title: Guanacastane-type diterpenoids from the insect-associated fungus Verticillium dahliae.
abstract: Four new guanacastane-type diterpenoids, namely dahlianes A (1), B (2), C (3), and D (4), were isolated from cultures of Verticillium dahliae. Their structures were elucidated on the basis of extensive spectroscopic data analysis. Their absolute configurations were determined by a combination of Mo2(OAc)4-induced electronic circular dichroism experiment and Mosher ester method. In cytotoxicity evaluation against human tumor cell lines, compounds 2 and 3 exhibited significant cytotoxicities against MCF-7 cell lines with IC50 values of 3.35 and 4.72 μM, respectively.
authors.affiliation: a State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Fa-Biao
authors.initials: FB
authors.lastname: Wu
authors.affiliation: a State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Tian-Xiao
authors.initials: TX
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Yang
authors.affiliation: a State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Ling-Yi
authors.initials: LY
authors.lastname: Kong
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D007313:Insecta / Q000382:microbiology; D061986:MCF-7 Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D029645:Thymelaeaceae / Q000382:microbiology; D020172:Verticillium / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; D004317:Doxorubicin
keywords: Verticillium dahliae; cytotoxicity; guanacastane-type diterpenoid; insect-associated fungus