title: Phage-protease-peptide: a novel trifecta enabling multiplex detection of viable bacterial pathogens.
abstract: Bacteriophages represent rapid, readily targeted, and easily produced molecular probes for the detection of bacterial pathogens. Molecular biology techniques have allowed researchers to make significant advances in the bioengineering of bacteriophage to further improve speed and sensitivity of detection. Despite their host specificity, bacteriophages have not been meaningfully leveraged in multiplex detection of bacterial pathogens. We propose a proof-of-principal phage-based scheme to enable multiplex detection. Our scheme involves bioengineering bacteriophage to carry a gene for a specific protease, which is expressed during infection of the target cell. Upon lysis, the protease is released to cleave a reporter peptide, and the signal detected. Here we demonstrate the successful (i) modification of T7 bacteriophage to carry tobacco etch virus (TEV) protease; (ii) expression of TEV protease by Escherichia coli following infection by our modified T7, an average of 2000 units of protease per phage are produced during infection; and (iii) proof-of-principle detection of E. coli in 3 h after a primary enrichment via TEV protease activity using a fluorescent peptide and using a designed target peptide for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (MALDI-TOF MS) analysis. This proof-of-principle can be translated to other phage-protease-peptide combinations to enable multiplex bacterial detection and readily adopted on multiple platforms, like MALDI-TOF MS or fluorescent readers, commonly found in labs.
authors.affiliation: Department of Food Science, University of Massachusetts, 246 Chenoweth Laboratory, 102 Holdsworth Way, Amherst, MA, 01003, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Alcaine
authors.forename: L
authors.initials: L
authors.lastname: Tilton
authors.forename: M A C
authors.initials: MA
authors.lastname: Serrano
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: R W
authors.initials: RW
authors.lastname: Vachet
authors.forename: S R
authors.initials: SR
authors.lastname: Nugen
mesh_terms: D001434:Bacteriophage Typing / Q000379:methods*; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000821:virology*