title: Lung morphometry changes in prevention of airway remodeling by protocatechuic aldehyde in asthmatic mice.
abstract: Airway remodeling can lead to irreversible airflow obstruction and persistent airway hyper-responsiveness, which is the pathological basis of refractory asthma. To investigate the preventive effect of protocatechuic aldehyde on airway remodeling in asthmatic mice by lung morphometry methods. BALB/c mice were used to establish model of airway remodeling by ovalbumin (OVA) inhalation. Bronchoalveolar lavage fluid (BALF) were collected for eosinophils (EOS) count and detection of interleukin 4 (IL-4), interleukin-13 (IL-13) and interferon (IFN-γ) content. The left lung pathological sections were performed HE, AB-PAS and Masson staining. The epithelial lamina thickness of the left main bronchus (Re), the smooth muscle layer thickness (Rm), the number of goblet cells and goblet cell area percentage (%Ac) and gas side of the road and vascular collagen deposition (%Aco, %Avc) situation were measured. Protocatechuic aldehyde gavage made the reduction of BALF EOS count. IL-4 and IL-13 levels also decreased, while the IFN-γ level increased. The left main bronchus Re, Rm, goblet cell count, Ac% and Aco% and Avc% reduced. Protocatechuic aldehyde can significantly control airway inflammation and prevent airway remodeling.
authors.affiliation: Department of Human Anatomy, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Jiankai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Foreign Languages, Hubei University of Chinese Medicine Wuhan 430065, Hubei, China.
authors.forename: Mulan
authors.initials: M
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Qin
authors.affiliation: Department of Stomatology, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Human Anatomy, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Guangdong Medical University Zhanjiang 524023, Guangdong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Fu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University Guangdong, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Liao
keywords: Protocatechuic aldehyde; airway remodeling; asthma; lung morphometry