title: Simultaneous quantitation of acetylsalicylic acid and clopidogrel along with their metabolites in human plasma using liquid chromatography tandem mass spectrometry.
abstract: The interest in therapeutic drug monitoring has increased over the last few years. Inter- and intra-patient variability in pharmacokinetics, plasma concentration related toxicity and success of therapy have stressed the need of frequent therapeutic drug monitoring of the drugs. A sensitive, selective and rapid liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous quantification of acetylsalicylic acid (aspirin), salicylic acid, clopidogrel and carboxylic acid metabolite of clopidogrel in human plasma. The chromatographic separations were achieved on Waters Symmetry Shield(TM) C18 column (150 × 4.6 mm, 5 µm) using 3.5 mm ammonium acetate (pH 3.5)-acetonitrile (10:90, v/v) as mobile phase at a flow rate of 0.75 mL/min. The present method was successfully applied for therapeutic drug monitoring of aspirin and clopidogrel in 67 patients with coronary artery disease.
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.|Academy of Scientific and Innovative Research, Anusandhan Bhawan, Rafi Marg, New Delhi, 110 001, India.
authors.forename: Yashpal S
authors.initials: YS
authors.lastname: Chhonker
authors.affiliation: Pharmacology Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.|Department of Cardiology, King George's Medical University, Lucknow, 226001, India.
authors.forename: Chandra P
authors.initials: CP
authors.lastname: Pandey
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.|Academy of Scientific and Innovative Research, Anusandhan Bhawan, Rafi Marg, New Delhi, 110 001, India.
authors.forename: Hardik
authors.initials: H
authors.lastname: Chandasana
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.|Academy of Scientific and Innovative Research, Anusandhan Bhawan, Rafi Marg, New Delhi, 110 001, India.
authors.forename: Tulsankar Sachin
authors.initials: TS
authors.lastname: Laxman
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.|Academy of Scientific and Innovative Research, Anusandhan Bhawan, Rafi Marg, New Delhi, 110 001, India.
authors.forename: Yarra Durga
authors.initials: YD
authors.lastname: Prasad
authors.affiliation: Department of Cardiology, King George's Medical University, Lucknow, 226001, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Narain
authors.affiliation: Pharmacology Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.
authors.forename: Madhu
authors.initials: M
authors.lastname: Dikshit
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR - Central Drug Research Institute, Lucknow, 226031, India.|Academy of Scientific and Innovative Research, Anusandhan Bhawan, Rafi Marg, New Delhi, 110 001, India.
authors.forename: Rabi S
authors.initials: RS
authors.lastname: Bhatta
mesh_terms: D001241:Aspirin / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D000077144:Clopidogrel; D016903:Drug Monitoring; D004355:Drug Stability; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013988:Ticlopidine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism
chemical_list: D000077144:Clopidogrel; D013988:Ticlopidine; D001241:Aspirin
keywords: LC-MS/MS; acetylsalicylic acid; clopidogrel; pharmacokinetics; therapeutic drug monitoring