title: A rapid MZI-IDA sensor system for EGFR mutation testing in non-small cell lung cancer (NSCLC).
abstract: Epidermal growth factor receptor (EGFR) is a non-small-cell lung cancer biomarker, based on which several near-patient-testing methods have been developed and applied to predict treatment response on individual patients. Existing methods for detection of EGFR mutation are costly, labor-intensive and time-consuming. In this paper, we report a novel EGFR mutation testing system, which is based on Mach-Zehnder Interferometer (MZI) sensor and isothermal solid-phase DNA amplification (IDA) technique, called MZI-IDA sensor system. The system can deliver results within 30 min and shows high sensitivity to detect trace amounts of genomic DNA (<1 copy). In addition, the system is able to detect a L858R mutation in a 99:1 mixture of wild-type and mutant cells. In a pilot clinical study, the system is compared with conventional methods (PCR and direct sequencing) by using tissue biopsy samples from NSCLC patients. The MZI-IDA sensor system is proved to be capable of fast and accurate detection of the L858R mutation of EGFR gene in clinical samples. This may greatly help the clinicians develop an appropriate treatment plan.
authors.affiliation: Institute of Microelectronics, A⁎STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685 Singapore.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Microelectronics, A⁎STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685 Singapore.
authors.forename: Swee Yin
authors.initials: SY
authors.lastname: Lim
authors.affiliation: Department of Hematology-Oncology, National University Cancer Institute of Singapore, Singapore; National University Health System, Singapore; Cancer Science Institute of Singapore, Singapore; National University of Singapore, Singapore.
authors.forename: Ross A
authors.initials: RA
authors.lastname: Soo
authors.affiliation: Institute of Microelectronics, A⁎STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685 Singapore.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Park
authors.affiliation: Institute of Microelectronics, A⁎STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685 Singapore; Asan Institute for Life Science, Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul, Korea. Electronic address: shiny@ime.a-star.edu.sg.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shin
mesh_terms: D015374:Biosensing Techniques; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics / Q000302:isolation & purification*; D005091:Exons; D005838:Genotype; D006801:Humans; D007368:Interferometry; D019169:Jurkat Cells; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics*; D009154:Mutation
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Epidermal growth factor receptor; Isothermal DNA amplification; Mach Zehnder interferometer sensor; Mutation testing; Non-Small cell lung cancer