title: DNA break-induced sumoylation is enabled by collaboration between a SUMO ligase and the ssDNA-binding complex RPA.
abstract: Upon genome damage, large-scale protein sumoylation occurs from yeast to humans to promote DNA repair. Currently, the underlying mechanism is largely unknown. Here we show that, upon DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance. Both RPA and nuclease-generated ssDNA promote Siz2-mediated sumoylation. Mechanistically, the conserved Siz2 interaction with RPA enables Siz2 localization to damage sites. These findings provide a molecular basis for recruiting SUMO ligases to the vicinity of their substrates to induce sumoylation upon DNA damage.
authors.affiliation: Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA.
authors.forename: Inn
authors.initials: I
authors.lastname: Chung
authors.affiliation: Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhao
mesh_terms: D001761:Bleomycin / Q000494:pharmacology; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects; D004260:DNA Repair / Q000235:genetics / Q000502:physiology*; D009153:Mutagens / Q000494:pharmacology; D021381:Protein Transport; D051716:Replication Protein A / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D058207:Sumoylation
chemical_list: D009153:Mutagens; D051716:Replication Protein A; D029701:Saccharomyces cerevisiae Proteins; C436836:Siz2 protein, S cerevisiae; D001761:Bleomycin; C105427:Zeocin
keywords: DNA break resection; DNA damage response; homologous recombination; protein sumoylation