title: Antinociceptive effect of intrathecal amiloride on neuropathic pain in rats.
abstract: The aim of this study was to investigate the antinociception of intrathecal amiloride and examine its effect on the neuropathic pain-induced activation of c-Fos and p-p38 MAPK in the rat spinal dorsal horn (SDH). Sprague-Dawley rats were chronically implanted with intrathecal catheters, and the ability of intrathecal amiloride to alleviate nociceptive behaviours in rats with neuropathic pain was tested. Immunohistochemical techniques were utilized to detect the expression of c-Fos and p-p38 in SDH in the control and amiloride (100μg) groups. Intrathecal amiloride produced dose- and time-dependent antinociception in rats. Additionally, immunohistochemical experiments showed that the expression of c-Fos and p-p38 dramatically decreased in the superficial laminae of the ipsilateral SDH in the 100-μg amiloride group (P<0.01), whereas, there was no statistical significance on the contralateral side, compared with the control group. Intrathecally administered amiloride develops dose- and time-dependent antinociceptive action in rats with neuropathic pain. It most likely reduces spinal neurons and microglia activation via inhibiting c-Fos and p-p38 MAPK in the SDH of rats.
authors.affiliation: Department of Anesthesiology, Cancer Center, Sun Yat-Sen University, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, People's Republic of China.
authors.forename: Peizong
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Cancer Center, Sun Yat-Sen University, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, People's Republic of China.
authors.forename: Handong
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Department of Anesthesiology, Cancer Center, Sun Yat-Sen University, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, 1st Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510405, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of Anesthesiology, Cancer Center, Sun Yat-Sen University, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Cancer Center, Sun Yat-Sen University, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, People's Republic of China. Electronic address: zengwa@mail.sysu.edu.cn.
authors.forename: Weian
authors.initials: W
authors.lastname: Zeng
mesh_terms: D000584:Amiloride / Q000627:therapeutic use*; D000700:Analgesics / Q000627:therapeutic use*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D007278:Injections, Spinal; D008297:Male; D009048:Motor Skills / Q000187:drug effects; D009437:Neuralgia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D010766:Phosphorylation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D066148:Spinal Cord Dorsal Horn / Q000187:drug effects / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000700:Analgesics; D016760:Proto-Oncogene Proteins c-fos; D000584:Amiloride; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Amiloride; Neuropathic pain; c-Fos; p38 MAPK