title: Neuroprotective Effects of Castanea sativa Mill. Bark Extract in Human Neuroblastoma Cells Subjected to Oxidative Stress.
abstract: One of the major features of neurodegenerative disease is the selective vulnerability of different neuronal populations that are affected in a progressive and often stereotyped manner. Despite the susceptible neuronal population varies between diseases, oxidative stress is implicated as the major pathogenic process in all of them. Natural Extract of Castanea sativa Mill. bark (ENC), recently characterized in its phenolic composition, acts as antioxidant and cardioprotective agent. Its neuroprotettive properties, however, have never been investigated. The aim of this study was to assess neuroprotection of ENC in in vitro models of oxidative-stress-mediate injury. Human neuroblastoma SH-SY5Y cells treated with glutamate (50 mM for 24 h) or hydrogen peroxide (25 μM for 1 h followed by 24 with medium) were used. The results showed that the addition of ENC (1-50 μg/ml) to cell medium before the neuronal damage provided neuroprotection in both experimental models used, while its addition after the injury was ineffective. In conclusion, the present results suggest that ENC could be a valuable support as dietary supplement, combining beneficial preventive neuroprotettive effects with a high antioxidant activity.
authors.affiliation: Dipartimento di Scienze della Vita, Università di Siena Via Aldo Moro 2, Siena-53100, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Brizi
authors.affiliation: Dipartimento di Scienze della Vita, Università di Siena Via Aldo Moro 2, Siena-53100, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Santulli
authors.affiliation: Dipartimento di Farmacia e Biotecnologie, Università di Bologna Via Belmeloro 6, Bologna-40126, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Micucci
authors.affiliation: Dipartimento di Farmacia e Biotecnologie, Università di Bologna Via Belmeloro 6, Bologna-40126, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Budriesi
authors.affiliation: Dipartimento di Farmacia e Biotecnologie, Università di Bologna Via Belmeloro 6, Bologna-40126, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Chiarini
authors.affiliation: Dipartimento di Medicina Molecolare e dello Sviluppo, Università di Siena Via Aldo Moro 2, Siena-53100, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Aldinucci
authors.affiliation: Dipartimento di Scienze della Vita, Università di Siena Via Aldo Moro 2, Siena-53100, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Frosini
mesh_terms: D045744:Cell Line, Tumor; D048430:Cell Shape; D002470:Cell Survival; D004353:Drug Evaluation, Preclinical; D029962:Fagaceae / Q000737:chemistry*; D018698:Glutamic Acid / Q000633:toxicity; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009447:Neuroblastoma; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; D018698:Glutamic Acid; D006861:Hydrogen Peroxide
keywords: Castanea sativa Mill; EXCITOTOXICITY; NEURODEGENERATIVE DISEASES; NEUROPROTECTION; OXIDATIVE STRESS