title: Competition between Different S-Components for the Shared Energy Coupling Factor Module in Energy Coupling Factor Transporters.
abstract: Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococcus lactis form complexes with the same ECF module. Here, we assayed the uptake of thiamin and niacin in Escherichia coli cells expressing the transporter genes. We demonstrate that the two different S-components compete for the ECF module, and that competition is more efficient in the presence of the transported substrate. The data suggest that binding and release of the S-components is a step in the transport cycle.
authors.affiliation: †Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborg 4, 9747 AG Groningen, The Netherlands.|‡University of Groningen, Zernike Institute for Advanced Materials, Nijenborg 4, 9747 AG Groningen, The Netherlands.
authors.forename: Maria
authors.initials: M
authors.lastname: Majsnerowska
authors.affiliation: †Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborg 4, 9747 AG Groningen, The Netherlands.|‡University of Groningen, Zernike Institute for Advanced Materials, Nijenborg 4, 9747 AG Groningen, The Netherlands.
authors.forename: Josy
authors.initials: J
authors.lastname: Ter Beek
authors.affiliation: †Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborg 4, 9747 AG Groningen, The Netherlands.
authors.forename: Weronika K
authors.initials: WK
authors.lastname: Stanek
authors.affiliation: †Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborg 4, 9747 AG Groningen, The Netherlands.
authors.forename: Ria H
authors.initials: RH
authors.lastname: Duurkens
authors.affiliation: †Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborg 4, 9747 AG Groningen, The Netherlands.|‡University of Groningen, Zernike Institute for Advanced Materials, Nijenborg 4, 9747 AG Groningen, The Netherlands.
authors.forename: Dirk J
authors.initials: DJ
authors.lastname: Slotboom
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D009525:Niacin / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013831:Thiamine / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D011994:Recombinant Proteins; D009525:Niacin; D000255:Adenosine Triphosphate; D013831:Thiamine