title: Molecular basis of resistance to macrolides, lincosamides and streptogramins in Staphylococcus hominis strains isolated from clinical specimens.
abstract: Coagulase-negative staphylococci (CoNS) are the most frequently isolated bacteria from the blood and the predominant cause of nosocomial infections. Macrolides, lincosamides and streptogramin B (MLSB) antibiotics, especially erythromycin and clindamycin, are important therapeutic agents in the treatment of methicillin-resistant staphylococci infections. Among CoNS, Staphylococcus hominis represents the third most common organism. In spite of its clinical significance, very little is known about its mechanisms of resistance to antibiotics, especially MLSB. Fifty-five S. hominis isolates from the blood and the surgical wounds of hospitalized patients were studied. The erm(C) gene was predominant in erythromycin-resistant S. hominis isolates. The methylase genes, erm(A) and erm(B), were present in 15 and 25% of clinical isolates, respectively. A combination of various erythromycin resistance methylase (erm) genes was detected in 15% S. hominis isolates. The efflux gene msr(A) was detected in 18% of isolates, alone in four isolates, and in different combinations in a further six. The lnu(A) gene, responsible for enzymatic inactivation of lincosamides was carried by 31% of the isolates. No erythromycin resistance that could not be attributed to the genes erm(A), erm(B), erm(C) and msr(A) was detected. In S. hominis, 75 and 84%, respectively, were erythromycin resistant and clindamycin susceptible. Among erythromycin-resistant S. hominis isolates, 68% of these strains showed the inducible MLSB phenotype. Four isolates harbouring the msr(A) genes alone displayed the MSB phenotype. These studies indicated that resistance to MLSB in S. hominis is mostly based on the ribosomal target modification mechanism mediated by erm genes, mainly the erm(C), and enzymatic drug inactivation mediated by lnu(A).
authors.affiliation: Department of Microbiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614, Poznań, Poland. ewasz@amu.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Szczuka
authors.affiliation: Department of Microbiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614, Poznań, Poland.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Makowska
authors.affiliation: Department of Microbiological and Laboratory Diagnostics, Bacteriological Laboratory, Regional Hospital in Poznań, Juraszów 7/19, 60-479, Poznań, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Bosacka
authors.affiliation: Bacteriological Laboratory, Regional Hospital in Kołobrzeg, E. Łopuskiego 33, 78-100, Kołobrzeg, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Słotwińska
authors.affiliation: Department of Microbiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614, Poznań, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kaznowski
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D055231:Lincosamides / Q000494:pharmacology*; D018942:Macrolides / Q000494:pharmacology*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000382:microbiology*; D041181:Staphylococcus hominis / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D025361:Streptogramins / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D055231:Lincosamides; D018942:Macrolides; D025361:Streptogramins; D008780:Methyltransferases; C431709:ErmA protein, Bacteria