title: High nuclear expression of protein arginine methyltransferase-5 is a potentially useful marker to estimate submucosal invasion in endoscopically resected early colorectal carcinoma.
abstract: Protein arginine methyltransferase-5 (PRMT5), a major type II arginine methyltransferase, is an important epigenetic modifier with oncogene-like properties because of its ability to repress the expression of tumor suppressor genes. We determined the correlations between PRMT5 expression or its cellular localization and malignant progression, with special reference to invasiveness, in colorectal adenomas and early colorectal carcinomas (CRCs). We performed immunohistochemical detection of PRMT5 in 108 endoscopically resected tissue samples (6 adenomas with low-grade dysplasia, 34 adenomas with high-grade dysplasia, 30 intramucosal carcinomas, and 38 submucosal invasive carcinomas). Early CRC (55 of 68, 80.9%) showed more frequent nuclear expression of PRMT5 than adenoma (15 of 40, 37.5%) (P < 0.001). Furthermore, high (strong staining in ≥ 50% of nuclei) nuclear expression of PRMT5 was more common in submucosal invasive carcinoma (21 of 38, 55.3%) than in intramucosal carcinoma (9 of 30, 30.0%) (P = 0.037). In conclusion, our data suggests that high nuclear expression of PRMT5 is a potentially useful marker for estimating submucosal invasion of early CRC in endoscopically resected specimens.
authors.affiliation: Department of Pathology, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Min Gyoung
authors.initials: MG
authors.lastname: Pak
authors.affiliation: Department of Pathology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Korea.
authors.forename: Hyoun Wook
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Pathology, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Mee Sook
authors.initials: MS
authors.lastname: Roh
mesh_terms: D000236:Adenoma / Q000201:enzymology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000473:pathology*; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C077013:PRMT2 protein, human; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases
keywords: adenoma; early colorectal carcinoma; endoscopic treatment; protein arginine methyltransferase-5; submucosal invasion