title: Morphological characterization of Class III phosphoinositide 3-kinase during mouse brain development.
abstract: The mammalian Class III phosphoinositide 3-kinase (PIK3C3, also known as mammalian vacuolar protein sorting 34 homologue, Vps34) is a regulator of vesicular trafficking, autophagy, and nutrient sensing. In this study, we generated a specific antibody against PIK3C3, and carried out expression and morphological analyses of PIK3C3 during mouse brain development. In Western blotting, PIK3C3 was detected throughout the developmental process with higher expression in the early embryonic stage. In immunohistochemical analyses with embryonic day 16 mouse brain, PIK3C3 was detected strongly in the axon of cortical neurons. While PIK3C3 was distributed at the soma, nucleus, axon, and dendrites in primary cultured mouse hippocampal neurons at 3 days in vitro (div), it was also found in a punctate distribution with partial colocalization with synaptic marker, synaptophysin, at 21 div. The obtained results indicate that PIK3C3 is expressed and may have a physiological role in central nervous system during corticogenesis.
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Inaguma
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ito
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Iwamoto
authors.affiliation: Department of Pediatrics, Jichi Medical University, Tochigi, 329-0498, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Department of Pediatrics, Jichi Medical University, Tochigi, 329-0498, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Yamagata
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tabata
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan. knagata@inst-hsc.jp.
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000818:Animals; D001369:Axons / Q000201:enzymology; D001921:Brain / Q000196:embryology / Q000201:enzymology*; D019556:COS Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D058546:Class III Phosphatidylinositol 3-Kinases; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology; D008813:Mice, Inbred ICR; D009474:Neurons / Q000201:enzymology*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D016708:Synaptophysin / Q000378:metabolism
chemical_list: D058546:Class III Phosphatidylinositol 3-Kinases; D019869:Phosphatidylinositol 3-Kinases; D016708:Synaptophysin; C569015:Syp protein, mouse; C000611846:PIK3C3 protein, mouse
keywords: Antibody; Cerebral cortex; Class III phosphoinositide 3-kinase; Development