title: Polymorphobacter fuscussp. nov., isolated from permafrost soil, and emended description of the genusPolymorphobacter.
abstract: Strain D40PT, representing a novel Gram-stain-negative, obligately aerobic, bacteriochlorophylla-containing bacterium of the α-4 subgroup of the phylumProteobacteria, was isolated from permafrost soil of Kunlun mountains gap, Qinghai-Tibet plateau. Cells were non-motile rod-cocci and formed brown-pigmented colonies. According to the absorption spectrum, carotenoids and two different photosynthetic light-harvesting complexes, an LHI complex and a B800-835-type peripheral LHII complex, were present in the cells. The strain was oxidase-negative and catalase-positive. The predominant fatty acids of strain D40PTwere summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c), C17 : 1ω6cand summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c). The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid, two glycolipids and sphingoglycolipid. The major respiratory quinone was ubiquinone-10, whereas ubiquinone-9 was present in smaller amounts. The 16S rRNA gene sequence similarity to the closest phylogenetic relative,Polymorphobacter multimaniferJCM 18140T, was 97.5 %. DNA-DNA relatedness (ΔTm) between strain D40PTandP. multimaniferwas 12.4 °C. The G+C content of the genomic DNA of strain D40PTwas 67.4 mol%. Accordingly, the strain represents a novel species, for which the namePolymorphobacter fuscussp. nov. is proposed. The type strain is D40PT( = CGMCC 1.12714T= JCM 19740T). An emended description of the genusPolymorphobacteris also proposed.
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.|College of Resources and Environmental Science, East China Normal University, Shanghai 200241, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Hou
authors.affiliation: Oil and Gas Survey, China Geological Survey, Beijing 100029, PR China.
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Lu
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lv