title: A programmable microfluidic static droplet array for droplet generation, transportation, fusion, storage, and retrieval.
abstract: We present a programmable microfluidic static droplet array (SDA) device that can perform user-defined multistep combinatorial protocols. It combines the passive storage of aqueous droplets without any external control with integrated microvalves for discrete sample dispensing and dispersion-free unit operation. The addressable picoliter-volume reaction is systematically achieved by consecutively merging programmable sequences of reagent droplets. The SDA device is remarkably reusable and able to perform identical enzyme kinetic experiments at least 30 times via automated cross-contamination-free removal of droplets from individual hydrodynamic traps. Taking all these features together, this programmable and reusable universal SDA device will be a general microfluidic platform that can be reprogrammed for multiple applications.
authors.affiliation: Department of Chemical Engineering, Chungnam National University, Daejeon, Republic of Korea. rhadum@cnu.ac.kr.
authors.forename: Si Hyung
authors.initials: SH
authors.lastname: Jin
authors.forename: Heon-Ho
authors.initials: HH
authors.lastname: Jeong
authors.forename: Byungjin
authors.initials: B
authors.lastname: Lee
authors.forename: Sung Sik
authors.initials: SS
authors.lastname: Lee
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
mesh_terms: D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods