title: Recent advances in genome editing and creation of genetically modified pigs.
abstract: The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Butler
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Ladowski
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Martens
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Tector
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. Electronic address: jtector@iupui.edu.
authors.forename: A Joseph
authors.initials: AJ
authors.lastname: Tector
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D013552:Swine / Q000235:genetics*; D014183:Transplantation, Heterologous
keywords: Endonuclease; Homologous recombination; Off-target; TALEN CRISPR/Cas9; Zinc finger nuclease