title: Fluconazole and Voriconazole Resistance in Candida parapsilosis Is Conferred by Gain-of-Function Mutations in MRR1 Transcription Factor Gene.
abstract: Candida parapsilosis is the second most prevalent fungal agent causing bloodstream infections. Nevertheless, there is little information about the molecular mechanisms underlying azole resistance in this species. Mutations (G1747A, A2619C, and A3191C) in the MRR1 transcription factor gene were identified in fluconazole- and voriconazole-resistant strains. Independent expression of MRR1 genes harboring these mutations showed that G1747A (G583R) and A2619C (K873N) are gain-of-function mutations responsible for azole resistance, the first described in C. parapsilosis.
authors.affiliation: Department of Microbiology, Faculty of Medicine of the University of Porto, Porto, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Branco
authors.affiliation: Department of Microbiology, Faculty of Medicine of the University of Porto, Porto, Portugal Center for Health Technology and Services Research (CINTESIS), Faculty of Medicine of the University of Porto, Porto, Portugal.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Silva
authors.affiliation: Institute for Biomedicine (iBiMED) and IEETA, University of Aveiro, Aveiro, Portugal Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal.
authors.forename: Raquel M
authors.initials: RM
authors.lastname: Silva
authors.affiliation: Department of Microbiology, Faculty of Medicine of the University of Porto, Porto, Portugal Center for Health Technology and Services Research (CINTESIS), Faculty of Medicine of the University of Porto, Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Silva-Dias
authors.affiliation: Department of Microbiology, Faculty of Medicine of the University of Porto, Porto, Portugal Center for Health Technology and Services Research (CINTESIS), Faculty of Medicine of the University of Porto, Porto, Portugal.
authors.forename: Cidália
authors.initials: C
authors.lastname: Pina-Vaz
authors.affiliation: UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Butler
authors.affiliation: Department of Microbiology, Faculty of Medicine of the University of Porto, Porto, Portugal Center for Health Technology and Services Research (CINTESIS), Faculty of Medicine of the University of Porto, Porto, Portugal.
authors.forename: Acácio G
authors.initials: AG
authors.lastname: Rodrigues
authors.affiliation: Department of Microbiology, Faculty of Medicine of the University of Porto, Porto, Portugal Center for Health Technology and Services Research (CINTESIS), Faculty of Medicine of the University of Porto, Porto, Portugal imiranda@med.up.pt.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Miranda
mesh_terms: D002175:Candida / Q000187:drug effects / Q000235:genetics; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D065819:Voriconazole / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors; D015725:Fluconazole; D065819:Voriconazole