title: Design and Facile Synthesis of New Dinucleotide Cap Analog Containing Both 2' and 3'-OH Modification on M⁷Guanosine Moiety.
abstract: The first example of the synthesis of new dinucleotide cap analog containing 2('),3(')-diacetyl group on m(7)guanosine moiety is described. The desired modified cap analog, m(7,2)(')(,3)(')(-diacetyl)G[5(')]ppp[5(')]G has been obtained by the coupling reaction of triethylamine salt of m(7,2)(')(,3)(')(-diacetyl)GDP with ImGMP in presence of ZnCl2 as a catalyst in 62% yield with high purity. The structure of new cap analog has been confirmed by (1)H and (31)P NMR and mass data.
authors.affiliation: a Life Sciences Solutions Group, Thermo Fisher Scientific , Austin , Texas , USA.
authors.forename: Anilkumar R
authors.initials: AR
authors.lastname: Kore
authors.forename: Alejandro
authors.initials: A
authors.lastname: Bugarin
authors.forename: Muthian
authors.initials: M
authors.lastname: Shanmugasundaram
mesh_terms: D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D012314:RNA Cap Analogs / Q000138:chemical synthesis / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry
chemical_list: D012314:RNA Cap Analogs; D012333:RNA, Messenger; D006151:Guanosine
keywords: 5′-capped mRNA; Cap analog; dendritic cells; diacetyl cap analog