title: Cultivation of Chlorella sp. GD using piggery wastewater for biomass and lipid production.
abstract: The development of a culture system for Chlorella sp. GD to efficiently produce biomass and oil for biodiesel production was investigated. Chlorella sp. GD was cultivated with 0%, 25%, 50%, 75% and 100% piggery wastewater (diluted by medium) at 300 μmol m(-2) s(-1), a 2% CO2 aeration rate of 0.2 vvm and 26±1°C; after a 10-day culture in batch cultures, the maximum specific growth rate and biomass productivity of the microalga obtained in 100% piggery wastewater were 0.839 d(-1) and 0.681 g L(-1) d(-1), respectively. The highest lipid content and lipid productivity were 29.3% and 0.155 g L(-1) d(-1) at 25% wastewater, respectively. In semi-continuous cultures, the biomass and lipid productivities with 25-75% wastewater ratios were greater than 0.852 and 0.128 g L(-1) d(-1), respectively. These results show that Chlorella sp. GD grows efficiently in piggery wastewater, and that a stable growth performance was achieved for long-term microalgal cultivation in a semi-continuous culture.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Chiu-Mei
authors.initials: CM
authors.lastname: Kuo
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Tsai-Yu
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Lin
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; Aquatic Technology Laboratories, Agricultural Technology Research Institute, Hsinchu, Taiwan.
authors.forename: Chien-Ya
authors.initials: CY
authors.lastname: Kao
authors.affiliation: Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.
authors.forename: Jinn-Tsyy
authors.initials: JT
authors.lastname: Lai
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan. Electronic address: lincs@mail.nctu.edu.tw.
authors.forename: Chih-Sheng
authors.initials: CS
authors.lastname: Lin
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000254:growth & development*; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development*; D013552:Swine; D062065:Wastewater
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D062065:Wastewater
keywords: Biomass; Lipid; Microalgae; Semi-continuous culture; Wastewater