title: Antibiofilm Activity of Chilean Propolis on Streptococcus mutans Is Influenced by the Year of Collection.
abstract: The chemical composition of propolis varies according to factors that could have an influence on its biological properties. Polyphenols from propolis have demonstrated an inhibitory effect on Streptococcus mutans growth. However, it is not known if different years of propolis collection may affect its activity. We aimed to elucidate if the year of collection of propolis influences its activity on Streptococcus mutans. Polyphenol-rich extracts were prepared from propolis collected in three different years, characterized by LC-MS and quantified the content of total polyphenols and flavonoids groups. Finally, was evaluated the antibacterial effect on Streptococcus mutans and the biofilm formation. Qualitative differences were observed in total polyphenols, flavones, and flavonols and the chemical composition between the extracts, affecting the strength of inhibition of biofilm formation but not the antimicrobial assays. In conclusion, chemical composition of propolis depends on the year of collection and influences the strength of the inhibition of biofilm formation.
authors.affiliation: Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile.
authors.forename: Jorge Jesús
authors.initials: JJ
authors.lastname: Veloz
authors.affiliation: Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Saavedra
authors.affiliation: Departamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile.
authors.forename: Alexis
authors.initials: A
authors.lastname: Lillo
authors.affiliation: Departamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile.
authors.forename: Marysol
authors.initials: M
authors.lastname: Alvear
authors.affiliation: Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile.
authors.forename: Leticia
authors.initials: L
authors.lastname: Barrientos
authors.affiliation: Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Avenida Francisco Salazar, 01145 Temuco, Chile.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Salazar
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002677:Chile; D004305:Dose-Response Relationship, Drug; D011429:Propolis / Q000008:administration & dosage* / Q000737:chemistry*; D012621:Seasons; D013295:Streptococcus mutans / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D011429:Propolis