title: Pseudomonas deceptionensis DC5-mediated synthesis of extracellular silver nanoparticles.
abstract: The biological synthesis of metal nanoparticles is of great interest in the field of nanotechnology. The present work highlights the extracellular biological synthesis of silver nanoparticles using Pseudomonas deceptionensis DC5. The particles were synthesized in the culture supernatant within 48 h of incubation. Extracellular synthesis of silver nanoparticles in the culture supernatant was confirmed by ultraviolet-visible spectroscopy, which showed the absorption peak at 428 nm, and also under field emission transmission electron microscopy which displayed the spherical shape. In addition, the particles were characterized by X-ray diffraction spectroscopy, which corresponds to the crystalline nature of nanoparticles, and energy-dispersive X-ray analysis which exhibited the intense peak at 3 keV, resembling the silver nanoparticles. Further, the synthesized nanoparticles were examined by elemental mapping which displayed the dominance of the silver element in the synthesized product, and dynamic light scattering which showed the distribution of silver nanoparticles with respect to intensity, volume, and number of particles. Moreover, the silver nanoparticles have been found to be quite active in antimicrobial activity and biofilm inhibition activity against pathogenic microorganisms. Thus, the present work emphasized the prospect of using the P. deceptionensis DC5 to achieve the extracellular synthesis of silver nanoparticles in a facile and environmental manner.
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Jae H
authors.initials: JH
authors.lastname: Jo
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Yeon J
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: b Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University , Republic of Korea.
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.
authors.forename: Chi-Gyu
authors.initials: CG
authors.lastname: Jin
authors.affiliation: a Department of Oriental Medicine Biotechnology and Ginseng Bank , College of Life Sciences, Kyung Hee University , Yongin , Republic of Korea.|b Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University , Republic of Korea.
authors.forename: Deok C
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011549:Pseudomonas / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: anti-biofilm; antimicrobial activity; biogenic; extracellular; silver nanoparticles