title: Protective Effects of Catechin against Monosodium Urate-Induced Inflammation through the Modulation of NLRP3 Inflammasome Activation.
abstract: Gouty inflammation results from the stimulation of monosodium urate (MSU). Interleukin-1β (IL-1β) secretion is the primary clinical manifestation of MSU attack, and MSU activates IL-1β through a nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome. This study investigated the protective effect and underlying mechanism of naturally occurring phenolic compounds on MSU-induced inflammation in vivo and in vitro. A screening of phenolic compounds revealed that gallic acid and catechin exhibited the most potent free radical scavenging activities. Subcutaneous injection of gallic acid or catechin significantly reduced MSU-induced IL-1β and IL-6 secretion in C57BL/6 mice. However, only catechin inhibited MSU-induced IL-1β secretion and NLRP3 inflammasome activation in MSU-challenged THP-1 cells. MSU-triggered mitochondrial reactive oxygen species (MtROS) production and intracellular calcium levels were significantly decreased by treatment with catechin in THP-1 cells. Catechin treatment also up-regulated Bcl-2 levels and restored MSU-induced mitochondrial transmembrane potential impairment. These results indicate that the protective effects of catechin on MSU-induced IL-1β secretion are associated with modulation of mitochondrial damage. It also suggests that catechin has the potential to protect gout attack by modulation of NLRP3 inflammasome activation.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Jhih-Jia
authors.initials: JJ
authors.lastname: Jhang
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Lu
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Ho
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Cheng
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.|Agricultural Biotechnology Center, National Chung Hsing University , Taichung 40227, Taiwan.
authors.forename: Gow-Chin
authors.initials: GC
authors.lastname: Yen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002392:Catechin / Q000494:pharmacology*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology; D005707:Gallic Acid / Q000494:pharmacology; D058847:Inflammasomes / Q000187:drug effects / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D018384:Oxidative Stress / Q000187:drug effects; D010538:Peritonitis / Q000139:chemically induced / Q000517:prevention & control; D020011:Protective Agents / Q000494:pharmacology; D014527:Uric Acid / Q000633:toxicity*
chemical_list: D002352:Carrier Proteins; D016166:Free Radical Scavengers; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D020011:Protective Agents; D014527:Uric Acid; D005707:Gallic Acid; D002392:Catechin; D002118:Calcium
keywords: IL-1β; NLRP3; catechin; inflammation; monosodium urate