title: Supplementary heat-killed Lactobacillus reuteri GMNL-263 ameliorates hyperlipidaemic and cardiac apoptosis in high-fat diet-fed hamsters to maintain cardiovascular function.
abstract: Obesity and hyperlipidaemia increase the risk of CVD. Some strains of probiotics have been suggested to have potential applications in cardiovascular health by lowering serum LDL-cholesterol. In this work, high-fat diet-induced hyperlipidaemia in hamsters was treated with different doses (5×108 and 2·5×109 cells/kg per d) of heat-killed Lactobacillus reuteri GMNL-263 (Lr263) by oral gavage for 8 weeks. The serum lipid profile analysis showed that LDL-cholesterol and plasma malondialdehyde (P-MDA) were reduced in the GMNL-263 5×108 cells/kg per d treatment group. Total cholesterol and P-MDA were reduced in the GMNL-263 2·5×109 cells/kg per d treatment group. In terms of heart function, the GMNL-263 2·5×109 cells/kg per d treatments improved the ejection fraction from 85·71 to 91·81 % and fractional shortening from 46·93 to 57·92 % in the high-fat diet-fed hamster hearts. Moreover, the GMNL-263-treated, high-fat diet-fed hamster hearts exhibited reduced Fas-induced myocardial apoptosis and a reactivated IGF1R/PI3K/Akt cell survival pathway. Interestingly, the GMNL-263 treatments also enhanced the heat-shock protein 27 expression in a dose-dependent manner, but the mechanism for this increase remains unclear. In conclusion, supplementary heat-killed L. reuteri GMNL-263 can slightly reduce serum cholesterol. The anti-hyperlipidaemia effects of GMNL-263 may reactivate the IGF1R/PI3K/Akt cell survival pathway and reduce Fas-induced myocardial apoptosis in high-fat diet-fed hamster hearts.
authors.affiliation: 1Graduate Institute of Basic Medical Science,China Medical University,Taichung-40402,Taiwan,ROC.
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Ting
authors.affiliation: 2Department of Biological Science and Technology,China Medical University,Taichung-40402,Taiwan,ROC.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Kuo
authors.affiliation: 3Laboratory of Exercise Biochemistry,Department of Sports Sciences,University of Taipei,Taipei-11153,Taiwan,ROC.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Kuo
authors.affiliation: 4Department of Pathology,Changhua Christian Hospital,Changhua-50002,Taiwan,ROC.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Yeh
authors.affiliation: 6Department of Nursing,Mei Ho University,Pingtung-91202,Taiwan,ROC.
authors.forename: Chia-Yao
authors.initials: CY
authors.lastname: Shen
authors.affiliation: 7Research and Development Department,GenMont Biotech Incorporation,Tainan-74144,Taiwan,ROC.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Chen
authors.affiliation: 8Chinese Medicine Department,China Medical University Beigang Hospital,Beigang-65152,Taiwan.
authors.forename: Tsung-Jung
authors.initials: TJ
authors.lastname: Ho
authors.affiliation: 9Department of Biotechnology,Bharathiar University,Coimbatore-461046,India.
authors.forename: Vijaya Padma
authors.initials: VP
authors.lastname: Viswanadha
authors.affiliation: 7Research and Development Department,GenMont Biotech Incorporation,Tainan-74144,Taiwan,ROC.
authors.forename: Yi-Hsing
authors.initials: YH
authors.lastname: Chen
authors.affiliation: 1Graduate Institute of Basic Medical Science,China Medical University,Taichung-40402,Taiwan,ROC.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D002784:Cholesterol / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D006224:Cricetinae; D059305:Diet, High-Fat; D006321:Heart / Q000503:physiopathology*; D006358:Hot Temperature; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D052200:Limosilactobacillus reuteri; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009206:Myocardium / Q000473:pathology*; D019936:Probiotics; D015398:Signal Transduction
chemical_list: D008078:Cholesterol, LDL; D008315:Malondialdehyde; D002784:Cholesterol
keywords: %FS fractional shortening; EF ejection fraction; HSP27 heat-shock protein 27; Heart protection; Hyperlipidaemia; LDL-cholesterol; Lactobacillus reuteri GMNL-263 (Lr263); P-MDA plasma malondialdehyde