title: Use of new endophytic fungi as pretreatment to enhance enzymatic saccharification of Eucalyptus globulus.
abstract: New endophytic fungi are assessed for the first time as pretreatment to enhance saccharification of Eucalyptus globulus wood. The fungi are all laccase-producing ascomycetes and were isolated from eucalyptus trees in Spain. After five endophytes had been assayed alone or in combination with white-rot fungus Trametes sp. I-62, three were pre-selected. To improve sugar production, an autohydrolysis pretreatment was performed before or after fungal treatment. Pretreatment increased sugar production 2.7 times compared to non-pretreated wood. When fungal and autohydrolysis pretreatments were combined, a synergistic increase in saccharification was observed in all cases. Endophytic fungi Ulocladium sp. and Hormonema sp. produced greater enhancements in saccharification than Trametes sp. I-62 (increase in sugar yields of 8.5, 8.0 and 6.0 times, respectively), demonstrating the high potential of these new endophytic fungi for saccharification enhancement.
authors.affiliation: INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain. Electronic address: martin.raquel@inia.es.
authors.forename: Raquel
authors.initials: R
authors.lastname: Martín-Sampedro
authors.affiliation: INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: Úrsula
authors.initials: Ú
authors.lastname: Fillat
authors.affiliation: INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Ibarra
authors.affiliation: INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: María E
authors.initials: ME
authors.lastname: Eugenio
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D001709:Biotechnology / Q000379:methods; D050260:Carbohydrate Metabolism; D060026:Endophytes / Q000502:physiology*; D005052:Eucalyptus / Q000378:metabolism* / Q000382:microbiology*; D006868:Hydrolysis; D042845:Laccase / Q000378:metabolism; D013030:Spain; D055454:Trametes / Q000502:physiology; D014934:Wood / Q000378:metabolism / Q000382:microbiology*
chemical_list: D042845:Laccase
keywords: Ascomycetes; Dothideomycetes; Endophytes; Enzymatic saccharification; Pretreatment