title: IgG Antibody Responses to Recombinant gp120 Proteins, gp70V1/V2 Scaffolds, and a CyclicV2 Peptide in Thai Phase I/II Vaccine Trials Using Different Vaccine Regimens.
abstract: RV144 correlates of risk analysis showed that IgG antibodies to gp70V1V2 scaffolds inversely correlated with risk of HIV acquisition. We investigated IgG antibody responses in RV135 and RV132, two ALVAC-HIV prime-boost vaccine trials conducted in Thailand prior to RV144. Both trials used ALVAC-HIV (vCP1521) at 0, 1, 3, and 6 months and HIV-1 gp120MNgD and gp120A244gD in alum (RV135) or gp120SF2 and gp120CM235 in MF59 (RV132) at 3 and 6 months. We assessed ELISA binding antibodies to the envelope proteins (Env) 92TH023, A244gD and MNgD, cyclicV2, and gp70V1V2 CaseA2 (subtype B) and 92TH023 (subtype CRF01_AE), and Env-specific IgG1 and IgG3. Antibody responses to gp120 A244gD, MNgD, and gp70V1V2 92TH023 scaffold were significantly higher in RV135 than in RV132. Antibodies to gp70V1V2 CaseA2 were detected only in RV135 vaccine recipients and IgG1 and IgG3 antibody responses to A244gD were significantly higher in RV135. IgG binding to gp70V1V2 CaseA2 and CRF01_AE scaffolds was higher with the AIDSVAX(®)B/E boost but both trials showed similar rates of antibody decline post-vaccination. MF59 did not result in higher IgG antibody responses compared to alum with the antigens tested. However, notable differences in the structure of the recombinant proteins and dosage used for immunizations may have contributed to the magnitude and specificity of IgG induced by the two trials.
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Nicos
authors.initials: N
authors.lastname: Karasavvas
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Chitraporn
authors.initials: C
authors.lastname: Karnasuta
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Hathairat
authors.initials: H
authors.lastname: Savadsuk
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Sirinan
authors.initials: S
authors.lastname: Madnote
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Dutsadee
authors.initials: D
authors.lastname: Inthawong
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Somsak
authors.initials: S
authors.lastname: Chantakulkij
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Surawach
authors.initials: S
authors.lastname: Rittiroongrad
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Sorachai
authors.initials: S
authors.lastname: Nitayaphan
authors.affiliation: 2 Faculty of Tropical Medicine, Mahidol University , Bangkok, Thailand .
authors.forename: Punnee
authors.initials: P
authors.lastname: Pitisuttithum
authors.affiliation: 3 Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand .
authors.forename: Prasert
authors.initials: P
authors.lastname: Thongcharoen
authors.affiliation: 4 Research Institute for Health Sciences, Chiang Mai University , Chiang Mai, Thailand .
authors.forename: Vinai
authors.initials: V
authors.lastname: Siriyanon
authors.affiliation: 5 Technical Resources International , Bethesda, Maryland.
authors.forename: Charla A
authors.initials: CA
authors.lastname: Andrews
authors.affiliation: 6 Novartis Vaccines and Diagnostics, Inc., Cambridge, Massachusetts.
authors.forename: Susan W
authors.initials: SW
authors.lastname: Barnett
authors.affiliation: 7 Sanofi Pasteur , Swiftwater, Pennsylvania.
authors.forename: James
authors.initials: J
authors.lastname: Tartaglia
authors.affiliation: 8 Global Solutions for Infectious Diseases (GSID) , South San Francisco, California.
authors.forename: Faruk
authors.initials: F
authors.lastname: Sinangil
authors.affiliation: 8 Global Solutions for Infectious Diseases (GSID) , South San Francisco, California.
authors.forename: Donald P
authors.initials: DP
authors.lastname: Francis
authors.affiliation: 9 U.S. Military HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, Maryland.|10 Henry M. Jackson Foundation for the Advancement of Military Medicine , Bethesda, Maryland.
authors.forename: Merlin L
authors.initials: ML
authors.lastname: Robb
authors.affiliation: 9 U.S. Military HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, Maryland.
authors.forename: Nelson L
authors.initials: NL
authors.lastname: Michael
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Viseth
authors.initials: V
authors.lastname: Ngauy
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Mark S
authors.initials: MS
authors.lastname: de Souza
authors.affiliation: 11 Military Malaria Research Program, Walter Reed Army Institute of Research , Silver Spring, Maryland.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Paris
authors.affiliation: 9 U.S. Military HIV Research Program, Walter Reed Army Institute of Research , Silver Spring, Maryland.|10 Henry M. Jackson Foundation for the Advancement of Military Medicine , Bethesda, Maryland.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Excler
authors.affiliation: 12 International Vaccine Institute , Seoul, Republic of Korea.
authors.forename: Jerome H
authors.initials: JH
authors.lastname: Kim
authors.affiliation: 1 Department of Retrovirology, Armed Forces Research Institute of Medical Sciences , Bangkok, Thailand .
authors.forename: Robert J
authors.initials: RJ
authors.lastname: O'Connell
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000097:blood*; D013785:Thailand; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic