title: Phenolic Compounds from the Roots of Rhodiola crenulata and Their Antioxidant and Inducing IFN-γ Production Activities.
abstract: In the present study, two new phenolic compounds 1 and 11, a pair of lignan isomers 12 and 13 with their absolute configurations established for the first time, were isolated from the ethanol extract of the roots of Rhodiola crenulata, together with 13 known phenolic compounds, and their structures were elucidated via NMR, HRESIMS, UV, IR and CD analyses. All the isolated compounds were evaluated for their in vitro antioxidant activities using the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. Ten of them exhibited significant antioxidant activities compared to ascorbic acid. Furthermore, the inducibilities of the isolated compounds to IFN-γ production were also assessed. Compounds 1, 8, 9, 12, 13, 14 and 15 could moderately stimulate IFN-γ expression.
authors.affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. zjt19881206@126.com.|Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China. zjt19881206@126.com.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, School of Medicine, Shenzhen University, Shenzhen 518060, China. lichenyangchina@163.com.
authors.forename: Chen-Yang
authors.initials: CY
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. pharmwch@126.com.|Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China. pharmwch@126.com.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. 13516185421@139.com.
authors.forename: Yue-Fei
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, School of Medicine, Shenzhen University, Shenzhen 518060, China. wangxiaodong@szu.edu.cn.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wang
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China. wht72007@126.com.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. yanzhu.harvard@gmail.com.|Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China. yanzhu.harvard@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. miaomiaojiang@126.com.|Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China. miaomiaojiang@126.com.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Jiang
authors.affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. gaoxiumei@tjutcm.edu.cn.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Gao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007369:Interferon Inducers / Q000737:chemistry / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*
chemical_list: D016166:Free Radical Scavengers; D007369:Interferon Inducers; D010936:Plant Extracts; D000431:Ethanol; D007371:Interferon-gamma
keywords: IFN-γ production; Rhodiola crenulata; antioxidant activity; immunomodulatory effect; phenol