title: A Novel Member of GH16 Family Derived from Sugarcane Soil Metagenome.
abstract: Glycoside hydrolases (GHs) are enzymes found in all living kingdoms that are involved in multiple physiological functions. Due to their multiple enzymatic activities, GHs are broadly applied in bioethanol, food, and paper industry. In order to increase the productivity of these industrial processes, a constant search for novel and efficient enzymes has been proved to be necessary. In this context, metagenomics is a powerful approach to achieve this demand. In the current study, we describe the discovery and characterization of a novel member of GH16 family derived from the sugarcane soil metagenome. The enzyme, named SCLam, has 286 amino acid residues and displays sequence homology and activity properties that resemble known laminarases. SCLam is active against barley beta-glucan, laminarin, and lichenan (72, 33, and 10 U mg(-1), respectively). The optimal reaction conditions were identified as 40 °C and pH 6.5. The low-resolution structure was determined using the small-angle X-ray scattering technique, revealing that SCLam is a monomer in solution with a radius of gyration equal to 19.6 Å. To the best of our knowledge, SCLam is the first nonspecific (1,3/1,3:1,4)-β-D-glucan endohydrolase (EC 3.2.1.6) recovered by metagenomic approach to be characterized.
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, SP, 13083-100, Brazil, thabata.alvarez@bioetanol.org.br.
authors.forename: Thabata Maria
authors.initials: TM
authors.lastname: Alvarez
authors.forename: Marcelo Vizoná
authors.initials: MV
authors.lastname: Liberato
authors.forename: João Paulo L Franco
authors.initials: JP
authors.lastname: Cairo
authors.forename: Douglas A A
authors.initials: DA
authors.lastname: Paixão
authors.forename: Bruna M
authors.initials: BM
authors.lastname: Campos
authors.forename: Marcel R
authors.initials: MR
authors.lastname: Ferreira
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Almeida
authors.forename: Isabela O
authors.initials: IO
authors.lastname: Pereira
authors.forename: Amanda
authors.initials: A
authors.lastname: Bernardes
authors.forename: Gabriela C G
authors.initials: GC
authors.lastname: Ematsu
authors.forename: Mariana
authors.initials: M
authors.lastname: Chinaglia
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
authors.forename: Mario
authors.initials: M
authors.lastname: de Oliveira Neto
authors.forename: Fabio Marcio
authors.initials: FM
authors.lastname: Squina
mesh_terms: D000595:Amino Acid Sequence; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D054892:Metagenome; D010802:Phylogeny; D031786:Saccharum / Q000254:growth & development*; D053838:Scattering, Small Angle; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D006026:Glycoside Hydrolases