title: A novel multitarget tracking algorithm for Myosin VI protein molecules on actin filaments in TIRFM sequences.
abstract: We propose a novel multitarget tracking framework for Myosin VI protein molecules in total internal reflection fluorescence microscopy sequences which integrates an extended Hungarian algorithm with an interacting multiple model filter. The extended Hungarian algorithm, which is a linear assignment problem based method, helps to solve measurement assignment and spot association problems commonly encountered when dealing with multiple targets, although a two-motion model interacting multiple model filter increases the tracking accuracy by modelling the nonlinear dynamics of Myosin VI protein molecules on actin filaments. The evaluation of our tracking framework is conducted on both real and synthetic total internal reflection fluorescence microscopy sequences. The results show that the framework achieves higher tracking accuracies compared to the state-of-the-art tracking methods, especially for sequences with high spot density.
authors.affiliation: Department of Computer Science, University of Warwick, UK.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Computer Science, University of Warwick, UK.
authors.forename: V
authors.initials: V
authors.lastname: Sanchez
authors.affiliation: Department of Computer Science, University of Warwick, UK.
authors.forename: P C S B
authors.initials: PC
authors.lastname: Nagaraj
authors.affiliation: Department of Biology, Lahore University of Management Sciences (LUMS), Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.affiliation: Department of Computer Science, University of Warwick, UK.|Department of Computer Science & Engineering (CSE), Qatar University, Qatar.
authors.forename: N
authors.initials: N
authors.lastname: Rajpoot
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry*; D000465:Algorithms; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D018995:Myosin Heavy Chains / Q000032:analysis*; D061848:Optical Imaging / Q000379:methods*
chemical_list: C089785:myosin VI; D018995:Myosin Heavy Chains
keywords: IMM filter; Kalman filter; linear assignment problem; motion model; multitarget tracking