title: Recombinant disintegrin domain of human ADAM9 inhibits migration and invasion of DU145 prostate tumor cells.
abstract: One of the most important features of malignant cells is their capacity to invade adjacent tissues and metastasize to distant organs. This process involves the creation, by tumor and stroma cells, of a specific microenvironment, suitable for proliferation, migration and invasion of tumor cells. The ADAM family of proteins has been involved in these processes. This work aimed to investigate the role of the recombinant disintegrin domain of the human ADAM9 (rADAM9D) on the adhesive and mobility properties of DU145 prostate tumor cells. rADAM9D was able to support DU145 cell adhesion, inhibit the migration of DU145 cells, as well as the invasion of this cell line through matrigel in vitro. Overall this work demonstrates that rADAM9D induces specific cellular migratory properties when compared with different constructs having additional domains, specially those of metalloproteinase and cysteine-rich domains. Furthermore, we showed that rADAM9D was able to inhibit cell adhesion, migration and invasion mainly through interacting with α6β1 in DU145 tumor cell line. These results may contribute to the development of new therapeutic strategies for prostate cancer.
authors.affiliation: a Departamento de Ciências Fisiológicas; Universidade Federal de São Carlos; São Carlos , Brazil.
authors.forename: Ana Carolina Baptista Moreno
authors.initials: AC
authors.lastname: Martin
authors.forename: Ana Carolina Ferreira
authors.initials: AC
authors.lastname: Cardoso
authors.forename: Heloisa Sobreiro
authors.initials: HS
authors.lastname: Selistre-de-Araujo
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Cominetti
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D019483:Disintegrins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D019483:Disintegrins; D008565:Membrane Proteins; D051722:ADAM Proteins; C098339:ADAM9 protein, human
keywords: ADAM; cell adhesion; disintegrin; integrin; invasion; metastasis; prostate cancer