title: Liposomes loaded with hydrophilic magnetite nanoparticles: Preparation and application as contrast agents for magnetic resonance imaging.
abstract: Magnetic fluid-loaded liposomes (MFLs) were fabricated using magnetite nanoparticles (MNPs) and natural phospholipids via the thin film hydration method followed by extrusion. The size distribution and composition of MFLs were studied using dynamic light scattering and spectrophotometry. The effective ranges of magnetite concentration in MNPs hydrosol and MFLs for contrasting at both T2 and T1 relaxation were determined. On T2 weighted images, the MFLs effectively increased the contrast if compared with MNPs hydrosol, while on T1 weighted images, MNPs hydrosol contrasting was more efficient than that of MFLs. In vivo magnetic resonance imaging (MRI) contrasting properties of MFLs and their effects on tumor and normal tissues morphology, were investigated in rats with transplanted renal cell carcinoma upon intratumoral administration of MFLs. No significant morphological changes in rat internal organs upon intratumoral injection of MFLs were detected, suggesting that the liposomes are relatively safe and can be used as the potential contrasting agents for MRI.
authors.affiliation: Saratov State University, 410012 Saratov, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: German
authors.affiliation: Saratov Medical State University, 410012 Saratov, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Navolokin
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
authors.forename: N R
authors.initials: NR
authors.lastname: Kuznetsova
authors.affiliation: Saratov Medical State University, 410012 Saratov, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Zuev
authors.affiliation: Saratov State University, 410012 Saratov, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Inozemtseva
authors.affiliation: Saratov State University, 410012 Saratov, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Anis'kov
authors.affiliation: Saratov State University, 410012 Saratov, Russia.
authors.forename: E K
authors.initials: EK
authors.lastname: Volkova
authors.affiliation: Saratov Medical State University, 410012 Saratov, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Bucharskaya
authors.affiliation: Saratov Medical State University, 410012 Saratov, Russia.
authors.forename: G N
authors.initials: GN
authors.lastname: Maslyakova
authors.affiliation: Bionanotechnology Lab, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Republic of Tatarstan, Russia.
authors.forename: R F
authors.initials: RF
authors.lastname: Fakhrullin
authors.affiliation: Saratov State University, 410012 Saratov, Russia; Saratov Medical State University, 410012 Saratov, Russia.
authors.forename: G S
authors.initials: GS
authors.lastname: Terentyuk
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Vodovozova
authors.affiliation: Saratov State University, 410012 Saratov, Russia. Electronic address: gorinda@mail.ru.
authors.forename: D A
authors.initials: DA
authors.lastname: Gorin
mesh_terms: D000818:Animals; D002292:Carcinoma, Renal Cell / Q000473:pathology; D003287:Contrast Media / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000473:pathology; D008081:Liposomes / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D009368:Neoplasm Transplantation; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003287:Contrast Media; D008081:Liposomes; D058185:Magnetite Nanoparticles
keywords: Contrast agent; In vivo contrasting; Magnetic liposomes; Magnetic resonance imaging; Magnetite nanoparticles