title: Effect of combined microbes on plant tolerance to Zn-Pb contaminations.
abstract: The presence and composition of soil microbial communities has been shown to have a large impact on plant-plant interactions and consequently plant diversity and composition. The goal of the present study was to evaluate impact of arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing bacteria, which constitutes an essential link between the soil and the plant's roots. A greenhouse pot experiment was conducted to evaluate the feasibility of using selected microbes to improve Hieracium pilosella and Medicago sativa growth on Zn-Pb-rich site. Results of studies revealed that biomass, the dry mass of shoots and roots, increased significantly when plants were inoculated with mycorrhizal fungi and nitrogen-fixing bacteria. The addition of Azospirillum sp. and Nostoc edaphicum without mycorrhiza suppressed plant growth. Single bacterial inoculation alone does not have a positive effect on M. sativa growth, while co-inoculation with AMF improved plant growth. Plant vitality (expressed by the performance index) was improved by the addition of microbes. However, our results indicated that even dry heat sterilization of the substratum created imbalanced relationships between soil-plant and plants and associated microorganisms. The studies indicated that AMF and N2-fixers can improve revegetation of heavy metal-rich industrial sites, if the selection of interacting symbionts is properly conducted.
authors.affiliation: Plant-Microbial Interaction Research Group, Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland. anna.ogar@uj.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Ogar
authors.affiliation: Ecosystem Ecology Research Group, Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland. lukasz.sobczyk@uj.edu.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Sobczyk
authors.affiliation: Plant-Microbial Interaction Research Group, Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland. katarzyna.turnau@uj.edu.pl.|The Malopolska Center of Biotechnology, Jagiellonian University, Krakow, Poland. katarzyna.turnau@uj.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Turnau
mesh_terms: D000383:Agriculture; D019659:Asteraceae / Q000187:drug effects / Q000254:growth & development* / Q000382:microbiology; D000458:Cyanobacteria / Q000187:drug effects / Q000254:growth & development; D007854:Lead / Q000494:pharmacology*; D000455:Medicago sativa / Q000187:drug effects / Q000254:growth & development* / Q000382:microbiology; D038821:Mycorrhizae / Q000187:drug effects / Q000254:growth & development; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology*; D013559:Symbiosis; D015032:Zinc / Q000494:pharmacology*
chemical_list: D012987:Soil; D012989:Soil Pollutants; D007854:Lead; D015032:Zinc
keywords: Arbuscular mycorrhizal fungi (AMF); Co-cropping; Cyanobacteria; N2-fixing bacteria; Plant vitality; Plant/microbial interactions