title: Metabolic cross-talk between pathways of terpenoid backbone biosynthesis in spike lavender.
abstract: The metabolic cross-talk between the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways in developing spike lavender (Lavandula latifolia Med) was analyzed using specific inhibitors and on the basis of (13)C-labeling experiments. The presence of mevinolin (MEV), an inhibitor of the MVA pathway, at concentrations higher than 0.5 μM significantly reduced plant development, but not the synthesis of chlorophylls and carotenoids. On the other hand, fosmidomycin (FSM), an inhibitor of the MEP pathway, at concentrations higher than 20 μM blocked the synthesis of chlorophyll, carotenoids and essential oils, and significantly reduced stem development. Notably, 1.2 mM MVA could recover the phenotype of MEV-treated plants, including the normal growth and development of roots, and could partially restore the biosynthesis of photosynthetic pigments and, to a lesser extent, of the essential oils in plantlets treated with FSM. Spike lavender shoot apices were also used in (13)C-labeling experiments, where the plantlets were grown in the presence of [U-(13)C6]glucose. GC-MS-analysis of 1,8-cineole and camphor indicated that the C5-precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) of both monoterpenes are predominantly biosynthesized via the methylerythritol phosphate (MEP) pathway. However, on the basis of the isotopologue profiles, a minor contribution of the MVA pathway was evident that was increased in transgenic spike lavender plants overexpressing the 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), the first enzyme of the MVA pathway. Together, these findings provide evidence for a transport of MVA-derived precursors from the cytosol to the plastids in leaves of spike lavender.
authors.affiliation: Departamento de Biología Vegetal, ISIC/ERI de Biotecnología y Biomedicina BIOTECMED, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Mendoza-Poudereux
authors.affiliation: Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Germany.
authors.forename: Erika
authors.initials: E
authors.lastname: Kutzner
authors.affiliation: Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Huber
authors.affiliation: Departamento de Biología Vegetal, ISIC/ERI de Biotecnología y Biomedicina BIOTECMED, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Segura
authors.affiliation: Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eisenreich
authors.affiliation: Departamento de Biología Vegetal, ISIC/ERI de Biotecnología y Biomedicina BIOTECMED, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain. Electronic address: isabel.arrillaga@uv.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Arrillaga
mesh_terms: D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / Q000235:genetics / Q000378:metabolism; D027523:Lavandula / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D013729:Terpenes; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent
keywords: Essential oils; Fosmidomycin; Isotopologue profiling; Lamiaceae; Lavandula latifolia; Mevinolin; Spike lavender; Terpenoid biosynthesis