title: Photoinduced Electron-Transfer-Based Hybridization Probes for Detection of DNA and RNA.
abstract: Here, we report the synthesis of a hybridization probe for detection of RNA and DNA based on photoinduced electron transfer (PeT). We designed and synthesized an oligonucleotide containing an adenosine analogue with a 9-(N,N-dimethylaminomethyl)anthracenyl moiety at its 6-position via an ethynylene linker as the hybridization probe. When the probe was hybridized with a complementary RNA or DNA, the fluorescence intensity increased 3-fold or 4.5-fold, respectively, compared to the single-stranded state.
authors.forename: Kan
authors.initials: K
authors.lastname: Takahashi
authors.forename: Shun
authors.initials: S
authors.lastname: Ito
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nakamoto
authors.affiliation: Division for Medical Research Engineering, Nagoya University Graduate School of Medicine , Nagoya 466-8550, Japan.
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Ito
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D000873:Anthracenes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004579:Electron Transport; D004583:Electrons; D005453:Fluorescence; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
chemical_list: D000873:Anthracenes; D015342:DNA Probes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D000241:Adenosine