title: A microfluidic device for epigenomic profiling using 100 cells.
abstract: The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.
authors.affiliation: Department of Biomedical Engineering and Mechanics, Virginia Tech, Blacksburg, Virginia, USA.
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Changya
authors.initials: C
authors.lastname: Chen
authors.affiliation: Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: He
authors.affiliation: Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, Iowa, USA.|Department of Internal Medicine, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Tan
authors.affiliation: Department of Chemical Engineering, Virginia Tech, Blacksburg, Virginia, USA.
authors.forename: Chang
authors.initials: C
authors.lastname: Lu
mesh_terms: D000107:Acetylation; D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000295:instrumentation* / Q000379:methods*; D004742:Enhancer Elements, Genetic; D057890:Epigenomics / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006657:Histones / Q000378:metabolism; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008099:Liver / Q000196:embryology / Q000502:physiology; D008297:Male; D066192:Manufacturing Industry / Q000379:methods; D008810:Mice, Inbred C57BL
chemical_list: D006657:Histones