title: Solid-state fermentation in multi-well plates to assess pretreatment efficiency of rot fungi on lignocellulose biomass.
abstract: The potential of fungal pretreatment to improve fermentable sugar yields from wheat straw or Miscanthus was investigated. We assessed 63 fungal strains including 53 white-rot and 10 brown-rot fungi belonging to the Basidiomycota phylum in an original 12 day small-scale solid-state fermentation (SSF) experiment using 24-well plates. This method offers the convenience of one-pot processing of samples from SSF to enzymatic hydrolysis. The comparison of the lignocellulolytic activity profiles of white-rot fungi and brown-rot fungi showed different behaviours. The hierarchical clustering according to glucose and reducing sugars released from each biomass after 72 h enzymatic hydrolysis splits the set of fungal strains into three groups: efficient, no-effect and detrimental-effect species. The efficient group contained 17 species belonging to seven white-rot genera and one brown-rot genus. The yield of sugar released increased significantly (max. 62%) compared with non-inoculated controls for both substrates.
authors.affiliation: INRA, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|Aix-Marseille Université, Polytech Marseille, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.
authors.forename: Simeng
authors.initials: S
authors.lastname: Zhou
authors.affiliation: INRA, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|Aix-Marseille Université, Polytech Marseille, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.
authors.forename: Sana
authors.initials: S
authors.lastname: Raouche
authors.affiliation: INRA, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|Aix-Marseille Université, Polytech Marseille, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.
authors.forename: Sacha
authors.initials: S
authors.lastname: Grisel
authors.affiliation: INRA, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|Aix-Marseille Université, Polytech Marseille, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|International Centre for Microbial Resources collection-Filamentous Fungi, CIRM-CF, F-13009, Marseille, France.
authors.forename: David
authors.initials: D
authors.lastname: Navarro
authors.affiliation: INRA, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|Aix-Marseille Université, Polytech Marseille, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Sigoillot
authors.affiliation: INRA, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.|Aix-Marseille Université, Polytech Marseille, UMR 1163 Biodiversity and Biotechnology of Fungi, F-13009, Marseille, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Herpoël-Gimbert
mesh_terms: D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis*; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D008031:Lignin