title: Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization.
abstract: In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carrageenan (P(AAm-CA)/K-car) semi-interpenetrating network by entrapment method. Optimum pH and temperatures values were determined between 5.0-6.0 and 40-50 °C for free laccase (FL) and immobilized laccases, respectively. After 42 days of storage at 4 °C, FL and immobilized laccases retained their original activities in the range of 55%-73%. Percent decolorization of Acid Orange 52 by free enyzme and enyzmes immobilized in hydrogels was found between 63% and 39%. Percent decolorization of Acid Orange 52 in the presence of mediator by free enyzme was found 73% and enyzmes immobilized in hydrogels were found as 73%.
authors.affiliation: Süleyman Demirel University, Atabey Vocational High School, Isparta, Turkey.
authors.forename: Selva Zeynep
authors.initials: SZ
authors.lastname: Koklukaya
authors.affiliation: TUBITAK Marmara Research Center, Institute of Chemical Technology, Kocaeli, Turkey. serdar.sezer@tubitak.gov.tr.
authors.forename: Serdar
authors.initials: S
authors.lastname: Sezer
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, Teknikokullar, Ankara, Turkey. seraksoy@gazi.edu.tr.
authors.forename: Serpil
authors.initials: S
authors.lastname: Aksoy
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Middle East Technical University, Ankara, Turkey.|BIOMATEN Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, Ankara, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Hasirci
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001391:Azo Compounds / Q000737:chemistry* / Q000302:isolation & purification; D003116:Color; D004396:Coloring Agents / Q000737:chemistry* / Q000302:isolation & purification; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D055454:Trametes / Q000201:enzymology; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000302:isolation & purification; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001391:Azo Compounds; D004396:Coloring Agents; D004800:Enzymes, Immobilized; D020100:Hydrogels; D042845:Laccase; D014874:Water Pollutants, Chemical; D000099166:Polyacrylamides
keywords: Acid Orange 52; decolorization; immobilized enzyme; laccase; semi-IPNs