title: Cryopreserved Subcutaneous Adipose Tissue for Fat Graft.
abstract: Cryopreservation of subcutaneous white adipose tissue (sWAT) avoids multiple surgeries in patients subjected to reconstructive procedure. Fat grafts were performed subcutaneously on 26 mice treated with fresh (13 mice) or cryopreserved (13 mice) human sWAT. Cytofluorometry for CD marker expression of stem cells, differentiation capability, and in vivo survival of fat grafts were evaluated. In vitro analysis evidenced that cryopreservation did not affect the stem potential of samples. In vivo MRI showed that grafts were well preserved in 13 mice treated with fresh sWAT, whereas in 13 animals treated with thawed fat, graft volumes were strongly reduced after 1 week. Ultrastructural studies performed both on fresh and thawed specimens demonstrated that grafts performed with thawed sWAT are able to store lipids more slowly with respect to grafts performed with fresh sWAT and adipocytes maintained a multilocular appearance. Collected data demonstrated that the protocol of cryopreservation could maintain the regenerative capability of the sWAT, but the rate of reabsorption after fat grafting is higher using cryopreserved sWAT. Maintaining the stem potential of sWAT after cryopreservation is a very important aspect for reconstructive and regenerative medicine. The employment of cryopreserved sWAT represents an interesting goal for surgeons. Surely there is the necessity to improve the protocol of cryopreservation.
  
  
  METHODS
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authors.affiliation: Anatomy and Histology Section, Department of Neurological and Movement Sciences, University of Verona, Strada le Grazie 8, 37134, Verona, Italy, giamaica.conti@univr.it.
authors.forename: Giamaica
authors.initials: G
authors.lastname: Conti
authors.forename: Marcin
authors.initials: M
authors.lastname: Jurga
authors.forename: Donatella
authors.initials: D
authors.lastname: Benati
authors.forename: Paolo
authors.initials: P
authors.lastname: Bernardi
authors.forename: Elisa
authors.initials: E
authors.lastname: Mosconi
authors.forename: Gino
authors.initials: G
authors.lastname: Rigotti
authors.forename: Marjike
authors.initials: M
authors.lastname: Buvé
authors.forename: Kelly
authors.initials: K
authors.lastname: Van Wemmel
authors.forename: Andrea
authors.initials: A
authors.lastname: Sbarbati
mesh_terms: D000368:Aged; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D004195:Disease Models, Animal; D019317:Evidence-Based Medicine; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D008875:Middle Aged; D011897:Random Allocation; D012680:Sensitivity and Specificity; D050151:Subcutaneous Fat / Q000473:pathology / Q000637:transplantation* / Q000648:ultrastructure; D016378:Tissue Transplantation / Q000009:adverse effects / Q000379:methods*