title: Bisphenol A increases BeWo trophoblast survival in stress-induced paradigms through regulation of oxidative stress and apoptosis.
abstract: Bisphenol A (BPA) is ubiquitous in the environment and is reported to be present at high concentrations in placental tissue, where its presence raises concerns over its potential to disrupt placental function. This report investigates how BPA interferes with the survival of human choriocarcinoma BeWo cells (a model of placental trophoblasts) under stress-induced paradigms reminiscent of pathways activated in placental development. These include conditions that promote oxidative stress (glutathione depletion) and apoptosis (serum withdrawal) or mimic hypoxia (HIF-1α accumulation via dimethyloxalylglycine treatment). Treatment of BeWo cells with BPA during stress-induced paradigms led to a consistent and significant increase in cell viability, with a concomitant increase in glutathione levels and a reduction in apoptosis. Assessment of the antioxidant capacity of BPA revealed its ability to quench reactive oxygen species and reduce the levels generated during glutathione and serum depletion. BPA was also able to reduce the activation of the antioxidant response element (ARE) through mediation of its activators, nuclear factor erythroid related factor family members (Nrf's). Indeed, the expression and nuclear translocation of Nrf2 (an important ARE activator) were impaired by BPA, while Nrf1 and Nrf3 expression levels were increased. Furthermore, BPA increased the levels of the anti-apoptotic proteins (Bcl-2 and Hsp70) and decreased HIF-1α levels during stress-induced conditions. Together, these results indicate that BPA inhibits trophoblast cell death under conditions of cellular stress. This could have implications on placental trophoblasts during development.
authors.affiliation: Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University , Nottingham NG11 8NS, U.K.
authors.forename: Muralitharan
authors.initials: M
authors.lastname: Ponniah
authors.affiliation: Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University , Nottingham NG11 8NS, U.K.
authors.forename: E Ellen
authors.initials: EE
authors.lastname: Billett
authors.affiliation: Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University , Nottingham NG11 8NS, U.K.
authors.forename: Luigi A
authors.initials: LA
authors.lastname: De Girolamo
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D000255:Adenosine Triphosphate; D001559:Benzhydryl Compounds; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D010636:Phenols; D017382:Reactive Oxygen Species; C497442:HIF1A protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds