title: Neuropathic Pain Phenotype Does Not Involve the NLRP3 Inflammasome and Its End Product Interleukin-1β in the Mice Spared Nerve Injury Model.
abstract: The NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is one of the main sources of interleukin-1β (IL-1β) and is involved in several inflammatory-related pathologies. To date, its relationship with pain has not been studied in depth. The aim of our study was to elucidate the role of NLRP3 inflammasome and IL-1β production on neuropathic pain. Results showed that basal pain sensitivity is unaltered in NLRP3-/- mice as well as responses to formalin test. Spared nerve injury (SNI) surgery induced the development of mechanical allodynia and thermal hyperalgesia in a similar way in both genotypes and did not modify mRNA levels of the NLRP3 inflammasome components in the spinal cord. Intrathecal lipopolysaccharide (LPS) injection increases apoptosis-associated speck like protein (ASC), caspase-1 and IL-1β expression in both wildtype and NLRP3-/- mice. Those data suggest that NLRP3 is not involved in neuropathic pain and also that other sources of IL-1β are implicated in neuroinflammatory responses induced by LPS.
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland; Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland.
authors.forename: Verdad
authors.initials: V
authors.lastname: Curto-Reyes
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland; Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland.
authors.forename: Guylène
authors.initials: G
authors.lastname: Kirschmann
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland; Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland.
authors.forename: Marie
authors.initials: M
authors.lastname: Pertin
authors.affiliation: Department of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors.forename: Stephan K
authors.initials: SK
authors.lastname: Drexler
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland; Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Decosterd
authors.affiliation: Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland.
authors.forename: Marc R
authors.initials: MR
authors.lastname: Suter
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005557:Formaldehyde / Q000633:toxicity; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009437:Neuralgia / Q000139:chemically induced / Q000378:metabolism*; D059348:Peripheral Nerve Injuries / Q000503:physiopathology
chemical_list: D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D005557:Formaldehyde