title: Caspases and Thrombin Activity Regulation by Specific Serpin Inhibitors in Bovine Skeletal Muscle.
abstract: In living cells, after activation, protein inhibitors constitute the last step of proteases activity regulation. This review intends to provide original information about a group of bovine muscle serine proteases inhibitors belonging to the Serpin superfamily and characterized at the gene and protein level. This report is the only one and the first to provide much information on this group of proteases inhibitors of the serpin type and their potential biological functions. Amongst the eight genes identified in bovine, three serpins were purified from the muscle tissue and characterized. These are two members of the bovSERPINA3 family, i.e., bovSERPINA3-1 and A3-3, and the last one is antithrombin III (AT-III or BovSERPINC1). BovSERPINA3 family comprises at least eight protein members encoded by different genes mapped on chromosome 7q23-q26 cluster. BovSERPINA3-1 and A3-3 were shown to locate within muscle cells and are cross-class inhibitors strongly active against trypsin as well as against human initiator and effector caspases 8 and 3. They constitute a key apoptosis control in mammals. They were thus expressed in proliferating and confluent myoblasts phases where cells must be alive but not in myotubes. Antithrombin III inhibits trypsin and, in a heparin dependent manner, thrombin. AT-III and its mRNA were expressed in muscle cells and in differentiating primary myoblasts in culture.
authors.affiliation: Maquav team, Bioqual Laboratory, INATAA (Institut de la Nutrition, de l'Alimentation et des Technologies Agro-Alimentaires), Université Frères Mentouri Constantine 1, Route de Ain El-Bey, 25000, Constantine, Algeria, gmber2001@yahoo.fr.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Gagaoua
authors.forename: Kahina
authors.initials: K
authors.lastname: Hafid
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Boudida
authors.forename: Samira
authors.initials: S
authors.lastname: Becila
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ouali
authors.forename: Brigitte
authors.initials: B
authors.lastname: Picard
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Boudjellal
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Sentandreu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020169:Caspases / Q000378:metabolism*; D002417:Cattle; D007700:Kinetics; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000378:metabolism*; D015843:Serpins / Q000737:chemistry / Q000378:metabolism*; D013917:Thrombin / Q000378:metabolism*
chemical_list: D015842:Serine Proteinase Inhibitors; D015843:Serpins; D013917:Thrombin; D020169:Caspases