title: Electrospun Nanofibrous P(DLLA-CL) Balloons as Calcium Phosphate Cement Filled Containers for Bone Repair: in Vitro and in Vivo Studies.
abstract: The spinal surgeon community has expressed significant interest in applying calcium phosphate cement (CPC) for the treatment of vertebral compression fractures (VCFs) and minimizing its disadvantages, such as its water-induced collapsibility and poor mechanical properties, limiting its clinical use. In this work, novel biodegradable electrospun nanofibrous poly(d,l-lactic acid-ϵ-caprolactone) balloons (ENPBs) were prepared, and the separation, pressure, degradation, and new bone formation behaviors of the ENPBs when used as CPC-filled containers in vitro and in vivo were systematically analyzed and compared. CPC could be separated from surrounding bone tissues by ENPBs in vitro and in vivo. ENPB-CPCs (ENPBs serving as CPC-filled containers) exerted pressure on the surrounding bone microenvironment, which was enough to crush trabecular bone. Compared with the CPC implantation, ENPB-CPCs delayed the degradation of CPC (i.e., its water-induced collapsilibity). Finally, possible mechanisms behind the in vivo effects caused by ENPB-CPCs implanted into rabbit thighbones and pig vertebrae were proposed. This work suggests that ENPBs can be potentially applied as CPC-filled containers in vivo and provides an experimental basis for the clinical application of ENPBs for the treatment of VCFs. In addition, this work will be of benefit to the development of polymer-based medical implants in the future.
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , No. 25 Shifan Road, Jinan 200050, Shandong Province, People's Republic of China.
authors.forename: Xunwei
authors.initials: X
authors.lastname: Liu
authors.affiliation: National Engineering Research Center for Nanotechnology , No. 28 East Jiangchuang Road, Minhang District, Shanghai 200241, People's Republic of China.
authors.forename: Daixu
authors.initials: D
authors.lastname: Wei
authors.affiliation: National Engineering Research Center for Nanotechnology , No. 28 East Jiangchuang Road, Minhang District, Shanghai 200241, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
authors.affiliation: School of Materials Science and Engineering, Shanghai Jiao Tong University , No. 800 Dongchuang Road, Minhang District, Shanghai 200240, People's Republic of China.
authors.forename: Mengjia
authors.initials: M
authors.lastname: Ma
authors.affiliation: National Engineering Research Center for Nanotechnology , No. 28 East Jiangchuang Road, Minhang District, Shanghai 200241, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , No. 25 Shifan Road, Jinan 200050, Shandong Province, People's Republic of China.
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , No. 25 Shifan Road, Jinan 200050, Shandong Province, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , No. 25 Shifan Road, Jinan 200050, Shandong Province, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
authors.affiliation: National Engineering Research Center for Nanotechnology , No. 28 East Jiangchuang Road, Minhang District, Shanghai 200241, People's Republic of China.|School of Materials Science and Engineering, Shanghai Jiao Tong University , No. 800 Dongchuang Road, Minhang District, Shanghai 200240, People's Republic of China.
authors.forename: Dannong
authors.initials: D
authors.lastname: He
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001843:Bone Cements / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D001842:Bone and Bones / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D019736:Prostheses and Implants; D011817:Rabbits; D013124:Spinal Injuries / Q000473:pathology / Q000628:therapy; D013552:Swine; D014057:Tomography, X-Ray Computed
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D011091:Polyesters; C550605:poly(lactic acid-co-epsilon-caprolactone); C020243:calcium phosphate
keywords: calcium phosphate cement; electrospun nanofibrous P(DLLA-CL) balloons; in vitro; in vivo; pig vertebra; rabbit thighbone