title: Probing Site-Specific Structural Information of Peptides at Model Membrane Interface In Situ.
abstract: Isotope labeling is a powerful technique to probe detailed structures of biological molecules with a variety of analytical methods such as NMR and vibrational spectroscopies. It is important to obtain molecular structural information on biological molecules at interfaces such as cell membranes, but it is challenging to use the isotope labeling method to study interfacial biomolecules. Here, by individually (13)C═(16)O labeling ten residues of a peptide, Ovispirin-1, we have demonstrated for the first time that a site-specific environment of membrane associated peptide can be probed by the submonolayer surface sensitive sum frequency generation (SFG) vibrational spectroscopy in situ. With the peptide associated with a single lipid bilayer, the sinusoidal trend of the SFG line width and peak-center frequency suggests that the peptide is located at the interface beneath the lipid headgroup region. The constructive interferences between the isotope labeled peaks and the main peptide amide I peak contributed by the unlabeled components were used to determine the membrane orientation of the peptide. From the SFG spectral peak-center frequency, line width, and polarization dependence of the isotope labeled units, we deduced structural information on individual units of the peptide associated with a model cell membrane. We also performed molecular dynamics (MD) simulations to understand peptide-membrane interactions. The physical pictures described by simulation agree well with the SFG experimental result. This research demonstrates the feasibility and power of using isotope labeling SFG to probe molecular structures of interfacial biological molecules in situ in real time.
authors.affiliation: †Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
authors.forename: Bei
authors.initials: B
authors.lastname: Ding
authors.affiliation: †Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
authors.forename: Afra
authors.initials: A
authors.lastname: Panahi
authors.affiliation: ‡Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53703, United States.
authors.forename: Jia-Jung
authors.initials: JJ
authors.lastname: Ho
authors.affiliation: ‡Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53703, United States.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Laaser
authors.affiliation: †Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Brooks
authors.affiliation: ‡Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53703, United States.
authors.forename: Martin T
authors.initials: MT
authors.lastname: Zanni
authors.affiliation: †Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Chen
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010100:Oxygen / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D023181:Antimicrobial Cationic Peptides; D002247:Carbon Isotopes; D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; C440189:ovispirin; D010100:Oxygen; C030345:1,2-dipalmitoylphosphatidylglycerol