title: Quality-by-design-based ultra high performance liquid chromatography related substances method development by establishing the proficient design space for sumatriptan and naproxen combination.
abstract: Quality-by-design-based methods hold greater level of confidence for variations and greater success in method transfer. A quality-by-design-based ultra high performance liquid chromatography method was developed for the simultaneous assay of sumatriptan and naproxen along with their related substances. The first screening was performed by fractional factorial design comprising 44 experiments for reversed-phase stationary phases, pH, and organic modifiers. The results of screening design experiments suggested phenyl hexyl column and acetonitrile were the best combination. The method was further optimized for flow rate, temperature, and gradient time by experimental design of 20 experiments and the knowledge space was generated for effect of variable on response (number of peaks ≥ 1.50 - resolution). Proficient design space was generated from knowledge space by applying Monte Carlo simulation to successfully integrate quantitative robustness metrics during optimization stage itself. The final method provided the robust performance which was verified and validated. Final conditions comprised Waters® Acquity phenyl hexyl column with gradient elution using ammonium acetate (pH 4.12, 0.02 M) buffer and acetonitrile at 0.355 mL/min flow rate and 30°C. The developed method separates all 13 analytes within a 15 min run time with fewer experiments compared to the traditional quality-by-testing approach.
authors.affiliation: Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, Telangana, India.
authors.forename: Prinesh N
authors.initials: PN
authors.lastname: Patel
authors.affiliation: University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.
authors.forename: Vijaya Saradhi
authors.initials: VS
authors.lastname: Karakam
authors.affiliation: Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, Telangana, India.
authors.forename: Gananadhamu
authors.initials: G
authors.lastname: Samanthula
authors.affiliation: University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.|National Centre for Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Ragampeta
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004338:Drug Combinations; D004340:Drug Contamination; D006801:Humans; D009010:Monte Carlo Method; D009288:Naproxen / Q000008:administration & dosage / Q000032:analysis*; D011786:Quality Control; D018170:Sumatriptan / Q000008:administration & dosage / Q000032:analysis*; D013607:Tablets / Q000032:analysis
chemical_list: D004338:Drug Combinations; D013607:Tablets; D009288:Naproxen; D018170:Sumatriptan
keywords: Design space; Impurity profiling; Naproxen; Quality by design; Sumatriptan