title: Matrine and oxymatrine in corroborant plant extracts and fertilizers: HPLC/MS-MS method development and single-laboratory validation.
abstract: A reversed phase high-performance liquid chromatographic method (HPLC/MS-MS) has been developed and validated for detection of alkaloids matrine and oxymatrine in fertilizer with labeled enhancer plant defense activities. The analytical method was validated statistically. The results show a strong matrix effect, requiring quantification by standard addition method. The regression lines showed r(2) > 0.994. Recoveries ranging from 97 to 104% were obtained for the fortification level of 0.01% wt wt(-1) and the relative standard deviations ranged from 3 to 4% (n = 10). The limits of detection were below 0.0001% wt wt(-1), while the limits of quantification did not exceed 0.0004% wt wt(-1). The method is currently applied in ICQRF Laboratory of Catania on fertilized and corroborant plant extract collected in the Italian market in the frame of MIPAAF institutional quality control activity, with the aim to dectect these unpermitted active substances.
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sabatino
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Scarangella
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Lazzaro
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Scordino
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Giavanna
authors.initials: G
authors.lastname: Picariello
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Leotta
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Pasqualino
authors.initials: P
authors.lastname: Traulo
authors.affiliation: a Italian Ministry of Agriculture, Foodstuff and Forestry Policies (MIPAAF), Department of Central Inspectorate for Quality Safeguarding and Fraud Repressing of Agricultural and Foodstuff Products (ICQRF), Laboratory of Catania , Catania , Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Gagliano
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005308:Fertilizers / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D011807:Quinolizines / Q000032:analysis*; D029909:Sophora / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D005308:Fertilizers; D010936:Plant Extracts; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines
keywords: Alkaloids; HPLC/MS-MS; Sophora; bio-pesticide; plant protection product