title: MicroRNA Profiling of B Cell Subsets from Systemic Lupus Erythematosus Patients Reveals Promising Novel Biomarkers.
abstract: MicroRNAs control the differentiation and function of B cells, which are considered key elements in the pathogenesis of systemic lupus erythematosus (SLE). However, a common micro(mi)RNA signature has not emerged since published data includes patients of variable ethnic background, type of disease, and organ involvement, as well as heterogeneous cell populations. Here, we aimed at identifying a miRNA signature of purified B cells from renal and non-renal severe SLE patients of Latin American background, a population known to express severe disease. Genome-wide miRNA expression analyses were performed on naive and memory B cells and revealed two categories of miRNA signatures. The first signature represents B cell subset-specific miRNAs deregulated in SLE: 11 and six miRNAs discriminating naive and memory B cells of SLE patients from healthy controls (HC), respectively. Whether the miRNA was up or down-regulated in memory B cells as compared with naive B cells in HC, this difference was abolished in SLE patients, and vice versa. The second signature identifies six miRNAs associated with specific pathologic features affecting renal outcome, providing a further understanding for SLE pathogenesis. Overall, the present work provided promising biomarkers in molecular diagnostics for disease severity as well as potential new targets for therapeutic intervention in SLE.
authors.affiliation: INSERM, U1183, Institute of Regenerative Medicine and Biotherapy, University Hospital Saint Eloi, Montpellier 34295, France. isabelle.richard@inserm.fr.|University of Montpellier, Montpellier 34090, France. isabelle.richard@inserm.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Duroux-Richard
authors.affiliation: Laboratory of Nano-Regenerative Medicine, Faculty of Medicine, Universidad de Los Andes, Santiago 7620001, Chile. jcuenca@c4c.cl.|Cells for Cells, Santiago 7620001, Chile. jcuenca@c4c.cl.
authors.forename: Jimena
authors.initials: J
authors.lastname: Cuenca
authors.affiliation: INSERM, U1183, Institute of Regenerative Medicine and Biotherapy, University Hospital Saint Eloi, Montpellier 34295, France. clara.ponsolles@inserm.fr.|University of Montpellier, Montpellier 34090, France. clara.ponsolles@inserm.fr.
authors.forename: Clara
authors.initials: C
authors.lastname: Ponsolles
authors.affiliation: Laboratory of Nano-Regenerative Medicine, Faculty of Medicine, Universidad de Los Andes, Santiago 7620001, Chile. ABADILLA@uandes.cl.
authors.forename: Alejandro Badilla
authors.initials: AB
authors.lastname: Piñeiro
authors.affiliation: Laboratory of Nano-Regenerative Medicine, Faculty of Medicine, Universidad de Los Andes, Santiago 7620001, Chile. fgonzalf@uc.cl.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gonzalez
authors.affiliation: Exploratory Unit, Sanofi R & D, Montpellier 34184, France. Christine.roubert@sanofi.com.
authors.forename: Christine
authors.initials: C
authors.lastname: Roubert
authors.affiliation: Hospital Félix Bulnes, Santiago 7510021, Chile. roser.areny@gmail.com.
authors.forename: Roser
authors.initials: R
authors.lastname: Areny
authors.affiliation: Hospital Barros Luco, Santiago 8900085, Chile. rcheavi@gmail.com.
authors.forename: Rosa
authors.initials: R
authors.lastname: Chea
authors.affiliation: Hospital Barros Luco, Santiago 8900085, Chile. jacquelinepefaur@gmail.com.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Pefaur
authors.affiliation: INSERM, U1183, Institute of Regenerative Medicine and Biotherapy, University Hospital Saint Eloi, Montpellier 34295, France. ym-pers@chu-montpellier.fr.|University of Montpellier, Montpellier 34090, France. ym-pers@chu-montpellier.fr.|Clinical Department for Osteoarticular Diseases, University Hospital Lapeyronie, Montpellier 34295, France. ym-pers@chu-montpellier.fr.
authors.forename: Yves-Marie
authors.initials: YM
authors.lastname: Pers
authors.affiliation: Laboratory of Nano-Regenerative Medicine, Faculty of Medicine, Universidad de Los Andes, Santiago 7620001, Chile. ffigueroa@uandes.cl.
authors.forename: Fernando E
authors.initials: FE
authors.lastname: Figueroa
authors.affiliation: INSERM, U1183, Institute of Regenerative Medicine and Biotherapy, University Hospital Saint Eloi, Montpellier 34295, France. christian.jorgensen@inserm.fr.|University of Montpellier, Montpellier 34090, France. christian.jorgensen@inserm.fr.|Clinical Department for Osteoarticular Diseases, University Hospital Lapeyronie, Montpellier 34295, France. christian.jorgensen@inserm.fr.
authors.forename: Christian
authors.initials: C
authors.lastname: Jorgensen
authors.affiliation: Laboratory of Nano-Regenerative Medicine, Faculty of Medicine, Universidad de Los Andes, Santiago 7620001, Chile. MKhoury@uandes.cl.|Cells for Cells, Santiago 7620001, Chile. MKhoury@uandes.cl.
authors.forename: Maroun
authors.initials: M
authors.lastname: Khoury
authors.affiliation: INSERM, U1183, Institute of Regenerative Medicine and Biotherapy, University Hospital Saint Eloi, Montpellier 34295, France. florence.apparailly@inserm.fr.|University of Montpellier, Montpellier 34090, France. florence.apparailly@inserm.fr.|Clinical Department for Osteoarticular Diseases, University Hospital Lapeyronie, Montpellier 34295, France. florence.apparailly@inserm.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Apparailly
mesh_terms: D000328:Adult; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D002677:Chile; D016000:Cluster Analysis; D005602:France; D020869:Gene Expression Profiling; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology*; D008181:Lupus Nephritis / Q000175:diagnosis / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: lupus; lupus nephritis; memory B cells; microRNAs; naive B cells