title: Trends in the genomic epidemiology of Vibrio cholerae O1 isolated worldwide since 1961.
abstract: Here we describe the international scenario of Vibrio cholerae with a comparative analysis of different aspects of typing. Representative V. cholerae strains (n=108) associated with endemic cholera regions from 29 states of India and worldwide were subjected to microbiological, molecular and phylogenetic study. All of the strains were V. cholerae serogroup O1 biotype El Tor and were typed according to both the new phage (NP) type and Basu & Mukherjee (BM) typing schemes. The predominant phage type was T-27 (NP)/T-4 (BM) (65.7%; n=71), followed by phage type T-27 (NP)/T-2 (BM) (14.8%; n=16), T-26 (NP)/T4 (BM) (12.0%; n=13), T-13 (NP)/T-4 (BM) (2.8%; n=3), T-20 (NP)/T-4 (BM) (1.9%; n=2), T-3 (NP)/T-4 (BM) (0.9%; n=1), T-23 (NP)/T-4 (BM) (0.9%; n=1) and T-24 (NP)/T-2 (BM) (0.9%; n=1). Mismatch amplification mutation assay PCR (MAMA-PCR) findings showed the dominance of ctxB El Tor genotype (77.1%; 54/70) from 1961-1991, whilst the next two epochs showed the supremacy of ctxB classical genotype. Multidrug-resistant strains showed resistance to erythromycin, streptomycin, trimethoprim/sulfamethoxazole, norfloxacin and ampicillin. The regional resistance of epidemic clones in India draws a layout of the rapid dissemination of resistance in the past 30 years and the necessity of proper treatment to protect populations at risk.
authors.affiliation: Vibrio Phage Reference Laboratory, Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, Scheme-XM, CIT Road, Beliaghata, Kolkata 700 010, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Jaiswal
authors.affiliation: Vibrio Phage Reference Laboratory, Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, Scheme-XM, CIT Road, Beliaghata, Kolkata 700 010, India.
authors.forename: Sounak
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Vibrio Phage Reference Laboratory, Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, Scheme-XM, CIT Road, Beliaghata, Kolkata 700 010, India.
authors.forename: Parijat
authors.initials: P
authors.lastname: Das
authors.affiliation: Vibrio Phage Reference Laboratory, Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, Scheme-XM, CIT Road, Beliaghata, Kolkata 700 010, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Nandy
authors.affiliation: Vibrio Phage Reference Laboratory, Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, Scheme-XM, CIT Road, Beliaghata, Kolkata 700 010, India.
authors.forename: Hemanta
authors.initials: H
authors.lastname: Koley
authors.affiliation: Vibrio Phage Reference Laboratory, Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, Scheme-XM, CIT Road, Beliaghata, Kolkata 700 010, India. Electronic address: bl_sarkar@hotmail.com.
authors.forename: Banwarilal
authors.initials: B
authors.lastname: Sarkar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001434:Bacteriophage Typing; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D014644:Genetic Variation; D014943:Global Health; D006801:Humans; D017720:Molecular Epidemiology; D058889:Molecular Typing; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D002772:Cholera Toxin
keywords: Basu & Mukherjee type; MAMA-PCR; New phage type; PFGE; Vibrio cholerae; ctxB gene