title: Metabolic engineering of Escherichia coli for production of biodiesel from fatty alcohols and acetyl-CoA.
abstract: Microbial production of biodiesel from renewable feedstock has attracted intensive attention. Biodiesel is known to be produced from short-chain alcohols and fatty acyl-CoAs through the expression of wax ester synthase/fatty acyl-CoA: diacylglycerol acyltransferase that catalyzes the esterification of short-chain alcohols and fatty acyl-CoAs. Here, we engineered Escherichia coli to produce various fatty alcohol acetate esters, which depend on the expression of Saccharomyces cerevisiae alcohol acetyltransferase ATF1 that catalyzes the esterification of fatty alcohols and acetyl-CoA. The fatty acid biosynthetic pathways generate fatty acyl-ACPs, fatty acyl-CoAs, or fatty acids, which can be converted to fatty alcohols by fatty acyl-CoA reductase, fatty acyl-ACP reductase, or carboxylic acid reductase, respectively. This study showed the biosynthesis of biodiesel from three fatty acid biosynthetic pathway intermediates.
authors.affiliation: College of Life and Environmental Sciences, Gannan Normal University, Ganzhou, People's Republic of China, ggdy3478@163.com.
authors.forename: Daoyi
authors.initials: D
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Pan
authors.forename: Xun
authors.initials: X
authors.lastname: Li
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D056804:Biofuels; D001711:Biotransformation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005233:Fatty Alcohols / Q000378:metabolism*; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D056804:Biofuels; D005233:Fatty Alcohols; D011506:Proteins; D011994:Recombinant Proteins; D000105:Acetyl Coenzyme A; C079238:alcohol O-acetyltransferase