title: [Expression of Ki-67 and estrogen receptor in the uterus of mice with autoimmune premature ovarian failure induced by peptide zona pellucida 3].
abstract: OBJECTIVE
  To investigate the histomorphology and the expressions of the proliferation marker Ki-67 and estrogen receptor in the uterus of mice with autoimmune premature ovarian failure (POF) induced by zona pellucida 3 peptide (pZP3).
  
  
  METHODS
  Autoimmune POP models were established in 20 female BALB/c mice (7-8 weeks old) by immunization with pZP3 and another 20 mice served as the control group. The POP models were verified by vaginal cytology, serum sex hormones, ovary histomorphology and ZP3 antibody immunohistochemistry. The histomorphology and expressions of Ki-67, estrogen receptor α and estrogen receptor β in the uterus of the mice were detected.
  
  
  RESULTS
  Autoimmune POP models were established successfully in 80% of the mice at 8 weeks after the immunization. Compared with those in the control group, the mice in the model group showed a smaller volume of the uterus, thinner endometrium and a reduced number of glands. The luminal epithelial cells, glandular epithelial cells and stromal cells in the uterus of the model mice all presented with a lower expression of Ki-67 than those in the control group, and Ki-67 translocation from the nuclei to the cytoplasm was found in the model group. The luminal epithelial cells, glandular epithelial cells and stromal cells showed positive ERα immunoreactivity in the model group but not in the control group. No obvious ERβ expression was found in the uterus in either of the groups.
  
  
  CONCLUSIONS
  pZP3 can induce autoimmune POP, cause suppressed proliferation of the endometrial epithelial cells and stromal cells, and reduce the cellular expression of ERα in the uterus of mice.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.E-mail: chh2004@126.com.
authors.forename: Huihua
authors.initials: H
authors.lastname: Cai
authors.forename: Xiafei
authors.initials: X
authors.lastname: Fu
authors.forename: Xuwen
authors.initials: X
authors.lastname: Ren
authors.forename: Xiazhu
authors.initials: X
authors.lastname: Chen
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Yuanli
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism; D002467:Cell Nucleus; D004527:Egg Proteins; D004717:Endometrium; D004847:Epithelial Cells; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D016649:Primary Ovarian Insufficiency / Q000378:metabolism*; D011956:Receptors, Cell Surface; D017154:Stromal Cells; D014599:Uterus / Q000378:metabolism*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse