title: High-resolution crystal structure of cAMP-dependent protein kinase from Cricetulus griseus.
abstract: Protein kinases (PKs) are dynamic regulators of numerous cellular processes. Their phosphorylation activity is determined by the conserved kinase core structure, which is maintained by the interaction and dynamics with associated domains or interacting proteins. The prototype enzyme for investigations to understand the activity and regulation of PKs is the catalytic subunit of cAMP-dependent protein kinase (PKAc). Major effects of functional regulation and ligand binding are driven by only minor structural modulations in protein-protein interactions. In order to resolve such minor structural differences, very high resolution structures are required. Here, the high-resolution X-ray structure of PKAc from Cricetulus griseus is reported.
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Denis
authors.initials: D
authors.lastname: Kudlinzki
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Verena L
authors.initials: VL
authors.lastname: Linhard
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Krishna
authors.initials: K
authors.lastname: Saxena
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Sridhar
authors.initials: S
authors.lastname: Sreeramulu
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Santosh
authors.initials: S
authors.lastname: Gande
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schieborr
authors.affiliation: R&D, Sanofi, Industriepark Hoechst Building G838, 65926 Frankfurt, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Dreyer
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Schwalbe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D003412:Cricetulus; D003460:Crystallization; D018360:Crystallography, X-Ray; D000242:Cyclic AMP / Q000737:chemistry*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D011993:Recombinant Fusion Proteins; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: ATP binding; NTP binding; PKA; cAMP; kinase; nucleotide binding; serine/threonine protein kinase; transferase