title: A selective and sensitive method based on UPLC-MS/MS for quantification of momordin Ic in rat plasma: application to a pharmacokinetic study.
abstract: A selective and sensitive method was developed and validated based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This method was applied to quantify momordin Ic in rat plasma. Chromatographic separation was performed on a Hypersil GOLD HPLC C18 column (150mm×4.6mm, 5μm) using an isocratic mobile phase of acetonitrile/water (80:20, v/v) at a flow rate of 0.6mL/min. An electrospray ionization source was applied and operated in negative ion mode; selected reaction monitoring (SRM) mode was used for quantification by monitoring the precursor-to-product ion transitions of m/z 763.4→m/z 455.3 for momordin Ic, and m/z 649.4→m/z 487.3 for IS. Calibration curves showed good linearity over the range of 22.0-2200ng/mL for momordin Ic in rat plasma. The developed method was applied to a pharmacokinetic study of momordin Ic in rats after single intravenous doses at 0.52, 1.56, and 4.67mg/kg. The elimination half-life (t1/2) values were 1.22±0.39, 1.14±0.10, and 1.83±0.39h, respectively. The plasma concentration at 2min (C2min) and area under the curve (AUC) for the intravenous doses of momordin Ic were approximately dose proportional.
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun 130021, PR China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun 130021, PR China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun 130021, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun 130021, PR China. Electronic address: sxzhang1981@126.com.
authors.forename: Sixi
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019540:Area Under Curve; D027467:Bassia scoparia / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics*; D006207:Half-Life; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood; D011786:Quality Control; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C408362:momordin I (Oleanolic Acid); D009828:Oleanolic Acid
keywords: Momordin Ic; Pharmacokinetics; Rat plasma; UPLC–MS/MS