title: Difference in microchip electrophoretic mobility between partially and fully PEGylated poly(amidoamine) dendrimers.
abstract: The objective of this study was to investigate the difference in electrophoretic mobility between partially and fully poly(ethylene glycol)-conjugated poly(amidoamine) dendrimers (part-PEG-PAMAM and full-PEG-PAMAM, respectively) using a microchip capillary gel electrophoresis (MCGE). While MCGE allowed size-based separation of PEG-PAMAMs prepared with monomethoxy PEG-nitrophenyl carbonate, full-PEG-PAMAMs migrated slower than part-PEG-PAMAMs that were similar in size or larger. When the measured molecular weights obtained from MCGE analysis and the calculated molecular weights were plotted, each part-PEG-PAMAM and full-PEG-PAMAM showed correlation coefficients greater than 0.98. This study indicates that MCGE would be useful for characterizing PEG-PAMAMs with different PEGylation degrees.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Park
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, Republic of Korea. Electronic address: dhna@knu.ac.kr.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Na
mesh_terms: D001672:Biocompatible Materials; D050091:Dendrimers / Q000032:analysis* / Q000737:chemistry; D004337:Drug Carriers; D019075:Electrophoresis, Capillary; D046209:Electrophoresis, Microchip; D008970:Molecular Weight; D011073:Polyamines / Q000032:analysis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry; D056910:Republic of Korea; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D004337:Drug Carriers; C531249:Poly(amidoamine); D011073:Polyamines; D011092:Polyethylene Glycols
keywords: Microchip capillary gel electrophoresis; PEGylation; Poly(amidoamine) dendrimers