title: Involvement of Ethylene in the Latex Metabolism and Tapping Panel Dryness of Hevea brasiliensis.
abstract: Ethephon, an ethylene releaser, is used to stimulate latex production in Hevea brasiliensis. Ethylene induces many functions in latex cells including the production of reactive oxygen species (ROS). The accumulation of ROS is responsible for the coagulation of rubber particles in latex cells, resulting in the partial or complete stoppage of latex flow. This study set out to assess biochemical and histological changes as well as changes in gene expression in latex and phloem tissues from trees grown under various harvesting systems. The Tapping Panel Dryness (TPD) susceptibility of Hevea clones was found to be related to some biochemical parameters, such as low sucrose and high inorganic phosphorus contents. A high tapping frequency and ethephon stimulation induced early TPD occurrence in a high latex metabolism clone and late occurrence in a low latex metabolism clone. TPD-affected trees had smaller number of laticifer vessels compared to healthy trees, suggesting a modification of cambial activity. The differential transcript abundance was observed for twenty-seven candidate genes related to TPD occurrence in latex and phloem tissues for ROS-scavenging, ethylene biosynthesis and signalling genes. The predicted function for some Ethylene Response Factor genes suggested that these candidate genes should play an important role in regulating susceptibility to TPD.
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. rizaputranto@iribb.org.|Indonesian Research Institute for Biotechnology and Bioindustry, Bogor 16128, Indonesia. rizaputranto@iribb.org.
authors.forename: Riza-Arief
authors.initials: RA
authors.lastname: Putranto
authors.affiliation: Indonesia Rubber Research Institute, Sembawa Research Centre, Palembang 30001, Indonesia. eva_herlinawati@yahoo.com.
authors.forename: Eva
authors.initials: E
authors.lastname: Herlinawati
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. maryannick.rio@cirad.fr.
authors.forename: Maryannick
authors.initials: M
authors.lastname: Rio
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. julie.leclercq@cirad.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Leclercq
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. nuch1505@yahoo.com.|Rubber Research Institute of Thailand, Chatuchak, Bangkok 10900, Thailand. nuch1505@yahoo.com.
authors.forename: Piyanuch
authors.initials: P
authors.lastname: Piyatrakul
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité de Recherche Performance des Systèmes de Culture des Plantes Pérennes, F-34398 Montpellier, France. eric.gohet@cirad.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Gohet
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. christine.sanier@cirad.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Sanier
authors.affiliation: Indonesia Rubber Research Institute, Sembawa Research Centre, Palembang 30001, Indonesia. fetrina_oktavia@yahoo.com.
authors.forename: Fetrina
authors.initials: F
authors.lastname: Oktavia
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. julien_pirrello@yahoo.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Pirrello
authors.affiliation: Indonesia Rubber Research Institute, Bogor 16151, Indonesia. kuswhd@yahoo.com.
authors.lastname: Kuswanhadi
authors.affiliation: Centre International de Recherche Agronomique pour le Développement, Unité Mixte de Recherche Amélioration Génétique & Adaptation des Plantes Méditerranéennes et Tropicales, F-34398 Montpellier, France. pascal.montoro@cirad.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Montoro
mesh_terms: D005030:Ethylenes / Q000378:metabolism*; D028482:Hevea / Q000235:genetics / Q000378:metabolism*; D007840:Latex / Q000096:biosynthesis* / Q000378:metabolism; D052585:Phloem / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010935:Plant Diseases; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D013395:Sucrose / Q000378:metabolism; D059467:Transcriptome
chemical_list: D005030:Ethylenes; D007840:Latex; D010710:Phosphates; D017382:Reactive Oxygen Species; D013395:Sucrose; C036216:ethylene
keywords: abiotic stress; antioxidant; ethephon; ethylene response factor; oxidative stress; rubber; transcription factor