title: Identification of an RNA element for specific coordination of A-to-I RNA editing on HTR2C pre-mRNA.
abstract: Adenosine-to-Inosine (A-to-I) RNA editing is an intracellular mechanism in which inosine is specifically substituted against adenosine by the action of adenosine deaminases acting on RNA (ADARs). Serotonin 2C receptor (HTR2C) is encoded through combinatorial A-to-I RNA editing at recoding sites (A - E site) on its pre-mRNA. Although the efficiency of RNA editing at particular sites is known to be critical for modulating the serotonin signaling, the mechanistic details of site-specific editing on HTR2C pre-mRNA are not fully understood. Toward complete understanding of this mechanism, we discovered an RNA element, which coordinates site-specific RNA editing on HTR2C pre-mRNA by an in vitro editing assay and secondary structural analysis of mutant HTR2C RNA fragments. Our results showed that HTR2C pre-mRNA forms a characteristic structure, which was restricted by the internal loop and Watson-Crick base-pair interaction on site E, for intrinsic editing. We suggest that the internal loop would contribute toward adjusting the relative distance and/or geometry between the editing sites and the scaffold for ADAR.
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma 8-19-1, Jonan-ku, Fukuoka, 814-0180, Japan.
authors.forename: Masatora
authors.initials: M
authors.lastname: Fukuda
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma 8-19-1, Jonan-ku, Fukuoka, 814-0180, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Oyama
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma 8-19-1, Jonan-ku, Fukuoka, 814-0180, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Nishitarumizu
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma 8-19-1, Jonan-ku, Fukuoka, 814-0180, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Omura
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma 8-19-1, Jonan-ku, Fukuoka, 814-0180, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Nose
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma 8-19-1, Jonan-ku, Fukuoka, 814-0180, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Deshimaru
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D006801:Humans; D007288:Inosine / Q000378:metabolism; D009154:Mutation; D009690:Nucleic Acid Conformation; D017393:RNA Editing; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics* / Q000378:metabolism*; D038621:Regulatory Sequences, Ribonucleic Acid
chemical_list: D012322:RNA Precursors; D044405:Receptor, Serotonin, 5-HT2C; D038621:Regulatory Sequences, Ribonucleic Acid; D007288:Inosine; D000243:Adenosine Deaminase; D000241:Adenosine