title: A transgenic quail model that enables dynamic imaging of amniote embryogenesis.
abstract: Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.
authors.affiliation: Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
authors.forename: David
authors.initials: D
authors.lastname: Huss
authors.affiliation: Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS (UMR 7104), Illkirch 67400, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Benazeraf
authors.affiliation: Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
authors.forename: Allison
authors.initials: A
authors.lastname: Wallingford
authors.affiliation: Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Filla
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 94720, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA Division of Biology, California Institute of Technology, Pasadena, CA 94720, USA.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Fraser
authors.affiliation: Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA Division of Biology, California Institute of Technology, Pasadena, CA 94720, USA Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA lansford@usc.edu.
authors.forename: Rusty
authors.initials: R
authors.lastname: Lansford
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D049109:Cell Proliferation / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D016086:Lentivirus; D008164:Luminescent Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D023421:Models, Animal; D009024:Morphogenesis / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D011784:Quail; D059008:Time-Lapse Imaging / Q000379:methods*
chemical_list: D008164:Luminescent Proteins
keywords: Confocal microscopy; Live imaging; Quail embryo