title: Application of the multifactor dimensionality reduction method in evaluation of the roles of multiple genes/enzymes in multidrug-resistant acquisition in Pseudomonas aeruginosa strains.
abstract: Multidrug-resistant Pseudomonas aeruginosa (MDRPA) infections are major threats to healthcare-associated infection control and the intrinsic molecular mechanisms of MDRPA are also unclear. We examined 348 isolates of P. aeruginosa, including 188 MDRPA and 160 non-MDRPA, obtained from five tertiary-care hospitals in Guangzhou, China. Significant correlations were found between gene/enzyme carriage and increased rates of antimicrobial resistance (P < 0·01). gyrA mutation, OprD loss and metallo-β-lactamase (MBL) presence were identified as crucial molecular risk factors for MDRPA acquisition by a combination of univariate logistic regression and a multifactor dimensionality reduction approach. The MDRPA rate was also elevated with the increase in positive numbers of those three determinants (P < 0·001). Thus, gyrA mutation, OprD loss and MBL presence may serve as predictors for early screening of MDRPA infections in clinical settings.
authors.affiliation: Department of Epidemiology and Health Statistics,Guangdong Pharmaceutical University,Guangzhou,China.
authors.forename: Z
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of Epidemiology and Health Statistics,Guangdong Pharmaceutical University,Guangzhou,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Environmental and School Health,Shajing Health Inspection Institute,Shenzhen,China.
authors.forename: J
authors.initials: J
authors.lastname: Bi
authors.affiliation: Department of Epidemic Prevention,Luogang Centers for Disease Control and Prevention,Guangzhou,China.
authors.forename: C
authors.initials: C
authors.lastname: Xie
authors.affiliation: Division of Infectious Diseases,The People's Hospital of Meizhou,Meizhou,China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: Division of Environmental Health,Public Health Laboratory Center,Guangdong Pharmaceutical University,Guangzhou,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Health Statistics,Guangdong Pharmaceutical University,Guangzhou,China.
authors.forename: X
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Epidemiology and Health Statistics,Guangdong Pharmaceutical University,Guangzhou,China.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002681:China; D024901:Drug Resistance, Multiple, Bacterial; D008826:Microbial Sensitivity Tests; D058011:Multifactor Dimensionality Reduction; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D062606:Tertiary Care Centers
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins
keywords: Genes/enzymes; Pseudomonas aeruginosa; multidrug-resistant; multifactor dimensionality reduction