title: Identification of autophagy signaling network that contributes to stroke in the ischemic rodent brain via gene expression.
abstract: Autophagy plays a vital role in cerebral ischemia and may be a potential target for developing novel therapy for stroke. In this study, we constructed an autophagy-related pathway network by analyzing the genes related to autophagy and ischemic stroke, and the risk genes were screened. Two autophagy-related modules were significantly up-regulated and clustered to influence cerebral ischemia. Besides, three key modular genes (NFKB1, RELA, and STAT3) were revealed. With 5-fold cross validation, the ROC curves of NFKB1, RELA, and STAT3 were 0.8256, 0.8462, and 0.8923. They formed a complex module and competitively mediated the activation of autophagy in cerebral ischemia. In conclusion, a module containing NFKB1, RELA, and STAT3 mediates autophagy, serving as a potential biomarker for the diagnosis and therapy of ischemic stroke.
authors.affiliation: Department of Vascular Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Jinyun
authors.initials: J
authors.lastname: Tan
authors.forename: Weihao
authors.initials: W
authors.lastname: Shi
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000235:genetics*; D015870:Gene Expression; D053263:Gene Regulatory Networks; D006801:Humans; D012372:ROC Curve; D015398:Signal Transduction / Q000235:genetics; D020521:Stroke / Q000235:genetics*