title: Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability.
abstract: Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2β antibody. Knockdown of the XLAP2β protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis.
authors.affiliation: Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wrocław, Joliot- Curie 14a, 50-383, Wrocław, Poland.|Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21, 50-335, Wroclaw, Poland.
authors.forename: Magda
authors.initials: M
authors.lastname: Dubińska-Magiera
authors.affiliation: Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wrocław, Joliot- Curie 14a, 50-383, Wrocław, Poland.|Department of Evolutionary Biology and Vertebrate Conservation, University of Wroclaw, Sienkiewicza 21, 50-335, Wroclaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmielewska
authors.affiliation: Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wrocław, Joliot- Curie 14a, 50-383, Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kozioł
authors.affiliation: Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wrocław, Joliot- Curie 14a, 50-383, Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Machowska
authors.affiliation: Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham, DH1 3LE, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hutchison
authors.affiliation: Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham, DH1 3LE, UK.
authors.forename: Martin W
authors.initials: MW
authors.lastname: Goldberg
authors.affiliation: Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wrocław, Joliot- Curie 14a, 50-383, Wrocław, Poland. rzepecki@ibmb.uni.wroc.pl.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Rzepecki
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D002470:Cell Survival / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D007399:Interphase / Q000235:genetics; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008938:Mitosis; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D034921:Lamin Type B; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; C486551:TPX2 protein, Xenopus; D029867:Xenopus Proteins; C093413:lamina-associated polypeptide 2
keywords: Cell nucleus; Knockdown; LAP2; Lamin B; Nucleoporin; TPX2; Xenopus laevis