title: Changing Biosynthetic Profiles by Expressing bldA in Streptomyces Strains.
abstract: Recently we described an unusual way of activating a cryptic gene cluster when we explored the origin of the bald phenotype of Streptomyces calvus. Complementation of S. calvus with a correct copy of bldA restored sporulation and additionally promoted production of a new natural products. In this study we report on the expression of bldA in several Streptomyces strains that have been described as "poorly sporulating" strains. In seven out of 15 cases, HPLC profiling revealed the production of new compounds, and in two cases the overproduction of known compounds. Two compounds were isolated and their structures were determined.
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany.
authors.forename: Arne
authors.initials: A
authors.lastname: Gessner
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Heitzler
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany.
authors.forename: Songya
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Klaus
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany.
authors.forename: Renato
authors.initials: R
authors.lastname: Murillo
authors.affiliation: Department of Chemistry, Queen's University, Kingston, ON, K7L 3N6, Canada.
authors.forename: Hanna
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Queen's University, Kingston, ON, K7L 3N6, Canada.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Vanner
authors.affiliation: Department of Chemistry, Queen's University, Kingston, ON, K7L 3N6, Canada. dlzechel@chem.queensu.ca.
authors.forename: David L
authors.initials: DL
authors.lastname: Zechel
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany. andreas.bechthold@pharamzie.uni-freiburg.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D053898:Biosynthetic Pathways / Q000235:genetics; D020869:Gene Expression Profiling; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism*; D012356:RNA, Transfer, Leu / Q000235:genetics* / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C107344:BldA tRNA, Streptomyces coelicolor; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu
keywords: antibiotics; bldA; gene clusters; gene expression; natural products