title: Serum Samples From Middle-aged Adults Vaccinated Annually with Seasonal Influenza Vaccines Cross-neutralize Some Potential Pandemic Influenza Viruses.
abstract: We examined serum samples from adults ages 48-64 who received multiple seasonal influenza vaccines from 2004 to 2009 for cross-neutralizing antibodies to potential pandemic strains. Using pseudoviruses bearing various hemagglutinins (HA-pseudoviruses), we found serum neutralization titers (≥160) in 100% against A/Japan/305/1957 (H2N2), 53% against A/Hong Kong/1073/99 (H9N2), 56% against the H3N2 variant A/Indiana/08/11 (H3N2v), 11% against A/Hong Kong/G9/97 (H9N2), and 36% A/chicken/Hong Kong/SF4/01 (H6N1). None had titers >160 to A/Shanghai/2/13 (H7N9) or A/Netherlands/219/03 (H7N7). Thirty-six percent to 0% had neutralization titers to various H5N1 strains. Titers to H9, H6, and H5 HA-pseudoviruses correlated with each other, but not with H3N2v, suggesting group-specific cross-neutralization.
authors.affiliation: Laboratory of Immunoregulation, Division of Viral Products.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Laboratory of Immunoregulation, Division of Viral Products.
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Alvarado-Facundo
authors.affiliation: Laboratory of Immunoregulation, Division of Viral Products.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Division of Hematology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Anderson
authors.affiliation: Division of Hematology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Scott
authors.affiliation: Laboratory of Immunoregulation, Division of Viral Products.
authors.forename: Russell
authors.initials: R
authors.lastname: Vassell
authors.affiliation: Laboratory of Immunoregulation, Division of Viral Products.
authors.forename: Carol D
authors.initials: CD
authors.lastname: Weiss
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007251:Influenza, Human / Q000097:blood / Q000517:prevention & control* / Q000821:virology*; D008875:Middle Aged; D058873:Pandemics
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines
keywords: cross-neutralization; influenza vaccines; neutralizing antibodies; pandemic influenza; stem antibodies