title: Akt and cAMP response element binding protein mediate 17β-estradiol regulation of glucose transporter 3 expression in human SH-SY5Y neuroblastoma cell line.
abstract: Impaired glucose uptake is involved in Alzheimer's disease (AD) and glucose transporter 3 (Glut3) is the major neuronal glucose transporter. Estrogens contribute its theorized protective role against AD. The present studies aimed to examine the effect of 17β-estradiol (E2, the natural estrogen) on Glut3 expression and the underlying mechanisms by using human SH-SY5Y cell line. The results demonstrated that E2 increased Glut3 expression. E2 could stimulate the activation of Akt signaling pathway and the subsequent phosphorylation of cAMP response element binding protein (CREB). Akt/CREB pathway mediated E2-induced increase in Glut3 expression. These results suggested the mechanisms underlying E2-induced increase in Glut3 expression in human SH-SY5Y cell line and might provide the new data for elucidating the neuroprotective role of E2 against AD.
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226002 Jiangsu, China.
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Biochemistry & Molecular Biology, Medical College, Nantong University, Nantong, 226001 Jiangsu, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Life Science, Nantong University, Nantong, 226007 Jiangsu, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Xi si Road 20, Nantong, 226002 Jiangsu, China. Electronic address: lupingzhang1218@163.com.
authors.forename: Luping
authors.initials: L
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D004967:Estrogens; D051274:Glucose Transporter Type 3; C495687:SLC2A3 protein, human; D004958:Estradiol; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: 17β-estradiol; Akt; Alzheimer’s disease; Glucose transporter 3; Neuron; cAMP response element binding protein