title: High prevalence of bla OXA-23 in Acinetobacter spp. and detection of bla NDM-1 in A. soli in Cuba: report from National Surveillance Program (2010-2012).
abstract: As a first national surveillance of Acinetobacter in Cuba, a total of 500 Acinetobacter spp. isolates recovered from 30 hospitals between 2010 and 2012 were studied. Acinetobacter baumannii-calcoaceticus complex accounted for 96.4% of all the Acinetobacter isolates, while other species were detected at low frequency (A. junii 1.6%, A. lwoffii 1%, A. haemolyticus 0.8%, A. soli 0.2%). Resistance rates of isolates were 34-61% to third-generation cephalosporins, 49-50% to β-lactams/inhibitor combinations, 42-47% to aminoglycosides, 42-44% to carbapenems and 55% to ciprofloxacin. However, resistance rates to colistin, doxycycline, tetracycline and rifampin were less than 5%. Among carbapenem-resistant isolates, 75% harboured different bla OXA genes (OXA-23, 73%; OXA-24, 18%; OXA-58, 3%). The bla NDM-1 gene was identified in an A. soli strain, of which the species was confirmed by sequence analysis of 16S rRNA gene, rpoB, rpoB-rpoC and rpoL-rpoB intergenic spacer regions and gyrB. The sequences of bla NDM-1 and its surrounding genes were identical to those reported for plasmids of A. baumannii and A. lwoffi strains. This is the first report of bla NDM-1 in A. soli, together with a high prevalence of OXA-23 carbapenemase for carbapenem resistance in Acinetobacter spp. in Cuba.
authors.affiliation: Tropical Medicine Institute 'Pedro Kourí', Havana City, Cuba.
authors.forename: D
authors.initials: D
authors.lastname: Quiñones
authors.affiliation: Tropical Medicine Institute 'Pedro Kourí', Havana City, Cuba.
authors.forename: I
authors.initials: I
authors.lastname: Carvajal
authors.affiliation: Tropical Medicine Institute 'Pedro Kourí', Havana City, Cuba.
authors.forename: Y
authors.initials: Y
authors.lastname: Perez
authors.affiliation: 'Hermanos Ameijeiras' Hospital, Havana City, Cuba.
authors.forename: M
authors.initials: M
authors.lastname: Hart
authors.affiliation: Pediátrico 'J. M. Márquez' Hospital, Havana City, Cuba.
authors.forename: J
authors.initials: J
authors.lastname: Perez
authors.affiliation: 'V. I. Lenin' Hospital, Holguín, Holguín, Cuba.
authors.forename: S
authors.initials: S
authors.lastname: Garcia
authors.affiliation: Tropical Medicine Institute 'Pedro Kourí', Havana City, Cuba.
authors.forename: D
authors.initials: D
authors.lastname: Salazar
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan ; Department of Biomedical Science, Ross University School of Medicine, St Kitts, West Indies.
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawaguchiya
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: M S
authors.initials: MS
authors.lastname: Aung
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
keywords: Acinetobacter; Cuba; NDM-1; OXA-23; carbapenem resistance