title: High-Level Butanol Production from Cassava Starch by a Newly Isolated Clostridium acetobutylicum.
abstract: A new Clostridium acetobutylicum strain, exhibiting the ability to resist butanol stress and produce butanol, was identified and named GX01. Strain GX01 can use a wide variety of carbohydrates, especially cassava starch, to produce butanol. After the optimization of culture conditions, C. acetobutylicum GX01 could produce 27.3 g/L solvent, including 17.1 g/L butanol, 7.9 g/L acetone, and 2.3 g/L ethanol, from 100 g/L cassava flour and 3 g/L soybean meal. Furthermore, when its acetone-butanol-ethanol (ABE) fermentation was performed in 10- and 30-L bioreactors, the production of total solvent and butanol reached 29.2 and 18.3 g/L, respectively, and 28.8 and 18.8 g/L, respectively. Thus, the high level and stability of butanol production make strain GX01 a promising candidate for ABE fermentation using the low-cost cassava starch.
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China.
authors.forename: Shubo
authors.initials: S
authors.lastname: Li
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, 530004, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Chemistry and Life Science, Hechi University, Yizhou, Guangxi, 546300, China.
authors.forename: Fuzhi
authors.initials: F
authors.lastname: Lu
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, 530004, China.|Guangxi Bio-Based Chemicals Engineering Research Center, Guiping, Guangxi, 537200, China.
authors.forename: Jiajian
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, 530004, China. pangzw@gxu.edu.cn.|Guangxi Bio-Based Chemicals Engineering Research Center, Guiping, Guangxi, 537200, China. pangzw@gxu.edu.cn.|Guangxi Jin Yuan Biochemical Industrial Co., Ltd, Guiping, Guangxi, 537200, China. pangzw@gxu.edu.cn.
authors.forename: Zongwen
authors.initials: Z
authors.lastname: Pang
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D002365:Manihot / Q000737:chemistry*; D009584:Nitrogen / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013213:Starch / Q000378:metabolism*
chemical_list: D000440:Butanols; D004269:DNA, Bacterial; D013213:Starch; D009584:Nitrogen
keywords: ABE fermentation; Buatanol; Cassava starch; Clostridium acetobutylicum