title: Investigating Cellular Responses During Photohydrogen Production by the Marine Microalga Tetraselmis subcordiformis by Quantitative Proteome Analysis.
abstract: The marine microalga Tetraselmis subcordiformis could photoproduce hydrogen under the regulation of carbonyl cyanide m-chlorophenylhydrazone (CCCP), and a hydrogen production process kinetic analysis was characterized by two peaks, suggesting that two distinct mechanisms might exist in this alga. Therefore, 2D nanoliquid chromatography-tandem mass spectrometry (LC-MS/MS) was introduced to analyze the proteome of samples from different time points. A total of 912 proteins were identified, providing a global view of the cellular responses at the proteomic level. These proteins can be divided into multiple functional groups including stress responses, energy metabolism and redox homeostasis. The quantitative proteomic data provided more details on the electron donors for hydrogen production. During the first stage, photosystem II produced electrons for hydrogen production; during the second stage, metabolites were the major electron donors via nonphotochemical plastoquinone reduction by NADH dehydrogenase.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.|National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
authors.forename: Chaofan
authors.initials: C
authors.lastname: Ji
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Novel Technology and Materials for Separation and Detection of Biomolecules Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Biology and Pharmacy, Zhejiang Pharmaceutical College, 315100, Ningbo, People's Republic of China.
authors.forename: Junge
authors.initials: J
authors.lastname: Qu
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Yao
authors.affiliation: Novel Technology and Materials for Separation and Detection of Biomolecules Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China.
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China. xuesong@dicp.ac.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
mesh_terms: D000460:Chlorophyta / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D004579:Electron Transport / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D007700:Kinetics; D008027:Light; D053858:Metabolic Networks and Pathways / Q000528:radiation effects; D058086:Microalgae / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D040901:Proteomics
chemical_list: D006859:Hydrogen; D006864:Hydrogenase
keywords: Marine microalgae; Photohydrogen production; Quantitative proteomics; Tetraselmis subcordiformis