title: DNA Sequence Variants in the Five Prime Untranslated Region of the Cyclooxygenase-2 Gene Are Commonly Found in Healthy Dogs and Gray Wolves.
abstract: The aim of this study was to investigate the frequency of regional DNA variants upstream to the translation initiation site of the canine Cyclooxygenase-2 (Cox-2) gene in healthy dogs. Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy. A role for Cox-2 DNA variants in genetic predisposition to canine renal dysplasia has been proposed and dog breeders have been encouraged to select against these DNA variants. We sequenced 272-422 bases in 152 dogs unaffected by renal dysplasia and found 19 different haplotypes including 11 genetic variants which had not been described previously. We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs. Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.
authors.affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Noa
authors.initials: N
authors.lastname: Safra
authors.affiliation: Animal Health Trust, Newmarket, United Kingdom.
authors.forename: Louisa J
authors.initials: LJ
authors.lastname: Hayward
authors.affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Miriam
authors.initials: M
authors.lastname: Aguilar
authors.affiliation: Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California Davis, Davis, California United States of America.
authors.forename: Benjamin N
authors.initials: BN
authors.lastname: Sacks
authors.affiliation: Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Jodi L
authors.initials: JL
authors.lastname: Westropp
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: F Charles
authors.initials: FC
authors.lastname: Mohr
authors.affiliation: Animal Health Trust, Newmarket, United Kingdom.
authors.forename: Cathryn S
authors.initials: CS
authors.lastname: Mellersh
authors.affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Danika L
authors.initials: DL
authors.lastname: Bannasch
mesh_terms: D020121:5' Untranslated Regions; D000483:Alleles; D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D008969:Molecular Sequence Data; D019596:Wolves / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D051546:Cyclooxygenase 2