title: Optically-tracked handheld fluorescence imaging platform for monitoring skin response in the management of soft tissue sarcoma.
abstract: Standard clinical management of extremity soft tissue sarcomas includes surgery with radiation therapy. Wound complications (WCs) arising from treatment may occur due to bacterial infection and tissue breakdown. The ability to detect changes in these parameters during treatment may lead to earlier interventions that mitigate WCs. We describe the use of a new system composed of an autofluorescence imaging device and an optical three-dimensional tracking system to detect and coregister the presence of bacteria with radiation doses. The imaging device visualized erythema using white light and detected bacterial autofluorescence using 405-nm excitation light. Its position was tracked relative to the patient using IR reflective spheres and registration to the computed tomography coordinates. Image coregistration software was developed to spatially overlay radiation treatment plans and dose distributions on the white light and autofluorescence images of the surgical site. We describe the technology, its use in the operating room, and standard operating procedures, as well as demonstrate technical feasibility and safety intraoperatively. This new clinical tool may help identify patients at greater risk of developing WCs and investigate correlations between radiation dose, skin response, and changes in bacterial load as biomarkers associated with WCs.
authors.affiliation: University Health Network, Princess Margaret Cancer Centre, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
authors.forename: Emilie
authors.initials: E
authors.lastname: Chamma
authors.affiliation: Princess Margaret Cancer Centre, Radiation Medicine Program, 610 University Avenue, Toronto M5G 2M9, Canada.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Qiu
authors.affiliation: University Health Network, Princess Margaret Cancer Centre, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
authors.forename: Liis
authors.initials: L
authors.lastname: Lindvere-Teene
authors.affiliation: University Health Network, Princess Margaret Cancer Centre, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
authors.forename: Kristina M
authors.initials: KM
authors.lastname: Blackmore
authors.affiliation: University Health Network, Princess Margaret Cancer Centre, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
authors.forename: Safa
authors.initials: S
authors.lastname: Majeed
authors.affiliation: University Health Network, Princess Margaret Cancer Centre, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, CanadabPrincess Margaret Cancer Centre, Radiation Medicine Program, 610 University Avenue, Toronto M5G.
authors.forename: Robert
authors.initials: R
authors.lastname: Weersink
authors.affiliation: Princess Margaret Cancer Centre, Radiation Medicine Program, 610 University Avenue, Toronto M5G 2M9, CanadadPrincess Margaret Cancer Centre, Department of Radiation Oncology, 610 University Avenue, Toronto M5G 2M9, Canada.
authors.forename: Colleen I
authors.initials: CI
authors.lastname: Dickie
authors.affiliation: Division of Orthopedic Surgery, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Griffin
authors.affiliation: Division of Orthopedic Surgery, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, CanadafLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada.
authors.forename: Jay S
authors.initials: JS
authors.lastname: Wunder
authors.affiliation: Division of Orthopedic Surgery, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, CanadafLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Ferguson
authors.affiliation: University Health Network, Princess Margaret Cancer Centre, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, CanadacUniversity Health Network, Techna Institute, 124-100 College Street, Toronto, Ontario M5G 1P5, C.
authors.forename: Ralph S
authors.initials: RS
authors.lastname: DaCosta
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D004867:Equipment Design; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D012509:Sarcoma / Q000532:radiotherapy*; D012983:Soft Tissue Neoplasms / Q000532:radiotherapy*; D013530:Surgical Wound Infection / Q000175:diagnosis* / Q000382:microbiology