title: Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.
abstract: Glia Maturation Factor-β (GMF), a brain specific protein, is induced by proteinuria in renal tubules. Ectopic GMF overexpression causes apoptosisin vitro via cellular vulnerability to oxidative stress. In order to examine the roles of GMF in non-brain tissue, we constructed transgenic mice overexpressing GMF (GMF-TG). The GMF-TG mice exhibited appearance phenotypes associated with premature aging. The GMF-TG mice also demonstrated short lifespans and reduced hair regrowth, suggesting an accelerated aging process. The production of an abnormal lamin A, a nuclear envelope protein, plays a causal role in both normal aging and accelerated aging diseases, known as laminopathies. Importantly, we identified the abnormal lamin A (prelamin A), accompanied by a down-regulation of a lamin A processing enzyme (Zmpste24) in the kidney of the GMF-TG mice. The GMF-TG mice showed accelerated aging in the kidney, compared with wild-type mice, showing increased TGF-β1, CTGF gene and serum creatinine. The gene expression of p21/waf1 was increased at an earlier stage of life, at 10 weeks, which was in turn down-regulated at a later stage, at 60 weeks. In conclusion, we propose that GMF-TG mice might be a novel mouse model of accelerated aging, due to the abnormal lamin A.
authors.affiliation: Clinical Nutrition and Internal Medicine, Kobe Women's University, Kobe 654-8585, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Imai
authors.affiliation: Clinical Nutrition and Internal Medicine, Kobe Women's University, Kobe 654-8585, Japan.|Current address: Department of Metabolism and Clinical Nutrition, Kyoto University Hospital, Kyoto 606-8507, Japan.
authors.forename: Kanae
authors.initials: K
authors.lastname: Asai
authors.affiliation: Renal Division and Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hanai
authors.affiliation: Clinical Nutrition and Internal Medicine, Kobe Women's University, Kobe 654-8585, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Takenaka
mesh_terms: D000375:Aging / Q000235:genetics; D019588:Aging, Premature / Q000235:genetics*; D000818:Animals; D003404:Creatinine / Q000097:blood; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects; D020904:Glia Maturation Factor / Q000096:biosynthesis / Q000235:genetics*; D006197:Hair / Q000254:growth & development; D053208:Kaplan-Meier Estimate; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism
chemical_list: D020904:Glia Maturation Factor; D034904:Lamin Type A; D008565:Membrane Proteins; D003404:Creatinine; D008666:Metalloendopeptidases; C491250:Zmpste24 protein, mouse; D016283:Proto-Oncogene Proteins p21(ras)
keywords: Zmpste24; aging; glia maturation factor-β; oxidative stress; prelamin A