title: Identification of benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing.
abstract: DNA-based stable-isotope probing (DNA-SIP) was used in this study to investigate the uncultivated bacteria with benzo[a]pyrene (BaP) metabolism capacities in two Chinese forest soils (Mt. Maoer in Heilongjiang Province and Mt. Baicaowa in Hubei Province). We characterized three different phylotypes with responsibility for BaP degradation, none of which were previously reported as BaP-degrading microorganisms by SIP. In Mt. Maoer soil microcosms, the putative BaP degraders were classified as belonging to the genus Terrimonas (family Chitinophagaceae, order Sphingobacteriales), whereas Burkholderia spp. were the key BaP degraders in Mt. Baicaowa soils. The addition of metabolic salicylate significantly increased BaP degradation efficiency in Mt. Maoer soils, and the BaP-metabolizing bacteria shifted to the microorganisms in the family Oxalobacteraceae (genus unclassified). Meanwhile, salicylate addition did not change either BaP degradation or putative BaP degraders in Mt. Baicaowa. Polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) genes were amplified, sequenced, and quantified in the DNA-SIP (13)C heavy fraction to further confirm the BaP metabolism. By illuminating the microbial diversity and salicylate additive effects on BaP degradation across different soils, the results increased our understanding of BaP natural attenuation and provided a possible approach to enhance the bioremediation of BaP-contaminated soils.
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Mengke
authors.initials: M
authors.lastname: Song
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China clluo@gig.ac.cn.
authors.forename: Chunling
authors.initials: C
authors.lastname: Luo
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster, United Kingdom.
authors.forename: Dayi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Gan
authors.initials: G
authors.lastname: Zhang
mesh_terms: D041963:Bacteroidetes / Q000145:classification / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D019117:Burkholderia / Q000145:classification / Q000378:metabolism*; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics; D065928:Forests; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D042802:Oxalobacteraceae / Q000145:classification / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D001564:Benzo(a)pyrene; D049308:Dioxygenases