title: Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations.
abstract: Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is a valuable source of genes for bread wheat (2n = 6x = 42) improvement because of its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines by backcrossing the amphiploid in the absence of the Ph1 gene (allowing intergenomic recombination) can assist its utilization in wheat improvement. In this study, six novel wheat-Th. bessarabicum translocation lines involving different chromosome segments (T4BS.4BL-4JL, T6BS.6BL-6JL, T5AS.5AL-5JL, T5DL.5DS-5JS, T2BS.2BL-2JL, and the whole arm translocation T1JS.1AL) were identified and characterized using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). No background translocations between wheat genomes were observed. The involvement of five of the seven chromosomes and small terminal segments of Th. bessarabicum chromosome arm were important, contributing to both reduced linkage drag of the derived lines by minimizing agronomically deleterious genes from the alien species and high stability including transmission of the alien segment. All three wheat genomes were involved in the translocations with the alien chromosome, and GISH showed the Th. bessarabicum genome was more closely related to the D genome in wheat. All the introgression lines were disomic, stable, and with good morphological characters.
authors.affiliation: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. chetan.patokar@gmail.com.
authors.forename: Chetan
authors.initials: C
authors.lastname: Patokar
authors.affiliation: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. phh4@le.ac.uk.
authors.forename: Adel
authors.initials: A
authors.lastname: Sepsi
authors.affiliation: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. ts32@le.ac.uk.
authors.forename: Trude
authors.initials: T
authors.lastname: Schwarzacher
authors.affiliation: International Maize and Wheat Improvement Center (CIMMYT), Km. 45, Carretera, México-Veracruz El Batán, CP 56237, Texcoco, Edo. de México, Mexico. M.Kishii@cgiar.org.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kishii
authors.affiliation: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. ais7@le.ac.uk.
authors.forename: J S
authors.initials: JS
authors.lastname: Heslop-Harrison
mesh_terms: D032461:Chromosomes, Plant; D020732:Cytogenetic Analysis; D022761:Gene Transfer, Horizontal; D017403:In Situ Hybridization; D007178:Inbreeding; D006109:Poaceae / Q000235:genetics*; D014178:Translocation, Genetic