title: MicroRNA-486 as a Biomarker for Early Diagnosis and Recurrence of Non-Small Cell Lung Cancer.
abstract: BACKGROUND
  Non-small cell lung cancer (NSCLC) is a leading cause of cancer death worldwide. Early diagnosis is essential for improvements of prognosis and survival of the patients. Currently, there is no effective biomarker available in clinical settings for early detection of lung cancer. Altered expressions in many cancer types including NSCLC and stable existence in plasma make microRNAs (miRNAs) a group of potentially useful biomarkers for clinical assessments of patients with NSCLC.
  
  
  OBJECTIVE
  To evaluate the potential values of miRNAs as blood-based biomarkers for early diagnosis and prognosis in NSCLC patients.
  
  
  METHODS
  Peripheral blood samples from healthy volunteers and early-staged NSCLC patients before and after surgery were collected, and plasma was separated. Expression of ten miRNAs in the plasma and tumor sections of the patients was detected by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  MiRNA (miR)-486 and miR-150 were found to significantly distinguish lung cancer patients from healthy volunteers. Area under curve of miR-486 and miR-150 were 0.926 (sensitivity, 0.909; specificity, 0.818) and 0.752 (sensitivity, 0.818; specificity, 0.818), respectively. In response to therapy, patients with down-regulated miR-486 expression showed prolonged recurrence-free survival than those with un-reduced miR-486 expression (median, unreached vs. 19 months; hazard ratio, 0.1053; 95% confidence interval, 0.01045 to 1.060; P=0.056).
  
  
  CONCLUSIONS
  The results suggest that miR-486 and miR-150 could be potential blood-based biomarkers for early diagnosis of NSCLC. Monitoring change of miR-486 expression in plasma might be an effective and non-invasive method for recurrence prediction of early-staged NSCLC patients.
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Wanshuai
authors.initials: W
authors.lastname: Li
authors.affiliation: The Department of Cardiothoracic Surgery, No. 2 People's Hospital of Changzhou, 29 Xinglong Xiang, Changzhou, Jiangsu, 213004, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tang
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liu
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Huang
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China; GoPath Laboratories LLC, 1351 Barclay Blvd, Buffalo Grove, Illinois, 60089, United States of America.
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Changzhou GoPath Diagnostic Laboratory Co. Ltd, 801 Changwuzhong Road, Changzhou, Jiangsu, 213164, China; GoPath Laboratories LLC, 1351 Barclay Blvd, Buffalo Grove, Illinois, 60089, United States of America.
authors.forename: Jim
authors.initials: J
authors.lastname: Lu
authors.affiliation: The Department of Cardiothoracic Surgery, No. 2 People's Hospital of Changzhou, 29 Xinglong Xiang, Changzhou, Jiangsu, 213004, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yuan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D055088:Early Detection of Cancer; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C533097:MIR150, human; C560945:MIRN486 microRNA, human