title: Serotonin Depletion Does not Modify the Short-Term Brain Hypometabolism and Hippocampal Neurodegeneration Induced by the Lithium-Pilocarpine Model of Status Epilepticus in Rats.
abstract: It has been reported that fluoxetine, a selective serotonin (5-hydroxytryptamine; 5-HT) reuptake inhibitor, has neuroprotective properties in the lithium-pilocarpine model of status epilepticus (SE) in rats. The aim of the present study was to investigate the effect of 5-HT depletion by short-term administration of p-chlorophenylalanine (PCPA), a specific tryptophan hydroxylase inhibitor, on the brain hypometabolism and neurodegeneration induced in the acute phase of this SE model. Our results show that 5-HT depletion did modify neither the brain basal metabolic activity nor the lithium-pilocarpine-induced hypometabolism when evaluated 3 days after the insult. In addition, hippocampal neurodegeneration and astrogliosis triggered by lithium-pilocarpine were not exacerbated by PCPA treatment. These findings point out that in the early latent phase of epileptogenesis, non-5-HT-mediated actions may contribute, at least in some extent, to the neuroprotective effects of fluoxetine in this model of SE.
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain. lgarciag@ucm.es.|Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain. lgarciag@ucm.es.
authors.forename: Luis
authors.initials: L
authors.lastname: García-García
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain.
authors.forename: Ahmed Anis
authors.initials: AA
authors.lastname: Shiha
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Bascuñana
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: de Cristóbal
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain.
authors.forename: Rubén
authors.initials: R
authors.lastname: Fernández de la Rosa
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain.|Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Delgado
authors.affiliation: Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII nº 1, 28040, Madrid, Spain.|Instituto Tecnológico PET, C/Manuel Bartolomé Cossío nº 10, 28040, Madrid, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Pozo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D010134:Fenclonine; D005911:Gliosis / Q000473:pathology; D006624:Hippocampus / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D008094:Lithium; D008279:Magnetic Resonance Imaging; D008297:Male; D009410:Nerve Degeneration / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D010862:Pilocarpine; D049268:Positron-Emission Tomography; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000172:deficiency*; D013226:Status Epilepticus / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors
chemical_list: D010862:Pilocarpine; D012701:Serotonin; D008094:Lithium; D010134:Fenclonine
keywords: Fluoro-Jade C; Lithium–pilocarpine; Serotonin; Status epilepticus; [18F]FDG PET; p-Chlorophenylalanine