title: Epiphytic marine pigmented bacteria: A prospective source of natural antioxidants.
abstract: Awareness on antioxidants and its significance in human healthcare has increased many folds in recent time. Increased demand requisite on welcoming newer and alternative resources for natural antioxidants. Seaweed associated pigmented bacteria screened for its antioxidant potentials reveals 55.5% of the organisms were able to synthesize antioxidant compounds. DPPH assay showed 20% of the organisms to reach a antioxidant zone of 1 cm and 8.3% of the strains more than 3 cm. Pseudomonas koreensis (JX915782) a Sargassum associated yellowish brown pigmented bacteria have better activity than known commercial antioxidant butylated hydroxytoluene (BHT) against DPPH scavenging. Serratia rubidaea (JX915783), an associate of Ulva sp. and Pseudomonas argentinensis (JX915781) an epiphyte of Chaetomorpha media , were also contributed significantly towards ABTS (7.2% ± 0.03 to 15.2 ± 0.09%; 1.8% ± 0.01 to 15.7 ± 0.22%) and FRAP (1.81 ± 0.01 to 9.35 ± 0.98; 7.97 ± 0.12 to 18.70 ± 1.84 μg/mL of AsA Eq.) respectively. 16S rRNA gene sequence analysis revealed bacteria that have higher antioxidant activity belongs to a bacterial class Gammaproteobacteria. Statistical analysis of phenolic contents in relation with other parameters like DPPH, ABTS, reducing power and FRAP are well correlated (p < 0.05). Results obtained from the current study inferred that the seaweed associated pigmented bacteria have enormous potential on antioxidant compounds and need to be extracted in a larger way for clinical applications.
authors.affiliation: Biological Oceanography Division, National Institute of Oceanography, Panaji, India.
authors.forename: Ravindra
authors.initials: R
authors.lastname: Pawar
authors.affiliation: Biological Oceanography Division, National Institute of Oceanography, Panaji, India.
authors.forename: Chellandi
authors.initials: C
authors.lastname: Mohandass
authors.affiliation: Nallamuthu Gounder Mahalingam Centre for Research and Development, Nallamuthu Gounder Mahalingam College, Bharathiar University, Tamilnadu, India.
authors.forename: Elakkiya
authors.initials: E
authors.lastname: Sivaperumal
authors.affiliation: Biological Oceanography Division, National Institute of Oceanography, Panaji, India.
authors.forename: Elaine
authors.initials: E
authors.lastname: Sabu
authors.affiliation: Biological Oceanography Division, National Institute of Oceanography, Panaji, India.
authors.forename: Raju
authors.initials: R
authors.lastname: Rajasabapathy
authors.affiliation: Cholamandalam MS Risk Services Pvt Ltd., Panaji, India.
authors.forename: Tanaji
authors.initials: T
authors.lastname: Jagtap
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D059001:Aquatic Organisms / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010860:Pigments, Biological / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012624:Seaweed / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D000975:Antioxidants; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S
keywords: antioxidant; epiphytic; pigmented bacteria; seaweeds