title: Simple and rapid determination of free fatty acids in brown rice by FTIR spectroscopy in conjunction with a second-derivative treatment.
abstract: A simple and rapid method for the determination of free fatty acid (FFA) content in brown rice using Fourier transform infrared spectroscopy (FTIR) in conjunction with second-derivative treatment was proposed. Ground brown rice (10g) was soaked in toluene (20mL) for 30min, and the filtrate of the extract was placed in a 1mm CaF2 liquid cell. The transmittance spectrum of the filtrate was recorded using toluene for the background spectrum. The absorption band due to the CO stretching mode of FFAs was detected at 1710cm(-1), and the Savitzky-Golay second-derivative treatment was performed for band separation. A single linear regression model for FFA was developed using the 1710cm(-1) band in the second-derivative spectra of oleic acid in toluene (0.25-2.50gL(-1)), and the model displayed high prediction accuracy with a determination coefficient of 0.998 and a root mean square error of 0.03gL(-1).
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Electronic address: genkawa.takuma.fm@u.tsukuba.ac.jp.
authors.forename: Takuma
authors.initials: T
authors.lastname: Genkawa
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Tofael
authors.initials: T
authors.lastname: Ahamed
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Ryozo
authors.initials: R
authors.lastname: Noguchi
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Takigawa
authors.affiliation: School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Ozaki
mesh_terms: D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005230:Fatty Acids, Nonesterified
keywords: Brown rice; FTIR spectroscopy; Fat acidity; Free fatty acid; Second-derivative treatment