title: A time-course study of long term over-expression of ARR19 in mice.
abstract: A leucine-rich protein, ARR19 (androgen receptor corepressor-19 kDa), is highly expressed in male reproductive organs and moderately in others. Previously, we have reported that ARR19 is differentially expressed in adult Leydig cells during the testis development and inhibits steroidogenesis by reducing the expression of steroidogenic enzymes. Whereas in prostate, ARR19 represses the transcriptional activity of AR (androgen receptor), it is important for male sexual differentiation and maturation in prostate and epididymis, through the recruitment of HDAC4. In this study we show that long term adenovirus mediated overexpression of ARR19 in mice testis has the potential of inhibiting the differentiation of testicular and prostatic cells by reducing the size of testis and prostate but has no effect on the growth of seminal vesicles. Further, it reduces the level of progesterone and testosterone by reducing the steroidogenic enzymes such as 3HSD, P450c17 and StAR. This is the first study reporting a time-course analysis of the implications of long term overexpression of ARR19 in mice testis and its effect on other organs such as prostate and seminal vesicles. Taken together, these results suggest that ARR19 may play an important role in the differentiation of male reproductive organs such as testis and prostate.
authors.affiliation: School of Biotechnology, Gautam Buddha University, Greater Noida-201308, India.
authors.forename: Imteyaz
authors.initials: I
authors.lastname: Qamar
authors.affiliation: School of Biotechnology, Jawaharlal Nehru University, New Delhi-110067, India.
authors.forename: Mohammad Faiz
authors.initials: MF
authors.lastname: Ahmad
authors.affiliation: Department of Life Science, Research Center for Cell Homeostasis, Ewha Womens University, Seoul 120-750, Republic of Korea.
authors.forename: Arul
authors.initials: A
authors.lastname: Narayanasamy
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D004822:Epididymis / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006655:Histone Deacetylases / Q000235:genetics; D006801:Humans; D062792:MARVEL Domain-Containing Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D011374:Progesterone / Q000378:metabolism; D011467:Prostate / Q000254:growth & development* / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D013737:Testis / Q000254:growth & development* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
chemical_list: C489881:Cmtm2a protein, mouse; D062792:MARVEL Domain-Containing Proteins; D011944:Receptors, Androgen; D012097:Repressor Proteins; D013739:Testosterone; D011374:Progesterone; C498618:Hdac5 protein, mouse; D006655:Histone Deacetylases