title: Phlorotannins suppress adipogenesis in pre-adipocytes while enhancing osteoblastogenesis in pre-osteoblasts.
abstract: Osteoporosis, a prevalent bone disease in an aging population, is considered to be closely related to osteoblastogenesis and adipogenesis. As a part of an ongoing trend to develop natural substances that attenuate osteoporotic conditions, edible brown algae E. cava and its bioactive constituents were tested for their effects on adipogenic differentiation in 3T3-L1 fibroblasts and osteoblast differentiation in MC3T3-E1 pre-osteoblasts. Following an activity-based isolation, three phlorotannin derivatives, triphlorethol-A (1), eckol (2) and dieckol (3), were isolated. Anti-adipogenesis effect of phlorotannins at the concentration of 20 µM was observed by reduced lipid accumulation and the suppressed expression of adipogenic differentiation markers. In addition, isolated phlorotannins successfully enhanced the osteoblast differentiation as indicated by increased alkaline phosphatase activity along with raised levels of osteoblastogenesis indicators and intracellular calcification at the concentration of 20 µM. In conclusion, E. cava is suggested as a source for functional food ingredients, especially phlorotannin derivatives that can be utilized for extenuating osteoporosis and obesity.
authors.affiliation: Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Pusan, 617-736, Republic of Korea.
authors.forename: Fatih
authors.initials: F
authors.lastname: Karadeniz
authors.affiliation: Department of Organic Material Science and Engineering, Pusan National University, Pusan, 609-735, Republic of Korea.
authors.forename: Byul-Nim
authors.initials: BN
authors.lastname: Ahn
authors.affiliation: Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Pusan, 617-736, Republic of Korea.|Department of Food and Nutrition, College of Medical and Life Science, Silla University, Pusan, 617-736, Republic of Korea.
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
authors.affiliation: Division of Marine Bioscience; Ocean Science & Technology School, Korea Maritime and Ocean University, Pusan, 606-791, Republic of Korea.
authors.forename: Youngwan
authors.initials: Y
authors.lastname: Seo
authors.affiliation: Food and Safety Research Center, National Fisheries Research and Development Institute, Pusan, 619-705, Republic of Korea.
authors.forename: Mi-Soon
authors.initials: MS
authors.lastname: Jang
authors.affiliation: Food and Safety Research Center, National Fisheries Research and Development Institute, Pusan, 619-705, Republic of Korea.
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Nam
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Pusan, 617-736, Republic of Korea.
authors.forename: Mihyang
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Bioscience, Graduate School, Silla University, Pusan, 617-736, Republic of Korea.
authors.forename: Sang-Hyeon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Pusan, 617-736, Republic of Korea. cskong@silla.ac.kr.
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D000459:Phaeophyceae; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D013634:Tannins
keywords: Adipogenesis; E. cava; Osteoblastogenesis; Osteoporosis; Phlorotannin