title: The histone acetyltransferase PsGcn5 mediates oxidative stress responses and is required for full virulence of Phytophthora sojae.
abstract: In eukaryotic organisms, histone acetyltransferase complexes are coactivators that are important for transcriptional activation by modifying chromatin. In this study, a gene (PsGcn5) from Phytophthora sojae encoding a histone acetyltransferase was identified as a homolog of one component of the histone acetyltransferase complex from yeasts to mammals. PsGcn5 was constitutively expressed in each stage tested, but had a slightly higher expression in sporulating hyphae and 3 h after infection. PsGcn5-silenced mutants were generated using polyethylene glycol-mediated protoplast stable transformation. These mutants had normal development, but compared to wild type strains they had higher sensitivity to hydrogen peroxide (H2O2) and significantly reduced virulence in soybean. Diaminobenzidine staining revealed an accumulation of H2O2 around the infection sites of PsGcn5-silenced mutants but not for wild type strains. Inhibition of the plant NADPH oxidase by diphenyleneiodonium prevented host-derived H2O2 accumulation in soybean cells and restored infectious hyphal growth of the mutants. Thus, we concluded that PsGcn5 is important for growth under conditions of oxidative stress and contributes to the full virulence of P. sojae by suppressing the host-derived reactive oxygen species.
authors.affiliation: Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China; Scientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, Hefei, Anhui, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China; Scientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, Hefei, Anhui, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China.
authors.forename: Shusen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China; Scientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, Hefei, Anhui, China. Electronic address: rende7@126.com.
authors.forename: Rende
authors.initials: R
authors.lastname: Qi
mesh_terms: D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis; D018384:Oxidative Stress; D010838:Phytophthora / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology; D013025:Glycine max; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D037521:Virulence Factors; D006861:Hydrogen Peroxide; D051548:Histone Acetyltransferases
keywords: Histone acetyltransferase; Oxidative stress; Phytophthora sojae; Virulence