title: Low nourishment of B-vitamins is associated with hyperhomocysteinemia and oxidative stress in newly diagnosed cardiac patients.
abstract: We are currently witnessing a dramatic change in lifestyle and food choices that is accompanied with an increase in the rate of morbidity and mortality from cardiovascular diseases (CVD). Although studies have reported an association of CVD with hyperhomocysteinemia-mediated oxidative stress, the biochemical basis is not known. This case-control study was aimed to evaluate the nutritional and biochemical status of B-vitamins in relation to hyperhomocysteinemia and oxidative stress in newly diagnosed cardiac patients. The retrospective dietary intake of the study subjects (cases and controls) was estimated using a semi-quantitative food frequency questionnaire, and fasting blood samples were drawn to assess their serum levels of B-vitamins (folate, vitamins B6 and B12), homocysteine (HCY), and oxidative stress indices such as glutathione (GSH), total antioxidant capacity (TAC), malondialdehyde (MDA), and nitrites and nitrates (NN). It was observed that the cases had a lower dietary intake of B-vitamins as compared to their matched control subjects as well as to the corresponding recommended dietary allowances. Biochemical analysis of cases, as compared to controls, indicated depletion of GSH, impairment of TAC, and an elevation in the serum levels of HCY, MDA, and NN. These results suggest that lower status (dietary intake and serum levels) of B-vitamins is involved in the etiology of hyperhomocysteinemia and oxidative stress, the typical risk factors for CVD.
authors.affiliation: Food Science and Nutrition Department, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, 123, Sultanate of Oman Nutrition Department, High Institute of Public Health, Alexandria University, El-Hadrah, 165, Egypt waly.mostafa@gmail.com.
authors.forename: Mostafa I
authors.initials: MI
authors.lastname: Waly
authors.affiliation: Food Science and Nutrition Department, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, 123, Sultanate of Oman.
authors.forename: Amanat
authors.initials: A
authors.lastname: Ali
authors.affiliation: Nutrition Department, Royal Hospital, Ministry of Health, Muscat, 111, Sultanate of Oman.
authors.forename: Amira
authors.initials: A
authors.lastname: Al-Nassri
authors.affiliation: Cardiology and Cardiac Surgery Departments, Royal Hospital, Ministry of Health, Muscat, 111, Sultanate of Oman.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Al-Mukhaini
authors.affiliation: Cardiology and Cardiac Surgery Departments, Royal Hospital, Ministry of Health, Muscat, 111, Sultanate of Oman.
authors.forename: John
authors.initials: J
authors.lastname: Valliatte
authors.affiliation: Family Medicine and Public Health Department, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, 123, Sultanate of Oman.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Al-Farsi
mesh_terms: D000328:Adult; D000368:Aged; D001774:Blood Chemical Analysis; D002318:Cardiovascular Diseases / Q000150:complications*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020138:Hyperhomocysteinemia / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011795:Surveys and Questionnaires; D014804:Vitamin B Deficiency / Q000150:complications*
keywords: Folic acid; cardiac patients; hyperhomocysteinemia; oxidative stress; vitamin B12; vitamin B6