title: Adenosquamous carcinoma of the head and neck: Molecular analysis using CRTC-MAML FISH and survival comparison with paired conventional squamous cell carcinoma.
abstract: OBJECTIVE
  Adenosquamous carcinoma (ASC) is a rare variant of head-and-neck squamous cell carcinoma (HNSCC) generally thought to be uniformly aggressive with poor prognosis. However, it remains unknown how overall survival compare with conventional HNSCC. Here we report for the first time that ASC does not necessarily indicate a worse prognosis than conventional HNSCC.
  
  
  METHODS
  Case-control retrospective study.
  
  
  METHODS
  Forty-two primary tumors of the head and neck, treated with curative intent, were identified. Next, 2:1 matching of HNSCC was performed using the following matching criteria: gender, site, pathologic tumor stage, and pathologic node stage. Successful matching was performed for 32 of 42 tumors. Additionally, 20 samples were sent for break-apart FISH testing to evaluate for the presence of the CRTC1-MAML2 translocation.
  
  
  RESULTS
  There was a 1.8:1 male to female ratio, with a mean age of 62 years (range 38-84). The layrnx was the most common site (26%), followed by oropharynx (24%), oral cavity (19%), and sinonasal (17%). Kaplan-Meier analysis of adenosquamous and matched HNSCC showed similar survival curves. Median survival times for ASC and HNSCC were 4 and 6 years, respectively. A random-effects Cox model with Gamma frailty revealed no statistical difference between the two groups (P=0.25). All cases of ASC were negative for the CRTC1-MAML2 translocation.
  
  
  CONCLUSIONS
  This study directly compares primary ASC with HNSCC. No difference in overall survival was detected in contradistinction to the previously thought uniformly poor prognosis. We also highlight the importance of the CRTC1-MAML2 translocation in distinguishing ASC from mucoepidermoid carcinoma.
  
  
  METHODS
  3b.
authors.affiliation: Department of Otolaryngology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.
authors.forename: Jason I
authors.initials: JI
authors.lastname: Kass
authors.affiliation: Department of Otolaryngology, Loma Linda Medical Center, Loma Linda, California, U.S.A.
authors.forename: Steve C
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Department of Biostatistics, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania.
authors.forename: Shira
authors.initials: S
authors.lastname: Abberbock
authors.affiliation: Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.
authors.forename: Raja R
authors.initials: RR
authors.lastname: Seethala
authors.affiliation: Department of Otolaryngology, VA Pittsburgh Health System, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.
authors.forename: Umamaheswar
authors.initials: U
authors.lastname: Duvvuri
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018196:Carcinoma, Adenosquamous / Q000401:mortality*; D002294:Carcinoma, Squamous Cell / Q000401:mortality; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D006258:Head and Neck Neoplasms / Q000401:mortality*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000401:mortality; D009687:Nuclear Proteins / Q000235:genetics; D009959:Oropharyngeal Neoplasms / Q000401:mortality; D011379:Prognosis; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: C500984:CRTC1 protein, human; D004268:DNA-Binding Proteins; C471297:MAML2 protein, human; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors
keywords: Adenosquamous; CRCT-MAML; FISH; carcinoma