title: Mesoporous materials modified by aptamers and hydrophobic groups assist ultra-sensitive insulin detection in serum.
abstract: A novel mesoporous material modified with both insulin-binding-aptamers and hydrophobic methyl groups is synthesized. With rationally designed pore structures and surface chemistry, this material is applied in sample pre-treatment for ELISA, and enables the quantification (0.25-5 pg ml(-1)) of insulin in serum, 30-fold enhancement of the limit-of-detection compared to the commercial ELISA kit.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. c.yu@uq.edu.au.
authors.forename: Chang
authors.initials: C
authors.lastname: Lei
authors.forename: Chun
authors.initials: C
authors.lastname: Xu
authors.forename: Owen
authors.initials: O
authors.lastname: Noonan
authors.forename: Anand Kumar
authors.initials: AK
authors.lastname: Meka
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.forename: Amanda
authors.initials: A
authors.lastname: Nouwens
authors.forename: Chengzhong
authors.initials: C
authors.lastname: Yu
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001774:Blood Chemical Analysis / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D007328:Insulin / Q000097:blood*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D016062:Porosity
chemical_list: D052157:Aptamers, Nucleotide; D007328:Insulin; D009841:Oligonucleotides; D006735:Horseradish Peroxidase