title: Delftia tsuruhatensis WGR-UOM-BT1, a novel rhizobacterium with PGPR properties from Rauwolfia serpentina (L.) Benth. ex Kurz also suppresses fungal phytopathogens by producing a new antibiotic-AMTM.
abstract: The bacterial strain designated as WGR-UOM-BT1 isolated from rhizosphere of Rauwolfia serpentina exhibited broad-spectrum antifungal activity and also improved early plant growth. Based on morphological, biochemical and 16S rRNA gene sequence analyses, the strain BT1 was identified as Delftia tsuruhatensis (KF727978). Under in vitro conditions, the strain BT1 suppressed the growth of wide range of fungal phytopathogens. Purified antimicrobial metabolite from the strain BT1 was identified as nitrogen-containing heterocyclic compound, 'amino(5-(4-methoxyphenyl)-2-methyl-2-(thiophen-2-yl)-2,3-dihydrofuran-3-yl)methanol' (AMTM), with molecular mass of 340•40 and molecular formula of C17 H19 NO3 S. The strain BT1 was positive for rhizosphere colonization (tomato), IAA production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and phosphate solubilization. Under laboratory and greenhouse conditions, the strain BT1 promoted plant growth and suppressed foliar and root fungal pathogens of tomato. Therefore, antimicrobial and disease protection properties of strain BT1 could serve as an effective biological control candidate against devastating fungal pathogens of vegetable plants. Besides, the production of IAA, P solubilization and ACC deaminase activity enhance its potential as a biofertilizer and may stabilize the plant performance under fluctuating environmental conditions.
  
  
  CONCLUSIONS
  In this study, we reported that Delftia tsuruhatensis WGR-UOM-BT1 strain has the plant growth promotion activities such as rhizosphere colonization (tomato), IAA production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and phosphate solubilization. This bacterial strain was found producing an antimicrobial nitrogen-containing heterocyclic compound identified as 'amino(5-(4-methoxyphenyl)-2-methyl-2-(thiophen-2-yl)-2,3-dihydrofuran-3-yl)methanol' [C17 H19 NO3 S] (AMTM), which is new to the bacterial world.
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
authors.forename: S P
authors.initials: SP
authors.lastname: Prasannakumar
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
authors.forename: H G
authors.initials: HG
authors.lastname: Gowtham
authors.affiliation: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
authors.forename: P
authors.initials: P
authors.lastname: Hariprasad
authors.affiliation: Manipal Centre for Natural Sciences, Manipal University, Manipal, Udupi, Karnataka, India.
authors.forename: K
authors.initials: K
authors.lastname: Shivaprasad
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Niranjana
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000378:metabolism*; D061046:Biological Control Agents / Q000494:pharmacology*; D019755:Carbon-Carbon Lyases / Q000737:chemistry; D042742:Delftia / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism; D018551:Solanum lycopersicum / Q000382:microbiology; D063245:Plant Development; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011926:Rauwolfia / Q000382:microbiology*; D012231:Rhizobium / Q000235:genetics; D058441:Rhizosphere; D013876:Thiophenes / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents; D007210:Indoleacetic Acids; D012336:RNA, Ribosomal, 16S; D013876:Thiophenes; C000607600:amino(5-(4-methoxyphenyl)-2-methyl-2-(thiophen-2-yl)-2,3-dihydrofuran-3-yl)methanol; C030737:indoleacetic acid; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: AMTM; Alternaria solani; Delftia tsuruhatensis; Fusarium oxysporum; antagonism