title: Neural crest specification by Prohibitin1 depends on transcriptional regulation of prl3 and vangl1.
abstract: A complex network of transcription factors regulates specification of neural crest cells at early neurula stage by stabilizing neural crest identity and activating neural crest effector genes so that distinct subpopulations evolve. In this network, c-myc acts on top of the gene hierarchy controlling snail2, AP2 and prohibitin1 (phb1) expression. While snail2 and AP2 are well studied neural crest specifier genes little is known about the role of phb1 in this process. To identify phb1 regulated genes we analyzed the transcriptome of neural crest explants of phb1 morphant Xenopus embryos. Among 147 phb1 regulated genes we identified the membrane-associated protein-tyrosine phosphatase PRP4A3 (prl3) and the atypical cadherin and Wnt-PCP component van gogh like1 (vangl1). Gain of function, loss of function and epistasis experiments allowed us to allocate both genes in the neural crest specification network between phb1 and twist. Interestingly, both, vangl1 and prl3 regulate only a small subset of neural crest marker genes. The identification of two membrane-associated proteins as novel neural crest specifiers indicates that in addition to gene regulation by combinatory effects of transcription factors also post-translational modifications (prl3) and cell-cell adhesion and/or regulation of cell-polarity (vangl1) specify the identity of neural crest cell populations. genesis 53:627-639, 2015. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Cell and Developmental Biology, KIT, Campus South, Zoological Institute, Karlsruhe, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Deichmann
authors.affiliation: Department of Cell and Developmental Biology, KIT, Campus South, Zoological Institute, Karlsruhe, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Link
authors.affiliation: Department of Cell and Developmental Biology, KIT, Campus South, Zoological Institute, Karlsruhe, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Seyfang
authors.affiliation: Department of Cell and Developmental Biology, KIT, Campus South, Zoological Institute, Karlsruhe, Germany.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Knotz
authors.affiliation: Department of Cell and Developmental Biology, KIT, Campus South, Zoological Institute, Karlsruhe, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Gradl
authors.affiliation: Department of Cell and Developmental Biology, KIT, Campus South, Zoological Institute, Karlsruhe, Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Wedlich
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019521:Body Patterning / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009432:Neural Crest / Q000196:embryology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D000091402:Prohibitins; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D000091402:Prohibitins; D012097:Repressor Proteins; D051879:Twist-Related Protein 1; D029867:Xenopus Proteins; C000717336:prohibitin 1 protein, Xenopus; C000607039:vangl1 protein, Xenopus; C000592341:PTP4A3 protein, Xenopus; D017027:Protein Tyrosine Phosphatases
keywords: amphibian; early development; neural crest; patterning; signaling