title: An insight into antimicrobial activity of the freshwater bryozoan Pectinatella magnifica.
abstract: The antimicrobial activity of five crude extracts of the freshwater bryozoan Pectinatella magnifica (Leidy, 1851) was evaluated in vitro for the first time. P. magnifica acetone extract exhibited the highest antibacterial activity (minimum inhibitory concentrations (MIC) 0.004-0.350 mg/mL and MBC 0.007-0.500 mg/mL), while its methanol extract showed the most promising antifungal activity (MIC 0.03-0.12 mg/mL and MFC 0.06-0.25 mg/mL). Furthermore, at a concentration of 0.25 MIC, the methanol extract reduced biofilm formation of the bacterial strain Pseudomonas aeruginosa PAO1 in a considerable extent (59.14%). FTIR spectra of the most active extracts indicate the presence of carbonyl compounds, long-chain alcohols and/or sterols. According to the experimental data obtained, P. magnifica methanol extract may be considered as a good resource of novel natural products with potent antibiofilm activity against the bacterium well known for its resistance.
authors.affiliation: a Department of Life Sciences , Institute for Multidisciplinary Research - IMSI, University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology , Institute for Biological Research "Sinisa Stankovic", University of Belgrade , Belgrade , Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Ciric
authors.affiliation: c Faculty of Sciences, Department of Biology and Ecology , University of Novi Sad , Novi Sad , Serbia.
authors.forename: Mladen
authors.initials: M
authors.lastname: Horvatovic
authors.affiliation: c Faculty of Sciences, Department of Biology and Ecology , University of Novi Sad , Novi Sad , Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Jurca
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology , Institute for Biological Research "Sinisa Stankovic", University of Belgrade , Belgrade , Serbia.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamoclija
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology , Institute for Biological Research "Sinisa Stankovic", University of Belgrade , Belgrade , Serbia.
authors.forename: Milos
authors.initials: M
authors.lastname: Nikolic
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology , Institute for Biological Research "Sinisa Stankovic", University of Belgrade , Belgrade , Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
mesh_terms: D000096:Acetone; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002013:Bryozoa / Q000737:chemistry*; D000432:Methanol; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D000096:Acetone; D000432:Methanol
keywords: Bryozoa; Pseudomonas aeruginosa PAO1; antibiofilm activity; extracts