title: Multifunctional fluorescent magnetic nanoparticles for lung cancer stem cells research.
abstract: In this paper, a multifunctional peptide-fluorescent-magnetic nanocomposites (Fe₃O₄@PEI@Cy5.5@PEG@HCBP-1 NPs) was synthesized via a layer-by-layer approach for potential application to cancer diagnoses. The multifunctional nanocomposites have great dispersibility and homogeneous particle sizes in aqueous solution. Meanwhile, it has perfect hemocompatibility and satisfying cytocompatibility in a relatively high concentration. Data from in vitro cytotoxicity assay indicated that the nanocomposites could recognize the lung cancer stem cells (CSCs) specifically and enrich the HCBP-1 positive CSCs from H460 tumor xenografts effectively. Additionally, the results of in vivo live fluorescent imaging and magnetic resonance imaging (MRI) showed that the nanocomposites could identify lung CSCs in tumor xenografts. These results suggested that the nanocomposites could be used as a potential cancer diagnostic agent through modifying diverse fluorescence dyes and targeting ligands on its surface.
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Anxin
authors.initials: A
authors.lastname: Wang
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Laboratory of Magnetic Resonance Spectroscopy and Imaging, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China.
authors.forename: Hailu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, PR China. Electronic address: ymzhu2008@sinano.ac.cn.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D045744:Cell Line, Tumor; D005453:Fluorescence; D006461:Hemolysis; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D014411:Neoplastic Stem Cells / Q000473:pathology*; D014961:X-Ray Diffraction
keywords: Cancer stem cell; Magnetic resonance imaging; Multifunctional magnetic nanoparticles; Tumor targeting; in vivo Fluorescent imaging