title: Epstein-Barr virus-encoded small RNA 1 (EBER-1) could predict good prognosis in nasopharyngeal carcinoma.
abstract: OBJECTIVE
  EBER-1 (a non-coding RNA transcribed by EBV) expression was detected in most of Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC) patients. However, the relevance between EBER-1 expression and NPC clinical outcome has not been reported. This study aims to assess the possible correlations of EBER-1 expression and clinical parameters and its potential prognostic predictive ability in NPC patient's outcomes.
  
  
  METHODS
  We examined EBER-1 mRNA expression in 301 NPC and 130 non-NPC tissues using in situ hybridization and did statistics.
  
  
  RESULTS
  EBER-1 expression was up-regulated in NPC tissues when compared to non-NPC tissues. A receiver operating characteristic analysis revealed that EBER-1 expression could distinguish non-cancerous patients from NPC patients (p < 0.001, sensitivity: 72.5 %, specificity: 83.5 %, AUC = 0.815). A survival analysis revealed that patients with high levels of EBER-1 expression had a significantly good prognosis (Disease-free survival: p = 0.019, overall survival: p = 0.006).
  
  
  CONCLUSIONS
  These results indicated that EBER-1 expression is a potential prognosis factor of NPC and highly negative correlated with the progress of NPC.
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China.|Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China.
authors.forename: S
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Medical Laboratory Science, Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Medical Laboratory Science, Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China.|Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China.|Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, Hunan, China.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
authors.forename: W
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, Hunan, China.|Mitchell Cancer Institute, University of South Alabama, Mobile, AL, 36604, USA.
authors.forename: M
authors.initials: M
authors.lastname: Tan
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China. zhangwenling73@126.com.|Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, Hunan, China. zhangwenling73@126.com.|Department of Medical Laboratory Science, Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China. zhangwenling73@126.com.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China. lgy@csu.edu.cn.|Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha, 410078, Hunan, China. lgy@csu.edu.cn.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China. lgy@csu.edu.cn.
authors.forename: G
authors.initials: G
authors.lastname: Li
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis*; D002277:Carcinoma; D018572:Disease-Free Survival; D020031:Epstein-Barr Virus Infections / Q000150:complications; D006801:Humans; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000401:mortality / Q000473:pathology* / Q000821:virology*; D011379:Prognosis; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral
keywords: EBV-encoded small RNA 1 (EBER-1); Epstein–Barr virus (EBV); Nasopharyngeal carcinoma (NPC); Prognosis