title: Novel source of human hematopoietic stem cells from peritoneal dialysis effluents.
abstract: Hematopoietic stem cells (HSCs) hold great promise for the treatment of various diseases and blood disorders. However, limited availability of these cells has hampered their applications in clinical and biological research. Here we have identified a new source of autologous human HSCs in peritoneal dialysis (PD) effluents from patients with end stage renal diseases (ESRDs). Cells isolated from PD effluents contain a Lin-/CD34+/CD38-/CD90+ sub-population and can repopulate NOD/SCID/gamma-/- mice in serial transplantation. Differing from cord blood HSCs, PD-derived HSCs have high tendencies to repopulate peritoneal cavity and spleen with myeloid cells and B lymphocytes. Repopulating HSCs also reside in peritoneal cavities in mice. The isolation of HSCs from peritoneal cavities provides a novel and promising source of autologous and functional HSCs for stem cell research and possible clinical use.
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, PO Box 19138, Arlington, TX 76019, United States.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Shen
authors.affiliation: Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, United States.
authors.forename: Junke
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Division of Nephrology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390, United States.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Saxena
authors.affiliation: Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, United States. Electronic address: Alec.Zhang@UTSouthwestern.edu.
authors.forename: ChengCheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, PO Box 19138, Arlington, TX 76019, United States; Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: ltang@uta.edu.
authors.forename: Liping
authors.initials: L
authors.lastname: Tang
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D010530:Peritoneal Dialysis; D018800:Thy-1 Antigens / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34; D018800:Thy-1 Antigens; D051997:ADP-ribosyl Cyclase 1
keywords: Hematopoietic stem cells; Peritoneal dialysis