title: Galectin-1 reduced the effect of LPS on the IL-6 production in decidual cells by inhibiting LPS on the stimulation of IκBζ.
abstract: Pregnancy is a complex process where several physiological pathways interact. The down-regulated inflammatory response and the abundance of anti-inflammatory molecules during gestation may explain the acceptance of the fetus and the lack of immune response against it, even though it is a foreign tissue for the mother. NF-κB is a key regulator of the transcription of inflammatory genes, such as IL-8, IL-1β, TNF-α, or IL-6. Increased NF-κB activity that leads to the production of proinflammatory cytokines may induce obstetric disorders, such as preterm birth or abortion. Low activity of this transcription factor is associated with the beneficial anti-inflammatory environment during fetus development until delivery. Galectin-1 (Gal-1) is a lectin-type glycan-binding protein that is able to down-regulate inflammation. It has been shown that Gal-1 is abundantly expressed at the feto-maternal interface in humans, where it promotes maternal immune tolerance to the fetal semi-allograft. Gal-1 tolerance-promoting mechanisms have been established for adaptive immune cells, such as T cells and dendritic cells. However, the role of this lectin has not been established in non-immune cells at the feto-maternal interface. Here, we determined that Gal-1 is able to block the stimulating effect of LPS on IL-6 in human decidua cells. Our results show that Gal-1 acts by inhibiting the stimulation of the LPS-induced IκBζ expression, an NF-κB regulator involved in IL-6 gene transcription.
authors.affiliation: Department of Immunology of the National School of Biological Sciences of the National Polytechnic Institute, Carpio y Plan de Ayala s/n. Col. Santo Tomas, Del. Miguel Hidalgo, C.P. 11340 Mexico City, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gómez-Chávez
authors.affiliation: Department of Immunobiochemistry, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico.
authors.forename: Violeta
authors.initials: V
authors.lastname: Castro-Leyva
authors.affiliation: Department of Immunobiochemistry, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico.
authors.forename: Aurora
authors.initials: A
authors.lastname: Espejel-Núñez
authors.affiliation: Unit of Medical Research in Human Genetics, National Medical Center, IMSS, CMN siglo XXI, Mexico City, Mexico.
authors.forename: Rubén G
authors.initials: RG
authors.lastname: Zamora-Mendoza
authors.affiliation: Unit of Medical Research in Human Genetics, National Medical Center, IMSS, CMN siglo XXI, Mexico City, Mexico.
authors.forename: Haydee
authors.initials: H
authors.lastname: Rosas-Vargas
authors.affiliation: Department of Microbiology of the National School of Biological Sciences of the National Polytechnic Institute, Carpio y Plan de Ayala s/n. Col. Santo Tomas, Del. Miguel Hidalgo, C.P. 11340 Mexico City, Mexico.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Cancino-Díaz
authors.affiliation: Department of Immunology of the National School of Biological Sciences of the National Polytechnic Institute, Carpio y Plan de Ayala s/n. Col. Santo Tomas, Del. Miguel Hidalgo, C.P. 11340 Mexico City, Mexico.
authors.forename: Mario E
authors.initials: ME
authors.lastname: Cancino-Díaz
authors.affiliation: Department of Immunobiochemistry, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Estrada-Gutierrez
authors.affiliation: Department of Immunology of the National School of Biological Sciences of the National Polytechnic Institute, Carpio y Plan de Ayala s/n. Col. Santo Tomas, Del. Miguel Hidalgo, C.P. 11340 Mexico City, Mexico. Electronic address: sandrarodm@yahoo.com.mx.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rodríguez-Martínez
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003656:Decidua / Q000276:immunology*; D005260:Female; D037483:Galectin 1 / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D020672:I-kappa B Proteins / Q000276:immunology*; D015850:Interleukin-6 / Q000276:immunology*; D008070:Lipopolysaccharides / Q000633:toxicity*; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000276:immunology
chemical_list: D037483:Galectin 1; D020672:I-kappa B Proteins; C508600:IL6 protein, human; D015850:Interleukin-6; C445499:LGALS1 protein, human; D008070:Lipopolysaccharides; C105627:NFKBIE protein, human; D011518:Proto-Oncogene Proteins
keywords: Galectin-1; IL-6; IκBζ; NF-κB; Pregnancy