title: Immunoprecipitation of Cdk-Cyclin Complexes for Determination of Kinase Activity.
abstract: Cyclin-dependent kinases (Cdks) belong to a family of key regulators of cell division cycle and transcription. The activity of some of them is deregulated in tumor cells and to find specific inhibitors is an important goal to be achieved. We report here the current methods to determine their in vitro activity in order to facilitate the identification of specific inhibitors. Mainly, the activity can be determined by using immunoprecipitates from cell samples with antibodies against specific Cdks as a source of the enzymes.
authors.affiliation: Department of Cell Biology, Immunology and Neurosciences, University of Barcelona, Barcelona, Spain.
authors.forename: Edurne
authors.initials: E
authors.lastname: Gallastegui
authors.forename: Oriol
authors.initials: O
authors.lastname: Bachs
mesh_terms: D000818:Animals; D002021:Buffers; D002453:Cell Cycle / Q000235:genetics*; D004396:Coloring Agents / Q000737:chemistry; D018844:Cyclin-Dependent Kinases / Q000302:isolation & purification* / Q000378:metabolism; D016213:Cyclins / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D051379:Mice; D008967:Molecular Biology / Q000379:methods; D011817:Rabbits; D051381:Rats; D012756:Sheep
chemical_list: D002021:Buffers; D004396:Coloring Agents; D016213:Cyclins; D018844:Cyclin-Dependent Kinases