title: Role of Laryngopharyngeal Reflux in the Pathogenesis of Otitis Media with Effusion.
abstract: OBJECTIVE
  To determine whether there is an association between otitis media with effusion and laryngopharyngeal reflux in children.
  
  
  METHODS
  This study included 31 children with otitis media with effusion. The pepsinogen level in the middle ear fluid of all patients was measured by sandwich enzyme-linked immunosorbent assay. Each patient's middle ear fluid was investigated for Helicobacter pylori (H. pylori) using the Campylobacter-like organism (CLO) test. The middle ear pepsinogen levels were compared with those in the serum. The correlation between pepsinogen levels and H. pylori positivity in the middle ear fluid was investigated.
  
  
  RESULTS
  The mean middle ear pepsinogen level (211.69 ng/mL) was significantly higher than that in the serum (24.18 ng/mL) in patients with otitis media with effusion. The middle ear aspirates of six patients (19%) were positive for H. pylori, and the correlation between H. pylori positivity and increased pepsinogen levels in the middle ear fluid was statistically significant in patients with otitis media with effusion.
  
  
  CONCLUSIONS
  We detected higher pepsinogen levels and H. pylori positivity rates in the middle ear fluid than in the serum of patients with otitis media with effusion. These results support the role of laryngopharyngeal reflux in the pathogenesis of otitis media with effusion.
authors.affiliation: Department of Otorhinolaryngology, Adana Numune Training and Research Hospital, Adana, Turkey. drsuheylhayt@hotmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Doğru
authors.forename: Gökhan
authors.initials: G
authors.lastname: Kuran
authors.forename: Süheyl
authors.initials: S
authors.lastname: Haytoğlu
authors.forename: Ramazan
authors.initials: R
authors.lastname: Dengiz
authors.forename: Osman Kürşat
authors.initials: OK
authors.lastname: Arıkan
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005122:Exudates and Transudates / Q000737:chemistry; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D057045:Laryngopharyngeal Reflux / Q000150:complications* / Q000378:metabolism; D008297:Male; D010034:Otitis Media with Effusion / Q000209:etiology* / Q000378:metabolism; D020116:Pepsinogen A / Q000378:metabolism; D012189:Retrospective Studies
chemical_list: D020116:Pepsinogen A