title: Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs.
abstract: Enterotoxigenic Escherichia coli (ETEC) causes severe diarrhea in both neonatal and weaned pigs. Because the cholera toxin B subunit (CTB) has a high level of amino acid identity to the ETEC heat-labile toxin (LT) B-subunit (LTB), we selected MucoRice-CTB as a vaccine candidate against ETEC-induced pig diarrhea. When pregnant sows were orally immunized with MucoRice-CTB, increased amounts of antigen-specific IgG and IgA were produced in their sera. CTB-specific IgG was secreted in the colostrum and transferred passively to the sera of suckling piglets. IgA antibodies in the colostrum and milk remained high with a booster dose after farrowing. Additionally, when weaned minipigs were orally immunized with MucoRice-CTB, production of CTB-specific intestinal SIgA, as well as systemic IgG and IgA, was induced. To evaluate the cross-protective effect of MucoRice-CTB against ETEC diarrhea, intestinal loop assay with ETEC was conducted. The fluid volume accumulated in the loops of minipigs immunized with MucoRice-CTB was significantly lower than that in control minipigs, indicating that MucoRice-CTB-induced cross-reactive immunity could protect weaned pigs from diarrhea caused by ETEC. MucoRice-CTB could be a candidate oral vaccine for inducing both passive and active immunity to protect both suckling and weaned piglets from ETEC diarrhea.
authors.affiliation: Research Department, Nippon Institute for Biological Science, Japan; Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Takeyama
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Japan. Electronic address: yukiy@ims.u-tokyo.ac.jp.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Yuki
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Japan; Department of Pediatrics, Osaka City University Graduate School of Medicine, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tokuhara
authors.affiliation: Research Department, Nippon Institute for Biological Science, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Oroku
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Mejima
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Kurokawa
authors.affiliation: Rice Physiology Research Team, National Agriculture Research Center, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: Research Department, Nippon Institute for Biological Science, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kodama
authors.affiliation: Research Department, Nippon Institute for Biological Science, Japan; Nisseiken Co. Ltd., Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Nagai
authors.affiliation: Research Department, Nippon Institute for Biological Science, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Ueda
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Japan; International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D003126:Colostrum / Q000276:immunology; D003967:Diarrhea / Q000517:prevention & control / Q000662:veterinary*; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D018928:Immunity, Mucosal; D007116:Immunization, Passive; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008892:Milk / Q000276:immunology; D012275:Oryza / Q000235:genetics*; D011247:Pregnancy; D044967:Serum / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014611:Vaccination; D022462:Vaccines, Edible / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D022361:Escherichia coli Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022462:Vaccines, Edible
keywords: Mucosal immunity; Oral vaccine; Pig ETEC; Plant vaccine