title: Differential proteomics of monosodium urate crystals-induced inflammatory response in dissected murine air pouch membranes by iTRAQ technology.
abstract: The precipitation of monosodium urate crystals within joints triggers an acute inflammatory reaction that is the root cause of gout. The inflammation induced by the injection of MSU crystals into the murine air pouch for 1, 3, and 5 h was examined by iTRAQ-based proteomic profiling. The iTRAQ-labeled peptides were fractionated by SCX, basic-RP or solution-IEF, followed by LC-MS/MS analysis. A total of 951 proteins were quantified from the total combined fractions. Among them, 317 proteins exhibited a differential expression, compared to that of the controls at one time point or more. The majority of the differentially expressed proteins were found in the sample after a 5-h MSU treatment. Western blot revealed that the expression levels of cathelin-related antimicrobial peptide and S100A9 were positively correlated with the time-course treated with MSU. Further analysis of GeneGO pathway demonstrated that these differentially expressed proteins are primarily related to the immune-related complement system and the tricarboxylic acid cycle. Moreover, seven genes from the TCA cycle were found to be significantly downregulated at the transcriptional level and its correlation with gout and possible therapeutic applications are worth further investigation. Last, we found that pyruvate carboxylation could be potential targets for antigout treatment.
authors.affiliation: Department of Chemistry, National Taiwan Normal University, Taipei 116, Taiwan.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chiu
authors.affiliation: Department of Life Science, Fu-Jen Catholic University, Taipei, Taiwan.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
authors.affiliation: Department of Life Science, Fu-Jen Catholic University, Taipei, Taiwan.
authors.forename: Tzong-Ta
authors.initials: TT
authors.lastname: Wu
authors.affiliation: Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
authors.forename: Ying-Chu
authors.initials: YC
authors.lastname: Shih
authors.affiliation: Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
authors.forename: Kuo-Kuei
authors.initials: KK
authors.lastname: Huang
authors.affiliation: Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
authors.forename: Ying-Fei
authors.initials: YF
authors.lastname: Tsai
authors.affiliation: Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Hsu
authors.affiliation: Department of Chemistry, National Taiwan Normal University, Taipei 116, Taiwan.
authors.forename: Sung-Fang
authors.initials: SF
authors.lastname: Chen
mesh_terms: D000400:Air Sacs / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid; D005786:Gene Expression Regulation; D007249:Inflammation / Q000139:chemically induced* / Q000378:metabolism; D008297:Male; D051379:Mice; D011506:Proteins / Q000235:genetics*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014527:Uric Acid / Q000494:pharmacology / Q000633:toxicity*
chemical_list: D011506:Proteins; D014527:Uric Acid
keywords: Animal proteomics; Gout; Inflammation; Monosodium urate; Murine air pouch