title: Advanced Crystallographic Data Collection Protocols for Experimental Phasing.
abstract: Experimental phasing by single- or multi-wavelength anomalous dispersion (SAD or MAD) has become the most popular method of de novo macromolecular structure determination. Continuous advances at third-generation synchrotron sources have enabled the deployment of rapid data collection protocols that are capable of recording SAD or MAD data sets. However, procedural simplifications driven by the pursuit of high throughput have led to a loss of sophistication in data collection strategies, adversely affecting measurement accuracy from the viewpoint of anomalous phasing. In this chapter, we detail optimized strategies for collecting high-quality data for experimental phasing, with particular emphasis on minimizing errors from radiation damage as well as from the instrument. This chapter also emphasizes data processing for "on-the-fly" decision-making during data collection, a critical process when data quality depends directly on information gathered while at the synchrotron.
authors.affiliation: Paul Scherrer Institute, Swiss Light Source, Villigen, 5232, Switzerland.
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Finke
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Panepucci
authors.forename: Clemens
authors.initials: C
authors.lastname: Vonrhein
authors.forename: Meitian
authors.initials: M
authors.lastname: Wang
authors.forename: Gérard
authors.initials: G
authors.lastname: Bricogne
authors.forename: Vincent
authors.initials: V
authors.lastname: Oliéric
mesh_terms: D000465:Algorithms; D003196:Computer Graphics; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D003625:Data Collection; D003657:Decision Making; D004867:Equipment Design; D046911:Macromolecular Substances; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012984:Software; D013050:Spectrometry, Fluorescence; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D046911:Macromolecular Substances