title: Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.
abstract: Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Yong Joon
authors.initials: YJ
authors.lastname: Jeong
authors.affiliation: Department of Food and Nutrition, Hanyang University, Seoul, Republic of Korea.
authors.forename: Inhye
authors.initials: I
authors.lastname: Kim
authors.affiliation: Department of Biological & Environmental Science, Dongguk University, Seoul, Republic of Korea.
authors.forename: Joon Hyung
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Park
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Kwon
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Moon Won
authors.initials: MW
authors.lastname: Jung
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Xue
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Se Min
authors.initials: SM
authors.lastname: Jo
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Hae Seong
authors.initials: HS
authors.lastname: Song
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Cho
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
authors.forename: Sang Mok
authors.initials: SM
authors.lastname: Song
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Ham
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Jung
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
authors.forename: Chang Sook
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
authors.forename: Weon-Jong
authors.initials: WJ
authors.lastname: Yoon
authors.affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D019807:Iodoacetic Acid; D032862:Litsea / Q000737:chemistry*; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics; D015394:Molecular Structure; D010003:Osteoarthritis / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics; D055114:X-Ray Microtomography
chemical_list: D016207:Cytokines; D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D000431:Ethanol; D020782:Matrix Metalloproteinases; D019807:Iodoacetic Acid