title: Association of Anti-Emetic Efficacy of Ondansetron with G2677T Polymorphism in a Drug Transporter Gene ABCB1 in Pakistani Population.
abstract: OBJECTIVE
  To determine the association of ABCB1 polymorphism G2677T with anti-emetic efficacy in patients treated with ondansetron for preventing postoperative nausea and vomiting.
  
  
  METHODS
  A clinical trial.
  
  
  METHODS
  Combined Military Hospital, Rawalpindi and Institute of Biomedical and Genetic Engineering, Islamabad, from 2012 to 2013.
  
  
  METHODS
  Four mg ondansetron was administered intravenously 30 minutes before the end of surgery. A total of 246 patients with the complaints of nausea and vomiting and 244 patients without nausea and vomiting were analyzed for G2677T polymorphism using PCR-RFLP method. Results were described as frequency percentages and chi-square test with significance at p < 0.05.
  
  
  RESULTS
  The patients with TT genotype had significantly lower incidence of postoperative nausea and vomiting during the first 2 hours (p < 0.001) and between 2 - 24 hours after surgery as compared to other genotypes (p < 0.001). The patients with GG genotypes had significantly higher incidence of this complaint (p=0.014).
  
  
  CONCLUSIONS
  Polymorphism of ABCB1 has an association with responsiveness for ondansetron. There is a role for genetics in the management of PONV.
authors.affiliation: Department of Pharmacology and Therapeutics, Army Medical College, Rawalpindi.
authors.forename: Kulsoom
authors.initials: K
authors.lastname: Farhat
authors.affiliation: Department of Pharmacy, Baluchistan University, Pakistan.
authors.forename: Javeid
authors.initials: J
authors.lastname: Iqbal
authors.affiliation: Department of Pharmacology and Therapeutics, Army Medical College, Rawalpindi.
authors.forename: Akbar
authors.initials: A
authors.lastname: Waheed
authors.affiliation: Department of Genetics, Institute of Biomedical and Genetic Engineering, Islamabad.
authors.forename: Qaisar
authors.initials: Q
authors.lastname: Mansoor
authors.affiliation: Department of Genetics, Institute of Biomedical and Genetic Engineering, Islamabad.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ismail
authors.affiliation: Department of Anesthesia, Combined Military Hospital, Rawalpindi.
authors.forename: Anwar Kamal
authors.initials: AK
authors.lastname: Pasha
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics*; D061605:Administration, Intravenous; D000293:Adolescent; D000328:Adult; D000368:Aged; D000932:Antiemetics / Q000008:administration & dosage* / Q000627:therapeutic use; D044466:Asian People / Q000235:genetics; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D017294:Ondansetron / Q000008:administration & dosage* / Q000627:therapeutic use; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020250:Postoperative Nausea and Vomiting / Q000208:ethnology / Q000235:genetics / Q000517:prevention & control*; D011292:Premedication / Q000706:statistics & numerical data; D016896:Treatment Outcome
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D000932:Antiemetics; D017294:Ondansetron