title: [Effect of indomethacin on BCR/ABL-Wnt/β-catenin pathway in K562 cells].
abstract: OBJECTIVE
  To investigate the effect of indomethacin on the proliferation and Wnt/β-catenin pathway in K562 cells.
  
  
  METHODS
  The cell growth of K562 cells treated with different concentrations of indomethacin was assessed with MTT assay, and the colony-forming ability of the cells was evaluated by colony-forming assay. The mRNA expressions of BCR/ABL and β-catenin were detected by RT-PCR, and the protein expressions of pBCR/ABL, total BCR/ABL, β-catenin, pGSK-3β and c-myc were analyzed by Western blotting.
  
  
  RESULTS
  Indomethacin significantly suppressed the growth and colony-forming ability of K562 cells in a dose-dependent manner. Indomethacin treatment dose-dependently decreased the protein level of pBCR/ABL and total BCR/ABL without affecting bcr-abl mRNA expressions. Compared with the control groups, indomethacin-treated cells showed obviously decreased mRNA and protein expressions of β-catenin and decreased protein expressions of pGSK-3β and c-myc.
  
  
  CONCLUSIONS
  Indomethacin inhibits the proliferation of K562 cells by suppressing the activity of bcr-abl-Wnt/β-catenin pathway.
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.E-mail: liuling20071@163.com.
authors.forename: Zhangling
authors.initials: Z
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Zhenglan
authors.initials: Z
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Wenli
authors.initials: W
authors.lastname: Feng
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D020014:K562 Cells / Q000187:drug effects; D012333:RNA, Messenger; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D012333:RNA, Messenger; D051176:beta Catenin; D016044:Fusion Proteins, bcr-abl; D007213:Indomethacin