title: MicroRNA‑205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.
abstract: Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR‑205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR‑205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR‑205 in the proliferation, migration, invasion and apoptosis of NPC‑derived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR‑205 was identified using the luciferase reporter assay. The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR‑205. Overall, the present study demonstrated that miR‑205 may function as an oncogene in NPC tumorigenesis.
authors.affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Nie
authors.affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Ruijing
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Clinical Medicine, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University ‑ The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000328:Adult; D017209:Apoptosis; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002277:Carcinoma; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D015194:Diffusion Chambers, Culture; D004338:Drug Combinations; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000737:chemistry; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009305:Nasopharynx / Q000378:metabolism / Q000473:pathology; D009367:Neoplasm Staging; D011509:Proteoglycans / Q000737:chemistry; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; D003094:Collagen; D004338:Drug Combinations; D006360:Heat-Shock Proteins; D007797:Laminin; D008156:Luciferases; D035683:MicroRNAs; D011509:Proteoglycans; C534425:MIR205, human; C429767:TP53INP1 protein, human; C059485:matrigel