title: Streptococcus mutans copes with heat stress by multiple transcriptional regulons modulating virulence and energy metabolism.
abstract: Dental caries is closely associated with the virulence of Streptococcus mutans. The virulence expression of S. mutans is linked to its stress adaptation to the changes in the oral environment. In this work we used whole-genome microarrays to profile the dynamic transcriptomic responses of S. mutans during physiological heat stress. In addition, we evaluated the phenotypic changes, including, eDNA release, initial biofilm formation, extracellular polysaccharides generation, acid production/acid tolerance, and ATP turnover of S. mutans during heat stress. There were distinct patterns observed in the way that S. mutans responded to heat stress that included 66 transcription factors for the expression of functional genes being differentially expressed. Especially, response regulators of two component systems (TCSs), the repressors of heat shock proteins and regulators involved in sugar transporting and metabolism co-ordinated to enhance the cell's survival and energy generation against heat stress in S. mutans.
authors.affiliation: 1] State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [2] Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [3] Center for Oral Health and Systemic Disease, School of Dentistry, University of Louisville, Louisville, Kentucky, USA.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Lab of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, PR China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Niu
authors.affiliation: 1] State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [2] Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 1] State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [2] Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Shida
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Mingyun
authors.initials: M
authors.lastname: Li
authors.affiliation: 1] State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [2] Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Jiyao
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Lab of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: 1] State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [2] Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Center for Oral Health and Systemic Disease, School of Dentistry, University of Louisville, Louisville, Kentucky, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Lamont
authors.affiliation: 1] State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China [2] Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
mesh_terms: D018441:Biofilms / Q000254:growth & development; D003731:Dental Caries / Q000382:microbiology; D004734:Energy Metabolism / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D006358:Hot Temperature; D018085:Regulon / Q000235:genetics*; D013295:Streptococcus mutans / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014774:Virulence / Q000235:genetics*
chemical_list: D014157:Transcription Factors