title: Biological Effects of the Herbal Plant-Derived Phytoestrogen Bavachin in Primary Rat Chondrocytes.
abstract: The aim of this study was to examine the anabolic and anticatabolic functions of bavachin in primary rat chondrocytes. With bavachin treatment, chondrocytes survived for 21 d without cell proliferation, and the proteoglycan content and extracellular matrix increased. Short-term monolayer culture of chondrocytes showed that gene induction of both aggrecan and collagen type II, major extracellular matrix components, was significantly upregulated by bavachin. The expression and activities of cartilage-degrading enzymes such as matrix metalloproteinases and a disintegrin and metalloproteinase with thrombospondin motifs were inhibited significantly by bavachin, while tissue inhibitors of metalloprotease were significantly upregulated. Bavachin inhibits the expression of inducible nitric oxide synthase, a representative catabolic factor, and downregulated the expression of nitric oxide, cyclooxygenase-2, and prostaglandin E2 in a dose-dependent manner in chondrocytes. Our results suggest that the bavachin has anabolic and potent anticatabolic biological effects on chondrocytes, which may have considerable promise in treating articular cartilage degeneration in the future.
authors.affiliation: Department of Prosthodontics, School of Dentistry, Chosun University.
authors.forename: Gyeong-Je
authors.initials: GJ
authors.lastname: Lee
authors.forename: In-A
authors.initials: IA
authors.lastname: Cho
authors.forename: Kyeong-Rok
authors.initials: KR
authors.lastname: Kang
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Hong-Moon
authors.initials: HM
authors.lastname: Sohn
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: You
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Oh
authors.forename: Yo-Seob
authors.initials: YS
authors.lastname: Seo
authors.forename: Sang-Joun
authors.initials: SJ
authors.lastname: Yu
authors.forename: Jae-Seek
authors.initials: JS
authors.lastname: You
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Im
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D015232:Dinoprostone / Q000378:metabolism; D019483:Disintegrins / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D053583:Interleukin-1beta / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy / Q000378:metabolism*; D048789:Phytoestrogens / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011509:Proteoglycans / Q000378:metabolism; D029907:Psoralea / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D019699:Thrombospondins / Q000378:metabolism
chemical_list: D024043:Collagen Type II; D052246:Cyclooxygenase 2 Inhibitors; D019483:Disintegrins; D005419:Flavonoids; D053583:Interleukin-1beta; D048789:Phytoestrogens; D010936:Plant Extracts; D011509:Proteoglycans; D019699:Thrombospondins; C459212:bavachin; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D020782:Matrix Metalloproteinases; D015232:Dinoprostone