title: Prevalence of influenza A/H3N2 virus in northern Iran from 2011 to 2013.
abstract: BACKGROUND
  Influenza A virus is the most virulent human pathogen and causes the most serious problem. Having epidemiological knowledge about this disease is important. The aim of this study was to determine the prevalence of influenza A/H3N2 virus infection in northern Iran from 2011 to 2013 using the real-time polymerase chain reaction (RT-PCR).
  
  
  METHODS
  In this cross-sectional study 57 samples were collected from patients with influenza-like illness (T≥ 38 °C and cough or sore throat. Influenza-RNA was extracted from the samples using PureLink(TM) Viral RNA/DNA Kit. RT-PCR was one using SuperScript III Platinum, Quantitive Real Time PCR system from invitrogen with a specific type of primers and probs. All samples were examined in the Influenza laboratory of Mazandaran University of Medical Sciences.
  
  
  RESULTS
  The mean age of patients was 38.2±14.5 year, 278 (48.69%) were males and 293 (51.31%) females. A total number of 201 patients (35.2%) were diagnosed as influenza A1 H3 N2 infection.
  
  
  CONCLUSIONS
  The results show that the prevalence of A/H3N2 in the North of Iran is considerable and needs more attention for preventive measures.
authors.affiliation: Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Haghshenas
authors.affiliation: Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Jafarian
authors.affiliation: Antimicrobial Resistance Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Farhang
authors.initials: F
authors.lastname: Babamahmoodi
authors.affiliation: Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Tabrizi
authors.affiliation: Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Sharbano
authors.initials: S
authors.lastname: Nandoost
authors.affiliation: Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh-Navaei
keywords: Influenza A; Influenza A/H3N2; Season flu; Swine flu