title: Role of flgA for Flagellar Biosynthesis and Biofilm Formation of Campylobacter jejuni NCTC11168.
abstract: The complex roles of flagella in the pathogenesis of Campylobacter jejuni, a major cause of worldwide foodborne diarrheal disease, are important. Compared with the wild-type, an insertional mutation of the flgA gene (cj0769c) demonstrated significant decrease in the biofilm formation of C. jejuni NCTC11168 on major food contact surfaces, such as polystyrene, stainless steel, and borosilicate glass. The flgA mutant was completely devoid of flagella and non-motile whereas the wild-type displayed the full-length flagella and motility. In addition, the biofilm formation of the wild-type was inversely dependent on the viscosity of the media. These results support that flagellar-mediated motility plays a significant role in the biofilm formation of C. jejuni NCTC11168. Moreover, our adhesion assay suggests that it plays an important role during biofilm maturation after initial attachment. Furthermore, C. jejuni NCTC11168 wild-type formed biofilm with a net-like structure of extracellular fiber-like material, but such a structure was significantly reduced in the biofilm of the flgA mutant. It supports that the extracellular fiber-like material may play a significant role in the biofilm formation of C. jejuni. This study demonstrated that flgA is essential for flagellar biosynthesis and motility, and plays a significant role in the biofilm formation of C. jejuni NCTC11168.
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.|Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea.
authors.forename: Joo-Sung
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.
authors.forename: Changwon
authors.initials: C
authors.lastname: Park
authors.affiliation: Korea Food Research Institute, Seongnam 463-746, Republic of Korea.|Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea.
authors.forename: Yun-Ji
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D016123:Campylobacter jejuni / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005407:Flagella / Q000378:metabolism / Q000502:physiology*; D008124:Locomotion; D016254:Mutagenesis, Insertional; D001678:Organelle Biogenesis
chemical_list: D001426:Bacterial Proteins
keywords: Campylobacter jejuni; biofilm; extracellular fiber-like material; flagella; flgA; motility