title: Studies on the synthesis, characterization, human serum albumin binding and biological activity of single chain surfactant-cobalt(III) complexes.
abstract: The interaction of surfactant-cobalt(III) complexes [Co(bpy)(dien)TA](ClO4)3 · 3H2O (1) and [Co(dien)(phen)TA](ClO4)3 · 4H2O (2), where bpy = 2,2'-bipyridine, dien = diethylenetriamine, phen = 1,10-phenanthroline and TA = tetradecylamine with human serum albumin (HSA) under physiological conditions was analyzed using steady state, synchronous, 3D fluorescence, UV/visabsorption and circular dichroism spectroscopic techniques. The results show that these complexes cause the fluorescence quenching of HSA through a static mechanism. The binding constant (Kb ) and number of binding-sites (n) were obtained at different temperatures. The corresponding thermodynamic parameters (∆G°, ∆H° and ∆S°) and Ea were also obtained. According to Förster's non-radiation energy transfer theory, the binding distance (r) between the complexes and HSA were calculated. The results of synchronous and 3D fluorescence spectroscopy indicate that the binding process has changed considerably the polarity around the fluorophores, along with changes in the conformation of the protein. The antimicrobial and anticancer activities of the complexes were tested and the results show that the complexes have good activities against pathogenic microorganisms and cancer cells.
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Vignesh
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Sugumar
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Arunachalam
authors.affiliation: Department of Marine Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Vignesh
authors.affiliation: Department of Marine Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Arthur James
authors.affiliation: Department of Biomedical Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Arun
authors.affiliation: Department of Biomedical Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Premkumar
mesh_terms: D001665:Binding Sites; D016923:Cell Death / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019169:Jurkat Cells; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry* / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009942:Organometallic Compounds; D012709:Serum Albumin; D013501:Surface-Active Agents; D003035:Cobalt
keywords: activation energy; circular dichroism; human serum albumin; inner filter effect; surfactant-cobalt(III) complexes