title: Anti-allergic effect of α-cubebenoate isolated from Schisandra chinensis using in vivo and in vitro experiments.
abstract: OBJECTIVE
  In Oriental countries, the dried fruits of Schisandra chinensis are extensively used in traditional medicine to treat asthma, gonorrhea, and other diseases. Recently, α-cubebenoate was isolated as an anti-inflammatory component from Schisandra chinensis. In the present study, the authors examined the anti-allergic effect of α-cubebenoate using in vivo and in vitro experiments.
  
  
  METHODS
  α-Cubebenoate was isolated from an extract of Schisandra chinensis fruits. Antigen-induced degranulation and Ca(2+) mobilization were measured in RBL-2H3 mast cells. In addition, BALB/c mice were sensitized with ovalbumin and aluminum hydroxide, and then challenged with ovalbumin for three consecutive days. α-Cubebenoate (1mg/kg) was administered intraperitoneally 30min before each ovalbumin challenge.
  
  
  RESULTS
  In RBL-2H3 mast cells, α-cubebenoate inhibited antigen-induced degranulation and increase of intracellular Ca(2+) concentrations. In the ovalbumin-induced asthma model, α-cubebenoate suppressed bronchiolar structural changes induced by ovalbumin challenge. Furthermore, α-cubebenoate strongly inhibited accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. α-Cubebenoate also suppressed Th2 cytokines (IL-4 and IL-13) and TGF-β1 in lung tissues and in immune cells at the mRNA and protein levels.
  
  
  CONCLUSIONS
  α-Cubebenoate has an inhibitory effect on allergic inflammation and could be utilized as an agent for the treatment of asthma.
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Kyoung-Pil
authors.initials: KP
authors.lastname: Lee
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Saeromi
authors.initials: S
authors.lastname: Kang
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Ae-Yeon
authors.initials: AY
authors.lastname: Lee
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Hae-Young
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University, Mirynang-si, Gyeongsangnam 627-706, Republic of Korea.
authors.forename: Young-Whan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Department of Food Engineering, College of Natural Resources & Life Science, Pusan National University, Mirynang-si, Gyeongsangnam 627-706, Republic of Korea.
authors.forename: Young-Geun
authors.initials: YG
authors.lastname: Lee
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: imds@pusan.ac.kr.
authors.forename: Dong-Soon
authors.initials: DS
authors.lastname: Im
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002452:Cell Count; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005638:Fruit; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D032141:Schisandra; D045789:Sesquiterpenes, Guaiane / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D012333:RNA, Messenger; D045789:Sesquiterpenes, Guaiane; C000598129:alpha-cubebenoate; D010047:Ovalbumin
keywords: Anti-allergy; Asthma; Mast cells; Schisandra chinensis; α-cubebenoate