title: Development of an immunochromatography assay kit for rapid detection of ranavirus.
abstract: Ranaviruses are large, double-stranded DNA viruses of the family Iridoviridae and are known to be primary pathogens in frogs, fish and other amphibians. These viruses have been shown to be highly adaptable and have the ability to cross species barriers, making them a potent threat to global biodiversity. There is therefore, a need for rapid and efficient diagnostic methods to control the spread of these viruses. To address this, monoclonal antibodies (MAbs) were developed against ranavirus strain FV-3 (standard frog virus 3) to detect the major capsid protein and FV-3gorf19R related hypothetical protein in both the FV-3 and KRV-1 (Korean ranavirus) strains. The antibodies were then applied on a colloidal gold-immunochromatographic assay (GICA) as a kit for the detection of ranaviruses. The kit was able to detect low concentrations of the virus (10(1)TCID50/ml) and showed analytical specificity when tested against other viral pathogens, including those belonging to the same family. It was possible to detect ranavirus in experimentally infected frogs within 30 min using the kit. The kit described here is expected to be a valuable and informative tool for on-site detection of ranavirus in frog.
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Rim
authors.initials: YR
authors.lastname: Kim
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762-6100, USA.
authors.forename: Seong Bin
authors.initials: SB
authors.lastname: Park
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Fernand F
authors.initials: FF
authors.lastname: Fagutao
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762-6100, USA.
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: In Seok
authors.initials: IS
authors.lastname: Cha
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Kim D
authors.initials: KD
authors.lastname: Thompson
authors.affiliation: Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Adams
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Bayley
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: jungts@gnu.ac.kr.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000663:Amphibians / Q000821:virology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D007497:Iridoviridae; D008807:Mice, Inbred BALB C; D017995:Ranavirus / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
keywords: Colloidal gold-immunochromatography assay (GICA); Detection kit; Frog; Monoclonal antibodies; Ranavirus