title: 454-Pyrosequencing Reveals Microbial Community Structure and Composition in a Mesophilic UAFB System Treating PTA Wastewater.
abstract: To well understand the community structure and composition of mesophilic microorganisms in anaerobic system fed with PTA wastewater, an up-flow anaerobic fixed bed reactor was continuously run at 33 and 37 °C for 75 and 60 days, respectively. Both fluorescence in situ hybridization analysis and 454-pyrosequencing were applied to investigate the microbial distinction within mesophilic ranges. A preferable performance was achieved at 37 than 33 °C. The taxonomic complexities of two samples were further compared at phylum, class, and genus levels. Notably, microbial diversity differed a lot and the change of populations was observed mainly in the shared OTUs. Genus level analysis showed that when temperature was increased to 37 °C, the abundance of Thauera and Hydrogenophaga (β-Proteobacteria) decreased by 93.75 and 61.47 %, respectively, whereas that of Syntrophorhabdus (δ-Proteobacteria) increased from 4.93 to 16.01 %. Furthermore, the dominant archaeal Methanobacterium at both temperatures indicated the prevailing contribution of hydrogenotrophic methanogens in mesophilic anaerobic system.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China. makaili@126.com.
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China. xkli312@163.com.
authors.forename: Xiang-Kun
authors.initials: XK
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China.
authors.forename: He-Xi
authors.initials: HX
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China.
authors.forename: Ling-Wei
authors.initials: LW
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics; D019149:Bioreactors / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D017404:In Situ Hybridization, Fluorescence; D054892:Metagenome; D064307:Microbiota; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology; D013696:Temperature; D062065:Wastewater / Q000382:microbiology*
chemical_list: D012722:Sewage; D062065:Wastewater
keywords: 454-pyrosequencing; FISH; Mesophilic anaerobic wastewater treatment; Microbial community; UAFB