title: Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization.
abstract: The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae[1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisiae. The fluorescence of fusion protein Mgt05196p-GFP expressing strain was located on the cell surface demonstrated that the heterogenous transporter Mgt05196p was targeted to the plasma membrane of S. cerevisiae. The expressing of Mgt05196p in the hxt null S. cerevisiae endowed the strain with the glucose and d-xylose absorption capacity, as well as expressing the native d-xylose transporter Gal2p. The transmembrane domains of Mgt05196p were predicted and compared with the XylEp, whose crystal structure was revealed. And then, the homologous modeling of Mgt05196p was built basing on the XylEp to find out the crucial amino acid residues for sugars binding and transport.
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China ; College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Daizong Street 61, Taian 271018, PR China.
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
authors.affiliation: Environment Research Institute, Shandong University, Shan Da Nan Road 27, Jinan 250100, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Jiao
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.affiliation: Environment Research Institute, Shandong University, Shan Da Nan Road 27, Jinan 250100, PR China.
authors.forename: Qingzhu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen