title: Brief Exercises Affect Gene Expression in Circulating Monocytes.
abstract: We aimed to give a systematic hypothesis on the functions of exercise on circulating monocytes by identifying a discrete set of genes in circulating monocytes that were altered by exercise. The microarray expression profile of GSE51835 was downloaded from gene expression omnibus (GEO) database for the identification of differentially expressed genes (DEGs) using limma and affy packages in R language. Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed for DEGs, followed by the construction of co-expression network and protein-protein interaction (PPI) network. The top 10 nodes in PPI network were screened, and subnetwork was constructed for the key genes identification. Totally, 35 DEGs, including 2 upregulated genes and 33 downregulated genes, were identified. The enriched GO terms were mainly linked to immune response and defence response, and the enriched KEGG pathways were mainly associated with natural killer cell-mediated cytotoxicity and graft-versus-host disease. Dual-specificity phosphatase 2 (DUSP2) was identified as a key node in the co-expression network. In the PPI network, CD247 module (CD247), chemokine (C-X-C motif) receptor 4 (CXCR4), granzyme B (GZMB) and perforin 1 (PRF1) were identified as key nodes. An important interaction, GZMB/PRF1, was detected. Five key genes, including DUSP2, CD247, CXCR4, GZMB and PRF1, and an interaction of GZMB/PRF1, were significant factors in the immune processes of circulating monocytes, which might be regulated by brief exercises, leading to the enhancement of immune function.
authors.affiliation: Emergency Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.affiliation: Rehabilitation Medicine Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: F
authors.initials: F
authors.lastname: Cai
authors.affiliation: Digestive Department, Jilin Province People'S Hospital, Changchun, Jilin Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Ge
authors.affiliation: Rehabilitation Medicine Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: L
authors.initials: L
authors.lastname: Yin
mesh_terms: D000328:Adult; D001775:Blood Circulation / Q000235:genetics; D017252:CD3 Complex / Q000235:genetics; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D054641:Dual Specificity Phosphatase 2 / Q000235:genetics; D015444:Exercise / Q000502:physiology; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D053804:Granzymes / Q000235:genetics; D006801:Humans; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D046228:Microarray Analysis; D009000:Monocytes / Q000502:physiology*; D054353:Perforin / Q000235:genetics; D060066:Protein Interaction Maps; D019718:Receptors, CXCR4 / Q000235:genetics; D055815:Young Adult
chemical_list: D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; C527276:CXCR4 protein, human; C510468:PRF1 protein, human; D019718:Receptors, CXCR4; D054353:Perforin; C517032:DUSP2 protein, human; D054641:Dual Specificity Phosphatase 2; D053804:Granzymes