title: Online probing quantum dots and engineered enzyme self-assembly in a nanoliter scale.
abstract: Nanoparticles provide significantly enhanced binding characteristics. However, fast online probing of the self-assembly process remains hard to achieve in practice. Herein, we report a fluorescence coupled CE method for probing the self-assembly events between quantum dots (QDs) and engineered Jumonji domain-containing protein 6 (Jmjd6) enzyme. QDs and Jmjd6 were sequentially injected into the capillary, where the self-assembly took place in a nanoliter scale. In particular, we showed that the Jmjd6/QD ratio, the interval time, and the injection volume had a great effect on the online self-assembly. The current approach may allow for a better understanding of QDs-enzyme self-assembly and enzymatic activity detection.
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jinchen
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Cheli
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Tiwan
authors.initials: T
authors.lastname: Teng
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Dong
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.|State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu, P. R. China.
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045663:Quantum Dots / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; C498707:JMJD6 protein, human; D056484:Jumonji Domain-Containing Histone Demethylases
keywords: Capillary electrophoresis; Jumonji domain-containing protein 6; Quantum dots; Self-assembly