title: Analysis of TTSuV1b antibody in porcine serum and its correlation with four antibodies against common viral infectious diseases.
abstract: BACKGROUND
  The purpose of the present study was to evaluate the correlation between Torque teno sus virus 1b (TTSuV1b) infection and other viral infections or vaccine immunization in conventional pigs.
  
  
  METHODS
  With overexpressed and purified viral protein TTSuV1b as antigen, an indirect enzyme-linked immunosorbent assay (ELISA) method for detecting TTSuV1b antibody was established, which demonstrated great specificity and reproducibility. Porcine serum samples (n = 212) were tested using ELISA. Meanwhile, the antibodies against Classical Swine Fever Virus (CSFV), Pseudorabies Virus (PRV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and Porcine Circovirus 2 (PCV2) were also examined using the commercial kits.
  
  
  RESULTS
  Statistical analysis indicated that the level of anti-TTSuV1b antibody was positively correlated with the level of anti-PCV2 antibody in a lesser extent; the level of antibodies against TTSuV1b or PCV2 were significantly lower in porcine serum with low level of TTSuV1b virus, implicating the potential consistency and synchronization in the mechanism of TTSuV1b and PCV2 infection. Whereas, antibodies against PRRSV or CSFV showed no statistical significance on comparison with anti-TTSuV1b antibody, implicating that in conventional pigs, the antibody level for PRRSV and CSFV were not significantly influenced by TTSuV1b infection.
  
  
  CONCLUSIONS
  In conclusion, examination of anti-TTSuV1b antibody in porcine serum with the presently established ELISA method would serve as a supplementary approach for etiological investigation, and the combined statistical analysis of the antibodies against four other viruses might help to further understand the TTSuV1b infection as well as its pathogenicity.
authors.affiliation: Department of veterinary research, Guangdong Haid Institute of Animal Husbandry and Veterinary, Panyu District, Fuping Road, Guangzhou, 511440, China. lzs-jason@hotmail.com.
authors.forename: Zhongsheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of veterinary research, Guangdong Haid Institute of Animal Husbandry and Veterinary, Panyu District, Fuping Road, Guangzhou, 511440, China. qiaojx@haid.com.cn.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Department of veterinary research, Guangdong Haid Institute of Animal Husbandry and Veterinary, Panyu District, Fuping Road, Guangzhou, 511440, China. heyl2@haid.com.cn.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of veterinary research, Guangdong Haid Institute of Animal Husbandry and Veterinary, Panyu District, Fuping Road, Guangzhou, 511440, China. lizs@haid.com.cn.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of veterinary research, Guangdong Haid Institute of Animal Husbandry and Veterinary, Panyu District, Fuping Road, Guangzhou, 511440, China. wanggp2@haid.com.cn.
authors.forename: Guiping
authors.initials: G
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins