title: Visualizing fluoride ion in mitochondria and lysosome of living cells and in living mice with positively charged ratiometric probes.
abstract: Two ratiometric probes fluoride ion, Mito-F and Lyso-F, were rationally designed and synthesized with positive charges at physiological conditions. The positive charges functioned as target moieties for subcellular mitochondria and lysosome of living cells, and effective sequesters of fluoride ion for fast and efficient fluorescent detection. In addition, in vivo imaging of fluoride ion in living mice was successfully achieved for the first time using probe Mito-F.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, The School of Pharmaceutical Sciences, Peking University , Beijing 100191, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, The School of Pharmaceutical Sciences, Peking University , Beijing 100191, China.
authors.forename: Xinjing
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D005459:Fluorides / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D005456:Fluorescent Dyes; D005459:Fluorides