title: A cold-adapted, solvent and salt tolerant esterase from marine bacterium Psychrobacter pacificensis.
abstract: Lipolytic enzymes with unique physico-chemical characteristics are gaining more attention for their immense industrial importance. In this study, a novel lipolytic enzyme (Est11) was cloned from the genomic library of a marine bacterium Psychrobacter pacificensis. The enzyme was expressed in Escherichia coli and purified to homogeneity with molecular mass of 32.9kDa. The recombinant Est11 was able to hydrolyze short chain esters (C2-C8) and displayed an optimum activity against butyrate ester (C4). The optimal temperature and pH were 25°C and 7.5, respectively. Est11 retained more than 70% of its original activity at 10°C, suggesting that it was a cold-active esterase. The enzyme was highly active and stable at high concentration of NaCl (5M). Further, incubation with ethanol, isopropanol, propanediol, DMSO, acetonitrile, and glycerol rendered remarkable positive effects on Est11 activity. Typically, even at the concentration of 30% (v/v), ethanol, DMSO, and propanediol increased Est11 activity by 1.3, 2.0, and 2.4-folds, respectively. This new robust enzyme with remarkable properties like cold-adaptability, exceptional tolerance to salt and organic solvents provides us a promising candidate to meet the needs of some harsh industrial processes.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Gaobing
authors.initials: G
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan street, Wuhan 430070, China.
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan street, Wuhan 430070, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan street, Wuhan 430070, China.
authors.forename: Guojie
authors.initials: G
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan street, Wuhan 430070, China.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan street, Wuhan 430070, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan street, Wuhan 430070, China. Electronic address: lzd@mail.hzau.edu.cn.
authors.forename: Ziduo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D059001:Aquatic Organisms / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D003080:Cold Temperature; D004789:Enzyme Activation; D004795:Enzyme Stability; D004950:Esterases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D044096:Psychrobacter / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012997:Solvents / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D012997:Solvents; D004950:Esterases
keywords: Cold-adapted; Esterase; Halo-tolerant; Organic solvent-tolerant