title: Characterization of Hyaluronan-Degrading Enzymes from Yeasts.
abstract: Hyaluronidases (HAases) from yeasts were characterized for the first time. The study elucidated that hyaluronate 4-glycanohydrolase and hyaluronan (HA) lyase can be produced by yeasts. Six yeasts producing HAases were found through express screening of activities. The extracellular HAases from two of the yeast isolates, Pseudozyma aphidis and Cryptococcus laurentii, were characterized among them. P. aphidis HAase hydrolyzed β-1,4 glycosidic bonds of HA, yielding even-numbered oligosaccharides with N-acetyl-D-glucosamine at the reducing end. C. laurentii produced hyaluronan lyase, which cleaved β-1,4 glycosidic bonds of HA in β-elimination reaction, and the products of HA degradation were different-sized even-numbered oligosaccharides. The shortest detected HA oligomer was dimer. The enzymes' pH and temperature optima were pH 3.0 and 37-45 °C (P. aphidis) and pH 6.0 and 37 °C (C. laurentii), respectively. Both HAases showed good thermostability.
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Dzianis.Smirnou@contipro.com.
authors.forename: Dzianis
authors.initials: D
authors.lastname: Smirnou
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Martin.Krcmar@contipro.com.
authors.forename: Martin
authors.initials: M
authors.lastname: Krčmář
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Jaromir.Kulhanek@contipro.com.
authors.forename: Jaromír
authors.initials: J
authors.lastname: Kulhánek
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Martina.Hermannova@contipro.com.
authors.forename: Martina
authors.initials: M
authors.lastname: Hermannová
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Lenka.Bobkova@contipro.com.
authors.forename: Lenka
authors.initials: L
authors.lastname: Bobková
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Lukas.Franke@contipro.com.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Franke
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Stanislav.Pepeliaev@contipro.com.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Pepeliaev
authors.affiliation: Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Vladimir.Velebny@contipro.com.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
mesh_terms: D005110:Extracellular Space / Q000201:enzymology; D006820:Hyaluronic Acid / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015003:Yeasts / Q000166:cytology / Q000201:enzymology*
chemical_list: D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase
keywords: Characteristics; Cryptococcus laurentii; Hyaluronate 4-glycanohydrolase; Lyase; Pseudozyma aphidis; Thermostability