title: miR-29c regulates NAV3 protein expression in a transgenic mouse model of Alzheimer's disease.
abstract: The microRNA-29 family (miRNA-29s) has three mature members, miR-29a, miR-29b and miR-29c, which have been implicated in the regulation of the pathogenesis of Alzheimer's disease (AD). The miR-29 family members exhibit differential regulation in various diseases and different subcellular distribution. In the present study, we initially investigated differential expression of miR-29c in the hippocampus and the frontal cortex of the young APPswe/PSΔE9 mouse brain, accompanied by inverse expression of neurone navigator 3 (NAV3), a regulator of axon guidance. We observed that miR-29c directly mediated downregulation of NAV3 protein expression in vitro. The mouse NAV3 mRNA has a functional miR-29c binding site in the 3' UTR, which localized in the position between 830-836 bp of 3'UTR region, slightly different from human NAV3 mRNA binding site. These observations suggest that miR-29c may be involved in neurodegenerative processes by regulating NAV3 expression in the young AD mouse.
authors.affiliation: Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zong
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
authors.forename: Pin
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
authors.forename: Chunlian
authors.initials: C
authors.lastname: Liang
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China. Electronic address: qinyejun@163.com.
authors.forename: Yejun
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China. Electronic address: qinchuan@pumc.edu.cn.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000502:physiology; D000367:Age Factors; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000473:pathology; D053764:Presenilin-1 / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D012342:RNA, Small Nuclear / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D016564:Amyloid beta-Protein Precursor; C547014:MIRN29 microRNA, mouse; D008565:Membrane Proteins; D035683:MicroRNAs; C464453:NAV3 protein, human; D009419:Nerve Tissue Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D012342:RNA, Small Nuclear; D049452:Green Fluorescent Proteins
keywords: APPswe/PSΔE9 mice; Alzheimer׳s disease; NAV3 protein; miR-29c; microRNAs