title: Drug Delivery Strategies of Chemical CDK Inhibitors.
abstract: The pharmacological use of new therapeutics is often limited by a safe and effective drug-delivery system. In this sense, new chemical CDK inhibitors are not an exception. Nanotechnology may be able to solve some of the main problems limiting cancer treatments such as more specific delivery of therapeutics and reduction of toxic secondary effects. It provides new delivery systems able to specifically target cancer cells and release the active molecules in a controlled fashion. Specifically, silica mesoporous supports (SMPS) have emerged as an alternative for more classical drug delivery systems based on polymers. In this chapter, we describe the synthesis of a SMPS containing the CDK inhibitor roscovitine as cargo molecule and the protocols for confirmation of the proper cargo release of the nanoparticles in cell culture employing cell viability, cellular internalization, and cell death induction studies.
authors.affiliation: Centre Mediterranéen de Médecine Moléculaire (C3M), équipe "Biology and pathology of melanocyte cells: from skin pigmentation to melanomas", Institut national de la santé et de la recherche médicale (Inserm), U1065, BP 2 3194, 06204, Nice, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Alvira
authors.forename: Laura
authors.initials: L
authors.lastname: Mondragón
mesh_terms: D016923:Cell Death; D002470:Cell Survival; D002626:Chemistry, Pharmaceutical / Q000379:methods; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D016503:Drug Delivery Systems; D004353:Drug Evaluation, Preclinical; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D008422:Materials Testing; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D047428:Protein Kinase Inhibitors / Q000008:administration & dosage*; D011687:Purines / Q000008:administration & dosage*; D000077546:Roscovitine; D012822:Silicon Dioxide / Q000737:chemistry; D013501:Surface-Active Agents
chemical_list: D011108:Polymers; D047428:Protein Kinase Inhibitors; D011687:Purines; D013501:Surface-Active Agents; D000077546:Roscovitine; D012822:Silicon Dioxide; D018844:Cyclin-Dependent Kinases