title: Isolation of segmented filamentous bacteria from complex gut microbiota.
abstract: Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates.
authors.affiliation: University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MO.|MU Metagenomics Center, Columbia, MO.|MU Rat Resource and Research Center, Columbia, MO.|Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO.
authors.forename: Aaron C
authors.initials: AC
authors.lastname: Ericsson
authors.affiliation: University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MO.|Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO.
authors.forename: Giedre
authors.initials: G
authors.lastname: Turner
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO.
authors.forename: Lisa
authors.initials: L
authors.lastname: Montoya
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO.
authors.forename: Annie
authors.initials: A
authors.lastname: Wolfe
authors.affiliation: Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA.
authors.forename: Stacey
authors.initials: S
authors.lastname: Meeker
authors.affiliation: Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA.
authors.forename: Charlie
authors.initials: C
authors.lastname: Hsu
authors.affiliation: Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA.
authors.forename: Lillian
authors.initials: L
authors.lastname: Maggio-Price
authors.affiliation: University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MO.|MU Metagenomics Center, Columbia, MO.|MU Rat Resource and Research Center, Columbia, MO.|Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO.
authors.forename: Craig L
authors.initials: CL
authors.lastname: Franklin
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D051379:Mice
keywords: Candidatus Arthromitus; SFB; commensal; culture; microbiota; segmented filamentous bacteria