title: Crystallographic analysis of FAD-dependent glucose dehydrogenase.
abstract: An FAD-dependent glucose dehydrogenase (GDH) from Aspergillus terreus was purified and crystallized at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to a resolution of 1.6 Å from a single crystal at 100 K using a rotating-anode X-ray source. The crystal belonged to space group P21, with unit-cell parameters a = 56.56, b = 135.74, c = 74.13 Å, β = 90.37°. The asymmetric unit contained two molecules of GDH. The Matthews coefficient was calculated to be 2.2 Å(3) Da(-1) and the solvent content was estimated to be 44%.
authors.affiliation: Faculty of Education, Kagawa University, 1-1 Saiwai-cho, Takamatsu, Kagawa 760-8522, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Komori
authors.affiliation: Maruwa Foods and Biosciences Inc., 170-1 Tsutsui-cho, Yamatokoriyama, Nara 639-1123, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Inaka
authors.affiliation: Maruwa Foods and Biosciences Inc., 170-1 Tsutsui-cho, Yamatokoriyama, Nara 639-1123, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Furubayashi
authors.affiliation: Ikeda Tohka Industries Co. Ltd, 95-7 Minooki-cho, Fukuyama, Hiroshima 721-0956, Japan.
authors.forename: Michinari
authors.initials: M
authors.lastname: Honda
authors.affiliation: RIKEN SPring-8 Center, 1-1-1 Koto, Mikazuki-cho, Sayo-gun, Hyogo 679-5198, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000737:chemistry* / Q000201:enzymology; D001236:Aspergillus oryzae / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D005182:Flavin-Adenine Dinucleotide; D042843:Glucose 1-Dehydrogenase
keywords: FAD; glucose dehydrogenase; glucose sensor