title: In vitro anti-tumor activity of the tanshinone IIA against SKOV3 cells.
abstract: The aim of this study was to determine the anti-tumour activity of tanshinone IIA in SKOV3 cells. Results suggested that tanshinone IIA could significantly inhibit (IC50 value  =  19.6 μM) the proliferation and induce apoptosis of SKOV3 cells as demonstrated by flow cytometry analysis. In addition, tanshinone IIA treatment induced G2/M phase cell cycle arrest in SKOV3 cells. The results of Western blotting indicated that tanshinone IIA can suppress the expression of anti-apoptotic protein Bcl-2, increase (0.28 vs. 0.62) the expression of pro-apoptotic protein Bax (0.83 vs. 0.24) in SKOV3 cells. It can be concluded that the tanshinone IIA may be a possible therapeutic candidate having cytotoxic and anti-tumour potential.
authors.affiliation: a Department of Gynaecology , The First Affiliated Hospital of Shantou University Medical College , Shantou , P.R. China.
authors.forename: Ju
authors.initials: J
authors.lastname: Huang
authors.affiliation: b Department of Urology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lin
authors.affiliation: c Department of Urology , The First Affiliated Hospital of Shantou University Medical College , Shantou , P.R. China.
authors.forename: YingKai
authors.initials: Y
authors.lastname: Hong
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C021751:tanshinone
keywords: SKOV3; Tanshinone IIA; apoptosis; cell