title: Effect of ultraviolet-A radiation on the production of Leptolegnia chapmanii (Saprolegniales: Saprolegniaceae) zoospores on dead Aedes aegypti (Diptera: Culicidae) larvae and their larvicidal activity.
abstract: Impact of UV-radiation in entomopathogens in aquatic environments remains little investigated. The present study reports on the effect of UV-A on the larvicidal activity of Leptolegnia chapmanii zoospores in Aedes aegypti; on the production of zoospores in larvae killed by the pathogen and then exposed to UV-A; and on the activity of these zoospores against healthy larvae. Whereas the virulence of free zoospores in A. aegypti larvae was affected by a UV-A exposure time longer than 10min, production of zoospores in larvae and their virulence were not hampered at a maximal 8h exposure of dead larvae to UV-A. Findings suggest that dead larvae and zoosporangia provide a certain protection to zoospores against UV-A and emphasize the susceptibility of free encysted zoospores to such radiation.
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia (UFG), GO, Brazil; Centro de Estudios Parasitológicos y de Vectores (CEPAVE), Universidad Nacional de La Plata-CONICET, La Plata, Buenos Aires, Argentina.
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Rueda Páramo
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CEPAVE), Universidad Nacional de La Plata-CONICET, La Plata, Buenos Aires, Argentina.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: López Lastra
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CEPAVE), Universidad Nacional de La Plata-CONICET, La Plata, Buenos Aires, Argentina.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: García
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia (UFG), GO, Brazil.
authors.forename: Éverton K K
authors.initials: ÉK
authors.lastname: Fernandes
authors.affiliation: Faculdade de Farmácia, UFG, Goiânia, GO, Brazil.
authors.forename: Ricardo N
authors.initials: RN
authors.lastname: Marreto
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia (UFG), GO, Brazil. Electronic address: wchrisluz@hotmail.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Luz
mesh_terms: D000330:Aedes / Q000469:parasitology*; D000818:Animals; D007814:Larva / Q000528:radiation effects; D010572:Pest Control, Biological / Q000379:methods*; D044762:Saprolegnia / Q000472:pathogenicity* / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014774:Virulence
keywords: Entomopathogens; Mosquitoes; Oomycetes; UV-A radiation; Water molds