title: Molecular Cloning and Co-Expression of Phytoene Synthase Gene from Kocuria gwangalliensis in Escherichia coli.
abstract: A phytoene synthase gene, crtB, was isolated from Kocuria gwangalliensis. The crtB with 1,092 bp full-length has a coding sequence of 948 bp and encodes a 316-amino-acids protein. The deduced amino acid sequence showed a 70.9% identity with a putative phytoene synthase from K. rhizophila. An expression plasmid, pCcrtB, containing the crtB gene was constructed, and E. coli cells containing this plasmid produced the recombinant protein of approximately 34 kDa , corresponding to the molecular mass of phytoene synthase. Biosynthesis of lycopene was confirmed when the plasmid pCcrtB was co-transformed into E. coli containing pRScrtEI carrying the crtE and crtI genes encoding lycopene biosynthetic pathway enzymes. The results obtained from this study will provide a base of knowledge about the phytoene synthase of K. gwangalliensis and can be applied to the production of carotenoids in a non-carotenoidproducing host.
authors.affiliation: Institute of Fisheries Science, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Yong Bae
authors.initials: YB
authors.lastname: Seo
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Seong-Seok
authors.initials: SS
authors.lastname: Choi
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Jong Kyu
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Nan-Hee
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Department of Marine Biomaterials and Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: Department of Marine Biomaterials and Aquaculture, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Jong-Myoung
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Marine and Fisheries Resources, Mokpo National University, 1666 Youngsanro, Muan, Jeonnam 534-729, Republic of Korea.
authors.forename: Tae Hyug
authors.initials: TH
authors.lastname: Jeong
authors.affiliation: Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Republic of Korea.
authors.forename: Soo-Wan
authors.initials: SW
authors.lastname: Nam
authors.affiliation: Department of Marine and Fisheries Resources, Mokpo National University, 1666 Youngsanro, Muan, Jeonnam 534-729, Republic of Korea.
authors.forename: Han Kyu
authors.initials: HK
authors.lastname: Lim
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Gun-Do
authors.initials: GD
authors.lastname: Kim
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000077276:Lycopene; D008835:Micrococcaceae / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D011994:Recombinant Proteins; D002338:Carotenoids; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D000077276:Lycopene
keywords: Kocuria gwangalliensis; carotenoid; lycopene; phytoene; phytoene synthase