title: Genistein-induced mir-23b expression inhibits the growth of breast cancer cells.
abstract: OBJECTIVE
  Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.
  
  
  METHODS
  XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.
  
  
  RESULTS
  The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.
  
  
  CONCLUSIONS
  Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.
authors.affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.
authors.forename: Cigir Biray
authors.initials: CB
authors.lastname: Avci
authors.affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.
authors.forename: Sunde Yilmaz
authors.initials: SY
authors.lastname: Susluer
authors.affiliation: Department of Stem Cell, Health Science Institute, Ege University, Bornova, Izmir, Turkey.
authors.forename: Hasan Onur
authors.initials: HO
authors.lastname: Caglar
authors.affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.
authors.forename: Tugce
authors.initials: T
authors.lastname: Balci
authors.affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Aygunes
authors.affiliation: Department of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Dodurga
authors.affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gunduz
keywords: MCF-7; breast cancer; genistein; miRNA