title: Draft genome sequence and characterization of Desulfitobacterium hafniense PCE-S.
abstract: This genome report describes the draft genome and the physiological characteristics of Desulfitobacterium hafniense PCE-S, a Gram-positive bacterium known to dechlorinate tetrachloroethene (PCE) to dichloroethene by a PCE reductive dehalogenase. The draft genome has a size of 5,666,696 bp with a G + C content of 47.3%. The genome is very similar to the already sequenced Desulfitobacterium hafniense Y51 and the type strain DCB-2. We identified two complete reductive dehalogenase (rdh) genes in the genome of D. hafniense PCE-S, one of which encodes PceA, the PCE reductive dehalogenase, and is located on a transposon. Interestingly, this transposon structure differs from the PceA-containing transposon of D. hafniense Y51. The second rdh encodes an unknown reductive dehalogenase, highly similar to rdhA 7 found in D. hafniense DCB-2, in which the corresponding gene is disrupted. This reductive dehalogenase might be responsible for the reductive dechlorination of 2,4,5-trichlorophenol and pentachlorophenol, which is mediated by D. hafniense PCE-S in addition to the reductive dechlorination of PCE.
authors.affiliation: Institut für Mikrobiologie, Lehrstuhl für Angewandte und Ökologische Mikrobiologie, University of Jena, Philosophenweg 12, Jena D-07743, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Goris
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen 6703 HB, The Netherlands ; Laboratory of Systems and Synthetic Biology, Wageningen University, Wageningen 6703 HB, The Netherlands.
authors.forename: Bastian
authors.initials: B
authors.lastname: Hornung
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen 6703 HB, The Netherlands.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kruse
authors.affiliation: Institut für Mikrobiologie, Lehrstuhl für Angewandte und Ökologische Mikrobiologie, University of Jena, Philosophenweg 12, Jena D-07743, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Reinhold
authors.affiliation: Electron Microscopy Center of the University Hospital Jena, Friedrich Schiller University, Jena, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Westermann
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University, Wageningen 6703 HB, The Netherlands.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schaap
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen 6703 HB, The Netherlands.
authors.forename: Hauke
authors.initials: H
authors.lastname: Smidt
authors.affiliation: Institut für Mikrobiologie, Lehrstuhl für Angewandte und Ökologische Mikrobiologie, University of Jena, Philosophenweg 12, Jena D-07743, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Diekert
keywords: Anaerobic respiration; Bioremediation; Chlorinated ethenes; Chlorinated phenols; Organohalide respiration; Reductive dechlorination; Reductive dehalogenase