title: [¹¹C]acetate and PET/CT assessment of muscle activation in rat studies.
abstract: The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [[Formula: see text]]acetate positron emission tomography and computed tomography (PET/CT).
  
  
  
  Contractions were induced in Wistar rats' left hindlimb by electrostimulation of the Vastus Lateralis muscle motor point. After 15 min of muscle contractions, [[Formula: see text]]acetate was injected and PET/CT of both hindlimbs was acquired. The resting hindlimb was used as a control reference. The kinetic parameters [Formula: see text] and [Formula: see text] were calculated for the target muscles (exercised and control) and correlated with the corresponding standardized uptake values (SUVs). The ratio between each kinetic parameter values and the SUV extracted for the exercised muscle and the muscle at rest was computed ([Formula: see text] and [Formula: see text], respectively).
  
  
  
  Kinetic analysis quantitatively confirmed that net tracer uptake ([Formula: see text]) and washout ([Formula: see text]) were significantly higher in exercised muscles ([Formula: see text] for exercised muscles vs. [Formula: see text] for resting muscles, [Formula: see text]; [Formula: see text] for exercised muscle vs. [Formula: see text] for resting muscle, [Formula: see text]). On the other hand, SUV was not significantly different between active and inactive muscles ([Formula: see text] for exercised muscles vs. [Formula: see text] for resting muscles). Linear regression analysis revealed a good correlation ([Formula: see text]) between net tracer uptake ratio ([Formula: see text]) and the SUV ratio [Formula: see text]). A lower correlation was found between the net tracer washout ratio ([Formula: see text]) and the SUV ratio ([Formula: see text]).
  
  
  
  The present study showed that kinetic modelling can detect changes between active and inactive skeletal muscles with a higher sensitivity with respect to the SUV, when performed with [[Formula: see text]]acetate PET/CT.
authors.affiliation: University of Geneva and University Hospitals of Geneva, 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland. Sara.Trombella@hcuge.ch.
authors.forename: Sara
authors.identifier: 0000-0001-6299-2066
authors.initials: S
authors.lastname: Trombella
authors.affiliation: University of Geneva and University Hospitals of Geneva, 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: García
authors.affiliation: University Hospitals of Geneva and Centre for Biomedical Imaging (CIBM), 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
authors.forename: Didier J
authors.initials: DJ
authors.lastname: Colin
authors.affiliation: University Hospitals of Geneva and Centre for Biomedical Imaging (CIBM), 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Germain
authors.affiliation: University Hospitals of Geneva, 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
authors.forename: Yann
authors.initials: Y
authors.lastname: Seimbille
authors.affiliation: University Hospitals of Geneva, 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
authors.forename: Osman
authors.initials: O
authors.lastname: Ratib
mesh_terms: D000085:Acetates; D000818:Animals; D002250:Carbon Radioisotopes; D006614:Hindlimb / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000378:metabolism; D000072078:Positron Emission Tomography Computed Tomography; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes
keywords: Kinetic analysis; Muscle contraction; PET/CT; SUV; []acetate