title: Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts.
abstract: Dedifferentiation and proliferation of endogenous cardiomyocytes in situ can effectively improve cardiac repair following myocardial infarction (MI). 6-Bromoindirubin-3-oxime (BIO) and insulin-like growth factor 1 (IGF-1) are two potent factors that promote cardiomyocyte survival and proliferation. However, their delivery for sustained release in MI-affected areas has proved to be challenging. In the current research, we present a study on the sustained co-delivery of BIO and IGF-1 in a hybrid hydrogel system to simulate endogenous cardiac repair in an MI rat model. Both BIO and IGF-1 were efficiently encapsulated in gelatin nanoparticles, which were later cross-linked with the oxidized alginate to form a novel hybrid hydrogel system. The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function. Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.
authors.affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, People's Republic of China ; Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, People's Republic of China.
authors.forename: Shupei
authors.initials: S
authors.lastname: Qiao
authors.affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, SK, Canada ; Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Xiongbiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Biomedical and Life Sciences, Cardiff University, Cardiff, UK.
authors.forename: Bing
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Hou
authors.affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, People's Republic of China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Tian
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D007211:Indoles / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D009203:Myocardial Infarction / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010091:Oximes / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D012038:Regeneration
chemical_list: C483321:6-bromoindirubin-3'-oxime; D004337:Drug Carriers; D007211:Indoles; D010091:Oximes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007334:Insulin-Like Growth Factor I
keywords: cardiomyocyte proliferation; gelatin nanoparticle; injectable hydrogel; myocardial infarction