title: Phytochrome-interacting factors PIF4 and PIF5 negatively regulate anthocyanin biosynthesis under red light in Arabidopsis seedlings.
abstract: Light is an important environmental factor inducing anthocyanin accumulation in plants. Phytochrome-interacting factors (PIFs) have been shown to be a family of bHLH transcription factors involved in light signaling in Arabidopsis. Red light effectively increased anthocyanin accumulation in wild-type Col-0, whereas the effects were enhanced in pif4 and pif5 mutants but impaired in overexpression lines PIF4OX and PIF5OX, indicating that PIF4 and PIF5 are both negative regulators for red light-induced anthocyanin accumulation. Consistently, transcript levels of several genes involved in anthocyanin biosynthesis and regulatory pathway, including CHS, F3'H, DFR, LDOX, PAP1 and TT8, were significantly enhanced in mutants pif4 and pif5 but decreased in PIF4OX and PIF5OX compared to in Col-0, indicating that PIF4 and PIF5 are transcriptional repressor of these gene. Transient expression assays revealed that PIF4 and PIF5 could repress red light-induced promoter activities of F3'H and DFR in Arabidopsis protoplasts. Furthermore, chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) test and electrophoretic mobility shift assay (EMSA) showed that PIF5 could directly bind to G-box motifs present in the promoter of DFR. Taken together, these results suggest that PIF4 and PIF5 negatively regulate red light-induced anthocyanin accumulation through transcriptional repression of the anthocyanin biosynthetic genes in Arabidopsis.
authors.affiliation: College of Earth and Environmental Sciences, Lanzhou University, Lanzhou, Gansu 73000, People's Republic of China; Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Zhongjuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Chengzhou
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Yadi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China. Electronic address: yrbi@lzu.edu.cn.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Bi
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant; D008027:Light; D008954:Models, Biological; D059372:Nucleotide Motifs / Q000235:genetics; D000073718:Pancreatitis-Associated Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism / Q000528:radiation effects*; D014158:Transcription, Genetic / Q000528:radiation effects
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger; C459466:PIF4 protein, Arabidopsis; C497729:PIF5 protein, Arabidopsis; C000612141:REG3A protein, human
keywords: Anthocyanin; Arabidopsis; PIFs; Red light