title: One probe, two-channel imaging of nuclear and cytosolic compartments with orange and red emissive dyes.
abstract: Several new DNA-targeting probes that exhibit binding-induced 'turn on' fluorescence are presented. Two of the dyes, orange emissive 1, (E)-4-(4(-4-methylpiperazin-1-yl)phenyl)6-(4-(4-methylpi-perazin-1-yl)styryl)pyrimidin-2-ol), and red emissive 2, (E)-4-(4(-4-methyl-piperazin-1-yl)-phenyl)6-(4-(4-methylpiperazin-1-yl)styryl)-1,3-propanedionato-κO,κO']difluoroborane), are brightly fluorescent when bound to DNA, but are virtually non-fluorescent in aqueous solutions. Confocal fluorescence microscopy of live BT474, MCF7 and HEK293 cells demonstrates that both probes are cell permeable and rapidly accumulated intracellularly into cell nuclei and the cytosol. Taking advantage of their environmental sensitivity, these two pools of fluorophores are readily resolved into separate channels, and thus, a single dye allows two-color imaging of the nuclear and cytosolic compartments.
authors.affiliation: Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146, USA. jnwilson@miami.edu.
authors.forename: Demar R G
authors.initials: DR
authors.lastname: Pitter
authors.forename: Adrienne S
authors.initials: AS
authors.lastname: Brown
authors.forename: James D
authors.initials: JD
authors.lastname: Baker
authors.forename: James N
authors.initials: JN
authors.lastname: Wilson
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry*; D003600:Cytosol / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D015394:Molecular Structure
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes