title: Membrane topology of Golgi-localized probable S-adenosylmethionine-dependent methyltransferase in tobacco (Nicotiana tabacum) BY-2 cells.
abstract: S-adenosylmethionine (SAM)-dependent methyltransferases (MTases) transfer methyl groups to substrates. In this study, a novel putative tobacco SAM-MTase termed Golgi-localized methyl transferase 1 (GLMT1) has been characterized. GLMT1 is comprised of 611 amino acids with short N-terminal region, putative transmembrane region, and C-terminal SAM-MTase domain. Expression of monomeric red fluorescence protein (mRFP)-tagged protein in tobacco BY-2 cell indicated that GLMT1 is a Golgi-localized protein. Analysis of the membrane topology by protease digestion suggested that both C-terminal catalytic region and N-terminal region seem to be located to the cytosolic side of the Golgi apparatus. Therefore, GLMT1 might have a different function than the previously studied SAM-MTases in plants.
authors.affiliation: a Laboratory of Plant Nutrition, Graduate School of Bioresource and Bioenvironmental Sciences , Kyushu University , Fukuoka , Japan.
authors.forename: Jianping
authors.initials: J
authors.lastname: Liu
authors.affiliation: a Laboratory of Plant Nutrition, Graduate School of Bioresource and Bioenvironmental Sciences , Kyushu University , Fukuoka , Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: a Laboratory of Plant Nutrition, Graduate School of Bioresource and Bioenvironmental Sciences , Kyushu University , Fukuoka , Japan.|b Laboratory of Plant Nutrition, Faculty of Agriculture , Kyushu University , Fukuoka , Japan.|c Biotron Application Center , Kyushu University , Fukuoka , Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Matsuoka
mesh_terms: D020126:Brefeldin A / Q000494:pharmacology; D002460:Cell Line; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D012436:S-Adenosylmethionine / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology*
chemical_list: D020126:Brefeldin A; D012436:S-Adenosylmethionine; D008780:Methyltransferases
keywords: Golgi apparatus; methytransferase; tobacco BY-2 cell