title: MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB.
abstract: In gastric carcinoma, the nuclear factor‑κB (NF‑κB) signaling pathway is highly active, and the constitutive activation of NF‑κB prompts malignant cell proliferation. MicroRNAs are considered to be important mediators in the regulation of the NF‑κB signaling pathway. The present study predominantly focussed on the effects of microRNA (miR)‑19a on NF‑κB activation. Reverse transcription‑quantitative polymerase chain reaction was used to quantify the relative levels of miR‑19a in gastric carcinoma cells. MTT assays were used to determine the effect of miR‑19a on cellular proliferation. To detect the activation of NF‑κB, western blotting was performed to measure the protein levels of NF‑κB and the products of its downstream target genes. To define the target genes, luciferase reporter assays were used. miR‑19a was found to be markedly upregulated in gastric carcinoma cells. The overexpression of miR‑19a resulted in proliferation and enhanced migratory capabilities of the MGC‑803 gastric carcinoma cell line. The results of the western blot analysis demonstrated that the protein levels of p65 increased when the MGC‑803 cells were transfected with miR‑19a mimics. In addition, the downstream target genes of miR‑19a, including intercellular adhesion molecule, vascular cell adhesion molecule and monocyte chemoattractant protein‑1, were upregulated. The results of the luciferase assay indicated that IκB‑α was the target gene of miR‑19a. Therefore, the results of the present study suggested that miR‑19a enhances malignant gastric cell proliferation by constitutively activating the NF‑κB signaling pathway.
authors.affiliation: Department of Tumor Research and Therapy Center, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Oncology, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Oncology, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Oncology, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
authors.forename: Anxiang
authors.initials: A
authors.lastname: Hu
authors.affiliation: Department of Oncology, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Chu
authors.affiliation: Department of Oncology, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Tumor Research and Therapy Center, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Han
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics* / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013270:Stomach / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D020672:I-kappa B Proteins; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; C000606204:NFKBIA protein, human; D016376:Oligonucleotides, Antisense; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D000072000:NF-KappaB Inhibitor alpha; D008156:Luciferases