title: MLVF analysis of anginosus (milleri) group streptococci.
abstract: We developed a new method of typing for anginosus group streptococci (SAG). It is the first SAG-dedicated, PCR-based method, which allows to determine the relationship between strains. The method is based on the detection of tandem repeats among 9 genomic loci and is classified as multilocus variable number tandem repeats fingerprint (MLVF) type of analysis. Using the described method, it is possible to detect over half million MLVF patterns, which correlate with pulsed-field gel electrophoresis profiles. The other advantage of the method is relatively short time from "cell to data", low costs, and easy application for epidemiological and evolutionary studies.
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warszawa, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Obszańska
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warszawa, Poland.
authors.forename: Izabella
authors.initials: I
authors.lastname: Kern-Zdanowicz
authors.affiliation: Department of Molecular Microbiology, National Medicines Institute, Chełmska 30/34, 00-725, Warszawa, Poland. Electronic address: iza.sitkiewicz@gmail.com.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sitkiewicz
mesh_terms: D003365:Costs and Cost Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D013291:Streptococcus / Q000145:classification* / Q000235:genetics*; D013997:Time Factors
keywords: MLVF; Streptococcus anginosus; Typing