title: The effect of lead on the growth, content of primary metabolites, and antioxidant response of green alga Acutodesmus obliquus (Chlorophyceae).
abstract: Green unicellular alga Acutodesmus obliquus (Turpin) Hegewald et Hanagata (SAG strain no. 276-6) (Chlorophyceae) was used for determination of phytotoxicity of lead (Pb) at the range of concentrations 0.01-500 μM during 7 days of culture. The accumulation of Pb in algal cells was found to be increased in a concentration- and duration-dependent manner. The highest Pb uptake value was obtained in response to 500 μM Pb on the seventh day of cultivation. The decrease in the number and the size of cells and the contents of selected primary metabolites (photosynthetic pigments, monosaccharides, and proteins) in A. obliquus cells were observed under Pb stress. Heavy metal stimulated also formation of reactive oxygen species (hydrogen peroxide) and oxidative damage as evidenced by increased lipid peroxidation. On the other hand, the deleterious effects of Pb resulting from the cellular oxidative state can be alleviated by enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbate, glutathione) antioxidant systems. These results suggest that A. obliquus is a promising bioindicator of heavy metal toxicity in aquatic environment, and it has been identified as good scavenger of Pb from aqueous solution.
authors.affiliation: Department of Plant Biochemistry and Toxicology, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245, Bialystok, Poland. alicjap@uwb.edu.pl.
authors.forename: Alicja
authors.initials: A
authors.lastname: Piotrowska-Niczyporuk
authors.affiliation: Department of Plant Biochemistry and Toxicology, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245, Bialystok, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bajguz
authors.affiliation: Department of Plant Biochemistry and Toxicology, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245, Bialystok, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Talarek
authors.affiliation: Department of Plant Biochemistry and Toxicology, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245, Bialystok, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Bralska
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Zambrzycka
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007854:Lead / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D007854:Lead; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Antioxidants; Growth; Lead; Oxidative stress; Toxicity