title: Rac1 and ROCK are implicated in the cell surface delivery of GLUT4 under the control of the insulin signal mimetic diDCP-LA-PE.
abstract: The phosphatidylethanolamine derivative 1,2-O-bis-[8-{2-(2-pentyl-cyclopropylmethyl)-cyclopropyl}-octanoyl]-sn-glycero-3-phosphatidylethanolamine (diDCP-LA-PE) promoted GLUT4 translocation to the cell surface in differentiated 3T3-L1-GLUT4myc adipocytes through a pathway along a phosphatidylinositol 3-kinase (PI3K)/3-phosphoinositide-dependent protein kinase-1 (PDK1)/Akt axis, that mimics insulin signaling. Moreover, diDCP-LA-PE-induced GLUT4 translocation was suppressed by inhibitors of the Rho GTPase Rac1 and Rho-associated coiled-coil-containing protein kinase (ROCK) or knocking-down Rac1 and ROCK1. The results of the present study show that Rac1 and ROCK are critical for regulation of GLUT4 trafficking by diDCP-LA-PE as well as insulin.
authors.affiliation: Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, Nishinomiya, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Tsuchiya
authors.affiliation: Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, Nishinomiya, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kanno
authors.affiliation: Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, Nishinomiya, Japan. Electronic address: tomoyuki@hyo-med.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nishizaki
mesh_terms: D064413:3-Phosphoinositide-Dependent Protein Kinases / Q000502:physiology; D017667:Adipocytes / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D006801:Humans; D007328:Insulin; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D058539:Phosphatidylinositol 3-Kinase / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects*; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D020830:rac1 GTP-Binding Protein / Q000502:physiology*; D054460:rho-Associated Kinases / Q000502:physiology*
chemical_list: C000607905:1,2-O-bis(8-(2-(2-pentylcyclopropylmethyl)cyclopropyl)octanoyl)-sn-glycero-3-phosphatidylethanolamine; D051275:Glucose Transporter Type 4; D007328:Insulin; D010714:Phosphatidylethanolamines; C107953:RAC1 protein, human; C495692:SLC2A4 protein, human; D058539:Phosphatidylinositol 3-Kinase; D064413:3-Phosphoinositide-Dependent Protein Kinases; C578562:PDPK1 protein, human; D051057:Proto-Oncogene Proteins c-akt; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases; D020830:rac1 GTP-Binding Protein
keywords: GLUT4 trafficking; ROCK; Rac1; diDCP-LA-PE