title: Stability of ubiquinol-10 (reduced form of coenzyme Q10 ) in human blood.
abstract: The ratio of ubiquinol-10 in total coenzyme Q10 (TQ10 ) in human plasma has been proposed as a useful biomarker of oxidative stress. Since ubiquinol-10 is easily oxidized in air, it is necessary to perform suitable processing at medical institutions prior to analysis. To establish stable storage conditions for blood to determine the ubiquinol-10/TQ10 ratios properly, the effects of temperature conditions on the stability of ubiquinol-10 were studied. Blood samples were collected from nine male Japanese volunteers. Changes in ubiquinol-10/TQ10 ratios in blood samples were evaluated under three temperature conditions (room temperature, refrigerated and ice-cooled). Plasma levels of ubiquinol-10 and ubiquinone-10 were determined by an HPLC system with electrochemical detection and the ubiquinol-10/TQ10 ratios were calculated. We found that the ubiquinol-10/TQ10 ratio was stable up to 8 or 4 h when blood samples were stored in refrigerator or ice-cold container, respectively, and its decreases during these periods were <1.0%. We conclude that, in order to evaluate ubiquinol-10/TQ10 ratios, blood samples should be stored in a refrigerator or an ice-cold container, and processed for plasma separation within 4 h.
authors.affiliation: Takasago Analysis Center, Kaneka Techno Research Corporation 1-8 Takasago-cho Miyamae-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuo
authors.affiliation: Takasago Analysis Center, Kaneka Techno Research Corporation 1-8 Takasago-cho Miyamae-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kasai
authors.affiliation: QOL Division, KANEKA Corporation 2-3-18 Nakanosima, Kita-ku Osaka, 530-8288, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Hosoe
authors.affiliation: QOL Division, KANEKA Corporation 2-3-18 Nakanosima, Kita-ku Osaka, 530-8288, Japan.
authors.forename: Iwao
authors.initials: I
authors.lastname: Funahashi
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001793:Blood Preservation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003080:Cold Temperature; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013048:Specimen Handling; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D015415:Biomarkers; D014451:Ubiquinone; C026663:ubiquinol-10; C024989:coenzyme Q10
keywords: plasma; stability; ubiquinol-10; ubiquinone-10