title: Growth Impairment Caused by Raw Linseed Consumption: Can Trypsin Inhibitors Be Harmful for Health?
abstract: Linseed (Linun usitatissimum L.) is an important oilseed whose nutritional value can be impaired due to presence of antinutritional factors and low protein digestibility. Protein fractions from raw linseed meal were extracted, isolated and analyzed in vitro and in vivo. Globulins, the major protein fraction of linseed, showed low in vitro susceptibility to trypsin and chymotrypsin, but its in vivo digestibility was 93.2 %. Albumin fraction had high trypsin inhibition activity (5250 Inhibition Units g(-1)) and presented low molecular mass protein bands, similar to known trypsin inhibitors. Raw linseed consumption caused negative effects on rat growth and reduction of intestinal villi. Results indicate that raw linseed meal must not be used as an exclusive source of protein regardless of the major proteins have high digestibility; digestive enzymes inhibitors in raw linseed probably reduces the protein utilization.
authors.affiliation: Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil, katya_jacinto@yahoo.com.br.
authors.forename: Katya
authors.initials: K
authors.lastname: Anaya
authors.forename: Ana C B
authors.initials: AC
authors.lastname: Cruz
authors.forename: Dayse C S
authors.initials: DC
authors.lastname: Cunha
authors.forename: Sandra M N
authors.initials: SM
authors.lastname: Monteiro
authors.forename: Elizeu A
authors.initials: EA
authors.lastname: Dos Santos
mesh_terms: D000818:Animals; D001682:Biological Availability; D002918:Chymotrypsin / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology*; D004063:Digestion; D019597:Flax / Q000737:chemistry*; D006128:Growth / Q000187:drug effects*; D006801:Humans; D007422:Intestines / Q000187:drug effects; D008297:Male; D008970:Molecular Weight; D009753:Nutritive Value; D010936:Plant Extracts; D010940:Plant Proteins / Q000378:metabolism / Q000494:pharmacology*; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000009:adverse effects*
chemical_list: D004044:Dietary Proteins; D010936:Plant Extracts; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D014357:Trypsin