title: Structural effects and nanoparticle size are essential for quantum dots-metallothionein complex formation.
abstract: Interaction between semiconductor nanocrystals, cadmium telluride quantum dots (CdTe QDs) capped with mercaptosuccinic acid (MSA) and metallothionein (MT) was investigated. MSA-capped CdTe QDs were synthesized in aqueous solution. Mixture of MT and MSA-capped CdTe QDs has been investigated by various analytical methods as follows: tris-tricine gel electrophoresis, fluorescence evaluation and electrochemical detection of catalysed hydrogen evolution. The obtained results demonstrate that MSA-capped CdTe QDs and MT do not create firmly bound stabile complex. However, weak electrostatic interactions contribute to the interaction of MT with MSA-capped CdTeQDs. It can be concluded that QDs size influences the QDs and MT interaction. The smallest QDs had the highest affinity to MT and vice versa.
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tmejova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Hynek
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic; Department of Pathological Physiology, Faculty of Medicine, Masaryk University, European Union, Brno, Czech Republic.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Gumulec
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Sona
authors.initials: S
authors.lastname: Krizkova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Guran
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Heger
authors.affiliation: Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, European Union, CZ-612 00 Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Kalina
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Vaculovicova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, European Union, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, European Union, CZ-616 00 Brno, Czech Republic. Electronic address: kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000818:Animals; D055664:Electrochemical Techniques; D008668:Metallothionein / Q000737:chemistry*; D015394:Molecular Structure; D053758:Nanoparticles; D045663:Quantum Dots; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008668:Metallothionein
keywords: Complex; Interaction; Metallothionein; Quantum dot