title: Mouse Polyomavirus: Propagation, Purification, Quantification, and Storage.
abstract: Mouse polyomavirus (MPyV) is a member of the Polyomaviridae family, which comprises non-enveloped tumorigenic viruses infecting various vertebrates including humans and causing different pathogenic responses in the infected organisms. Despite the variations in host tropism and pathogenicity, the structure of the virions of these viruses is similar. The capsid, with icosahedral symmetry (ø, 45 nm, T = 7d), is composed of a shell of 72 capsomeres of structural proteins, arranged around the nucleocore containing approximately 5-kbp-long circular dsDNA in complex with cellular histones. MPyV has been one of the most studied polyomaviruses and serves as a model virus for studies of the mechanisms of cell transformation and virus trafficking, and for use in nanotechnology. It can be propagated in primary mouse cells (e.g., in whole mouse embryo cells) or in mouse epithelial or fibroblast cell lines. In this unit, propagation, purification, quantification, and storage of MPyV virions are presented.
authors.affiliation: Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Horníková
authors.affiliation: Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic.
authors.forename: Vojtěch
authors.initials: V
authors.lastname: Žíla
authors.affiliation: Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Španielová
authors.affiliation: Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic. jitkaf@natur.cuni.cz.
authors.forename: Jitka
authors.initials: J
authors.lastname: Forstová
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D051379:Mice; D011120:Polyomavirus / Q000254:growth & development* / Q000302:isolation & purification*; D011309:Preservation, Biological / Q000379:methods*; D019562:Viral Load / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*
keywords: density gradient purification; mouse polyomavirus; polyomavirus; virus quantification