title: pH and glucose responsive nanofibers for the reversible capture and release of lectins.
abstract: A dual pH and glucose responsive boronic acid containing nanofiber was constructed for the reversible capture and release of lectins. The effects of surface groups and pH values on selective lectin capture were investigated by fluorescence microscopy. Compared to the pristine nanofibrous membrane, glucose and galactose functionalized nanofiber surfaces showed significantly higher capture of ConA and Jacalin, under alkaline conditions. On the other hand, treatment of the modified nanofibers with an acidic solution resulted in the detachment of both the lectins and glycopolymers from the nanofiber surface. As expected, once the glycopolymers are displaced, no lectins were adhered to the nanofiber surface under alkaline conditions. These functional nanofibers can therefore be easily modified and hence can be used for quick removal of selective proteins or toxins from the solution.
authors.affiliation: Department of Chemical and Materials Engineering, University of Alberta, 116 St and 85 Ave, Edmonton, AB T6G 2G6, Canada. narain@ualberta.ca.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kotsuchibashi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Uto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ebara
authors.forename: Takao
authors.initials: T
authors.lastname: Aoyagi
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ravin
authors.initials: R
authors.lastname: Narain
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D012996:Solutions
chemical_list: D001897:Boronic Acids; D037102:Lectins; D037121:Plant Lectins; D012996:Solutions; C043696:jacalin; D003208:Concanavalin A; D005947:Glucose; D005690:Galactose