title: Actinoplanes tropicalissp. nov. andActinoplanes cibodasensissp. nov., isolated from leaf litter.
abstract: Two actinomycete strains, designated LIPI11-2-Ac034Tand LIPI11-2-Ac042T, were isolated from leaf litter collected from Cibodas Botanical Garden, West Java, Indonesia. Phylogenetic analysis based on 16S rRNA gene sequences suggested that both isolates belong to the genusActinoplanes. These isolates were closely related toActinoplanes ferrugineusandActinoplanes durhamensiswith similarity values of 98.2 % and 97.7 % respectively, for strain LIPI11-2-Ac034T, and 99.0 % and 97.4-97.7 % respectively for strain LIPI11-2-Ac042T. Both isolates grew well on ISP 7 medium with brown soluble pigment production. Spores were motile and sporangia were irregular. The isolates containedmeso-diaminopimelic acid in cell-wall hydrolysates, and mannose, glucose and galactose in whole-cell hydrolysates. The predominant menaquinone of strain LIPI11-2-Ac034Twas MK-9(H4) while that of strain LIPI11-2-Ac042Twas MK-9(H6). The major cellular fatty acids were iso-C16 : 0, iso-C15 : 0and anteiso-C15 : 0for strain LIPI11-2-Ac034T, and iso-C16 : 0, anteiso-C15 : 0, iso-C15 : 0and anteiso-C17 : 0for strain LIPI11-2-Ac042T. Phosphatidylethanolamine was detected as the diagnostic polar lipid. The DNA G+C contents of strains LIPI11-2-Ac034Tand LIPI11-2-Ac042Twere 71.5 and 70.7 mol%, respectively. Based on the differential phenotypic characteristics and the results of DNA-DNA hybridization and phylogenetic analysis, it is proposed that strains LIPI11-2-Ac034Tand LIPI11-2-Ac042Trepresent two novel species of the genusActinoplanes, for which the namesActinoplanes tropicalissp. nov. (type strain LIPI11-2-Ac034T= InaCC A459T= NBRC 110973T) andActinoplanes cibodasensissp. nov. (type strain LIPI11-2-Ac042T= InaCC A458T= NBRC 110974T) are proposed.
authors.affiliation: Research Center for Biology, Indonesia Institute of Sciences (LIPI), Cibinong Science Center. Jl. Jakarta Bogor KM 46 Bogor, West Java, Indonesia.
authors.forename: Arif
authors.initials: A
authors.lastname: Nurkanto
authors.affiliation: Research Center for Biotechnology, Indonesia Institute of Sciences (LIPI), Cibinong Science Center. Jl. Jakarta Bogor KM 46 Bogor, West Java, Indonesia.
authors.forename: Puspita
authors.initials: P
authors.lastname: Lisdiyanti
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Research Center for Biotechnology, Indonesia Institute of Sciences (LIPI), Cibinong Science Center. Jl. Jakarta Bogor KM 46 Bogor, West Java, Indonesia.
authors.forename: Shanti
authors.initials: S
authors.lastname: Ratnakomala
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibata
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura