title: Biocompatible chiral monolithic stationary phase synthesized via atom transfer radical polymerization for high performance liquid chromatographic analysis.
abstract: Novel biocompatible chiral monolithic stationary phase was prepared by reverse and direct atom transfer radical polymerization (ATRP) methods. By taking advantages of the controlled/living property of ATRP method, the chiral monolith was prepared by reverse ATRP (RATRP) firstly. An attractive feature of RATRP is the prepared polymer containing a terminal radically transferable atom that can initiate another post-polymerization reaction by direct ATRP. Then, the biocompatible poly(hydroxyethyl methacrylate) (PHEMA) was grafted on the surface of the chiral monolith by direct ATRP as a diffusion barrier for proteins. This biocompatible chiral monolith was successfully used as restricted access stationary phase for determination of enantiomers in biological samples with direct injection by high-performance liquid chromatography (HPLC).
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, China. Electronic address: wanghuai1234@gmail.com.
authors.forename: Huai-Song
authors.initials: HS
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xia-Yi
authors.initials: XY
authors.lastname: Feng
authors.affiliation: Tianjin Modern Vocational Technology College, Tianjin 300350, China.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Wei
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D058105:Polymerization; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; D011102:Polyhydroxyethyl Methacrylate; C031215:betadex
keywords: Biological samples; Chiral monolithic stationary phase; Enantio-separation; Restricted access material; Reverse atom transfer radical polymerization; β-CD