title: 'One-component' ultrathin multilayer films based on poly(vinyl alcohol) as stabilizing coating for phenytoin-loaded liposomes.
abstract: Ultrathin "one-component" multilayer polymeric films for potential biomedical applications were designed based on polyvinyl alcohol,-a non-toxic, fully degradable synthetic polymer. Good uniformity of the obtained film and adequate adsorption properties of the polymeric layers were achieved by functional modification of the polymer, which involved synthesis of cationic and anionic derivatives. Synthesized polymers were characterized by FTIR, NMR spectroscopy, dynamic light scattering measurements and elemental analysis. The layer by layer assembly technique was used to build up a multilayer film and this process was followed using UV-Vis spectroscopy and ellipsometry. The morphology and thickness of the obtained multilayered film material was evaluated by atomic force microscopy (AFM). Preliminary studies on the application of the obtained multilayer film for coating of liposomal nanocarriers containing phenytoin, an antiarrhythmic drug, were performed. The coating effectively stabilizes liposomes and the effect increases with an increasing number of deposited layers until the polymeric film reaches the optimal thickness. The obtained release profiles suggest that bilayer-coated liposomes release phenytoin less rapidly than uncoated ones. The cytotoxicity studies performed for all obtained nanocarriers confirmed that none of them has negative effect on cell viability. All of the performed experiments suggest that liposomes coated with ultrathin film obtained from PVA derivatives can be attractive drug nanocarriers.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zasada
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Łukasiewicz-Atanasov
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kłysik
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Lewandowska-Łańcucka
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gzyl-Malcher
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Puciul-Malinowska
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland. Electronic address: karewicz@chemia.uj.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Karewicz
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30- 060 Kraków, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
mesh_terms: D000328:Adult; D000889:Anti-Arrhythmia Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008081:Liposomes; D018625:Microscopy, Atomic Force; D010672:Phenytoin / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012995:Solubility
chemical_list: D000889:Anti-Arrhythmia Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008081:Liposomes; D010672:Phenytoin; D011142:Polyvinyl Alcohol
keywords: Coating; Liposome; Multilayer film; Phenytoin; Poly(vinyl alcohol) derivative