title: Impact of virus surface characteristics on removal mechanisms within membrane bioreactors.
abstract: In this study we investigated the removal of viruses with similar size and shape but with different external surface capsid proteins by a bench-scale membrane bioreactor (MBR). The goal was to determine which virus removal mechanisms (retention by clean backwashed membrane, retention by cake layer, attachment to biomass, and inactivation) were most impacted by differences in the virus surface properties. Seven bench-scale MBR experiments were performed using mixed liquor wastewater sludge that was seeded with three lab-cultured bacteriophages with icosahedral capsids of ∼30 nm diameter (MS2, phiX174, and fr). The operating conditions were designed to simulate those at a reference, full-scale MBR facility. The virus removal mechanism most affected by virus type was attachment to biomass (removals of 0.2 log for MS2, 1.2 log for phiX174, and 3 log for fr). These differences in removal could not be explained by electrostatic interactions, as the three viruses had similar net negative charge when suspended in MBR permeate. Removals by the clean backwashed membrane (less than 1 log) and cake layer (∼0.6 log) were similar for the three viruses. A comparison between the clean membrane removals seen at the bench-scale using a virgin membrane (∼1 log), and the full-scale using 10-year old membranes (∼2-3 logs) suggests that irreversible fouling, accumulated on the membrane over years of operation that cannot be removed by cleaning, also contributes towards virus removal. This study enhances the current mechanistic understanding of virus removal in MBRs and will contribute to more reliable treatment for water reuse applications.
authors.affiliation: ReNUWIt (Reinventing the Nation's Urban Water Infrastructure), Engineering Research Center, University of California, Berkeley, CA 94720, USA; Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720, USA.
authors.forename: Rabia M
authors.initials: RM
authors.lastname: Chaudhry
authors.affiliation: ReNUWIt (Reinventing the Nation's Urban Water Infrastructure), Engineering Research Center, University of California, Berkeley, CA 94720, USA; Department of Civil & Environmental Engineering, Colorado School of Mines, Golden, CO 80401, USA.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: Holloway
authors.affiliation: ReNUWIt (Reinventing the Nation's Urban Water Infrastructure), Engineering Research Center, University of California, Berkeley, CA 94720, USA; Department of Civil & Environmental Engineering, Colorado School of Mines, Golden, CO 80401, USA.
authors.forename: Tzahi Y
authors.initials: TY
authors.lastname: Cath
authors.affiliation: ReNUWIt (Reinventing the Nation's Urban Water Infrastructure), Engineering Research Center, University of California, Berkeley, CA 94720, USA; Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720, USA. Electronic address: karanelson@berkeley.edu.
authors.forename: Kara L
authors.initials: KL
authors.lastname: Nelson
mesh_terms: D001435:Bacteriophages / Q000302:isolation & purification; D019149:Bioreactors / Q000821:virology*; D008567:Membranes, Artificial; D014462:Ultrafiltration; D062065:Wastewater / Q000821:virology*; D018508:Water Purification / Q000379:methods
chemical_list: D008567:Membranes, Artificial; D062065:Wastewater
keywords: Bacteriophage fr; MS2; Mechanisms; Membrane bioreactor; PhiX174; Virus removal