title: Cardiogenic differentiation of mesenchymal stem cells with gold nanoparticle loaded functionalized nanofibers.
abstract: Cardiac tissue engineering promises to revolutionize the treatment of patients with end-stage heart failure and provide new solutions to the serious problems of shortage of heart donors. The influence of extracellular matrix (ECM) plays an influential role along with nanostructured components for guided stem cell differentiation. Hence, nanoparticle embedded Nanofibrous scaffolds of FDA approved polycaprolactone (PCL), Vitamin B12 (Vit B12), Aloe Vera(AV) and Silk fibroin(SF) was constructed to differentiate mesenchymal stem cells into cardiac lineage. Cardiomyocytes (CM) and Mesenchymal stem cells (MSC) were co-cultured on these fabricated nanofibrous scaffolds for the regeneration of infarcted myocardium. Results demonstrated that synthesized gold nanoparticles were of the size 16 nm and the nanoparticle loaded nanofibrous scaffold has a mechanical strength of 2.56 MPa matching that of the native myocardium. The gold nanoparticle blended PCL scaffolds were found to be enhancing the MSCs proliferation and differentiation into cardiogenesis. Most importantly the phenotype and cardiac marker expression in differentiated MSCs were highly resonated in gold nanoparticle loaded nanofibrous scaffolds. The appropriate mechanical strength provided by the functionalized nanofibrous scaffolds profoundly supported MSCs to produce contractile proteins and achieve typical cardiac phenotype.
authors.affiliation: Center for Nanofibers & Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore; Department of Genetic Engineering, SRM University, Kattankulathur, Chennai, India.
authors.forename: Sreepathy
authors.initials: S
authors.lastname: Sridhar
authors.affiliation: Center for Nanofibers & Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore. Electronic address: nnijrv@nus.edu.sg.
authors.forename: Jayarama Reddy
authors.initials: JR
authors.lastname: Venugopal
authors.affiliation: Center for Nanofibers & Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Sridhar
authors.affiliation: Center for Nanofibers & Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D018920:Coculture Techniques; D006046:Gold / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D032383:Myocytes, Cardiac / Q000166:cytology*; D057139:Nanofibers; D011817:Rabbits
chemical_list: D006046:Gold
keywords: Electrospinning; Gold nanoparticles; Mesenchymal stem cells; Nanofibrous scaffolds; PCL; Vitamin B12