title: Distinct 18S rRNA precursors are targets of the exosome complex, the exoribonuclease RRP6L2 and the terminal nucleotidyltransferase TRL in Arabidopsis thaliana.
abstract: The biosynthesis of ribosomal RNA and its incorporation into functional ribosomes is an essential and intricate process that includes production of mature ribosomal RNA from large precursors. Here, we analyse the contribution of the plant exosome and its co-factors to processing and degradation of 18S pre-RNAs in Arabidopsis thaliana. Our data show that, unlike in yeast and humans, an RRP6 homologue, the nucleolar exoribonuclease RRP6L2, and the exosome complex, together with RRP44, function in two distinct steps of pre-18S rRNA processing or degradation in Arabidopsis. In addition, we identify TRL (TRF4/5-like) as the terminal nucleotidyltransferase that is mainly responsible for oligoadenylation of rRNA precursors in Arabidopsis. We show that TRL is required for efficient elimination of the excised 5' external transcribed spacer and of 18S maturation intermediates that escaped 5' processing. Our data also suggest involvement of additional nucleotidyltransferases, including terminal uridylyltransferase(s), in modifying rRNA processing intermediates in plants.
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.|Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5a, 02-106, Warsaw, Poland.
authors.forename: Pawel J
authors.initials: PJ
authors.lastname: Sikorski
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Zuber
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.
authors.forename: Lucas
authors.initials: L
authors.lastname: Philippe
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.
authors.forename: François M
authors.initials: FM
authors.lastname: Sement
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Canaday
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5a, 02-106, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kufel
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Gagliardi
authors.affiliation: Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, University of Strasbourg, 67000, Strasbourg, France.
authors.forename: Heike
authors.initials: H
authors.lastname: Lange
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D063326:Exosome Multienzyme Ribonuclease Complex / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; D012322:RNA Precursors; D012337:RNA, Ribosomal, 18S; D009713:Nucleotidyltransferases; C000608382:TRL protein, Arabidopsis; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C000608381:RRP6L2 protein, Arabidopsis
keywords: 18S rRNA processing; Arabidopsis thaliana; RNA exosome; RRP6L2; polyadenylation; terminal nucleotidyltransferase; uridylation