title: Noninvasive theranostic imaging of HSV-TK/GCV suicide gene therapy in liver cancer by folate-targeted quantum dot-based liposomes.
abstract: Theranostics is emerging as a popular strategy for cancer therapy; thanks to the development of nanotechnology. In this work, we have combined an HSV-TK/GCV suicide gene system and near-infrared quantum dots, as the former is quite effective in liver cancer treatment and the latter facilitates tumor imaging. A folate-modified theranostic liposome (FL/QD-TK) was developed, which is composed of an HSV-TK suicide gene covalently coupling with near-infrared fluorescent CdSeTe/ZnS core/shell quantum dots. The liver cancer-targeting and biosafety of FL/QD-TK were studied in vitro and in vivo. FL/QD-TK exhibited highly specific tumor imaging and strong inhibition of the folate receptor-overexpressed Bel-7402 mouse xenografts without systematic toxicity. This study may shed light on gene delivery and targeted cancer therapy.
authors.affiliation: Department of Pharmacology, Nanomedicine Engineering Laboratory of Jilin Province, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. chenl@jlu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Shao
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry / Q000187:drug effects*; D003952:Diagnostic Imaging; D005492:Folic Acid / Q000737:chemistry*; D015774:Ganciclovir / Q000737:chemistry / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D041722:Genes, Transgenic, Suicide / Q000187:drug effects* / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D056945:Hep G2 Cells / Q000737:chemistry* / Q000187:drug effects*; D006801:Humans; D008081:Liposomes; D008113:Liver Neoplasms / Q000737:chemistry* / Q000188:drug therapy*; D051379:Mice; D036103:Nanotechnology; D045663:Quantum Dots / Q000737:chemistry*; D018139:Simplexvirus / Q000187:drug effects* / Q000235:genetics; D000068936:Theranostic Nanomedicine; D013937:Thymidine Kinase / Q000737:chemistry / Q000235:genetics*
chemical_list: D000998:Antiviral Agents; D008081:Liposomes; D005492:Folic Acid; D013937:Thymidine Kinase; D015774:Ganciclovir