title: Efficient anaerobic transformation of raw wheat straw by a robust cow rumen-derived microbial consortium.
abstract: A rumen-derived microbial consortium was enriched on raw wheat straw as sole carbon source in a sequential batch-reactor (SBR) process under strict mesophilic anaerobic conditions. After five cycles of enrichment the procedure enabled to select a stable and efficient lignocellulolytic microbial consortium, mainly constituted by members of Firmicutes and Bacteroidetes phyla. The enriched community, designed rumen-wheat straw-derived consortium (RWS) efficiently hydrolyzed lignocellulosic biomass, degrading 55.5% w/w of raw wheat straw over 15days at 35°C and accumulating carboxylates as main products. Cellulolytic and hemicellulolytic activities, mainly detected on the cell bound fraction, were produced in the earlier steps of degradation, their production being correlated with the maximal lignocellulose degradation rates. Overall, these results demonstrate the potential of RWS to convert unpretreated lignocellulosic substrates into useful chemicals.
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Adèle
authors.initials: A
authors.lastname: Lazuka
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Lucas
authors.initials: L
authors.lastname: Auer
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Bozonnet
authors.affiliation: INRA, UR1213 Herbivores, Centre de Theix, F-63122 St-Genès-Champanelle, France.
authors.forename: Diego P
authors.initials: DP
authors.lastname: Morgavi
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Michael
authors.initials: M
authors.lastname: O'Donohue
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France. Electronic address: hernandg@insa-toulouse.fr.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Hernandez-Raquet
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002417:Cattle; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005260:Female; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D059013:Microbial Consortia; D012417:Rumen / Q000382:microbiology*; D014908:Triticum / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; C036909:lignocellulose; D008031:Lignin
keywords: Anaerobic consortium; Carboxylate production; Cellulase; Lignocellulose bioconversion; Xylanase