title: Synthesis and characterization of dendro-PLGA nanoconjugate for protein stabilization.
abstract: The present investigation was aimed to develop the dendro-PLGA nanoconjugate (DPNC) for protection of insulin from degradation as well as its sustained release from nano-formulation. DPNC formulation was synthesized by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) coupling reaction and therapeutic efficacy of encapsulated protein (insulin) was measured by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR) and circular dichorism (CD) spectroscopy. We have also demonstrated the ability of DPNC formulation to prevent the native conformation of insulin within the system by comparing the amount of free protein with the protein extracted from this system. Stability study further revealed that as compared to free protein, DPNC formulation was more efficient to stabilize the protein. Additionally, in vivo data of protein loaded system in rats showed that DPNC formulation can able to maintain the native structure of insulin and hence retain therapeutic efficacy of protein. The novel dendro-PLGA nanoconjugate not only stabilize the insulin but also work as sustained release reservoir for insulin which reduces the frequency of dosage and side effect associate with denatured protein.
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour Central University, Sagar, Madhya Pradesh 470003, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour Central University, Sagar, Madhya Pradesh 470003, India; Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy & Health Sciences, Wayne State University, Detroit, MI 48201, USA. Electronic address: prashant_pharmacy04@rediffmail.com.
authors.forename: Prashant
authors.initials: P
authors.lastname: Kesharwani
authors.affiliation: Center for Nanobioscience, Agharkar Research Institute, Pune 411004, India.
authors.forename: Virendra
authors.initials: V
authors.lastname: Gajbhiye
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour Central University, Sagar, Madhya Pradesh 470003, India. Electronic address: jnarendr@yahoo.co.in.
authors.forename: Narendra K
authors.initials: NK
authors.lastname: Jain
mesh_terms: D000818:Animals; D050091:Dendrimers / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D058726:Nanoconjugates; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011126:Polypropylenes / Q000737:chemistry*; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D050091:Dendrimers; D058726:Nanoconjugates; D011126:Polypropylenes; D011506:Proteins; C443641:poly(propyleneimine); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Insulin; PLGA; PPI dendrimer; Protein; Stability