title: Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.
abstract: The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.
authors.affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.
authors.forename: Di-Hua
authors.initials: DH
authors.lastname: Li
authors.affiliation: College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.
authors.forename: Yuan-Shan
authors.initials: YS
authors.lastname: Lv
authors.affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Liu
authors.affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Zhang
authors.affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Zhuo
mesh_terms: D005663:Furans / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D029626:Ranunculaceae / Q000737:chemistry*
chemical_list: D005663:Furans; D005960:Glucosides; C068512:liriodendrin