title: Hsa-miR-27a is involved in the regulation of CYP3A4 expression in human livers from Chinese Han population.
abstract: OBJECTIVE
  The huge interindividual difference of CYP3A4 expression may contribute to the variability of drug response. Post-transcriptional regulation of CYP3A4 remains elusive although transcriptional regulation has been studied much more clearly. microRNAs (miRNAs) were reported to be one of factors to regulate the expression of CYP3A4 previously.
  
  
  METHODS
  Based on the in silico prediction of 3'-UTR-bindind site of microRNA-27a (miR-27a), the transcriptional and post-transcriptional regulation of miR-27a were investigated through luciferase reporter assay, real-time PCR and immunoblot.
  
  
  RESULTS
  The significantly decrease of CYP3A4 3'-UTR-luciferase activity in human embryonic kidney 293 and Hep3B cells was detected after transfected with plasmid that expressed miRNA-27a in luciferase reporter assay. Correlation study was conducted in human livers (n = 26) and significant correlation has been discovered between miRNA-27a and CYP3A4 mRNA and protein level.
  
  
  CONCLUSIONS
  Together, these findings suggest that miR-27a might be involved in the regulation of CYP3A4 gene expression.
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China.|Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China.|Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China.|Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.|Children's Hospital & Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Children's Hospital & Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, PR China.
authors.forename: Songshan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China.|Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China.|Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Shen
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, PR China.|Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.|Children's Hospital & Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.affiliation: Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.|Children's Hospital & Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Xing
authors.affiliation: Shanghai Genome Pilot Institutes for Genomics & Human Health, Shanghai 200030, PR China.
authors.forename: Shengying
authors.initials: S
authors.lastname: Qin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human
keywords: CYP3A4; Chinese Han population; miR-27a; microRNA; post-transcriptional regulation