title: Mineral content of the maternal diet influences ectopic mineralization in offspring of Abcc6(-/-) mice.
abstract: Ectopic mineralization disorders inflicting the connective tissues display a spectrum of severity, some developing in utero and being diagnosed by prenatal ultrasound. This study was designed to test the hypothesis that the mineral content of maternal diet can influence the mineralization in the offspring. Pregnant Abcc6(-/-) mice, on 2 different strain backgrounds, were maintained either on normal rodent diet or on "acceleration diet," rich in phosphate and low in magnesium, which has been previously shown to enhance the mineralization processes. The offspring were examined for mineralization by histopathology of various tissues and quantitated by chemical assay of calcium. The ectopic mineralization in the dermal sheath of vibrissae, a progressive biomarker of the overall mineralization, was readily detectable at the age of 4 weeks in the pups whose mothers were on the acceleration diet, while no evidence of mineralization was noted in those on normal diet. The mineralization of the vibrissae progressively increased when examined at 12 weeks of age. There was a significant reduction in urinary calcium and significant increase in urinary phosphorus concentrations both at 4 and 12 weeks of age in mice on the acceleration diet as compared to those on control diet. The results demonstrate that the mineral content of the maternal diet can influence ectopic mineralization in the offspring of mice genetically predisposed to ectopic mineralization (Abcc6(-/-)). These observations have implications for dietary management of pregnancies in which the fetus is diagnosed by prenatal ultrasound to have an ectopic mineralization disorder.
authors.affiliation: a Department of Dermatology and Cutaneous Biology ; The Sidney Kimmel Medical College; Thomas Jefferson University ; Philadelphia , PA USA.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Li
authors.affiliation: a Department of Dermatology and Cutaneous Biology ; The Sidney Kimmel Medical College; Thomas Jefferson University ; Philadelphia , PA USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Kingman
authors.affiliation: a Department of Dermatology and Cutaneous Biology ; The Sidney Kimmel Medical College; Thomas Jefferson University ; Philadelphia , PA USA.
authors.forename: Jouni
authors.initials: J
authors.lastname: Uitto
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D003238:Connective Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D018345:Mice, Knockout; D008903:Minerals / Q000008:administration & dosage / Q000494:pharmacology*; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D011247:Pregnancy; D011561:Pseudoxanthoma Elasticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D008903:Minerals; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C504743:Abcc6 protein, mouse
keywords: animal model; ectopic mineralization; heritable disorders; maternal diet; pseudoxanthoma elasticum