title: Klebsiella pneumoniae subsp. pneumoniae-bacteriophage combination from the caecal effluent of a healthy woman.
abstract: A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA (+)). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus "Kp36likevirus."
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.|Department of Biomedical Sciences, University of Westminster, London, United Kingdom.
authors.forename: Lesley
authors.initials: L
authors.lastname: Hoyles
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.
authors.forename: James
authors.initials: J
authors.lastname: Murphy
authors.affiliation: Max Rubner-Institut (MRI), Institute of Microbiology and Biotechnology (MBT), Kiel, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Neve
authors.affiliation: Max Rubner-Institut (MRI), Institute of Microbiology and Biotechnology (MBT), Kiel, Germany.
authors.forename: Knut J
authors.initials: KJ
authors.lastname: Heller
authors.affiliation: Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England-Colindale, London, United Kingdom.
authors.forename: Jane F
authors.initials: JF
authors.lastname: Turton
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mahony
authors.affiliation: Department of Gastroenterology, Guy's and St Thomas' NHS Foundation Trust and King's College London, London, United Kingdom.
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Sanderson
authors.affiliation: Department of Gastroenterology, Guy's and St Thomas' NHS Foundation Trust and King's College London, London, United Kingdom.
authors.forename: Barry
authors.initials: B
authors.lastname: Hudspith
authors.affiliation: Food Microbial Sciences Unit, Department of Food and Nutritional Sciences, University of Reading, Reading, Berkshire, United Kingdom.
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Gibson
authors.affiliation: Food Microbial Sciences Unit, Department of Food and Nutritional Sciences, University of Reading, Reading, Berkshire, United Kingdom.
authors.forename: Anne L
authors.initials: AL
authors.lastname: McCartney
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.|Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
keywords: Biofilm; Depolymerase; Gut microbiota; Microbial ecology; Siphoviridae