title: Physico-chemical characterization and cytotoxicity evaluation of curcumin loaded in chitosan/chondroitin sulfate nanoparticles.
abstract: In this study, chitosan (CTS)/chondroitin sulfate (CS) nanoparticles, both pure and curcumin-loaded, were synthesized by ionic gelation. This method is simple and efficient for obtaining nanoparticles with a low polydispersity index (0.151±0.03 to 0.563±0.07) and hydrodynamic diameter in the range of 175.7±2.5 to 710.2±8.9nm, for this study. Samples have a relatively high zeta potential value, a fact that indicates that the colloidal system has good physical and chemical stabilities. The efficiency of the curcumin encapsulation in nanoparticles, which ranged from 62.4±0.61% to 68.3±0.88%, depends on the pH of the chitosan solution. The release of curcumin from the nanoparticles was enabled by a diffusion mechanism, with fast release in a phosphate buffer solution at pH6.8. The assaying of cell viability by the MTT test showed that the presence of both free curcumin and curcumin in the nanoencapsulated form leads to a statistically significant reduction in the viability of A549 cells, by comparison with the control group. The most significant reductions in cell viability of 41.1% and 60.4% (p<0.0001) were observed after 72h, by using 40μmol∙L(-1) free curcumin and curcumin encapsulated in CTS/CS nanoparticles with the chitosan solution at pH6.0, respectively.
authors.affiliation: Universidade de Brasília (UnB) - Campus Planaltina (FUP) - Área Universitária n°01, Vila N. Sa. De Fátima, CEP: 73345-010 - Planaltina, Brasília, DF, Brazil.
authors.forename: Katiúscia Vieira
authors.initials: KV
authors.lastname: Jardim
authors.affiliation: Universidade de Brasília (UnB) - Campus Ceilândia (FCE) - Centro Metropolitano - Conjunto A - Lote 01, CEP: 72220-900 - Ceilândia, Brasília, DF, Brazil.
authors.forename: Graziella Anselmo
authors.initials: GA
authors.lastname: Joanitti
authors.affiliation: Laboratório de Nanobiotecnologia - Instituto de Ciências Biológicas - Universidade de Brasília - UnB - Campus Universitário Darcy Ribeiro - CEP 70910-900 - Asa Norte, Brasília, DF, Brazil.
authors.forename: Ricardo Bentes
authors.initials: RB
authors.lastname: Azevedo
authors.affiliation: Universidade de Brasília (UnB) - Campus Planaltina (FUP) - Área Universitária n°01, Vila N. Sa. De Fátima, CEP: 73345-010 - Planaltina, Brasília, DF, Brazil; Departamento de Química - Universidade Federal de Santa Catarina - CEP: 88040-900 - Trindade, Florianópolis, SC, Brazil. Electronic address: alexandreluisparize@hotmail.com.
authors.forename: Alexandre Luis
authors.initials: AL
authors.lastname: Parize
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; D004337:Drug Carriers; D002809:Chondroitin Sulfates; D048271:Chitosan; D003474:Curcumin
keywords: Chitosan; Chondroitin sulfate; Curcumin; Ionic gelation; Nanoparticles