title: Caryocar brasiliense camb protects against genomic and oxidative damage in urethane-induced lung carcinogenesis.
abstract: The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective effects against oxidative damage in lung carcinogenesis. In the present study, we evaluated the role of pequi fruit against urethane-induced DNA damage and oxidative stress in forty 8-12 week old male BALB/C mice. An in vivo comet assay was performed to assess DNA damage in lung tissues and changes in lipid peroxidation and redox cycle antioxidants were monitored for oxidative stress. Prior supplementation with pequi oil or its extract (15 µL, 60 days) significantly reduced urethane-induced oxidative stress. A protective effect against DNA damage was associated with the modulation of lipid peroxidation and low protein and gene expression of nitric oxide synthase. These findings suggest that the intake of pequi fruit might protect against in vivo genotoxicity and oxidative stress.
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: N B R
authors.initials: NB
authors.lastname: Colombo
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: M P
authors.initials: MP
authors.lastname: Rangel
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: V
authors.initials: V
authors.lastname: Martins
authors.affiliation: Laboratório de Poluição Atmosférica, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: M
authors.initials: M
authors.lastname: Hage
authors.affiliation: Departamento de Stress Oxidativo, Universidade do Rio Grande do Sul, Rio Grande do Sul, RS, BR.
authors.forename: D P
authors.initials: DP
authors.lastname: Gelain
authors.affiliation: Departamento de Emergência Clínica, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: D F
authors.initials: DF
authors.lastname: Barbeiro
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasília, Brasília, DF, BR.
authors.forename: C K
authors.initials: CK
authors.lastname: Grisolia
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: E R
authors.initials: ER
authors.lastname: Parra
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: V L
authors.initials: VL
authors.lastname: Capelozzi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D063646:Carcinogenesis; D002273:Carcinogens; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D020448:Ericales / Q000737:chemistry*; D016678:Genome; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008175:Lung Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D014520:Urethane
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D014520:Urethane