title: Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications.
abstract: Mobilized peripheral blood (MPB) bone marrow cells possess the potential to differentiate into a variety of mesenchymal tissue types and offer a source of easy access for obtaining stem cells for the development of experimental models with applications in tissue engineering. In the present work, we aimed to isolate by magnetic activated cell sorting CD90+ cells from MPB by means of the administration of Granulocyte-Colony Stimulating Factor and to evaluate cell proliferation capacity, after thawing of the in vitro culture of this population of mesenchymal stem cells (MSCs) in sheep. We obtained a median of 8.2 ± 0.6 million of CD90+ cells from the 20-mL MPB sample. After thawing, at day 15 under in vitro culture, the mean CD90+ cells determined by flow cytometry was 92.92 ± 1.29 % and cell duplication time determined by crystal violet staining was 47.59 h. This study describes for the first time the isolation, characterization, and post-in vitro culture thawing of CD90+ MSCs from mobilized peripheral blood in sheep. This population can be considered as a source of MSCs for experimental models in tissue engineering research.
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Landa-Solís
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Julio
authors.initials: J
authors.lastname: Granados-Montiel
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Anell
authors.initials: A
authors.lastname: Olivos-Meza
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Carmina
authors.initials: C
authors.lastname: Ortega-Sánchez
authors.affiliation: Department of Fetal Medicine and Surgery, Women and Children's Specialty Hospital of Queretaro, Queretaro, Mexico.
authors.forename: Mónica
authors.initials: M
authors.lastname: Cruz-Lemini
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Hernández-Flores
authors.affiliation: National Institute of Pediatrics, Mexico City, Mexico.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Chang-González
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Ricardo Gómez
authors.initials: RG
authors.lastname: García
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Brenda
authors.initials: B
authors.lastname: Olivos-Díaz
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: María Cristina
authors.initials: MC
authors.lastname: Velasquillo-Martínez
authors.affiliation: Research Direction, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pineda
authors.affiliation: Department of Tissue Engineering, Cell Therapy and Regenerative Medicine, National Rehabilitation Institute, Av México-Xochimilco 289, Col. Arenal de Guadalupe, CP 14389, Mexico City, Mexico. tissuegroup.inr@gmail.com.
authors.forename: Clemente
authors.initials: C
authors.lastname: Ibarra
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D015925:Cryopreservation / Q000379:methods*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D000072916:Peripheral Blood Stem Cells / Q000166:cytology*; D012756:Sheep; D018800:Thy-1 Antigens / Q000378:metabolism*
chemical_list: D018800:Thy-1 Antigens
keywords: Cryopreserved CD90+ cells; Mesenchymal stem cells; Mobilized peripheral blood