title: Active-Site Engineering of ω-Transaminase for Production of Unnatural Amino Acids Carrying a Side Chain Bulkier than an Ethyl Substituent.
abstract: ω-Transaminase (ω-TA) is a promising enzyme for use in the production of unnatural amino acids from keto acids using cheap amino donors such as isopropylamine. The small substrate-binding pocket of most ω-TAs permits entry of substituents no larger than an ethyl group, which presents a significant challenge to the preparation of structurally diverse unnatural amino acids. Here we report on the engineering of an (S)-selective ω-TA from Ochrobactrum anthropi (OATA) to reduce the steric constraint and thereby allow the small pocket to readily accept bulky substituents. On the basis of a docking model in which L-alanine was used as a ligand, nine active-site residues were selected for alanine scanning mutagenesis. Among the resulting variants, an L57A variant showed dramatic activity improvements in activity for α-keto acids and α-amino acids carrying substituents whose bulk is up to that of an n-butyl substituent (e.g., 48- and 56-fold increases in activity for 2-oxopentanoic acid and L-norvaline, respectively). An L57G mutation also relieved the steric constraint but did so much less than the L57A mutation did. In contrast, an L57V substitution failed to induce the improvements in activity for bulky substrates. Molecular modeling suggested that the alanine substitution of L57, located in a large pocket, induces an altered binding orientation of an α-carboxyl group and thereby provides more room to the small pocket. The synthetic utility of the L57A variant was demonstrated by carrying out the production of optically pure L- and D-norvaline (i.e., enantiomeric excess [ee]>99%) by asymmetric amination of 2-oxopantanoic acid and kinetic resolution of racemic norvaline, respectively.
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Han
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: Eul-Soo
authors.initials: ES
authors.lastname: Park
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Dong
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, South Korea enzymo@yonsei.ac.kr.
authors.forename: Jong-Shik
authors.initials: JS
authors.lastname: Shin
mesh_terms: D019943:Amino Acid Substitution; D000596:Amino Acids / Q000096:biosynthesis*; D020134:Catalytic Domain; D062105:Molecular Docking Simulation; D016297:Mutagenesis, Site-Directed; D020621:Ochrobactrum anthropi / Q000201:enzymology*; D015202:Protein Engineering; D000637:Transaminases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D000637:Transaminases