title: Ultramicro chitosan-assisted in-syringe dispersive micro-solid-phase extraction for flavonols from healthcare tea by ultra-high performance liquid chromatography.
abstract: A novel dispersive micro-solid-phase extraction (DMSPE) method using ultramicro chitosan as an adsorbent was proposed for the analysis of flavonols in Flos Sophorae tea, with detection by ultra-high performance liquid chromatography. Various parameters affecting the extraction recovery, including the concentration and viscosity of chitosan, extraction time and the pH of the sample solution were investigated and optimized. The optimized conditions were found to be 0.4μg/mL of sorbent, pH of 7, an extraction time of 1.0min and 100μL of methanol as the elution solvent. The DMSPE method showed excellent linearity (r(2)>0.99) with pre-concentration factor values ranging from 23 to 77-fold. The limits of detection and quantification were 0.22-0.36ng/mL and 0.72-1.21ng/mL, respectively. This method was successfully applied to the analysis of flavonols in Flos Sophorae tea samples, and their recoveries were in the range of 95.2-102.4%.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: Hangzhou Red Cross Hospital, Department of Traditional Chinese Medicine, Hangzhou 310003, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: Zhang
mesh_terms: D001628:Beverages / Q000032:analysis*; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D029909:Sophora / Q000737:chemistry*; D013594:Syringes
chemical_list: D044948:Flavonols; D048271:Chitosan
keywords: Chitosan; Dispersive micro-solid-phase extraction; Flavonols; Flos Sophorae; Syringe; Ultra-high performance liquid chromatography