title: MiR-141 Inhibits Gastric Cancer Proliferation by Interacting with Long Noncoding RNA MEG3 and Down-Regulating E2F3 Expression.
abstract: BACKGROUND
  MiR-141 and long noncoding RNA MEG3 have been independently reported to be tumor suppressor genes in various cancers. However, their expression has never been previously associated with gastric cancer (GC).
  
  
  OBJECTIVE
  To investigate the interaction of miR-141 and MEG3 in GC.
  
  
  METHODS
  QRT-PCR was used to detect miR-141, MEG3, and E2F3 in gastric tissues and cells. CCK-8 and flow cytometry analysis were used to detect cell functions. Western blot and luciferase activity were used to identify E2F3 as one of the direct targets of miR-141.
  
  
  RESULTS
  We found that expression of both miR-141 and MEG3 was significantly reduced in GC compared with levels in matched nonmalignant tissues. Positive correlation between miR-141 and MEG3 was found in both tumor tissues and control tissues. Furthermore, the over-expression of either miR-141 or MEG3 in 7901 and MKN45 cells inhibited cell proliferation and cell cycle progression and promoted cell apoptosis. E2F3 was identified as a target of miR-141, and its inhibition significantly reduced MEG3 expression. E2F3 expression was also found to be negatively associated with both MEG3 and miR-141. E2F3 over-expression partly reversed the changes caused by transfection of miR-141 mimic, and inhibition of miR-141 or MEG3 overrides MEG3- or miR-141-induced modulation of cell growth in GC.
  
  
  CONCLUSIONS
  These findings together suggested that miR-141 could be interacting with MEG3 and targeting E2F3, and these factors may play important anti-tumor effects in GC pathogenesis and provide therapeutic targets in the clinics.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.|First Clinical Medical College of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.|First Clinical Medical College of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Ji
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, Shanxi, China.
authors.forename: Xiquan
authors.initials: X
authors.lastname: Ke
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Gu
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.|First Clinical Medical College of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Wujuan
authors.initials: W
authors.lastname: Jin
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. 15850668395@163.com.|First Clinical Medical College of Nanjing Medical University, Nanjing, 210029, China. 15850668395@163.com.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D016022:Case-Control Studies; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D050699:E2F3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014162:Transfection
chemical_list: D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; C498907:MEG3 non-coding RNA, human; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: Cell cycle; Cell growth; Gastric cancer; MEG3; MiR-141