title: Microarray-based optimization to detect genomic deletion mutations.
abstract: We performed array comparative genome hybridization (aCGH) analyses of fiveArabidopsis thalianamutants with genomic deletions ranging in size from 4 bp to > 5 kb. We used the Roche NimbleGenArabidopsisCGH 3 × 720 K whole genome custom tiling array to optimize deletion detection. Details of the microarray design and hybridization data have been deposited at the NCBI GEO repository with accession number GSE55327.
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Belfield
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors.forename: Carly
authors.initials: C
authors.lastname: Brown
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK ; Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK ; Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg, Cologne 50829, Germany.
authors.forename: Xiangchao
authors.initials: X
authors.lastname: Gan
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors.forename: Caifu
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
authors.forename: Dilair
authors.initials: D
authors.lastname: Baban
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK ; Department of Biology, LUMS School of Science and Engineering, Sector UDHA, Lahore 54792, Pakistan.
authors.forename: Aziz
authors.initials: A
authors.lastname: Mithani
authors.affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Mott
authors.affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK ; McGill University and Genome Quebec Innovation Centre, 740 DR Penfield Ave., Montreal H3A 0G1, Canada.
authors.forename: Jiannis
authors.initials: J
authors.lastname: Ragoussis
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Harberd
keywords: CGH; Deletion; Genome; Microarray; Mutation