title: Unsaturated fatty acids as high-affinity ligands of the C-terminal Per-ARNT-Sim domain from the Hypoxia-inducible factor 3α.
abstract: Hypoxia-inducible transcription factors (HIF) form heterodimeric complexes that mediate cell responses to hypoxia. The oxygen-dependent stability and activity of the HIF-α subunits is traditionally associated to post-translational modifications such as hydroxylation, acetylation, ubiquitination, and phosphorylation. Here we report novel evidence showing that unsaturated fatty acids are naturally occurring, non-covalent structural ligands of HIF-3α, thus providing the initial framework for exploring its exceptional role as a lipid sensor under hypoxia.
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Fala
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Juliana F
authors.initials: JF
authors.lastname: Oliveira
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Douglas
authors.initials: D
authors.lastname: Adamoski
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais,, Campinas, SP, Brazil, 13083-100.
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Aricetti
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Marilia M
authors.initials: MM
authors.lastname: Dias
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Marcio V B
authors.initials: MVB
authors.lastname: Dias
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Sforça
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Lopes-de-Oliveira
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Silvana A
authors.initials: SA
authors.lastname: Rocco
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais,, Campinas, SP, Brazil, 13083-100.|Max Plack-partner group at the Laboratório Nacional de Ciência e Tecnologia do Bioetanol.
authors.forename: Camila
authors.initials: C
authors.lastname: Caldana
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Sandra M G
authors.initials: SMG
authors.lastname: Dias
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil, 13083-100.
authors.forename: Andre L B
authors.initials: ALB
authors.lastname: Ambrosio
mesh_terms: D051017:Apoptosis Regulatory Proteins; D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008024:Ligands; D019787:Linoleic Acid / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D050178:Monoglycerides / Q000737:chemistry / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D015398:Signal Transduction; D013229:Stearic Acids / Q000737:chemistry / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; D051792:Basic Helix-Loop-Helix Proteins; D008024:Ligands; D019787:Linoleic Acid; D050178:Monoglycerides; D019301:Oleic Acid; D011994:Recombinant Proteins; D012097:Repressor Proteins; D013229:Stearic Acids; C116973:HIF3A protein, human; C031183:stearic acid