title: Wnt1-induced MAFK expression promotes osteosarcoma cell proliferation.
abstract: Osteosarcoma is one of the most common primary bone tumors in children and young adults. In this study, we investigated the role of musculoaponeurotic fibrosarcoma oncogene homolog K (MAFK) in osteosarcoma cell proliferation in vitro and the possible pathways that contributed to MAFK-related osteosarcoma development. We first reported that MAFK was expressed at low levels in an osteosarcoma cell line. Furthermore, a significant correlation between MAFK and the Wnt signaling pathway was observed in osteosarcoma by using a gene microarray assay. We found that expression of MAFK could be induced by Wnt1 in a dose-dependent manner. Furthermore, Wnt1-induced MAFK expression caused a significant increase of cell viability, whereas a Wnt pathway inhibitor, IWR-1-endo, abolished Wnt1-induced effects on MAFK. Finally, cell cycle analysis showed that enhanced cell proliferation might be attributed to re-distribution of the cell cycle. Together, our results suggested that Wnt1-induced MAFK expression promoted cell proliferation in MG63 cells, and that the role of MAFK in osteosarcoma might be closely linked to the Wnt signaling pathway.
authors.affiliation: Department of Orthopedics, The 117th Hospital of PLA, Hangzhou, Zhejiang, China.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The 117th Hospital of PLA, Hangzhou, Zhejiang, China.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, The 117th Hospital of PLA, Hangzhou, Zhejiang, China.
authors.forename: D-S
authors.initials: DS
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The 117th Hospital of PLA, Hangzhou, Zhejiang, China.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, The 117th Hospital of PLA, Hangzhou, Zhejiang, China zhaozfag@126.com.
authors.forename: Z-F
authors.initials: ZF
authors.lastname: Zhao
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D006801:Humans; D050985:MafK Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D060449:Wnt Signaling Pathway; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494695:MAFK protein, human; D050985:MafK Transcription Factor; D012333:RNA, Messenger; C495247:WNT1 protein, human; D051155:Wnt1 Protein