title: [Investigation of Protein Translocation Sec-System with Heterologous Gene Expression in Shewanella oneidensis MR-1 Bacterium Cells].
abstract: A comparison of the primary structures of the protein translocation Sec-system proteins in the Shewanella oneidensis MR-1 and Escherichia coli bacteria was carried out. The process of translocation of recombinant pro-enteroxins (SEB and SEH) from Staphylococcus aureus and pro-streptavidin (SAV) from Streptomyces avidinii in the S. oneidensis MR-1 and E. coli cell periplasm was studied. It was demonstrated that these marker proteins are transferred into the periplasmic space of the S. oneidensis MR-1 transformant strain cells. The identity of N-terminal amino acid sequences of mature recombinant SEB, SEH, and SAV proteins (generated during post-translation proteolysis of leader peptide by the Sec-system both in E. coli and S. oneidensis MR-1) was established.
authors.forename: N N
authors.initials: NN
authors.lastname: Mordkovich
authors.forename: N A
authors.initials: NA
authors.lastname: Okorokova
authors.forename: V P
authors.initials: VP
authors.lastname: Veiko
mesh_terms: D058947:Bacterial Secretion Systems; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D021381:Protein Transport / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D020592:Shewanella / Q000235:genetics / Q000378:metabolism
chemical_list: D058947:Bacterial Secretion Systems; D004768:Enterotoxins; D011994:Recombinant Proteins; C402489:Staphylococcal enterotoxin H; C031818:enterotoxin B, staphylococcal