title: Application and optimization of microwave-assisted extraction and dispersive liquid-liquid microextraction followed by high-performance liquid chromatography for sensitive determination of polyamines in turkey breast meat samples.
abstract: A novel method based on microwave-assisted extraction and dispersive liquid-liquid microextraction (MAE-DLLME) followed by high-performance liquid chromatography (HPLC) was developed for the determination of three polyamines from turkey breast meat samples. Response surface methodology (RSM) based on central composite design (CCD) was used to optimize the effective factors in DLLME process. The optimum microextraction efficiency was obtained under optimized conditions. The calibration graphs of the proposed method were linear in the range of 20-200 ng g(-1), with the coefficient determination (R(2)) higher than 0.9914. The relative standard deviations were 6.72-7.30% (n = 7). The limits of detection were in the range of 0.8-1.4 ng g(-1). The recoveries of these compounds in spiked turkey breast meat samples were from 95% to 105%. The increased sensitivity in using the MAE-DLLME-HPLC-UV has been demonstrated. Compared with previous methods, the proposed method is an accurate, rapid and reliable sample-pretreatment method.
authors.affiliation: Department of Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Moein
authors.initials: M
authors.lastname: Bashiry
authors.affiliation: Department of Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: ab.mohammadi@sbmu.ac.ir.
authors.forename: Abdorreza
authors.initials: A
authors.lastname: Mohammadi
authors.affiliation: Department of Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: hedayat@sbmu.ac.ir.
authors.forename: Hedayat
authors.initials: H
authors.lastname: Hosseini
authors.affiliation: Department of Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Chemistry, Faculty of Science, Alzahra University, Tehran, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Kamankesh
authors.affiliation: Department of Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Aeenehvand
authors.affiliation: Department of Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Zaniar
authors.initials: Z
authors.lastname: Mohammadi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059627:Liquid Phase Microextraction / Q000379:methods*; D008460:Meat / Q000032:analysis*; D008872:Microwaves / Q000627:therapeutic use*; D011073:Polyamines / Q000032:analysis / Q000737:chemistry*; D014421:Turkey
chemical_list: D011073:Polyamines
keywords: Dispersive liquid–liquid microextraction; High-performance liquid chromatography; Polyamines; Response surface methodology; Turkey breast meat