title: Neuregulin protects human umbilical vein endothelial cell via activating CD98hc through MAPK pathway.
abstract: BACKGROUND
  To study the effects of neuregulin on human umbilical vein endothelial cells (HUVEC) protection.
  
  
  METHODS
  The HUEVC were cultured and divided into three different groups according to their culture conditions. In negative control group (group A), HUVEC were cultured in dulbecco modified eagle medium (DMEM) supplemented with 25 mmol/L glucose and neuregulin (10 ng/ml, 100 ng/ml, and 1000 ng/ml, respectively) for 48 hr. In experimental group (group B), HUVEC were cultured in DMEM with 75 mmol/L glucose and neuregulin (10 ng/ml, 100 ng/ml, and 1000 ng/ml, respectively) for 48 hr. In positive control group (group C), HUVEC were cultured in DMEM supplemented with 75 mmol/L mannitol, 25 mmol/L glucose, and neuregulin (10 ng/ml, 100 ng/ml, and 1000 ng/ml, respectively) for 48 hr. This study aimed to observe the cell morphology in different HUEVC groups and analyze apoptosis in each group via characterizing and detecting the protein expressions of CD98hc, p38, and JNK.
  
  
  RESULTS
  Significant differences in cell morphology were observed in the experimental group when compared with the control groups. In the experimental group, the degree of apoptosis was negatively related to the neuregulin concentration; CD98hc protein concentration was positively related to neuregulin concentration; JNK protein concentration was positively related to neuregulin concentration. However, there was no significant relationship between p38 protein expression and the concentration of glucose in the medium.
  
  
  CONCLUSIONS
  Neuregulin could inhibit the apoptosis of HUEVC through the activation of MAPK signaling pathway via activating CD98hc expression.
authors.affiliation: Department of Cardiology, Second Xiangya Hospital, Central South University Changsha, 410011, Hunan, China ; Department of Cardiology, Northern Jiangsu People's Hospital, Medical College of Yangzhou University Yangzhou, 225001, Jiangsu, China.
authors.forename: Shenghu
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Cardiology, Second Xiangya Hospital, Central South University Changsha, 410011, Hunan, China ; Department of Cardiology, Northern Jiangsu People's Hospital, Medical College of Yangzhou University Yangzhou, 225001, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Hospital of Jiangsu Army Police Yangzhou, 225001, Jiangsu, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Cardiology, Northern Jiangsu People's Hospital, Medical College of Yangzhou University Yangzhou, 225001, Jiangsu, China.
authors.forename: Daxin
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Northern Jiangsu People's Hospital, Medical College of Yangzhou University Yangzhou, 225001, Jiangsu, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Second Xiangya Hospital, Central South University Changsha, 410011, Hunan, China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Zhou
keywords: Human umbilical vein endothelial cells; MAPK signaling pathway; apoptosis; high glucose; neuregulin