title: Emerging therapeutic delivery capabilities and challenges utilizing enzyme/protein packaged bacterial vesicles.
abstract: Nanoparticle-based therapeutics are poised to play a critical role in treating disease. These complex multifunctional drug delivery vehicles provide for the passive and active targeted delivery of numerous small molecule, peptide and protein-derived pharmaceuticals. This article will first discuss some of the current state of the art nanoparticle classes (dendrimers, lipid-based, polymeric and inorganic), highlighting benefits/drawbacks associated with their implementation. We will then discuss an emerging class of nanoparticle therapeutics, bacterial outer membrane vesicles, that can provide many of the nanoparticle benefits while simplifying assembly. Through molecular biology techniques; outer membrane vesicle hijacking potentially allows for stringent control over nanoparticle production allowing for targeted protein packaged nanoparticles to be fully synthesized by bacteria.
authors.affiliation: Postdoctoral Fellow - National Research Council.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Alves
authors.affiliation: Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375, USA.
authors.forename: Kendrick B
authors.initials: KB
authors.lastname: Turner
authors.affiliation: Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375, USA.
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
authors.affiliation: Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Walper
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D002462:Cell Membrane / Q000201:enzymology*; D004337:Drug Carriers; D004798:Enzymes / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D050997:Nanomedicine; D053758:Nanoparticles; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D013678:Technology, Pharmaceutical / Q000379:methods; D022161:Transport Vesicles / Q000201:enzymology*
chemical_list: D004337:Drug Carriers; D004798:Enzymes; D011994:Recombinant Proteins