title: Involvement of galanin receptor 2 and CaMKII in galanin-induced antinociception in periaqueductal grey of rats.
abstract: The present study was performed to explore the effect of the galanin receptor 2 (GalR2) antagonist M871 on the galanin-induced antinociception in periaqueductal grey (PAG), and an involvement of Ca(2+)/calmodulin-dependent kinase II (CaMKII) in the galanin-induced antinociception. Intra-PAG injection of galanin induced marked increases in HWLs to noxious thermal and mechanical stimulation. The increased HWLs to thermal and mechanical stimulation decreased significantly after intra-PAG administration of the GalR2 antagonist M871, indicating an involvement of GalR2 in the galanin-induced antinociception in PAG of rats. Furthermore, rats received intra-PAG injection of galanin, followed 5min later by intra-PAG administration of the CaMKII inhibitor MAP. The galanin-induced increases in HWLs to thermal and mechanical stimulation decreased significantly after intra-PAG administration of MAP, indicating that there is an involvement of CaMKII in the galanin-induced antinociception in PAG, blockade the activity of CaMKII by MAP inhibits the galanin-induced antinociception in PAG of rats. Our results strongly indicate that the galanin-induced antinociception is mediated by GalR2 in the PAG, and CaMKII may be involved in the galanin-induced antinociception in PAG of rats.
authors.affiliation: Laboratory of Neuropharmacology, School of Pharmacy, Yantai University, Yantai 264000, PR China.
authors.forename: Xi-Yue
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Laboratory of Neuropharmacology, School of Pharmacy, Yantai University, Yantai 264000, PR China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Laboratory of Neuropharmacology, School of Pharmacy, Yantai University, Yantai 264000, PR China.
authors.forename: Meng-Lin
authors.initials: ML
authors.lastname: Zhang
authors.affiliation: Laboratory of Neuropharmacology, School of Pharmacy, Yantai University, Yantai 264000, PR China; Neurobiology Laboratory, College of Life Sciences, Peking University, Beijing 100871, PR China. Electronic address: yulc@pku.edu.cn.
authors.forename: Long-Chuan
authors.initials: LC
authors.lastname: Yu
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D019004:Galanin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D059225:Nociception; D010455:Peptides / Q000494:pharmacology*; D010487:Periaqueductal Gray / Q000187:drug effects / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D044092:Receptor, Galanin, Type 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000700:Analgesics; C000603588:M871 peptide; D010455:Peptides; D044092:Receptor, Galanin, Type 2; D019004:Galanin; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: Antinociceptive effect; Ca(2+)/calmodulin-dependent kinase II (CaMKII); Galanin; Galanin receptor 2; Hindpaw withdrawal latency; Periaqueductal grey (PAG)