title: [Interfering effect of an intergenic-derived sRNA of Cryptococcus neoformans].
abstract: OBJECTIVE
  We demonstrated the interfering effect of a new intergenic-derived sRNA of Cryptococcus neoformans.
  
  
  METHODS
  We constructed a library of sRNAs from 20 to 25 nt, and used a reporter gene CLC1 that was fused to the testing sRNAs to show the interference effect, i. e. the degradation of the CLC1 would gene rate an albino phenotype of the transformants.
  
  
  RESULTS
  Through the CLC-sRNA reporter system, we acquired one sRNA, sRNA-1, located to an intergenic region of the genome of C. neoformans, that displayed interfering effect on CLCl.
  
  
  CONCLUSIONS
  In C. neoformans, the origination of sRNA may be diverse. The endogenous sRNA should play interfering function via the canonical RNAi pathway.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Sun
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D003455:Cryptococcus neoformans / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012331:RNA, Fungal / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D034741:RNA, Small Interfering