title: Biochemical Characterization of the Split Class II Ribonucleotide Reductase from Pseudomonas aeruginosa.
abstract: The opportunistic pathogen Pseudomonas aeruginosa can grow under both aerobic and anaerobic conditions. Its flexibility with respect to oxygen load is reflected by the fact that its genome encodes all three existing classes of ribonucleotides reductase (RNR): the oxygen-dependent class I RNR, the oxygen-indifferent class II RNR, and the oxygen-sensitive class III RNR. The P. aeruginosa class II RNR is expressed as two separate polypeptides (NrdJa and NrdJb), a unique example of a split RNR enzyme in a free-living organism. A split class II RNR is also found in a few closely related γ-Proteobacteria. We have characterized the P. aeruginosa class II RNR and show that both subunits are required for formation of a biologically functional enzyme that can sustain vitamin B12-dependent growth. Binding of the B12 coenzyme as well as substrate and allosteric effectors resides in the NrdJa subunit, whereas the NrdJb subunit mediates efficient reductive dithiol exchange during catalysis. A combination of activity assays and activity-independent methods like surface plasmon resonance and gas phase electrophoretic macromolecule analysis suggests that the enzymatically active form of the enzyme is a (NrdJa-NrdJb)2 homodimer of heterodimers, and a combination of hydrogen-deuterium exchange experiments and molecular modeling suggests a plausible region in NrdJa that interacts with NrdJb. Our detailed characterization of the split NrdJ from P. aeruginosa provides insight into the biochemical function of a unique enzyme known to have central roles in biofilm formation and anaerobic growth.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles väg 2, SE-17177, Stockholm, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Crona
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, SE-90187, Umeå, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Hofer
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles väg 2, SE-17177, Stockholm, Sweden.
authors.forename: Juan
authors.initials: J
authors.lastname: Astorga-Wells
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, SE-10691, Stockholm, Sweden.
authors.forename: Britt-Marie
authors.initials: BM
authors.lastname: Sjöberg
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles väg 2, SE-17177, Stockholm, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Tholander
mesh_terms: D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012264:Ribonucleotide Reductases / Q000378:metabolism*
chemical_list: D012264:Ribonucleotide Reductases