title: MicroRNA-708 is downregulated in hepatocellular carcinoma and suppresses tumor invasion and migration.
abstract: BACKGROUND
  MicroRNA-708 (miR-708) has been identified as one of down-regulated miRNAs in hepatocellular carcinoma (HCC) tissues by Taqman miRNAs array and confirmed quantitatively by reverse transcription polymerase chain reaction (qRT-PCR). However, its involvement in HCC remains unclear. The aim of this study was to investigate the roles of miR-708 in carcinogenesis and cancer progression of HCC.
  
  
  METHODS
  QRT-PCR was performed to detect the expression of miR-708 in 100 pairs of HCC and adjacent non cancerous tissues. Then, its associations with various clinicopathological features of HCC patients were statistically evaluated. After that, we also observed the effects of enforced miR-708 expression on migration and invasion of HCC cells in vitro.
  
  
  RESULTS
  Our data confirmed that the expression level of miR-708 in HCC tissues was significantly lower than those in adjacent non-cancerous tissues (P=0.001). In addition, low miR-708 expression was found to be closely correlated with high Edmondson-Steiner grading (P=0.02) and advanced Tumor Node Metastasis (TNM) stage (P=0.01). Furthermore, the enforced expression of miR-708 could suppress the migration and invasion of HCC cell lines in vitro.
  
  
  CONCLUSIONS
  Our findings support that miR-708, which is frequently down-regulated in HCC, may contribute to the aggressive progression of HCC and inhibit HCC cell mobility. Further studies on the identification of its target genes are required to be performed.
authors.affiliation: The First Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China.
authors.forename: Guangjun
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China. Electronic address: fangy_xxyfy@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: The First Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xu
authors.affiliation: The First Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Yue
authors.affiliation: The Second Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China.
authors.forename: Xiangjie
authors.initials: X
authors.lastname: Fang
authors.affiliation: The Second Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000502:physiology*; D015331:Cohort Studies; D015536:Down-Regulation / Q000502:physiology*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology
chemical_list: C568528:MIRN708 microRNA, human; D035683:MicroRNAs
keywords: Cancer progression; Hepatocellular carcinoma; Invasion; Migration; microRNA-708