title: Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers.
abstract: Sugarcane smut caused by the fungus Sporisorium scitamineum is a worldwide disease and also one of the most prevalent diseases in sugarcane production in mainland China. To study molecular variation in S. scitamineum, 23 S. scitamineum isolates from the 6 primary sugar-cane production areas in mainland, China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi Provinces), were assessed using internal transcribed spacer (ITS) methods. The results of ITS sequence analysis showed that the organisms can be defined at the genus level, including Ustilago and Sporisorium, and can also differentiate between closely related species. This method was not suitable for phylogenetic relationship analysis of different S. scitamineum isolates and could not provide support regarding their race ascription at the molecular level. The results of the present study will be useful for studies examining the molecular diversity of S. scitamineum and for establishing a genetic foundation for their pathogenicity differentiation and new race detection. In addition, our results can provide useful information for the pathogen selection principle in sugarcane smut resistance breeding and variety distribution.
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: N
authors.initials: N
authors.lastname: Huang
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Xiao
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Su
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China queyouxiong@126.com.
authors.forename: Y X
authors.initials: YX
authors.lastname: Que
mesh_terms: D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D031786:Saccharum / Q000382:microbiology; D016415:Sequence Alignment
chemical_list: D021903:DNA, Ribosomal Spacer