title: A Catalytic DNA Probe with Stem-loop Motif for Human T47D Breast Cancer Cells.
abstract: In vitro selection methods allow for isolation of DNAzymes (catalytic DNAs) from random DNA pools. Here we describe a fluorogenic DNAzyme, LYF5, isolated using a double-random selection approach: a random DNA pool was selected against a complex molecular mixture derived from a breast cancer cell line, T47D. LYF5 specifically indicates the T47D breast cancer cell line with high sensitivity. After sequence optimization, the second-generation DNAzyme, 2G-LYF5, exhibited an approximately 2-fold higher cleavage percentage. Finally, we have determined that the intramolecular stem-loop motif plays a crucial role in 2G-LYF5 activity. Our findings underscore the capability of single-stranded DNA molecules to perform highly sophisticated functions that are amenable to the development of diagnostic tests for early identification of breast cancer.
authors.affiliation: Department of Chemistry, Tsinghua University.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: MeiYun
authors.initials: M
authors.lastname: Zeng
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000235:genetics* / Q000378:metabolism*; D021881:DNA, Catalytic / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D055029:Inverted Repeat Sequences; D008969:Molecular Sequence Data
chemical_list: D015342:DNA Probes; D021881:DNA, Catalytic