title: Decelerating Mature Adipocyte Dedifferentiation by Media Composition.
abstract: The establishment of adipose tissue test systems is still a major challenge in the investigation of cellular and molecular interactions responsible for the pathogenesis of inflammatory diseases involving adipose tissue. Mature adipocytes are mainly involved in these pathologies, but rarely used in vitro, due to the lack of an appropriate culture medium which inhibits dedifferentiation and maintains adipocyte functionality. In our study, we showed that Dulbecco's Modified Eagle's Medium/Ham's F-12 with 10% fetal calf serum (FCS) reported for the culture of mature adipocytes favors dedifferentiation, which was accompanied by a high glycerol release, a decreasing release of leptin, and a low expression of the adipocyte marker perilipin A, but high expression of CD73 after 21 days. Optimized media containing FCS, biotin, pantothenate, insulin, and dexamethasone decelerated the dedifferentiation process. These cells showed a lower lipolysis rate, a high level of leptin release, as well as a high expression of perilipin A. CD73-positive dedifferentiated fat cells were only found in low quantity. In this work, we showed that mature adipocytes when cultured under optimized conditions could be highly valuable for adipose tissue engineering in vitro.
authors.affiliation: 1 Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart , Stuttgart, Germany .
authors.forename: Birgit
authors.initials: B
authors.lastname: Huber
authors.affiliation: 2 Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB , Stuttgart, Germany .|3 Process Analysis & Technology (PA&T), Reutlingen University , Reutlingen, Germany .
authors.forename: Petra J
authors.initials: PJ
authors.lastname: Kluger
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D054337:Cell Dedifferentiation / Q000187:drug effects*; D003470:Culture Media / Q000494:pharmacology*; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D002352:Carrier Proteins; D003470:Culture Media; D058851:GPI-Linked Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins; D015720:5'-Nucleotidase; C548241:NT5E protein, human