title: Isolation and characterization of fish scale collagen from tilapia (Oreochromis sp.) by a novel extrusion-hydro-extraction process.
abstract: Collagen is highly valued both as a food additive and a functional food ingredient. It is generally extracted by treatments with acid or alkali, enzyme, and microorganisms. However these methods are generally batch type, time-, energy-, reactant-, and cost-consuming. Extrusion is widely used in the food industry, and offers many advantages, such as ease of operation, continuous production, high yield, and little waste. In this study, we developed a novel extrusion-hydro-extraction (EHE) process for extraction of collagen from tilapia fish scale. Extruded scale samples had a 2-3 times higher protein extraction yield than that of non-extruded scale samples. All extracts contained hydroxyproline (61-73 residues/1000 residues) and hydroxylysine (5-6 residues/1000 residues) and were identified as type-I collagens by FTIR, SDS-PAGE, and molecular weight distribution analyses. The physicochemical studies revealed that extracted collagens could have promising applications in the food, medical, and cosmetic industries.
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung 811, Taiwan, ROC. Electronic address: cyhuang@webmail.nkmu.edu.tw.
authors.forename: Chun-Yung
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung 811, Taiwan, ROC.
authors.forename: Jen-Min
authors.initials: JM
authors.lastname: Kuo
authors.affiliation: Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, No. 60, Sec. 1, Erh-Jen Rd., Jen-Te District, Tainan 717, Taiwan, ROC.
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Wu
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung 811, Taiwan, ROC.
authors.forename: Hsing-Tsung
authors.initials: HT
authors.lastname: Tsai
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000032:analysis / Q000302:isolation & purification*; D006909:Hydroxyproline / Q000032:analysis; D017210:Tilapia / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D006909:Hydroxyproline
keywords: Collagen; Extrudate; Extrusion; Hot water extraction; Tilapia fish scale