title: Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.
abstract: The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.
authors.affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.
authors.forename: Jaya
authors.initials: J
authors.lastname: Kumari
authors.affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University , Beijing , China.
authors.forename: Zuobing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway.
authors.forename: Trilochan
authors.initials: T
authors.lastname: Swain
authors.affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Heng
authors.initials: H
authors.lastname: Chi
authors.affiliation: Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University , Beijing , China.
authors.forename: Cuijuan
authors.initials: C
authors.lastname: Niu
authors.affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway.
authors.forename: Jarl
authors.initials: J
authors.lastname: Bøgwald
authors.affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway.
authors.forename: Roy Ambli
authors.initials: RA
authors.lastname: Dalmo
keywords: A. salmonicida; Atlantic salmon; T-bet; gene expression; mucosal immunity