title: Chronic treatment with Sildenafil has no effect on folliculogenesis or fertility in C57BL/6 and C57BL/6 knockout for iNOS mice.
abstract: Sildenafil is an important phosphodiesterase inhibitor used to treat a range of diseases, including cardiovascular disease, prostatic hyperplasia and pulmonary hypertension. Its main mechanism of action is the inhibition of phosphodiesterase 5, leading to increased intracellular cyclic guanosine 3',5'-monophosphate. This second messenger plays an interesting role in the reproductive tract. The aim of the present study was to evaluate the effect of Sildenafil on folliculogenesis and fertility in mice. To do so, C57BL/6 wild-type mice and inducible nitric oxide synthase knockout (iNOS(-/-)) mice were treated with Sildenafil, and reproductive variables were evaluated. The treated and control animals underwent estrous cycle and fertility assay. Lipid profile, serum nitric oxide levels and the expression of endothelial nitric oxide synthase, inducible nitric oxide synthase and guanylate cyclase were evaluated. Additionally, ovaries were submitted to histological and morphological analysis. The findings demonstrated that chronic treatment with Sildenafil had no effect on folliculogenesis or fertility in C57BL/6 mice, suggesting that this drug can be safely used by women of childbearing age.
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: maridonato@gmail.com.
authors.forename: Mariana Aragão Matos
authors.initials: MA
authors.lastname: Donato
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: edlenelimaribeiro@gmail.com.
authors.forename: Edlene Lima
authors.initials: EL
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: dileniacipriano@ig.com.br.
authors.forename: Dilênia de Oliveira Cipriano
authors.initials: Dde O
authors.lastname: Torres
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: kls.amanda@gmail.com.
authors.forename: Amanda Karolina
authors.initials: AK
authors.lastname: Soares E Silva
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: gomes.bio@gmail.com.
authors.forename: Fabiana Oliveira
authors.initials: FO
authors.lastname: Dos Santos Gomes
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: ssbruna@gmail.com.
authors.forename: Bruna
authors.initials: B
authors.lastname: Santos E Silva
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil. Electronic address: surawanessa@gmail.com.
authors.forename: Sura Wanessa Santos
authors.initials: SW
authors.lastname: Rocha
authors.affiliation: Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universitária, CEP: 50.670-420, Recife, Brazil; Laboratório de Microscopia e Microanálise, Centro de Tecnologias Estratégicas do Nordeste, Av. Prof°. Luiz Freire, 01 - Cidade Universitária, CEP: 50.740-540, Recife, Brazil. Electronic address: peixoto.christina@gmail.com.
authors.forename: Christina Alves
authors.initials: CA
authors.lastname: Peixoto
mesh_terms: D000818:Animals; D030762:Estrous Cycle / Q000187:drug effects / Q000502:physiology; D005298:Fertility / Q000187:drug effects*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects* / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000187:drug effects*; D000068677:Sildenafil Citrate / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D009569:Nitric Oxide; D000068677:Sildenafil Citrate; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III
keywords: Ovary; PDE5; Sildenafil