title: Inhibitory effect of 2‑mercaptoethane sulfonate on the formation of Escherichia coli biofilms in vitro.
abstract: The biofilms (BF) formed by Escherichia coli (E. coli) is an important cause of chronic and recurrent infections due to its capacity to persist on medical surfaces and indwelling devices, demonstrating the importance of inhibiting the formation of E. coli BF and reducing BF infection. Although 2‑mercaptoethane sulfonate (MESNA) exhibits a marked mucolytic effect clinically, the effect of MESNA on the inhibition of E. coli BF formation remains to be elucidated. The present study investigated whether MESNA inhibits the formation of E. coli BF in vitro. The minimum inhibitory concentration of MESNA on E. coli was determined to be 10 mg/ml. Subsequently, the effect of MESNA on BF early adhesion, extracellular polysaccharide (EPS) and extracellular protein were detected. The effect of a subinhibitory concentration of MESNA on BF formation was evaluated, and the inhibitory potency of MESNA against matured BF was assayed. The results revealed that MESNA inhibited early stage adhesion and formation of the E. coli BF, destroyed the mature BF membrane and reduced the EPS and extracellular proteins levels of the BF. In addition, the present study investigated the effects of MESNA on the expression of EPS‑ and adhesion protein‑associated genes using quantitative polymerase chain reaction analysis, which demonstrated that MESNA effectively inhibited the expression of these genes. These results suggested that MESNA possesses anti‑BF formation capability on E. coli in vitro and may be used as a potential reagent for the clinical treatment of E. coli BF‑associated infections.
authors.affiliation: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Nianhai
authors.initials: N
authors.lastname: He
authors.affiliation: Department of Neonatology, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Neonatology, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Luquan
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Deng
authors.affiliation: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Shen
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D005110:Extracellular Space; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015080:Mesna / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011135:Polysaccharides, Bacterial / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D015080:Mesna