title: Assessment of DNA Integrity, Applications for Cancer Research.
abstract: Many methods have been developed for DNA integrity assessment including electrophoresis-based procedures, quantitative PCR, and, more recently, microfluidics-based procedures. DNA integrity evaluation can be employed for characterizing biological samples quality before extensive genomic analysis and also finds applications in reproductive medicine, prenatal diagnostics, or cancer research. In this chapter, we will focus on the assessment of DNA integrity in cancer research. In particular, we will present the application of the determination of DNA integrity for tracking of circulating tumor DNA. Finally, we will conclude by illustrating the potential innovative application of DNA integrity as a biomarker in clinical research, especially for prognostic purposes, patient follow-up, or early diagnosis.
authors.affiliation: Université Paris Sorbonne Cité, INSERM UMR-S1147, Centre Universitaire des Saints-Pères, Paris Cedex 06, France.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Zonta
authors.affiliation: Université Paris Sorbonne Cité, INSERM UMR-S1147, Centre Universitaire des Saints-Pères, Paris Cedex 06, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Nizard
authors.affiliation: Université Paris Sorbonne Cité, INSERM UMR-S1147, Centre Universitaire des Saints-Pères, Paris Cedex 06, France. Electronic address: valerie.taly@parisdescartes.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Taly
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000097:blood* / Q000235:genetics*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm
keywords: ALU elements; Cancer biomarker; Cell-free circulating DNA; Circulating tumor DNA; DNA integrity index; DNA quality; DNA size profiling; dPCR; qPCR