title: Kineococcus gypseus sp. nov., isolated from saline sediment.
abstract: A novel Gram-stain-positive, aerobic, motile, non-spore-forming coccus-shaped actinomycete, designated strain YIM 121300T, was isolated from alkaline sediment in Yuanjiang, Yunnan province, south-west China. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain YIM 121300T was affiliated to the genus Kineococcus, and was closely related to Kineococcus aurantiacus IFO 15268T (97.3 % similarity). 16S rRNA gene sequence similarity to other species of the genus Kineococcus was < 97 %. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugars contained arabinose, galactose, glucose, mannose and ribose. The predominant menaquinone was MK-9(H2). Mycolic acids were not detected. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The predominant fatty acids were anteiso-C15 : 0 and anteiso-C15 : 1 A. The G+C content of the genomic DNA was 75.1 mol%. DNA–DNA relatedness (55 ± 4 % to K. aurantiacus IFO 15268T) and differential phenotypic data demonstrated that strain YIM 121300T was distinguished from all related species of the genus Kineococcus. On the basis of data from the present polyphasic study, the organism should be assigned to a novel species of the genus Kineococcus, for which the name Kineococcus gypseus sp. nov. is proposed. The type strain is YIM 121300T ( = CCTCC AA 2013232T = DSM 27627T).
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.|Life Sciences Lab Center, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: Qinyuan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: Guiding
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Microbial pharmaceuticals, College of Life and Health Science, Northeastern University, 110819 Shenyang, PR China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: Fangji
authors.initials: F
authors.lastname: Xu
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: LiHua
authors.initials: L
authors.lastname: Xu
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid