title: Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
abstract: Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.
authors.affiliation: Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Liliana
authors.initials: L
authors.lastname: Wroblewska
authors.affiliation: Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Tasuku
authors.identifier: 0000000322653363
authors.initials: T
authors.lastname: Kitada
authors.affiliation: Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Endo
authors.affiliation: Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Velia
authors.initials: V
authors.lastname: Siciliano
authors.affiliation: Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Breanna
authors.initials: B
authors.lastname: Stillo
authors.affiliation: 1] Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. [2] The Hakubi Center for Advanced Research, Kyoto University, Kyoto, Japan.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Saito
authors.affiliation: Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Ron
authors.initials: R
authors.lastname: Weiss
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D053263:Gene Regulatory Networks / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA