title: Spectroscopic investigations on the interactions of AgTiO2 nanoparticles with lysozyme and its influence on the binding of lysozyme with drug molecule.
abstract: Binding of lysozyme with AgTiO2 nanoparticles was analyzed by using absorption, fluorescence, time resolved and synchronous fluorescence measurements. In the presence of AgTiO2 nanoparticles, the fluorescence intensity of lysozyme was decreased. Static type of binding was confirmed through lifetime and ground state absorption measurements. From the fluorescence quenching data, the binding constant and the number of binding sites were found to be 1.5×10(4)M(-1) and 1.03, respectively. From the synchronous fluorescence spectroscopic measurements, tryptophan residue in lysozyme was found to have interaction with the nanoparticles. Further, the influence of AgTiO2 nanoparticles on the binding strength of lysozyme with a drug molecule was analyzed through fluorescence quenching methods. The presence of nanoparticles decreases the binding capability of drug with protein. Overall, the observed results will provide basic insights on the utilization of nanoparticles in drug delivery applications.
authors.affiliation: Department of Chemistry, Sri Ramakrishna Institute of Technology, Coimbatore 641010, Tamil Nadu, India. Electronic address: revathigr09@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Revathi
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Rameshkumar
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Sivasudha
mesh_terms: D001665:Binding Sites; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D004923:Erythrosine / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D018030:Silver Compounds / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D018030:Silver Compounds; C009495:titanium dioxide; D014025:Titanium; D009113:Muramidase; D004923:Erythrosine
keywords: AgTiO(2); Fluorescence quenching; Lysozyme; Synchronous fluorescence; Tryptophan