title: Natural killer cell and gamma delta T cell alterations in enthesitis related arthritis category of juvenile idiopathic arthritis.
abstract: Enthesitis related arthritis (ERA) is associated with increased frequency of Th17 cells and synovial fluid (SF) IL-17 levels. Natural killer (NK) and gamma delta T cells have been recently shown to produce IL-17, thus we studied the NK and gamma delta-T cells in peripheral blood (PB) of 50 ERA, 16 other JIA patients and 19 healthy controls. We have analyzed the frequency of NK (total, CD56dim, CD56bright) and gamma delta-T cells, perforin and KIR3DL1/2 expression on NK cells and IL-17 and IFN-gamma production by them using flow cytometry. ERA patients had more NK cells with reduced perforin expression and IFN-gamma production but increased KIR3DL1/2 expression and IL-17 production as compared to controls. Also IL-17 producing gamma delta-T were increased in PB of ERA patients. Paired SF samples had NK cells with reduced perforin and KIR3DL expression. Thus increased NK and gamma delta-T cells may contribute to the inflammation in ERA by producing IL-17.
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Gaur
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
authors.forename: Ramnath
authors.initials: R
authors.lastname: Misra
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India. Electronic address: amita@sgpgi.ac.in.
authors.forename: Amita
authors.initials: A
authors.lastname: Aggarwal
mesh_terms: D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D054353:Perforin / Q000276:immunology; D054341:Receptors, KIR3DL1 / Q000276:immunology; D054347:Receptors, KIR3DL2 / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: C508717:IL17A protein, human; D020381:Interleukin-17; C515083:KIR3DL1 protein, human; C515084:KIR3DL2 protein, human; D054341:Receptors, KIR3DL1; D054347:Receptors, KIR3DL2; D054353:Perforin; D007371:Interferon-gamma
keywords: Childhood arthritis; Inflammation; Innate immune cells; Killer immunoglobulin-like receptors; Perforin; Synovial fluid