title: [Effect of Echinococcus multilocularis Cyst Fluid on the Expression of Five MAPK-pathway Genes of Rat Hepatic Stellate Cells].
abstract: OBJECTIVE
  To investigate the effect of Echinococcus multilocularis cyst fluid on five MAPK (mitogen-activated protein kinase)-pathway genes of rat hepatic stellate cell.
  
  
  METHODS
  Rat hepatic stellate cell line, HSC-T6 cells were co-cultured with different protein concentrations of E. multilocularis cyst fluid (0.01, 0.025, 0.05, 0.1, 0.2, 0.4, 0.9, 1.7, 3.4, 6.8, and 13.5 mg/ml) for 24 h. HSC-T6 cells cultured with complete medium served as control group. The morphological change of cells was observed under the microscope. The expression of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and mitogen-activated protein kinase(p38) in HSC-T6 cells was detected by real time fluorescent quantitative PCR.
  
  
  RESULTS
  After co-cultured for 24 h, most HSC-T6 cells in 13.5 mg/ml group shrank as a precursor to slough off; In 6.8 mg/ml group, some HSC-T6 cells shrank and changed to long fusiform shape with many slender pseudopodia; In 3.4 mg/ml group, most HSC-T6 cells showed as adherent cells with an irregular polygon shape, formed a sheet with short pseudopodia. There was no difference in cell morphology between < 1.7 mg/ml groups and control group. When the protein concentration was above 1.7 mg/ml, the mRNA level of ERK1/2, JNK1/2, and P38 increased significantly increased. In 6.8 mg/ml cyst fluid group, the mRNA level of ERK1/2, JNK1/2, and P38 was higher than that of the control (P < 0.05).
  
  
  CONCLUSIONS
  6.8 mg/ml Echinococcus multilocularis cyst fluid can have a significant impact on mRNA levels of ERK1/2, JNK1/2 and p38 in rat hepatic stellate cells.
authors.forename: Bin
authors.initials: B
authors.lastname: Ren
authors.forename: Hai-ning
authors.initials: HN
authors.lastname: Fan
authors.forename: Yong
authors.initials: Y
authors.lastname: Deng
authors.forename: Hai-jiu
authors.initials: HJ
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ren
mesh_terms: D000818:Animals; D002460:Cell Line; D020420:Cyst Fluid; D048210:Echinococcus multilocularis; D055166:Hepatic Stellate Cells; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases; D012333:RNA, Messenger; D051381:Rats
chemical_list: D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases