title: Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations.
abstract: Understanding sugar-lipid interactions during desiccation and freezing is an important step in the elucidation of cryo- and anhydro-protection mechanisms. We determine sucrose, trehalose, and water concentration distributions in intra-bilayer volumes between opposing dioleoylphosphatidylcholine bilayers over a range of reduced hydrations and sugar concentrations. Stacked lipid bilayers at reduced hydration provide a suitable system to mimic environmental dehydration effects, as well as a suitable system for direct probing of sugar locations by neutron membrane diffraction. Sugar distributions show that sucrose and trehalose both behave as typical uncharged solutes, largely excluded from the lipid bilayers regardless of sugar identity, and with no correlation between sugar distribution and the lipid headgroup position as the hydration is changed. These results are discussed in terms of current opinions about cryo- and anhydro-protection mechanisms.
authors.affiliation: Institute for Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin , Hahn-Meitner-Platz 1, Berlin D-14109, Germany.
authors.forename: Ben
authors.initials: B
authors.lastname: Kent
authors.affiliation: Institute for Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin , Hahn-Meitner-Platz 1, Berlin D-14109, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hauß
authors.affiliation: Institute Laue Langevin , Grenoble 38000, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Demé
authors.affiliation: Institute Laue Langevin , Grenoble 38000, France.
authors.forename: Viviana
authors.initials: V
authors.lastname: Cristiglio
authors.affiliation: Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234, Australia.
authors.forename: Tamim
authors.initials: T
authors.lastname: Darwish
authors.affiliation: Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000, Australia.
authors.forename: Taavi
authors.initials: T
authors.lastname: Hunt
authors.affiliation: Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000, Australia.
authors.forename: Gary
authors.initials: G
authors.lastname: Bryant
authors.affiliation: Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234, Australia.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Garvey
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D013395:Sucrose; C017251:1,2-oleoylphosphatidylcholine