title: Comparative Biochemical and Proteomic Analyses of Soybean Seed Cultivars Differing in Protein and Oil Content.
abstract: This study develops differential protein profiles of soybean (Glycine max) seeds (cv. Saedanbaek and Daewon) varying in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) content using protamine sulfate (PS) precipitation method coupled with a 2D gel electrophoresis (2DGE) approach. Of 71 detected differential spots between Daewon and Saedanbaek, 48 were successfully identified by MALDI-TOF/TOF. Gene ontology analysis revealed that up-regulated proteins in Saedanbaek were largely associated with nutrient reservoir activity (42.6%), which included mainly seed-storage proteins (SSPs; subunits of glycinin and β-conglycinin). Similar results were also obtained in two cultivars of wild soybean (G. soja cv. WS22 and WS15) differing in protein content. Western blots confirmed higher accumulation of SSPs in protein-rich Saedanbaek. Findings presented and discussed in this study highlight a possible involvement of the urea cycle for increased accumulation of SSPs and hence the higher protein content in soybean seeds.
authors.affiliation: †Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
authors.forename: Chul Woo
authors.initials: CW
authors.lastname: Min
authors.affiliation: †Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: †Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
authors.forename: So Wun
authors.initials: SW
authors.lastname: Kim
authors.affiliation: †Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
authors.forename: So Eui
authors.initials: SE
authors.lastname: Lee
authors.affiliation: †Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
authors.forename: Yong Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: ‡Center for Research Facilities, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Dong Won
authors.initials: DW
authors.lastname: Bae
authors.affiliation: §Department of Functional Crops, National Institute of Crop Science, Rural Development Administration, Miryang 627-803, South Korea.
authors.forename: Won Young
authors.initials: WY
authors.lastname: Han
authors.affiliation: §Department of Functional Crops, National Institute of Crop Science, Rural Development Administration, Miryang 627-803, South Korea.
authors.forename: Byong Won
authors.initials: BW
authors.lastname: Lee
authors.affiliation: §Department of Functional Crops, National Institute of Crop Science, Rural Development Administration, Miryang 627-803, South Korea.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Ko
authors.affiliation: ∥Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO 13265, Kathmandu 44600, Nepal.|⊥GRADE (Global Research Arch for Developing Education) Academy, Pvt. Ltd, Adarsh Nagar-13, Birgunj 44300, Nepal.
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.affiliation: ∥Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO 13265, Kathmandu 44600, Nepal.|⊥GRADE (Global Research Arch for Developing Education) Academy, Pvt. Ltd, Adarsh Nagar-13, Birgunj 44300, Nepal.
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.affiliation: †Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D010802:Phylogeny; D010938:Plant Oils / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism
chemical_list: D010938:Plant Oils; D010940:Plant Proteins
keywords: 2D gel electrophoresis; Glycine max; Glycine soja; MALDI-TOF/TOF; protamine sulfate precipitation method; seed-storage proteins