title: Sensitivity and robustness enhancements by using a V-shape ion funnel in FTICR-MS.
abstract: In this paper, a new configuration of the ion funnel interface (i.e., V-shape ion funnel (V-IF)) for high ion transmission efficiency and robustness enhancement was developed and implemented on FTICR-MS. The performance of the V-IF was compared with that of a home-built orthogonal ion funnel. An order of magnitude of improvement in sensitivity was achieved for various peptides and proteins. The performance of the instrument was maintained for a long period by neutral molecule removal. Other ion transmission patterns, such as gentle ion transmission, adduct ion removal, and radio frequency (RF)-driven collision induced dissociation (CID), was also realized in V-IF by varying the RF potentials. V-IF is believed to be a novel ion guide that has promising applications in mass spectrometry.
authors.affiliation: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.|§Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.
authors.forename: Liulin
authors.initials: L
authors.lastname: Deng
authors.affiliation: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.|‡Shandong Academy of Sciences, Jinan, Shandong, 250014, P. R. China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors.forename: Wan
authors.initials: W
authors.lastname: Li
authors.affiliation: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Wang
authors.affiliation: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors.forename: Yiling Elaine
authors.initials: YE
authors.lastname: Wong
authors.affiliation: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors.forename: T-W Dominic
authors.initials: TW
authors.lastname: Chan
mesh_terms: D057230:Limit of Detection; D013058:Mass Spectrometry / Q000295:instrumentation*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D011506:Proteins