title: Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio).
abstract: The aim of this study was to investigate the effects of naproxen on the gene expression of antioxidant enzymes in adult zebrafish. Surprisingly, after 2 weeks exposure no significant effect on the mRNA expression of the target genes was found in the liver. However, mRNA levels of three genes were altered significantly in the intestine. The expression of Ucp-2 decreased at the environmental concentration of 1μg/L while mRNA expression of GST p2 increased at the concentration of 100μg/L. The mRNA level for the antioxidant enzyme CAT was up-regulated significantly at both the concentrations used. Exposure to naproxen caused only moderate effects on the expression of antioxidant genes in the intestine rather than in the liver, which demonstrates that the intestine is more sensitive to waterborne naproxen exposure than the liver. Interestingly, the adverse side effects of NSAIDs occur in the gastrointestinal tract of humans. To our knowledge, this is the first study that has focused on transcriptional effects of naproxen on zebrafish.
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackehotr. 1/3, 61242 Brno, Czech Republic. Electronic address: stancova@itpark.cz.
authors.forename: V
authors.initials: V
authors.lastname: Stancová
authors.affiliation: Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ziková
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackehotr. 1/3, 61242 Brno, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Svobodová
authors.affiliation: Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany; Department of Endocrinology, Institute of Biology, Humboldt-University, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Kloas
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics*; D007422:Intestines / Q000201:enzymology*; D007473:Ion Channels / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D024101:Mitochondrial Proteins / Q000235:genetics*; D009288:Naproxen / Q000009:adverse effects*; D000071246:Uncoupling Protein 2; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2; D029961:Zebrafish Proteins; D009288:Naproxen; D002374:Catalase; D005982:Glutathione Transferase
keywords: Fish; Naproxen; Oxidative stress; mRNA expression