title: Overexpression of CD55 from Barrett's esophagus is associated with esophageal adenocarcinoma risk.
abstract: OBJECTIVE
  Although several molecular biomarkers for esophageal adenocarcinoma (EAC) have been shown to be useful disease indicators, none has been established as a reliable indicator for risk of EAC or have progressed to routine use. The aim was to identify biomarkers of high risk for EAC in patients with Barrett's esophagus (BE).
  
  
  METHODS
  Following endoscopic observation by magnified endoscopy with narrow band imaging (ME-NBI), brushing was followed by obtaining biopsy samples from columnar-lined esophagus (CLE) and from EAC lesions of EAC patients, and from age- and sex-matched non-EAC controls with BE. Total RNA was extracted for microarray analysis using Affymetrix GeneChip Human Genome U133 plus 2.0 Array. Real-time-PCR analysis of identified candidate genes was used to confirm the results.
  
  
  RESULTS
  Overall, 9 EAC patients and 50 patients with BE were studied. Seventy-nine candidate genes were identified by microarray analysis based on a proportional hazards model (P < 0.005). Six genes exhibited significantly differential expressions in both BE and cancer lesions of the EAC group compared to BE of the controls. In the brushing samples, median CD55 relative expression levels in cancer lesions were highest and decreased in BE of EAC group and BE of the controls, in that order (P < 0.001).
  
  
  CONCLUSIONS
  Over expression of CD55 in brushing samples taken from BE may be associated with the risk of EAC.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Murao
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Shiotani
authors.affiliation: Department of Genome Biology, Kinki University Faculty of Medicine, Osaka, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Fujita
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Yamanaka
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Japan.
authors.forename: Tomoari
authors.initials: T
authors.lastname: Kamada
authors.affiliation: Department of Clinical Pathology and Laboratory Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Manabe
authors.affiliation: Department of Clinical Pathology and Laboratory Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Hata
authors.affiliation: Department of Genome Biology, Kinki University Faculty of Medicine, Osaka, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Nishio
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000368:Aged; D001471:Barrett Esophagus / Q000235:genetics*; D001706:Biopsy / Q000379:methods; D018958:CD55 Antigens / Q000032:analysis / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D005819:Genetic Markers; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D012306:Risk
chemical_list: D018958:CD55 Antigens; D005819:Genetic Markers; D012313:RNA
keywords: brushing; magnified endoscopy; microarray; narrow band imaging