title: Expression of adenosine receptors in monocytes from patients with bronchial asthma.
abstract: Adenosine is generated from adenosine triphosphate, which is released by stressed and damaged cells. Adenosine levels are significantly increased in patients with bronchial asthma (BA) and mediate mast cell degranulation and bronchoconstriction. Over the last decade, increasing evidence has shown that adenosine can modulate the innate immune response during monocytes differentiation towards mature myeloid cells. These adenosine-differentiated myeloid cells, characterized by co-expression of monocytes/macrophages and dendritic cell markers such as CD14 and CD209, produce high levels of pro-inflammatory cytokines, thus contributing to the pathogenesis of BA and chronic obstructive pulmonary disease. We found that expression of ADORA2A and ADORA2B are increased in monocytes obtained from patients with BA, and are associated with the generation of CD14(pos)CD209(pos) pro-inflammatory cells. A positive correlation between expression of ADORA2B and IL-6 was identified in human monocytes and may explain the increased expression of IL-6 mRNA in asthmatics. Taken together, our results suggest that monocyte-specific expression of A2 adenosine receptors plays an important role in pro-inflammatory activation of human monocytes, thus contributing to the progression of asthma.
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Ksenia
authors.initials: K
authors.lastname: Yuryeva
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Saltykova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Ogorodova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Kirillova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Kulikov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Korotkaya
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Iakovleva
authors.affiliation: Cardiovascular Division, Department of Medicine, Vanderbilt University, Nashville, TN, USA.
authors.forename: Igor
authors.initials: I
authors.lastname: Feoktistov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Sazonov
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME, USA. Electronic address: sryzhov@mmc.org.
authors.forename: Sergey
authors.initials: S
authors.lastname: Ryzhov
mesh_terms: D000328:Adult; D001249:Asthma / Q000378:metabolism* / Q000473:pathology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology*; D043683:Receptors, Adenosine A2 / Q000378:metabolism*; D018047:Receptors, Purinergic P1 / Q000378:metabolism*
chemical_list: D016207:Cytokines; D043683:Receptors, Adenosine A2; D018047:Receptors, Purinergic P1
keywords: Adenosine receptors; Bronchial asthma; Dendritic cells; Inflammation; Monocytes