title: Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures.
abstract: Printable scaffolds with adequate mechanical strength and stiffness are sought after to ensure viability of printed cells and tissues. We report the first peptide bioinks-lysine-containing hexapeptides that self-assemble into stable, nanofibrous three-dimensional hydrogels with unprecedented stiffness of up to 40 kPa. These biocompatible scaffolds support the three-dimensional culture of human stem cells and differentiation of primary cells into organotypic (gastrointestinal and skin) structures for high-throughput screening, diagnosis, and tissue engineering.
authors.affiliation: Institute of Bioengineering and Nanotechnology , 31 Biopolis Way, The Nanos, #04-01, Singapore 138669.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Loo
authors.affiliation: Institute of Bioengineering and Nanotechnology , 31 Biopolis Way, The Nanos, #04-01, Singapore 138669.
authors.forename: Anupama
authors.initials: A
authors.lastname: Lakshmanan
authors.affiliation: Institute of Bioengineering and Nanotechnology , 31 Biopolis Way, The Nanos, #04-01, Singapore 138669.
authors.forename: Ming
authors.initials: M
authors.lastname: Ni
authors.affiliation: Institute of Bioengineering and Nanotechnology , 31 Biopolis Way, The Nanos, #04-01, Singapore 138669.
authors.forename: Lai Ling
authors.initials: LL
authors.lastname: Toh
authors.affiliation: Department of Pathology, National University Hospital , 5 Lower Kent Ridge Road, 1 Main Building, Level 3, Singapore 119074.
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Bioengineering and Nanotechnology , 31 Biopolis Way, The Nanos, #04-01, Singapore 138669.
authors.forename: Charlotte A E
authors.initials: CA
authors.lastname: Hauser
mesh_terms: D018938:Caco-2 Cells; D006801:Humans; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D010455:Peptides / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D010455:Peptides
keywords: Peptide bioink; bioprinting; nanofibrous scaffold; smart biomaterials; stimuli-responsive self-assembly; ultrashort peptides