title: Acute and sub-chronic toxicity of Tri-sa-maw recipe in rats.
abstract: BACKGROUND
  Tri-sa-maw recipe is a botanical preparation comprised of equal proportions ofthe three herbalfruits, namely Terminalia chebula Retz., Tenninalia sp. and Terminalia bellirica Roxb. This recipe is used for antipyretic, expectorant, periodic maintenance, and relieving stomach tight.
  
  
  OBJECTIVE
  To evaluate the acute and sub-chronic toxicities of Tri-sa-maw recipe extract in rats.
  
  
  METHODS
  In the present study of acute toxicity, a single oral dose 5,000 mg/kg of Tri-sa-maw recipe extract was administered to rats. Sub-chronic toxicity was studied by the daily oral administration ofthe extract at the doses of 600, 1,200 and 2,400 mg/kg body weight for consecutive 90 days.
  
  
  RESULTS
  Tri-sa-maw recipe extract at the dose of 5,000 mg/kg showed no signs of differences as compared to the control rat. No abnormalities were found in the sub-chronic toxicity study; none of the parametersfor body and organ weights, hematol- ogy, blood chemistry, necropsy, and histopathology showed any differences between the control and all treatment groups.
  
  
  CONCLUSIONS
  Tri-sa-maw recipe extract did not significantly cause acute toxicity or sub-chronic toxicity in rats.
authors.forename: Urarat
authors.initials: U
authors.lastname: Nanna
authors.forename: Kanjana
authors.initials: K
authors.lastname: Jaijoy
authors.forename: Nirush
authors.initials: N
authors.lastname: Lertprasertsuke
authors.forename: Noppamas
authors.initials: N
authors.lastname: Soonthornchareonnon
authors.forename: Seewaboon
authors.initials: S
authors.lastname: Sireeratawong
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D005260:Female; D005638:Fruit / Q000737:chemistry; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029766:Terminalia / Q000737:chemistry*; D023382:Toxicity Tests, Acute; D059550:Toxicity Tests, Subchronic
chemical_list: D010936:Plant Extracts