title: [EPITOPIC SPECIFICITY OF A SYNTHETIC DISACCHARIDE, RECURRING LINK OF CAPSULE POLYSACCHARIDE CHAIN OF SEROTYPE 3 STREPTOCCUS PNEUMONIAE].
abstract: OBJECTIVE
  Study epitopic specificity of synthetic disaccharide, recurring link of serotype 3 S. pneumoniae, conjugated with bovine serum albumin (BSA).
  
  
  METHODS
  Conjugate of the synthetic disaccharide with BSA was obtained by squarate method. Antigenic activity of the conjugate was studied in competitive EIA. Titers of IgG against capsule polysaccharide of serotype 3 S. pneumoniae were determined in EIA by using sera of mice immunized twice with disaccharide conjugate sorbed onto aluminum hydroxide.
  
  
  RESULTS
  Disaccharide conjugate used as a well-covering antigen (4 µg/well) in EIA was characterized by a high degree of specificity and interacted only with IgG against serotype 3 S. pneumoniae in antimicrobial sera of animals without reacting with antibodies (ABs) against other pneumococcus serotypes (6B, 10A, 19A, 19F, 23F). Disaccharide conjugated with BSA was determined in competitive EIA to inhibit bonding of ABs to disaccharide by 78.8%, bacterial capsule polysaccharide by 56.9%, BSA did not inhibit the sera activity. The study of sera of mice immunized by serotype 3 S. pneumoniae disaccharide conjugate in EIA, where capsule polysaccharide was used as a plate-sorbed antigen, has established the presence of IgG against capsule polysaccharide at a titer of 1:1600.
  
  
  CONCLUSIONS
  The disaccharide that is a single recurring link of serotype 3 S. pneumoniae contains a key epitope of capsule polysaccharide. The synthetic disaccharide could be used as a component of multivalent conjugated pneumococcal vaccines and for development of diagnostic test-systems.
authors.forename: E A
authors.initials: EA
authors.lastname: Kurbatova
authors.forename: N K
authors.initials: NK
authors.lastname: Akhmatova
authors.forename: N B
authors.initials: NB
authors.lastname: Egorova
authors.forename: I B
authors.initials: IB
authors.lastname: Semenova
authors.forename: N E
authors.initials: NE
authors.lastname: Yastrebova
authors.forename: Yu E
authors.initials: YE
authors.lastname: Tsvetkov
authors.forename: E V
authors.initials: EV
authors.lastname: Sukhova
authors.forename: D V
authors.initials: DV
authors.lastname: Yashunsky
authors.forename: N E
authors.initials: NE
authors.lastname: Nifantiev
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology; D002417:Cattle; D004187:Disaccharides / Q000138:chemical synthesis / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D051379:Mice; D011008:Pneumococcal Infections / Q000276:immunology* / Q000517:prevention & control; D012680:Sensitivity and Specificity; D065288:Serogroup; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000472:pathogenicity; D014611:Vaccination; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D004187:Disaccharides; D000939:Epitopes; D018074:Vaccines, Conjugate; D012710:Serum Albumin, Bovine