title: Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification.
abstract: We develop a sensitive fluorescence method for DNA methyltransferase (MTase) assay based on T7 RNA polymerase-mediated transcription amplification and duplex-specific nuclease (DSN)-assisted cyclic signal amplification. This method exhibits excellent specificity and high sensitivity with a detection limit of 0.015 U mL(-1), and it may be further applied for the screening of antimicrobial drugs.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Xu
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Zeng
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D005453:Fluorescence; D005839:Gentamicins / Q000737:chemistry; D057230:Limit of Detection; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000037:antagonists & inhibitors / Q000737:chemistry*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D014764:Viral Proteins; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases