title: Introducing differential expression of human heat shock protein 27 in hepatocellular carcinoma: moving toward identification of cancer biomarker.
abstract: Previously, it has to be acknowledged that overexpressed heat shock protein B27 (HSPB27) have been implicated in the etiology of wide range of human cancers. However, the molecular mechanism leading to the disease initiation to progression in liver cancer is still unknown. Present work was undertaken to investigate the differentially expressed HSPB27 in association with those damages that lead to liver cancer development. For the identification of liver cancer biomarker, samples were subjected to comparative proteomic analysis using two-dimensional gel electrophoresis (2-DE) and were further validated by Western blot and immunohistochemical analysis. After validation, in silico studies were applied to demonstrate the significantly induced phosphorylated and S-nitrosylated signals. The later included the interacting partner of HSPB27, i.e., mitogen-activated protein kinase-3 and 5 (MAPK3 and 5), ubiquitin C (UBC), v-akt murine thymoma viral oncogene homolog 1 (AKT1), mitogen-activated protein kinase 14 (MAPK14), and tumor protein p53 (TP53), which bestowed with critical capabilities, namely, apoptosis, cell cycling, stress activation, tumor suppression, cell survival, angiogenesis, proliferation, and stress resistance. Taking together, these results shed new light on the potential biomarker HSPB27 that overexpression of HSPB27 did lead to upregulation of their interacting partner that together demonstrate their possible role as a novel tumor progressive agent for the treatment of metastasis in liver cancer. HSPB27 is a promising diagnostic marker for liver cancer although further large-scale studies are required. Also, molecular profiling may help pave the road to the discovery of new therapies.
authors.affiliation: Department of Biochemistry, Ziauddin University, Clifton, Karachi, Pakistan. rizmakhan@hotmail.com.|Department of Molecular Genetics, Dr. Ziauddin Hospital, North Nazimabad, Karachi, Pakistan. rizmakhan@hotmail.com.|Department of Biochemistry, University of Karachi-Pakistan, Karachi, Pakistan. rizmakhan@hotmail.com.
authors.forename: Rizma
authors.initials: R
authors.lastname: Khan
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Nadir Naveed
authors.initials: NN
authors.lastname: Siddiqui
authors.affiliation: Department of Biochemistry, University of Karachi-Pakistan, Karachi, Pakistan.
authors.forename: Ahtesham
authors.initials: A
authors.lastname: Ul Haq
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: M Ataur
authors.initials: MA
authors.lastname: Rahman
mesh_terms: D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins
keywords: Heat shock protein B27; Hepatitis C virus; Two-dimensional gel electrophoresis; Western blotting