title: Characterization of Escherichia coli-Producing Extended-Spectrum β-Lactamase (ESBL) Isolated from Chicken Slaughterhouses in South Korea.
abstract: In South Korea, few reports have indicated the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in food-producing animals, particularly in poultry slaughterhouses. In this study, we investigated the occurrence and antibiotic resistance of ESBL-producing E. coli from whole chicken carcasses (n=156) and fecal samples (n=39) of chickens obtained from 2 slaughterhouses. Each sample enriched in buffered peptone water was cultured on MacConkey agar with 2 mg/L cefotaxime and ESBL agar. ESBL production and antibiotic susceptibility were determined using the Trek Diagnostics system. The ESBL genotypes were determined by polymerase chain reaction (PCR) using the bla(SHV), bla(TEM), and bla(CTX-M) gene sequences. Subtyping using a repetitive sequence-based PCR system (DiversiLab™) and multilocus sequence typing (MLST) were used to assess the interspecific biodiversity of isolates. Sixty-two ESBL-producing E. coli isolates were obtained from 156 samples (39.7%). No bla(SHV) genes were detected in any of the isolates, whereas all contained the bla(TEM) gene. Twenty-five strains (40.3%) harbored the CTX-M group 1 gene. The most prevalent MLST sequence type (ST) was ST 93 (14.5%), followed by ST 117 (9.7%) and ST 2303 (8.1%). This study reveals a high occurrence and β-lactams resistance rate of E. coli in fecal samples and whole chickens collected from slaughterhouses in South Korea.
authors.affiliation: 1 KU Center for Food Safety, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lim
authors.affiliation: 1 KU Center for Food Safety, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Da-Som
authors.initials: DS
authors.lastname: Choi
authors.affiliation: 2 Ministry of Food and Drug Safety, Food and Drug Administration , Cheongwon, Korea.
authors.forename: Young-Jo
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: 3 Department of Animal Science and Technology, Konkuk University , Seoul, Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: 1 KU Center for Food Safety, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: 2 Ministry of Food and Drug Safety, Food and Drug Administration , Cheongwon, Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Park
authors.affiliation: 4 Animal and Plant Quarantine Agency , Anyang, Gyeonggi, Korea.
authors.forename: Jin-San
authors.initials: JS
authors.lastname: Moon
authors.affiliation: 4 Animal and Plant Quarantine Agency , Anyang, Gyeonggi, Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Wee
authors.affiliation: 1 KU Center for Food Safety, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000003:Abattoirs; D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology*; D005243:Feces / Q000201:enzymology; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D018440:beta-Lactam Resistance / Q000235:genetics / Q000276:immunology; D001618:beta-Lactamases / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001618:beta-Lactamases