title: A Versatile Approach Towards Nucleobase-Modified Aptamers.
abstract: A novel and versatile method has been developed for modular expansion of the chemical space of nucleic acid libraries, thus enabling the generation of nucleobase-modified aptamers with unprecedented recognition properties. Reintroduction of the modification after enzymatic replication gives broad access to many chemical modifications. This wide applicability, which is not limited to a single modification, will rapidly advance the application of in vitro selection approaches beyond what is currently feasible and enable the generation of aptamers to many targets that have so far not been addressable.
authors.affiliation: Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn (Germany).
authors.forename: Fabian
authors.initials: F
authors.lastname: Tolle
authors.affiliation: Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn (Germany).
authors.forename: Gerhard M
authors.initials: GM
authors.lastname: Brändle
authors.affiliation: Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn (Germany).
authors.forename: Daniel
authors.initials: D
authors.lastname: Matzner
authors.affiliation: Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn (Germany). gmayer@uni-bonn.de.
authors.forename: Günter
authors.initials: G
authors.lastname: Mayer
mesh_terms: D052157:Aptamers, Nucleotide; D009696:Nucleic Acids
chemical_list: D052157:Aptamers, Nucleotide; D009696:Nucleic Acids
keywords: GFP; SELEX; aptamers; molecular evolution; nucleic acids