title: Quantification of proteome changes in bovine muscle from two-dimensional electrophoresis data.
abstract: Proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress were assessed on the basis of two-dimensional electrophoresis (2-DE) data. In this study, the bootstrap resampling statistical technique and a new measure of relative change of the volume of 2-DE protein spots are shown to be more efficient than commonly used statistics to reliably quantify changes in protein abundance in stress response. The data are supplied in this article and are related to "Tackling proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress" by Franco et al. [1].
authors.affiliation: Meat Technology Center of Galicia, r/Galicia 4, Parque Tecnolóxico de Galicia, San Cibrao das Viñas, Ourense 32900, Galicia, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Franco
authors.affiliation: Department of Genetics, University of Santiago de Compostela, Santiago de Compostela 15782, Spain.
authors.forename: Ariadna
authors.initials: A
authors.lastname: Mato
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, Santiago de Compostela 15782, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Salgado
authors.affiliation: Department of Genetics, University of Santiago de Compostela, Santiago de Compostela 15782, Spain.
authors.forename: María
authors.initials: M
authors.lastname: López-Pedrouso
authors.affiliation: Spanish National Research Council (CSIC), Marine Research Institute (IIM), Eduardo Cabello 6, Vigo 36208, Pontevedra, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Carrera
authors.affiliation: Proteomics Laboratory, CHUS, Santiago de Compostela 15782, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Bravo
authors.affiliation: Department of Genetics, University of Santiago de Compostela, Santiago de Compostela 15782, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Parrado
authors.affiliation: Spanish National Research Council (CSIC), Marine Research Institute (IIM), Eduardo Cabello 6, Vigo 36208, Pontevedra, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Gallardo
authors.affiliation: Department of Genetics, University of Santiago de Compostela, Santiago de Compostela 15782, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Zapata
keywords: Bootstrap resampling method; Bovine muscle proteome; Quantitative proteomics; Relative and fold change measures; Stress-dependent proteome changes; Two-dimensional electrophoresis