title: Oxidation of glyceraldehyde-3-phosphate dehydrogenase decreases sperm motility in diabetes mellitus.
abstract: The relation between the activity of the sperm specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) and the motility of sperms in diabetic men was investigated. It was found that the mean value of GAPDS activity and sperm motility in diabetic sperm samples is lower than that in normal samples. The activity of GAPDS protein was significantly positive correlation with sperm motility (R = 0.96) and significantly negative correlation with the incidence of infertility (R = -0.99). The fluorescence intensity of GAPDS protein in sperms of 2-diabetic patients (>5years, n = 88) was significantly lower than that in normal control group. The location rate of GAPDS protein in sperms of diabetic men was also significantly lower compared with that found in samples from non-diabetic men. Exogenous GAPDS protein can diminish ROS content in sperms and promote sperm motility at the concentration of over 5 ug/ml GAPDS. Based on the literature data on the importance of GAPDS for the motility of sperms in diabetic men, it was concluded that the decrease in the sperm motility and GAPDS activity in the presence of high glucose or diabetes mellitus is due to oxidation of GAPDS and inhibition of glycolysis by the reactive oxygen species produced by sperms.
authors.affiliation: Biochip Laboratory, Yantai Yu-Huang-Ding Hospital, Qingdao University, Yantai 264000, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Biochip Laboratory, Yantai Yu-Huang-Ding Hospital, Qingdao University, Yantai 264000, Shandong, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biochip Laboratory, Yantai Yu-Huang-Ding Hospital, Qingdao University, Yantai 264000, Shandong, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gong
authors.affiliation: Biochip Laboratory, Yantai Yu-Huang-Ding Hospital, Qingdao University, Yantai 264000, Shandong, China. Electronic address: liulocus@126.com.
authors.forename: Chengming
authors.initials: C
authors.lastname: Sun
mesh_terms: D016022:Case-Control Studies; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D015870:Gene Expression; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D007248:Infertility, Male / Q000150:complications / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008297:Male; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013081:Sperm Motility / Q000235:genetics*; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species; C579041:GAPDHS protein, human; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
keywords: Diabetes mellitus; GAPDS; Glucose; Sperm