title: Effects of antipsychotic drugs on the expression of synapse-associated proteins in the frontal cortex of rats subjected to immobilization stress.
abstract: The present study examined the effects of antipsychotic drugs on the expression of synapse-associated proteins in the frontal cortex of rats with and without immobilization stress. Rats were subjected to immobilization stress 6h/day for 3 weeks. The effects of atypical antipsychotic drugs, olanzapine and aripiprazole, on expression of serine(9)-phosphorylated GSK-3β, β-catenin, BDNF, PSD-95, and synaptophysin were determined by Western blotting. A typical antipsychotic drug, haloperidol, was used for comparison. Immobilization stress significantly decreased the expression of these proteins in the frontal cortex. Chronic administration of olanzapine and aripiprazole significantly attenuated the immobilization stress-induced decrease in the levels of these proteins, whereas haloperidol had no such effect. Additionally, olanzapine and aripiprazole significantly increased levels of phosphorylated GSK-3β under normal conditions without stress, and aripiprazole also increased BDNF levels under this condition. These results indicate that olanzapine and aripiprazole, and, haloperidol, differentially regulate the levels of synapse-associated proteins in the rat frontal cortex. These findings may contribute to explain the neurobiological basis of how olanzapine and aripiprazole up-regulated synapse-associated proteins.
authors.affiliation: Paik Institute for Clinical Research, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, Republic of Korea.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Seo
authors.affiliation: Paik Institute for Clinical Research, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, Republic of Korea.
authors.forename: Chan Hong
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Paik Institute for Clinical Research, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, Republic of Korea.
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Cho
authors.affiliation: Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea.
authors.forename: Young Sun
authors.initials: YS
authors.lastname: You
authors.affiliation: Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea.
authors.forename: Bong Ju
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea; Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea.
authors.forename: Jung Goo
authors.initials: JG
authors.lastname: Lee
authors.affiliation: Paik Institute for Clinical Research, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, Republic of Korea; Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea. Electronic address: swpark@inje.ac.kr.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Park
authors.affiliation: Paik Institute for Clinical Research, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, Republic of Korea; Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea; Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea. Electronic address: neuro109@hanmail.net.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D000068180:Aripiprazole / Q000494:pharmacology*; D001569:Benzodiazepines / Q000494:pharmacology*; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000187:drug effects / Q000378:metabolism; D000076122:Disks Large Homolog 4 Protein; D005625:Frontal Lobe / Q000187:drug effects* / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000187:drug effects; D000071679:Glycogen Synthase Kinase 3 beta; D006220:Haloperidol / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D000077152:Olanzapine; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological; D013569:Synapses; D016708:Synaptophysin / Q000187:drug effects / Q000378:metabolism; D051176:beta Catenin / Q000187:drug effects / Q000378:metabolism
chemical_list: D014150:Antipsychotic Agents; D019208:Brain-Derived Neurotrophic Factor; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D016708:Synaptophysin; D051176:beta Catenin; D001569:Benzodiazepines; D000068180:Aripiprazole; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3; D006220:Haloperidol; D000077152:Olanzapine
keywords: Atypical antipsychotic drugs; Immobilization stress; Synapse-associated proteins; Synaptic plasticity; Typical antipsychotic drugs