title: A high sensitive electrochemical aptasensor for the determination of VEGF(165) in serum of lung cancer patient.
abstract: Herein, a label free electrochemical aptasensor based on ordered mesoporous carbon-gold nanocomposite modified screen printed electrode has been fabricated for the detection of vascular endothelial growth factor (VEGF165) as a tumor marker. The electrochemical behavior of prepared biosensor was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The principle of operation of the proposed aptasensor is based on the changes in the interfacial properties of the electrode due to interaction of the immobilized antiVEGF165 aptamer at the electrode surface with VEGF165 tumor marker in the sample solution, which results in a change in the interfacial charge transfer resistance as detected by electrochemical impedance spectroscopy. The calibration curve for VEGF165 determination was linear over 10.0-300.0 pg mL(-1) with a limit of detection (3σ/S) of 1.0 pg mL(-1). The prepared aptasensor exhibited high sensitivity and good selectivity and reproducibility. The aptasensor was successfully applied to the determination of VEGF165 in serum sample of a lung cancer patient.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran; Department of Chemistry, Bonab Research Center, BNRC, NSTRI, P.O. Box 56515-196, Bonab, East Azarbayjan, Iran. Electronic address: mahmoud.tabizi@gmail.com.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Amouzadeh Tabrizi
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.affiliation: Department of Analytical Chemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Farzin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000302:isolation & purification*
chemical_list: D052157:Aptamers, Nucleotide; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D002244:Carbon; D006046:Gold
keywords: Aptasensor; Label free; Lung cancer; Ordered mesoporous carbon–Au(nano) composite; VEGF(165) tumor marker