title: Effects of fluoride on microtubule ultrastructure and expression of Tubα1a and Tubβ2a in mouse hippocampus.
abstract: Axonal and dendrictic degenerations were observed in non-skeleton fluorosis as the neurological manifestations. Microtubules, composed of the assembled tubulin dimers, are the essential cytoskeleton of axon and dendron. However, the effect of fluoride (F) on microtubules status and tubulin dimer expression in central nerves system remains largely unknown. In this study, the ultrastructure of microtubules and expression of Tubα1a and Tubβ2a were detected in hippocampus of mice orally administrated with 25, 50, or 100mgL(-1) NaF for 60d. Results showed that in F treatment groups, microtubules were broken into discrete fragments and bended, which were no longer stretched and went along the axon well. In addition, the expression of Tubα1a and Tubβ2a on both gene and protein levels were significantly reduced in high F group. The visual results of immunocytochemistry also confirmed the decreased protein expressions of Tubα1a and Tubβ2a. These findings suggested that microtubule lesions could be an important cause for neurodegeneration observed in fluorosis, and F may threaten the microtubule stability by affecting the expression of tubulin dimers.
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Niu
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Xue
authors.affiliation: College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Biology, Taiyuan Normal University, Taiyuan 030031, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yan
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Xuying
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China. Electronic address: ndfcp@163.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Feng
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China. Electronic address: wangjd53@outlook.com.
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure*; D012969:Sodium Fluoride / Q000494:pharmacology*; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism*
chemical_list: C569741:Tuba1a protein, mouse; D014404:Tubulin; D012969:Sodium Fluoride
keywords: Fluoride; Hippocampus; Microtubule; Tubα1a; Tubβ2a