title: Physicochemical properties and antioxidant activities of polysaccharide from floral mushroom cultivated in Huangshan Mountain.
abstract: In this paper, a polysaccharide fraction (FMPS) was purified from the floral mushroom cultivated in Huangshan Mountain for the first time. Physicochemical properties and antioxidant activities of FMPS were investigated. FMPS had an average molecular weight of 7.2×10(5)Da and was composed of glucose. On the basis of FT-IR, NMR and methylation analysis, the repeating unit of FMPS was established as (1→3)-linked β-d-glucopyranosyl backbone with 1-linked β-d-glucopyranosyl branches substituted at O-6 position of (1→3)-linked β-d-glucopyranosyl residues. The advanced structure studies indicated that FMPS was a triple-helical polysaccharide. The main hydrodynamic radius (Rh) of FMPS was 23.4nm and it could form a stable system with water in 1.2×10(-2)g/mL solutions. In addition, FMPS exhibited high DPPH radical scavenging activities (79.46% at 5mg/mL) and hydroxyl radical scavenging activities (74.18% at 5mg/mL), as well as Fe(2+)chelating activities and ABTS radical scavenging activities to some extent.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: junhuiwang@hfut.edu.cn.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Xu
authors.affiliation: Center of Analysis and Measurement, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Jing-Cheng
authors.initials: JC
authors.lastname: Zhang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Han-Ju
authors.initials: HJ
authors.lastname: Sun
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Zha
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D055598:Chemical Phenomena; D002850:Chromatography, Gel; D003224:Congo Red; D017753:Ecosystem; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D003224:Congo Red
keywords: Antioxidant activities; Floral mushroom; Physicochemical properties; Polysaccharides