title: Construction and in vitro characterization of three-dimensional silk fibroinchitosan scaffolds.
abstract: The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds. Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3- to fabricate the composite materials. To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated. Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time. Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed. Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Liaoning Institute of Dental Research.
authors.forename: Shuang
authors.initials: S
authors.lastname: Tong
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Xu
authors.forename: Zi-Mei
authors.initials: ZM
authors.lastname: Liu
authors.forename: Xu-Kai
authors.initials: XK
authors.lastname: Wang
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water
chemical_list: D001672:Biocompatible Materials; D014867:Water; D005349:Fibroins; D048271:Chitosan