title: Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk.
abstract: The coagulation of β-conglycinin (7S), glycinin (11S) and isoflavones induced by calcium chloride was investigated. Approximately 92.6% of the soymilk proteins were coagulated into the soymilk pellet fraction (SPF) after the addition of 5 mM calcium chloride. SDS-PAGE and two-dimensional electrophoresis analysis indicated that most of the 7S (α', α and β), 11S acidic (A1a, A1b, A2, A3 and A4) and 11S basic (B1a) proteins in the SSF were coagulated into the SPF after treatment with 5 mM calcium chloride. Isoflavones, including daidzein and genistein, were also coagulated into the SPF after the addition of 5 mM calcium chloride. The amounts of daidzein and genistein in the SSF decreased to 39.4 ± 1.6 and 11.8 ± 7.0%, respectively. HPLC analysis suggested that daidzein and genistein were bound with 7S and 11S proteins and then were coprecipitated into the SPF by 5 mM calcium chloride.
authors.affiliation: 1] Department of Food Science, Fu Jen Catholic University, Taipei 242, Taiwan [2] Ph.D. Program in Nutrition &Food Science, Fu Jen Catholic University, Taipei 242, Taiwan.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Hsiao
authors.affiliation: Department of Food Science, Fu Jen Catholic University, Taipei 242, Taiwan.
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Yu
authors.affiliation: National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 11221, Taiwan.
authors.forename: Wen-Tai
authors.initials: WT
authors.lastname: Li
authors.affiliation: 1] Department of Food Science, Fu Jen Catholic University, Taipei 242, Taiwan [2] Ph.D. Program in Nutrition &Food Science, Fu Jen Catholic University, Taipei 242, Taiwan.
authors.forename: Jung-Feng
authors.initials: JF
authors.lastname: Hsieh
mesh_terms: D052179:Antigens, Plant / Q000737:chemistry* / Q000378:metabolism; D002122:Calcium Chloride / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019833:Genistein / Q000737:chemistry / Q000378:metabolism; D005916:Globulins / Q000737:chemistry* / Q000378:metabolism; D007529:Isoflavones / Q000737:chemistry* / Q000378:metabolism; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000378:metabolism; D041942:Soy Milk / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D007529:Isoflavones; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C004742:daidzein; C011474:glycinin; D019833:Genistein; D002122:Calcium Chloride