title: [PROTEOMIC MASS-SPECTROMETRIC ANALYSIS OF FRESHLY ISOLATED ESCHERICHIA COLI STRAINS].
abstract: OBJECTIVE
  Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli--associants of opportunistic bacteria.
  
  
  METHODS
  20 strains of monopopulation of hemolytic E.coli; 100 strains of monopopulation of non-hemolytic E. coli; 15 non-hemolytic strain, isolated from association with opportunistic bacteria. Protein profiling on MALDI-TOF MS Autoflex "Bruker Daltonik" mass-spectrometer.
  
  
  RESULTS
  Value within species for mass-charge peak with 100% intensity of 9000 Da could be a differential sign for isolation of hemolytic E.coli during mass-spectrometric biotyping.
  
  
  CONCLUSIONS
  Proteomic characteristics of strains from various populations within E.coli are given. Taxon-specific markers for proteomic differentiation of E.coli populations are established.
authors.forename: M N
authors.initials: MN
authors.lastname: Gapon
authors.forename: N R
authors.initials: NR
authors.lastname: Telesmanich
authors.forename: L N
authors.initials: LN
authors.lastname: Ternovskaya
authors.forename: S O
authors.initials: SO
authors.lastname: Chaika
authors.forename: I A
authors.initials: IA
authors.lastname: Chaika
authors.forename: Z I
authors.initials: ZI
authors.lastname: Mikashinovich
authors.forename: T B
authors.initials: TB
authors.lastname: Chernukhina
authors.forename: T I
authors.initials: TI
authors.lastname: Tverdokhlebova
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D013058:Mass Spectrometry; D040901:Proteomics
chemical_list: D029968:Escherichia coli Proteins