title: Umezawaea endophytica sp. nov., isolated from tobacco root samples.
abstract: Two Gram-staining positive, aerobic and non-motile actinomycete strains, designated YIM 2047X(T) and YIM 2047D, were isolated from tobacco root samples collected from Shiling county, Yunnan province, South-West China. The isolates grew at 15-40 °C, pH 6.0-8.0 and 0-4.5 % NaCl (w/v). The whole-cell hydrolysates contained meso-diaminopimelic acid, galactose, mannose, ribose and rhamnose. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major cellar fatty acids identified were iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0, C17:1 ω8c and C17:0. MK-9 (H4) was the predominant menaquinone. The genomic DNA G+C contents were 73.4 and 74.2 mol%, respectively. These chemotaxonomic data, together with their morphological properties, were consistent with the assignment of the two strains to the genus Umezawaea. They showed highest similarities to Umezawaea tangerina JCM 10302(T) on phylogenetic analysis of their 16S rRNA gene sequences and were found to form a coherent cluster. However, the DNA-DNA hybridization values of YIM 2047X(T) and YIM 2047D with U. tangerina JCM 10302(T) were 46.5 ± 3.1 and 57.5 ± 1.6 %, respectively; while the reassociation value between themselves was 80.4 ± 2.3 %. The results of physiological and biochemical tests allowed the two new isolates to be differentiated phenotypically from the recognized strain U. tangerina JCM 10302(T). On the basis of results from this polyphasic study, the strains were characterized as a novel species of the genus Umezawaea, for which the name Umezawaea endophytica sp. nov. is proposed. The type strain is YIM 2047X(T) (=KCTC 39538(T) = CPCC 204132(T)).
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chu
authors.forename: Bing-Bing
authors.initials: BB
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.forename: Salam
authors.initials: S
authors.lastname: Nimaichand
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014026:Nicotiana / Q000382:microbiology*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; D003960:Diaminopimelic Acid