title: Expression of nuclear membrane proteins in normal, hyperplastic, and neoplastic thyroid epithelial cells.
abstract: Emerin, lamin A/C, lamin B, and lamin-associated polypeptide 2 (LAP2) are nuclear membrane proteins that play an important role in maintaining nuclear structure and coordinating cell activity. We studied the expression and significance of nuclear membrane proteins in neoplastic thyroid cells by immunohistochemistry, RT-PCR, and real-time PCR. In papillary carcinomas (PCs), the nuclear proteins most frequently expressed at high levels were emerin (82 % positive), lamin A/C (64 %), and LAP2 (82 %). Follicular carcinomas (FCs) most frequently expressed lamin B, while none of the undifferentiated carcinomas (UCs) showed strong expression of emerin or lamin A/C. In all medullary carcinomas (MCs), intermediate to high levels of expression of lamin A/C and LAP2 were found. By RT-PCR analysis, messenger RNA (mRNA) expression of all nuclear membrane proteins except emerin was higher in PC than in normal tissue. Real-time PCR analysis showed that mRNA expression of nuclear membrane protein varied between cell lines. Our findings suggest that expression of nuclear membrane proteins may be related to follicular function in normal and hyperplastic follicles, and we hypothesize that they are also involved in the proliferation and differentiation of neoplastic thyroid cells. We suggest that they reflect the biological nature and/or function of normal, hyperplastic, and neoplastic thyroid cells and may have some value in diagnosing thyroid tumors.
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yamane
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Nakazawa
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Oish
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Mochizuki
authors.affiliation: Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan. rkatoh@yamanashi.ac.jp.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
mesh_terms: D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D004847:Epithelial Cells / Q000737:chemistry; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D034904:Lamin Type A / Q000032:analysis / Q000235:genetics; D034921:Lamin Type B / Q000032:analysis / Q000235:genetics; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013961:Thyroid Gland / Q000737:chemistry* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; D034904:Lamin Type A; D034921:Lamin Type B; D008565:Membrane Proteins; D009687:Nuclear Proteins; C092710:emerin; C440308:lamin C; C093413:lamina-associated polypeptide 2
keywords: Carcinoma; Immunohistochemistry; Nuclear membrane proteins; Polymerase chain reaction (PCR); Thyroid