title: C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer.
abstract: C14ORF166 (chromosome 14 open reading frame 166) is a transcriptional repressor related to the regulation of centrosome architecture. However, the role of C14ORF166 in the development and progression of cancer remains largely unknown. The aim of this study was to investigate the expression and clinicopathological significance of C14ORF166 in cervical cancer. C14ORF166 expression was analyzed using quantitative real-time PCR (RT-PCR) and Western blotting in cervical cancer cell lines and eight paired cervical cancer samples and the adjacent normal tissues. Immunohistochemistry was used to analyze C14ORF166 protein expression in 148 clinicopathologically characterized cervical cancer specimens. Statistical analyses were performed to evaluate the relationship between the expression of C14ORF166 and clinicopathologic features and prognosis. C14ORF166 mRNA and protein expression were significantly upregulated in cervical cancer cell lines and tissue samples (P < 0.05). Immunohistochemical analysis revealed a high expression of C14ORF166 was observed in 39.9 % (59/148) of the cervical cancer specimens; the remaining samples expressed low levels or did not express any detectable C14ORF166. The chi-square test indicated that high-level expression of C14ORF166 was significantly associated with International Federation of Gynecology and Obstetrics (FIGO) stage (P < 0.001), vital status (P = 0.026), tumor size (P = 0.034), serum squamous cell carcinoma antigen level (SCC-Ag; P = 0.035), and pelvic lymph node metastasis (P < 0.001). Patients with highly expressed C14ORF166 showed a tendency to receive postoperative chemotherapy (P = 0.005) and postoperative radiation (P = 0.008). Furthermore, high C14ORF166 expression was associated with poorer overall survival compared to low C14ORF166 expression, and C14ORF166 was a significant prognostic factor in univariate and multivariate analysis (P < 0.05). High C14ORF166 expression had prognostic value for poor outcome in cervical cancer. C14ORF166 may represent a biomarker of pelvic lymph node metastasis and enable the identification of high-risk patients along with selection of appropriate treatment strategies.
authors.affiliation: Department of Gynecologic Oncology; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Cancer Center, Sun Yat-Sen University, No. 651, Dongfeng Road East, Guangzhou, 510060, People's Republic of China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tian He Road, GuangZhou, 510630, People's Republic of China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Ou
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, People's Republic of China.
authors.forename: Fangyong
authors.initials: F
authors.lastname: Lei
authors.affiliation: Department of Urology, Wuhan Union Hospital of Huazhong University of Science and Technology, No. 1277, Han Kou Jie Fang Road, Wuhan, 430022, People's Republic of China.
authors.forename: Teng
authors.initials: T
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Gynecologic Oncology; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Cancer Center, Sun Yat-Sen University, No. 651, Dongfeng Road East, Guangzhou, 510060, People's Republic of China.
authors.forename: Chunhao
authors.initials: C
authors.lastname: Niu
authors.affiliation: Department of Gynecology, Women and Children Hospital of Guangdong Province, No. 13, Guang Yuan Road, Guangzhou, 510060, People's Republic of China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Gynecologic Oncology; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Cancer Center, Sun Yat-Sen University, No. 651, Dongfeng Road East, Guangzhou, 510060, People's Republic of China. zhangyn@sysucc.org.cn.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016366:Open Reading Frames; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D015534:Trans-Activators / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C435504:RTRAF protein, human; D015534:Trans-Activators
keywords: Biomarker; C14ORF166; Cervical cancer; Lymph node metastasis; Prognosis