title: The Q705K and F359L Single-Nucleotide Polymorphisms of NOD-Like Receptor Signaling Pathway: Association with Chronic Pancreatitis, Pancreatic Cancer, and Periodontitis.
abstract: The aim of this study was to establish the correlation between the occurrence of Q705K and F359L polymorphisms in patients diagnosed with pancreatic diseases and periodontal conditions of various degrees of severity. The above-mentioned genetic markers were assessed in patients with pancreatic cancer (n = 18) and chronic pancreatitis (n = 39) as well as in a healthy control group (n = 115). The established inclusion criteria were the following: Caucasian descent, non-smoking, and age range 20-80, with different levels of periodontitis activity according to S. Offenbacher's scale. The genotyping reactions were performed by means of an RT-PCR with the use of TaqMan(®) genotyping assay. Results of the study revealed that the state of periodontium was significantly worse in patients with chronic pancreatitis. The Q705K and F359L polymorphisms were associated with more advanced cases of periodontitis measured by clinical attachment level, whereas the Q705K was associated with intensified bleeding index. Furthermore, the F359L single-nucleotide polymorphism was significantly higher in the group with chronic pancreatitis (p < 0.0001; OR = 6.8571). Whereas, the prevalence of Q705K polymorphism was higher in the group of pancreatic cancer (p = 0.107; OR = 3.3939). This study suggests that the exaggerated inflammatory response provoked by Q705K and F359L might be the common denominator for periodontitis, pancreatic cancer, and chronic pancreatitis. These findings might constitute the basis for a new diagnostic and therapeutic approach.
authors.affiliation: Department of Periodontology, Medical University of Warsaw, Miodowa 18, 00-246, Warsaw, Poland. andrzej.miskiewicz@wum.edu.pl.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Miskiewicz
authors.affiliation: Department of Pathology, Medical Univeristy of Warsaw, Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Szparecki
authors.affiliation: Department of Gastroenterological and Transplant Surgery, Central Clinical Hospital, Ministry of Internal Affairs, Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Durlik
authors.affiliation: Department of Gastroenterology, Central Clinical Hospital, Ministry of Internal Affairs, Warsaw, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Rydzewska
authors.affiliation: Department of Gastroenterological and Transplant Surgery, Central Clinical Hospital, Ministry of Internal Affairs, Warsaw, Poland.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Ziobrowski
authors.affiliation: Department of Periodontology, Medical University of Warsaw, Miodowa 18, 00-246, Warsaw, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Górska
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology*; D010195:Pancreatitis / Q000235:genetics / Q000276:immunology*; D010518:Periodontitis / Q000235:genetics / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide; D015398:Signal Transduction / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D016207:Cytokines; D018836:Inflammation Mediators; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C495732:NLRP2 protein, human; C453881:NLRP3 protein, human
keywords: Chronic pancreatitis; Inflammasomes; Inflammation mediators; Innate immunity; Pancreatic cancer; Periodontitis