title: MALAT1-miR-124-RBG2 axis is involved in growth and invasion of HR-HPV-positive cervical cancer cells.
abstract: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT 1) is a large, infrequently spliced non-coding RNA aberrantly expressed in cervical cancer. But the molecular mechanisms of its oncogenic role are still not quite clear. The present study explored whether there is a competing endogenous RNAs (ceRNAs) mechanism involved in the oncogenic effect of MALAT1. MALAT1 expression was firstly verified in high-risk human papillomavirus (HR-HPV)-positive tumor tissues and cell lines. Its regulation over miR-124 and the downstream target of miR-124 in regulation of growth, invasion, and apoptosis of the cancer cells are also studied. Findings of this study confirmed higher MALAT1 expression in HR-HPV (+) cervical cancer. Knockdown of endogenous MALAT1 significantly reduced cell growth rate and invasion and increased cell apoptosis of Hela and siHa cells. Besides, knockdown of MALAT1 increased the expression of miRNA-124, while ectopic expression of miR-124 decreased MALAT1 expression. In addition, we also verified a direct interaction between miR-124 and 3'UTR of GRB2. MALAT1 can indirectly modulate GRB2 expression via competing miR-124. Knockdown of GRB2 reduced cell invasion and increased cell apoptosis. In conclusion, MALAT1 can promote HR-HPV (+) cancer cell growth and invasion at least partially through the MALAT1-miR-124-RBG2 axis. This finding might provide some useful evidence about the lncRNA interaction regulatory network in tumorigenesis cervical cancer.
authors.affiliation: Cangzhou Central Hospital, No. 16, Xinhua West Road, Canal Zone, Cangzhou City, Hebei Province, 061001, China.
authors.forename: Shikai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Cangzhou Central Hospital, No. 16, Xinhua West Road, Canal Zone, Cangzhou City, Hebei Province, 061001, China. commasll@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Song
authors.affiliation: Cangzhou Central Hospital, No. 16, Xinhua West Road, Canal Zone, Cangzhou City, Hebei Province, 061001, China.
authors.forename: Saitian
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Cangzhou Central Hospital, No. 16, Xinhua West Road, Canal Zone, Cangzhou City, Hebei Province, 061001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D051380:GRB2 Adaptor Protein / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000209:etiology* / Q000473:pathology*
chemical_list: D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; C557041:MALAT1 long non-coding RNA, human; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
keywords: Cervical cancer; GRB2; HR-HPV; MALAT1; miR-124