title: Bufei Yishen granule combined with acupoint sticking improves pulmonary function and morphormetry in chronic obstructive pulmonary disease rats.
abstract: BACKGROUND
  The integrated therapy of Bufei Yishen granule and acupoint sticking has been used in the treatment of stable chronic obstructive pulmonary disease (COPD) clinically, with remarkable benefits. This study was initiated to observe the effects of the combination of Bufei Yishen granule and acupoint sticking on pulmonary function and morphormetry in a COPD rat model.
  
  
  METHODS
  Rats were randomized into Control, Model, Bufei Yishen (BY), Acupoint sticking (AS), Bufei Yishen + Acupoint sticking (BY + AS) and aminophyline (APL) groups. COPD rats were duplicated by repeated cigarette smoke and bacterial exposures. The rats were treated with normal saline, Bufei Yishen granule, acupoint sticking, Bufei Yishen + Acupoint sticking and aminophylline, respectively, from week 9 through 20. Pulmonary function was measured by using a whole body plethysmograph every 4 weeks. The rats were sacrificed at the end of week 20, and lung tissue histology and ultrastructure was observed under light and electron microscopes.
  
  
  RESULTS
  The pulmonary function, including tidal volume (VT), peak expiratory flow (PEF) and expiratory flow at 50 % tidal volume (EF50), was markedly decreased from week 8 in COPD rats (P < 0.05). At week 20, VT, PEF and EF50 were significantly lower in Model group (P < 0.05). Compared with Model group, VT, PEF and EF50 were higher in BY and BY + AS groups (P < 0.05), and EF50 was higher in AS group, while VT was higher in APL group (P < 0.05). Markedly histological and ultrastructural changes, including respiratory membrane thickening, volume density of lamellar corpuscle decreasing, mitochondria reducing in type II alveolar cell, were found in COPD rats and were alleviated in the treated groups, especially in BY and BY + AS groups.
  
  
  CONCLUSIONS
  Bufei Yishen granule and acupoint sticking can improve pulmonary function and lung pathological impairment in COPD rats, the curative effect of the combination is better than acupoint sticking or aminophylline only.
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. yange0910@126.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. yange0910@126.com.
authors.forename: Yange
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. yuanfengtcm@126.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. yuanfengtcm@126.com.|Central Laboratory, the First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, 450008, Henan Province, China. yuanfengtcm@126.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. li_js8@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. li_js8@163.com.|Institute for Respiratory Diseases, the First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Longzihu University Town, Zhengdong New District, Zhengzhou, 450008, Henan Province, China. li_js8@163.com.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. xieyanghn@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. xieyanghn@163.com.|Institute for Respiratory Diseases, the First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Longzihu University Town, Zhengdong New District, Zhengzhou, 450008, Henan Province, China. xieyanghn@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. wmh107hn@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. wmh107hn@163.com.|Institute for Respiratory Diseases, the First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Longzihu University Town, Zhengdong New District, Zhengzhou, 450008, Henan Province, China. wmh107hn@163.com.
authors.forename: Minghang
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. dyq865239@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. dyq865239@163.com.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. liheli90@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. liheli90@163.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. 13838149747@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. 13838149747@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. lucy6835@163.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. lucy6835@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute for Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, Henan Province, China. hnzyglyst@126.com.|The collaborative innovation center for Respiratory Diseases Diagnostics, Treatment and New Drug Research and Development, Zhengzhou, 450046, Henan Province, China. hnzyglyst@126.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Luo
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D051381:Rats
chemical_list: C574914:Bu-Fei-Yi-Shen; D004365:Drugs, Chinese Herbal