title: Nonstructural protein Pns12 of rice dwarf virus is a principal regulator for viral replication and infection in its insect vector.
abstract: Plant reoviruses are thought to replicate and assemble within cytoplasmic structures called viroplasms. The molecular mechanisms underling the formation of the viroplasm during infection of rice dwarf virus (RDV), a plant reovirus, in its leafhopper vector cells remain poorly understood. Viral nonstructural protein Pns12 forms viroplasm-like inclusions in the absence of viral infection, suggesting that the viroplasm matrix is basically composed of Pns12. Here, we demonstrated that core capsid protein P3 and nonstructural protein Pns11 were recruited in the viroplasm by direct interaction with Pns12, whereas nonstructural protein Pns6 was recruited through interaction with Pns11. The introduction of dsRNA from Pns12 gene into cultured insect vector cells or intact insect strongly inhibited such viroplasm formation, preventing efficient viral spread in the leafhopper in vitro and in vivo. Thus, nonstructural protein Pns12 of RDV is a principal regulator for viral replication and infection in its insect vector.
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Jia
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors.forename: Qianzhuo
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China. Electronic address: xielh@126.com.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xei
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China. Electronic address: weitaiyun@fafu.edu.cn.
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism; D002478:Cells, Cultured; D007313:Insecta; D025941:Protein Interaction Mapping; D012087:Reoviridae / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins
keywords: Nonstructural protein Pns12; RNA interference; Rice dwarf virus; Viral replication; Viroplasm