title: Proline-Rich Peptide Mimics Effects of Enamel Matrix Derivative on Rat Oral Mucosa Incisional Wound Healing.
abstract: BACKGROUND
  Proline-rich peptides have been shown to promote periodontal regeneration. However, their effect on soft tissue wound healing has not yet been investigated. The aim of this study is to evaluate the effect of enamel matrix derivative (EMD), tyrosine-rich amelogenin peptide (TRAP), and a synthetic proline-rich peptide (P2) on acute wound healing after a full-thickness flap procedure in an incisional rat model.
  
  
  METHODS
  This experimental study has a split-mouth, randomized, placebo-controlled design. Test and control wounds were created on the palatal mucosa of 54 Sprague-Dawley rats. Wounds were histologically processed, and reepithelialization, leukocyte infiltration, and angiogenesis were assessed at days 1, 3, and 7 post-surgery.
  
  
  RESULTS
  EMD and P2 significantly promoted early wound closure at day 1 (P <0.001 and P = 0.004, respectively). EMD maintained a significant acceleration of reepithelialization at day 3 (P = 0.004). Wounds treated by EMD and P2 showed increased angiogenesis during the first 3 days of healing (P = 0.03 and 0.001, respectively). Leukocyte infiltration was decreased in EMD-treated wounds at day 1 (P = 0.03), and P2 and TRAP induced a similar effect at days 3 (P = 0.002 and P <0.0001, respectively) and 7 (P = 0.005 and P <0.001).
  
  
  CONCLUSIONS
  EMD and P2 promoted reepithelialization and neovascularization in full-thickness surgical wounds on rat oral mucosa.
authors.affiliation: Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
authors.forename: Oscar
authors.initials: O
authors.lastname: Villa
authors.affiliation: Department of Periodontology, Institute of Clinical Dentistry, University of Oslo.
authors.forename: Johan C
authors.initials: JC
authors.lastname: Wohlfahrt
authors.affiliation: Department of Oral Biology, University of Oslo.
authors.forename: Ibrahimu
authors.initials: I
authors.lastname: Mdla
authors.affiliation: Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
authors.forename: Christiane
authors.initials: C
authors.lastname: Petzold
authors.affiliation: Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
authors.forename: Janne E
authors.initials: JE
authors.lastname: Reseland
authors.affiliation: Herman Ostrow School of Dentistry, Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA.
authors.forename: Malcolm L
authors.initials: ML
authors.lastname: Snead
authors.affiliation: Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
authors.forename: Staale P
authors.initials: SP
authors.lastname: Lyngstadaas
mesh_terms: D000818:Animals; D003743:Dental Enamel; D003746:Dental Enamel Proteins; D009061:Mouth Mucosa; D010455:Peptides; D011392:Proline; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D003746:Dental Enamel Proteins; D010455:Peptides; D011392:Proline
keywords: Angiogenesis inducing agents; dental enamel proteins; guided periodontal tissue regeneration; mouth mucosa; proline-rich protein domains; wound healing