title: Differential mRNA Accumulation upon Early Arabidopsis thaliana Infection with ORMV and TMV-Cg Is Associated with Distinct Endogenous Small RNAs Level.
abstract: Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions. Several studies have highlighted the relationship between viral infections, endogenous sRNA accumulation and transcriptional changes associated with symptoms. However, few studies have described a global analysis of endogenous sRNAs by comparing related viruses at early stages of infection, especially before viral accumulation reaches systemic tissues. An sRNA high-throughput sequencing of Arabidopsis thaliana leaf samples infected either with Oilseed rape mosaic virus (ORMV) or crucifer-infecting Tobacco mosaic virus (TMV-Cg) with slightly different symptomatology at two early stages of infection (2 and 4 dpi) was performed. At early stages, both viral infections strongly alter the patterns of several types of endogenous sRNA species in distal tissues with no virus accumulation suggesting a systemic signaling process foregoing to virus spread. A correlation between sRNAs derived from protein coding genes and the associated mRNA transcripts was also detected, indicating that an unknown recursive mechanism is involved in a regulatory circuit encompassing this sRNA/mRNA equilibrium. This work represents the initial step in uncovering how differential accumulation of endogenous sRNAs contributes to explain the massive alteration of the transcriptome associated with plant-virus interactions.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Zavallo
authors.affiliation: Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), INTA, Córdoba, Argentina.
authors.forename: Humberto Julio
authors.initials: HJ
authors.lastname: Debat
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Conti
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina.
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Manacorda
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Rodriguez
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Asurmendi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000821:virology*; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D009029:Mosaic Viruses; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger