title: Morphological Evaluation of Oxidative Phosphorylation System in Myocardial Infarction under Conditions of Modified Vascular Endothelial Growth Factor Concentration.
abstract: The relationship between vascular endothelium growth factor (VEGF) and cardiomyocyte oxidative phosphorylation level in experimental myocardial infarction, caused by diathermocoagulation of the periconical ventricular artery, was studied by immunofluorescent microscopy. Staining showed uneven distribution of cytochrome c in the mitochondria in the focus of myocardial infarction 2 h after the operation. After 24 h uneven staining of cardiomyocytes was found in the peri-infarction zone and no staining in the zone of myocardial infarction. This indicated a significant decrease in the level of redox enzymes. This picture persisted till day 14. Intraventricular injection of VEGF to animals led to a significant increase of the immunohistochemical reaction intensity, which reached the peak by day 7. The distribution of immunohistochemical reaction products under conditions of VEGF blocking was about the same as in spontaneous postinfarction reparative restitution. Our data indicated that increase of VEGF concentration in the myocardium maintained and stimulated oxidative phosphorylation in cardiomyocytes during the postinfarction period.
authors.affiliation: Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia, pathol@inbox.ru.
authors.forename: M G
authors.initials: MG
authors.lastname: Shurygin
authors.forename: I A
authors.initials: IA
authors.lastname: Shurygina
authors.forename: O V
authors.initials: OV
authors.lastname: Kanya
authors.forename: N N
authors.initials: NN
authors.lastname: Dremina
authors.forename: E L
authors.initials: EL
authors.lastname: Lushnikova
authors.forename: R D
authors.initials: RD
authors.lastname: Nepomnyashchikh
mesh_terms: D000818:Animals; D005260:Female; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A