title: Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach.
abstract: Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line OSCC-BD induced by B(a)P/DMBA mixture and transformed from dysplastic oral leukoplakia cell line DOK. Cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of OSCC-BD cells. We further identified the differential proteins between DOK and OSCC-BD cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with RSD < 8%. Differential proteins are mainly related to cell cycle, cell proliferation, DNA replication, RNA splicing and apoptosis. Abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of OLK. Of the 34 identified differential proteins with RSD < 8%, 13 novel cancer-related proteins were reported in the present study. This study might provide a new insight into the mechanism of OLK malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research.
authors.affiliation: 1] College of Stomatology, Dalian Medical University, Dalian 116044, China [2] State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: 1] College of Stomatology, Dalian Medical University, Dalian 116044, China [2] State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Guowu
authors.initials: G
authors.lastname: Ma
authors.affiliation: 1] College of Stomatology, Dalian Medical University, Dalian 116044, China [2] State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Zhuwen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: 1] College of Stomatology, Dalian Medical University, Dalian 116044, China [2] State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University, Dalian 116044, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: 1] College of Stomatology, Dalian Medical University, Dalian 116044, China [2] State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Yanguang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University, Dalian 116044, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Shao
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Dong
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Xiandong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Engineering Mechanics, Dalian University of Technology, Dalian 116023, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Pathology, Cancer Hospital and Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xue
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Qimin
authors.initials: Q
authors.lastname: Zhan
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D017209:Apoptosis; D001564:Benzo(a)pyrene / Q000633:toxicity; D014408:Biomarkers, Tumor; D002273:Carcinogens / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D005786:Gene Expression Regulation; D006801:Humans; D007972:Leukoplakia, Oral / Q000503:physiopathology*; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D002273:Carcinogens; D009363:Neoplasm Proteins; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene