title: Expression and characterization of a recombinant psychrophilic γ-carbonic anhydrase (NcoCA) identified in the genome of the Antarctic cyanobacteria belonging to the genus Nostoc.
abstract: Carbonic anhydrases (CAs, EC 4.2.1.1) catalyze the CO2 hydration/dehydration reversible reaction: CO2 + H2O ⇄ [Formula: see text] + H(+). Living organisms encode for at least six distinct genetic families of such catalyst, the α-, β-, γ-, δ-, ζ- and η-CAs. The main function of the CAs is to quickly process the CO2 derived by metabolic processes in order to regulate acid-base homeostasis, connected to the production of protons (H(+)) and bicarbonate. Few data are available in the literature on Antarctic CAs and most of the scientific information regards CAs isolated from mammals or prokaryotes (as well as other mesophilic sources). It is of great interest to study the biochemical behavior of such catalysts identified in organism living in the Antarctic sea where temperatures average -1.9 °C all year round. The enzymes isolated from Antarctic organisms represent a useful tool to study the relations among structure, stability and function of proteins in organisms adapted to living at constantly low temperatures. In the present paper, we report in detail the cloning, purification, and physico-chemical properties of NcoCA, a γ-CA isolated from the Antarctic cyanobacterium Nostoc commune. This enzyme showed a higher catalytic efficiency at lower temperatures compared to mesophilic counterparts belonging to α-, β-, γ-classes, as well as a limited stability at moderate temperatures.
authors.affiliation: a Istituto di Bioscienze e Biorisorse, CNR , Napoli , Italy .
authors.forename: Viviana
authors.initials: V
authors.lastname: De Luca
authors.affiliation: a Istituto di Bioscienze e Biorisorse, CNR , Napoli , Italy .|b Laboratorio di Chimica Bioinorganica, Dipartimento Di Chimica , Polo Scientifico, Università degli Studi di Firenze, Sesto Fiorentino , Florence , Italy .|c Dipartimento Neurofarba , Sezione di Scienze Farmaceutiche, Polo Scientifico, Sesto Fiorentino , Florence , Italy , and.
authors.forename: Sonia
authors.initials: S
authors.lastname: Del Prete
authors.affiliation: b Laboratorio di Chimica Bioinorganica, Dipartimento Di Chimica , Polo Scientifico, Università degli Studi di Firenze, Sesto Fiorentino , Florence , Italy .|c Dipartimento Neurofarba , Sezione di Scienze Farmaceutiche, Polo Scientifico, Sesto Fiorentino , Florence , Italy , and.
authors.forename: Daniela
authors.initials: D
authors.lastname: Vullo
authors.affiliation: a Istituto di Bioscienze e Biorisorse, CNR , Napoli , Italy .
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Carginale
authors.affiliation: a Istituto di Bioscienze e Biorisorse, CNR , Napoli , Italy .
authors.forename: Pietro
authors.initials: P
authors.lastname: Di Fonzo
authors.affiliation: d Department of Chemistry , College of Science, King Saud University , Riyadh , Saudi Arabia.
authors.forename: Sameh M
authors.initials: SM
authors.lastname: Osman
authors.affiliation: d Department of Chemistry , College of Science, King Saud University , Riyadh , Saudi Arabia.
authors.forename: Zeid
authors.initials: Z
authors.lastname: AlOthman
authors.affiliation: b Laboratorio di Chimica Bioinorganica, Dipartimento Di Chimica , Polo Scientifico, Università degli Studi di Firenze, Sesto Fiorentino , Florence , Italy .|c Dipartimento Neurofarba , Sezione di Scienze Farmaceutiche, Polo Scientifico, Sesto Fiorentino , Florence , Italy , and.|d Department of Chemistry , College of Science, King Saud University , Riyadh , Saudi Arabia.
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
authors.affiliation: a Istituto di Bioscienze e Biorisorse, CNR , Napoli , Italy .
authors.forename: Clemente
authors.initials: C
authors.lastname: Capasso
mesh_terms: D000595:Amino Acid Sequence; D000864:Antarctic Regions; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D016680:Genome, Bacterial / Q000235:genetics*; D046937:Nostoc / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011994:Recombinant Proteins; D002256:Carbonic Anhydrases
keywords: Antarctic carbonic anhydrase; carbonic anhydrase; cyanobacteria; hydratase activity; metalloenzymes