title: Fine genetic mapping of the white immature fruit color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.).
abstract: The white immature fruit color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin color of immature fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature fruit color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature fruit color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ("9930" and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ("9930" draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Hanqiang
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Huanwen
authors.initials: H
authors.lastname: Meng
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Xinjing
authors.initials: X
authors.lastname: Liang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Jiao
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Shuxia
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. chengzh@nwsuaf.edu.cn.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003116:Color; D018553:Cucumis sativus / Q000201:enzymology / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005638:Fruit / Q000235:genetics*; D017343:Genes, Plant; D005808:Genes, Recessive; D056426:Genetic Loci; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics; D010641:Phenotype; D000069600:Plant Breeding; D020641:Polymorphism, Single Nucleotide
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D010544:Peroxidases