title: In vitro digestion and fermentation properties of linear sugar-beet arabinan and its oligosaccharides.
abstract: This study was conducted to investigate the prebiotic effects of linear arabino-oligosaccharides (LAOS) and debranched (linear) sugar beet arabinan (LAR) for the development of new prebiotics. LAOS were prepared from LAR by enzymatic hydrolysis with endo-arabinanase from Bacillus licheniformis, followed by removal of the arabinose fraction by incubation with resting cells of Leuconostoc mesenteroides. The resulting LAOS contained DP2 (28.7%), DP3 (49.9%), DP4 (20.1%), and DP5 (1.16%). A standardized digestibility test showed that LAOS and LAR were not digestible. Individual cultures of 24 strains of gastrointestinal bacteria showed that LAOS and LAR stimulated growth of Lactobacillus brevis, Bifidobacterium longum, and Bacteroides fragilis. In vitro batch fermentation using human fecal samples showed that LAOS had higher bifidogenic properties than LAR; LAOS increased the population of bifidobacteria which produced short-chain fatty acids (SCFAs). LAOS was fermented slowly compared to fructo-oligosaccharides and this may permit SCFA production in the distal colon. This study demonstrates that LAOS prepared from LAR are promising dietary substrates for improvement of human intestinal health.
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Moon
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Shin
authors.affiliation: Department of Agro-food Resource, National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea.
authors.forename: Hye Sun
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Wooha
authors.initials: W
authors.lastname: Joo
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Seung Kee
authors.initials: SK
authors.lastname: Cho
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Tae-Jip
authors.initials: TJ
authors.lastname: Kim
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea. Electronic address: namsoo@chungbuk.ac.kr.
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D027461:Beta vulgaris / Q000737:chemistry*; D005232:Fatty Acids, Volatile / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D019344:Lactic Acid / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D056692:Prebiotics; D025341:Principal Component Analysis
chemical_list: D005232:Fatty Acids, Volatile; D009844:Oligosaccharides; D011134:Polysaccharides; D056692:Prebiotics; C030080:araban; D019344:Lactic Acid
keywords: Digestibility; In vitro fermentation; Linear arabino-oligosaccharides (LAOS); Prebiotics; Sugar-beet arabinan