title: A cross-priming amplification assay coupled with vertical flow visualization for detection of Vibrio parahaemolyticus.
abstract: Vibrio parahaemolyticus is a marine seafood-borne pathogen that causes gastrointestinal disorders in humans. In this study, we developed a cross-priming amplification (CPA) assay coupled with vertical flow (VF) visualization for rapid and sensitive detection of V. parahaemolyticus. This assay correctly detected all target strains (n = 13) and none of the non-target strains (n = 27). Small concentrations of V. parahaemolyticus (1.8 CFU/mL for pure cultures and 18 CFU/g for reconstituted samples) were detected within 1 h. CPA-VF can be applied at a large scale and can be used to detect V. parahaemolyticus strains rapidly in seafood and environmental samples, being especially useful in the field.
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China. Electronic address: xds666092@126.com.
authors.forename: Deshun
authors.initials: D
authors.lastname: Xu
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
authors.forename: Jiankang
authors.initials: J
authors.lastname: Han
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ji
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Shen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004783:Environmental Microbiology; D005767:Gastrointestinal Diseases / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014735:Vibrio Infections / Q000175:diagnosis; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins
keywords: Cross-priming amplification; Rapid detection; Seafood; Vertical flow visualization; Vibrio parahaemolyticus