title: Effect of an osmotic stress on multicellular aggregates.
abstract: There is increasing evidence that multicellular structures respond to mechanical cues, such as the confinement and compression exerted by the surrounding environment. In order to understand the response of tissues to stress, we investigate the effect of an isotropic stress on different biological systems. The stress is generated using the osmotic pressure induced by a biocompatible polymer. We compare the response of multicellular spheroids, individual cells and matrigel to the same osmotic perturbation. Our findings indicate that the osmotic pressure occasioned by polymers acts on these systems like an isotropic mechanical stress. When submitted to this pressure, the volume of multicellular spheroids decreases much more than one could expect from the behavior of individual cells.
authors.affiliation: Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Unité Mixte de Recherche 168, 75231 Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Monnier
authors.affiliation: Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Unité Mixte de Recherche 168, 75231 Paris, France.
authors.forename: Morgan
authors.initials: M
authors.lastname: Delarue
authors.affiliation: Laboratoire Interdisciplinaire de Physique, Centre National de la Recherche Scientifique, Université Joseph Fourier, Unité Mixte de Recherche 5588, 38402 St. Martin d'Hères, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Brunel
authors.affiliation: Laboratoire Interdisciplinaire de Physique, Centre National de la Recherche Scientifique, Université Joseph Fourier, Unité Mixte de Recherche 5588, 38402 St. Martin d'Hères, France.
authors.forename: Monika E
authors.initials: ME
authors.lastname: Dolega
authors.affiliation: Laboratoire Interdisciplinaire de Physique, Centre National de la Recherche Scientifique, Université Joseph Fourier, Unité Mixte de Recherche 5588, 38402 St. Martin d'Hères, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Delon
authors.affiliation: Laboratoire Interdisciplinaire de Physique, Centre National de la Recherche Scientifique, Université Joseph Fourier, Unité Mixte de Recherche 5588, 38402 St. Martin d'Hères, France. Electronic address: giovanni.cappello@ujf-grenoble.fr.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cappello
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D002449:Cell Aggregation; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D048429:Cell Size; D040542:Mechanotransduction, Cellular; D051379:Mice; D009997:Osmotic Pressure; D018874:Spheroids, Cellular / Q000502:physiology*
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels
keywords: Cancer growth; Multicellular systems; Osmotic pressure; Tissue mechanics