title: Detection of human bocavirus in nasopharyngeal aspirates versus in broncho-alveolar lavage fluids in children with lower respiratory tract infections.
abstract: To compare the presence of human bocavirus (HBoV) in nasopharyngeal aspirates (NPA) versus broncho-alveolar lavage fluids (BAL) in children with lower respiratory tract infections (LRTIs), as revealed by real-time PCR, in order to confirm the diagnostic validity of NPA samples. A retrospective 5-year study was performed from 2009 to 2014 in 1,194 patients under the age of 17 years (mean age of 3 years) that were diagnosed with LRTIs and from whom both NPA and BAL were obtained. Clinical and demographic data were recorded, and NPA and BAL samples were analyzed for HBoV-positivity by real-time PCR. Of the 1,194 patients enrolled, 65 (5.4%) patients had HBoV detected from NPA, and 61 (5.1%) had HBoV detected from BAL. For HBoV, there was a significant association between the NPA and BAL samples (P < 0.001), but the diagnostic validity was relatively low (kappa = 0.414). When real-time PCR-positivity for HBoV in BAL was used as a reference for diagnosis, NPA had a good specificity and better positive predictive validity in male patients or those younger than 3 years of age. NPA has a similar yield and a good specificity for diagnosis of LRTIs with HBoV compared to BAL. The best diagnostic validity for NPA was detected in male patients or those younger than 3 years old.
authors.affiliation: Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China.|Department of Pediatrics, Suzhou Municipal Hospital, Suzhou, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Zheng-Rong
authors.initials: ZR
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Yong-Dong
authors.initials: YD
authors.lastname: Yan
authors.affiliation: Department of Pediatrics, Suzhou Municipal Hospital, Suzhou, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: He
authors.affiliation: Department of Pediatrics, Suzhou Municipal Hospital, Suzhou, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Shao
authors.affiliation: Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yin
authors.affiliation: Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
mesh_terms: D000293:Adolescent; D012634:Bodily Secretions / Q000821:virology*; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009305:Nasopharynx / Q000821:virology*; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods*
keywords: broncho-alveolar lavage fluids; children; human bocavirus; nasopharyngeal aspirates