title: Solution properties of a heteropolysaccharide extracted from pumpkin (Cucurbita pepo, lady godiva).
abstract: A water-soluble galactoglucofucomannan was extracted from pumpkin (Cucurbita pepo, lady godiva variety). GC-MS analysis indicated that the polysaccharide was composed of 1,6-linked-glucosyl, 1,2,6-linked-mannosyl, 1,3,6-linked-mannosyl, 1,2,6-linked-galactosyl, 1,2,6-linked-galactosyl, terminal fucosyl and terminal glucose. The solution properties of the polysaccharide were studied systematically by using size-exclusion chromatography combined with multi-angle laser light scattering, viscometry and dynamic light scattering at 25 °C. The weight average molecular masses (Mw), intrinsic viscosity [η], radius of gyration (Rg) and hydrodynamic radius (Rh) were found to be 12.7 × 10(5)g/mol, 780 ml/g, 68 nm and 116 nm, respectively. The fraction dimension and value of ρ (Rg/Rh) of the polysaccharide revealed that it existed in a sphere-like conformation in distilled water. The dependence of zero shear specific viscosity on the coil overlap parameter was analyzed using different models. Furthermore, degradation of samples upon autoclaving has been observed and quantified by intrinsic viscosity determination and SEC-MALLS.
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Ni
authors.affiliation: National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: sy587@hotmail.com.
authors.forename: Quanhong
authors.initials: Q
authors.lastname: Li
mesh_terms: D028464:Cucurbita / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008027:Light; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008745:Methylation; D012542:Scattering, Radiation; D012996:Solutions / Q000737:chemistry; D014783:Viscosity
chemical_list: D008351:Mannans; D012996:Solutions; C443804:galactoglucomannan
keywords: Polysaccharide; Pumpkin; Solution property