title: Raman micro-spectroscopy study of living SH-SY5Y cells adhering on different substrates.
abstract: In this paper we test the ability of Raman micro-spectroscopy and Raman mapping to investigate the status of cells grown in adhesion on different substrates. The spectra of immortalized SH-SY5Y cells, grown on silicon and on metallic substrates are compared with those obtained for the same type of cells adhering on organic polyaniline (PANI), a memristive substrate chosen to achieve a living bio-hybrid system. Raman spectra give information on the status of the single cell, its local biochemical composition, and on the modifications induced by the substrate interaction. The good agreement between Raman spectra collected from cells adhering on different substrates confirms that the PANI, besides allowing the cell growth, doesn't strongly affect the general biochemical properties of the cell. The investigation of the cellular state in a label free condition is challenging and the obtained results confirm the Raman ability to achieve this information.
authors.affiliation: Istituto Officina dei Materiali del CNR (CNR-IOM) - Unità di Perugia, c/o Dipartimento di Fisica e Geologia, Via Pascoli, Perugia, Italy. Electronic address: silvia.caponi@cnr.it.
authors.forename: S
authors.initials: S
authors.lastname: Caponi
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Mattana
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy; Istituto di Biofisica CNR (IBF-CNR), Unità di Trento, & FBK, Via Sommarive 18, 38123 Trento, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ricci
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy.
authors.forename: K
authors.initials: K
authors.lastname: Sagini
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Urbanelli
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Sassi
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Morresi
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di sotto 8, 06123 Perugia, Italy; Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), Università di Perugia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Emiliani
authors.affiliation: Istituto di Biofisica CNR (IBF-CNR), Unità di Trento, & FBK, Via Sommarive 18, 38123 Trento, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Dalla Serra
authors.affiliation: CNR-IMEM (National Research Council, Institute of Materials for Electronics and Magnetism), University of Parma, Viale Usberti 7A, 42124 Parma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Iannotta
authors.affiliation: Istituto di Biofisica CNR (IBF-CNR), Unità di Trento, & FBK, Via Sommarive 18, 38123 Trento, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Musio
authors.affiliation: Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), Università di Perugia, Italy; Dipartimento di Fisica e Geologia, Via Pascoli, Università di Perugia, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Fioretto
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D012825:Silicon / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D014407:Tumor Cells, Cultured
chemical_list: D000814:Aniline Compounds; C416807:polyaniline; D012825:Silicon
keywords: Cells adhering on different substrates; Cellular mapping; Cellular stress; Neuron-like cells; Raman micro-spectroscopy