title: Black Soybean Seed Coat Extract Prevents Hydrogen Peroxide-Mediated Cell Death via Extracellular Signal-Related Kinase Signalling in HepG2 Cells.
abstract: Oxidative stress reduces cell viability and contributes to disease processes. Flavonoids including anthocyanins and proanthocyanidins reportedly induce intracellular antioxidant defence systems. Thus, in this study, we examined the antioxidant effects of a commercial extract from black soybean seed coats (BE), which are rich in anthocyanin and proanthocyanidin, and investigated the associated intracellular mechanisms in HepG2 cells. HepG2 cells treated with hydrogen peroxide (HPO) showed 60% viability, whereas pretreatment with BE-containing media for 2 h ameliorated HPO-mediated cell death by up to 90%. Pretreatment with BE for 2 h partially blocked HPO-mediated activation of ERK in HepG2 cells, and that for 1 h led to a 20% increase in intracellular total protein phosphatase (PP) activity, which is known to deactivate protein kinases. These results indicate that BE prevents HPO-mediated cell damage by inhibiting ERK signalling, potentially via PPs.
authors.affiliation: Crop and Agribusiness Research Division, NARO Kyushu Okinawa Agricultural Research Center.
authors.forename: Naoto
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Oki
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sasaki
authors.forename: Ikuo
authors.initials: I
authors.lastname: Suda
authors.forename: Shigenori
authors.initials: S
authors.lastname: Okuno
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts; D044945:Proanthocyanidins; D006861:Hydrogen Peroxide; D048049:Extracellular Signal-Regulated MAP Kinases; D010749:Phosphoprotein Phosphatases