title: Simultaneous detection of 33 Streptococcus suis serotypes using the luminex xTAG® assay™.
abstract: We report the development and evaluation of a serotype-specific xTAG luminex assay (SSA) that allows detection of the 33 serotypes of Streptococcus suis (S. suis). This assay is based on wzy gene targets directly involved in the cps biosynthesis and can be completed 40 min post-PCR amplification. The assay correctly and specifically identified the serotype of all 209 isolates tested, in comparison with two serotyping multiplex PCR methods previously developed. The sensitivity was higher than that of the previously described methods. The SSA system described here provides an easy-to-use, high-throughput system for rapid detection of S. suis serotypes.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Bai
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Ji
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montréal, Québec, Canada.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. Electronic address: zhenghan@icdc.cn.
authors.forename: Han
authors.initials: H
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D057230:Limit of Detection; D058889:Molecular Typing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction; D012703:Serotyping / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D013552:Swine
keywords: Luminex; Serotype; Streptococcus suis; wzy