title: Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy.
abstract: Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of O−P−O asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.
authors.affiliation: Manipal University, Department of Atomic and Molecular Physics, Manipal 576 104, India.
authors.forename: Surekha
authors.initials: S
authors.lastname: Barkur
authors.affiliation: Manipal University, Department of Atomic and Molecular Physics, Manipal 576 104, India.
authors.forename: Aseefhali
authors.initials: A
authors.lastname: Bankapur
authors.affiliation: UM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Mumbai 400 098, India.
authors.forename: Madhura
authors.initials: M
authors.lastname: Pradhan
authors.affiliation: Manipal University, Department of Atomic and Molecular Physics, Manipal 576 104, India.
authors.forename: Santhosh
authors.initials: S
authors.lastname: Chidangil
authors.affiliation: Tata Institute of Fundamental Research, 1 Homi Bhabha Road, Mumbai 400 005, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Mathur
authors.affiliation: UM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Mumbai 400 098, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Ladiwala
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000737:chemistry* / Q000473:pathology*; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*