title: Alteration of Membrane Compositional Asymmetry by LiCoO2 Nanosheets.
abstract: Given the projected massive presence of redox-active nanomaterials in the next generation of consumer electronics and electric vehicle batteries, they are likely to eventually come in contact with cell membranes, with biological consequences that are currently not known. Here, we present nonlinear optical studies showing that lithium nickel manganese cobalt oxide nanosheets carrying a negative ζ-potential have no discernible consequences for lipid alignment and interleaflet composition in supported lipid bilayers formed from zwitterionic and negatively charged lipids. In contrast, lithiated and delithiated LiCoO2 nanosheets having positive and neutral ζ-potentials, respectively, alter the compositional asymmetry of the two membrane leaflets, and bilayer asymmetry remains disturbed even after rinsing. The insight that some cobalt oxide nanoformulations induce alterations to the compositional asymmetry in idealized model membranes may represent an important step toward assessing the biological consequences of their predicted widespread use.
authors.affiliation: Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Merve
authors.initials: M
authors.lastname: Doğangün
authors.forename: Mimi N
authors.initials: MN
authors.lastname: Hang
authors.affiliation: Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Julianne M
authors.initials: JM
authors.lastname: Troiano
authors.affiliation: Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Alicia C
authors.initials: AC
authors.lastname: McGeachy
authors.forename: Eric S
authors.initials: ES
authors.lastname: Melby
authors.forename: Joel A
authors.initials: JA
authors.lastname: Pedersen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Hamers
authors.affiliation: Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Franz M
authors.initials: FM
authors.lastname: Geiger
mesh_terms: D002462:Cell Membrane / Q000187:drug effects*; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000737:chemistry; D008564:Membrane Potentials / Q000187:drug effects; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D008051:Lipid Bilayers; D010087:Oxides; C491691:lithium cobalt oxide; D003035:Cobalt
keywords: nano−bio interface; phospholipid bilayer asymmetry; secondary ion mass spectrometry; silica/water interfaces; sum-frequency generation; transbilayer movement