title: A caffeic acid mediated facile synthesis of silver nanoparticles with powerful anti-cancer activity.
abstract: Green synthesis, especially in biological processes, has gained more attention with increasing application of silver nanoparticles (AgNPs) in biomedical fields. However, the biologically synthesized AgNPs have been to be anomalous in size and shape in most cases, as well as exhibiting certain difficulties when used in therapy. We used caffeic acid, a naturally plant polyphenol, to prepare the AgNPs in the current study and also evaluated their anti-cancer activity against the human hepatoma HepG2 cells. Results showed that the AgNPs could rapidly and simply be synthesized using caffeic acid as both a reducing agent and stabilizer. The synthesized AgNPs possessed characteristics of having small size, narrow distribution and high surface negative charge, as well as being stable in aqueous solution. Furthermore, the AgNPs could enter cells and effectively inhibit viability of tumor cells via induction of apoptosis. In conclusion, a caffeic acid mediated facile method was successfully developed to prepare the AgNPs as a potential alternative agent for human hepatoma therapy.
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Guo
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Dou
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou, Nanjing 210096, PR China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China. Electronic address: wlp71@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou, Nanjing 210096, PR China. Electronic address: guning@seu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002109:Caffeic Acids / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D002109:Caffeic Acids; D012834:Silver; C040048:caffeic acid
keywords: Anti-cancer activity; Apoptosis; Caffeic acid; Silver nanoparticles