title: Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation.
abstract: Production of monoacylglycerols (MAGs) rich in ω-3 polyunsaturated fatty acids (n-3 PUFAs) was conducted through short path distillation (SPD) of an acylglycerol mixture (containing 67% MAGs) produced by enzymatic glycerolysis of sardine oil with glycerol. A stepwise SPD process in a UIC KDL 5 system (vacuum 10(-3)mbar, feeding flow 1.0 mL/min) was proceeded: the first distillation performed at evaporator temperature (TE) of 110 °C to remove glycerol completely and most of FFAs; and the second distillation at optimized TE 155 °C; resulting in a stream distillate with 91% purity and 94% overall recovery of MAGs. This work also demonstrated that SPD is able to concentrate n-3 PUFAs in MAG form by distilling at proper TE e.g. 125 °C, where n-3 PUFAs are concentrated in the residues. Moreover, this work mapped out a complete processing diagram for scalable production of n-3 PUFAs enriched MAGs as potential food emulsifier and ingredient.
authors.affiliation: Department of Biotechnology and Food Science (Chemical Engineering Section), Pza. Misael Bañuelos, s/n, University of Burgos, 09001 Burgos, Spain; Department of Engineering, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus C, Denmark.
authors.forename: Ángela García
authors.initials: ÁG
authors.lastname: Solaesa
authors.affiliation: Department of Biotechnology and Food Science (Chemical Engineering Section), Pza. Misael Bañuelos, s/n, University of Burgos, 09001 Burgos, Spain. Electronic address: tersanz@ubu.es.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Sanz
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus C, Denmark.
authors.forename: Mia
authors.initials: M
authors.lastname: Falkeborg
authors.affiliation: Department of Biotechnology and Food Science (Chemical Engineering Section), Pza. Misael Bañuelos, s/n, University of Burgos, 09001 Burgos, Spain.
authors.forename: Sagrario
authors.initials: S
authors.lastname: Beltrán
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus C, Denmark. Electronic address: guo@eng.au.dk.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
mesh_terms: D057168:Distillation; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000737:chemistry*; D008049:Lipase / Q000378:metabolism; D050178:Monoglycerides / Q000032:analysis / Q000737:chemistry*; D013696:Temperature
chemical_list: D015525:Fatty Acids, Omega-3; D050178:Monoglycerides; C093666:Lipozyme; D008049:Lipase
keywords: Lipozyme 435; Monoacylglycerols (MAGs) glycerolysis; Omega-3 polyunsaturated fatty acids (n-3 PUFAs); Short path distillation (SPD)