title: Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content.
abstract: Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome.
authors.affiliation: Systems Biology Laboratory, Centre for Molecular Biology and Genetic Engineering, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Vicentini
authors.affiliation: Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bottcher
authors.affiliation: Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil; Sugarcane Center, Agronomic Institute of Campinas, Ribeirão Preto, SP, Brazil.
authors.forename: Michael Dos Santos
authors.initials: Mdos S
authors.lastname: Brito
authors.affiliation: Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Adriana Brombini
authors.initials: AB
authors.lastname: Dos Santos
authors.affiliation: Sugarcane Center, Agronomic Institute of Campinas, Ribeirão Preto, SP, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Creste
authors.affiliation: Sugarcane Center, Agronomic Institute of Campinas, Ribeirão Preto, SP, Brazil.
authors.forename: Marcos Guimarães de Andrade
authors.initials: MG
authors.lastname: Landell
authors.affiliation: Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Cesarino
authors.affiliation: Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Mazzafera
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D008031:Lignin / Q000032:analysis* / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D018547:Plant Stems / Q000378:metabolism; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D031786:Saccharum / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D008031:Lignin