title: A charged aerosol detector/chemiluminescent nitrogen detector/liquid chromatography/mass spectrometry system for regular and fragment compound analysis in drug discovery.
abstract: In this paper, we introduce a high throughput LCMS/UV/CAD/CLND system that improves upon previously reported systems by increasing both the quantitation accuracy and the range of compounds amenable to testing, in particular, low molecular weight "fragment" compounds. This system consists of a charged aerosol detector (CAD) and chemiluminescent nitrogen detector (CLND) added to a LCMS/UV system. Our results show that the addition of CAD and CLND to LCMS/UV is more reliable for concentration determination for a wider range of compounds than either detector alone. Our setup also allows for the parallel analysis of each sample by all four detectors and so does not significantly increase run time per sample.
authors.affiliation: Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. Electronic address: yutaoj@gene.com.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hascall
authors.affiliation: Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Delia
authors.initials: D
authors.lastname: Li
authors.affiliation: Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Pease
mesh_terms: D000336:Aerosols / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D055808:Drug Discovery / Q000379:methods*; D049449:Luminescence; D013058:Mass Spectrometry / Q000379:methods; D008970:Molecular Weight; D009584:Nitrogen / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*
chemical_list: D000336:Aerosols; D004364:Pharmaceutical Preparations; D009584:Nitrogen
keywords: Charged aerosol detector; Chemiluminescent nitrogen detector; Fragment; Liquid chromatography; Pharmaceutical; Purity