title: Propofol suppresses proliferation and invasion of pancreatic cancer cells by upregulating microRNA-133a expression.
abstract: Propofol is a commonly used intravenous anesthetic. We evaluated its effects on the behavior of human pancreatic cancer cells and the underlying molecular mechanisms. The effects of propofol on Panc-1 cell proliferation, apoptosis, and invasion were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, caspase-3 activity measurement, and Matrigel invasion assay. Quantitative polymerase chain reaction (qPCR) was used to assess microRNA-133a (miR-133a) expression. Anti-miR-133a was transfected into Panc-1 cells to assess the role of miR-133a in propofol-induced antitumor activity. Propofol significantly inhibited Panc-1 cell proliferation and invasion, and promoted apoptosis. Propofol also efficiently elevated miR-133a expression. Moreover, transfection of anti-miR-133a reversed the effects of propofol on the biological behavior of Panc-1 cells. Propofol can effectively inhibit proliferation and invasion, and induce apoptosis of pancreatic cancer cells, at least partly through the upregulation of miR-133a expression.
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, Jinshui District, Zhengzhou, China.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Gong
authors.affiliation: Department of Medical Ultrasonics, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan, China.
authors.forename: F
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, Jinshui District, Zhengzhou, China.
authors.forename: D J
authors.initials: DJ
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, Jinshui District, Zhengzhou, China manudongtl@163.com.
authors.forename: T L
authors.initials: TL
authors.lastname: Dong
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015742:Propofol / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: C548234:MIRN133 microRNA, human; D035683:MicroRNAs; D015742:Propofol