title: MC3T3-E1 cell response to stainless steel 316L with different surface treatments.
abstract: In the present study, stainless steel 316L samples with polishing, aluminum oxide blasting, and hydroxyapatite (HA) coating were prepared and characterized through a scanning electron microscope (SEM), optical interferometer (surface roughness, Sq), contact angle, surface composition and phase composition analyses. Osteoblast-like MC3T3-E1 cell adhesion on the samples was investigated by cell morphology using a SEM (4h, 1d, 3d, 7d), and cell proliferation was assessed by MTT method at 1d, 3d, and 7d. In addition, adsorption of bovine serum albumin on the samples was evaluated at 1h. The polished sample was smooth (Sq: 1.8nm), and the blasted and HA coated samples were much rougher (Sq: 3.2μm and 7.8μm). Within 1d of incubation, the HA coated samples showed the best cell morphology (e.g., flattened shape and complete spread), but there was no significant difference after 3d and 7d of incubation for all the samples. The absorbance value for the HA coated samples was the highest after 1d and 3d of incubation, indicating better cell viability. However, it reduced to the lowest value at 7d. Protein adsorption on the HA coated samples was the highest at 1h. The results indicate that rough stainless steel surface improves cell adhesion and morphology, and HA coating contributes to superior cell adhesion, but inhibits cell proliferation.
authors.affiliation: State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Dental Materials Laboratory, National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081, China. Electronic address: siyanghan@163.com.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Han
authors.affiliation: State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Jinghang Biomedicine Engineering Division, Beijing Institute of Aeronautical Material, Beijing 100095, China.
authors.forename: Yongling
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000537:Aluminum Oxide / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D013193:Stainless Steel / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects*
chemical_list: D020099:Coated Materials, Biocompatible; D013193:Stainless Steel; D017886:Durapatite; D000537:Aluminum Oxide
keywords: Cell morphology; Cell proliferation; MC3T3-E1; Stainless steel; Surface treatment