title: Rumen fermentation and acetogen population changes in response to an exogenous acetogen TWA4 strain and Saccharomyces cerevisiae fermentation product.
abstract: The presence of yeast cells could stimulate hydrogen utilization of acetogens and enhance acetogenesis. To understand the roles of acetogens in rumen fermentation, an in vitro rumen fermentation experiment was conducted with addition of acetogen strain (TWA4) and/or Saccharomyces cerevisiae fermentation product (XP). A 2×2 factorial design with two levels of TWA4 (0 or 2×10(7) cells/ml) and XP (0 or 2 g/L) was performed. Volatile fatty acids (VFAs) were increased (P<0.05) in XP and TWA4XP, while methane was increased only in TWA4XP (P<0.05). The increase rate of microorganisms with formyltetrahydrofolate synthetase, especially acetogens, was higher than that of methanogens under all treatments. Lachnospiraceae was predominant in all acetogen communities, but without close acetyl-CoA synthase (ACS) amino acid sequences from cultured isolates. Low-Acetitomaculum ruminis-like ACS was predominant in all acetogen communities, while four unique phylotypes in XP treatment were all amino acid identified low-Eubacterium limosum-like acetogens. It differs to XP treatment that more low-A. ruminis-like and less low-E. limosum-like sequences were identified in TWA4 and TWA4XP treatments. Enhancing acetogenesis by supplementation with an acetogen strain and/or yeast cells may be an approach to mitigate methane, by targeting proper acetogens such as uncultured low-E. limosum-like acetogens.
authors.affiliation: Institute of Dairy Science, MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chun-lei
authors.initials: CL
authors.lastname: Yang
authors.affiliation: Department of Agricultural, Food & Nutritional Science, Faculty of Agricultural, Life & Environmental Sciences, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
authors.forename: Le-luo
authors.initials: LL
authors.lastname: Guan
authors.affiliation: Institute of Dairy Science, MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Liu
authors.affiliation: Institute of Dairy Science, MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; jiakunwang@zju.edu.cn.
authors.forename: Jia-kun
authors.initials: JK
authors.lastname: Wang
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005285:Fermentation / Q000502:physiology*; D059013:Microbial Consortia / Q000502:physiology*; D012417:Rumen / Q000382:microbiology* / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013045:Species Specificity
chemical_list: D000085:Acetates
keywords: Acetogen; Methanogen; Rumen fermentation; Saccharomyces cerevisiae fermentation product