title: Precursors and metabolic pathway for guaiacol production by Alicyclobacillus acidoterrestris.
abstract: Alicyclobacillus acidoterrestris has recently received much attention due to its implication in the spoilage of pasteurized fruit juices, which was manifested by the production of guaiacol. Vanillic acid and vanillin have been accepted as the biochemical precursors of guaiacol in fruit juices. The purpose of this study was to try to find other precursors and elucidate details about the conversion of vanillic acid and vanillin to guaiacol by A. acidoterrestris. Four potential substrates including ferulic acid, catechol, phenylalanine and tyrosine were analyzed, but they could not be metabolized to guaiacol by all the thirty A. acidoterrestris strains tested. Resting cell studies and enzyme assays demonstrated that vanillin was reduced to vanillyl alcohol by NADPH-dependent vanillin reductase and oxidized to vanillic acid by NAD(P)(+)-dependent vanillin dehydrogenases in A. acidoterrestris DSM 3923. Vanillic acid underwent a nonoxidative decarboxylation to guaiacol. The reversible vanillic acid decarboxylase involved was oxygen insensitive and pyridine nucleotide-independent.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: cairui@nwsuaf.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Cai
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Zhouli
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Laping
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yutang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yuetl305@nwsuaf.edu.cn.
authors.forename: Tianli
authors.initials: T
authors.lastname: Yue
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D056450:Alicyclobacillus / Q000201:enzymology / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D005516:Food Microbiology; D006139:Guaiacol / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D010088:Oxidoreductases / Q000378:metabolism; D014641:Vanillic Acid / Q000378:metabolism
chemical_list: D001547:Benzaldehydes; D006139:Guaiacol; C100058:vanillin; D010088:Oxidoreductases; D000445:Aldehyde Oxidoreductases; C105452:vanillin dehydrogenase; D002262:Carboxy-Lyases; D014641:Vanillic Acid
keywords: Alicyclobacillus acidoterrestris; Guaiacol; Metabolic pathway; Precursor; Vanillic acid; Vanillin