title: Micromonospora oryzaesp. nov., isolated from roots of upland rice.
abstract: An actinomycete strain, designated CP2R9-1T, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1Twas characterized as a member of the genusMicromonospora. meso-Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H4), MK-9(H6) and MK-10(H4). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1Twas closely related toMicromonospora haikouensis232617T(99.32 % similarity),Micromonospora carbonaceaDSM 43168T(99.18 %) andMicromonospora krabiensisMA-2T(99.16 %). Strain CP2R9-1Twas distinct from its closest relatives based on low levels of DNA-DNA relatedness (21.3 ± 0.1-41.7 ± 0.7 %) and phenotypic differences. The results presented in this study showed that strain CP2R9-1Trepresents a novel species of the genusMicromonospora, for which the nameMicromonospora oryzaesp. nov. is proposed. The type strain is CP2R9-1T( = BCC 67266T= NBRC 110007T).
authors.affiliation: Department of Biology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.|Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Chokchai
authors.initials: C
authors.lastname: Kittiwongwattana
authors.affiliation: Department of Biology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Dusanee
authors.initials: D
authors.lastname: Thanaboripat
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok 10520, Thailand.
authors.forename: Chamroon
authors.initials: C
authors.lastname: Laosinwattana
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok 10520, Thailand.
authors.forename: Prommart
authors.initials: P
authors.lastname: Koohakan
authors.affiliation: Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok 10520, Thailand.
authors.forename: Nonglak
authors.initials: N
authors.lastname: Parinthawong
authors.affiliation: Department of Biology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.|Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai