title: Poly-L-Arginine Acts Synergistically with LPS to Promote the Release of IL-6 and IL-8 via p38/ERK Signaling Pathways in NCI-H292 Cells.
abstract: Major basic protein (MBP) derived from activated eosinophil can exacerbate atopic asthma induced by lipopolysaccharide (LPS). The pharmacological function of MBP can be mimicked by poly-L-arginine (PLA), however, the potential signaling mechanisms of LPS-PLA-induced release of the inflammatory cytokines interleukin (IL)-6 and IL-8 remain unclear. In the present study, airway epithelia NCI-H292 cell lines were treated with LPS and/or PLA. We found that the expression levels of IL-6 and IL-8 induced by LPS-PLA were increased significantly compared with that in untreated cells. Meanwhile, the phosphorylation of p38 MAPK and ERK1/2 was also up-regulated dramatically by LPS-PLA, but this increase could be blocked by specific inhibitor. Importantly, blocking the phosphorylation of p38 MAPK and ERK1/2 reduced the expression levels of IL-6 and IL-8 as well. Collectively, LPS-PLA-induced release of IL-6 and IL-8 from NCI-H292 cells may be due to the synergistic activation of p38 MAPK and ERK1/2 signaling transduction pathways.
authors.affiliation: Department of Pulmonology, The Geriatric Institute of Anhui, The First Affiliated Hospital of Anhui Medical University, Number 218, Jixi Road, Hefei, Anhui, 230022, People's Republic of China. smallcloud2@126.com.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Fan
authors.affiliation: Department of Pulmonology, The Geriatric Institute of Anhui, The First Affiliated Hospital of Anhui Medical University, Number 218, Jixi Road, Hefei, Anhui, 230022, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Pulmonology, The Geriatric Institute of Anhui, The First Affiliated Hospital of Anhui Medical University, Number 218, Jixi Road, Hefei, Anhui, 230022, People's Republic of China.
authors.forename: Zhao-Shuang
authors.initials: ZS
authors.lastname: Lu
authors.affiliation: Department of Pulmonology, The Geriatric Institute of Anhui, The First Affiliated Hospital of Anhui Medical University, Number 218, Jixi Road, Hefei, Anhui, 230022, People's Republic of China.
authors.forename: Zi-Feng
authors.initials: ZF
authors.lastname: Jiang
authors.affiliation: Department of Biochemistry and Molecular Biology, Anhui Medical University, Number 81, Meishan Road, Hefei, Anhui, 230022, People's Republic of China.
authors.forename: Sheng-Quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D001249:Asthma / Q000473:pathology*; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects; D047093:Eosinophil Major Basic Protein / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D020545:Respiratory Mucosa / Q000166:cytology / Q000502:physiology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C508606:CXCL8 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010455:Peptides; C015462:polyarginine; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D047093:Eosinophil Major Basic Protein
keywords: airway epithelial cell; asthma; interleukin-6; interleukin-8; poly-L-arginine