title: A Pilot Study of the Photoprotective Effects of Strawberry-Based Cosmetic Formulations on Human Dermal Fibroblasts.
abstract: Strawberry polyphenols have been extensively studied over the last two decades for their beneficial properties. Recently, their possible use in ameliorating skin conditions has also been proposed; however, their role in preventing UVA-induced damage in cosmetic formulation has not yet been investigated. Skin is constantly exposed to several environmental stressors, such as UVA radiation, that induce oxidative stress, inflammation and cell death via the production of reactive oxygen species (ROS). In the present study, we assessed the potential photoprotective capacity of different strawberry-based formulations, enriched with nanoparticles of Coenzyme Q10 and with sun protection factor 10 (SPF10), in human dermal fibroblasts (HuDe) exposed to UVA radiation. We confirmed that strawberries are a very rich source of polyphenols, anthocyanins and vitamins, and possess high total antioxidant capacity. We also showed that strawberry extracts (25 μg/mL-1 mg/mL) exert a noticeable photoprotection in HuDe, increasing cell viability in a dose-dependent way, and that these effects are potentiated by the presence of CoQ10red (100 μg/mL). We have demonstrated for the first time that the topical use of strawberry extract may provide good photoprotection, even if more in-depth studies are strongly encouraged in order to evaluate the cellular and molecular effects of strawberry protection.
authors.affiliation: Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Ancona 60131, Italy. m.gasparrini@univpm.it.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gasparrini
authors.affiliation: Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Ancona 60131, Italy. tamara.forbe@gmail.com.|Area de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI), Campeche 24040, Mexico. tamara.forbe@gmail.com.
authors.forename: Tamara Yuliett
authors.initials: TY
authors.lastname: Forbes-Hernandez
authors.affiliation: Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Ancona 60131, Italy. dolla.bihs@gmail.com.
authors.forename: Sadia
authors.initials: S
authors.lastname: Afrin
authors.affiliation: Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Ancona 60131, Italy. malvarez@unach.edu.ec.|Facultad de Ciencias de la Salud, Universidad Nacional de Chimborazo, Riobamba 060150, Ecuador. malvarez@unach.edu.ec.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Alvarez-Suarez
authors.affiliation: Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, Salamanca E37007, Spain. paramas@usal.es.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Gonzàlez-Paramàs
authors.affiliation: Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, Salamanca E37007, Spain. csb@usal.es.
authors.forename: Celestino
authors.initials: C
authors.lastname: Santos-Buelga
authors.affiliation: Dipartimento di Scienze Biomediche e Sanità Pubblica, Facoltà di Medicina, Università Politecnica delle Marche Via Ranieri 65, Ancona 60131, Italy. s.bompadre@univpm.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Bompadre
authors.affiliation: Department of Physiology, Institute of Nutrition and Food Technology \\\"José Mataix\\\", Biomedical Research Centre, University of Granada, Granada 18071, Spain. jlquiles@ugr.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Quiles
authors.affiliation: Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Ancona 60131, Italy. b.mezzetti@univpm.it.
authors.forename: Bruno
authors.initials: B
authors.lastname: Mezzetti
authors.affiliation: Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Ancona 60131, Italy. f.giampieri@univpm.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D031985:Fragaria / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013473:Sunscreening Agents / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013473:Sunscreening Agents
keywords: UVA-radiation; cell viability; human dermal fibroblasts; skin damage; strawberry polyphenols; topical formulation