title: Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta.
abstract: Laminaran and three fractions of fucoidan were isolated from brown alga Alaria angusta. The laminaran AaL was characterized as a typical 1,3;1,6-β-D-glucan (ratio of bonds 1,3:1,6 = 10:1). Fucoidans AaF1 and AaF2 are sulfated heteropolysaccharides, containing fucose, galactose, mannose and xylose. The fraction AaF3 is sulfated and acetylated galactofucan with the main chain represented by a repeating unit → 3)-α-L-Fucp-(2,4-SO3(-))-(1 →. According the data of methylation analysis, AaF3 contains mainly 1,3-linked fucose, less 1,4-linked and 1,4,6-linked galactose residues. The autohydrolysis (37 °C) of fucoidan AaF3 allowed to obtain selectively 2-desulfaled polysaccharide fraction, built up of fucose only, and low molecular weight (LMW) fraction. The negative-ion tandem mass spectrometry of LMW fraction, further hydrolyzed by acid hydrolysis identified the following fragments: Gal-2-SO3(-)-(1 → 4)-Gal, Gal-4-SO3(-)-(1 → 4)-Gal, Gal-(1 → 2)-Gal-4-SO3(-), Fuc-2-SO3(-)-(1 → 4)-Gal, Gal-2-SO3(-)-(1 → 3)-Fuc-(1 → 3)-Fuc, Fuc-2-SO3(-)-(1 → 3)-Fuc-(1 → 4)-Gal. The laminaran AaL and the fucoidan AaF3 exhibited no cytotoxicity in vitro for HT 29, T-47D, and SK-MEL-28 cell lines. The AaF3 fraction suppressed colony formation of HT 29 and T-47D cells, AaL-only HT 29 cells.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022 Vladivostok, Russian Federation. Electronic address: Menshova-R@yandex.ru.
authors.forename: Roza V
authors.initials: RV
authors.lastname: Menshova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Anastyuk
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Ermakova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Shevchenko
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Isakov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D005936:Glucans; D011134:Polysaccharides; C008247:laminaran; C007789:fucoidan
keywords: Anticancer activity; Brown alga; Depolymerization; Fucoidan; Structure