title: Branched zinc oxide nanorods arrays modified paper electrode for electrochemical immunosensing by combining biocatalytic precipitation reaction and competitive immunoassay mode.
abstract: Branched zinc oxide nanorods (BZR) arrays, an array with high charge carries collection efficiency and specific surface area, are grown on the reduced graphene oxide-paper working electrode for the first time to construct a paper-based electrochemical (EC) immunosensor. Typically, the BZR are fabricated via a simple hydrothermal process, which can provide abundant sites for antibodies loading. By combining the large surface area of porous zinc oxide (PZS) and good biocompatibility of gold nanoparticles (AuNPs), PZS-AuNPs (PZS@Au) nanocomposites are designed to label horseradish peroxide (HRP) and antigens. After a competitive reaction between antigens and PZS@Au nanocomposites labeled antigens, the signal labels are introduced into the immunosensor, in which, HRP participate in biocatalytic precipitation process. The produced precipitate reduces the electrode surface area and hinders the electron transfer. With the increase of concentration of antigens, the signal labels introduced into the sensor decrease, thus, a signal-on immunoassay for α-fetoprotein detection is constructed. The proposed paper-based EC immunosensor combines enzymatic biocatalytic precipitation reaction and competitive immunoassay mode for the first time, and possesses a wide linear range from 0.2 pg mL(-1) to 500 ng mL(-1) with a detection limit of 0.08 pg mL(-1). In addition, the proposed method is simple, sensitive and specific and can be a promising platform for other protein detection.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Sun
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China; Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China. Electronic address: chm_gesg@163.com.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.affiliation: Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, Jinan 250117, China.
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
mesh_terms: D000941:Antigens / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000941:Antigens; D000509:alpha-Fetoproteins; D006046:Gold; D006108:Graphite; D015034:Zinc Oxide
keywords: Biocatalytic precipitation; Branched zinc oxide nanorods; Competitive immunoassay mode; Electrochemical