title: Improved separation and analysis of glycolipids by Iatroscan thin-layer chromatography-flame ionization detection.
abstract: We demonstrate improved power of Iatroscan thin layer chromatography/flame ionization detection (TLC-FID) technique for analysis of complex marine lipid mixture by developing protocol for the separation and analysis of glycolipids including sulfoquinovosyldiacylglycerols (SQDG), monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglycerols (DGDG). We have modified the common protocol used so far for the analysis of lipid classes by replacing the elution step which uses pure acetone for the elution of acetone mobile polar lipids, with the elution step containing chloroform-acetone (72:28, v:v) for separation of MGDG and DGDG. To separate SQDG from the complex lipid matrix we introduced solvent mixture acetone-chloroform-methanol-formic acid (33:33:33:0.6, v:v:v:v). Quantification of glycolipid classes was performed after calibration with glycolipid standards for the masses between 0.2 and 2.7-5.0μg. With this new protocol we have successfully separated three glycolipids from the complex particulate lipid mixture of the seawater samples. Such an approach extends the power of existing protocol for the analysis of lipids which altogether ensure detection and quantification of 18 lipid classes what was demonstrated on seawater samples. This enables to gain a very broad system overview of the particularly complex environments as are seas, oceans and freshwaters.
authors.affiliation: Division for Marine and Environmental Research, Ruđer Bošković Institute, POB 180, HR-10002 Zagreb, Croatia. Electronic address: gaspar@irb.hr.
authors.forename: Blaženka
authors.initials: B
authors.lastname: Gašparović
authors.affiliation: Division of Physical Chemistry; Ruđer Bošković Institute, POB 180, HR-10002 Zagreb, Croatia.
authors.forename: Snježana P
authors.initials: SP
authors.lastname: Kazazić
authors.affiliation: Division for Marine and Environmental Research, Ruđer Bošković Institute, POB 180, HR-10002 Zagreb, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Cvitešić
authors.affiliation: Division for Marine and Environmental Research, Ruđer Bošković Institute, POB 180, HR-10002 Zagreb, Croatia.
authors.forename: Abra
authors.initials: A
authors.lastname: Penezić
authors.affiliation: Division for Marine and Environmental Research, Ruđer Bošković Institute, POB 180, HR-10002 Zagreb, Croatia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Frka
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods; D005410:Flame Ionization; D038983:Galactolipids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis*; D012623:Seawater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D038983:Galactolipids; D006017:Glycolipids; D014874:Water Pollutants, Chemical; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; C013694:sulfoquinovosyl diglyceride
keywords: Digalactosyldiacylglycerol (DGDG); Iatroscan thin-layer chromatography; Monogalactosyldiacylglycerol (MGDG); Sulfoquinovosyldiacylglycerol (SQDG)