title: Toxic effects of nitenpyram on antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers.
abstract: Nitenpyram is one of the most commonly used neonicotinoid pesticide worldwide and was found to be toxic to non-target aquatic organisms. Therefore, the purpose of this study was to investigate the oxidative stress, changes in the detoxifying system and DNA damage in zebrafish induced by nitenpyram. In the present study, zebrafish (Danio rerio) were exposed to four concentrations (0.6, 1.2, 2.5, and 5.0 mg L(-1)) for 28 d and then sampled in triplicate on days 7, 14, 21 and 28. Superoxide dismutase (SOD) and catalase (CAT) activities were dramatically inhibited at most exposure times compared with the control group, except SOD at low concentration (0.6 mg L(-1)) of nitenpyram and CAT on day 21. This difference is due to the excess reactive oxygen species (ROS) produced and increased malondialdehyde (MDA) content in zebrafish livers. The activity of glutathione S-transferase (GST) increased in in the treatment groups at a higher concentration compared with the control group. We found that nitenpyram exposure could affect the antioxidant enzymes and DNA damage in the exposed zebrafish livers. Additionally, the changes in the antioxidant enzyme activities could be an adaptive response protecting against the toxicity induced by nitenpyram.
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, People's Republic of China. Electronic address: saihongy12@163.com.
authors.forename: Saihong
authors.initials: S
authors.lastname: Yan
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, People's Republic of China. Electronic address: wjh@sdau.edu.cn.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, People's Republic of China. Electronic address: lushzhu@sdau.edu.cn.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Zhu
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, People's Republic of China. Electronic address: chenaimei@126.com.
authors.forename: Aimei
authors.initials: A
authors.lastname: Chen
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, People's Republic of China. Electronic address: jwang@sdau.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000037:antagonists & inhibitors; D004249:DNA Damage; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000494:pharmacology; D000073943:Neonicotinoids; D018384:Oxidative Stress / Q000187:drug effects; D011725:Pyridines / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D000073943:Neonicotinoids; D011725:Pyridines; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; C464843:nitenpyram; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Antioxidant system; Comet assay; GST; MDA; Nitenpyram; ROS