title: Large two-centre study into the prevalence of Mycoplasma genitalium and Trichomonas vaginalis in the Netherlands.
abstract: Mycoplasma genitalium and Trichomonas vaginalis are common sexually transmitted infections (STIs). In the Netherlands, testing for M. genitalium and T. vaginalis is not recommended for first-line STI screening. Recent reports about the increasing antimicrobial resistance in M. genitalium raise concern about the adequacy of current empirical treatment regimens. It is necessary to have insight in the prevalence of M. genitalium and T. vaginalis in order to evaluate current first-line STI screening and treatment protocols. During a five-month period, samples sent to two large medical microbiology diagnostic centres in the Netherlands for STI screening (Chlamydia trachomatis and Neisseria gonorrhoeae) were retrospectively tested for the prevalence of M. genitalium and T. vaginalis using the Diagenode S-DiaMGTV kit. A total of 1569 samples from 1188 unique patients (55.4% female) were tested. M. genitalium was the second most prevalent STI detected (4.5% of the patients), after C. trachomatis (8.3%). T. vaginalis was detected in 1.4% of the patients, comparable to the prevalence of N. gonorrhoeae (1.3%). Dual infections were only detected in a small number of patients (1.0%). Incorporation of M. genitalium into routine STI screening should be considered, because of its relatively high prevalence, the consequences of its detection for antibiotic treatment and because of the availability of easy-to-use molecular diagnostic tests. For T. vaginalis, routine screening may be considered, depending on local prevalence and (sub)population.
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands arjan.dejong@radboudumc.nl.
authors.forename: A S
authors.initials: AS
authors.lastname: de Jong
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: J C
authors.initials: JC
authors.lastname: Rahamat-Langendoen
authors.affiliation: PAMM, Laboratory of Medical Microbiology, Veldhoven, the Netherlands.
authors.forename: Ptw
authors.initials: P
authors.lastname: van Alphen
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Hilt
authors.affiliation: PAMM, Laboratory of Medical Microbiology, Veldhoven, the Netherlands.
authors.forename: Cmc
authors.initials: C
authors.lastname: van Herk
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: Sbeh
authors.initials: S
authors.lastname: Pont
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: Wjg
authors.initials: W
authors.lastname: Melchers
authors.affiliation: PAMM, Laboratory of Medical Microbiology, Veldhoven, the Netherlands.
authors.forename: Jhb
authors.initials: J
authors.lastname: van de Bovenkamp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D006801:Humans; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D045704:Mycoplasma genitalium / Q000302:isolation & purification*; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D009426:Netherlands / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D015995:Prevalence; D012189:Retrospective Studies; D012749:Sexually Transmitted Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology; D014247:Trichomonas Vaginitis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000302:isolation & purification*; D055815:Young Adult
keywords: Mycoplasma genitalium; Protozoal disease; Screening; Trichomoniasis (Trichomonas vaginalis); sexually transmitted infection