title: Relationship between pathological findings and enzymes of the energy metabolism in liver of rats infected by Trypanosoma evansi.
abstract: The aim of this study was to investigate the activities of important enzymes involved in the energetic metabolism in the liver of rats experimentally infected by Trypanosoma evansi. Adenylate kinase (AK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver homogenate, as well as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and clotting time in plasma were evaluated at 5 and 15 days post-infection (PI). The activities of the respiratory chain complexes and of Na(+), K(+)-ATPase were also evaluated. This study demonstrates energetic metabolism impairment in rats infected by T. evansi. A reduced energy metabolism in the liver of rats infected by T. evansi was observed, demonstrated by AK decreased and PK increased activities at 5 days PI, a mechanism known as energetic compensation. However, at 15 days PI a decrease of AK and PK activities were observed. In addition, an increase in the activities of respiratory chain complexes II, II-III and IV in infected rats at 15 days PI, and a decrease of Na(+), K(+)-ATPase activities in infected rats on days 5 and 15 PI were verified. In the plasma, we observed an increase in ALT and AST activities on days 5 and 15 PI, and increase in clotting time in infected rats. The changes caused by T. evansi infection on the activity of enzymes of hepatic energy metabolism can corroborate to elucidate the mechanisms that lead to liver injury and inflammatory infiltration verified in T. evansi infected rats. Therefore, these alterations are directly related to disease pathogenesis.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: matheusd.biomed@yahoo.com.br.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Rech
authors.affiliation: Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Mateus
authors.initials: M
authors.lastname: Grings
authors.affiliation: Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Janaína
authors.initials: J
authors.lastname: Kolling
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Lucas T
authors.initials: LT
authors.lastname: Gressler
authors.affiliation: Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Carine De F
authors.initials: Cde F
authors.lastname: Souza
authors.affiliation: Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Vaucher
authors.affiliation: Section of Veterinary Pathology, Instituto Federal Catarinense, Concórdia, SC, Brazil.
authors.forename: Claiton I
authors.initials: CI
authors.lastname: Schwertz
authors.affiliation: Section of Veterinary Pathology, Instituto Federal Catarinense, Concórdia, SC, Brazil.
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Mendes
authors.affiliation: Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Guilhian
authors.initials: G
authors.lastname: Leipnitz
authors.affiliation: Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Angela T S
authors.initials: AT
authors.lastname: Wyse
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Lenita M
authors.initials: LM
authors.lastname: Stefani
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: sgmonteiro@uol.com.br.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism*; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000469:parasitology; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014345:Trypanosoma / Q000472:pathogenicity; D014352:Trypanosomiasis / Q000469:parasitology / Q000473:pathology*; D014914:Whole Blood Coagulation Time
chemical_list: D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D011770:Pyruvate Kinase; D000263:Adenylate Kinase; D000254:Sodium-Potassium-Exchanging ATPase
keywords: ATPase; Adenylate kinase; Hepatic tissue; Pyruvate kinase; Trypanosomosis