title: Bioactive nanoparticles improve calcium handling in failing cardiac myocytes.
abstract: OBJECTIVE
  To evaluate the ability of N-acetylglucosamine (GlcNAc) decorated nanoparticles and their cargo to modulate calcium handling in failing cardiac myocytes (CMs).
  
  
  METHODS
  Primary CMs isolated from normal and failing hearts were treated with GlcNAc nanoparticles in order to assess the ability of the nanoparticles and their cargo to correct dysfunctional calcium handling in failing myocytes.
  
  
  CONCLUSIONS
  GlcNAc particles reduced aberrant calcium release in failing CMs and restored sarcomere function. Additionally, encapsulation of a small calcium-modulating protein, S100A1, in GlcNAc nanoparticles also showed improved calcium regulation. Thus, the development of our bioactive nanoparticle allows for a 'two-hit' treatment, by which the cargo and also the nanoparticle itself can modulate intracellular protein activity.
authors.affiliation: Wallace H Coulter Department of Biomedical Engineering, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Joshua T
authors.initials: JT
authors.lastname: Maxwell
authors.affiliation: Wallace H Coulter Department of Biomedical Engineering, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.
authors.forename: Inthirai
authors.initials: I
authors.lastname: Somasuntharam
authors.affiliation: Wallace H Coulter Department of Biomedical Engineering, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.
authors.forename: Warren D
authors.initials: WD
authors.lastname: Gray
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Shen
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Singer
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Talib
authors.initials: T
authors.lastname: Saafir
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Brian H
authors.initials: BH
authors.lastname: Crawford
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Bioengineering, University of California Berkeley, Berkeley, CA, USA.
authors.forename: Niren
authors.initials: N
authors.lastname: Murthy
authors.affiliation: Wallace H Coulter Department of Biomedical Engineering, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Davis
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, 1648 Pierce Dr NE, Atlanta, GA 30307, USA.|Children's Heart Research & Outcomes (HeRO) Center, Children's Healthcare of Atlanta & Emory University, Atlanta, GA, USA.
authors.forename: Mary B
authors.initials: MB
authors.lastname: Wagner
mesh_terms: D000117:Acetylglucosamine / Q000008:administration & dosage* / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D006333:Heart Failure / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009418:S100 Proteins / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism / Q000473:pathology
chemical_list: D009418:S100 Proteins; C110294:S100A1 protein; D002118:Calcium; D000117:Acetylglucosamine
keywords: GlcNAc; S100A1; biomolecules; calcium handling; cardiac myocytes; encapsulation; heart failure; nanoparticles