title: Biological evaluation of two Keggin-type polyoxometalates containing glycine as mushroom tyrosinase inhibitors.
abstract: Two Keggin-type polyoxometalates (POMs) containing glycine, (HGly)3PW12O40and (HGly)4SiW12O40, were synthesized and evaluated as mushroom tyrosinase inhibitors. The spectrophotometric method results showed that both (HGly)3PW12O40and (HGly)4SiW12O40could strongly inhibit the diphenolase activity of the tyrosinase and that their inhibition mechanisms were reversible. Their half-inhibition concentration values were estimated to be 1.55 and 1.39 mmol/L, respectively. The inhibition kinetics analysis by Lineweaver-Burk plots indicated that (HGly)3PW12O40was an uncompetitive inhibitor with KIS= 0.046 mmol/L, whereas (HGly)4SiW12O40was a noncompetitive inhibitor with KI= KIS= 2.17 mmol/L. This study may help to extend the application of POMs in the fields of medicine and food preservation.
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen, People's Republic of China.
authors.forename: Aping
authors.initials: A
authors.lastname: Zheng
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen, People's Republic of China. wanglimerry@jmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Fei
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou, People's Republic of China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D005998:Glycine / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017973:Tungsten Compounds / Q000737:chemistry* / Q000494:pharmacology*; D000099294:Polyoxometalates
chemical_list: D004791:Enzyme Inhibitors; D005998:Glycine; D014442:Monophenol Monooxygenase; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
keywords: diphenolase activity; food preservation; kinetic analysis; medicine; polyoxometalates; tyrosinase