title: Anti-CD antibody microarray for human leukocyte morphology examination allows analyzing rare cell populations and suggesting preliminary diagnosis in leukemia.
abstract: We describe a method for leukocyte sorting by a microarray of anti-cluster-of-differentiation (anti-CD) antibodies and for preparation of the bound cells for morphological or cytochemical examination. The procedure results in a "sorted" smear with cells positive for certain surface antigens localised in predefined areas. The morphology and cytochemistry of the microarray-captured normal and neoplastic peripheral blood mononuclear cells are identical to the same characteristics in a smear. The microarray permits to determine the proportions of cells positive for the CD antigens on the microarray panel with high correlation with flow cytometry. Using the anti-CD microarray we show that normal granular lymphocytes and lymphocytes with radial segmentation of the nuclei are positive for CD3, CD8, CD16 or CD56 but not for CD4 or CD19. We also show that the described technique permits to obtain a pure leukemic cell population or to separate two leukemic cell populations on different antibody spots and to study their morphology or cytochemistry directly on the microarray. In cases of leukemias/lymphomas when circulating neoplastic cells are morphologically distinct, preliminary diagnosis can be suggested from full analysis of cell morphology, cytochemistry and their binding pattern on the microarray.
authors.affiliation: 1] Centre for Pediatric Hematology, Oncology and Immunology, Moscow, Russia [2] Centre for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Russia.
authors.forename: Alina N
authors.initials: AN
authors.lastname: Khvastunova
authors.affiliation: 1] Centre for Pediatric Hematology, Oncology and Immunology, Moscow, Russia [2] Centre for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Russia [3] Research Centre for Hematology, Moscow, Russia.
authors.forename: Sofya A
authors.initials: SA
authors.lastname: Kuznetsova
authors.affiliation: Research Centre for Hematology, Moscow, Russia.
authors.forename: Liubov S
authors.initials: LS
authors.lastname: Al-Radi
authors.affiliation: Research Centre for Hematology, Moscow, Russia.
authors.forename: Alexandra V
authors.initials: AV
authors.lastname: Vylegzhanina
authors.affiliation: 1] Centre for Pediatric Hematology, Oncology and Immunology, Moscow, Russia [2] Centre for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Russia.
authors.forename: Anna O
authors.initials: AO
authors.lastname: Zakirova
authors.affiliation: Centre for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Russia.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Fedyanina
authors.affiliation: Institute of Immunology, Moscow, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Filatov
authors.affiliation: A.N. Belozersky Institute and Department of Cell Biology and Histology, Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Ivan A
authors.initials: IA
authors.lastname: Vorobjev
authors.affiliation: 1] Centre for Pediatric Hematology, Oncology and Immunology, Moscow, Russia [2] Centre for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Russia [3] Research Centre for Hematology, Moscow, Russia.
authors.forename: Fazly
authors.initials: F
authors.lastname: Ataullakhanov
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D002469:Cell Separation; D042241:Early Diagnosis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007938:Leukemia / Q000276:immunology* / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D000912:Antibodies, Neoplasm; D015703:Antigens, CD