title: Antioxidant and enzymatic responses to oxidative stress induced by pre-harvest water supply reduction and ripening on mango (Mangifera indica L. cv. 'Cogshall') in relation to carotenoid content.
abstract: The effects of a reduction in water supply during fruit development and postharvest fruit ripening on the oxidative status and the antioxidant defense system were studied in the mango fruit (Mangifera indica L.) cv. Cogshall. Changes in non-enzymatic (ascorbate) and enzymatic (SOD, CAT, APX, MDHAR, DHAR and GR) antioxidants, as well as oxidative parameters (H2O2 and MDA) and major carotenoids, were measured in unripe and ripe fruits from well-irrigated and non-irrigated trees. Under non-limiting water supply conditions, ripening induced oxidation as a result of the production of ROS and decreased ascorbate content. Antioxidant enzymatic systems were activated to protect fruit tissues and to regenerate the ascorbate pool. The carotenoid pool, mainly represented by β-carotene and esterified violaxanthine isomers, accumulated naturally during mango ripening. The suppression of irrigation decreased fruit size and induced accumulation of ABA and of its storage form, ABA-GE, in fruit pulp from the earliest harvest. It also increased oxidation, which was observable by the high levels of ascorbate measured at the early stages at harvest, and by the delay in the time it took to reach the pseudo constant carotene-to-xanthophyll ratio in ripe fruits. Nevertheless, differences between the irrigation treatments on the antioxidant system in ripe fruits were not significant, mainly because of the drastic changes in this system during ripening.
authors.affiliation: Centre de Coopération Internationale de Recherche Agronomique pour le Développement (CIRAD), UMR Qualisud, 7 Rue de l'Irat, 97410 Saint-Pierre, Ile de La Réunion, France; Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Faculté des Sciences et Technologies, Université de La Réunion, Sainte-Clotilde, Ile de la Réunion, France. Electronic address: rosalie.remy@gmail.com.
authors.forename: Rémy
authors.initials: R
authors.lastname: Rosalie
authors.affiliation: CIRAD, UMR Qualisud, TA B95/16, 73 rue Jean-François Breton, 34398, Montpellier Cedex 5, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Joas
authors.affiliation: Centre de Coopération Internationale de Recherche Agronomique pour le Développement (CIRAD), UMR Qualisud, 7 Rue de l'Irat, 97410 Saint-Pierre, Ile de La Réunion, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Deytieux-Belleau
authors.affiliation: Centre de Coopération Internationale de Recherche Agronomique pour le Développement (CIRAD), UMR Qualisud, 7 Rue de l'Irat, 97410 Saint-Pierre, Ile de La Réunion, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Vulcain
authors.affiliation: Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Faculté des Sciences et Technologies, Université de La Réunion, Sainte-Clotilde, Ile de la Réunion, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Payet
authors.affiliation: Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Université de La Réunion, ESIROI Agroalimentaire, Parc Technologique, 2 rue Joseph Wetzell, 97490, Sainte-Clotilde, Ile de la Réunion, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Dufossé
authors.affiliation: CIRAD, UR Fonctionnement Agroécologique et Performances des Systèmes de Culture Horticoles, Station Bassin Plat, BP 180, 97455, Saint Pierre Cedex, Ile de la Réunion, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Léchaudel
mesh_terms: D057914:Agricultural Irrigation; D000975:Antioxidants / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D031022:Mangifera / Q000201:enzymology / Q000378:metabolism*; D018384:Oxidative Stress; D014867:Water / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D014867:Water; D002338:Carotenoids
keywords: Abscisic acid; Ascorbate; Carotenoids; Irrigation; Mango; ROS; Ripening