title: Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes.
abstract: Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40 V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5 × 10(-10) mol cm(-2) and 3.36 s(-1), respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10 μM - 2 mM with LOD of 4.1 μM, (2) 2 mM - 5.2 mM with LOD of 0.95 mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors.forename: Rajkumar
authors.initials: R
authors.lastname: Devasenathipathy
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors.forename: Veerappan
authors.initials: V
authors.lastname: Mani
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan. Electronic address: smchen78@ms15.hinet.net.
authors.forename: Shen-Ming
authors.initials: SM
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan. Electronic address: ws75624@ntut.edu.tw.
authors.forename: Sheng-Tung
authors.initials: ST
authors.lastname: Huang
authors.affiliation: Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Biomedical Instrument Technology Division, Instrument Technology Research Center, National Applied Research Laboratories, 20, R&D Rd. VI, Hsinchu Science Park, Hsinchu, Taiwan.
authors.forename: Tsung-Tao
authors.initials: TT
authors.lastname: Huang
authors.affiliation: Department of Biochemistry, College of Medicine, Taipei Medical University, No. 250, Wu-xing Street, Taipei 110, Taiwan.
authors.forename: Chun-Mao
authors.initials: CM
authors.lastname: Lin
authors.affiliation: Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Kuo-Yuan
authors.initials: KY
authors.lastname: Hwa
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors.forename: Ting-Yo
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors.forename: Bo-Jun
authors.initials: BJ
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004579:Electron Transport; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006046:Gold; D006108:Graphite; D053768:Metal Nanoparticles / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000648:ultrastructure; D015203:Reproducibility of Results
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Carbon nanotubes; Electrochemistry; Glucose oxidase; Gold nanoparticles; Graphene