title: Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions.
abstract: Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research.
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Bastianina
authors.initials: B
authors.lastname: Scanu
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Forteschi
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy; Laboratory of Cell Signaling and Redox Biology, University of Sassari, Sassari, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Giordo
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy; Laboratory of Cell Signaling and Redox Biology, University of Sassari, Sassari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Cossu
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy; Laboratory of Cell Signaling and Redox Biology, University of Sassari, Sassari, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Posadino
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy; Quality Control Unit, Hospital University of Sassari (AOU), Sassari, Italy.
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy; Laboratory of Cell Signaling and Redox Biology, University of Sassari, Sassari, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Pintus
mesh_terms: D002392:Catechin / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D006801:Humans; D012709:Serum Albumin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013662:Tea / Q000737:chemistry*
chemical_list: D012709:Serum Albumin; D013662:Tea; D002392:Catechin