title: Strong Manual Acupuncture Manipulation Could Better Inhibit Spike Frequency of the Dorsal Horn Neurons in Rats with Acute Visceral Nociception.
abstract: Afferent impulses from visceral nociception can be regulated by acupuncture at spinal cord level; however, the effects of different manual acupuncture (MA) manipulations on the afferent impulses are still unknown. Here, we analyzed the spike frequency of excitatory gastric-related wide dynamic range (WDR) neurons in spinal dorsal horn (SDH) following acute gastric distension (GD) in rats and compared their responses to MA manipulations with four different frequencies (0.5, 1, 2, and 3 Hz) at Zusanli (ST36). Results indicated that the spike frequency was increased by acute GD stimulation. Under acute GD circumstances, the spike frequency was further activated by weak MA stimulation (0.5 and 1 Hz), while being significantly inhibited by strong MA stimulation (2 and 3 Hz). After 10 minutes of the strong MA stimulation, same intensity of acute GD caused less spike frequency. Our previous researches had demonstrated that different MA manipulations could increase spike frequency in an intensity-dependent manner in normal rats; these findings suggest that acupuncture may have different modulatory effects depending on the state of the stomach. Since neuronal spike frequency was related to the level of nociception, the results suggest that strong MA manipulation may have better effect on acute visceral nociception.
authors.affiliation: Acupuncture Department, Zhejiang Provincial Hospital of TCM, No. 54, Youdian Road, Hangzhou 310006, China ; Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Shouhai
authors.initials: S
authors.lastname: Hong
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China ; Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of TCM, Wuhu First People's Hospital, No. 19, Jiehebeit Road, Jinghu District, Wuhu 241000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China ; Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xi
authors.affiliation: Department of Rehabilitation and Health Care, Hunan Traditional Chinese Medical College, Zhuzhou 412012, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China ; Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Qing
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China ; Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China ; Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo