title: The role of carrier ligands of platinum(II) anticancer complexes in the protein recognition of Pt-DNA adducts.
abstract: In order to systematically investigate the influence of carrier ligands on the interaction of Pt-DNA adducts with damage recognition proteins, a series of DNA probes containing 1,2-GG platinum compound crosslinks using cisplatin, oxaliplatin, (S,S-DACH)PtCl2 and (cis-1,4-DACH)PtCl2 (kiteplatin) has been constructed. These complexes share similar DNA binding properties although they exhibit quite different cytotoxicity. It is revealed that HMGB1 (high-mobility group protein B1) was the most commonly found protein that recognizes all Pt(II)-DNA probes and prefers cisplatin-DNA probes more than the others. Interestingly, an important component of the replication protein A complex, RPA2, was found to bind to kiteplatin much more tightly than other proteins. These results may be important for the interpretation of the roles of carrier ligands in platinum(II)-based anticancer complexes.
authors.affiliation: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Collaborative Innovation Centre of Chemistry for Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, 210093, P. R. China. chenhao@nju.edu.cn zguo@nju.edu.cn.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Qiao
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Weizhong
authors.initials: W
authors.lastname: Chen
authors.forename: Xichun
authors.initials: X
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Zijian
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D002945:Cisplatin / Q000008:administration & dosage / Q000737:chemistry*; D003514:Cyclohexylamines / Q000737:chemistry; D018736:DNA Adducts / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D008024:Ligands; D009944:Organoplatinum Compounds / Q000008:administration & dosage / Q000737:chemistry*; D000077150:Oxaliplatin
chemical_list: D000970:Antineoplastic Agents; D003514:Cyclohexylamines; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D004337:Drug Carriers; D008024:Ligands; D009944:Organoplatinum Compounds; C445102:platinum(II)(1,4-diazacycloheptane)dichloride; D000077150:Oxaliplatin; C030435:1,2-cyclohexanediamine; D002945:Cisplatin