title: Downregulation of KIF1B mRNA in hepatocellular carcinoma tissues correlates with poor prognosis.
abstract: OBJECTIVE
  To compare kinesin family member 1B (KIF1B) expression with clinicopathologic parameters and prognosis in hepatocellular carcinoma (HCC) patients.
  
  
  METHODS
  KIF1B protein and mRNA expression was assessed in HCC and paracarcinomatous (PC) tissues from 68 patients with HCC using Western blot and quantitative real-time reverse transcription-PCR, respectively. Student's t-tests were used to analyze relationships between clinicopathologic parameters and KIF1B expression, the Kaplan-Meier method was used to analyze survival outcomes, and the log-rank test was used to compare survival differences between groups.
  
  
  RESULTS
  Mean protein and mRNA levels of KIF1B were similar between HCC and PC tissues. However, HCC tissues with vein invasions had significantly lower KIF1B protein levels compared to those without vein invasions (2.30 ± 0.82 relative units vs 2.77 ± 0.84 relative units, P < 0.05). KIF1B protein levels in HCC tissues from patients with recurrence during the follow-up period were significantly lower than those without recurrence (2.31 ± 0.92 relative units vs 2.80 ± 0.80 relative units, P < 0.05). However, KIF1B protein and mRNA expression in HCC patients was not associated with other clinicopathologic parameters. Ratios of KIF1B mRNA expression in HCC tissues to those in PC tissues were correlated with overall survival (13.5 mo vs 20.0 mo, P < 0.05) and disease-free survival (11.5 mo vs 19.5 mo, P < 0.05).
  
  
  CONCLUSIONS
  Downregulation of KIF1B in HCC tissues is associated with poor prognosis; additional clinical studies are needed to confirm whether KIF1B can serve as a prognostic marker.
authors.affiliation: Song-Zhu Yang, Jian-Tao Wang, Wei-Wei Yu, Qing Liu, Yan-Fang Wu, Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, Shandong Province, China.
authors.forename: Song-Zhu
authors.initials: SZ
authors.lastname: Yang
authors.affiliation: Song-Zhu Yang, Jian-Tao Wang, Wei-Wei Yu, Qing Liu, Yan-Fang Wu, Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, Shandong Province, China.
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Wang
authors.affiliation: Song-Zhu Yang, Jian-Tao Wang, Wei-Wei Yu, Qing Liu, Yan-Fang Wu, Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, Shandong Province, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Yu
authors.affiliation: Song-Zhu Yang, Jian-Tao Wang, Wei-Wei Yu, Qing Liu, Yan-Fang Wu, Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, Shandong Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Song-Zhu Yang, Jian-Tao Wang, Wei-Wei Yu, Qing Liu, Yan-Fang Wu, Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, Shandong Province, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Wu
authors.affiliation: Song-Zhu Yang, Jian-Tao Wang, Wei-Wei Yu, Qing Liu, Yan-Fang Wu, Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, Shandong Province, China.
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Chen
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D016547:Kinesins / Q000032:analysis / Q000235:genetics*; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C091053:KIF1B protein, human; D012333:RNA, Messenger; D016547:Kinesins
keywords: Clinicopathologic correlation; Kinesin family member 1B; Liver cancer; Survival; Tumor progression