title: Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01.
abstract: Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes' activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
authors.forename: Pei-jun
authors.initials: PJ
authors.lastname: Li
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China. jlxia@csu.edu.cn.
authors.forename: Jin-lan
authors.initials: JL
authors.lastname: Xia
authors.affiliation: Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shan
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
authors.forename: Zhen-yuan
authors.initials: ZY
authors.lastname: Nie
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development / Q000528:radiation effects; D018556:Crops, Agricultural / Q000382:microbiology* / Q000528:radiation effects; D005285:Fermentation / Q000502:physiology; D007220:Industrial Waste / Q000517:prevention & control*; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000528:radiation effects; D013010:Sonication / Q000379:methods*
chemical_list: D007220:Industrial Waste; D009097:Multienzyme Complexes
keywords: Multi-enzyme complexes; Solid-state fermentation; Submerged fermentation; Ultrasound; Zymography