title: Genetic characterization of porcine kobuvirus variants identified from healthy piglets in China.
abstract: In this study, two porcine kobuvirus strains, JS-01-CHN and JS-02a-CHN were detected from piglets with diarrhea and asymptomatic, respectively. The sequences of the two strains were analyzed using a bioinformatics software package. The full-length genome of JS-02a-CHN, was detected in healthy piglets was 8121 nucleotides (nt) long excluding the poly(A) tail. There was a 30 amino acid deletion in the 2B-coding region of JS-02a-CHN. We are the first to report a 30 amino acid deletion in porcine kobuvirus from asymptomatic piglets, indicating that porcine kobuvirus may have evolved differently based on geography and host differences. Fecal samples were obtained from pigs with diarrhea (n=91) and healthy (n=126) pigs and analyzed using RT-PCR. Of these, 64.8% (59/91) of diarrheic piglets and 19.8% (25/126) of healthy piglets were positive for PKV using VP1 specific primers. Twenty-eight (28) virus positive samples were randomly selected and the VP1 gene was analyzed. Phylogenetic analysis indicated that the 15 strains isolated from pigs with diarrhea clustered into different branches, while the VP1 sequences from clinically healthy pigs clustered into a single large group. These results indicate that the VP1 gene is diverse in pigs with diarrhea but conserved in healthy pigs in the Jiangsu Province.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China; College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: wenjiejin1@163.com.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Jin
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
authors.forename: Zhen-Peng
authors.initials: ZP
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: aijian@yzu.edu.cn.
authors.forename: Ai-Jian
authors.initials: AJ
authors.lastname: Qin
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China. Electronic address: yanghanchun1@cau.edu.cn.
authors.forename: Han-Chun
authors.initials: HC
authors.lastname: Yang
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002681:China; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D052699:Kobuvirus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D058974:Phylogeography; D010850:Picornaviridae Infections / Q000662:veterinary / Q000821:virology; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology
chemical_list: D036022:Capsid Proteins
keywords: Bioinformatics; Complete genome sequence; Porcine kobuvirus; VP1 gene