title: Comparison of the Transcriptional Profiles of Melanocytes from Dark and Light Skinned Individuals under Basal Conditions and Following Ultraviolet-B Irradiation.
abstract: We analysed the whole-genome transcriptional profile of 6 cell lines of dark melanocytes (DM) and 6 of light melanocytes (LM) at basal conditions and after ultraviolet-B (UVB) radiation at different time points to investigate the mechanisms by which melanocytes protect human skin from the damaging effects of UVB. Further, we assessed the effect of different keratinocyte-conditioned media (KCM+ and KCM-) on melanocytes. Our results suggest that an interaction between ribosomal proteins and the P53 signaling pathway may occur in response to UVB in both DM and LM. We also observed that DM and LM show differentially expressed genes after irradiation, in particular at the first 6h after UVB. These are mainly associated with inflammatory reactions, cell survival or melanoma. Furthermore, the culture with KCM+ compared with KCM- had a noticeable effect on LM. This effect includes the activation of various signaling pathways such as the mTOR pathway, involved in the regulation of cell metabolism, growth, proliferation and survival. Finally, the comparison of the transcriptional profiles between LM and DM under basal conditions, and the application of natural selection tests in human populations allowed us to support the significant evolutionary role of MIF and ATP6V0B in the pigmentary phenotype.
authors.affiliation: Department of Genetics, Physical Anthropology and Animal Physiology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
authors.forename: Saioa
authors.initials: S
authors.lastname: López
authors.affiliation: Department of Zoology and Animal Cell Biology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Smith-Zubiaga
authors.affiliation: Department of Cell Biology and Histology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: García de Galdeano
authors.affiliation: Department of Cell Biology and Histology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain; BioCruces Health Research Institute, Cruces University Hospital, Cruces-Barakaldo, Bizkaia, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Boyano
authors.affiliation: Forensic Genetics Laboratory, Forensic Science Unit, Ertaintza-Basque Country Police, Erandio, Bizkaia, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: García
authors.affiliation: Dermatology Service, BioCruces Health Research Institute, Cruces University Hospital, Cruces-Barakaldo, Bizkaia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Gardeazábal
authors.affiliation: Department of Medicine, Jaume I University of Castellón, Castellón, Spain.
authors.forename: Conrado
authors.initials: C
authors.lastname: Martinez-Cadenas
authors.affiliation: Department of Genetics, Physical Anthropology and Animal Physiology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
authors.forename: Neskuts
authors.initials: N
authors.lastname: Izagirre
authors.affiliation: Department of Genetics, Physical Anthropology and Animal Physiology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: de la Rúa
authors.affiliation: Department of Genetics, Physical Anthropology and Animal Physiology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.
authors.forename: Santos
authors.initials: S
authors.lastname: Alonso
mesh_terms: D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008544:Melanocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D020411:Oligonucleotide Array Sequence Analysis; D025341:Principal Component Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects; D012867:Skin / Q000166:cytology / Q000378:metabolism; D012880:Skin Pigmentation / Q000235:genetics; D059467:Transcriptome / Q000528:radiation effects*; D014466:Ultraviolet Rays