title: Building Triketide α-Pyrone-Producing Yeast Platform Using Heterologous Expression of Sporopollenin Biosynthetic Genes.
abstract: Sporopollenin is a poorly characterized mixed aliphatic and aromatic polymer with ester and ether linkages. Recent studies have reported that α-pyrone polyketide compounds generated by Arabidopsis thaliana, polyketide synthase A (PKSA) and tetraketide α-pyrone reductase 1 (TKPR1), are previously unknown sporopollenin precursors. Here, the yeast Saccharomyces cerevisiae was introduced to test potential sporopollenin biosynthetic pathways in vivo. A PKSA/TKPR1 dual expressor was generated and various chain-length alkyl α-pyrones were identified by GC-MS. The growth rate of the strain containing PKSA/TKPR1 appeared normal. These results indicate that PKSA/TKPR1-expressing yeast would be a starting platform to investigate in vivo sporopollenin metabolism.
authors.affiliation: Biomaterial Laboratory, Samsung Advanced Institute of Technology, Suwon 443-803, Republic of Korea.
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Kim
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001704:Biopolymers / Q000096:biosynthesis*; D053898:Biosynthetic Pathways / Q000235:genetics*; D002338:Carotenoids / Q000096:biosynthesis*; D008401:Gas Chromatography-Mass Spectrometry; D060847:Metabolic Engineering; D011753:Pyrones / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D001704:Biopolymers; D011753:Pyrones; D011994:Recombinant Proteins; C009800:sporopollenin; D002338:Carotenoids
keywords: Sporopollenin monomer; polyketide synthase; tetraketide α-pyrone reductase 1; triketide α-pyrone