title: Highly sensitive electrochemical immunosensor for the detection of alpha fetoprotein based on PdNi nanoparticles and N-doped graphene nanoribbons.
abstract: An ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of alpha fetoprotein (AFP). The β-cyclodextrins functionalized graphene sheets (CD-GS) were used as the sensing matrix for immobilizing adamantine-1-carboxylic acid functionalized primary anti-AFP (ADA-Ab1) and enhanced the electron transfer. PdNi alloy nanoparticles decorated N-doped graphene nanoribbons (PdNi/N-GNRs) were used as labels of secondary anti-AFP (Ab2), and PdNi alloy nanoparticles (PdNi NPs) exhibited high catalytic performance towards the reduction of H2O2. Meanwhile, with good dispersion, large specific surface area and good catalytic performance, N-doped graphene nanoribbons (N-GNRs) significantly amplified the electrochemical signal. Under the optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.0001-16 ng/mL with a low detection limit of 0.03 pg/mL. Additionally, the proposed immunosensor showed high specificity, good reproducibility and long-term stability, which have promising application in bioassay analysis.
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China. Electronic address: dubin61@gmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D000509:alpha-Fetoproteins; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Alpha fetoprotein; Electrochemical immunosensor; N-doped graphene nanoribbons.; PdNi; Sandwich-type