title: In vivo mouse fluorescence imaging for folate-targeted delivery and release kinetics.
abstract: Many cancer cells over-express folate receptors, and this provides an opportunity for both folate-targeted fluorescence imaging and the development of targeted anti-cancer drugs. We present an optical imaging modality that allows for the monitoring and evaluation of drug delivery and release through disulfide bond reduction inside a tumor in vivo for the first time. A near-infrared folate-targeting fluorophore pair was synthesized and used to image a xenograft tumor grown from KB cells in a live mouse. The in vivo results are shown to be in agreement with previous in vitro studies, confirming the validity and feasibility of our method as an effective tool for preclinical studies in drug development.
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Esther H R
authors.initials: EH
authors.lastname: Tsai
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Brian Z
authors.initials: BZ
authors.lastname: Bentz
authors.affiliation: Department of Chemistry, Indian Institute of Technology Indore, DAVV-IET Campus, Indore 452017, Madhya Pradesh, India ; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Chelvam
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Gaind
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Webb
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Philip S
authors.initials: PS
authors.lastname: Low
keywords: (100.3190) Inverse problems; (170.3880) Medical and biological imaging