title: Antiarthritic activity of a polyherbal formulation against Freund's complete adjuvant induced arthritis in Female Wistar rats.
abstract: OBJECTIVE
  To formulate a polyherbal formulation and evaluate its antiarthritic activity against Freund's complete adjuvant induced arthritis in Female Wistar rats.
  
  
  METHODS
  Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including arthritis. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla , whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. Arthritis was induced in female Wistar rats using Freund's complete adjuvant (FCA), and the antiarthritic effect of polyherbal formulation was studied at doses of 250 and 500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, blood samples were collected for biochemical and hematological analysis. The radiological examination was carried out before terminating the study.
  
  
  RESULTS
  Polyherbal formulation showed significant antiarthritic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of indomethacin. The antiarthritic activity of polyherbal formulation is supported by biochemical and hematological analysis.
  
  
  CONCLUSIONS
  The polyherbal formulation showed signinicant antiarthritic activity against FCA-induced arthritis in female Wistar rats.
authors.affiliation: Department of Pharmacology, Vasavi Institute of Pharmaceutical Sciences, Peddapalli, Kadapa, Andhra Pradesh, India.
authors.forename: R Ramesh
authors.initials: RR
authors.lastname: Petchi
authors.affiliation: Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia.
authors.forename: S
authors.initials: S
authors.lastname: Parasuraman
authors.affiliation: Department of Pharmacology, Ultra College of Pharmacy, Madurai, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Vijaya
authors.affiliation: Department of Pharmacology, Vasavi Institute of Pharmaceutical Sciences, Peddapalli, Kadapa, Andhra Pradesh, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Gopala Krishna
authors.affiliation: Department of Pharmacology, Vasavi Institute of Pharmaceutical Sciences, Peddapalli, Kadapa, Andhra Pradesh, India.
authors.forename: M Kiran
authors.initials: MK
authors.lastname: Kumar
keywords: Arthritis; Fingerprint analysis; Glycosmis pentaphylla; Mangifera indica; Tridax procumbens