title: Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy.
abstract: Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties ofM. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy.
authors.affiliation: Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BR.
authors.forename: André Flores
authors.initials: AF
authors.lastname: Braga
authors.affiliation: Universidade de Santo Amaro, São Paulo, SP, BR.
authors.forename: Daniela Ferraz
authors.initials: DF
authors.lastname: Moretto
authors.affiliation: Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, BR.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gigliotti
authors.affiliation: Faculdade Anhanguera de Bauru, Bauru, SP, BR.
authors.forename: Mariela
authors.initials: M
authors.lastname: Peruchi
authors.affiliation: Instituto Lauro de Souza Lima, Bauru, SP, BR.
authors.forename: Fátima Regina
authors.initials: FR
authors.lastname: Vilani-Moreno
authors.affiliation: Departamento de Microbiologia e Imunologia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, BR.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
authors.affiliation: Instituto Lauro de Souza Lima, Bauru, SP, BR.
authors.forename: Ana Carla Pereira
authors.initials: AC
authors.lastname: Latini
authors.affiliation: Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, North Holland, NL.
authors.forename: Anand
authors.initials: A
authors.lastname: Iyer
authors.affiliation: Instituto Lauro de Souza Lima, Bauru, SP, BR.
authors.forename: Pranab Kumar
authors.initials: PK
authors.lastname: Das
authors.affiliation: Instituto Lauro de Souza Lima, Bauru, SP, BR.
authors.forename: Vânia Nieto Brito de
authors.initials: VN
authors.lastname: Souza
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D018664:Interleukin-12 / Q000276:immunology; D015440:Leprosy, Lepromatous / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D012189:Retrospective Studies
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D018664:Interleukin-12