title: Lipopolysaccharide Attenuates CD40 Ligand-Induced Regulatory B10 Cell Expansion and IL-10 Production in Mouse Splenocytes.
abstract: Toll-like receptors (TLRs) play a key role in B cell-mediated innate and adaptive immunity. It has been shown that interleukin 10 (IL-10)-producing regulatory B cells (B10 cells) can negatively regulate cellular immune responses and inflammation in autoimmune diseases. In this study, we determined the effect of TLR4 signaling on the CD40-activated B10 cell competency. The results demonstrated that LPS and CD40L synergistically stimulated proliferation of mouse splenocytes. The percentage of B10 cells in cultured splenocytes was significantly increased after CD40L stimulation but such increase was diminished by the addition of LPS. Such effects by LPS were only observed in cells from WT but not TLR4-/-mice. IL-10 mRNA expression and protein production in B10 cells from cultured splenocytes were significantly up-regulated by CD40L stimulation but were inhibited after the addition of LPS in a TLR4-dependent manner. This study suggests that LPS-induced TLR4 signaling attenuate CD40L-activated regulatory B10 cell competency.
authors.affiliation: Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, USA ; Department of Stomatology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, USA ; Department of Stomatology, Fourth Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, USA ; Department of Stomatology, Shanghai 9th People's Hospital, Shanghai, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, USA.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonheur
authors.affiliation: Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, USA.
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Department of Stomatology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, USA.
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Han
keywords: B10; CD40L; IL10; LPS; TLR4