title: Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries).
abstract: Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 Å resolution are reported.
authors.affiliation: School of Applied Science and Engineering, Federation University, Churchill, Victoria 3842, Australia.
authors.forename: Dhanasekaran
authors.initials: D
authors.lastname: Sakthivel
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Dene
authors.initials: D
authors.lastname: Littler
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: Shahine
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Sally
authors.initials: S
authors.lastname: Troy
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Johnson
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Jamie
authors.initials: J
authors.lastname: Rossjohn
authors.affiliation: School of Applied Science and Engineering, Federation University, Churchill, Victoria 3842, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Piedrafita
authors.affiliation: Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Melbourne, Victoria 3086, Australia.
authors.forename: Travis
authors.initials: T
authors.lastname: Beddoe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D037161:Galectins / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D034561:Sheep, Domestic
chemical_list: D037161:Galectins; D011993:Recombinant Fusion Proteins
keywords: galectin; glycan; livestock; parasite