title: Platelet mitochondrial dysfunction of DM rats and DM patients.
abstract: It is known that cardiovascular complications plays important roles in the development of diabetes mellitus (DM) and platelet dysfunction is one of the key reasons which led to microangiopathy. This study was designed to investigate the mitochondria function changes of platelet in DM rats and DM patients. The results showed that the platelets viability, platelet adenosine triphosphate (ATP) content and platelet mitochondrial ATP content of DM rats were lower than that of normal rats; when incubated in vitro for 24 h, platelet number and mitochondrial membrane potential (MMP) of DM rats were lower than that of normal rats, reactive oxygen species (ROS) was higher than that of normal rats. For DM patients, their platelet number and ROS were higher and MMP was lower than those of normal people; when incubated in vitro for 24 h, platelet viability of DM patients was lower than that of normal people. Platelet ultra-microstructures of DM rats and DM patients were abnormal. These results suggested that platelet mitochondrial function of both DM rats and DM patients was impaired when compared to normal rats and normal people, respectively. Platelets may be applied as a biomarker to observe the mitochondrial changes during DM.
authors.affiliation: Department of Anatomy, Medical College of Jinan University Guangzhou 510632, China ; Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China.
authors.forename: Li
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China.
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong Shatin, NT, Hong Kong.
authors.forename: David T
authors.initials: DT
authors.lastname: Yew
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China ; Department of Anatomy, Xinjiang Medical University Urumqi, Xinjiang 830011, China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Guo
keywords: Platelet; diabetic; mitochondria; oxidative stress