title: Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen.
abstract: Ticks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: marinelacr@hotmail.com.
authors.forename: Marinela
authors.initials: M
authors.lastname: Contreras
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: drmorenocid@probelte.es.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Moreno-Cid
authors.affiliation: Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Rua da Junqueira 100, 1349-008 Lisboa, Portugal. Electronic address: adomingos@ihmt.unl.pt.
authors.forename: Ana
authors.initials: A
authors.lastname: Domingos
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: mario.canales@probiovet.com.
authors.forename: Mario
authors.initials: M
authors.lastname: Canales
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: Iratxe.Diez@uclm.es.
authors.forename: Iratxe
authors.initials: I
authors.lastname: Díez-Delgado
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: jm.perezdelalastra@csic.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Pérez de la Lastra
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: emiliouax@hotmail.com.
authors.forename: Emilio
authors.initials: E
authors.lastname: Sánchez
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Km. 5 carretera Victoria-Mante, CP 87000 Ciudad Victoria, Tamaulipas, Mexico. Electronic address: mero840125@hotmail.com.
authors.forename: Octávio
authors.initials: O
authors.lastname: Merino
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Km. 5 carretera Victoria-Mante, CP 87000 Ciudad Victoria, Tamaulipas, Mexico. Electronic address: riglopez@uat.edu.mx.
authors.forename: Rigoberto López
authors.initials: RL
authors.lastname: Zavala
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: Nieves.Ayllon@uclm.es.
authors.forename: Nieves
authors.initials: N
authors.lastname: Ayllón
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: mariana.boadella@gmail.com.
authors.forename: Mariana
authors.initials: M
authors.lastname: Boadella
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: margaritam.villar@uclm.es.
authors.forename: Margarita
authors.initials: M
authors.lastname: Villar
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. Electronic address: Christian.Gortazar@uclm.es.
authors.forename: Christian
authors.initials: C
authors.lastname: Gortázar
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: jose_delafuente@yahoo.com.
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente
mesh_terms: D000276:Adjuvants, Immunologic; D042322:Anaplasma marginale / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002462:Cell Membrane / Q000737:chemistry; D004926:Escherichia coli; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D048494:Rhipicephalus / Q000276:immunology*; D013552:Swine; D014612:Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D000941:Antigens; D060829:Arthropod Proteins; D001425:Bacterial Outer Membrane Proteins; D014612:Vaccines; C449089:major surface protein 1a, Anaplasma; C550207:subolesin, tick
keywords: Chimera; Endotoxin; Immunology; MSP1a; Subolesin; Tick; Vaccine