title: Accumulation of flavanols and expression of leucoanthocyanidin reductase induced by postharvest UV-C irradiation in grape berry.
abstract: To examine the effect of postharvest ultraviolet C (UV-C) irradiation on flavanol polyphenol accumulation in the grape berry, we investigated total flavanol polyphenol content, the enzyme activity of leucoanthocyanidin reductase (LAR), and transcription of Vv lar1 and Vv lar2 using spectrophotometry, real-time polymerase chain reaction, and western blot analysis in 5-year-old Vitis vinifera L. cv. Cabernet Sauvignon plants. Our results indicated that the accumulation of flavanol polyphenol reached its highest value when exposed to UV-C irradiation for 30 min. Additionally, UV-C irradiation induced the transcription of Vv lar1 and Vv lar2 and the synthesis of LAR1 and LAR2 proteins, resulting in increased accumulation of flavanol polyphenol in the grape berry. Moreover, these effects were associated with the length of time of UV-C irradiation.
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China yuejinwanglab1@163.com.
authors.forename: P F
authors.initials: PF
authors.lastname: Wen
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: W
authors.initials: W
authors.lastname: Ji
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Gao
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Niu
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Xing
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Niu
mesh_terms: D000872:Anthocyanins / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology / Q000254:growth & development / Q000528:radiation effects*; D010088:Oxidoreductases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D059808:Polyphenols / Q000378:metabolism; D014466:Ultraviolet Rays; D027843:Vitis / Q000201:enzymology* / Q000254:growth & development / Q000528:radiation effects*
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D010940:Plant Proteins; D059808:Polyphenols; D010088:Oxidoreductases