title: CD3+ICOS+T cells show differences in the synthesis of nitric oxide, IFN-γ, and IL-10 in patients with pulmonary tuberculosis or in healthy household contacts.
abstract: Evidence indicates that more than 90 % of infected individuals never develop active tuberculosis. This fact highlights the relevance of the immune response in tuberculosis control. The inducible co-stimulator (ICOS) is a regulator of the function, differentiation, proliferation, and activation of T cells. Moreover, T cells synthesise nitric oxide (NO), interferon gamma (IFN-γ), and interleukin (IL)-10, which help regulate the immune response to tuberculosis. Therefore, we assessed the synthesis of NO, IFN-γ, and IL-10 in CD3+ICOS+T cells from healthy individuals, household contacts (HHC), and patients with active pulmonary tuberculosis (PTB), previously stimulated with the antigen H37Rv. Our results indicated a significant increase in both the percentage of ICOS+cells and CD3+ICOS+T cells producing NO, IFN-γ, and IL-10 in cells obtained from patients with PTB (p < 0.01). In addition, a high mitochondrial membrane potential (ΔΨm) in CD3+ICOS+T cells was observed in the cells from HHC and from PTB patients, and is associated with the activation of T cells. In conclusion, results show that the CD3+ICOS+T cells obtained from PTB patients are the main producers of NO, IFN-γ, and IL-10. In addition, our results imply that NO is a modulator of ICOS expression of T cells from PTB patients.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, UNAM, Mexico D.F., Mexico. mlara@ujsierra.com.mx.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lara-Rodríguez
authors.affiliation: Department of Biochemistry, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico D.F., Mexico.
authors.forename: Noé
authors.initials: N
authors.lastname: Alvarado-Vásquez
authors.affiliation: Department of Biochemistry, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico D.F., Mexico.
authors.forename: Demetrio
authors.initials: D
authors.lastname: Bernal
authors.affiliation: Department of Biochemistry, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico D.F., Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gorocica
authors.affiliation: Department of Biochemistry, Faculty of Medicine, UNAM, Mexico D.F., Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.affiliation: Department of Biochemistry, Faculty of Medicine, UNAM, Mexico D.F., Mexico.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lascuraín
mesh_terms: D000328:Adult; D017252:CD3 Complex / Q000378:metabolism; D002478:Cells, Cultured; D005190:Family; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D006867:Hydrolases / Q000276:immunology; D060889:Inducible T-Cell Co-Stimulator Protein / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000378:metabolism
chemical_list: D017252:CD3 Complex; C556692:ICOS protein, human; C573415:IFNG protein, human; C508609:IL10 protein, human; D060889:Inducible T-Cell Co-Stimulator Protein; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma; D006867:Hydrolases; C527647:HsaD protein, Mycobacterium tuberculosis
keywords: Healthy house contacts; Inducible co-stimulator molecule; Interleukins; Mitochondria; Nitric oxide; Pulmonary tuberculosis; T cells