title: Withaferin A attenuates lipopolysaccharide-induced acute lung injury in neonatal rats.
abstract: Withaferin A (WFA) is an active compound from Withania somnifera and has been reported to exhibit a variety of pharmacological activities such as anti&mdash;inflammatory, immunomodulatory and anti&mdash;tumor properties. In the present study, we investigated the potential protective role of WFA on acute lung injury in neonatal rats induced by lipopolysaccharide (LPS). We found that WFA significantly attenuated the pathological changes of lungs induced by LPS injection. Administration with WFA obviously decreased pulmonary neutrophil infiltration accompanied with decreased MPO concentrations. WFA also reduced the expression of pro&mdash;inflammatory cytokines including MIP&mdash;2, TNF&mdash;&#945;, IL&mdash;1&#946; and IL&mdash;6. Meanwhile, the expression levels of anti&mdash;inflammatory mediators such as TGF&mdash;&#946;1 and IL&mdash;10 were significantly increased following WFA administration. Moreover, WFA protected LPS&mdash;treated rats from oxidative damage via up&mdash;regulation of TBARS and H2O2 concentrations and down&mdash;regulation of ROS contents. Taken together, the present study demonstrated that WFA administration attenuated LPS&mdash;induced lung injury through inhibition of inflammatory responses and oxidative stress.
authors.affiliation: Woman and Child Hospital of Hubei Province Department of Pediatrics Wuhan China.
authors.forename: S
authors.initials: S
authors.lastname: Gao
authors.affiliation: Woman and Child Hospital of Hubei Province Department of Pediatrics Wuhan China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Woman and Child Hospital of Hubei Province Department of Pediatrics Wuhan China.
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Zhou
authors.affiliation: Woman and Child Hospital of Hubei Province Department of Pediatrics Wuhan China Youjb5813&commat;163.com.
authors.forename: J-B
authors.initials: JB
authors.lastname: You
authors.affiliation: Woman and Child Hospital of Hubei Province Department of Pediatrics Wuhan China.
authors.forename: D-N
authors.initials: DN
authors.lastname: Tu
authors.affiliation: Xinhua Hospital of Hubei Province Department of Pathology Wuhan China.
authors.forename: G
authors.initials: G
authors.lastname: Xia
authors.affiliation: Affiliated Hospital of Guiyang Medical College Department of Biliary&mdash;Hepatic Surgery Guiyang China.
authors.forename: J-X
authors.initials: JX
authors.lastname: Jiang
authors.affiliation: Woman and Child Hospital of Hubei Province Department of Pediatrics Wuhan China.
authors.forename: C
authors.initials: C
authors.lastname: Xin
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000517:prevention & control*; D000818:Animals; D000831:Animals, Newborn; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006861:Hydrogen Peroxide / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D032341:Withania / Q000737:chemistry / Q000378:metabolism; D054358:Withanolides / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D054358:Withanolides; D006861:Hydrogen Peroxide; C009684:withaferin A