title: Evaluation of dense collagen matrices as medicated wound dressing for the treatment of cutaneous chronic wounds.
abstract: Cutaneous chronic wounds are characterized by an impaired wound healing which may lead to infection and amputation. When current treatments are not effective enough, the application of wound dressings is required. To date, no ideal biomaterial is available. In this study, highly dense collagen matrices have been evaluated as novel medicated wound dressings for the treatment of chronic wounds. For this purpose, the structure, mechanical properties, swelling ability and in vivo stability of matrices concentrated from 5 to 40 mg mL(-1) were tested. The matrix stiffness increased with the collagen concentration and was associated with the fibril density and thickness. Increased collagen concentration also enhanced the material resistance against accelerated digestion by collagenase. After subcutaneous implantation in rats, dense collagen matrices exhibited high stability without any degradation after 15 days. The absence of macrophages and neutrophils evidenced their biocompatibility. Subsequently, dense matrices at 40 mg mL(-1) were evaluated as drug delivery system for ampicillin release. More concentrated matrices exhibited the best swelling abilities and could absorb 20 times their dry weight in water, allowing for an efficient antibiotic loading from their dried form. They released efficient doses of antibiotics that inhibited the bacterial growth of Staphylococcus Aureus over 3 days. In parallel, they show no cytotoxicity towards human fibroblasts. These results show that dense collagen matrices are promising materials to develop medicated wound dressings for the treatment of chronic wounds.
authors.affiliation: University Pierre and Marie Curie, Ecole Pratique des Hautes Etudes, CNRS - UMR 7574, Condensed Matter Chemistry Laboratory, Batiment F, 4 place Jussieu, 75005 Paris, France. christophe.helary@upmc.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Helary
authors.forename: Aicha
authors.initials: A
authors.lastname: Abed
authors.forename: Gervaise
authors.initials: G
authors.lastname: Mosser
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.forename: Thibaud
authors.initials: T
authors.lastname: Coradin
authors.forename: Marie Madeleine
authors.initials: MM
authors.lastname: Giraud-Guille
authors.forename: Anne
authors.initials: A
authors.lastname: Meddahi-Pellé
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001684:Biological Dressings; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D017364:Collagenases / Q000737:chemistry* / Q000494:pharmacology*; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D051381:Rats; D012871:Skin Diseases / Q000473:pathology*; D017695:Soft Tissue Injuries / Q000473:pathology*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003094:Collagen; D017364:Collagenases