title: Association of FcγRIIa Polymorphism with Clinical Outcome of Dengue Infection: First Insight from Pakistan.
abstract: Dengue illness has been a major health concern in Pakistan during the last decade. Dengue infection can result in a spectrum of clinically distinct outcomes, ranging from asymptomatic infection to potentially life-threatening forms of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). A single-nucleotide polymorphism in FcγRIIa (rs1801274) results in altered affinity of the receptor for different subclasses of immunoglobulin G, and is a key player in determining the susceptibility to or protection from severe clinical infection of dengue. In this study, we analyzed the allelic and genotypic distribution of rs1801274 in subjects of Pakistani origin with subclinical dengue infection (n = 40), dengue fever (DF) (n = 40), and DHF/DSS (n = 30). We found that HH homozygotes and heterozygotes were significantly more likely to develop clinical dengue (odds ratio [OR] = 3.21, 95% confidence interval [CI] = 1.29-7.97, P = 0.009), either DF (OR = 2.82, 95% CI = 1.00-7.97, P = 0.045) or DHF/DSS (OR = 3.90, 95% CI = 1.13-13.07, P = 0.024) than the asymptomatic dengue infection. Results of allelic distribution comparisons and logistic regression analysis also supported the same relationship. The results suggest complex nature of interacting factors in determining the course for severe dengue illness.
authors.affiliation: Pakistan Medical Research Council Research Centre, National Health Research Complex, Shaikh Zayed Medical Complex, Lahore, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan; Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Saima Naz
authors.initials: SN
authors.lastname: Mohsin
authors.affiliation: Pakistan Medical Research Council Research Centre, National Health Research Complex, Shaikh Zayed Medical Complex, Lahore, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan; Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Saqib
authors.initials: S
authors.lastname: Mahmood
authors.affiliation: Pakistan Medical Research Council Research Centre, National Health Research Complex, Shaikh Zayed Medical Complex, Lahore, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan; Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan ali.amar@uhs.edu.pk.
authors.forename: Ali
authors.initials: A
authors.lastname: Amar
authors.affiliation: Pakistan Medical Research Council Research Centre, National Health Research Complex, Shaikh Zayed Medical Complex, Lahore, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan; Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Farkhanda
authors.initials: F
authors.lastname: Ghafoor
authors.affiliation: Pakistan Medical Research Council Research Centre, National Health Research Complex, Shaikh Zayed Medical Complex, Lahore, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan; Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Syed Mohsin
authors.initials: SM
authors.lastname: Raza
authors.affiliation: Pakistan Medical Research Council Research Centre, National Health Research Complex, Shaikh Zayed Medical Complex, Lahore, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan; Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Mahjabeen
authors.initials: M
authors.lastname: Saleem
mesh_terms: D000293:Adolescent; D058345:Asymptomatic Infections; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D010154:Pakistan / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017452:Receptors, IgG / Q000235:genetics*; D019595:Severe Dengue / Q000235:genetics; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: C080568:Fc gamma receptor IIA; D017452:Receptors, IgG