title: Age-Related Effects of Advanced Glycation End Products (Ages) in Bone Matrix on Osteoclastic Resorption.
abstract: Advanced glycation end products (AGEs) accumulate in bone extracellular matrix as people age. Previous studies have shown controversial results regarding the role of in situ AGEs accumulation in osteoclastic resorption. To address this issue, this study cultured human osteoclast cells directly on human cadaveric bone slices from different age groups (young and elderly) to warrant its relevance to in vivo conditions. The cell culture was terminated on the 3rd, 7th, and 10th day, respectively, to assess temporal changes in the number of differentiated osteoclasts, the number and size of osteoclastic resorption pits, the amount of bone resorbed, as well as the amount of matrix AGEs released in the medium by resorption. In addition, the in situ concentration of matrix AGEs at each resorption pit was also estimated based on its AGEs autofluorescent intensity. The results indicated that (1) osteoclastic resorption activities were significantly correlated with the donor age, showing larger but shallower resorption pits on the elderly bone substrates than on the younger ones; (2) osteoclast resorption activities were not significantly dependent on the in situ AGEs concentration in bone matrix, and (3) a correlation was observed between osteoclast activities and the concentration of AGEs released by the resorption. These results suggest that osteoclasts tend to migrate away from initial anchoring sites on elderly bone substrate during resorption compared to younger bone substrates. However, such behavior is not directly related to the in situ concentration of AGEs in bone matrix at the resorption sites.
authors.affiliation: School of Biological Sciences and Medical Engineering, Beihang University, Beijing, China.|Department of Mechanical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, San Antonio, TX, 78249, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, San Antonio, TX, 78249, USA.
authors.forename: Chintan
authors.initials: C
authors.lastname: Gandhi
authors.affiliation: Department of Medicine, University of Texas Health Science Center at San Antonio (UTHSCSA), San Antonio, USA.
authors.forename: Md Mizanur
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, San Antonio, TX, 78249, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Appleford
authors.affiliation: School of Biological Sciences and Medical Engineering, Beihang University, Beijing, China.
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Sun
authors.affiliation: Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, San Antonio, TX, 78249, USA. xiaodu.wang@utsa.edu.|Department of Mechanical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, San Antonio, TX, 78249, USA. xiaodu.wang@utsa.edu.
authors.forename: Xiaodu
authors.identifier: http://orcid.org/0000-0002-5600-1278
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001862:Bone Resorption / Q000378:metabolism*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D017127:Glycation End Products, Advanced / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008297:Male; D010010:Osteoclasts / Q000378:metabolism*
chemical_list: D017127:Glycation End Products, Advanced
keywords: Advanced glycation end products (AGEs); Bone; Bone resorption; Osteoclast