title: Developmental validation of the GlobalFiler(®) Express PCR Amplification Kit: A 6-dye multiplex assay for the direct amplification of reference samples.
abstract: In order to increase the power of discrimination, reduce the possibility of adventitious matches, and expand global data sharing, the CODIS Core Loci Working Group made a recommendation to expand the CODIS core loci from the "required" 13 loci to 20 plus three additional "highly recommended" loci. The GlobalFiler(®) Express Kit was designed to incorporate all 20 required and 3 highly recommended loci along with a novel male-specific Y insertion/deletion marker. The GlobalFiler(®) Express Kit allows simultaneous amplification of the following loci: D3S1358, vWA, D16S539, CSF1PO, TPOX, Yindel, AMEL, D8S1179, D21S11, D18S51, DYS391, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, and D2S1338. The kit enables direct amplification from blood and buccal samples stored on paper or swab and the chemistry features an optimized PCR protocol that yields time to results in less than an hour. Developmental validation testing followed SWGDAM guidelines and demonstrated the quality and robustness of the GlobalFiler(®) Express Kit over a number of variables. The validation results demonstrate that the 24-locus multiplex kit is a robust and reliable identification assay as required for forensic DNA typing and databasing.
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA. Electronic address: dennis.wang1@lifetech.com.
authors.forename: Dennis Y
authors.initials: DY
authors.lastname: Wang
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.
authors.forename: Siddhita
authors.initials: S
authors.lastname: Gopinath
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Lagacé
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.
authors.forename: Wilma
authors.initials: W
authors.lastname: Norona
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.
authors.forename: Lori K
authors.initials: LK
authors.lastname: Hennessy
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.
authors.forename: Marc L
authors.initials: ML
authors.lastname: Short
authors.affiliation: Thermo Fisher Scientific Inc., 180 Oyster Point Blvd., South San Francisco, CA 94080, USA.
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Mulero
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D009711:Nucleotides / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D004396:Coloring Agents; D009711:Nucleotides
keywords: Direct amplification; Forensic validation studies; GlobalFiler(®) Express Kit; Rapid identification; STR