title: Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background.
abstract: The use of crop wild relative species to improve major crops performance is well established. Hordeum chilense has a high potential as a genetic donor to increase the carotenoid content of wheat. Crosses between the 7Hch H. chilense substitution lines in wheat and the wheat pairing homoeologous1b (ph1b) mutant allowed the development of wheat-H. chilense translocation lines for both 7Hchα and 7Hchβ chromosome arms in the wheat background. These translocation lines were characterized by in situ hybridization and using molecular markers. In addition, reverse phase chromatography (HPLC) analysis was carried out to evaluate the carotenoid content and both 7Hchα∙7AL and 7AS∙7Hchβ disomic translocation lines. The carotenoid content in 7Hchα∙7AL and 7AS∙7Hchβ disomic translocation lines was higher than the wheat-7Hch addition line and double amount of carotenoids than the wheat itself. A proteomic analysis confirmed that the presence of chromosome 7Hch introgressions in wheat scarcely altered the proteomic profile of the wheat flour. The Psy1 (Phytoene Synthase1) gene, which is the first committed step in the carotenoid biosynthetic pathway, was also cytogenetically mapped on the 7Hchα chromosome arm. These new wheat-H. chilense translocation lines can be used as a powerful tool in wheat breeding programs to enrich the diet in bioactive compounds.
authors.affiliation: Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apartado, Córdoba, Spain.
authors.forename: María-Dolores
authors.initials: MD
authors.lastname: Rey
authors.affiliation: Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apartado, Córdoba, Spain.
authors.forename: María-Carmen
authors.initials: MC
authors.lastname: Calderón
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Paterna, Valencia, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Rodrigo
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Paterna, Valencia, Spain.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Zacarías
authors.affiliation: Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apartado, Córdoba, Spain.
authors.forename: Enriqueta
authors.initials: E
authors.lastname: Alós
authors.affiliation: Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apartado, Córdoba, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Prieto
mesh_terms: D001939:Bread; D002874:Chromosome Mapping; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics*; D000069600:Plant Breeding; D040901:Proteomics; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*