title: Effect of Three-spot Seahorse Petroleum Ether Extract on Lipopolysaccharide Induced Macrophage RAW264.7 Inflammatory Cytokine Nitric Oxide and Composition Analysis.
abstract: Three-Spot seahorse is a traditional medicine in Asian countries. However, the alcohol extract is largely unknown for its anti-inflammatory activity. This study aimed at elucidating fraction of potent anti-inflammatory activity of seahorse. A systematic solvent extraction method of liquid-liquid fractionation of ethanol crude extract gave four fractions petroleum ether (PE), and ethyl acetate (EtOAc), water saturated butanol (n-BuOH), water (H2O). In this study, PE extract was selected for further study after preliminary screening test, and was connected to silica column chromatography and eluted with different polarity of mobile phases, and obtained four active fractions (Fr I, Fr II, Fr III, Fr IV). Effect of separated fractions on inflammation was investigated in lipopolysaccharide (LPS) stimulated murine RAW264.7 cells in vitro. The result shows that seahorse extract was capable of inhibiting the production of nitric oxide (NO) significantly in a dose dependent manner and exhibited no notable cytotoxicity on cell viability. IC50 of fraction IV was 36.31 μg/mL, indicating that separated fraction possessed potent NO inhibitory activity against LPS-induced inflammatory response, thus, demonstrated its in vitro anti-inflammatory potentiality, it may be at least partially explained by the presence of anti-inflammation active substances, phenolic compounds, phospholipids and polyunsaturated fatty acids, especially phospholipids and polyunsaturated fatty acids. It could be suggested that seahorse lipid-soluble components could be used in functional food and anti-inflammatory drug preparations.
authors.affiliation: Laboratory of Aquatic Product Processing and Storage, College of Food Science and Technology, Hainan University.
authors.forename: LiPing
authors.initials: L
authors.lastname: Chen
authors.forename: XuanRi
authors.initials: X
authors.lastname: Shen
authors.forename: GuoHua
authors.initials: G
authors.lastname: Chen
authors.forename: XianYing
authors.initials: X
authors.lastname: Cao
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000473:Alkanes; D000818:Animals; D000893:Anti-Inflammatory Agents; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D018836:Inflammation Mediators / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D000067996:RAW 264.7 Cells; D023701:Smegmamorpha; D014020:Tissue Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000473:Alkanes; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005231:Fatty Acids, Unsaturated; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010743:Phospholipids; D014020:Tissue Extracts; D009569:Nitric Oxide; C004544:naphtha