title: Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
abstract: This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials. Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs). The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA). Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures. Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
authors.affiliation: Bioelectrochemistry Lab, Department of Chemistry, University of Ankara, Tandogan, Ankara 06100, Turkey. Electronic address: burakderkus@gmail.com.
authors.forename: Burak
authors.initials: B
authors.lastname: Derkus
authors.affiliation: Bioelectrochemistry Lab, Department of Chemistry, University of Ankara, Tandogan, Ankara 06100, Turkey.
authors.forename: Kaan Cebesoy
authors.initials: KC
authors.lastname: Emregul
authors.affiliation: Bioelectrochemistry Lab, Department of Chemistry, University of Ankara, Tandogan, Ankara 06100, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Emregul
mesh_terms: D000464:Alginates / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D004337:Drug Carriers; D006603:Hexuronic Acids; D037742:Nanotubes, Carbon; D011506:Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D005780:Gelatin; D048271:Chitosan
keywords: Biomaterial; Carbon nanotube; Hydrogel; Immobilization; Nanocomposite