title: Axud1 Integrates Wnt Signaling and Transcriptional Inputs to Drive Neural Crest Formation.
abstract: Neural crest cells are induced at the neural plate border by the combined action of transcription factors and signaling molecules. Here, we show that Axud1, a downstream effector of Wnt signaling, represents a critical missing link that integrates signaling and transcriptional cues to mediate neural crest formation. Axud1 is a transcription factor expressed in neural crest progenitors in a Wnt1/β-catenin-dependent manner. Axud1 loss leads to downregulation of multiple genes involved in neural crest specification, similar to the effects of Wnt1 knockdown. Importantly, Axud1 is sufficient to rescue neural crest formation after disruption of Wnt signaling. Furthermore, it physically interacts with neural plate border genes Pax7 and Msx1 in vivo to directly activate transcription of stem cell factor FoxD3, initiating the neural crest program. Thus, Axud1 integrates Wnt signaling with transcriptional inputs to endow the neural crest with its unique molecular signature.
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Marcos
authors.initials: M
authors.lastname: Simões-Costa
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Stone
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: mbronner@caltech.edu.
authors.forename: Marianne E
authors.initials: ME
authors.lastname: Bronner
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D030161:Avian Proteins / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology; D002642:Chick Embryo; D002645:Chickens; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009432:Neural Crest / Q000378:metabolism*; D054258:Neural Plate / Q000378:metabolism; D014158:Transcription, Genetic; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D030161:Avian Proteins; D051153:Wnt Proteins; D051176:beta Catenin