title: The in vitro protective effect of salicylic acid against paclitaxel and cisplatin-induced neurotoxicity.
abstract: Paclitaxel (PAC) and cisplatin (CIS) are two established chemotherapeutic drugs used in combination for the treatment of various solid tumors. However, the usage of PAC and CIS are limited because of the incidence of their moderate or severe neurotoxic side effects. In this study, we aimed to assess the protective role of salicylic acid (SA) against neurotoxicity caused by PAC and CIS. For this purpose, newborn Sprague Dawley rats were decapitated in sterile atmosphere and primary cortex neuron cultures were established. On the 10th day SA was added into culture plates. PAC and CIS were added on the 12th day. The cytotoxicity was determined by using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Oxidative alterations were assessed using total antioxidant capacity and total oxidative stress assays in rat primary neuron cell cultures. It was shown that both concentrations of PAC and CIS treatments caused neurotoxicity. Although SA decreased the neurotoxicity by CIS and PAC, it was more effective against the toxicity caused by CIS rather than the toxicity caused by PAC. In conclusion it was clearly revealed that SA decreased the neurotoxic effect of CIS and PAC in vitro.
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, Kafkas University, Kars, Turkey.
authors.forename: Damla
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Erzurum, Turkey. ahmeth@atauni.edu.tr.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Hacımuftuoglu
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
authors.forename: Abdulgani
authors.initials: A
authors.lastname: Tatar
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Sciences, Erzurum Technical University, Erzurum, Turkey.|Department of Pharmacy, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
authors.forename: Hasan
authors.initials: H
authors.lastname: Turkez
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
authors.forename: Basak
authors.initials: B
authors.lastname: Togar
keywords: Antioxidant capacity; Cisplatin; Neurotoxicity; Oxidative stress; Paclitaxel; Salicylic acid