title: Integrated miRNA-mRNA analysis in the habenula nuclei of mice intravenously self-administering nicotine.
abstract: A considerable amount of evidence suggests that microRNAs (miRNAs) play crucial roles in the neuroadaptation of drug addiction. Habenula (Hb), one of the critical brain regions involved in reward and addiction, can be divided into two anatomically and transcriptionally distinct regions: medial habenula (MHb) and lateral habenula (LHb) nuclei. However, very few studies have compared the functional roles of these regions. Here, by using mirConnX integrator and KEGG pathway mapping, we simultaneously analysed the differential expression patterns of miRNAs and messenger RNA (mRNA) within MHb and LHb under nicotine addiction. Significantly altered miRNAs and mRNAs were found in the Hb of mice intravenously self-administering nicotine. Interestingly, some miRNAs were oppositely regulated between the MHb and the LHb, and their potential targets included various genes of cell signalling pathways related to the degeneration of fasciculus retroflexus (FR). This study provides an improved insight into the differential regulation of habenular transcripts in nicotine addiction, as well as the potential functions of miRNAs in several biological pathways involved in the nicotine addiction.
authors.affiliation: Center for Neuroscience, Brain Science Institute.
authors.forename: Sangjoon
authors.initials: S
authors.lastname: Lee
authors.affiliation: 1] Center for Neuroscience, Brain Science Institute [2] Research Animal Resource Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, Republic of Korea.
authors.forename: Jiwan
authors.initials: J
authors.lastname: Woo
authors.affiliation: Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Kim
authors.affiliation: 1] Center for Neuroscience, Brain Science Institute [2] Research Animal Resource Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, Republic of Korea [3] Neuroscience Program, Korea University of Science and Technology, 217 Gajungro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Heh-In
authors.initials: HI
authors.lastname: Im
mesh_terms: D000818:Animals; D001931:Brain Mapping; D005786:Gene Expression Regulation / Q000187:drug effects; D019262:Habenula / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009538:Nicotine / Q000008:administration & dosage / Q000009:adverse effects*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D014029:Tobacco Use Disorder / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D009538:Nicotine