title: Determination of five sesquiterpenoids in Xingnaojing injection by quantitative analysis of multiple components with a single marker.
abstract: A quantitative analysis of multiple components with a single-marker method was established for the simultaneous determination of five sesqutiterpenoids in Xingnaojing injection. This method was established with Xingnaojing injection determined by high-performance liquid chromatography coupled with diode array detection. The durability and system suitability of the established method were evaluated, and the reliable relative correction factors were obtained with curdione selected as an internal reference. The contents of the five components in all Xingnaojing injections were determined by external standard method and the contents of curcumenone, curcumenol, curzerenone, and germacrone were also calculated with the obtained relative correction factors. Then, relative error was investigated to estimate the difference of the two methods. As a result, the established new method possesses good adaptability, and there is no significant difference between the two methods, except for the content of curzerenone in eight samples. To put the established method into practice, the limits of quantitation of the established method of the five components were proposed and defined. Thus, the developed methodology can also be utilized to the quality evaluation of Xingnaojing injection, in spite of the difference found in the content of curzerenone between the external standard method and the newly established method.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.|National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing, China.|Chengde Medical University, Chengde, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Pan
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.|National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.|National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Feng
authors.affiliation: Hunan University of Chinese Medicine, Changsha, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Lin
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.|National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Wuxi Jiminkexin Shanhe Pharmaceutical Co., Ltd, Wuxi, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Wuxi Jiminkexin Shanhe Pharmaceutical Co., Ltd, Wuxi, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Gan
authors.affiliation: Wuxi Jiminkexin Shanhe Pharmaceutical Co., Ltd, Wuxi, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Fan
authors.affiliation: Wuxi Jiminkexin Shanhe Pharmaceutical Co., Ltd, Wuxi, China.
authors.forename: Jigao
authors.initials: J
authors.lastname: Zou
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.|National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Chengde Medical University, Chengde, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Pan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000592:standards; D006801:Humans; D007267:Injections; D010936:Plant Extracts / Q000032:analysis; D011786:Quality Control; D012015:Reference Standards; D012717:Sesquiterpenes / Q000032:analysis* / Q000592:standards; D045788:Sesquiterpenes, Germacrane / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C552245:curcumenol; C582256:curcumenone; C504126:curdione; C000594497:xingnaojing; C048393:germacrone
keywords: Content determination; Herbal medicine; High-performance liquid chromatography; Sesquiterpenoids; Xingnaojing injection