title: Altered expression of monocyte and dendritic cell membrane-associated antigens following coronary angiography.
abstract: BACKGROUND
  Coronary angiography is able to induce a systemic inflammatory response. We hypothesised that this procedure may affect monocyte and dendritic cell count and membrane-associated antigen expression.
  
  
  METHODS
  Blood samples were obtained before and immediately after coronary angiography in twenty patients with stable angina pectoris. Cell enumeration and antigen expression levels were evaluated by flow cytometry. Plasma levels of soluble CD14 and interleukin-6 were quantified by ELISA.
  
  
  RESULTS
  The absolute and relative numbers of circulating monocytes (Mon1, Mon2 and Mon3 subsets) and dendritic cells (myeloid and plasmacytoid subsets) were not significantly different pre-versus post-angiography. Expression of CD14 on Mon1 and Mon2 decreased significantly by 12.01% (P = 0.002) and 13.01% (P=0.012), respectively. CD16 expression on Mon2 (+10.53%; P=0.017) and Mon3 (+12.58%; P<0.001) increased. CD45 expressed by monocytic and dendritic cells was lowered (-5.80% and P = 0.001, -11.49% and P < 0.001, respectively). The level of plasma IL-6 decreased significantly (P = 0.002). The reduction in sCD14 was not significant (P = 0.054).
  
  
  CONCLUSIONS
  Coronary angiography leads to changes in surface expression of CD14, CD16 and CD45. These findings underline the importance of blood collection prior to the angiographic procedure when aiming to study the functional analysis of monocyte and dendritic cell numbers by flow cytometry.
authors.forename: Sam C
authors.initials: SC
authors.lastname: Latet
authors.forename: Paul L
authors.initials: PL
authors.lastname: Van Herck
authors.forename: Amaryllis H
authors.initials: AH
authors.lastname: Van Craenenbroeck
authors.forename: Katrijn
authors.initials: K
authors.lastname: Van Ackeren
authors.forename: Emeline M
authors.initials: EM
authors.lastname: Van Craenenbroeck
authors.forename: Christiaan J
authors.initials: CJ
authors.lastname: Vrints
authors.forename: Vicky Y
authors.initials: VY
authors.lastname: Hoymans
mesh_terms: D000368:Aged; D002452:Cell Count; D002462:Cell Membrane / Q000737:chemistry; D017023:Coronary Angiography; D003713:Dendritic Cells / Q000737:chemistry*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood*; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D008297:Male; D009000:Monocytes / Q000737:chemistry*; D017452:Receptors, IgG / Q000097:blood*
chemical_list: D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D017493:Leukocyte Common Antigens