title: S100, bcl2 and myeloperoxid protein expirations during periodontal inflammation.
abstract: BACKGROUND
  Periodontal inflammation is characterized by injuries in collagen, epithelial, bone tissues. The hypotheses to be tested were relationship between the s100, bcl2 and myeloperoxidase in gingival tissues (MPO does affect the level of s100, bcl2). The object of this study was to investigate of s100 expression, bcl2 expression and myeloperoxidase expression in periodontal inflammation.
  
  
  METHODS
  27 patients (giant-cell epulis) and 30 patients (acute and chronic inflammations) were included in the study for s100 expression, bcl2 expression and myeloperoxidase expression by immunohistochemistry and hematoxylin--eosin.
  
  
  RESULTS
  Giant-cells in epulis positivity for myeloperoxidase has been observed in 100% However, only 75.31% of giant-cells were positive for bcl2 expression. Acute 98.2%, and chronic 89.28% inflammation was a significant positive for myeloperoxidase. The immunohistochemical findings of s100, bcl 2 and myeloperoxidase in epithelial layers have showed the result of 100%, 82,2%, 100% positive cells in acute and 100%, 78.25%, 100% in chronic process of inflammation respectively.
  
  
  CONCLUSIONS
  The results indicate that the pathogenesis of periodontal inflammation might involve inhibition of cell death, through the overexpression of bcl-2, due to identifying factors myeloperoxidase (result in the DNA damage by the product of catalysis). The highest levels of s100 activity have been found at sites with chronic inflammation.
authors.affiliation: Department of Pathological Anatomy, Sumy State University, Sumy, Ukraine. kuzenko_yevhen@rambler.ru.
authors.forename: Yevhen
authors.initials: Y
authors.lastname: Kuzenko
authors.affiliation: Department of Pathological Anatomy, Sumy State University, Sumy, Ukraine. anatoliy_rom@mail.ru.
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Romanyuk
authors.affiliation: Head of the Department of Therapeutic Dentistry, Kiev University UANM, Kyiv, Ukraine. politun@ukr.net.
authors.forename: Antonina
authors.initials: A
authors.lastname: Politun
authors.affiliation: Department of Pathological Anatomy, Sumy State University, Sumy, Ukraine. karpenko@i.ua.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Karpenko
mesh_terms: D000328:Adult; D000368:Aged; D055113:Chronic Periodontitis / Q000378:metabolism; D004396:Coloring Agents; D004848:Epithelium / Q000737:chemistry; D005260:Female; D005456:Fluorescent Dyes; D015726:Giant Cells / Q000737:chemistry; D005882:Gingival Diseases / Q000378:metabolism; D006101:Granuloma, Giant Cell / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000737:chemistry; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*; D009195:Peroxidase / Q000032:analysis*; D010950:Plasma Cells / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D019253:Proto-Oncogene Proteins c-bcl-2; D009418:S100 Proteins; D009195:Peroxidase