title: FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL.
abstract: Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitro resistance to azoles was not detected, except for C. krusei. The two primers, OPA09 and OPB11, were able to distinguish different species. Isolates of C. albicans and C. parapsilosis complex species presented six and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing the genetic variability of the isolates of those species. It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole. High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker.
authors.affiliation: FAMED, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Ralciane de Paula
authors.initials: Rde P
authors.lastname: Menezes
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Joseane Cristina
authors.initials: JC
authors.lastname: Ferreira
authors.affiliation: Clinical Hospital of Uberlândia, UFU, Minas Gerais, Brazil.
authors.forename: Walkiria Machado
authors.initials: WM
authors.lastname: de Sá
authors.affiliation: Clinical Hospital of Uberlândia, UFU, Minas Gerais, Brazil.
authors.forename: Tomaz de Aquino
authors.initials: Tde A
authors.lastname: Moreira
authors.affiliation: Clinical Hospital of Uberlândia, UFU, Minas Gerais, Brazil.
authors.forename: Lucivânia Duarte Silva
authors.initials: LD
authors.lastname: Malvino
authors.affiliation: Faculty of Mathematics, UFU, Uberlândia, Minas Gerais, Brazil.
authors.forename: Lucio Borges
authors.initials: LB
authors.lastname: de Araujo
authors.affiliation: FAMED, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Denise Von Dolinger de Brito
authors.initials: DV
authors.lastname: Röder
authors.affiliation: UFU, Technical School of Health, Uberlândia, Minas Gerais, Brazil.
authors.forename: Mario Paulo Amante
authors.initials: MP
authors.lastname: Penatti
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Regina Celia
authors.initials: RC
authors.lastname: Candido
authors.affiliation: FAMED, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Reginaldo Dos Santos
authors.initials: Rdos S
authors.lastname: Pedroso
mesh_terms: D000369:Aged, 80 and over; D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001938:Brazil; D002175:Candida / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017964:Itraconazole / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D063128:Tertiary Health Care
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole