title: Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization.
abstract: BACKGROUND
  Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel.
  
  
  METHODS
  The identification took place based on the morphometrics of the spicules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each animal) at the slaughterhouses from different localities in Iran. Samples were morphologically identified according to the spicules' morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.
  
  
  RESULTS
  PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. contortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different fragments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.
  
  
  CONCLUSIONS
  The genotypic results are in agreement with the phenotypic findings of both species.
authors.affiliation: Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).
authors.forename: Behnam
authors.initials: B
authors.lastname: Meshgi
authors.affiliation: Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Jalousian
authors.affiliation: Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).
authors.forename: Zahra
authors.initials: Z
authors.lastname: Masih
keywords: Haemonchus species; ITS2; Morphology; PCR-RFLP