title: Biomek Cell Workstation: A Variable System for Automated Cell Cultivation.
abstract: Automated cell cultivation is an important tool for simplifying routine laboratory work. Automated methods are independent of skill levels and daily constitution of laboratory staff in combination with a constant quality and performance of the methods. The Biomek Cell Workstation was configured as a flexible and compatible system. The modified Biomek Cell Workstation enables the cultivation of adherent and suspension cells. Until now, no commercially available systems enabled the automated handling of both types of cells in one system. In particular, the automated cultivation of suspension cells in this form has not been published. The cell counts and viabilities were nonsignificantly decreased for cells cultivated in AutoFlasks in automated handling. The proliferation of manual and automated bioscreening by the WST-1 assay showed a nonsignificant lower proliferation of automatically disseminated cells associated with a mostly lower standard error. The disseminated suspension cell lines showed different pronounced proliferations in descending order, starting with Jurkat cells followed by SEM, Molt4, and RS4 cells having the lowest proliferation. In this respect, we successfully disseminated and screened suspension cells in an automated way. The automated cultivation and dissemination of a variety of suspension cells can replace the manual method.
authors.affiliation: Center for Life Science Automation (celisca), University of Rostock, Germany ricarda.lehmann@celisca.de.
authors.forename: R
authors.initials: R
authors.lastname: Lehmann
authors.affiliation: Center for Life Science Automation (celisca), University of Rostock, Germany.
authors.forename: J C
authors.initials: JC
authors.lastname: Severitt
authors.affiliation: Center for Life Science Automation (celisca), University of Rostock, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Roddelkopf
authors.affiliation: Institute of Automation, University of Rostock, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Junginger
authors.affiliation: Center for Life Science Automation (celisca), University of Rostock, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Thurow
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans
keywords: Biomek Cell Workstation; automated cell culturing; life science automation; suspension cells