title: Levels of S100B in brain and blood of rats with diabetic ketoacidosis.
abstract: Diabetic ketoacidosis (DKA) frequently causes subtle brain injuries in children. Rarely, these injuries can be severe and life threatening. The physiological processes leading to brain injury during DKA are poorly understood. S100B is a calcium-binding protein secreted by astrocytes. Elevated serum S100B levels are documented in several types of brain injuries. S100B may have either neuroprotective or neurotoxic effects, depending upon the concentration. We undertook the current studies to measure alterations in S100B production and secretion during DKA. We measured serum S100B concentrations in juvenile rats during and after DKA, and used immunohistochemistry to measure S100B expression in the hippocampus, cortex and striatum. Compared to levels in both normal and hyperglycemic control rats, serum S100B levels during DKA were significantly reduced. Serum S100B gradually rose after DKA, returning to levels of hyperglycemic controls by 72 h. S100B expression in the hippocampus was also significantly reduced 24h after DKA. There were no significant changes in S100B expression in other brain regions. Our findings contrast with those for other types of brain injuries in which both serum S100B levels and astrocyte S100B expression are typically elevated. These data suggest that serum S100B measurement cannot be used as an indicator of brain injury during DKA. Whether reduced S100B production or secretion is involved in the pathogenesis of DKA-related brain injury should be investigated.
authors.affiliation: Department of Pediatrics, University of California Davis, School of Medicine, Sacramento, CA 96817, USA. Electronic address: nsglaser@ucdavis.edu.
authors.forename: Nicole
authors.initials: N
authors.lastname: Glaser
authors.affiliation: Department of Pediatrics, University of California Davis, School of Medicine, Sacramento, CA 96817, USA.
authors.forename: Weei
authors.initials: W
authors.lastname: Lo
authors.affiliation: Department of Pediatrics, University of California Davis, School of Medicine, Sacramento, CA 96817, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tancredi
authors.affiliation: Department of Pediatrics, University of California Davis, School of Medicine, Sacramento, CA 96817, USA.
authors.forename: Myra
authors.initials: M
authors.lastname: Orgain
authors.affiliation: Department of Biomedical Engineering, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA.
authors.forename: Vikram
authors.initials: V
authors.lastname: Puvenna
authors.affiliation: Department of Biomedical Engineering, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA; Cerebrovascular Research, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA; Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA.
authors.forename: Damir
authors.initials: D
authors.lastname: Janigro
authors.affiliation: Physiology and Membrane Biology, University of California Davis, School of Medicine, Sacramento, CA 96817, USA.
authors.forename: Martha
authors.initials: M
authors.lastname: O Donnell
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D016883:Diabetic Ketoacidosis / Q000097:blood* / Q000139:chemically induced / Q000473:pathology*; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006943:Hyperglycemia / Q000097:blood / Q000209:etiology / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit / Q000378:metabolism*; D013311:Streptozocin / Q000633:toxicity; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D005904:Glial Fibrillary Acidic Protein; D064568:S100 Calcium Binding Protein beta Subunit; D013311:Streptozocin
keywords: Brain injury; Cerebral edema; Diabetic ketoacidosis; Pediatric; S100B