title: Come together, right now….
abstract: Editing the genome using approaches like TALEN and siRNA are already well tested. The new kid on the block is CRISPR-Cas9. CRISPR-Cas9 is rapidly evolving with impressive refinements for specificity, eliminating off-target effects, and versatility. One can adjust constructs and conditions to produce opposite effects on the genome and for a specific purpose. The nuances of the system, the means to significantly reduce off-targeting, and numerous applications are now emerging rapidly. This B&B commentary looks forward into how the CRISPR-Cas9 tool might serve the CCN field.
authors.affiliation: Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada. hermie@sickkids.ca.
authors.forename: Herman
authors.initials: H
authors.lastname: Yeger
keywords: CCN applications; CRISPR-Cas9; Correcting human disease; Genome editing