title: Structure of human collapsin response mediator protein 1: a possible role of its C-terminal tail.
abstract: Collapsin response mediator protein 1 (CRMP-1) is the first identified member of the CRMP family and is crucial for both the mediation of neuronal differentiation and in suppressing the invasion of lung cancer. The crystal structure of full-length human CRMP-1 was determined at a resolution of 3 Å. Human CRMP-1 comprises a tetrameric assembly; its overall structure is similar to that of mouse CRMP-1, but the measured electron density of the C-terminal residues 488-496 show a randomly coiled link that connects the protomers to each other, within which residues 497-572 are proteolytically susceptible in vivo. Deletion of residues 472-572 by thrombin in vitro not only releases a randomly coiled tail but also transduces observable structural changes of CRMP-1, as revealed by analytical size-exclusive chromatography and circular dichroism spectra. These results indicate a possible alternative role in CRMP dynamics and function.
authors.affiliation: Life science group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Szu Heng
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Facility Utilization Group, Experimental Facility Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Shih Fang
authors.initials: SF
authors.lastname: Huang
authors.affiliation: Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Yuan Ling
authors.initials: YL
authors.lastname: Hsu
authors.affiliation: Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Szu Hua
authors.initials: SH
authors.lastname: Pan
authors.affiliation: Life science group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yen Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Life science group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yi Hung
authors.initials: YH
authors.lastname: Lin
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D040681:Structural Homology, Protein; D013917:Thrombin / Q000737:chemistry; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D013917:Thrombin; C102924:CRMP1 protein, human; D000098855:Collapsin Response Mediator Protein 1; C502872:Crmp1 protein, mouse
keywords: circular dichoism; collapsin response mediator protein 1; crystal structure; lung cancer suppressor; non-small-cell lung cancer