title: Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis.
abstract: OBJECTIVE
  MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.
  
  
  METHODS
  Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann-Whitney test, ANOVA, and operating characteristic (ROC) analysis.
  
  
  RESULTS
  All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (p < 0.05), while the differences of miR-141 and miR-429 between patients and the control group were not statistically significant. Furthermore, all five members were found to be distinctly decreased with the incidence of lymph node metastasis (p < 0.05); When the patients were divided into three groups according to the number of lymph node metastasis (0; 1-3; ≥4), a gradual decrease of miR-200f expression was observed with the increasing number of lymph node metastasis; ROC revealed that miR-200b can differentiate patients with lymph node metastasis from those without lymph node metastasis.
  
  
  CONCLUSIONS
  These observations imply that the down-regulation of miR-200f in human BC is associated with an invasive phenotype, and miR-200b may be useful to estimate the likelihood of the presence of pathologically positive lymph nodes.
authors.affiliation: Department of Breast and Thyroid Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China.
authors.forename: Yunting
authors.initials: Y
authors.lastname: Lin
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Luo
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Du
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China. duanranhui@sklmg.edu.cn.
authors.forename: Ranhui
authors.initials: R
authors.lastname: Duan
mesh_terms: D000328:Adult; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C533896:MIRN200 microRNA, human; D035683:MicroRNAs
keywords: Breast cancer; Clinicopathology; MicroRNA-200; Real-time quantitative PCR