title: The Arabidopsis ortholog of the DEAH-box ATPase Prp16 influences auxin-mediated development.
abstract: In animals and yeasts, the DEAH-box RNA-dependent ATPase Prp16 facilitates pre-mRNA splicing. However, in Chlamydomonas reinhardtii and Caenorhabditis elegans, Prp16 orthologs are not important for general pre-mRNA splicing, but are required for gene silencing and sex determination, respectively. The CLUMSY VEIN (CUV) gene, which encodes a unique Prp16 ortholog in Arabidopsis thaliana, influences auxin-mediated development. A loss-of-function cuv-1 mutation tells us that CUV does not facilitate splicing of pre-mRNA substrates indiscriminately, but differentially effects splicing and expression of genes. Here we show that CUV influences root-meristem maintenance and planar polarity of root-hair positioning, both of which are processes regulated by auxin. We propose that Arabidopsis PRP16/CUV differentially facilitates the expression of genes, including genes involved in auxin biosynthesis, transport, perception and signaling, and that in this way it influences auxin-mediated development.
authors.affiliation: a Department of Botany ; Graduate School of Science; Kyoto University ; Sakyo-ku, Kyoto , Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Tsugeki
authors.affiliation: a Department of Botany ; Graduate School of Science; Kyoto University ; Sakyo-ku, Kyoto , Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Terada
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D018519:Meristem; D009154:Mutation; D063245:Plant Development / Q000235:genetics*; D018517:Plant Roots; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D012322:RNA Precursors; D000251:Adenosine Triphosphatases; C027900:RNA-dependent ATPase; D053487:DEAD-box RNA Helicases
keywords: Arabidopsis; CLUMSY VEIN; Prp16; auxin; planar polarity; pre-mRNA splicing; root hair