title: Central action of ELABELA reduces food intake and activates arginine vasopressin and corticotropin-releasing hormone neurons in the hypothalamic paraventricular nucleus.
abstract: ELABELA (ELA) is a novel hormone consisting of 32 amino acid peptides found in humans as well as other vertebrates and is considered to play an important role in the circulatory system through the apelin receptor (APJ). However, whether ELA also acts in the central nervous system remains unknown. Here, we show that ELA functions as an anorexigenic hormone in adult mouse brain. An intracerebroventricular injection of ELA reduces food intake and activates arginine vasopressin (AVP) and corticotropin-releasing hormone (CRH) neurons in the paraventricular nucleus (PVN), a hypothalamic region that regulates food intake. Cytosolic calcium ([Ca]i) measurement shows that ELA dose dependently increases [Ca]i in single AVP and CRH-immunoreactive neurons isolated from the PVN. Our data suggest that ELA functions as an anorexigenic hormone through activation of AVP and CRH neurons in the PVN.
authors.affiliation: aBiology Department, Faculty of Mathematics and Natural Sciences, Andalas University, Padang, Indonesia bDepartment of Electrophysiology and Oncology cDepartment of Organ Regulatory Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Putra
authors.initials: P
authors.lastname: Santoso
authors.forename: Yuko
authors.initials: Y
authors.lastname: Maejima
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kumamoto
authors.forename: Seiichi
authors.initials: S
authors.lastname: Takenoshita
authors.forename: Kenju
authors.initials: K
authors.lastname: Shimomura
mesh_terms: D000818:Animals; D001067:Appetite Depressants / Q000494:pharmacology*; D001127:Arginine Vasopressin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002408:Catheters, Indwelling; D002413:Cations, Divalent / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry; D057967:Infusions, Intraventricular; D008297:Male; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects* / Q000378:metabolism; D036361:Peptide Hormones / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism
chemical_list: C000601133:APELA protein, human; D001067:Appetite Depressants; D002413:Cations, Divalent; D036361:Peptide Hormones; D016760:Proto-Oncogene Proteins c-fos; D001127:Arginine Vasopressin; D003346:Corticotropin-Releasing Hormone; D002118:Calcium