title: Cellular Response to Linear and Branched Poly(acrylic acid).
abstract: Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.
authors.affiliation: University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Whitty
authors.affiliation: University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.
authors.forename: Alison R
authors.initials: AR
authors.lastname: Maniego
authors.affiliation: University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.
authors.forename: Sharon A
authors.initials: SA
authors.lastname: Bentwitch
authors.affiliation: CNRS, Institut de Chimie Radicalaire, Aix-Marseille Université, UMR 7273, 13397, Marseille, France.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Guillaneuf
authors.affiliation: University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Jones
authors.affiliation: University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.|University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.|University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.
authors.forename: Marianne
authors.initials: M
authors.lastname: Gaborieau
authors.affiliation: University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.|University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.
authors.forename: Patrice
authors.initials: P
authors.lastname: Castignolles
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D051379:Mice; D032448:Myoblasts, Skeletal / Q000378:metabolism*
chemical_list: D000180:Acrylic Resins; D001798:Blood Proteins; C006903:carbopol 940
keywords: MTT assay; capillary electrophoresis; cell; cytotoxicity; poly(acrylic acid)/poly(sodium acrylate)