title: Binding of the 5'-Triphosphate End of mRNA to the γ-Subunit of Translation Initiation Factor 2 of the Crenarchaeon Sulfolobus solfataricus.
abstract: The heterotrimeric archaeal IF2 orthologue of eukaryotic translation initiation factor 2 consists of the α-subunit, β-subunit and γ-subunit. Previous studies showed that the γ-subunit of aIF2, besides its central role in Met-tRNAi binding, has an additional function: it binds to the 5'-triphosphorylated end of mRNA and protects its 5'-part from degradation. Competition studies with nucleotides and mRNA, as well as structural and kinetic analyses of aIF2γ mutants, strongly implicate the canonical GTP/GDP-binding pocket in binding to the 5'-triphosphate end of mRNAs. The biological implication of these findings is being discussed.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Valentina
authors.initials: V
authors.lastname: Arkhipova
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Stolboushkina
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Olesya
authors.initials: O
authors.lastname: Kravchenko
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Kljashtorny
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Azat
authors.initials: A
authors.lastname: Gabdulkhakov
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Maria
authors.initials: M
authors.lastname: Garber
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Nikonov
authors.affiliation: Max F. Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Vienna Biocenter, Dr. Bohrgasse 9/4, A-1030 Vienna, Austria.
authors.forename: Birgit
authors.initials: B
authors.lastname: Märtens
authors.affiliation: Max F. Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Vienna Biocenter, Dr. Bohrgasse 9/4, A-1030 Vienna, Austria.
authors.forename: Udo
authors.initials: U
authors.lastname: Bläsi
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation. Electronic address: alik@vega.protres.ru.
authors.forename: Oleg
authors.initials: O
authors.lastname: Nikonov
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D006160:Guanosine Triphosphate / Q000378:metabolism; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits; D019642:RNA, Archaeal / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D048229:Sulfolobus solfataricus / Q000737:chemistry / Q000378:metabolism*
chemical_list: C118735:IF2 protein, archaeal; D010448:Peptide Initiation Factors; D021122:Protein Subunits; D019642:RNA, Archaeal; D012333:RNA, Messenger; D006160:Guanosine Triphosphate
keywords: GTP; a/eIF2γ; archaea; crystal structure; mRNA