title: Measurement of oxidative stress in the follicular fluid of infertility patients with an endometrioma.
abstract: OBJECTIVE
  Follicular fluid (FF) might reflect the environment during follicle and oocyte growth, and an evaluation of oxidative stress in the FF might be useful in predicting oocyte quality. In order to measure the oxidative stress (OS) in the FF from a single follicle of patients with endometrioma (EM), we evaluated whether an EM might affect the environment of follicular growth.
  
  
  METHODS
  Between December 2011 and July 2013, 26 patients with a unilateral EM (EM group) and 29 without EM (control group) were enrolled in this study. The FF was obtained during the first puncture of follicular aspiration, and was stored at -30 °C until it was assayed. A Free Radical Elective Evaluator (WISMERLL, USA) was used to perform d-ROM and BAP tests to measure oxidative stress (U.CARR) and antioxidant power (μmol/L).
  
  
  RESULTS
  The d-ROM values in the EMC and control groups were 328.7 ± 97.8 and 414.9 ± 84.2, respectively, and the BAP values for the two groups were 2474.3 ± 432.0 and 2552.8 ± 435.58, respectively. These values were similar between the two groups (mean ± SD). The number of patients with a modified BAP/d-ROM ratio of <1.0 in the EM group was similar to that for the control group at 16 and 15, respectively (61.5 and 51.7 %).
  
  
  CONCLUSIONS
  The oxidative stress and antioxidant potential in the FF of the patients with unilateral EM showed values similar to those without an EM. Therefore, we concluded that EMs do not affect the environment for follicle growth during ART treatment.
authors.affiliation: Division of Reproductive Medicine, Sugiyama Clinic, 1-53-1, Ohara, Setagaya, Tokyo, 156-0041, Japan. nakagawa-jiko@spice.ocn.ne.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: Department of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo, Japan.
authors.forename: Michi
authors.initials: M
authors.lastname: Hisano
authors.affiliation: Division of Reproductive Medicine, Sugiyama Clinic, 1-53-1, Ohara, Setagaya, Tokyo, 156-0041, Japan.
authors.forename: Rikikazu
authors.initials: R
authors.lastname: Sugiyama
authors.affiliation: Department of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo, Japan.
authors.forename: Koushi
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004715:Endometriosis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000097:blood; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006062:Gonadotropins / Q000097:blood; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology / Q000628:therapy; D008297:Male; D009865:Oocytes / Q000378:metabolism / Q000473:pathology; D006080:Ovarian Follicle / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D018384:Oxidative Stress; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D006062:Gonadotropins; D004958:Estradiol
keywords: Antioxidant potential; BAP; Endometrioma; Follicular fluid; Oxidative stress; d-ROM