title: Expression profile of parkin isoforms in human gliomas.
abstract: Mutations of parkin gene are not restricted to familial forms of Parkinsonism but they also occur in a wide variety of malignancies including gliomas. Parkin over-expression reduces glioma cells proliferation and analysis of its expression is predictive for the survival outcome of patients with glioma. To date have been identified 21 parkin alternative splice variants. However, most of the studies have focused their attention exclusively on full-length protein. In the present study, the expression profile of parkin isoforms in different grades of astrocytomas was analyzed for the first time, in order to evaluate their involvement in this malignancy. Furthermore, to investigate their role in cellular processes, their expression in three glioblastoma cell lines was analyzed following treatment with the proteasome inhibitor MG132, or induction of mitophagy with CCCP, or after serum deprivation. Results suggested that H20, H1 and H5 isoforms are always expressed in tumors both in vivo and in vitro models. Therefore, these isoforms might be used as specific biomarkers to develop a prognostic tool for brain tumors.
authors.affiliation: Department of Biomedical Sciences and Biotechnology, Section of Anatomy and Histology, University of Catania, Catania, Italy.
authors.forename: Grazia
authors.initials: G
authors.lastname: Maugeri
authors.affiliation: Department of Biomedical Sciences and Biotechnology, Section of Anatomy and Histology, University of Catania, Catania, Italy.
authors.forename: Agata Grazia
authors.initials: AG
authors.lastname: D'Amico
authors.affiliation: G.F. Ingrassia Department, Anatomic Pathology, University of Catania, Catania, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Magro
authors.affiliation: G.F. Ingrassia Department, Anatomic Pathology, University of Catania, Catania, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Salvatorelli
authors.affiliation: Neurosurgery Department, Policlinico 'G. Rodolico' University Hospital, Catania, Italy.
authors.forename: Giuseppe M V
authors.initials: GM
authors.lastname: Barbagallo
authors.affiliation: Department of Biological, Geological and Environmental Sciences, Section of Animal Biology, University of Catania, Catania, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Saccone
authors.affiliation: Department of Biomedical Sciences and Biotechnology, Section of Pharmacology and Biochemistry, University of Catania, Catania, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Drago
authors.affiliation: Functional Genomics Center, Institute of Neurological Sciences, Italian National Research Council, Catania, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Cavallaro
authors.affiliation: Department of Biomedical Sciences and Biotechnology, Section of Anatomy and Histology, University of Catania, Catania, Italy.
authors.forename: Velia
authors.initials: V
authors.lastname: D'Agata
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000096:biosynthesis; D059467:Transcriptome; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein