title: Optimization of bead milling parameters for the cell disruption of microalgae: process modeling and application to Porphyridium cruentum and Nannochloropsis oculata.
abstract: A study of cell disruption by bead milling for two microalgae, Nannochloropsis oculata and Porphyridium cruentum, was performed. Strains robustness was quantified by high-pressure disruption assays. The hydrodynamics in the bead mill grinding chamber was studied by Residence Time Distribution modeling. Operating parameters effects were analyzed and modeled in terms of stress intensities and stress number. RTD corresponded to a 2 CSTR in series model. First order kinetics cell disruption was modeled in consequence. Continuous bead milling was efficient for both strains disruption. SI-SN modeling was successfully adapted to microalgae. As predicted by high pressure assays, N. oculata was more resistant than P. cruentum. The critical stress intensity was twice more important for N. oculata than for P. cruentum. SI-SN modeling allows the determination of operating parameters minimizing energy consumption and gives a scalable approach to develop and optimize microalgal disruption by bead milling.
authors.affiliation: Université de Nantes, CNRS, GEPEA, UMR-CNRS 6144, bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Montalescot
authors.affiliation: AlgoSource Technologies, 37 Bd de l'Université, 44600 Saint-Nazaire, France.
authors.forename: T
authors.initials: T
authors.lastname: Rinaldi
authors.affiliation: Université de Nantes, CNRS, GEPEA, UMR-CNRS 6144, bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Touchard
authors.affiliation: AlgoSource Technologies, 37 Bd de l'Université, 44600 Saint-Nazaire, France.
authors.forename: S
authors.initials: S
authors.lastname: Jubeau
authors.affiliation: Université de Nantes, CNRS, GEPEA, UMR-CNRS 6144, bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Frappart
authors.affiliation: Université de Nantes, CNRS, GEPEA, UMR-CNRS 6144, bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Jaouen
authors.affiliation: Université de Nantes, CNRS, GEPEA, UMR-CNRS 6144, bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France; UBS Université de Bretagne Sud, LIMATB, rue de Saint-Maudé, BP 92116, 56321 Lorient, France.
authors.forename: P
authors.initials: P
authors.lastname: Bourseau
authors.affiliation: Université de Nantes, CNRS, GEPEA, UMR-CNRS 6144, bd de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Marchal
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D057446:Hydrodynamics; D058086:Microalgae / Q000737:chemistry / Q000166:cytology*; D008962:Models, Theoretical; D044665:Porphyridium / Q000737:chemistry / Q000166:cytology*; D011312:Pressure; D058009:Stramenopiles / Q000737:chemistry / Q000166:cytology*
keywords: Bead mill; Cell disruption; Nannochloropsis oculata; Porphyridium cruentum; Stress model