title: An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering.
abstract: Current nerve tissue engineering applications are adopting xenogeneic nerve tissue as potential nerve grafts to help aid nerve regeneration. However, there is little literature that describes the exact location, anatomy and physiology of these nerves to highlight their potential as a donor graft. The aim of this study was to identify and characterise the structural and extracellular matrix (ECM) components of porcine peripheral nerves in the hind leg. Methods included the dissection of porcine nerves, localisation, characterisation and quantification of the ECM components and identification of nerve cells. Results showed a noticeable variance between porcine and rat nerve (a commonly studied species) in terms of fascicle number. The study also revealed that when porcine peripheral nerves branch, a decrease in fascicle number and size was evident. Porcine ECM and nerve fascicles were found to be predominately comprised of collagen together with glycosaminoglycans, laminin and fibronectin. Immunolabelling for nerve growth factor receptor p75 also revealed the localisation of Schwann cells around and inside the fascicles. In conclusion, it is shown that porcine peripheral nerves possess a microstructure similar to that found in rat, and is not dissimilar to human. This finding could extend to the suggestion that due to the similarities in anatomy to human nerve, porcine nerves may have utility as a nerve graft providing guidance and support to regenerating axons.
authors.affiliation: Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK.
authors.forename: Leyla
authors.initials: L
authors.lastname: Zilic
authors.affiliation: School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
authors.forename: Philippa E
authors.initials: PE
authors.lastname: Garner
authors.affiliation: Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK.
authors.forename: Tong
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK.
authors.forename: Sabiniano
authors.initials: S
authors.lastname: Roman
authors.affiliation: Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK.
authors.forename: John W
authors.initials: JW
authors.lastname: Haycock
authors.affiliation: School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
authors.forename: Stacy-Paul
authors.initials: SP
authors.lastname: Wilshaw
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D003094:Collagen / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis; D007797:Laminin / Q000032:analysis; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D010525:Peripheral Nerves / Q000033:anatomy & histology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000502:physiology; D013552:Swine; D013718:Tensile Strength / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D005353:Fibronectins; D006025:Glycosaminoglycans; D007797:Laminin; D003094:Collagen
keywords: extracellular matrix; peripheral nerve; porcine; ultrastructure