title: The basophil activation test: a sensitive test in the diagnosis of allergic immediate hypersensitivity to pristinamycin.
abstract: Immediate hypersensitivity (IHS) reactions to macrolides and to macrolide-derived antibiotics like pristinamycin are uncommon. In this context, there is little data available to appreciate the true value of biological tools regarding the diagnosis of immediate allergy to pristinamycin. Here we assess the clinical usefulness of the basophil activation test (BAT) to differentiate allergic from nonallergic IHS to pristinamycin. Thirty-six patients were tested with skin tests as the gold standard and BAT. The BAT achieved a sensitivity of 76% and a specificity of 100%, implying an absence of false positive results. Multicenter studies remain to be performed to better define the sensitivity, specificity and interlaboratory variation of BAT in the diagnosis of allergy to pristinamycin and macrolides.
authors.affiliation: Laboratoire d'Immunologie, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, Lyon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Viel
authors.forename: Lorna
authors.initials: L
authors.lastname: Garnier
authors.forename: Elodie
authors.initials: E
authors.lastname: Joly
authors.forename: Paul
authors.initials: P
authors.lastname: Rouzaire
authors.forename: Audrey
authors.initials: A
authors.lastname: Nosbaum
authors.forename: Pauline
authors.initials: P
authors.lastname: Pralong
authors.forename: Amélie
authors.initials: A
authors.lastname: Faudel
authors.forename: Catherine
authors.initials: C
authors.lastname: Rioufol
authors.forename: Françoise
authors.initials: F
authors.lastname: Bienvenu
authors.forename: Jacques
authors.initials: J
authors.lastname: Bienvenu
authors.forename: Frédéric
authors.initials: F
authors.lastname: Berard
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D015549:Basophil Degranulation Test / Q000379:methods* / Q000706:statistics & numerical data; D016022:Case-Control Studies; D003663:Decision Trees; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000209:etiology* / Q000276:immunology; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000209:etiology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D025762:Pristinamycin / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D025762:Pristinamycin