title: An improved method for HLA-B and -C supratyping.
abstract: A growing body of evidence links the analysis of the KIR genotype and the presence of their HLA-B and -C ligands to a wide repertoire of human diseases. We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I(80), Bw4T(80), Bw6 and HLA-C1 or -C2 supratype.
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy. Electronic address: robertobiassoni@ospedale-gaslini.ge.it.
authors.forename: R
authors.lastname: Biassoni
authors.affiliation: Human Virology Unit, Division of Immunology, Transplantation and Infectious Disease, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: M
authors.initials: S
authors.lastname: Malnati
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy.
authors.forename: I
authors.lastname: Vanni
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy.
authors.forename: A
authors.lastname: Raso
authors.affiliation: Human Virology Unit, Division of Immunology, Transplantation and Infectious Disease, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: F
authors.lastname: Sironi
authors.affiliation: Department of Health Sciences, University of Milano-Bicocca, Milano, Italy.
authors.forename: F
authors.lastname: Broccolo
authors.affiliation: Histocompatibility Laboratory, Galliera Hospital, Genova, Italy.
authors.forename: L
authors.lastname: Garbarino
authors.affiliation: Histocompatibility Laboratory, Galliera Hospital, Genova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mazzocco
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Montera
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy.
authors.forename: P
authors.lastname: Larghero
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy.
authors.forename: E
authors.lastname: Di Marco
authors.affiliation: Istituto Giannina Gaslini, Genova, Italy.
authors.forename: E
authors.lastname: Ugolotti
mesh_terms: D000483:Alleles; D001483:Base Sequence; D015235:HLA-B Antigens / Q000145:classification* / Q000235:genetics; D015236:HLA-C Antigens / Q000145:classification* / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D058889:Molecular Typing / Q000379:methods; D054340:Receptors, KIR / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015235:HLA-B Antigens; C042388:HLA-Bw4 antigen; C038652:HLA-Bw6 antigen; D015236:HLA-C Antigens; D054340:Receptors, KIR
keywords: Genotyping; HLA; HLA-B (Bw4/Bw6); HLA-C (C1/C2); KIR; NK cells; Pyrosequencing