title: Evidence for the presence of clofazimine and its distribution in the healthy mouse brain.
abstract: This is the first report of clofazimine (CFZ) penetration and distribution in normal mouse brain. Mice were administered 25 mg/kg CFZ or 100 mg/kg CFZ orally, daily for 2 weeks. Animals were sacrificed and blood and brain tissues were harvested. Liquid chromatography tandem mass spectrometry showed high concentrations of CFZ in homogenized brain, with 100 mg/kg dose having significantly higher concentration than 25 mg/kg. Matrix-assisted laser desorption/ionization spectrometric imaging of brain sections showed widespread tissue distribution of CFZ. Our results show dose dependent localization in brain.
authors.affiliation: Catalysis and Peptide Research Unit, School of Pharmacy and Pharmacology, University of KwaZulu-Natal, Westville Campus, Durban, South Africa.
authors.forename: Sooraj
authors.initials: S
authors.lastname: Baijnath
authors.forename: Suhashni
authors.initials: S
authors.lastname: Naiker
authors.forename: Adeola
authors.initials: A
authors.lastname: Shobo
authors.forename: Chivonne
authors.initials: C
authors.lastname: Moodley
authors.forename: John
authors.initials: J
authors.lastname: Adamson
authors.forename: Bongani
authors.initials: B
authors.lastname: Ngcobo
authors.forename: Linda A
authors.initials: LA
authors.lastname: Bester
authors.forename: Sanil
authors.initials: S
authors.lastname: Singh
authors.forename: Hendrik G
authors.initials: HG
authors.lastname: Kruger
authors.forename: Tricia
authors.initials: T
authors.lastname: Naicker
authors.forename: Thavendran
authors.initials: T
authors.lastname: Govender
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002991:Clofazimine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000893:Anti-Inflammatory Agents; D002991:Clofazimine