title: Alginate-hyaluronan composite hydrogels accelerate wound healing process.
abstract: In this paper we propose polysaccharide hydrogels combining alginate (ALG) and hyaluronan (HA) as biofunctional platform for dermal wound repair. Hydrogels produced by internal gelation were homogeneous and easy to handle. Rheological evaluation of gelation kinetics of ALG/HA mixtures at different ratios allowed understanding the HA effect on ALG cross-linking process. Disk-shaped hydrogels, at different ALG/HA ratio, were characterized for morphology, homogeneity and mechanical properties. Results suggest that, although the presence of HA does significantly slow down gelation kinetics, the concentration of cross-links reached at the end of gelation is scarcely affected. The in vitro activity of ALG/HA dressings was tested on adipose derived multipotent adult stem cells (Ad-MSC) and an immortalized keratinocyte cell line (HaCaT). Hydrogels did not interfere with cell viability in both cells lines, but significantly promoted gap closure in a scratch assay at early (1 day) and late (5 days) stages as compared to hydrogels made of ALG alone (p<0.01 and 0.001 for Ad-MSC and HaCaT, respectively). In vivo wound healing studies, conducted on a rat model of excised wound indicated that after 5 days ALG/HA hydrogels significantly promoted wound closure as compared to ALG ones (p<0.001). Overall results demonstrate that the integration of HA in a physically cross-linked ALG hydrogel can be a versatile strategy to promote wound healing that can be easily translated in a clinical setting.
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Catanzano
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: V
authors.initials: V
authors.lastname: D'Esposito
authors.affiliation: Department of Engineering, University of Sannio, Piazza Roma 21, 82100 Benevento, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Acierno
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: M R
authors.initials: MR
authors.lastname: Ambrosio
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: De Caro
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Avagliano
authors.affiliation: Institute for Polymers, Composites and Biomaterials, National Research Council, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy.
authors.forename: P
authors.initials: P
authors.lastname: Russo
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Russo
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Miro
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ungaro
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Calignano
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Formisano
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy. Electronic address: Quaglia@unina.it.
authors.forename: F
authors.initials: F
authors.lastname: Quaglia
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004195:Disease Models, Animal; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D020100:Hydrogels / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D017208:Rats, Wistar; D012212:Rheology / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D006820:Hyaluronic Acid
keywords: Alginate; Hyaluronan; Internal gelation; Wound dressing