title: Lipidomic differentiation between human kidney tumors and surrounding normal tissues using HILIC-HPLC/ESI-MS and multivariate data analysis.
abstract: The characterization of differences among polar lipid classes in tumors and surrounding normal tissues of 20 kidney cancer patients is performed by hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization mass spectrometry (ESI-MS). The detailed analysis of identified lipid classes using relative abundances of characteristic ions in negative- and positive-ion modes is used for the determination of more than 120 individual lipid species containing attached fatty acyls of different chain length and double bond number. Lipid species are described using relative abundances, providing a better visualization of lipidomic differences between tumor and normal tissues. The multivariate data analysis methods using unsupervised principal component analysis (PCA) and supervised orthogonal partial least square (OPLS) are used for the characterization of statistically significant differences in identified lipid species. Ten most significant up- and down-regulated lipids in OPLS score plots are also displayed by box plots. A notable increase of relative abundances of lipids containing four and more double bonds is detected in tumor compared to normal tissues.
authors.affiliation: University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Cífková
authors.affiliation: University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Studentská 573, 532 10 Pardubice, Czech Republic. Electronic address: Michal.Holcapek@upce.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Holčapek
authors.affiliation: University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Lísa
authors.affiliation: Palacký University, Medical School and Teaching Hospital, Department of Oncology, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Vrána
authors.affiliation: Palacký University, Medical School and Teaching Hospital, Department of Oncology, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Melichar
authors.affiliation: Palacký University, Faculty of Medicine and Dentistry, Department of Urology, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Študent
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019295:Computational Biology; D020404:Glycerophospholipids; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000032:analysis*; D015999:Multivariate Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D020404:Glycerophospholipids; D008055:Lipids
keywords: Glycerophospholipids; HILIC-HPLC/ESI–MS; Kidney cancer; Lipidomics; Multivariate data analysis