title: Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis.
abstract: Speradine A is a derivative of cyclopiazonic acid (CPA) found in culture of an Aspergillus tamarii isolate. Heterologous expression of a predicted methyltransferase gene, cpaM, in the cpa biosynthesis gene cluster of A. tamarii resulted in the speradine A production in a 2-oxoCPA producing A. oryzae strain, indicating cpaM is involved in the speradine A biosynthesis.
authors.affiliation: a Faculty of Applied Bioscience, Department of Fermentation Science and Technology , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tokuoka
authors.affiliation: a Faculty of Applied Bioscience, Department of Fermentation Science and Technology , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Kikuchi
authors.affiliation: b Noda Institute for Scientific Research , Noda , Japan.
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Shinohara
authors.affiliation: a Faculty of Applied Bioscience, Department of Fermentation Science and Technology , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Koyama
authors.affiliation: a Faculty of Applied Bioscience, Department of Fermentation Science and Technology , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Iio
authors.affiliation: c Graduate School of Pharmaceutical Sciences , Hokkaido University , Sapporo , Japan.|d Showa Pharmaceutical University , Tokyo , Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kubota
authors.affiliation: c Graduate School of Pharmaceutical Sciences , Hokkaido University , Sapporo , Japan.
authors.forename: Jun'ichi
authors.initials: J
authors.lastname: Kobayashi
authors.affiliation: b Noda Institute for Scientific Research , Noda , Japan.
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Koyama
authors.affiliation: e Research Institute of KANSEI science , Yokohama , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Totsuka
authors.affiliation: a Faculty of Applied Bioscience, Department of Fermentation Science and Technology , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shindo
authors.affiliation: a Faculty of Applied Bioscience, Department of Fermentation Science and Technology , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sato
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D007211:Indoles; C000609952:speradine A; C000543:cyclopiazonic acid
keywords: Aspergillus oryzae; cyclopiazonic acid; secondary metabolism; speradine A