title: Gene expression profile of ABC transporters and cytotoxic effect of ibuprofen and acetaminophen in an epithelial ovarian cancer cell line in vitro.
abstract: OBJECTIVE
  To determine the basic expression of ABC transporters in an epithelial ovarian cancer cell line, and to investigate whether low concentrations of acetaminophen and ibuprofen inhibited the growth of this cell line in vitro.
  
  
  METHODS
  TOV-21 G cells were exposed to different concentrations of acetaminophen (1.5 to 15 μg/mL) and ibuprofen (2.0 to 20 μg/mL) for 24 to 48 hours. The cellular growth was assessed using a cell viability assay. Cellular morphology was determined by fluorescence microscopy. The gene expression profile of ABC transporters was determined by assessing a panel including 42 genes of the ABC transporter superfamily.
  
  
  RESULTS
  We observed a significant decrease in TOV-21 G cell growth after exposure to 15 μg/mL of acetaminophen for 24 (p=0.02) and 48 hours (p=0.01), or to 20 μg/mL of ibuprofen for 48 hours (p=0.04). Assessing the morphology of TOV-21 G cells did not reveal evidence of extensive apoptosis. TOV-21 G cells had a reduced expression of the genes ABCA1, ABCC3, ABCC4, ABCD3, ABCD4 and ABCE1 within the ABC transporter superfamily.
  
  
  CONCLUSIONS
  This study provides in vitro evidence of inhibitory effects of growth in therapeutic concentrations of acetaminophen and ibuprofen on TOV-21 G cells. Additionally, TOV-21 G cells presented a reduced expression of the ABCA1, ABCC3, ABCC4, ABCD3, ABCD4 and ABCE1 transporters.
authors.affiliation: Hospital das Clínicas de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Renilton Aires
authors.initials: RA
authors.lastname: Lima
authors.affiliation: Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Eduardo Batista
authors.initials: EB
authors.lastname: Cândido
authors.affiliation: Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
authors.forename: Flávia Perrim
authors.initials: FP
authors.lastname: de Melo
authors.affiliation: Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
authors.forename: Josiane Barbosa
authors.initials: JB
authors.lastname: Piedade
authors.affiliation: Department of Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Paula Vieira Teixeira
authors.initials: PV
authors.lastname: Vidigal
authors.affiliation: Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
authors.forename: Luciana Maria
authors.initials: LM
authors.lastname: Silva
authors.affiliation: Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Agnaldo Lopes
authors.initials: AL
authors.lastname: da Silva Filho
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000082:Acetaminophen / Q000494:pharmacology*; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology*; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D059467:Transcriptome / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D018528:ATP-Binding Cassette Transporters; D000082:Acetaminophen; D007052:Ibuprofen