title: The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores.
abstract: Accumulation of PLK1 at kinetochores is essential for chromosome alignment and segregation; however, the mechanism underlying PLK1 recruitment to kinetochores remains unresolved. The chromatin remodeller RSF1 tightly associates with centromere proteins, but its mitotic function is unknown. Here we show that RSF1 localizes at mitotic kinetochores and directly binds PLK1. RSF1 depletion disrupts localization of PLK1 at kinetochores; the C-terminal fragment of RSF1, which can bind PLK1, is sufficient to restore PLK1 localization. Moreover, CDK1 phosphorylates RSF1 at Ser1375, and this phosphorylation is necessary for PLK1 recruitment. Subsequently, PLK1 phosphorylates RSF1 at Ser1359, stabilizing PLK1 deposition. Importantly, RSF1 depletion mimicks the chromosome misalignment phenotype resulting from PLK1 knockdown; these defects are rescued by RSF1 S1375D or RSF1 S1359D but not RSF1 S1375A, showing a functional link between phosphorylation of RSF1 and chromosome alignment. Together, these data show that RSF1 is an essential centromeric component that recruits PLK1 to kinetochores and plays a crucial role in faithful cell division.
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-380, Korea.|Department of Biomedical Sciences, Graduate School of Ajou University, Suwon 443-380, Korea.
authors.forename: Ho-Soo
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-380, Korea.
authors.forename: Yong-Yea
authors.initials: YY
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-380, Korea.|Department of Biomedical Sciences, Graduate School of Ajou University, Suwon 443-380, Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Cho
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-380, Korea.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Chae
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-380, Korea.|Department of Biomedical Sciences, Graduate School of Ajou University, Suwon 443-380, Korea.
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Yoo
authors.affiliation: Department of Biomedical Sciences, Graduate School of Ajou University, Suwon 443-380, Korea.|Department of Microbiology, Ajou University School of Medicine, Suwon 443-380, Korea.
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Kwon
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
authors.forename: Chang-Woo
authors.initials: CW
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-380, Korea.|Department of Biomedical Sciences, Graduate School of Ajou University, Suwon 443-380, Korea.
authors.forename: Hyeseong
authors.initials: H
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D016203:CDC2 Protein Kinase; D018797:Cell Cycle Proteins / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D055786:Gene Knockout Techniques; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008938:Mitosis; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D016203:CDC2 Protein Kinase; D018797:Cell Cycle Proteins; D018844:Cyclin-Dependent Kinases; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000097869:Polo-Like Kinase 1; C452851:RSF1 protein, human; C528899:CDK1 protein, human