title: Determination of the relative percentage distribution of THCA and Δ(9)-THC in herbal cannabis seized in Austria - Impact of different storage temperatures on stability.
abstract: Cannabis is globally by far the most widespread illicit drug of abuse. Especially since its legalization in some of the US, controversies about the legal status of cannabis for recreational and medical use have come up. Δ(9)-Tetrahydrocannabinol (Δ(9)-THC), which is the major active ingredient in cannabis products, is mainly responsible for the psychoactive effects. Its inactive biosynthetic precursor tetrahydrocannabinolic acid (THCA) is present in different quantities in fresh and undried cannabis plants. Under influence of drying, temperature and UV exposure it decomposes to Δ(9)-THC. In this study, a quantification of Δ(9)-THC and THCA was carried out to check the stability of cannabis samples. The determination of the degradation of THCA to Δ(9)-THC in 29 cannabis products seized in Austria was monitored by HPLC-UV. Mobile phase consisted of a 25mM triethylammoniumphosphate buffer (pH 3.0) and acetonitrile (36:64). A common LiChrospher(®) 100 RP-18 column was utilized as stationary phase. To check the influence of low as well as high temperature on the degradation process of the cannabinoid THCA to Δ(9)-THC, samples were stored in a freezer or in a drying cabinet for a specified time period. It was shown successfully that high storage temperatures led to a more rapid and complete decomposition of THCA to Δ(9)-THC while at low temperatures only slight or no changes of the percentage distribution were determined.
authors.affiliation: Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1, A-8010 Graz, Austria.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Taschwer
authors.affiliation: Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1, A-8010 Graz, Austria. Electronic address: martin.schmid@uni-graz.at.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Schmid
mesh_terms: D001317:Austria; D002188:Cannabis / Q000737:chemistry*; D002853:Chromatography, Liquid; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000032:analysis*; D004355:Drug Stability; D004356:Drug Storage; D013696:Temperature
chemical_list: D013759:Dronabinol; C025351:delta(9)-tetrahydrocannabinolic acid
keywords: Content determination; High-performance liquid chromatography; Stability test under temperature influence; THCA; Δ(9)-Tetrahydrocannabinol (Δ(9)-THC)