title: Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells.
abstract: Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors.
authors.affiliation: Department of Transgenic Animal Sciences, Stem Cells Technology Research Center, Tehran, Iran.
authors.forename: Tarlan
authors.initials: T
authors.lastname: Eslami-Arshaghi
authors.affiliation: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: M.Salehi@sbmu.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: Soleim_M@modares.ac.ir.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mazaher
authors.initials: M
authors.lastname: Gholipourmalekabadi
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Urogenital Stem Cell Research Center (UGSCRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Mossahebi-Mohammadi
authors.affiliation: Department of Stem Cell Biology, Stem Cells Technology Research Center, Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Department of Transgenic Animal Sciences, Stem Cells Technology Research Center, Tehran, Iran.
authors.forename: Hoda
authors.initials: H
authors.lastname: Rajabi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D005434:Flow Cytometry; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D051379:Mice; D053652:Nuclear Transfer Techniques
chemical_list: D015703:Antigens, CD
keywords: Growth factors; Nuclear transfer embryonic stem cells; Somatic cell nuclear transfer; T and B lymphocytes