title: Functional characterization of PeIF5B as eIF5B homologue from Pisum sativum.
abstract: We earlier reported 'PeIF5B' as a novel factor from Pisum sativum that has sequence similarity to eIF5B (S. Rasheedi, S. Ghosh, M. Suragani et al., P. sativum contains a factor with strong homology to eIF5B, Gene 399 (2007) 144-151). The main aim of the present study was to perform functional characterization of PeIF5B as an eIF5B homologue from plant system. PeIF5B shows binding to Met - tRNA(f)(Met), hydrolyses GTP and interacts with ribosomes. In vivo growth complementation analysis shows that PeIF5B partially complements its yeast homologue. Interestingly, PeIF5B mainly localizes in the nucleus as confirmed by nuclear localization signal (NLS) prediction, confocal imaging and immunoblots of cellular fractions. Similar to the yeast eIF5B but unlike the human orthologue, PeIF5B is an intron-less gene. This study highlights PeIF5B's role as a functional eIF5B homologue possibly participating in nuclear translation in plant system.
authors.affiliation: Laboratory of Molecular and Cellular Biology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500 001, India.
authors.forename: Sheeba
authors.initials: S
authors.lastname: Rasheedi
authors.affiliation: Molecular Biology Unit, National Institute of Nutrition, Hyderabad 500 007, India.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Suragani
authors.affiliation: Molecular Biology Unit, National Institute of Nutrition, Hyderabad 500 007, India.
authors.forename: Podili
authors.initials: P
authors.lastname: Raviprasad
authors.affiliation: Molecular Biology Unit, National Institute of Nutrition, Hyderabad 500 007, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Biochemistry, University of Hyderabad, Prof. C. R. Rao Road, Hyderabad 500 046, India.
authors.forename: Rajasekhar N V S
authors.initials: RN
authors.lastname: Suragani
authors.affiliation: Department of Biochemistry, University of Hyderabad, Prof. C. R. Rao Road, Hyderabad 500 046, India.
authors.forename: Kolluru V A
authors.initials: KV
authors.lastname: Ramaiah
authors.affiliation: Inflammation Biology and Cell Signaling Laboratory, National Institute of Pathology, Safdarjung Hospital, New Delhi 110029, India. Electronic address: nzehtesham@gmail.com.
authors.forename: Nasreen Z
authors.initials: NZ
authors.lastname: Ehtesham
mesh_terms: D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D018532:Pisum sativum / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D039642:Eukaryotic Initiation Factors; D010940:Plant Proteins; C451459:eukaryotic initiation factor-5B
keywords: Growth complementation analysis; Nuclear localization signal; Nuclear translation; PeIF5B; Pisum sativum; Translation initiation factor