title: Supramolecular organization of photosynthetic complexes in membranes of Roseiflexus castenholzii.
abstract: The photosynthetic membranes of the filamentous anoxygenic phototroph Roseiflexus castenholzii have been studied with electron microscopy, atomic force microscopy, and biochemistry. Electron microscopy of the light-harvesting reaction center complex produced a 3D model that aligns with the solved crystal structure of the RC-LH1 from Thermochromatium tepidum with the H subunit removed. Atomic force microscopy of the whole membranes yielded a picture of the supramolecular organization of the major proteins in the photosynthetic electron transport chain. The results point to a loosely packed membrane without accessory antenna proteins or higher order structure.
authors.affiliation: Department of Chemistry, Washington University in St. Louis, 1 Brookings Dr., CB 1134, St. Louis, MO, 63130, USA.
authors.forename: Erica L-W
authors.initials: EL
authors.lastname: Majumder
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Olsen
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
authors.forename: Pu
authors.initials: P
authors.lastname: Qian
authors.affiliation: Department of Chemistry, Washington University in St. Louis, 1 Brookings Dr., CB 1134, St. Louis, MO, 63130, USA.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Collins
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
authors.forename: C Neil
authors.initials: CN
authors.lastname: Hunter
authors.affiliation: Department of Chemistry, Washington University in St. Louis, 1 Brookings Dr., CB 1134, St. Louis, MO, 63130, USA. blankenship@wustl.edu.|Department of Biology, Washington University in St. Louis, 1 Brookings Dr., CB 1134, St. Louis, MO, 63130, USA. blankenship@wustl.edu.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Blankenship
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry*; D041862:Chloroflexi / Q000737:chemistry* / Q000378:metabolism; D002841:Chromatiaceae / Q000737:chemistry; D006418:Heme / Q000032:analysis; D021621:Imaging, Three-Dimensional; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010788:Photosynthesis
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D006418:Heme
keywords: Alternative complex III; Atomic force microscopy; Electron microscopy; Reaction center light-harvesting complex