title: Anabaena sp. mediated bio-oxidation of arsenite to arsenate in synthetic arsenic (III) solution: Process optimization by response surface methodology.
abstract: Blue green algae Anabaena sp. was cultivated in synthetic arsenite solution to investigate its bio-oxidation potential for arsenic species. Response surface methodology (RSM) was employed based on a 3-level full factorial design considering four factors, viz. initial arsenic (III) concentration, algal dose, temperature and time. Bio-oxidation (%) of arsenic (III) was considered as response for the design. The study revealed that about 100% conversion of As (III) to As (V) was obtained for initial As (III) concentration of 2.5-7.5 mg/L at 30 °C for 72 h of exposure using 3 g/L of algal dose signifying a unique bio-oxidation potential of Anabaena sp. The dissolved CO2 (DCO2) and oxygen (DO) concentration in solution was monitored during the process and based on the data, a probable mechanism was proposed wherein algal cell acts like a catalytic membrane surface and expedites the bio-oxidation process. Bioaccumulation of arsenic, as well as, surface adsorption on algal cell was found considerably low. Lipid content of algal biomass grown in arsenite solution was found slightly lower than that of algae grown in synthetic media. Toxicity effects on algal cells due to arsenic exposure were evaluated in terms of comet assay and chlorophyll a content which indicated DNA damage to some extent along with very little decrease in chlorophyll a content. In summary, the present study explored the potential application of Anabaena sp. as an ecofriendly and sustainable option for detoxification of arsenic contaminated natural water with value-added product generation.
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Glass and Ceramic Research Institute, India; Ceramic Membrane Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja S.C. Mullick Road, Kolkata 700032, India.
authors.forename: Animesh
authors.initials: A
authors.lastname: Jana
authors.affiliation: Ceramic Membrane Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja S.C. Mullick Road, Kolkata 700032, India.
authors.forename: Priyankari
authors.initials: P
authors.lastname: Bhattacharya
authors.affiliation: Drug Development Diagnostics & Biotechnology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, India.
authors.forename: Snehasikta
authors.initials: S
authors.lastname: Swarnakar
authors.affiliation: Ceramic Membrane Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja S.C. Mullick Road, Kolkata 700032, India.
authors.forename: Swachchha
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Glass and Ceramic Research Institute, India; Ceramic Membrane Division, CSIR-Central Glass and Ceramic Research Institute, 196, Raja S.C. Mullick Road, Kolkata 700032, India. Electronic address: sourja@cgcri.res.in.
authors.forename: Sourja
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D000327:Adsorption; D017033:Anabaena / Q000378:metabolism*; D001149:Arsenates / Q000302:isolation & purification / Q000378:metabolism*; D001151:Arsenic / Q000737:chemistry*; D018053:Arsenites / Q000302:isolation & purification / Q000378:metabolism*; D002734:Chlorophyll / Q000031:analogs & derivatives / Q000378:metabolism; D000077194:Chlorophyll A; D004785:Environmental Pollutants / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D012996:Solutions
chemical_list: D001149:Arsenates; D018053:Arsenites; D004785:Environmental Pollutants; D012996:Solutions; D002734:Chlorophyll; C085815:chlorophyll a'; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid; D000077194:Chlorophyll A
keywords: Anabaena sp.; Arsenic; Bio-oxidation; Blue green algae; Response surface methodology (RSM)