title: CARD15 Gene Polymorphisms Are Associated with Tuberculosis Susceptibility in Chinese Holstein Cows.
abstract: Bovine tuberculosis (BTB) is a significant veterinary and financial problem in many parts of the world. Associations between specific host genes and susceptibility to mycobacterial infections, such as tuberculosis, have been reported in several species. The objective of this study was to identify and evaluate the relationship of single-nucleotide polymorphisms (SNPs) in the CARD15 gene with susceptibility to BTB in Chinese Holstein cows. DNA samples from 201 Chinese Holstein cows (103 cases and 98 controls) were collected from Kunming City, Yuxi City, and Dali City in China. SNPs in the CARD15 gene were assessed using polymerase chain reaction (PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Case-control association testing and statistical analysis identified six SNPs associated with susceptibility to BTB in Chinese Holstein cows. The frequency of genotypes C/T, A/G, A/G, A/G, C/T, and A/G in E4 (-37), 208, 1644, 1648, 1799, and E10 (+107), respectively, was significantly higher in cases than in controls, and also the alleles C, A, A, G, T, and A, respectively, were associated with a greater relative risk in cases than in controls. The distribution of two haplotypes, TGGACA and CAGACA, was significantly different between cases and controls. Overall, this case-control study suggested that E4 (-37)(C/T), 208(A/G), 1644(A/G), 1648(A/G), 1799(C/T), and E10 (+107)(A/G) in the CARD15 gene were significantly associated with susceptibility to BTB in Chinese Holstein cows and that haplotypes TGGACA and CAGACA could be used as genetic markers in marker-assisted breeding programs for breeding cows with high resistance to BTB.
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Shengkui
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Wenji
authors.initials: W
authors.lastname: Tu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Wengui
authors.initials: W
authors.lastname: Li
authors.affiliation: Center for Animal Disease Control and Prevention of Yunnan Province, Kunming, Yunnan Province, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Dong
authors.affiliation: Center for Animal Disease Control and Prevention of Yuxi City, Yuxi, Yunnan Province, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Hydraulic and Architectural Engineering, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chai
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Yifang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002681:China; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014380:Tuberculosis, Bovine / Q000235:genetics*
chemical_list: D053473:Nod2 Signaling Adaptor Protein