title: Identification, inheritance, and fitness costs of Cry2Ab2 resistance in a field-derived population of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae).
abstract: The sugarcane borer, Diatraea saccharalis (F.), is one of the major target pests of transgenic maize, Zea mays, expressing Bacillus thuringiensis (Bt) proteins in South America and mid-southern region of the U.S. The MON89034 maize expresses Cry2Ab2 and Cry1A.105 Bt proteins and it was developed to provide better control of key lepidopteran pests of maize including D. saccharalis. The objectives of this study were to select and characterize the resistance of D. saccharalis to Cry2Ab2 using a non-commercial Cry2Ab2 single gene Bt maize line. A Cry2Ab2-resistant strain (Cry2Ab2-RR) of D. saccharalis was established from 28 two-parent families collected from fields in northeast Louisiana, U.S. The Cry2Ab2-RR showed a high level of resistance to Cry2Ab2 in both diet-incorporated and whole maize plant bioassays. The Cry2Ab2 resistance in D. saccharalis was likely inherited as a single or a few tightly linked autosomal genes. The resistance was non-recessive and not associated with fitness costs. The results should provide valuable information in resistance monitoring, assessing resistance risk, and developing effective management strategies for the sustainable use of Bt maize technology for managing maize stalk borers.
authors.affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA. Electronic address: fhuang@agcenter.lsu.edu.
authors.forename: Fangneng
authors.initials: F
authors.lastname: Huang
authors.affiliation: Monsanto Company, Chesterfield, MO 63017, USA.
authors.forename: Mao
authors.initials: M
authors.lastname: Chen
authors.affiliation: Monsanto Company, Chesterfield, MO 63017, USA.
authors.forename: Anilkumar
authors.initials: A
authors.lastname: Gowda
authors.affiliation: Monsanto Company, Chesterfield, MO 63017, USA.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Clark
authors.affiliation: Monsanto Company, Chesterfield, MO 63017, USA.
authors.forename: Brain C
authors.initials: BC
authors.lastname: McNulty
authors.affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Niu
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics*; D009036:Moths / Q000235:genetics*; D010572:Pest Control, Biological / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D003313:Zea mays / Q000235:genetics / Q000469:parasitology*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Cry2Ab; Diatraea saccharalis; Fitness costs; Inheritance; Resistance