title: Expressions of matrix metalloproteinases-1 and -9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine.
abstract: OBJECTIVE
  To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy.
  
  
  METHODS
  The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 µL of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed.
  
  
  RESULTS
  The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05).
  
  
  CONCLUSIONS
  The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy.
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: Miguel Ladino
authors.initials: ML
authors.lastname: Silva
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary and Agricultural Sciences, Universidade Federal do Mato Grosso, Cuiabá, MT, Brazil.
authors.forename: Alexandre Pinto
authors.initials: AP
authors.lastname: Ribeiro
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: Germana Alegro
authors.initials: GA
authors.lastname: Silva
authors.affiliation: College of Veterinary Medicine and Animal Science, University of Tolima, Ibague, Colombia.
authors.forename: Irma Ximena Barbosa
authors.initials: IX
authors.lastname: Sanchez
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Renzo
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Uscategui
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: Tiago Barbalho
authors.initials: TB
authors.lastname: Lima
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: Marcela
authors.initials: M
authors.lastname: Aldrovani
authors.affiliation: Department of Medicine and Surgery, College of Agricultural and Veterinarian Sciences, Universidade de São Paulo, Jaboticabal, SP, Brazil.
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Laus
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D003319:Corneal Stroma / Q000378:metabolism / Q000473:pathology; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects* / Q000378:metabolism; D023421:Models, Animal; D009266:Nalbuphine / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D011957:Receptors, Opioid / Q000187:drug effects* / Q000378:metabolism; D054140:Refractive Surgical Procedures / Q000379:methods
chemical_list: D000701:Analgesics, Opioid; D011957:Receptors, Opioid; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1; D009266:Nalbuphine