title: A single quantum dot-based biosensor for DNA point mutation assay.
abstract: Sensitive and selective detection of point mutation is essential to molecular biology research and early clinical diagnosis. Here, we demonstrate a single quantum dot (QD)-based biosensor for DNA point mutation assay. In this assay, a mutant target (G/C) remains unchanged after the endonuclease treatment, and the polymerase chain reaction (PCR) may be initiated with the assistance of primers and polymerase, generating a large number of mutant targets. The amplified mutant targets can be captured by biotinylated probes during the process of denaturation and annealing, and Cy5-dGTP may be assembled into the biotinylated probe with the catalysis of polymerase, leading to the formation of Cy5-labeled biotinylated probes. The Cy5-labeled biotinylated probes can be further assembled onto the QD surface to obtain a Cy5-DNA-QD complex, resulting in the generation of fluorescence resonance energy transfer (FRET) between the QD donor and the Cy5 receptor. The mutant targets can be quantitatively evaluated by the measurement of Cy5 counts by total internal reflection fluorescence (TIRF) microscopy. While in the presence of wild-type targets (T/A), no Cy5-dGTP can be assembled into the biotinylated probe due to the presence of a mismatch and consequently no FRET is observed. This single QD-based biosensor exhibits high sensitivity with a detection limit of 5.3 aM (or 32 copies) and can even discriminate as low as 0.01% variant frequency from the mixture of mutant targets and wild-type ones. Importantly, this biosensor can be used for genomic analysis in human lung cancer cells, and may be further applied for an early clinical diagnosis and personalized medicine.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn cyzhang@sdnu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Guichi
authors.initials: G
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques; D019921:Biotinylation; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D005821:Genetic Techniques / Q000295:instrumentation*; D006801:Humans; D057230:Limit of Detection; D008856:Microscopy, Fluorescence; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D004273:DNA, Neoplasm; C085321:cyanine dye 5; D004247:DNA