title: Transiently responsive protein-polymer conjugates via a 'grafting-from' RAFT approach for intracellular co-delivery of proteins and immune-modulators.
abstract: We report on transiently responsive protein-polymer conjugates that temporarily change their protein conformation from the soluble to the particle-like state. 'Grafting-from' RAFT polymerization of a dioxolane-containing acrylamide with a protein macroCTA is used to design polymer-protein conjugates that self-assemble into nanoparticles at physiological temperature and pH. Acid triggered hydrolysis of the dioxolane units into diol moeities rendered the conjugates fully water soluble irrespective of temperature.
authors.affiliation: Department of Pharmaceutics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. br.degeest@ugent.be.
authors.forename: N
authors.initials: N
authors.lastname: Vanparijs
authors.forename: R
authors.initials: R
authors.lastname: De Coen
authors.forename: D
authors.initials: D
authors.lastname: Laplace
authors.forename: B
authors.initials: B
authors.lastname: Louage
authors.forename: S
authors.initials: S
authors.lastname: Maji
authors.forename: L
authors.initials: L
authors.lastname: Lybaert
authors.forename: R
authors.initials: R
authors.lastname: Hoogenboom
authors.forename: B G
authors.initials: BG
authors.lastname: De Geest
mesh_terms: D000178:Acrylamides / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000378:metabolism; D004148:Dioxolanes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007155:Immunologic Factors / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*; D011487:Protein Conformation; D011804:Quinolines / Q000008:administration & dosage / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry*; D012995:Solubility; D013696:Temperature; D013844:Thiazoles / Q000008:administration & dosage / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; C551788:CL 075; D004148:Dioxolanes; D007155:Immunologic Factors; D011108:Polymers; D011804:Quinolines; D013844:Thiazoles; C551656:poly-N-((2,2-dimethyl-1,3-dioxolane)methyl)acrylamide; D014867:Water; D012710:Serum Albumin, Bovine; C010962:formal glycol