title: Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds.
abstract: We quantified amino groups displayed on inorganic and organic surfaces in aqueous solution using different types of cleavable fluorescent compounds and an aldehyde dye. The cleavable fluorescent compounds were designed to bind covalently to amino groups and then liberated under specific conditions. Among the investigated materials, cleavable coumarin was most appropriate for the quantification of amino groups on silica and resin surfaces. The developed method can measure small amounts (∼pmol/cm(2)) of amino groups on a flat polymeric surface, detecting only amino groups that are exposed to aqueous solution and available for surface immobilization of ligands and biomolecules.
authors.affiliation: †Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Shiota
authors.affiliation: †Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: †Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Ayane
authors.initials: A
authors.lastname: Shimomura
authors.affiliation: ‡Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
authors.affiliation: †Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishino
authors.affiliation: †Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Maruyama
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000577:Amides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000447:Aldehydes; D000577:Amides; D005456:Fluorescent Dyes; D012822:Silicon Dioxide