title: Heterotrimeric G protein subunits differentially respond to endoplasmic reticulum stress in Arabidopsis.
abstract: Canonical heterotrimeric G proteins in eukaryotes are major components that localize at plasma membrane and transmit extracellular stimuli into the cell. Genome of a seed plant Arabidopsis thaliana encodes at least one Gα (GPA1), one Gβ (AGB1), and 3 Gγ (AGG1, AGG2 and AGG3) subunits. The loss-of-function mutations of G protein subunit(s) cause multiple defects in development as well as biotic and abiotic stress responses. However, it remains elusive how these subunits differentially express these defects. Here, we report that Arabidopsis heterotrimeric G protein subunits differentially respond to the endoplasmic reticulum (ER) stress. An isolated homozygous mutant of AGB1, agb1-3, was more sensitive to the tunicamycin-induced ER stress compared to the wild type and the other loss-of-function mutants of G protein subunits. Moreover, ER stress responsive genes were highly expressed in the agb1-3 plant. Our results indicate that AGB1 positively contributes to ER stress tolerance in Arabidopsis.
authors.affiliation: a Institute of Plant and Microbial Biology, Academia Sinica ; Taipei , Taiwan.|b Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica ; Taipei , Taiwan.|d Graduate Institute of Biotechnology and Department of Life Sciences ; National Chung-Hsing University ; Taichung , Taiwan.
authors.forename: Yueh
authors.initials: Y
authors.lastname: Cho
authors.affiliation: a Institute of Plant and Microbial Biology, Academia Sinica ; Taipei , Taiwan.
authors.forename: Chao-Yuan
authors.initials: CY
authors.lastname: Yu
authors.affiliation: e Muroran Institute of Technology ; Muroran , Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Iwasa
authors.affiliation: a Institute of Plant and Microbial Biology, Academia Sinica ; Taipei , Taiwan.|b Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica ; Taipei , Taiwan.|c Biotechnology Center, National Chung-Hsing University ; Taichung , Taiwan.|e Muroran Institute of Technology ; Muroran , Japan.
authors.forename: Kazue
authors.initials: K
authors.lastname: Kanehara
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D017343:Genes, Plant; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D021122:Protein Subunits / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological; D014415:Tunicamycin
chemical_list: C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044387:GTP-Binding Protein beta Subunits; D021122:Protein Subunits; D014415:Tunicamycin; D020962:Heterotrimeric GTP-Binding Proteins
keywords: AGB1; Arabidopsis; ER quality control; ER stress response; ER stress tolerance; Endoplasmic reticulum; Heterotrimeric G protein