title: MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression in the prostatic tissue of two ethanol-preferring rat models.
abstract: We investigated whether chronic ethanol intake is capable of altering the MMP-2 and MMP-9 activities and TIMP-2 and TIMP-1 expression in the dorsal and lateral prostatic lobes of low (UChA) and high (UChB) ethanol-preferring rats. MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression were significantly reduced in the lateral prostatic lobe of the ethanol drinking animals. Dorsal prostatic lobe was less affected showing no significant alterations in these proteins, except for a reduction in the TIMP-1 expression in UChA rats. These important findings demonstrate that chronic ethanol intake impairs the physiological balance of the prostate extracellular matrix turnover, through downregulation of MMPs, which may contribute to the development of prostatic diseases. Furthermore, since these proteins are also components of prostate secretion, the negative impact of chronic ethanol intake on fertility may also involve reduction of MMPs and TIMPs in the seminal fluid.
authors.affiliation: Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil ; Structural and Cell Biology Program, UNICAMP, 13083-862 Campinas, SP, Brazil.
authors.forename: Beatriz Aparecida
authors.initials: BA
authors.lastname: Fioruci-Fontanelli
authors.affiliation: Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Luiz Gustavo A
authors.initials: LG
authors.lastname: Chuffa
authors.affiliation: Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil ; Structural and Cell Biology Program, UNICAMP, 13083-862 Campinas, SP, Brazil.
authors.forename: Leonardo O
authors.initials: LO
authors.lastname: Mendes
authors.affiliation: Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Patricia Fernanda F
authors.initials: PF
authors.lastname: Pinheiro
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Flávia Karina
authors.initials: FK
authors.lastname: Delella
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Cilmery S
authors.initials: CS
authors.lastname: Kurokawa
authors.affiliation: Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Sérgio Luis
authors.initials: SL
authors.lastname: Felisbino
authors.affiliation: Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.
authors.forename: Francisco Eduardo
authors.initials: FE
authors.lastname: Martinez
mesh_terms: D000818:Animals; D000431:Ethanol / Q000009:adverse effects*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D023421:Models, Animal; D011467:Prostate / Q000201:enzymology*; D051381:Rats; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D000431:Ethanol; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9