title: Ceratocystis tiliae sp. nov., a wound pathogen on Tilia americana.
abstract: Species in the North American clade (NAC) of the Ceratocystis fimbriata complex are mostly weak pathogens that infect native tree hosts through fresh wounds. Isolations from discolored tissue of wounded Tilia americana (basswood) in Iowa and Nebraska yielded a Ceratocystis species that was similar to but distinct from isolates of C. variospora from other hosts. Sequences of 28S rDNA showed that isolates from basswood did not differ from C. variospora, but there were minor differences in ITS rDNA sequences. The DNA sequences of a portion of the Cerato-platanin gene and TEF1α showed the basswood fungus to be a unique lineage. Cross inoculations in two experiments showed that the basswood isolates and C. variospora isolates from Quercus spp. were most aggressive to their respective hosts. Isolates from basswood grew slower and were less pigmented than C. variospora isolates from Quercus spp. The basswood fungus thus is distinguished from C. variospora based on phylogenetic analyses and phenotype and is herein described as C. tiliae sp. nov.
authors.affiliation: Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: L S S
authors.initials: LS
authors.lastname: Oliveira
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, 351 Bessey Hall, Ames, Iowa 50011 tcharrin@iastate.edu.
authors.forename: T C
authors.initials: TC
authors.lastname: Harrington
authors.affiliation: Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: R G
authors.initials: RG
authors.lastname: Freitas
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, 351 Bessey Hall, Ames, Iowa 50011.
authors.forename: D
authors.initials: D
authors.lastname: McNew
authors.affiliation: Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Alfenas
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D032384:Tilia / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: C. variospora; Ceratocystis fimbriata; ceratocystis wilt; genetic variability