title: Modifications of excitatory and inhibitory transmission in rat hippocampal pyramidal neurons by acute lithium treatment.
abstract: The acute effects of high-dose Li(+) treatment on glutamatergic and GABAergic transmissions were studied in the "synaptic bouton" preparation of isolated rat hippocampal pyramidal neurons by using focal electrical stimulation. Both action potential-dependent glutamatergic excitatory and GABAergic inhibitory postsynaptic currents (eEPSC and eIPSC, respectively) were dose-dependently inhibited in the external media containing 30-150 mM Li(+), but the sensitivity for Li(+) was greater tendency for eEPSCs than for eIPSCs. When the effects of Li(+) on glutamate or GABAA receptor-mediated whole-cell responses (IGlu and IGABA) elicited by an exogenous application of glutamate or GABA were examined in the postsynaptic soma membrane of CA3 neurons, Li(+) slightly inhibited both IGlu and IGABA at the 150 mM Li(+) concentration. Present results suggest that acute treatment with high concentrations of Li(+) acts preferentially on presynaptic terminals, and that the Li(+)-induced inhibition may be greater for excitatory than for inhibitory transmission.
authors.affiliation: Research Division for Clinical Pharmacology, Medical Corporation, Juryokai, Kumamoto Kinoh Hospital, 6-8-1, Yamamuro, Kita-ku, Kumamoto 860-8518, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Wakita
authors.affiliation: Research Division for Clinical Pharmacology, Medical Corporation, Juryokai, Kumamoto Kinoh Hospital, 6-8-1, Yamamuro, Kita-ku, Kumamoto 860-8518, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagami
authors.affiliation: Research Division for Clinical Pharmacology, Medical Corporation, Juryokai, Kumamoto Kinoh Hospital, 6-8-1, Yamamuro, Kita-ku, Kumamoto 860-8518, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takase
authors.affiliation: Research Division for Clinical Pharmacology, Medical Corporation, Juryokai, Kumamoto Kinoh Hospital, 6-8-1, Yamamuro, Kita-ku, Kumamoto 860-8518, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Nakanishi
authors.affiliation: Research Division of Neurophysiology, Kitamoto Hospital, 3-7-6, Kawarasone, Koshigaya 343-0821, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kotani
authors.affiliation: Research Division for Clinical Pharmacology, Medical Corporation, Juryokai, Kumamoto Kinoh Hospital, 6-8-1, Yamamuro, Kita-ku, Kumamoto 860-8518, Japan; Research Division of Neurophysiology, Kitamoto Hospital, 3-7-6, Kawarasone, Koshigaya 343-0821, Japan; Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: akaike.n@juryo.or.jp.
authors.forename: Norio
authors.initials: N
authors.lastname: Akaike
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000502:physiology; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002491:Central Nervous System Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D018020:Lithium Compounds / Q000494:pharmacology*; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D002491:Central Nervous System Agents; D018020:Lithium Compounds; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid
keywords: Acute; Hippocampus; LiCl; eEPSC; eIPSC