title: Structural lipid changes and Na(+)/K(+)-ATPase activity of gill cells' basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles.
abstract: Seawater acclimation is a critical period for anadromous species and a process yet to be understood in lampreys. Considering that changes in lipid composition of the gill cells' basolateral membranes may disrupt the major transporter Na(+)K(+)-ATPase, the goal of this study was to detect changes at this level during juvenile sea lamprey seawater acclimation. The results showed that saltwater acclimation has a direct effect on the fatty acid composition of gill cells basolateral membrane's phospholipids. When held in full-strength seawater, the fatty acid profile of basolateral membrane's phospholipids suffered a restructure by increasing either saturation or the ratio between oleic acid and eicosapentaenoic acid. Simultaneously, the activity of Na(+)K(+)-ATPase revealed a significant and positive correlation with basolateral membrane's cholesterol content in the presence of highest salinity. Our results pointed out for lipid adjustments involving the functional transporter present on the gill cell basolateral membranes to ensure the role played by branchial Na(+)K(+)-ATPase in ion transport during saltwater acclimation process. The responses observed contributed to the strategy adopted by gill cell's basolateral membranes to compensate for osmotic and ionic stressors, to ensure the success of the process of seawater acclimation associated with the downstream trophic migration of juvenile sea lamprey.
authors.affiliation: Departamento de Zootecnia, Escola de Ciências e Tecnologia, ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Largo dos Colegiais 2, Évora 7004-516, Portugal. Electronic address: mjlanca@uevora.pt.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Lança
authors.affiliation: Hospital Veterinário das Laranjeiras, Rua São Tomás de Aquino 8C, Lisboa 100-203, Portugal. Electronic address: maria.potes.amaral.machado@gmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Machado
authors.affiliation: MARE-Centro de Ciências do Mar e do Ambiente, Departamento de Biologia, Escola de Ciências e Tecnologia, Universidade de Évora, Largo dos Colegiais 2, Évora 7004-516, Portugal. Electronic address: afferreira@fc.ul.pt.
authors.forename: Ana Filipa
authors.initials: AF
authors.lastname: Ferreira
authors.affiliation: MARE-Centro de Ciências do Mar e do Ambiente, Departamento de Biologia, Escola de Ciências e Tecnologia, Universidade de Évora, Largo dos Colegiais 2, Évora 7004-516, Portugal; Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, Lisboa 1749-016, Portugal. Electronic address: bsquintella@fc.ul.pt.
authors.forename: Bernardo Ruivo
authors.initials: BR
authors.lastname: Quintella
authors.affiliation: MARE-Centro de Ciências do Mar e do Ambiente, Departamento de Biologia, Escola de Ciências e Tecnologia, Universidade de Évora, Largo dos Colegiais 2, Évora 7004-516, Portugal. Electronic address: pmra@uevora.pt.
authors.forename: Pedro Raposo
authors.initials: PR
authors.lastname: de Almeida
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005618:Fresh Water; D005880:Gills / Q000166:cytology / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D009994:Osmolar Concentration; D064587:Osmoregulation / Q000502:physiology; D048428:Petromyzon / Q000378:metabolism / Q000502:physiology*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D012623:Seawater; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Basolateral membrane; Gill cells; Juveniles; Petromyzon marinus; Phospholipids' composition; Saltwater acclimation