title: Development of a test strip for rapid detection of lactoperoxidase in raw milk.
abstract: Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3',5,5'-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1-2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Che
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tian
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Bai
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xiao-na
authors.initials: XN
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China; zhanmeijiang@neau.edu.cn.
authors.forename: Zhan-mei
authors.initials: ZM
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China; xiaoxi_xu01@163.com.
authors.forename: Xiao-xi
authors.initials: XX
authors.lastname: Xu
mesh_terms: D000818:Animals; D002854:Chromatography, Paper / Q000295:instrumentation; D002863:Chromogenic Compounds / Q000737:chemistry; D004209:Disposable Equipment; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D007784:Lactoperoxidase / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D061352:Pasteurization; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002863:Chromogenic Compounds; D011934:Reagent Strips; D007784:Lactoperoxidase
keywords: 3,3',5,5'-Tetramethylbenzidine (TMB); Lactoperoxidase; Rapid detection; Raw milk; Test strip