title: Goat cathelicidin-2 is secreted by blood leukocytes regardless of lipopolysaccharide stimulation.
abstract: It has been reported that goat cathelicidin-2, an antimicrobial peptide, localizes in leukocytes and is present in milk. Here, we examined whether cathelicidin-2 is secreted by leukocytes. Different concentrations (10(5)-10(8) cells/mL) of blood leukocytes were cultured for 0-48 h with or without lipopolysaccharide (LPS). After culture, the concentrations of cathelicidin-2 in the conditioned media were measured. Blood was collected from male goats 0-24 h after the intravenous injection of Escherichia coli O111:B4 LPS. The plasma cathelicidin-2 concentrations were determined and the blood leukocytes immunostained with anti-cathelicidin-2 antibody to calculate the proportion of cathelicidin-2-positive cells in the total leukocytes. When higher concentrations of leukocytes were cultured, the cathelicidin-2 concentrations in the media increased significantly, whereas the addition of LPS to the media caused no further increase. The plasma cathelicidin-2 concentrations did not increase with time after LPS infusion. The proportion of cathelicidin-2-positive cells in the total leukocytes was significantly reduced 1 h after LPS injection compared with that at 0 h, but increased again at 6 h and thereafter. These results suggest that cathlicidin-2 is secreted by leukocytes even without LPS stimulation, whereas LPS may be required for cathelicidin-2-containing leukocytes to be recruited from the blood to tissues showing inflammation.
authors.affiliation: School of Animal Production Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.|Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Supreena
authors.initials: S
authors.lastname: Srisaikham
authors.affiliation: School of Animal Production Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors.forename: Wisitiporn
authors.initials: W
authors.lastname: Suksombat
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Yoshimura
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Isobe
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D006041:Goats; D007962:Leukocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D011498:Protein Precursors / Q000097:blood* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D008070:Lipopolysaccharides; D011498:Protein Precursors; C121625:cathelicidin 2 protein, mammal
keywords: antimicrobial peptide; cathelicidin-2; goat; leukocytes; lipopolysaccharide