title: Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection.
abstract: BACKGROUND
  Increasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.
  
  
  METHODS
  In this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques.
  
  
  RESULTS
  A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.
  
  
  CONCLUSIONS
  The determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields.
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. qianyucn@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. youhuaxiongcn@126.com.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. hanggaocn@126.com.
authors.forename: Hang
authors.initials: H
authors.lastname: Gao
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. jianliangliucn@126.com.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. zhiqiangchencn@126.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. yiwang1265@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. wendonglingcn@126.com.
authors.forename: Dongling
authors.initials: D
authors.lastname: Wen
mesh_terms: D000818:Animals; D019295:Computational Biology; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D020543:Proteome; D040901:Proteomics / Q000379:methods; D061987:Sf9 Cells; D015398:Signal Transduction; D018411:Spodoptera / Q000235:genetics / Q000378:metabolism / Q000821:virology*
chemical_list: D020543:Proteome