title: Manipulating the banana rhizosphere microbiome for biological control of Panama disease.
abstract: Panama disease caused by Fusarium oxysporum f. sp. cubense infection on banana is devastating banana plantations worldwide. Biological control has been proposed to suppress Panama disease, though the stability and survival of bio-control microorganisms in field setting is largely unknown. In order to develop a bio-control strategy for this disease, 16S rRNA gene sequencing was used to assess the microbial community of a disease-suppressive soil. Bacillus was identified as the dominant bacterial group in the suppressive soil. For this reason, B. amyloliquefaciens NJN-6 isolated from the suppressive soil was selected as a potential bio-control agent. A bioorganic fertilizer (BIO), formulated by combining this isolate with compost, was applied in nursery pots to assess the bio-control of Panama disease. Results showed that BIO significantly decreased disease incidence by 68.5%, resulting in a doubled yield. Moreover, bacterial community structure was significantly correlated to disease incidence and yield and Bacillus colonization was negatively correlated with pathogen abundance and disease incidence, but positively correlated to yield. In total, the application of BIO altered the rhizo-bacterial community by establishing beneficial strains that dominated the microbial community and decreased pathogen colonization in the banana rhizosphere, which plays an important role in the management of Panama disease.
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xue
authors.affiliation: College of Letters and Sciences, Faculty of Science and Mathematics, Arizona State University, Mesa, AZ, 85212, USA.
authors.forename: C Ryan
authors.initials: CR
authors.lastname: Penton
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Zongzhuan
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Ruifu
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Hainan Key Lab for Banana Planting, College of Agriculture, Hainan University, Haikou, Hainan, 570228, PR China.
authors.forename: Yunze
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D061046:Biological Control Agents / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D064307:Microbiota; D008969:Molecular Sequence Data; D028521:Musa / Q000254:growth & development / Q000382:microbiology*; D010176:Panama; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D061046:Biological Control Agents; D012336:RNA, Ribosomal, 16S