title: The Positive Predictive Value of Lyme Elisa for the Diagnosis of Lyme Disease in Children.
abstract: By using a Lyme enzyme-linked immunosorbent assay (ELISA), we demonstrated that high ELISA index values are strongly predictive of Lyme disease. In children with clinical presentations consistent with Lyme disease, ELISA index values ≥3.0 had a positive predictive value of 99.4% (95% confidence interval: 98.1-99.8%) for Lyme disease, making a supplemental Western immunoblot potentially unnecessary.
authors.affiliation: From the *Division of Emergency Medicine, Boston Children's Hospital, Boston, Massachusetts; †Infectious Diseases Diagnostic Laboratory, Boston Children's Hospital and Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts; ‡Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts; and Divisions of §Pediatric Infectious Diseases and ¶General Internal Medicine, Duke University School of Medicine, Durham, North Carolina.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Lipsett
authors.forename: Nira R
authors.initials: NR
authors.lastname: Pollock
authors.forename: John A
authors.initials: JA
authors.lastname: Branda
authors.forename: Caroline D
authors.initials: CD
authors.lastname: Gordon
authors.forename: Catherine R
authors.initials: CR
authors.lastname: Gordon
authors.forename: Paul M
authors.initials: PM
authors.lastname: Lantos
authors.forename: Lise E
authors.initials: LE
authors.lastname: Nigrovic
mesh_terms: D000293:Adolescent; D002648:Child; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis*; D008297:Male; D011237:Predictive Value of Tests; D012189:Retrospective Studies