title: [The Radiosensitizing Effect of Resveratrol on Hopypharyngeal Carcinoma Cell Line FADU and its Effect on the Cell Cycle].
abstract: OBJECTIVE
  To study the radiosensitizing effect of resveratrol on hypopharyngeal carcinoma cell line FADU in vitro.
  
  
  METHODS
  Hypopharyngeal carcinoma cell line FADU was cultured in in vitro DMEM. Its inhibition on cell proliferation was detected using cytotoxicity test (MTT assay). The cell survival curve was drawn using clone formation to obtain sensitive enhancement ratio (SER). Changes of the cell cycle and cell apoptosis were analyzed using flow cytometry (FCM).
  
  
  RESULTS
  Results of MTT showed the inhibition of resveratrol on FADU cells increased along with its concentrations (P < 0.05). Results of clone formation indicated the surviving fraction at 2 Gy (SF2) was 0.717 ± 0.062 in the irradiation group, and 0.426 ± 0.035 in the resveratrol plus irradiation group (with SER ranged 1.684 ± 0.178) with statistical difference (P = 0.007). Results of FCM showed that after radiation of 4 Gy radiation, cells at G2/M phase arrest increased, but cells at G1 decreased. After radiation of resveratrol for 24 h, cells at G1 decreased, but cells at G2/M phase and S phase arrest increased. When 4 Gy radiation combined resveratrol was used, cells at G2/M phase arrest significantly increased, but cells at G1 significantly decreased. The apoptosis rate was 1.94% ± 1.65% in the control group, 4.56% ± 0.92% in the irradiation group, 2.03% ± 1.46% in the resveratrol group, and 23.11% ± 7.22% in the resveratrol plus irradiation group. There was statistical difference between the resveratrol plus irradiation group and the rest 3 groups (P < 0.05).
  
  
  CONCLUSIONS
  Resveratrol could enhance the radiosensitivity of hypopharyngeal carcinoma FADU cells in vitro possibly by inducing cell apoptosis and causing changes in the cell cycle distribution.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shao
authors.forename: Fang
authors.initials: F
authors.lastname: Quan
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Li
authors.forename: Xiao-bao
authors.initials: XB
authors.lastname: Yao
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Rui-min
authors.initials: RM
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D006258:Head and Neck Neoplasms; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000188:drug therapy; D011836:Radiation Tolerance; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*; D000077185:Resveratrol; D000077195:Squamous Cell Carcinoma of Head and Neck; D013267:Stilbenes / Q000627:therapeutic use*
chemical_list: D011838:Radiation-Sensitizing Agents; D013267:Stilbenes; D000077185:Resveratrol