title: Cancer-targeted near infrared imaging using rare earth ion-doped ceramic nanoparticles.
abstract: The use of near-infrared (NIR) light over 1000 nm (OTN-NIR or second NIR) is advantageous for bioimaging because it enables deep tissue penetration due to low scattering and autofluorescence. In this report, we describe the application of rare earth ion-doped ceramic nanoparticles to cancer-targeted NIR imaging using erbium and ytterbium ion-doped yttrium oxide nanoparticles (YNP) functionalized with streptavidin via bi-functional PEG (SA-YNP). YNP has NIR emission at 1550 nm, with NIR excitation at 980 nm (NIR-NIR imaging). Cancer-specific NIR-NIR imaging was demonstrated using SA-YNP and biotinylated antibodies on cancer cells and human colon cancer tissues. NIR-NIR imaging through porcine meat of 1 cm thickness was also demonstrated, supporting the possible application of deep tissue NIR-NIR bioimaging using YNP as a probe. Our results suggest that non-invasive imaging using YNP has great potential for general application in cancer imaging in living subjects.
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. zako@riken.jp.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Zako
authors.forename: Miya
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hyodo
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kishimoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ito
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kaneko
authors.forename: Kohei
authors.initials: K
authors.lastname: Soga
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002516:Ceramics / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000737:chemistry / Q000628:therapy*; D004871:Erbium / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D008674:Metals, Rare Earth / Q000008:administration & dosage / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D013552:Swine; D015019:Yttrium / Q000737:chemistry*
chemical_list: D008674:Metals, Rare Earth; D015019:Yttrium; D004871:Erbium; D019809:Streptavidin