title: Soluble extract of soybean fermented with Aspergillus oryzae GB107 inhibits fat accumulation in cultured 3T3-L1 adipocytes.
abstract: OBJECTIVE
  This study was conducted to investigate the effects of fermented soybean (FS) extract on adipocyte differentiation and fat accumulation using cultured 3T3-L1 adipocytes.
  
  
  METHODS
  3T3-L1 adipocytes were treated with FS and nonfermented soybean (NFS) extract during differentiation for 10 days in vitro. Oil red O staining was performed and glycerol-3-phosphate dehydrogenase (GPDH) activity was measured for analysis of fat accumulation. Expressions of adipogenic genes were measured.
  
  
  RESULTS
  Soluble extract of soybean fermented with Aspergillus oryzae GB107 contained higher levels of low-molecular-weight protein than conventional soybean protein did. FS extract (50 µg/ml) inhibited adipocyte differentiation and fat accumulation during differentiation of 3T3-L1 preadipocytes for 10 days in vitro. Significantly lower GPDH activity was observed in differentiated adipocytes treated with the FS extract than those treated with NFS extract. Treatment with FS extract resulted in decreased expression levels of leptin, adiponectin, and adipogenin genes, which are associated with adipogenesis.
  
  
  CONCLUSIONS
  This report is the first to demonstrate that the water-soluble extract from FS inhibits fat accumulation and lipid storage in 3T3-L1 adipocytes. Thus, the soybean extract fermented with A. oryzae GB107 could be used to control lipid accumulation in adipocytes.
authors.affiliation: Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Kyoung-Ha
authors.initials: KH
authors.lastname: So
authors.affiliation: Faculty of Agriculture, Shinshu University, Nagano-ken 399-4598, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Faculty of Agriculture, Shinshu University, Nagano-ken 399-4598, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yonekura
authors.affiliation: Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Genebiotech Co., Seoul, 137-787 Korea.
authors.forename: Chan Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Katoh
authors.affiliation: Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Sang-Gun
authors.initials: SG
authors.lastname: Roh
keywords: 3T3-L1; Aspergillus oryzae; Fermented soybean; adipocyte; adipogenesis