title: Combination of two-dimensional gel electrophoresis and a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue allows the identification of intracellular cisplatin-protein adducts.
abstract: Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin-protein adducts was developed using a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), 2DE, and ESI-MS/MS. We identified several CFDA-cisplatin-protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA-cisplatin-protein adducts, which may help to understand the resistance mechanism of cisplatin.
authors.affiliation: Biocenter MS Platform, Department of Biology, University of Cologne, Cologne, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Kotz
authors.affiliation: Department of Clinical Pharmacy, Institute of Pharmacy, University of Bonn, Bonn, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Kullmann
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Crone
authors.affiliation: Department of Clinical Pharmacy, Institute of Pharmacy, University of Bonn, Bonn, Germany.
authors.forename: Ganna V
authors.initials: GV
authors.lastname: Kalayda
authors.affiliation: Department of Clinical Pharmacy, Institute of Pharmacy, University of Bonn, Bonn, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Jaehde
authors.affiliation: Biocenter MS Platform, Department of Biology, University of Cologne, Cologne, Germany.|IUF-Leibniz Research Institute for Environmental Medicine, IUF, Düsseldorf, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Metzger
keywords: Carboxyfluorescein-diacetate-labeled cisplatin analogue; Cisplatin; Cisplatin-protein adducts; Protein marker grid; Two-dimensional gel electrophoresis