title: Spectra, stability and labeling of 1-(5-carboxypentyl)-4-(2-(N-ethyl-carbazole-3-yl) vinyl) pyridinium bromide with a large Stokes shift.
abstract: Carbazole and its derivatives have been widely utilized as a functional building block in the fabrication of the organic medicine, pesticides, materials, etc., because of their excellent solubility, stability and biological activity. In this paper, 1-(5-carboxypentyl)-4-(2-(N-ethyl-carbazole-3-yl) vinyl) pyridinium bromide with a large Stokes shift was synthesized and characterized by (1)H NMR and MS. The UV/vis absorption and fluorescence spectra in different solvents and at different pH values were investigated preliminarily. The photostability and thermostability were also studied and the results showed that the compound was stable. The compound was also used to label bovine serum albumin (BSA) and calf thymus (ct)DNA. The results showed that the fluorescence intensity is enhanced when labeling with BSA and the binding ability is stronger than ctDNA, making it may be used as a biological probe.
authors.affiliation: School of Science, Tianjin Chengjian University, Tianjin, 300384, People's Republic of China.|Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Gu
authors.affiliation: School of Envirnmental Science and Engineering, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Lin
authors.affiliation: School of Science, Tianjin Chengjian University, Tianjin, 300384, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Science, Tianjin Chengjian University, Tianjin, 300384, People's Republic of China.|School of Envirnmental Science and Engineering, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Xuening
authors.initials: X
authors.lastname: Fei
authors.affiliation: School of Science, Tianjin Chengjian University, Tianjin, 300384, People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002227:Carbazoles / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D015394:Molecular Structure; D066244:Proton Magnetic Resonance Spectroscopy; D011726:Pyridinium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: C000613210:1-(5-carboxypentyl)-4-(2-(N-ethyl-carbazole-3-yl)vinyl)pyridinium bromide; D002227:Carbazoles; D011726:Pyridinium Compounds; D012710:Serum Albumin, Bovine; D004247:DNA; C427203:calf thymus DNA
keywords: carbazole; labeling; large Stokes shift; spectra; stability