title: The role of high cholesterol-high fructose diet on coronary arteriosclerosis.
abstract: The effect of fructose in conjunction with high cholesterol diet in the development of atherosclerotic lesions in coronary arteries is not well established. Microswine were fed high cholesterol (HC) or a high cholesterol-high fructose (HCHF) diet containing 18-20% calories from fructose. All swine had high levels of serum cholesterol and non-HDL, thickened intima and accumulation of collagen in the coronaries. Swine fed with HC diet had less stenosis in coronary arteries, lower serum levels of non-HDL, triglycerides, cholesterol, and blood glucose than HCHF group. Coronary lesions in the HC swine were not as progressed as in HCHF and showed low LDL-expressed lipid-laden foam cells. The M1/M2 macrophage phenotype in the HCHF swine differed with the progression of atherosclerosis, with higher density of M1-phenotype in HCHF swine. There was high expression of CCR7 (M1-phenotype) in more advanced lesions in the fibrous cap-like areas, whereas M2-macrophages were abundant in the foam-cell cores. These findings suggest that the addition of a fructose to high cholesterol diet accelerates atherosclerotic lesions in coronary arteries with an increase in M1-macrophages and the propensity to develop features of metabolic syndrome.
authors.affiliation: Center for Clinical and Translational Science, Creighton University School of Medicine, Omaha, NE, USA.
authors.forename: Vicki J
authors.initials: VJ
authors.lastname: Swier
authors.affiliation: Center for Clinical and Translational Science, Creighton University School of Medicine, Omaha, NE, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.affiliation: Center for Clinical and Translational Science, Creighton University School of Medicine, Omaha, NE, USA.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Radwan
authors.affiliation: Center for Clinical and Translational Science, Creighton University School of Medicine, Omaha, NE, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Hunter
authors.affiliation: Center for Clinical and Translational Science, Creighton University School of Medicine, Omaha, NE, USA. dkagr@creighton.edu.
authors.forename: Devendra K
authors.initials: DK
authors.lastname: Agrawal
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000503:physiopathology; D002791:Cholesterol, Dietary; D003324:Coronary Artery Disease / Q000503:physiopathology*; D003331:Coronary Vessels / Q000473:pathology; D004032:Diet; D004036:Diet, Atherogenic; D018450:Disease Progression; D005260:Female; D005632:Fructose / Q000008:administration & dosage / Q000009:adverse effects*; D006937:Hypercholesterolemia / Q000503:physiopathology; D008077:Lipoproteins, LDL / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D024821:Metabolic Syndrome / Q000503:physiopathology; D010641:Phenotype; D054400:Receptors, CCR7 / Q000378:metabolism; D012307:Risk Factors; D013552:Swine; D013556:Swine, Miniature
chemical_list: D002791:Cholesterol, Dietary; D008077:Lipoproteins, LDL; D054400:Receptors, CCR7; D005632:Fructose