title: Functional characterization of PCRK1, a putative protein kinase with a role in immunity.
abstract: In Arabidopsis, defense signaling is triggered by the perception of conserved molecular patterns by pattern recognition receptors (PRRs). Signal transduction from the PRRs requires members of a family of Receptor-Like Cytoplasmic Kinases (RLCKs). Previously, we described one such RLCK, PTI Compromised Receptor-Like Cytoplasmic Kinase 1 (PCRK1) that is important for immunity induced by Microbe Associated Molecular Patterns (MAMPs) as well as Damage Associated Molecular Patterns (DAMPs). In this study, we measured the growth of Pma ES4326 in double mutants carrying pcrk1 together with the salicylic acid (SA) biosynthesis mutation sid2-2 or the jasmonic acid (JA) receptor mutation coi1-1, showing that the function of PCRK1 is SA independent but may be partially dependent on JA. Mutation of phosphorylated serine residues S232, S233 and S237 compromised the immune signaling function of PCRK1.
authors.affiliation: a Department of Plant Biology and Microbial and Plant Genomics Institute ; University of Minnesota ; Saint Paul , MN USA.
authors.forename: Suma
authors.initials: S
authors.lastname: Sreekanta
authors.affiliation: b Department of Biochemistry ; University of Wisconsin-Madison ; Madison , WI USA.
authors.forename: Miyoshi
authors.initials: M
authors.lastname: Haruta
authors.affiliation: b Department of Biochemistry ; University of Wisconsin-Madison ; Madison , WI USA.
authors.forename: Benjamin B
authors.initials: BB
authors.lastname: Minkoff
authors.affiliation: a Department of Plant Biology and Microbial and Plant Genomics Institute ; University of Minnesota ; Saint Paul , MN USA.
authors.forename: Jane
authors.initials: J
authors.lastname: Glazebrook
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D057865:Plant Immunity; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D051192:Receptors, Pattern Recognition; C011006:jasmonic acid; C000606408:PCRK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D020156:Salicylic Acid
keywords: Pattern Triggered Immunity (PTI); microbe-associated molecular pattern; receptor-like cytoplasmic kinase