title: A sustainable method for effective regulation of anaerobic granular sludge: artificially increasing the concentration of signal molecules by cultivating a secreting strain.
abstract: This study introduces sustainable quorum sensing (QS) granulation for anaerobic granular sludge (AnGS) and investigates the efficiency of three types of signal molecules on regulating AnGS granulation. The signal molecules of a secreting strain cultured in a QS regulating reactor increased their concentrations in an expanded granular sludge bed reactor throughout the granulating process. Increasing content of autoinducer-2 (AI-2) strengthened interspecific QS communication and gave a best performance with larger granular diameters, higher extracellular polymeric substance (EPS) production and relative hydrophobicity (RH). N-butyryl-homoserine lactone (C4-HSL) QS regulation was also favorable for granular growth, but its regulation was less than that of AI-2-QS. The AnGS granulated under these two types of QS regulations guided more filamentous bacteria to take part in granulation. Under diffusible signal factor (DSF)-QS regulation, the sludge had a lower granular level with a smaller granule diameter, lower EPS production (RH) when compared that of control medium.
authors.affiliation: Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou 310012, China.
authors.forename: Yangcheng
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou 310012, China. Electronic address: fenghuajun@mail.zjgsu.edu.cn.
authors.forename: Huajun
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Wenkun
authors.initials: W
authors.lastname: Huang
authors.affiliation: Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou 310012, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Shen
authors.affiliation: Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Zhejiang Gongshang University, Hangzhou 310012, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Wang
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D001419:Bacteria; D019149:Bioreactors; D046508:Culture Techniques; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D007783:Lactones; D053038:Quorum Sensing / Q000502:physiology*; D012722:Sewage / Q000382:microbiology
chemical_list: D007783:Lactones; C092312:N-butyrylhomoserine lactone; C091088:N-octanoylhomoserine lactone; D012722:Sewage; D006714:Homoserine; D015107:4-Butyrolactone
keywords: Anaerobic granular sludge; Granulation; Quorum sensing; Signal molecules; Sustainable regulation