title: A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases.
abstract: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
authors.affiliation: Instituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
authors.forename: Leticia Mara Lima
authors.initials: LM
authors.lastname: Angelini
authors.affiliation: Instituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
authors.forename: Amanda Ribeiro Martins
authors.initials: AR
authors.lastname: da Silva
authors.affiliation: Instituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
authors.forename: Lucas de Freitas Coli
authors.initials: Lde F
authors.lastname: Rocco
authors.affiliation: Departamento de Química Orgânia, Instituto de Química, Universidade Estadual Paulista "Julio de Mesquita Filho", Araraquara, SP, Brazil.
authors.forename: Cintia Duarte de Freitas
authors.initials: CD
authors.lastname: Milagre
mesh_terms: D000581:Amidohydrolases / Q000037:antagonists & inhibitors; D000619:Aminohydrolases / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006836:Hydro-Lyases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration
chemical_list: D000581:Amidohydrolases; C019665:amidase; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C048831:nitrile hydratase
keywords: amidase; amidase inhibitor; high-throughput screening; nitrilase; nitrile hydratase