title: Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.
abstract: Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Jieqiong
authors.initials: J
authors.lastname: Li
authors.affiliation: The Department of Dermatology, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Shen
authors.affiliation: The Department of Dermatology, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Department of Dermatology, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Yunzhu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.affiliation: The Department of Dermatology, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shen
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qi
authors.affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.affiliation: The Department of Dermatology, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology*; D018941:Antigens, CD19 / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D018956:CD5 Antigens / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000378:metabolism*; D051379:Mice
chemical_list: D018949:Antigens, CD1; D018941:Antigens, CD19; D018956:CD5 Antigens; D007073:Immunoglobulin E