title: Lipid Production of Heterotrophic Chlorella sp. from Hydrolysate Mixtures of Lipid-Extracted Microalgal Biomass Residues and Molasses.
abstract: This study investigated the feasibility of lipid production of Chlorella sp. from waste materials. Lipid-extracted microalgal biomass residues (LMBRs) and molasses were hydrolyzed, and their hydrolysates were analyzed. Five different hydrolysate mixture ratios (w/w) of LMBRs/molasses (1/0, 1/1, 1/4, 1/9, and 0/1) were used to cultivate Chlorella sp. The results showed that carbohydrate and protein were the two main compounds in the LMBRs, and carbohydrate was the main compound in the molasses. The highest biomass concentration of 5.58 g/L, Y biomass/sugars of 0.59 g/g, lipid productivity of 335 mg/L/day, and Y lipids/sugars of 0.25 g/g were obtained at the hydrolysate mixture ratio of LMBRs/molasses of 1/4. High C/N ratio promoted the conversion of sugars into lipids. The lipids extracted from Chlorella sp. shared similar lipid profile of soybean oil and is therefore a potential viable biodiesel feedstock. These results showed that Chlorella sp. can utilize mixed sugars and amino acids from LMBRs and molasses to accumulate lipids efficiently, thus reducing the cost of microalgal biodiesel production and improving its economic viability.
authors.affiliation: MOE Biomass Energy Research Center and College of Food Science and State Key Laboratory of Food Science, Nanchang University, Nanchang, Jiangxi, 330047, People's Republic of China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN, 55108, USA.|Institute of Microbiology, Chinese Academy of Sciences, A3 Datun Road., Chaoyang District, Beijing, 100101, People's Republic of China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: MOE Biomass Energy Research Center and College of Food Science and State Key Laboratory of Food Science, Nanchang University, Nanchang, Jiangxi, 330047, People's Republic of China.
authors.forename: Yiqin
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN, 55108, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Min
authors.affiliation: Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN, 55108, USA.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN, 55108, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: MOE Biomass Energy Research Center and College of Food Science and State Key Laboratory of Food Science, Nanchang University, Nanchang, Jiangxi, 330047, People's Republic of China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN, 55108, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Chen
authors.affiliation: MOE Biomass Energy Research Center and College of Food Science and State Key Laboratory of Food Science, Nanchang University, Nanchang, Jiangxi, 330047, People's Republic of China. ruanx001@umn.edu.|Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN, 55108, USA. ruanx001@umn.edu.
authors.forename: Roger
authors.initials: R
authors.lastname: Ruan
mesh_terms: D056804:Biofuels; D018533:Biomass; D049109:Cell Proliferation; D002708:Chlorella / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D052836:Heterotrophic Processes; D006868:Hydrolysis; D008055:Lipids / Q000096:biosynthesis* / Q000302:isolation & purification*; D058086:Microalgae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008965:Molasses; D014866:Waste Products
chemical_list: D056804:Biofuels; D008055:Lipids; D014866:Waste Products
keywords: Biodiesel; Heterotrophy; Lipid-extracted microalgal biomass residues; Microalga; Molasses