title: Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd(3+)-DOTA.
abstract: Gd(3+) is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd(3+)-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.
authors.affiliation: Department of Chemistry, University of Konstanz, Universitätsstr. 10, Konstanz, Germany. joerg.hartig@uni-konstanz.de malte.drescher@uni-konstanz.de.
authors.forename: Filip
authors.initials: F
authors.lastname: Wojciechowski
authors.forename: Andreas
authors.initials: A
authors.lastname: Groß
authors.forename: Isabelle T
authors.initials: IT
authors.lastname: Holder
authors.forename: Laura
authors.initials: L
authors.lastname: Knörr
authors.forename: Malte
authors.initials: M
authors.lastname: Drescher
authors.forename: Jörg S
authors.initials: JS
authors.lastname: Hartig
mesh_terms: D000818:Animals; D057930:Click Chemistry; D004578:Electron Spin Resonance Spectroscopy; D005682:Gadolinium / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000009:adverse effects / Q000138:chemical synthesis*; D009865:Oocytes / Q000378:metabolism; D009942:Organometallic Compounds / Q000737:chemistry*; D013113:Spin Labels; D014817:Vitelline Membrane / Q000187:drug effects; D014982:Xenopus laevis
chemical_list: D006571:Heterocyclic Compounds; D009838:Oligodeoxyribonucleotides; D009942:Organometallic Compounds; D013113:Spin Labels; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D005682:Gadolinium