title: Optical coherence tomography for cross-sectional imaging of neural activity.
abstract: We report a functional optical coherence tomography cross-sectional scanner to detect neural activity using unmyelinated nerves dissected from squid. The nerves, unstained or stained with a voltage-sensitive dye, were imaged in a nerve chamber. Transient phase changes from backscattered light were detected during action potential propagation. The results show that the scanner can provide high spatiotemporal resolution cross-sectional images of neural activity ([Formula: see text]; [Formula: see text]; [Formula: see text] in [Formula: see text]). The advantage of this method compared to monitoring a single depth profile [Formula: see text] is a dramatic increase in the number of available sites that can be measured in two spatial dimensions [Formula: see text] with lateral scanning; therefore, the study demonstrates that two-dimensional monitoring of small-scale functional activity would also be feasible.
authors.affiliation: University of Minnesota , Department of Electrical and Computer Engineering, Minneapolis, Minnesota 55455, United States ; University of Minnesota , Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Yi-Jou
authors.initials: YJ
authors.lastname: Yeh
authors.affiliation: University of Minnesota , Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Black
authors.affiliation: University of Miami , Department of Physiology and Biophysics, Miami, Florida 33101, United States.
authors.forename: David
authors.initials: D
authors.lastname: Landowne
authors.affiliation: University of Minnesota , Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Taner
authors.initials: T
authors.lastname: Akkin
keywords: action potentials; functional imaging; phase measurement; voltage sensitive dye imaging