title: Biological activity of the essential oils from Cinnamodendron dinisii and Siparuna guianensis.
abstract: This study had analyzed the antibacterial, antifungal and trypanocidal activity of the essential oils from Cinnamodendron dinisii Schwacke (Canellaceae) and Siparuna guianensis Aublet (Siparunaceae). The essential oils were obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. Chemical analysis by gas-liquid chromatography coupled to mass spectrometry (GC-MS) showed that these essential oils are rich in monoterpene and sesquiterpene hydrocarbons. Activity against the pathogenic bacteria Escherichia coli , Listeria monocytogenes , Pseudomonas aeruginosa , Salmonella choleraesuis and Staphylococcus aureus was evaluated with the agar cavity diffusion method, while activity on the filamentous fungi Aspergillus flavus , Aspergillus niger , Aspergillus carbonarius and Penicillium commune was evaluated by the disk diffusion technique. Trypanocidal activity was tested against Trypanosoma cruzi epimastigotes, using the Tetrazolium salt (MTT) colorimetric assay. Both essential oils exhibited low inhibitory effect towards bacteria, showing high MIC values (125-500 μg mL (-1) ), with Gram positive bacteria being more susceptible. Better inhibitory effect was obtained for the evaluated fungi, with lower MIC values (7.81-250 μg mL (-1) ), being A. flavus the most susceptible species. Both essential oils presented low trypanocidal activity, with IC 50 /24 h values of 209.30 μg mL (-1) for S. guianensis and 282.93 μg mL (-1) for C. dinisii . Thus, the high values observed for the MIC of evaluated bacteria and for IC 50 /24 h of T. cruzi , suggest that the essential oils have a low inhibitory activity against these microorganisms. In addition, the low MIC values observed for the tested fungi species indicate good inhibitory activity on these microorganisms's growth.
authors.affiliation: Departamento de Química, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Milene Aparecida
authors.initials: MA
authors.lastname: Andrade
authors.affiliation: Departamento de Química, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Maria das Graças
authors.initials: Md
authors.lastname: Cardoso
authors.affiliation: Departamento de Química, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Marcos de Souza
authors.initials: Mde S
authors.lastname: Gomes
authors.affiliation: Laboratório de Biologia Celular, Instituto Carlos Chagas, Curitiba, PR, Brazil.
authors.forename: Camila Maria Oliveira
authors.initials: CM
authors.lastname: de Azeredo
authors.affiliation: Departamento de Ciência dos Alimentos, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Luís Roberto
authors.initials: LR
authors.lastname: Batista
authors.affiliation: Laboratório de Biologia Celular, Instituto Carlos Chagas, Curitiba, PR, Brazil.
authors.forename: Maurilio José
authors.initials: MJ
authors.lastname: Soares
authors.affiliation: Departamento de Ciência dos Alimentos, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Leonardo Milani Avelar
authors.initials: LM
authors.lastname: Rodrigues
authors.affiliation: Faculdade de Ciências de Lisboa, Instituto de Biotecnologia e Bioengenharia, Universidade de Lisboa, Lisboa, Portugal.
authors.forename: Ana Cristina S
authors.initials: AC
authors.lastname: Figueiredo
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D005658:Fungi / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile
keywords: Trypanosoma cruzi; bacteria; fungi; volatile oils