title: Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method.
abstract: ABO genotyping is commonly used in several situations, including blood transfusion, personal identification and disease detection. The present study developed a novel method for ABO genotyping, using loop‑mediated isothermal amplification (LAMP). This method allows the simultaneous determination of six ABO genotypes under 40 min at a constant temperature of 62˚C. The genotypes of 101 blood samples were determined to be AA (n=6), AO (n=38), BB (n=12), BO (n=29), AB (n=8) and OO (n=8) by the LAMP assay. The results were compared with the phenotypes determined by serological assay and the genotypes determined by direct sequencing, and no discrepancies were observed. This novel and rapid method, with good accuracy and reasonably cost effective, provides a supplement to routine serological ABO typing and may also be useful in other point‑of‑care testing.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
authors.forename: Juanli
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Transfusion Medicine, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China.
authors.forename: Jiangcun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Biology, Providence College, Providence, RI 02918, USA.
authors.forename: Yinsheng
authors.initials: Y
authors.lastname: Wan
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D005838:Genotype; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000017:ABO Blood-Group System