title: Response of indigenously developed bacterial consortia in progressive degradation of polyvinyl chloride.
abstract: Thermoplastic-based materials are recalcitrant in nature, which extensive use affect environmental health. Here, we attempt to compare the response of indigenously produced bacterial consortium-I and consortium-II in degrading polyvinyl chloride (PVC). These consortia were developed by using different combination of bacterial strains of Pseudomonas otitidis, Bacillus cereus, and Acanthopleurobacter pedis from waste disposal sites of Northern India after their identification via 16S rDNA sequencing. The progressive degradation of PVC by consortia was examined via scanning electron microscopy, atomic force microscopy, UV-vis, FT-IR spectra, gel permeation chromatography, and differential scanning calorimetry analysis at different incubations and time intervals. The consortium-II was superior over consortium-I in degrading the PVC. Further, the carbon source utilization analysis revealed that the extensive use of consortia has not any effect on functional diversity of native soil microbes.
authors.affiliation: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India. shahbazmicro@gmail.com.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Anwar
authors.affiliation: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kapri
authors.affiliation: Division of Plant-Microbe Interactions, National Botanical Research Institute, R.P. Marg, Lucknow, 226001, India.
authors.forename: Vasvi
authors.initials: V
authors.lastname: Chaudhry
authors.affiliation: Division of Plant-Microbe Interactions, National Botanical Research Institute, R.P. Marg, Lucknow, 226001, India.
authors.forename: Aradhana
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Zakir Husain Delhi College, Jawahar Lal Nehru Marg, New Delhi, 110002, India.
authors.forename: Mohammad W
authors.initials: MW
authors.lastname: Ansari
authors.affiliation: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.|National Centre for Cell Sciences, Pune University Campus, Ganeskhind, Pune, 411007, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Souche
authors.affiliation: Division of Plant-Microbe Interactions, National Botanical Research Institute, R.P. Marg, Lucknow, 226001, India.
authors.forename: Chandra S
authors.initials: CS
authors.lastname: Nautiyal
authors.affiliation: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.
authors.forename: M G H
authors.initials: MG
authors.lastname: Zaidi
authors.affiliation: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India. rg55@rediffmail.com.
authors.forename: Reeta
authors.initials: R
authors.lastname: Goel
mesh_terms: D001673:Biodegradation, Environmental; D059013:Microbial Consortia / Q000235:genetics*; D058889:Molecular Typing; D011143:Polyvinyl Chloride / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D011143:Polyvinyl Chloride
keywords: In situ biodegradation; Indigenous consortia; Microbial community; Polyvinyl chloride