title: Evaluation of the influence of salt processing on pharmacokinetics of psoralen and isopsoralen in Psoralea corylifolia L.
abstract: A sensitive, specific and rapid ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed to investigate pharmacokinetic properties of psoralen and isopsoralen, two compounds isolated from raw/salt-processed fruit of Psoralea corylifolia L. UHPLC-MS/MS was used with positive ion electrospray. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous solution and a gradient elution program at flow rate of 0.3 mL/min was applied. Multiple reaction monitoring mode was used for the quantification of psoralen, isopsoralen ([M + H](+) m/z 187.0 → m/z 131.0) and scoparone (m/z 207.0 → m/z 151.1). Scoparone served as an internal standard. The method was fully validated for its sensitivity, selectivity, stability, matrix effect and extraction recovery. The obtained results showed that salt-processed Buguzhi significantly promoted the absorption of psoralen and isopsoralen, and increased the bioavailability of these compounds.
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Qian qian
authors.initials: Qq
authors.lastname: Gao
authors.affiliation: Jiangsu Jumpcan Pharmaceutical Group Co., Ltd, Taizhou, 225441, China.
authors.forename: Cui ping
authors.initials: Cp
authors.lastname: Yan
authors.affiliation: Wuhu Pure Sunshine Natural Medicine Company Limited, Wuhu, 241000, China.
authors.forename: Zi sheng
authors.initials: Zs
authors.lastname: Xu
authors.affiliation: Nantong Hospital of Traditional Chinese Medicine, Nantong, 226001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Ze bin
authors.initials: Zb
authors.lastname: Weng
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Gen hua
authors.initials: Gh
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Liu jie
authors.initials: Lj
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Jia yu
authors.initials: Jy
authors.lastname: He
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Bao chang
authors.initials: Bc
authors.lastname: Cai
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Zhi peng
authors.initials: Zp
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Wei dong
authors.initials: Wd
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D005363:Ficusin / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D005638:Fruit / Q000737:chemistry; D011564:Furocoumarins / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D029907:Psoralea / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D012492:Salts / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000890:Anti-Infective Agents; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; D012492:Salts; C011659:angelicin; D005363:Ficusin
keywords: isopsoralen; pharmacokinetics; psoralen; salt processing; ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)