title: The effect of Rift Valley fever virus Clone 13 vaccine on semen quality in rams.
abstract: Rift Valley fever (RVF) is an arthropod-borne viral disease of importance in livestock and humans. Epidemics occur periodically in domestic ruminants. People in contact with infected livestock may develop disease that varies from mild flu-like symptoms to fatal viraemia. Livestock vaccination may assist in disease control. Rift Valley fever virus (RVFV) Clone 13 is a relatively new vaccine against RVF, derived from an avirulent natural mutant strain of RVFV, and has been shown to confer protective immunity against experimental infection with RVFV. The hypothesis tested in the current trial was that rams vaccinated with RVFV Clone 13 vaccine would not experience a reduction in semen quality (measured by evaluating the percentage progressively motile and percentage morphologically normal spermatozoa in successive ejaculates) relative to unvaccinated control animals. Ram lambs were screened for antibodies to RVFV using a serum neutralisation test. Animals without detectable antibodies (n = 23) were randomly allocated to either a test group (n = 12) or a control group (n = 11). Animals in the test group were vaccinated with RVFV Clone 13 vaccine. Daily rectal temperature measurements and weekly semen and blood samples were taken from all animals. Seven animals were eliminated from the statistical analysis because of potential confounding factors. Logistic regression analysis was performed on data gathered from the remaining animals to determine whether an association existed between animal group, rectal temperature and semen quality parameters. No correlation existed between the treatment group and values obtained for the semen quality parameters measured. There was no statistically significant post-vaccination decline in the percentage of live morphologically normal spermatozoa, or the percentage of progressively motile spermatozoa, either when assessed amongst all animals or when assessed within individual groups. A repeat study with a larger sample size and a more comprehensive pre-screening process may be indicated to avoid the inclusion of unsuitable animals.
authors.affiliation: Department of Production Animal Studies, University of Pretoria. geoff.brown@up.ac.za.
authors.forename: Geoff
authors.initials: G
authors.lastname: Brown
authors.forename: Estelle H
authors.initials: EH
authors.lastname: Venter
authors.forename: Paul
authors.initials: P
authors.lastname: Morley
authors.forename: Henry
authors.initials: H
authors.lastname: Annandale
mesh_terms: D000818:Animals; D001831:Body Temperature; D008297:Male; D012296:Rift Valley fever virus / Q000276:immunology*; D012661:Semen / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary; D034561:Sheep, Domestic / Q000502:physiology*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage / Q000009:adverse effects*
chemical_list: D014765:Viral Vaccines