title: Poly(dopamine) coating of 3D printed poly(lactic acid) scaffolds for bone tissue engineering.
abstract: 3D printing is a versatile technique to generate large quantities of a wide variety of shapes and sizes of polymer. The aim of this study is to develop functionalized 3D printed poly(lactic acid) (PLA) scaffolds and use a mussel-inspired surface coating to regulate cell adhesion, proliferation and differentiation of human adipose-derived stem cells (hADSCs). We prepared PLA 3D scaffolds coated with polydopamine (PDA). The chemical composition and surface properties of PDA/PLA were characterized by XPS. PDA/PLA modulated hADSCs' responses in several ways. Firstly, adhesion and proliferation, and cell cycle of hADSCs cultured on PDA/PLA were significantly enhanced relative to those on PLA. In addition, the collagen I secreted from cells was increased and promoted cell attachment and cell cycle progression were depended on the PDA content. In osteogenesis assay, the ALP activity and osteocalcin of hADSCs cultured on PDA/PLA were significantly higher than seen in those cultured on pure PLA scaffolds. Moreover, hADSCs cultured on PDA/PLA showed up-regulation of the ang-1 and vWF proteins associated with angiogenic differentiation. Our results demonstrate that the bio-inspired coating synthetic PLA polymer can be used as a simple technique to render the surfaces of synthetic scaffolds active, thus enabling them to direct the specific responses of hADSCs.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Lin
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Yeh
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan; Department of Thoracic Surgery, China Medical University Hospital, Taichung City, Taiwan; School of Medicine, College of Medicine, College of Public Health, Taichung City, Taiwan.
authors.forename: Hsin-Yuan
authors.initials: HY
authors.lastname: Fang
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D066330:Printing, Three-Dimensional; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D024042:Collagen Type I; D007211:Indoles; D011091:Polyesters; D011108:Polymers; C568283:polydopamine; D015675:Osteocalcin; D019344:Lactic Acid; C033616:poly(lactide)
keywords: 3D printed-scaffold; Angiogenic; Dopamine; Osteogenic; Poly(lactic acid); Tissue engineering