title: Protective Effects of Total Glucosides of Paeony on N-nitrosodiethylamine-induced Hepatocellular Carcinoma in Rats via Down-regulation of Regulatory B Cells.
abstract: Total glucoside of paeony (TGP), extracted from the root of Paeonia Lactiflora, has been known to show anti-inflammatory, anti-oxidative, hepato-protective and immuno-regulatory activities. The aim of this present study was to determine the anti-tumor effect of TGP against N-nitrosodiethylamine (DEN)-induced hepatocellular carcinoma (HCC) in rats, and to find the related mechanisms. Rat HCC model was established by intragastrically administrating with DEN (8 mg/kg). We found the number of tumor nodules and the index of liver and spleen were increased in the model group compared with the normal group, and was significantly decreased by TGP. Additionally, TGP obviously improved the hepatic pathological lesions induced by DEN, and decreased the elevated levels of serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST), alkaline phosphatase (ALP) and alpha fetoprotein (AFP) by DEN. Moreover, TGP decreased the level of B cell-activating factor (BAFF) and the proportion of IL-10-producing regulatory B cells (Bregs), and the decrease of BAFF by TGP is positively correlated to the decrease of IL-10-producing Bregs by TGP. These results suggest that TGP had a good therapeutic action on DEN-induced HCC rats, which might be due to its down-regulation of Bregs through reducing the level of BAFF.
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei , Anhui Province , China.
authors.forename: S S
authors.initials: SS
authors.lastname: Song
authors.forename: P F
authors.initials: PF
authors.lastname: Yuan
authors.forename: P P
authors.initials: PP
authors.lastname: Li
authors.forename: H X
authors.initials: HX
authors.lastname: Wu
authors.forename: W J
authors.initials: WJ
authors.lastname: Ni
authors.forename: J T
authors.initials: JT
authors.lastname: Lu
authors.forename: W
authors.initials: W
authors.lastname: Wei
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D053264:B-Cell Activating Factor / Q000097:blood; D060151:B-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D004052:Diethylnitrosamine; D015536:Down-Regulation; D005960:Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D016753:Interleukin-10 / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008297:Male; D029596:Paeonia; D008517:Phytotherapy; D017207:Rats, Sprague-Dawley
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D053264:B-Cell Activating Factor; D005960:Glucosides; D016753:Interleukin-10; D004052:Diethylnitrosamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase
keywords: B cell-activating factor; N-nitrosodiethylamine; hepatocellular carcinoma; regulatory B cells; total glucosides of paeony