title: Chemiluminescence detection of a protein through the aptamer-controlled catalysis of a porphyrin probe.
abstract: Sensitive and selective protein detection based on the aptamer-controlled noncovalent porphyrin probe self-assembly is reported for the first time. Vascular endothelial growth factor (VEGF) is a predominant biomarker in cancer angiogenesis. In this work, a positively charged porphyrin probe, manganese(III) meso-tetrakis(N-methylpyridinum-4-yl)porphyrin (Mn-PyP), was prepared. Using it as a catalyst, a label-free chemiluminescence (CL) turn-on approach for sensitive VEGF detection is developed. Mn-PyP could catalyze the luminol CL reaction. The VEGF aptamer could induce aggregation of Mn-PyP. As a result, the Mn-PyP-catalyzed CL reaction is efficiently suppressed. Upon the addition of VEGF, the specific binding of VEGF to the aptamer weakens the interactions between the aptamer and Mn-PyP. The Mn-PyP monomers are released, and a turn-on CL signal is thus detected. Our method is quite sensitive; 50 pM of VEGF could be easily detected. It is also very selective against other proteins. Our assay provides an aptamer-based efficient way for protein quantification.
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.|‡University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.|‡University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
authors.forename: Huipeng
authors.initials: H
authors.lastname: Zhou
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: †State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.|‡University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D057230:Limit of Detection; D049449:Luminescence; D008345:Manganese / Q000737:chemistry*; D015394:Molecular Structure; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011166:Porphyrins; D011506:Proteins; D042461:Vascular Endothelial Growth Factor A; D008345:Manganese