title: Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir.
abstract: An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucun drinking-water reservoir. Strain ZF31 removed 97% of nitrate nitrogen after 16h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ. Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72°C, and with shaking at 54.15rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10°C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China. Electronic address: huangtinglin@xauat.edu.cn.
authors.forename: Tinglin
authors.initials: T
authors.lastname: Huang
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China; School of Environment and Planning, Liaocheng University, Liaocheng, Shandong 252000, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China.
authors.forename: Haihan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Su
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wen
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D058440:Denitrification; D060766:Drinking Water / Q000382:microbiology; D008956:Models, Chemical; D009566:Nitrates / Q000032:analysis / Q000378:metabolism*; D009573:Nitrites / Q000032:analysis; D009584:Nitrogen / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D060766:Drinking Water; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen
keywords: Aerobic denitrifier; Nitrogen removal; Pseudomonas stutzeri; Reservoir; Response surface methodology