title: In vitro interaction of Stenotrophomonas maltophilia with human monocyte-derived dendritic cells.
abstract: Stenotrophomonas maltophilia is increasingly identified as an opportunistic pathogen in immunocompromised, cancer and cystic fibrosis (CF) patients. Knowledge on innate immune responses to S. maltophilia and its potential modulation is poor. The present work investigated the ability of 12 clinical S. maltophilia strains (five from CF patients, seven from non-CF patients) and one environmental strain to survive inside human monocyte-derived dendritic cells (DCs). The effects of the bacteria on maturation of and cytokine secretion by DCs were also measured. S. maltophilia strains presented a high degree of heterogeneity in internalization and intracellular replication efficiencies as well as in the ability of S. maltophilia to interfere with normal DCs maturation. By contrast, all S. maltophilia strains were able to activate DCs, as measured by increase in the expression of surface maturation markers and proinflammatory cytokines secretion.
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Roscetto
authors.affiliation: Laboratorio di Immunologia Cellulare e Molecolare, Istituto di Ricovero e Cura a Carattere Scientifico , San Raffaele Pisana, Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Vitiello
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Muoio
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
authors.forename: Amata A
authors.initials: AA
authors.lastname: Soriano
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
authors.forename: Vita D
authors.initials: VD
authors.lastname: Iula
authors.affiliation: DAI Medicina di Laboratorio, Azienda Ospedaliera Universitaria Federico II , Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Vollaro
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: De Gregorio
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Catania
keywords: DC maturation markers; cystic fibrosis; cytokines secretion; innate immune response; opportunistic pathogen