title: [The effect of heat stress on the cytoskeleton and cell cycle of human umbilical vein endothelial cell in vitro].
abstract: OBJECTIVE
  To study the effect of heat stress on the cytoskeleton and cell cycle of human umbilical vein endothelial cell ( HUVEC ) in vitro.
  
  
  METHODS
  HUVEC was cultured in vitro in 5%CO(2) medium at 37 centigrade ( control group ) or 43 centigrade ( heat stress group ) for 1 hour. Coomassie brilliant blue R-250 staining was used to determine the effect of heat stress on the cytoskeleton. The cells in heat stress group were subsequently cultured at 37 centigradein 5%CO(2) medium after heat stress for 1 hour, and cell cycle of HUVEC was determined at 0, 6, 12, 18 and 24 hours with flow cytometry.
  
  
  RESULTS
  Under light microscopy normal cytoskeleton was observed in control group, but thicker and shorter cytoskeleton was found after a rise of temperature, and stress fibers were found in heat stress group. The DNA content of HUVEC at all time points in G0/G1 stage was 38.07%-55.19% after heat stress. The DNA content in control group was 48.57%, and it was 54.06%, 55.19%, 48.23%, 38.07%, and 41.03% at 0, 6, 12, 18, 24 hours in G0/G1 stage in heat stress group. DNA content in S phase was 35.33%-48.18%. The DNA content in control group was 44.62%, and it was 35.33%, 39.50%, 42.50%, 48.18%, and 47.99% at 0, 6, 12, 18, 24 hours in S stage in heat stress group. DNA content in G2/M phase was 5.31%-13.75%. The DNA content in control group was 6.81, and it was 10.61%, 5.31%, 9.27%,13.75%, and 10.98% at 0, 6, 12, 18, 24 hours in G2/M stage in heat stress group. It was demonstrated that compared with control group, the DNA content in G0/G1 stage was significantly increased when the HUVEC were separated from heat stress within 6 hours, and it recovered at a similar level as control group at 12 hours.
  
  
  CONCLUSIONS
  Heat stress can change the cytoskeleton of HUVEC, and cause stagnation at G0/G1 stage in cell cycle.
authors.affiliation: Department of Critical Care Medicine, Guangzhou General Hospital of Guangzhou Military Command, the Military Key Laboratory of Trauma Care in Hot Zone and Tissue Repair in PLA, Guangzhou 510010, Guangdong, China. Corresponding author: Su Lei, Email: slei_icu@163.com.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Pan
authors.forename: Yu
authors.initials: Y
authors.lastname: Shao
authors.forename: Yan
authors.initials: Y
authors.lastname: Geng
authors.forename: Jinghe
authors.initials: J
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Su
mesh_terms: D002453:Cell Cycle; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D005434:Flow Cytometry; D018869:Heat-Shock Response; D006358:Hot Temperature / Q000009:adverse effects; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques