title: Determinants of Human CD134 Essential for Entry of Human Herpesvirus 6B.
abstract: We identified two key amino acid residues within human CD134 (hCD134) that are required for its interaction with human herpesvirus 6B (HHV-6B) and for HHV-6B entry into cells. One of the residues (K79) allows access of the HHV-6B ligand to hCD134. Murine CD134 (mCD134) functioned as an HHV-6B receptor when these two amino acid residues were replaced with homologous human residues. This study identifies both the HHV-6B receptor-ligand interaction and the species-specific determinants of hCD134 essential for HHV-6B entry.
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan Department of Immunology, Nanjing Medical University, Nanjing, China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Tang
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan ymori@med.kobe-u.ac.jp.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D018360:Crystallography, X-Ray; D015654:Herpesvirus 6, Human / Q000472:pathogenicity / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D006801:Humans; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D053262:Receptors, OX40 / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D053586:Virus Internalization
chemical_list: D008024:Ligands; D053262:Receptors, OX40; D011991:Receptors, Virus; C505716:TNFRSF4 protein, human; C505717:Tnfrsf4 protein, mouse