title: Prospective separation and transcriptome analyses of cortical projection neurons and interneurons based on lineage tracing by Tbr2 (Eomes)-GFP/Dcx-mRFP reporters.
abstract: In the cerebral cortex, projection neurons and interneurons work coordinately to establish neural networks for normal cortical functions. While the specific mechanisms that control productions of projection neurons and interneurons are beginning to be revealed, a global characterization of the molecular differences between these two neuron types is crucial for a more comprehensive understanding of their developmental specifications and functions. In this study, using lineage tracing power of combining Tbr2(Eomes)-GFP and Dcx-mRFP reporter mice, we prospectively separated intermediate progenitor cell (IPC)-derived neurons (IPNs) from non-IPC-derived neurons (non-IPNs) of the embryonic cerebral cortex. Molecular characterizations revealed that IPNs and non-IPNs were enriched with projection neurons and interneurons, respectively. Expression profiling documented cell-specific genes including differentially expressed transcriptional regulators that might be involved in cellular specifications, for instance, our data found that SOX1 and SOX2, which were known for important functions in neural stem/progenitor cells, continued to be expressed by interneurons but not by projection neurons. Transcriptome analyses of cortical neurons isolated at different stages of neurogenesis revealed distinct temporal patterns of expression of genes involved in early-born or late-born neuron specification. These data present a resource useful for further investigation of the molecular regulations and functions of projection neurons and interneurons.
authors.affiliation: Department of Neurosciences, Beckman Research Institute of City of Hope, Duarte, California, 91010.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, Duarte, California, 91010.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Neurosciences, Beckman Research Institute of City of Hope, Duarte, California, 91010.
authors.forename: Heying
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurosciences, Beckman Research Institute of City of Hope, Duarte, California, 91010.
authors.forename: Runxiang
authors.initials: R
authors.lastname: Qiu
authors.affiliation: Laboratory of Regulation of Neuronal Development, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Department of Neurosciences, Beckman Research Institute of City of Hope, Duarte, California, 91010.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002540:Cerebral Cortex / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D055748:SOXB1 Transcription Factors; D020825:T-Box Domain Proteins; D000097573:Red Fluorescent Protein; C000714847:Dcx protein, mouse; C491198:Eomes protein, mouse; C098693:Sox1 protein, mouse; C527783:Sox2 protein, mouse
keywords: cerebral cortex; interneurons; projection neurons; transcriptome