title: Transepithelial transport of milk derived bioactive peptide VLPVPQK.
abstract: The transepithelial transport of an antioxidative and ACE inhibitory peptide, VLPVPQK (named peptide C) derived from casein hydrolysates was investigated along with extensively studied opioid peptide β-casomorphin using a human intestinal cell (Caco-2) monolayer. The susceptibility to the brush-border peptidases and route of transepithelial transport were observed to be the primary factors influencing the transport of these peptides. The apical to basal transport mechanism was studied using bradykinin as control as it shows resistance to cellular peptidases and its route of transepithelial transport had been established. VLPVPQK and BCM 5 were hydrolyzed by cellular peptidases while bradykinin was found intact. The transport of VLPVPQK (1.0%) was found to be relatively much higher than BCM 5 (0.03%) and bradykinin (0.1%). Interestingly the effect of some inhibitors on the transport of VLPVPQK suggested involvement of PepT1 like transporters/SOPT2 while BCM 5, its hydrolytic product and bradykinin were suggested to be transported mainly via the intracellular transcytosis pathway.
authors.affiliation: Animal Biochemistry Division, NDRI, Karnal, Haryana, India. Electronic address: rishikavij@gmail.com.
authors.forename: Rishika
authors.initials: R
authors.lastname: Vij
authors.affiliation: Animal Biochemistry Division, NDRI, Karnal, Haryana, India. Electronic address: bioreddy.srinu89@gmail.com.
authors.forename: Srinu
authors.initials: S
authors.lastname: Reddi
authors.affiliation: Animal Biochemistry Division, NDRI, Karnal, Haryana, India. Electronic address: suman_ndri@yahoo.com.
authors.forename: Suman
authors.initials: S
authors.lastname: Kapila
authors.affiliation: Animal Biochemistry Division, NDRI, Karnal, Haryana, India. Electronic address: rkapila69@rediffmail.com.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Kapila
mesh_terms: D000818:Animals; D000975:Antioxidants; D018938:Caco-2 Cells / Q000737:chemistry*; D002417:Cattle; D006801:Humans; D008892:Milk / Q000737:chemistry*; D057705:Transendothelial and Transepithelial Migration
chemical_list: D000975:Antioxidants
keywords: Antihypertensive; Antioxidative; Caco-2 cells; Peptide; Transepithelial transport