title: Monocytes play different roles in stimulating T cells in obese diabetic individuals.
abstract: Type 2 diabetes (T2D) is a chronic metabolic disorder, which was also found to involve a series of inflammatory disorders, including accumulation of macrophages and T cells in the adipose tissue, increased proinflammatory cytokine production, shifting of macrophage composition toward M1-type, and skewing of peripheral blood T cells toward IL-17 productions. However, these studies were primarily conducted in obese mouse models and/or human subjects with higher BMI, and may not reflect the role of the immune system in non-obese T2D pathogenesis. Here, we examined T cell and monocyte cytokine expression and function in both non-obese and obese T2D patients. We found that IFN-g production by circulating T cells were increased in both non-obese and obese T2D subjects, while IL-17 is only upregulated in obese T2D subjects. Also, circulating monocytes from obese T2D subjects had significantly higher IL-6 production than their counterparts in non-obese T2D subjects. Moreover, monocytes from non-obese T2D subjects could support IFN-g but not IL-17 production in vitro, while that from obese T2D subjects supported both IFN-g and IL-17 production. Together, our results revealed that the role immune system plays in T2D pathogenesis is more complicated than previously thought, and is affected by the person's BMI.
authors.affiliation: Surgical Center of Thyroid Diabetes, General Hospital of Guangzhou Military Command of PLA, Guangzhou, PR China.
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Dai
authors.affiliation: Health Management Center, Guangzhou First People's Hospital, Guangzhou Medical College, Guangzhou, PR China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Zhan
authors.affiliation: Faculty of Medicine, University of Maryland, Baltimore, MD, USA.
authors.forename: Todd A
authors.initials: TA
authors.lastname: Demmy
authors.affiliation: University Hospital, University of British Columbia, BC, Canada.
authors.forename: Fred B
authors.initials: FB
authors.lastname: Poordad
authors.affiliation: University Hospital, University of British Columbia, BC, Canada.
authors.forename: Paulette L
authors.initials: PL
authors.lastname: Fauceglia
authors.affiliation: Medical Lab, General Hospital of Guangzhou Military Command of PLA, Guangzhou, PR China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Surgical Center of Thyroid Diabetes, General Hospital of Guangzhou Military Command of PLA, Guangzhou, PR China liangpingwu_gz@126.com.
authors.forename: Liangping
authors.initials: L
authors.lastname: Wu
mesh_terms: D000273:Adipose Tissue / Q000276:immunology; D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D009765:Obesity / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D015850:Interleukin-6; D007371:Interferon-gamma
keywords: IL-17; diabetes; monocyte