title: Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription.
abstract: For therapeutic applications of siRNA, there are technical challenges with respect to targeted and systemic delivery. We here report a new siRNA carrier, RNAtr NPs, in a way that multiple tandem copies of RNA hairpins as a result of rolling circle transcription (RCT) can be readily adapted in tumour-targeted and systemic siRNA delivery. RNAtr NPs provide a means of condensing large amounts of multimeric RNA transcripts into the compact nanoparticles, especially without the aid of polycationic agents, and thus reduce the risk of immunogenicity and cytotoxicity by avoiding the use of synthetic polycationic reagents. This strategy allows the design of a platform technology for systemic delivery of siRNA to tumour sites, because RCT reaction, which enzymatically generates RNA polymers in multiple copy numbers at low cost, can lead to directly accessible routes to targeted and systemic delivery. Therefore, RNAtr NPs suggest great potentials as the siRNA therapeutics for cancer treatment.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea.
authors.forename: Mihue
authors.initials: M
authors.lastname: Jang
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea.
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Pungnap-2 dong, Songpa-Gu, Seoul 136-736, South Korea.
authors.forename: Hae Yun
authors.initials: HY
authors.lastname: Nam
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea.
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-Gu, Seoul 136-791, South Korea.
authors.forename: Hyung Jun
authors.initials: HJ
authors.lastname: Ahn
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D016503:Drug Delivery Systems; D005260:Female; D005492:Folic Acid / Q000737:chemistry; D020868:Gene Silencing; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D053758:Nanoparticles; D012313:RNA / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D014158:Transcription, Genetic; D023041:Xenograft Model Antitumor Assays
chemical_list: D034741:RNA, Small Interfering; D012313:RNA; D004247:DNA; D005492:Folic Acid; D002784:Cholesterol