title: Efficient differentiation of neural stem cells induced by the rat bone marrow stromal cells.
abstract: Neural stem cells (NSCs) are valuable self-renewing cells that can maintain the capacity to differentiate into specific brain cell types. NSCs may repair and even replace the brain tissue, and ultimatley promoting the central nervous system regeneration. Therefore, it is important, for scientists and pjysicians, to study the method for efficient culture and differentiation of NSCs. Our previous study demonstrated that Bone Marrow Stromal Cells (BMSCs) can directly regulate the differentiation of NSCs into neurons, and soluble molecules excreted by BMSCs played a key role in this process. Hereby, we further identified the BMSCs-induced neurons could form the synapses, convey dopamine and express voltage-depend and receptor-depend calcium channels. Moreover, the extracellular signal-regulated protein kinase ERK1/2 pathway was founded to be involved in the process of neuron differentiation and proliferation by the in vitro experiments. Finally, by using protein array, we, for the first time, found that the cytokine-induced neutrophil chemoattractant-3 (CINC-3, a small molecule cytokine) can promote the leukocytes invasion into the inflammation site, and have the ability to induce mesencephal NSCs into neurons. Consequently, these positive findings suggested that our BMSCs-induced culture system could provide a useful tool to investigate the molecular mechanisms of neural differentiation of NSCs, which may be benifical for neurodegenerative diseases in the near future.
authors.affiliation: Department of Gerontology, The First Hospital of Hebei Medical University Hebei, China ; Brain Aging and Cognitive, Neuroscience Laboratory of Hebei Province Shijiazhuang 050031, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.affiliation: Department of Gerontology, The First Hospital of Hebei Medical University Hebei, China.
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Qiu
authors.affiliation: Brain Aging and Cognitive, Neuroscience Laboratory of Hebei Province Shijiazhuang 050031, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Han
authors.affiliation: Brain Aging and Cognitive, Neuroscience Laboratory of Hebei Province Shijiazhuang 050031, China.
authors.forename: Zhong-Xia
authors.initials: ZX
authors.lastname: Zhang
authors.affiliation: Department of Gerontology, The First Hospital of Hebei Medical University Hebei, China.
authors.forename: Ci
authors.initials: C
authors.lastname: Dong
authors.affiliation: Brain Aging and Cognitive, Neuroscience Laboratory of Hebei Province Shijiazhuang 050031, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The First Hospital of Hebei Medical University Hebei, China.
authors.forename: Yan-Yong
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Hospital of Hebei Medical University Hebei, China.
authors.forename: Qing-Ying
authors.initials: QY
authors.lastname: Ma
authors.affiliation: Brain Aging and Cognitive, Neuroscience Laboratory of Hebei Province Shijiazhuang 050031, China.
authors.forename: Bao-Yong
authors.initials: BY
authors.lastname: Yan
keywords: Neural stem cells; bone marrow stromal cells; cytokines; differentiation; neurons; protein microarray analysis