title: [miRNA-101 inhibits the expression of the enhancer of zeste homolog 2 in androgen-independent prostate cancer LNCaP cell line].
abstract: OBJECTIVE
  To investigate the effect of miRNA-101 on the expression of the enhancer of zeste homolog 2 (EXH2) in human androgen-independent prostated cancer LNCaP cells.
  
  
  METHODS
  We divided LNCaP cells into a blank control, a negative control, and a miRNA-l01 transfection group, constructed the vector by transfecting synthetic miRNA-101 mimics into the LNCaP cells, and evaluated the efficiency of transfection by fluorescence microscopy. Then we determined the expression level of EZH2 mRNA by qRT-PCR in the three groups of cells and that of the EZH2 protein in the negative control and transfection groups by Western blot.
  
  
  RESULTS
  Green fluorescence signals were observed in over 70% of the LNCaP cells in the transfection group after 24 hours of transfection. At 72 hours, the expression of miRNA-101 was significantly upregulated in the transfected cells (P < 0.01), that of EZH2 mRNA was remarkably lower in the transfection group (0.01 ± 0.10) than in the blank control (0.95 ± 0.40) and negative control (0.86 ± 0.30) groups (both P < 0.01), and that of the EZH2 protein was increased in the negative control but decreased in the transfection group with the extension of culture time.
  
  
  CONCLUSIONS
  miRNA-101, with its inhibitory effect on the expression of EZH2 in LNCaP cells, is a potential biotherapeutic for prostate cancer.
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Liu
authors.forename: Qi-fa
authors.initials: QF
authors.lastname: Zhang
authors.forename: Chang-hai
authors.initials: CH
authors.lastname: Tian
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-zhou
authors.initials: XZ
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
mesh_terms: D000728:Androgens; D045744:Cell Line, Tumor; D000071221:Enhancer of Zeste Homolog 2 Protein; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D063151:Polycomb Repressive Complex 2 / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
chemical_list: D000728:Androgens; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2