title: Graphene-Based Nanomaterials as Efficient Peroxidase Mimetic Catalysts for Biosensing Applications: An Overview.
abstract: "Artificial enzymes", a term coined by Breslow for enzyme mimics is an exciting and promising branch of biomimetic chemistry aiming to imitate the general and essential principles of natural enzymes using a variety of alternative materials including heterogeneous catalysts. Peroxidase enzymes represent a large family of oxidoreductases that typically catalyze biological reactions with high substrate affinity and specificity under relatively mild conditions and thus offer a wide range of practical applications in many areas of science. The increasing understanding of general principles as well as intrinsic drawbacks such as low operational stability, high cost, difficulty in purification and storage, and sensitivity of catalytic activity towards atmospheric conditions of peroxidases has triggered a dynamic field in nanotechnology, biochemical, and material science that aims at joining the better of three worlds by combining the concept adapted from nature with the processability of catalytically active graphene-based nanomaterials (G-NMs) as excellent peroxidase mimetic catalysts. This comprehensive review discusses an up-to-date synthesis, kinetics, mechanisms, and biosensing applications of a variety of G-NMs that have been explored as promising catalysts to mimic natural peroxidases.
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan. bhaskargarg111@gmail.com.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Garg
authors.affiliation: Department of Chemistry, Government Degree College, Champawat 262523, Uttarakhand, India. tanuja.bisht16@gmail.com.
authors.forename: Tanuja
authors.initials: T
authors.lastname: Bisht
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan. ycling@mx.nthu.edu.tw.
authors.forename: Yong-Chien
authors.initials: YC
authors.lastname: Ling
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D006108:Graphite / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*
chemical_list: D006108:Graphite; D009195:Peroxidase
keywords: biosensing and diagnostics; enzyme mimetics; graphene; graphene oxide; graphene-based nanomaterials; heterogeneous catalysts; hydrogen peroxide; peroxidases; tetramethylbenzidine