title: Lentivirus‑delivered nemo‑like kinase small interfering RNA inhibits laryngeal cancer cell proliferation in vitro.
abstract: Laryngeal squamous cell carcinoma is the most common form of head and neck squamous cell carcinoma. Multiple approaches have been applied to treat this type of cancer; however, no significant improvement in survival rate has been achieved. In the present study, the role of nemo‑like kinase (NLK) in human laryngeal carcinoma Hep‑2 cells was investigated. NLK has been identiﬁed as an important regulator of cell growth, patterning and cell death in a variety of organisms. Lentivirus‑mediated‑shRNA was employed to silence endogenous NLK expression. Downregulation of the expression of NLK following lentivirus infection was confirmed using reverse transcription quantitative polymerase chain reaction and western blot analysis. The effects of NLK downregulation on Hep‑2 cell proliferation and cell cycle progression were analyzed using an MTT assay and flow cytometry, respectively. Downregulation of NLK also inhibited tumorigenesis and regulated the expression of cell cycle protein expression levels. Therefore, it was hypothesized that NLK is necessary for cell survival and tumorigenesis in laryngeal cancer cells. Furthermore, the absence of NLK may lead to cancer cell death. Collectively, the results of the present study demonstrated that the lentivirus‑mediated targeted disruption of NLK may be a promising therapeutic method for the treatment of laryngeal cancer.
authors.affiliation: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tai
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Rao
authors.affiliation: Key Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
authors.forename: Jugao
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Key Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Long
authors.affiliation: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Han
authors.affiliation: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
authors.forename: Qiaoyin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Center Laboratory, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, P.R. China.
authors.forename: Aidong
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ni
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D056744:Cyclin B1 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D019938:Cyclin D1 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D007830:Larynx / Q000378:metabolism / Q000473:pathology; D016086:Lentivirus / Q000235:genetics; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism
chemical_list: C534699:CCNB1 protein, human; C530104:CCND1 protein, human; D056744:Cyclin B1; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; C470551:NLK protein, human; D017346:Protein Serine-Threonine Kinases