title: A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease.
abstract: The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.
authors.affiliation: Department of Chemistry , National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore).|Key Laboratory of Flexible Electronics & Institute of Advanced Materials, National Jiangsu Synergistic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, 211816 (P.R. China).
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: National Neuroscience Institute, Singapore 308433 (Singapore).
authors.forename: Cheng-Wu
authors.initials: CW
authors.lastname: Zhang
authors.affiliation: Department of Chemistry , National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore).
authors.forename: Jingyan
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Chemistry , National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore).
authors.forename: Linghui
authors.initials: L
authors.lastname: Qian
authors.affiliation: National Neuroscience Institute, Singapore 308433 (Singapore).
authors.forename: Bing-Han
authors.initials: BH
authors.lastname: Chai
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014 (P.R. China).
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), and Department of Biological Chemistry, University of Science & Technology (Republic of Korea).
authors.forename: Jun-Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: National Neuroscience Institute, Singapore 308433 (Singapore).
authors.forename: Kah-Leong
authors.initials: KL
authors.lastname: Lim
authors.affiliation: Department of Chemistry , National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore). chmyaosq@nus.edu.sg.
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D015335:Molecular Probes; D008995:Monoamine Oxidase / Q000378:metabolism*; D010300:Parkinson Disease / Q000201:enzymology*
chemical_list: D015335:Molecular Probes; D008995:Monoamine Oxidase
keywords: Parkinson’s disease; activity-based probes; imaging; monoamine oxidases; proteomics