title: One-pot β-cyclodextrin-assisted extraction of active ingredients from Xue-Zhi-Ning basing its encapsulated ability.
abstract: Xue-Zhi-Ning (XZN) is a traditional Chinese medicine formula, containing active ingredients with poor solubility in water, which has been demonstrated to be helpful for patients with hyperlipidemia. One-pot β-cyclodextrin (β-CD)-assisted extraction of active ingredients from XZN has been carried out to develop an efficient and eco-friendly extraction process. Five active compounds--rubrofusarin gentiobioside, 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside, emodin, nuciferine and quercetin--were identified by UPLC/DAD/MS and used as indexes to evaluate the process optimized by an orthogonal test. The results showed that addition of β-CD significantly enhanced the extraction ratios of all five components. The enhancement of extraction ratios was positively correlated with the apparent formation constants between β-CD and the compounds. The study also showed that the stabilities and dissolution rates of the active ingredients were improved in the presence of β-CD. This one-pot β-cyclodextrin-assisted extraction has the potential to be applied in pharmaceutical preparations directly.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Liu
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Yue-Fei
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Deng
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Ming-Lei
authors.initials: ML
authors.lastname: Cui
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China. Electronic address: xiaoliang_ren@sina.com.
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Ren
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Ai-Di
authors.initials: AD
authors.lastname: Qi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D012995:Solubility; D014867:Water; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D047392:beta-Cyclodextrins; D014867:Water; C031215:betadex
keywords: 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-d-glucoside (PubChem CID: 91632914); Apparent formation constant; Dissolution rate; Emodin (PubChem CID: 3220); Extraction process; Nuciferine (PubChem CID: 10146); Quercetin (PubChem CID: 5280343); Rubrofusarin gentiobioside (PubChem CID: 503733); Stability; Xue–Zhi–Ning; β-Cyclodextrin