title: Rapid quantification of Staphylococcus aureus from endotracheal aspirates of ventilated patients: a proof-of-concept study.
abstract: Major concern for intubated patients is ventilator-associated pneumonia (VAP). Early detection of VAP and its causative microorganism(s) is a key challenge for clinicians. Diagnosis is based on clinical, radiological, and microbiological elements, the latter being provided 24-48h after sampling. According to practices, clinicians can sample endotracheal aspirates (ETAs) so as to check for patient colonization or perform ETA in case of VAP suspicion. In this proof-of-concept study, we report the evaluation of a semiautomated molecular method to rapidly quantify Staphylococcus aureus, one of the most involved microorganisms in VAP, directly from raw ETA samples. After evaluation using artificial ETA samples, our method was applied on 40 clinical ETA samples. All S. aureus-positive samples were successfully detected and quantified. Our method can provide an efficient sample preparation protocol for all raw ETA samples, combined with an accurate quantification of the bacterial load, in less than 3h 30min.
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Lacroix
authors.affiliation: Inserm, UMR 1092, Limoges, Univ. Limoges, UMR 1092, CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Barraud
authors.affiliation: Intensive Care Unit/Inserm CIC1435, CHU Limoges, 2 avenue Martin Luther King, 87042 Limoges Cedex, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Clavel
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Filiputti
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Prudent
authors.affiliation: Intensive Care Unit/Inserm CIC1435, CHU Limoges, 2 avenue Martin Luther King, 87042 Limoges Cedex, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: François
authors.affiliation: Inserm, UMR 1092, Limoges, Univ. Limoges, UMR 1092, CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Marie Cécile
authors.initials: MC
authors.lastname: Ploy
authors.affiliation: bioMérieux SA, Chemin de l'Orme, 69280 Marcy l'Etoile, France.
authors.forename: Marie-Astrid
authors.initials: MA
authors.lastname: Jestin
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Rodrigue
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Pachot
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France.
authors.forename: Javier
authors.initials: J
authors.lastname: Yugueros-Marcos
authors.affiliation: Medical Diagnostic Discovery Department, bioMérieux SA, Centre Christophe Mérieux, 5 rue des berges, 38024 Grenoble cedex 1, France. Electronic address: virginie.moucadel@biomerieux.com.
authors.forename: Virginie
authors.initials: V
authors.lastname: Moucadel
mesh_terms: D058491:Bacterial Load / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D053717:Pneumonia, Ventilator-Associated / Q000175:diagnosis*; D011446:Prospective Studies; D012121:Respiration, Artificial / Q000009:adverse effects*; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013997:Time Factors
keywords: Endotracheal aspirate (ETA); Molecular diagnosis; Staphylococcus aureus; Ventilator-associated pneumonia (VAP)