title: Effect of jiaweiwumel decoction on regulatory T cells and interleukin-10 in a rat model of ulcerative colitis.
abstract: OBJECTIVE
  To investigate the effect of a decoction made from the Traditional Chinese Medicine wumei pill, on regulatory T cells and interleukin-10 (IL-10) in a rat model of ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS).
  
  
  METHODS
  Rat ulcerative colitis was induced with TNBS. All modeled rats were randomly divided into six groups: normal control group; model group; sulfasalazine suppositories treatment group; and high, moderate, and low dosage of jiaweiwumei decoction groups (12 rats each). Colon injury index was evaluated after 14 days. After peripheral blood lymphocyte separation, CD4+ T cells and CD4+/CD25+ T cell percentage was detected by flow cytometry. The content of IL-10 in serum and intestinal mucosa tissue was detected by sandwich enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Colon injury indices in the decoction groups were effectively reduced, compared with the model group (P < 0.05). Compared with that of the control group, the CD4+/CD25+ to CD4+ T lymphocyte ratio of the model group was significantly lower, while the decoction treatment improved the CD4 +/CD25 + to CD4 + T lymphocyte ratio (P < 0.05). The serum and mucosal IL-10 content of the model group was significantly lower (P < 0.05) than that in the control group, while the decoction group had significantly higher serum and intestinal mucosal IL-10 content than that in the model group (P < 0.05). The regulatory T cell content was negatively correlated with the colonic injury index (r = 0.68, P < 0.05), and positively correlated with the content of serum IL-10 (r= 0.87, P < 0.05) and intestinal mucosal IL-10 (r= 0.79, P < 0.05).
  
  
  CONCLUSIONS
  Jiaweiwumei decoction had significant effects on regulatory T cells and IL-10 in rats with TNBS-induced ulcerative colitis.
authors.forename: Mei
authors.initials: M
authors.lastname: Tao
authors.forename: Xuyan
authors.initials: X
authors.lastname: Wang
authors.forename: Aiqin
authors.initials: A
authors.lastname: Wang
authors.forename: Ninghan
authors.initials: N
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
chemical_list: D004365:Drugs, Chinese Herbal; D016753:Interleukin-10