title: [Effect of Tiam 1 on cell proliferation and migration in head and neck squamous cell carcinoma cells].
abstract: OBJECTIVE
  To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells.
  
  
  METHODS
  Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay.
  
  
  RESULTS
  Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05).
  
  
  CONCLUSIONS
  Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
authors.forename: Ping
authors.initials: P
authors.lastname: Xiao
authors.forename: Quiyang
authors.initials: Q
authors.lastname: Fu
authors.forename: Zeyong
authors.initials: Z
authors.lastname: Li
authors.forename: Xuehui
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Fu
authors.forename: Renqiang
authors.initials: R
authors.lastname: Wan
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D000077195:Squamous Cell Carcinoma of Head and Neck; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; D014162:Transfection
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human