title: [Sphingosine kinase 1 promotes glioma cell proliferation under hypoxia via calcium signaling].
abstract: OBJECTIVE
  To investigate the role of sphingosine kinase 1 (SphK1) in regulating the proliferation of hypoxia-exposed glioma cells in vitro and explore the possible molecular mechanisms.
  
  
  METHODS
  Human glioblastoma U87MG cells was transfected with specific small interfering RNA (siRNA) constructs targeting SphK1, and the efficiency of SphK1 knockdown was validated by real-time PCR and Western blotting. The cells transfected with SphK1 siRNA and with a negative control siRNA were then exposed to 3% oxygen or 150 µmol/L CoCl2 to induce hypoxia. The cell proliferation and cell cycle changes following the exposure were evaluated with the Cell Counting Kit-8 and flow cytometry, respectively, and the intracellular Ca(2+) changes were monitored using Flou-4/AM under an inverted laser scanning confocal microscope.
  
  
  RESULTS
  SphK1 knockdown significantly reduced hypoxia-induced calcium reflux and suppressed the cell proliferation. Application of OAG, an activator of calcium channels, however, obviously enhanced the cell proliferation under hypoxia.
  
  
  CONCLUSIONS
  SphK1 promotes the proliferation of glioma cells under hypoxia via regulating calcium signaling.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Guangdong Medical College, Dongguan 523808, China.E-mail: hzhang_2011@163.com.
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Linhua
authors.initials: L
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Liu
authors.forename: Huanwen
authors.initials: H
authors.lastname: Tang
mesh_terms: D020013:Calcium Signaling; D002453:Cell Cycle; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005909:Glioblastoma; D005910:Glioma / Q000473:pathology*; D006801:Humans; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D034741:RNA, Small Interfering; D000099157:Sphingosine Kinase
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); D034741:RNA, Small Interfering; D000099157:Sphingosine Kinase