title: Retinal functional alterations in mice lacking intermediate filament proteins glial fibrillary acidic protein and vimentin.
abstract: Vimentin (Vim) and glial fibrillary acidic protein (GFAP) are important components of the intermediate filament (IF) (or nanofilament) system of astroglial cells. We conducted full-field electroretinogram (ERG) recordings and found that whereas photoreceptor responses (a-wave) were normal in uninjured GFAP(-/-)Vim(-/-) mice, b-wave amplitudes were increased. Moreover, we found that Kir (inward rectifier K(+)) channel protein expression was reduced in the retinas of GFAP(-/-)Vim(-/-) mice and that Kir-mediated current amplitudes were lower in Müller glial cells isolated from these mice. Studies have shown that the IF system, in addition, is involved in the retinal response to injury and that attenuated Müller cell reactivity and reduced photoreceptor cell loss are observed in IF-deficient mice after experimental retinal detachment. We investigated whether the lack of IF proteins would affect cell survival in a retinal ischemia-reperfusion model. We found that although cell loss was induced in both genotypes, the number of surviving cells in the inner retina was lower in IF-deficient mice. Our findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition.
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Kirsten A
authors.initials: KA
authors.lastname: Wunderlich
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tanimoto
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Grosche
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Eberhart
authors.initials: E
authors.lastname: Zrenner
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Milos
authors.initials: M
authors.lastname: Pekny
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Mathias W
authors.initials: MW
authors.lastname: Seeliger
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pannicke
authors.affiliation: *Department of Clinical Sciences, Division of Ophthalmology, and NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden; Graduate School of Cellular and Molecular Neuroscience, Center for Integrative Neuroscience (CIN), and Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany; Institute of Human Genetics, University of Regensburg, Regensburg, Germany; Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia; **Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia; and Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany maria_thereza.perez@med.lu.se.
authors.forename: Maria-Thereza
authors.initials: MT
authors.lastname: Perez
mesh_terms: D000818:Animals; D002470:Cell Survival; D004596:Electroretinography; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics*; D007511:Ischemia / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018408:Patch-Clamp Techniques; D012160:Retina / Q000503:physiopathology*; D012171:Retinal Vessels / Q000503:physiopathology; D014746:Vimentin / Q000235:genetics*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin
keywords: GFAP; Kir channels; Müller glia; electroretinogram; retinal ischemia