title: Surgery-Induced Weight Loss Is Associated With the Downregulation of Genes Targeted by MicroRNAs in Adipose Tissue.
abstract: BACKGROUND
  Molecular mechanisms associated with physiological variations in adipose tissue (AT) are not fully recognized. The most recent reports highlight the critical relevance of microRNAs (miRNAs) found in AT.
  
  
  OBJECTIVE
  To identify changes in messenger RNA (mRNA) and miRNA expressions and their interaction in human AT before and after surgery-induced weight loss. Research Design and Setting: Genome-wide mRNA and miRNA expressions were assessed by microarrays in abdominal subcutaneous AT of 16 morbidly obese women before and 2 years after laparoscopic Roux-en-Y gastric bypass. The association of changes in miRNAs with their respective mRNA targets was studied. The results were replicated in publicly available microarray datasets. Validation was made by real-time polymerase chain reaction in additional fat samples from 26 age-matched lean women and in isolated human adipocytes.
  
  
  RESULTS
  A total of 5018 different mRNA probe sets and 15 miRNAs were differentially expressed after surgery-induced weight loss. The clustering of similar expression patterns for gene products with related functions revealed molecular footprints that elucidate significant changes in cell cycle, development, lipid metabolism, and the inflammatory response. The participation of inflammation was demonstrated by results assessed in isolated adipocytes. Interestingly, when transcriptomes were analyzed taking into account the presence of miRNA target sites, miRNA target mRNAs were upregulated in obese AT (P value = 2 × 10(-181)) and inflamed adipocytes (P value = 4 × 10(-61)), according to the number of target sites harbored by each transcript.
  
  
  CONCLUSIONS
  Current findings suggest impaired miRNA target gene expression in obese AT in close association with inflammation, both improving after weight loss.
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Ortega
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Mercader
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Moreno-Navarrete
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: Nonell
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Eulàlia
authors.initials: E
authors.lastname: Puigdecanet
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: José I
authors.initials: JI
authors.lastname: Rodriquez-Hermosa
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Rovira
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Xifra
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Ester
authors.initials: E
authors.lastname: Guerra
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Moreno
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Mayas
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Moreno-Castellanos
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Fernández-Formoso
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Wifredo
authors.initials: W
authors.lastname: Ricart
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Tinahones
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Torrents
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: María M
authors.initials: MM
authors.lastname: Malagón
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition (F.J.O., J.M.M.-N., O.R., G.X., E.G., M.M., W.R., J.M.R.-R.), Institut d'Investigació Biomédica de Girona, Girona, Spain; CIBER de la Fisiopatología de la Obesidad y la Nutrición and Instituto de Salud Carlos III (F.J.O., J.M.M.-N., O.R., D.M., N.M.-C., J.A.F.-F., W.R., F.J.T., M.M.M., J.M.F.-R.), Spain; Joint BSC-CRG-IRB Program on Computational Biology (J.M.M., D.T.), Barcelona Supercomputing Center, Barcelona, Spain; Servei d'Anàlisi de Microarrays (L.N., E.P.), Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain; Department of Surgery (J.I.R.-H.), Institut d'Investigació Biomédica de Girona, Girona, Spain; Service of Endocrinology and Nutrition (D.M., F.J.T.), Hospital Clínico Universitario Virgen de Victoria de Malaga, Málaga, Spain; Department of Cell Biology, Physiology and Immunology (N.M.-C., M.M.M.), Instituto Maimonides de Investigaciones Biomedicas de Cordoba/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; and Institució Catalana de Recerca i Estudis Avançats (D.T.), Barcelona, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Fernández-Real
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D000328:Adult; D015992:Body Mass Index; D002460:Cell Line; D002478:Cells, Cultured; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D015536:Down-Regulation; D005260:Female; D015390:Gastric Bypass; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006801:Humans; D008137:Longitudinal Studies; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009767:Obesity, Morbid / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000601:surgery*; D012333:RNA, Messenger / Q000378:metabolism; D050507:Subcutaneous Fat, Abdominal / Q000276:immunology / Q000378:metabolism*; D015431:Weight Loss
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger