title: Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration.
abstract: Development of biodegradable composites having the ability to suppress or eliminate the pathogenic micro-biota or modulate the inflammatory response has attracted great interest in order to limit/repair periodontal tissue destruction. The present report includes the development of non-steroidal anti-inflammatory drug encapsulated novel biodegradable chitosan (CS)/poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) electro-spun (e-spun) composite nanofibrous mats and films and study of the effect of heat treatment on fibers and films morphology. It also describes comparative in-vitro drug release profiles from heat treated and control (non-heat treated) nanofibrous mats and films containing varying concentrations of piroxicam (PX). Electrospinning was used to obtain drug loaded ultrafine fibrous mats. The physical/chemical interactions were evaluated by Fourier Transform Infrared (FT-IR) spectroscopy. The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM). The thermal behavior of the materials was investigated by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC). Control (not heat treated) and heat treated e-spun fibers mats and films were tested for in vitro drug release studies at physiological pH7.4 and initially, as per requirement burst release patterns were observed from both fibers and films and later sustained release profiles were noted. In vitro cytocompatibility was performed using VERO cell line of epithelial cells and all the synthesized materials were found to be non-cytotoxic. The current observations suggested that these materials are potential candidates for periodontal regeneration.
authors.affiliation: Department of Chemistry, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan; Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan.
authors.forename: Ariba
authors.initials: A
authors.lastname: Farooq
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan. Electronic address: drmyar@ciitlahore.edu.pk.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yar
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan.
authors.forename: Abdul Samad
authors.initials: AS
authors.lastname: Khan
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan.
authors.forename: Lubna
authors.initials: L
authors.lastname: Shahzadi
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan.
authors.forename: Saadat Anwar
authors.initials: SA
authors.lastname: Siddiqi
authors.affiliation: Department of Allied Health Sciences and Chemical Pathology, University of Health Sciences, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Nasir
authors.initials: N
authors.lastname: Mahmood
authors.affiliation: Department of Chemistry, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Rauf
authors.affiliation: Veterinary Research Institute, Lahore, Pakistan.
authors.forename: Zafar-ul-Ahsan
authors.initials: ZU
authors.lastname: Qureshi
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan.
authors.forename: Faisal
authors.initials: F
authors.lastname: Manzoor
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan.
authors.forename: Aqif Anwar
authors.initials: AA
authors.lastname: Chaudhry
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore,54000, Pakistan; Department of Materials Science and Engineering, The Kroto Research Institute, The University of Sheffield, North Campus, Broad Lane, Sheffield S3 7HQ, United Kingdom.
authors.forename: Ihtesham
authors.initials: I
authors.lastname: ur Rehman
mesh_terms: D000818:Animals; D063566:Biodegradable Plastics / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D002522:Chlorocebus aethiops; D017886:Durapatite / Q000737:chemistry; D004847:Epithelial Cells / Q000187:drug effects; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry; D010519:Periodontium / Q000187:drug effects*; D010894:Piroxicam / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012038:Regeneration / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D063566:Biodegradable Plastics; D010894:Piroxicam; D011142:Polyvinyl Alcohol; D048271:Chitosan; D017886:Durapatite
keywords: Chitosan; Drug release; NSAIDs; Periodontal regeneration; VERO cells