title: Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference.
abstract: Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.
authors.affiliation: Department of Chemistry, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fujino
authors.affiliation: Department of Chemistry, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Kogashi
authors.affiliation: Department of Chemistry, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Koudai
authors.initials: K
authors.lastname: Okada
authors.affiliation: Department of Chemistry, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Martin
authors.initials: M
authors.lastname: Mattarella
authors.affiliation: Department of Chemistry, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Biomolecular Sciences, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yasumoto
authors.affiliation: Department of Biomolecular Sciences, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sogawa
authors.affiliation: Department of Chemistry, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan. isobe@m.tohoku.ac.jp.|JST, ERATO, Isobe Degenerate π-Integration Project and Advanced Institute for Materials Research, Tohoku University, Aoba-ku, Sendai, 980-8577, Japan. isobe@m.tohoku.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Isobe
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009841:Oligonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D009841:Oligonucleotides; D010710:Phosphates; D034741:RNA, Small Interfering; D014230:Triazoles; C054562:yellow fluorescent protein, Bacteria
keywords: RNA; click chemistry; oligonucleotides; siRNA; solid-phase synthesis