title: Rapid and quantitative detection of 4(5)-methylimidazole in caramel colours: A novel fluorescent-based immunochromatographic assay.
abstract: A novel fluorescence-based immunochromatographic assay (ICA) for rapid detecting 4(5)-methylimidazole (4-MI) is presented in this study. In our work, the conjugates of fluorescent microspheres (FMs) and 4-MI monoclonal antibody were used as probe for ICA. Under optimal conditions, a standard curve of ICA-based detection of 4-MI was developed, linear detection ranged from 0.50 to 32.0 mg/L. The cross-reactivities were observed less than 3.93% by detecting 6 selected structural analogues of 4-MI. The recoveries of 4-MI in caramels detection were ranged from 82.85% to 102.31%, with the coefficient of variation (n = 3) below 9.06%. Quantitative comparison of the established fluorescence-based ICA with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) analysis of real caramel colour samples indicated a good correlation among the methods. Therefore, our developed fluorescence-based ICA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of 4-MI in food safety control.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xinlan
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Huang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; State Key Laboratory of Pulp and Paper Engineering, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China. Electronic address: shujuanyu8@gmail.com.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D007093:Imidazoles / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002241:Carbohydrates; D007093:Imidazoles; C569050:caramel coloring; C023439:4-methylimidazole
keywords: 1,2-Dimethylimidazole (PubChem CID: 5617); 1-Ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride (CID: 2723939); 1-Methylimidazole (PubChem CID: 1390); 2-(N-morpholino)ethanesulfonic acid (CID: 78165); 2-Methylimidazole (PubChem CID: 12749); 4(5)-Methylimidazole; 4(5)-Methylimidazole (PubChem CID: 13195); 4-Nitrobenzyl bromide (PubChem CID: 66011); 5-Benzimidazolecarboxylic acid (PubChem CID: 459456); Fluorescent microspheres; Histamine (PubChem CID: 774); Immunochromatographic assay; N-hydroxysuccinimide (CID: 80170)