title: Establishment of a Zebrafish Infection Model for the Study of Wild-Type and Recombinant European Sheatfish Virus.
abstract: Amphibian-like ranaviruses include pathogens of fish, amphibians, and reptiles that have recently evolved from a fish-infecting ancestor. The molecular determinants of host range and virulence in this group are largely unknown, and currently fish infection models are lacking. We show that European sheatfish virus (ESV) can productively infect zebrafish, causing a lethal pathology, and describe a method for the generation of recombinant ESV, establishing a useful model for the study of fish ranavirus infections.
authors.affiliation: Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Martín
authors.affiliation: Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.
authors.forename: Carla
authors.initials: C
authors.lastname: Mavian
authors.affiliation: Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: López Bueno
authors.affiliation: Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: de Molina
authors.affiliation: Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Díaz
authors.affiliation: Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.
authors.forename: Germán
authors.initials: G
authors.lastname: Andrés
authors.affiliation: Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Alcami
authors.affiliation: Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Spain alejo@inia.es.
authors.forename: Alí
authors.initials: A
authors.lastname: Alejo
mesh_terms: D000818:Animals; D001483:Base Sequence; D004266:DNA Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004195:Disease Models, Animal; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D005818:Genetic Engineering; D005838:Genotype; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017995:Ranavirus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence; D015027:Zebrafish / Q000821:virology*