title: The Arabidopsis TAC Position Viewer: a high-resolution map of transformation-competent artificial chromosome (TAC) clones aligned with the Arabidopsis thaliana Columbia-0 genome.
abstract: We present a high-resolution map of genomic transformation-competent artificial chromosome (TAC) clones extending over all Arabidopsis thaliana (Arabidopsis) chromosomes. The Arabidopsis genomic TAC clones have been valuable genetic tools. Previously, we constructed an Arabidopsis genomic TAC library consisting of more than 10,000 TAC clones harboring large genomic DNA fragments extending over the whole Arabidopsis genome. Here, we determined 13,577 end sequences from 6987 Arabidopsis TAC clones and mapped 5937 TAC clones to precise locations, covering approximately 90% of the Arabidopsis chromosomes. We present the large-scale data set of TAC clones with high-resolution mapping information as a Java application tool, the Arabidopsis TAC Position Viewer, which provides ready-to-go transformable genomic DNA clones corresponding to certain loci on Arabidopsis chromosomes. The TAC clone resources will accelerate genomic DNA cloning, positional walking, complementation of mutants and DNA transformation for heterologous gene expression.
authors.affiliation: Honda Research Institute Japan Co., Ltd., 8-1 Honcho, Wako-shi, Saitama, 351-0188, Japan.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Suda
authors.affiliation: Laboratory of Genetic Engineering, South China Agricultural University, Gungzhou, 510642, China.|State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Gungzhou, 510642, China.
authors.forename: Yao-Guang
authors.initials: YG
authors.lastname: Liu
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.|Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, 980-8577, Japan.
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.affiliation: Bioinformatics Laboratory, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kanagawa, Kawasaki, 214-8571, Japan.
authors.forename: Yukino
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Bioinformatics Laboratory, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kanagawa, Kawasaki, 214-8571, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yokoyama
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.|Bioinformatics Laboratory, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kanagawa, Kawasaki, 214-8571, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hanano
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Takita
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Sakurai
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Takakazu
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Bioinformatics Laboratory, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kanagawa, Kawasaki, 214-8571, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yano
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics; D022201:Chromosomes, Artificial; D054629:Genome, Mitochondrial; D018745:Genome, Plant; D015698:Genomic Library; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000379:methods*; D012984:Software
keywords: Arabidopsis TAC clones; Arabidopsis TAC library; Arabidopsis genome; Arabidopsis thaliana; GBrowse; bioinformatics; genomics; transformation-competent artificial chromosome