title: Effect of high fat diet on paternal sperm histone distribution and male offspring liver gene expression.
abstract: Several studies have described phenotypic changes in the offspring of mice exposed to a variety of environmental factors, including diet, toxins, and stress; however, the molecular pathways involved in these changes remain unclear. Using a high fat diet (HFD)-induced obesity mouse model, we examined liver gene expression in male offspring and analyzed chromatin of paternal spermatozoa. We found that the hepatic mRNA level of 7 genes (out of 20 evaluated) was significantly altered in HFD male offspring compared to control mice, suggesting that phenotypic changes in the offspring depend on parental diet. We examined 7 imprinted loci in spermatozoa DNA from HFD-treated and control fathers by bisulfite sequencing, but did not detect changes in DNA methylation associated with HFD. Using chromatin immunoprecipitation followed by high-throughput sequencing, we found differential histone H3-occupancy at genes involved in the regulation of embryogenesis and differential H3K4me1-enrichment at transcription regulatory genes in HFD fathers vs. control mice. These results suggest that dietary exposure can modulate histone composition at regulatory genes implicated in developmental processes.
authors.affiliation: a Mouse Cancer Genetics Program; Center for Cancer Research; National Cancer Institute ; Frederick , MD USA.
authors.forename: Minoru
authors.initials: M
authors.lastname: Terashima
authors.forename: Samantha
authors.initials: S
authors.lastname: Barbour
authors.forename: Jianke
authors.initials: J
authors.lastname: Ren
authors.forename: Weishi
authors.initials: W
authors.lastname: Yu
authors.forename: Yixing
authors.initials: Y
authors.lastname: Han
authors.forename: Kathrin
authors.initials: K
authors.lastname: Muegge
mesh_terms: D000818:Animals; D019175:DNA Methylation / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D018392:Genomic Imprinting / Q000187:drug effects; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D017422:Sequence Analysis, DNA; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006657:Histones
keywords: DNA methylation; epigenetic inheritance, high fat diet; epigenetics; histone H; obesity