title: Octreotide protects ovary against ischemia-reperfusion injury in rats: Evaluation of histological and biochemical parameters.
abstract: OBJECTIVE
  This study investigated the efficacy of octreotide for prevention of ischemia-reperfusion injury in rat ovary.
  
  
  METHODS
  Thirty-two adult female rats were included. Rats were divided into five groups: in the sham group, the abdominal wall was only opened and closed; in the torsion group, ischemia was induced for 3 h using a torsion model involving atraumatic vascular clips; in the torsion/octreotide group, rats were given 100 µg/kg i.p. octreotide 30 min before torsion was induced; in the torsion/detorsion group, rats underwent 3 h ischemia-3 h reperfusion; in the torsion/detorsion/octreotide group, rats underwent 3 h ischemia followed by 100 µg/kg octreotide i.p. 30 min prior to 3 h reperfusion. Ovarian tissue damage was scored on histopathology. Ovarian tissue malondialdehyde and plasma pentraxin 3 were measured biochemically.
  
  
  RESULTS
  In comparison with the sham group, both the torsion and torsion/detorsion groups had significantly higher scores for follicular degeneration, vascular congestion, edema, hemorrhage and leukocyte infiltration. Octreotide significantly decreased these scores in both groups. Ovarian malondialdehyde and plasma pentraxin 3 were significantly higher both in the torsion and torsion/detorsion groups compared with the sham group. Octreotide also decreased these levels significantly both in the torsion/octreotide and torsion/detorsion/octreotide groups.
  
  
  CONCLUSIONS
  Octreotide ameliorated the potential side-effects of ovarian ischemia-reperfusion injury in a rat model.
authors.affiliation: Department of Obstetrics and Gynecology, Ege University, Izmir, Turkey.
authors.forename: Nuri
authors.initials: N
authors.lastname: Yildirim
authors.affiliation: Department of Histology and Embryology, Ege University, Izmir, Turkey.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Yigitturk
authors.affiliation: Department of Obstetrics and Gynecology, Ataturk Training and Research Hospital, Izmir, Turkey.
authors.forename: Alkim Gulsah
authors.initials: AG
authors.lastname: Sahingoz Yildirim
authors.affiliation: Department of Obstetrics and Gynecology, Ege University, Izmir, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Akdemir
authors.affiliation: Department of Obstetrics and Gynecology, Ege University, Izmir, Turkey.
authors.forename: Orkun
authors.initials: O
authors.lastname: İlgen
authors.affiliation: Department of Obstetrics and Gynecology, Ege University, Izmir, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Yeniel
authors.affiliation: Department of Obstetrics and Gynecology, Ege University, Izmir, Turkey.
authors.forename: Mete
authors.initials: M
authors.lastname: Ergenoglu
authors.affiliation: Department of Physiology, Bilim University, Istanbul, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbas
mesh_terms: D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use*; D005260:Female; D015282:Octreotide / Q000627:therapeutic use*; D010049:Ovarian Diseases / Q000473:pathology / Q000517:prevention & control*; D010053:Ovary / Q000473:pathology; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D015282:Octreotide
keywords: ischemia-reperfusion; octreotide; ovary; rat