title: Effect of multi-stage inoculation on the bacterial and fungal community structure during organic municipal solid wastes composting.
abstract: In this study, PCR-DGGE method was applied to investigate the impact of multi-stage inoculation treatment on the community composition of bacterial and fungal during municipal solid wastes (MSW) composting process. The results showed that the high temperature period was extended by the multi-stage inoculation treatment, 1day longer than initial-stage inoculation treatment, and 5days longer than non-inoculation treatment. The temperature of the secondary fermentation increased to 51°C with multi-stage inoculation treatment. The multi-stage inoculation method improved the community diversity of bacteria and fungi that the diversity indexes reached the maximum on the 17days and 20days respectively, avoided the competition between inoculations and indigenous microbes, and enhanced the growth of dominant microorganisms. The DNA sequence indicated that various kinds of uncultured microorganisms with determined ratios were detected, which were dominant microbes during the whole fermentation process. These findings call for further researches of compost microbial cultivation technology.
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; Lan Zhou Jiao Tong University, Lanzhou 730070, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Beidou
authors.initials: B
authors.lastname: Xi
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: He
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: dangling819@126.com.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Dang
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Tianxue
authors.initials: T
authors.lastname: Yang
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Li
authors.affiliation: Energy Saving & Environmental Protection & Occupational Safety and Health Research, China Academy of Railway Sciences, 100081, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Innovation Base of Groundwater and Environmental Systems Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
mesh_terms: D001419:Bacteria / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D005658:Fungi / Q000235:genetics; D006358:Hot Temperature; D059013:Microbial Consortia; D016133:Polymerase Chain Reaction; D012037:Refuse Disposal / Q000379:methods*; D012987:Soil; D012988:Soil Microbiology; D062611:Solid Waste
chemical_list: D012987:Soil; D062611:Solid Waste
keywords: Composting; Microbial community structure; Multi-inoculation; PCR-DGGE