title: Cell outer membrane mimetic chitosan nanoparticles: preparation, characterization and cytotoxicity.
abstract: A negatively charged copolymer poly (MPC-co-AMPS) of 2-methacryloyloxyethyl phosphorylcholine (MPC) and 2-acrylamide-2-methyl propane sulfonic acid (AMPS) was designed and synthesized. Chitosan nanoparticles with cell outer membrane mimetic structure were prepared by electrostatic interaction between the sulfonic acid groups of poly (MPC-co-AMPS) and the protonated amino groups of chitosan. Effects of factors on influencing the particle size, distribution, and stability were investigated. The experimental results showed that cell membrane mimetic chitosan nanoparticles with controllable and homogeneous size ranged from 100 to 300 nm were prepared at the concentration of 0.1-2.0 mg/mL and the charge ratio of 0.5-1.1. Chitosan nanoparticles prepared can exist stably for more than 45 days when placed at 4 °C and pH < 7.5. The cytotoxicity of the chitosan nanoparticles reduced significantly after surface modification with cell membrane mimetic structure, meeting the basic requirements of biomedical materials. The results suggest cell membrane mimetic chitosan nanoparticles prepared with polyanion and polycation obtain good biological compatibility and immune stealth ability, which has important academic significance and great application prospects.
authors.affiliation: a College of Environmental and Chemical Engineering, Xi'an Polytechnic University , Xi'an 710048 , Shaanxi , P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Liang
authors.forename: Lingheng
authors.initials: L
authors.lastname: Kong
authors.forename: Lina
authors.initials: L
authors.lastname: Zheng
authors.forename: Tao
authors.initials: T
authors.lastname: Fan
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D002462:Cell Membrane; D002470:Cell Survival; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity; D010316:Particle Size; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D012972:Sodium Hydroxide / Q000737:chemistry; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
chemical_list: D011109:Polymethacrylic Acids; D012996:Solutions; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D012972:Sodium Hydroxide; D048271:Chitosan
keywords: characterization; chitosan; nanoparticles; phosphorylcholine; preparation