment, Max-Dohrn-Strasse 8-10, 10609 Berlin, Germany.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Machnowska
authors.affiliation: Federal Institute for Risk Assessment, Max-Dohrn-Strasse 8-10, 10609 Berlin, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Trojnar
authors.affiliation: Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen Sachsen, Jägerstraße 10, 01099 Dresden, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Hoffmann
authors.affiliation: Federal Institute for Risk Assessment, Max-Dohrn-Strasse 8-10, 10609 Berlin, Germany. Electronic address: Reimar.Johne@bfr.bund.de.
authors.forename: Reimar
authors.initials: R
authors.lastname: Johne
mesh_terms: D000820:Animal Diseases / Q000453:epidemiology / Q000821:virology*; D000818:Animals; D000829:Animals, Domestic; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D060085:Coinfection / Q000662:veterinary; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D006736:Horses; D008322:Mammals; D008969:Molecular Sequence Data; D057805:Pets; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine
keywords: Animal rotavirus; Genotyping; RVA; RVB; RVC; Real-time RT-PCR; Rotavirus