title: [Blockade of AT1 receptor of Angiotensin II reduces the number of antral follicles in female rats with obesity induced by cafeteria diet].
abstract: OBJECTIVE
  To evaluate the follicular development of female Wistar rats with obesity induced by the cafeteria diet, submitted to the administration of losartan (LOS), an antagonist of the AT1 receptor of Angiotensin II.
  
  
  METHODS
  At weaning (21 days of age), female Wistar rats were randomly divided, into two groups: control (CTL) that received standard chow and cafeteria (CAF) that received a cafeteria diet, a highly palatable and highly caloric diet. At 70 days of age, at the beginning of the reproductive age, animals of the CAF group were subdivided into two groups (n = 15/group): CAF, that received water, and CAF+LOS, that received LOS for 30 days. The CTL group also received water by gavage. At 100 days of age, the animals were euthanized and body weight (BW) as well as the retroperitoneal, perigonadal and subcutaneous fat weights were analyzed. The right ovaries were isolated for counting the number of primary, secondary, antral and mature follicles. Plasma levels of FSH, LH, prolactin and progesterone hormones were analyzed. The results were expressed as mean ± standard error of the mean. Data were analyzed statistically by one-way ANOVA followed by the Newman-Keuls post-test (p < 0.05).
  
  
  RESULTS
  BW and fat weight, as well as the number of antral follicles, were higher in the CAF group compared to the CTL group. However, FSH and LH levels were lower in CAF animals compared to CTL animals. LOS administration attenuated the reduction of FSH and LH levels. Progesterone and PRL levels were similar among groups.
  
  
  CONCLUSIONS
  LOS could improve follicular development in obese females and could be used as an adjunctive drug in the treatment of infertility associated with obesity.
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Sara Cristina
authors.initials: SC
authors.lastname: Sagae
authors.affiliation: Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Cristiane Gisselda
authors.initials: CG
authors.lastname: Gobo
authors.affiliation: Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Edson Duarte Ribeiro
authors.initials: ED
authors.lastname: Paz
authors.affiliation: Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Juliana Beal
authors.initials: JB
authors.lastname: Menegotto
authors.affiliation: Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Patrícia Kanae
authors.initials: PK
authors.lastname: Yamashita
authors.affiliation: Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Celso Rodrigues
authors.initials: CR
authors.lastname: Franci
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
authors.forename: Sandra Lucinei
authors.initials: SL
authors.lastname: Balbo
mesh_terms: D000804:Angiotensin II; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D000818:Animals; D004032:Diet; D002149:Energy Intake; D005260:Female; D005522:Food Services; D019808:Losartan / Q000494:pharmacology*; D009765:Obesity / Q000503:physiopathology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D000804:Angiotensin II; D019808:Losartan