title: Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton's Jelly into Induced Pluripotent Stem Cells.
abstract: In an attempt to bring pluripotent stem cell biology closer to reaching its full potential, many groups have focused on improving reprogramming protocols over the past several years. The episomal modified Sendai virus-based vector has emerged as one of the most practical ones. Here we describe reprogramming of mesenchymal stromal/stem cells (MSC) derived from umbilical cord Wharton's Jelly into induced pluripotent stem cells (iPSC) using genome non-integrating Sendai virus-based vectors. The detailed protocols of iPSC colony cryopreservation (vitrification) and adaption to feeder-free culture conditions are also included.
authors.affiliation: Stem Cell Laboratories, Assisted Conception Unit, Guy's Hospital, London, UK.
authors.forename: Cristian
authors.initials: C
authors.lastname: Miere
authors.affiliation: Stem Cell Laboratories, Assisted Conception Unit, Guy's Hospital, London, UK.
authors.forename: Liani
authors.initials: L
authors.lastname: Devito
authors.affiliation: Stem Cell Laboratories, Assisted Conception Unit, Guy's Hospital, London, UK.|Kings College London, Strand, London, WC2R2LS, UK.
authors.forename: Dusko
authors.initials: D
authors.lastname: Ilic
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D029082:Sendai virus / Q000235:genetics*; D014470:Umbilical Cord / Q000166:cytology; D058989:Vitrification; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D003470:Culture Media
keywords: Adaption to feeder-free culture conditions; MSC; Reprogramming; Sendai virus; Umbilical cord; Vitrification; iPSC