title: Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology.
abstract: Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of ob-served alleles per locus is 6.6 (ranging from 2 to 17). Observed and expected heterozygosity values ranged from 0 to 0.958 and from 0.508 to 0.940, respectively. Not a single locus showed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was observed among any pairwise loci. These highly informative microsatellite markers will be useful for genetic diversity and population structure analyses of A. schrenckii and other species of this genus.
authors.affiliation: Guangdong Entomological Institute, Guangzhou, China.
authors.forename: L M
authors.initials: LM
authors.lastname: Li
authors.affiliation: College of Ecology, Lishui University, Lishui, China.
authors.forename: L
authors.initials: L
authors.lastname: Wei
authors.affiliation: Guangdong Entomological Institute, Guangzhou, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Jiang
authors.affiliation: College of Animal Science of Inner Mongolia Agriculture University, Hohhot, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangdong Entomological Institute, Guangzhou, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Guangdong Entomological Institute, Guangzhou, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Yuan
authors.affiliation: Guangdong Entomological Institute, Guangzhou, China chenjp@gdei.gd.cn.
authors.forename: J P
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000818:Animals; D005399:Fishes / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic