title: Field populations of native Indian honey bees from pesticide intensive agricultural landscape show signs of impaired olfaction.
abstract: Little information is available regarding the adverse effects of pesticides on natural honey bee populations. This study highlights the detrimental effects of pesticides on honey bee olfaction through behavioural studies, scanning electron microscopic imaging of antennal sensillae and confocal microscopic studies of honey bee brains for calcium ions on Apis cerana, a native Indian honey bee species. There was a significant decrease in proboscis extension response and biologically active free calcium ions and adverse changes in antennal sensillae in pesticide exposed field honey bee populations compared to morphometrically similar honey bees sampled from low/no pesticide sites. Controlled laboratory experiments corroborated these findings. This study reports for the first time the changes in antennal sensillae, expression of Calpain 1(an important calcium binding protein) and resting state free calcium in brains of honey bees exposed to pesticide stress.
authors.affiliation: 1] Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India [2] Centre for Pollination Studies, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India.
authors.forename: Priyadarshini
authors.initials: P
authors.lastname: Chakrabarti
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Rana
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India.
authors.forename: Sreejata
authors.initials: S
authors.lastname: Bandopadhyay
authors.affiliation: Agharkar Research Institute, G G Agarkar Road, Pune - 411004, India.
authors.forename: Dattatraya G
authors.initials: DG
authors.lastname: Naik
authors.affiliation: Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India.
authors.forename: Sagartirtha
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: 1] Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India [2] Centre for Pollination Studies, University of Calcutta, 35 Ballygunge Circular Road, Kolkata - 700019, India.
authors.forename: Parthiba
authors.initials: P
authors.lastname: Basu
mesh_terms: D000818:Animals; D001516:Bees / Q000187:drug effects* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure / Q000009:adverse effects*; D007194:India; D010573:Pesticide Residues / Q000506:poisoning*; D012903:Smell / Q000187:drug effects* / Q000502:physiology*; D062211:Spatio-Temporal Analysis
chemical_list: D010573:Pesticide Residues