title: Strain-level visualized analysis of cold-stressed Vibrio parahaemolyticus based on MALDI-TOF mass fingerprinting.
abstract: In this study, strain-level visualized analysis of cold-stressed Vibrio parahaemolyticus based on matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass fingerprinting was investigated. All the peptide mass fingerprinting profiles obtained were analyzed by self-organized map (SOM) and cluster analysis. Our results showed that the peptide mass fingerprinting profiles of V. parahaemolyticus substantially changed under cold stress at strain level. The cold-stressed V. parahaemolyticus strains were distributed to 14 neurons by SOM classification, almost totally different from the controls. This is the first time that so many strains had been chosen to study bacterial cold stress responses, which can help promote an overall understanding to stress responses of cold-stressed strains.
authors.affiliation: Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Xuan
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China. Electronic address: jiajuntao@hotmail.com.
authors.forename: Juntao
authors.initials: J
authors.lastname: Jia
authors.affiliation: Research Institute for Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Xu
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Liang
authors.affiliation: Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Li
mesh_terms: D016000:Cluster Analysis; D003080:Cold Temperature; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000145:classification / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D020543:Proteome
keywords: Cold stress; MALDI-typing; Peptide mass fingerprinting; Phyloproteomic analysis; Self-organized map; Vibrio parahaemolyticus; Visualized analysis