title: In vitro study of cyclosporine A 0.05 % on primary and recurrent pterygium fibroblasts.
abstract: To compare the cyclosporine 0.05 % exposure effect on fibroblasts from primary and recurrent pterygium. Primary culture of fibroblasts from primary and recurrent pterygium was performed until the third passage, which was exposed to cyclosporine 0.05 % in a group and the other remaining unexposed (control group), in triplicates. After 3, 6, 12, and 17 days of exposure the viable cell counting was performed by hemocytometer. The results were statistically analyzed using the technique of analysis of non-parametric variance model for repeated measures with three factors. There was a significant reduction in both fibroblast proliferation, in primary as in the recurrent pterygium cultures exposed to cyclosporine when compared not exposed cultures, with statistical significance (P < 0.05). Comparing primary and recurrent pterygium that received the drug, there was no significant difference in cell proliferation in relation to primary or recurrent pterygium. Cyclosporine 0.05 % is effective in inhibiting fibroblast proliferation in culture, both in primary and as in recurrent pterygium.
authors.affiliation: Post-Doctoral of the General Basis of Surgery Post Graduation Program of Botucatu Medical School, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil. magdahata@yahoo.com.|Department of Ophthalmology, Botucatu Medical School, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil. magdahata@yahoo.com.
authors.forename: Magda Massae Hata
authors.initials: MM
authors.lastname: Viveiros
authors.affiliation: Department of Ophthalmology, Botucatu Medical School, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil.
authors.forename: Fabiano Yutaka
authors.initials: FY
authors.lastname: Kakizaki
authors.affiliation: Department of Ophthalmology, Botucatu Medical School, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil.
authors.forename: Laura Almeida
authors.initials: LA
authors.lastname: Hércules
authors.affiliation: Department of Biostatistic, Biosciences Institute, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil.
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Padovani
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil.
authors.forename: João Manuel Grisi
authors.initials: JM
authors.lastname: Candeias
authors.affiliation: Department of Ophthalmology, Botucatu Medical School, Universidade Estadual Paulista - UNESP de Botucatu, São Paulo, Brazil.
authors.forename: Silvana Artioli
authors.initials: SA
authors.lastname: Schellini
mesh_terms: D000704:Analysis of Variance; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D011446:Prospective Studies; D011625:Pterygium / Q000188:drug therapy*
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine
keywords: Cyclosporine; Fibroblasts; In vitro; Pterygium