title: Immunological study of an attenuated Salmonella Typhimurium expressing ApxIA, ApxIIA, ApxIIIA and OmpA of Actinobacillus pleuropneumoniae in a mouse model.
abstract: Salmonella Typhimurium strain expressing the Actinobacillus pleuropneumoniae antigens, ApxIA, ApxIIA, ApxIIIA and OmpA, was previously constructed as a vaccine candidate for porcine pleuropneumonia. This strain was a live attenuated (∆lon∆cpxR∆asd)Salmonella as a delivery host and contained a vector containing asd. An immunological study of lymphocyte proliferation, T-lymphocyte subsets and cytokines in the splenocytes of a mouse model was carried out after stimulation with the candidate Salmonella Typhimurium by intranasal inoculation. The splenic lymphocyte proliferation and the levels of IL-4, IL-6 and IL-12 of the inoculated mice were significantly increased, and the T- and B-cell populations were also elevated. Collectively, the candidate may efficiently induce the Th1- and Th2-type immune responses.
authors.affiliation: Department of Bioactive Material Sciences, and Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Hur
authors.forename: Seong Kug
authors.initials: SK
authors.lastname: Eo
authors.forename: Sang-Youel
authors.initials: SY
authors.lastname: Park
authors.forename: Yoonyoung
authors.initials: Y
authors.lastname: Choi
authors.forename: John Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000189:Actinobacillus Infections / Q000517:prevention & control / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006460:Hemolysin Proteins; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: C085361:ApxII toxin, bacteria; C079721:ApxIII toxin protein, Actinobacillus; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D006460:Hemolysin Proteins; C078167:OMPA outer membrane proteins; C087744:ApxI toxin, Bacteria