title: Type of Renal Replacement Therapy (Hemodialysis versus Peritoneal Dialysis) Does Not Affect Cytokine Gene Expression or Clinical Parameters of Renal Transplant Candidates.
abstract: Patients with renal failure suffer from immune disturbances, caused by uremic toxins and influenced by dialysis treatment. The aim of the present study was to reveal whether type of dialysis modality (hemodialysis, HD, versus peritoneal dialysis, PD) differentially affects the immunocompetence, particularly the expression of genes involved in the immune response.
  
  
  METHODS
  87 renal transplant candidates (66 HD, 21 PD) were included in the study.
  
  
  METHODS
  The peripheral blood RNA samples were obtained with the PAXgene Blood system just before transplantation. The gene expression of CASP3, FAS, TP53, FOXP3, IFNG, IL2, IL6, IL8, IL10, IL17, IL18, LCN2, TGFB1, and TNF was assessed with real-time PCR on custom-designed low density arrays (TaqMan). Gene expression data were analyzed in relation to pretransplant clinical parameters.
  
  
  RESULTS
  The mean expression of examined genes showed no significant differences between PD and HD with the exception of FAS, expression of which was 30% higher in PD patients compared to the HD group. There was nonsignificantly higher expression of proinflammatory cytokines in the PD group. The clinical inflammatory parameters (CRP, albumin, cholesterol, and hemoglobin levels) did not differ between the groups.
  
  
  CONCLUSIONS
  Type of renal replacement therapy exerts no differential effect on cytokine gene expression or inflammatory clinical parameters.
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kamińska
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kościelska-Kasprzak
authors.affiliation: Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Chudoba
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Oktawia
authors.initials: O
authors.lastname: Mazanowska
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Banasik
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Marcelina
authors.initials: M
authors.lastname: Żabinska
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Boratyńska
authors.affiliation: Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Lepiesza
authors.affiliation: Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Korta
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, Chałubińskiego 6a, 50-368 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gomółkiewicz
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, Chałubińskiego 6a, 50-368 Wrocław, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dzięgiel
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Klinger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016207:Cytokines / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D010530:Peritoneal Dialysis; D006435:Renal Dialysis
chemical_list: D016207:Cytokines