title: Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.
abstract: The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Mihailović
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Arambašić Јovanović
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Uskoković
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Nevena
authors.initials: N
authors.lastname: Grdović
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Dinić
authors.affiliation: Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Senka
authors.initials: S
authors.lastname: Vidović
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Goran
authors.initials: G
authors.lastname: Poznanović
authors.affiliation: Biotechnical Faculty, University of Bihać, Kulina Bana 2, 77000 Bihać, Bosnia and Herzegovina.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Mujić
authors.affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Melita
authors.initials: M
authors.lastname: Vidaković
mesh_terms: D000363:Agaricales; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase; D054377:Chemokine CXCL12 / Q000187:drug effects / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006442:Glycated Hemoglobin / Q000187:drug effects / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D002374:Catalase; D054377:Chemokine CXCL12; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D007328:Insulin; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase; D014280:Triglycerides; C575935:CXCL12 protein, rat