title: Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.
abstract: This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
authors.forename: Maria Verônica
authors.initials: MV
authors.lastname: de Souza
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
authors.forename: Mariana Brettas
authors.initials: MB
authors.lastname: Silva
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
authors.forename: José de Oliveira
authors.initials: Jde O
authors.lastname: Pinto
authors.affiliation: Clínica Médica Planeta Animal, Rua Araguaia 30, Bairro São Mateus, 36025-240 Juiz de Fora, MG, Brazil.
authors.forename: Marianna Barros de Souza
authors.initials: MB
authors.lastname: Lima
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
authors.forename: Júlio
authors.initials: J
authors.lastname: Crepaldi
authors.affiliation: Laboratório de Patologia Experimental, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho 400, Bairro Chanadour, 35501-296 Divinópolis, MG, Brazil.
authors.forename: Gabriela Francine Martins
authors.initials: GF
authors.lastname: Lopes
authors.affiliation: Laboratório de Processamento de Tecido, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho 400, Bairro Chanadour, 35501-296 Divinópolis, MG, Brazil.
authors.forename: Hélio Batista
authors.initials: HB
authors.lastname: dos Santos
authors.affiliation: Laboratório de Patologia Experimental, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho 400, Bairro Chanadour, 35501-296 Divinópolis, MG, Brazil.
authors.forename: Rosy Iara Maciel de Azambuja
authors.initials: RI
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Processamento de Tecido, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho 400, Bairro Chanadour, 35501-296 Divinópolis, MG, Brazil.
authors.forename: Ralph Gruppi
authors.initials: RG
authors.lastname: Thomé
mesh_terms: D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006736:Horses; D007150:Immunohistochemistry; D053657:Platelet-Rich Plasma; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology
chemical_list: D003094:Collagen