title: Nucleolipid bilayers: A quartz crystal microbalance and neutron reflectometry study.
abstract: POP-Ade (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyladenosine) is a biocompatible anionic nucleolipid with the DNA nucleoside, Adenosine, in the polar headgroup. We have studied the affinity of nucleic acids of different contour length, composition and structure toward supported lipid bilayers (SLB) composed of POP-Ade mixed with the zwitterionic phospholipid POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) using quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry (NR). In order to highlight the specificity of the nucleic acid interaction, the results were compared with data obtained for SLB containing the anionic phospholipid POPG (1-palmitoyl-2-oleoyl-sn-phosphatidyl-glycerol) replacing POP-Ade. Our results demonstrate that the presence of a nucleobase headgroup provides the bilayers with the ability to bind single stranded nucleic acids in a selective fashion, according to a Watson-Crick pattern. In addition the interaction with double stranded nucleic acids was strengthened. Overall, these findings represent fundamental information for the design of biocompatible DNA vectors with DNA-RNA-based amphiphiles.
authors.affiliation: Department of Chemistry and CSGI, University of Florence, Via della Lastruccia 3, 50019 Florence, Italy.
authors.forename: Costanza
authors.initials: C
authors.lastname: Montis
authors.affiliation: Institut Laue-Langevin, 71 avenue des Martyrs, BP 156, F-38000 Grenoble, France.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Gerelli
authors.affiliation: Institut Laue-Langevin, 71 avenue des Martyrs, BP 156, F-38000 Grenoble, France.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Fragneto
authors.affiliation: Physical Chemistry, Department of Chemistry, Lund University, Box 124, SE-221 00 Lund, Sweden.
authors.forename: Tommy
authors.initials: T
authors.lastname: Nylander
authors.affiliation: Department of Chemistry and CSGI, University of Florence, Via della Lastruccia 3, 50019 Florence, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Baglioni
authors.affiliation: Department of Chemistry and CSGI, University of Florence, Via della Lastruccia 3, 50019 Florence, Italy. Electronic address: debora.berti@unifi.it.
authors.forename: Debora
authors.initials: D
authors.lastname: Berti
mesh_terms: D008051:Lipid Bilayers; D009502:Neutrons; D009696:Nucleic Acids / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D008051:Lipid Bilayers; D009696:Nucleic Acids
keywords: Molecular recognition; Neutron reflectometry; Nucleolipids; Quartz crystal microbalance; Supported lipid bilayers