title: Identification of novel peptides for horse meat speciation in highly processed foodstuffs.
abstract: There is a need for robust analytical methods to support enforcement of food labelling legislation. Proteomics is emerging as a complementary methodology to existing tools such as DNA and antibody-based techniques. Here we describe the development of a proteomics strategy for the determination of meat species in highly processed foods. A database of specific peptides for nine relevant animal species was used to enable semi-targeted species determination. This principle was tested for horse meat speciation, and a range of horse-specific peptides were identified as heat stable marker peptides for the detection of low levels of horse meat in mixtures with other species.
authors.affiliation: a Food Quality and Safety Programme, Fera Science Ltd , Sand Hutton, York , UK.
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Claydon
authors.affiliation: a Food Quality and Safety Programme, Fera Science Ltd , Sand Hutton, York , UK.
authors.forename: Helen H
authors.initials: HH
authors.lastname: Grundy
authors.affiliation: a Food Quality and Safety Programme, Fera Science Ltd , Sand Hutton, York , UK.
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Charlton
authors.affiliation: a Food Quality and Safety Programme, Fera Science Ltd , Sand Hutton, York , UK.
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Romero
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D057140:Fast Foods / Q000032:analysis; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D006736:Horses; D006801:Humans; D008460:Meat / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis*; D010449:Peptide Mapping; D059748:Proteolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry
chemical_list: D009124:Muscle Proteins; D010446:Peptide Fragments; D014357:Trypsin
keywords: biomarker peptide; horse meat; mass spectrometry; meat authenticity