title: Maternal Smoking History Enhances the Expression of Placental Growth Factor in Invasive Trophoblasts at Early Gestation Despite Cessation of Smoking.
abstract: Maternal smoking during early pregnancy is associated with a reduced risk for preeclampsia even after smoking cessation during pregnancy. Although the pathophysiology of preeclampsia has not been established, placental growth factor (PlGF) is believed to be a key factor. The aim of this study was to assess the effect of maternal smoking on the PlGF expression in invasive trophoblasts at early gestation. We collected villous tissues from women requesting surgical termination due to non-medical reasons at 7-8 weeks of gestation. The maternal smoking status was evaluated by measuring the serum cotinine level and patients were divided into two groups: active smokers and non-smokers. After separating invasive trophoblasts from villous tissues cultured initially under 2% O2 for 24 hours, the separated invasive trophoblasts were cultured under 2% or 8% O2 for 48 hours. The expression levels of the PlGF gene in villous tissue specimens and in invasive trophoblasts cultured after the conditions were quantified using qRT-PCR. The levels of PlGF protein in the medium were quantified using an ELISA. The gene expression level of PlGF in the villi in the active-smokers was significantly higher than that of the non-smokers. In comparison of the invasive trophoblasts under normoxia and oxygenated conditions, the ratio of PlGF gene expression and protein expression under oxygenation (2% O2+8% O2 / 2% O2+2% O2) in the active-smokers were both significantly higher than in the non-smokers. Maternal smoking history appears to stimulate PlGF expression in invasive trophoblasts under oxygenated conditions. This may be one of several causes leading to the protective effect of maternal smoking on preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kawashima
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Koide
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Hasegawa
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Arakaki
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Takenaka
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Maruyama
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Matsuoka
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism*; D016540:Smoking Cessation; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology
chemical_list: C000608036:PGF protein, human; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; D010100:Oxygen