title: Developmentally determined reduction in CD31 during gestation is associated with CD8+ T cell effector differentiation in preterm infants.
abstract: Homeostatic T cell proliferation is more robust during human fetal development. In order to understand the relative effect of normal fetal homeostasis and perinatal exposures on CD8+ T cell behavior in PT infants, we characterized umbilical cord blood CD8+ T cells from infants born between 23-42weeks gestation. Subjects were recruited as part of the NHLBI-sponsored Prematurity and Respiratory Outcomes Program. Cord blood from PT infants had fewer naïve CD8+ T cells and lower regulatory CD31 expression on both naïve and effector, independent of prenatal exposures. CD8+ T cell in vitro effector function was greater at younger gestational ages, an effect that was exaggerated in infants with prior inflammatory exposures. These results suggest that CD8+ T cells earlier in gestation have loss of regulatory co-receptor CD31 and greater effector differentiation, which may place PT neonates at unique risk for CD8+ T cell-mediated inflammation and impaired T cell memory formation.
authors.affiliation: University of Rochester, Department of Pediatrics, 601 Elmwood Avenue, Rochester, NY 14642, United States. Electronic address: kristin_scheible@urmc.rochester.edu.
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Scheible
authors.affiliation: University of Rochester, Department of Pediatrics, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Jason
authors.initials: J
authors.lastname: Emo
authors.affiliation: University of Rochester, Department of Biostatistics and Computational Biology, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yang
authors.affiliation: University of Rochester, Department of Biostatistics and Computational Biology, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Holden-Wiltse
authors.affiliation: University of Rochester, Department of Biostatistics and Computational Biology, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Andrew
authors.initials: A
authors.lastname: Straw
authors.affiliation: University of Rochester, Department of Pediatrics, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Heidie
authors.initials: H
authors.lastname: Huyck
authors.affiliation: University of Rochester, Department of Pediatrics, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Sara
authors.initials: S
authors.lastname: Misra
authors.affiliation: University of Rochester, Department of Microbiology and Immunology, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: David J
authors.initials: DJ
authors.lastname: Topham
authors.affiliation: Medical University of South Carolina, Department of Pediatrics, Rutledge Tower, 135 Rutledge Avenue, Charleston, SC 29425, United States.
authors.forename: Rita M
authors.initials: RM
authors.lastname: Ryan
authors.affiliation: State University of New York at Buffalo, Department of Pediatrics, 219 Bryant Street, Buffalo, NY 14222, United States.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Reynolds
authors.affiliation: University of Rochester, Department of Pediatrics, 601 Elmwood Avenue, Rochester, NY 14642, United States; University of Rochester, Pediatric Molecular and Personalized Medicine Program, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Mariani
authors.affiliation: University of Rochester, Department of Pediatrics, 601 Elmwood Avenue, Rochester, NY 14642, United States.
authors.forename: Gloria S
authors.initials: GS
authors.lastname: Pryhuber
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology*; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006706:Homeostasis / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D007231:Infant, Newborn; D007234:Infant, Premature / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology*; D011247:Pregnancy
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Bronchopulmonary dysplasia; CD8+ T cell; Fetus; Immune dysregulation; Inflammation; Neonatal immunity; Prematurity