title: Surface Chemical Gradient Affects the Differentiation of Human Adipose-Derived Stem Cells via ERK1/2 Signaling Pathway.
abstract: To understand the role of surface chemistry on cell behavior and the associated molecular mechanisms, we developed and utilized a surface chemical gradient of amine functional groups by carefully adjusting the gas composition of 1,7-octadiene (OD) and allylamine (AA) of the plasma phase above a moving substrate. The chemical gradient surface used in the present work shows an increasing N/C ratio and wettability from the OD side toward the AA side with no change in surface topography. Under standard culture conditions (with serum), human adipose-derived stem cells (hASCs) adhesion and spreading area increased toward the AA side of the gradient. However, there were no differences in cell behavior in the absence of serum. These results, supported by the trends in proteins adsorption on the gradient surface, demonstrated that surface chemistry affects the response of hASCs through cell-adhesive serum proteins, rather than interacting directly with the cells. The expression of p-ERK and the osteogenic differentiation increased toward the AA side of the gradient, while adipogenic differentiation decreased in the same direction; however, when the activation of ERK1/2 was blocked by PD98059, the levels of osteogenic or adipogenic differentiation on different regions of the chemical gradient were the same. This indicates that ERK1/2 may be an important downstream signaling pathway of surface chemistry directed stem cell fate.
authors.affiliation: Graduate School at Shenzhen, Tsinghua University , Shenzhen 518055, China.|State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Xujie
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Burns Department of Zhujiang Hospital, Southern Medical University , Guangzhou 510280, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Shi
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.|Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Mawson Institute, University of South Australia , Mawson Lakes 5095, Australia.
authors.forename: Akash
authors.initials: A
authors.lastname: Bachhuka
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Mawson Institute, University of South Australia , Mawson Lakes 5095, Australia.|School of Advanced Manufacturing, University of South Australia , Mawson Lakes 5095, Australia.
authors.forename: Krasimir
authors.initials: K
authors.lastname: Vasilev
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000475:Alkenes / Q000737:chemistry / Q000494:pharmacology; D000499:Allylamine / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D000475:Alkenes; D009930:Organic Chemicals; C466090:PD 98058; D000499:Allylamine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C546881:1,7-octadiene
keywords: ERK1/2 activation; adipose-derived stem cell; differentiation; plasma polymerization; surface chemical gradient