title: Chemical generation and modification of peptides containing multiple dehydroalanines.
abstract: Chemical formation of dehydroalanine has been widely used for the post-translational modification of proteins and peptides, however methods to incorporate multiple dehydroalanine residues into a single peptide have not been defined. We report the use of methyl 2,5-dibromovalerate which can be used to cleanly carry out this transformation.
authors.affiliation: School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK. m.e.webb@leeds.ac.uk.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Morrison
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Foley
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Warriner
authors.forename: Michael E
authors.initials: ME
authors.lastname: Webb
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D006801:Humans; D007610:Kallikreins / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism; D014631:Valerates / Q000737:chemistry
chemical_list: D010455:Peptides; D014631:Valerates; C015102:dehydroalanine; D007610:Kallikreins; D000409:Alanine