title: The Epidemiological Characteristics of Beijing Lineage Mycobacterium tuberculosis from a National Referral Center in China.
abstract: Our study was to investigate the epidemiological characteristics of M.tuberculosis from a national tuberculosis referral center in China. All strains isolated from TB patients, were genotyped by the RD105 deletion, 8 and 51 SNP loci and VNTR. The high differentiation SNPs of modern Beijing strains were analyzed for protein function and structure. 413 M. tuberculosis were included. Of 379 Beijing lineage M. tuberculosis, 'modern' and 'ancient' strains respectively represented 85.5% (324/379) and 14.5% (55/379). Rv2494 (V48A) and Rv0245 (S103F) were confirmed as high differentiation SNPs associated with modern strains. In a word, Modern Beijing lineage M.tuberculosis was dominant and the structural models suggested that modern sub-lineage may more easily survive in 'extreme' host condition.
authors.affiliation: Tianjin Children's Hospital, Tianjin 300074, China.
authors.forename: Xiao Ying
authors.initials: XY
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Clinical Epidemiology Unit, Peking Union Medical College, Beijing 101149, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Wan Li
authors.initials: WL
authors.lastname: Kang
authors.affiliation: National Tuberculosis Clinical Lab of China, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China; Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Zhao
authors.affiliation: National Tuberculosis Clinical Lab of China, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Peng Ju
authors.initials: PJ
authors.lastname: Ding
authors.affiliation: Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wen Tao
authors.initials: WT
authors.lastname: Dai
authors.affiliation: National Tuberculosis Clinical Lab of China, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China; Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Hai Rong
authors.initials: HR
authors.lastname: Huang
authors.affiliation: Division of Infectious Disease and Gastroenterology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
authors.forename: Yan Feng
authors.initials: YF
authors.lastname: Huang
authors.affiliation: National Tuberculosis Clinical Lab of China, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China; Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Wei Min
authors.initials: WM
authors.lastname: Li
mesh_terms: D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006763:Hospitals, Chronic Disease; D006801:Humans; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D058974:Phylogeography; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial