title: Magnetic hyaluronic acid nanospheres via aqueous Diels-Alder chemistry to deliver dexamethasone for adipose tissue engineering.
abstract: Biopolymer-based nanospheres have great potential in the field of drug delivery and tissue regenerative medicine. In this work, we present a flexible way to conjugate a magnetic hyaluronic acid (HA) nanosphere system that are capable of vectoring delivery of adipogenic factor, e.g. dexamethasone, for adipose tissue engineering. Conjugation of nanospheres was established by aqueous Diels-Alder chemistry between furan and maleimide of HA derivatives. Simultaneously, a furan functionalized dexamethasone peptide, GQPGK, was synthesized and covalently immobilized into the nanospheres. The magnetic HA nanospheres were fabricated by encapsulating super-paramagnetic iron oxide nanoparticles, which exhibited quick magnetic sensitivity. The aqueous Diels-Alder chemistry made nanospheres high binding efficiency of dexamethasone, and the vectoring delivery of dexamethasone could be easily controlled by a external magnetic field. The potential application of the magnetic HA nanospheres on vectoring delivery of adipogenic factor was confirmed by co-culture of human adipose-derived stem cells (ASCs). In vitro cytotoxicity tests demonstrated that incorporation of dexamethasone into magnetic HA nanospheres showed high efficiency to promote ASCs viabilities, in particular under a magnetic field, which suggested a promising future for adipose regeneration applications.
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jia
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Huinan
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Miao
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xing
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Bohong
authors.initials: B
authors.lastname: Jiang
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Qifan
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Weikang
authors.initials: W
authors.lastname: Bao
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qian
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Jionglu
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xing
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: hptan@njust.edu.cn.
authors.forename: Huaping
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Orthopaedics, Jinling Hospital, Nanjing 210002, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Ling
authors.affiliation: Department of Orthopaedics, Jinling Hospital, Nanjing 210002, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D016503:Drug Delivery Systems; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008280:Magnetics; D054118:Nanospheres / Q000737:chemistry*; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D003907:Dexamethasone; D006820:Hyaluronic Acid
keywords: Biopolymers; Drug delivery; Magnetic nanoparticles; Nanospheres; Tissue engineering