title: Potential Use of a Weak Ethylene Receptor Mutant, Sletr1-2, as Breeding Material To Extend Fruit Shelf Life of Tomato.
abstract: Mutations in the ethylene receptor gene (SlETR1), Sletr1-1 and Sletr1-2, are effective in reducing ethylene sensitivity and improving fruit shelf life. In this study the effect of Sletr1-1 and Sletr1-2 mutations was investigated in F1 hybrid lines. These two mutants and control were crossed with four commercial pure-line tomatoes. The Sletr1-1 mutation showed undesirable pleiotropic effects in the F1 hybrid lines. The Sletr1-2 mutation was effective in improving fruit shelf life of F1 hybrid lines for 4-5 days longer. It was also effective in improving fruit firmness without change in fruit size, ethylene production, respiration rate, and total soluble solids or a great reduction in fruit color, lycopene, and β-carotene, although the titratable acidity was increased by Sletr1-2 mutation. These results indicate that the Sletr1-2 mutant allele has the potential to improve fruit shelf life via incorporation in tomato breeding programs.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba, 305-8572 Japan.|Department of Agronomy, Faculty of Agriculture, Padjadjaran University , Bandung, 45363 Indonesia.
authors.forename: Syariful
authors.initials: S
authors.lastname: Mubarok
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba, 305-8572 Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Okabe
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba, 305-8572 Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Fukuda
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba, 305-8572 Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Ariizumi
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba, 305-8572 Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
mesh_terms: D001947:Breeding; D061353:Food Storage; D005638:Fruit / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant
keywords: Sletr1-1; Sletr1-2; ethylene; fruit shelf life; tomato