title: Resveratrol inhibits oligomeric Aβ-induced microglial activation via NADPH oxidase.
abstract: Microglia‑mediated neuroinflammation is key in the pathogenesis of Alzheimer's disease (AD). Several studies have suggested that NADPH oxidase contributes to microglia‑mediated neuroinflammation. Resveratrol, which is a natural polyphenolic compound, exerts neuroprotective effects in AD due to its anti‑inflammatory properties. The present study aimed to investigate the effects of resveratrol on the activation of oligomeric amyloid β (oAβ)‑induced BV‑2 microglia, and to determine the role of NADPH oxidase in these effects. Microglial proliferation was measured by high‑content screening cell counting and using a bromodeoxyuridine incorporation assay. In addition, the levels of reactive oxygen species (ROS), nitric oxide (NO), tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β were assessed. The results of the present study demonstrated that resveratrol inhibited the proliferation of oAβ‑induced microglia and the production of pro‑inflammatory factors, including ROS, NO, TNF‑α and IL‑1β. Subsequent mechanistic investigations demonstrated that resveratrol inhibited the oAβ‑induced mRNA and protein expression levels of p47phox and gp91phox. These results suggested that NADPH oxidase may be a potential target for AD treatment, and resveratrol may be a valuable natural product possessing therapeutic potential against AD.
authors.affiliation: Department of Medical Chemistry, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Basic Traditional Chinese Medicine, School of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Yu
authors.affiliation: Department of Medical Chemistry, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Medical Chemistry, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Miao
authors.affiliation: Department of Medical Chemistry, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Xun-Xia
authors.initials: XX
authors.lastname: Zhao
authors.affiliation: Department of Medical Chemistry, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yuan-Jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Wan-Xia
authors.initials: WX
authors.lastname: Yao
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.affiliation: National Resource Center of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P.R. China.
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: Li
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D053583:Interleukin-1beta / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D000893:Anti-Inflammatory Agents; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D008562:Membrane Glycoproteins; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C488842:Cybb protein, mouse; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D000077185:Resveratrol