title: Glucagon-like peptide-2-loaded microspheres as treatment for ulcerative colitis in the murine model.
abstract: Glucagon-like peptide-2 (GLP-2) is an intestinal hormone that promotes intestinal growth, but the rapid degradation by dipeptidyl peptidase-IV limits its applications. PLGA microsphere is a well-developed drug delivery system, while seldom been studied as a solution for prolonging in vivo effects of GLP-2. In this study, we encapsulated porcine GLP-2 (pGLP-2) into microspheres and investigated its therapeutic effects in dextran sulfate sodium (DSS)-treated mice. pGLP-2 microspheres showed 20.36% in initial burst and constant release for at least 9 d. In the DSS-treated mice, a single injection of GLP-2 microspheres significantly increased the body weight, colonic length, small intestinal weight and mRNA expression of Occludin, decreased the colonic damage score, mRNA expression of IL-6, IL-10, TNF-α and IFN-γ. In conclusion, pGLP-2 microspheres were resistant to degradation and decreased the severity of DSS-induced ulcerative colitis which suggested that GLP-2-loaded microspheres could be a proper candidate for the treatment of ulcerative colitis.
authors.affiliation: a Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences , Hangzhou , People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Keke
authors.initials: K
authors.lastname: Qi
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Wan
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology*; D003106:Colon / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D053767:Glucagon-Like Peptide 2 / Q000737:chemistry*; D007107:Immune System; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D062793:Occludin / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053767:Glucagon-Like Peptide 2; D007375:Interleukin-1; D015850:Interleukin-6; D062793:Occludin; C568986:Ocln protein, mouse; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016264:Dextran Sulfate
keywords: DSS; PLGA; microspheres; pGLP-2; ulcerative colitis