title: Biopharmaceutical Characterization of Nebulized Antimicrobial Agents in Rats: 3. Tobramycin.
abstract: The aim of this study was to determine the biopharmaceutical characteristics of tobramycin (TOB) after nebulization in rats. TOB was administered by intravenous (i.v.) bolus or intratracheal nebulization (3 mg · kg(-1)), and concentrations were determined in plasma and epithelial lining fluid (ELF) by liquid chromatography-tandem mass spectrometry. The ratio of the TOB concentration in ELF to the plasma area under the curve (AUC) was more than 200 times as high after NEB as after i.v. bolus administration, indicating that TOB nebulization offers a biopharmaceutical advantage over i.v. administration.
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Marchand
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Grégoire
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Brillault
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lamarche
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Gobin
authors.affiliation: Inserm U1070, Pôle Biologie Santé, Poitiers, France Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France william.couet@univ-poitiers.fr.
authors.forename: William
authors.initials: W
authors.lastname: Couet
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D008297:Male; D009330:Nebulizers and Vaporizers; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry; D014031:Tobramycin / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D014031:Tobramycin