title: The poly(A)-binding protein Nab2 functions in RNA polymerase III transcription.
abstract: RNA polymerase III (RNAPIII) synthesizes most small RNAs, the most prominent being tRNAs. Although the basic mechanism of RNAPIII transcription is well understood, recent evidence suggests that additional proteins play a role in RNAPIII transcription. Here, we discovered by a genome-wide approach that Nab2, a poly(A)-binding protein important for correct poly(A) tail length and nuclear mRNA export, is present at all RNAPIII transcribed genes. The occupancy of Nab2 at RNAPIII transcribed genes is dependent on transcription. Using a novel temperature-sensitive allele of NAB2, nab2-34, we show that Nab2 is required for the occupancy of RNAPIII and TFIIIB at target genes. Furthermore, Nab2 interacts with RNAPIII, TFIIIB, and RNAPIII transcripts. Importantly, impairment of Nab2 function causes an RNAPIII transcription defect in vivo and in vitro. Taken together, we establish Nab2, an important mRNA biogenesis factor, as a novel player required for RNAPIII transcription by stabilizing TFIIIB and RNAPIII at promoters.
authors.affiliation: Institute of Biochemistry, Justus Liebig University Giessen, 35392 Giessen, Germany; Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany;
authors.forename: L Maximilian
authors.initials: LM
authors.lastname: Reuter
authors.affiliation: Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany;
authors.forename: Dominik M
authors.initials: DM
authors.lastname: Meinel
authors.affiliation: Institute of Biochemistry, Justus Liebig University Giessen, 35392 Giessen, Germany; Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany; Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-University Munich, 81377 Munich, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Sträßer
mesh_terms: D015966:Gene Expression Regulation, Fungal; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C080773:NAB2 protein, S cerevisiae; D029543:Nucleocytoplasmic Transport Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D012320:RNA Polymerase III
keywords: Nab2; RNA polymerase III; TFIIIB; gene expression; ncRNA; tRNA