title: Nanoscopic leg irons: harvesting of polymer-stabilized membrane proteins with antibody-functionalized silica nanoparticles.
abstract: Silica-based nanoparticles (SiNPs) are presented to harvest complex membrane proteins, which have been embedded into unilammelar polymersomes via in vitro membrane assisted protein synthesis (iMAP). Size-optimized SiNPs have been surface-modified with polymer-targeting antibodies, which are employed to harvest the protein-containing polymersomes. The polymersomes mimic the cellular membrane. They are chemically defined and preserve their structural-functional integrity as virtually any membrane protein species can be synthesized into such architecture via the ribosomal context of a cellular lysate. The SiNPs resemble 'heavy leg irons' catching the polymersomes in order to enable gravity-based generic purification and concentration of such proteopolymersomes from the crude mixture of cellular lysates.
authors.affiliation: Department for Nanobiotechnology, Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Science, Muthgasse 11/2, 1190 Vienna, Austria. eva.sinner@boku.ac.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Zapf
authors.forename: Christian
authors.initials: C
authors.lastname: Zafiu
authors.forename: Christoph
authors.initials: C
authors.lastname: Zaba
authors.forename: Cherng-Wen Darren
authors.initials: CW
authors.lastname: Tan
authors.forename: Walter
authors.initials: W
authors.lastname: Hunziker
authors.forename: Eva-Kathrin
authors.initials: EK
authors.lastname: Sinner
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000906:Antibodies; D008565:Membrane Proteins; D011108:Polymers; D012822:Silicon Dioxide