title: Interaction of bovine serum albumin with N-acyl amino acid based anionic surfactants: Effect of head-group hydrophobicity.
abstract: The function of a protein depends upon its structure and surfactant molecules are known to alter protein structure. For this reason protein-surfactant interaction is important in biological, pharmaceutical, and cosmetic industries. In the present work, interactions of a series of anionic surfactants having the same hydrocarbon chain length, but different amino acid head group, such as l-alanine, l-valine, l-leucine, and l-phenylalanine with the transport protein, bovine serum albumin (BSA), were studied at low surfactant concentrations using fluorescence and circular dichroism (CD) spectroscopy, and isothermal titration calorimetry (ITC). The results of fluorescence measurements suggest that the surfactant molecules bind simultaneously to the drug binding site I and II of the protein subdomain IIA and IIIA, respectively. The fluorescence as well as CD spectra suggest that the conformation of BSA goes to a more structured state upon surfactant binding at low concentrations. The binding constants of the surfactants were determined by the use of fluorescence as well as ITC measurements and were compared with that of the corresponding glycine-derived surfactant. The binding constant values clearly indicate a significant head-group effect on the BSA-surfactant interaction and the interaction is mainly hydrophobic in nature.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India.
authors.forename: Subhajit
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India. Electronic address: joydey@chem.iitkgp.ernet.in.
authors.forename: Joykrishna
authors.initials: J
authors.lastname: Dey
mesh_terms: D000215:Acylation; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D005453:Fluorescence; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D000596:Amino Acids; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine
keywords: Anionic surfactants; BSA; Binding; Calorimetry; Circular dichroism; Fluorescence