title: Circulating miRNAs: Potential Novel Biomarkers for Hepatopathology Progression and Diagnosis of Schistosomiasis Japonica in Two Murine Models.
abstract: BACKGROUND
  Schistosomiasis remains a major public health issue, with an estimated 230 million people infected worldwide. Novel tools for early diagnosis and surveillance of schistosomiasis are currently needed. Elevated levels of circulating microRNAs (miRNAs) are commonly associated with the initiation and progression of human disease pathology. Hence, serum miRNAs are emerging as promising biomarkers for the diagnosis of a variety of human diseases. This study investigated circulating host miRNAs commonly associated with liver diseases and schistosome parasite-derived miRNAs during the progression of hepatic schistosomiasis japonica in two murine models.
  
  
  RESULTS
  Two mouse strains (C57BL/6 and BALB/c) were infected with a low dosage of Schistosoma japonicum cercariae. The dynamic patterns of hepatopathology, the serum levels of liver injury-related enzymes and the serum circulating miRNAs (both host and parasite-derived) levels were then assessed in the progression of schistosomiasis japonica. For the first time, an inverse correlation between the severity of hepatocyte necrosis and the level of liver fibrosis was revealed during S. japonicum infection in BALB/c, but not in C57BL/6 mice. The inconsistent levels of the host circulating miRNAs, miR-122, miR-21 and miR-34a in serum were confirmed in the two murine models during infection, which limits their potential value as individual diagnostic biomarkers for schistosomiasis. However, their serum levels in combination may serve as a novel biomarker to mirror the hepatic immune responses induced in the mammalian host during schistosome infection and the degree of hepatopathology. Further, two circulating parasite-specific miRNAs, sja-miR-277 and sja-miR-3479-3p, were shown to have potential as diagnostic markers for schistosomiasis japonica.
  
  
  CONCLUSIONS
  We provide the first evidence for the potential of utilizing circulating host miRNAs to indicate different immune responses and the severity of hepatopathology outcomes induced in two murine strains infected with S. japonicum. This study also establishes a basis for the early and cell-free diagnosis of schistosomiasis by targeting circulating schistosome parasite-derived miRNAs.
authors.affiliation: Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cai
authors.affiliation: Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia.
authors.forename: Geoffrey N
authors.initials: GN
authors.lastname: Gobert
authors.affiliation: Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia.
authors.forename: Hong
authors.initials: H
authors.lastname: You
authors.affiliation: Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia.
authors.forename: Mary
authors.initials: M
authors.lastname: Duke
authors.affiliation: Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia.
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
mesh_terms: D000818:Animals; D015415:Biomarkers; D005260:Female; D008099:Liver / Q000469:parasitology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000097:blood*; D012554:Schistosomiasis japonica / Q000097:blood* / Q000175:diagnosis / Q000469:parasitology* / Q000473:pathology
chemical_list: D015415:Biomarkers; D035683:MicroRNAs