title: Complete genome sequence of Ostreid herpesvirus-1 associated with mortalities of Scapharca broughtonii broodstocks.
abstract: BACKGROUND
  Ostreid herpesvirus-1 (OsHV-1) is the major bivalve pathogen associated with severe mortality events in a wide host range. In the early summer of 2012 and 2013, mass mortalities of blood clam (Scapharca broughtonii) broodstocks associated with a newly described variant of OsHV-1 (OsHV-1-SB) were reported.
  
  
  METHODS
  In this study, the complete genome sequence of the newly described variant was determined through the primer walking approach, and compared with those of the other two OsHV-1 variants.
  
  
  RESULTS
  OsHV-1-SB genome was found to contain 199, 354 bp nucleotides with 38.5% G/C content, which is highly similar to those of acute viral necrosis virus (AVNV) and OsHV-1 reference type. A total of 123 open reading frames (ORFs) putatively encoding functional proteins were identified; eight of which were duplicated in the major repeat elements of the genome. The genomic organization of OsHV-1-SB could be represented as TRL-UL-IRL-IRS-US-TRS, which is different from that of OsHV-1 reference type and AVNV due to the deletion of a unique region (X, 1.5Kb) between IRL and IRS. The DNA sequence of OsHV-1-SB is 95.2% and 97.3% identical to that of OsHV-1 reference type and AVNV respectively. On the basis of nucleotide sequences of 32 ORFs in OsHV-1-SB and the other nine OsHV-1 variants, results from phylogenetic analysis also demonstrated that OsHV-1-SB is most closely related to AVNV.
  
  
  CONCLUSIONS
  The determination of the genome of OsHV-1 with distinguished epidemiological features will aid in our better understanding of OsHV-1 diversity, and facilitate further research on the origin, evolution, and epidemiology of the virus.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China. xjy_junyang@126.com.|Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China. xjy_junyang@126.com.
authors.forename: Junyang
authors.initials: J
authors.lastname: Xia
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China. baicm@ysfri.ac.cn.|Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, China. baicm@ysfri.ac.cn.
authors.forename: Changming
authors.initials: C
authors.lastname: Bai
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China. wangcm@ysfri.ac.cn.|Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, China. wangcm@ysfri.ac.cn.
authors.forename: Chongming
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China. songxl@ysfri.ac.cn.|Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, China. songxl@ysfri.ac.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Song
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China. huangjie@ysfri.ac.cn.|Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, China. huangjie@ysfri.ac.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D023061:Gene Order; D016679:Genome, Viral; D006564:Herpesviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D049894:Scapharca / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins