title: Global Gene Expression Analysis for the Assessment of Nanobiomaterials.
abstract: Using global gene expression analysis, the effects of biomaterials and nanomaterials can be analyzed at the genetic level. Even though information obtained from global gene expression analysis can be useful for the evaluation and design of biomaterials and nanomaterials, its use for these purposes is not widespread. This is due to the difficulties involved in data analysis. Because the expression data of about 20,000 genes can be obtained at once with global gene expression analysis, the data must be analyzed using bioinformatics. A method of bioinformatic analysis called gene ontology can estimate the kinds of changes on cell functions caused by genes whose expression level is changed by biomaterials and nanomaterials. Also, by applying a statistical analysis technique called hierarchical clustering to global gene expression data between a variety of biomaterials, the effects of the properties of materials on cell functions can be estimated. In this chapter, these theories of analysis and examples of applications to nanomaterials and biomaterials are described. Furthermore, global microRNA analysis, a method that has gained attention in recent years, and its application to nanomaterials are introduced.
authors.affiliation: Nanotechnology Innovation Station, National Institute for Materials Science, Tsukuba, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hanagata
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D003300:Copper / Q000737:chemistry / Q000633:toxicity; D003603:Cytotoxins / Q000737:chemistry; D003764:Dental Materials / Q000737:chemistry / Q000633:toxicity; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics; D063990:Gene Ontology; D006801:Humans; D035683:MicroRNAs / Q000032:analysis; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D003603:Cytotoxins; D003764:Dental Materials; D035683:MicroRNAs; D003300:Copper; C030973:cupric oxide