title: Typing of Staphylococcus aureus isolated from bovine mastitis cases in Australia and India.
abstract: OBJECTIVE
  To determine the prevalence of the different capsular polysaccharide (CP) and major surface-associated non-CP antigen 336 (SP-336) types among Staphylococcus aureus isolated from bovine mastitis cases in Australia and India.
  
  
  METHODS
  A total of 414 strains (154 from Australia, 260 from India) isolated from clinical bovine mastitis were included in the study. Mouse antisera raised against CP types (CP1, CP2, CP5, and CP8) or SP-336 were used in slide agglutination tests and compared with detection of cap1, cap5 and cap8 gene fragments by PCR.
  
  
  RESULTS
  Serological studies revealed the presence of CP2, CP5, CP8 and SP-336 in 9.1%, 23.4%, 31.8%, and 5.8% of the Australian versus 0.8%, 46.9%, 13.1% and 0% of the Indian isolates, respectively. By PCR, CP1, CP5 and CP8 accounted for 0%, 26.6% and 32.4% of the Australian versus 3.9%, 85% and 8.1% of the Indian isolates, respectively.
  
  
  CONCLUSIONS
  Both PCR and the serological method demonstrated that CP5 and CP8 are the predominant capsular types in Australia, whereas CP5 is the predominant capsular type in India. The study also demonstrated a strong correlation between both methods of typing for CP1, CP5, CP8 and non-typeable S. aureus strains. High-percentage prevalence of non-typeable isolates in both the countries highlights the importance of continued investigations of the identification of unique surface-associated polysaccharide antigens prevalent among S. aureus isolates for the formulation of CP- and SP-based vaccines for bovine mastitis.
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Gogoi-Tiwari
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Babra Waryah
authors.affiliation: Ella Foundation, Genome Valley, Shameerpet Mandal, Hyderabad, India.
authors.forename: R
authors.initials: R
authors.lastname: Sunagar
authors.affiliation: Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, India.
authors.forename: H B
authors.initials: HB
authors.lastname: Veeresh
authors.affiliation: Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, India.
authors.forename: V
authors.initials: V
authors.lastname: Nuthanalakshmi
authors.affiliation: Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, India.
authors.forename: P L
authors.initials: PL
authors.lastname: Preethirani
authors.affiliation: Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, India.
authors.forename: R
authors.initials: R
authors.lastname: Sharada
authors.affiliation: Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, India.
authors.forename: S
authors.initials: S
authors.lastname: Isloor
authors.affiliation: Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, India.
authors.forename: A
authors.initials: A
authors.lastname: Bhat
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia.
authors.forename: H
authors.initials: H
authors.lastname: Al-Salami
authors.affiliation: Ella Foundation, Genome Valley, Shameerpet Mandal, Hyderabad, India.
authors.forename: N R
authors.identifier: http://orcid.org/0000-0001-5260-2159
authors.initials: NR
authors.lastname: Hegde
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia. T.Mukkur@curtin.edu.au.
authors.forename: T K
authors.initials: TK
authors.lastname: Mukkur
mesh_terms: D000818:Animals; D001315:Australia; D016667:Bacterial Capsules / Q000145:classification; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques / Q000662:veterinary; D007194:India; D008414:Mastitis, Bovine / Q000382:microbiology*; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000145:classification / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification*
chemical_list: D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial
keywords: capsular and polysaccharide antigen 336; dairy cattle; mastitis; molecular typing; serology