title: Computational study of remodeling in a nucleosomal array.
abstract: Chromatin remodeling complexes utilize the energy of ATP hydrolysis to change the packing state of chromatin, e.g. by catalysing the sliding of nucleosomes along DNA. Here we present simple models to describe experimental data of changes in DNA accessibility along a synthetic, repetitive array of nucleosomes during remodeling by the ACF enzyme or its isolated ATPase subunit, ISWI. We find substantial qualitative differences between the remodeling activities of ISWI and ACF. To understand better the observed behavior for the ACF remodeler, we study more microscopic models of nucleosomal arrays.
authors.affiliation: Instituut-Lorentz, Leiden University, P.O. Box 9506, 2300 RA, Leiden, The Netherlands.
authors.forename: Raoul D
authors.initials: RD
authors.lastname: Schram
authors.forename: Henrike
authors.initials: H
authors.lastname: Klinker
authors.forename: Peter B
authors.initials: PB
authors.lastname: Becker
authors.forename: Helmut
authors.initials: H
authors.lastname: Schiessel
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D042002:Chromatin Assembly and Disassembly; D003198:Computer Simulation; D004330:Drosophila / Q000737:chemistry / Q000235:genetics; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
chemical_list: C086468:ISWI protein; D009707:Nucleosomes; D014157:Transcription Factors; D000251:Adenosine Triphosphatases