title: Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence.
abstract: Puccinia striiformis f. sp. tritici (Pst) is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatellite markers from the genomic sequence of an isolate of Chinese Pst race CY32. Polymorphism at each simple sequence repeat (SSR) locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 (mean 0.62) and 0.23 to 0.73 (mean 0.51), respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag (EST)-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
authors.forename: Gang-ming
authors.initials: GM
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
authors.forename: Fu-ping
authors.initials: FP
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
authors.forename: Huai-yong
authors.initials: HY
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
authors.forename: Shu-chang
authors.initials: SC
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Wen-ming
authors.initials: WM
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China; kangzs@nwsuaf.edu.cn.
authors.forename: Zhen-sheng
authors.initials: ZS
authors.lastname: Kang
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D016681:Genome, Fungal / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D005819:Genetic Markers
keywords: Microsatellites; Simple sequence repeat; Stripe rust