title: Assessing the Impact of Mechanical Damage on Full-Thickness Porcine and Human Skin Using an In Vitro Approach.
abstract: For most xenobiotics, the rates of percutaneous absorption are limited by diffusion through the horny layer of skin. However, percutaneous absorption of chemicals may seriously increase when the skin is damaged. The aim of this work was to develop an in vitro representative model of mechanically damaged skins. The epidermal barrier was examined following exposure to a razor, a rotating brush, and a microneedle system in comparison to tape-stripping which acted as a reference. Excised full-thickness skins were mounted on a diffusion chamber in order to evaluate the effect of injuries and to mimic physiological conditions. The transepidermal water loss (TEWL) was greatly increased when the barrier function was compromised. Measurements were made for all the damaged biopsies and observed histologically by microscopy. On human and porcine skins, the tape-stripping application (0 to 40 times) showed a proportional increase in TEWL which highlights the destruction of the stratum corneum. Similar results were obtained for all cosmetic instruments. This is reflected in our study by the nonsignificant difference of the mean TEWL scores between 30 strips and mechanical damage. For a specific appreciation, damaged skins were then selected to qualitatively evaluate the absorption of a chlorogenic acid solution using fluorescence microscopy.
authors.affiliation: Faculty of Pharmacy, IBMM-UMR 5247, University of Montpellier, France.
authors.forename: Hinda
authors.initials: H
authors.lastname: Dabboue
authors.affiliation: Tissue Bank and CCBHM, Saint Eloi Hospital, Montpellier, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Builles
authors.affiliation: Pathology Department, University Hospital of Poitiers, France.
authors.forename: Éric
authors.initials: É
authors.lastname: Frouin
authors.affiliation: Faculty of Pharmacy, IBMM-UMR 5247, University of Montpellier, France.
authors.forename: Dan
authors.initials: D
authors.lastname: Scott
authors.affiliation: Service of Anatomy and Cytopathology, Gui de Chauliac Hospital, CHU, University of Montpellier, France.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Ramos
authors.affiliation: Faculty of Pharmacy, IBMM-UMR 5247, University of Montpellier, France.
authors.forename: Gilberte
authors.initials: G
authors.lastname: Marti-Mestres
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism*; D006801:Humans; D010812:Physical Stimulation / Q000009:adverse effects*; D012867:Skin / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology*; D017695:Soft Tissue Injuries / Q000473:pathology / Q000503:physiopathology; D013045:Species Specificity; D013314:Stress, Mechanical; D013552:Swine; D014870:Water Loss, Insensible