title: Emergence of Acinetobacter pittii harboring New Delhi metallo-beta-lactamase genes in Daejeon, Korea.
abstract: Carbapenemase production has been reported worldwide in gram-negative bacteria, including Acinetobacter species. We detected carbapenemase-producing Acinetobacter pittii in clinical isolates in Daejeon, Korea. Twenty-one ertapenem-resistant A. pittii isolates screened with a disk diffusion method were characterized by using the Epsilon test, four multiplex PCR assays, and a multilocus sequence typing (MLST) scheme. A total of 21 A. pittii isolates harbored the metallo-β-lactamase (MBL) gene bla(IMP-1) or bla(NDM-1). Nineteen isolates containing bla(IMP-1) were resistant to imipenem and meropenem, but two isolates harboring bla(NDM-1) were susceptible to them. The sequence types (STs) of the two New Delhi MBL (NDM-1)-producing A. pittii isolates were ST70 and ST207, which differed from the STs (ST63, ST119, ST396, and a novel ST) of the IMP-1-producing A. pittii. This is the first report on NDM-1-producing A. pittii isolates in Korea. Our results emphasize that the study of NDM-1-producing gram-negative bacteria should involve carbapenem-susceptible as well as carbapenem-resistant isolates.
authors.affiliation: Department of Biomedical Laboratory Science, Far East University, Eumseong-gun, Chungcheongbuk-do, Korea.
authors.forename: Ji Youn
authors.initials: JY
authors.lastname: Sung
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon, Korea. shkoo@cnu.ac.kr.
authors.forename: Sun Hoe
authors.initials: SH
authors.lastname: Koo
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Semi
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Gye Cheol
authors.initials: GC
authors.lastname: Kwon
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D000077731:Meropenem; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D056910:Republic of Korea; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D013845:Thienamycins; D015378:Imipenem; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; D000077731:Meropenem
keywords: A. pittii; Carbapenem; Metallo-β-lactamase; NDM-1