title: Renal interstitial mast cell count is significantly higher in membranoproliferative glomerulonephritis than in class IV lupus nephritis.
abstract: Lupus nephritis (LN) is a severe manifestation of systemic lupus erythematosus; in LN class IV morphologic lesions may be similar to the lesions in primary membranoproliferative glomerulonephritis (MPGN). The aim of the study was to compare the counts of tryptase-positive and chymase-positive mast cells between LN class IV and MPGN. The material consisted of 61 renal biopsies: 32 with lupus nephritis class IV, and 29 with membranoproliferative glomerulonephritis. Chymase- and tryptase-positive cells were stained by immunohistochemistry and subsequently counted. The mean count of chymase-positive mast cells was 21.94 for the whole group, 12.66 for LN class IV and 32.18 for MPGN. The mean count of tryptase-positive cells was 34.94 hpf for the entire group, 22.98 for LN class IV and 48. 13 for MPGN. The differences between lupus nephritis and membranoproliferative glomerulonephritis were significant both for chymase- and tryptase-positive cells. Both chymase-positive MC counts and tryptase-positive MC counts correlated with relative interstitial volume (RIV) (R=0.35 and R=0.28, respectively) and with creatinine level (R=0.35 and R=0.43, respectively). There was also a significant correlation between age, creatinine level and RIV (R=0.28 and R=0.26, respectively).
authors.forename: Karolina
authors.initials: K
authors.lastname: Kaczmarczyk
authors.forename: Jacek
authors.initials: J
authors.lastname: Musiał
authors.forename: Jerzy
authors.initials: J
authors.lastname: Soja
authors.forename: Marek
authors.initials: M
authors.lastname: Kuźniewski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gala-Błądzińska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Białas
authors.affiliation: Krzysztof Okoń MD PhD, Department of Pathomorphology, Jagiellonian University Collegium Medicum, Grzegórzecka 16, 31-531 Krakow, Poland, e-mail: mpokon@cyf-kr.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Okoń
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D002452:Cell Count; D053818:Chymases / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D015432:Glomerulonephritis, Membranoproliferative / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008181:Lupus Nephritis / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology*; D008875:Middle Aged; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D053802:Tryptases / Q000032:analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D053818:Chymases; D053802:Tryptases