title: Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration.
abstract: Bacterial cellulose (BC) is an alternative nanostructured biomaterial to be utilized for a wide range of biomedical applications. Because of its low bioactivity, which restricted its practical application, collagen and collagen hydrolysate were usually composited into BC. It is necessary to develop a new method to generate covalent bonds between collagen and cellulose to improve the immobilization of collagen on BC. This study describes a facile dialdehyde BC/collagen peptide nanocomposite. BC was oxidized into dialdehyde bacterial cellulose (DBC) by regioselective oxidation, and then composited with collagen peptide (Col-p) via covalent bonds to form Schiff's base type compounds, which was demonstrated by the results of microstructures, contact angle, Col-p content, and peptide-binding ratio. The peptide-binding ratio was further affected by the degree of oxidation, pH value, and zeta potential. In vitro desorption measurement of Col-p suggested a controlled release mechanism of the nanocomposite. Cell tests indicated that the prepared DBC/Col-p composite was bioactive and suitable for cell adhesion and attachment. This work demonstrates that the DBC/Col-p composite is a promising material for tissue engineering and regeneration.
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wen
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Soochow, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China.
authors.forename: Lu-Ning
authors.initials: LN
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Haoye
authors.initials: H
authors.lastname: Meng
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; D002482:Cellulose; D003094:Collagen; C058684:2,3-dialdehydocellulose
keywords: bacterial cellulose; collagen peptide; composite materials; cytoactivity; dialdehyde cellulose