title: Complete genome sequence of Methylophilus sp. TWE2 isolated from methane oxidation enrichment culture of tap-water.
abstract: The non-methane-utilizing methylotroph, Methylophilus sp. TWE2, was isolated from tap-water during the enrichment of methanotrophs with methane. The complete genome sequence of strain TWE2 showed that this bacterium may convert methanol to formaldehyde via catalysis of methanol dehydrogenase (MDH), after which formaldehyde would be assimilated to biomass through the ribulose monophosphate (RuMP) pathway or dissimilated via the tetrahydromethanopterin (H4MPT) pathway. The deficiency of glycolysis and the TCA cycle indicate that strain TWE2 may be an obligate methylotroph. This is the first complete genome sequence of the genus Methylophilus.
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Department of Ecology and Evolutionary Biology, School of Life Sciences,Fudan University, Shanghai, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xia
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zou
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Gao
authors.affiliation: Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China. Electronic address: quanzx@fudan.edu.cn.
authors.forename: Zhe-Xue
authors.initials: ZX
authors.lastname: Quan
mesh_terms: D001483:Base Sequence; D016680:Genome, Bacterial; D008697:Methane / Q000378:metabolism*; D020629:Methylophilus / Q000235:genetics* / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D008697:Methane
keywords: First complete genome; Methane; Methanol; Methylophilus sp.; Tap-water