title: A rapid UPLC-MS/MS method for the determination of oleanolic acid in rat plasma and liver tissue: application to plasma and liver pharmacokinetics.
abstract: A reliable high-throughput ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for oleanolic acid (OA) determination in rat plasma and liver tissue using glycyrrhetic acid as the internal standard (IS). Plasma and liver homogenate samples were prepared using solid-phase extraction. Chromatographic separation was achieved on a C18 column using an isocratic mobile phase system. The detection was performed by multiple reaction monitoring mode via positive electrospray ionization interface. The calibration curves showed good linearity (R(2) > 0.9997) within the tested concentration ranges. The lower limit of quantification for plasma and liver tissue was ≤0.75 ng/mL. The intra- and inter-day precision and accuracy deviations were within ±15% in plasma and liver tissue. The mean extraction recoveries ranged from 80.8 to 87.0%. In addition, the carryover, matrix effect, stability and robustness involved in the method were also validated. The method was successfully applied to the plasma and hepatic pharmacokinetics of OA after oral administration to rats.
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, People's Republic of China.|The Center for Drug Research and Development, Lianyungang College of Traditional Chinese Medicine of Jiangsu Province, Lianyungang, 222007, People's Republic of China.
authors.forename: Tian-xue
authors.initials: TX
authors.lastname: Li
authors.affiliation: The Center for Drug Research and Development, Lianyungang College of Traditional Chinese Medicine of Jiangsu Province, Lianyungang, 222007, People's Republic of China.
authors.forename: Chao-sen
authors.initials: CS
authors.lastname: Chu
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, People's Republic of China.
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Nanjing, 211198, People's Republic of China.
authors.forename: Tian-yi
authors.initials: TY
authors.lastname: Yang
authors.affiliation: Analysis and Test Center, Nanjing Normal University, Nanjing, 210023, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Center for Drug Research and Development, Lianyungang College of Traditional Chinese Medicine of Jiangsu Province, Lianyungang, 222007, People's Republic of China.
authors.forename: Yu-tao
authors.initials: YT
authors.lastname: Hu
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, People's Republic of China.
authors.forename: Xing-hao
authors.initials: XH
authors.lastname: Yang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D008099:Liver / Q000737:chemistry*; D008297:Male; D009828:Oleanolic Acid / Q000032:analysis* / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009828:Oleanolic Acid
keywords: UPLC-MS/MS; bio-analysis; liver tissue; oleanolic acid; solid-phase extraction