title: Highly sensitive and selective uric acid biosensor based on a three-dimensional graphene foam/indium tin oxide glass electrode.
abstract: A three-dimensional (3D) continuous and interconnected network graphene foam (GF) was synthesized by chemical vapor deposition using nickel foam as a template. The morphologies of the GF were observed by scanning electron microscopy. X-ray diffraction and Raman spectroscopy were used to investigate the structure of GF. The graphene with few layers and defect free was closely coated on the backbone of the 3D nickel foam. After etching nickel, the GF was transferred onto indium tin oxide (ITO) glass, which acted as an electrode to detect uric acid using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The GF/ITO electrode showed a high sensitivity for the detection of uric acid: approximately 9.44 mA mM(-1) in the range of 25 nM-0.1 μM and 1.85 mA mM(-1) in the range of 0.1-60 μM. The limit of detection of GF/ITO electrode for uric acid is 3 nM. The GF/ITO electrode also showed a high selectivity for the detection of uric acid in the presence of ascorbic acid. This electrode will have a wide range of potential application prospects in electrochemical detection.
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China. Electronic address: hyyue@hrbust.edu.cn.
authors.forename: Hong Yan
authors.initials: HY
authors.lastname: Yue
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chang
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, PR China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China.
authors.forename: Long Hui
authors.initials: LH
authors.lastname: Yao
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China.
authors.forename: Xuan Yu
authors.initials: XY
authors.lastname: Lin
authors.affiliation: School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150040, PR China.
authors.forename: Er Jun
authors.initials: EJ
authors.lastname: Guo
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D002681:China; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional; D007204:Indium / Q000737:chemistry; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D013059:Spectrum Analysis, Raman; D017971:Tin Compounds / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D017971:Tin Compounds; D007204:Indium; D014527:Uric Acid; D006108:Graphite; C045358:stannic oxide; D001205:Ascorbic Acid
keywords: Ascorbic acid; Biosensor; Chemical vapor deposition; Graphene foam; Nickel foam; Uric acid