title: Effect of vascular bradykinin on pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis.
abstract: OBJECTIVE
  To investigate the effect of vascular bradykinin on pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis (SAP).
  
  
  METHODS
  Ninety male Wistar rats were randomly divided into a blank control group, an SAP group and a vascular bradykinin treatment group. The SAP model was induced by the retrograde injection of 5% sodium taurocholate in the pancreaticobiliary duct. The vascular bradykinin treatment group underwent gastrostomy, with a fine plastic tube placed in the stomach that led out of body through the abdominal wall.Vascular bradykinin was fully dissolved and administered at a dose of 20 U/kg once every 8 h. The pancreatic microcirculatory blood flow volume and velocity, microvascular permeability, hemorheology were evaluated respectively by double-channel laser Doppler flowmetry, the Evans blue leakage test, a blood rheology test instrument.
  
  
  RESULTS
  The pancreatic microcirculatory blood flow volume and velocity in the vascular bradykinin treatment group increased gradually after 48 h compared with the SAP group, and the changes were significantly different (p < 0.05). The pancreatic microvascular permeability of the vascular bradykinin treatment group was significantly reduced after 48 h compared with the SAP group (p < 0.05). The low shear rate blood viscosity, hematocrit and erythrocyte aggregation index of the vascular bradykinin treatment group were significantly decreased after 48 h compared with the SAP group (p < 0.05).
  
  
  CONCLUSIONS
  Vascular bradykinin can improve pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis.
authors.affiliation: Department of General Surgery, The No. 4 Affiliated Hospital of Hebei Medical University, Shijiazhuang, China. yonglicn@yeah.net.
authors.forename: L-T
authors.initials: LT
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: L-Q
authors.initials: LQ
authors.lastname: Fan
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: S-J
authors.initials: SJ
authors.lastname: Cheng
authors.forename: A-M
authors.initials: AM
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: B
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000008:administration & dosage*; D018056:Hemorheology / Q000187:drug effects* / Q000502:physiology; D007263:Infusions, Parenteral; D008297:Male; D008833:Microcirculation / Q000187:drug effects* / Q000502:physiology; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects*; D010195:Pancreatitis / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D016896:Treatment Outcome
chemical_list: D001920:Bradykinin