title: Role of Exogenous Hsp72 on Liver Dysfunction during Sepsis.
abstract: This study examined the role of exogenous heat shock protein 72 (Hsp72) in reversing sepsis-induced liver dysfunction. Sepsis was induced by cecal ligation and puncture. Liver function was determined on the basis of the enzymatic activities of serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT). Apoptosis was determined using terminal deoxynucleotidyl transferase dUTP nick end labeling staining. B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3 and caspase-9, and cleaved poly (ADP-ribose) polymerase (PARP) protein expressions were analyzed using Western blotting. Results showed GOT and GPT levels increased during sepsis, and levels were restored following the administration of human recombinant Hsp72 (rhHsp72). Increased liver tissue apoptosis was observed during sepsis, and normal apoptosis resumed on rhHsp72 administration. The Bcl-2/Bax ratio, cleaved caspase-3, caspase-9, and PARP protein expressions in the liver tissues were upregulated during sepsis and normalized after rhHsp72 treatment. We conclude that, during sepsis, exogenous Hsp72 restored liver dysfunction by inhibiting apoptosis via the mitochondria-initiated caspase pathway.
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Tsen-Ni
authors.initials: TN
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Jia-Jing
authors.initials: JJ
authors.lastname: Ho
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Maw-Shung
authors.initials: MS
authors.lastname: Liu
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Tzu-Ying
authors.initials: TY
authors.lastname: Lee
authors.affiliation: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 97401, Taiwan.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lu
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chia-Jen
authors.initials: CJ
authors.lastname: Liu
authors.affiliation: Center of Teaching and Research, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung 80145, Taiwan.
authors.forename: Li-Ju
authors.initials: LJ
authors.lastname: Huang
authors.affiliation: Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Sheng-I
authors.initials: SI
authors.lastname: Lue
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Department of Pediatrics, Changhua Christian Hospital, Changhua 500, Taiwan.
authors.forename: Rei-Chen
authors.initials: RC
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D050884:HSP72 Heat-Shock Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008107:Liver Diseases / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D018805:Sepsis / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D050884:HSP72 Heat-Shock Proteins; D011994:Recombinant Proteins; D051028:bcl-2-Associated X Protein