title: Evaluation of Biocompatibility of Root Canal Sealers on L929 Fibroblasts with Multiscan EX Spectrophotometer.
abstract: BACKGROUND
  The purpose of the current study was to estimate the biocompatibility of endodontic sealers with different bases on L929 mouse fibroblasts permanent cell line using Multiscan EX Spectrophotometer.
  
  
  METHODS
  Endodontics sealers used in this study were GuttaFlow (Roeko) silicone based sealer, AH plus (De Tray-DENTSPLY) epoxy resin based, Apexit (Vivadent) calcium hydroxide based and Endorez (Ultradent) methacrylate based sealer. Sealer were tested trough time, freshly mixed 24 h, 48h and 7 days after setting. Biocompatibility was determinate on permanent cell lines L929 mouse fibroblasts trough cytotoxicity using MTT assay. Level of absorption was measured with multi scan EX spectrophotometer on length 420-600 nm.
  
  
  RESULTS
  Sealer based on calcium hydroxide Apexit Plus, GuttaFlow silicone based sealer and AH plus epoxy resin based sealer, have shown a low cytotoxicity through the all periods of time on culture of L292 mouse fibroblasts. Methacrylate based sealer, Endorez showed moderate cytotoxicity when freshly mixed and after 7 days. After 24 hours the visibility of the cells was 74,0% and after 48 hours 65,1%. which is slightly cytotoxic.
  
  
  CONCLUSIONS
  According to results of this study there is a statistically significant difference among the groups p<0,05 for all the tested sealers. Apexit Plus, GuttaFlow and AH plus can be considered as biocompatibile. EndoREZ sealer which is based on methacrylate, after 7 days shows 50,1% of visible live cells which is considered as moderate cytotoxicity.
authors.affiliation: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors.forename: Alma
authors.initials: A
authors.lastname: Konjhodzic-Prcic
authors.affiliation: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors.forename: Selma
authors.initials: S
authors.lastname: Jakupovic
authors.affiliation: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors.forename: Lajla
authors.initials: L
authors.lastname: Hasic-Brankovic
authors.affiliation: Department of Dental Morphology with Dental Anthropology and Forensics Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors.forename: Amra
authors.initials: A
authors.lastname: Vukovic
keywords: biocompatibility; multiscan EX Spectrometer; root canal sealers