title: In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
abstract: Microglia, astrocytes, and neurons, which have important functions in the central nervous system (CNS), communicate mutually to generate a signal through secreted proteins or small molecules, but many of which have not been identified. Because establishing a reference for the secreted proteins from CNS cells could be invaluable in examining cell-to-cell communication in the brain, we analyzed the secretome of three murine CNS cell lines without prefractionation by high-resolution mass spectrometry. In this study, 2795 proteins were identified from conditioned media of the three cell lines, and 2125 proteins were annotated as secreted proteins by bioinformatics analysis. Further, approximately 500 secreted proteins were quantifiable as differentially expressed proteins by label-free quantitation. As a result, our secretome references are useful datasets for the future study of neuronal diseases. All MS data have been deposited in the ProteomeXchange with identifier PXD001597 (http://proteomecentral.proteomexchange.org/dataset/PXD001597).
authors.affiliation: Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Woo
authors.affiliation: Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.|Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
authors.forename: Dohyun
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Joonho
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Physiology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Sang Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.|Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.|Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002490:Central Nervous System / Q000166:cytology* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D051379:Mice; D017628:Microglia / Q000737:chemistry / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D017077:Culture Media, Conditioned; D011506:Proteins
keywords: Astrocyte; Microglia; Neuron; Secretome; Technology