title: Beneficial effects of vitamin E supplementation against the oxidative stress on Cylindrospermopsin-exposed tilapia (Oreochromis niloticus).
abstract: Cylindrospermopsin (CYN) is known to produce changes in some oxidative stress biomarkers in fish acutely and subchronically exposed to the toxin. The present study investigated the effects of vitamin E supplementation against the oxidative stress induced by pure CYN in tilapia (Oreochromis niloticus). Fish were pretreated with 700 mg vitamin E/kg fish body weight (bw)/day for 7 days by oral route, and on day seven, they received a single oral dose of 400 μg pure CYN/kg fish bw, and were killed after 24 h. The biomarkers evaluated included lipid peroxidation (LPO), protein and DNA oxidation, glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and γ-glutamyl-cysteine synthetase (γ-GCS) activities, and ratio of reduced glutathione-oxidized glutathione (GSH/GSSG). This is the first study showing that vitamin E supplementation is effective at reducing the toxicity induced by CYN, recovering the biomarkers assayed to basal levels. Therefore, vitamin E can be considered a useful chemoprotectant that reduces hepatic and renal oxidative stress and can be used in the prophylaxis and treatment of CYN-related intoxication in fish.
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Spain. Electronic address: rguzman1@us.es.
authors.forename: Remedios
authors.initials: R
authors.lastname: Guzmán-Guillén
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Prieto Ortega
authors.affiliation: Stomatology Department, School of Dentistry, University of Sevilla, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Martín-Caméan
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Cameán
mesh_terms: D000470:Alkaloids; D000818:Animals; D001427:Bacterial Toxins; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D000087522:Cyanobacteria Toxins; D004305:Dose-Response Relationship, Drug; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000378:metabolism; D017210:Tilapia; D014498:Uracil / Q000031:analogs & derivatives* / Q000633:toxicity; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D015415:Biomarkers; D000087522:Cyanobacteria Toxins; D014810:Vitamin E; C089595:cylindrospermopsin; D014498:Uracil; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005721:Glutamate-Cysteine Ligase
keywords: Cylindrospermopsin; Fish; Oreochromis niloticus; Oxidative stress; Vitamin E