title: Inhibitory effects of furan derivatives and phenolic compounds on dark hydrogen fermentation.
abstract: The inhibitory effects of furan derivatives [i.e. furfural and 5-hydroxymethylfurfural (5-HMF)] and phenolic compounds (i.e. vanillin and syringaldehyde) on dark hydrogen fermentation from glucose were comparatively evaluated. Phenolic compounds exhibited stronger inhibition on hydrogen production and glucose consumption than furan derivatives under the same 15mM concentration. Furan derivatives were completely degraded after 72h fermentation, while over 55% of phenolic compounds remained unconverted after 108h fermentation. The inhibition coefficients of vanillin (14.05) and syringaldehyde (11.21) were higher than those of 5-HMF (4.35) and furfural (0.64). Vanillin exhibited the maximum decrease of hydrogen yield (17%). The consumed reducing power by inhibitors reduction from R-CHO to RCH2OH was a possible reason contributed to the decreased hydrogen yield. Vanillin exhibited the maximum delay of peak times of hydrogen production rate and glucose consumption. Soluble metabolites and carbon conversion efficiency decreased with inhibitors addition, which were consistent with hydrogen production.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Richen
authors.initials: R
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Lingkan
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China; Department of Life Science and Engineering, Jining University, Jining 273155, China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D001547:Benzaldehydes / Q000378:metabolism*; D056804:Biofuels; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism*; D005663:Furans; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen; D010636:Phenols
chemical_list: D001547:Benzaldehydes; D056804:Biofuels; D005663:Furans; D010636:Phenols; C069665:syringaldehyde; C008046:5-hydroxymethylfurfural; D006859:Hydrogen; C100058:vanillin; D005662:Furaldehyde; D005947:Glucose; C039281:furan
keywords: Furan derivatives; Hydrogen fermentation; Inhibition; Phenolic compounds