title: A New Fluorescence Turn-On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging.
abstract: The development of novel selective probes with high sensitivity for the detection of Al(3+) is widely considered an important research goal due to the importance of such probes in medicine, living systems and the environment. Here, we describe a new fluorescent probe, N'-(4-diethylamino-2-hydroxybenzylidene)-2-hydroxybenzohydrazide (1), for Al(3+). Probe 1 was evaluated in a solution of acetonitrile/water (1:1 v/v). Compared with previously reported probes for Al(3+), probe 1 can be synthesized easily and in high yield. A Job plot confirmed that probe 1 is able to complex Al(3+) in a 1:1 ratio, and the binding constant was determined to be 4.25×10(8) m(-1). Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity. Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe. Finally, the ability of probe 1 to act as a fluorescent probe for Al(3+) in living systems was evaluated in Gram-negative bacteria, Escherichia coli, and confocal laser scanning microscopy confirmed its utility. The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.
authors.forename: Lei-Li
authors.initials: LL
authors.lastname: Sun
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.
authors.forename: Guang-Zong
authors.initials: GZ
authors.lastname: Ren
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Niu
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.
authors.forename: Wen-Zhou
authors.initials: WZ
authors.lastname: Hu
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Hu
keywords: aluminum(III); bioimaging; fluorescent probes; selectivity; sensitivity