title: Human parenchymal and non-parenchymal liver cell isolation, culture and characterization.
abstract: Many reports describing parenchymal liver cell isolation have been published so far. However, recent evidence has clearly demonstrated that non-parenchymal liver cells play an important role in many pathophysiologies of the liver, such as drug-induced liver diseases, inflammation, and the development of liver fibrosis and cirrhosis. In this study, we present an overview of the current methods for isolating and characterizing parenchymal and non-parenchymal liver cells.
authors.affiliation: Charité University Medicine Berlin, Department of General, Visceral, and Transplant Surgery, Augustenburger Platz 1, 13353, Berlin, Germany. georg.damm@charite.de.
authors.forename: Georg
authors.initials: G
authors.lastname: Damm
authors.affiliation: Charité University Medicine Berlin, Department of General, Visceral, and Transplant Surgery, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: Pfeiffer
authors.affiliation: Eberhard Karls University Tübingen, BG Trauma Center, Siegfried Weller Institut, BG-Tübingen, Siegfried Weller Institut, Schnarrenbergstr. 95, 72076, Tübingen, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Burkhardt
authors.affiliation: Department of Pediatrics and Juvenile Medicine, University Hospital Regensburg, Franz-Josef-Strauss Allee 11, 93053, Regensburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Vermehren
authors.affiliation: Eberhard Karls University Tübingen, BG Trauma Center, Siegfried Weller Institut, BG-Tübingen, Siegfried Weller Institut, Schnarrenbergstr. 95, 72076, Tübingen, Germany. andreas.nuessler@gmail.com.
authors.forename: Andreas K
authors.initials: AK
authors.lastname: Nüssler
authors.affiliation: Department of Pediatrics and Juvenile Medicine, University Hospital Regensburg, Franz-Josef-Strauss Allee 11, 93053, Regensburg, Germany. thomas.weiss@ukr.de.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Weiss
keywords: Human hepatocytes; Liver cell culture; Liver cell isolation; Non-parenchymal liver cells; Parenchymal liver cells