title: Proteomic analysis of extracellular vesicles derived from Mycobacterium tuberculosis.
abstract: The release of extracellular vesicles, also known as outer membrane vesicles, membrane vesicles, exosomes, and microvesicles, is an evolutionarily conserved phenomenon from bacteria to eukaryotes. It has been reported that Mycobacterium tuberculosis releases extracellular vesicles harboring immunologically active molecules, and these extracellular vesicles have been suggested to be applicable in vaccine development and biomarker discovery. However, the comprehensive proteomic analysis has not been performed for M. tuberculosis extracellular vesicles. In this study, we identified a total of 287 vesicular proteins by four LC-MS/MS analyses with high confidence. In addition, we identified several vesicular proteins associated with the virulence of M. tuberculosis. This comprehensive proteome profile will help elucidate the pathogenic mechanism of M. tuberculosis. The data have been deposited to the ProteomeXchange with identifier PXD001160 (http://proteomecentral.proteomexchange.org/dataset/PXD001160).
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Jaewook
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Si-Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Dong-Sic
authors.initials: DS
authors.lastname: Choi
authors.affiliation: Section of Microbiology, International Tuberculosis Research Center, Changwon, Republic of Korea.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Dae-Kyum
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Gyeongyun
authors.initials: G
authors.lastname: Go
authors.affiliation: Pohang Center for Evaluation of Biomaterials, Pohang, Republic of Korea.
authors.forename: Seon-Min
authors.initials: SM
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea.|Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Republic of Korea.
authors.forename: Si Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea.|Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Republic of Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Pusan National University School of Medicine, Yangsan, Republic of Korea.|Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.
authors.forename: Chulhun L
authors.initials: CL
authors.lastname: Chang
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002853:Chromatography, Liquid; D000067128:Extracellular Vesicles / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000378:metabolism* / Q000472:pathogenicity; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014774:Virulence
chemical_list: D001426:Bacterial Proteins
keywords: Diagnosis; Extracellular vesicles; Microbiology; Mycobacterium tuberculosis