title: Autophagy protein 5 enhances the function of rat EPCs and promotes EPCs homing and thrombus recanalization via activating AKT.
abstract: Deep venous thrombosis (DVT) is one of the most common peripheral vascular diseases. The roles of bone marrow-derived endothelial progenitor cells (EPCs) on the recanalization of venous thrombosis has been suggested recently, while the underlying mechanisms are not completely understood. Our objective was to investigate the functions of autophagy protein 5 (ATG5) in rat EPCs and its potential application in DVT. We have found that silencing of ATG5 or pharmacological suppression of ATG5 in rat EPCs reduces both the migration and psudotube formation under hypoxia in vitro. In line, overexpression of ATG5 significantly enhances the EPCs migration and psudotube formation capabilities. More importantly, injection of EPCs that stably express ATG5 increases EPC homing to the ischemic site and promotes thrombus recanalization in a rat DVT model in vivo. Mechanistically, we have shown that ATG5 overexpression enhances psudotube formation via the activation of AKT. These findings suggest that ATG5-AKT signaling plays an essential role in EPC migration and psudotube formation. Regulation of ATG5-AKT signaling may provide a potential novel therapy for DVT.
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Ling-Shang
authors.initials: LS
authors.lastname: Kong
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Qian
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Du
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Yu
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China. Electronic address: hunan1613@126.com.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Li
mesh_terms: D000818:Animals; D000071187:Autophagy-Related Protein 5; D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D066253:Vascular Remodeling; D020246:Venous Thrombosis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*
chemical_list: C545865:Atg5 protein, rat; D000071187:Autophagy-Related Protein 5; D011506:Proteins; D051058:Oncogene Protein v-akt
keywords: Autophagy protein 5 (ATG5); Endothelial progenitor cells (EPC); Migration; Psudotube formation; Thrombus recanalization