title: Adipose-derived stem cell transplantation for therapeutic lymphangiogenesis in a mouse secondary lymphedema model.
abstract: OBJECTIVE
  Secondary lymphedema is observed in common after postmalignancy treatment of the breast and the gynecologic organs but effective therapies are not established. Adipose-derived stem cells (ADSCs), which are pluripotent, regenerative in local injection, are tested for murine hindlimb secondary lymphedema by regenerative method.
  
  
  RESULTS
  Mice were divided into four groups: no ADSCs, 1 × 10(6) ADSCs, 1 × 10(5) ADSCs and 1 × 10(4) ADSCs (each group, n = 20) in a stringent surgical resection and irradiation. Circumferential measurement, lymphatic flow assessment and quantification of lymphatic vessels were performed.
  
  
  RESULTS
  The numbers of lymphatic vessels by LYVE-1 immunohistochemistry, and VEGF-C- or VEGFR3-expressing cells were significantly increased in transplanted groups (p < 0.05).
  
  
  CONCLUSIONS
  ADSCs can restore the lymphatic vascular network in secondary lymphedema with increased collecting vessels.
authors.affiliation: Division of Plastic & Reconstructive Surgery, Department of Developmental & Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852 8501, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Division of Plastic & Reconstructive Surgery, Department of Developmental & Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852 8501, Japan.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Hamuy
authors.affiliation: Division of Plastic & Reconstructive Surgery, Oita Sanai Medical Center, 1213 Ichi, Oita 870-1151, Japan.
authors.forename: Yuuichi
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: Division of Plastic & Reconstructive Surgery, Department of Developmental & Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852 8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshimoto
authors.affiliation: Division of Plastic & Reconstructive Surgery, Department of Developmental & Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852 8501, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hirano
authors.affiliation: Division of Plastic & Reconstructive Surgery, Department of Developmental & Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852 8501, Japan.
authors.forename: Sadanori
authors.initials: S
authors.lastname: Akita
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006614:Hindlimb / Q000473:pathology; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007259:Infrared Rays; D042583:Lymphangiogenesis / Q000502:physiology*; D042601:Lymphatic Vessels / Q000378:metabolism; D008209:Lymphedema / Q000628:therapy*; D008297:Male; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D012038:Regeneration; D033581:Stem Cell Transplantation; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism; D014741:Video Recording
chemical_list: D006023:Glycoproteins; D049452:Green Fluorescent Proteins; D026901:Membrane Transport Proteins; D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3; C492273:LYVE1 protein, mouse
keywords: VEGF-C; VEGFR3; adipose-derived stem cell; lymphangiogenesis; mouse; radiation; secondary lymphedema; surgery