title: Induced All-Female Autotriploidy in the Allotetraploids of Carassius auratus red var. (♀) × Megalobrama amblycephala (♂).
abstract: Following activation by UV-irradiated BSB sperm, the fertilized eggs of tetraploid hybrids (abbreviated as 4nF1) (4n = 148, AABB) of Carassius auratus red var. (abbreviated as RCC) (2n = 100, AA) (♀) × Megalobrama amblycephala (abbreviated as BSB) (2n = 48, BB) (♂) developed into normal live gynogenetic offspring without chromosome doubling treatment. Some of these were autotriploids with three sets of red crucian carp chromosomes (abbreviated as G1) (3n = 150, AAA). G1 were all-females, and can produce unreduced (3n) eggs at age 1 year. After activation by UV-irradiated BSB sperm, the fertilized eggs of G1 developed into a second generation of autotriploid gynogenetic offspring (abbreviated as G2) (3n = 150) without chromosome doubling treatment. G1 were obviously different from both 4nF1 and RCC in their morphological traits and showed a significantly higher growth rate than RCC. In aquaculture, the autotriploid fish could provide an important source of gametes for the production of all-female triploid fish and for the establishment of autotriploid gynogenetic lines.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, People's Republic of China.
authors.forename: Qinbo
authors.initials: Q
authors.lastname: Qin
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Dai
authors.forename: YuDe
authors.initials: Y
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics*; D005260:Female; D006054:Goldfish / Q000235:genetics*; D006824:Hybridization, Genetic; D008297:Male; D011003:Ploidies; D057885:Triploidy