title: A Role of sFlt-1 in Oxidative Stress and Apoptosis in Human and Mouse Pre-Eclamptic Trophoblasts.
abstract: Pre-eclampsia (PE) is a hypertensive disorder that occurs during pregnancy, and is a multifactorial disease. The antiangiogenic factor, soluble fms-like tyrosine kinase 1 (sFlt-1), has been reported to be important in the pathogenesis of PE, but the mechanism of its involvement remains unknown. To test the effects of sFlt-1 on pregnancy, we injected pregnant mice with exogenous mouse sFlt-1. After 18 days of gestation, higher blood pressure, proteinuria, and histological differences were observed compared with controls. Mitochondrial swelling inside the trophoblast cells in the placenta of sFlt-1-treated pregnant mice was observed by electron microscopy, which suggested a role of sFlt-1 in oxidative stress in trophoblasts in PE. Furthermore, apoptosis markers were upregulated in sFlt-1-treated mice. In conclusion, sFlt-1 appears to play a role in oxidative stress, which promotes apoptosis of trophoblasts. This may be an important mechanism in the development of PE.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Ziyan
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Yuhuangding Hospital of Yantai, Yantai, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zuo
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Shi Yun
authors.initials: SY
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Biochemistry Chemistry and Molecular Biology, Nanjing Medical University, Nanjing, China sunlizhou@njmu.edu.cn.
authors.forename: Lizhou
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D005260:Female; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008933:Mitochondrial Swelling / Q000187:drug effects; D018384:Oxidative Stress / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics* / Q000473:pathology; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000378:metabolism; D014327:Trophoblasts / Q000473:pathology*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics*
chemical_list: D008315:Malondialdehyde; D013482:Superoxide Dismutase; C501162:FLT1 protein, human; C501163:Flt1 protein, mouse; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: apoptosis; mice; oxidative stress; pre-eclampsia; sFlt-1