title: Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls.
abstract: The derivation of two sensitive monoclonal antibodies directed to heteroxylan cell wall polysaccharide preparations has allowed the identification of potential inter-linkages between xylan and pectin in potato tuber cell walls and also between xylan and arabinogalactan-proteins in oat grain cell walls. Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links.
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Valérie
authors.initials: V
authors.lastname: Cornuault
authors.affiliation: UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Buffetto
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark.
authors.forename: Maja G
authors.initials: MG
authors.lastname: Rydahl
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Marcus
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Torode
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Jie
authors.initials: J
authors.lastname: Xue
authors.affiliation: UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France.
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Crépeau
authors.affiliation: Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK.
authors.forename: Nuno
authors.initials: N
authors.lastname: Faria-Blanc
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark.
authors.forename: William G T
authors.initials: WG
authors.lastname: Willats
authors.affiliation: Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Dupree
authors.affiliation: UR1268 Biopolymères, Interactions et Assemblages, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627, 44316, Nantes, France.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Ralet
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK. j.p.knox@leeds.ac.uk.
authors.forename: J Paul
authors.identifier: http://orcid.org/0000-0002-9231-6891
authors.initials: JP
authors.lastname: Knox
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011134:Polysaccharides; D014990:Xylans; C042491:rhamnogalacturonan I; C038910:glucuronoxylan; D010368:Pectins
keywords: Arabinogalactan-proteins; Cell wall; Glucuronoxylan; Pectin; Polysaccharides; Rhamnogalacturonan-I