title: [Extracts from Testudinis Carapacis et Plastri Regulates Expression of ID1 in Mscs].
abstract: OBJECTIVE
  To explore BMP4 affecting the Extracts from Testudinis Carapacis et Plastri (PTE) stimulating proliferation of MSCs and the mechanism.
  
  
  METHODS
  Cotransfected PGL3-IDI and pEGFP-BMP4 of 0, 0. 1,0. 3, 0. 5 and 1 µg/mL respectively using the calcium phosphate co-precipitation method in rat MSCs. One of transfected cells were divided into control group and PTE group. PTE group was stimulated by PTE of 30 µ/L for 36 h, while control group was not. Collected cells using lucifease activity measurement to detect the activity of ID. Then 0. 3 µg/mL pEGFP-BMP4 was chose to cotransfect. MSCs was divided into control group, PTE group, BMP4 group, BMP4 + PTE group. BMP4 and BMP4 + PTE group were cotransfected with PGL3-ID1 and pEGFP-BMP4 but control or PTE groups were not. PTE and BMP4 + PTE groups were stimulated by PTE of 30 µg/mL for 36 h but the either two groups were not. The activities of ID1, BMP4 and RARα were detected using RT-PCR.
  
  
  RESULTS
  The expressions of ID1, BMP4 and RARa rose in PTE group. The expression of BMP4 and RARα rose while IDI decreased in BMP4 groups. BMP4, ID1 and RARα decreased remarkable in BMP4 + PTE group comparing with BMP4 group.
  
  
  CONCLUSIONS
  PTE promotes the proliferation of MSCs, it also regulates the expression of BMP4 to prevent excessive proliferation of MSCs.
authors.forename: Hai-ling
authors.initials: HL
authors.lastname: Zhang
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Mao-sheng
authors.initials: MS
authors.lastname: Ye
authors.forename: Jian-zhen
authors.initials: JZ
authors.lastname: Huang
authors.forename: Dong-feng
authors.initials: DF
authors.lastname: Chen
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D001688:Biological Products / Q000737:chemistry; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D049109:Cell Proliferation; D051798:Inhibitor of Differentiation Protein 1 / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D000072482:Retinoic Acid Receptor alpha; D014162:Transfection; D014426:Turtles
chemical_list: D001688:Biological Products; C525811:Bmp4 protein, rat; D055415:Bone Morphogenetic Protein 4; C497454:ID1 protein, rat; D051798:Inhibitor of Differentiation Protein 1; C000607073:Rara protein, rat; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha