title: Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton.
abstract: The zebrafish fin is widely used as a model for skeleton regeneration. For years, the nature of the fin skeleton has been controversial as its extracellular matrix shows hybrid characteristics of both bone and cartilage. The presence of co-orthologs genes also increases the complexity of these tissues. In this article, we have identified and described the expression of fibrillar collagens in zebrafish fin skeleton. We found that genes coding for types I, II, V, XI and XXVII collagens are duplicated, showing in several cases, different expression domains. We also identified specific genomic features, such as the presence of type XXIV collagen and the absence of type III collagen in the zebrafish genome. Our study showed that actinotrichia-forming cells and osteoblasts synthesize a wide variety of these fibrillar collagens during fin regeneration. An intertrichial domain expressing most of the collagens was located in the transition between the mesenchyme condensations of actinotrichia and lepidotrichia and may determine an important niche associated with fin skeleton morphogenesis. We also confirmed the hybrid nature of the fin exoskeleton and provided a complete description of those fibrillar collagens expressed during the formation of the fin skeleton.
authors.affiliation: Department of Cell Biology, Genetics and Physiology, Faculty of Science, IBIMA, University of Malaga, 29071, Málaga, Spain; Department of Orthopedic Surgery, University of California, Los Angeles, Los Angeles, CA, 90095, USA; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 29071, Málaga, Spain. Electronic address: iduranjimenez@mednet.ucla.edu.
authors.forename: I
authors.initials: I
authors.lastname: Duran
authors.affiliation: Molecular and Computational Biology Department, USC, Los Angeles, CA, 90089, USA.
authors.forename: F
authors.initials: F
authors.lastname: Csukasi
authors.affiliation: Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Taylor
authors.affiliation: Department of Orthopedic Surgery, University of California, Los Angeles, Los Angeles, CA, 90095, USA; Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
authors.forename: D
authors.initials: D
authors.lastname: Krakow
authors.affiliation: Department of Cell Biology, Genetics and Physiology, Faculty of Science, IBIMA, University of Malaga, 29071, Málaga, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 29071, Málaga, Spain; Andalusian Center for Biotechnology and Nanomedicine (BIONAND), 29590, Málaga, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Becerra
authors.affiliation: Department of Horticulture, Virginia Tech, Blacksburg, VA, 24060, USA.
authors.forename: A
authors.initials: A
authors.lastname: Bombarely
authors.affiliation: Department of Cell Biology, Genetics and Physiology, Faculty of Science, IBIMA, University of Malaga, 29071, Málaga, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 29071, Málaga, Spain. Electronic address: beffa@uma.es.
authors.forename: M
authors.initials: M
authors.lastname: Marí-Beffa
mesh_terms: D058500:Animal Fins / Q000502:physiology*; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism / Q000502:physiology; D002356:Cartilage / Q000378:metabolism / Q000502:physiology; D003094:Collagen / Q000235:genetics; D024022:Fibrillar Collagens / Q000235:genetics* / Q000378:metabolism; D020131:Genes, Duplicate; D008648:Mesoderm / Q000378:metabolism; D009024:Morphogenesis / Q000235:genetics; D010802:Phylogeny; D012038:Regeneration / Q000235:genetics* / Q000502:physiology; D020539:Sequence Analysis, Protein; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D024022:Fibrillar Collagens; D029961:Zebrafish Proteins; C467802:collagen type XXIV; D003094:Collagen
keywords: Actinotrichia; Fibrillar collagens; Intertrichial; Lepidotrichia