title: In vivo and in vitro effect of hepatocarcinoma lymph node metastasis by upregulation of Annexin A7 and relevant mechanisms.
abstract: We unveiled the association of Annexin A7 with vascular endothelial growth factor-C (VEGF-C) and the effect of upregulation of Annexin A7 in Hca-F and Hca-P cells on inhibiting hepatocarcinoma (HCC) lymph node metastasis (LNM) in vitro and in vivo. A total of 200 inbred 615 mice were randomly divided into four equal groups inoculated with Hca-F, Hca-P, FAnxa7-upregulated, and PAnxa7-upregulated cells, respectively. The primary tumor, popliteal, inguinal, and iliac lymph nodes were prepared for immunohistochemical (IHC) staining, real-time quantitative polymerase chain reaction (qRT-PCR) analysis, Western blot, and hematoxylin-eosin (H&E) staining. There was over 50 % increase both in the number of FAnxa7-upregulated and PAnxa7-upregulated cells migrated through the filter compared to their controls (FAnxa7-control, Hca-F and PAnxa7-control, Hca-P). However, no significant differences were noted in invasion ability between them (all P > 0.05). Tumor lymph vessels were significantly reduced in FAnxa7-upregulated and PAnxa7-upregulated tumors when compared with Hca-F and Hca-P tumors (all P < 0.05). Blood vessel density did not differ significantly between FAnxa7-upregulated and PAnxa7-upregulated tumors and Hca-F and Hca-P tumors. Enzyme-linked immunosorbent assay (ELISA) for VEGF-C showed that upregulating Annexin A7 decreased VEGF-C secretion in FAnxa7-upregulated and PAnxa7-upregulated cells (P < 0.05). The IHC staining result showed that the level of serum Annexin A7 was found to be statistically higher in all experimental groups than that in the control group (P < 0.05). The present results indicated that alterations in serum Annexin A7 expression may be of prognostic relevance in HCC lymphatic metastasis.
authors.affiliation: Department of Pathology, Qiqihar Medical University, Qiqihar, 161006, People's Republic of China.
authors.forename: Xian-Yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Anesthesia, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161006, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Pathology, Qiqihar Medical University, Qiqihar, 161006, People's Republic of China.
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, No. 9, West Lvshun Southern Road, Dalian, 116044, People's Republic of China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Bai
authors.affiliation: Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, No. 9, West Lvshun Southern Road, Dalian, 116044, People's Republic of China.
authors.forename: Mohammed Mohammed
authors.initials: MM
authors.lastname: Ibrahim
authors.affiliation: Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, No. 9, West Lvshun Southern Road, Dalian, 116044, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tumor Metastasis of Liaoning Province, Department of Pathology, Dalian Medical University, No. 9, West Lvshun Southern Road, Dalian, 116044, People's Republic of China. jwtang_53@sina.cn.
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: Tang
mesh_terms: D000818:Animals; D017310:Annexin A7 / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D015854:Up-Regulation; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism
chemical_list: D017310:Annexin A7; C480504:Anxa7 protein, mouse; D042582:Vascular Endothelial Growth Factor C
keywords: Annexin A7; Hepatocarcinoma; Lymph node metastasis; Vascular endothelial growth factor-C