title: ISOLATION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE CRUDE POLYSACCHARIDE FROM PHYLLOPHORA PSEUDOCERANOIDES.
abstract: The red seaweed Phyllophora pseudoceranoides (Gmelin) New. et Tayl, commonly found in the Romanian Black Sea coastal waters, has not been studied regarding its chemical composition and biological activities.
  
  
  OBJECTIVE
  The aim of the present study was to isolate, characterize and assess the in vitro antioxidant activity of the crude polysaccharide from P. pseudocerano ides.
  
  
  METHODS
  The water soluble polysaccharide was characterized by Fourier transform infrared spectroscopy (FT-IR). Total phenolic content was determined by Folin-Ciocalteu method. Antioxidant activity was evaluated by ABTS radical cation scavenging and reducing power assays. Commercial-grade carrageenan was used as positive control.
  
  
  CONCLUSIONS
  FT-IR analysis of the crude polysaccharide showed characteristic bands of carrageenan-type structure. A total phenolic content of 25.08 ± 1.00 mg GAE/g was determined in the crude polysaccharide. At 1.5 mg/mL, the polysaccharide exhibited important ABTS scavenging activity (49.59 ± 0.03%) and showed a good reducing power (0.4060 ± 0.002), when compared with the positive control (ABTS scavenging activity: 30.30 ± 0.03% and reducing power: 0.2494 ± 0.002, at a concentration of 1.5 mg/mL).
  
  
  CONCLUSIONS
  The antioxidant activity of the sulfated polysaccharide suggests its possible use as an ingredient and antioxidant agent in the food and pharmaceutical industries.
authors.forename: Adriana
authors.initials: A
authors.lastname: Trifan
authors.forename: Daciana
authors.initials: D
authors.lastname: Sava
authors.forename: Laura
authors.initials: L
authors.lastname: Bucur
authors.forename: Al
authors.initials: A
authors.lastname: Vasincu
authors.forename: Ioana
authors.initials: I
authors.lastname: Vasincu
authors.forename: Ana Clara
authors.initials: AC
authors.lastname: Aprotosoaie
authors.forename: Oana
authors.initials: O
authors.lastname: Cioancă
authors.forename: Anca
authors.initials: A
authors.lastname: Miron
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D059038:Black Sea; D002351:Carrageenan / Q000737:chemistry; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D000461:Rhodophyta; D012383:Romania; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D011134:Polysaccharides; D002351:Carrageenan