title: Characterization of vitamin-cisplatin-loaded chitosan nano-particles for chemoprevention and cancer fatigue.
abstract: BACKGROUND
  Vitamins have been shown to reduce chemotherapy-related fatigue (CRF) by conserving energy loss both during and after cancer treatment. However, it remains unknown whether this reduction of fatigue interferes with the cancer drugs or alters the effectiveness of these agents.
  
  
  OBJECTIVE
  The objective was to synthesize vitamin-cisplatin-loaded chitosan nano-particles for chemoprevention and cancer fatigue.
  
  
  METHODS
  Multi-vitamin (C, D3, and B12)-cisplatin composite nano-formulation called NanoCisVital (NCV) to overcome CRF. The interactions between vitamins and NCV were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, and a particle size analyser. The chemo-preventive activity was performed by in vitro bio assays.
  
  
  RESULTS
  SEM analysis showed spherical shape and the size is < 225 nm. NCV inhibited the proliferating yeast cells as well as denaturation of bovine serum albumin, and it also reduced the sprouting of new blood vessels in dose-dependent manner.
  
  
  CONCLUSIONS
  Collectively, these results demonstrate that the NCV particles can be used to reduce CRF without much affecting the anti-cancer properties of cisplatin.
authors.affiliation: a Biomedical Research Laboratory, Department of Biotechnology, K L E F University (Koneru Lakshmaiah Education Foundation) Vaddeswaram , Guntur , Andhra Pradesh , India .
authors.forename: Rajath
authors.initials: R
authors.lastname: Othayoth
authors.forename: Pardhasaradhi
authors.initials: P
authors.lastname: Mathi
authors.forename: Khaggeswar
authors.initials: K
authors.lastname: Bheemanapally
authors.forename: Lavanya
authors.initials: L
authors.lastname: Kakarla
authors.forename: Mahendran
authors.initials: M
authors.lastname: Botlagunta
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D016588:Anticarcinogenic Agents / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry; D001681:Biological Assay; D002417:Cattle; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D002945:Cisplatin / Q000008:administration & dosage*; D005221:Fatigue / Q000139:chemically induced / Q000150:complications; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000150:complications / Q000188:drug therapy* / Q000517:prevention & control*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014815:Vitamins / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D016588:Anticarcinogenic Agents; D000970:Antineoplastic Agents; D014815:Vitamins; D012710:Serum Albumin, Bovine; D048271:Chitosan; D002945:Cisplatin
keywords: Anti-proliferative; FT-IR; SEM; cancer-related fatigue; chemoprevention; encapsulation efficiency; haemolytic activity; nano-formulation