title: Biofilm formation and ethanol inhibition by bacterial contaminants of biofuel fermentation.
abstract: Bacterial contaminants can inhibit ethanol production in biofuel fermentations, and even result in stuck fermentations. Contaminants may persist in production facilities by forming recalcitrant biofilms. A two-year longitudinal study was conducted of bacterial contaminants from a Midwestern dry grind corn fuel ethanol facility. Among eight sites sampled in the facility, the combined liquefaction stream and yeast propagation tank were consistently contaminated, leading to contamination of early fermentation tanks. Among 768 contaminants isolated, 92% were identified as Lactobacillus sp., with the most abundant species being Lactobacillus plantarum, Lactobacillus casei, Lactobacillus mucosae, and Lactobacillus fermentum. Seven percent of total isolates showed the ability to form biofilms in pure cultures, and 22% showed the capacity to significantly inhibit ethanol production. However, these traits were not correlated. Ethanol inhibition appeared to be related to acetic acid production by contaminants, particularly by obligately heterofermentative species such as L. fermentum and L. mucosae.
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA(1).
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Rich
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA(1). Electronic address: tim.leathers@ars.usda.gov.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Leathers
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA(1).
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Bischoff
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA(1).
authors.forename: Amber M
authors.initials: AM
authors.lastname: Anderson
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA(1).
authors.forename: Melinda S
authors.initials: MS
authors.lastname: Nunnally
mesh_terms: D018441:Biofilms; D056804:Biofuels / Q000382:microbiology*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D000431:Ethanol; D005285:Fermentation; D007778:Lactobacillus / Q000502:physiology; D008137:Longitudinal Studies; D015003:Yeasts; D003313:Zea mays / Q000382:microbiology
chemical_list: D056804:Biofuels; D000431:Ethanol
keywords: Biofilm; Biofuel; Contaminants; Inhibition of ethanol production; Lactobacillus