title: An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides.
abstract: A sustainable and scalable process for the production of a new mixture of fructooligosaccharides (FOS) was developed using a continuous-flow approach based on an immobilized whole cells-packed bed reactor. The technological transfer from a classical batch system to an innovative flow environment allowed a significant improvement of the productivity. Moreover, the stability of this production system was ascertained by up to 7 days of continuous working. These results suggest the suitability of the proposed method for a large-scale production of the desired FOS mixture, in view of a foreseeable use as a novel prebiotic preparation.
authors.affiliation: Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Zambelli
authors.affiliation: Department of Pharmaceutical Sciences (DISFARM), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy. Electronic address: lucia.tamborini@unimi.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Tamborini
authors.affiliation: Department of Pharmaceutical Sciences (DISFARM), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
authors.forename: Samuele
authors.initials: S
authors.lastname: Cazzamalli
authors.affiliation: Department of Pharmaceutical Sciences (DISFARM), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Pinto
authors.affiliation: Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Arioli
authors.affiliation: Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Balzaretti
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Plou
authors.affiliation: Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Fernandez-Arrojo
authors.affiliation: Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Molinari
authors.affiliation: Department of Pharmaceutical Sciences (DISFARM), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Conti
authors.affiliation: Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy. Electronic address: diego.romano@unimi.it.
authors.forename: Diego
authors.initials: D
authors.lastname: Romano
mesh_terms: D005434:Flow Cytometry; D009844:Oligosaccharides / Q000737:chemistry*; D056692:Prebiotics
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; C116580:fructooligosaccharide
keywords: Cladosporium cladosporioides; Dried alginate entrapped enzymes (DALGEE); Flow chemistry; Fructooligosaccharides; Prebiotics