title: Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
abstract: Mover, a member of the exquisitely small group of vertebrate-specific presynaptic proteins, has been discovered as an interaction partner of the scaffolding protein Bassoon, yet its function has not been elucidated. We used adeno-associated virus (AAV)-mediated shRNA expression to knock down Mover in the calyx of Held in vivo. Although spontaneous synaptic transmission remained unaffected, we found a strong increase of the evoked EPSC amplitude. The size of the readily releasable pool was unaltered, but short-term depression was accelerated and enhanced, consistent with an increase in release probability after Mover knockdown. This increase in release probability was not caused by alterations in Ca(2+) influx but rather by a higher Ca(2+) sensitivity of the release machinery, as demonstrated by presynaptic Ca(2+) uncaging. We therefore conclude that Mover expression in certain subsets of synapses negatively regulates synaptic release probability, constituting a novel mechanism to tune synaptic transmission.
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany. Electronic address: koerber@ana.uni-heidelberg.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Körber
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
authors.forename: Heinz
authors.initials: H
authors.lastname: Horstmann
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
authors.forename: Varun
authors.initials: V
authors.lastname: Venkataramani
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Herrmannsdörfer
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kremer
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Kaiser
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
authors.forename: Darius B
authors.initials: DB
authors.lastname: Schwenger
authors.affiliation: European Neuroscience Institute, Grisebachstrasse 5, 37077 Göttingen, Germany.
authors.forename: Saheeb
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: European Neuroscience Institute, Grisebachstrasse 5, 37077 Göttingen, Germany.
authors.forename: Camin
authors.initials: C
authors.lastname: Dean
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany; Department of Anatomy and Embryology, Centre of Anatomy, University of Göttingen, Kreuzbergring 36, 37075 Göttingen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dresbach
authors.affiliation: Institute of Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany. Electronic address: kuner@uni-heidelberg.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kuner
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D055785:Gene Knockdown Techniques / Q000379:methods; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D009924:Organ Culture Techniques; D017729:Presynaptic Terminals / Q000378:metabolism*; D011336:Probability; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009419:Nerve Tissue Proteins; C524252:Tprg1l protein, rat