title: [Concentration of pro-inflammatory interleukins in cervical secretions of women with PROM and in the umbilical cord blood of their newborns].
abstract: OBJECTIVE
  The aim of the study was to analyze the levels of pro-inflammatory interleukins in cervical secretions of women with PROM, depending on Ureaplasma spp. infection and the time elapsed since the rupture of the membranes, and to correlate their concentration in cervical secretions and in cord blood of the newborns.
  
  
  METHODS
  The study included 30 women with PROM between 24 and 33+6 weeks of gestation. Cervical swabs from women with confirmed rupture of membranes taken at certain intervals and umbilical cord blood of their newborns constituted the study material. Cervical secretions were evaluated microbiologically and by the PCR method. Concentrations of IL-6, IL-19, IL-10 and TNF-α were analyzed by ELISA.
  
  
  RESULTS
  Ureaplasma spp. were the most frequently isolated microorganisms in cervical secretions of women with PROM. Secretion of interleukins in the cervix was not influenced by time elapsed since the PROM. Comparison of interleukin levels in cord blood of newborns born to mothers with and without Ureaplasma spp infection revealed significantly higher levels of IL-6 in the case of Ureaplasma spp. A positive correlation between IL-6 and TNF-α levels in cervical secretions and in cord blood of mothers with PROM and Ureaplasma spp. was detected.
  
  
  CONCLUSIONS
  1. Cervical culture method appears to be sufficient for detecting Ureaplasma spp. 2. Pro-inflammatory interleukins, especially IL-6, obtained by non-invasive methods can be used to predict fetal inflammatory response.
authors.forename: Jana
authors.initials: J
authors.lastname: Skrzypczak
authors.forename: Przemysław K
authors.initials: PK
authors.lastname: Wirstlein
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wróbel
authors.forename: Mateusz
authors.initials: M
authors.lastname: Mikołajczyk
mesh_terms: D015415:Biomarkers / Q000097:blood; D002582:Cervix Mucus / Q000737:chemistry*; D002584:Cervix Uteri / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000737:chemistry* / Q000378:metabolism; D005322:Premature Rupture of Fetal Membranes / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007378:Interleukins / Q000097:blood*; D011247:Pregnancy; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; C421250:IL19 protein, human; D015850:Interleukin-6; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10