title: Silver nanoparticles anchored on nitrogen-doped graphene as a novel electrochemical biosensing platform with enhanced sensitivity for aptamer-based pesticide assay.
abstract: Silver nanoparticles (NPs) decorated nitrogen doped graphene (NG) nanocomposites were prepared through a one-step thermal-treatment route using arginine as the nitrogen source. By integrating the excellent electrical properties and large surface area of Ag NPs and NG, the obtained Ag/NG nanocomposites show more effective electron transfer and high loading capacity than Ag-graphene and pure NG. In the presence of a target, the stronger interaction between the aptamer and the target promotes the formation of a target-aptamer complex on the electrode surface which blocks the electron transfer. Based on this sensing mechanism, a novel and highly sensitive biosensing platform by the use of Ag/NG as enhancing materials is demonstrated for detection of the model target, acetamiprid. The presented aptasensor exhibited a wide linear response for acetamiprid in the range of 1 × 10(-13) M to 5 × 10(-9) M with a low detection limit of 3.3 × 10(-14) M (S/N = 3). Moreover, this electrochemical aptasensor avoided complicated labeling procedures and showed magnificent sensitivity, high selectivity and low cost, which made it not only convenient but also time-saving and applicable. Furthermore, the proposed design may offer a promising way to develop a new electrochemical aptasensor for sensitive and specific detection of a wide spectrum of analytes in food, medical and environmental fields.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, P.R. China.
authors.forename: Ding
authors.initials: D
authors.lastname: Jiang
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Du
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Liming
authors.initials: L
authors.lastname: Dai
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D000073943:Neonicotinoids; D009584:Nitrogen / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011725:Pyridines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012834:Silver / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D000073943:Neonicotinoids; D010575:Pesticides; D011725:Pyridines; D012834:Silver; C464485:acetamiprid; D006108:Graphite; D009584:Nitrogen