title: Effects of cilengitide in osteoclast maturation and behavior.
abstract: Bone metastasis is a common burden in many types of cancer and has a severe impact on the quality of life in patients. Hence, specific therapeutic strategies inhibiting tumor induced osteolysis are urgently needed. In this study, we aimed to interfere with integrin adhesion receptors, which are central players of the bone resorption process. For this purpose, we used cilengitide, a cyclic RGD peptide, which blocks integrin αVβ3 and αVβ5-ligand binding. Our results revealed that cilengitide blocked osteoclast maturation in a dose-dependent manner. In detail, pre-osteoclasts treated with cilengitide exhibited reduced cell spreading, cell migration and cell adhesion on RGD-containing matrix proteins, which are ligands of integrin αV. The activation of the most upstream signal transduction molecules of the integrin receptor-initiated pathway, FAK and c-Src, were consistently blocked by cilengitide. First evidence suggests that cilengitide might interfere with metastatic bone disease in vivo and this study describes a potential underlying mechanism of the inhibitory effect of cilengitide on αV-integrin expressing pre-osteoclasts by blocking integrin ligand binding and interfering with osteoclast maturation and cell behavior. In conclusion, our findings suggest that cilengitide, which interferes with αV-integrins on osteoclasts, may represent a novel therapeutic strategy in the treatment of malignant bone disease.
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Chillà
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Daniela
authors.initials: D
authors.lastname: Bianconi
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Nisha
authors.initials: N
authors.lastname: Geetha
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Dorda
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Marina
authors.initials: M
authors.lastname: Poettler
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Unseld
authors.affiliation: Division of Rheumatology, Department of Medicine III, Medical University of Vienna, Vienna, Austria.
authors.forename: Despoina
authors.initials: D
authors.lastname: Sykoutri
authors.affiliation: Division of Rheumatology, Department of Medicine III, Medical University of Vienna, Vienna, Austria.
authors.forename: Kurt
authors.initials: K
authors.lastname: Redlich
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria.
authors.forename: Christoph C
authors.initials: CC
authors.lastname: Zielinski
authors.affiliation: Department of Medicine I, Comprehensive Cancer Center Vienna, Medical University of Vienna, Austria. Electronic address: gerald.prager@meduniwien.ac.at.
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Prager
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000517:prevention & control* / Q000556:secondary; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D039001:Integrin alpha Chains / Q000037:antagonists & inhibitors / Q000378:metabolism; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects; D012910:Snake Venoms / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D039001:Integrin alpha Chains; D012910:Snake Venoms; C422910:Cilengitide
keywords: Integrin; Metastatic bone disease; Osteoclast; RGD-peptide