title: Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus.
abstract: The genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. The type species, S. moniliformis, is one of the two etiological agents of rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field isolates and reference strains of S. moniliformis, S. hongkongensis, S. felis as well as divergent isolates were characterized by comparison of molecular data (n = 29) and from the majority also by their physiological as well as proteomic properties (n = 22). Based on growth-independent physiological profiling using VITEK2-compact, API ZYM and the Micronaut system fastidious growth-related difficulties could be overcome and streptobacilli could definitively be typed despite generally few differences. While differing in their isolation sites and dates, S. moniliformis isolates were found to possess almost identical spectra in matrix-assisted laser desorption ionization-time of flight mass spectrometry and Fourier transform infrared spectroscopy. Spectroscopic methods facilitated differentiation of S. moniliformis, S. hongkongensis and S. felis as well as one divergent isolate. Sequencing of 16S rRNA gene as well as functional genes groEL, recA and gyrB revealed only little intraspecific variability, but generally proved suitable for interspecies discrimination between all three taxa and two groups of divergent isolates.
authors.affiliation: Hessian State Laboratory, Department of Veterinary Medicine, Giessen, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.affiliation: German Cancer Research Center, Heidelberg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Nicklas
authors.affiliation: Chemical and Veterinary Investigation Office (CVUA) Stuttgart, Fellbach, Germany.
authors.forename: Norman
authors.initials: N
authors.lastname: Mauder
authors.affiliation: Chemical and Veterinary Investigation Office (CVUA) Stuttgart, Fellbach, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Rau
authors.affiliation: Chemical and Veterinary Investigation Office (CVUA) Stuttgart, Fellbach, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Contzen
authors.affiliation: Robert Koch Institute, Berlin, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Semmler
authors.affiliation: Institute for Multiphase Processes, Leibniz University, Hannover, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Hofmann
authors.affiliation: Merlin Diagnostika, Bornheim-Hersel, Germany.
authors.forename: Khayrieh
authors.initials: K
authors.lastname: Aledelbi
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Giessen, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Ewers
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001483:Base Sequence; D002645:Chickens; D016002:Discriminant Analysis; D005838:Genotype; D006801:Humans; D016013:Likelihood Functions; D051379:Mice; D010641:Phenotype; D010802:Phylogeny; D051381:Rats; D013288:Streptobacillus / Q000166:cytology* / Q000235:genetics* / Q000302:isolation & purification