title: Identification of Genetic Mutations in Human Lung Cancer by Targeted Sequencing.
abstract: Lung cancer remains the most prevalent malignancy and the primary cause of cancer-related deaths worldwide. Unique mutations patterns can be found in lung cancer subtypes, in individual cancers, or within a single tumor, and drugs that target these genetic mutations and signal transduction pathways are often beneficial to patients. In this study, we used the Ion Torrent AmpliSeq Cancer Panel to sequence 737 loci from 45 cancer-related genes and oncogenes to identify genetic mutations in 48 formalin-fixed, paraffin-embedded (FFPE) human lung cancer samples from Chinese patients. We found frequent mutations in EGFR, KRAS, PIK3CA, and TP53 genes. Moreover, we observed that a portion of the lung cancer samples harbored two or more mutations in these key genes. This study demonstrates the feasibility of using the Ion Torrent sequencing to efficiently identify genetic mutations in individual tumors for targeted lung cancer therapy.
authors.affiliation: Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Zhenrong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Chuanning
authors.initials: C
authors.lastname: Tang
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Jones
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lou
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Jiang
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Dong
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Guangchun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Zhishou
authors.initials: Z
authors.lastname: Dong
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Baishuai
authors.initials: B
authors.lastname: Guo
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: He
authors.initials: H
authors.lastname: Yan
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Yan
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Su
authors.affiliation: San Valley Biotechnology Inc., Beijing, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Nandakumar
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Xue F
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Si-Yi
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Deruo
authors.initials: D
authors.lastname: Liu
keywords: genetic mutations; lung cancer; targeted sequencing; targeted therapy