title: In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene.
abstract: Tomato yellow leaf curl virus is one of the main diseases affecting tomato production worldwide. Previous studies have shown that Ty-2 is an important resistance gene located between molecular markers C2_At2g28250 (82.3 cM) and T0302 (89.0 cM), and exhibits strong resistance to tomato yellow leaf curl virus in Asia. In this study, Ty-2 candidate genes were subjected to bioinformatic analysis for the sequenced tomato genome. We identified 69 genes between molecular markers C2_At2g28250 and T0302, 22 of which were disease-related resistant genes, including nucleotide binding site-leucine-rich repeat disease resistance genes, protease genes (protein kinase, kinase receptor, and protein isomerase), cytochromes, and transcription factors. Expressed sequence tag analysis revealed that 77.3% (17/22) of candidate disease-resistance genes were expressed, involving 143 expressed sequence tags. Based on full-length cDNA sequence analysis, 7 candidate genes were found, 4 of which were involved in tomato responses to pathogens. Microarray expression analysis also showed that most candidate genes were involved in the tomato responses to multiple pathogens, including fungi, viruses, and bacteria. RNA-seq expression analysis revealed that all candidate genes participated in tomato growth and development.
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China wanhongjian@sina.com.
authors.forename: H J
authors.initials: HJ
authors.lastname: Wan
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Wei
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Wang
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Ruan
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Q J
authors.initials: QJ
authors.lastname: Ye
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Li
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: G Z
authors.initials: GZ
authors.lastname: Zhou
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Yao
authors.affiliation: State key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yang
mesh_terms: D053839:Begomovirus / Q000502:physiology; D002874:Chromosome Mapping; D003198:Computer Simulation; D018076:DNA, Complementary / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056726:Genetic Association Studies; D018551:Solanum lycopersicum / Q000235:genetics* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D017423:Sequence Analysis, RNA
chemical_list: D018076:DNA, Complementary