title: New fluorescence probes for biomolecules.
abstract: Steady state fluorescence measurements have been used for the investigation of interaction between the bovine serum albumin (BSA) and fluorescence probes: 3-hydroxy-2,4- bis[(3-methyl-1,3-benzoxazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ6), 3-hydroxy- 2,4-bis[(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ7) and 3-hydroxy-2,4-bis[(1,3,3-trimethyl-1,3-dihydro-2H-indol-2-ylidene)methyl]cyclobut-2-en-1-one (SQ8). The binding constant between bovine serum albumin and squarine dyes has been determined by using both the Benesi-Hildebrand and Stern-Volmer equations. The negative value of free energy change indicates the existence of a spontaneous complexation process of BSA with squarine dyes.
authors.affiliation: Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. katarzyna.jurek@utp.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jurek
authors.affiliation: Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. nina@utp.edu.pl.
authors.forename: Janina
authors.initials: J
authors.lastname: Kabatc
authors.affiliation: Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. katarzyna.kostrzewska@utp.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kostrzewska
authors.affiliation: Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland. marlena.grabowska@o2.pl.
authors.forename: Marlena
authors.initials: M
authors.lastname: Grabowska
mesh_terms: D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine
keywords: biomolecule determination; bovine serum albumin; fluorescence method; fluorescence probe; squarine dyes