title: Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.
abstract: In this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2. We utilized the heat-resistant characteristics of SSP2 to purify the protein and maintain its biological activity. In addition, using flow cytometry and the MTT assay, we found that SSP2 had anti-apoptotic effects on BmN cells, and that SSP2 could also inhibit cell apoptosis induced by chemical factors. These results suggest that SSP2 has a cell-protective function, and provides a basis for future work on the function of storage proteins in silkworm.
authors.affiliation: Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China, cjqgqj@126.com.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Chen
authors.forename: Tejun
authors.initials: T
authors.lastname: Shu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Man
authors.initials: M
authors.lastname: Ye
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Nie
authors.forename: Qijing
authors.initials: Q
authors.lastname: Gai
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Yaozhou
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D012831:Bombyx / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006358:Hot Temperature; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D055550:Protein Stability; D011679:Pupa / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins