title: Identification of Cyclin A Binders with a Fluorescent Peptide Sensor.
abstract: A peptide sensor that integrates the 4-dimethylaminophthalimide (4-DMAP) fluorophore in a short cyclin A binding sequence displays a large fluorescence emission increase upon interacting with the cyclin A Binding Groove (CBG). Competitive displacement assays of this probe allow the straightforward identification of peptides that interact with the CBG, which could potentially block the recognition of CDK/cyclin A kinase substrates.
authors.affiliation: Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, C/ Jenaro de la Fuente s/n, Campus Vida, 15782, Santiago de Compostela, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Pazos
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
mesh_terms: D015761:4-Aminopyridine / Q000031:analogs & derivatives / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D001665:Binding Sites; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D019925:Cyclin A / Q000737:chemistry*; D018844:Cyclin-Dependent Kinases / Q000737:chemistry; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D005612:Freeze Drying; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000596:Amino Acids; D019925:Cyclin A; D005456:Fluorescent Dyes; D010455:Peptides; D015761:4-Aminopyridine; D018844:Cyclin-Dependent Kinases; C003885:4-dimethylaminopyridine