title: Inactivation of Staphylococcus aureus in Oat and Soya Drinks by Enterocin AS-48 in Combination with Other Antimicrobials.
abstract: The presence of toxicogenic Staphylococcus aureus in foods and the dissemination of methicillin-resistant S. aureus (MRSA) in the food chain are matters of concern. In the present study, the circular bacteriocin enterocin AS-48, applied singly or in combination with phenolic compounds (carvacrol, eugenol, geraniol, and citral) or with 2-nitro-1-propanol (2NPOH), was investigated in the control of a cocktail made from 1 methicillin-sensitive and 1 MRSA strains inoculated on commercial oat and soya drinks. Enterocin AS-48 exhibited low bactericidal activity against staphylococci in the drinks investigated when applied singly. The combinations of sub-inhibitory concentrations of enterocin AS-48 (25 μg/mL) and phenolic compounds or 2NPOH caused complete inactivation of staphylococci in the drinks within 24 h of incubation at 22 °C. When tested in oat and soya drinks stored for 7 d at 10 °C, enterocin AS-48 (25 μg/mL) in combination with 2NPOH (5.5 mM) reduced viable counts rapidly in the case of oat drink (4.2 log cycles after 12 h) or slowly in soya drink (3.8 log cycles after 3 d). The same combined treatment applied on drinks stored at 22 °C achieved a fast inactivation of staphylococci within 12 to 24 h in both drinks, and no viable staphylococci were detected for up to 7 d of storage. Results from the study highlight the potential of enterocin AS-48 in combination with 2NPOH for inactivation of staphylococci.
authors.affiliation: Dept. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, Jaén, 23071, Spain.
authors.forename: María José Grande
authors.initials: MJ
authors.lastname: Burgos
authors.affiliation: Dept. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, Jaén, 23071, Spain.
authors.forename: M Carmen López
authors.initials: MC
authors.lastname: Aguayo
authors.affiliation: Dept. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, Jaén, 23071, Spain.
authors.forename: Rubén Pérez
authors.initials: RP
authors.lastname: Pulido
authors.affiliation: Dept. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, Jaén, 23071, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gálvez
authors.affiliation: Dept. de Ciencias de la Salud, Facultad de Ciencias Experimentales, Univ. de Jaén, Jaén, 23071, Spain.
authors.forename: Rosario Lucas
authors.initials: RL
authors.lastname: López
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D018554:Avena / Q000382:microbiology*; D001430:Bacteriocins / Q000494:pharmacology*; D001628:Beverages / Q000382:microbiology*; D015169:Colony Count, Microbial; D004032:Diet; D005054:Eugenol / Q000494:pharmacology; D005516:Food Microbiology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D009574:Nitro Compounds / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D020005:Propanols / Q000494:pharmacology; D045730:Soy Foods / Q000382:microbiology; D013025:Glycine max / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013729:Terpenes / Q000494:pharmacology
chemical_list: C496417:2-nitropropanol; D000890:Anti-Infective Agents; D001430:Bacteriocins; D009574:Nitro Compounds; D010636:Phenols; D020005:Propanols; D013729:Terpenes; C107799:enterocin A; D005054:Eugenol
keywords: Staphylococcus; cereal drinks; enterocin