title: Inhibitory Effects of PEI-RGD/125I-(αv) ASODN on Growth and Invasion of HepG2 Cells.
abstract: BACKGROUND
  To investigate the in vitro inhibitory effects of PEI-RGD/125I-(αv)ASODN (PEI, polyethylenimine; RGD, Arg-Gly-Asp; ASODN, antisense oligodeoxynucleotide) on the growth and invasion of HepG2 cells.
  
  
  METHODS
  ASODN of the integrin αv-subunit was marked with 125I and underwent complexation with PEI-RGD, a PEI derivative. Next, PEI-RGD/125I-(αv) ASODN was introduced into HepG2 cells via receptor-mediated transfection, and its inhibition rate on HepG2 cell growth was tested using the methyl thiazolyl tetrazolium (MTT) method. The effects of PEI-RGD/125I-(αv) ASODN on HepG2 cell invasion ability were evaluated using the Boyden chamber assay.
  
  
  RESULTS
  1) The 125I marking rate of (αv) ASODN was 73.78±4.09%, and the radiochemical purity was 96.68±1.38% (greater than 90% even after a 48-h incubation period at 37°C), indicating high stability. 2) The cytotoxicity assays showed that the cell inhibition rates did not differ significantly between the PEI-RGD/125I-(αv)ASODN group and the PEI-RGD/(αv) ASODN group, but they were both significantly higher than in the other groups and were positively correlated (r=0.879) with the dosage within a certain range. 3) The invasion assays showed that the inhibition rate was significantly greater in the PEI-RGD/125I-(αv) ASODN group compared to the other groups.
  
  
  CONCLUSIONS
  PEI-RGD/125I-(αv) ASODN can efficiently inhibit the growth and proliferation of HepG2 cells and can also weaken their invasive ability.
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Haidong
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Blood Transfusion, Changhai Hospital, Second Military Medical University, Shanghai, China (mainland).
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Xueyu
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Shidong
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Lv
mesh_terms: D001483:Base Sequence; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D056945:Hep G2 Cells; D006801:Humans; D039564:Integrin alphaV / Q000235:genetics*; D007457:Iodine Radioisotopes / Q000008:administration & dosage*; D009361:Neoplasm Invasiveness; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage* / Q000235:genetics*; D009842:Oligopeptides / Q000008:administration & dosage*; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: D039564:Integrin alphaV; D007457:Iodine Radioisotopes; D020319:Oligodeoxyribonucleotides, Antisense; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D011094:Polyethyleneimine