title: High ROR2 expression in tumor cells and stroma is correlated with poor prognosis in pancreatic ductal adenocarcinoma.
abstract: RTK-like orphan receptor 2 (ROR2) is overexpressed in several cancers and has tumorigenic activity. However, the expression of ROR2 and its functional and prognostic significance have yet to be evaluated in pancreatic ductal adenocarcinoma (PDAC). Quantitative real-time polymerase chain reaction was used to characterize the expression of ROR2 mRNA in PDAC, corresponding peritumoral tissues, and PDAC cell lines. Immunohistochemical analysis with tissue microarrays was used to evaluate ROR2 expression in PDAC and to investigate the relationship of this expression to clinicopathological factors and prognosis. The expression of ROR2 mRNA and protein was significantly higher in PDAC than in normal pancreatic tissues. High cytoplasmic ROR2 expression in cancer cells was significantly associated with a primary tumor, distant metastasis, and TNM stage, and high stromal ROR2 expression was significantly associated with regional lymph node metastasis and TNM stage. The Kaplan-Meier method and Cox regression analyses showed that high ROR2 expression in tumor cytoplasm or stromal cells was significantly associated with malignant attributes and reduced survival in PDAC. We present strong evidence that ROR2 could be used as an indicator of poor prognosis and could represent a novel therapeutic target for PDAC.
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Fan
authors.affiliation: Surgical Comprehensive Laboratory, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1] Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China [2] Surgical Comprehensive Laboratory, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: 1] Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China [2] Surgical Comprehensive Laboratory, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000096:biosynthesis* / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C078717:ROR2 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors