title: Inhibitory effect of zinc on the advanced glycation end product-induced apoptosis of mouse osteoblastic cells.
abstract: Osteoporosis and diabetes have become serious health problems worldwide. Previous studies have suggested that diabetes is associated with osteoporosis and increased fracture risk. However, the mechanism underlying diabetes‑induced osteoporosis remains to be elucidated. Therefore, the present study aimed to examine the mechanism underlying diabetes‑induced osteoporosis, and determine the protective effects of zinc, which is known to be closely associated with osteoporosis and diabetes. The results of the present study demonstrated that zinc inhibited advanced glycation end product (AGE)‑induced MC3T3‑E1 cell apoptosis by attenuating the production of reactive oxygen species, inhibiting caspase‑3 and caspase‑9 activation, and inhibiting the release of cytochrome c from between the mitochondria and the cytosol. Furthermore, zinc was found to protect cells against AGE‑induced apoptosis via the mitogen‑activated protein kinase/extracellular signal‑regulated kinase and phosphoinositide 3‑kinase/AKT signaling pathways. In conclusion, these findings enable a better understanding of the mechanism underlying diabetes‑induced osteoporosis, and may indicate a novel target for its prevention and treatment.
authors.affiliation: Department of Traumatic Surgery, Xinqu Hospital, The First Affiliated Hospital of Henan University of Science Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Xiong
authors.affiliation: Department of Traumatic Surgery, Xinqu Hospital, The First Affiliated Hospital of Henan University of Science Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Traumatic Surgery, Xinqu Hospital, The First Affiliated Hospital of Henan University of Science Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Traumatic Surgery, Xinqu Hospital, The First Affiliated Hospital of Henan University of Science Technology, Luoyang, Henan 471000, P.R. China.
authors.forename: Hangfei
authors.initials: H
authors.lastname: Gao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*
chemical_list: D017127:Glycation End Products, Advanced; D017382:Reactive Oxygen Species; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D053453:Caspase 9; D015032:Zinc