title: Genetic Alterations in Hungarian Patients with Papillary Thyroid Cancer.
abstract: The incidence of thyroid cancers is increasing worldwide. Some somatic oncogene mutations (BRAF, NRAS, HRAS, KRAS) as well as gene translocations (RET/PTC, PAX8/PPAR-gamma) have been associated with the development of thyroid cancer. In our study, we analyzed these genetic alterations in 394 thyroid tissue samples (197 papillary carcinomas and 197 healthy). The somatic mutations and translocations were detected by Light Cycler melting method and Real-Time Polymerase Chain Reaction techniques, respectively. In tumorous samples, 86 BRAF (44.2%), 5 NRAS (3.1%), 2 HRAS (1.0%) and 1 KRAS (0.5%) mutations were found, as well as 9 RET/PTC1 (4.6%) and 1 RET/PTC3 (0.5%) translocations. No genetic alteration was seen in the non tumorous control thyroid tissues. No correlation was detected between the genetic variants and the pathological subtypes of papillary cancer as well as the severity of the disease. Our results are only partly concordant with the data found in the literature.
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary. tobias.balint@med.semmelweis-univ.hu.
authors.forename: Bálint
authors.initials: B
authors.lastname: Tobiás
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Halászlaki
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Bernadett
authors.initials: B
authors.lastname: Balla
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.|PentaCore Laboratory, Budapest, Hungary.
authors.forename: János P
authors.initials: JP
authors.lastname: Kósa
authors.affiliation: PentaCore Laboratory, Budapest, Hungary.
authors.forename: Kristóf
authors.initials: K
authors.lastname: Árvai
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Horváth
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Takács
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Nagy
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Evelin
authors.initials: E
authors.lastname: Horváth
authors.affiliation: 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Horányi
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Járay
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Székely
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Székely
authors.affiliation: Department of Radiology and Oncotherapy, Semmelweis University, Budapest, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Győri
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Putz
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Magdolna
authors.initials: M
authors.lastname: Dank
authors.affiliation: Department of Medicine, University of Szeged, Szeged, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Valkusz
authors.affiliation: Department of Pathology, University of Szeged, Szeged, Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Vasas
authors.affiliation: Department of Pathology, University of Szeged, Szeged, Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Iványi
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Korányi S. Str. 2a, Budapest, 1083, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Lakatos
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D018631:ras Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D018631:ras Proteins
keywords: BRAF; Mutation; Papillary thyroid cancer; RAS; RET/PTC