title: Cryptosporidium species and subtype analysis in diarrhoeic pre-weaned lambs and goat kids from north-western Spain.
abstract: Faecal specimens from diarrhoeic pre-weaned lambs (n = 171) and goat kids (n = 118) were collected in 37 sheep and 23 goat flocks, respectively, from NW Spain and microscopically examined for the presence of Cryptosporidium oocysts. Positive specimens were selected for molecular characterization. Presence of Cryptosporidium oocysts were significantly higher in specimens from goat kids (62.7%) than from lambs (31.6%). PCR products of the SSU rRNA locus were obtained for 108 isolates, and three Cryptosporidium species were identified. Cryptosporidium parvum was the most common species identified from both lambs (74.4%) and goat kids (93.8%). The remaining PCR products from lambs (25.6%) and goat kids (7.7%) were identified as Cryptosporidium Ubiquitum and Cryptosporidium xiaoi, respectively. Five C. parvum subtypes were identified; IIaA13G1R1, IIaA14G2R1, IIaA15G2R1 and IIaA16G3R1 were found in both host species, and IIdA17G1 was only detected in goat kids. Subtype IIaA15G2R1 was the most common and widely distributed. The present study provides the first description of subtypes IIaA13G1R1 in both small ruminant species, IIaA14G2R1 in sheep and IIaA16G3R1 in goats. Our results also reveal that diarrhoeic pre-weaned lambs and goat kids must be considered important reservoirs of Cryptosporidium species with zoonotic potential, such as C. parvum and C. ubiquitum.
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain. pablo.diaz@usc.es.
authors.forename: Pablo
authors.identifier: http://orcid.org/0000-0003-2445-1095
authors.initials: P
authors.lastname: Díaz
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013, Zaragoza, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Quílez
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Prieto
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Navarro
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Pérez-Creo
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Fernández
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Panadero
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Ceferino
authors.initials: C
authors.lastname: López
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Díez-Baños
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain.
authors.forename: Patrocinio
authors.initials: P
authors.lastname: Morrondo
mesh_terms: D000818:Animals; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003967:Diarrhea / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats / Q000254:growth & development / Q000469:parasitology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000254:growth & development / Q000469:parasitology; D012757:Sheep Diseases / Q000469:parasitology*; D013030:Spain; D014886:Weaning
chemical_list: D012335:RNA, Ribosomal
keywords: C. parvum GP60 subtypes; Cryptosporidium; Goat kids; Lambs; NW Spain; SSU rRNA PCR-RFLP