title: Investigation of the surfactant type and concentration effect on the retention factors of glutathione and its analogues by micellar electrokinetic chromatography.
abstract: In the present study, a micellar electrokinetic chromatographic method was used to determine the retention factors of hydrophilic monomeric and homodimeric forms of glutathione analogues. Ionic-liquid-based surfactant, 1-tetradecyl-3-methylimidazolium chloride, as well as cetyltrimethylammonium bromide and phosphate buffer (pH 7.4) were employed in the experiments. Since the studied peptides possess a negative charge under physiological conditions, it is expected that the peptides interact with the oppositely charged 1-tetradecyl-3-methylimidazolium chloride and cetyltrimethylammonium bromide micelles via hydrophobically assisted electrostatic forces. The dependence of the retention factor on the micellar concentration of 1-tetradecyl-3-methylimidazolium chloride and cetyltrimethylammonium bromide is nonlinear and the obtained curves converge to a limiting value. The retention factor values of GSH analogues were in the range of 0.36-2.22 for glutathione analogues and -1.21 to 0.37 for glutathione when 1-tetradecyl-3-methylimidazolium chloride was used. When cetyltrimethylammonium bromide was employed, the retention factor values were in the range of 0.27-2.17 for glutathione analogues and -1.22 to 0.06 for glutathione. If sodium dodecyl sulfate was used, the retention factor values of glutathione analogues with carnosine moiety were in the range of -1.54 to 0.38.
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Jana
authors.initials: J
authors.lastname: Kazarjan
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Riina
authors.initials: R
authors.lastname: Mahlapuu
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Mats
authors.initials: M
authors.lastname: Hansen
authors.affiliation: Institute of Biomedicine and Translational Medicine, Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Ursel
authors.initials: U
authors.lastname: Soomets
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Mihkel
authors.initials: M
authors.lastname: Kaljurand
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Merike
authors.initials: M
authors.lastname: Vaher
mesh_terms: D000975:Antioxidants / Q000032:analysis; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000032:analysis*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007093:Imidazoles; D052578:Ionic Liquids; D008823:Micelles; D009842:Oligopeptides / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents
chemical_list: C521426:1-tetradecyl-3-methylimidazolium; D000975:Antioxidants; D002593:Cetrimonium Compounds; D007093:Imidazoles; D052578:Ionic Liquids; D008823:Micelles; D009842:Oligopeptides; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D005978:Glutathione; D000077286:Cetrimonium
keywords: Glutathione analogues; Ionic-liquid-based surfactants; Micellar electrokinetic chromatography; Retention factors; UPF peptides