title: Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits.
abstract: A water-soluble polysaccharide namely RCP-II from raspberry fruits was obtained by complex enzyme method followed by successive purification using macroporous resin D4020 and Sephadex G-100 columns. RCP-II was an acidic heteropolysaccharide and the characteristic structure of polysaccharide was determined. The carbohydrate of RCP-II was composed with galacturonic acid, rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.00:0.55:1.19:0.52:0.44:1.90 and the average molecular weight was estimated to be 4013 Da, based on dextran standards. RCP-II presented high scavenging activity toward DPPH•, HO•, O2(•-) in a concentration-dependent manner. The determination of the inhibitory activity on protein glycation showed that in 14 days of incubation the inhibitory ability of RCP-II was more effective on the development of non-enzymatic glycation reaction at early phase than that at the following two phases.
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: wanglibo99166@163.com.
authors.forename: Libo
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Teng
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D002417:Cattle; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D006031:Glycosylation / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D065856:Rubus / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides; D012710:Serum Albumin, Bovine
keywords: Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Carbazole (PubChem CID: 6854); DPPH (PubChem CID: 2735032); Girard-T (PubChem CID: 67156); Non-enzymatic glycation inhibition activity; Polysaccharide; Purification; Rubus idaeus L.; Trifluoroacetic acid (PubChem CID: 6422); d-Arabinose (PubChem CID: 66308); d-Galacturonic acid (PubChem CID: 84740); d-Glucose (PubChem CID: 5793)