title: Pseudouridine Chemical Labeling and Profiling.
abstract: Pseudouridine (Ψ) is the most abundant posttranscriptional RNA modification; yet little is known about its prevalence and function in messenger RNA, mostly due to the challenges in the transcriptome-wide detection of Ψ. Here, we report CeU-Seq-a selective chemical labeling and pull-down method for the comprehensive analysis of transcriptome-wide pseudouridylation; this sequencing method will hopefully pave the way for functional studies of Ψ-mediated biological regulation in the future.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China; Department of Chemical Biology and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, China. Electronic address: chengqi.yi@pku.edu.cn.
authors.forename: Chengqi
authors.initials: C
authors.lastname: Yi
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D011560:Pseudouridine / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D059467:Transcriptome / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011560:Pseudouridine
keywords: Chemical pull down; Pseudouridine; Selective labeling; Single-base resolution; Transcriptome-wide profiling; Whole-genome sequencing; mRNA modification