title: Production of anti-CD3/28 expanded CD4⁺ T lymphocytes from HIV-infected patients with different degrees of disease progression.
abstract: OBJECTIVE
  CD4+ T lymphocytes from HIV-infected patients with different degrees of disease progression based on CD4 count were expanded in vitro using anti-CD3/28-coated beads.
  
  
  METHODS
  Purified CD4+ T lymphocytes from healthy subjects and patients were expanded for 3 weeks. Moreover, the improvement of cell expansion by IL-2 supplementation was also determined.
  
  
  RESULTS
  Expanded CD4+ T lymphocytes from patients had lower fold expansion when compared with healthy subjects. Furthermore, patients with high CD4 counts had higher fold expansion than patients with low CD4 count, and IL-2 supplementation further increased cell expansion.
  
  
  CONCLUSIONS
  Anti-CD3/28 activation failed to potently induce expansion of CD4+ T lymphocytes from patients. However, the cell expansion could be improved by IL-2 supplementation.
authors.affiliation: Department of Research & Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.|Center for Emerging & Neglected Infectious Diseases, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Nattawat
authors.initials: N
authors.lastname: Onlamoon
authors.affiliation: Department of Research & Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.|Master of Science Program in Immunology, Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Vajee
authors.initials: V
authors.lastname: Petphong
authors.affiliation: Department of Research & Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Kasama
authors.initials: K
authors.lastname: Sukapirom
authors.affiliation: Department of Research & Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.|Master of Science Program in Immunology, Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Siyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Transfusion Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Palanee
authors.initials: P
authors.lastname: Ammaranond
authors.affiliation: Department of Research & Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.|Center for Emerging & Neglected Infectious Diseases, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Kovit
authors.initials: K
authors.lastname: Pattanapanyasat
mesh_terms: D000328:Adult; D064591:Allografts; D018106:CD28 Antigens; D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000637:transplantation*; D005260:Female; D015658:HIV Infections / Q000276:immunology / Q000628:therapy*; D006801:Humans; D017710:Lymphocyte Transfusion; D008297:Male; D008875:Middle Aged
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex
keywords: CD4+ T lymphocytes; HIV-infected patients; anti-CD3/28 coated beads; in vitro cell expansion