title: [Hormonal Induction of In Vitro Maturation and Ovulation of Loach Oocytes and Obtaining Egg Cells Capable of Fertilization and Development].
abstract: Loach oocytes that have reached a definitive size and are surrounded by follicular envelopes are capable of maturation and ovulating under the effect of 1 μg/mL progesterone in 75% Leibovitz medium with 1 g/L sodium bicarbonate or with pH adjustment to 9.0 by 1 N sodium hydroxide. Inseminated eggs are developed until the stage of when adding 20% bovine serum to the incubation medium. Substitution of the bovine serum with 10-20% loach ovarian fluid or 20% carp ovarian fluid provides more complete development of inseminated eggs until the stage of prolarvae that pass to active nutrition.
authors.forename: M N
authors.initials: MN
authors.lastname: Skoblina
authors.forename: A A
authors.initials: AA
authors.lastname: Minin
mesh_terms: D000818:Animals; D003531:Cypriniformes / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology*; D047108:Embryonic Development / Q000502:physiology*; D005307:Fertilization in Vitro; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010060:Ovulation