title: Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes.
abstract: Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.
authors.affiliation: Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
authors.forename: Viviane
authors.initials: V
authors.lastname: Chenal-Francisque
authors.affiliation: Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France Paris Diderot University, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.
authors.forename: Mylène M
authors.initials: MM
authors.lastname: Maury
authors.affiliation: Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Lavina
authors.affiliation: Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Touchon
authors.affiliation: Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Leclercq
authors.affiliation: Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France INSERM Unit 1117, Paris, France Paris Descartes University, Sorbonne Paris Cité, Institut Imagine, Necker-Enfants Malades University Hospital, Division of Infectious Diseases and Tropical Medicine, APHP, Paris, France sylvain.brisse@pasteur.fr marc.lecuit@pasteur.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Lecuit
authors.affiliation: Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France sylvain.brisse@pasteur.fr marc.lecuit@pasteur.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
mesh_terms: D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008088:Listeriosis / Q000175:diagnosis / Q000382:microbiology; D058889:Molecular Typing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors