title: Experimental investigation and oral bioavailability enhancement of nano-sized curcumin by using supercritical anti-solvent process.
abstract: The biomedical applications of curcumin (CUR) are limited due to its poor oral bioavailability. In this work, CUR nanoparticles were successfully prepared by combining the supercritical anti-solvent (SAS) process with Tween 80 as a solubilizing agent and permeation enhancer. Different processing parameters that can govern the mean particle size and size distribution of nanoparticles were well investigated by manipulating the types of solvents, mixing vessel pressure, mixing vessel temperature, CO2 flow rate, solution flow rate and solution concentration. Solid state characterization was done by Fourier Transform infrared spectroscopy, differential scanning calorimetry, dynamic light scattering, scanning electron microscopy, and powder X-ray diffraction study. Solubility and dissolution profile of SAS-processed CUR were found to be significantly increased in comparison with native CUR. Further, a validated ultra-performance liquid chromatographic method with quadrupole-time of flight-mass spectrometry was developed to investigate the pharmacokinetic parameters after a single oral dose (100mg/kg) administration of CUR (before/after SAS-processed) in male Wistar rats. From the plasma concentration vs. time profile graph, oral bioavailability of SAS-processed CUR was found to be increased approximately 11.6-fold (p<0.001) as compared to native CUR.
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India. Electronic address: md.anwar2008@gmail.com.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Anwar
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India.
authors.forename: Musarrat H
authors.initials: MH
authors.lastname: Warsi
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Mohapatra
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India.
authors.forename: Niyaz
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Now at: LE STUDIUM(®) Fellow at Centre de Biophysique Moléculaire (CBM)-CNRS/University of Orléans, UPR4301, rue Charles Sadron, 45071 Orléans, France.
authors.forename: Sohail
authors.initials: S
authors.lastname: Akhter
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India.
authors.forename: Asgar
authors.initials: A
authors.lastname: Ali
authors.affiliation: Nanoformulation Research Laboratory, Faculty of Pharmacy, Hamdard University, New Delhi, India. Electronic address: farhanja_2000@yahoo.com.
authors.forename: Farhan J
authors.initials: FJ
authors.lastname: Ahmad
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D003474:Curcumin / Q000737:chemistry* / Q000493:pharmacokinetics*; D004867:Equipment Design; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017208:Rats, Wistar; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D053719:Tandem Mass Spectrometry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: D012997:Solvents; D003474:Curcumin
keywords: Curcumin nanoparticle; Pharmacokinetics; Q-TOF-MS/MS; SAS process; Solubility; Tween 80; UPLC