title: Actinoplanes bogoriensis sp. nov., a novel actinomycete isolated from leaf litter.
abstract: A novel actinomycete, designated LIPI11-2-Ac043(T), was isolated from leaf litter collected in Indonesia. According to phylogenetic analysis based on the 16S rRNA gene sequence comparisons, strain LIPI11-2-Ac043(T) was closely related to Actinoplanes abujensis A4029(T) (99.3%) and Actinoplanes brasiliensis DSM 43805(T) (98.8%). Spores of strain LIPI11-2-Ac043(T) were motile and the sporangia were spherical. The predominant menaquinone was MK-9(H6) and the principal polar lipids were phosphatidylinositol, phosphatidylethanolamine, phosphatidylglycerol and diphospatidylglycerol. The whole-cell sugars were galactose, glucose and mannose; rhibose, arabinose and xylose were also detected as minor components. The major fatty acids were anteiso-C15:0, iso-C16:0, iso-C15:0 and anteiso-C17:0. These data supported the affiliation of strain LIPI11-2-Ac043(T) to the genus Actinoplanes. Meanwhile, the results of DNA-DNA hybridization and physiological and biochemical tests indicated that strain LIPI11-2-Ac043(T) can be distinguished from its closest related species. Therefore, strain LIPI11-2-Ac043(T) represents a novel species of the genus Actinoplanes, for which the name Actinoplanes bogoriensis sp. nov. is proposed. The type strain is LIPI11-2-Ac043(T) (=InaCC A522(T)=NBRC 110975(T)).
authors.affiliation: Reserach Center for Biology, Indonesia Institute of Sciences (LIPI), Cibinong Science Center, West Java, Indonesia.
authors.forename: Arif
authors.initials: A
authors.lastname: Nurkanto
authors.affiliation: Reserach Center for Biotechnology, Indonesia Institute of Sciences (LIPI), Cibinong Science Center, West Java, Indonesia.
authors.forename: Puspita
authors.initials: P
authors.lastname: Lisdiyanti
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Reserach Center for Biotechnology, Indonesia Institute of Sciences (LIPI), Cibinong Science Center, West Java, Indonesia.
authors.forename: Shanti
authors.initials: S
authors.lastname: Ratnakomala
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibata
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007214:Indonesia; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S