title: iTRAQ-based proteomic profiling of Vibrio parahaemolyticus under various culture conditions.
abstract: BACKGROUND
  Vibrio parahaemolyticus is a common pathogen infecting humans and marine animals; this pathogen has become a major concern of marine food products and trade. In this study, V. parahaemolyticus isolated from sewage was exposed to different culture conditions and analyzed by isobaric tag for relative and absolute quantitation (iTRAQ) based reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. Our goal is to gain further insights into the proteomics of V. parahaemolyticus, particularly differentially expressed proteins closely correlated with growth conditions and pathogenicity associated proteins.
  
  
  RESULTS
  In this study, a total of 2,717 proteins including numerous membrane proteins were significantly identified, and 616 proteins displayed significant differential expression under different conditions. Of them, 12 proteins mainly participating in metabolism showed the most elastic expression differentiation between different culture conditions. Some membrane proteins such as type I secretion outer membrane protein, TolC, lipoprotein, efflux system proteins iron-regulated protein A and putaive Fe-regulated protein B, ferric siderophore receptor homolog and several V. parahaemolyticus virulence-associated proteins were differentially regulated under different conditions. Some differentially regulated proteins were analyzed and confirmed at gene expression level by quantitative real time polymerase chain reaction (qRT-PCR).
  
  
  CONCLUSIONS
  Proteomics analysis results revealed the characteristics of V. parahaemolyticus proteome expression, provided some promising biomarkers related with growth conditions, the results likely advance insights into the mechanism involved in the response of V. parahaemolyticus to different conditions. Some virulence-associated proteins were discovered to be differentially expressed under different conditions.
authors.affiliation: School of Marine Science, Ningbo University, Ningbo, 315211 P.R. China ; College of Life Science, Liaoning Normal University, Dalian, 116081 P.R. China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Marine Science, Ningbo University, Ningbo, 315211 P.R. China.
authors.forename: Dewen
authors.initials: D
authors.lastname: Ding
authors.affiliation: School of Marine Science, Ningbo University, Ningbo, 315211 P.R. China.
authors.forename: Chundan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Marine Science, Ningbo University, Ningbo, 315211 P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Marine Science, Ningbo University, Ningbo, 315211 P.R. China.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Su
keywords: Biomarker; Pathogenicity; Quantitative proteomics; Vibrio parahaemolyticus; iTRAQ