title: Sub-Nanogram Detection of RDX Explosive by Monoclonal Antibodies.
abstract: Polyclonal and monoclonal antibodies were raised to protein carrier molecules haptenized with RDX, a major component of many plastic explosives including Semtex. Sera from immunized mice detected RDX protein conjugates in standard ELISA. Clonally purified monoclonal antibodies had detection limits in the sub-ng/mL range for underivatized RDX in competition ELISA. The monoclonal antibodies are not dependent on the presence of taggants added during the manufacturing process, and are likely to have utility in the detection of any explosive containing RDX, or RDX contamination of environmental sites.
authors.affiliation: 1 Biomedical Sciences Department, Dstl Porton Down , Salisbury, United Kingdom .
authors.forename: David O
authors.initials: DO
authors.lastname: Ulaeto
authors.affiliation: 2 Detection Department, Dstl Porton Down , Salisbury, United Kingdom .
authors.forename: Alistair P
authors.initials: AP
authors.lastname: Hutchinson
authors.affiliation: 2 Detection Department, Dstl Porton Down , Salisbury, United Kingdom .
authors.forename: Stephen
authors.initials: S
authors.lastname: Nicklin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D014227:Triazines / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D014227:Triazines; C009160:cyclonite