title: Adipocyte aminopeptidases in obesity and fasting.
abstract: This study checked the existence of a diverse array of aminopeptidase (AP) enzymes in high (HDM) and low (LDM) density microsomal and plasma membrane (MF) fractions from adipocytes of control, monosodium glutamate obese and food deprived rats. Gene expression was detected for ArgAP, AspAP, MetAP, and two AlaAP (APM and PSA). APM and PSA had the highest catalytic efficiency, whereas AspAP the highest affinity. Subcellular distribution of AP activities depended on metabolic status. Comparing catalytic levels, AspAP in HDM, LDM and MF was absent in obese and control under food deprivation; PSA in LDM was 3.5-times higher in obese than in normally fed control and control and obese under food deprivation; MetAP in MF was 4.5-times higher in obese than in food deprived obese. Data show new AP enzymes genetically expressed in subcellular compartments of adipocytes, three of them with altered catalytic levels that respond to whole-body energetic demands.
authors.affiliation: Laboratory of Pharmacology, Instituto Butantan, Av. Vital Brasil, 1500, 05503-900, Sao Paulo, Brazil; Department of Physiology, Universidade de Sao Paulo, Rua do Matao, Travessa 14, 101, 05508-900, Sao Paulo, Brazil.
authors.forename: Rafaela Fadoni
authors.initials: RF
authors.lastname: Alponti
authors.affiliation: Laboratory of Pharmacology, Instituto Butantan, Av. Vital Brasil, 1500, 05503-900, Sao Paulo, Brazil. Electronic address: paulo.silveira@butantan.gov.br.
authors.forename: Paulo Flavio
authors.initials: PF
authors.lastname: Silveira
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000201:enzymology; D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D005215:Fasting / Q000097:blood / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D008861:Microsomes / Q000201:enzymology; D009765:Obesity / Q000097:blood / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics; D051381:Rats; D012970:Sodium Glutamate / Q000009:adverse effects*
chemical_list: D000626:Aminopeptidases; D012970:Sodium Glutamate
keywords: Adipocyte; Aminopeptidases; ERAP; Food deprivation; Obesity