title: Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition.
abstract: Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A. cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum - shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multi-chromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance.
authors.affiliation: a NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie 514-2392, Japan.|b The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori, Tottori 680-8553, Japan.
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Wako
authors.affiliation: a NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie 514-2392, Japan.
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: a NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie 514-2392, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Tsukazaki
authors.affiliation: a NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie 514-2392, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Ohara
authors.affiliation: a NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie 514-2392, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kojima
authors.affiliation: b The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori, Tottori 680-8553, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Yaguchi
authors.affiliation: c Mikado Kyowa Seed Co. Ltd., 2789-1 Shimo-Ohtaki, Ohtaki, Isumi, Chiba 298-0202, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shimazaki
authors.affiliation: c Mikado Kyowa Seed Co. Ltd., 2789-1 Shimo-Ohtaki, Ohtaki, Isumi, Chiba 298-0202, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Midorikawa
authors.affiliation: c Mikado Kyowa Seed Co. Ltd., 2789-1 Shimo-Ohtaki, Ohtaki, Isumi, Chiba 298-0202, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Sakai
authors.affiliation: b The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori, Tottori 680-8553, Japan.|d Department of Biological and Environmental Science, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi 753-8515, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamauchi
authors.affiliation: b The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori, Tottori 680-8553, Japan.|d Department of Biological and Environmental Science, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi 753-8515, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shigyo
mesh_terms: D001487:Basidiomycota / Q000276:immunology / Q000502:physiology*; D001947:Breeding; D032461:Chromosomes, Plant / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics*; D019697:Onions / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D036226:Seedlings / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D027781:Shallots / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
keywords: Allium cepa; Allium fistulosum; bunching onion; ciboule; lignées d’addition monosomiques; monosomic addition lines; rust resistance; shallot; échalote; ésistance à la rouille