title: Anti-Glycation Effects of Pomegranate (Punica granatum L.) Fruit Extract and Its Components in Vivo and in Vitro.
abstract: Accumulation of advanced glycation end products (AGEs) leads to various diseases such as diabetic complications and arteriosclerosis. In this study, we examined the effect of pomegranate fruit extract (PFE) and its constituent polyphenols on AGE formation in vivo and in vitro. PFE, fed with a high-fat and high-sucrose (HFS) diet to KK-A(y) mice, significantly reduced glycation products such as glycoalbumin (22.0 ± 2.4%), hemoglobin A1c (5.84 ± 0.23%), and serum AGEs (8.22 ± 0.17 μg/mL), as compared to a control HFS group (30.6 ± 2.6%, 7.45 ± 0.12%, and 9.55 ± 0.17 μg/mL, respectively, P < 0.05). In antiglycation assays, PFE, punicalin, punicalagin, ellagic acid, and gallic acid suppressed the formation of AGEs from bovine serum albumin and sugars. In this study, we discuss the mechanism of the antiglycation effects of PFE and its components in vivo and in vitro.
authors.affiliation: Department of Pharmaceutial and Health Sciences, Faculty of Pharmaceutical Sciences, Josai University , Saitama, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kumagai
authors.affiliation: Department of Pharmaceutial and Health Sciences, Faculty of Pharmaceutical Sciences, Josai University , Saitama, Japan.
authors.forename: Sachie
authors.initials: S
authors.lastname: Nakatani
authors.affiliation: Department of Pharmaceutial and Health Sciences, Faculty of Pharmaceutical Sciences, Josai University , Saitama, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Onodera
authors.affiliation: Morishita Jintan Co., Ltd. , Osaka, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Nagatomo
authors.affiliation: Morishita Jintan Co., Ltd. , Osaka, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Nishida
authors.affiliation: Morishita Jintan Co., Ltd. , Osaka, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Matsuura
authors.affiliation: Department of Pharmaceutial and Health Sciences, Faculty of Pharmaceutical Sciences, Josai University , Saitama, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kobata
authors.affiliation: Department of Pharmaceutial and Health Sciences, Faculty of Pharmaceutical Sciences, Josai University , Saitama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Wada
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects*; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D059808:Polyphenols / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D059808:Polyphenols
keywords: advanced glycation end products (AGEs); diabetes; ellagic acid; pomegranate; punicalagin