title: A Self-Assembled DNA Origami-Gold Nanorod Complex for Cancer Theranostics.
abstract: A self-assembled DNA origami (DO)-gold nanorod (GNR) complex, which is a dual-functional nanotheranostics constructed by decorating GNRs onto the surface of DNA origami, is demonstrated. After 24 h incubation of two structured DO-GNR complexes with human MCF7 breast cancer cells, significant enhancement of cell uptake is achieved compared to bare GNRs by two-photon luminescence imaging. Particularly, the triangle shaped DO-GNR complex exhibits optimal cellular accumulation. Compared to GNRs, improved photothermolysis against tumor cells is accomplished for the triangle DO-GNR complex by two-photon laser or NIR laser irradiation. Moreover, the DO-GNR complex exhibits enhanced antitumor efficacy compared with bare GNRs in nude mice bearing breast tumor xenografts. The results demonstrate that the DO-GNR complex can achieve optimal two-photon cell imaging and photothermal effect, suggesting a promising candidate for cancer diagnosis and therapy both in vitro and in vivo.
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchial Fabrication, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Institute of Automation, Chinese Academy of Science, 95 ZhongGuanCun East Road, Beijing, 100190, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchial Fabrication, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchial Fabrication, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhan
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchial Fabrication, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Song
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchial Fabrication, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, China.
authors.forename: Luru
authors.initials: L
authors.lastname: Dai
authors.affiliation: Institute of Automation, Chinese Academy of Science, 95 ZhongGuanCun East Road, Beijing, 100190, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Automation, Chinese Academy of Science, 95 ZhongGuanCun East Road, Beijing, 100190, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Radiology, Stanford University, 1201 Welch Road, Lucas Center, P095, Stanford, CA, 94305, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchial Fabrication, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Ding
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced; D061986:MCF-7 Cells; D051379:Mice; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000628:therapy*; D009690:Nucleic Acid Conformation; D010789:Phototherapy; D013056:Spectrophotometry, Ultraviolet; D000068936:Theranostic Nanomedicine
chemical_list: D006046:Gold; D004247:DNA
keywords: DNA origami; gold nanorods; photothermal therapy; self-assembly; theranostics