title: Pro-apoptotic and Growth-inhibitory Effect of IFN-β-Overexpressing Canine Adipose Tissue-derived Mesenchymal Stem Cells Against Melanoma Cells.
abstract: BACKGROUND
  Canine melanoma is the most common type of tumor in dogs. We investigated the effects of canine interferon-beta (cIFN-β)-overexpressing adipose tissue-derived mesenchymal stem cells (cATMSCs) on apoptosis and proliferation of canine melanoma cells.
  
  
  METHODS
  Expression of IFN-β in cATMSCs was confirmed using reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assays. Flow cytometry was performed for cell-cycle analysis and apoptotic cell quantification of LMeC (melanoma) cells. Protein expression of cyclin D1, procaspase-3, activated caspase-3, and Bcl-2 homologous antagonist killer (Bak) was evaluated by western blot analysis.
  
  
  RESULTS
  Decreased proportions of cells in S- and G0/G1 phases were observed in parallel with decreased cyclin D1 expression in LMeC cells treated with cIFN-β-cATMSC-conditioned media. Protein expression of active forms of caspase 3 and Bak increased in response to treatment with cIFN-β-cATMSC-conditioned media.
  
  
  CONCLUSIONS
  IFN-β overexpression by cATMSCs was associated with pro-apoptotic and growth-inhibitory effects on canine melanoma cells. The antitumor effects of these cells have therapeutic potential for the treatment of canine melanoma.
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sei-Myoung
authors.initials: SM
authors.lastname: Han
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Chong-Woo
authors.initials: CW
authors.lastname: Park
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jin-Ok
authors.initials: JO
authors.lastname: Ahn
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Park
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Woo-Sung
authors.initials: WS
authors.lastname: Jung
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Kyoung-Won
authors.initials: KW
authors.lastname: Seo
authors.affiliation: Biostar Stem Cell Research Center, K-STEMCELL Co. Ltd., Seoul, Republic of Korea.
authors.forename: Jeong-Chan
authors.initials: JC
authors.lastname: Ra
authors.affiliation: Biostar Stem Cell Research Center, K-STEMCELL Co. Ltd., Seoul, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Kang
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea hyyoun@snu.ac.kr.
authors.forename: Hee-Woo
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea hyyoun@snu.ac.kr.
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Youn
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004285:Dogs; D004789:Enzyme Activation / Q000187:drug effects; D016899:Interferon-beta / Q000378:metabolism*; D008545:Melanoma / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D050998:bcl-2 Homologous Antagonist-Killer Protein; D016899:Interferon-beta; D053148:Caspase 3
keywords: Melanoma; apoptosis; cell cycle; interferon β; mesenchymal stem cells