title: Determination of clenbuterol in pork and potable water samples by molecularly imprinted polymer through the use of covalent imprinting method.
abstract: A novel molecularly imprinted polymer (MIP) for efficient separation and concentration of clenbuterol (CLB) was synthesized by covalent imprinting approach using CLB derivative as functional monomer. The MIPs synthesized were characterized by scanning electron microscope, nitrogen adsorption analysis, Fourier transform infrared spectrometer, and thermo-gravimetric analysis. The binding experimental results showed that the MIPs synthesized had fast adsorption kinetic (20 min at 25 mg L(-1)), high adsorption capacity and specific recognition ability for the analyte. In addition, the MIPs synthesized were successfully used as solid-phase sorbent for CLB sample preparation to be analyzed by high performance liquid chromatography with ultraviolet detector. Under optimized experimental conditions, the linear range of the calibration curve was 5-80 μg L(-1) (R(2) = 0.9938). The proposed method was also applied to the analysis of CLB in pork and potable water samples.
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Jianxing
authors.initials: J
authors.lastname: Lan
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Liqiao
authors.initials: L
authors.lastname: Wei
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Food Science and Engineering, Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China. Electronic address: tianlanyijiu81@163.com.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002976:Clenbuterol / Q000032:analysis*; D060766:Drinking Water / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D000069466:Red Meat / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D013552:Swine; D013818:Thermogravimetry
chemical_list: D060766:Drinking Water; D011108:Polymers; D002976:Clenbuterol
keywords: Clenbuterol; Covalent molecularly imprinted polymers; Pork; Solid-phase extraction; Water