title: Enzymatic synthesis of rare sugars with L-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8.
abstract: L-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars D-psicose, D-sorbose, L-tagatose and L-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Zijie
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Wu
authors.affiliation: Division of Mathematics and Science, University of South Carolina Salkehatchie, Walterboro, SC 29488, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.affiliation: China National Research Institute of Food and Fermentation Industries, Beijing 100015, China.
authors.forename: Shenglin
authors.initials: S
authors.lastname: Duan
authors.affiliation: China National Research Institute of Food and Fermentation Industries, Beijing 100015, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: China National Research Institute of Food and Fermentation Industries, Beijing 100015, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: hideki@jiangnan.edu.cn.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakanishi
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: xdgao@jiangnan.edu.cn.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Gao
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D005632:Fructose / Q000096:biosynthesis* / Q000737:chemistry; D006601:Hexoses / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure; D013013:Sorbose / Q000096:biosynthesis* / Q000737:chemistry; D020124:Thermotoga maritima / Q000201:enzymology*
chemical_list: D006601:Hexoses; C003243:psicose; D005632:Fructose; D000446:Aldehyde-Lyases; C022526:rhamnulose-1-phosphate adolase; D013013:Sorbose; C030192:tagatose
keywords: Aldolase; Rare sugar; RhaD; Synthesis; Thermotoga maritima