title: Synergistic amylomaltase and branching enzyme catalysis to suppress cassava starch digestibility.
abstract: Starch provides our main dietary caloric intake and over-consumption of starch-containing foods results in escalating life-style disease including diabetes. By increasing the content of α-1,6 branch points in starch, digestibility by human amylolytic enzymes is expected to be retarded. Aiming at generating a soluble and slowly digestible starch by increasing the content and changing the relative positioning of the branch points in the starch molecules, we treated cassava starch with amylomaltase (AM) and branching enzyme (BE). We performed a detailed molecular analysis of the products including amylopectin chain length distribution, content of α-1,6 glucosidic linkages, absolute molecular weight distribution and digestibility. Step-by-step enzyme catalysis was the most efficient treatment, and it generated branch structures even more extreme than those of glycogen. All AM- and BE-treated samples showed increased resistance to degradation by porcine pancreatic α-amylase and glucoamylase as compared to cassava starch. The amylolytic products showed chain lengths and branching patterns similar to the products obtained from glycogen. Our data demonstrate that combinatorial enzyme catalysis provides a strategy to generate potential novel soluble α-glucan ingredients with low dietary digestibility assets.
authors.affiliation: School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand; Department of Plant and Environmental Sciences, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Waraporn
authors.initials: W
authors.lastname: Sorndech
authors.affiliation: Carlsberg Laboratory, Copenhagen V, Copenhagen, Denmark.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Meier
authors.affiliation: Carlsberg Laboratory, Copenhagen V, Copenhagen, Denmark.
authors.forename: Anita M
authors.initials: AM
authors.lastname: Jansson
authors.affiliation: Department of Plant and Environmental Sciences, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Domenico
authors.initials: D
authors.lastname: Sagnelli
authors.affiliation: Carlsberg Laboratory, Copenhagen V, Copenhagen, Denmark.
authors.forename: Ole
authors.initials: O
authors.lastname: Hindsgaul
authors.affiliation: School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand. Electronic address: s-tongta@sut.ac.th.
authors.forename: Sunanta
authors.initials: S
authors.lastname: Tongta
authors.affiliation: Department of Plant and Environmental Sciences, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: abl@plen.ku.dk.
authors.forename: Andreas
authors.initials: A
authors.lastname: Blennow
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000378:metabolism*; D000818:Animals; D055162:Biocatalysis; D002850:Chromatography, Gel; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005936:Glucans / Q000032:analysis; D006004:Glycogen Debranching Enzyme System / Q000378:metabolism*; D002365:Manihot / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013213:Starch / Q000737:chemistry* / Q000378:metabolism; D013552:Swine; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D005936:Glucans; D006004:Glycogen Debranching Enzyme System; D013213:Starch; D015061:1,4-alpha-Glucan Branching Enzyme; C020569:4 alpha-glucanotransferase; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Amylomaltase; Branched glucan; Branching enzyme; Clean modification; Slow carbohydrate