title: Improved Salinity Tolerance in Carrizo Citrange Rootstock through Overexpression of Glyoxalase System Genes.
abstract: Citrus plants are widely cultivated around the world and, however, are one of the most salt stress sensitive crops. To improve salinity tolerance, transgenic Carrizo citrange rootstocks that overexpress glyoxalase I and glyoxalase II genes were obtained and their salt stress tolerance was evaluated. Molecular analysis showed high expression for both glyoxalase genes (BjGlyI and PgGlyII) in 5H03 and 5H04 lines. Under control conditions, transgenic and wild type plants presented normal morphology. In salinity treatments, the transgenic plants showed less yellowing, marginal burn in lower leaves and showed less than 40% of leaf damage compared with wild type plants. The transgenic plants showed a significant increase in the dry weight of shoot but there are no differences in the root and complete plant dry weight. In addition, a higher accumulation of chlorine is observed in the roots in transgenic line 5H03 but in shoot it was lower. Also, the wild type plant accumulated around 20% more chlorine in the shoot compared to roots. These results suggest that heterologous expression of glyoxalase system genes could enhance salt stress tolerance in Carrizo citrange rootstock and could be a good biotechnological approach to improve the abiotic stress tolerance in woody plant species.
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Alameda 340, P.O. Box 114-D, 8331150 Santiago, Chile ; Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4560, 7820436 Santiago, Chile.
authors.forename: Ximena
authors.initials: X
authors.lastname: Alvarez-Gerding
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Alameda 340, P.O. Box 114-D, 8331150 Santiago, Chile.
authors.forename: Rowena
authors.initials: R
authors.lastname: Cortés-Bullemore
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Alameda 340, P.O. Box 114-D, 8331150 Santiago, Chile.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Medina
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Alameda 340, P.O. Box 114-D, 8331150 Santiago, Chile ; Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4560, 7820436 Santiago, Chile ; Instituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Boulevard Juan de Dios Bátiz Paredes No. 250, San Joachín, CP 81101, Guasave, SIN, Mexico.
authors.forename: Jesús L
authors.initials: JL
authors.lastname: Romero-Romero
authors.affiliation: Núcleo de Investigación en Producción Alimentaria, Facultad de Recursos Naturales, Escuela de Agronomía, Universidad Católica de Temuco, P.O. Box 56-D, Temuco, Chile.
authors.forename: Claudio
authors.initials: C
authors.lastname: Inostroza-Blancheteau
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Alameda 340, P.O. Box 114-D, 8331150 Santiago, Chile ; Laboratorio de Bioingeniería, Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibañez, Diagonal Las Torres 2640, Peñalolen, 7941169 Santiago, Chile ; Center for Applied Ecology and Sustainability (CAPES), Santiago, Chile.
authors.forename: Felipe
authors.initials: F
authors.lastname: Aquea
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Alameda 340, P.O. Box 114-D, 8331150 Santiago, Chile.
authors.forename: Patricio
authors.initials: P
authors.lastname: Arce-Johnson
mesh_terms: D002957:Citrus / Q000201:enzymology / Q000235:genetics; D007791:Lactoylglutathione Lyase / Q000096:biosynthesis / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D055049:Salt Tolerance; D013869:Thiolester Hydrolases / Q000096:biosynthesis / Q000235:genetics; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D007791:Lactoylglutathione Lyase; D010940:Plant Proteins; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase