title: Production and characterization of poly-3-hydroxybutyrate from Bacillus cereus PS 10.
abstract: Usage of renewable raw materials for production of fully degradable bioplastics (bacterial poly-3-hydroxybutyrate, PHB) has gained immense research impetus considering recalcitrant nature of petroleum based plastics, dwindling fossil fuel feed stocks, and associated green house gas emissions. However, high production cost of PHB is the major bottleneck for its wide range industrial applications. In current study, Bacillus cereus PS 10, a recent isolate, efficiently utilized molasses, an abundantly available by-product from sugar industries as sole carbon source for growth and PHB production. Most influential bioprocess variables i.e. molasses, pH and NH4Cl were identified based on Plackett-Burman-designed experiments. Design of experiment approach (response surface methodology) was further employed for optimization of these bioprocess variables, and an enhanced PHB yield (57.5%) was obtained. PHB produced by Bacillus cereus PS 10 was investigated using various physico-chemical approaches viz. thermogravimetric analysis, proton and carbon NMR ((1)H and (13)C) spectroscopy, melting point, elemental analysis and polarimetry for its detail characterization, and assessment for industrial application potential.
authors.affiliation: School of Biotechnology, University of Jammu, New Campus, BAWE-WALI RAKH, Jammu 180006, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sharma
authors.affiliation: School of Biotechnology, University of Jammu, New Campus, BAWE-WALI RAKH, Jammu 180006, India. Electronic address: bajajbijenderk@gmail.com.
authors.forename: Bijender Kumar
authors.initials: BK
authors.lastname: Bajaj
mesh_terms: D001409:Bacillus cereus / Q000378:metabolism*; D005285:Fermentation; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism*; D013818:Thermogravimetry; D044366:Transition Temperature; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Bacillus cereus PS 10-poly-3-hydroxybutyrate; Design of experiment approach; PHB-characterization