title: Gene transcription of pro-inflammatory cytokines and chemokines induced by IL-17A in canine keratinocytes.
abstract: BACKGROUND
  Pro-inflammatory cytokines and chemokines produced by activated keratinocytes play an important role in the pathogenesis of canine atopic dermatitis (AD) as well as human AD. Recent studies suggest that keratinocytes activated by IL-17A are involved in the pathogenesis of human AD. However, the role of IL-17A in canine keratinocytes is poorly understood.
  
  
  OBJECTIVE
  Interleukin-17A would induce the transcription of pro-inflammatory cytokines and chemokines in canine keratinocytes.
  
  
  METHODS
  The transcription levels of pro-inflammatory cytokines and chemokines were quantified in a canine keratinocyte cell line stimulated with recombinant canine (rc) IL-17A.
  
  
  RESULTS
  The transcription of GM-CSF, S100A8, IL-8 and IL-19 in cultured keratinocytes was significantly enhanced at 24 h after stimulation with rcIL-17A.
  
  
  CONCLUSIONS
  Keratinocytes activated by IL-17A have the ability to produce various pro-inflammatory cytokines and chemokines, suggesting that IL-17A may play a central role of the development of Th2-associated inflammation in canine AD.
authors.affiliation: The United Graduate School of Veterinary Sciences , Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Asahina
authors.affiliation: Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Kamishina
authors.affiliation: Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.affiliation: Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D005786:Gene Expression Regulation / Q000187:drug effects*; D020381:Interleukin-17 / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D016207:Cytokines; D020381:Interleukin-17