title: A novel fluorescence polarization assay for determination of penicillin G in milk.
abstract: Detection of penicillin G in milk is of interest because of the wide use of this antibiotic in livestock. Current analytical methods used to quantify the penicillin G in milk are based on HPLC, mass spectrometry and electrophoresis. These methodologies are time-consuming and require trained personnel. In addition, it is not easy to envisage the development of a portable device for in situ analyses based on these methods. We present a novel sensing approach for detecting the presence of penicillin G in milk. The proposed method is based penicillin G conjugate labeled with red-emitting dye with properly produced anti-penicillin G antibodies. The results obtained suggest our method could be applied directly in milk without interference from other substances. The limit of detection of the method was 1.0 nmol/L, which is much less than the required MRL in EU regulations (12.0 nmol/L).
authors.affiliation: Laboratory for Molecular Sensing, Institute of Protein Biochemistry CNR, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Pennacchio
authors.affiliation: Laboratory for Molecular Sensing, Institute of Protein Biochemistry CNR, Via P. Castellino 111, 80131 Naples, Italy; Institute of Food Science, CNR, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Varriale
authors.affiliation: Laboratory for Molecular Sensing, Institute of Protein Biochemistry CNR, Via P. Castellino 111, 80131 Naples, Italy; Institute of Food Science, CNR, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Scala
authors.affiliation: Laboratory for Molecular Sensing, Institute of Protein Biochemistry CNR, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Vincenzo Manuel
authors.initials: VM
authors.lastname: Marzullo
authors.affiliation: Laboratory for Molecular Sensing, Institute of Protein Biochemistry CNR, Via P. Castellino 111, 80131 Naples, Italy; Institute of Food Science, CNR, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.affiliation: Institute of Food Science, CNR, Via Roma 64, 83100 Avellino, Italy. Electronic address: sabato.dauria@cnr.it.
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005454:Fluorescence Polarization; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D010400:Penicillin G / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D010400:Penicillin G
keywords: Antibody; Biosensors; Fluorescence; Milk; Penicillin G; Penicillin G (PubChem CID 5904)