title: Quantitative analysis of the Escherichia coli proteome.
abstract: Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.
authors.affiliation: Biochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
authors.forename: Jacek R
authors.initials: JR
authors.lastname: Wiśniewski
authors.affiliation: Department of Animal Molecular Physiology, Wroclaw University, Wroclaw, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Rakus
keywords: Absolute protein quantification; Escherichia coli proteome; Filter-aided sample preparation, FASP; Protein copy number; Total protein approach