title: Simultaneous determination of scopolamine, hyoscyamine and anisodamine in in vitro growth media of selected Solanaceae hairy roots by CE method.
abstract: An electrophoretic method for fast separation of three tropane alkaloids (hyoscyamine, anisodamine and scopolamine) was presented. The substances were complete resolved in less than one minute due to utilization of relatively short capillary (20.2cm effective length) and high voltage (25kV). Detector probing frequency was found among the parameters that significantly affected the detection sensitivity. The performed experiments showed insufficient available probing frequency of used commercial spectrophotometric detector according to capillary electrophoresis (CE) separation potential. Under the optimized conditions the background electrolyte (BGE) was composed of 20mM Tris, 6mM HCl and 20mM NaCl (pH 8.50). All analyses were carried out in fused silica capillaries of 50μm (inner diameter) and 31.2cm (total capillary length). Samples were injected hydrodynamically (5s; 3.45kPa) without any sample preparation step and separation was performed at 25kV. The elaborated method was applied in plant cultures growth media analysis after incubation with hairy roots of selected Solanaceae species. The performed experiments proved the usefulness of CE in quality control of biotechnological processes.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 107 Hallera Street, 80-416 Gdańsk, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Dziomba
authors.affiliation: Institute of Organic Synthesis, Department of Organic Chemistry, Faculty of Chemistry, University of Gdańsk, 63 Wita Stwosza Street, 80-952 Gdańsk, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Łepek
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, 107 Hallera Street, 80-416 Gdańsk, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Jaremicz
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, 107 Hallera Street, 80-416 Gdańsk, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Łuczkiewicz
authors.affiliation: Institute of Organic Synthesis, Department of Organic Chemistry, Faculty of Chemistry, University of Gdańsk, 63 Wita Stwosza Street, 80-952 Gdańsk, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Prahl
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 107 Hallera Street, 80-416 Gdańsk, Poland. Electronic address: piotr.kowalski@gumed.edu.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kowalski
mesh_terms: D003470:Culture Media; D019075:Electrophoresis, Capillary / Q000379:methods*; D064692:Hyoscyamine / Q000032:analysis*; D066298:In Vitro Techniques; D018517:Plant Roots / Q000737:chemistry*; D012601:Scopolamine / Q000032:analysis*; D019657:Solanaceae / Q000737:chemistry*; D012991:Solanaceous Alkaloids / Q000032:analysis*
chemical_list: D003470:Culture Media; D012991:Solanaceous Alkaloids; C003922:anisodamine; D012601:Scopolamine; D064692:Hyoscyamine
keywords: Anisodamine; Biotechnology; Capillary zone electrophoresis; Hyoscyamine; Scopolamine; Tropane alkaloids