title: Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation.
abstract: Cyclin-dependent kinases (Cdks) belong to a family of key regulators of cell division cycle and transcription. Their activity is mainly regulated by association with regulatory subunits named cyclins but their activities are also regulated by phosphorylation, acetylation, and the association with specific inhibitory proteins (CKIs). The activity of different Cdks is deregulated in many different type of tumors, and thus, Cdks are considered targets for antitumoral therapy. For large screenings of inhibitors the use of purified recombinant Cdks and cyclins is recommended. We report here the current methods to determine their in vitro activity for large screenings of inhibitors.
authors.affiliation: Department of Cell Biology, Immunology and Neurosciences, University of Barcelona, Barcelona, Spain.
authors.forename: Edurne
authors.initials: E
authors.lastname: Gallastegui
authors.forename: Oriol
authors.initials: O
authors.lastname: Bachs
mesh_terms: D001419:Bacteria / Q000378:metabolism; D016203:CDC2 Protein Kinase / Q000737:chemistry / Q000302:isolation & purification; D002453:Cell Cycle; D051357:Cyclin-Dependent Kinase 2 / Q000737:chemistry / Q000302:isolation & purification; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000302:isolation & purification*; D016213:Cyclins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D009369:Neoplasms / Q000378:metabolism; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D016213:Cyclins; D011994:Recombinant Proteins; D016203:CDC2 Protein Kinase; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases