title: Increased Butyrate Production During Long-Term Fermentation of In Vitro-Digested High Amylose Cornstarch Residues with Human Feces.
abstract: An in vitro semi-continuous long-term (3 wk) anaerobic incubation system simulating lower gut fermentation was used to determine variability in gut microbial metabolism between 4 predigested high amylose-resistant starch residues (SR): SRV, SRVI, SRVII, and SRGEMS in human fecal samples. Subjects participated twice, 5 mo apart: 30 in Phase I (15 lean, 9 overweight and 6 obese), 29 in Phase II (15 lean, 9 overweight, 5 obese); 13 of 15 lean subjects participated in both phases. Of the 4 SRs, SRV displayed the highest gelatinization temperature, peak temperature, enthalpy changes, and the least digestibility compared with the other SRs. In both phases, compared with blank controls, all SRs increased butyrate ∼2-fold which stabilized at week 2 and only SRV caused greater propionate concentration (∼30%) after 3 wk which might have been partly mediated by its lesser digestibility. Fecal samples from lean and overweight/obese subjects incubated with SRs showed similar short-chain fatty acid production across both time points, which suggests that resistant starch may benefit individuals across BMIs.
authors.affiliation: Dept. of Food Science and Human Nutrition, Iowa State Univ, 220 Mackay Hall, Ames, IA, 50011, U.S.A.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Dept. of Food Science and Human Nutrition, Iowa State Univ, 220 Mackay Hall, Ames, IA, 50011, U.S.A.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Dept. of Food Science and Human Nutrition, Iowa State Univ, 220 Mackay Hall, Ames, IA, 50011, U.S.A.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Dept. of Statistics, Iowa State Univ, 1121 Snedecor Hall, Ames, IA, 50011, U.S.A.
authors.forename: Man-Yu
authors.initials: MY
authors.lastname: Yum
authors.affiliation: Dept. of Agriculture Sciences, TrumanState Univ, 2072 Magruder Hall, Kirksville, MO, 63501, U.S.A.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Campbell
authors.affiliation: Dept. of Food Science and Human Nutrition, Iowa State Univ, 220 Mackay Hall, Ames, IA, 50011, U.S.A.
authors.forename: Jay-Lin
authors.initials: JL
authors.lastname: Jane
authors.affiliation: Dept. of Food Science and Human Nutrition, Iowa State Univ, 220 Mackay Hall, Ames, IA, 50011, U.S.A.
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: White
authors.affiliation: Dept. of Food Science and Human Nutrition, Iowa State Univ, 220 Mackay Hall, Ames, IA, 50011, U.S.A.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Hendrich
mesh_terms: D000328:Adult; D000688:Amylose / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism; D015992:Body Mass Index; D020148:Butyric Acid / Q000378:metabolism*; D004032:Diet; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004063:Digestion; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D000069196:Gastrointestinal Microbiome; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008954:Models, Biological; D009765:Obesity / Q000378:metabolism / Q000382:microbiology; D011422:Propionates / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
chemical_list: D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D011422:Propionates; D020148:Butyric Acid; D013213:Starch; D000688:Amylose
keywords: BMI; butyrate; gut microbiota; resistant starch; short chain fatty acid