title: Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation.
abstract: The objective of this study was to investigate the odontoblastic differentiation of dental pulp stem cells (DPSC) by biodegradable hydrogels incorporating simvastatin micelles, both in vitro and in vivo. Simvastatin (ST) was incorporated into the micelles of gelatin grafted with L-lactic acid oligomers (LAo) to allow water-solubilization. The simvastatin-LAo-grafted gelatin (LAo-g-gelatin) micelles were mixed with gelatin, followed by chemical crosslinking to form gelatin hydrogels (ST Mi/GH). The ST Mi were released from the gelatin hydrogel granules (GH) through enzymatic degradation. The ST Mi enhanced alkaline phosphatase activity, calcium deposition, and bone morphogenic protein-2 secretion of DPSC. When implanted subcutaneously into mice, the ST Mi/GH treated group exhibited increased dentin sialoprotein and calcium deposition, compared with those treated with GH plus free ST. It is possible to achieve odontoblastic differentiation of DPSC through the controlled release of ST from GH.
authors.affiliation: Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Miyazawa
authors.forename: Tomonori
authors.initials: T
authors.lastname: Matsuno
authors.forename: Kazunari
authors.initials: K
authors.lastname: Asano
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Tazuko
authors.initials: T
authors.lastname: Satoh
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000187:drug effects*; D003782:Dental Pulp; D065546:Drug Liberation; D005786:Gene Expression Regulation / Q000187:drug effects; D020100:Hydrogels / Q000737:chemistry*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D051379:Mice; D008823:Micelles; D009804:Odontoblasts / Q000187:drug effects* / Q000502:physiology; D019821:Simvastatin / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D000960:Hypolipidemic Agents; D008823:Micelles; D019821:Simvastatin