title: Isolation and structural elucidation of a novel homogenous polysaccharide from Tricholoma matsutake.
abstract: A crude polysaccharide possessing antitumour, radiation-resistant and anti-ageing attributes was extracted from Tricholoma matsutake by water extraction and alcohol precipitation. From this crude polysaccharide, a homogeneous polysaccharide, TMP-5II, was successfully purified by Sephacryl S-300 column chromatography. The average molecular weight (Mw) of TMP-5II was 15.76 kDa. Monosaccharide analysis indicated that the homogeneous polysaccharide contained four different residues: d-glucose, d-galactose, d-mannose and d-fucose. Attenuated total reflectance infrared spectroscopy revealed characteristics typical of carbohydrate polymers and a peak typical of a β-type glycosidic bond. TMP-5II was selected for structural characterisation by nuclear magnetic resonance (NMR) analysis. According to (1)H NMR, (13)C NMR and two-dimensional-NMR analysis, TMP-5II contains two kinds of linkages, β and α, at a ratio of 4:1. Preliminary results indicated that the polysaccharide had (1-4)-beta-pyran glucose as the main chain, and a branched chain in the O-6 location with fucose (1-2) mannose (1-3)-alpha-pyran galactose.
authors.affiliation: a Beijing Key Laboratory of Plants Resource Research and Development, School of Science, Beijing Technology and Business University , Beijing 100048 , P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
authors.affiliation: a Beijing Key Laboratory of Plants Resource Research and Development, School of Science, Beijing Technology and Business University , Beijing 100048 , P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jia
authors.affiliation: a Beijing Key Laboratory of Plants Resource Research and Development, School of Science, Beijing Technology and Business University , Beijing 100048 , P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: b Infinitus International (China) Company, Ltd. , Guangzhou , Guangdong 510665 , P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liu
authors.affiliation: b Infinitus International (China) Company, Ltd. , Guangzhou , Guangdong 510665 , P.R. China.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Liu
authors.affiliation: a Beijing Key Laboratory of Plants Resource Research and Development, School of Science, Beijing Technology and Business University , Beijing 100048 , P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: a Beijing Key Laboratory of Plants Resource Research and Development, School of Science, Beijing Technology and Business University , Beijing 100048 , P.R. China.
authors.forename: Congfen
authors.initials: C
authors.lastname: He
mesh_terms: D002236:Carbohydrate Conformation; D011232:Chemical Precipitation; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides; D005947:Glucose; D005690:Galactose
keywords: 2D-NMR; GC-MS; Tricholoma matsutake; polysaccharide; structure elucidation