title: Evaluation of antileishmanial, antibacterial and brine shrimp cytotoxic potential of crude methanolic extract of Herb Ocimum basilicum (Lamiacea).
abstract: OBJECTIVE
  To collect and screen for ethnopharmacological properties (antileishmanial, antibacterial and brine lethality assays) of medicinal plan Ocimum basilicum from Peshawar region (34.008 latitude and 71.57 altitudes).
  
  
  METHODS
  In the present study a general antileishmanial activity against Leishmania tropica strair was carried out. The antibacterial potential of the plant was performed against 06 gram positiv and 06 gram negative bacteria. Brine shrimp cyto- toxicity assay at different concentrations were investigated.
  
  
  RESULTS
  The anti-promastigotes profile of the plant showed good antileishmanial activity exhibited LC50 value 21.67 µg/mL. The result for gram positive antibacterial activity revealed that the O. basilicum leaves extract possesses significant inhibitory activity at highest two concentrations ranging from 20.66 ± 0.31 to 31.86 ± 0.80 for Clostridium perfringens type C and Bacillus subtitilis, respectively, as compared to the gentamycin (27.36 ± 0.55 and 21.80 ± 0.72, respectively). For gram negative bacteria good activity was observed. A highest zone of inhibition was recorded for Pseudomonas aeroginosa (28.83 ± 0.28) at the highest concentration (10 mg/ mL). The LC50 value obtained for brine shrimp lethality assay was 91.56 µg/mL.
  
  
  CONCLUSIONS
  The herb basil possesses effective cidal activities which make this plant a good candidate for the isolation of antiprotozoal and antibacterial compounds which may lead to the development of novel drug.
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.forename: Kafeel
authors.initials: K
authors.lastname: Ahmad
authors.forename: Ali Talha
authors.initials: AT
authors.lastname: Khalil
authors.forename: Jangrez
authors.initials: J
authors.lastname: Khan
authors.forename: Yusra Ali
authors.initials: YA
authors.lastname: Khan
authors.forename: Muhammad Shahab
authors.initials: MS
authors.lastname: Saqib
authors.forename: Muhamad Naveed
authors.initials: MN
authors.lastname: Umar
authors.forename: Hilal
authors.initials: H
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001156:Artemia / Q000187:drug effects*; D001419:Bacteria / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D007891:Leishmania / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D018649:Ocimum basilicum / Q000737:chemistry*; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D000981:Antiprotozoal Agents; D010936:Plant Extracts