title: Identification and characterization of cytosolic fructose-1,6-bisphosphatase in Euglena gracilis.
abstract: Euglena gracilis has the ability to accumulate a storage polysaccharide, a β-1,3-glucan known as paramylon, under aerobic conditions. Under anaerobic conditions, E. gracilis cells degrade paramylon and synthesize wax esters. Cytosolic fructose-1,6-bisphosphatase (FBPase) appears to be a key enzyme in gluconeogenesis and position branch point of carbon partitioning between paramylon and wax ester biosynthesis. We herein identified and characterized cytosolic FBPase from E. gracilis. The Km and Vmax values of EgFBPaseIII were 16.5 ± 1.6 μM and 30.4 ± 7.2 μmol min(-1) mg protein(-1), respectively. The activity of EgFBPaseIII was not regulated by AMP or reversible redox modulation. No significant differences were observed in the production of paramylon in transiently suppressed EgFBPaseIII gene expression cells by RNAi (KD-EgFBPaseIII); nevertheless, FBPase activity was markedly decreased in KD-EgFBPaseIII cells. On the other hand, the growth of KD-EgFBPaseIII cells was slightly higher than that of control cells.
authors.affiliation: a Faculty of Agriculture, Department of Advanced Bioscience , Kinki University , Nara , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Chiyoda-ku , Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: a Faculty of Agriculture, Department of Advanced Bioscience , Kinki University , Nara , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Chiyoda-ku , Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Kimura
authors.affiliation: a Faculty of Agriculture, Department of Advanced Bioscience , Kinki University , Nara , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Chiyoda-ku , Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Sakuyama
authors.affiliation: a Faculty of Agriculture, Department of Advanced Bioscience , Kinki University , Nara , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Chiyoda-ku , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tamoi
authors.affiliation: b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Chiyoda-ku , Japan.|c Faculty of Life and Environmental Science , Shimane University , Matsue , Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: a Faculty of Agriculture, Department of Advanced Bioscience , Kinki University , Nara , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Chiyoda-ku , Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shigeoka
mesh_terms: D000595:Amino Acid Sequence; D018533:Biomass; D003600:Cytosol / Q000201:enzymology*; D005056:Euglena gracilis / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D006597:Fructose-Bisphosphatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005936:Glucans / Q000096:biosynthesis; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D005936:Glucans; D011994:Recombinant Proteins; C003045:paramylon; D006597:Fructose-Bisphosphatase
keywords: Euglena gracilis; cytosol; fructose-1,6-bisphosphatase; paramylon