title: Identification and biochemical characterization of a novel endo-type β-agarase AgaW from Cohnella sp. strain LGH.
abstract: An agar-degrading bacterium, strain LGH, was isolated and identified as Cohnella sp. This strain had a capability of utilizing agar as a sole carbon source for growth and showed a strong agarolytic activity. A novel endo-type β-agarase gene agaW, encoding a primary translation product of 891 amino acids, including a 26 amino acid signal peptide, was cloned and identified from a genomic library of strain LGH. The AgaW belonged to the glycoside hydrolase (GH) GH50 family, with less than 39% amino acid sequence similarity with any known protein, and hydrolyzed agarose into neoagarotetraose as the major end product and neoagarobiose as the minor end product through other neoagarooligosaccharide intermediates, such as neoagarohexaose.
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Gen
authors.initials: G
authors.lastname: Li
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China. wujun2013@njau.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, People's Republic of China.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Ge
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Li
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hu
mesh_terms: D056449:Bacillales / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002244:Carbon / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004187:Disaccharides / Q000378:metabolism; D005697:Galactosides / Q000378:metabolism; D015723:Gene Library; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D012685:Sepharose / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014859:Warfarin / Q000031:analogs & derivatives
chemical_list: D004269:DNA, Bacterial; D004187:Disaccharides; D005697:Galactosides; D009844:Oligosaccharides; C071743:N-acetyl-gamma-glutamyl-4'-aminowarfarin; C081798:neoagarotetraose; C068544:neoagarobiose; D014859:Warfarin; D002244:Carbon; D012685:Sepharose; D006026:Glycoside Hydrolases; C019624:agarase
keywords: Cohnella sp. LGH; Endo-type β-agarase; Neoagarobiose; Neoagarotetraose