title: Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide-Based Chemosensing Ensemble.
abstract: We described a new ensemble-approach-based chemosensor, NCH-Cu(2+), for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu(2+) ions over other metal ions, accompanied with a 92.2% fluorescence quenching. The decomplexation of NCH-Cu(2+) ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu(2+) towards His showed a good linearity with a detection of limit at 70 nm. Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH-Cu(2+) towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH-Cu(2+) was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, 114051, P.R. China. qtmeng@ustl.edu.cn.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, 114051, P.R. China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Jia
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, 114051, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, 114051, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney NSW, 2109, Australia. run.zhang@mq.edu.au.
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Renjie
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, 114051, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006639:Histidine / Q000032:analysis*; D006801:Humans; D007477:Ions / Q000737:chemistry; D018613:Microscopy, Confocal; D053644:Naphthalimides / Q000737:chemistry* / Q000633:toxicity; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D053644:Naphthalimides; D006639:Histidine; D003300:Copper
keywords: chemosensor; fluorescence imaging; histidine; lysosome; quantification