title: Single molecular recognition force spectroscopy study of a DNA aptamer with the target epithelial cell adhesion molecule.
abstract: The epithelial cell adhesion molecule (EpCAM) is a tumor-specific antigen for malignancies of the epithelialis lineage. In this study the interaction between the DNA-based EpCAM aptamer (SYL3C) and EpCAM was explored using single molecular recognition force spectroscopy (SMFS). The capability of aptamer SYL3C to recognize the EpCAM protein and the kinetic parameters were investigated.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. jltang@ciac.ac.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Huiqing
authors.initials: H
authors.lastname: Liu
authors.forename: Jinhui
authors.initials: J
authors.lastname: Hao
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Bai
authors.forename: Huiyan
authors.initials: H
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D052157:Aptamers, Nucleotide; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule