title: Genome-wide whole blood microRNAome and transcriptome analyses reveal miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swine.
abstract: To understand the role of miRNAs in regulating genes involved in host response to bacterial infection and shedding of foodborne pathogens, a systematic profiling of miRNAs and mRNAs from the whole blood of pigs upon Salmonella challenge was performed. A total of 62 miRNAs were differentially expressed post infection (false discovery rate <0.1). An integrative analysis of both the differentially expressed miRNAs and mRNAs using sequence-based miRNA target prediction and negative correlation of miRNA-mRNA profiles helped identify miRNA-mRNA networks that may potentially regulate host response to Salmonella infection. From these networks, miR-214 and miR-331-3p were identified as new candidates potentially associated with Salmonella infection. An miRNA seed sequence analysis suggested that these miRNAs regulate several critical immune-related genes including SLC11A1, PIGE-108A11.3 and VAV2. We showed that challenged pigs had reduced miR-214 expression and increased miR-331-3p expression in the whole blood. Furthermore, the expression of the proposed targets of miR-214 (SLC11A1 and PIGE-108A11.3) increased while that of the proposed target of miR-331-3p (VAV2) decreased following challenge (expression changes confirmed by in vitro assays). Based on these observations, we propose potential roles for miR-214 and miR-331-3p in regulation of immune responses to Salmonella infection.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Hua
authors.initials: H
authors.lastname: Bao
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Arun
authors.initials: A
authors.lastname: Kommadath
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Guanxiang
authors.initials: G
authors.lastname: Liang
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Xu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Adriano S
authors.initials: AS
authors.lastname: Arantes
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, 50011 United States.
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Tuggle
authors.affiliation: Food Safety &Enteric Pathogens Research Unit, USDA/ARS/National Animal Disease Center, Ames, Iowa, 50010 USA.
authors.forename: Shawn M D
authors.initials: SM
authors.lastname: Bearson
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Graham S
authors.initials: GS
authors.lastname: Plastow
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Stothard
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G2P5 Canada.
authors.forename: Le Luo
authors.initials: le L
authors.lastname: Guan
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics; D005517:Foodborne Diseases / Q000382:microbiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D054884:Host-Pathogen Interactions / Q000235:genetics; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood; D015203:Reproducibility of Results; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000276:immunology; D034421:Sus scrofa / Q000235:genetics* / Q000382:microbiology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D000099173:Natural Resistance-associated Macrophage Protein 1