title: Ovine Hair Follicle Stem Cells Derived from Single Vibrissae Reconstitute Haired Skin.
abstract: Hair follicle stem cells (HFSCs) possess fascinating self-renewal capacity and multipotency, which play important roles in mammalian hair growth and skin wound repair. Although HFSCs from other mammalian species have been obtained, the characteristics of ovine HFSCs, as well as the methods to isolate them have not been well addressed. Here, we report an efficient strategy to obtain multipotent ovine HFSCs. Through microdissection and organ culture, we obtained keratinocytes that grew from the bulge area of vibrissa hair follicles, and even abundant keratinocytes were harvested from a single hair follicle. These bulge-derived keratinocytes are highly positive for Krt15, Krt14, Tp63, Krt19 and Itga6; in addition to their strong proliferation abilities in vitro, these keratinocytes formed new epidermis, hair follicles and sebaceous glands in skin reconstitution experiments, showing that these are HFSCs from the bulge outer root sheath. Taken together, we developed an efficient in vitro system to enrich ovine HFSCs, providing enough HFSCs for the investigations about the ovine hair cycle, aiming to promote wool production in the future.
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. zhanghs@ioz.ac.cn.
authors.forename: Huishan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Histology & Embryology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. sdrzzhang@163.com.
authors.forename: Shoubing
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. zhaohuashan@gmail.com.|University of Chinese Academy of Sciences, Beijing 100049, China. zhaohuashan@gmail.com.
authors.forename: Huashan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. qiaojingqiao732@163.com.
authors.forename: Jingqiao
authors.initials: J
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. liushuang@ioz.ac.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. dengzhili@ioz.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. dengzhili@ioz.ac.cn.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. leixh@ioz.ac.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. ningln@ioz.ac.cn.
authors.forename: Lina
authors.initials: L
authors.lastname: Ning
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. caoyj@ioz.ac.cn.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. zhaoy@ioz.ac.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. duane@ioz.ac.cn.
authors.forename: Enkui
authors.initials: E
authors.lastname: Duan
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D039503:Integrin alpha6 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D012756:Sheep; D014738:Vibrissae / Q000166:cytology
chemical_list: D039503:Integrin alpha6; D007633:Keratins
keywords: hair follicle stem cells; keratinocytes; microdissection; organ culture; sheep; skin reconstitution