title: Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell.
abstract: Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of suspension peripheral blood mononucleated stem cells (PBMNSCs) without resulting in loss in the differentiation potential. Mononucleated cells were isolated from both mice and human peripheral blood samples through gradient centrifugation and expanded in α-MEM, RPMI, MEM or DMEM supplemented with either NBCS or FBS. The suspension cells were then differentiated to osteoblast. Our data suggested that α-MEM supplemented with 10 % (v/v) NBCS gives the highest fold increase after 14 days of culture for both mice and human PBMNSCs, which were ~1.51 and ~2.01 times, respectively. The suspension PBMNSCs in the respective medium were also able to maintain osteoblast differentiation potential as supported by the significant increase in ALP specific activity. The cells are also viable during the differentiated states when using this media. All these data strongly suggested that α-MEM supplemented with 10 % NBCS is the best media for the expansion of both mouse and human suspension PBMNSCs.
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
authors.forename: Shahrul Hisham
authors.initials: SH
authors.lastname: Zainal Ariffin
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
authors.forename: Nur Akmal
authors.initials: NA
authors.lastname: Mohamed Rozali
authors.affiliation: Department of Orthodontic, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
authors.forename: Rohaya
authors.initials: R
authors.lastname: Megat Abdul Wahab
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia. sahidan@ukm.my.
authors.forename: Sahidan
authors.initials: S
authors.lastname: Senafi
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
authors.forename: Intan Zarina
authors.initials: IZ
authors.lastname: Zainol Abidin
authors.affiliation: Department of Microbiology, Faculty of Applied Sciences, MARA University of Technology, 40450, Shah Alam, Selangor, Malaysia.
authors.forename: Zaidah
authors.initials: Z
authors.lastname: Zainal Ariffin
keywords: Basal media; Cell differentiation; Cell expansion; Peripheral blood; Serum; Suspension stem cell