title: Transplanted microvascular endothelial cells promote oligodendrocyte precursor cell survival in ischemic demyelinating lesions.
abstract: We previously showed that transplantation of brain microvascular endothelial cells (MVECs) greatly stimulated remyelination in the white matter infarct of the internal capsule (IC) induced by endothelin-1 injection and improved the behavioral outcome. In the present study, we examined the effect of MVEC transplantation on the infarct volume using intermittent magnetic resonance image and on the behavior of oligodendrocyte lineage cells histochemically. Our results in vivo show that MVEC transplantation reduced the infarct volume in IC and apoptotic death of oligodendrocyte precursor cells (OPCs). These results indicate that MVECs have a survival effect on OPCs, and this effect might contribute to the recovery of the white matter infarct. The conditioned-medium from cultured MVECs reduced apoptosis of cultured OPCs, while the conditioned medium from cultured fibroblasts did not show such effect. These results suggest a possibility that transplanted MVECs increased the number of OPCs through the release of humoral factors that prevent their apoptotic death. Identification of such humoral factors may lead to the new therapeutic strategy against ischemic demyelinating diseases.
authors.affiliation: Department of Neurosurgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Keiya
authors.initials: K
authors.lastname: Iijima
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurachi
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Shibasaki
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Naruse
authors.affiliation: Department of Neurosurgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Sandra
authors.initials: S
authors.lastname: Puentes
authors.affiliation: Department of Neurosurgery, Tokyo University Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Imai
authors.affiliation: Department of Neurosurgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Yoshimoto
authors.affiliation: Department of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Mikuni
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ishizaki
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000628:therapy*; D002470:Cell Survival / Q000502:physiology; D003711:Demyelinating Diseases / Q000473:pathology / Q000628:therapy*; D042783:Endothelial Cells / Q000637:transplantation*; D008297:Male; D055806:Microvessels / Q000166:cytology / Q000637:transplantation*; D009836:Oligodendroglia / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000502:physiology*
keywords: cell survival; cerebral microvascular endothelial cells; cerebral small vessel disease; oligodendrocyte; precursor cell; remyelination