title: Enriched autologous facial fat grafts in aesthetic surgery: 3D volumetric results.
abstract: BACKGROUND
  Improved results with aesthetic fat augmentation of the face have been recently described by the concomitant use of autologous stem cells from the stromal vascular fraction (SVF).
  
  
  OBJECTIVE
  There are no studies in the literature regarding facial fat augmentation results with the use of SVF using 3D computer volumetric analyses. This prospective study was thus undertaken to answer this question.
  
  
  METHODS
  Fat was harvested by a standard liposuction technique for reinjection. A 50 cc aliquot of fat was also processed to obtain the SVF using a standard collagenase technique. A cell count was done using a cytometer, and the amount of injected fat and cells were recorded. The Vultus 3D photogrammetric scanning system was used to scan the face pre- and posttreatment and long-term, and volume changes were then calculated at the different time intervals. The data was then correlated to the variables.
  
  
  RESULTS
  Ten subjects were included in the study, with an average follow-up of 12.6 months. The average amount of fat injected was 18.4 cc, of which 68% was retained. The average cell count of the SVF was 4.8 × 105. The amount of retained fat by volume was found to be positively correlated to the number of cells in the SVF. There was no correlation between the age and number of cells in the SVF.
  
  
  CONCLUSIONS
  There is a correlation between the number of cells in the SVF and the amount of fat retained.
  
  
  METHODS
  3 Therapeutic.
authors.affiliation: Dr Schendel is Professor Emeritus of Surgery and Adjunct Clinical Professor of Neurosurgery, Stanford University Medical Center, Stanford, CA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Schendel
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000328:Adult; D004954:Esthetics; D005145:Face / Q000000981:diagnostic imaging* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D021621:Imaging, Three-Dimensional; D007271:Injections, Intradermal; D015187:Lipectomy / Q000379:methods; D008875:Middle Aged; D017060:Patient Satisfaction / Q000706:statistics & numerical data*; D011859:Radiography; D012060:Rejuvenation; D018570:Risk Assessment; D012494:Sampling Studies; D013518:Surgery, Plastic / Q000379:methods*; D020858:Tissue and Organ Harvesting / Q000379:methods; D014182:Transplantation, Autologous / Q000009:adverse effects / Q000379:methods; D016896:Treatment Outcome