title: Anti-adipogenic effect of Artemisia annua in diet-induced-obesity mice model.
abstract: Obesity has increased continuously in western countries during the last several decades and recently become a problem in developing countries. Currently, anti-obesity drugs originating from natural products are being investigated for their potential to overcome adverse effects associated with chemical drugs. Artemisinic acid, which was isolated from the well-known anti-malaria herb Artemisia annua (AA) L., was recently shown to possess anti-adipogenic effects in vitro. However, the anti-adipogenic effects of AA in animal models have not yet been investigated. Therefore, we conducted daily oral administration with AA water extract in a diet-induced obesity animal model and treated 3T3-L1 cells with AA to confirm the anti-adipogenic effects in the related protein expressions. We then evaluated the physiology, adipose tissue histology and mRNA expressions of many related genes. Inhibition of adipogenesis by the AA water extract was observed in vitro. In the animal model, weight gain was significantly lower in the AA treated group, but there were no changes in food intake volume or calories. Reductions in lipid droplet size and mRNA expression associated with adipogenesis were also observed in animal epididymal fat. This study is the first to report that AA has an anti-obese effects in vivo.
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Baek
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Hyeji
authors.initials: H
authors.lastname: Shim
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Hyunmook
authors.initials: H
authors.lastname: Lim
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Minju
authors.initials: M
authors.lastname: Shim
authors.affiliation: Department of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Chul-Kyu
authors.initials: CK
authors.lastname: Kim
authors.affiliation: Department of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Park
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Genomic Informatic Center, Han-kyong National University, Anseong 17579, Korea.
authors.forename: Ki-Duk
authors.initials: KD
authors.lastname: Song
authors.affiliation: Department of Biotechnology, Hoseo University, Asan 31499, Korea.
authors.forename: Sung-Jo
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea.
authors.forename: Sun Shin
authors.initials: SS
authors.lastname: Yi
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D031172:Artemisia annua / Q000737:chemistry*; D001391:Azo Compounds / Q000737:chemistry; D015153:Blotting, Western; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000502:physiology*; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D001391:Azo Compounds; D010936:Plant Extracts; D012333:RNA, Messenger; C011049:oil red O
keywords: Artemisia annua; adipogenesis; animal model; diet induced obesity; obesity