title: Influence of gentamicin-coded PVDF suture material on the healing of intestinal anastomosis in a rat model.
abstract: OBJECTIVE
  Intestinal anastomosis is a fundamental procedure in general surgery and required to restore intestinal continuity following resection. The aim of this study was to evaluate whether a gentamicin-coated polyvinylidene fluoride (PVDF) suture material has beneficial effect on anastomotic healing.
  
  
  METHODS
  Ninety Sprague-Dawley rats were divided into three groups: a PVDF-suture group, a gentamicin-coated PVDF (GPVDF)-suture group and a control group using Maxon® (polyglycolid-co-trimethylene carbonate). For each animal, a colonic anastomosis was performed. Ten animals from each group were sacrificed on postoperative days 3, 5, and 14. Measurements of anastomotic bursting pressure were performed on days 3 and 5. At each time, collagen type I/III ratio, MMP 2 and MMP-9 expression and the proliferation index (Ki67) were analyzed.
  
  
  RESULTS
  In total, 90 animals underwent surgery without postoperative complications. Bursting strength in the GPVDF group was significantly elevated on day 5. Immunohistochemistry showed significant increase of the collagen type I/III ratio for PVDF and GPVDF on days 3 and 5. MMP2 was significantly increased for PVDF on days 3 and 5 and for GPVDF on day 5. The analysis of MMP9 revealed significant increase compared to control on day 3 and 5 (GPVDF) as well as on day 5 (PVDF). Staining for Ki67 revealed a significant elevation on postoperative day 3 for the PVDF and the GPVDF group.
  
  
  CONCLUSIONS
  The present data shows the feasibility of PVDF as suture material for colonic anastomosis and confirms the ability of gentamicin to increase the stability of colonic anastomosis when used as coating material.
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany. dominikschoeb@gmx.de.
authors.forename: Dominik S
authors.initials: DS
authors.lastname: Schoeb
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Christian D
authors.initials: CD
authors.lastname: Klink
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Lambertz
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Eickhoff
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Busch
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Tom F
authors.initials: TF
authors.lastname: Ulmer
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Ulf P
authors.initials: UP
authors.lastname: Neumann
authors.affiliation: Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Binnebösel
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005839:Gentamicins; D007422:Intestines / Q000601:surgery*; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D023421:Models, Animal; D011145:Polyvinyls; D011500:Protein Synthesis Inhibitors; D017207:Rats, Sprague-Dawley; D013537:Sutures; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D005465:Fluorocarbon Polymers
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D005839:Gentamicins; D019394:Ki-67 Antigen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D011145:Polyvinyls; D011500:Protein Synthesis Inhibitors; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: Bursting pressure; Collagen type I/III ratio; Gentamicin; Intestinal anastomosis; Polyvinylidene fluoride (PVDF); Suture material