title: The capture proteasome assay (CAPA) to evaluate subtype-specific proteasome inhibitors.
abstract: We recently developed a new assay to measure proteasome activity in vitro (CAPA for capture proteasome assay) [1], based on proteasome capture on an antibody-coated plate. When used with lysates originating from cells expressing either standard proteasome, immunoproteasome or intermediate proteasomes β5i or β1i-β5i, this assay allows the individual monitoring of the chymotrypsin-like, trypsin-like and caspase-like activities of the corresponding proteasome subtypes. The efficiency and specificity of four proteasome inhibitors were studied using the CAPA assay, demonstrating the potential of this assay for the development of subtype-specific proteasome inhibitors.
authors.affiliation: Ludwig Institute for Cancer Research, Avenue Hippocrate 74, UCL 7459, B-1200 Brussels, Belgium ; WELBIO (Walloon Excellence in Life Sciences and Biotechnology), Brussels, Belgium ; de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vigneron
authors.affiliation: Ludwig Institute for Cancer Research, Avenue Hippocrate 74, UCL 7459, B-1200 Brussels, Belgium ; WELBIO (Walloon Excellence in Life Sciences and Biotechnology), Brussels, Belgium ; de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Joanna
authors.initials: J
authors.lastname: Abi Habib
authors.affiliation: Ludwig Institute for Cancer Research, Avenue Hippocrate 74, UCL 7459, B-1200 Brussels, Belgium ; WELBIO (Walloon Excellence in Life Sciences and Biotechnology), Brussels, Belgium ; de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Benoît J
authors.initials: BJ
authors.lastname: Van den Eynde
keywords: Activity assay; Fluorogenic peptides; Proteasome; Proteasome-Glo