title: Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media.
abstract: We evaluated the isolation and quantitation of Clostridium difficile from aqueous and fecal samples utilizing ChromID CDIF and cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate media. Growth was similar between the two. ChromID CDIF provided enhanced isolation and required no ethanol pretreatment to inhibit normal flora. ChromID CDIF also improved turn-around time, requiring only 24 hours' incubation.
authors.affiliation: Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT, USA.
authors.forename: Seth T
authors.initials: ST
authors.lastname: Housman
authors.affiliation: Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT, USA.
authors.forename: Mary Anne
authors.initials: MA
authors.lastname: Banevicius
authors.affiliation: Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT, USA.
authors.forename: Lucinda M
authors.initials: LM
authors.lastname: Lamb
authors.affiliation: Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT, USA; Division of Infectious Diseases, Hartford Hospital, Hartford, CT, USA. Electronic address: david.nicolau@hhchealth.org.
authors.forename: David P
authors.initials: DP
authors.lastname: Nicolau
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002440:Cefoxitin / Q000494:pharmacology; D002863:Chromogenic Compounds / Q000494:pharmacology; D016360:Clostridioides difficile / Q000254:growth & development* / Q000302:isolation & purification*; D003470:Culture Media; D003523:Cycloserine / Q000494:pharmacology; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D005632:Fructose / Q000378:metabolism; D006801:Humans
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D005632:Fructose; D002440:Cefoxitin; D003523:Cycloserine
keywords: Chromogenic agar; Clostridium difficile; Cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate