title: Regulation of Intermittent Hypoxia on Brain Dopamine in Amphetaminized Rats.
abstract: We investigated intermittent hypoxia (IH) on dopamine (DA) release in rat brain treated with or without amphetamine (AMPH). Rats were divided into four groups including normoxia, IH, AMPH, and AMPH + IH treatments. The cerebrospinal fluid (CSF) was collected and the DA levels were detected by high performance liquid chromatography (HPLC). The plasma prolactin (PRL) concentration was measured by radioimmunoassay (RIA). We found that IH reduced basal DA concentration in media prefrontal cortex (mPFC), but increased that in striatum, where DA level was also increased in rats treated with AMPH or AMPH + IH. Angiotensin II (Ang II) increased the DA release in mPFC and striatum and this effect was enhanced in AMPH + IH group. The stimulatory effect of IH on plasma PRL was attenuated in presence of AMPH. Tyrosine hydroxylase (TH) expression was decreased by IH, but increased by AMPH + IH in mPFC. IH or AMPH treatment decreased the expression of vesicular monoamine transporter-2 (VMAT-2) in rat brain. These data suggested that IH altered the DA release and changed the protein expression levels in different parts of rat brain treated with AMPH. IH may play a role in regulating DA metabolism in AMPH addiction.
authors.affiliation: Department of Surgery, Taipei City Hospital, Taipei 10431.|Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Po-Ling
authors.initials: PL
authors.lastname: Yu
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Cheng
authors.affiliation: Medical Center of Aging Research, China Medical University Hospital, Taichung 40402, Taiwan, Republic of China.
authors.forename: Jou-Chun
authors.initials: JC
authors.lastname: Chou
authors.affiliation: Department of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Wynn H T
authors.initials: WH
authors.lastname: Pan
authors.affiliation: Department of Physiology and Pharmacology, College of Medicine, Chang-Gung University, Taoyuan 33333, Taiwan, Republic of China.
authors.forename: Shyi-Wu
authors.initials: SW
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.|Medical Center of Aging Research, China Medical University Hospital, Taichung 40402, Taiwan, Republic of China.
authors.forename: Paulus S
authors.initials: PS
authors.lastname: Wang
mesh_terms: D000661:Amphetamine / Q000633:toxicity*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D011388:Prolactin / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000804:Angiotensin II; D011388:Prolactin; D000661:Amphetamine; D004298:Dopamine