title: Buccal adhesive nanofibers containing human growth hormone for oral mucositis.
abstract: Due to a lack of proper drug carriers to deliver treatments for mucositis, many cancer patients suffer from oral mucositis caused by chemotherapy and radiotherapy. We prepared a double-layered electrospun nanofibrous sheets composed of Eudragit and chitosan to accelerate the healing rate of oral mucous ulcer. Human growth hormone (hGH) and Eudragit in a mixture of dimethylacetamide and ethanol were co-electrospun to nanofibrous sheets. The electrospun fibrous mat was subsequently layered with chitosan by a dip-coating method. Chitosan-layered sheets showed attenuated mass erosion while uncoated sheets were instantly melted at the physiological condition. The released hGH was trapped on the chitosan layer by the ionic interaction between positively charged chitosan and negatively charged hGH, and a large number of entrapped proteins remained on the SIS membrane due to the muco-adhesive properties of chitosan. hGH-incorporated sheets significantly increased proliferation of human dermal fibroblasts. In vivo study employing oral ulcers in dogs, the ulcers dressed with chitosan-layered sheets showed enhanced wound recovery and the chitosan layers on the sheet greatly assisted prolonged recovery. Therefore, chitosan-layered Eudragit nanofibrous sheets can be potentially applied to developing muco-adhesive wound dressing materials with pH-dependent drug release by adjusting the thickness of chitosan sheath on the sheets. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1396-1406, 2016.
authors.affiliation: Department of Biomedical Materials Engineering, School of Bioscience and Bioengineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea.|Institute of Bioscience and Bioengineering, Kangwon National University, Republic of Korea.
authors.forename: Ji Suk
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Section of Small Animal Internal Medicine, College of Veterinary Medicine, Kangwon National University, Republic of Korea.
authors.forename: Suk-Hee
authors.initials: SH
authors.lastname: Han
authors.affiliation: Section of Small Animal Internal Medicine, College of Veterinary Medicine, Kangwon National University, Republic of Korea.
authors.forename: Changbaig
authors.initials: C
authors.lastname: Hyun
authors.affiliation: Department of Biomedical Materials Engineering, School of Bioscience and Bioengineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea. hsyoo@kangwon.ac.kr.|Institute of Bioscience and Bioengineering, Kangwon National University, Republic of Korea. hsyoo@kangwon.ac.kr.
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D019382:Human Growth Hormone / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D009061:Mouth Mucosa; D057139:Nanofibers / Q000737:chemistry*; D013280:Stomatitis / Q000628:therapy*; D014014:Tissue Adhesives / Q000737:chemistry / Q000494:pharmacology
chemical_list: D014014:Tissue Adhesives; D019382:Human Growth Hormone; D048271:Chitosan
keywords: Eudragit L100; chitosan; human growth hormone; muco-adhesiveness; nanofibrous sheet