title: Effect of hyaluronic acids as chondroprotective in experimental model of osteoarthrosis.
abstract: OBJECTIVE
  to analyze the effects of hyaluronic acid of different molecular weights in an experimental model of osteoarthritis in rabbits.
  
  
  METHODS
  forty-four male California rabbits were divided randomly into three groups and underwent resection of the anterior cruciate ligament in his right knee. After three weeks of the surgical procedure began three weekly intra-articular injections of hyaluronic acid native (Polireumin(®))-PR, hyaluronic acid branched chain (Synvisc(®))-S and 0.9% saline-P. All animals were sacrificed after twelve weeks of surgery and tibial plateau infiltrated the knees were dissected. Histological cartilage of the support areas of the tibial plateaus were stained with Alcian Blue pH 1.0, Alcian Blue pH = 2.5 and toluidine blue for research on the amount of proteoglycans. The intensity of staining was quantified on a Zeiss microscope apparatus Imager Z2 MetaSystems and analyzed by software MetaferMsearch.
  
  
  RESULTS
  the effect of chondroprotetor hyaluronic acids used in the study was confirmed when compared to the control group, but the comparison made between them, there was no statistically significant difference regarding chondroprotetion.
  
  
  CONCLUSIONS
  the hyaluronic acids tested had chondroprotective effect, with no statistical difference with regard to the different molecular weights.
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Marcello Zaia
authors.initials: MZ
authors.lastname: Oliveira
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Mauro Batista
authors.initials: MB
authors.lastname: Albano
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Mario Massatomo
authors.initials: MM
authors.lastname: Namba
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Luiz Antônio Munhoz
authors.initials: LA
authors.lastname: da Cunha
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Renan Rodrigues
authors.initials: RR
authors.lastname: de Lima Gonçalves
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Edvaldo Silva
authors.initials: ES
authors.lastname: Trindade
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Lucas Ferrari
authors.initials: LF
authors.lastname: Andrade
authors.affiliation: Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Leandro
authors.initials: L
authors.lastname: Vidigal
keywords: Anterior cruciate ligament; Hyaluronic acid; Knee; Osteoarthritis; Rabbits