title: Viral gene expression potentiates reovirus-induced necrosis.
abstract: Infection of some cell types by reovirus evokes a caspase-independent form of cell death resembling necrosis. While reovirus strain T3D induces necrosis much more efficiently than strain T1L, which viral components contribute to this difference is not known. In this study, we identified that the sialic acid binding property of the reovirus σ1 protein affects necrosis efficiency. We found that in addition to sialic acid engagement by the virus particles, viral gene expression, in the form of viral RNA or protein synthesis, is also required for necrosis induction. Our studies reveal that sialic acid does not directly participate in necrosis induction by initiating a signaling pathway. Instead, sialic acid engagement augments necrosis induction indirectly, by increasing reovirus gene expression in each infected cell. Comparison of our results with previous studies suggests that reovirus-induced apoptosis and necrosis are initiated by distinct stages of viral infection.
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN 47405, United States.
authors.forename: Bradley E
authors.initials: BE
authors.lastname: Hiller
authors.affiliation: Interdisciplinary Program in Biochemistry, Indiana University, Bloomington, IN 47405, United States.
authors.forename: Angela K
authors.initials: AK
authors.lastname: Berger
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN 47405, United States; Interdisciplinary Program in Biochemistry, Indiana University, Bloomington, IN 47405, United States.. Electronic address: pdanthi@indiana.edu.
authors.forename: Pranav
authors.initials: P
authors.lastname: Danthi
mesh_terms: D016923:Cell Death; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D011485:Protein Binding; D012367:RNA, Viral / Q000096:biosynthesis; D012087:Reoviridae / Q000235:genetics*; D012794:Sialic Acids / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D012367:RNA, Viral; D012794:Sialic Acids; D014764:Viral Proteins
keywords: Cell death; Necrosis; Reovirus