title: Comparison of bacterial diversity in full scale anammox bioreactors operated under different conditions.
abstract: Bacterial community structure of full-scale anammox bioreactor is still mainly unknown. It has never been analyzed whether different anammox bioreactor configurations might result in the development of different bacterial community structures among these systems. In this work, the bacterial community structure of six full-scale autotrophic nitrogen removal bioreactors located in The Netherlands and China operating under three different technologies and with different influent wastewater characteristics was studied by the means of pyrotag sequencing evaluation of the bacterial assemblage yielded a great diversity in all systems. The most represented phyla were the Bacteroidetes and the Proteobacteria, followed by the Planctomycetes. 14 OTUs were shared by all bioreactors, but none of them belonged to the Brocadiales order. Statistical analysis at OTU level showed that differences in the microbial communities were high, and that the main driver of the bacterial assemblage composition was different for the distinct phyla identified in the six bioreactors, depending on bioreactor technology or influent wastewater characteristics.
authors.affiliation: Dept. of Civil Engineering, University of Granada, Campus De Fuentenueva, S/N, Granada, 18701, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Gonzalez-Martinez
authors.affiliation: Dept. of Civil Engineering, University of Granada, Campus De Fuentenueva, S/N, Granada, 18701, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Osorio
authors.affiliation: Inst. of Water Research, University of Granada, C/Ramón y Cajal, 4, Granada, 18701, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Morillo
authors.affiliation: Inst. of Water Research, University of Granada, C/Ramón y Cajal, 4, Granada, 18701, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rodriguez-Sanchez
authors.affiliation: Inst. of Water Research, University of Granada, C/Ramón y Cajal, 4, Granada, 18701, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Gonzalez-Lopez
authors.affiliation: Dept. of Biotechnology, Technical University of Delft, Julianalaan 67, Delft, 2628 BC, The Netherlands.
authors.forename: Ben A
authors.initials: BA
authors.lastname: Abbas
authors.affiliation: Dept. of Biotechnology, Technical University of Delft, Julianalaan 67, Delft, 2628 BC, The Netherlands.
authors.forename: Mark C M
authors.initials: MC
authors.lastname: van Loosdrecht
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D016000:Cluster Analysis; D059013:Microbial Consortia; D009584:Nitrogen / Q000302:isolation & purification* / Q000378:metabolism; D058458:Nitrogen Cycle; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D009584:Nitrogen; D062065:Wastewater
keywords: anammox; bioprocess technology; microbial ecology; nitrogen removal; pyrosequencing; wastewater treatment