title: Regulation of PLCβ2 by the electrostatic and mechanical properties of lipid bilayers.
abstract: Phosphoinositide-specific phospholipase C (PLC) is an important family of enzymes constituting a junction between phosphoinositide lipid signaling and the trans-membrane signal transduction processes that are crucial to many living cells. However, the regulatory mechanism of PLC is not yet understood in detail. To address this issue, activity studies were carried out using lipid vesicles in a model system that was specifically designed to study protein-protein and lipid-protein interactions in concert. Evidence was found for a direct interaction between PLC and the GTPases that mediate phospholipase activation. Furthermore, for the first time, the relationships between PLC activity and substrate presentation in lipid vesicles of various sizes, as well as lipid composition and membrane mechanical properties, were analyzed. PLC activity was found to depend upon the electrostatic potential and the stored curvature elastic stress of the lipid membranes.
authors.affiliation: Institute of Chemical Biology, Department of Chemical Biology, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.
authors.forename: Alessia
authors.initials: A
authors.lastname: Arduin
authors.affiliation: Institute of Chemical Biology, Department of Chemical Biology, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.
authors.forename: Piers R J
authors.initials: PR
authors.lastname: Gaffney
authors.affiliation: Institute of Chemical Biology, Department of Chemical Biology, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.
authors.forename: Oscar
authors.initials: O
authors.lastname: Ces
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004548:Elasticity; D004563:Electrochemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D054799:Phospholipase C beta / Q000737:chemistry*; D017368:Protein Prenylation; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction; D020744:rac GTP-Binding Proteins / Q000737:chemistry; D000097766:RAC2 GTP-Binding Protein
chemical_list: D004134:Dimyristoylphosphatidylcholine; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D054799:Phospholipase C beta; D020744:rac GTP-Binding Proteins; D000097766:RAC2 GTP-Binding Protein; C017251:1,2-oleoylphosphatidylcholine