title: Phospholipase Dε enhances Braasca napus growth and seed production in response to nitrogen availability.
abstract: Phospholipase D (PLD), which hydrolyses phospholipids to produce phosphatidic acid, has been implicated in plant response to macronutrient availability in Arabidopsis. This study investigated the effect of increased PLDε expression on nitrogen utilization in Brassica napus to explore the application of PLDε manipulation to crop improvement. In addition, changes in membrane lipid species in response to nitrogen availability were determined in the oil seed crop. Multiple PLDε over expression (PLDε-OE) lines displayed enhanced biomass accumulation under nitrogen-deficient and nitrogen-replete conditions. PLDε-OE plants in the field produced more seeds than wild-type plants but have no impact on seed oil content. Compared with wild-type plants, PLDε-OE plants were enhanced in nitrate transporter expression, uptake and reduction, whereas the activity of nitrite reductase was higher under nitrogen-depleted, but not at nitrogen-replete conditions. The level of nitrogen altered membrane glycerolipid metabolism, with greater impacts on young than mature leaves. The data indicate increased expression of PLDε has the potential to improve crop plant growth and production under nitrogen-depleted and nitrogen-replete conditions.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Lu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Shuaibing
authors.initials: S
authors.lastname: Yao
authors.affiliation: Department of Biology, University of Missouri, St. Louis, MO, USA.|Donald Danforth Plant Science Center, St. Louis, MO, USA.
authors.forename: Geliang
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Hong
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.|Department of Biology, University of Missouri, St. Louis, MO, USA.|Donald Danforth Plant Science Center, St. Louis, MO, USA.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology*; D018533:Biomass; D029688:Brassica napus / Q000187:drug effects / Q000254:growth & development*; D002734:Chlorophyll / Q000378:metabolism; D000069599:Crop Production / Q000379:methods; D005227:Fatty Acids / Q000378:metabolism; D035264:Flowers / Q000187:drug effects / Q000502:physiology; D006017:Glycolipids / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000494:pharmacology*; D010739:Phospholipase D / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*
chemical_list: D000872:Anthocyanins; D005227:Fatty Acids; D006017:Glycolipids; D008563:Membrane Lipids; D009566:Nitrates; D010938:Plant Oils; D002734:Chlorophyll; D010739:Phospholipase D; D009584:Nitrogen
keywords: Brassica napus; biomass; lipids; nitrogen; phospholipase Dε