title: Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins.
abstract: We showed that bee venom phospholipase A2 can be used as a membrane-binding vector to anchor to the surface of cells a soluble protein fused to its C-terminus. ZZ, a two-domain derivative of staphylococcal protein A capable of binding constant regions of antibodies was fused to the C-terminus of the phospholipase or to a mutant devoid of enzymatic activity. The fusion proteins bound to the surface of cells and could themselves bind IgGs. Their fate depended on the cell type to which they bound. On the A431 carcinoma cell line the proteins remained exposed on the cell surface. In contrast, on human dendritic cells the proteins were internalized into early endosomes.
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Babon
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Wurceldorf
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Almunia
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Pichard
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Chenal
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Buhot
authors.affiliation: FRE 3689 CNRS-UM, 1919 Route de Mende, 34293 Montpellier Cedex 05, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Beaumelle
authors.affiliation: CEA, iBiTecS, SIMOPRO, LabEx LERMIT, Gif sur Yvette F-91191, France. Electronic address: daniel.gillet@cea.fr.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gillet
mesh_terms: D000818:Animals; D001514:Bee Venoms / Q000201:enzymology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism; D006801:Humans; D054467:Phospholipases A2 / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry
chemical_list: D001514:Bee Venoms; D011993:Recombinant Fusion Proteins; D054467:Phospholipases A2
keywords: Bee venom phospholipase A(2); Dendritic cells; Membrane anchor; Staphylococcal protein A