title: Cloning and Sequence Analysis of Recombinant Plasmodium vivax Merozoite Surface Protein 1 (PvMSP-142 kDa) In pTZ57R/T Vector.
abstract: BACKGROUND
  Carboxy-terminal 42 kDa region of Plasmodium vivax merozoite surface protein-1 is considered as an important antigen in blood stage. Since, this region has been observed to be polymorphic among isolates of P. vivax, it is significant to survey on different regions of this antigen in various areas of the world.
  
  
  METHODS
  In the present study, the genetic diversity of cloned PvMSP-142 kDa gene from an Iranian patient is analyzed. Parasite DNA was extracted from a P. vivax - infected patient in Iran. The region of PvMSP-142 kDa was amplified by PCR, cloned into pTZ57R/T vector and then sequenced.
  
  
  RESULTS
  Sequencing of cloned PvMSP-142 kDa gene clearly has a high degree of homology (95%) with reference Sal-I sequence and also with the homogeneous sequences from some studied countries (97%). Thirty eight SNPs (single nucleotide polymorphism) were identified in cloned PvMSP-142 kDa gene which the mutations had localized in the 33 kDa fragment (PvMSP-133 kDa), while there was nearly no variation in the 19 kDa fragment (PvMSP-119 kDa). 2 out of 38 mutations were found as to be novel haplotypes.
  
  
  CONCLUSIONS
  High similarity of cloned PvMSP-142 kDa gene in comparison to reference sequence and other sequences could be beneficial as a remarkable molecular marker for serological diagnostic kits of P. vivax in malarious neighboring countries of Iran and around the world.
authors.affiliation: Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Mirahmadi
authors.affiliation: Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Adel
authors.initials: A
authors.lastname: Spotin
authors.affiliation: Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Shirzad
authors.initials: S
authors.lastname: Fallahi
authors.affiliation: Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Niloofar
authors.initials: N
authors.lastname: Taghipour
authors.affiliation: Infectious and Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
authors.forename: Habibollah
authors.initials: H
authors.lastname: Turki
authors.affiliation: Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyyed Javad
authors.initials: SJ
authors.lastname: Seyyed Tabaei
keywords: Iran; Plasmodium vivax; Recombinant MSP-1 42 kDa; Sequencing