title: Transcriptomic analysis of rice in response to iron deficiency and excess.
abstract: BACKGROUND
  Iron (Fe) is essential micronutrient for plants and its deficiency as well as toxicity is a serious agricultural problem. The mechanisms of Fe deficiency are reasonably understood, however our knowledge about plants response to excess Fe is limited. Moreover, the regulation of small open reading frames (sORFs) in response to abiotic stress has not been reported in rice. Understanding the regulation of rice transcriptome in response to Fe deficiency and excess could provide bases for developing strategies to breed plants tolerant to Fe deficiency as well as excess Fe.
  
  
  RESULTS
  We used a novel rice 110 K microarray harbouring ~48,620 sORFs to understand the transcriptomic changes that occur in response to Fe deficiency and excess. In roots, 36 genes were upregulated by excess Fe, of which three were sORFs. In contrast, 1509 genes were upregulated by Fe deficiency, of which 90 (6%) were sORFs. Co-expression analysis revealed that the expression of some sORFs was positively correlated with the genes upregulated by Fe deficiency. In shoots, 50 (19%) of the genes upregulated by Fe deficiency and 1076 out of 2480 (43%) genes upregulated by excess Fe were sORFs. These results suggest that excess Fe may significantly alter metabolism, particularly in shoots.
  
  
  CONCLUSIONS
  These data not only reveal the genes regulated by excess Fe, but also suggest that sORFs might play an important role in the response of plants to Fe deficiency and excess.
authors.affiliation: Laboratory of Plant Biotechnology, Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan, khurram.bashir@riken.jp.
authors.forename: Khurram
authors.initials: K
authors.lastname: Bashir
authors.forename: Kousuke
authors.initials: K
authors.lastname: Hanada
authors.forename: Minami
authors.initials: M
authors.lastname: Shimizu
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa