title: Precocene II, a Trichothecene Production Inhibitor, Binds to Voltage-Dependent Anion Channel and Increases the Superoxide Level in Mitochondria of Fusarium graminearum.
abstract: Precocene II, a constituent of essential oils, shows antijuvenile hormone activity in insects and inhibits trichothecene production in fungi. We investigated the molecular mechanism by which precocene II inhibits trichothecene production in Fusarium graminearum, the main causal agent of Fusarium head blight and trichothecene contamination in grains. Voltage-dependent anion channel (VDAC), a mitochondrial outer membrane protein, was identified as the precocene II-binding protein by an affinity magnetic bead method. Precocene II increased the superoxide level in mitochondria as well as the amount of oxidized mitochondrial proteins. Ascorbic acid, glutathione, and α-tocopherol promoted trichothecene production by the fungus. These antioxidants compensated for the inhibitory activity of precocene II on trichothecene production. These results suggest that the binding of precocene II to VDAC may cause high superoxide levels in mitochondria, which leads to stopping of trichothecene production.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Furukawa
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Sakamoto
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Sakuda
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001578:Benzopyrans / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005978:Glutathione / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D013481:Superoxides / Q000819:agonists / Q000378:metabolism; D014255:Trichothecenes / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D050994:Voltage-Dependent Anion Channels / Q000235:genetics / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D001578:Benzopyrans; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D014255:Trichothecenes; D050994:Voltage-Dependent Anion Channels; D013481:Superoxides; C014163:precocene II; D005978:Glutathione; D024502:alpha-Tocopherol; D001205:Ascorbic Acid