title: Speciation of As(III) and As(V) in fruit juices by dispersive liquid-liquid microextraction and hydride generation-atomic fluorescence spectrometry.
abstract: A sensitive method was developed to speciate and quantify As(III) and As(V) in fruit juices. At pH 3.0, As(III) and ammonium pyrrolidine dithiocarbamate (APDC) formed a complex, which was extracted into CCl4 by dispersive liquid-liquid microextraction (DLLME) and subsequently quantified by hydride generation-atomic fluorescence spectrometry (HG-AFS). After As(V) was reduced by thiosulphate at pH 1.7-1.8, total inorganic arsenic (iAs) was determined following the same protocol and As(V) was calculated from the difference. Interference from methylarsonic acid (MMA) was managed at <10% by controlling the pH of the reduction reaction. This procedure achieved 1.2 μg L(-1) limit of detection (3σ) and 92-102% recovery at 10 μg L(-1), and is applicable to most fruit juices except certain pear juice that may contain considerable MMA.
authors.affiliation: Department of Chemical Engineering, Meizhouwan Vocational Technology College, Putian, Fujian 351254, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Lai
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. Electronic address: guoying.chen@ars.usda.gov.
authors.forename: Guoying
authors.initials: G
authors.lastname: Chen
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Tuanwei
authors.initials: T
authors.lastname: Chen
mesh_terms: D001151:Arsenic / Q000737:chemistry*; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D059627:Liquid Phase Microextraction / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001151:Arsenic
keywords: Atomic fluorescence spectrometry; Dispersive liquid–liquid microextraction; Fruit juice; Hydride generation; Inorganic arsenic; Speciation