title: A novel approach to arthritis treatment based on resveratrol and curcumin co-encapsulated in lipid-core nanocapsules: In vivo studies.
abstract: Resveratrol and curcumin are two natural polyphenols extensively used due to their remarkable anti-inflammatory activity. The present work presents an inedited study of the in vivo antioedematogenic activity of these polyphenols co-encapsulated in lipid-core nanocapsules on Complete Freund's adjuvant (CFA)-induced arthritis in rats. Lipid-core nanocapsules were prepared by interfacial deposition of preformed polymer. Animals received a single subplantar injection of CFA in the right paw. Fourteen days after arthritis induction, they were treated with resveratrol, curcumin, or both in solution or loaded in lipid-core nanocapsules (1.75 mg/kg/twice daily, i.p.), for 8 days. At the doses used, the polyphenols in solution were not able to decrease paw oedema. However, nanoencapsulation improved the antioedematogenic activity of polyphenols at the same doses. In addition, the treatment with co-encapsulated polyphenols showed the most pronounced effects, where an inhibition of 37-55% was observed between day 16 and 22 after arthritis induction. This treatment minimized most of the histological changes observed, like fibrosis in synovial tissue, cartilage and bone loss. In addition, unlike conventionally arthritis treatment, resveratrol and curcumin co-encapsulated in lipid-core nanocapsules did not alter important hepatic biochemical markers (ALP, AST, and ALT). In conclusion, the strategy of co-encapsulating resveratrol and curcumin in lipid-core nanocapsules improves their efficacy as oedematogenic agents, with no evidence of hepatotoxic effects. This is a promising strategy for the development of new schemes for treatment of chronic inflammation diseases, like arthritis.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Karine
authors.initials: K
authors.lastname: Coradini
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Rossana B
authors.initials: RB
authors.lastname: Friedrich
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Francisco N
authors.initials: FN
authors.lastname: Fonseca
authors.affiliation: Laboratório de Morfofisiologia Experimental, Departamento de Morfologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Marina S
authors.initials: MS
authors.lastname: Vencato
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Andrade
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Oliveira
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Battistel
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Silvia S
authors.initials: SS
authors.lastname: Guterres
authors.affiliation: Laboratório de Morfofisiologia Experimental, Departamento de Morfologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Maria Izabel U M
authors.initials: MI
authors.lastname: da Rocha
authors.affiliation: Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Adriana R
authors.initials: AR
authors.lastname: Pohlmann
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: ruy.beck@ufrgs.br.
authors.forename: Ruy C R
authors.initials: RC
authors.lastname: Beck
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000473:pathology; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D033023:Foot Joints / Q000473:pathology; D056604:Grape Seed Extract / Q000737:chemistry; D006601:Hexoses / Q000737:chemistry; D007274:Injections, Intraperitoneal; D008297:Male; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D011091:Polyesters / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D004337:Drug Carriers; D056604:Grape Seed Extract; D006601:Hexoses; D053769:Nanocapsules; D011091:Polyesters; D011136:Polysorbates; D013267:Stilbenes; C016240:polycaprolactone; D003474:Curcumin; C009298:sorbitan monostearate; D000077185:Resveratrol
keywords: Antioedematogenic; Arthritis; CFA; Curcumin; Lipid-core nanocapsules; Resveratrol