title: Novel mechanism of gene transfection by low-energy shock wave.
abstract: Extracorporeal shock wave (SW) therapy has been studied in the transfection of naked nucleic acids into various cell lines through the process of sonoporation, a process that affects the permeation of cell membranes, which can be an effect of cavitation. In this study, siRNAs were efficiently transfected into primary cultured cells and mouse tumor tissue via SW treatment. Furthermore SW-induced siRNA transfection was not mediated by SW-induced sonoporation, but by microparticles (MPs) secreted from the cells. Interestingly, the transfection effect of the siRNAs was transferable through the secreted MPs from human umbilical vein endothelial cell (HUVEC) culture medium after treatment with SW, into HUVECs in another culture plate without SW treatment. In this study, we suggest for the first time a mechanism of gene transfection induced by low-energy SW through secreted MPs, and show that it is an efficient physical gene transfection method in vitro and represents a safe therapeutic strategy for site-specific gene delivery in vivo.
authors.affiliation: Department of Asan Institute for Life Sciences, Asan Medical Center, College of Medicine, University of Ulsan, 86 Asanbyeoungwon-gil, Songpa-gu, Seoul, 138-736, Korea.
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Ha
authors.affiliation: Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea.
authors.forename: Seok Cheol
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea.
authors.forename: Sunghyen
authors.initials: S
authors.lastname: Kim
authors.affiliation: Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea.
authors.forename: Jihwa
authors.initials: J
authors.lastname: Chung
authors.affiliation: Department of Rehabilitation Medicine, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea.
authors.forename: Hasuk
authors.initials: H
authors.lastname: Bae
authors.affiliation: 1] Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea [2] Department of Internal Medicine, Cardiology Division, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea.
authors.forename: Kihwan
authors.initials: K
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001011:Aorta; D045744:Cell Line, Tumor; D055252:Cell-Derived Microparticles / Q000378:metabolism; D055785:Gene Knockdown Techniques; D019220:High-Energy Shock Waves; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000517:prevention & control; D018919:Neovascularization, Physiologic; D061251:Primary Cell Culture; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A