title: BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape.
abstract: Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties.
authors.affiliation: College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China. chenquan0616@126.com.|Xiaozhou Agricultural Service Center in Wanzhou District, Chongqing 404089, China. chenquan0616@126.com.
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China. wantjay@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China. mingshenghou@mail.hzau.edu.cn.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Hou
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biologyand Genetic Breeding of Oil Crops, the Ministry of Agriculture, Wuhan 430062, China. liusy@oilcrops.cn.
authors.forename: Shengyi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biologyand Genetic Breeding of Oil Crops, the Ministry of Agriculture, Wuhan 430062, China. huangjy@oilcrops.cn.
authors.forename: Junyan
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China. caili@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D017799:Cucumovirus / Q000276:immunology* / Q000502:physiology; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017800:Potyvirus / Q000276:immunology* / Q000502:physiology; D017898:Tobamovirus / Q000276:immunology* / Q000502:physiology; D019562:Viral Load
chemical_list: D010940:Plant Proteins
keywords: BnSGS3; breeding strategy; oilseed rape; virus; virus accumulation