title: In Vivo Healing Effects of Ankaferd Blood Stopper on the Residual Pancreatic Tissue in a Swine Model of Distal Pancreatectomy.
abstract: The aim of this study was to determine whether intraoperative Ankaferd blood stopper (ABS) application into the pancreatic channel and to the pancreatic remnant surface following distal pancreatectomy can or cannot prevent postoperative pancreatic fistula formation. Three pigs underwent distal pancreatectomy under general anesthesia. In two of the pigs, 0.5 ml of ABS was applied to the stump surface area after adding 0.5 ml of ABS into the pancreatic channel. The remaining one animal served as the control. The pigs were sacrificed on the seventh postoperative day for autopsy. The pancreatic remnants from the animals were then taken for histopathological analyses. It was observed that the oral intake had been broken and abdominal distention had developed in the control pig following on the third postoperative day. However, no significant clinical changes were observed in the ABS-applied pigs. In the autopsy, it was found that the control pig had generalized peritonitis with pancreatic necrosis. On the other hand, the ABS-applied pigs had either macroscopically and microscopically normal pancreatic tissue architecture with an occluded Wirsung duct at the pancreatic stump. It was concluded that application of ABS on the transected surface and into the pancreatic channel could prevent pancreatic fistula formation and improve wound healing in the residual pancreatic tissue following distal pancreatectomy.
authors.affiliation: Department of Gastroenterological Surgery, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey.
authors.forename: Kerem
authors.initials: K
authors.lastname: Karaman
authors.affiliation: Department of Gastroenterological Surgery, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey.
authors.forename: Erdal Birol
authors.initials: EB
authors.lastname: Bostanci
authors.affiliation: Department of Gastroenterological Surgery, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey.
authors.forename: Bahadir
authors.initials: B
authors.lastname: Celep
authors.affiliation: Ataturk Teaching and Research Hospital, Department of Pathology, Ankara, Turkey.
authors.forename: Nazmiye
authors.initials: N
authors.lastname: Dincer
authors.affiliation: Department of Gastroenterology, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Kızılay Sk. No. 2, Sıhhiye, 06100 Ankara, Turkey.
authors.forename: Mevlut
authors.initials: M
authors.lastname: Kurt
authors.affiliation: Department of Gastroenterological Surgery, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Teke
authors.affiliation: Department of Gastroenterological Surgery, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey.
authors.forename: Musa
authors.initials: M
authors.lastname: Akoglu
authors.affiliation: Department of Surgery, Ankara University, Faculty of Veterinary Medicine, Ankara, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Bilgili
authors.affiliation: Department of Surgery, Ankara University, Faculty of Veterinary Medicine, Ankara, Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Ulusan
authors.affiliation: Department of Hematology, Hacettepe University, Faculty of Medicine, Ankara, Turkey.
authors.forename: Ibrahim C
authors.initials: IC
authors.lastname: Haznedaroglu
keywords: Amylase; Ankaferd blood stopper; Distal pancreatectomy; Histopathology; Pancreatic fistula; Wound healing