title: Association between interleukin 8-251 T/A and +781 C/T polymorphisms and glioma risk.
abstract: BACKGROUND
  Gliomas are aggressive tumors of the central nervous system that rely on production of growth factors for tumor progression. Interleukin 8 (IL-8) is up-regulated in gliomas to promote angiogenesis and proliferation. The aim of this study was to evaluate the association of the IL-8 -251 T/A and +781 C/T polymorphisms and glioma risk.
  
  
  METHODS
  We enrolled 300 glioma patients and 300 age- and gender-matched healthy controls. A prospective hospital-based case-control design and logistic regression analysis were utilized. The IL-8 gene polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  Glioma patients had a significantly higher frequency of IL-8 -251 AA genotype [odds ratio (OR) =1.91, 95% confidence interval (CI) = 1.22, 3.00; P = 0.005] and IL-8 -251 A allele (OR =1.36, 95% CI = 1.08, 1.70; P = 0.009) than controls. When stratified by the grade of glioma, patients with WHO IV glioma had a significantly higher frequency of IL-8 -251 AA genotype (OR =1.56, 95% CI = 1.01, 2.39; P = 0.04).
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first report in the literature that the IL-8 -251 AA genotype and A allele were at a higher risk for glioma.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao Tong University, West Yanta Road No.277, Xi'an, 710061, China. haoliujtu@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao Tong University, West Yanta Road No.277, Xi'an, 710061, China. pingmaojtu@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Mao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao Tong University, West Yanta Road No.277, Xi'an, 710061, China. changhouxie@163.com.
authors.forename: Changhou
authors.initials: C
authors.lastname: Xie
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao Tong University, West Yanta Road No.277, Xi'an, 710061, China. wanfuxiejtu@163.com.
authors.forename: Wanfu
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao Tong University, West Yanta Road No.277, Xi'an, 710061, China. maodewangjtu@163.com.
authors.forename: Maode
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao Tong University, West Yanta Road No.277, Xi'an, 710061, China. haitjiang@outlook.com.
authors.forename: Haitao
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D005910:Glioma / Q000235:genetics*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012307:Risk Factors
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8