title: Thermal stress resistance and aging effects of Panax notoginseng polysaccharides on Caenorhabditis elegans.
abstract: Panax notoginseng attract public attention due to their potential biomedical properties and corresponding health benefits. The present study investigated the anti-aging and thermal stress resistance effects of polysaccharides from P. notoginseng on Caenorhabditis elegans. Results showed polysaccharides had little scavenging ability of reactive oxygen species (ROS) in vitro, but significantly extended lifespan of C. elegans, especially the main root polysaccharide (MRP) which prolongs the mean lifespan of wild type worms by 21%. Further study demonstrated that the heat stress resistance effect of polysaccharides on C. elegans might be attributed to the elevation of antioxidant enzyme activities (both superoxide dismutase (SOD) and catalase (CAT)) and the reduction lipid peroxidation of malondialdehyde (MDA) level. Taken together, the results provided a scientific basis for the further exploitation of the mechanism of longer lifespan controlled by P. notoginseng polysaccharides on C. elegans. The P. notoginseng polysaccharides might be considered as a potential source to delay aging.
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Shiling
authors.initials: S
authors.lastname: Feng
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg 69120, Germany.
authors.forename: Haoran
authors.initials: H
authors.lastname: Cheng
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Shian
authors.initials: S
authors.lastname: Shen
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China. Electronic address: dcb@sicau.edu.cn.
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
mesh_terms: D000220:Adaptation, Biological; D000375:Aging / Q000378:metabolism; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006358:Hot Temperature; D008136:Longevity; D008315:Malondialdehyde; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D053723:Panax notoginseng / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature
chemical_list: D016166:Free Radical Scavengers; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: Antioxidant enzyme activities; Caenorhabditis elegans; Panax notoginseng; Polysaccharides; Thermal stress