title: Composition and variation analysis of the TCR β-chain CDR3 repertoire in systemic lupus erythematosus using high-throughput sequencing.
abstract: The ability of T lymphocytes to mount an immune response against a diverse array of pathogens is primarily conveyed by the amino acid (aa) sequence of the hypervariable complementarity-determining region 3 (CDR3) segments of the T cell receptor (TCR). In this study, we used a combination of multiplex-PCR, Illumina sequencing and IMGT/HighV-QUEST for a standardized analysis of the characteristics and polymorphisms of the T-cell receptor BV complementarity-determining region 3 (TCR BV CDR3) gene in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy donors (NC). We found the distributions of CDR3, VD indel, and DJ indel lengths to be comparable between the SLE and NC groups. The degree of clonal expansion in the SLE group was significantly greater than in the NC group, and the expression levels of 10 TRβV segments and 6 TRβJ segments were also significantly different in the SLE group. Regarding public T cell responses, 3CDR3 DNA sequences and 4 aa sequences were shared by all SLE patients and may serve as biomarkers for SLE disease risk, diagnosis and/or prognosis.
authors.affiliation: Nephrology Department of Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, 541002 Guilin, Guangxi, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Sui
authors.affiliation: College of Life Science, Guangxi Normal University, 541004 Guilin, Guangxi, China.
authors.forename: Xianliang
authors.initials: X
authors.lastname: Hou
authors.affiliation: Nephrology Department of Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, 541002 Guilin, Guangxi, China.
authors.forename: Guimian
authors.initials: G
authors.lastname: Zou
authors.affiliation: Nephrology Department of Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, 541002 Guilin, Guangxi, China.
authors.forename: Wenti
authors.initials: W
authors.lastname: Che
authors.affiliation: Nephrology Department of Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, 541002 Guilin, Guangxi, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Life Science, Guangxi Normal University, 541004 Guilin, Guangxi, China.
authors.forename: Can
authors.initials: C
authors.lastname: Zheng
authors.affiliation: College of Life Science, Guangxi Normal University, 541004 Guilin, Guangxi, China.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Life Science, Guangxi Normal University, 541004 Guilin, Guangxi, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: College of Life Science, Guangxi Normal University, 541004 Guilin, Guangxi, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Wei
authors.affiliation: Nephrology Department of Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, 541002 Guilin, Guangxi, China.
authors.forename: Liusheng
authors.initials: L
authors.lastname: Lai
authors.affiliation: Nephrology Department of Guilin 181st Hospital, Guangxi Key laboratory of Metabolic Diseases Research, 541002 Guilin, Guangxi, China. Electronic address: xm183647168@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D016022:Case-Control Studies; D049109:Cell Proliferation; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D013601:T-Lymphocytes / Q000473:pathology; D055815:Young Adult
chemical_list: D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta
keywords: Next generation sequencing; Systemic lupus erythematosus; T cell receptor