title: Lipid nanocarriers based on natural oils with high activity against oxygen free radicals and tumor cell proliferation.
abstract: The development of nano-dosage forms of phytochemicals represents a significant progress of the scientific approach in the biomedical research. The aim of this study was to assess the effectiveness of lipid nanocarriers based on natural oils (grape seed oil, fish oil and laurel leaf oil) in counteracting free radicals and combating certain tumor cells. No drug was encapsulated in the nanocarriers. The cytotoxic effect exerted by bioactive nanocarriers against two tumor cells, MDA-MB 231 and HeLa cell lines, and two normal cells, L929 and B16 cell lines, was measured using the MTT assay, while oxidative damage was assessed by measuring the total antioxidant activity using chemiluminescence analysis. The best performance was obtained for nanocarriers based on an association of grape seed and laurel leaf oils, with a capacity to scavenge about 98% oxygen free radicals. A dose of nanocarriers of 5mg·mL(-1) has led to a drastic decrease in tumor cell proliferation even in the absence of an antitumor drug (e.g. about 50% viability for MDA-MB 231 cell line and 60% viability for HeLa cell line). A comparative survival profile of normal and tumor cells, which were exposed to an effective dose of 2.5mg·mL(-1) lipid nanocarriers, has revealed a death rate of 20% for normal B16 cells and of 40% death rate for MDA-MB 231 and HeLa tumor cells. The results in this study imply that lipid nanocarriers based on grape seed oil in association with laurel leaf oil could be a candidate to reduce the delivery system toxicity and may significantly improve the therapeutic efficacy of antitumor drugs in clinical applications.
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest, Romania.
authors.forename: I
authors.initials: I
authors.lastname: Lacatusu
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest, Romania.
authors.forename: N
authors.initials: N
authors.lastname: Badea
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest, Romania.
authors.forename: G
authors.initials: G
authors.lastname: Badea
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest, Romania.
authors.forename: O
authors.initials: O
authors.lastname: Oprea
authors.affiliation: Institute of Virusology "Stefan S. Nicolau", Center of Immunology, Bravu Road, No. 285, 030304 Bucharest, Romania.
authors.forename: M A
authors.initials: MA
authors.lastname: Mihaila
authors.affiliation: Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, 31030 Antakya, Hatay, Turkey.
authors.forename: D A
authors.initials: DA
authors.lastname: Kaya
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest, Romania. Electronic address: rl_stan2000@yahoo.com.
authors.forename: R
authors.initials: R
authors.lastname: Stan
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No 1, 011061 Bucharest, Romania.
authors.forename: A
authors.initials: A
authors.lastname: Meghea
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004304:Dosage Forms; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004304:Dosage Forms; D004337:Drug Carriers; D005395:Fish Oils; D005609:Free Radicals; D008055:Lipids; D010938:Plant Oils; D010100:Oxygen
keywords: Antioxidant activity; Functional nanocarriers; Grape seed oil; Laurel leaf oil; Tumor cell decline