title: Different surface sensing of the cell body and nucleus in healthy primary cells and in a cancerous cell line on nanogrooves.
abstract: Cancer cells are known to have alterations compared to healthy cells, but can these differences extend to the way cells interact with their environment? Here, the authors focused on the alignment on an array of grooves of nanometer depth using two cell types: healthy osteoprogenitor primary cells (HOP) and a cancerous osteosarcoma (SaOs-2) cell line. Another concern was how this alignment affects the cell's interior, namely, the nucleus. Based on the results, it is proposed that these two cell types respond to different size regimes: SaOs-2 cells are more sensitive to shallow grooves while HOP cells are strongly aligned with deep grooves. As a measure of the impact of cell alignment on the nucleus the orientation and elongation of the nucleus were determined. Compared to HOP cells, the cell nucleus of SaOs-2 cells is more aligned and elongated in response to grooves, suggesting a softer nucleus and/or increased force transmission. These results support the hypothesis that cancer cells have reduced nucleus rigidity compared to healthy ones and further indicate differences in sensing, which may be important during metastasis.
authors.affiliation: Institut de Science des Matériaux de Mulhouse, CNRS UMR7361, Université de Haute-Alsace, Mulhouse 68057, France.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Davidson
authors.affiliation: LAMIH, Mechanical Department, CNRS UMR 8201, Université de Valenciennes et du Hainaut Cambrésis, Valenciennes 59313, France.
authors.forename: Maxence
authors.initials: M
authors.lastname: Bigerelle
authors.affiliation: Institute of Physics, Faculty of Mathematics and Physics, Albert-Ludwigs-University of Freiburg, Freiburg 79104, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Reiter
authors.affiliation: Institut de Science des Matériaux de Mulhouse, CNRS UMR7361, Université de Haute-Alsace, Mulhouse 68057, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
mesh_terms: D002448:Cell Adhesion; D002467:Cell Nucleus / Q000502:physiology*; D048430:Cell Shape; D006801:Humans; D013234:Stem Cells / Q000502:physiology*; D013499:Surface Properties; D014407:Tumor Cells, Cultured / Q000502:physiology*