title: rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.
abstract: The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.
authors.affiliation: College of Life Sciences, Jilin Agricultural University, Changchun, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Cui
authors.affiliation: College of Agronomy, Jilin Agricultural University, Changchun, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Jilin Agricultural University, Changchun, China.
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Jilin Agricultural University, Changchun, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Dong
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.
authors.forename: F W
authors.initials: FW
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D008027:Light; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D013025:Glycine max / Q000201:enzymology / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
chemical_list: D012273:Ribulose-Bisphosphate Carboxylase