title: Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation.
abstract: Nasopharyngeal carcinoma (NPC) is primarily treated by chemoradiation. However, how to promote radiation sensitivity in NPC remains a challenge. Salinomycin is potentially useful for the treatment of cancer. This study aimed to explore the radiosensitivity of salinomycin on human nasopharyngeal carcinoma cell line CNE-2. CNE-2 were treated with salinomycin or irradiation, alone or in combination. The cytotoxicity effects of salinomycin were measured using CCK-8 assay. Clonogenic survival assay was used to evaluate the effects of salinomycin on the radiosensitivity of CNE-2. The changes of cell cycle distribution and apoptosis were assayed using flow cytometry. The expression of Caspase3/Bax/Bal-2 was detected by Western blotting. DNA damage was detected via γ-H2AX foci counting. The results showed that salinomycin induced apoptosis and G2/M arrest, increased Bax and cleaved Caspase3, decreased Bcl-2 expression, and increased the formation of γ-H2AX nuclear foci. These data suggest that salinomycin may be a radiosensitizer for NPC radiotherapy.
authors.affiliation: Department of Radiation Oncology, The First People's Hospital of Zhangjiagang, #68 Jiyangxi Road, Zhangjiagang, Jiangsu province, 215600, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, The First People's Hospital of Zhangjiagang, #68 Jiyangxi Road, Zhangjiagang, Jiangsu province, 215600, China. zuoyunsci@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University, Nantong, 226321, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Guan
authors.affiliation: Department of Radiation Oncology, The First People's Hospital of Zhangjiagang, #68 Jiyangxi Road, Zhangjiagang, Jiangsu province, 215600, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Radiation Oncology, The First People's Hospital of Zhangjiagang, #68 Jiyangxi Road, Zhangjiagang, Jiangsu province, 215600, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D002277:Carcinoma; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor / Q000187:drug effects / Q000528:radiation effects; D002469:Cell Separation; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D006657:Histones / Q000378:metabolism; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011714:Pyrans / Q000737:chemistry*; D011836:Radiation Tolerance; D011838:Radiation-Sensitizing Agents / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000095662:Polyether Polyketides
chemical_list: D053148:Caspase 3; D006657:Histones; D019253:Proto-Oncogene Proteins c-bcl-2; D011714:Pyrans; D011838:Radiation-Sensitizing Agents; D051028:bcl-2-Associated X Protein; C494826:BAX protein, human; C000595707:BCL2 protein, human; C468783:H2AX protein, human; C010327:salinomycin; D000095662:Polyether Polyketides; C505407:CASP3 protein, human
keywords: Nasopharyngeal carcinoma; Radiosensitization; Salinomycin