title: Transcriptome-based discovery of AP2/ERF transcription factors related to temperature stress in tea plant (Camellia sinensis).
abstract: Tea plant (Camellia sinensis) is an important natural resource for the global supply of non-alcoholic beverage production. The extension of tea plant cultivation is challenged by biotic and abiotic stresses. Transcription factors (TFs) of the APETALA 2 (AP2)/ethylene-responsive factor (ERF) family are involved in growth and anti-stresses through multifaceted transcriptional regulation in plants. This study comprehensively analyzed AP2/ERF family TFs from C. sinensis on the basis of the transcriptome sequencing data of four tea plant cultivars, namely, 'Yunnanshilixiang', 'Chawansanhao', 'Ruchengmaoyecha', and 'Anjibaicha'. A total of 89 putative AP2/ERF transcription factors with full-length AP2 domain were identified from C. sinensis and classified into five subfamilies, namely, AP2, dehydration-responsive-element-binding (DREB), ERF, related to ABI3/VP (RAV), and Soloist. All identified CsAP2/ERF genes presented relatively stable expression levels in the four tea plant cultivars. Many groups also showed cultivar specificity. Five CsAP2/ERF genes from each AP2/ERF subfamily (DREB, ERF, AP2, and RAV) were related to temperature stresses; these results indicated that AP2/ERF TFs may play important roles in abnormal temperature stress response in C. sinensis.
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wu
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Xing-Hui
authors.initials: XH
authors.lastname: Li
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Liu
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. zhuangjing@njau.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhuang
mesh_terms: D000595:Amino Acid Sequence; D028241:Camellia sinensis / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020218:Response Elements; D050656:Transcription Factor AP-2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D050656:Transcription Factor AP-2
keywords: AP2/ERF; Camellia sinensis; Temperature stresses; Transcription factors; Transcriptome sequencing