title: Assessment of the antidiabetic and antilipidemic properties of Bacillus subtilis SPB1 biosurfactant in alloxan-induced diabetic rats.
abstract: The present study aimed to scrutinize the potential of Bacillus subtilis SPB1biosurfactant, orally administered, for preventing diabetic complications in rats. The findings revealed that, Bacillus subtilis biosurfactant was an effective reducer of α-amylase activity in the plasma. Moreover, this supplement helped protect the β-cells from death and damage. Both the inhibitory action of SPB1 biosurfactant on α-amylase and the protection of the pancreas' β-cells lead to a decrease of the blood glucose levels, consequently antihyperglycemic effect. Interestingly, this lipopeptide biosurfactant modulated key enzyme related to hyperlipidemia as lipase; which leads to the regulation of the lipid profile in serum by the delay in the absorption of LDL-cholesterol and triglycerides, and a significant increase in HDL-cholesterol. Histological analyses also showed that it exerted a protective action on the pancreases and efficiently preserved the liver-kidney functions of diabetic rats, evidenced by significant decreases in aspartate transaminase, alanine transaminase, gamma-glytamyl transpeptidase and lactate deshydrogenase activities in the plasma, as well as in the creatinine and urea contents. Overall, the present study demonstrated that the hypoglycemic and antilipidemic activities exhibited by Bacillus subtilis biosurfactant were effective enough to alleviate induced diabetes in experimental rats. Therefore, SPB1biosurfactant could be considered as a potential strong candidate for the treatment and prevention of diabetes.
authors.affiliation: Unit "Enzymes and Bioconversion", National School of Engineers of Sfax (ENIS), Sfax, Tunisia.|Higher Institute of Biotechnology of Sfax (ISBS), Tunisia.
authors.forename: Raida
authors.initials: R
authors.lastname: Zouari
authors.affiliation: Animal Ecophysiology Laboratory, Faculty of Sciences, Sfax, Tunisia.
authors.forename: Rihab
authors.initials: R
authors.lastname: Ben Abdallah-Kolsi
authors.affiliation: Higher Institute of Biotechnology of Sfax (ISBS), Tunisia.|Animal Ecophysiology Laboratory, Faculty of Sciences, Sfax, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Hamden
authors.affiliation: Animal Ecophysiology Laboratory, Faculty of Sciences, Sfax, Tunisia.
authors.forename: Abdelfattah El
authors.initials: AE
authors.lastname: Feki
authors.affiliation: Biochemistry Laboratory, CHU H. Bourguiba, Sfax, Tunisia.
authors.forename: Khansa
authors.initials: K
authors.lastname: Chaabouni
authors.affiliation: Biochemistry Laboratory, CHU H. Bourguiba, Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Makni-Ayadi
authors.affiliation: Anatomical Pathology and Cytology Laboratory, Ibn Nafis, Sfax, Tunisia.
authors.forename: Fahima
authors.initials: F
authors.lastname: Sallemi
authors.affiliation: Unit "Enzymes and Bioconversion", National School of Engineers of Sfax (ENIS), Sfax, Tunisia.
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouze-Chaabouni
authors.affiliation: Unit "Enzymes and Bioconversion", National School of Engineers of Sfax (ENIS), Sfax, Tunisia.|Higher Institute of Biotechnology of Sfax (ISBS), Tunisia.
authors.forename: Dhouha
authors.initials: D
authors.lastname: Ghribi-Aydi
mesh_terms: D000496:Alloxan; D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D000960:Hypolipidemic Agents / Q000627:therapeutic use*; D007677:Kidney Function Tests; D008049:Lipase / Q000097:blood; D008055:Lipids / Q000097:blood; D008111:Liver Function Tests; D008297:Male; D010186:Pancreatic Function Tests; D051381:Rats; D017208:Rats, Wistar; D013501:Surface-Active Agents / Q000627:therapeutic use*; D000516:alpha-Amylases / Q000097:blood
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D008055:Lipids; D013501:Surface-Active Agents; D000496:Alloxan; D008049:Lipase; D000516:alpha-Amylases
keywords: Bacillus subtilis; alloxan; antilipidemic effect; biosurfactant; hypoglycemic activity; liver-kidney functions; pancreas