title: Inactivation of the virulence factors from 2,3-butanediol-producing Klebsiella pneumoniae.
abstract: The microbiological production of 2,3-butanediol (2,3-BDO) has attracted considerable attention as an alternative way to produce high-value chemicals from renewable sources. Among the number of 2,3-BDO-producing microorganisms, Klebsiella pneumoniae has been studied most extensively and is known to produce large quantity of 2,3-BDO from a range of substrates. On the other hand, the pathogenic characteristics of the bacteria have limited its industrial applications. In this study, two major virulence traits, outer core LPS and fimbriae, were removed through homologous recombination from 2,3-BDO-producing K. pneumoniae 2242 to expand its uses to the industrial scale. The K. pneumoniae 2242 ∆wabG mutant strain was found to have an impaired capsule, which significantly reduced its ability to bind to the mucous layer and evade the phagocytic activity of macrophage. The association with the human ileocecal epithelial cell, HCT-8, and the bladder epithelial cell, T-24, was also reduced dramatically in the K. pneumoniae 2242 ∆fimA mutant strain that was devoid of fimbriae. However, the growth rate and production yield for 2,3-BDO were unaffected. The K. pneumoniae strains developed in this study, which are devoid of the major virulence factors, have a high potential for the efficient and sustainable production of 2,3-BDO.
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Duyen Thi Ngoc
authors.initials: DT
authors.lastname: Huynh
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: In-Hye
authors.initials: IH
authors.lastname: Seol
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Samuel
authors.initials: S
authors.lastname: Jung
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Lim
authors.affiliation: Department of Food Science and Technology, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Mi-Rae
authors.initials: MR
authors.lastname: Jo
authors.affiliation: Department of Food Science and Technology, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea.
authors.forename: Borim
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Wooki
authors.initials: W
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea. youngkim@khu.ac.kr.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea. youngkim@khu.ac.kr.
authors.forename: Young-Rok
authors.initials: YR
authors.lastname: Kim
mesh_terms: D001422:Bacterial Adhesion; D016667:Bacterial Capsules; D002072:Butylene Glycols / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D005285:Fermentation; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000648:ultrastructure; D005818:Genetic Engineering; D059765:Homologous Recombination; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D009154:Mutation; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; D008070:Lipopolysaccharides; D037521:Virulence Factors; C026978:2,3-butylene glycol
keywords: 2,3-Butanediol; Capsule; Fimbriae; Klebsiella pneumoniae; Lipopolysaccharides; Virulence