title: ITS2 Database V: Twice as Much.
abstract: The internal transcribed spacer 2 (ITS2) is a well-established marker for phylogenetic analyses in eukaryotes. A reliable resource for reference sequences and their secondary structures is the ITS2 database (http://its2.bioapps.biozentrum.uni-wuerzburg.de/). However, the database was last updated in 2011. Here, we present a major update of the underlying data almost doubling the number of entities. This increases the number of taxa represented within all major eukaryotic clades. Moreover, additional data has been added to underrepresented groups and some new groups have been added. The broader coverage across the tree of life improves phylogenetic analyses and the capability of ITS2 as a DNA barcode.
authors.affiliation: Department of Animal Ecology and Tropical Biology, Julius Maximilian University, Würzburg, Germany.
authors.forename: Markus J
authors.initials: MJ
authors.lastname: Ankenbrand
authors.affiliation: Department of Animal Ecology and Tropical Biology, Julius Maximilian University, Würzburg, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Keller
authors.affiliation: Department of Bioinformatics, Julius Maximilian University, Würzburg, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wolf
authors.affiliation: Department of Bioinformatics, Julius Maximilian University, Würzburg, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schultz
authors.affiliation: Department of Bioinformatics, Julius Maximilian University, Würzburg, Germany frank.foerster@biozentrum.uni-wuerzburg.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Förster
mesh_terms: D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D030561:Databases, Nucleic Acid; D056890:Eukaryota / Q000235:genetics; D020407:Internet; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D016415:Sequence Alignment / Q000379:methods
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: barcoding; database; internal transcribed spacer; phylogeny; sequence-structure; toolbox