title: Long noncoding RNA NEAT1 promotes glioma pathogenesis by regulating miR-449b-5p/c-Met axis.
abstract: Growing evidence demonstrates that long noncoding RNAs (lncRNAs) are involved in the progression of various cancers including glioma. Nuclear enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, was not fully explored in glioma. We aimed to determine the expression, roles, and functional mechanisms of NEAT1 in the progression of glioma. By real-time PCR, we suggested that NEAT1 was upregulated in glioma tissues than noncancerous brain tissues. Knockdown of NEAT1 reduced glioma cell proliferation, invasion, and migration. RNA immunoprecipitation assay combined with luciferase reporter assay confirmed miR-449b-5p-specific binding to NEAT1. Furthermore, we verified that c-Met was a directly target of miR-449b-5p. Rescue assays demonstrated NEAT1 functions a molecular sponge for miR-449b-5p and leads to the upregulation of c-Met. This regulation menchaism promotes glioma pathogenesis and may provide a potential target for the prognosis and treatment of glioma.
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Sanhao Street, Shenyang, Liaoning province, 110004, People's Republic of China. lizhen7111@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhen
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Sanhao Street, Shenyang, Liaoning province, 110004, People's Republic of China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yun-Hui
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Sanhao Street, Shenyang, Liaoning province, 110004, People's Republic of China.
authors.forename: Diao
authors.initials: D
authors.lastname: Hong-Yu
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning province, 110001, People's Republic of China.
authors.forename: Ma
authors.initials: M
authors.lastname: Jun
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Sanhao Street, Shenyang, Liaoning province, 110004, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi-Long
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008954:Models, Biological; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics*; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C544530:MIRN449 microRNA, human; D035683:MicroRNAs; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D019859:Proto-Oncogene Proteins c-met
keywords: Glioma; Long noncoding RNA; NEAT1; c-Met; miR-449b-3p