title: Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect.
abstract: BACKGROUND
  Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines.
  
  
  METHODS
  Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as its methyl ester. The isolated methyl gamma linolenate was characterized by IR, (1)H NMR, (13)C NMR and mass spectral analysis and the data were consistent with the structure.
  
  
  RESULTS
  The percentage yield of isolated methyl gamma linolenate is found to be 71% w/w, which is a very good yield in comparison to other conventional methods. It was subjected to in-vitro cytotoxic screening on A-549 lung cancer cell lines using SRB assay and result was compared with standard rutin.
  
  
  CONCLUSIONS
  It may be concluded that the Flash chromatography system plays a major role in improving the yield for the isolation of methyl gamma linoleate from Spirulina platensis and the isolated molecule is a potent cytotoxic agent towards human lung carcinoma cell lines, however it may be further taken up for an extensive study.
authors.affiliation: Department of Pharmaceutical chemistry, JSS College of Pharmacy, JSS University, Mysore, India.
authors.forename: S
authors.initials: S
authors.lastname: Jubie
authors.affiliation: Department of Pharmacognosy and Phytopharmacy, J. S. S. College of Pharmacy, Rock lands, Ooty-643 001, Tamilnadu, India. spdhanabal@jsscpooty.org.
authors.forename: S P
authors.initials: SP
authors.lastname: Dhanabal
authors.affiliation: Research Associate, Department of Pharmacognosy and Phytopharmacy, JSS College of Pharmacy, Ooty-643001 JSS University, Mysore, India.
authors.forename: M V N L
authors.initials: MV
authors.lastname: Chaitanya
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002845:Chromatography; D006801:Humans; D053545:Spirulina / Q000737:chemistry*; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D017962:alpha-Linolenic Acid