title: Sequencing data and MLPA analysis data in support of the effectiveness and reliability of an asymmetric PCR-Based approach in preparing long MLPA probes.
abstract: ABI PRISM 3100 Genetic Analyzer, a multi-color fluorescence-based DNA analysis system with 16 capillaries operating in parallel, was ideal tool both for DNA sequencing and DNA fragment analysis [1,2]. To demonstrate the effectiveness and reliability of an asymmetric PCR-Based approach (X.Y. Ling, G.M. Zhang, G. Pan, H. Long, Y.H. Cheng, C.Y. Xiang, L. Kang, F. Chen, Z.N. Chen, Preparing long probes by an asymmetric PCR-based approach for multiplex ligation-dependent probe amplification (MLPA), Anal. Biochem. (2015), http://dx.doi.org/10.1016/j.ab.2015.03.031, in press) in preparing the long MLPA probes that were generated with a M13-based method before [4], some prepared long MLPA probes were sequenced and then tested in MLPA analysis. Sequencing data shows that the long MLPA probes were identical to the designed ones, indicating the long probes can be easily prepared with the new method, and the MPLA analysis data shows that the results of MPLA analysis with these long probes were as same accurate and specific as with ones prepared with other methods. The sequencing data was not presented in the research article (X.Y. Ling, G.M. Zhang, G. Pan, H. Long, Y.H. Cheng, C.Y. Xiang, L. Kang, F. Chen, Z.N. Chen, Preparing long probes by an asymmetric PCR-based approach for multiplex ligation-dependent probe amplification (MLPA), Anal. Biochem. (2015), 10.1016/j.ab.2015.03.031, in press), but the MLPA analysis data was converted into figure 4 and figure 5 of the research article.
authors.affiliation: Animal and Plant Inspection and Quarantine Technical Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, 1011 Fuqiang Road, Futian District, Shenzhen City 518045, Guangdong Province, China.
authors.forename: Xingyuan
authors.initials: X
authors.lastname: Ling
authors.affiliation: Animal and Plant Inspection and Quarantine Technical Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, 1011 Fuqiang Road, Futian District, Shenzhen City 518045, Guangdong Province, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Long
authors.affiliation: Animal and Plant Inspection and Quarantine Technical Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, 1011 Fuqiang Road, Futian District, Shenzhen City 518045, Guangdong Province, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Pan
authors.affiliation: Institute of Animal and Plant Inspection and Quarantine, Shenzhen Academy of Inspection and Quarantine, 2049 Heping Road, Luohu District, Shenzhen City 518001, Guangdong Province, China.
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Chen
keywords: ABI PRISM 3100 Genetic analyzer; DNA sequencing; MLPA analysis; MLPA probe