title: Genetic diversity of human rhinoviruses in Cambodia during a three-year period reveals novel genetic types.
abstract: Acute respiratory viral infections are a major cause of morbidity during early childhood in developing countries. Human rhinoviruses are the most frequent cause of upper respiratory tract infections in humans, which can range in severity from asymptomatic to clinically severe disease. In this study we collected 4170 nasopharyngeal swabs from patients hospitalised with influenza-like illness in two Cambodian provincial hospitals between 2007 and 2010. Samples were screened for 18 respiratory viruses using 5 multiplex PCRs. A total of 11.2% of samples tested positive for human rhinoviruses (HRV). VP4/2 and VP1 regions were amplified and sequenced to study the distribution of rhinoviruses genotypes and species in Cambodia during this three-year period. Five novel genotypes, 2 species A, 2 species B and 1 species C were identified based on VP1 sequences. Co-infections with other viruses were demonstrated.
authors.affiliation: Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia.
authors.forename: Monica
authors.initials: M
authors.lastname: Naughtin
authors.affiliation: Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia.
authors.forename: Rith
authors.initials: R
authors.lastname: Sareth
authors.affiliation: Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia.
authors.forename: Anne-Charlotte
authors.initials: AC
authors.lastname: Sentilhes
authors.affiliation: Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia.
authors.forename: Sirenda
authors.initials: S
authors.lastname: Vong
authors.affiliation: Institut Pasteur, Biologie des Virus Entériques, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France; INSERM U994, Institut National de Santé et de La Recherche Médicale, Paris, France.
authors.forename: Marie-Line
authors.initials: ML
authors.lastname: Joffret
authors.affiliation: CEMIPAI, CPBS, UMR 5236, CNRS-UM1-UM2, 1919 route de Mende, 34293 Montpellier Cedex 5, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Cornillot
authors.affiliation: Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia.
authors.forename: Vincent
authors.initials: V
authors.lastname: Deubel
authors.affiliation: Institut Pasteur, Biologie des Virus Entériques, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France; INSERM U994, Institut National de Santé et de La Recherche Médicale, Paris, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Delpeyroux
authors.affiliation: CEMIPAI, CPBS, UMR 5236, CNRS-UM1-UM2, 1919 route de Mende, 34293 Montpellier Cedex 5, France; Cirad, UMR 17, Cirad-Ird, TA-A17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. Electronic address: roger.frutos@univ-montp2.fr.
authors.forename: Roger
authors.initials: R
authors.lastname: Frutos
authors.affiliation: Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia. Electronic address: buchyphilippe@hotmail.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
mesh_terms: D002160:Cambodia; D060085:Coinfection / Q000821:virology; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009305:Nasopharynx / Q000821:virology; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000821:virology*; D012229:Rhinovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017423:Sequence Analysis, RNA
keywords: Cambodia; Genetic diversity; Human rhinoviruses; Picornavirus