title: MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2).
abstract: Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors.
  
  
  OBJECTIVE
  The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue.
  
  
  METHODS
  Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used.
  
  
  RESULTS
  Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive.
  
  
  CONCLUSIONS
  Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.
authors.affiliation: Facultad de Odontología, Universidad Nacional Autónoma de México, México, D.F, México.
authors.forename: Santa
authors.initials: S
authors.lastname: Ponce-Bravo
authors.affiliation: Facultad de Odontología, Universidad Nacional Autónoma de México, México, D.F, México.
authors.forename: Constantino
authors.initials: C
authors.lastname: Ledesma-Montes
authors.affiliation: Facultad de Odontología, Universidad Nacional Autónoma de México, México, D.F, México.
authors.forename: Maricela
authors.initials: M
authors.lastname: Garcés-Ortíz
mesh_terms: D008949:Adenoma, Pleomorphic / Q000737:chemistry* / Q000473:pathology; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D016022:Case-Control Studies; D006017:Glycolipids; D006023:Glycoproteins; D006801:Humans; D007150:Immunohistochemistry; D066292:Lipid Droplets; D008565:Membrane Proteins; D018396:Mucin-1 / Q000032:analysis*; D016612:Paraffin Embedding; D012016:Reference Values; D012468:Salivary Gland Neoplasms / Q000737:chemistry* / Q000473:pathology; D012469:Salivary Glands / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D006017:Glycolipids; D006023:Glycoproteins; C060192:MUC1 protein, human; D008565:Membrane Proteins; D018396:Mucin-1; C110367:milk fat globule