title: Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm system.
abstract: Urinary catheters expose patients to a high risk of acquiring nosocomial infections. To prevent this risk of infection, cellobiose dehydrogenase (CDH), an antimicrobial enzyme able to use various oligosaccharides as electron donors to produce hydrogen peroxide using oxygen as an electron acceptor, was covalently grafted onto plasma-activated urinary polydimethylsiloxane (PDMS) catheter surfaces. Successful immobilization of CDH on PDMS was confirmed by Fourier transformed-infrared spectrometry and production of H2 O2 . The CDH functionalized PDMS surfaces reduced the amount of viable Staphylococcus aureus by 60%, total biomass deposited on the surface by 30% and 70% of biofilm formation. The immobilized CDH was relatively stable in artificial urine over 16 days, retaining 20% of its initial activity. The CDH coated PDMS surface did not affect the growth and physiology of HEK 239 and RAW 264,7 mammalian cells. Therefore this new CDH functionalized catheter system shows great potential for solving the current problems associated with urinary catheters. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1448-1456, 2016.
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, 3430, Tulln, Austria. Barbara.Thallinger@boku.ac.at.
authors.forename: Barbara
authors.initials: B
authors.lastname: Thallinger
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, 3430, Tulln, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Brandauer
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, 3430, Tulln, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Burger
authors.affiliation: Department of Food Sciences and Technology, Food Biotechnology Laboratory, University of Natural Resources and Life Sciences, 1190, Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Sygmund
authors.affiliation: Department of Food Sciences and Technology, Food Biotechnology Laboratory, University of Natural Resources and Life Sciences, 1190, Vienna, Austria.
authors.forename: Roland
authors.initials: R
authors.lastname: Ludwig
authors.affiliation: Department d'Enginyeria Química, Universitat Politècnica de Catalunya, Grup de Biotecnologia Molecular i Industrial, 08222, Terrassa, Spain.
authors.forename: Kristina
authors.initials: K
authors.lastname: Ivanova
authors.affiliation: Synovo GesmbH, 72076, Tübingen, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Kun
authors.affiliation: Elettra Sincrotrone, 34149, Basovizza, TS, Italy.
authors.forename: Denis
authors.initials: D
authors.lastname: Scaini
authors.affiliation: Synovo GesmbH, 72076, Tübingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Burnet
authors.affiliation: Department d'Enginyeria Química, Universitat Politècnica de Catalunya, Grup de Biotecnologia Molecular i Industrial, 08222, Terrassa, Spain.
authors.forename: Tzanko
authors.initials: T
authors.lastname: Tzanov
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, 3430, Tulln, Austria. g.nyanhongo@boku.ac.at.|Botswana International University of Science and Technology, Private Mail Bag 16, Palapye Botswana. g.nyanhongo@boku.ac.at.
authors.forename: Gibson S
authors.initials: GS
authors.lastname: Nyanhongo
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, 3430, Tulln, Austria.
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000201:enzymology*; D018441:Biofilms / Q000254:growth & development*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D000067996:RAW 264.7 Cells; D013211:Staphylococcus aureus / Q000502:physiology*; D062885:Urinary Catheters
chemical_list: D004129:Dimethylpolysiloxanes; D005656:Fungal Proteins; C013830:baysilon; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase
keywords: antimicrobial effect; biocompatibility; cellobiose dehydrogenase; covalent immobilization; polydimethylsiloxane; urinary catheters