title: Prevalence and patterns of antimicrobial resistance among Escherichia coli isolated from Zambian dairy cattle across different production systems.
abstract: This study focused on the use of antibiotics on small, medium and commercial-sized dairy farms in the central region of Zambia and its relationship to antibiotic resistance in Escherichia coli. A stratified random sample of 104 farms was studied, representing approximately 20% of all dairy farms in the region. On each farm, faecal samples were collected from a random sample of animals and a standardised questionnaire on the usage of antibiotics was completed. An E. coli isolate was obtained from 98.67% (371/376) of the sampled animals and tested for resistance to six classes of antibiotics. The estimated prevalence of resistance across the different farming systems was: tetracycline (10.61; 95%CI: 7.40-13.82), ampicillin (6.02; 95%CI: 3.31-8.73), sulfamethoxazole/ trimethoprim (4.49; 95%CI: 2.42-6.56), cefpodoxime (1.91; 95%CI: 0.46-3.36), gentamicin (0.89; 95%CI: 0.06-1.84) and ciprofloxacin (0%). Univariate analyses indicated certain diseases, exotic breeds, location, farm size and certain management practices as risk factors for detection of resistance, whereas multivariate analyses showed an association with lumpy skin disease and a protective effect for older animals (>25 months). This study has provided novel insights into the drivers of antibiotic use and their association with antibiotic resistance in an under-studied region of Southern Africa.
authors.affiliation: 1] Divisions of Immunity and Infection, Veterinary Clinical Sciences [2] District Veterinary Office, Ministry of Agriculture and Livestock, P.O. Box 80285, Kabwe, Zambia.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Mainda
authors.affiliation: Genetics and Genomics, The Roslin Institute and The Royal (Dick) School of Veterinary Sciences, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Bessell
authors.forename: Paul B
authors.initials: PB
authors.lastname: Bessell
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, Zambia.
authors.forename: John B
authors.initials: JB
authors.lastname: Muma
authors.affiliation: Divisions of Immunity and Infection, Veterinary Clinical Sciences.
authors.forename: Sean P
authors.initials: SP
authors.lastname: McAteer
authors.affiliation: Centre for Immunity, Infection and Evolution, Kings Buildings, University of Edinburgh, Charlotte Auerbach Road, Edinburgh, EH9 3FL, United Kingdom.
authors.forename: Margo E
authors.initials: ME
authors.lastname: Chase-Topping
authors.affiliation: School of Veterinary Medicine, Veterinary Science Centre, Belfield, Dublin 4, Eire.
authors.forename: James
authors.initials: J
authors.lastname: Gibbons
authors.affiliation: Divisions of Immunity and Infection, Veterinary Clinical Sciences.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.affiliation: Divisions of Immunity and Infection, Veterinary Clinical Sciences.
authors.forename: David L
authors.initials: DL
authors.lastname: Gally
authors.affiliation: 1] Divisions of Immunity and Infection, Veterinary Clinical Sciences [2] Genetics and Genomics, The Roslin Institute and The Royal (Dick) School of Veterinary Sciences, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, United Kingdom.
authors.forename: Barend M
authors.initials: BM
authors.lastname: deC Bronsvoort
mesh_terms: D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle / Q000382:microbiology*; D003612:Dairying / Q000379:methods*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D015024:Zambia
chemical_list: D000900:Anti-Bacterial Agents