title: Synthesis of highly interconnected 3D scaffold from Arothron stellatus skin collagen for tissue engineering application.
abstract: The substrate which is avidly used for tissue engineering applications should have good mechanical and biocompatible properties, and all these parameters are often considered as essential for dermal reformation. Highly interconnected three dimensional (3D) wound dressing material with enhanced structural integrity was synthesized from Arothron stellatus fish skin (AsFS) collagen for tissue engineering applications. The synthesized 3D collagen sponge (COL-SPG) was further characterized by different physicochemical methods. The scanning electron microscopy analysis of the material demonstrated that well interconnected pores with homogeneous microstructure on the surface aids higher swelling index and that the material also possessed good mechanical properties with a Young's modulus of 0.89±0.2 MPa. Biocompatibility of the 3D COL-SPG showed 92% growth for both NIH 3T3 fibroblasts and keratinocytes. Overall, the study revealed that synthesized 3D COL-SPG from fish skin will act as a promising wound dressing in skin tissue engineering.
authors.affiliation: Bioproducts Lab, CSIR - Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Giriprasath
authors.initials: G
authors.lastname: Ramanathan
authors.affiliation: Bioproducts Lab, CSIR - Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Singaravelu
authors.affiliation: Bioproducts Lab, CSIR - Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: M D
authors.initials: MD
authors.lastname: Raja
authors.affiliation: Bioproducts Lab, CSIR - Central Leather Research Institute, Adyar, Chennai, India. Electronic address: suma67@gmail.com.
authors.forename: Uma Tiruchirapalli
authors.initials: UT
authors.lastname: Sivagnanam
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001684:Biological Dressings; D003094:Collagen / Q000648:ultrastructure*; D055119:Elastic Modulus; D015603:Keratinocytes / Q000502:physiology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D012867:Skin / Q000737:chemistry*; D013718:Tensile Strength; D023721:Tetraodontiformes / Q000033:anatomy & histology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003094:Collagen
keywords: Arothron stellatus; Biocompatibility and wound dressing; Biomaterials