title: Short- and long-term effects of platelet-rich plasma upon healthy equine joints: Clinical and laboratory aspects.
abstract: This study aimed to verify whether transient inflammatory reactions incited by the administration of intra-articular platelet-rich plasma (PRP) affected joint components through short- and long-term in vivo evaluation of inflammatory biomarkers and extracellular matrix degradation products in synovial fluid. The effects of PRP were analyzed in a short phase protocol (SPP) and in a prolonged phase protocol (PPP), using saline-injected joints as controls. In the SPP, higher white blood cell counts and prostaglandin E2 and total protein concentrations were observed in the synovial fluid of PRP-treated joints (P < 0.05). There were no differences between the interleukin-1β, interleukin-1 receptor antagonist protein, tumor necrosis factor-α, chondroitin sulfate, or hyaluronic acid concentrations between PRP and saline injected joints. In the PPP, there were no differences in evaluated parameters between groups. PRP injection elicits a mild and self-limiting inflammatory response shortly after administration, without long-term deleterious effects on joint homeostasis.
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
authors.forename: Ana Paula L
authors.initials: AP
authors.lastname: Moraes
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
authors.forename: Juliana J
authors.initials: JJ
authors.lastname: Moreira
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Brossi
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
authors.forename: Thaís S L
authors.initials: TS
authors.lastname: Machado
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
authors.forename: Yara M
authors.initials: YM
authors.lastname: Michelacci
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
authors.forename: Raquel Y A
authors.initials: RY
authors.lastname: Baccarin
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006736:Horses / Q000502:physiology*; D007249:Inflammation / Q000378:metabolism / Q000662:veterinary*; D007270:Injections, Intra-Articular; D008297:Male; D053657:Platelet-Rich Plasma; D013582:Synovial Fluid / Q000737:chemistry
chemical_list: D015415:Biomarkers; D016207:Cytokines