title: KAEA (SUDPRO), a member of the ubiquitous KEOPS/EKC protein complex, regulates the arginine catabolic pathway and the expression of several other genes in Aspergillus nidulans.
abstract: The kaeA(KAE1) (suDpro) gene, which was identified in Aspergillus nidulans as a suppressor of proline auxotrophic mutations, encodes the orthologue of Saccharomyces cerevisiae Kae1p, a member of the evolutionarily conserved KEOPS/EKC (Kinase, Endopeptidase and Other Proteins of Small size/Endopeptidase-like and Kinase associated to transcribed Chromatin) complex. In yeast, this complex has been shown to be involved in tRNA modification, transcription, and genome maintenance. In A. nidulans, mutations in kaeA result in several phenotypic effects, the derepression of arginine catabolism genes, and changes in the expression levels of several others, including genes involved in amino acid and siderophore metabolism, sulfate transport, carbon/energy metabolism, translation, and transcription regulation, such as rcoA(TUP1), which encodes the global transcriptional corepressor.
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106 Warsaw, Poland. Electronic address: adzik@igib.uw.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Dzikowska
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Grzelak
authors.affiliation: College of Inter-Faculty Individual Studies in Mathematics and Natural Sciences, University of Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Gawlik
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Szewczyk
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Mrozek
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Borsuk
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Koper
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Empel
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106 Warsaw, Poland; Institute of Experimental Plant Biology, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warsaw, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Szczęsny
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Piłsyk
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pękala
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106 Warsaw, Poland; Centre of New Technologies, University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Weglenski
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005656:Fungal Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D058685:Genetic Pleiotropy; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000502:physiology; D009154:Mutation
chemical_list: D005656:Fungal Proteins; D046912:Multiprotein Complexes; D001120:Arginine
keywords: Arginase; Kae1; Ornithine aminotransferase; rcoA(TUP1)