title: An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis.
abstract: The evolutionary conserved NSL complex is a prominent epigenetic regulator controlling expression of thousands of genes. Here we uncover a novel function of the NSL complex members in mitosis. As the cell enters mitosis, KANSL1 and KANSL3 undergo a marked relocalisation from the chromatin to the mitotic spindle. By stabilizing microtubule minus ends in a RanGTP-dependent manner, they are essential for spindle assembly and chromosome segregation. Moreover, we identify KANSL3 as a microtubule minus-end-binding protein, revealing a new class of mitosis-specific microtubule minus-end regulators. By adopting distinct functions in interphase and mitosis, KANSL proteins provide a link to coordinate the tasks of faithful expression and inheritance of the genome during different phases of the cell cycle.
authors.affiliation: 1] Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain [2] Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Meunier
authors.affiliation: 1] Max Planck Institute of Immunobiology and Epigenetics, Department of Chromatin Regulation, 79108 Freiburg im Breisgau, Germany [2] Faculty of Biology, University of Freiburg, 79104 Freiburg im Breisgau, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Shvedunova
authors.affiliation: 1] Max Planck Institute of Immunobiology and Epigenetics, Department of Chromatin Regulation, 79108 Freiburg im Breisgau, Germany [2] Faculty of Biology, University of Freiburg, 79104 Freiburg im Breisgau, Germany.
authors.forename: Nhuong
authors.initials: N
authors.lastname: Van Nguyen
authors.affiliation: 1] Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain [2] Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Leonor
authors.initials: L
authors.lastname: Avila
authors.affiliation: 1] Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003 Barcelona, Spain [2] Universitat Pompeu Fabra (UPF), Dr Aiguader 88, 08003 Barcelona, Spain [3] Institució Catalana de Recerca i Estudis Avançats (ICREA), Passeig Lluís Companys 23, 08010 Barcelona, Spain.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vernos
authors.affiliation: Max Planck Institute of Immunobiology and Epigenetics, Department of Chromatin Regulation, 79108 Freiburg im Breisgau, Germany.
authors.forename: Asifa
authors.initials: A
authors.lastname: Akhtar
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D004331:Drosophila melanogaster; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D009687:Nuclear Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D014982:Xenopus laevis; D020931:ran GTP-Binding Protein / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C000607306:KANSL3 protein, human; D008869:Microtubule-Associated Proteins; C574033:NSL1 protein, human; D009687:Nuclear Proteins; C122167:TPX2 protein, human; D020931:ran GTP-Binding Protein