title: Putative carotenoid genes expressed under the regulation of Shine-Dalgarno regions in Escherichia coli for efficient lycopene production.
abstract: OBJECTIVE
  Putative genes crtE, crtB, and crtI from Deinococcus wulumiqiensis R12, a novel species, were identified by genome mining and were co-expressed using the optimized Shine-Dalgarno (SD) regions to improve lycopene yield.
  
  
  RESULTS
  A lycopene biosynthesis pathway was constructed by co-expressing these three genes in Escherichia coli. After optimizing the upstream SD regions and the culture medium, the recombinant strain EDW11 produced 88 mg lycopene g(-1) dry cell wt (780 mg lycopene l(-1)) after 40 h fermentation without IPTG induction, while the strain EDW without optimized SD regions only produced 49 mg lycopene g(-1) dry cell wt (417 mg lycopene l(-1)).
  
  
  CONCLUSIONS
  Based on the optimization of the upstream SD regions and culture medium, the yield of the strain EDW11 reached a high level during microbial lycopene production until now.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. aileenfish2013@njtech.edu.cn.
authors.forename: Weiyue
authors.initials: W
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. xuxian@njtech.edu.cn.
authors.forename: Xian
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Food Science and Light Industry, Nanjing Tech University, Nanjing, People's Republic of China. jiangling@njtech.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, Xinjiang Uigur Autonomous Region, People's Republic of China. zhangzheedong@sohu.com.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. lishuang@njtech.edu.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. biotech@njtech.edu.cn.|School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, People's Republic of China. biotech@njtech.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002338:Carotenoids / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D051231:Farnesyltranstransferase / Q000235:genetics* / Q000378:metabolism; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000235:genetics* / Q000378:metabolism; D000077276:Lycopene; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002338:Carotenoids; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; D051231:Farnesyltranstransferase; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D000077276:Lycopene
keywords: Carotenoid genes; Deinococcus wulumiqiensis R12; Escherichia coli; Lycopene; Regulation; Shine-Dalgarno regions