title: Data for iTRAQ-based quantitative proteomics analysis of Brassica napus leaves in response to chlorophyll deficiency.
abstract: The essential pigment chlorophyll (Chl) plays important roles in light harvesting and energy transfer during photosynthesis. Here we present the data from a comparative proteomic analysis of chlorophyll-deficient Brassica napus mutant cde1 and its corresponding wild-type using the iTRAQ approach (Pu Chu et al., 2014 [1]). The distribution of length and number of peptides, mass and sequence coverage of proteins identified was calculated, and the repeatability of the replicates was analyzed. A total of 443 differentially expressed proteins were identified in B. napus leaves, including 228 down-accumulated proteins mainly involved in photosynthesis, porphyrin and chlorophyll metabolism, biosynthesis of secondary metabolites, carbon fixation and 215 up-accumulated proteins that enriched in the spliceosome, mRNA surveillance and RNA degradation.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Gui Xia
authors.initials: GX
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Li Na
authors.initials: LN
authors.lastname: Zhai
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Feng Qi
authors.initials: FQ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China ; Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
authors.forename: Rong Zhan
authors.initials: RZ
authors.lastname: Guan