title: UBE2S is associated with malignant characteristics of breast cancer cells.
abstract: Ubiquitination is essential for various biological processes, such as signal transduction, intracellular trafficking, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskeleton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Akter Khondker
authors.initials: AK
authors.lastname: Ayesha
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Hyodo
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Asano
authors.affiliation: Department of Surgical Oncology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sato
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Mansour
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Ito
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan.
authors.forename: Michinari
authors.initials: M
authors.lastname: Hamaguchi
authors.affiliation: Division of Cancer Biology, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan. tsenga@med.nagoya-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senga
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000368:Aged; D023102:Anoikis; D001943:Breast Neoplasms / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism; D061986:MCF-7 Cells; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D016023:Integrins; D034741:RNA, Small Interfering; C544962:Ube2S protein, human; D044763:Ubiquitin-Conjugating Enzymes; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: Anoikis; Breast cancer; FAK; Invasion; Migration; UBE2S; Ubiquitination