title: Characterization of miRNA processing machinery in the embryonic chick lung.
abstract: Lung development is a very complex process that relies on the interaction of several signaling pathways that are controlled by precise regulatory mechanisms. Recently, microRNAs (miRNAs), small non-coding regulatory RNAs, have emerged as new players involved in gene expression regulation controlling several biological processes, such as cellular differentiation, apoptosis and organogenesis, in both developmental and disease processes. Failure to correctly express some specific miRNAs or a component of their biosynthetic machinery during embryonic development is disastrous, resulting in severe abnormalities. Several miRNAs have already been identified as modulators of lung development. Regarding the spatial distribution of the processing machinery of miRNAs, only two of its members (dicer1 and argonaute) have been characterized. The present work characterizes the expression pattern of drosha, dgcr8, exportin-5 and dicer1 in early stages of the embryonic chick lung by whole mount in situ hybridization and cross-section analysis. Overall, these genes are co-expressed in dorsal and distal mesenchyme and also in growing epithelial regions. The expression pattern of miRNA processing machinery supports the previously recognized regulatory role of this mechanism in epithelial and mesenchymal morphogenesis.
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal. rutemoura@ecsaude.uminho.pt.|ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal. rutemoura@ecsaude.uminho.pt.|Biology Department, School of Sciences, University of Minho, Braga, Portugal. rutemoura@ecsaude.uminho.pt.
authors.forename: Rute Silva
authors.initials: RS
authors.lastname: Moura
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.|ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Vaz-Cunha
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.|ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Silva-Gonçalves
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.|ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.|Department of Pediatric Surgery, Hospital de Braga, Braga, Portugal.
authors.forename: Jorge
authors.initials: J
authors.lastname: Correia-Pinto
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D008168:Lung / Q000196:embryology* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*
chemical_list: D030161:Avian Proteins; D035683:MicroRNAs
keywords: Chick lung; dgcr8; dicer1; drosha; exportin-5