title: ATF5 Connects the Pericentriolar Materials to the Proximal End of the Mother Centriole.
abstract: Although it is known that the centrioles play instructive roles in pericentriolar material (PCM) assembly and that the PCM is essential for proper centriole formation, the mechanism that governs centriole-PCM interaction is poorly understood. Here, we show that ATF5 forms a characteristic 9-fold symmetrical ring structure in the inner layer of the PCM outfitting the proximal end of the mother centriole. ATF5 controls the centriole-PCM interaction in a cell-cycle- and centriole-age-dependent manner. Interaction of ATF5 with polyglutamylated tubulin (PGT) on the mother centriole and with PCNT in the PCM renders ATF5 as a required molecule in mother centriole-directed PCM accumulation and in PCM-dependent centriole formation. ATF5 depletion blocks PCM accumulation at the centrosome and causes fragmentation of centrioles, leading to the formation of multi-polar mitotic spindles and genomic instability. These data show that ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the PCM.
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
authors.forename: Bhanupriya
authors.initials: B
authors.lastname: Madarampalli
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Lengel
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
authors.forename: Yidi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA.
authors.forename: Jinming
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Xinyuan Institute of Medicine and Biotechnology, College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Brain Tumor Center and Department of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA. Electronic address: d.liu@wsu.edu.
authors.forename: David X
authors.initials: DX
authors.lastname: Liu
mesh_terms: D051696:Activating Transcription Factors / Q000378:metabolism*; D002502:Centrioles / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D015536:Down-Regulation; D042822:Genomic Instability; D006367:HeLa Cells; D006801:Humans; D008941:Spindle Apparatus / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: C507532:ATF5 protein, human; D051696:Activating Transcription Factors; D014404:Tubulin
keywords: ATF5; PCM; PCNT; centriole; centrosome; genomic instability; mitotic spindle; polyglutamylated tubulin