title: Cortical Differentiation of Human Pluripotent Cells for In Vitro Modeling of Alzheimer's Disease.
abstract: Stem cell models of Alzheimer's disease provide an opportunity to study the mechanisms underlying disease pathology at a resolution that is not possible in animal models. Furthermore, the ability to reprogram patient somatic cells to a pluripotent state ensures that the disease can be investigated in the correct genetic context. Here, we describe the directed differentiation of human pluripotent cells to cortical progenitors by recapitulating key developmental signaling events in vitro. Over a timeframe that mirrors human development, these progenitors give rise to functional lower and upper layer neurons. We also describe biochemical and imaging based methods to analyse key APP and Tau phenotypes in neurons generated from pluripotent stem cells from individuals with either monogenic familial Alzheimer's disease or Down's syndrome.
authors.affiliation: Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
authors.forename: Nathalie G
authors.initials: NG
authors.lastname: Saurat
authors.forename: Frederick J
authors.initials: FJ
authors.lastname: Livesey
authors.forename: Steven
authors.initials: S
authors.lastname: Moore
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002540:Cerebral Cortex / Q000473:pathology*; D015925:Cryopreservation; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D005971:Glutamates / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D046569:Neuroepithelial Cells / Q000166:cytology
chemical_list: D016229:Amyloid beta-Peptides; D005971:Glutamates