title: Interaction of Virus-Like Particles with Vesicles Containing Glycolipids: Kinetics of Detachment.
abstract: Many viruses interact with their host cells via glycosphingolipids (GSLs) and/or glycoproteins present on the outer cell membrane. This highly specific interaction includes virion attachment and detachment. The residence time determined by the detachment is particularly interesting, since it is directly related to internalization and infection as well as to virion egress and spreading. In an attempt to deepen the understanding of virion detachment kinetics, we have used total internal reflection fluorescence (TIRF) microscopy to probe the interaction between individual fluorescently labeled GSL-containing lipid vesicles and surface-bound virus-like particles (VLPs) of a norovirus genotype II.4 strain. The distribution of the VLP-vesicle residence time was investigated for seven naturally occurring GSLs, all of which are candidates for the not yet identified receptor(s) mediating norovirus entry into host cells. As expected for interactions involving multiple GSL binding sites at a viral capsid, the detachment kinetics displayed features typical for a broad activation-energy distribution for all GSLs. Detailed inspection of these distributions revealed significant differences among the different GSLs. The results are discussed in terms of strength of the interaction, vesicle size, as well as spatial distribution and clustering of GSLs in the vesicle membrane.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg, Sweden.
authors.forename: Waqas
authors.initials: W
authors.lastname: Nasir
authors.affiliation: Department of Applied Physics, Chalmers University of Technology , SE-412 96 Gothenburg, Sweden.|Institut Curie, Centre de Recherche, CNRS, UMR 168, Physico-Chimie Curie, F-75248 Paris, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Bally
authors.affiliation: Department of Applied Physics, Chalmers University of Technology , SE-412 96 Gothenburg, Sweden.|Boreskov Institute of Catalysis, Russian Academy of Sciences , Novosibirsk 630090, Russia.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Zhdanov
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Larson
authors.affiliation: Department of Applied Physics, Chalmers University of Technology , SE-412 96 Gothenburg, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Höök
mesh_terms: D002213:Capsid / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry*; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D029322:Norovirus / Q000737:chemistry*
chemical_list: D006028:Glycosphingolipids; D008051:Lipid Bilayers