title: GmSBH1, a homeobox transcription factor gene, relates to growth and development and involves in response to high temperature and humidity stress in soybean.
abstract: GmSBH1 involves in response to high temperature and humidity stress. Homeobox transcription factors are key switches that control plant development processes. Glycine max H1 Sbh1 (GmSBH1) was the first homeobox gene isolated from soybean. In the present study, the full ORF of GmSBH1 was isolated, and the encoded protein was found to be a typical class I KNOX homeobox transcription factor. Subcellular localization and transcriptional activation assays showed that GmSBH1 is a nuclear protein and possesses transcriptional activation activity in the homeodomain. The KNOX1 domain was found to play a clear role in suppressing the transcriptional activation activity of GmSBH1. GmSBH1 showed different expression levels among different soybean tissues and was involved in response to high temperature and humidity (HTH) stress in developing soybean seeds. The overexpression of GmSBH1 in Arabidopsis altered leaf and stoma phenotypes and enhanced seed tolerance to HTH stress. Overall, our results indicated that GmSBH1 is involved in growth, development, and enhances tolerance to pre-harvest seed deterioration caused by HTH stress in soybean.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.|College of Agriculture, Anhui Science and Technology University, Fengyang, 233100, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Shu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Zhankui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Animal and Plant Introduction and Research Center, Shanghai Agricultural Academy, Shanghai, 201106, People's Republic of China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. Lq-ncsi@njau.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Ma
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006358:Hot Temperature; D006813:Humidity; D030262:Soybean Proteins / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C086263:H1 protein, Glycine max; D030262:Soybean Proteins; D014157:Transcription Factors
keywords: Growth and development; High temperature and humidity stress; Homeobox gene; Soybean; Transcriptional activation activity