title: Accelerated healing of cutaneous wounds using phytochemically stabilized gold nanoparticle deposited hydrocolloid membranes.
abstract: Rapid healing of dermatological wounds is of vital importance in preventing infection and reducing post-treatment side-effects. Here we report the therapeutic effects of phytochemically stabilized gold nanoparticles (pAuNPs) coated on a hydrocolloid membrane (HCM) for curing cutaneous wounds. Furthermore, the remedial effects of pAuNPs on skin regeneration and angiogenesis were examined using Sprague Dawley® (SD) rats with skin injuries after a pAuNP-deposited hydrocolloid membrane (pAuNP-HCM) had been applied for 15 days. The rate of wound closure was 4 times faster in the pAuNP-HCM-treated group than in the gauze (GZ)- or HCM-treated groups in the first 5 days. Moreover, wound widths in the pAuNP-HCM-treated group were significantly reduced after 5-15 days of treatment following the injury, compared with the other groups. In addition, a significant increase in collagen expression and a decrease in matrix metalloproteinase (MMP)-1 expression and transforming growth factor (TGF-β1) concentration were observed in the pAuNP-HCM-treated group on day 5. Wound tissue applied with the pAuNP-HCM showed enhancement of vascular endothelial growth factor (VEGF), angiopoietin 1 (Ang-1), and angiopoietin 2 (Ang-2) expression. Furthermore, the activity of superoxide dismutases (SODs) was significantly increased in the skin tissue of the pAuNP-HCM-treated group, compared with the GZ- or HCM-treated groups. It is probable that the accelerated process of wound healing in the injured skin of SD rats via pAuNP-HCM results from the synergistic regulation of angiogenesis and connective tissue formation, as well as the stimulation of antioxidant effects.
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Republic of Korea. dyhwang@pusan.ac.kr.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Jaewook
authors.initials: J
authors.lastname: Lee
authors.forename: Minji
authors.initials: M
authors.lastname: Jang
authors.forename: Moon Hwa
authors.initials: MH
authors.lastname: Kwak
authors.forename: Jun
authors.initials: J
authors.lastname: Go
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Kho
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Song
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Sung
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
mesh_terms: D042702:Angiopoietin-2 / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D047070:Bandages, Hydrocolloid; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D064209:Phytochemicals; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D017695:Soft Tissue Injuries; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry* / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D042702:Angiopoietin-2; D064209:Phytochemicals; D042461:Vascular Endothelial Growth Factor A; D006046:Gold; D020781:Matrix Metalloproteinase 1