title: Early second-trimester plasma protein profiling using multiplexed isobaric tandem mass tag (TMT) labeling predicts gestational diabetes mellitus.
abstract: OBJECTIVE
  Gestational diabetes mellitus (GDM) is associated with an increased risk of serious complications for mother and child during pregnancy. The main option for diagnosis of GDM is 75 g oral glucose tolerance test (OGTT) at 24-28 gestation weeks, when harms to both mother and child have already potentially occurred. The aim of this study was to investigate new biomarkers for earlier detection and assessment of GDM at early second trimester (16-18 gestation weeks).
  
  
  METHODS
  We systematically used multiplexed isobaric tandem mass tag labeling combined with liquid chromatography mass spectrometry (LC-MS/MS) to screen differentially expressed proteins in plasma collected at 16-18 gestational weeks between pregnant women with and without GDM outcome.
  
  
  RESULTS
  A total of 828 proteins were identified, of which 36 proteins implicated in immune response, inflammation, transport, platelet aggregation, catalyze and defense response were identified as differentially regulated proteins in GDM. To assess the validity of the results, four selected proteins including C-reactive protein, sex hormone-binding globulin, Ficolin 3 and pregnancy-specific beta-1-glycoprotein 4 were selected for subsequent Western blot analysis.
  
  
  CONCLUSIONS
  This is the first comprehensive study that integrates multiple state-of-the-art proteomic technologies to discover the earlier potential plasma biomarkers for GDM.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Liver Transplantation Center of the First Affiliated Hospital to Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, China. Hxy_njmu@163.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, China. jesse_1982@163.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D016640:Diabetes, Gestational / Q000097:blood* / Q000175:diagnosis; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis; D011262:Pregnancy Trimester, Second / Q000097:blood*; D040901:Proteomics; D013347:Subcellular Fractions / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D011257:Pregnancy Proteins
keywords: Gestational diabetes mellitus; LC-MS/MS; Plasma; TMT