title: Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange.
abstract: The genetic variability and evolution of parietaria mottle virus (PMoV) of the genus Ilarvirus was studied by analyzing nucleotide sequences of 2b and CP genes from isolates collected in different countries. Phylogenetic analysis showed that PMoV isolates clustered in different clades: one (clade I) composed of only Italian isolates and three clades (clades II-IV) including the Spanish isolates. The Greek isolate GrT-1 used in this study was in clade IV for the CP phylogenetic tree whereas it formed a separate branch in the 2b phylogenetic tree. The nucleotide sequence diversity of both the 2b and CP genes was low (0.062 ± 0.006 and 0.063 ± 0.006 for 2b and CP, respectively) but higher than those of other ilarviruses. Distribution of synonymous and nonsynonymous substitutions revealed that 2b and CP proteins are under purifying selection, with some positions under diversifying selection. Genetic exchange among Spanish isolates was also detected.
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113, Moncada, Valencia, Spain. galipienso_lui@gva.es.|Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139, Palermo, Italy. galipienso_lui@gva.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Galipienso
authors.affiliation: Programa de Biología y Biotecnología Vegetal, Universidad Autónoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Martínez
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Valencia, Spain.
authors.forename: Anouk
authors.initials: A
authors.lastname: Willemsen
authors.affiliation: Grupo de Virología, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia (UPV), 46022, Valencia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Alfaro-Férnandez
authors.affiliation: Grupo de Virología, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia (UPV), 46022, Valencia, Spain.
authors.forename: Isabel Font-San
authors.initials: IF
authors.lastname: Ambrosio
authors.affiliation: Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139, Palermo, Italy.|Department of Agricultural and Forestry Science, University of Palermo, 90128, Palermo, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Davino
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113, Moncada, Valencia, Spain.|Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139, Palermo, Italy.
authors.forename: Luis
authors.initials: L
authors.lastname: Rubio
mesh_terms: D005075:Biological Evolution; D036022:Capsid Proteins / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D019179:Ilarvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D032403:Parietaria / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*
chemical_list: D036022:Capsid Proteins