title: Supramolecular Assembly of Artificial Metalloenzymes Based on the Dimeric Protein LmrR as Promiscuous Scaffold.
abstract: Supramolecular anchoring of transition metal complexes to a protein scaffold is an attractive approach to the construction of artificial metalloenzymes since this is conveniently achieved by self-assembly. Here, we report a novel design for supramolecular artificial metalloenzymes that exploits the promiscuity of the central hydrophobic cavity of the transcription factor Lactococcal multidrug resistance Regulator (LmrR) as a generic binding site for planar coordination complexes that do not provide specific protein binding interactions. The success of this approach is manifested in the excellent enantioselectivities that are achieved in the Cu(II) catalyzed enantioselective Friedel-Crafts alkylation of indoles.
authors.affiliation: †Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Bos
authors.affiliation: †Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
authors.forename: Wesley R
authors.initials: WR
authors.lastname: Browne
authors.affiliation: ‡Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands.
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
authors.affiliation: †Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
authors.forename: Gerard
authors.initials: G
authors.lastname: Roelfes
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D013294:Lactococcus lactis; D008667:Metalloproteins / Q000737:chemistry*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary
chemical_list: D001426:Bacterial Proteins; D008667:Metalloproteins