title: Evaluation of biological pretreatments to increase the efficiency of the saccharification process using Spartina argentinensis as a biomass resource.
abstract: Second generation bioethanol obtained from native perennial grasses offers a promising alternative for biofuel production, avoiding land use competition for crops production. Spartina argentinensis is a native perennial C4 grass with high photosynthetic rates, well adapted to halo-hydromorphic soils, though its forage quality (palatability and digestibility) for livestock is quite low due to its high lignin content. Hence, cattle raisers burn these grasslands frequently in order to stimulate the emergence of new leaves with higher digestibility for cattle feeding. Lignin is the main barrier to overcome in order to efficiently hydrolyze the cellulose for bioethanol production. In this work, we evaluate different pretreatments (phosphoric acid, ligninolytic enzymes and fungal supernatants) aimed to remove lignin and improving cellulose hydrolysis efficiency. Results show that pretreatment with Pycnoporus sanguineus supernatant improves fermentable carbohydrates availability, compared with a conventional chemical pretreatment, and that 56.84% of cellulose can be hydrolyzed using this pretreatment.
authors.affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Larran
authors.affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Jozami
authors.affiliation: Facultad de Ciencias Bioquimicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Santa Fe, Argentina.
authors.forename: Lionel
authors.initials: L
authors.lastname: Vicario
authors.affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina; Consejo de Investigaciones Universidad Nacional de Rosario, Maipú 1165, 2000 Rosario, Santa Fe, Argentina.
authors.forename: Susana R
authors.initials: SR
authors.lastname: Feldman
authors.affiliation: Facultad de Ciencias Bioquimicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Santa Fe, Argentina; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Suipacha 570, 2000 Rosario, Santa Fe, Argentina.
authors.forename: Florencio E
authors.initials: FE
authors.lastname: Podestá
authors.affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina. Electronic address: hperming@unr.edu.ar.
authors.forename: Hugo R
authors.initials: HR
authors.lastname: Permingeat
mesh_terms: D000818:Animals; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002417:Cattle; D002482:Cellulose / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry; D005285:Fermentation; D005658:Fungi; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D010756:Phosphoric Acids; D006109:Poaceae / Q000737:chemistry*
chemical_list: D056804:Biofuels; D004798:Enzymes; D010756:Phosphoric Acids; D002482:Cellulose; D008031:Lignin; C030242:phosphoric acid
keywords: Biomass; Lignocellulose; Pretreatments; Second generation bioethanol; White rot fungi