title: Proteomic Analysis Reveals Distinct Metabolic Differences Between Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF) Grown Macrophages Derived from Murine Bone Marrow Cells.
abstract: Macrophages are crucial in controlling infectious agents and tissue homeostasis. Macrophages require a wide range of functional capabilities in order to fulfill distinct roles in our body, one being rapid and robust immune responses. To gain insight into macrophage plasticity and the key regulatory protein networks governing their specific functions, we performed quantitative analyses of the proteome and phosphoproteome of murine primary GM-CSF and M-CSF grown bone marrow derived macrophages (GM-BMMs and M-BMMs, respectively) using the latest isobaric tag based tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Strikingly, metabolic processes emerged as a major difference between these macrophages. Specifically, GM-BMMs show significant enrichment of proteins involving glycolysis, the mevalonate pathway, and nitrogen compound biosynthesis. This evidence of enhanced glycolytic capability in GM-BMMs is particularly significant regarding their pro-inflammatory responses, because increased production of cytokines upon LPS stimulation in GM-BMMs depends on their acute glycolytic capacity. In contrast, M-BMMs up-regulate proteins involved in endocytosis, which correlates with a tendency toward homeostatic functions such as scavenging cellular debris. Together, our data describes a proteomic network that underlies the pro-inflammatory actions of GM-BMMs as well as the homeostatic functions of M-BMMs.
authors.affiliation: From the ‡Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-799, South Korea;
authors.forename: Yi Rang
authors.initials: YR
authors.lastname: Na
authors.affiliation: §Department of Applied Chemistry, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, South Korea;
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Hong
authors.affiliation: §Department of Applied Chemistry, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, South Korea;
authors.forename: Min Yong
authors.initials: MY
authors.lastname: Lee
authors.affiliation: §Department of Applied Chemistry, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, South Korea;
authors.forename: Jae Hun
authors.initials: JH
authors.lastname: Jung
authors.affiliation: From the ‡Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-799, South Korea;
authors.forename: Daun
authors.initials: D
authors.lastname: Jung
authors.affiliation: From the ‡Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-799, South Korea;
authors.forename: Young Won
authors.initials: YW
authors.lastname: Kim
authors.affiliation: From the ‡Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-799, South Korea;
authors.forename: Dain
authors.initials: D
authors.lastname: Son
authors.affiliation: ¶Department of Biomedical Science, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-799, South Korea.
authors.forename: Murim
authors.initials: M
authors.lastname: Choi
authors.affiliation: §Department of Applied Chemistry, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, South Korea; kimkp@khu.ac.kr lamseok@snu.ac.kr.
authors.forename: Kwang Pyo
authors.identifier: http://orcid.org/0000-0003-0095-3787
authors.initials: KP
authors.lastname: Kim
authors.affiliation: From the ‡Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-799, South Korea;
authors.forename: Seung Hyeok
authors.initials: SH
authors.lastname: Seok
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D006019:Glycolysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D008863:Microspheres; D010587:Phagocytosis; D020543:Proteome / Q000378:metabolism; D040901:Proteomics
chemical_list: D016207:Cytokines; D020543:Proteome; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor