title: Phospholipase A1-catalyzed hydrolysis of soy phosphatidylcholine to prepare l-α-glycerylphosphorylcholine in organic-aqueous media.
abstract: This study aimed to optimize the preparation of L-α-glycerylphosphorylcholine (l-α-GPC) via phospholipase A1 (Lecitase Ultra)-catalyzed hydrolysis of soy phosphatidylcholine (PC). The reaction was performed in n-hexane-water biphasic media in a stirred batch reactor, and modeling and optimization were conducted using response surface methodology. Optimal conditions to completely hydrolyze PC to L-α-GPC were: temperature, 50 °C; reaction time, 30 h; water content, 69 g/100 g of PC weight; and enzyme loading, 13 g/100 g of PC weight. The optimal n-hexane-to-water ratio in the medium was 5.8:1 (v/v), and 21.3g of PC was treated as the substrate in 100 mL of the medium. L-α-GPC with purity 99.3 g/100 g was obtained from the reaction products after diethyl ether extraction and silica column chromatography. These findings suggest that the use of n-hexane-water media increases the productivity of l-α-GPC compared to the aqueous media used in enzymatic reaction systems in other published studies.
authors.affiliation: Department of Food Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Bang
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea. Electronic address: bhkim@cau.ac.kr.
authors.forename: Byung Hee
authors.initials: BH
authors.lastname: Kim
mesh_terms: D002384:Catalysis; D005997:Glycerylphosphorylcholine / Q000737:chemistry*; D006868:Hydrolysis; D010713:Phosphatidylcholines / Q000737:chemistry*; D054466:Phospholipases A1 / Q000737:chemistry*; D045730:Soy Foods / Q000032:analysis*
chemical_list: D010713:Phosphatidylcholines; D005997:Glycerylphosphorylcholine; D054466:Phospholipases A1
keywords: Acyl migration; Hydrolysis; Phosphatidylcholine; Phospholipase A(1); Response surface methodology; l-α-Glycerylphosphorylcholine