title: Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes.
abstract: Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea; Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea.
authors.forename: Bieong-Kil
authors.initials: BK
authors.lastname: Kim
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Young-Bae
authors.initials: YB
authors.lastname: Seu
authors.affiliation: School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea.
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea. Electronic address: kodoh@ynu.ac.kr.
authors.forename: Kyung-Oh
authors.initials: KO
authors.lastname: Doh
mesh_terms: D000818:Animals; D019556:COS Cells; D002412:Cations / Q000737:chemistry; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000737:chemistry*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D018014:Gene Transfer Techniques; D008055:Lipids / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D008081:Liposomes / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D008968:Molecular Conformation; D015203:Reproducibility of Results
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D008055:Lipids; D008081:Liposomes; D002784:Cholesterol
keywords: Cholesterol; Fluorescent probe; Liposome; Transfection