title: High diversity of non-sporulating moulds in respiratory specimens of immunocompromised patients: should all the species be reported when diagnosing invasive aspergillosis?
abstract: Non-sporulating moulds (NSMs) isolated from respiratory specimens are usually discarded without further testing although they may have pathogenic effects in immunocompromised patients. The objective of this study was to determine the identity and frequency of NSMs in patients with haematological malignancies. We analysed the mycological results of 251 consecutive respiratory samples from 104 haematology patients. Yeast and sporulating moulds were identified at the genus/species level according to their phenotypic features. NSMs were identified by internal transcribed spacer (ITS) sequencing. We detected 179 positive samples, of which 10.1% (18/179) were mixtures of moulds and 26.3% (47/179) were mixtures of moulds and yeast. We identified 142 moulds belonging to 11 different genera/species or groups, with Aspergillus fumigatus (n = 50), Penicillium spp. (n = 31) and NSM (n = 24) being the most frequently isolated species. Twenty-two NSMs were successfully sequenced: 18 were basidiomycetes and six were ascomycetes, corresponding to 16 different genera/species. NSMs were isolated with A. fumigatus in the same sample or in a subsequent sample in five patients with probable invasive aspergillosis. The conclusion is that the respiratory specimens of immunocompromised patients frequently contain very diverse mould species that may increase the virulence of pathogenic species. Reporting all mould species isolated when diagnosing invasive fungal infection could test this hypothesis.
authors.affiliation: Institut Pasteur, Molecular Mycology Unit, National Reference Center of Invasive Mycoses and Antifungals, Paris, France.|CNRS URA3012, Paris, France.
authors.forename: Dea
authors.initials: D
authors.lastname: Garcia-Hermoso
authors.affiliation: Institut Pasteur, Molecular Mycology Unit, National Reference Center of Invasive Mycoses and Antifungals, Paris, France.|CNRS URA3012, Paris, France.|Laboratoire de Parasitologie-Mycologie, Groupe hospitalier Lariboisière-Saint Louis, Assistance Publique-Hôpitaux de Paris (APHP), Paris, France.|Sorbonne Paris Cité, Université Paris Diderot, Paris, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Alanio
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Groupe hospitalier Albert Chenevier-Henri Mondor, Assistance Publique-Hôpitaux de Paris (APHP), Créteil, France.|Université Paris-Est-Créteil, Créteil, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Cabaret
authors.affiliation: Laboratoire CERBA, Cergy Pontoise, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Olivi
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Groupe hospitalier Albert Chenevier-Henri Mondor, Assistance Publique-Hôpitaux de Paris (APHP), Créteil, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Foulet
authors.affiliation: Université Paris-Est-Créteil, Créteil, France.|Département d'Hématologie, Groupe Hospitalier Albert Chenevier-Henri Mondor, Assistance Publique-Hôpitaux de Paris (APHP), Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Cordonnier
authors.affiliation: Laboratoire CERBA, Cergy Pontoise, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Costa
authors.affiliation: Institut Pasteur, Molecular Mycology Unit, National Reference Center of Invasive Mycoses and Antifungals, Paris, France.|CNRS URA3012, Paris, France.|Laboratoire de Parasitologie-Mycologie, Groupe hospitalier Lariboisière-Saint Louis, Assistance Publique-Hôpitaux de Paris (APHP), Paris, France.|Sorbonne Paris Cité, Université Paris Diderot, Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bretagne
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D002648:Child; D002675:Child, Preschool; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D019337:Hematologic Neoplasms / Q000150:complications / Q000382:microbiology; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D055744:Invasive Pulmonary Aspergillosis / Q000175:diagnosis* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D010407:Penicillium / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000254:growth & development; D055815:Young Adult
chemical_list: D004271:DNA, Fungal
keywords: Respiratory specimens; immunocompromised patient; invasive aspergillosis; non-sporulating mould