title: Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model.
abstract: Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a control group. The results showed that the hTERT-bMGEs group had much higher transgenic efficiency and protein expression than the bMGEs group. Furthermore, the nontransgenic and transgenic hTERT-bMGEs were used as donor cells to evaluate the efficiency of SCNT. There were no significant differences in rates of cleavage or blastocysts or hatched blastocysts of cloned embryos from nontransgenic hTERT-bMGEs at passage 18 and 28 groups (82.8% vs. 81.9%, 28.6% vs. 24.8%, 58.6% vs. 55.3%, respectively) and the transgenic group (80.8%, 26.5% and 53.4%); however, they were significantly higher than the bMGEs group (71.2%, 12.8% and 14.8%), (p < 0.05). We confirmed that hTERT-bMGEs could serve as effective target cells for improving development of somatic cell cloned cattle embryos.
authors.affiliation: School of Life Science and Technology, Inner Mongolia University of Science & Technology, Baotou 014010, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: He
authors.affiliation: School of Life Science and Technology, Inner Mongolia University of Science & Technology, Baotou 014010, China.
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Ma
authors.affiliation: Research and Development Center for Tissue Engineering, Xi'an 710048, China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: He
authors.affiliation: School of Life Science and Technology, Inner Mongolia University of Science & Technology, Baotou 014010, China.
authors.forename: Wan-Tong
authors.initials: WT
authors.lastname: Si
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Shenyang 712100, China.|Key Laboratory of Animal Reproductive Endocrinology & Embryo Engineering, Ministry of Agriculture, Shenyang 712100, China.
authors.forename: Yue-Mao
authors.initials: YM
authors.lastname: Zheng
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D019976:Cloning, Organism / Q000662:veterinary; D047108:Embryonic Development; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D009113:Muramidase / Q000235:genetics* / Q000378:metabolism; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000662:veterinary
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C509186:TERT protein, human; D019098:Telomerase; D009113:Muramidase
keywords: bovine; human telomerase reverse transcriptase; mammary gland epithelial cells; somatic cell nuclear transfer