title: A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari.
abstract: In this study, we devised a multiplex PCR assay based on the gene of cytolethal distending toxin (cdt) B subunit to simultaneously detect and discriminate Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari. Species-specific PCR products were successfully obtained from all 38 C. jejuni, 12 C. fetus, 39 C. coli, 22 C. upsaliensis, 24 C. hyointestinalis, and 7 C. lari strains tested. On the other hand, no specific PCR products were obtained from other campylobacters and bacterial species tested (41 strains in total). The proposed multiplex PCR assay is a valuable tool for detection and descrimination of 6 major Campylobacter species, that are associated with gastrointestinal diseases in humans.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Kamei
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kawabata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asakura
authors.forename: Worada
authors.initials: W
authors.lastname: Samosornsuk
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hinenoya
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics; D002169:Campylobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D017000:Campylobacter coli / Q000235:genetics* / Q000302:isolation & purification; D002168:Campylobacter fetus / Q000235:genetics* / Q000302:isolation & purification; D044862:Campylobacter hyointestinalis / Q000235:genetics* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D044863:Campylobacter lari / Q000235:genetics* / Q000302:isolation & purification; D044885:Campylobacter upsaliensis / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003937:Diagnosis, Differential; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; C067044:cytolethal distending toxin