title: A Comprehensive Inter-Tissue Crosstalk Analysis Underlying Progression and Control of Obesity and Diabetes.
abstract: Obesity is a metabolic state associated with excess of positive energy balance. While adipose tissues are considered the major contributor for complications associated with obesity, they influence a variety of tissues and inflict significant metabolic and inflammatory alterations. Unfortunately, the communication network between different cell-types responsible for such systemic alterations has been largely unexplored. Here we study the inter-tissue crosstalk during progression and cure of obesity using multi-tissue gene expression data generated through microarray analysis. We used gene expression data sets from 10 different tissues from mice fed on high-fat-high-sugar diet (HFHSD) at various stages of disease development and applied a novel analysis algorithm to deduce the tissue crosstalk. We unravel a comprehensive network of inter-tissue crosstalk that emerges during progression of obesity leading to inflammation and insulin resistance. Many of the crosstalk involved interactions between well-known modulators of obesity and associated pathology like inflammation. We then used similar datasets from mice that in addition to HFHSD were also administered with a herbal concoction known to circumvent the effects of HFHSD in the diet induced model of obesity in mice. We propose, the analysis presented here could be applied to understand systemic details of several chronic diseases.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi-110016.
authors.forename: Pawan
authors.initials: P
authors.lastname: Samdani
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi-110016.
authors.forename: Meet
authors.initials: M
authors.lastname: Singhal
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sinha
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067.
authors.forename: Parul
authors.initials: P
authors.lastname: Tripathi
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067.
authors.forename: Sachin
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067.
authors.forename: Kamiya
authors.initials: K
authors.lastname: Tikoo
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067.
authors.forename: Kanury V S
authors.initials: KV
authors.lastname: Rao
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Kumar
mesh_terms: D000818:Animals; D003198:Computer Simulation; D003920:Diabetes Mellitus / Q000209:etiology / Q000378:metabolism*; D059305:Diet, High-Fat / Q000379:methods; D004041:Dietary Fats / Q000378:metabolism*; D019422:Dietary Sucrose / Q000378:metabolism*; D018450:Disease Progression; D051379:Mice; D008954:Models, Biological; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D009928:Organ Specificity; D020543:Proteome / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D004041:Dietary Fats; D019422:Dietary Sucrose; D020543:Proteome