title: Chemometrics-assisted determination of amiloride and triamterene in biological fluids with overlapped peaks and unknown interferences.
abstract: BACKGROUND
  Amiloride (AMI) and triamterene (TRI) are both potassium-saving diuretics, which are ordinarily used as doping to enhance the performance of athletes in sports. For the similar structures and complex matrices existence, chromatography and extraction are commonly employed to realize the determination of AMI and TRI in biological fluids, which are very time-consuming and laborious.
  
  
  RESULTS
  A novel method is presented to simultaneous interference-free determination of AMI and TRI in complex biological fluids samples using excitation-emission matrix fluorescence coupled with second-order calibration method based on alternating normalization-weight error algorithm.
  
  
  CONCLUSIONS
  The proposed method can obtain accurate qualitative and quantitative information of the analytes, even in the presence of the interference from complex biological fluids, which requires few prior purification and separation procedures.
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Shou-Xia
authors.initials: SX
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D000584:Amiloride / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D011786:Quality Control; D014223:Triamterene / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000584:Amiloride; D014223:Triamterene