title: Concurrent measurement of cellular turbidity and hemoglobin to evaluate the antioxidant activity of plants.
abstract: In past decades, a multitude of analytical methods for measuring antioxidant activity of plant extracts has been developed. However, when using methods to determine hemoglobin released from human erythrocytes treated with ginger extracts, we found hemoglobin concentrations were significantly higher than in untreated control samples. This suggests in the presence of antioxidants that measuring hemoglobin alone is not sufficient to determine hemolysis. We show concurrent measurement of erythrocyte concentration and hemoglobin is essential in such assays, and describe a new protocol based on simultaneous measurement of cellular turbidity and hemoglobin.
authors.affiliation: Department of Biology, Faculty of Life and Nature Sciences, Mohamed El Bachir El Ibrahimi University, Bordj Bou Arreridj 34000, Algeria. Electronic address: bellik_youva@yahoo.fr.
authors.forename: Yuva
authors.initials: Y
authors.lastname: Bellik
authors.affiliation: Associated Laboratory in Marine Ecosystems and Aquacultural, Faculty of Life and Nature Sciences, Abderrahmane Mira University, Bejaia 06000, Algeria.
authors.forename: Mokrane
authors.initials: M
authors.lastname: Iguer-Ouada
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D015227:Lipid Peroxidation; D009391:Nephelometry and Turbidimetry; D010936:Plant Extracts / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood
chemical_list: D000975:Antioxidants; D006454:Hemoglobins; D010936:Plant Extracts; D002374:Catalase; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Antioxidant activity; Erythrocytes; Ginger extracts; Hemoglobin; Hemolysis test; Oxidative stress