title: Dependence of the bystander effect for micronucleus formation on dose of heavy-ion radiation in normal human fibroblasts.
abstract: Ionising radiation-induced bystander effects are well recognised, but its dependence on dose or linear energy transfer (LET) is still a matter of debate. To test this, 49 sites in confluent cultures of AG01522D normal human fibroblasts were targeted with microbeams of carbon (103 keV µm(-1)), neon (375 keV µm(-1)) and argon ions (1260 keV µm(-1)) and evaluated for the bystander-induced formation of micronucleus that is a kind of a chromosome aberration. Targeted exposure to neon and argon ions significantly increased the micronucleus frequency in bystander cells to the similar extent irrespective of the particle numbers per site of 1-6. In contrast, the bystander micronucleus frequency increased with increasing the number of carbon-ion particles in a range between 1 and 3 particles per site and was similar in a range between 3 and 8 particles per site. These results suggest that the bystander effect of heavy ions for micronucleus formation depends on dose.
authors.affiliation: Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan Present Address: Radiation Oncology, Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8576, Japan ymatsumoto@pmrc.tsukuba.ac.jp.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Radiation Safety Research Center, Nuclear Technology Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI), 2-11-1 Iwado-kita, Komae, Tokyo 201-8511, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamada
authors.affiliation: Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Aoki-Nakano
authors.affiliation: Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292, Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Funayama
authors.affiliation: Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakashita
authors.affiliation: Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences, Higashi 23-35-1, Towada, Aomori 034-8628, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Wada
authors.affiliation: Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences, Higashi 23-35-1, Towada, Aomori 034-8628, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kakizaki
authors.affiliation: Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Furusawa
mesh_terms: D024201:Bystander Effect / Q000528:radiation effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D020450:Heavy Ions / Q000009:adverse effects*; D006801:Humans; D018499:Linear Energy Transfer / Q000528:radiation effects; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D011829:Radiation Dosage