title: Dietary supplementation with L-glutamate and L-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide.
abstract: This study was to evaluate the protective roles of L-glutamate (Glu) and L-aspartate (Asp) in weaned piglets challenged with H2O2. Forty weaned piglets were assigned randomly into one of five groups (8 piglets/group): (1) control group (NC) in which pigs were fed a corn- and soybean meal-based diet and received intraperitoneal administration of saline; (2) H2O2 group (PC) in which pigs were fed the basal diet and received intraperitoneal administration of 10 % H2O2 (1 ml/kg body weight once on days 8 and repeated on day 11); (3) PC + Glu group (PG) in which pigs were fed the basal diet supplemented with 2.0 % Glu before intraperitoneal administration of 10 % H2O2; (4) PC + Asp group (PA) in which pigs were fed the basal diet supplemented with 1.0 % Asp before intraperitoneal administration of 10 % H2O2; (5) PC + Glu + Asp group (PGA) in which pigs were fed the basal diet supplemented with 2.0 % Glu plus 1.0 % Asp before intraperitoneal administration of 10 % H2O2. Measured parameters included daily feed intake (DFI), average daily gain (ADG), feed conversion rate (FCR), and serum anti-oxidative enzyme activities (catalase, superoxide dismutase, glutathione peroxidase-1), serum malondialdehyde and H2O2 concentrations, serum amino acid (AA) profiles, and intestinal expression of AA transporters. Dietary supplementation with Glu, Asp or their combination attenuated the decreases in DFI, ADG and feed efficiency, the increase in oxidative stress, the alterations of serum AA concentrations, and the changed expression of intestinal AA transporters in H2O2-challenged piglets. Thus, dietary supplementation with Glu or Asp alleviates growth suppression and oxidative stress, while restoring serum the amino acid pool in H2O2-challenged piglets.
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Jielin
authors.initials: J
authors.lastname: Duan
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Cui
authors.affiliation: College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Huang
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Animal Science, North Carolina State University, Raleigh, 27695, USA.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China. Tjli@isa.ac.cn.|Changsha Lvye Biotechnology Limited Company Academician Expert Workstation, Changsha, 410128, Hunan, China. Tjli@isa.ac.cn.|Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangzhou, 510663, Guangdong, China. Tjli@isa.ac.cn.|Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additives and Animal Intestinal Health, Guangzhou, 511400, Guangdong, China. Tjli@isa.ac.cn.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China. yinyulong@isa.ac.cn.|School of Biology, Hunan Normal Univesity, Changsha, 410018, Hunan, China. yinyulong@isa.ac.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D019587:Dietary Supplements / Q000032:analysis*; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000009:adverse effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D013552:Swine / Q000254:growth & development / Q000378:metabolism*; D014886:Weaning
chemical_list: D001224:Aspartic Acid; D018698:Glutamic Acid; D006861:Hydrogen Peroxide
keywords: Hydrogen peroxide; L-Aspartate; L-Glutamate; Oxidative stress; Performance; Pigs