title: Human papillomavirus infection and p16 overexpression in oropharyngeal squamous cell carcinoma: a case series from 2010 to 2014.
abstract: OBJECTIVE
  Human papillomavirus (HPV) associated oropharyngeal squamous cell carcinomas (OPSCC) show better prognosis and response to therapy. We evaluated HPV-DNA prevalence, p16 overexpression and HPV-attributable fraction in recent consecutive OPSCCs.
  
  
  METHODS
  140 OPSCCs were analyzed for HPV-DNA using the INNO-LiPA assay and p16 overexpression using CINtec® Histology kit.
  
  
  RESULTS
  HPV-DNA prevalence was 40.7%, and was significantly higher in basaloid and nonkeratinizing SCCs, but similar in tonsillar and base of tongue SCCs. Moreover, 98.0% of the HPV-positive OPSCCs overexpressed p16, compared with 12.3% of the HPV-negative cases (p < 0.0001). Based on HPV-DNA and p16 double positivity, 39.8% of the cases were HPV related.
  
  
  CONCLUSIONS
  The high estimate of the HPV-attributable fraction suggests that a substantial proportion of OPSCC patients may be managed less intensively.
authors.affiliation: Sexually Transmitted Infection (STI) Unit, San Gallicano Dermatologic Institute, IRCCS, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Donà
authors.affiliation: Otolaryngology Head Neck Surgery Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spriano
authors.affiliation: Otolaryngology Head Neck Surgery Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pichi
authors.affiliation: Pathology Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144, Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rollo
authors.affiliation: Pathology Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144, Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Laquintana
authors.affiliation: Pathology Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144, Rome, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Covello
authors.affiliation: Otolaryngology Head Neck Surgery Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Raul
authors.initials: R
authors.lastname: Pellini
authors.affiliation: Sexually Transmitted Infection (STI) Unit, San Gallicano Dermatologic Institute, IRCCS, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Giuliani
authors.affiliation: Pathology Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144, Rome, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Pescarmona
authors.affiliation: Pathology Department, Regina Elena National Cancer Institute, IRCCS, Via Elio Chianesi 53, 00144, Rome, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Benevolo
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015870:Gene Expression; D019942:Genes, p16; D005838:Genotype; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000209:etiology / Q000235:genetics / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D011379:Prognosis; D013997:Time Factors
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral
keywords: head and neck neoplasms; human papillomavirus; oropharyngeal neoplasms; p16INK4 protein; prevalence