title: Effect of GBOT on blood lipid and blood glucose metabolism in rats with atherosclerosis.
abstract: We observed the variation in in vivo blood lipid and blood glucose metabolism in rats with atherosclerosis after 5-(3,4-dihydroxy-phenyl)-1-piperidin-1-yl-penta-2,4-dien-1-one (GBOT) administration. Wistar rats aged 10 weeks received a high-fat diet to establish the atherosclerosis model. Metabolic indices related to blood lipid and blood glucose were measured before modeling and at 4 and 8 weeks after modeling. Liver fat levels in rats were measured at 8 weeks to analyze the relationship between liver fat and blood lipid levels. We examined the mechanism of blood lipid reduction. The levels of serum triglycerides, total cholesterol, and very-low-density lipoprotein cholesterol in rats in the control group were significantly decreased (P < 0.05) compared with those in the 4-week control group at 4 weeks and decreased significantly and continuously until the 8th week (P < 0.05). Compared with the 8-week control group, the blood glucose level in rats in the 8-week experimental group decreased significantly (P < 0.05), and the level of insulin sensitivity index decreased significantly (P < 0.05). Compared with the control group, triglyceride and total cholesterol levels per unit mass in rat liver tissue in the 8-week experimental group decreased significantly (P < 0.05). Western blotting indicated that GBOT significantly increased the expression of lecithin-cholesterol acyltransferase, low-density lipoprotein receptor, and cholesterol 7 alpha-hydroxylase proteins. GBOT can significantly decrease the levels of blood lipid and blood glucose in rat models of atherosclerosis, and its mechanism may be associated with the promotion of expression of lecithin-cholesterol acyltransferase, low-density lipoprotein receptor, and cholesterol 7 alpha-hydroxylase proteins.
authors.affiliation: School of Chinese Material Medical, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: A
authors.initials: A
authors.lastname: Rong
authors.affiliation: Institute of Mongolian Medicine Chemistry, Inner Mongolia University, Hohhot, China.
authors.forename: G
authors.initials: G
authors.lastname: Borjihan
authors.affiliation: School of Chinese Material Medical, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Qiao
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000473:pathology; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood* / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism*; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism; D010880:Piperidines / Q000737:chemistry / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000737:chemistry / Q000494:pharmacology*; D017208:Rats, Wistar; D011973:Receptors, LDL / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: C000607169:5-(3,4-dihydroxyphenyl)-1-piperidin-1-yl-penta-2,4-dien-1-one; D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D011973:Receptors, LDL; D014280:Triglycerides; D002784:Cholesterol; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C029009:steroid hormone 7-alpha-hydroxylase; D007862:Phosphatidylcholine-Sterol O-Acyltransferase