title: Inhibitory effects of a dendritic cell vaccine loaded with radiation-induced apoptotic tumor cells on tumor cell antigens in mouse bladder cancer.
abstract: Herein, the preparation of a dendritic cell (DC) vaccine with radiation-induced apoptotic tumor cells and its immunological effects on bladder cancer in C57BL/6 mice was investigated. We used radiation to obtain a MB49 cell antigen that was sensitive to bone marrow-derived DCs to prepare a DC vaccine. An animal model of tumor-bearing mice was established with the MB49 mouse bladder cancer cell line. Animals were randomly allocated to an experimental group or control group. DC vaccine or phosphate-buffered saline was given 7 days before inoculation with tumor cells. Each group consisted of 2 subgroups in which tumor volume and the survival of tumor-bearing mice were recorded. Tumor volumes and average tumor masses of mice administered DC vaccine loaded with radiation-induced apoptotic cells were significantly lower than those in the control group (P < 0.01). Survival in the experimental group was also longer than that in the control group, and 2 mice survived without tumor formation. In the DC vaccine group, 2 mice were alive without tumor growth after 30 days, and no tumor was observed at 30 days after subcutaneous inoculation of MB49 cells. The DC vaccine loaded with radiation-induced apoptotic tumor cells had an anti-tumor effect and was associated with increased survival in a bladder cancer model in mice.
authors.affiliation: Department of Urology, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Xie
authors.affiliation: Department of Urology Research Institute, Huashan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of Urology Research Institute, Huashan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Hou
authors.affiliation: Department of Urology, Jinshan Hospital Affiliated to Fudan University, Shanghai, China chgan305@163.com.
authors.forename: G
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D017209:Apoptosis / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000166:cytology; D019496:Cancer Vaccines / Q000276:immunology*; D002452:Cell Count; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008810:Mice, Inbred C57BL; D047368:Tumor Burden; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology*; D014965:X-Rays
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines