title: Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization.
abstract: A facile method to prepare a biocompatible graphene oxide (GO)-based substrate for protein immobilization was developed to overcome the drawbacks of GO, such as the strong electrostatic and hydrophobic interactions which could potentially alter the conformation and biological activity of proteins. The GO was coated with hydrophilic branched polyethyleneimine (BPEI), while Concanavalin A (Con A) as a model lectin protein was employed to fabricate the functionalized composites to evaluate the feasibility of this strategy. The composites exhibit an extremely high binding capacity for glycoproteins (i.e. IgG 538.3 mg g(-1)), which are superior to other immobilized materials. Moreover, they can work well in 500-fold non-glycoprotein interference and even in complex biological samples. All these data suggest that the GO@BPEI composites will have great potential as scaffolds for proteins fully exerting their biofunctions.
authors.affiliation: National Chromatographic R & A Center, Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yejing
authors.initials: Y
authors.lastname: Weng
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Kaiguang
authors.initials: K
authors.lastname: Yang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Sui
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity; D010087:Oxides / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010087:Oxides; D003208:Concanavalin A; D006108:Graphite; D011094:Polyethyleneimine