title: The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property.
abstract: Antarctic krill chitosan (A-Chitosan) was first evaluated in its hemostatic effect in this study. The prepared A-Chitosan powder showed low level of crystallinity and significantly high water binding capacity as 1293% (w/w). By mice tail amputation model and blood coagulation timing experiment, it is showed that this chitosan accelerated the tail hemostasis by 55% and shortened the blood clotting time by 38%. This efficacy was better than two other commercial chitosans investigated and was corresponding to their water binding capacities. Through examining the effect of chitosan on blood components, it could be found that platelets adhesion was mainly affected by the water binding capacity, and red blood cells aggregation was dependent on their deacetylation degree. The physicochemical properties resulted in better hydration property of chitosan would improve its hemostatic effect. These results suggested that Antarctic krill chitosan is a good candidate for hemostatic application.
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wu
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Zhuoyao
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Yue
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Department of Pharmacology, Harbin Medical University, Harbin 150081, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Pierre
authors.initials: P
authors.lastname: Ezanno
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Changshun
authors.initials: C
authors.lastname: Ruan
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China. Electronic address: zhaoxltju@gmail.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedic and Traumatology, The University of Hong Kong, 21 Sassoon Rd., Pokfulam, Hong Kong 999077, PR China.
authors.forename: William W
authors.initials: WW
authors.lastname: Lu
authors.affiliation: Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
authors.forename: Haobo
authors.initials: H
authors.lastname: Pan
mesh_terms: D000818:Animals; D001780:Blood Coagulation Tests; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D033201:Euphausiacea / Q000737:chemistry*; D006470:Hemorrhage / Q000188:drug therapy; D006490:Hemostatics / Q000737:chemistry* / Q000494:pharmacology*; D008810:Mice, Inbred C57BL; D015539:Platelet Activation / Q000187:drug effects*; D014867:Water / Q000737:chemistry
chemical_list: D006490:Hemostatics; D014867:Water; D048271:Chitosan
keywords: Antarctic krill; CaCl2 (PubChem CID:5284359); Calcein AM (PubChem CID:4126474); Chitosan; HCl (PubChem CID:313); Hemostatic agent; NaOH (PubChem CID:14798); acetic acid (PubChem CID:176); chloral hydrate (PubChem CID:2707); deuterium chloride (PubChem CID:522634); deuterium oxide (PubChem CID:24602); socium acetate (PubChem CID:517045); sodium citrate (PubChem CID:6224)