title: Liquid chromatography mass spectrometry-based O-glycomics to evaluate glycosylation alterations in gastric cancer.
abstract: OBJECTIVE
  Gastric cancer is the fourth most common malignant cancer worldwide. Important for tumorigenesis and progression, aberrant glycosylation occurs frequently in cancers. We investigated the differences in O-glycosylation in the serum of 157 gastric cancer patients (GC) and 144 healthy donors.
  
  
  METHODS
  We used the method of labeling O-glycans (released from proteins) with 1-phenyl-3-methyl-5-pyrazolone followed by LC-MS analysis. Analyzing the LC-MS data by partial least squares discriminant and unpaired Student t test, combined with the structural information of O-glycans from LC-MS/MS in positive mode.
  
  
  RESULTS
  The expression level of core1, core2, ST antigen, and core2 complex O-glycans (m/z 733.33, m/z 809.42) were increased significantly (p < 0.0001), whereas m/z 529.75 and diST-antigen were decreased in the serum of GC compared with controls (p < 0.001). In addition, there were significant correlations between the abundance of the O-glycans and glycoproteins (MUC1, CEA) in the serum of GC.
  
  
  CONCLUSIONS
  Glycomics approaches identified multiple candidate antigens for patients with GC. The O-glycan structures are increased in the serum of GC, they may be candidates for carbohydrate tumor markers.
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, P. R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, P. R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, P. R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, Huaian, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Suzhou Zhongying Medical Sciences and Technologies Company, Suzhou, P. R. China.
authors.forename: Xiukun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Suzhou Pharmavan Cancer Research Center Company, Suzhou, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, P. R. China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Miao
authors.affiliation: Department of Chinese Pharmaceutics, School of Chinese Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China.
authors.forename: Zijun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institutes of Biology and Medical Sciences, Soochow University, Suzhou, P. R. China.
authors.forename: Yunsen
authors.initials: Y
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016002:Discriminant Analysis; D054794:Glycomics; D006031:Glycosylation; D006801:Humans; D016018:Least-Squares Analysis; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000097:blood / Q000378:metabolism*
chemical_list: D011134:Polysaccharides
keywords: 1-Phenyl-3-methyl-5-pyrazolone; Gastric cancer; LC-MS; O-glycan; Serum