title: Fabrication of aligned, porous and conductive fibers and their effects on cell adhesion and guidance.
abstract: The potential applications of aligned, conductive electrospun fibers have been widely studied in anisotropic tissue regeneration. In this study, aligned porous poly L-lactic acid fibers were obtained with electrospinning, then polypyrrole nanoparticles (PPy NPs) were coated onto the porous fibers with oxidation polymerization to prepare electrically conductive fibers with about 1.24 μm of diameter, and their surface conductivity was about 50 mS. The results of L929 cell test showed that more than 55% of cells grew along the aligned porous fiber axis, confirming that the cell guidance of aligned porous fibers was better than that of non-porous fibers. The results of differentiated PC12 cells on porous fibers showed that the alignment degree of neurite outgrowth and average neurite length of the cells were 84% and 111 μm, respectively, which were larger than those on the non-porous fibers. A primary mechanism was proposed to explain effect of these pores on cell/neurite adhesion and orientation along the aligned porous fibers.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610065, China.
authors.forename: Anneng
authors.initials: A
authors.lastname: Yang
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610065, China. Electronic address: zbhuang@scu.edu.cn.
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610065, China.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610065, China.
authors.forename: Ximing
authors.initials: X
authors.lastname: Pu
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016716:PC12 Cells; D058105:Polymerization; D016062:Porosity; D051381:Rats
keywords: Aligned porous fibers; Anisotropic tissue engineering; Cell adhesion; Cell alignment