title: Morphogenesis of respiratory syncytial virus in human primary nasal ciliated epithelial cells occurs at surface membrane microdomains that are distinct from cilia.
abstract: The distribution of cilia and the respiratory syncytial virus (RSV) nucleocapsid (N) protein, fusion (F) protein, attachment (G) protein, and M2-1 protein in human ciliated nasal epithelial cells was examined at between 1 and 5 days post-infection (dpi). All virus structural proteins were localized at cell surface projections that were distinct from cilia. The F protein was also trafficked into the cilia, and while its presence increased as the infection proceeded, the N protein was not detected in the cilia at any time of infection. The presence of the F protein in the cilia correlated with cellular changes in the cilia and reduced cilia function. At 5dpi extensive cilia loss and further reduced cilia function was noted. These data suggested that although RSV morphogenesis occurs at non-cilia locations on ciliated nasal epithelial cells, RSV infection induces changes in the cilia body that leads to extensive cilia loss.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Muhammad Raihan
authors.initials: MR
authors.lastname: Jumat
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119228, Singapore.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Laxmi Iyer
authors.initials: LI
authors.lastname: Ravi
authors.affiliation: Detection and Diagnostics Laboratory, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
authors.forename: Puisan
authors.initials: P
authors.lastname: Wong
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Tra Nguyen
authors.initials: TN
authors.lastname: Huong
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119228, Singapore.
authors.forename: Chunwei
authors.initials: C
authors.lastname: Li
authors.affiliation: Detection and Diagnostics Laboratory, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
authors.forename: Boon Huan
authors.initials: BH
authors.lastname: Tan
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119228, Singapore.
authors.forename: De Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore. Electronic address: rjsugrue@ntu.edu.sg.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Sugrue
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002923:Cilia / Q000737:chemistry / Q000821:virology; D004847:Epithelial Cells / Q000737:chemistry / Q000821:virology*; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000737:chemistry / Q000502:physiology*; D015678:Viral Structural Proteins / Q000032:analysis; D019065:Virus Assembly
chemical_list: D015678:Viral Structural Proteins
keywords: Ciliated epithelial cells; F-actin; Human nasal epithelial stem/progenitor cells; Respiratory syncytial virus; Virus morphogenesis