title: Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
abstract: Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane. A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h. The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
authors.affiliation: Students Research Committee, Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, Iran. Electronic address: r.mohammadi@sbmu.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, Iran. Electronic address: Mohamdif@ut.ac.ir.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Mohammadifar
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, Iran. Electronic address: mortazvn@sbmu.ac.ir.
authors.forename: Amir Mohammad
authors.initials: AM
authors.lastname: Mortazavian
authors.affiliation: Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, College of Agriculture & Natural Resources, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran. Electronic address: mldrouhi@ut.ac.ir.
authors.forename: Milad
authors.initials: M
authors.lastname: Rouhi
authors.affiliation: Faculty of Chemistry, University of Tehran, Tehran, Iran. Electronic address: jahan.ghasemi@gmail.com.
authors.forename: Jahan B
authors.initials: JB
authors.lastname: Ghasemi
authors.affiliation: Students Research Committee, Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, Iran.
authors.forename: Zohre
authors.initials: Z
authors.lastname: Delshadian
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D003094:Collagen / Q000737:chemistry*; D004528:Egg Shell / Q000737:chemistry*; D010434:Pepsin A / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D003094:Collagen; D010434:Pepsin A
keywords: Eggshell membrane; Pepsin-soluble collagen; Response surface methodology