title: Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil.
abstract: Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.
authors.forename: Jefferson José da Silva
authors.initials: JJ
authors.lastname: Santos
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.
authors.forename: Tereza
authors.initials: T
authors.lastname: Magalhães
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.
authors.forename: José Valter Joaquim
authors.initials: JV
authors.lastname: Silva Junior
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.
authors.forename: Andréa Nazaré Monteiro Rangel da
authors.initials: AN
authors.lastname: Silva
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.
authors.forename: Marli Tenório
authors.initials: MT
authors.lastname: Cordeiro
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.
authors.forename: Laura Helena Vega Gonzales
authors.initials: LH
authors.lastname: Gil
mesh_terms: D001938:Brazil; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D003716:Dengue Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral