title: Datasets from an interaction proteomics screen for substrates of the SCF(βTrCP) ubiquitin ligase.
abstract: An affinity purification-mass spectrometry (AP-MS) method was employed to identify novel substrates of the SCF(βTrCP) ubiquitin ligase. A FLAG-HA tagged version of the F-box protein βTrCP2, the substrate recognition subunit of SCF(βTrCP), was used as bait. βTrCP2 wild type and the two mutants βTrCP2-R447A and βTrCP2-ΔF were expressed and purified from HEK293T cells to be able to discriminate between potential substrates of SCF(βTrCP) and unspecific binders. Affinity-purified samples were analyzed by mass spectrometry-based proteomics, applying ultra-high performance liquid chromatography (UHPLC) coupled to high-resolution tandem mass spectrometry. The raw mass spectrometry data have been deposited to the PRIDE partner repository with the identifiers PXD001088 and PXD001224. The present dataset is associated with a research resource published in T.Y. Low, M. Peng, R. Magliozzi, S. Mohammed, D. Guardavaccaro, A.J.R. Heck, A systems-wide screen identifies substrates of the SCF(βTrCP) ubiquitin ligase. Sci. Signal. 7 (2014) rs8-rs8, 10.1126/scisignal.2005882.
authors.affiliation: Hubrecht Institute-KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Roberto
authors.initials: R
authors.lastname: Magliozzi
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 H Utrecht, The Netherlands ; Netherlands Proteomics Center, Padualaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Mao
authors.initials: M
authors.lastname: Peng
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 H Utrecht, The Netherlands ; Netherlands Proteomics Center, Padualaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Shabaz
authors.initials: S
authors.lastname: Mohammed
authors.affiliation: Hubrecht Institute-KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Daniele
authors.initials: D
authors.lastname: Guardavaccaro
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 H Utrecht, The Netherlands ; Netherlands Proteomics Center, Padualaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 H Utrecht, The Netherlands ; Netherlands Proteomics Center, Padualaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Teck Yew
authors.initials: TY
authors.lastname: Low
keywords: Affinity purification-mass spectrometry (AP-MS); F-box protein; Proteomics; SCF ubiquitin ligase; βTrCP