title: Analysis of Association between Norepinephrine Transporter Gene Polymorphisms and Personality Traits of NEO-FFI in a Japanese Population.
abstract: OBJECTIVE
  Norepinephrine is an important chemical messenger that is involved in mood and stress in humans, and is reabsorbed by the norepinephrine transporter (NET). According to Cloninger's theory, the noradrenergic system mediates the personality trait of reward dependence. Thus far, although association studies on NET gene polymorphisms and Cloninger's personality traits have been reported, they yielded inconsistent results. Therefore, in the present study we investigated whether or not the 1287G/A, -182T/C and -3081A/T polymorphisms of the NET gene (SLC6A2) are associated with reward dependence-related traits, as assessed by the five-factor model.
  
  
  METHODS
  After written informed consent was obtained from participants, the three NET gene polymorphisms were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP), and personality was assessed by the Neuroticism Extraversion Openness-Five Factor Inventory (NEO-FFI) in 270 Japanese university students.
  
  
  RESULTS
  A significant relation was found between the -3081A/T functional promoter polymorphism and NEO-FFI scores: those with the T allele exhibited a lower extraversion (E) score than those without the T allele (Mann-Whitney U-test: z=-3.861, p<0.001). However, there was no correlation between the other NET gene polymorphisms and E score, and no association with other dimensions and these three polymorphisms.
  
  
  CONCLUSIONS
  We conclude that the -3081A/T functional polymorphism in the NET gene may affect the extraversion of reward dependence-related traits, as measured by NEO-FFI. However, we used only the shortened version of NEO-PI-R in this study. Further investigations are necessary using the full version of self-rating personality questionnaires.
authors.affiliation: Laboratory of Neurophysiology, Graduate School of Environmental Health Sciences, Azabu University, Kanagawa, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Narita
authors.affiliation: Laboratory of Neurophysiology, Graduate School of Environmental Health Sciences, Azabu University, Kanagawa, Japan. ; Health Administration Center, Azabu University, Kanagawa, Japan. ; Department of Neuropsychiatry, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Iwahashi
authors.affiliation: Laboratory of Neurophysiology, Graduate School of Environmental Health Sciences, Azabu University, Kanagawa, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Nagahori
authors.affiliation: Laboratory of Neurophysiology, Graduate School of Environmental Health Sciences, Azabu University, Kanagawa, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Numajiri
authors.affiliation: Laboratory of Neurophysiology, Graduate School of Environmental Health Sciences, Azabu University, Kanagawa, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Yoshihara
authors.affiliation: Laboratory of Effective Animals for Human Health, Azabu University, Kanagawa, Japan.
authors.forename: Nobuyo
authors.initials: N
authors.lastname: Ohtani
authors.affiliation: Department of Neuropsychiatry, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishigooka
keywords: Gene polymorphism; NEO-FFI; Norepinephrine transporter; Personality