title: Catecholate-siderophore produced by As-resistant bacterium effectively dissolved FeAsO4 and promoted Pteris vittata growth.
abstract: The impact of siderophore produced by arsenic-resistant bacterium Pseudomonas PG12 on FeAsO4 dissolution and plant growth were examined. Arsenic-hyperaccumulator Pteris vittata was grown for 7 d in 0.2-strength Fe-free Hoagland solution containing FeAsO4 mineral and PG12-siderophore or fungal-siderophore desferrioxamine B (DFOB). Standard siderophore assays indicated that PG12-siderophore was catecholate-type. PG12-siderophore was more effective in promoting FeAsO4 dissolution, and Fe and As plant uptake than DFOB. Media soluble Fe and As in PG12 treatment were 34.6 and 3.07 μM, 1.6- and 1.4-fold of that in DFOB. Plant Fe content increased from 2.93 to 6.24 g kg(-1) in the roots and As content increased from 14.3 to 78.5 mg kg(-1) in the fronds. Besides, P. vittata in PG12 treatment showed 2.6-times greater biomass than DFOB. While P. vittata fronds in PG12 treatment were dominated by AsIII, those in DFOB treatment were dominated by AsV (61-77%). This study showed that siderophore-producing arsenic-resistant rhizobacteria may have potential in enhancing phytoremediation of arsenic-contaminated soils.
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China.
authors.forename: Guang-Mei
authors.initials: GM
authors.lastname: Yang
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China.
authors.forename: Dong-Xing
authors.initials: DX
authors.lastname: Guan
authors.affiliation: Soil and Water Science Department, University of Florida, Gainesville, FL 32611, United States.
authors.forename: Piyasa
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, United States. Electronic address: lqma@ufl.edu.
authors.forename: Lena Q
authors.initials: LQ
authors.lastname: Ma
mesh_terms: D001152:Arsenicals / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D003676:Deferoxamine / Q000737:chemistry*; D024881:Drug Resistance, Bacterial; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D032495:Pteris / Q000254:growth & development* / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012995:Solubility
chemical_list: D001152:Arsenicals; D012989:Soil Pollutants; C548094:scorodite; D003676:Deferoxamine
keywords: Arsenic; Phytoremediation; Pseudomonas; Pteris vittata; Siderophore