title: Human norovirus genogroup II recombinants in Thailand, 2009-2014.
abstract: Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis worldwide. New strains emerge partly due to viral recombination. In Thailand, there is a lack of data on NoV recombinants among clinical isolates. We screened stool samples from pediatric diarrheal patients for norovirus by RT-PCR and found GII.4 to be the most prevalent genotype. Phylogenetic and SimPlot analyses detected seven intra-genogroup recombinant strains: three GII.21/GII.3, two GII.12/GII.3, and two GII.12/GII.1 recombinants. Maximum chi-square analysis indicated that all had similar breakpoints near the ORF1/ORF2 junction (p < 0.001), either slightly upstream within the C-terminus of RdRp or downstream within the N-terminal domain of VP1.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Tikumporn
authors.initials: T
authors.lastname: Phumpholsup
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand.|Division of Molecular Medicine, Department of Research and Development, Faculty of Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Chieochansin
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Sompong
authors.initials: S
authors.lastname: Vongpunsawad
authors.affiliation: Chum Phae Hospital, Chum Phae, Khon Kaen, Thailand.
authors.forename: Viboonsuk
authors.initials: V
authors.lastname: Vuthitanachot
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand. yong.p@chula.ac.th.
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D017250:Caliciviridae Infections / Q000821:virology*; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D013785:Thailand
chemical_list: D012367:RNA, Viral
keywords: Genome recombination; Norovirus; Prevalence; Thailand