title: A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization.
abstract: An electrochemical biosensor was developed for Hg(2+) determination based on DNA hybridization. In the presence of Hg(2+), the target and probe DNAs with thymine-thymine (T-T) mismatches could hybridize by forming T-Hg(2+)-T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg(2+). The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg(2+) in the range of 9.0 × 10(-11)-1.0 × 10(-9) M. The detection limit was 3.08 × 10(-11) M.
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ebrahimi
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Electronic address: j.raoof@umz.ac.ir.
authors.forename: Jahan Bakhsh
authors.initials: JB
authors.lastname: Raoof
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ojani
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bagheryan
mesh_terms: D020137:Base Pair Mismatch / Q000187:drug effects; D015374:Biosensing Techniques; D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques; D007492:Iran; D008628:Mercury / Q000032:analysis* / Q000494:pharmacology; D009693:Nucleic Acid Hybridization / Q000187:drug effects*; D009841:Oligonucleotides / Q000737:chemistry; D009994:Osmolar Concentration; D011071:Poly T / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D055672:Static Electricity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000494:pharmacology
chemical_list: D004396:Coloring Agents; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D000644:Quaternary Ammonium Compounds; D014874:Water Pollutants, Chemical; D011071:Poly T; D004247:DNA; D008628:Mercury; C006798:brilliant green
keywords: DNA hybridization; Ethyl green; Mercury ions biosensor; Silver nanoparticles; T–Hg(2)(+)–T complex