title: Cytoprotective effects of grape seed extract on human gingival fibroblasts in relation to its antioxidant potential.
abstract: Cytoprotective effects of short-term treatment with grape seed extract (GSE) upon human gingival fibroblasts (hGFs) were evaluated in relation to its antioxidant properties and compared with those of a water-soluble analog of vitamin E: trolox (Tx). GSE and Tx showed comparable antioxidant potential in vitro against di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH; a stable radical), hydroxyl radical (•OH), singlet oxygen (1O2), and hydrogen peroxide (H2O2). Pretreatment or concomitant treatment with GSE for 1 min protected hGFs from oxidative stressors, including H2O2, acid-electrolyzed water (AEW), and 1O2, and attenuated the intracellular formation of reactive oxygen species induced by H2O2 and AEW. Tx also reduced the H2O2- and AEW-induced intracellular formation of reactive oxygen species, but showed no cytoprotective effects on hGFs exposed to H2O2, AEW, or 1O2. These results suggest that the cytoprotective effects of GSE are likely exerted independently of its antioxidant potential.
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Katsuda
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Niwano
authors.affiliation: Kiasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Nakashima
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Mokudai
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Oizumi
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Kanno
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kanetaka
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egusa
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000187:drug effects / Q000378:metabolism*; D056604:Grape Seed Extract / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D056604:Grape Seed Extract; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide