title: Antibiotic resistance and integrons in Shiga toxin-producing Escherichia coli (STEC).
abstract: Shiga toxin-producing Escherichia coli (STEC) cause hemorrhagic colitis (HC) and hemolytic-uremic syndrome in humans (HUS). Cattle are the main reservoir of STEC and transmission to humans occurs through contaminated food and water. Antibiotics are used in pig production systems to combat disease and improve productivity and play a key role in the dissemination of antibiotic resistance genes to the bacteria. Integrons have been identified in resistant bacteria allowing for the acquisition and dissemination of antibiotic resistance genes. STEC strains isolated from humans and animals have developed antibiotic resistance. In our laboratory, 21 non-157 STEC strains isolated from pigs were analyzed to detect class 1 and 2 integrons by PCR. Eight carried integrons, 7 of them harbored intl2. In another study 545 STEC strains were also analyzed for the presence of intl1 and intl2 . Strains carrying intl1 belonged to isolates from environment (n = 1), chicken hamburger (n = 2), dairy calves (n = 4) and pigs (n = 8). Two strains isolated from pigs harbored intl2 and only one intl1 / intl2 , highlighting the presence of intl2 in pigs. The selection for multiresistant strains may contribute to the emergence of antibiotic resistant pathogens and facilitate the spreading of the mobile resistance elements to other bacteria.
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil, Consejo Nacional the Investigaciones Científicas y Técnicas, Universidad Nacional del Centro de la Provincia de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Rocío
authors.initials: R
authors.lastname: Colello
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil, Consejo Nacional the Investigaciones Científicas y Técnicas, Universidad Nacional del Centro de la Provincia de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Analía I
authors.initials: AI
authors.lastname: Etcheverría
authors.affiliation: Laboratorio de Resistencia Microbiana, Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Di Conza
authors.affiliation: Laboratorio de Resistencia Microbiana, Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Gabriel O
authors.initials: GO
authors.lastname: Gutkind
authors.affiliation: Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil, Consejo Nacional the Investigaciones Científicas y Técnicas, Universidad Nacional del Centro de la Provincia de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Nora L
authors.initials: NL
authors.lastname: Padola
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002645:Chickens; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D024881:Drug Resistance, Bacterial; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D006801:Humans; D032023:Integrons; D008460:Meat / Q000382:microbiology; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents
keywords: STEC; antibiotics; integrons; multiresistance; public health