title: Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.
abstract: Although chromosome partitioning during mitosis is well studied, the molecular mechanisms that allow proper segregation of cytoplasmic organelles in human cells are poorly understood. Here we show that mitochondria interact with growing microtubule tips and are transported towards the daughter cell periphery at the end of mitosis. This phenomenon is promoted by the direct and cell cycle-dependent interaction of the mitochondrial protein Miro and the cytoskeletal-associated protein Cenp-F. Cenp-F is recruited to mitochondria by Miro at the time of cytokinesis and associates with microtubule growing tips. Cells devoid of Cenp-F or Miro show decreased spreading of the mitochondrial network as well as cytokinesis-specific defects in mitochondrial transport towards the cell periphery. Thus, Miro and Cenp-F promote anterograde mitochondrial movement and proper mitochondrial distribution in daughter cells.
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Gil
authors.initials: G
authors.lastname: Kanfer
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Thibault
authors.initials: T
authors.lastname: Courthéoux
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Peterka
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Sonja
authors.initials: S
authors.lastname: Meier
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Soste
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Andre
authors.initials: A
authors.lastname: Melnik
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
authors.forename: Katarina
authors.initials: K
authors.lastname: Reis
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
authors.forename: Pontus
authors.initials: P
authors.lastname: Aspenström
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Peter
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Paola
authors.initials: P
authors.lastname: Picotti
authors.affiliation: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Benoît
authors.initials: B
authors.lastname: Kornmann
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D008840:Microfilament Proteins; D024101:Mitochondrial Proteins; C094772:centromere protein F; C473124:RHOT1 protein, human; C473125:RHOT2 protein, human; D020741:rho GTP-Binding Proteins