title: Effect of resin-modified glass-ionomer cement lining and composite layering technique on the adhesive interface of lateral wall.
abstract: Interface integrity can be maintained by setting the composite in a layering technique and using liners.
  
  
  OBJECTIVE
  The aim of this in vitro study was to verify the effect of resin-modified glass-ionomer cement (RMGIC) lining and composite layering technique on the bond strength of the dentin/resin adhesive interface of lateral walls of occlusal restorations.
  
  
  METHODS
  Occlusal cavities were prepared in 52 extracted sound human molars, randomly assigned into 4 groups: Group 2H (control) - no lining + two horizontal layers; Group 4O: no lining + four oblique layers; Group V-2H: RMGIC lining (Vitrebond) + two horizontal layers; and Group V-4O: RMGIC lining (Vitrebond) + four oblique layers. Resin composite (Filtek Z250, 3M ESPE) was placed after application of an adhesive system (Adper™ Single Bond 2, 3M ESPE) dyed with a fluorescent reagent (Rhodamine B) to allow confocal microscopy analysis. The teeth were stored in deionized water at 37oC for 24 hours before being sectioned into 0.8 mm slices. One slice of each tooth was randomly selected for Confocal Laser Scanning Microscopy (CLSM) analysis. The other slices were sectioned into 0.8 mm x 0.8 mm sticks to microtensile bond strength test (MPa). Data were analyzed by two-way ANOVA and Fisher's test. Results There was no statistical difference on bond strength among groups (p>0.05). CLSM analysis showed no significant statistical difference regarding the presence of gap at the interface dentin/resin among groups.
  
  
  CONCLUSIONS
  RMGIC lining and composite layering techniques showed no effect on the microtensile bond strength and gap formation at the adhesive interface of lateral walls of high C-factor occlusal restorations.
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Larissa Marinho
authors.initials: LM
authors.lastname: Azevedo
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Leslie Carol
authors.initials: LC
authors.lastname: Casas-Apayco
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Carlos Andres
authors.initials: CA
authors.lastname: Villavicencio Espinoza
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Maria Fidela de Lima
authors.initials: MF
authors.lastname: Navarro
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Atta
mesh_terms: D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding / Q000379:methods*; D003737:Dental Cavity Preparation / Q000379:methods; D019232:Dental Restoration Failure; D003794:Dental Restoration, Temporary / Q000379:methods; D003804:Dentin / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005899:Glass Ionomer Cements / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D018613:Microscopy, Confocal; D013314:Stress, Mechanical; D013499:Surface Properties / Q000187:drug effects; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D005899:Glass Ionomer Cements; C115612:single bond; C067790:Vitrabond; D017438:Bisphenol A-Glycidyl Methacrylate