title: Targeted Next-Generation Sequencing Reveals Hot Spots and Doubly Heterozygous Mutations in Chinese Patients with Familial Cardiomyopathy.
abstract: As a common cardiac disease mainly caused by gene mutations in sarcomeric cytoskeletal, calcium-handling, nuclear envelope, desmosomal, and transcription factor genes, inherited cardiomyopathy is becoming one of the major etiological factors of sudden cardiac death (SCD) and heart failure (HF). This disease is characterized by remarkable genetic heterogeneity, which makes it difficult to screen for pathogenic mutations using Sanger sequencing. In the present study, three probands, one with familial hypertrophic cardiomyopathy (FHCM) and two with familial dilated cardiomyopathy (FDCM), were recruited together with their respective family members. Using next-generation sequencing technology (NGS), 24 genes frequently known to be related to inherited cardiomyopathy were screened. Two hot spots (TNNI3-p.Arg145Gly, and LMNA-p.Arg190Trp) and double (LMNA-p.Arg190Trp plus MYH7-p.Arg1045His) heterozygous mutations were found to be highly correlated with familial cardiomyopathy. FDCM patients with doubly heterozygous mutations show a notably severe phenotype as we could confirm in our study; this indicates that the double mutations had a dose effect. In addition, it is proposed that genetic testing using NGS technology can be used as a cost-effective screening tool and help guide the treatment of patients with familial cardiomyopathy particularly regarding the risk of family members who are clinically asymptomatic.
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: The Cardiac Department, The First Hospital of Yunnan Province, Kunming 650034, China.
authors.forename: Yun-Mei
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: The Cardiac Department, The First Hospital of Yunnan Province, Kunming 650034, China.
authors.forename: Xiao-Xue
authors.initials: XX
authors.lastname: Ding
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Yu-Zhu
authors.initials: YZ
authors.lastname: Song
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: A-Mei
authors.initials: AM
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: The Cardiac Department, The First Hospital of Yunnan Province, Kunming 650034, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Jia-Huan
authors.initials: JH
authors.lastname: Ding
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Diagnosis in Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Xia
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D024722:Cardiac Myosins / Q000235:genetics; D002311:Cardiomyopathy, Dilated / Q000000981:diagnostic imaging / Q000235:genetics*; D002681:China; D004252:DNA Mutational Analysis; D005260:Female; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D034904:Lamin Type A / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics; D010641:Phenotype; D019210:Troponin I / Q000235:genetics; D014463:Ultrasonography; D055815:Young Adult
chemical_list: C494847:LMNA protein, human; D034904:Lamin Type A; C501641:MYH7 protein, human; D019210:Troponin I; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains