title: miR-206 inhibits human laryngeal squamous cell carcinoma cell growth by regulation of cyclinD2.
abstract: OBJECTIVE
  Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressd in many malignancies and crucial to tumorigenesis. Herein, we identified the role and mechanism of miR-206 in laryngeal squamous cell carcinoma (LSCC) growth.
  
  
  METHODS
  Quantitative real-time PCR was performed to detect the relative expression level of miR-206 in LSCC tissues. Crystal violet and flow cytometry were conducted to explore the effects of miR-206 on the proliferation and cell cycle of human LSCC cell line, respectively. The impact of miR-206 overexpression on putative target cyclinD2 were subsequently verified via Western blot. Tumor growth assay was performed to testify the effect of miR-206 on the tumor growth in vivo.
  
  
  RESULTS
  MiR-206 expression was frequently (p < 0.05) down-regulated in LSCC specimens. Overexpression of miR-206 in Hep-2 cell inhibited the proliferation by blocking the G1/S transition as well as suppressed the growth of xenograft tumors in mice, implying that miR-206 functions as a tumour suppressor in the progression of LSCC. Overexpression of miR-206 significantly decreased (p < 0.05) the protein level of cyclinD2, which has previously been identified as a direct targets of miR-206.
  
  
  CONCLUSIONS
  Altogether, our results identify a crucial tumour suppressive role of miR-206 in LSCC growth, at least partly via up-regulation of cyclinD2 protein levels, and suggest that miR-206 might be a candidate prognostic predictor or an anticancer therapeutic target for LSCC patients.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Xinxiang Medical College, Xinxiang, Henan, P. R. China. yu_wenfa@yahoo.com.
authors.forename: W F
authors.initials: WF
authors.lastname: Yu
authors.forename: H M
authors.initials: HM
authors.lastname: Wang
authors.forename: B C
authors.initials: BC
authors.lastname: Lu
authors.forename: G Z
authors.initials: GZ
authors.lastname: Zhang
authors.forename: H M
authors.initials: HM
authors.lastname: Ma
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D056742:Cyclin D2 / Q000502:physiology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C080336:CCND2 protein, human; D056742:Cyclin D2; C546087:MIRN206 microRNA, human; D035683:MicroRNAs