title: Therapeutic effect of Chinese patent medicine "Wuhuanghu" on porcine infectious pleuropneumonia and its acute and subchronic toxicity as well as evaluation of safety pharmacology.
abstract: Chinese patent medicines play an important role in veterinary clinical use. The aim of this study is to research the anti-infection effect of Chinese patent medicine "Wuhuanghu" for the treatment of porcine infectious pleuropneumonia and to evaluate the safety of "Wuhuanghu" in order to provide a comprehensive understanding of its toxicity. The anti-infection results showed that the treatment with "Wuhuanghu" could significantly inhibit pneumonia and decrement of the pneumonia in high, medium and low doses of "Wuhuanghu" groups were 70.97%, 61.29% and 58.06% respectively. The acute toxicity test showed that rats in the highest group (5000mg/kg) had no death and no abnormal response, suggesting the LD50 of "Wuhuanghu" was more than 5000mg/kg. The subchronic toxicity study showed that hematology indexes in all groups had no obvious differences; blood biochemical index, only albumin and total cholesterol in middle and low doses of "Wuhuanghu" groups were significantly decreased when compared with control group. The clinical pathology showed that the target organ of "Wuhuanghu" was liver. The safety pharmacology study indicated that "Wuhuanghu" had no side effects on rats. In conclusion, "Wuhuanghu" has therapeutic and protective effects to porcine infectious pleuropneumonia in a dose-dependent manner and "Wuhuanghu" is a safe veterinary medicine.
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Guangxi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Kang
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: yinzhongq@163.com.
authors.forename: Zhongqiong
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Ren-Yong
authors.initials: RY
authors.lastname: Jia
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lai
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Liang
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Li
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yuan-Feng
authors.initials: YF
authors.lastname: Zou
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lv
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Chang-Liang
authors.initials: CL
authors.lastname: He
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ye
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Li-Zi
authors.initials: LZ
authors.lastname: Yin
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jing
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D008099:Liver / Q000187:drug effects; D008297:Male; D004366:Nonprescription Drugs / Q000008:administration & dosage* / Q000009:adverse effects; D011001:Pleuropneumonia / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D051381:Rats; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000473:pathology; D023382:Toxicity Tests, Acute; D059550:Toxicity Tests, Subchronic; D019155:Veterinary Drugs / Q000008:administration & dosage / Q000009:adverse effects
chemical_list: D004365:Drugs, Chinese Herbal; D004366:Nonprescription Drugs; D019155:Veterinary Drugs
keywords: Acute toxicity; Anti-infection; Chinese patent medicine “Wuhuanghu”; Porcine infectious pleuropneumonia; Safety pharmacology; Subchronic toxicity