title: Osteogenic Potential of Human Oral-Periosteal Cells (PCs) Isolated From Different Oral Origin: An In Vitro Study.
abstract: The periosteum is a specialized connective tissue containing multipotent stem cells capable of bone formation. In this study, we aimed at demonstrating that human oral periosteal cells derived from three different oral sites (upper vestibule, lower vestibule, and hard palate) represent an innovative cell source for maxillo-facial tissue engineering applications in terms of accessibility and self-commitment towards osteogenic lineage. Periosteal cells (PCs) were isolated from patients with different ages (20-30 yy, 40-50 yy, 50-60 yy); we then analyzed the in vitro proliferation capacity and the bone self-commitment of cell clones culturing them without any osteogenic supplement to support their differentiation. We found that oral PCs, independently of their origin and age of patients, are mesenchymal stem cells with stem cell characteristics (clonogenical and proliferative activity) and that, even in absence of any osteogenic induction, they undertake the osteoblast lineage after 45 days of culture. These results suggest that oral periosteal cells could replace mesenchymal cells from bone marrow in oral tissue-engineering applications.
authors.affiliation: Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Pavia, Italy.|CIT, Tissue Engineering Center, University of Pavia, Pavia, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Ceccarelli
authors.affiliation: Dental school, University of Turin, Turin, Italy.|SHRO Center of Biotechnology, Temple University, Philadelphia, Pennsylvania.
authors.forename: Antonio
authors.initials: A
authors.lastname: Graziano
authors.affiliation: Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Pavia, Italy.|CIT, Tissue Engineering Center, University of Pavia, Pavia, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Benedetti
authors.affiliation: Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Pavia, Italy.|Department of Occupational Medicine, Ergonomy and Disability, Nanotechnology Laboratory, Salvatore Maugeri Foundation, IRCCS, Pavia, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Imbriani
authors.affiliation: Dental school, University of Turin, Turin, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Romano
authors.affiliation: Dental school, University of Turin, Turin, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Ferrarotti
authors.affiliation: Dental school, University of Turin, Turin, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Aimetti
authors.affiliation: Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Pavia, Italy.|CIT, Tissue Engineering Center, University of Pavia, Pavia, Italy.
authors.forename: Gabriella M
authors.initials: GM
authors.lastname: Cusella de Angelis
mesh_terms: D000328:Adult; D001853:Bone Marrow / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D010521:Periosteum / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D055815:Young Adult