title: Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs.
abstract: OBJECTIVE
  Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals and is the most damaging disease of livestock worldwide, leading to great economic losses. The aim of this research was the inactivation of FMDV type O/IRN/1/2007 to produce a gamma ray-irradiated (GRI) vaccine in order to immunize mice and guinea pigs.
  
  
  METHODS
  In this research, the Iranian isolated FMDV type O/IRN/1/2007 was irradiated by gamma ray to prepare an inactivated whole virus antigen and formulated as a GRI vaccine with unaltered antigenic characteristics. Immune responses against this vaccine were evaluated on mice and guinea pigs.
  
  
  RESULTS
  The comparison of the immune responses between the GRI vaccine and conventional vaccine did not show any significant difference in neutralizing antibody titer, memory spleen T lymphocytes or IFN-γ, IL-4, IL-2 and IL-10 concentrations (p > 0.05). In contrast, there were significant differences in all of the evaluated immune factors between the two vaccinated groups of mice and negative control mice (p < 0.05). The protective dose 50 for the conventional and GRI vaccines obtained were 6.28 and 7.07, respectively, which indicated the high potency of both vaccines.
  
  
  CONCLUSIONS
  GRI vaccine is suitable for both routine vaccination and control of FMDV in emergency outbreaks.
authors.affiliation: Nuclear Science and Technology Research Institute, Karaj, Tehran, Iran.
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Motamedi-Sedeh
authors.forename: Hoorieh
authors.initials: H
authors.lastname: Soleimanjahi
authors.forename: Amir Reza
authors.initials: AR
authors.lastname: Jalilian
authors.forename: Homayoon
authors.initials: H
authors.lastname: Mahravani
authors.forename: Kamalodin
authors.initials: K
authors.lastname: Shafaee
authors.forename: Masood
authors.initials: M
authors.lastname: Sotoodeh
authors.forename: Hamdolah
authors.initials: H
authors.lastname: Taherkarami
authors.forename: Faramarz
authors.initials: F
authors.lastname: Jairani
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins; D016207:Cytokines / Q000276:immunology; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology* / Q000528:radiation effects; D005720:Gamma Rays; D006168:Guinea Pigs; D007156:Immunologic Memory; D007492:Iran; D008213:Lymphocyte Activation; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes; D064166:Vaccine Potency; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D016207:Cytokines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines