title: Protective activity of salidroside against ethanol-induced gastric ulcer via the MAPK/NF-κB pathway in vivo and in vitro.
abstract: Salidroside (Sal) is a traditional Chinese medicine with various pharmacological effects. The present study aimed to investigate the protective effect of Sal on ethanol-induced acute gastric ulcer and H2O2-induced gastric epithelial cell damage. 0.2 ml ethanol and 400 μM H2O2 were applied to establish a gastric ulcer model in vivo and in vitro respectively. The production of interleukin (IL)-6, interleukin (IL)-1β and tumor necrosis factor (TNF)-α was analyzed, as well as myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD). MTT assay was used to detect cell viability. In addition, MAPK/NF-κB signal pathway-related proteins p-ERK, p-JNK, p-p38, p-IκBα and p-NF-κBp65 were analyzed to determine the underlying protective mechanism. Downstream genes such as cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) and leukotrienes B4 (LTB4) were also measured. Obtained data indicated that Sal inhibited the overproduction of pro-inflammatory cytokines and enhanced antioxidant activity. Collectively, it is assumed that Sal could alleviate ethanol-induced acute gastric ulcer and H2O2-induced gastric epithelial cell damage through the MAPK/NF-κB pathway.
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Xiayun
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Luo
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Lingpeng
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: He
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wei
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Gong
authors.affiliation: Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China. Electronic address: yantianhuabest@126.com.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Yan
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D000431:Ethanol; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007975:Leukotriene B4 / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000897:Anti-Ulcer Agents; D016207:Cytokines; D005960:Glucosides; D016328:NF-kappa B; D010636:Phenols; D007975:Leukotriene B4; D000431:Ethanol; D006861:Hydrogen Peroxide; D001094:Arachidonate 5-Lipoxygenase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D020928:Mitogen-Activated Protein Kinases; C009172:rhodioloside
keywords: Ethanol; Gastric ulcer; Inflammation; MAPKs/NF-κB; Salidroside