title: Feasibility of using a translucid inorganic hydrogel to build a biosensor using immobilized algal cells.
abstract: Anthropic activities generate contaminants, as pesticides and other pollutants, in the aquatic environment which present a real threat to ecosystems and human health. Thus, monitoring tools become essential for water managers to detect these chemicals before the occurrence of adverse effects. In this aim, algal cell biosensors, based on photosystem II activity measurement, have been designed for several years in previous studies. In this work, we study a new immobilization technique of algal cells in the aim of improving the performance of these biosensors. Immobilization was here achieved by encapsulation in a hybrid alginate/silica translucid hydrogel. The feasibility of this process was here assessed, and the biosensor designed was tested on the detection of chemicals in urban rainwaters.
authors.affiliation: ENTPE, Université de Lyon, UMR 5023, 3 rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France. claude.durrieu@entpe.fr.
authors.forename: Claude
authors.initials: C
authors.lastname: Durrieu
authors.affiliation: ENTPE, Université de Lyon, UMR 5023, 3 rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France.
authors.forename: Yannis
authors.initials: Y
authors.lastname: Ferro
authors.affiliation: INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pab. II, C1428EHA, Buenos Aires, Argentina.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Perullini
authors.affiliation: ENTPE, Université de Lyon, UMR 5023, 3 rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Gosset
authors.affiliation: INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pab. II, C1428EHA, Buenos Aires, Argentina.
authors.forename: Matías
authors.initials: M
authors.lastname: Jobbágy
authors.affiliation: INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pab. II, C1428EHA, Buenos Aires, Argentina.
authors.forename: Sara A
authors.initials: SA
authors.lastname: Bilmes
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D018914:Cells, Immobilized; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010575:Pesticides / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis; D014881:Water Supply
chemical_list: D010575:Pesticides; D014874:Water Pollutants, Chemical; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: Algae; Biosensors; Encapsulation; Pesticides; Photosystem II