title: SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction.
abstract: BACKGROUND
  The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites.
  
  
  METHODS
  Here we investigated how SDF-1α could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases.
  
  
  RESULTS
  We show that different concentrations of SDF-1α modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100 ng/ml of SDF-1α; however migration was reduced at 200 ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1α treatment at 200 ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1α concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1α mediating-cell adhesion.
  
  
  CONCLUSIONS
  We conclude that SDF-1α concentration modulates migration and adhesion of breast cancer cells, by controlling expression and activation of RhoGTPases.
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. jep2026@qatar-med.cornell.edu.|Department of Genetic Medicine, Weill Cornell Medical College, New York, NY, USA. jep2026@qatar-med.cornell.edu.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Pasquier
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. naa2037@qatar-med.cornell.edu.
authors.forename: Nadine
authors.initials: N
authors.lastname: Abu-Kaoud
authors.affiliation: Department of Immunology and Microbiology, Weill Cornell Medical College in Qatar, Qatar Foundation, Education city, P.O. Box: 24144, Doha, Qatar. hoa2004@qatar-med.cornell.edu.
authors.forename: Houari
authors.initials: H
authors.lastname: Abdesselem
authors.affiliation: Department of Immunology and Microbiology, Weill Cornell Medical College in Qatar, Qatar Foundation, Education city, P.O. Box: 24144, Doha, Qatar. aym2003@qatar-med.cornell.edu.
authors.forename: Aisha
authors.initials: A
authors.lastname: Madani
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. jeh2036@qatar-med.cornell.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Hoarau-Véchot
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. res-te2@qatar-med.cornell.edu.
authors.forename: Hamda Al
authors.initials: HA
authors.lastname: Thawadi
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. dox_94@yahoo.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Vidal
authors.affiliation: EA 4553, Institut Claudius Regaud, Toulouse, France. Couderc.Bettina@claudiusregaud.fr.
authors.forename: Bettina
authors.initials: B
authors.lastname: Couderc
authors.affiliation: INSERM U1037 Cancer Research Center of Toulouse, Institut Claudius Regaud, Toulouse, France. favre.gilles@claudiusregaud.fr.
authors.forename: Gilles
authors.initials: G
authors.lastname: Favre
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar. jat2021@qatar-med.cornell.edu.|Department of Genetic Medicine, Weill Cornell Medical College, New York, NY, USA. jat2021@qatar-med.cornell.edu.|Department of Advanced gynecologic Surgery, Université Montpellier 1, Montpellier, France. jat2021@qatar-med.cornell.edu.|Department of Genetic Medicine and Obstetrics and Gynecology, Stem cell and microenvironment laboratory Weill Cornell Medical College in Qatar, Qatar-Foundation, PO: 24144, Doha, Qatar. jat2021@qatar-med.cornell.edu.
authors.forename: Arash
authors.initials: A
authors.lastname: Rafii
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism / Q000494:pharmacology*; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C107953:RAC1 protein, human; C066737:RHOA protein, human; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein