title: False-negative BD MGIT™ TBc Identification Test results in routine tuberculosis diagnosis: a New Zealand perspective.
abstract: >We previously reported on a comparison of the AccuProbe(®) Gen-Probe(®) MTBC assay (AccuProbe) (BioMérieux, Marcy L'Etoile, France) with the Becton Dickinson (BD) MGIT™ TBc Identification (TBc) Test (BD, Franklin Lakes, NJ, USA) in our laboratory. In the period following the shift from the AccuProbe assay to the TBc test, we obtained six false-negative results. On sequencing the mpt64 gene, we found that these false-negative cases had mutations in the mpt64 gene due to deletion, insertion or substitution. Despite the occurrence of false-negative results, we found that the reduced cost and minimal technical expertise, combined with a new testing algorithm, still make this test the preferred option for rapidly identifying Mycobacterium tuberculosis complex in MGIT cultures in a low TB burden country such as New Zealand.
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: I
authors.initials: I
authors.lastname: Basu
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: J E
authors.initials: JE
authors.lastname: Bower
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: G K
authors.initials: GK
authors.lastname: Henderson
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: O
authors.initials: O
authors.lastname: Lowe
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: S
authors.initials: S
authors.lastname: Newton
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: R
authors.initials: R
authors.lastname: Vaughan
authors.affiliation: Clinical Microbiology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
authors.forename: S A
authors.initials: SA
authors.lastname: Roberts
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D004252:DNA Mutational Analysis; D005188:False Negative Reactions; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009520:New Zealand; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology*; D055815:Young Adult