title: Human Toll like receptor 4 gene expression of PBMCs in diabetes mellitus type 2 patients.
abstract: Toll like receptor 4 (TLR4) is one of the most pivotal pathogen recognition receptors (PRRs) in innate immune systems. In this study, we evaluate the expression of the TLR4 in patients with diabetes mellitus type 2 (DM2) in comparison to healthy controls. Expression of TLR4 in 32 human peripheral blood mononuclear cells (PBMCs) of patients with DM2 and 20 control samples was assessed using Real&mdash;Time PCR technique. For each patient, body mass index (BMI) and blood glucose levels were measured. The results of Real&mdash;Time PCR showed a 5&mdash;folds increase in expression of TLR4 on the PBMCs of DM2 patients in comparison to controls. No correlation was observed between the TLR4 expression and sex or BMI. Our results confirmed that DM2 can increase TLR4 expression independent from sex, blood glucose concentrations and BMI.
authors.affiliation: Zabol University of Medical Sciences Zabol Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Sepehri
authors.affiliation: Kerman University of Medical Sciences Kerman Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Kiani
authors.affiliation: Zabol University of Medical Sciences Zabol Iran.
authors.forename: A A
authors.initials: AA
authors.lastname: Nasiri
authors.affiliation: Zahedan University of Medical Sciences Zahedan Iran.
authors.forename: M A
authors.initials: MA
authors.lastname: Mashhadi
authors.affiliation: Zabol University of Medical Sciences Zabol Medicinal Plant Research Center Zabol Iran fer.javadian&commat;gmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Javadian
authors.affiliation: Harvard Medical School Department of Genetics Boston USA.
authors.forename: A
authors.initials: A
authors.lastname: Haghighi
authors.affiliation: Zabol University of Medical Sciences Zabol Iran.
authors.forename: F
authors.initials: F
authors.lastname: Kohan
authors.affiliation: University of Zanjan Research Institute of Modern Biological Techniques Zanjan Iran.
authors.forename: A
authors.initials: A
authors.lastname: Bahari
authors.affiliation: Zabol University of Medical Sciences Zabol Iran.
authors.forename: A
authors.initials: A
authors.lastname: Sargazi
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4