title: Tectonic 1 accelerates gastric cancer cell proliferation and cell cycle progression in vitro.
abstract: Hedgehog (Hh) pathway is important in development and cancer. Hh signaling is constitutively active in gastric cancer. Recently, tectonic 1 (TCTN1) was identified as one regulator of the Hh pathway. In the present study, the biological role of TCTN1 was examined in gastric cancer via an RNA interference lentivirus system. The constructed lentivirus efficiently suppressed TCTN1 expression in three gastric cancer cell lines. The proliferation of gastric cancer cells was significantly inhibited in TCTN1 knockdown cells, as determined by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide and colony formation assays. Furthermore, in order to determine the underlying mechanism, the cell cycle progression of MGC80‑3 cells was analyzed by flow cytometry. Knockdown of TCTN1 led to cell cycle arrest at the G2/M phase, which contributed to inhibition of growth. In conclusion, the results demonstrated that TCTN1 was essential in the growth of gastric cancer cells in vitro, suggesting TCTN1 as a potential target candidate for the treatment of gastric cancer.
authors.affiliation: Department of Abdominal Tumor Surgery, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China.
authors.forename: Xinbao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Abdominal Tumor Surgery, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China.
authors.forename: Qiming
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Clinic, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cancer Research Institute, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ling
authors.affiliation: Department of Abdominal Tumor Surgery, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Yu
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; C561486:Tctn1 protein, human