title: Thrombospondin promoted anti-tumor of adenovirus-mediated calreticulin in breast cancer: Relationship with anti-CD47.
abstract: OBJECTIVE
  Calreticulin (CRT) protein has multifaceted role in carcinogenesis, however its role in breast cancer remains unidentified. In this study, we attempted to evaluate the effect of overexpressed CRT on breast cancer cells viability and proliferation.
  
  
  METHODS
  Levels of mRNA and protein expression for CRT and CD47 in cells were determined by Quantitative RT-PCR analysis and Western blot, respectively. Cells apoptosis was evaluated using Annexin V-FITC assay with flow cytometry. Cell viability was assessed using MTT assay. Cell migration and autophagy were also evaluated.
  
  
  RESULTS
  In breast cancer cells of MCF-7 and MDA-MB-231, both CRT and CD47 expression were enhanced, compared with that in normal breast cells of MCF-10A. Overexpression of CRT by MCF-7 and MDA-MB-231 cells transfected with significantly suppressed cell migration, viability as well as promote cell apoptosis while exerted no effected on cell autophagy. Interestingly, combining of thrombospondin (TSP) and overexpression of CRT significantly induced cell autophagy and inhibited tumor growth in MCF-7 cells xenograft. In result of chip assay, we observed that TSP treatment promoted interaction of TSP with CRT and CD47.
  
  
  CONCLUSIONS
  TSP promoted anti-tumor of adenovirus-mediated CRT via forming complexes with CRT and CD47 in breast cancer.
authors.affiliation: Department of General Surgery, 117th Hospital of PLA, No. 14, Road Lingyin, District Xihu, 310013 Hangzhou, PR China. Electronic address: qifeng_c@163.com.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of General Surgery, 117th Hospital of PLA, No. 14, Road Lingyin, District Xihu, 310013 Hangzhou, PR China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of General Surgery, 117th Hospital of PLA, No. 14, Road Lingyin, District Xihu, 310013 Hangzhou, PR China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, 117th Hospital of PLA, No. 14, Road Lingyin, District Xihu, 310013 Hangzhou, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yao
authors.affiliation: Department of General Surgery, 117th Hospital of PLA, No. 14, Road Lingyin, District Xihu, 310013 Hangzhou, PR China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Fang
mesh_terms: D000256:Adenoviridae; D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D051928:CD47 Antigen / Q000096:biosynthesis*; D037282:Calreticulin / Q000096:biosynthesis*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008810:Mice, Inbred C57BL; D019699:Thrombospondins / Q000494:pharmacology / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C576483:CALR protein, human; D051928:CD47 Antigen; C497894:CD47 protein, human; D037282:Calreticulin; D019699:Thrombospondins
keywords: Autophagy; CD47; Calreticulin; MCF-7; MDA-MB-231; Thrombospondin