title: A laser-based technology for fabricating a soda-lime glass based microfluidic device for circulating tumour cell capture.
abstract: We developed a laser-based technique for fabricating microfluidic microchips on soda-lime glass substrates. The proposed methodology combines a laser direct writing, as a manufacturing tool for the fabrication of the microfluidics structures, followed by a post-thermal treatment with a CO2 laser. This treatment will allow reshaping and improving the morphological (roughness) and optical qualities (transparency) of the generated microfluidics structures. The use of lasers commonly implemented for material processing makes this technique highly competitive when compared with other glass microstructuring approaches. The manufactured chips were tested with tumour cells (Hec 1A) after being functionalized with an epithelial cell adhesion molecule (EpCAM) antibody coating. Cells were successfully arrested on the pillars after being flown through the device giving our technology a translational application in the field of cancer research.
authors.affiliation: Microoptics and GRIN Optics Group, Applied Physics Department, Faculty of Physics, University of Santiago de Compostela, Santiago de Compostela E15782, Spain. Electronic address: daniel.nieto@usc.es.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nieto
authors.affiliation: Translational Medical Oncology, Health Research Institute of Santiago (IDIS), Fundacion Ramon Dominguez, SERGAS, 15706 Santiago de Compostela, Spain. Electronic address: Ramiro.Couceiro.Otero@sergas.es.
authors.forename: Ramiro
authors.initials: R
authors.lastname: Couceiro
authors.affiliation: Microoptics and GRIN Optics Group, Applied Physics Department, Faculty of Physics, University of Santiago de Compostela, Santiago de Compostela E15782, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Aymerich
authors.affiliation: Translational Medical Oncology, Health Research Institute of Santiago (IDIS), Fundacion Ramon Dominguez, SERGAS, 15706 Santiago de Compostela, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Lopez-Lopez
authors.affiliation: Translational Medical Oncology, Health Research Institute of Santiago (IDIS), Fundacion Ramon Dominguez, SERGAS, 15706 Santiago de Compostela, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Abal
authors.affiliation: Microoptics and GRIN Optics Group, Applied Physics Department, Faculty of Physics, University of Santiago de Compostela, Santiago de Compostela E15782, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Flores-Arias
mesh_terms: D017610:Calcium Compounds; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000097:blood / Q000473:pathology; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices; D007834:Lasers; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010087:Oxides; D012972:Sodium Hydroxide
chemical_list: D017610:Calcium Compounds; D010087:Oxides; C004569:soda lime; D012972:Sodium Hydroxide
keywords: Circulating tumour cells; Glass materials; Laser ablation; Microfluidics devices