title: 2D map projections for visualization and quantitative analysis of 3D fluorescence micrographs.
abstract: We introduce Map3-2D, a freely available software to accurately project up to five-dimensional (5D) fluorescence microscopy image data onto full-content 2D maps. Similar to the Earth's projection onto cartographic maps, Map3-2D unfolds surface information from a stack of images onto a single, structurally connected map. We demonstrate its applicability for visualization and quantitative analyses of spherical and uneven surfaces in fixed and dynamic live samples by using mammalian and yeast cells, and giant unilamellar vesicles. Map3-2D software is available at http://www.zmbh.uni-heidelberg.de//Central_Services/Imaging_Facility/Map3-2D.html.
authors.affiliation: Center of Molecular Biology, University of Heidelberg (ZMBH), Heidelberg, Germany.
authors.forename: G Hernán
authors.initials: GH
authors.lastname: Sendra
authors.affiliation: Center of Molecular Biology, University of Heidelberg (ZMBH), Heidelberg, Germany.
authors.forename: Christian H
authors.initials: CH
authors.lastname: Hoerth
authors.affiliation: 1] Institut Curie -Centre de Recherche, Endocytic Trafficking and Therapeutic Delivery group, Paris, France [2] CNRS UMR3666, INSERM U1143, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Wunder
authors.affiliation: Center of Molecular Biology, University of Heidelberg (ZMBH), Heidelberg, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Lorenz