title: Chemical constituents and antioxidant activity of the essential oil from leaves of Annona vepretorum Mart. (Annonaceae).
abstract: BACKGROUND
  Annona vepretorum (AV) is a native tree from Caatinga biome (semiarid region of Brazil) popularly known as "araticum" and "pinha da Caatinga."
  
  
  OBJECTIVE
  This study was carried out to evaluate the chemical constituents and antioxidant activity (AA) of the essential oil from the leaves from AV (EO-Av) collected in Petrolina, Pernambuco, Brazil.
  
  
  METHODS
  Fresh leaves of AV were cut into pieces, and subjected to distillation for 2 h in a clevenger-type apparatus. Gas chromatograph (GC) analyses were performed using a mass spectrometry/flame ionization detector. The identification of the constituents was assigned on the basis of comparison of their relative retention indices. The antioxidant ability of the EO was investigated through two in vitro models such as radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method and β-carotene-linoleate-model system. The positive controls (ascorbic acid, butylated hydroxyanisole and butylated hydroxytoluene) were those using the standard solutions. Assays were carried out in triplicate.
  
  
  RESULTS
  The oil showed a total of 21 components, and 17 were identified, representing 93.9% of the crude EO. Spathulenol (43.7%), limonene (20.5%), caryophyllene oxide (8.1%) and α-pinene (5.5%) were found to be the major individual constituents. Spathulenol and caryophyllene oxide could be considered chemotaxonomic markers of these genera. The EO demonstrated weak AA.
authors.affiliation: Center for Studies and Research of Medicinal Plants, Federal University of Vale do S o Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
authors.forename: Camila de Souza
authors.initials: Cde S
authors.lastname: Araújo
authors.affiliation: Center for Studies and Research of Medicinal Plants, Federal University of Vale do S o Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Oliveira
authors.affiliation: Department of Chemistry, Federal University of Sergipe, Campus Prof. Alo sio de Campos, 49.100-000, S o Crist v o, Sergipe, Brazil.
authors.forename: Rafaely Nascimento
authors.initials: RN
authors.lastname: Lima
authors.affiliation: Department of Chemistry, Federal University of Sergipe, Campus Prof. Alo sio de Campos, 49.100-000, S o Crist v o, Sergipe, Brazil.
authors.forename: Péricles Barreto
authors.initials: PB
authors.lastname: Alves
authors.affiliation: Center for Studies and Research of Medicinal Plants, Federal University of Vale do S o Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
authors.forename: Tâmara Coimbra
authors.initials: TC
authors.lastname: Diniz
authors.affiliation: Center for Studies and Research of Medicinal Plants, Federal University of Vale do S o Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
authors.forename: Jackson Roberto Guedes
authors.initials: JR
authors.lastname: da Silva Almeida
keywords: Annona vepretorum; annonaceae; antioxidant activity; essential oil