title: Quantitative Assessment of Iron-Labeled Stem-Cell Adhesion at the Vessel Wall in a Vascular Flow Model: Correlation of T2*-Weighted Imaging at 3 T and Histology.
abstract: OBJECTIVE
  To evaluate the distribution of superparamagnetic iron oxide (SPIO)-labeled cells in a perfused segment of a porcine artery and to estimate the number of adherent cells by means of magnetic resonance (MR) imaging.
  
  
  METHODS
  Six vessel specimens (diameters between 0.8 and 1.2 cm) were placed in a bioreactor system, and 2 × 10(4) to 1 × 10(6) SPIO-labeled endothelial colony-forming cells were injected into the artery within the perfused reactor. The area of resulting signal extinctions at the inner wall of the vessels was quantified on MR images by using a high-resolution T2*-weighted sequence with a slice-by-slice approach. After imaging, the labeled cells were quantified histologically.
  
  
  RESULTS
  The total iron load of each cell was 56.5 pg ± 14.4. In the applied range of 2 × 10(4) to 1 × 10(6) cells per vessel, the area of iron-induced signal extinction at the vessel wall on T2*-weighted imaging corresponded to the histologically detected cell number (r = 0.98, P < .001).
  
  
  CONCLUSIONS
  A correlation between the area of signal extinction and the number of labeled cells at the vessel wall was found. This might help to evaluate dose rates in further clinical applications of intravascular cell-based therapies.
authors.affiliation: Division of Diagnostic Radiology, Department of Diagnostic and Interventional Radiology, Section of Experimental Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany. Electronic address: gerd.groezinger@med.uni-tuebingen.de.
authors.forename: Gerd
authors.initials: G
authors.lastname: Grözinger
authors.affiliation: Department of Diagnostic and Interventional Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Fritz
authors.initials: F
authors.lastname: Schick
authors.affiliation: Department of Diagnostic and Interventional Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Eibofner
authors.affiliation: Division of Diagnostic Radiology, Department of Diagnostic and Interventional Radiology, Section of Experimental Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wiesinger
authors.affiliation: Department of General, Visceral and Transplant Surgery, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Schenk
authors.affiliation: Division of Diagnostic Radiology, Department of Diagnostic and Interventional Radiology, Section of Experimental Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Grosse
authors.affiliation: Department of Thoracic, Cardiac, and Vascular Surgery, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Wendel
authors.affiliation: Division of Diagnostic Radiology, Department of Diagnostic and Interventional Radiology, Section of Experimental Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Elser
authors.affiliation: Division of Diagnostic Radiology, Department of Diagnostic and Interventional Radiology, Section of Experimental Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Nikolaou
authors.affiliation: Division of Diagnostic Radiology, Department of Diagnostic and Interventional Radiology, Section of Experimental Radiology, University of Tübingen, Hoppe-Seyler-Str. 3, D-72076 Tübingen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schmehl
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media; D003911:Dextrans; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D053783:Magnetic Resonance Imaging, Interventional / Q000379:methods*; D058185:Magnetite Nanoparticles; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013223:Statistics as Topic; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells; D013552:Swine; D013895:Thoracic Arteries / Q000166:cytology* / Q000502:physiology* / Q000601:surgery
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10