title: Transgene integration into the human AAVS1 locus enhances myosin II-dependent contractile force by reducing expression of myosin binding subunit 85.
abstract: The adeno-associated virus site 1 (AAVS1) locus in the human genome is a strong candidate for gene therapy by insertion of an exogenous gene into the locus. The AAVS1 locus includes the coding region for myosin binding subunit 85 (MBS85). Although the function of MBS85 is not well understood, myosin II-dependent contractile force may be affected by altered expression of MBS85. The effect of altered expression of MBS85 on cellular contractile force should be examined prior to the application of gene therapy. In this study, we show that transgene integration into AAVS1 and consequent reduction of MBS85 expression changes myosin II-dependent cellular contractile force. We established a human fibroblast cell line with exogenous DNA knocked-in to AAVS1 (KI cells) using the CRISPR/Cas9 genome editing system. Western blotting analysis showed that KI cells had significantly reduced MBS85 expression. KI cells also showed greater cellular contractile force than control cells. The increased contractile force was associated with phosphorylation of the myosin II regulatory light chain (MRLC). Transfection of KI cells with an MBS85 expression plasmid restored cellular contractile force and phosphorylation of MRLC to the levels in control cells. These data suggest that transgene integration into the human AAVS1 locus induces an increase in cellular contractile force and thus should be considered as a gene therapy to effect changes in cellular contractile force.
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan. Electronic address: mizutani@sci.hokudai.ac.jp.
authors.forename: Takeomi
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Haga
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kawabata
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D000076987:CRISPR-Associated Protein 9; D060846:Cell Engineering; D002460:Cell Line; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D000229:Dependovirus / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter; D056426:Genetic Loci; D015894:Genome, Human; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009119:Muscle Contraction / Q000235:genetics; D018994:Myosin Light Chains / Q000235:genetics / Q000378:metabolism*; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D054645:Protein Phosphatase 1 / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
chemical_list: D001426:Bacterial Proteins; D018994:Myosin Light Chains; D049452:Green Fluorescent Proteins; D000076987:CRISPR-Associated Protein 9; C000600049:Cas9 protein, Francisella novicida; D004720:Endonucleases; C540874:PPP1R12C protein, human; D054645:Protein Phosphatase 1; D024462:Myosin Type II
keywords: Adeno-associated virus site 1; Cellular contractile force; Genome editing; Myosin binding subunit 85; Myosin regulatory light chain phosphorylation