title: Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line.
abstract: BACKGROUND
  Reactive Oxygen Species (ROS) impair the physiological functions of Retinal Pigment Epithelial (RPE) cells, which are known as one major cause of age-related macular degeneration and retinopathy diseases. The purpose of this study is to explore the cytoprotective effects of the antioxidant Bucida buceras extract in co-treatment with hydrogen peroxide (H2O2) delivery as a single addition or with continuous generation using glucose oxidase (GOx) in ARPE-19 cell cultures. The mechanism of Bucida buceras extract is believed to be associated with their antioxidant capacity to protect cells against oxidative stress.
  
  
  METHODS
  A comparative oxidative stress H2O2-induced was performed by addition and enzymatic generation using glucose oxidase on human retinal pigment epithelial cells line. H2O2-induced injury was measured by toxic effects (cell death and apoptotic pathway) and intracellular redox status: glutathione (GSH), antioxidant enzymes (catalase and glutathione peroxidase) and reducing power (FRAP). The retino-protective effect of co-treatment with Bucida buceras extract on H2O2-induced human RPE cell injury was investigated by cell death (MTT assay) and oxidative stress biomarkers (H2O2, GSH, CAT, GPx and FRAP).
  
  
  RESULTS
  Bucida buceras L. extract is believed to be associated with the ability to prevent cellular oxidative stress. When added as a pulse, H2O2 is rapidly depleted and the cytotoxicity analyses show that cells can tolerate short exposure to high peroxide doses delivered as a pulse but are susceptible to lower chronic doses. Co-treatment with Bucida buceras was able to protect the cells against H2O2-induced injury. In addition to preventing cell death treatment with antioxidant plant could also reverse the significant decrease in GSH level, catalase activity and reducing power caused by H2O2.
  
  
  CONCLUSIONS
  These findings suggest that Bucida buceras could protect RPE against ocular pathogenesis associated with oxidative stress induced by H2O2-delivered by addition and enzymatic generation.
authors.affiliation: Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. ilokiasb@yahoo.com.
authors.forename: Simon Bernard
authors.initials: SB
authors.lastname: Iloki-Assanga
authors.affiliation: Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. lidianyslewis@rubiopharma.com.
authors.forename: Lidianys María
authors.initials: LM
authors.lastname: Lewis-Luján
authors.affiliation: Universidad de Sonora (UNISON) Hermosillo, Blvd. Luis Encinas y Rosales S/N Col. Centro, Hermosillo, Sonora, C.P. 83210, Mexico. dfernandez@rubiopharma.com.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fernández-Angulo
authors.affiliation: Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. andreagil@libaf.com.
authors.forename: Armida Andrea
authors.initials: AA
authors.lastname: Gil-Salido
authors.affiliation: Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. claudialara@libaf.com.
authors.forename: Claudia Lizeth
authors.initials: CL
authors.lastname: Lara-Espinoza
authors.affiliation: Rubio Pharma y Asociados S. A de C. V., Laboratorio de Investigaciones en Bioactivos y Alimentos Funcionales (LIBAF), Blvd. García Morales Km. 6.5 # 330. El Llano. Hermosillo, Sonora, C.P. 83210, Mexico. medicinabiologica@rubiopharma.com.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Rubio-Pino
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D029764:Combretaceae / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D055213:Retinal Pigment Epithelium / Q000166:cytology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D010088:Oxidoreductases