title: CellProfiler: Novel Automated Image Segmentation Procedure for Super-Resolution Microscopy.
abstract: BACKGROUND
  Super resolution (SR) microscopy enabled cell biologists to visualize subcellular details up to 20 nm in resolution. This breakthrough in spatial resolution made image analysis a challenging procedure. Direct and automated segmentation of SR images remains largely unsolved, especially when it comes to providing meaningful biological interpretations.
  
  
  RESULTS
  Here, we introduce a novel automated imaging analysis routine, based on Gaussian, followed by a segmentation procedure using CellProfiler software (www.cellprofiler.org). We tested this method and succeeded to segment individual nuclear pore complexes stained with gp210 and pan-FG proteins and captured by two-color STED microscopy. Test results confirmed accuracy and robustness of the method even in noisy STED images of gp210.
  
  
  CONCLUSIONS
  Our pipeline and novel segmentation procedure may benefit end-users of SR microscopy to analyze their images and extract biologically significant quantitative data about them in user-friendly and fully-automated settings.
authors.affiliation: Department of Pediatrics and Adolescent Medicine, University Medical Center, University of Göttingen, Robert-Koch Str.40 1.D3.644 Histology Lab, Göttingen, 37075 Germany.
authors.forename: Kareem
authors.initials: K
authors.lastname: Soliman