title: Ionic Liquid Surfactant Mediated Structural Transitions and Self-Assembly of Bovine Serum Albumin in Aqueous Media: Effect of Functionalization of Ionic Liquid Surfactants.
abstract: The self-assembly of globular protein bovine serum albumin (BSA) has been investigated in aqueous solutions of ionic liquid surfactants (ILSs), 1-dodecyl-3-methyl imidazolium chloride, [C12mim][Cl], and its amide, [C12Amim][Cl], and ester, [C12Emim][Cl], functionalized counterparts. Dynamic light scattering (DLS) has provided insights into the alterations in hydrodynamic radii (D(h)) of BSA as a function of concentration of ILSs establishing the presence of different types of BSA-ILS complexes in different concentration regimes of ILSs. Isothermal titration calorimetry (ITC) has been exploited to quantify the ILSs interacting with BSA in dilute concentration regime of ILSs. The zeta-potential measurements shed light on changes in the charged state of BSA. The morphology of various self-assembled structures of BSA in different concentration regimes of ILSs have been explored using confocal laser scanning microscopy (CLSM) and scanning electron microscopy. The structural variations in ILSs have been found to produce remarkable effect on the nature and morphology of self-assembled structures of BSA. The presence of nonfunctionalized [C12mim][Cl] IL at all investigated concentrations has led to the formation of unordered large self-assembled structures of BSA. On the other hand, in specific concentration regimes, ordered self-assembled structures such as long rods and right-handedly twisted helical amyloid fibers have been observed in the presence of functionalized [C12Amim][Cl] and [C12Emim][Cl] ILSs, respectively. The nature of the formed helical fibers as amyloid ones has been confirmed using FTIR spectroscopy. Steady-state fluorescence and circular dichroism (CD) spectroscopy have provided insights into folding and unfolding of BSA as fashioned by interactions with ILSs in different concentration regimes supporting the observations made from other studies.
authors.affiliation: Department of Chemistry, UGC-Centre for Advance Studies-I, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Gurbir
authors.initials: G
authors.lastname: Singh
authors.affiliation: Department of Chemistry, UGC-Centre for Advance Studies-I, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Tejwant Singh
authors.initials: TS
authors.lastname: Kang
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D055503:Protein Multimerization / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D052578:Ionic Liquids; D013501:Surface-Active Agents; D014867:Water; D012710:Serum Albumin, Bovine