title: Size measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment.
abstract: In this work, techniques for monitoring the intramolecular transglutaminase cross-links of pea proteins, based on protein size determination, were developed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of transglutaminase-treated low concentration (0.01% w/w) pea albumin samples, compared to the untreated one (control), showed a higher electrophoretic migration of the major albumin fraction band (26 kDa), reflecting a decrease in protein size. This protein size decrease was confirmed, after DEAE column purification, by dynamic light scattering (DLS) where the hydrodynamic radius of treated samples appears to be reduced compared to the control one.
authors.affiliation: UMR PAM (Procédés Alimentaires et Microbiologiques) AgroSupDijon/Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France; Department of Food Technologies, URDI, Algiers 16000, Algeria.
authors.forename: Attaf
authors.initials: A
authors.lastname: Djoullah
authors.affiliation: UMR PAM (Procédés Alimentaires et Microbiologiques) AgroSupDijon/Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France; Department of Food Technologies, URDI, Algiers 16000, Algeria.
authors.forename: Ghali
authors.initials: G
authors.lastname: Krechiche
authors.affiliation: UMR PAM (Procédés Alimentaires et Microbiologiques) AgroSupDijon/Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Husson
authors.affiliation: UMR PAM (Procédés Alimentaires et Microbiologiques) AgroSupDijon/Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France. Electronic address: remi.saurel@agrosupdijon.fr.
authors.forename: Rémi
authors.initials: R
authors.lastname: Saurel
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000067493:Dynamic Light Scattering / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D018532:Pisum sativum / Q000737:chemistry*; D011503:Transglutaminases / Q000737:chemistry*
chemical_list: D000418:Albumins; D011503:Transglutaminases
keywords: Dynamic light scattering; Electrophoresis; Intramolecular crosslinks; Microbial transglutaminase; Pea albumin