title: The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process.
abstract: Human adipose tissue is a great source of autologous mesenchymal stem cells (hASCs), which are recognized for their vast therapeutic applications. Their ability to self-renew and differentiate into several lineages makes them a promising tool for cell-based therapies in different types of degenerative diseases. Thus it is crucial to evaluate age-related changes in hASCs, as the elderly are a group that will benefit most from their considerable potential. In this study we investigated the effect of donor age on growth kinetics, cellular senescence marker levels, and osteogenic and adipogenic potential of hASCs. It also has been known that, during life, organisms accumulate oxidative damage that negatively affects cell metabolism. Taking this into consideration, we evaluated the levels of nitric oxide, reactive oxygen species, and superoxide dismutase activity. We observed that ROS and NO increase with aging, while SOD activity is significantly reduced. Moreover cells obtained from older patients displayed senescence associated features, for example, β-galactosidase activity, enlarged morphology, and p53 protein upregulation. All of those characteristics seem to contribute to decreased proliferation potential of those cells. Our results suggest that due to aging some cellular modification may be required before applying aged cells efficiently in therapies such as tissue engineering and regenerative medicine.
authors.affiliation: Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kornicka
authors.affiliation: Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland ; Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Department of Anatomy, Jagiellonian University Medical College, 31-034 Krakow, Poland.
authors.forename: Krzysztof Andrzej
authors.initials: KA
authors.lastname: Tomaszewski
authors.affiliation: Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland ; Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland ; Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śmieszek
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000367:Age Factors; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D017382:Reactive Oxygen Species; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D015675:Osteocalcin; D053495:Osteopontin; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D000469:Alkaline Phosphatase