title: Solid-state NMR: An emerging technique in structural biology of self-assemblies.
abstract: Protein self-assemblies are ubiquitous biological systems involved in many cellular processes, ranging from bacterial and viral infection to the propagation of neurodegenerative disorders. Studying the atomic three-dimensional structures of protein self-assemblies is a particularly demanding task, as these systems are usually insoluble, non-crystalline and of large size. Solid-state NMR (ssNMR) is an emerging method that can provide atomic-level structural data on intact macromolecular assemblies. We here present recent progress in magic-angle spinning ssNMR to study protein assemblies and give an overview on its combination with complementary techniques such as cryo-EM, mass-per-length measurements, SAXS and X-ray diffraction. Applications of ssNMR on its own and in hybrid approaches have revealed precious atomic details and first high-resolution structures of complex biological assemblies, including amyloid fibrils, bacterial filaments, phages or virus capsids.
authors.affiliation: Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), CNRS, Universite Bordeaux, Institut Polytechnique Bordeaux, All. Geoffroy Saint-Hilaire, 33600 Pessac, France. Electronic address: b.habenstein@iecb.u-bordeaux.fr.
authors.forename: Birgit
authors.initials: B
authors.lastname: Habenstein
authors.affiliation: Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), CNRS, Universite Bordeaux, Institut Polytechnique Bordeaux, All. Geoffroy Saint-Hilaire, 33600 Pessac, France. Electronic address: a.loquet@iecb.u-bordeaux.fr.
authors.forename: Antoine
authors.initials: A
authors.lastname: Loquet
mesh_terms: D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins
keywords: Amyloid fibril; Bacterial filament; Hybrid structure determination; Protein supramolecular assembly; Solid-state NMR spectroscopy