title: Directional migration of cancer cells induced by a blue light intensity gradient.
abstract: We used a spatial light modulator to project an optical micropattern of 473 nm light with a quartic intensity gradient on a single lung cancer cell. We observed that the intracellular amounts of reactive oxygen species (ROS) of the cancer cells were proportional to the intensity of the blue light, and the blue light intensity gradients could drive directional cell migration. This optically induced directional cell migration was inhibited by a ROS scavenger in the culture medium in a dose-dependent manner. In contrast, the ROS levels in fibroblasts were saturated by the blue light at low intensity and therefore the fibroblasts did not exhibit directional migration in the intensity gradient.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei 11221, Taiwan.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Lan
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei 11221, Taiwan.
authors.forename: Eugene Youjhen
authors.initials: EY
authors.lastname: Lu
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Huei-Jyuan
authors.initials: HJ
authors.lastname: Pan
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei 11221, Taiwan ; Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Chau-Hwang
authors.initials: CH
authors.lastname: Lee
keywords: (170.0170) Medical optics and biotechnology; (170.4520) Optical confinement and manipulation