title: Identification of a New Tetracycline Resistance Determinant tet47 from Fish Intestine.
abstract: To better understand food safety risks, functional genomic analysis was conducted to identify undescribed antibiotic resistance genes in fish samples from an aquaculture fish farm in Ohio. A fosmid genomic library from pooled DNA of antibiotic-resistant isolates was used to screen for resistance genes against tetracycline (Tet). A new Tet-resistant determinant designated as tet 47 was identified, with the original hosts being Providencia spp. from fish intestine. The new gene was also found to confer Tet resistance in Escherichia coli. Fish and byproducts were shown to be possible carriers that may disseminate new, functional, and potentially transmissible antibiotic resistance determinants through food, feed, and environmental contacts.
authors.affiliation: Department of Food Science and Technology, 2015 Fyffe Court, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Food Science and Technology, 2015 Fyffe Court, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Technology, 2015 Fyffe Court, The Ohio State University, Columbus, Ohio 43210, USA; 2Department of Microbiology, 484 West 12th Avenue, The Ohio State University, Columbus, Ohio 43210, USA. wang.707@osu.edu.
authors.forename: Hua H
authors.initials: HH
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D019295:Computational Biology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005399:Fishes; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D015723:Gene Library; D023281:Genomics; D007422:Intestines / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009820:Ohio; D010802:Phylogeny; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial