title: Installing logic gates in permeability controllable polyelectrolyte-carbon nitride films for detecting proteases and nucleases.
abstract: Proteases and nucleases are enzymes heavily involved in many important biological processes, such as cancer initiation, progression, and metastasis; hence, they are indicative of potential diagnostic biomarkers. Here, we demonstrate a new label free and sensitive electrochemiluminescent (ECL) sensing strategy for protease and nuclease assays that utilize target-triggered desorption of programmable polyelectrolyte films assembled on graphite-like carbon nitride (g-C3N4) film to regulate the diffusion flux of a coreactant. Furthermore, we have built Boolean logic gates OR and AND into the polyelectrolyte films, capable of simultaneously sensing proteases and nucleases in a complicated system by breaking it into simple functions. The developed intelligent permeability controlled enzyme sensor may prove valuable in future medical diagnostics.
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, and College of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, China.|School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Lichan
authors.initials: L
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, and College of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Zeng
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Anirban
authors.initials: A
authors.lastname: Dandapat
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, and College of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, China.
authors.forename: Yuwu
authors.initials: Y
authors.lastname: Chi
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.|School of Chemical Engineering, Sungkyunkwan University , 16419, Republic of Korea.
authors.forename: Donghwan
authors.initials: D
authors.lastname: Kim
mesh_terms: D004247:DNA / Q000032:analysis*; D003850:Deoxyribonuclease I / Q000032:analysis* / Q000378:metabolism; D055664:Electrochemical Techniques; D004573:Electrolytes / Q000737:chemistry; D008163:Luminescent Measurements; D009570:Nitriles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014357:Trypsin / Q000032:analysis* / Q000378:metabolism
chemical_list: D004573:Electrolytes; D009570:Nitriles; D011108:Polymers; C011206:cyanogen; D004247:DNA; D003850:Deoxyribonuclease I; D014357:Trypsin