title: Influence of propofol on oxidative-antioxidative system parameters in peripheral organs of rats with Parkinson disease.
abstract: Propofol (2,6-diisopropylphenol) is a popular anaesthetic agent with antioxidant properties. The aim of the study was to assess the oxidant-antioxidant system parameters of particular organs (liver, kidney, heart, and lungs) in response to propofol administered to rats with Parkinson's disease and to healthy ones. The experiment was performed using 32 Wistar rats divided into four groups (8 rats each). The groups were as follows: 1 control, 2 Parkinson's disease, 3 control with propofol, 4 Parkinson's disease with propofol. Propofol was administered at a dose of 60 mg/kg body weight/IP, 60 minutes before decapitation. Animals were sacrificed and livers, kidneys, hearts and lungs were obtained for further biochemical analyses. The concentration of malondialdehyde (MDA), glutathione reductase (GR) activity, glutathione peroxidase (GPx) activity, glutathione S-transferase (GST) activity and catalase (CAT) activity were determined. In group 4 compared to group 2 there was observed a significant decrease in the MDA level in liver (71%), kidneys (51%) and heart (12%), increased GR activity in lungs (48%) and heart (34%), and increased CAT activity in liver (104%). In group 3 compared to group 1 there was a significant decrease in MDA level in kidneys (67%) and lungs (14%) and increased GR activity in heart (31%), liver (29%) and lungs (21%). Propofol can prevent or reduce damage caused by reactive oxygen species (ROS) by stimulating activity of antioxidative enzymes and inhibiting lipid peroxidation. Additionally, the response of tissues to administered propofol is different in Parkinson's disease and in healthy individuals.
authors.affiliation: Medical University of Silesia, School of Medicine with the Division of Dentistry, Department of Biochemistry, Zabrze, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Romuk
authors.affiliation: Medical University of Silesia, School of Medicine with the Division of Dentistry, Department of Biochemistry, Zabrze, Poland.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Szczurek
authors.affiliation: Medical University of Silesia, School of Public Health, Department of Toxicology and Addiction, Zabrze, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Nowak
authors.affiliation: Medical University of Silesia, School of Medicine with the Division of Dentistry, Department of Biochemistry, Zabrze, Poland.
authors.forename: Iga
authors.initials: I
authors.lastname: Kwiecień
authors.affiliation: Medical University of Silesia, School of Medicine with the Division of Dentistry, Department of Biochemistry, Zabrze, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Stolecka
authors.affiliation: Medical University of Silesia, School of Medicine with the Division of Dentistry, Department of Biochemistry, Zabrze, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Birkner
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010300:Parkinson Disease / Q000378:metabolism*; D015742:Propofol / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D015742:Propofol