title: A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis.
abstract: BACKGROUND
  Lipids play different important roles in central nervous system so that dysregulation of lipid pathways has been implicated in a growing number of neurodegenerative disorders including multiple sclerosis (MS). MS is the most prevalent autoimmune disorder of the central nervous system, with neurological symptoms caused by inflammation and demyelination. In this study, a lipidomic analysis was performed for the rapid profile of CD4(+) T lymphocytes from MS patient and control samples in an untargeted approach.
  
  
  METHODS
  A matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry based approach was used for the analysis of lipid extracts using 9-aminoacridine as matrix. Lipids were analyzed in negative mode and selected species fragmented using MALDI tandem mass spectrometry for their structural assignments.
  
  
  RESULTS
  The analysis reveals some modifications in the phospholipid pattern of MS CD4(+) T lymphocytes with respect to healthy controls with a significant increase of cardiolipin species in MS samples.
  
  
  CONCLUSIONS
  These results demonstrate the feasibility of a MALDI-TOF approach for the analysis of CD4(+) lipid extracts and suggest how alterations in the lipid metabolism characterized lymphocytes of MS patients.
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni, Lecce, Italy. danielevergara@libero.it.|Laboratory of Clinical Proteomic, "Giovanni Paolo II" Hospital, ASL-Lecce, Piazzetta F. Muratore, Lecce, Italy. danielevergara@libero.it.
authors.forename: Daniele
authors.initials: D
authors.lastname: Vergara
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, Research Centre on Aging (Ce.S.I), "G. d'Annunzio" University Foundation, Chieti-Pescara, Italy. michdalessandro@libero.it.
authors.forename: Michele
authors.initials: M
authors.lastname: D'Alessandro
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni, Lecce, Italy. antonia.rizzello@unisalento.it.|Laboratory of Clinical Proteomic, "Giovanni Paolo II" Hospital, ASL-Lecce, Piazzetta F. Muratore, Lecce, Italy. antonia.rizzello@unisalento.it.
authors.forename: Antonia
authors.initials: A
authors.lastname: Rizzello
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni, Lecce, Italy. lidia.dericcardis@unisalento.it.
authors.forename: Lidia
authors.initials: L
authors.lastname: De Riccardis
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni, Lecce, Italy. paola.lunetti@unisalento.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Lunetti
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, Research Centre on Aging (Ce.S.I), "G. d'Annunzio" University Foundation, Chieti-Pescara, Italy. p.delboccio@unich.it.
authors.forename: Piero
authors.initials: P
authors.lastname: Del Boccio
authors.affiliation: Department of Neurology, "Vito Fazzi" Hospital, ASL-Lecce, Piazzetta F. Muratore, Lecce, Italy. francescaderob@libero.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: De Robertis
authors.affiliation: Department of Neurology, "Vito Fazzi" Hospital, ASL-Lecce, Piazzetta F. Muratore, Lecce, Italy. trianni.giorgio@libero.it.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Trianni
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni, Lecce, Italy. michele.maffia@unisalento.it.|Laboratory of Clinical Proteomic, "Giovanni Paolo II" Hospital, ASL-Lecce, Piazzetta F. Muratore, Lecce, Italy. michele.maffia@unisalento.it.
authors.forename: Michele
authors.initials: M
authors.lastname: Maffia
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni, Lecce, Italy. anna.giudetti@unisalento.it.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Giudetti
mesh_terms: D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D016002:Discriminant Analysis; D005227:Fatty Acids / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000378:metabolism*; D015999:Multivariate Analysis; D010743:Phospholipids / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055815:Young Adult
chemical_list: D002308:Cardiolipins; D005227:Fatty Acids; D010743:Phospholipids