title: Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin.
abstract: Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional characteristics of browning. Using this method we could validate mouse data in human samples demonstrating the effectiveness of irisin to induce "beige" differentiation of subcutaneous white adipocytes.
authors.affiliation: MTA-DE Stem Cells, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Endre
authors.initials: E
authors.lastname: Kristóf
authors.affiliation: Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Quang-Minh
authors.initials: QM
authors.lastname: Doan-Xuan
authors.affiliation: MTA-DE Lendület Laboratory of Cellular Metabolism Research Group, Research Center for Molecular Medicine, Department of Medical Chemistry, University of Debrecen, Debrecen, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Bai
authors.affiliation: Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bacso
authors.affiliation: MTA-DE Stem Cells, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Fésüs
mesh_terms: D052437:Adipocytes, Brown / Q000166:cytology* / Q000378:metabolism*; D052438:Adipocytes, White / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D047569:Laser Scanning Cytometry / Q000379:methods*; D051379:Mice
chemical_list: C577593:FNDC5 protein, human; D005353:Fibronectins