title: Artificial Loading of ASC Specks with Cytosolic Antigens.
abstract: Inflammasome complexes form upon interaction of Nod Like Receptor (NLR) proteins with pathogen associated molecular patterns (PAPMS) inside the cytosol. Stimulation of a subset of inflammasome receptors including NLRP3, NLRC4 and AIM2 triggers formation of the micrometer-sized spherical supramolecular complex called the ASC speck. The ASC speck is thought to be the platform of inflammasome activity, but the reason why a supramolecular complex is preferred against oligomeric platforms remains elusive. We observed that a set of cytosolic proteins, including the model antigen ovalbumin, tend to co-aggregate on the ASC speck. We suggest that co-aggregation of antigenic proteins on the ASC speck during intracellular infection might be instrumental in antigen presentation.
authors.affiliation: Apoptosis and Cancer Immunology Laboratory (AKiL), Department of Molecular Biology and Genetics, Bogazici University, Istanbul, Turkey.
authors.forename: Ali Can
authors.initials: AC
authors.lastname: Sahillioğlu
authors.affiliation: Apoptosis and Cancer Immunology Laboratory (AKiL), Department of Molecular Biology and Genetics, Bogazici University, Istanbul, Turkey; Life Sciences and Technologies Research Center, Bogazici University, Istanbul, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Özören
mesh_terms: D017951:Antigen Presentation; D000941:Antigens / Q000737:chemistry; D053468:CARD Signaling Adaptor Proteins / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D020170:Caspase 1 / Q000378:metabolism; D002454:Cell Differentiation; D003599:Cytoskeleton / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D007249:Inflammation; D008264:Macrophages / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D010047:Ovalbumin / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000737:chemistry; D025801:Ubiquitin / Q000378:metabolism
chemical_list: C410167:AIM2 protein, human; D000941:Antigens; D053468:CARD Signaling Adaptor Proteins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C435692:NLRC4 protein, human; C453881:NLRP3 protein, human; D025801:Ubiquitin; D049452:Green Fluorescent Proteins; D010047:Ovalbumin; D020170:Caspase 1; D046988:Proteasome Endopeptidase Complex; D013755:Tetradecanoylphorbol Acetate