title: High organic loading treatment for industrial molasses wastewater and microbial community shifts corresponding to system development.
abstract: Molasses wastewater contains high levels of organic compounds, cations, and anions, causing operational problems for anaerobic biological treatment. To establish a high organic loading treatment system for industrial molasses wastewater, this study designed a combined system comprising an acidification tank, a thermophilic multi-stage (MS)-upflow anaerobic sludge blanket (UASB) reactor, mesophilic UASB reactor, and down-flow hanging sponge reactor. The average total chemical oxygen demand (COD) and biochemical oxygen demand removal rates were 85%±3% and 95%±2%, respectively, at an organic loading rate of 42kgCODcrm(-3)d(-1) in the MS-UASB reactor. By installation of the acidification tank, the MS-UASB reactor achieved low H2-partial pressure. The abundance of syntrophs such as fatty acid-degrading bacteria increased in the MS-UASB and 2nd-UASB reactors. Thus, the acidification tank contributed to maintaining a favorable environment for syntrophic associations. This study provides new information regarding microbial community composition in a molasses wastewater treatment system.
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Kyohei
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Chosei
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Nakahara
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Hatamoto
authors.affiliation: Mitsui Sugar Co., Ltd., 36-2, Nihonbashi-Hokazakicho, Chuo-ku, Tokyo 103-8423, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Wakabayashi
authors.affiliation: Mitsui Sugar Co., Ltd., 36-2, Nihonbashi-Hokazakicho, Chuo-ku, Tokyo 103-8423, Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Department of Civil Engineering, Nagaoka National College of Technology, 888, Nishi-Katakai, Nagaoka, Niigata 940-8532, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Araki
authors.affiliation: Center for Regional Environmental Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Syutsubo
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan. Electronic address: ecoya@vos.nagaokaut.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D059013:Microbial Consortia; D008965:Molasses; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: 16S rRNA gene sequencing; Down-flow hanging sponge; High organic loading treatment; True industrial molasses wastewater; Upflow anaerobic sludge blanket