title: The role of the Kölliker-Fuse nuclei in the determination of abdominal motor output in a perfused brainstem preparation of juvenile rat.
abstract: The abdominal muscles are largely quiescent during normal breathing but may exhibit tonic activity or subtle respiratory modulation. The origin of baseline abdominal motor nerve activity (AbNA) if present remains uncharacterised. The contribution of the Kölliker-Fuse nucleus (KF) in the dorsolateral pons in the patterning and amplitude of AbNA was investigated using in situ perfused brainstem preparations of juvenile rats (n=12). Two types of AbNA were observed: Type I - expiratory-modulated (n=7), and Type II - weakly inspiratory/post-inspiratory-modulated (n=5). Despite this, all preparations exhibited the same bi-phasic late expiratory/postinspiratory bursts upon elicitation of the peripheral chemoreflex. Interestingly, the type of AbNA exhibited correlated with postinspiratory duration. Targeted microinjections of GABA-A receptor agonist isoguvacine (10mM; 70nl) into KF however did not significantly modify pattern or amplitude of baseline AbNA in either Type besides the selective abolition of the postinspiratory phase and, consequently, postinspiratory modulation in AbNAwhen present. In sum, the KF is not a major contributorin setting baseline abdominal motor output.
authors.affiliation: Systems Neurophysiology division, Florey Institute of Neuroscience and Mental Health, Gate 11 Royal Parade, University of Melbourne, VIC 3010, Australia. Electronic address: tara.bautista@florey.edu.au.
authors.forename: Tara G
authors.initials: TG
authors.lastname: Bautista
authors.affiliation: Systems Neurophysiology division, Florey Institute of Neuroscience and Mental Health, Gate 11 Royal Parade, University of Melbourne, VIC 3010, Australia.
authors.forename: Mathias
authors.initials: M
authors.lastname: Dutschmann
mesh_terms: D000005:Abdomen / Q000294:innervation / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D063226:Central Pattern Generators / Q000187:drug effects / Q000502:physiology; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000502:physiology; D058785:GABA-A Receptor Agonists / Q000494:pharmacology; D007539:Isonicotinic Acids / Q000494:pharmacology; D065836:Kolliker-Fuse Nucleus / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009068:Movement / Q000502:physiology*; D018373:Peripheral Nervous System Agents / Q000494:pharmacology; D010791:Phrenic Nerve / Q000502:physiology; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism; D012119:Respiration / Q000187:drug effects; D012132:Respiratory Muscles / Q000502:physiology; D012966:Sodium Cyanide / Q000494:pharmacology; D059246:Tachypnea / Q000503:physiopathology; D046509:Tissue Culture Techniques; D014630:Vagus Nerve / Q000502:physiology
chemical_list: D058785:GABA-A Receptor Agonists; D007539:Isonicotinic Acids; D018373:Peripheral Nervous System Agents; D011963:Receptors, GABA-A; D012966:Sodium Cyanide; C015300:isoguvacine
keywords: Abdominal; Brainstem; Central pattern generation; Control of breathing; Pons; Respiratory