title: Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
abstract: Melanomas are aggressive skin tumors characterized by high metastatic potential. Our previous results indicate that Natural Killer (NK) cells may control growth of melanoma. The main defect of blood NK cells was a decreased expression of activating NCR1/NKp46 receptor and a positive correlation of NKp46 expression with disease outcome in stage IV melanoma patients was found. In addition, in stage III melanoma patients, we identified a new subset of mature NK cells in macro-metastatic Lymph nodes (LN). In the present studies, we evaluated the numbers of NK cells infiltrating primary cutaneous melanoma and analyzed immune cell subsets in a series of sentinel lymph nodes (SLN). First, we show that NKp46+ NK cells infiltrate primary cutaneous melanoma. Their numbers were related to age of patients and not to Breslow thickness. Then, a series of patients with tumor-negative or -positive sentinel lymph nodes matched for Breslow thickness of the cutaneous melanoma was constituted. We investigated the distribution of macrophages (CD68), endothelial cells, NK cells, granzyme B positive (GrzB+) cells and CD8+ T cells in the SLN. Negative SLN (SLN-) were characterized by frequent adipose involution and follicular hyperplasia compared to positive SLN (SLN+). High densities of macrophages and endothelial cells (CD34), prominent in SLN+, infiltrate SLN and may reflect a tumor favorable microenvironment. Few but similar numbers of NK and GrzB+ cells were found in SLN- and SLN+: NK cells and GrzB+ cells were not correlated. Numerous CD8+ T cells infiltrated SLN with a trend for higher numbers in SLN-. Moreover, CD8+ T cells and GrzB+ cells correlated in SLN- not in SLN+. We also observed that the numbers of CD8+ T cells negatively correlated with endothelial cells in SLN-. The numbers of NK, GrzB+ or CD8+ T cells had no significant impact on overall survival. However, we found that the 5 year-relapse rate was higher in SLN with higher numbers of NK cells.
authors.affiliation: INSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
authors.forename: Meriem
authors.initials: M
authors.lastname: Messaoudene
authors.affiliation: U1015 INSERM-CIC, Institut Gustave Roussy, Villejuif, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Périer
authors.affiliation: INSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
authors.forename: Giulia
authors.initials: G
authors.lastname: Fregni
authors.affiliation: INSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Neves
authors.affiliation: U1015 INSERM-CIC, Institut Gustave Roussy, Villejuif, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Zitvogel
authors.affiliation: Centre de Recherche des Cordeliers, 15, rue de l'école de Médecine, 75006, Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cremer
authors.affiliation: APHP, Department of Dermatology, Hospital Cochin, University Paris Descartes, Paris, France.
authors.forename: Johan
authors.initials: J
authors.lastname: Chanal
authors.affiliation: Biopathology Department, Institut Curie, Rue d'Ulm, Paris, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Sastre-Garau
authors.affiliation: APHP, Department of Dermatology and Department of Pathology, Hospital Bichat, University Paris Diderot, Hospital Bichat, 75018, Paris, France.
authors.forename: Lydia
authors.initials: L
authors.lastname: Deschamps
authors.affiliation: APHP, Department of Dermatology and Department of Pathology, Hospital Bichat, University Paris Diderot, Hospital Bichat, 75018, Paris, France.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Marinho
authors.affiliation: APHP, Department of Pathology, Hospital Cochin, University Paris Descartes, Paris, France.
authors.forename: Frederique
authors.initials: F
authors.lastname: Larousserie
authors.affiliation: APHP, Department of Dermatology and Department of Pathology, Hospital Bichat, University Paris Diderot, Hospital Bichat, 75018, Paris, France.
authors.forename: Eve
authors.initials: E
authors.lastname: Maubec
authors.affiliation: APHP, Department of Dermatology, Hospital Cochin, University Paris Descartes, Paris, France.
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Avril
authors.affiliation: INSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Caignard
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000943:Antigens, Differentiation / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged; D009367:Neoplasm Staging; D021701:Sentinel Lymph Node Biopsy; D059016:Tumor Microenvironment
chemical_list: D000943:Antigens, Differentiation