title: Accumulation and effects of sediment-associated silver nanoparticles to sediment-dwelling invertebrates.
abstract: Sediment is increasingly recognized as the major sink for contaminants including nanoparticles (NPs). Thus, sediment-living organisms are especially susceptible to NP exposure. Studies of the fate and effects of NPs in the sediment matrix are still in their infancy, and data from such studies are in high demand. Here, we examine the effects of exposure to sediment mixed with either aqueous Ag (administered as AgNO3) or Ag NPs (13nm, citrate-capped) at a nominal exposure concentration of 100μg Ag/g dry weight sediment on four benthic invertebrates: two clones of the gastropod Potamopyrgus antipodarum (clones A and B) and two polychaete species (Capitella teleta, Capitella sp. S). Our results show that both species sensitivity and Ag form (aqueous Ag, Ag NPs) play a role in bioaccumulation and effects. Following two weeks of exposure, both clones of P. antipodarum were found to be insensitive towards both Ag forms (generally low Ag accumulation, no toxicity). In contrast, the two Capitella species varied widely with respect to Ag uptake and observed toxicity. Capitella sp. S was adversely affected by both aqueous Ag (mortality) and Ag NPs (growth), whereas C. teleta was not affected by either Ag form. For neither polychaete species was the observed toxicity directly related to bioaccumulation. Therefore, future nano-ecotoxicological research should focus on understanding differences in uptake and handling mechanisms among species and the relationship between bioaccumulation and toxicity.
authors.affiliation: Department of Environmental, Social and Spatial Change, Roskilde University, Universitetsvej 1, DK-4000 Roskilde, Denmark. Electronic address: tramskov@hotmail.com.
authors.forename: Tina
authors.initials: T
authors.lastname: Ramskov
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, 348 Manter Hall, Lincoln 68588, USA. Electronic address: vforbes3@unl.edu.
authors.forename: Valery E
authors.initials: VE
authors.lastname: Forbes
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (VA), Italy. Electronic address: douglas.gilliland@jrc.ec.europa.eu.
authors.forename: Douglas
authors.initials: D
authors.lastname: Gilliland
authors.affiliation: Department of Environmental, Social and Spatial Change, Roskilde University, Universitetsvej 1, DK-4000 Roskilde, Denmark. Electronic address: selck@ruc.dk.
authors.forename: Henriette
authors.initials: H
authors.lastname: Selck
mesh_terms: D000818:Animals; D049851:Gastropoda / Q000187:drug effects* / Q000378:metabolism; D019015:Geologic Sediments / Q000737:chemistry*; D053758:Nanoparticles / Q000378:metabolism / Q000633:toxicity*; D011077:Polychaeta / Q000187:drug effects* / Q000378:metabolism; D012834:Silver / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D012834:Silver
keywords: Biokinetics; Deposit-feeders; Ecotoxicity; Invertebrates; Sediment exposure; Silver nanoparticles