title: 7α-Hydroxypregnenolone, a key neuronal modulator of locomotion, stimulates upstream migration by means of the dopaminergic system in salmon.
abstract: Salmon migrate upstream against an opposing current in their natal river. However, the molecular mechanisms that stimulate upstream migratory behavior are poorly understood. Here, we show that 7α-hydroxypregnenolone (7α-OH PREG), a newly identified neuronal modulator of locomotion, acts as a key factor for upstream migration in salmon. We first identified 7α-OH PREG and cytochrome P450 7α-hydroxylase (P4507α), a steroidogenic enzyme producing 7α-OH PREG, in the salmon brain and then found that 7α-OH PREG synthesis in the brain increases during upstream migration. Subsequently, we demonstrated that 7α-OH PREG increases upstream migratory behavior of salmon. We further found that 7α-OH PREG acts on dopamine neurons in the magnocellular preoptic nucleus during upstream migration. Thus, 7α-OH PREG stimulates upstream migratory behavior through the dopaminergic system in salmon. These findings provide new insights into the molecular mechanisms of fish upstream migration.
authors.affiliation: 1] Department of Biology and Center for Medical Life Science, Waseda University, Tokyo, Japan [2] Department of Biology, Tokyo Gakugei University, Tokyo, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Haraguchi
authors.affiliation: 1] Field Science Center for Northern Biosphere, Hokkaido University, Hokkaido, Japan [2] Current address: Demonstration Laboratory, Marine Ecology Research Institute, Niigata, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Biology and Center for Medical Life Science, Waseda University, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Biology and Center for Medical Life Science, Waseda University, Tokyo, Japan.
authors.forename: Joon
authors.initials: J
authors.lastname: Hyung Chang
authors.affiliation: Department of Biology and Center for Medical Life Science, Waseda University, Tokyo, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Koyama
authors.affiliation: Department of Biology and Center for Medical Life Science, Waseda University, Tokyo, Japan.
authors.forename: Miku
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Biology, Tokyo Gakugei University, Tokyo, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Mita
authors.affiliation: Field Science Center for Northern Biosphere, Hokkaido University, Hokkaido, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
authors.affiliation: Department of Biology and Center for Medical Life Science, Waseda University, Tokyo, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Tsutsui
mesh_terms: D006907:17-alpha-Hydroxypregnenolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005260:Female; D008124:Locomotion / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D012474:Salmon / Q000502:physiology*
chemical_list: C495856:7-hydroxypregnenolone; D000697:Central Nervous System Stimulants; D006907:17-alpha-Hydroxypregnenolone; D004298:Dopamine