title: Seco-ursane-type Triterpenoids from Salvia urmiensis with Apoptosis-inducing Activity.
abstract: Fractionation of an acetone extract of the aerial parts of Salvia urmiensis led to the isolation of a new (1) and a known (2) E-seco-ursane-type triterpenoid, together with four other known compounds. Their structures were established by 1D and 2D nuclear magnetic resonance as well as high-resolution electrospray ionization mass spectrometry. The effect of compounds 1 and 2 on cell viability of HeLa and HepG2 cells was investigated with the MTT assay. We also report the mechanism of action of compound 2 as a potential anticancer agent in HeLa cells. Bcl-2, Bax, and caspases signaling pathway expression in HeLa cells was analyzed. HeLa cells treated with compound 2 were assayed for the cleavage of poly-(ADP-ribose)-polymerase and DNA fragmentation resulting in nuclear shrinkage. Taken together, these results suggest that treatment of HeLa cells with compound 2 can induce apoptosis by regulating Bcl-2 family members and by suppressing caspase cascade activation.
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Mahdi Moridi
authors.initials: MM
authors.lastname: Farimani
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Abbas-Mohammadi
authors.affiliation: Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Esmaeili
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Salehi
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Samad
authors.initials: S
authors.lastname: Nejad-Ebrahimi
authors.affiliation: Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Sonboli
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D027544:Salvia / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D053978:Pentacyclic Triterpenes
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D047310:Apigenin; D020169:Caspases; D009828:Oleanolic Acid; D019253:Proto-Oncogene Proteins c-bcl-2; D012855:Sitosterols; D014315:Triterpenes; D051028:bcl-2-Associated X Protein; C494826:BAX protein, human; C000606873:ursane; D053978:Pentacyclic Triterpenes; C011015:lyoniside