title: Leaf and root glucosinolate profiles of Chinese cabbage (Brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation.
abstract: Glucosinolates (GSs) are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid (JA) and salicylic acid (SA) are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage (Brassica rapa ssp. pekinensis). In this study, we applied different concentrations of methyl jasmonate (MeJA) and/or SA onto the leaf and root of Chinese cabbage to investigate the time-course interactive profiles of GSs. Regardless of the site of the elicitation and the concentrations of the elicitors, the roots accumulated much more GSs and were more sensitive and more rapidly responsive to the elicitors than leaves. Irrespective of the elicitation site, MeJA had a greater inducing and longer lasting effect on GS accumulation than SA. All three components of indole GS (IGS) were detected along with aliphatic and aromatic GSs. However, IGS was a major component of total GSs that accumulated rapidly in both root and leaf tissues in response to MeJA and SA elicitation. Neoglucobrassicin (neoGBC) did not respond to SA but to MeJA in leaf tissue, while it responded to both SA and MeJA in root tissue. Conversion of glucobrassicin (GBC) to neoGBC occurred at a steady rate over 3 d of elicitation. Increased accumulation of 4-methoxy glucobrassicin (4-MGBC) occurred only in the root irrespective of the type of elicitors and the site of elicitation. Thus, accumulation of IGS is a major metabolic hallmark of SA- and MeJA-mediated systemic response systems. SA exerted an antagonistic effect on the MeJA-induced root GSs irrespective of the site of elicitation. However, SA showed synergistic and antagonistic effects on the MeJA-induced leaf GSs when roots and leaves are elicitated for 3 d, respectively.
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China.
authors.forename: Yun-xiang
authors.initials: YX
authors.lastname: Zang
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China.
authors.forename: Jia-li
authors.initials: JL
authors.lastname: Ge
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China.
authors.forename: Ling-hui
authors.initials: LH
authors.lastname: Huang
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China.
authors.forename: Xi-shan
authors.initials: XS
authors.lastname: Lv
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China; zhengww@zafu.edu.cn.
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Zheng
authors.affiliation: Department of Biotechnology, University of Houston-Clear Lake, Houston, TX 77058-1098, USA.
authors.forename: Seung-beom
authors.initials: SB
authors.lastname: Hong
authors.affiliation: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Research Center of Bio-Breeding Industry, School of Agricultural and Food Science, Zhejiang A & F University, Lin'an 311300, China; zhuzj@zafu.edu.cn.
authors.forename: Zhu-jun
authors.initials: ZJ
authors.lastname: Zhu
mesh_terms: D000085:Acetates / Q000008:administration & dosage*; D029741:Brassica rapa / Q000187:drug effects / Q000378:metabolism*; D003517:Cyclopentanes / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005961:Glucosinolates / Q000378:metabolism*; D008657:Metabolic Clearance Rate / Q000187:drug effects / Q000502:physiology; D054883:Oxylipins / Q000008:administration & dosage*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D020156:Salicylic Acid / Q000008:administration & dosage*; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005961:Glucosinolates; D054883:Oxylipins; C072239:methyl jasmonate; D020156:Salicylic Acid
keywords: Chinese cabbage; Glucosinolate; Interactive effect; Methyl jasmonate; Salicylic acid