title: [Controlled Release of Low Molecular Protein Insulin-like Growth Factor-1 through Self-Assembling Peptide Hydrogel with Biotin Sandwich Approach].
abstract: Since the release rate of protein in hydrogels is directly dependent upon the size of the protein and the hydrogel, how to deliver low molecular weight protein for prolonged periods has always been a problem. In this article, we present a usage of self-assembling peptide (P3) with the RGD epitope on its N terminus. The concentration of the released insulin-like growth factor 1 (IGF-1) was determined by UV-vis spectroscopy and the release kinetics suggested a notable reduction of the IGF-1 release rate. Cell entrapment experiments revealed that IGF-1 delivery by biotinylated nanofibers could promote the proliferation of the mouse chondrogenic ATDC5 cells when compared with cells embedded within nanofibers with untethered IGF-1.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Fan
authors.forename: Yuying
authors.initials: Y
authors.lastname: Wang
authors.forename: Limei
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D001710:Biotin; D002460:Cell Line; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D051379:Mice; D057139:Nanofibers; D009842:Oligopeptides / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020100:Hydrogels; D009842:Oligopeptides; D007334:Insulin-Like Growth Factor I; D001710:Biotin; C047981:arginyl-glycyl-aspartic acid