title: Identification of shorter length lamin A protein in mouse ear cartilage tissue.
abstract: Lamin A is an intermediate filament protein which is cleaved by the enzyme, FACE 1 at VTRSY↓L. The cleavage is the final step in the production of the mature lamin A protein. The mature lamin A protein localizes in the inner membrane of the nucleus. The mutation in the lamin A gene causes many diseases, including accelerated aging. It is known that the protein is not expressed in neuronal cells of the brain. Many splicing variants of the lamin A gene have been reported. In this study, the amino acid sequence VTRSY (a penta-peptide repeat) was found in three different sites of the C-terminal end of the lamin A protein, the protein expressed in cells of ear cartilage tissues is shorter than the protein expressed in cells of the skin tissues. Using two lamin A antibodies, it was found that the amino acid sequence between penta-peptide 2 and 3 is missing in lamin A protein that was expressed in the cells of mouse ear cartilage tissue, besides the RT-PCR data confirmed that the corresponding coding sequence between the penta repeat 2 and 3 is intact. Cleavage may occur at the penta-peptide (VTRSY) at site 3 in the lamin A tail of mouse ear cartilage.
authors.forename: Ramamoorthy M
authors.initials: RM
authors.lastname: Kalidas
authors.forename: Subramanian Elaiya
authors.initials: SE
authors.lastname: Raja
authors.forename: Sivasubramaniam
authors.initials: S
authors.lastname: Sudhakar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004425:Ear Cartilage / Q000378:metabolism*; D015870:Gene Expression; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D012867:Skin / Q000378:metabolism
chemical_list: D034904:Lamin Type A; C576142:Lmna protein, mouse