title: Microwave-assisted extraction and purification of chlorogenic acid from by-products of Eucommia Ulmoides Oliver and its potential anti-tumor activity.
abstract: An efficient method for the rapid extraction, separation and purification of chlorogenic acid (CGA) from by-products of Eucommia Ulmoides Oliver (E. ulmoides) by microwave-assisted extraction (MAE) coupled with high-speed counter-current chromatography (HSCCC) was developed. The optimal MAE parameters were evaluated by response surface methodology (RSM), and they were extraction time of 12 min, microwave power of 420 W, ethanol concentration of 75 %, solvent/sample ratio of 30:1 (mL/g), yield of CGA reached 3.59 %. The crude extract was separated and purified directly by HSCCC using ethyl acetate-butyl alcohol-water (3:1:4, v/v) as the two-phase solvent system. The 14.5 mg of CGA with the purity of 98.7 % was obtained in one-step separation from 400 mg of crude extract. The chemical structure of CGA was verified with IR, ESI-MS analysis. Meanwhile, the purified CGA extract was evaluated by MTT assay and results indicate that CGA extract exhibited potential anti-tumor activity for AGS gastric cancer cell.
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014 China.
authors.forename: P
authors.initials: P
authors.lastname: Shao
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014 China.
authors.forename: J F
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014 China.
authors.forename: X X
authors.initials: XX
authors.lastname: Chen
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014 China.
authors.forename: P L
authors.initials: PL
authors.lastname: Sun
keywords: Chlorogenic acid; Eucommia ulmoides Oliver; High-speed counter-current chromatography; Microwave-assisted; Response surface methodology