title: Synthesis of Hemoglobin Conjugated Polymeric Micelle: A ZnPc Carrier with Oxygen Self-Compensating Ability for Photodynamic Therapy.
abstract: Photodynamic therapy (PDT) is a promising singlet oxygen ((1)O2) mediated clinical treatment for many tumors. As the source of (1)O2, oxygen plays an important role in the curative effect of PDT. However, the facts of photochemical depletion of oxygen and the intrinsic hypoxic microenvironment of tumors remain the major challenges. In this work, a novel photosensitizer carrier with oxygen self-compensating ability was designed for PDT. It was synthesized via chemical conjugation of hemoglobin (Hb) to polymeric micelles formed by triblock copolymers of poly(ethylene glycol)-block-poly(acrylic acid)-block-polystyrene (PEG-b-PAA-b-PS). The PEG-b-PAA-b-PS and resultant micelles in aqueous solution were comprehensively characterized by means of FTIR, (1)H NMR, GPC, DLS, TEM, and fluorescence spectroscopy. The oxygen-binding capacity and antioxidative activity of the Hb conjugated micelles were evaluated via UV-vis spectroscopy. In addition, compared with the control micelles without Hb, the Hb conjugated photosensitizer carrier was able to generate more (1)O2 and exert greater photocytotoxicity on Hela cells in vitro.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University , Hangzhou 310027, P. R. China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University , Hangzhou 310027, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University , Suzhou, 215006, P. R. China.
authors.forename: Kui
authors.initials: K
authors.lastname: Chen
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University , Suzhou, 215006, P. R. China.
authors.forename: Gaojian
authors.initials: G
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University , Hangzhou 310027, P. R. China.
authors.forename: Kehua
authors.initials: K
authors.lastname: Tu
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University , Hangzhou 310027, P. R. China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University , Hangzhou 310027, P. R. China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Wang
mesh_terms: D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology; D054833:Isoindoles; D008823:Micelles; D010100:Oxygen / Q000737:chemistry / Q000494:pharmacology; D010778:Photochemotherapy; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D015032:Zinc / Q000737:chemistry
chemical_list: D003603:Cytotoxins; D006454:Hemoglobins; D007211:Indoles; D054833:Isoindoles; D008823:Micelles; D011092:Polyethylene Glycols; D015032:Zinc; D010100:Oxygen; C013647:phthalocyanine