title: Characterization of l-Digitoxosyl-phenanthroviridin from Streptomyces venezuelae ISP5230.
abstract: The jadomycin-derived compound l-digitoxosyl-phenanthroviridin was isolated from fermentations of Streptomyces venezuelae ISP5230 grown in nutrient-deficient media with l-lysine as the sole nitrogen source. Structural elucidation was accomplished using a combination of high-resolution MS, LC-MS/MS, and 1D- and 2D-NMR. The compound was evaluated against the National Cancer Institute (NCI) 60 human tumor cell line screen in both the one-dose and five-dose screens, and cytotoxicity was compared to a small library of jadomycin analogues to probe the structure-activity relationship.
authors.affiliation: Department of Chemistry, Dalhousie University , Halifax, Nova Scotia, Canada , B3H 4R2.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Robertson
authors.affiliation: Department of Chemistry, Dalhousie University , Halifax, Nova Scotia, Canada , B3H 4R2.
authors.forename: Camilo F
authors.initials: CF
authors.lastname: Martinez-Farina
authors.affiliation: Institute for Marine Biosciences, National Research Council of Canada , Halifax, Nova Scotia, Canada , B3H 3Z1.
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Syvitski
authors.affiliation: Department of Chemistry, Dalhousie University , Halifax, Nova Scotia, Canada , B3H 4R2.|College of Pharmacy, Dalhousie University , Halifax, Nova Scotia, Canada , B3H 1X7.
authors.forename: David L
authors.initials: DL
authors.lastname: Jakeman
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D053119:Benzophenanthridines / Q000737:chemistry / Q000302:isolation & purification*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007546:Isoquinolines / Q000737:chemistry / Q000302:isolation & purification*; D008239:Lysine / Q000378:metabolism; D015394:Molecular Structure; D054547:National Cancer Institute (U.S.); D019906:Nuclear Magnetic Resonance, Biomolecular; D013302:Streptomyces / Q000737:chemistry*; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry; D014481:United States
chemical_list: D000900:Anti-Bacterial Agents; D053119:Benzophenanthridines; D007546:Isoquinolines; C000602989:l-digitoxosyl-phenanthroviridin; C081750:jadomycin B; D008239:Lysine