title: Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes.
abstract: A family of cationic Pluronic-based polyrotaxanes (PR(+)), threaded with 2-hydroxypropyl-β-cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR(+) formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR(+):pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles. Charge density and threading efficiency of the PR(+) agent significantly influenced the colloidal stability and physical properties of the complexes, which impacted their intracellular transfection efficiencies. Taken together, our results suggest that HPCD: Pluronic PR(+) can be used as potent vectors for pDNA-based therapeutics.
authors.affiliation: Department of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
authors.forename: Vivek
authors.initials: V
authors.lastname: Badwaik
authors.affiliation: Department of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
authors.forename: Yawo
authors.initials: Y
authors.lastname: Mondjinou
authors.affiliation: Department of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
authors.forename: Aditya
authors.initials: A
authors.lastname: Kulkarni
authors.affiliation: Department of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
authors.forename: Linjia
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
authors.forename: Asher
authors.initials: A
authors.lastname: Demoret
authors.affiliation: Department of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA. davethom@purdue.edu.
authors.forename: David H
authors.initials: DH
authors.lastname: Thompson
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002460:Cell Line; D006801:Humans; D010957:Plasmids; D020442:Poloxamer / Q000737:chemistry*; D043862:Rotaxanes / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D043862:Rotaxanes; D047392:beta-Cyclodextrins; D020442:Poloxamer; D000073738:2-Hydroxypropyl-beta-cyclodextrin
keywords: gene delivery; pDNA; polyrotaxane; transfection; β-cyclodextrins