title: Physicochemical Characterization and In Vitro Cytotoxic Effect of 3-Hydroxyflavone in a Silver Nanoparticles Complex.
abstract: The aim of this work was to characterize the physico-chemical properties of 3-hydroxyflavone (3-HF) in a silver nanoparticles complex (SNPs) using UV-vis and Fluorescence spectroscopy, Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM) analysis. One also evaluated its effect on the cell viability and morphology of L929 mouse fibroblast cells in vitro. The contribution of the carrier protein, Bovine Serum Albumin (BSA) to 3-HF properties has also been investigated. 3-HF in BSA/SNPs systems presented no cytotoxic effect in L929 mouse fibroblast cells at any of the tested concentrations. The results are discussed with relevance to the oxidative stress process.
authors.affiliation: Institute of Physical Chemistry "Ilie Murgulescu" of the Romanian Academy, Splaiul Independentei 202, 060021, Bucharest, Romania. voicescu@icf.ro.
authors.forename: Mariana
authors.initials: M
authors.lastname: Voicescu
authors.affiliation: National Institute of R&D for Biological Sciences, Splaiul Independentei 296, 060031, Bucharest, Romania.
authors.forename: Oana
authors.initials: O
authors.lastname: Craciunescu
authors.affiliation: National Institute of R&D for Biological Sciences, Splaiul Independentei 296, 060031, Bucharest, Romania.
authors.forename: Lucia
authors.initials: L
authors.lastname: Moldovan
authors.affiliation: Institute of Physical Chemistry "Ilie Murgulescu" of the Romanian Academy, Splaiul Independentei 202, 060021, Bucharest, Romania.
authors.forename: Mihai
authors.initials: M
authors.lastname: Anastasescu
authors.affiliation: Institute of Physical Chemistry "Ilie Murgulescu" of the Romanian Academy, Splaiul Independentei 202, 060021, Bucharest, Romania.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Angelescu
authors.affiliation: Institute of Atomic Physics, National Institute of Materials Physics, Magurele, 077125, Romania.
authors.forename: Valentin S
authors.initials: VS
authors.lastname: Teodorescu
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D055598:Chemical Phenomena; D005419:Flavonoids / Q000737:chemistry* / Q000633:toxicity*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D012710:Serum Albumin, Bovine; D012834:Silver; C041477:3-hydroxyflavone
keywords: Cytotoxicity; Flavones; Fluorescence; Proteins; Silver nanoparticles