title: Evaluation of the Ideal Implant Insertion Time in Human Bone Biopsies After Sinus Elevation Using a Combination of Autologous Bone and Graft Substitute.
abstract: OBJECTIVE
  To evaluate the ideal implant insertion time in human bone biopsies after sinus elevation with a composite graft consisting of an equal amount of biomaterial and autologous bone, by comparing the bone regeneration obtained 4 to 5 months after surgery with that obtained after 6 to 8 months, and using the adjacent native bone as reference.
  
  
  METHODS
  Twenty-six biopsy specimens of 11 patients were analyzed. Two groups were created depending on the time of implant insertion: group t1 at 4 to 5 months (n = 13) and group t2 at 6 to 8 months (n = 13). The same volume of grafted bone and native bone were analyzed for each biopsy with microcomputed tomography (microCT) and gene expression analysis.
  
  
  RESULTS
  Statistically significant differences were found in bone mineral density (BMD), bone volume fraction, and trabecular separation (TbSp) between native and grafted bone in both groups, with higher grafted bone values, except for the variable TbSp, which was lower in the grafted bone. This decrease in TbSp in the grafted bone in both groups can be explained by the significant increase in trabecular thickness in group t2 and the trabecular number in group t1, compared with native bone. No significant differences were found between the two groups in the morphometric parameters and BMD of the grafted bone. Also, no significant changes in the messenger RNA (mRNA) levels of bone formation, bone resorption, and inflammatory markers were found between both groups, with the exception that alkaline phosphatase mRNA levels were significantly lower in group t1 relative to native bone.
  
  
  CONCLUSIONS
  This composite graft showed no differences in three-dimensional microstructure, BMD, or at the molecular level between 4 to 5 months and 6 to 8 months of healing time. Thus, this time can be shortened to 4 months with the security of a grafted area of mature bone.
authors.forename: Mikel
authors.initials: M
authors.lastname: Ramos-Murguialday
authors.forename: Jorge
authors.initials: J
authors.lastname: Caubet
authors.forename: Joana Maria
authors.initials: JM
authors.lastname: Ramis
authors.forename: Marta
authors.initials: M
authors.lastname: Monjo
mesh_terms: D064592:Autografts / Q000473:pathology / Q000637:transplantation*; D001706:Biopsy / Q000379:methods; D015519:Bone Density / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology; D001862:Bone Resorption / Q000503:physiopathology; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods*; D003758:Dental Implantation, Endosseous / Q000379:methods*; D015921:Dental Implants; D005500:Follow-Up Studies; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D008443:Maxillary Sinus / Q000473:pathology; D009929:Organ Size; D010012:Osteogenesis / Q000502:physiology; D059546:Sinus Floor Augmentation / Q000379:methods*; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D018786:Bone Substitutes; D015921:Dental Implants