title: Calcium Spikes in Epithelium: study on Drosophila early embryos.
abstract: Calcium ion acts in nearly every aspect of cellular life. The versatility and specificity required for such a ubiquitous role is ensured by the spatio-temporal dynamics of calcium concentration variations. While calcium signal dynamics has been extensively studied in cell cultures and adult tissues, little is known about calcium activity during early tissue morphogenesis. We monitored intracellular calcium concentration in Drosophila gastrula and revealed single cell calcium spikes that were short-lived, rare and showed strong variability among embryos. We quantitatively described the spatio-temporal dynamics of these spikes and analyzed their potential origins and nature by introducing physical and chemical perturbations. Our data highlight the inter- and intra-tissue variability of calcium activity during tissue morphogenesis.
authors.affiliation: Aix Marseille Université, CNRS, IBDM UMR 7288, 13288, Marseille, France.
authors.forename: Olga
authors.initials: O
authors.lastname: Markova
authors.affiliation: Aix Marseille Université, CNRS, IBDM UMR 7288, 13288, Marseille, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Sénatore
authors.affiliation: Aix Marseille Université, CNRS, IBDM UMR 7288, 13288, Marseille, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Chardès
authors.affiliation: Aix Marseille Université, CNRS, IBDM UMR 7288, 13288, Marseille, France.
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Lenne
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology*; D004848:Epithelium / Q000378:metabolism
chemical_list: D002118:Calcium