title: Study on Antitumor Platinum(II) Complexes of Chiral Diamines with Dicyclic Species as Steric Hindrance.
abstract: A series of platinum(II) complexes, characteristic of chiral trans-bicyclo[2.2.2]octane-7,8-diamine as ligand possessing dicyclic steric hindrance, were designed and synthesized. Biological evaluation showed that almost all complexes had cytotoxic activity against the tested cancer cell lines, among which most of chiral (R,R)-enantiomeres had stronger cytotoxicity than their (S,S)-counterparts, and 2a, [trans-bicyclo[2.2.2]octane-7R,8R-diamine](oxalato-O,O')platinum(II), is the most effective agent. Significantly, its counterpart, 2b, was much more sensitive to cisplatin resistant SGC7901/CDDP cancer cell line at a higher degree than 2a. Docking study and agarose gel electrophoresis revealed that the interaction of 2a with DNA was similar to that of oxaliplatin. Western blot analysis demonstrated that 2a could induce a better effect than cisplatin on a mitochondrial-dependent apoptosis pathway. Kinetic study indicated that the dicyclic ligand can accelerate the reaction rate of the complex.
authors.affiliation: Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University , Campus of Jiulong Lake in Jiangning District, Nanjing 211189, China.|Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University , Nanjing 211189, China.
authors.forename: Fengfan
authors.initials: F
authors.lastname: Liu
authors.affiliation: Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University , Campus of Jiulong Lake in Jiangning District, Nanjing 211189, China.|Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University , Nanjing 211189, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gou
authors.affiliation: Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University , Campus of Jiulong Lake in Jiangning District, Nanjing 211189, China.|Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University , Nanjing 211189, China.
authors.forename: Feihong
authors.initials: F
authors.lastname: Chen
authors.affiliation: Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University , Campus of Jiulong Lake in Jiangning District, Nanjing 211189, China.|Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University , Nanjing 211189, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.affiliation: Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University , Campus of Jiulong Lake in Jiangning District, Nanjing 211189, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000187:drug effects; D003959:Diamines / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008024:Ligands; D008968:Molecular Conformation; D009944:Organoplatinum Compounds / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology*; D000077150:Oxaliplatin; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D003959:Diamines; D008024:Ligands; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin