title: Isolation and Identification of Arcobacter spp. by Multiplex PCR from Water Sources in Kars Region.
abstract: This study was conducted to determine the prevalence of Arcobacter spp. in various water sources of stream, creek, pond, and drinking water in Kars and surrounding areas. A total of 113 water samples including 19 samples from creeks, 49 from streams, 10 from ponds, and 35 from drinking water samples collected from different regions were examined for presence of Arcobacter spp. by cultural methods. Arcobacter spp. were isolated from 14 (12.38 %) samples including 5 (26.31 %) creek and 9 (18.36 %) stream water samples and all were identified as Arcobacter butzleri by multiplex PCR. No agent was isolated from pond and drinking water samples. The results of this study demonstrated that creek and stream waters are contaminated by this agent showing high potential risk of Arcobacter species to be transmitted to humans and animals and in the contamination of food. It is concluded that water sources should also be considered as a factor not only carrying agents but also as a primary source of the infection.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey. elita_3609@hotmail.com.
authors.forename: Elif
authors.initials: E
authors.lastname: Çelik
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, 17000, Çanakkale, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ünver
mesh_terms: D020640:Arcobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial; D005819:Genetic Markers; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D012621:Seasons; D014421:Turkey; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers