title: Preparation of bio-deep eutectic solvent triggered cephalopod shaped silver chloride-DNA hybrid material having antibacterial and bactericidal activity.
abstract: 2.5% w/w DNA (Salmon testes) was solubilized in a bio-deep eutectic solvent [(bio-DES), obtained by the complexation of choline chloride and ethylene glycol at 1:2 molar ratio] containing 1% w/w of silver chloride (AgCl) to yield a AgCl decorated DNA based hybrid material. Concentration dependent formation of AgCl crystals in the DES was observed and upon interaction with DNA it gave formation of a cephalopod shaped hybrid material. DNA was found to maintain its chemical and structural stability in the material. Further, AgCl microstructures were found to have orderly self assembled on the DNA helices indicating the electrostatic interaction between Ag(+) and phosphate side chain of DNA as a driving force for the formation of the material with ordered microstructural distribution of AgCl. Furthermore, the functionalized material exhibited excellent antibacterial and bactericidal activity against both Gram negative and Gram positive pathogenic bacteria.
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India; AcSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India.
authors.forename: Jitkumar
authors.initials: J
authors.lastname: Bhatt
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India; AcSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India.
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Mondal
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India; AcSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Bhojani
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India; AcSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India. Electronic address: kamlesh@csmcri.org.
authors.forename: Kamalesh
authors.initials: K
authors.lastname: Prasad
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D000900:Anti-Bacterial Agents; D010710:Phosphates; D018030:Silver Compounds; D012997:Solvents; D012834:Silver; D004247:DNA; C037548:silver chloride
keywords: Antibacterial; Bactericidal activity; Bio-deep eutectic solvent; Cephalopod like architecture; DNA