title: Comparison of conjunctival staining between lissamine green and yellow filtered fluorescein sodium.
abstract: OBJECTIVE
  To compare conjunctival staining between lissamine green and fluorescein sodium using a yellow barrier filter.
  
  
  METHODS
  Retrospective cross-sectional study.
  
  
  METHODS
  Thirteen eyes of 13 patients with dry eyes.
  
  
  METHODS
  All patients underwent ocular surface staining with fluorescein sodium and lissamine green. Ocular surface staining with fluorescein sodium was observed through slit-lamp examination under cobalt blue illumination and a yellow filter. Temporal (0-5) and nasal (0-5) conjunctival staining grade was separately graded according to the Oxford scheme. The contrast score was defined as the absolute difference between mean grey values of punctate staining and background. Conjunctival staining grade and contrast scores were compared between these 2 staining methods.
  
  
  RESULTS
  The sum of the conjunctival staining grade (0-10) for the nasal and temporal conjunctiva was 4.7 ± 2.5 for fluorescein sodium, which was significantly higher than that of lissamine green, 4.1 ± 2.5 (p = 0.005). The contrast score of temporal and nasal conjunctival staining was 34.6 ± 12.0 and 34.7 ± 10.3, respectively, for fluorescein sodium, which was also significantly greater than for lissamine green (23.4 ± 8.1 and 21.0 ± 9.0, respectively; p = 0.003 and p = 0.003, respectively).
  
  
  CONCLUSIONS
  Conjunctival staining with fluorescein sodium and a yellow filter seems to be more sensitive for the detection of conjunctival damage than lissamine green. Fluorescein staining with the yellow filter has the advantage of simultaneous observation of both corneal and conjunctival damage in patients with dry eye without the need for additional vital staining.
authors.affiliation: Department of Ophthalmology, Korea University College of Medicine.
authors.forename: Youngsub
authors.initials: Y
authors.lastname: Eom
authors.affiliation: Department of Ophthalmology, Korea University College of Medicine.
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Hyung
authors.initials: H
authors.lastname: Keun Lee
authors.affiliation: Department of Ophthalmology, Korea University College of Medicine.
authors.forename: Hyo
authors.initials: H
authors.lastname: Myung Kim
authors.affiliation: Department of Ophthalmology, Korea University College of Medicine. Electronic address: crisim@korea.ac.kr.
authors.forename: Jong
authors.initials: J
authors.lastname: Suk Song
mesh_terms: D000328:Adult; D004396:Coloring Agents / Q000008:administration & dosage* / Q000737:chemistry; D003228:Conjunctiva / Q000473:pathology*; D003430:Cross-Sectional Studies; D015352:Dry Eye Syndromes / Q000175:diagnosis*; D005260:Female; D019793:Fluorescein / Q000008:administration & dosage* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008086:Lissamine Green Dyes / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013194:Staining and Labeling / Q000379:methods; D055815:Young Adult
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D008086:Lissamine Green Dyes; D019793:Fluorescein