title: Effect of CoCl₂ on fracture repair in a rat model of bone fracture.
abstract: Low oxygen availability is known to activate the hypoxia-inducible factor-1α (HIF-1α) pathway, which is involved in the impairment of fracture healing. However, the role of low oxygen in fracture healing remains to be fully elucidated. In the present study, rats were divided into two groups and treated with CoCl2 or saline, respectively. Mice with tibial fractures were sacrificed at 14, 28 and 42 days subsequent to fracture. Autoradiography was performed to measure healing of the bone tissue. In addition, the effects of cobalt chloride (CoCl2) on the expression of two major angiogenic mediators, HIF‑1α and vascular endothelial growth factor (VEGF), as well as the osteoblast markers runt‑related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin (OC) were determined at mRNA and protein levels by reverse transcription‑quantitative polymerase chain reaction, western blot analysis and immunohistochemistry. Systemic administration of CoCl2 (15 mg/kg/day intraperitoneally) significantly promoted fracture healing and mechanical strength. The present study demonstrated that in rats treated with CoCl2, the expression of HIF‑1α, VEGF, Runx2, ALP and OC was significantly increased at mRNA and protein levels, and that CoCl2 treatment enhances fracture repair in vivo.
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: An
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Jiajian
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Shen
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D016723:Bone Remodeling / Q000187:drug effects; D003035:Cobalt / Q000494:pharmacology*; D004195:Disease Models, Animal; D017102:Fracture Healing / Q000187:drug effects*; D050723:Fractures, Bone / Q000378:metabolism / Q000473:pathology*; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism; D007150:Immunohistochemistry; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats
chemical_list: D051793:Hypoxia-Inducible Factor 1; D003035:Cobalt; C018021:cobaltous chloride