title: Effect of argan oil on liquid storage of ram semen in Tris or skim milk based extenders.
abstract: Due to its high antioxidant content, the argan oil could play a beneficial role in liquid storage of ram semen. The aim of this study was to investigate effects of different concentration of argan oil (ARO) on spermatologic parameters, lipid peroxidation and DNA fragmentation during liquid storage of ram semen until 48 h. Also effects of extenders and temperature on same parameters were assessed. For these aims, semen samples were collected from Boujaâd rams, extended with Tris egg yolk or skim milk extenders without (control) or supplemented with different concentrations of ARO (1%, 2%, 5% and 10% v/v) at a final concentration of 0.8 × 10(9) sperm/mL and stored until 48 h at 5 °C or 15 °C. The sperm quality assessments were performed at different intervals during storage (0, 8, 24 and 48 h). Sperm progressive motility started to decrease after 8h of storage in all temperatures--extenders combinations and dropped steadily during the 8-48 h interval. However, sperm viability, progressive motility and membrane integrity were markedly higher in ARO groups (especially in 1% in Tris and 5% in skim milk) until 24h and 48 h storage at both temperatures compared to controls. The argan oil also decreased the level of spontaneous and induced malondialdehyde (MDA) and the sperm DNA fragmentation until 48 h storage. In conclusion, it was determined that addition of argan oil to conventional extenders may improve the quality of ram semen during liquid storage in different temperatures.
authors.affiliation: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589 Settat, Morocco; Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco.
authors.forename: Larbi
authors.initials: L
authors.lastname: Allai
authors.affiliation: INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Druart
authors.affiliation: Recherche/Development Department, PROISER S.L. Catedrático Agustín Escardino, 9, Building 3, 1st Floor, 46980 Paterna, Valencia, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Contell
authors.affiliation: Biologie Médicale et Biologie de la Reproduction, LABOMAC, Casablanca, Maroc, France.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Louanjli
authors.affiliation: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589 Settat, Morocco.
authors.forename: Anass Ben
authors.initials: AB
authors.lastname: Moula
authors.affiliation: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589 Settat, Morocco; Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco.
authors.forename: Abdelmoughit
authors.initials: A
authors.lastname: Badi
authors.affiliation: Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco.
authors.forename: Abdelkhalid
authors.initials: A
authors.lastname: Essamadi
authors.affiliation: Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco.
authors.forename: Boubker
authors.initials: B
authors.lastname: Nasser
authors.affiliation: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589 Settat, Morocco. Electronic address: bouchraelamiri@hotmail.com.
authors.forename: Bouchra
authors.initials: B
authors.lastname: El Amiri
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008892:Milk / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000662:veterinary*; D012756:Sheep; D014325:Tromethamine / Q000494:pharmacology*
chemical_list: D010938:Plant Oils; D014325:Tromethamine; C555574:argan oil
keywords: Argan oil; Boujaâd rams; DNA fragmentation; Liquid storage (chilling); MDA; Sperm parameters