title: Analysis of the differences in whole-genome expression related to asthma and obesity.
abstract: BACKGROUND
  Concomitant obesity significantly impairs asthma control. Obese asthmatics show more severe symptoms and an increased use of medications.
  
  
  OBJECTIVE
  The primary aim of the study was to identify genes that are differentially expressed in the peripheral blood of asthmatic patients with obesity, asthmatic patients with normal body mass, and obese patients without asthma. Secondly, we investigated whether the analysis of gene expression in peripheral blood may be helpful in the differential diagnosis of obese patients who present with symptoms similar to asthma.
  
  
  METHODS
  The study group included 15 patients with asthma (9 obese and 6 normal-weight patients), while the control group-13 obese patients in whom asthma was excluded. The analysis of whole-genome expression was performed on RNA samples isolated from peripheral blood.
  
  
  RESULTS
  The comparison of gene expression profiles between asthmatic patients with obesity and those with normal body mass revealed a significant difference in 6 genes. The comparison of the expression between controls and normal-weight patients with asthma showed a significant difference in 23 genes. The analysis of genes with a different expression revealed a group of transcripts that may be related to an increased body mass (PI3, LOC100008589, RPS6KA3, LOC441763, IFIT1, and LOC100133565). Based on gene expression results, a prediction model was constructed, which allowed to correctly classify 92% of obese controls and 89% of obese asthmatic patients, resulting in the overall accuracy of the model of 90.9%.
  
  
  CONCLUSIONS
  The results of our study showed significant differences in gene expression between obese asthmatic patients compared with asthmatic patients with normal body mass as well as in obese patients without asthma compared with asthmatic patients with normal body mass.
authors.forename: Marta
authors.initials: M
authors.lastname: Gruchała-Niedoszytko
authors.forename: Marek
authors.initials: M
authors.lastname: Niedoszytko
authors.forename: Bahram
authors.initials: B
authors.lastname: Sanjabi
authors.forename: Pieter
authors.initials: P
authors.lastname: van der Vlies
authors.forename: Piotr
authors.initials: P
authors.lastname: Niedoszytko
authors.forename: Ewa
authors.initials: E
authors.lastname: Jassem
authors.forename: Sylwia
authors.initials: S
authors.lastname: Małgorzewicz
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000150:complications* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications* / Q000235:genetics; D012313:RNA / Q000097:blood; D059467:Transcriptome; D055815:Young Adult
chemical_list: D012313:RNA