title: Effects of chronic and subtoxic chlorobenzenes on adrenocorticotrophic hormone release.
abstract: Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentially important area of research to develop in vivo and in vitro experimental models. In this context, the primary aim of this study was to demonstrate the effects of chlorobenzenes on adrenocorticotrophic hormone (ACTH) release. In our experimental study, male Wistar rats were exposed to 0.1, 1.0 and 10 μg/b.w. (body weight)kg of 1,2,4- trichlorobenzene and hexachlorobenzene (ClB) mix via gastric tube for 30, 60 or 90 days. At the endpoints of the experiment blood samples were taken and animals were decapitated. Primary, monolayer adenohypophysis cell cultures were prepared by enzymatic and mechanical digestion. The ACTH hormone content in serum and supernatant media was measured by immuno-chemiluminescence assay. The Mg(2+)-dependent ATPase activity was determined by modified method of Martin and Dotty. Significant differences were detected in the hormone release between the control and treated groups. The hormone release was enhanced characteristically in exposed groups depending upon the dose and duration of exposure. The Mg(2+)-ATPase activity enhanced after chronic and subtoxic ClB exposition. Light microscopy revealed that the adenohypophysis seemed to be more abundant. Results indicate that Wistar rats exposed to subtoxic ClB have direct and indirect effects on hypothalamus-hypophysis-adrenal axis.
authors.affiliation: Institute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged H-6725, Hungary. Electronic address: molnar.zsolt@jgypk.u-szeged.hu.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Molnár
authors.affiliation: Department of Pulmonology, Faculty of Medicine, University of Szeged, Deszk H-6772, Hungary.
authors.forename: Regina
authors.initials: R
authors.lastname: Pálföldi
authors.affiliation: Department of Medical Physics and Informatics, Faculty of Medicine, University of Szeged, Szeged H-6725, Hungary.
authors.forename: Anna
authors.initials: A
authors.lastname: László
authors.affiliation: Institute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged H-6725, Hungary.
authors.forename: Marianna
authors.initials: M
authors.lastname: Radács
authors.affiliation: Endocrine Unit of First Department of Internal Medicine, Faculty of Medicine, University of Szeged, Szeged H-6725, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Sepp
authors.affiliation: Invasive Cardiology Department, Second Department of Internal Medicine and Cardiology, Faculty of Medicine, University of Szeged, Szeged H-6725, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Hausinger
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Szeged, Szeged H-6725, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Tiszlavicz
authors.affiliation: Endocrine Unit of First Department of Internal Medicine, Faculty of Medicine, University of Szeged, Szeged H-6725, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Valkusz
authors.affiliation: Institute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged H-6725, Hungary.
authors.forename: Márta
authors.initials: M
authors.lastname: Gálfi
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002722:Chlorobenzenes / Q000633:toxicity*; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000633:toxicity*; D006581:Hexachlorobenzene / Q000633:toxicity*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002722:Chlorobenzenes; D004785:Environmental Pollutants; C009947:1,2,4-trichlorobenzene; D006581:Hexachlorobenzene; D000324:Adrenocorticotropic Hormone
keywords: Adrenocorticotrophic hormone release; Chlorobenzenes; Mg(2+)-dependent ATPase; Subtoxic