title: New circulating biomarkers for predicting cardiovascular death in healthy population.
abstract: There is interest to analyse newer biomarkers to identify healthy individuals at risk to develop cardiovascular disease (CVD) incidents and death. To determine in healthy individuals new circulating protein biomarkers, whose systemic levels may be associated with the risk of future development of CVD incidents and death. The study was performed in 82 individuals from the Malmö Diet and Cancer study cohort, free from CVD of whom 41 developed CVD and 41 did not. Plasma proteins related to inflammation and thrombo-coagulating processes were analysed. α1-antitrypsin isotype 3 plasma levels were significantly higher while apolipoprotein J plasma levels were lower in participants that developed CVD incidents than those that did not develop acute cardiovascular episode. Of 82 participants, 17 died by CVD causes. There were proteins whose expression in plasma was significantly higher in participants suffering CVD death as compared with those that did not die by CVD. These proteins included: fibrinogen β-chain isotypes 1 and 3, fibrinogen-γ-chain isotype 2, vitamin D-binding protein isotypes 1, 2 and 3, α1-antitrypsin isotypes 3 and 6, haptoglobin isotypes 3,4,5 and 5, haemopexin isotypes 1 and 2, and Rho/Rac guanine nucleotide exchange factor 2. Moreover, apolipoprotein J plasma levels were found lower in participants that died by cardiovascular cause. Association between plasma levels of proteins and CVD death was independent of age, gender, conventional risk factors and plasma C-reactive protein levels. Several protein plasma levels and protein isotypes related to inflammation and thrombo-coagulating phenomena were independently associated with the risk of future CVD death.
authors.affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Melander
authors.affiliation: Instituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Modrego
authors.affiliation: Instituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Zamorano-León
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, Universidad Complutense, Madrid, Spain.
authors.forename: Juana M
authors.initials: JM
authors.lastname: Santos-Sancho
authors.affiliation: Instituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.|Department of Physiology, School of Medicine, Universidad Complutense, Madrid, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Lahera
authors.affiliation: Instituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.|Department of Medicine, School of Medicine, Universidad Complutense, Madrid, Spain.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: López-Farré
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood* / Q000401:mortality*; D016022:Case-Control Studies; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005500:Follow-Up Studies; D006262:Health; D006801:Humans; D008297:Male; D008875:Middle Aged; D014809:Vitamin D-Binding Protein / Q000097:blood
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D014809:Vitamin D-Binding Protein; C027539:fibrinopeptides gamma; D005340:Fibrinogen
keywords: cardiovascular death; plasma biomarkers; proteomics; risk factors