title: [Expression of Zonula Occludens-1 in Cerebral Cortex Following Traumatic Brain Injury].
abstract: OBJECTIVE
  To observe the time-course expression of zonula occludens-1 (ZO-1) in cerebral cortex after traumatic brain injury (TBI).
  
  
  METHODS
  The TBI model of mouse was established. The mice were divided in 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, 7 d after TBI, sham and control groups. The permeability of the blood brain barrier was evaluated by measuring the extravasation of Evans blue (EB) dye. The expression of ZO-1 in cerebral cortex in the injured area was detected by Western blotting and immunohistochemistry.
  
  
  RESULTS
  The extravasation of EB dye of injured cortex gradually increased from 1 h, peaked at 1-3 d and approximately decreased to normal at 7 d after TBI. Western blotting revealed that the expression of ZO-1 gradually decreased after 1 h, was at the lowest at 1-3 d, and then significantly increased after 7 d but was still lower than that of normal and sham groups. The result of immunohistochemistry showed that ZO-1 had strong expression in vessel of normal cortex, gradually decreased after TBI, and almost disappeared at 3 d after TBI and gradually recovered to normal level later.
  
  
  CONCLUSIONS
  The expression of ZO-1 in the injured cortex after TBI initially decreases and then increases. The negative correlation between ZO-1 expression and EB extravasation after TBI could be used as a new indicator for wound age estimation.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.forename: Dong-hua
authors.initials: DH
authors.lastname: Zou
authors.forename: Zheng-dong
authors.initials: ZD
authors.lastname: Li
authors.forename: Yi-jiu
authors.initials: YJ
authors.lastname: Chen
authors.forename: Lu-yang
authors.initials: LY
authors.lastname: Tao
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D015153:Blotting, Western; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D010539:Permeability; D019108:Tight Junctions / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*
chemical_list: D062826:Zonula Occludens-1 Protein