title: Hyperbranched lysine-arginine copolymer for gene delivery.
abstract: Based on the reactivity of amine groups and carboxyl groups of L-lysine and L-arginine, thermal polymerization of these two natural amino acids results in hyperbranched lysine-arginine copolymers (P-lys-argX, where X refers to the relevant molar ratio of arginine to lysine). Hyperbranched polylysine (P-lys) and two derivatives (P-lys-arg0.10 and P-lys-arg0.20) have been prepared. The arginine-rich hyperbranched polymers can interact with plasmid DNA to form nano-sized particles. The polyplexes were physicochemically analyzed by agarose gel electrophoresis, dynamic light scattering, and zeta potential measurements. Furthermore, their transfection efficiency was assessed, employing COS-7, 293T, and HeLa cell lines. It was found that P-lys showed poorly in its ability of condensation with DNA and transfection efficiency. On the other hand, arginine-rich products resulted to significant enhancement of its transfection efficiency, which is dependent on the content of arginine in the polymers, and the cell line used. P-lys-arg0.20 exhibited better transfection efficiency under all the condition studied. Besides, P-lys-arg0.20 showed lower toxicity in COS-7 cells.
authors.affiliation: a College of Chemistry and Chemical Engineering , Xinyang Normal University , Xinyang 464000 , P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Peng
authors.affiliation: a College of Chemistry and Chemical Engineering , Xinyang Normal University , Xinyang 464000 , P. R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: a College of Chemistry and Chemical Engineering , Xinyang Normal University , Xinyang 464000 , P. R. China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yu
authors.affiliation: b Chemistry Department , College of Arts and Sciences, Drexel University , Philadelphia , PA 19104 , USA.
authors.forename: Lee
authors.initials: L
authors.lastname: Hoffman
authors.affiliation: a College of Chemistry and Chemical Engineering , Xinyang Normal University , Xinyang 464000 , P. R. China.
authors.forename: Xingkun
authors.initials: X
authors.lastname: Yang
mesh_terms: D065567:Absorption, Physiological; D000818:Animals; D001120:Arginine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry; D019556:COS Cells; D002522:Chlorocebus aethiops; D004274:DNA, Recombinant / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry* / Q000378:metabolism; D018014:Gene Transfer Techniques / Q000009:adverse effects; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D010316:Particle Size; D011994:Recombinant Proteins / Q000378:metabolism; D013499:Surface Properties
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D001120:Arginine; D008239:Lysine
keywords: arginine; gene delivery; hyperbranched; lysine