title: AtTCTP2 mRNA and protein movement correlates with formation of adventitious roots in tobacco.
abstract: The Translationally Controlled Tumor Proteins, or TCTP, is a superfamily of exclusively eukaryotic proteins essential in the regulation of proliferation and general growth. However, it is clear that these are multifunctional proteins given (1) the pleiotropic effects of its mutations, and (2), the multiple processes in which this protein is involved. TCTP function in general is conserved, since Arabidopsis AtTCTP1 can rescue a Drosophila mutant, and vice versa. It has become clear, however, that these proteins may have "taxon-specific" functions. In the case of plants, mRNA and/or proteins have been found in the phloem translocation stream of different species, suggesting a role in long-distance signaling. We have found that a second Arabidopsis TCTP gene, AtTCTP2, codes for a protein that moves long-distance through a graft union in tobacco. Interestingly, the mRNA is also transported long-distance. Both mRNA and protein move long-distance; interestingly, the movement, while more efficient from source to sink tissues, also occurs in the opposite direction. The protein reaches the nuclei of parenchyma cells and adventitious roots. Furthermore, it is clear that the long-distance delivery of AtTCTP2 protein and mRNA is required for the induction of adventitious roots. A model is presented that accounts for these observations.
authors.affiliation: a Department of Biotechnology and Bioengineering ; CINVESTAV ; Zacatenco, Mexico DF , Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Toscano-Morales
authors.affiliation: a Department of Biotechnology and Bioengineering ; CINVESTAV ; Zacatenco, Mexico DF , Mexico.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Xoconostle-Cázares
authors.affiliation: a Department of Biotechnology and Bioengineering ; CINVESTAV ; Zacatenco, Mexico DF , Mexico.
authors.forename: Angélica Concepción
authors.initials: AC
authors.lastname: Martínez-Navarro
authors.affiliation: a Department of Biotechnology and Bioengineering ; CINVESTAV ; Zacatenco, Mexico DF , Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ruiz-Medrano
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000032:analysis; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008954:Models, Biological; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C000602137:TCTP protein, Arabidopsis; D049452:Green Fluorescent Proteins
keywords: Agrobacterium rhizogenes; RNA and protein long-distance transport; TCTP; adventitious roots; plant regeneration