title: Perfluorocarbon-Encapsulated PLGA-PEG Emulsions as Enhancement Agents for Highly Efficient Reoxygenation to Cell and Organism.
abstract: Perfluorocarbon (PFC), a kind of oxygen carrier, is encapsulated in PLGA-PEG to prepare a PLGA-PEG/PFC emulsion for highly efficient reoxygenation to cell and organism. HCT 116 cells are used as a model cell, whose viability has a significant enhancement after reoxygenation with PLGA-PEG/PFC emulsion because of the sufficient and timely oxygen supply. Meanwhile, hypoxia-reoxygenation injury will happen along with cell hypoxia-reoxygenation treatment, which is reflected by increasing reactive oxygen species (ROS) in cells. However, the integration of intracellular ROS and cell viability implies that the degree of hypoxia-reoxygenation injury is sublethal to HCT116 cells when the concentration of PLGA-PEG/PFC emulsion is lower than 0.2 mg/mL. Furthermore, the change of the expression level of hypoxia-inducible factor-1α (HIF-1α) is similar to that of cell viability during reoxygenation, which suggests that HIF-1α or its downstream proteins may make a significant contribution to cell viability. In vivo oxygen supply is assessed in rats through pulmonary delivery, which shows that PLGA-PEG/PFC emulsion can supply oxygen to rats and improve rats' lung ventilation.
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Gastroenterology, ChangHai Hospital, Second Military Medical University , Shanghai 200433, P. R. China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.affiliation: National Engineering Research Center for Nanotechnology , 28 East Jiang Chuan Road, Shanghai 200241, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, ChangHai Hospital, Second Military Medical University , Shanghai 200433, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, Second Military Medical University , Shanghai 200003, P. R. China.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, Second Military Medical University , Shanghai 200003, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guan
authors.affiliation: National Engineering Research Center for Nanotechnology , 28 East Jiang Chuan Road, Shanghai 200241, P. R. China.|Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences , Shanghai 200032, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, Second Military Medical University , Shanghai 200003, P. R. China.
authors.forename: Zhaofen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , 800 Dongchuan Road, Shanghai 200240, P. R. China.|National Engineering Research Center for Nanotechnology , 28 East Jiang Chuan Road, Shanghai 200241, P. R. China.|Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences , Shanghai 200032, P. R. China.
authors.forename: Dannong
authors.initials: D
authors.lastname: He
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D004655:Emulsions / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D005434:Flow Cytometry; D005466:Fluorocarbons / Q000737:chemistry*; D045325:HCT116 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D008168:Lung / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014018:Tissue Distribution
chemical_list: D004655:Emulsions; D005466:Fluorocarbons; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017382:Reactive Oxygen Species; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D010100:Oxygen
keywords: HIF-1α; ROS; hypoxia-reoxygenation; perfluorocarbon emulsion; pulmonary delivery