title: IFITM1 promotes the metastasis of human colorectal cancer via CAV-1.
abstract: Interferon-induced transmembrane protein 1 (IFITM1) is one of the interferon-induced transmembrane protein family members. In this study, we reported that the elevated IFITM1 expression in human colorectal cancer (CRC) significantly correlated with CRC lymph node and distance metastasis as well as a more advanced clinical stage. Importantly, elevated IFITM1 expression is an independent prognostic factor for poor survival. To investigate the molecular mechanisms, we showed that over-expression of IFITM1 in CRC cells promoted, whereas knockdown of IFITM1 expression inhibited, cell migration/invasion and tumorigenicity in vitro. Furthermore, we identified Caveolin-1 (CAV1) as a downstream target of IFITM1-induced cell invasion, as knockdown of CAV1 abrogated siIFITM1 mediated inhibition of cell invasion in CRC cells. In addition, in a CRC cohort of 229 patients, the expression of IFITM1 inversely correlated with the expression of CAV1. These results suggested that IFITM1 promotes the aggressiveness of CRC cells, and it is a potential prognostic marker and therapeutic target for CRC.
authors.affiliation: Department of Pathology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.affiliation: Department of Chemistry, The University of Hong Kong, Hong Kong, China.
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Ng
authors.affiliation: Department of Chemistry, The University of Hong Kong, Hong Kong, China.
authors.forename: Ching Tung
authors.initials: CT
authors.lastname: Lum
authors.affiliation: Department of Pathology, Cancer Center, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Mu-Yan
authors.initials: MY
authors.lastname: Cai
authors.affiliation: Department of Chemistry, The University of Hong Kong, Hong Kong, China.
authors.forename: William K
authors.initials: WK
authors.lastname: Cheung
authors.affiliation: The HK Acad Workstation, Kunming Medical University, Kunming, China; School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.affiliation: Shenzhen Key Lab of Translational Medicine of Tumor, School of Medicine, Shenzhen University, Shenzhen, China.
authors.forename: Guimiao
authors.initials: G
authors.lastname: Lin
authors.affiliation: Shenzhen Key Lab of Translational Medicine of Tumor, School of Medicine, Shenzhen University, Shenzhen, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shenzhen Key Lab of Translational Medicine of Tumor, School of Medicine, Shenzhen University, Shenzhen, China. Electronic address: mcmlin@163.com.
authors.forename: Marie C
authors.initials: MC
authors.lastname: Lin
mesh_terms: D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D016016:Proportional Hazards Models; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D000943:Antigens, Differentiation; C495540:CAV1 protein, human; D051242:Caveolin 1; C062010:leu-13 antigen
keywords: CAV1; Colorectal cancer; IFITM1; Metastasis