title: Development of Desolvation System for Single-cell Analysis Using Droplet Injection Inductively Coupled Plasma Atomic Emission Spectroscopy.
abstract: With a view to enhance the sensitivity of analytical instruments used in the measurement of trace elements contained in a single cell, we have now equipped the previously reported micro-droplet injection system (M-DIS) with a desolvation system. This modified M-DIS was coupled to inductively coupled plasma atomic emission spectroscopy (ICP-AES) and evaluated for its ability to measure trace elements. A flow rate of 100 mL/min for the additional gas and a measurement point -7.5 mm above the load coil (ALC) have been determined to be the optimal parameters for recording the emission intensity of the Ca(II) spectral lines. To evaluate the influence of the desolvation system, we recorded the emission intensities of the Ca(I), Ca(II), and H-β spectral lines with and without inclusion of the desolvation system. The emission intensity of the H-β spectral line reduces and the magnitude of the Ca(II)/Ca(I) emission intensity ratio increases four-fold with inclusion of the desolvation system. Finally, the elements Ca, Mg, and Fe present in a single cell of Pseudococcomyxa simplex are simultaneously determined by coupling the M-DIS equipped with the desolvation system to ICP-AES.
authors.affiliation: Department of Energy Sciences, Tokyo Institute of Technology.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Mari
authors.initials: M
authors.lastname: Aida
authors.forename: Akito
authors.initials: A
authors.lastname: Nomura
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Miyahara
authors.forename: Akiko
authors.initials: A
authors.lastname: Hokura
authors.forename: Akitoshi
authors.initials: A
authors.lastname: Okino
mesh_terms: D053000:Analytic Sample Preparation Methods; D000460:Chlorophyta / Q000166:cytology; D058626:Plasma Gases / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014131:Trace Elements / Q000032:analysis*
chemical_list: D058626:Plasma Gases; D012997:Solvents; D014131:Trace Elements