title: JC polyomavirus nephropathy confirmed by using an in-house polymerase chain reaction method.
abstract: We report the case of an isolated JC virus (JCV) infection, without co-infection by polyoma BK virus (BKV), associated with nephropathy 4 years after kidney transplantation. Clinical suspicion followed the observation of a decrease in estimated glomerular filtration rate (eGFR) and a renal allograft biopsy revealing polyomavirus-associated tubulointerstitial nephritis and positivity for SV40. An in-house real-time polymerase chain reaction assay, targeting the presence of JCV and the absence of BKV in biopsy tissue, confirmed diagnosis. Thirteen months after diagnosis, and following therapeutic measures, eGFR remains stable.
authors.affiliation: Department of Nephrology, Médio Tejo Hospitalar Center, Torres Novas, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Querido
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Jorge
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: H
authors.initials: H
authors.lastname: Sousa
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Birne
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Matias
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Weigert
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: T
authors.initials: T
authors.lastname: Adragão
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Bruges
authors.affiliation: Department of Pathology, Lisbon Ocidental Hospitalar Center, Lisbon, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Ramos
authors.affiliation: Department of Clinical Pathology, Lisbon Central Hospitalar Center, Lisbon, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: Nova Medical School, Lisbon, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Paixão
authors.affiliation: Clinical Microbiology and Public Health Laboratory, Public Health England, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: M D
authors.initials: MD
authors.lastname: Curran
authors.affiliation: Department of Nephrology, Santa Cruz Hospital, Carnaxide, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Machado
mesh_terms: D006801:Humans; D007577:JC Virus / Q000302:isolation & purification*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D027601:Polyomavirus Infections / Q000175:diagnosis* / Q000209:etiology; D011183:Postoperative Complications / Q000175:diagnosis*; D060888:Real-Time Polymerase Chain Reaction; D051437:Renal Insufficiency / Q000175:diagnosis* / Q000209:etiology; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000209:etiology
keywords: JC virus; PCR; PVAN; nephropathy; renal transplant; viremia; viruria