title: Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer.
abstract: Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAK1 and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Gerçeker
authors.affiliation: Department of Medical Genetics and Medical Biology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Seda Orenay
authors.initials: SO
authors.lastname: Boyacıoglu
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Elmas
authors.initials: E
authors.lastname: Kasap
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Baykan
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Yuceyar
authors.affiliation: Department of Medical Genetics and Medical Biology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Yıldırım
authors.affiliation: Department of Pathology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Semin
authors.initials: S
authors.lastname: Ayhan
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Ender
authors.initials: E
authors.lastname: Ellidokuz
authors.affiliation: Department of Medical Genetics and Medical Biology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Korkmaz
mesh_terms: D000328:Adult; D000368:Aged; D064096:Aurora Kinase A / Q000096:biosynthesis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D003093:Colitis, Ulcerative / Q000150:complications / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D019175:DNA Methylation / Q000235:genetics; D044127:Epigenesis, Genetic / Q000235:genetics; D005260:Female; D042421:Histone Code / Q000235:genetics; D056284:Histone Deacetylase 1 / Q000096:biosynthesis / Q000235:genetics; D006655:Histone Deacetylases / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D054462:p21-Activated Kinases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D064096:Aurora Kinase A; C459456:NEK6 protein, human; D000072379:NIMA-Related Kinases; C515903:PAK1 protein, human; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases; C110550:HDAC1 protein, human; C543273:HDAC7 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases