title: Proteome-Wide Profiling of Targets of Cysteine reactive Small Molecules by Using Ethynyl Benziodoxolone Reagents.
abstract: In this study, we present a highly efficient method for proteomic profiling of cysteine residues in complex proteomes and in living cells. Our method is based on alkynylation of cysteines in complex proteomes using a "clickable" alkynyl benziodoxolone bearing an azide group. This reaction proceeds fast, under mild physiological conditions, and with a very high degree of chemoselectivity. The formed azide-capped alkynyl-cysteine adducts are readily detectable by LC-MS/MS, and can be further functionalized with TAMRA or biotin alkyne via CuAAC. We demonstrate the utility of alkynyl benziodoxolones for chemical proteomics applications by identifying the proteomic targets of curcumin, a diarylheptanoid natural product that was and still is part of multiple human clinical trials as anticancer agent. Our results demonstrate that curcumin covalently modifies several key players of cellular signaling and metabolism, most notably the enzyme casein kinase I gamma. We anticipate that this new method for cysteine profiling will find broad application in chemical proteomics and drug discovery.
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva, 30 quai Ernest-Ansermet, Geneva (Switzerland).
authors.forename: Daniel
authors.initials: D
authors.lastname: Abegg
authors.affiliation: Laboratory of Catalysis and Organic Synthesis, Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC LCSO, BCH 4306, 1015 Lausanne (Switzerland).
authors.forename: Reto
authors.initials: R
authors.lastname: Frei
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva, 30 quai Ernest-Ansermet, Geneva (Switzerland).
authors.forename: Luca
authors.initials: L
authors.lastname: Cerato
authors.affiliation: Laboratory of Catalysis and Organic Synthesis, Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC LCSO, BCH 4306, 1015 Lausanne (Switzerland).
authors.forename: Durga
authors.initials: D
authors.lastname: Prasad Hari
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva, 30 quai Ernest-Ansermet, Geneva (Switzerland).
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Laboratory of Catalysis and Organic Synthesis, Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC LCSO, BCH 4306, 1015 Lausanne (Switzerland).
authors.forename: Jerome
authors.initials: J
authors.lastname: Waser
authors.affiliation: School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva, 30 quai Ernest-Ansermet, Geneva (Switzerland). alexander.adibekian@unige.ch.
authors.forename: Alexander
authors.initials: A
authors.lastname: Adibekian
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D003545:Cysteine / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D010093:Oxolinic Acid / Q000737:chemistry*; D020543:Proteome; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D020543:Proteome; D003545:Cysteine; D010093:Oxolinic Acid
keywords: activity-based protein profiling; curcumin; mass spectrometry; proteomics; target identification