title: Genome expression of Thermococcus barophilus and Thermococcus kodakarensis in response to different hydrostatic pressure conditions.
abstract: Transcriptomes were analyzed for two related hyperthermophilic archaeal species, the piezophilic Thermococcus barophilus strain MP and piezosensitive Thermococcus kodakarensis strain KOD1 subjected to high hydrostatic pressures. A total of 378 genes were differentially expressed in T. barophilus cells grown at 0.1, 40 and 70 MPa, whereas 141 genes were differentially regulated in T. kodakarensis cells grown at 0.1 and 25 MPa. In T. barophilus cells grown under stress conditions (0.1 and 70 MPa), 178 upregulated genes were distributed among three clusters of orthologous groups (COG): energy production and conversion (C), inorganic ion transport and metabolism (P) and carbohydrate transport and metabolism (G), whereas 156 downregulated genes were distributed among: amino acid transport and metabolism (E), replication, recombination and repair (L) and nucleotide transport and metabolism (F). The expression of 141 genes was regulated in T. kodakarensis cells grown under stress conditions (25 MPa); 71 downregulated genes belong to three COG: energy production and conversion (C), amino acid transport and metabolism (E) and transcription (K), whereas 70 upregulated genes are associated with replication, recombination and repair (L), coenzyme transport (H) and defense mechanisms (V).
authors.affiliation: Matís ohf., Food Safety, Environment and Genetics, Vinlandsleið 12, 113 Reykjavik, Iceland; Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; CNRS, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Technopôle Brest-Iroise, BP70, 29 280 Plouzané, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Vannier
authors.affiliation: Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; CNRS, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Technopôle Brest-Iroise, BP70, 29 280 Plouzané, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Michoud
authors.affiliation: Laboratoire de Géologie de Lyon, UMR 5276 CNRS, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon, Lyon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Oger
authors.affiliation: Matís ohf., Food Safety, Environment and Genetics, Vinlandsleið 12, 113 Reykjavik, Iceland; Agricultural University of Iceland, Hvanneyri, 311 Borgarnes, Iceland. Electronic address: viggo.th.marteinsson@matis.is.
authors.forename: Viggó Þór
authors.initials: VÞ
authors.lastname: Marteinsson
authors.affiliation: Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; CNRS, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France; Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Technopôle Brest-Iroise, BP70, 29 280 Plouzané, France. Electronic address: mohamed.jebbar@univ-brest.fr.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jebbar
mesh_terms: D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D020869:Gene Expression Profiling; D020745:Genome, Archaeal; D006874:Hydrostatic Pressure; D013312:Stress, Physiological / Q000235:genetics*; D019712:Thermococcus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000596:Amino Acids
keywords: Archaea; HHP responsive genes; Hyperthermophilic; Piezophily; Transcriptomic