title: [High-throughput analysis of bacterial community of transition zone in littoral wetland of Wuliangsuhai eutrophic lake].
abstract: OBJECTIVE
  We studied soil bacterial community composition, abundance and diversity of transition zone along eutrophic lakeside wetland sediments and soils.
  
  
  METHODS
  The total DNA was extracted according to the sediment DNA extraction. Then high-throughput pyrosequencing was used to detect soil bacterial community composition,abundance and diversity based-on 16S rRNA gene. Soil physicochemical properties were tested to analyze its effects on bacterial community according to standard methods.
  
  
  RESULTS
  The soil bacterial community composition and relative abundance were very different across transition zone in littoral wetland. Bacteria groups mainly include Proteobacteria, Bacteroidetes, Chloroflexi, Actinobacteria, Planctomycetes and Gemmatimonadetes at phylum level. The diversity index of bacterial communities gradually increased according the land distribution, especially the phylum Proteobacteria and the genus Sulfurimonas. Correlation analysis indicated that the combination of total phosphorus, total water soluble salt and ammonium has the most significant effects on the whole bacterial community structure, and Mantel Test results indicated that the correlation was statistically significant (R = 0.8857, P = 0.037).
  
  
  CONCLUSIONS
  The bacterial community structure of transition zone is quite different in littoral wetland of Wuliangsuhai eutrophic lake, where Sulfurimonas play potential important roles in biogeochemical cycles of sediments in Wuliangsuhai Lake.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Li
authors.forename: Ruifang
authors.initials: R
authors.lastname: Du
authors.forename: Ji
authors.initials: J
authors.lastname: Zhao
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D005068:Eutrophication; D059014:High-Throughput Nucleotide Sequencing; D060106:Lakes / Q000737:chemistry / Q000382:microbiology*; D010802:Phylogeny; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D012987:Soil