title: Degradation of corn stover by fungal cellulase cocktail for production of polyhydroxyalkanoates by moderate halophile Paracoccus sp. LL1.
abstract: Bioprocessing of lignocellulose as a renewable resource for fuels, chemicals or value added products is a necessity to fulfil demands of petroleum products. This study aims to convert corn stover to polyhydroxyalkanoates (PHA). Corn stover was hydrolyzed to crude sugars by an on-site prepared cellulase cocktail from co-culture of Trichoderma reesei and Aspergillus niger. The potent PHA producer, Paracoccus sp. LL1, was isolated from Lonar Lake, India and could accumulate PHA up to 72.4% of its dry cell weight. PHA production reached 9.71 g/L from corn stover hydrolysate containing 40 g/L sugar mixture. The PHA synthase gene (phaC) sequence of the isolate showed 79% identity with the phaC gene of Paracoccus seriniphilus (E71) strain from the NCBI database. The nature/type of PHA was found to be poly(3-hydroxybutyrate) by Fourier transform infrared spectroscopy.
authors.affiliation: Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 362-763, Republic of Korea.
authors.forename: Shailesh S
authors.initials: SS
authors.lastname: Sawant
authors.affiliation: Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 362-763, Republic of Korea; School of Life Sciences, North Maharashtra University, Post Box 80, Jalgaon 425001, Maharashtra, India.
authors.forename: Bipinchandra K
authors.initials: BK
authors.lastname: Salunke
authors.affiliation: Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 362-763, Republic of Korea. Electronic address: bskim@chungbuk.ac.kr.
authors.forename: Beom Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002244:Carbon / Q000032:analysis; D002480:Cellulase / Q000378:metabolism*; D005798:Genes, Bacterial; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis; D010230:Paracoccus / Q000378:metabolism*; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D002480:Cellulase; D009584:Nitrogen
keywords: Corn stover; Fungal cellulase cocktail; PHA synthase gene (phaC); Paracoccus sp. LL1; Polyhydroxyalkanoates