title: Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood.
abstract: We report a transient absorption (TA) imaging method for fast visualization and quantitative layer analysis of graphene and GO. Forward and backward imaging of graphene on various substrates under ambient condition was imaged with a speed of 2 μs per pixel. The TA intensity linearly increased with the layer number of graphene. Real-time TA imaging of GO in vitro with capability of quantitative analysis of intracellular concentration and ex vivo in circulating blood were demonstrated. These results suggest that TA microscopy is a valid tool for the study of graphene based materials.
authors.affiliation: Purdue University Interdisciplinary Life Sciences Program (PULSe), Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Weixia
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Physics and Astronomy, Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Ting-Fung
authors.initials: TF
authors.lastname: Chung
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Mikhail N
authors.initials: MN
authors.lastname: Slipchenko
authors.affiliation: 1] Department of Physics and Astronomy, Purdue University, West Lafayette, Indiana, 47907, USA [2] Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Yong P
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
authors.affiliation: 1] Department of Chemistry, Purdue University, West Lafayette, Indiana, 47907, USA [2] Department of Physics and Astronomy, Purdue University, West Lafayette, Indiana, 47907, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
mesh_terms: D000818:Animals; D003199:Computer Systems; D006108:Graphite / Q000032:analysis* / Q000097:blood*; D007089:Image Enhancement / Q000379:methods; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D010087:Oxides / Q000032:analysis* / Q000097:blood*; D051381:Rats; D020318:Rats, Long-Evans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010087:Oxides; D006108:Graphite