title: Legonaridin, a new member of linaridin RiPP from a Ghanaian Streptomyces isolate.
abstract: Linaridins are rare linear ribosomally-synthesized and post-translationally modified peptides (RiPPs) and only two, cypemycin and SGR-1832, in this family have been identified so far. Legonaridin 1 has been discovered as a new member of linaridins through chemical isolation, peptidogenomics, comprehensive 1- and 2-D NMR and advanced Marfey's analyses from the soil bacterium Streptomyces sp. CT34, an isolate collected from Legon, Ghana. Bioinformatics analysis of the gene cluster suggested that the biosynthesis of legonaridin 1 is different from those of cypemycin and SGR-1832. Consistent with bioinformatics and peptidogenomics analyses, 1 has a total of nine post-modifications, 8 dehydrobutyrine residues and a N,N-dimethylated N-terminus with a carboxylic acid at the C-terminus. Legonaridin 1 is structurally different from the two known linaridins comprising a new subfamily. This is the first time that NMR spectroscopy is used to establish the 2-D structure of a linaridin RiPP.
authors.affiliation: Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE Scotland, UK. h.deng@abdn.ac.uk m.jaspars@abdn.ac.uk.
authors.forename: Mostafa E
authors.initials: ME
authors.lastname: Rateb
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhai
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Ehrner
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Rath
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wang
authors.forename: Jioji
authors.initials: J
authors.lastname: Tabudravu
authors.forename: Rainer
authors.initials: R
authors.lastname: Ebel
authors.forename: Mervin
authors.initials: M
authors.lastname: Bibb
authors.forename: Kwaku
authors.initials: K
authors.lastname: Kyeremeh
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Dorrestein
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.forename: Hai
authors.initials: H
authors.lastname: Deng
mesh_terms: D000595:Amino Acid Sequence; D019295:Computational Biology; D057225:Data Mining; D008969:Molecular Sequence Data; D005810:Multigene Family; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012270:Ribosomes / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification
chemical_list: D010455:Peptides