title: Accumulation of amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles in bone marrow: implications for local stromal cells.
abstract: OBJECTIVE
  First, it will be investigated if amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles (A-PVA-SPIONs) are suitable for MRI contrast enhancement in bone marrow. Second, the impact of A-PVA-SPION exposure in vivo on the viability and key functions of local bone marrow stromal cells (BMSCs) will be investigated.
  
  
  METHODS
  Animals were systemically injected with A-PVA-SPIONs, followed by a 7-day survival time. Accumulation of A-PVA-SPIONs was confirmed by MRI, histology and inductively coupled plasma optical emission spectrometry. BMSCs were isolated from bone marrow for in vitro assessment of their viability and regenerative key functions.
  
  
  RESULTS
  In this study, A-PVA-SPIONs were found to accumulate in bone marrow and increase the BMSCs' metabolic activity and migration rate.
  
  
  CONCLUSIONS
  A-PVA-SPIONs appear suitable for contrast enhancement in bone marrow while our data suggest an influence on the BMSCs biology that necessitates future research.
authors.affiliation: Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.|Berlin-Brandenburg School for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schulze
authors.affiliation: Department of Radiology, Geneva University Hospitals & University of Geneva, 1205 Geneva, Switzerland.|Department of Oral Biology, Faculty of Oral & Dental Medicine, Cairo University, Cairo, Egypt.
authors.forename: Azza
authors.initials: A
authors.lastname: Gramoun
authors.affiliation: Department of Radiology, Geneva University Hospitals & University of Geneva, 1205 Geneva, Switzerland.
authors.forename: Lindsey A
authors.initials: LA
authors.lastname: Crowe
authors.affiliation: Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Dienelt
authors.affiliation: Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
authors.forename: Tugba
authors.initials: T
authors.lastname: Akcan
authors.affiliation: Laboratory of Powder Technology, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofmann
authors.affiliation: Department of Radiology, Geneva University Hospitals & University of Geneva, 1205 Geneva, Switzerland.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Vallée
authors.affiliation: Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
authors.forename: Georg N
authors.initials: GN
authors.lastname: Duda
authors.affiliation: Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ode
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide; D011142:Polyvinyl Alcohol
keywords: MRI; bone marrow; bone marrow stromal cells; contrast enhancement; polyvinyl alcohol; superparamagnetic iron oxide nanoparticles