title: Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: a multidisciplinary study of peritoneal carcinomatosis.
abstract: In general, detection of peritoneal carcinomatosis (PC) occurs at the late stage when there is no treatment option. In the present study, we designed novel drug delivery systems that are functionalized with anti-CD133 antibodies. The C1, C2 and C3 complexes with cisplatin were introduced into nanotubes, either physically or chemically. The complexes were reacted with anti-CD133 antibody to form the labeled product of A0-o-CX-chem-CD133. Cytotoxicity screening of all the complexes was performed on CHO cells. Data showed that both C2 and C3 Pt-complexes are more cytotoxic than C1. Flow-cytometry analysis showed that nanotubes conjugated to CD133 antibody have the ability to target cells expressing the CD133 antigen which is responsible for the emergence of resistance to chemotherapy and disease recurrence. The shortest survival rate was observed in the control mice group (K3) where no hyperthermic intraperitoneal chemotherapy procedures were used. On the other hand, the longest median survival rate was observed in the group treated with A0-o-C1-chem-CD133. In summary, we designed a novel drug delivery system based on carbon nanotubes loaded with Pt-prodrugs and functionalized with anti-CD133 antibodies. Our data demonstrates the effectiveness of the new drug delivery system and provides a novel therapeutic modality in the treatment of melanoma.
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Nowacki
authors.affiliation: Physicochemistry of Carbon Materials Research Group, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Poland.|Invest-Tech, Research and Development Center, Toruń, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Wisniewski
authors.affiliation: Physicochemistry of Carbon Materials Research Group, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Werengowska-Ciecwierz
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environment Protection, Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Roszek
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environment Protection, Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czarnecka
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Łakomska
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kloskowski
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Tyloch
authors.affiliation: Department of Pediatric Hematology and Oncology, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Debski
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.|Chair of Dermatology Department, Faculty of Medicine, Nicolaus Copernicus University, Toruń, Sexually Transmitted Diseases and Immunodermatology, Bydgoszcz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pietkun
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pokrywczynska
authors.affiliation: Chair of Dermatology Department, Faculty of Medicine, Nicolaus Copernicus University, Toruń, Sexually Transmitted Diseases and Immunodermatology, Bydgoszcz, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Grzanka
authors.affiliation: Chair of Dermatology Department, Faculty of Medicine, Nicolaus Copernicus University, Toruń, Sexually Transmitted Diseases and Immunodermatology, Bydgoszcz, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Czajkowski
authors.affiliation: Department of Medical Biology, University of Bydgoszcz, Poland.
authors.forename: Gerard
authors.initials: G
authors.lastname: Drewa
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Jundziłł
authors.affiliation: Department of Biochemistry, The University of Texas Health Science Center, San Antonio, TX, USA.
authors.forename: Joseph K
authors.initials: JK
authors.lastname: Agyin
authors.affiliation: Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio, TX, USA.|Department of Geriatric, South Texas Veterans Health System, The University of Texas Health Science Center, San Antonio, TX, USA.
authors.forename: Samy L
authors.initials: SL
authors.lastname: Habib
authors.affiliation: Physicochemistry of Carbon Materials Research Group, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Poland.
authors.forename: Artur P
authors.initials: AP
authors.lastname: Terzyk
authors.affiliation: Chair of Regenerative Medicine, Tissue Engineering Department, Ludwik Rydygier's Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland.|Urology Department, Nicolaus Copernicus Hospital in Toruń, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology; D002945:Cisplatin / Q000008:administration & dosage* / Q000737:chemistry*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D016503:Drug Delivery Systems / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology; D006979:Hyperthermia, Induced / Q000379:methods*; D000922:Immunotoxins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D007274:Injections, Intraperitoneal; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D010534:Peritoneal Neoplasms / Q000188:drug therapy / Q000628:therapy*; D015996:Survival Rate
chemical_list: D000071916:AC133 Antigen; D000906:Antibodies; D015703:Antigens, CD; D006023:Glycoproteins; D000922:Immunotoxins; D037742:Nanotubes, Carbon; D010455:Peptides; C000606024:Prom1 protein, mouse; D002945:Cisplatin
keywords: carcinomatosis; hyperthermic intraperitoneal chemotherapy; intraperitoneal perfusion and nanovehicles; palliative