title: A non-radioactive method for small RNA detection by northern blotting.
abstract: BACKGROUND
  Small non-coding RNAs are essential regulators of gene expression at the transcriptional and posttranscriptional levels. High-throughput sequencing has revealed thousands of predicted small RNAs; however, only a few of these have been well characterized. Northern blotting is the most convincing method for small RNA validation.
  
  
  RESULTS
  In this study, we improved the Northern blot method by using biotin-labeled probes. miRNAs and siRNAs derived from both Arabidopsis thaliana and Oryza sativa were investigated. The results suggest that this improved method is sensitive and efficient, with approximately 5 μg of total RNA being sufficient for detection. Furthermore, long-term storage of probes labeled in this manner is more convenient, less contaminative and degradative compared with traditional probes.
  
  
  CONCLUSIONS
  This protocol is an alternative strategy for small RNA detection and represents an efficient means of researching small RNAs.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China, huangqi@whu.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.forename: Zhinang
authors.initials: Z
authors.lastname: Mao
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Zhu