title: Functional analysis of differentially expressed proteins in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits during ripening.
abstract: This study developed a proteome reference map of Myrica rubra fruits at the green, pink and red stages during ripening using two-dimensional gel electrophoresis (2-DE). Forty-six differentially expressed proteins were detected in the gel, of which 43 were successfully identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry and protein database searching. We found that malic enzyme related to the decrease of organic acid acidity was up-regulated. The high abundance of pyruvate decarboxylase and alcohol dehydrogenase may contribute to fruit peculiar fragrant characteristics. Phenylalanine ammonia-lyase, chalcone synthase 11, UDP-glucose:flavonoid 3-O-glucosyltransferase, and anthocyanidin synthase, enzymes involved in the anthocyanin metabolic pathway, were all up-regulated. The physiological data agree with fruit proteome results. These findings provided insights into the metabolic processes and regulatory mechanisms during Chinese bayberry fruit ripening.
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Yi-Yong
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Fruit Research Institute of Fujian Academy of Agriculture Science, Fuzhou 350013, China.
authors.forename: Ze-Huang
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Can-Yu
authors.initials: CY
authors.lastname: Zhong
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Song
authors.affiliation: Fruit Research Institute of Fujian Academy of Agriculture Science, Fuzhou 350013, China.
authors.forename: Qi-Hua
authors.initials: QH
authors.lastname: Lin
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Rong-Hui
authors.initials: RH
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: weichen@fafu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D031641:Myrica / Q000737:chemistry*
chemical_list: D000872:Anthocyanins
keywords: Chinese bayberry; Differential expression proteins; Fruit ripening; MALDI-TOF/TOF-MS; Two-dimensional gel electrophoresis