title: Corallite-like Magnetic Fe3O4@MnO2@Pt Nanocomposites as Multiple Signal Amplifiers for the Detection of Carcinoembryonic Antigen.
abstract: A nonenzymatic sandwich-type electrochemical immunosensor using corallite-like magnetic Fe3O4@MnO2@Pt nanocomposites was developed for the sensitive detection of carcinoembryonic antigen (CEA). First, aminated graphene (GS-NH2) sheets were synthesized from graphite oxide using the Hummers' method, which was used to immobilize the primary antibody via the active amino groups on the GS-NH2. Second, corallite-like Fe3O4@MnO2@Pt nanoparticles (NPs) were synthesized and characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), and energy dispersive spectroscopy (EDS). They were used as labels to conjugate with a secondary antibody. The multiple amplification of Fe3O4@MnO2@Pt NPs and the promoted electron transfer of GS-NH2 lead to a broad linear range from 0.5 pg/mL to 20 ng/mL and a low detection limit with 0.16 pg/mL. In addition, the immunosensor performed with good selectivity and acceptable stability and reproducibility as well. The results are satisfactory when the proposed method has been applied to analyze human serum samples. Thus, there would be a promising future in the early diagnosis of cancer to detect CEA and other tumor markers.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Jia
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D017895:Manganese Compounds / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D017895:Manganese Compounds; D010087:Oxides; D010984:Platinum; C027424:manganese oxide; D006108:Graphite; D052203:Ferrosoferric Oxide
keywords: Fe3O4@MnO2@Pt nanoparticles; aminated graphene sheets; carcinoembryonic antigen; electrochemical immunosensor; signal amplification