title: Trace matrix solid phase dispersion using a molecular sieve as the sorbent for the determination of flavonoids in fruit peels by ultra-performance liquid chromatography.
abstract: A simple, rapid, and highly selective trace matrix solid phase dispersion (MSPD) technique, coupled with ultra-performance liquid chromatography-ultraviolet detection, was proposed for extracting flavonoids from orange fruit peel matrices. Molecular sieve SBA-15 was applied for the first time as a solid support in trace MSPD. Parameters, such as the type of dispersant, mass ratio of the sample to the dispersant, grinding time, and elution pH, were optimized in detail. The optimal extraction conditions involved dispersing a powdered fruit peel sample (25 mg) into 25mg of SBA-15 and then eluting the target analytes with 500 μL of methanol. A satisfactory linearity (r(2) > 0.9990) was obtained, and the calculated limits of detection reached 0.02-0.03 μg/mL for the compounds. The results showed that the method developed was successfully applied to determine the content of flavonoids in complex fruit peel matrices.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Hu
authors.affiliation: Integrated Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou 310003, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Pang
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D057230:Limit of Detection; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D005419:Flavonoids; C509969:SBA-15; D012822:Silicon Dioxide
keywords: Flavonoids; Molecular sieve; Orange fruit peel; Trace matrix solid phase dispersion; Ultra performance liquid chromatography