title: Elutriation for Cell Cycle Synchronization in Fission Yeast.
abstract: Cell synchronization is a powerful technique for studying the eukaryotic cell cycle events precisely. The fission yeast is a rod-shaped cell whose growth is coordinated with the cell cycle. Monitoring the cellular growth of fission yeast is a relatively simple way to measure the cell cycle stage of a cell. Here, we describe a detailed method of unperturbed cell synchronization, named centrifugal elutriation, for fission yeast.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Japan, kume513@hiroshima-u.ac.jp.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kume
mesh_terms: D002452:Cell Count; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D002498:Centrifugation / Q000295:instrumentation / Q000379:methods*; D012568:Schizosaccharomyces / Q000166:cytology*; D013242:Sterilization