title: [Development of Efficient Separation and Sensitive Analytical Methods for Biological Compounds and Their Application].
abstract: Measurement of biological compounds is important for the clarification of biological phenomena. For the quantification of trace amounts of biological compounds, efficient separation and sensitive analytical methods are necessary. The present author developed HPLC-fluorescence and chemiluminescence detection methods for biological compounds such as catecholamines, amino acids, and thiols. In this review article, two studies are summarized: one on the development of an on-chip liquid chromatography method using pillar array columns with low-dispersion turns; and another on the development of simultaneous analytical method of biothiols by HPLC with fluorescence detection under hydrophilic interaction chromatography conditions.
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Tokyo.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunoda
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D002395:Catecholamines / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D005453:Fluorescence; D057927:Hydrophobic and Hydrophilic Interactions; D008163:Luminescent Measurements / Q000379:methods; D046228:Microarray Analysis; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D002395:Catecholamines; D013438:Sulfhydryl Compounds