title: Involvement of quorum sensing genes in biofilm development and degradation of polycyclic aromatic hydrocarbons by a marine bacterium Pseudomonas aeruginosa N6P6.
abstract: Biofilm-forming and acyl homoserine lactone (AHL) synthase-positive Pseudomonas aeruginosa N6P6 was isolated from seawater after selective enrichment with two polycyclic aromatic hydrocarbons (PAHs), viz. phenanthrene and pyrene. AHL synthesis was detected qualitatively using bioreporter strains. This marine bacterium putatively synthesized N-(3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone, which were identified by TLC, GC-MS, and HPLC. Two quorum sensing (QS) genes coding for AHL synthase, i.e., lasI and rhlI, were identified in the bacterium. lasI and rhlI gene expression was studied during biofilm mode of growth at different phases using quantitative real-time PCR (qRT-PCR). The expression of lasI increased with increase in biofilm growth. In contrast, the expression of rhlI decreased during log phase of biofilm growth. The changes in lasI/rhlI expression level had significant effects (P<0.05) on biofilm architecture and subsequent PAH degradation rate. Degradation of phenanthrene and pyrene by P. aeruginosa N6P6 was affected by biofilm growth and lasI expression. The respective phenanthrene degradation for 15, 24, 48, and 72 h old biofilm after 7 days was 21.5, 54.2, 85.6, and 85.7%. However, the corresponding pyrene degradation was 15, 18.28, 47.56, and 46.48%, respectively, after 7 days. A significant positive correlation (P<0.05) was observed between lasI expression and PAHs degradation. However, in the presence of tannic acid, a QS inhibitor (QSI), PAHs degradation, biofilm formation, and pyocyanin production reduced significantly which confirmed the pivotal role of QS in biodegradation of PAHs. The findings suggest that AHLs play a pivotal role during biofilm development and subsequent bioremediation of PAHs.
authors.affiliation: Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India.
authors.forename: Neelam
authors.initials: N
authors.lastname: Mangwani
authors.affiliation: Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India.
authors.forename: Supriya
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India. surajit@nitrkl.ac.in.
authors.forename: Surajit
authors.initials: S
authors.lastname: Das
mesh_terms: D054742:Acyl-Butyrolactones / Q000032:analysis; D018441:Biofilms / Q000254:growth & development*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D053038:Quorum Sensing; D060888:Real-Time Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology*
chemical_list: D054742:Acyl-Butyrolactones; D011084:Polycyclic Aromatic Hydrocarbons
keywords: Acyl homoserine lactones; Biodegradation; Biofilm; Gene expression; PAHs; Tannic acid; lasI; rhlI