title: Knockdown of PFTK1 Inhibits the Migration of Glioma Cells.
abstract: The prognosis of glioma patients is generally poor, so it is urgent to find out the underlying molecular mechanisms. PFTK1 is a member of cyclin-dependent kinases (Cdks) family and has been reported to contribute to tumor migration and invasion. In this study, we aimed to explore the expression and function in human glioma. Western blot and immunohistochemistry were used to evaluate the expression of PFTK1. PFTK1 expression was higher in glioma tissues compared with normal brain tissues, and its level was associated with the WHO grade in Western blot analysis. The suppression of PFTK1 expression by RNA interference was shown to inhibit the migration of glioma cells. Knockdown of PFTK1 increases E-cadherin expression and decreases vimentin expression. These data show that PFTK1 may participate in the pathogenic process of glioma, suggesting that PFTK1 can become a potential therapeutic strategy for gastric cancer.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chengjin
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Shirong
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Xiubing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Ban
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, People's Republic of China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, People's Republic of China. yilugao2015@163.com.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, People's Republic of China. yilugao2015@163.com.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China. taotaont2015@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D014746:Vimentin; C515076:CDK14 protein, human; D018844:Cyclin-Dependent Kinases
keywords: E-cadherin; Glioma; Knockdown; Migration; PFTK1