title: Role of complement receptor 1 (CR1; CD35) on epithelial cells: A model for understanding complement-mediated damage in the kidney.
abstract: The regulators of complement activation gene cluster encodes a group of proteins that have evolved to control the amplification of complement at the critical step of C3 activation. Complement receptor 1 (CR1) is the most versatile of these inhibitors with both receptor and regulatory functions. While expressed on most peripheral blood cells, the only epithelial site of expression in the kidney is by the podocyte. Its expression by this cell population has aroused considerable speculation as to its biologic function in view of many complement-mediated renal diseases. The goal of this investigation was to assess the role of CR1 on epithelial cells. To this end, we utilized a Chinese hamster ovary cell model system. Among our findings, CR1 reduced C3b deposition by ∼ 80% during classical pathway activation; however, it was an even more potent regulator (>95% reduction in C3b deposition) of the alternative pathway. This inhibition was primarily mediated by decay accelerating activity. The deposited C4b and C3b were progressively cleaved with a t½ of ∼ 30 min to C4d and C3d, respectively, by CR1-dependent cofactor activity. CR1 functioned intrinsically (i.e, worked only on the cell on which it was expressed). Moreover, CR1 efficiently and stably bound but didn't internalize C4b/C3b opsonized immune complexes. Our studies underscore the potential importance of CR1 on an epithelial cell population as both an intrinsic complement regulator and an immune adherence receptor. These results provide a framework for understanding how loss of CR1 expression on podocytes may contribute to complement-mediated damage in the kidney.
authors.affiliation: Washington University School of Medicine, Department of Internal Medicine, Division of Nephrology, 660 South Euclid Avenue, St. Louis, MO 63110 USA. Electronic address: ajava@dom.wustl.edu.
authors.forename: Anuja
authors.initials: A
authors.lastname: Java
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, 660 South Euclid Avenue, St. Louis, MO 63110 USA. Electronic address: kliszews1@dom.wustl.edu.
authors.forename: M Kathryn
authors.initials: MK
authors.lastname: Liszewski
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, 660 South Euclid Avenue, St. Louis, MO 63110 USA. Electronic address: dhourcad@dom.wustl.edu.
authors.forename: Dennis E
authors.initials: DE
authors.lastname: Hourcade
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, 660 South Euclid Avenue, St. Louis, MO 63110 USA. Electronic address: fzhang@shrinenet.org.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, 660 South Euclid Avenue, St. Louis, MO 63110 USA. Electronic address: jatkinso@dom.wustl.edu.
authors.forename: John P
authors.initials: JP
authors.lastname: Atkinson
mesh_terms: D000818:Animals; D016466:CHO Cells; D015935:Complement C4b; D003170:Complement Pathway, Alternative / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D006224:Cricetinae; D003412:Cricetulus; D004847:Epithelial Cells / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology*; D007700:Kinetics; D018448:Models, Immunological; D009895:Opsonin Proteins / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection
chemical_list: C509991:CR1 protein, human; D009895:Opsonin Proteins; D017463:Receptors, Complement 3b; D015935:Complement C4b; D003165:Complement System Proteins
keywords: Cofactor activity; Complement activation; Complement receptor 1; Decay accelerating activity; Epithelial cells; Immune complexes