title: Expression, purification, crystallization and X-ray crystallographic analysis of the periplasmic binding protein VatD from Vibrio vulnificus M2799.
abstract: Vibrio vulnificus is a halophilic marine microorganism which causes gastroenteritis and primary septicaemia in humans. An important factor that determines the survival of V. vulnificus in the human body is its ability to acquire iron. VatD is a periplasmic siderophore-binding protein from V. vulnificus M2799. The current study reports the expression, purification and crystallization of VatD. Crystals of both apo VatD and a VatD-desferrioxamine B-Fe(3+) (VatD-FOB) complex were obtained. The crystal of apo VatD belonged to space group P6422, while the crystal of the VatD-FOB complex belonged to space group P21. The difference in the two crystal forms could be caused by the binding of FOB to VatD.
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Miyano
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Igarashi
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawano
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Katsushiro
authors.initials: K
authors.lastname: Miyamoto
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuchiya
authors.affiliation: Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tomoo
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujibo
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D003676:Deferoxamine / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007501:Iron / Q000737:chemistry; D008969:Molecular Sequence Data; D019897:Periplasm / Q000737:chemistry* / Q000378:metabolism; D033902:Periplasmic Binding Proteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017262:Siderophores / Q000737:chemistry; D041261:Vibrio vulnificus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D033902:Periplasmic Binding Proteins; D011993:Recombinant Fusion Proteins; D017262:Siderophores; D007501:Iron; D003676:Deferoxamine
keywords: Vibrio vulnificus; desferrioxamine B; iron; periplasmic binding protein; siderophore