title: Reduction in oxidative stress biomarkers after adenotonsillectomy.
abstract: OBJECTIVE
  A number of otolaryngic conditions such as chronic tonsillitis, adenoid hypertrophy, and obstructive sleep apnea are associated with oxidative stress and elevated levels of serum oxidants. The objective of this study is to measure changes in urine biomarkers of oxidative stress in children after adenotonsillectomy.
  
  
  METHODS
  Twenty-two children with sleep disordered breathing (SDB) with tonsil and adenoid hypertrophy were enrolled prior to adenotonsillectomy. Controls consisted of 20 healthy children. Urine samples were collected from all patients. Levels of three urinary biomarkers for oxidative status, 8-hydroxy-2-deoxyguanosine (8-OxodG), F(2)-isoprostane, and malondialdehyde (MDA) were measured using high performance liquid chromatography. For the study group, urine samples were repeated 3 weeks after surgery.
  
  
  RESULTS
  In the study group, preoperative urinary levels of 8-OxodG were higher than in controls (p=0.015). Levels decreased after surgery compared to preoperative levels (p=0.002), and reached control levels (p=0.167) at 3 weeks. Levels of urinary F(2)-isoprostane were similar in both groups (p=0.252), but decreased significantly after surgery (p=0.020).
  
  
  CONCLUSIONS
  Children with SDB have elevated levels of urinary 8-OxodG, a marker of oxidative stress. Adenotonsillectomy results in decreased 8-OxodG and F(2)-isoprostane. These findings suggest that urine analysis may represent a valuable tool for the measurement of oxidative stress.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Konkuk University, Seoul, South Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Head and Neck Surgery, UCLA School of Medicine, Los Angeles, CA, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Suh
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Konkuk University, Seoul, South Korea.
authors.forename: Yong Won
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Konkuk University, Seoul, South Korea.
authors.forename: Seok-Chan
authors.initials: SC
authors.lastname: Hong
authors.affiliation: Seegene Medical Foundation, Seoul, South Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Konkuk University, Seoul, South Korea. Electronic address: entalk@kuh.ac.kr.
authors.forename: Jin Kook
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000233:Adenoidectomy; D015415:Biomarkers / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006984:Hypertrophy / Q000601:surgery; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000652:urine*; D012891:Sleep Apnea Syndromes / Q000378:metabolism / Q000601:surgery*; D014068:Tonsillectomy; D014069:Tonsillitis / Q000378:metabolism / Q000601:surgery*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species
keywords: 8-hydroxy-2-deoxyguanosine; Adenoidectomy; F(2)-isoprostane; Malondialdehyde; Pediatric snoring; Tonsillectomy