title: Gene expression profile of zeitlupe/lov kelch protein1 T-DNA insertion mutants in Arabidopsis thaliana: Downregulation of auxin-inducible genes in hypocotyls.
abstract: Elongation of hypocotyl cells has been studied as a model for elucidating the contribution of cellular expansion to plant organ growth. ZEITLUPE (ZTL) or LOV KELCH PROTEIN1 (LKP1) is a positive regulator of warmth-induced hypocotyl elongation under white light in Arabidopsis, although the molecular mechanisms by which it promotes hypocotyl cell elongation remain unknown. Microarray analysis showed that 134 genes were upregulated and 204 genes including 15 auxin-inducible genes were downregulated in the seedlings of 2 ztl T-DNA insertion mutants grown under warm conditions with continuous white light. Application of a polar auxin transport inhibitor, an auxin antagonist or an auxin biosynthesis inhibitor inhibited hypocotyl elongation of control seedlings to the level observed with the ztl mutant. Our data suggest the involvement of auxin and auxin-inducible genes in ZTL-mediated hypocotyl elongation.
authors.affiliation: a Department of Life Science ; Faculty of Science; Gakushuin University ; Tokyo , Japan.|b These authors contributed equally to this work.
authors.forename: Aya
authors.initials: A
authors.lastname: Saitoh
authors.affiliation: a Department of Life Science ; Faculty of Science; Gakushuin University ; Tokyo , Japan.|b These authors contributed equally to this work.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Takase
authors.affiliation: a Department of Life Science ; Faculty of Science; Gakushuin University ; Tokyo , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kitaki
authors.affiliation: a Department of Life Science ; Faculty of Science; Gakushuin University ; Tokyo , Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyazaki
authors.affiliation: a Department of Life Science ; Faculty of Science; Gakushuin University ; Tokyo , Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kiyosue
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D016254:Mutagenesis, Insertional / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D007210:Indoleacetic Acids; C036483:T-DNA; C410503:ZTL protein, Arabidopsis
keywords: Arabidopsis thaliana; SAUR; ZEITLUPE; auxin; high temperature; hypocotyl elongation; microarray