title: Modulating the SDF-1/CXCL12-induced cancer cell growth and adhesion by sulfated K5 polysaccharides in vitro.
abstract: Stromal cell-derived factor-1 (SDF-1)/chemokine (CXC motif) ligand 12 (CXCL12) is involved in the process of tumor progression. Sulfated K5 polysaccharides have shown anti-cancer activity by acting on multiple targets, though it remains unclear whether sulfated K5 polysaccharides would disrupt SDF-1/CXCL12-stimulated cancer biology. This study aimed to investigate the effects of sulfated K5 polysaccharides on cell growth, adhesion in murine B16 melanoma cells and the underlying mechanism by targeting SDF-1/CXCL12. Results indicated that K5-NS,OS inhibited the proliferation of B16 melanoma cells, induced the cell cycle arrest mainly at the G0/G1 phase, and suppressed cancer cell proliferation or adhesion induced by SDF-1/CXCL12. It was possible that K5-NS,OS appeared to interact with CXCL12 and block the subsequent biological functions. This work suggests that the existence of O- and N-sulfate groups is more effective in targeting CXCL12 and exhibiting anti-cancer activity.
authors.affiliation: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Teng
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Fu
authors.affiliation: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Jingxiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: jhchenwhut@126.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D016667:Bacterial Capsules; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D054377:Chemokine CXCL12 / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology* / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C050673:capsular polysaccharide K5
keywords: Cell adhesion; Cell growth; Escherichia coli K5 capsular polysaccharide; SDF-1/CXCL12; Sulfation