title: Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification.
abstract: A new microsporidium, Nosema sp. PM-1, was first isolated from Papilio machaon Linnaeus. The spore shape of the PM-1 isolate was a long oval with an average size of 3.22 μm × 1.96 μm. Ultrastructure observation showed that PM-1 had a typical Nosema common diplokaryotic nuclei structure with 10-13 polar filament coils, spore wall, plasma membrane, and anchoring disk. The complete rRNA gene sequences were obtained by polymerase chain reaction amplification and each rRNA unit was arrayed as follows: 5'-LSU (2497 bp)-ITS (179 bp)-SSU (1232 bp)-IGS (278 bp)-5S (115 bp)-3', which was the same as typical Nosema. The phylogenetic trees of rRNA, DNA-directed RNA polymerase II subunit, and tubulin genes all show that PM-1 was a sister to the clade comprising Nosema bombycis, Nosema spodopterae, and Nosema sp. PX1. The spore morphology, ultrastructure, and complete rRNA structure indicate that this isolate assigned to the ˝true˝ Nosema group, can parasitized in Papilio machaon Linnaeus, which provides a wider host range for Nosema.
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.forename: Jinshan
authors.initials: J
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Zeng
authors.initials: Z
authors.lastname: Li
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Guoqing
authors.initials: G
authors.lastname: Pan
authors.forename: Xueyan
authors.initials: X
authors.lastname: Li
authors.forename: Zeyang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002080:Butterflies / Q000382:microbiology*; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016818:Nosema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000302:isolation & purification / Q000648:ultrastructure; D014404:Tubulin / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065096:RNA, ribosomal, 26S; D014404:Tubulin; D012319:RNA Polymerase II