title: SFRP5 hepatic expression is associated with non-alcoholic liver disease in morbidly obese women.
abstract: OBJECTIVE
  Secreted frizzled-related protein 5 (SFRP5) was recently described as a new adipokine protective for hepatic steatosis and other obesity-related complications in the mouse model. To date, SFRP5 expression in non-alcoholic fatty liver disease (NAFLD) has not been fully assessed in humans. We measured circulating SFRP5 levels and its expression in liver and adipose tissue, and evaluated its association with NAFLD in morbidly obese women.
  
  
  METHODS
  Fifty-four morbidly obese women undergoing bariatric surgery were included in the study. Liver biopsies were used for histology and hepatic triglyceride content quantification. Circulating SFRP5 levels were measured through enzyme-linked immunoabsorbent assay, and SFRP5 expression was performed in hepatic and adipose tissue (subcutaneous and visceral).
  
  
  RESULTS
  Although circulating SFRP5 levels showed a tendency to decrease with NAFLD progression, no significant differences were observed among non-alcoholic steatosis, steatohepatitis, and control subjects. Hepatic SFRP5 expression showed a negative correlation with hepatic triglyceride content (r = -0.349, P = 0.016 for mRNA and r = -0.291, P = 0.040 for SRFP5 protein) and ALT serum levels (r = -0.437, P = 0.001 for SRFP5 protein). In addition, hepatic SFRP5 protein levels were significantly lower in NASH than in control subjects (P = 0.006).
  
  
  CONCLUSIONS
  This is the first study reporting an association of hepatic SFRP5 expression with NAFLD in humans.
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud. Facultad de Química, Universidad Nacional Autónoma de México. Instituto Nacional de Medicina Genómica (INMEGEN). Mexico City, Mexico.
authors.forename: Roxana
authors.initials: R
authors.lastname: Gutiérrez-Vidal
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud. Facultad de Química, Universidad Nacional Autónoma de México. Instituto Nacional de Medicina Genómica (INMEGEN). Mexico City, Mexico.
authors.forename: Joel
authors.initials: J
authors.lastname: Vega-Badillo
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud. Facultad de Química, Universidad Nacional Autónoma de México. Instituto Nacional de Medicina Genómica (INMEGEN). Mexico City, Mexico.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Reyes-Fermín
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud. Facultad de Química, Universidad Nacional Autónoma de México. Instituto Nacional de Medicina Genómica (INMEGEN). Mexico City, Mexico.
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Hernández-Pérez
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez (INCICh), Mexico City, Mexico.
authors.forename: Fausto
authors.initials: F
authors.lastname: Sánchez-Muñoz
authors.affiliation: Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional Zacatenco (CINVESTAV), Mexico City, Mexico.
authors.forename: Guadalupe S
authors.initials: GS
authors.lastname: López-Álvarez
authors.affiliation: Departamento de Gastroenterología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Mexico City, Mexico.
authors.forename: Elena
authors.initials: E
authors.lastname: Larrieta-Carrasco
authors.affiliation: Clínica Integral de Cirugía para la Obesidad y Enfermedades Metabólicas, Hospital General Dr. Rubén Leñero, Mexico City, Mexico.
authors.forename: Itzel
authors.initials: I
authors.lastname: Fernández-Silva
authors.affiliation: Unidad de Hígado, Fundación Clínica Médica Sur. Mexico City, Mexico.
authors.forename: Nahum
authors.initials: N
authors.lastname: Méndez-Sánchez
authors.affiliation: Departamento Fisiología de la Nutrición, INCMNSZ, Mexico City, Mexico.
authors.forename: Armando R
authors.initials: AR
authors.lastname: Tovar
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud. Facultad de Química, Universidad Nacional Autónoma de México. Instituto Nacional de Medicina Genómica (INMEGEN). Mexico City, Mexico.
authors.forename: Hugo
authors.initials: H
authors.lastname: Villamil-Ramírez
authors.affiliation: Banco de Sangre, INCICh, Mexico City, Mexico.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Mejía-Domínguez
authors.affiliation: Laboratorio de Enfermedades Cardiovasculares, INMEGEN, Mexico City, Mexico.
authors.forename: Teresa
authors.initials: T
authors.lastname: Villarreal-Molina
authors.affiliation: Departamento de Patología, INCMNSZ, Mexico City, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.affiliation: Clínica Integral de Cirugía para la Obesidad y Enfermedades Metabólicas, Hospital General Dr. Rubén Leñero, Mexico City, Mexico.
authors.forename: Francisco
authors.initials: F
authors.lastname: Campos-Pérez
authors.affiliation: Departamento de Endocrinología y Metabolismo, INCMNSZ, Mexico City, Mexico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Aguilar-Salinas
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud. Facultad de Química, Universidad Nacional Autónoma de México. Instituto Nacional de Medicina Genómica (INMEGEN). Mexico City, Mexico.
authors.forename: Samuel
authors.initials: S
authors.lastname: Canizales-Quinteros
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D050110:Bariatric Surgery; D001706:Biopsy; D003430:Cross-Sectional Studies; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000032:analysis* / Q000097:blood / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D050152:Intra-Abdominal Fat / Q000737:chemistry; D008099:Liver / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000175:diagnosis / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D009767:Obesity, Morbid / Q000150:complications* / Q000175:diagnosis / Q000601:surgery; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012720:Severity of Illness Index; D050151:Subcutaneous Fat / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis; D055815:Young Adult; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D005136:Eye Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D014280:Triglycerides; C118511:SFRP5 protein, human; D000094742:Secreted Frizzled-Related Proteins