title: Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
abstract: Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems. As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge. The studies were conducted at pH 4 at minimal ionic strength, where the βLgA and the pectins are oppositely charged, resulting in an electrostatic attraction to each other. Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration. It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed. Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA. An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
authors.affiliation: Riddet Institute Centre of Research Excellence, Private Bag 11222, Palmerston North 4442, New Zealand.
authors.forename: Amy Y
authors.initials: AY
authors.lastname: Xu
authors.forename: Laurence D
authors.initials: LD
authors.lastname: Melton
authors.forename: Geoffrey B
authors.initials: GB
authors.lastname: Jameson
authors.forename: Martin A K
authors.initials: MA
authors.lastname: Williams
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: McGillivray
mesh_terms: D000818:Animals; D002417:Cattle; D007782:Lactoglobulins / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D007782:Lactoglobulins; D010368:Pectins