title: Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli.
abstract: The production of bacterial polyesters, polyhydroxyalkanoates (PHAs), has been improved by several rational approaches such as overexpression and/or engineering of the enzymes directly related to PHA biosynthetic pathways. In this study, a new approach at transcription level has been applied to a new category of the copolymer of lactate (LA) and 3-hydroxybutyrate (3HB), P(LA-co-3HB). When the 4 disrupting mutants of sigma factors in Escherichia coli, rpoN, rpoS, fliA, fecI, were used as platforms for production of P(LA-co-3HB), increases in the production level and LA fraction of the copolymer were observed for the mutant strain with rpoN disruption. These positive impacts on the polymer production were caused in an "indirect manner" via changes in the multiple genes governed by RpoN. A genome-wide engineering by sigma factors would be a versatile approach for the production of value-added products of interest and available for combination with the other beneficial tools.
authors.affiliation: a Division of Biotechnology and Macromolecular Chemistry; Graduate School of Engineering; Hokkaido University ; Kita-ku, Sapporo , Japan.|b CREST; JST; Sanbancho ; Chiyoda-ku, Tokyo , Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kadoya
authors.affiliation: a Division of Biotechnology and Macromolecular Chemistry; Graduate School of Engineering; Hokkaido University ; Kita-ku, Sapporo , Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kodama
authors.affiliation: a Division of Biotechnology and Macromolecular Chemistry; Graduate School of Engineering; Hokkaido University ; Kita-ku, Sapporo , Japan.
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: a Division of Biotechnology and Macromolecular Chemistry; Graduate School of Engineering; Hokkaido University ; Kita-ku, Sapporo , Japan.|b CREST; JST; Sanbancho ; Chiyoda-ku, Tokyo , Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D019344:Lactic Acid / Q000302:isolation & purification / Q000378:metabolism*; D011091:Polyesters; D054813:Polyhydroxyalkanoates / Q000302:isolation & purification / Q000378:metabolism*; D011108:Polymers / Q000302:isolation & purification / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011108:Polymers; C527350:RpoN protein, Burkholderia cenocepacia; D012808:Sigma Factor; D019344:Lactic Acid; C033616:poly(lactide); D020155:3-Hydroxybutyric Acid
keywords: Escherichia coli; biobased plastic; poly(lactic acid); polyhydroxyalkanoate; polyhydroxybutyrate; sigma factor