title: Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues.
abstract: We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.
authors.affiliation: 1] Medical Scientist Training Program, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA [2] Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA [3] The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Scoville
authors.affiliation: The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Keller
authors.affiliation: The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Cheng
authors.affiliation: The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Zhang
authors.affiliation: 1] Center for Biostatistics, The Ohio State University, Columbus, Ohio 43210 [2] The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1] Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA [2] The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Caligiuri
authors.affiliation: 1] Division of Hematopathology, Department of Pathology, The Ohio State University, Columbus, Ohio 43210, USA [2] The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
authors.forename: Aharon G
authors.initials: AG
authors.lastname: Freud
mesh_terms: D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*