title: Minimal synthetic cells to study integrin-mediated adhesion.
abstract: To shed light on cell-adhesion-related molecular pathways, synthetic cells offer the unique advantage of a well-controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin αIIb β3 as the adhesion-mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin-specific adhesion of the engineered liposomes to fibrinogen- and fibronectin-functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin αIIb β3 .
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).|Department of Biophysical Chemistry, University of Heidelberg, INF 253, 69120 Heidelberg (Germany).
authors.forename: Johannes P
authors.initials: JP
authors.lastname: Frohnmayer
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).|Department of Biophysical Chemistry, University of Heidelberg, INF 253, 69120 Heidelberg (Germany).
authors.forename: Dorothea
authors.initials: D
authors.lastname: Brüggemann
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).|Department of Biophysical Chemistry, University of Heidelberg, INF 253, 69120 Heidelberg (Germany).
authors.forename: Christian
authors.initials: C
authors.lastname: Eberhard
authors.affiliation: Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany).
authors.forename: Stefanie
authors.initials: S
authors.lastname: Neubauer
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).|Department of Biophysical Chemistry, University of Heidelberg, INF 253, 69120 Heidelberg (Germany).|CSF Biomaterials and Cellular Biophysics, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).
authors.forename: Christine
authors.initials: C
authors.lastname: Mollenhauer
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).|Department of Biophysical Chemistry, University of Heidelberg, INF 253, 69120 Heidelberg (Germany).|CSF Biomaterials and Cellular Biophysics, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany).
authors.forename: Heike
authors.initials: H
authors.lastname: Boehm
authors.affiliation: Institute for Advanced Study (IAS) and Center of Integrated Protein Science (CIPSM), Department Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany).
authors.forename: Horst
authors.initials: H
authors.lastname: Kessler
authors.affiliation: The Weizmann Institute of Science, Department of Molecular Cell Biology, Rehovot (Israel).
authors.forename: Benjamin
authors.initials: B
authors.lastname: Geiger
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart (Germany). spatz@is.mpg.de.|Department of Biophysical Chemistry, University of Heidelberg, INF 253, 69120 Heidelberg (Germany). spatz@is.mpg.de.
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D058045:Artificial Cells / Q000737:chemistry*; D002448:Cell Adhesion; D015394:Molecular Structure; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D019039:Platelet Glycoprotein GPIIb-IIIa Complex
keywords: cell adhesion; integrin; liposomes; peptide mimetics; quartz crystal microbalance