title: Application of dopaminergic antagonist: Metoclopramide, in reproduction of crucian carp Carassius carassius (L.) under controlled conditions.
abstract: The effectiveness of Ovopel (two pellets/kg body weight), a complex of [D-Ala(6) Pro(9)NEt-mGnRH] and dopamine antagonist (DA e.g. Metoclopramide, MET), was compared with the application MET alone at the dose of 20mg/kg body weight in spawning crucian carp Carassius carassius (L.) in the present study. Ovulation and spermation rates were greater after MET application (90% and 100%, respectively) in comparison to the Ovopel (80% and 87.5%, respectively) when used as a broodstock reproductive stimulation agent. Sperm quality (CASA parameters) after MET treatment was very acceptable without a significant decrease in comparison to the samples from Ovopel-treated crucian carp when spermation stimulation treatments were employed. Though there were no differences in egg and sperm production between Ovopel and MET treatments, hatching rates were significantly greater after MET (84.4%) compared with Ovopel (76.2%) treatment. The usefulness of MET application without GnRHa addition for stimulation of reproduction was ascertained in the present study for the first time in crucian carp.
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Olsztyn, Poland. Electronic address: b.cejko@pan.olsztyn.pl.
authors.forename: Beata Irena
authors.initials: BI
authors.lastname: Cejko
authors.affiliation: Department of Lake and River Fisheries, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kucharczyk
mesh_terms: D000818:Animals; D002347:Carps; D018492:Dopamine Antagonists / Q000494:pharmacology*; D004625:Embryo, Nonmammalian; D005260:Female; D005306:Fertilization / Q000187:drug effects; D008297:Male; D008787:Metoclopramide / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects*; D012661:Semen; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D018492:Dopamine Antagonists; D008787:Metoclopramide
keywords: Crucian carp; Eggs; Metoclopramide; Ovopel; Reproduction; Sperm