title: Effects of arachidonic acid intake on inflammatory reactions in dextran sodium sulphate-induced colitis in rats.
abstract: The aim of this study was to investigate the effects of the administration of oral arachidonic acid (AA) in rats with or without dextran sulphate sodium (DSS)-induced inflammatory bowel disease. Male Wistar rats were administered AA at 0, 5, 35 or 240 mg/kg daily by gavage for 8 weeks. Inflammatory bowel disease was induced by replacing drinking water with 3 % DSS solution during the last 7 d of the AA dosing period. These animals passed loose stools, diarrhoea and red-stained faeces. Cyclo-oxygenase-2 concentration and myeloperoxidase activity in the colonic tissue were significantly increased in the animals given AA at 240 mg/kg compared with the animals given AA at 0 mg/kg. Thromboxane B2 concentration in the medium of cultured colonic mucosae isolated from these groups was found to be dose-dependently increased by AA, and the increase was significant at 35 and 240 mg/kg. Leukotriene B4 concentration was also significantly increased and saturated at 5 mg/kg. In addition, AA at 240 mg/kg promoted DSS-induced colonic mucosal oedema with macrophage infiltration. In contrast, administration of AA for 8 weeks, even at 240 mg/kg, showed no effects on the normal rats. These results suggest that in rats with bowel disease AA metabolism is affected by oral AA, even at 5 mg/kg per d, and that excessive AA may aggravate inflammation, whereas AA shows no effects in rats without inflammatory bowel disease.
authors.affiliation: 1Department of Genomic Medicine,National Cerebral and Cardiovascular Center,Suita,Osaka 565-8565,Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Naito
authors.affiliation: 1Department of Genomic Medicine,National Cerebral and Cardiovascular Center,Suita,Osaka 565-8565,Japan.
authors.forename: Xu
authors.initials: X
authors.lastname: Ji
authors.affiliation: 4Hatano Research Institute,Food and Drug Safety Center,Hadano,Kanagawa 257-8523,Japan.
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Tachibana
authors.affiliation: 4Hatano Research Institute,Food and Drug Safety Center,Hadano,Kanagawa 257-8523,Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Aoki
authors.affiliation: 4Hatano Research Institute,Food and Drug Safety Center,Hadano,Kanagawa 257-8523,Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Furuya
authors.affiliation: 4Hatano Research Institute,Food and Drug Safety Center,Hadano,Kanagawa 257-8523,Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tazura
authors.affiliation: 5College of Pharmacy,Kinjo Gakuin University,Nagoya,Aichi 463-8521,Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miyazawa
authors.affiliation: 6Department of Food and Life Science,School of Life and Environmental Science,Azabu University,Sagamihara,Kanagawa 252-0206,Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Harauma
authors.affiliation: 6Department of Food and Life Science,School of Life and Environmental Science,Azabu University,Sagamihara,Kanagawa 252-0206,Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Moriguchi
authors.affiliation: 4Hatano Research Institute,Food and Drug Safety Center,Hadano,Kanagawa 257-8523,Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagata
authors.affiliation: 1Department of Genomic Medicine,National Cerebral and Cardiovascular Center,Suita,Osaka 565-8565,Japan.
authors.forename: Naoharu
authors.initials: N
authors.lastname: Iwai
authors.affiliation: 5College of Pharmacy,Kinjo Gakuin University,Nagoya,Aichi 463-8521,Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohara
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000009:adverse effects* / Q000378:metabolism; D003092:Colitis / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016264:Dextran Sulfate; D004032:Diet; D007249:Inflammation / Q000378:metabolism*; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007975:Leukotriene B4 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism*; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000378:metabolism
chemical_list: D007975:Leukotriene B4; D016718:Arachidonic Acid; D013929:Thromboxane B2; D016264:Dextran Sulfate; D009195:Peroxidase; D051546:Cyclooxygenase 2
keywords: 6-keto PGF1α 6-keto prostaglandin F1α; AA arachidonic acid; Arachidonic acid; COX-2 cyclo-oxygenase-2; Colitis; DSS dextran sulphate sodium; Dextran sodium sulphate; LTB4 leukotriene B4; MPO myeloperoxidase; PGE2 prostaglandin E2; Supplementation; TXB2 thromboxane B2