title: Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance.
abstract: Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.
authors.affiliation: Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana.|These authors contributed equally to this work.
authors.forename: Meg
authors.initials: M
authors.lastname: Trahey
authors.affiliation: Department of Medicinal Chemistry, University of Washington, Seattle Washington.|These authors contributed equally to this work.
authors.forename: Mavis Jiarong
authors.initials: MJ
authors.lastname: Li
authors.affiliation: Department of Medicinal Chemistry, University of Washington, Seattle Washington.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Kwon
authors.affiliation: Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana.
authors.forename: Erica L
authors.initials: EL
authors.lastname: Woodahl
authors.affiliation: Department of Medicinal Chemistry, University of Washington, Seattle Washington.
authors.forename: Wynton D
authors.initials: WD
authors.lastname: McClary
authors.affiliation: Department of Medicinal Chemistry, University of Washington, Seattle Washington.
authors.forename: William M
authors.initials: WM
authors.lastname: Atkins
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques; D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D008055:Lipids; D008565:Membrane Proteins
keywords: antibody-receptor binding; biosensor; lipid nanodisc; membrane protein; surface plasmon resonance binding analysis