title: Genomic and transcriptomic insights into the cytochrome P450 monooxygenase gene repertoire in the rice pest brown planthopper, Nilaparvata lugens.
abstract: The cytochrome P450 monooxygenase (P450) gene family is one of the most abundant eukaryotic gene families that encode detoxification enzymes. In this study, we identified an abundance of P450 gene repertoire through genome- and transcriptome-wide analysis in the brown planthopper (Nilaparvata lugens), the most destructive rice pest in Asia. Detailed gene information including the exon-intron organization, size, transcription orientation and distribution in the genome revealed that many P450 loci were closely situated on the same scaffold, indicating frequent occurrence of gene duplications. Insecticide-response expression profiling revealed that imidacloprid significantly increased NlCYP6CS1v2, NLCYP4CE1v2, NlCYP4DE1, NlCYP417A1v2 and NlCYP439A1 expression; while triazophos and deltamethrin notably enhanced NlCYP303A1 expression. Expression analysis at the developmental stage showed the egg-, nymph-, male- and female-specific expression patterns of N. lugens P450 genes. These novel findings will be helpful for clarifying the P450 functions in physiological processes including development, reproduction and insecticide resistance in this insect species.
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Lao
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hai-Jian
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: yybao@zju.edu.cn.
authors.forename: Yan-Yuan
authors.initials: YY
authors.lastname: Bao
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017344:Genes, Insect; D023281:Genomics; D006430:Hemiptera / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D007093:Imidazoles / Q000494:pharmacology; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D000073943:Neonicotinoids; D009570:Nitriles / Q000494:pharmacology; D009574:Nitro Compounds / Q000494:pharmacology; D009758:Nymph / Q000201:enzymology / Q000235:genetics; D063086:Organothiophosphates / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009570:Nitriles; D009574:Nitro Compounds; D063086:Organothiophosphates; D011722:Pyrethrins; D014230:Triazoles; C017180:decamethrin; C082359:imidacloprid; C012584:triazophos; D003577:Cytochrome P-450 Enzyme System
keywords: Cytochrome P450 monooxygenase; Insecticides; Planthopper