title: Expression Analysis of mir-21 and mir-221 in Cancerous Tissues from Iranian Patients with Gastric Cancer.
abstract: BACKGROUND
  Early detection is a key to survival for gastric cancer. Molecular markers such as miRNA (microRNA) can have great importance in the early diagnosis of gastric cancer. Expression of miR-21 and miR-221 are deregulated in many types of human cancers. This study aimed to investigate the differences in miRNA expression patterns within the Iranian population.
  
  
  METHODS
  Total RNA was extracted from gastric cancer tissues and adjacent non-cancerous tissues from 32 patients. Expression levels of miR-21 and miR-221 were detected by Real time RT-PCR using a specific primer, with 5s rRNA as the internal reference gene.
  
  
  RESULTS
  Our data showed that the expression levels of miR-21 and miR-221 in gastric cancer samples were significantly higher than in paired non-cancerous samples (P value less than 0.05). The receiver operating characteristic (ROC) analyses yielded the area under the curve (AUC) values of 80.30 for miR-21 and 93.30 for miR-221, and combined ROC analysis revealed the highest AUC value of 96.90 in discriminating GC patients from healthy controls.
  
  
  CONCLUSIONS
  It seems that miR-21 and miR-221 expression pattern in Iranian patients with gastric cancer are similar to any other population. Considering the increased expression level of two miRNAs in cancerous tissue compared to normal tissue as well as the area under ROC curve, miR-21 and miR-221 can be used for early detection of gastric cancer.
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|Dept. of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Hosein
authors.initials: H
authors.lastname: Effatpanah
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|Dept. of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Yadegarazari
authors.affiliation: Social Determinant of Health Research Center and Dept. of Biostatistics and Epidemiology, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Manoochehr
authors.initials: M
authors.lastname: Karami
authors.affiliation: Dept. of Gastrointestinal Disease, Beheshti Hospital of Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Majlesi
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|Dept. of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Shabab
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|Dept. of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007492:Iran; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C524940:MIRN21 microRNA, human; C525709:MIR221, human
keywords: MicroRNAs; Stomach neoplasms; Tumor markers