title: Simultaneous Determination of Trigonelline, Caffeine, Chlorogenic Acid and Their Related Compounds in Instant Coffee Samples by HPLC Using an Acidic Mobile Phase Containing Octanesulfonate.
abstract: In order to analyze trigonelline, caffeine, chlorogenic acid, and their related compounds simultaneously, an HPLC method using an InertSustain C18 column and a mobile phase containing octanesulfonate as an ion-pairing reagent under an acidic condition was developed. The optimum mobile phase conditions were determined to be 0.1% phosphoric acid, 4 mM octanesulfonate, and 15% methanol at 35°C. Using the proposed method, trigonelline, nicotinic acid, caffeine, theophylline, chlorogenic acid, and caffeic acid in ten instant coffee samples were analyzed. These analytes except for theophylline were detected in all samples. An increase in the caffeine content in instant coffee samples tended to decrease in both trigonelline and chlorogenic acid contents, and the trigonelline content was found to be correlated well with the chlorogenic acid content (R(2) = 0.887).
authors.affiliation: School of Science, Tokai University.
authors.forename: Kana
authors.initials: K
authors.lastname: Arai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Terashima
authors.forename: Sen-ichi
authors.initials: S
authors.lastname: Aizawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Taga
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Kaname
authors.initials: K
authors.lastname: Tsutsumiuchi
authors.forename: Shuji
authors.initials: S
authors.lastname: Kodama
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D017738:Alkanesulfonic Acids / Q000737:chemistry*; D002110:Caffeine / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002726:Chlorogenic Acid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000470:Alkaloids; D017738:Alkanesulfonic Acids; D003069:Coffee; D002726:Chlorogenic Acid; D002110:Caffeine; C009560:trigonelline; C042005:1-octanesulfonic acid