title: PLGA/alginate composite microspheres for hydrophilic protein delivery.
abstract: Poly(lactic-co-glycolic acid) (PLGA) microspheres and PLGA/alginate composite microspheres were prepared by a novel double emulsion and solvent evaporation technique and loaded with bovine serum albumin (BSA) or rabbit anti-laminin antibody protein. The addition of alginate and the use of a surfactant during microsphere preparation increased the encapsulation efficiency and reduced the initial burst release of hydrophilic BSA. Confocal laser scanning microcopy (CLSM) of BSA-loaded PLGA/alginate composite microspheres showed that PLGA, alginate, and BSA were distributed throughout the depths of microspheres; no core/shell structure was observed. Scanning electron microscopy revealed that PLGA microspheres erode and degrade more quickly than PLGA/alginate composite microspheres. When loaded with anti-laminin antibody, the function of released antibody was well preserved in both PLGA and PLGA/alginate composite microspheres. The biocompatibility of PLGA and PLGA/alginate microspheres were examined using four types of cultured cell lines, representing different tissue types. Cell survival was variably affected by the inclusion of alginate in composite microspheres, possibly due to the sensitivity of different cell types to excess calcium that may be released from the calcium cross-linked alginate.
authors.affiliation: Department of Anatomy and Cell Biology, University of Saskatchewan, S7N5E5, Canada; Division of Biomedical Engineering, University of Saskatchewan, S7N5A9, Canada.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhai
authors.affiliation: Department of Mechanical Engineering, University of Saskatchewan, S7N5A9, Canada; Division of Biomedical Engineering, University of Saskatchewan, S7N5A9, Canada.
authors.forename: X B
authors.initials: XB
authors.lastname: Chen
authors.affiliation: Department of Anatomy and Cell Biology, University of Saskatchewan, S7N5E5, Canada; Division of Biomedical Engineering, University of Saskatchewan, S7N5A9, Canada. Electronic address: david.schreyer@usask.ca.
authors.forename: David J
authors.initials: DJ
authors.lastname: Schreyer
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D016503:Drug Delivery Systems; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D007797:Laminin / Q000037:antagonists & inhibitors; D008422:Materials Testing; D051379:Mice; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000464:Alginates; D000906:Antibodies; D006603:Hexuronic Acids; D007797:Laminin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D020723:Glucuronic Acid
keywords: Alginate; Cell culture; Microspheres; PLGA; Protein release; Scanning electron microscopy