title: TAPP1 inhibits the differentiation of oligodendrocyte precursor cells via suppressing the Mek/Erk pathway.
abstract: Oligodendrocytes (OLs) are glial cells that form myelin sheaths around axons in the central nervous system (CNS). Loss of the myelin sheath in demyelinating and neurodegenerative diseases can lead to severe impairment of movement. Understanding the extracellular signals and intracellular factors that regulate OL differentiation and myelination during development can help to develop novel strategies for enhancing myelin repair in neurological disorders. Here, we report that TAPP1 was selectively expressed in differentiating OL precursor cells (OPCs). TAPP1 knockdown promoted OL differentiation and myelin gene expression in culture. Conversely, over-expression of TAPP1 in immature OPCs suppressed their differentiation. Moreover, TAPP1 inhibition in OPCs altered the expression of Erk1/2 but not AKT. Taken together, our results identify TAPP1 as an important negative regulator of OPC differentiation through the Mek/Erk signaling pathway.
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Ruyi
authors.initials: R
authors.lastname: Mei
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Teng
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Aifen
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, 40202, USA.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Zunyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. m0qiu001@yahoo.com.|Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, 40202, USA. m0qiu001@yahoo.com.
authors.forename: Mengsheng
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. xiaofengzhao@yahoo.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009836:Oligodendroglia / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C461495:Plekha1 protein, mouse
keywords: TAPP1; differentiation; oligodendrocyte; spinal cord