title: A candidate packaging signal of human rotavirus differentiating Wa-like and DS-1-like genomic constellations.
abstract: Rotavirus A (RVA) possesses a genome of 11 segmented RNAs. In human RVA, two major genomic constellations are represented by prototype strains Wa and DS-1. Here packaging signals differentiating Wa-like and DS-1-like genomic constellations were searched for by analyzing genomic sequences of Wa-like and DS-1-like strains. One pair of 11 nucleotide sites in the coding regions of viral structural protein (VP) 2 and VP6 was found to be complementary specifically among Wa-like strains. These sites tended to be free from base-pairing in secondary structures of genomic segments, suggesting that they may serve as a packaging signal in Wa-like strains.
authors.affiliation: Graduate School of Natural Sciences, Nagoya City University, 1 Yamanohata, Mizuho-cho, Mizuho-ku, Nagoya-shi, Aichi-ken 467-8501, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D016679:Genome, Viral; D006801:Humans; D059372:Nucleotide Motifs; D012401:Rotavirus / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly
keywords: complementarity; human rotavirus; packaging signal; secondary structure