title: Polymer-Coated Hollow Mesoporous Silica Nanoparticles for Triple-Responsive Drug Delivery.
abstract: In this study, pH, reduction and light triple-responsive nanocarriers based on hollow mesoporous silica nanoparticles (HMSNs) modified with poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) were developed via surface-initiated atom transfer radical polymerization. Both reduction-cleavable disulfide bond and light-cleavable o-nitrobenzyl ester were used as the linkages between HMSNs and pH-sensitive PDEAEMA polymer caps. A series of characterization techniques were applied to characterize and confirm the structures of the intermediates and final nanocarriers. Doxorubicin (DOX) was easily encapsulated into the nanocarriers with a high loading capacity, and quickly released in response to the stimuli of reducing agent, acid environment or UV light irradiation. In addition, flow cytometry analysis, confocal laser scanning microscopy observations and cytotoxicity studies indicated that the nanocarriers were efficiently internalized by HeLa cancer cells, exhibiting (i) enhanced release of DOX into the cytoplasm under external UV light irradiation, (ii) better cytotoxicity against HeLa cells, and (iii) superior control over drug delivery and release. Thus, the triple-responsive nanocarriers present highly promising potentials as a drug delivery platform for cancer therapy.
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.
authors.forename: Chung Yen
authors.initials: CY
authors.lastname: Ang
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.
authors.forename: Menghuan
authors.initials: M
authors.lastname: Li
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.
authors.forename: Si Yu
authors.initials: SY
authors.lastname: Tan
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Qu
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Luo
authors.affiliation: †Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.|‡School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004220:Disulfides / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry / Q000633:toxicity; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D065546:Drug Liberation / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009757:Nylons / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014466:Ultraviolet Rays
chemical_list: D000903:Antibiotics, Antineoplastic; D004220:Disulfides; D004337:Drug Carriers; D008689:Methacrylates; D009757:Nylons; C109846:poly(2-(diethylamino)ethyl methacrylate); D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: controlled drug delivery; hollow mesoporous silica nanoparticles; nanocarriers; polymer coating; triple-responsive release