title: Analysis of 32 toxic natural substances in herbal products by liquid chromatography quadrupole linear ion trap mass spectrometry.
abstract: In this study, an LC-MS/MS EPI method was developed for simultaneous determination of 32 toxic natural substances in herbal products. The analytes include aconite alkaloids, lobelia alkaloids, solanaceous alkaloids, digitalis steroid glycosides, strychnine, tetrahydropalmatine etc. They werecommonly used in herbal products. The target analytes were extracted from the samples using theQuEChERS method and analysed using AB SCIEX QTRAP 5500 coupled with Agilent HPLC 1260. Thecolumn used was biphenyl reversed phase analytical column. Mobile phase A and B were deionizedwater and methanol respectively, both containing 5mM ammonium formate and 0.1% formic acid. TheMRM-IDA-EPI method enabled quantification and confirmation of the analytes in a single run. The EPIwas used for the qualitative analysis while the MRM was used for the quantitative analysis. Limits ofdetection were determined to be below 10μg/kg for the majority of the analytes. The recoveries forthose commonly detected natural substances were in the acceptable range of 70-120%.
authors.affiliation: Pharmaceutical Laboratory, Health Sciences Authority, 11 Outram Road, 169078, Singapore.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Pharmaceutical Laboratory, Health Sciences Authority, 11 Outram Road, 169078, Singapore.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Quek
authors.affiliation: Pharmaceutical Laboratory, Health Sciences Authority, 11 Outram Road, 169078, Singapore.
authors.forename: Chee-Leong
authors.initials: CL
authors.lastname: Kee
authors.affiliation: Pharmaceutical Laboratory, Health Sciences Authority, 11 Outram Road, 169078, Singapore.
authors.forename: Min-Yong
authors.initials: MY
authors.lastname: Low
authors.affiliation: Pharmaceutical Laboratory, Health Sciences Authority, 11 Outram Road, 169078, Singapore.
authors.forename: Bosco C
authors.initials: BC
authors.lastname: Bloodworth
authors.affiliation: Pharmaceutical Laboratory, Health Sciences Authority, 11 Outram Road, 169078, Singapore. Electronic address: ge_xiao_wei@hsa.gov.sg.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ge
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D015386:Hazardous Substances / Q000032:analysis*; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D015386:Hazardous Substances
keywords: EPI; LC/MS/MS; Library match; Qtrap; Toxic natural substances