title: Effect of VTCN1 on progression and metastasis of ovarian carcinoma in vitro and vivo.
abstract: OBJECTIVE
  Through reducing immune response, VTCN1 could promote carcinoma indirectly. However, the direct effect of VTCN1 on carcinoma was not studied clearly, especially on ovarian carcinoma. In this paper, we verified the potential effect and mechanism of VTCN1 on ovarian carcinoma.
  
  
  METHODS
  The influence of high or low VTCN1 expression on the viability of ovarian cancer was detected by CKK-8 and annexin V-PI kit. The orthotopicxenograft tumor model was performed to evaluate the effect of VTCN1 on the promotion of tumor in vivo. Western blot was used to verify the signaling pathways predicted by bioinformatics analysis.
  
  
  RESULTS
  Low expression of VTCN1 could inhibit the viability and metastasis of ovarian carcinoma directly in vitro and vivo; Information analysis demonstrated that cell cycle and JAK2/STAT were involved in the regulation of VTCN1. The CDK2/4 and CDC25C expression and phosphorylation of JAK2/STAT had a direct relationship with the reduction of VTCN1.
  
  
  CONCLUSIONS
  VTCN1 could affect the viability and metastasis of ovarian carcinoma by reducing the expression of CDK2/4 and CDC25C and phosphorylation of JAK2/STAT. It indicated that VTCN1 was a potential target for treating ovarian carcinoma.
authors.affiliation: Department of obstetrics and gynecology, the 2nd affiliated hospital of Soochow University, Soochow, People's Republic of China. Electronic address: 13906135277@163.com.
authors.forename: Aihua
authors.initials: A
authors.lastname: Gao
authors.affiliation: College of pharmacy, Suzhou University, Suzhou, People's Republic of China. Electronic address: liangzhang323456@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of obstetrics and gynecology, the 2nd affiliated hospital of Soochow University, Soochow, People's Republic of China. Electronic address: xinchen323456@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of obstetrics and gynecology, the 2nd affiliated hospital of Soochow University, Soochow, People's Republic of China. Electronic address: yingchen323456@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of obstetrics and gynecology, the 2nd affiliated hospital of Soochow University, Soochow, People's Republic of China. Electronic address: zhenzhenxu323456@163.com.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of obstetrics and gynecology, the 2nd affiliated hospital of Soochow University, Soochow, People's Republic of China. Electronic address: yananliu323456@163.com.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of obstetrics and gynecology, the 2nd affiliated hospital of Soochow University, Soochow, People's Republic of China. Electronic address: yin323456@163.com.
authors.forename: Weipei
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1 / Q000096:biosynthesis*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1; C475826:VTCN1 protein, human
keywords: Information analysis; Ovarian carcinoma; VTCN1