title: Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells; a New Insight into Amino Acid Profiles.
abstract: BACKGROUND
  The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood.
  
  
  METHODS
  In the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes.
  
  
  RESULTS
  In optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient culture media) and 55% (for a high-nutrient culture media) were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium.
  
  
  CONCLUSIONS
  The amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells.
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Davami
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.|Science and Research Branch, Islamic Azad University of Arak, Markazi Province, Iran.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Eghbalpour
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Nematollahi
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Barkhordari
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Fereidoun
authors.initials: F
authors.lastname: Mahboudi
mesh_terms: D000818:Animals; D016466:CHO Cells; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000494:pharmacology*; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D010461:Peptones / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D010461:Peptones
keywords: CHO cells; Culture media; Peptones; Recombinant proteins