title: Interaction between TBP and Condensin Drives the Organization and Faithful Segregation of Mitotic Chromosomes.
abstract: Genome/chromosome organization is highly ordered and controls various nuclear events, although the molecular mechanisms underlying the functional organization remain largely unknown. Here, we show that the TATA box-binding protein (TBP) interacts with the Cnd2 kleisin subunit of condensin to mediate interphase and mitotic chromosomal organization in fission yeast. TBP recruits condensin onto RNA polymerase III-transcribed (Pol III) genes and highly transcribed Pol II genes; condensin in turn associates these genes with centromeres. Inhibition of the Cnd2-TBP interaction disrupts condensin localization across the genome and the proper assembly of mitotic chromosomes, leading to severe defects in chromosome segregation and eventually causing cellular lethality. We propose that the Cnd2-TBP interaction coordinates transcription with chromosomal architecture by linking dispersed gene loci with centromeres. This chromosome arrangement can contribute to the efficient transmission of physical force at the kinetochore to chromosomal arms, thereby supporting the fidelity of chromosome segregation.
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Osamu
authors.initials: O
authors.lastname: Iwasaki
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanizawa
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Kyoung-Dong
authors.initials: KD
authors.lastname: Kim
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Yokoyama
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Corcoran
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Atsunari
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Skordalakes
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA.
authors.forename: Louise C
authors.initials: LC
authors.lastname: Showe
authors.affiliation: The Wistar Institute, Philadelphia, PA19104, USA. Electronic address: noma@wistar.org.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Noma
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D008938:Mitosis; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D054730:Protein Interaction Domains and Motifs; D021122:Protein Subunits; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C454839:Cnd2 protein, S pombe; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; D029702:Schizosaccharomyces pombe Proteins; D035181:TATA-Box Binding Protein; C108312:condensin complexes; D012320:RNA Polymerase III; D000251:Adenosine Triphosphatases