title: A system biology approach for understanding the miRNA regulatory network in colon rectal cancer.
abstract: In this paper we present a systems biology approach to the understanding of the miRNA-regulatory network in colon rectal cancer. An initial set of significant genes in Colon Rectal Cancer (CRC) were obtained by mining relevant literature. An initial set of cancer-related miRNAs were obtained from three databases: miRBase, miRWalk, Targetscan and GEO microarray experiment. First principle methods were then used to generate the global miRNA-gene network. Significant miRNAs and associated transcription factors in the global miRNA-gene network were identified using topological and sub-graph analyses. Eleven novel miRNAs were identified and three of the novel miRNAs, hsa-miR-630, hsa-miR-100 and hsa-miR-99a, were further analysed to elucidate their role in CRC. The proposed methodology effectively made use of literature data and was able to show novel, significant miRNA-transcription associations in CRC.
authors.forename: Meeta
authors.initials: M
authors.lastname: Pradhan
authors.forename: Kshithija
authors.initials: K
authors.lastname: Nagulapalli
authors.forename: Lakenvia
authors.initials: L
authors.lastname: Ledford
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Pandit
authors.forename: Mathew
authors.initials: M
authors.lastname: Palakal
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics*; D057225:Data Mining / Q000379:methods; D030541:Databases, Genetic; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D049490:Systems Biology / Q000379:methods; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; D014157:Transcription Factors