title: [Effect of Bushen Huoxue Compound on Retinal Müller Cells in High Glucose or AGEs Conditions].
abstract: OBJECTIVE
  To explore the effect of Bushen Huoxue Compound (BHC) on lactate dehydrogenase (LDH) leakage, expressions of vascular endothelial growth factor (VEGF) and VEGF mRNA in retinal Muller cells under high glucose condition or advanced glycosylation end products (AGEs) condition by using serum pharmacological method.
  
  
  METHODS
  The retinal Müller cells of 5-7 days post-natal Sprague Dawley (SD) rats were cultured with modified enzyme-digestion method. Purified retinal Muller cells were cultured in normal conditions, high glucose condition (50 mmol/L) or AGEs (50 mg/L and 100 mg/L) conditions, and BHC-containing serum was added to culture medium. The LDH leakage and VEGF expressions were measured by enzyme-linked immunosorbent assay (ELISA). In addition, the relative expression of VEGF mRNA was tested by reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Compared with the normal control group, expressions of VEGF and VEGF mRNA were significantly increased in the high glucose group, the low dose AGEs group and the high dose AGEs group (all P < 0.01). The LDH leakage was obviously increased in the high dose AGEs group, when compared with the normal control group and the high glucose group (P < 0.01). The LDH leakage, expressions of VEGF and VEGF mRNA were obviously decreased by BHC-containing serum both in high glucose and AGEs conditions (P < 0.05, P < 0.01). BHC-containing serum had no significant effect on the LDH leakage and expressions of VEGF and VEGF mRNA in normal conditions (P > 0.05).
  
  
  CONCLUSIONS
  AGEs intervention could obviously lower the stability of Müller cell membrane. Up-regulated expressions of VEGF and VEGF mRNA in cultured Müller cells could be induced by AGEs or high glucose. BHC-containing serum could stabilize the stability of Müller cell membrane, inhibit the transcription of VEGF mRNA and decrease the protein expression of VEGF, which might be one of important mechanisms for preventing and treating diabetic retinopathy.
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Xie
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Song
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.forename: Yang
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003930:Diabetic Retinopathy; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D063928:Ependymoglial Cells; D005947:Glucose; D007770:L-Lactate Dehydrogenase; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C527851:bushen huoxue; D007770:L-Lactate Dehydrogenase; D005947:Glucose