title: [Effects of polydatin on learning and memory and Cdk5 kinase activity in the hippocampus of rats with chronic alcoholism].
abstract: OBJECTIVE
  To observe the effects of polydatin on learning and memory and cyclin-dependent kinase 5 (Cdk5) kinase activity in the hippocampus of rats with chronic alcoholism.
  
  
  METHODS
  Forty rats were randomly divided into 4 groups: control group, chronic alcoholism group, low and high polydatin group. The rat chronic alcoholism model was established by ethanol 3.0 g/(kg · d) (intragastric administration). The abstinence scoring was used to evaluate the rats withdrawal symptoms; cognitive function was measured by Morris water maze experiment; Cdk5 protein expression in the hippocampus was detected by immunofluorescence; Cdk5 kinase activity in the hippocampus was detected by liquid scintillation counting method.
  
  
  RESULTS
  The abstinence score, escape latency, Cdk5 kinase activity in chronic alcoholism group rats were significantly higher than those of control group (P < 0.05). The abstinence score, escape latency in high polydatin group rats were significantly lower than those of chronic alcoholism group (P < 0.05); Cdk5 kinase activity in high and low polydatin group rats was significantly lower than that of chronic alcoholism group( P < 0.05); immunofluorescence showed that the Cdk5 positive cells of chronic alcoholism group were significantly increased compared with control group (P < 0.05), and the Cdk5 positive cells of polydatin groups were significantly decreased compared with chronic alcoholism group ( P < 0.05).
  
  
  CONCLUSIONS
  Polydatin-reduced the chronic alcoholism damage may interrelate with regulation of Cdk5 kinase activity.
authors.forename: Xin-juan
authors.initials: XJ
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Ai-lin
authors.initials: AL
authors.lastname: Du
authors.forename: Li-bin
authors.initials: LB
authors.lastname: Zhang
authors.forename: Rui-ling
authors.initials: RL
authors.lastname: Zhang
mesh_terms: D000437:Alcoholism / Q000503:physiopathology*; D000818:Animals; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D007858:Learning / Q000187:drug effects*; D008568:Memory / Q000187:drug effects*; D051381:Rats; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes; D051360:Cyclin-Dependent Kinase 5; C495907:Cdk5 protein, rat; C058229:polydatin