title: Regulation of vascular smooth muscle cell autophagy by DNA nanotube-conjugated mTOR siRNA.
abstract: The efficient delivery of short interfering RNA (siRNA) is an enormous challenge in the field of gene therapy. Herein, we report a delivery nanosystem based on programmed DNA self-assembly mammalian target of rapamycin (mTOR) siRNA-loaded DNA nanotubes (DNA-NTs). We demonstrate that these siRNA-DNA-NTs can be effectively transfected into pulmonary arterial smooth muscle cells (PASMCs) via endocytosis; and that the loaded mTOR siRNA can induce obvious autophagy and inhibit cell growth under both normal and hypoxic conditions. Moreover, we found that mTOR siRNA can control the autophagy and proliferation of PASMCs under hypoxic condition, suggesting a potential therapeutic application for mTOR siRNA in diseases involving abnormal autophagy in PASMCs.
authors.affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.
authors.forename: Zaichun
authors.initials: Z
authors.lastname: You
authors.affiliation: State Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Qian
authors.affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Changzheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Binfeng
authors.initials: B
authors.lastname: He
authors.affiliation: State Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. Electronic address: mao@purdue.edu.
authors.forename: Chengde
authors.initials: C
authors.lastname: Mao
authors.affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address: wanggs2003@hotmail.com.
authors.forename: Guansong
authors.initials: G
authors.lastname: Wang
mesh_terms: D001343:Autophagy / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010588:Phagosomes / Q000187:drug effects / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D014162:Transfection
chemical_list: D008869:Microtubule-Associated Proteins; D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D004247:DNA; D058570:TOR Serine-Threonine Kinases
keywords: Anoxia; Autophagy; DNA nanostructures; DNA nanotubes; Self-assembly; mTOR; siRNA