title: A new label-free strategy for a highly efficient chemiluminescence immunoassay.
abstract: A new label-free chemiluminescence (CL) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. The specific immunoreaction on the interface effectively inhibits the enzymatic CL reaction, thus causing a decrease in CL signals.
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, P. R. China. zjyang@yzu.edu.cn.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Juntao
authors.initials: J
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Du
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
authors.forename: Yuehe
authors.initials: Y
authors.lastname: Lin
mesh_terms: D006735:Horseradish Peroxidase / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins; D006735:Horseradish Peroxidase