title: 5-Hydroxymethylfurfural modified rhodamine B dual-function derivative: Highly sensitive and selective optical detection of pH and Cu(2+).
abstract: A dual-function optical chemosensor (RBF) was designed and easily synthesized by condensation reaction of 5-Hydroxymethylfurfural and rhodamine B hydrazide. RBF exhibited highly sensitive, highly selective and quick response to acidic pH. The fluorescence intensity of RBF exhibited a more than 41-fold increase within the pH range from 7.50 to 3.73 with a pKa value of 5.02, which could be successfully applied to monitor intracellular pH in living PC12 cells and HeLa cells. Additionally, the spectroscopy of UV-Vis and EDTA-adding experiments indicated that RBF was a highly selective and reversible colorimetric chemosensor for Cu(2+) in Tris-HCl (10mM, pH=7.2) aqueous buffer solution as well as other metal ions had no obvious interference. Moreover, RBF has been successfully applied to detect Cu(2+) in real water samples.
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Enze
authors.initials: E
authors.lastname: Wang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China. Electronic address: zhouyanmei@henu.edu.cn.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Lanfang
authors.initials: L
authors.lastname: Pang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, United States.
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
authors.affiliation: Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Min
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
authors.forename: Tongsen
authors.initials: T
authors.lastname: Ma
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000032:analysis; D003124:Colorimetry / Q000379:methods; D003300:Copper / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D016716:PC12 Cells; D051381:Rats; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014867:Water / Q000032:analysis*
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D012235:Rhodamines; D014867:Water; C008046:5-hydroxymethylfurfural; D003300:Copper; D005662:Furaldehyde; C029773:rhodamine B
keywords: Colorimetric; Cu(2+); Fluorescent; Live-cells imaging; Rhodamine B; pH