title: Functionalized silica nanoparticles as a carrier for Betamethasone Sodium Phosphate: Drug release study and statistical optimization of drug loading by response surface method.
abstract: Mesoporous silica nanoparticles with a hexagonal structure (SBA-15) were synthesized and modified with (3-aminopropyl) triethoxysilane (APTES), and their performance as a carrier for drug delivery system was studied. Chemical structure and morphology of the synthesized and modified SBA-15 were characterized by SEM, BET, TEM, FT-IR and CHN technique. Betamethasone Sodium Phosphate (BSP) as a water soluble drug was loaded on the mesoporous silica particle for the first time. The response surface method was employed to obtain the optimum conditions for the drug/silica nanoparticle preparation, by using Design-Expert software. The effect of time, pH of preparative media, and drug/silica ratio on the drug loading efficiency was investigated by the software. The maximum loading (33.69%) was achieved under optimized condition (pH: 1.8, time: 3.54 (h) and drug/silica ratio: 1.7). The in vitro release behavior of drug loaded particles under various pH values was evaluated. Finally, the release kinetic of the drug was investigated using the Higuchi and Korsmeyer-Peppas models. Cell culture and cytotoxicity assays revealed the synthesized product doesn't have any cytotoxicity against human bladder cell line 5637. Accordingly, the produced drug-loaded nanostructures can be applied via different routes, such as implantation and topical or oral administration.
authors.affiliation: Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ghasemnejad
authors.affiliation: Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran. Electronic address: ahmadi@znu.ac.ir.
authors.forename: E
authors.initials: E
authors.lastname: Ahmadi
authors.affiliation: Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), P.O. Box 45195-159, Gava Zang, Zanjan, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Mohamadnia
authors.affiliation: Department of Chemical Engineering, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Doustgani
authors.affiliation: Department of Textile Engineering, Functional Fibrous Structures & Environmental Enhancement (FFSEE), Amirkabir University of Technology (Tehran Polytechnic), P.O. Box 15875-4413, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Hashemikia
mesh_terms: D001623:Betamethasone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D012822:Silicon Dioxide; C028994:betamethasone sodium phosphate; D001623:Betamethasone
keywords: Betamethasone Sodium Phosphate (BSP); Drug delivery; Mesoporous silica; Modification; Response surface method