title: [Effect of Qige Powder on Angiogenesis Induced by Esophageal Cancer Cell Line Eca-9706].
abstract: OBJECTIVE
  To study the effect Qige powder on esophageal cancer angiogenesis.
  
  
  METHODS
  Inhibitive effect of Qige powder ethanol extract on proliferation of Esophageal cancer cell line Eca-9706 was detected by MTT assay. The Eca-9706 cells conditioned medium in Qige powder IC50 concentration were collected. Angiogenesis, as well as proliferation, migration and tube formation of human umbilical vein endothelial cells were observed by Chick embryo chorioallantoic membrane model(CAM), MTT assay, the migration and tube formation assay. VEGF and IL-6 contents in culture medium supernatant of Eca-9706 cells and human umbilical vein endothelial cells were detected by ELISA.
  
  
  RESULTS
  Qige powder ethanol extract could inhibit the proliferation of Eca-9706 cells, with a certain dose-effect relationship, IC50 value was 96 µg/mL. Eca-9706 cells conditioned medium could significantly increase the CAM generating total vessel area, human umbilical vein endothelial cells proliferation,migration and tube formation, while the Eca-9706 cell conditioned medium of Qige powder ethanol extract could reduce CAM angiogenesis, human umbilical vein endothelial cells proliferation and tube formation, but increase endothelial cells migration. Qige powder ethanol extract could reduce endothelial cells secreting VEGF and IL-6 while increase Eca-9706 cells secreting.
  
  
  CONCLUSIONS
  Qige powder ethanol extract can inhibit angiogenesis, endothelial cell proliferation, and tube formation induced by Eca-9706 cells, while reduce VEGF and IL-6 secretion of endothelial cells.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Yao-song
authors.initials: YS
authors.lastname: Wu
authors.forename: Su-gai
authors.initials: SG
authors.lastname: Yin
authors.forename: Hui-hui
authors.initials: HH
authors.lastname: Wang
authors.forename: Yu-long
authors.initials: YL
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement; D049109:Cell Proliferation; D002642:Chick Embryo; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004938:Esophageal Neoplasms / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D009389:Neovascularization, Pathologic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; C508600:IL6 protein, human; D015850:Interleukin-6; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A