title: Transcriptional Profiling ofMycobacterium tuberculosisReplicating in the Human Type II Alveolar Epithelial Cell Line, A549.
abstract: Alveolar epithelial cells outnumber alveolar macrophages by ~500 fold and increasing evidence suggestsMycobacterium tuberculosismay replicate dramatically in these cells during the initial weeks of infecting the lung [1,2]. Here, we report in experimental detail the transcriptional profiling ofMycobacterium tuberculosisreplicating at 72 hr post-infection in the human type II alveolar epithelial cell line, A549, as compared toMycobacterium tuberculosisgrowing logarithmically in laboratory broth culture [2]. All resulting transcriptional profiling data was deposited to the Gene Expression Omnibus (GEO) database under the accession number GSE58466.
authors.affiliation: Department of Pathology, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Michelle B
authors.initials: MB
authors.lastname: Ryndak
authors.affiliation: Department of Pathology, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Singh
authors.affiliation: Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Veterans Affairs New York Harbor Healthcare System, New York, New York, United States of America ; Department of Pathology, New York University Langone Medical Center, New York, New York, United States of America.
authors.forename: Suman
authors.initials: S
authors.lastname: Laal
keywords: A549; Mycobacterium tuberculosis; host/pathogen interactions; microarray