title: Direct determination of fatty acid esters of 3-chloro-1, 2-propanediol in edible vegetable oils by isotope dilution - ultra high performance liquid chromatography - triple quadrupole mass spectrometry.
abstract: A selective and sensitive ultra-high performance liquid chromatography - triple quadrupole mass spectrometry (UHPLC-MS/MS) method coupled with matrix solid phase dispersion (MSPD) extraction was developed for the direct determination of fatty acid esters of 3-chloro-1,2-propanediol (3-MCPD esters) in edible vegetable oils. The method integrated the isotope dilution technique, MSPD extraction and UHPLC - MS/MS analysis with multi-reaction monitoring mode (MRM). Matrix-matched calibration curves showed good linearity within the range of 0.01-10mgL(-1) with the correlation coefficient not less than 0.999. Limits of detection (LODs) and limit of quantification (LOQs) of the 3-MCPD esters fell into the range of 0.0001-0.02mgkg(-1) and 0.0004-0.05mgkg(-1), respectively. The recoveries for the spiked extra virgin olive oils ranged from 94.4% to 108.3%, with the relative standard deviations (RSD) ranging from 0.6% to 10.5%. The method was applied for the oil sample (T2642) of the official Food Analysis Performance Assessment Scheme (FAPAS) in 2014 and other real samples from supermarket, and the results showed that the present method was comparative to the gas chromatography-mass spectrometry (GC-MS) method based on the improved German Society for Fat Science (DGF) standard method C-III 18 (09) except for palm oil.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Food Safety Risk Assessment, Ministry of Health; China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Heli
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health; China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health; China National Center for Food Safety Risk Assessment, Beijing 100021, China. Electronic address: miaoh@cfsa.net.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Miao
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health; China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Food Safety Risk Assessment, Ministry of Health; China National Center for Food Safety Risk Assessment, Beijing 100021, China. Electronic address: wuyongning@cfsa.net.cn.
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis; D007201:Indicator Dilution Techniques; D007554:Isotopes; D057230:Limit of Detection; D010938:Plant Oils / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods; D000517:alpha-Chlorohydrin / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D007554:Isotopes; D010938:Plant Oils; D000517:alpha-Chlorohydrin
keywords: 2-propanediol (3-MCPD esters); Edible vegetable oils; Fatty acid esters of 3-chloro-1; Isotope dilution; Matrix solid phase dispersion (MSPD); Ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS)