title: Prevalence of HLA-B27 in Moroccan healthy subjects and patients with ankylosing spondylitis and mapping construction of several factors influencing AS diagnosis by using multiple correspondence analysis.
abstract: The aim of the present study was to determine the prevalence of human leukocyte antigen HLA-B27 in Moroccan healthy controls and in patients with ankylosing spondylitis (AS), and to analyze the correlation between HLA-B27 and AS in Moroccan patients. The prevalence of HLA-B27 was determined by evaluating the number of HLA-B27-positive samples in 128 healthy subjects and in 53 patients diagnosed with AS according to the ESSG and AMOR criteria. HLA-B27 was determined by the polymerase chain reaction using sequence-specific primers. Multivariate analysis of our data (HLA-B27, age, sex, and family history) for AS and healthy controls was performed by multiple correspondence analysis (MCA). The frequency of HLA-B27 was significantly greater in AS patients (45.3 %) than in healthy controls (4.7 %) [p < 0.0001, OR 16.8, and CI 95 % (5.83-51.03)]. In addition, HLA-B27 was more common in male patients than in female ones (p < 0.05). 100 % of the AS patients reported a family history of AS, whereas only 20 % of the healthy controls reported a family history of AS. The graphical interpretation of MCA showed a significant relation between the presence of HLA-B27 and AS. This study strengthens the link between HLA-B27 and AS and represents a very valuable informative diagnostic tool, especially in regard to male patients who have a family history of AS.
authors.affiliation: Laboratory of Biochemistry and Immunology, Faculty of Sciences, University Mohammed V-Agdal, Avenue Ibn Battouta, BP1014, Rabat, Morocco. akassouamal@gmail.com.
authors.forename: Amal
authors.initials: A
authors.lastname: Akassou
authors.affiliation: Rheumatology Department, Mohammed VI Medical Center, Marrakesh, Morocco.
authors.forename: Hanae
authors.initials: H
authors.lastname: Yacoubi
authors.affiliation: Laboratory of Biochemistry and Immunology, Faculty of Sciences, University Mohammed V-Agdal, Avenue Ibn Battouta, BP1014, Rabat, Morocco.
authors.forename: Afaf
authors.initials: A
authors.lastname: Jamil
authors.affiliation: Laboratory of Biochemistry and Immunology, Faculty of Sciences, University Mohammed V-Agdal, Avenue Ibn Battouta, BP1014, Rabat, Morocco.
authors.forename: Nadia
authors.initials: N
authors.lastname: Dakka
authors.affiliation: Laboratory of Biochemistry and Immunology, Faculty of Sciences, University Mohammed V-Agdal, Avenue Ibn Battouta, BP1014, Rabat, Morocco.
authors.forename: Saaïd
authors.initials: S
authors.lastname: Amzazi
authors.affiliation: Laboratory of Biochemistry and Immunology, Faculty of Sciences, University Mohammed V-Agdal, Avenue Ibn Battouta, BP1014, Rabat, Morocco.
authors.forename: Khalid
authors.initials: K
authors.lastname: Sadki
authors.affiliation: Rheumatology Department, Mohammed VI Medical Center, Marrakesh, Morocco.
authors.forename: Redouane
authors.initials: R
authors.lastname: Niamane
authors.affiliation: Rheumatology Department, Mohammed VI Medical Center, Marrakesh, Morocco.
authors.forename: Selma
authors.initials: S
authors.lastname: Elhassani
authors.affiliation: Laboratory of Biochemistry and Immunology, Faculty of Sciences, University Mohammed V-Agdal, Avenue Ibn Battouta, BP1014, Rabat, Morocco.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Bakri
mesh_terms: D000293:Adolescent; D000328:Adult; D044383:Black People / Q000235:genetics; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D015796:HLA-B27 Antigen / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009018:Morocco / Q000453:epidemiology; D015999:Multivariate Analysis; D016017:Odds Ratio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors; D017678:Sex Distribution; D012737:Sex Factors; D013167:Spondylitis, Ankylosing / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D015796:HLA-B27 Antigen
keywords: Ankylosing spondylitis; HLA-B27; Moroccan population; Multiple correspondence analysis