title: Duddingtonia flagrans in the control of gastrointestinal nematodes of horses.
abstract: Horses can harbor a large amount of parasites that may cause serious clinical signs even death. The aim of this study was to evaluate the predatory activity of the fungus Duddingtonia flagrans against infective larvae (L3) of gastrointestinal nematodes of horses in fecal culture. The experimental design was completely randomized with three treated groups (G1, G2 and G3) and one control (CG), using eight animals/group. The treated animals received G1: 1.5 × 10(5); G2: 3 × 10(5) and G3: 6 × 10(5) chlamydospores of D. flagrans/kg body weight during 21 days. The fungi preparation was given at every other three-day interval. Faecal samples were collected during 30 days, on the same interval, to perform the fecal egg counts (EPG) and fecal culture for each horse. All groups demonstrated similar results for the EPG (P > 0.05) counts. D. flagrans significantly reduced (P < 0.05) the number of infective larvae after 72 h-interval between treatments. The G2 and G3 promoted higher results (P < 0.05) of L3 reduction compared to the CG. The biological control with the predacious fungi D. flagrans is still a promising free-living parasite regulator alternative to be use in livestock.
authors.affiliation: Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil. Electronic address: deiabuzatti@gmail.com.
authors.forename: Andréia
authors.initials: A
authors.lastname: Buzatti
authors.affiliation: State University of North Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Clóvis
authors.initials: C
authors.lastname: de Paula Santos
authors.affiliation: Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Maria Angela Machado
authors.initials: MA
authors.lastname: Fernandes
authors.affiliation: Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Ursula Yaeko
authors.initials: UY
authors.lastname: Yoshitani
authors.affiliation: Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Lew Kan
authors.initials: LK
authors.lastname: Sprenger
authors.affiliation: Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Carolina Dallagassa
authors.initials: CD
authors.lastname: dos Santos
authors.affiliation: Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil; National Institute of Science and Technology - Livestock, Belo Horizonte, Brazil.
authors.forename: Marcelo Beltrão
authors.initials: MB
authors.lastname: Molento
mesh_terms: D000818:Animals; D059586:Duddingtonia / Q000502:physiology*; D005243:Feces / Q000469:parasitology; D005260:Female; D006734:Horse Diseases / Q000469:parasitology / Q000628:therapy*; D006736:Horses; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology / Q000628:therapy / Q000662:veterinary*; D007814:Larva / Q000502:physiology; D008297:Male; D009349:Nematode Infections / Q000469:parasitology / Q000628:therapy / Q000662:veterinary*; D010270:Parasite Egg Count / Q000662:veterinary; D010572:Pest Control, Biological / Q000379:methods*; D013997:Time Factors
keywords: Endoparasites; Fecal culture; Fungus; Infective larvae; Pasture