title: Discovery of a novel putative atypical porcine pestivirus in pigs in the USA.
abstract: Pestiviruses are some of the most significant pathogens affecting ruminants and swine. Here, we assembled a 11 276 bp contig encoding a predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that of a recently partially characterized Rhinolophus affinis pestivirus (RaPV) and approximately 25-28 % pairwise identity to those of other pestiviruses. The virus was provisionally named atypical porcine pestivirus (APPV). Metagenomic sequencing of 182 serum samples identified four additional APPV-positive samples. Positive samples originated from five states and ELISAs using recombinant APPV Erns found cross-reactive antibodies in 94 % of a collection of porcine serum samples, suggesting widespread distribution of APPV in the US swine herd. The molecular and serological results suggest that APPV is a novel, highly divergent porcine pestivirus widely distributed in US pigs.
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, USA.|Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Hause
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, USA.|Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Collin
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, USA.|Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Lalitha
authors.initials: L
authors.lastname: Peddireddi
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Fangfeng
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, USA.|Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Hesse
authors.affiliation: Department of Veterinary Diagnostic and Population Animal Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Gauger
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD, USA.
authors.forename: Travis
authors.initials: T
authors.lastname: Clement
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, USA.|Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Anderson
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016000:Cluster Analysis; D003429:Cross Reactions; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018182:Pestivirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D044967:Serum / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014481:United States
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral