title: An EBV-based plasmid can replicate and maintain in stem cells.
abstract: Viral vectors have a wide range of applications in biology, particularly in gene therapy. Based on their integration capacity, viral vectors are classified as either integrating or non-integrating vectors. Although integrating vectors, such as lentivectors, have the ability to direct prolonged expression of exogenous genes, manipulation of the host genome is an inappropriate feature of these gene delivery tools. Non-integrating vectors, such as episomal replicating plasmids, can replicate and persist in host cells for long periods without any chromosomal interruption. These advantages made them good tools for gene induction purposes in gene therapy and basic studies. Due to the necessity of gene induction in stem cells for study of mammalian development and targeted differentiation, the use of integrating vectors for prolonged expression of genes of interest has been developed. Application of replicating plasmids can overcome some drawbacks associated with integrating vectors, although replication and maintenance of these plasmids can differ between cell types. Previously, it has been shown that such plasmids can be maintained in human embryonic stem cells for more than one month, but the rate of the plasmid replication during the host cell cycle has not been elucidated. In the present study, we showed that an EBV-based plasmid can replicate simultaneously with host in pluripotent and multipotent human and mouse stem cells and can be sustained for long time periods in dividing cells.
authors.affiliation: Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-331, Iran.|Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, 1997775555, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Ali Hosseini Rad
authors.affiliation: Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-331, Iran.
authors.forename: Taravat
authors.initials: T
authors.lastname: Bamdad
authors.affiliation: Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, 1997775555, Iran.|Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, 14155-6455, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Arefian
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Mossahebi-Mohammadi
authors.affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-175, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Sadeghizadeh
mesh_terms: D000818:Animals; D002460:Cell Line; D024882:Drug Resistance, Viral; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D013234:Stem Cells / Q000821:virology*; D014779:Virus Replication / Q000235:genetics
keywords: EBNA-1; EBV-based plasmid; oriP; stem cells