title: The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis.
abstract: The metazoan nucleus breaks down and reassembles during each cell division. Upon mitotic exit, the successful reestablishment of an interphase nucleus requires the coordinated reorganization of chromatin and formation of a functional nuclear envelope. Here, we report that the histone demethylase LSD1 (also known as KDM1A) plays a crucial role in nuclear assembly at the end of mitosis. Downregulation of LSD1 in cells extends telophase and impairs nuclear pore complex assembly. In vitro, LSD1 demethylase activity is required for the recruitment of MEL28 (also known as ELYS and AHCTF1) and nuclear envelope precursor vesicles to chromatin, crucial steps in nuclear reassembly. Accordingly, the formation of a closed nuclear envelope and nuclear pore complex assembly are impaired upon depletion of LSD1 or inhibition of its activity. Our results identify histone demethylation by LSD1 as a new regulatory mechanism linking the chromatin state and nuclear envelope formation at the end of mitosis.
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany.
authors.forename: Allana
authors.initials: A
authors.lastname: Schooley
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Moreno-Andrés
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany.
authors.forename: Paola
authors.initials: P
authors.lastname: De Magistris
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Vollmer
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany wolfram.antonin@tuebingen.mpg.de.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Antonin
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly; D006367:HeLa Cells; D056466:Histone Demethylases / Q000378:metabolism*; D006801:Humans; D009685:Nuclear Envelope / Q000378:metabolism*; D013692:Telophase / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D056466:Histone Demethylases
keywords: ELYS; Histone modification; KDM1A; Lysine (K)-specific demethylase 1A; MEL28; Mitotic exit; NDC1; Nuclear envelope formation; Nuclear pore complex; POM121