title: Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system.
abstract: We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Seung-Soo
authors.initials: SS
authors.lastname: Han
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Jinjoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Yideul
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Myeong-Ho
authors.initials: MH
authors.lastname: Kang
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Jung-Hyub
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Min-Suk
authors.initials: MS
authors.lastname: Cha
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Ezra
authors.initials: E
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Yoon
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea. Electronic address: ysbae04@skku.edu.
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Bae
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D005822:Genetic Vectors; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D017955:Poliovirus / Q000235:genetics*; D016019:Survival Analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014615:Vaccinia / Q000517:prevention & control
chemical_list: D016915:AIDS Vaccines; D016655:HIV Core Protein p24; D014614:Vaccines, Synthetic
keywords: HIV-1 p24; Oral CTL vaccine; Poliovirus Sabin 1 vector; RPS-CTP CTL vector; Recombinant poliovirus; p24-specific CTL