title: Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil.
abstract: In this study, the essential oil (EO) constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%), myrcene (2.5%), germacrene B (1.4%), elemicine (1.1%) and carvacrol (0.5%). The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma) and MCF-7 (breast adenocarcinoma) human cancer cell lines (p < 0.05). Antimicrobial screening of S. intermedia EO demonstrated slight antibacterial and antifungal activities against Streptococcus mutants, S. salivarius, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and C. glabrata. Further preclinical studies are needed to assess the efficacy and safety of S. intermedia EO as a new promising anticancer agent.
authors.affiliation: Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol 61615-585, Iran. javad.sharifirad@gmail.com.|Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol 61615-585, Iran. javad.sharifirad@gmail.com.
authors.forename: Javad
authors.initials: J
authors.lastname: Sharifi-Rad
authors.affiliation: Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol 61663-335, Iran. mehdi_sharifirad@yahoo.com.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sharifi-Rad
authors.affiliation: Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran 15468-15514, Iran. m.hoseinialfatemi@gmail.com.
authors.forename: Seyedeh Mahsan
authors.initials: SM
authors.lastname: Hoseini-Alfatemi
authors.affiliation: Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, Milan 20133, Italy. marcello.iriti@unimi.it.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iriti
authors.affiliation: Department of Range and Watershed Management, Faculty of Natural Resources, University of Zabol, Zabol 98615-538, Iran. majid.sharifirad@gmail.com.
authors.forename: Majid
authors.initials: M
authors.lastname: Sharifi-Rad
authors.affiliation: Department of Chemistry, Faculty of Science, University of Zabol, Zabol 98615-538, Iran. marzieh.sharifirad@gmail.com.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Sharifi-Rad
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002175:Candida / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D000068378:Lamiales / Q000737:chemistry*; D061986:MCF-7 Cells; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D013729:Terpenes / Q000032:analysis
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D009822:Oils, Volatile; D013729:Terpenes
keywords: C/MS; breast adenocarcinoma; hepatocellular carcinoma; human cancer cells; oral pathogens