title: Duplex real-time PCR method for the detection of sesame (Sesamum indicum) and flaxseed (Linum usitatissimum) DNA in processed food products.
abstract: The development of a duplex real-time polymerase chain reaction (PCR) method allowing the simultaneous detection of sesame and flaxseed DNA in commercial food products is described. This duplex real-time PCR technique is based in the design of sesame- and flaxseed-specific primers based on the ITS1 region and two TaqMan fluorescent probes. The method was positive for sesame and flaxseed, and showed no cross-reactivity for all other heterologous plant and animal species tested. Sesame and flaxseed could be detected in a series of model samples with defined raw and heat-treated sesame in flaxseed, and flaxseed in sesame, respectively, with detection limits of 1.3 mg kg(-1) for sesame and 1.4 mg kg(-1) for flaxseed. The applicability of the assay for determining sesame and flaxseed in different food matrices was investigated by analysing a total of 238 commercial foodstuffs. This PCR method is useful for highly selective and sensitive detection of traces of sesame and flaxseed in commercial food products.
authors.affiliation: a Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria , Universidad Complutense de Madrid , Madrid , Spain.
authors.forename: Inés María
authors.initials: IM
authors.lastname: López-Calleja
authors.affiliation: a Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria , Universidad Complutense de Madrid , Madrid , Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: de la Cruz
authors.affiliation: a Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria , Universidad Complutense de Madrid , Madrid , Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Martín
authors.affiliation: a Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria , Universidad Complutense de Madrid , Madrid , Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: González
authors.affiliation: a Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria , Universidad Complutense de Madrid , Madrid , Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: García
mesh_terms: D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics*; D019597:Flax / Q000235:genetics*; D005504:Food Analysis / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D031685:Sesamum / Q000235:genetics*
chemical_list: D018744:DNA, Plant
keywords: commercial food products; duplex TaqMan real-time PCR; flaxseed (Linum usitatissimum); food allergy; sesame (Sesamum indicum)