title: Phyto mediated biogenic synthesis of silver nanoparticles using leaf extract of Andrographis echioides and its bio-efficacy on anticancer and antibacterial activities.
abstract: The present study reveals the efficiency of Andrographis echioides for green synthesis of silver nanoparticles (AgNPs). The leaf aqueous extract of A. echioides was used for the synthesis of AgNPs and they were characterized by UV-visible, High Resonance Scanning Electron Microscope (HRSEM), Energy-Dispersive X-ray Spectroscopy (EDX), Atomic Force Microscope (AFM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The toxicity of AgNPs was evaluated by using MTT assay. Our present study showed that biosynthesized AgNPs inhibited proliferation of human breast adenocarcinoma cancer cell line (MCF-7) with 31.5 μg/mL at 24h incubation. Results suggest that AgNPs may exert its anticancer activity on MCF-7 cell line by suppressing its growth. The silver nanoparticles was studied against Gram positive and Gram negative bacteria. The highest antibacterial activity was found against Escherichia coli (28 mm) and Staphylococcus aureus (23 mm) respectively.
authors.affiliation: CAS in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Elangovan
authors.affiliation: Department of Zoology, Presidency College (Autonomous), Chennai 600005, Tamilnadu, India.
authors.forename: D
authors.initials: D
authors.lastname: Elumalai
authors.affiliation: CAS in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Anupriya
authors.affiliation: CAS in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Shenbhagaraman
authors.affiliation: Department of Zoology, Presidency College (Autonomous), Chennai 600005, Tamilnadu, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Kaleena
authors.affiliation: CAS in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India. Electronic address: kmkmn92@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Murugesan
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D055772:Green Chemistry Technology; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts; D012834:Silver
keywords: Andrographis echioides; Antibacterial; MCF-7 cell line; Microscopic studies; Silver nanoparticles