title: Detection of Protein-Protein Interactions in Tobacco BY-2 Cells Using Bimolecular Fluorescence Complementation.
abstract: Knowledge of protein-protein interactions in the plant cell is invaluable for furthering our understanding of the functions of these proteins. Many of the methods available for the study of these interactions, such as yeast two-hybrid and co-immunoprecipitation assays, rely on in vitro techniques. Here we describe the use of bimolecular fluorescence complementation for the study of protein-protein interactions in vivo, using simple techniques and accessible materials.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 108 N. Greene St., Baltimore, MD, 21201, USA, gcook@som.umaryland.edu.
authors.forename: Gemma S
authors.initials: GS
authors.lastname: Puts
authors.forename: Natasha
authors.initials: N
authors.lastname: Spadafora
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D014026:Nicotiana / Q000166:cytology*; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria