title: Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1.
abstract: Sodium fluoride (NaF), used as pesticides and for industrial purposes are deposited in the water bodies and therefore affects its biota. Zebrafish exposed to NaF in laboratory condition showed hyperactivity and frequent surfacing activity, somersaulting and vertical swimming pattern as compared to the control group. Reactive oxygen species level was elevated and glutathione level was depleted along with increased malondialdehyde content in the brain. Levels of glutathione-s-transferase (GST), catalase (CAT) and superoxide dismutase were also elevated in the treatment groups. Expression of mRNA of nuclear factor erythroid 2 related factor 2 (Nrf2) and its inhibitor Kelch-like ECH-associated protein 1 (Keap1) during stress condition were observed along with Gst, Cat, NADPH: quinone oxidoreductase 1(Nqo1) and p38. Except Keap1, all other genes exhibited elevated expression. Nrf2/Keap1 proteins had similar expression pattern as their corresponding mRNA. The findings in this study might help to understand the molecular mechanism of fluoride induced neurotoxicity in fish.
authors.affiliation: Molecular Genetics Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati, Santiniketan 731 235, West Bengal, India.
authors.forename: Debdip
authors.initials: D
authors.lastname: Mukhopadhyay
authors.affiliation: Biology Olympiad Cell, Homi Bhabha Centre for Science Education, Tata Institute of Fundamental Research, V. N. Purav Marg, Mankhurd, Anushakti Nagar, Mumbai 400 088, Maharashtra, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Priya
authors.affiliation: Molecular Genetics Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati, Santiniketan 731 235, West Bengal, India. Electronic address: chansuman1@rediffmail.com.
authors.forename: Ansuman
authors.initials: A
authors.lastname: Chattopadhyay
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers / Q000235:genetics; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012969:Sodium Fluoride / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D005819:Genetic Markers; C527640:Keap1a protein, zebrafish; C527641:Keap1b protein, zebrafish; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D029961:Zebrafish Proteins; C495638:nfe2l2a protein, zebrafish; D012969:Sodium Fluoride
keywords: Brain; Neurotoxicity; Nrf2; Reactive oxygen species; Sodium fluoride; Zebrafish