title: Role of mTOR1 and mTOR2 complexes in MEG-01 cell physiology.
abstract: The function of the mammalian target of rapamycin (mTOR) is upregulated in response to cell stimulation with growing and differentiating factors. Active mTOR controls cell proliferation, differentiation and death. Since mTOR associates with different proteins to form two functional macromolecular complexes, we aimed to investigate the role of the mTOR1 and mTOR2 complexes in MEG-01 cell physiology in response to thrombopoietin (TPO). By using mTOR antagonists and overexpressing FKBP38, we have explored the role of both mTOR complexes in proliferation, apoptosis, maturation-like mechanisms, endoplasmic reticulum-stress and the intracellular location of both active mTOR complexes during MEG-01 cell stimulation with TPO. The results demonstrate that mTOR1 and mTOR2 complexes play different roles in the physiology of MEG-01 cells and in the maturation-like mechanisms; hence, these findings might help to understand the mechanism underlying generation of platelets.
authors.forename: Esther
authors.initials: E
authors.lastname: López
authors.forename: Alejandro
authors.initials: A
authors.lastname: Berna-Erro
authors.forename: Javier J
authors.initials: JJ
authors.lastname: López
authors.forename: María P
authors.initials: MP
authors.lastname: Granados
authors.forename: Nuria
authors.initials: N
authors.lastname: Bermejo
authors.forename: Jose M
authors.initials: JM
authors.lastname: Brull
authors.forename: Ginés M
authors.initials: GM
authors.lastname: Salido
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rosado
authors.affiliation: Pedro Cosme Redondo Liberal, PhD, Department of Physiology, University of Extremadura, Avd. de la Universidad s/n PD. 10003 , Cáceres, Spain, Tel.: +34 927 25 71 06 ext.: 5 15 22, Fax: +34 927 25 71 10, E-mail: pcr@unex.es.
authors.forename: Pedro C
authors.initials: PC
authors.lastname: Redondo
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D000076222:Mechanistic Target of Rapamycin Complex 1; D000076225:Mechanistic Target of Rapamycin Complex 2; D055016:Megakaryocyte Progenitor Cells / Q000187:drug effects / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism*; D009287:Naphthyridines / Q000494:pharmacology; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D022061:Tacrolimus Binding Protein 1A / Q000235:genetics / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000378:metabolism; D013926:Thrombopoietin / Q000378:metabolism; D019076:Transgenes / Q000235:genetics
chemical_list: C553294:1-(4-(4-propionylpiperazin-1-yl)-3-(trifluoromethyl)phenyl)-9-(quinolin-3-yl)benzo(h)(1,6)naphthyridin-2(1H)-one; C471205:FKBP8 protein, human; D046912:Multiprotein Complexes; D009287:Naphthyridines; D013926:Thrombopoietin; D000076222:Mechanistic Target of Rapamycin Complex 1; D000076225:Mechanistic Target of Rapamycin Complex 2; D058570:TOR Serine-Threonine Kinases; D022061:Tacrolimus Binding Protein 1A; D022021:Tacrolimus Binding Proteins; D020123:Sirolimus
keywords: MEG-01 cells; apoptosis; calcium homeostasis; mTOR; rapamycin; raptor; rictor; torin1