title: Motility and fertilizing ability of cryopreserved Caspian brown trout (Salmo trutta caspius) sperm: Effect of post-thaw storage time and different sperm-to-egg ratios.
abstract: This study was designed to test the effect of post-thaw storage time on sperm motility parameters of Caspian brown trout (n=7). Furthermore, we investigated the effect of sperm-to-egg ratios of 100,000:1, 300,000:1 and 600,000:1 on fertility of cryopreserved Caspian brown semen. Quality was assessed by measuring sperm motility parameters and fertilization rates at the eyed and hatching stages. The percentage of post-thawed sperm motility, curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH) were not affected by 60 min of storage, whereas a decrease in straight line velocity (VSL), average path velocity (VAP) and linearity (LIN) were found in cryopreserved semen. Thus, the cryopreserved sperm of Caspian brown trout could be stored up to 60 min without loss of the percentage of sperm motility. The fertilization rate was not affected by 60 min of post-thaw storage and was over 70% for sperm-to-egg ratios of both 300,000 and 600,000:1. To our knowledge, this study is the first to report the high post-thaw fertilization ability of Caspian brown trout semen at a sperm-to-egg ratio as low as 300,000:1. This procedure after scaling up can be recommended for routine Caspian brown trout sperm cryopreservation.
authors.affiliation: Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Karim
authors.initials: K
authors.lastname: Golshahi
authors.affiliation: Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
authors.forename: Nariman
authors.initials: N
authors.lastname: Shabani
authors.affiliation: Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran. Electronic address: msaramli@gmail.com.
authors.forename: Mohammad Sadegh
authors.initials: MS
authors.lastname: Aramli
authors.affiliation: Biology Department, Faculty of Sciences, Mohaghegh Ardabili University, Ardabil, Iran.
authors.forename: Elnaz
authors.initials: E
authors.lastname: Noori
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005298:Fertility; D005306:Fertilization; D006801:Humans; D008297:Male; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology*; D013084:Sperm-Ovum Interactions; D013997:Time Factors; D014337:Trout / Q000502:physiology*
keywords: Caspian brown trout; Cryopreservation; Fertilization; Sperm