title: Vincristine and temozolomide combined chemotherapy for the treatment of glioma: a comparison of solid lipid nanoparticles and nanostructured lipid carriers for dual drugs delivery.
abstract: BACKGROUND
  Glioma is a common malignant brain tumor originating in the central nervous system. Efficient delivery of therapeutic agents to the cells and tissues is a difficult challenge. Co-delivery of anticancer drugs into the cancer cells or tissues by multifunctional nanocarriers may provide a new paradigm in cancer treatment.
  
  
  OBJECTIVE
  In this study, solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) were constructed for co-delivery of vincristine (VCR) and temozolomide (TMZ) to develop the synergetic therapeutic action of the two drugs. The antitumor effects of these two systems were compared to provide a better choice for gliomatosis cerebri treatment.
  
  
  METHODS
  VCR- and TMZ-loaded SLNs (VT-SLNs) and NLCs (VT-NLCs) were formulated. Their particle size, zeta potential, drug encapsulation efficiency (EE) and drug loading capacity were evaluated. The single TMZ-loaded SLNs and NLCs were also prepared as contrast. Anti-tumor efficacies of the two kinds of carriers were evaluated on U87 malignant glioma cells and mice bearing malignant glioma model.
  
  
  RESULTS
  Significantly better glioma inhibition was observed on NLCs formulations than SLNs, and dual drugs displayed the highest antitumor efficacy in vivo and in vitro than all the other formulations used.
  
  
  CONCLUSIONS
  VT-NLCs can deliver VCR and TMZ into U87MG cells more efficiently, and inhibition efficacy is higher than VT-SLNs. This dual drugs-loaded NLCs could be an outstanding drug delivery system to achieve excellent therapeutic efficiency for the treatment of malignant gliomatosis cerebri.
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Miaojing
authors.initials: M
authors.lastname: Wu
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Yanghua
authors.initials: Y
authors.lastname: Fan
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Lv
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xiao
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Ye
authors.affiliation: a Department of Neurosurgery , Second Affiliated Hospital, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Xingen
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D004359:Drug Therapy, Combination / Q000379:methods; D005910:Glioma / Q000188:drug therapy*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D000077204:Temozolomide; D014750:Vincristine / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; D014750:Vincristine; D003606:Dacarbazine; D000077204:Temozolomide
keywords: Co-delivery; combined chemotherapy; glioblastoma multiforme; nanostructured lipid carriers; solid lipid nanoparticles