title: Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway.
abstract: Lactoferrin (LF) is one of the most abundant proteins found in milk, and it has been reported to have anti-inflammatory properties. However, the anti-inflammatory effects of LF on lipopolysaccharide (LPS)-induced endometritis and the underlying molecular mechanisms remain to be elucidated. In this study, we evaluated the effects of LF on LPS-induced endometritis in mice. The endometritis model was established by the perfusion of mice with LPS. LF was administered by intraperitoneal injection 1h before and 12h after LPS induction. Our results demonstrated that LF significantly attenuated the histopathological changes in the uterus, reduced the activity of myeloperoxidase (MPO) and the levels of nitric oxide (NO), and inhibited the activation of NF-κB and the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a dose-dependent manner. The results suggest that LF has an anti-inflammatory effect on LPS-induced endometritis in mice. Therefore, LF may be a potential therapeutic agent for the treatment of endometritis.
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Weishi
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Kaiqiang
authors.initials: K
authors.lastname: Fu
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Jifang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Huatao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
authors.forename: Wenru
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China. Electronic address: rfcao@126.com.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Cao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004716:Endometritis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D004731:Endotoxins / Q000494:pharmacology*; D005260:Female; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D007781:Lactoferrin / Q000008:administration & dosage / Q000627:therapeutic use*; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D004731:Endotoxins; D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C044101:endotoxin, Escherichia coli; D007781:Lactoferrin
keywords: Endometritis; Inflammation; Lactoferrin; Lipopolysaccharide; NF-κB