title: Hydrocellular foam dressing increases the leptin level in wound fluid.
abstract: Hydrocellular foam dressing (HCF) absorbs excessive wound fluid, which contains various cytokines and growth factors, and ensures a moist environment to promote wound healing. However, the molecular mechanisms underlying the wound fluid component changes induced by HCF are poorly understood. In the present study, we examined the effect of HCF on wound healing and the associated regulatory mechanisms in relation to variations in cytokine levels in the wound fluid. We created full-thickness wounds on the dorsolateral skin of rats and collected the resulting wound fluid samples. HCF was immersed in a plate containing the wound fluids. HCF was then removed and the excess wound fluid remaining in the plate was examined by cytokine array and enzyme-linked immunosorbent assay. We also used a rat model and human dermal fibroblast cultures to examine the effect of wound fluid component changes during the wound healing process. Upon treatment with HCF, leptin levels were upregulated in the wound fluid. Fibroblast proliferation was enhanced and the effect was suppressed in the presence of leptin antagonist. In our in vivo model, HCF increased wound contraction compared with film dressings and this positive effect of HCF was suppressed by addition of leptin antagonist. Our results suggest that dermal fibroblast proliferation is upregulated by HCF due to increased leptin level at the wound surface, and these effects promote wound healing. We believe that the present study contributes to furthering the understanding of the mechanisms underlying the effects of HCF-induced wound healing.
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Sawako
authors.initials: S
authors.lastname: Yoshino
authors.affiliation: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Gojiro
authors.initials: G
authors.lastname: Nakagami
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ohira
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Kawasaki
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Shimura
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Iwatsuki
authors.affiliation: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sanada
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi-Hattori
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oishi
authors.affiliation: Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamane
mesh_terms: D000818:Animals; D047070:Bandages, Hydrocolloid; D001826:Body Fluids / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D020738:Leptin / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism / Q000473:pathology*; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D016207:Cytokines; D020738:Leptin
keywords: dermal fibroblast; leptin; wound fluid; wound healing