title: Development and validation of a modified QuEChERS protocol coupled to LC-MS/MS for simultaneous determination of multi-class antibiotic residues in honey.
abstract: LC-MS/MS assay was developed and validated according to EU guidelines for determination of nitrofuran metabolites and nitroimidazole residues in honey. Crude samples were acid-treated to liberate matrix-bound residues and a modified QuEChERS protocol was employed. Nitrofurantoin, furazolidone, furaltadone and nitrofurazone were determined via analysis of their metabolites AHD, AOZ, AMOZ and SEM, respectively while nitroimidazole residues; ronidazole (RNZ) and dimetridazole (DMZ) were determined directly. For all analytes, neat standard calibration curves, after correction for matrix effect were successfully employed. Decision limit (CCα) and detection capability (CCβ) were below the MRPL for nitrofurans (1.00 μg kg(-1)) and the recommended concentration for nitroimidazole (3.00 μg kg(-1)), respectively. The CCα, CCβ, percentage recovery and CV% ranges were 0.12-0.74 μg kg(-1), 0.21-1.27 μg kg(-1), 90.96-104.80% and 2.65-12.58%, respectively. This work is part of the national initiative for establishing a national monitoring program for drug residues in Egyptian honey.
authors.affiliation: Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food, Agricultural Research Center, Ministry of Agriculture and Land Reclamation, Giza, Egypt.
authors.forename: Amr H
authors.initials: AH
authors.lastname: Shendy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Bioanalysis Research Group, Faculty of Pharmacy, Cairo University, Cairo, Egypt. Electronic address: medhat.alghobashy@cu.edu.eg.
authors.forename: Medhat A
authors.initials: MA
authors.lastname: Al-Ghobashy
authors.affiliation: Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food, Agricultural Research Center, Ministry of Agriculture and Land Reclamation, Giza, Egypt.
authors.forename: Sohair A
authors.initials: SA
authors.lastname: Gad Alla
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University, Cairo, Egypt.
authors.forename: Hayam M
authors.initials: HM
authors.lastname: Lotfy
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D006722:Honey / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents
keywords: AHD; AMOZ; AOZ; Dimetridazole; Honey; LC–MS/MS; Nitrofuran; Nitroimidazoles; Ronidazole; SEM; Veterinary drug residues