title: Forkhead box protein O1 mediates apoptosis in a cancer cervical cell line treated with the antitumor agent tumor necrosis factor-α.
abstract: Tumor necrosis factor-α (TNF-α) is an important pro-apoptotic cytokine, which performs a broad range of immune and inflammatory functions in several vital processes. TNF-α-induced apoptosis has been confirmed, however, relatively little is known regarding the role of forkhead box class-O 1 (FOXO1) in mediating TNF-α-induced apoptosis in cervical cancer. In our study, we used the well-characterized cervical cancer cell line C-33A to investigate the role of FOXO1. The results showed that the antitumor agent TNF-α increased the expression level of FOXO1 (P < 0.05) and enhanced its transcriptional activity (P < 0.05). Furthermore, knockdown of FOXO1 repressed TNF-α-induced apoptosis and caspase-3, 8, and 9 expressions (P < 0.05). Collectively, these findings suggest that TNF-α upregulated the transcriptional factor FOXO1, leading to an increased expression of apoptotic gene, which leads to an increase in apoptosis.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.
authors.forename: L
authors.initials: L
authors.lastname: Gui
authors.affiliation: College of Basic Medical Sciences, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Basic Medical Sciences, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Basic Medical Sciences, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China liqingwangysu@aliyun.com.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D020169:Caspases