title: Modulation of neuronal network activity using magnetic nanoparticle-based astrocytic network integration.
abstract: Investigating the mechanisms of the neuron-glia interaction is important in the basic research of neuroscience and neural transplantation. Synaptic transmission is modulated by astrocyte activation in the pre- and post-synaptic terminals, and this phenomenon is spread to the surrounding astrocytes through gap junctions. However, the modulation of network-wide neuronal activity dependent on extensive astrocyte activation is not well understood. In this study, we show network-wide neuronal modulation associated with a newly developed three-dimensional neuronal and astrocytic network co-culture method. To establish widespread neuronal and astrocytic network interactions in vitro, we performed integration of magnetic nanoparticle-injected astrocytes (Mag-AS) onto the matured monolayer of neuronal networks using an external magnetic force. The neuronal electrical activity was dynamically synchronized at 24 h after integration of the Mag-AS network. In addition, Mag-AS network activation using a caged calcium compound rapidly induced suppression and subsequent synchronization of neuronal electrical activity. These results indicate that the high-density astrocytic network integration onto the neuronal network can induce widespread neuronal modulation, and our in vitro co-culture method contributes to the advancement of neuronal and astrocytic transplantation research.
authors.affiliation: Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI and ), Japan. saito@criepi.denken.or.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimba
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Takayama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kotani
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Jimbo
mesh_terms: D001253:Astrocytes / Q000737:chemistry* / Q000166:cytology; D017610:Calcium Compounds / Q000737:chemistry*; D017629:Gap Junctions / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D009435:Synaptic Transmission
chemical_list: D017610:Calcium Compounds; D058185:Magnetite Nanoparticles