title: [Effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels induced by overexpression or mutation of α-synuclein].
abstract: OBJECTIVE
  To investigate the effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels (mitoKATP) induced by overexpression or mutation of α-synuclein.
  
  
  METHODS
  Recombinant plasmids α-synuclein-pEGFP-A53T and α-synuclein-pEGFP-WT were transfected into PC12 cells by lipofectamin method, and intervened by application of curcumin (20 μmol/L) and 5-hydroxydecanoate (5-HD). Oligomer formation in the cultured cells was identified by Western blotting and Dot blotting. Cytotoxicity and apoptosis of the PC12 cells were measured by lactate dehydrogenase (LDH) and JC-1 assays. mitoKATP were identified by Western blotting and whole cell patch clamp.
  
  
  RESULTS
  Curcumin has significantly reduced the oligomer formation induced by overexpression or mutation of α-synuclein in the cultured cells. LDH has decreased by 36.3% and 23.5%, and red/green fluorescence ratio of JC-1 was increased respectively by 48.46% and 50.33% after application of curcumin (P<0.05). Protein expression of Kir6.2 has decreased and mitoKATP channel current has significantly increased (P<0.05).
  
  
  CONCLUSIONS
  Curcumin can inhibit α-synuclein gene overexpression or mutation induced α-synuclein oligomers formation. It may block apoptosis induced by wild-type overexpression or mutation of α-synuclein. By stabilizing mitochondrial membrane potential. Opening of mitoKATP channel may have been the initiating protective mechanism of apoptosis induced by wild-type overexpression or mutation of α-synuclein. Curcumin may antagonize above cytotoxicity through further opening the mitoKATP channel.
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P. R. China. ctxwyc@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Yidong
authors.initials: Y
authors.lastname: Deng
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liao
authors.forename: Jiannong
authors.initials: J
authors.lastname: Zhao
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wen
authors.forename: Guohu
authors.initials: G
authors.lastname: Weng
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D054086:KATP Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000187:drug effects; D016716:PC12 Cells; D010300:Parkinson Disease / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D051844:alpha-Synuclein / Q000235:genetics*
chemical_list: D054086:KATP Channels; D051844:alpha-Synuclein; D003474:Curcumin