title: Poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate) as an immobilization matrix for microbial glycerol biosensing based on Gluconobacter oxydans.
abstract: A commercial strain of Gluconobacter oxydans together with a new co-polymer Poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate) (Poly(GMA-co-MTM)), which provides effective immobilization in the continuous flow system, was used in the sensor design. By taking the advantages of the nano-technology, carbon nanotubes (CNTs) were also added to the cell film and the sensitivity of the sensor was increased about 15 times. During the glycerol analysis in the continuous system, it was shown that composite film was not removed from the electrode surface and film elements were not washed out from the system. Glycerol analyses were performed by using batch loaded continuously flow cell at different flow rates of 1, 2, 4, and 6mL/min. The linear range was found as 2-100mM with the detection limit (LOD) of 0.057mM according to S/N=3. The calibration graphs were obtained for Poly(GMA-co-MTM)/G. oxydans and Poly(GMA-co-MTM)/CNT/G. oxydans biofilm electrodes in FIA mode, and sensitivities were found to be 1.50nA/mM and 19.13nA/mM, respectively. In this study, Poly(GMA-co-MTM) was used for the first time as a microbial matrix and was shown to be an effective immobilization agent.
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey. Electronic address: tulayergon@hotmail.com.
authors.forename: Tülay
authors.initials: T
authors.lastname: Ergön-Can
authors.affiliation: Department of Environmental Engineering, Gebze Technical University, 41400 Gebze, Kocaeli, Turkey. Electronic address: e.erhan@gtu.edu.tr.
authors.forename: Elif
authors.initials: E
authors.lastname: Erhan
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey. Electronic address: ofalgur@yahoo.com.
authors.forename: Ömer Faruk
authors.initials: ÖF
authors.lastname: Algur
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D020600:Gluconobacter oxydans / Q000378:metabolism*; D005990:Glycerol / Q000032:analysis*; D008567:Membranes, Artificial; D008768:Methylmethacrylates / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D008768:Methylmethacrylates; C549490:poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate); D005990:Glycerol
keywords: Carbon nanotube; Flow injection analysis; Gluconobacter oxydans; Glycerol; Microbial biosensor; Poly(GMA-co-MTM)