title: Occurrence of viral pathogens in Penaeus monodon post-larvae from aquaculture hatcheries.
abstract: Viral pathogens appear to exert the most signiﬁcant constraints on the growth and survival of crustaceans under culture conditions. The prevalence of viral pathogens White Spot Syndrome Virus (WSSV), Hepatopancreatic Parvo Virus (HPV), Monodon Baculo Virus (MBV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Penaeus monodon post-larvae was studied. Samples collected from different hatcheries and also samples submitted by farmers from Kerala were analyzed. Out of 104 samples collected, WSSV was detected in 12.5% of the post-larvae samples. Prevalence of concurrent infections by HPV, MBV and WSSV (either dual or triple infection) was present in 60.6% of the total post-larvae tested. Out of the 51 double positives, 98% showed either HPV or IHHNV infection. HPV or IHHNV was detected in 11 post-larval samples showing triple viral infection. This is the first report of IHHNV from India. Result of this study reveals the lack of efficient screening strategies to eradicate viruses in hatchery reared post-larvae.
authors.affiliation: Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Cochin 682029, Kerala, India.
authors.forename: Toms C
authors.initials: TC
authors.lastname: Joseph
authors.affiliation: Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Cochin 682029, Kerala, India.
authors.forename: Roswin
authors.initials: R
authors.lastname: James
authors.affiliation: Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Cochin 682029, Kerala, India.
authors.forename: L
authors.initials: L
authors.lastname: Anbu Rajan
authors.affiliation: Poothuvallil, Dr. Surendran lane, Perumpadappu, Palluruthy P.O, Cochin 682006, Kerala, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Surendran
authors.affiliation: Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Cochin 682029, Kerala, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Lalitha
keywords: Hepatopancreas Parvovirus; Infectious Hypodermal and Hematopoietic Necrosois Virus; Monodon Baculovirus; Penaeus monodon post-larvae; Polymerase Chain Reaction; White Spot Syndrome Virus