title: SERS analysis of serum for detection of early and locally advanced breast cancer.
abstract: In this contribution, we investigated whether surface-enhanced Raman scattering (SERS) of serum can be a candidate method for detecting "luminal A" breast cancer (BC) at different stages. We selected three groups of participants aged over 50 years: 20 healthy women, 20 women with early localized small BC, and 20 women affected by BC with lymph node involvement. SERS revealed clear spectral differences between these three groups. A predictive model using principal component analysis (PCA) and linear discriminant analysis (LDA) was developed based on spectral data, and its performance was estimated with cross-validation. PCA-LDA of SERS spectra could distinguish healthy from BC subjects (sensitivity, 92 %; specificity, 85 %), as well as subjects with BC at different stages, with a promising diagnostic performance (sensitivity and specificity, ≥80 %; overall accuracy, 84 %). Our data suggest that SERS spectroscopy of serum, combined with multivariate data analysis, represents a minimally invasive, easy to use, and fast approach to discriminate healthy from BC subjects and even to distinguish BC at different clinical stages.
authors.affiliation: CRO-Biobank, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.|Clinical Cancer Pathology, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cervo
authors.affiliation: Division of Experimental Oncology 2, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.|Dept. of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127, Trieste, TS, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Mansutti
authors.affiliation: Dept. of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127, Trieste, TS, Italy.
authors.forename: Greta
authors.initials: G
authors.lastname: Del Mistro
authors.affiliation: Division of Experimental Oncology 2, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Spizzo
authors.affiliation: Division of Experimental Oncology 2, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.|Centre of Excellence MAT, Dept. of Medical and Biological Science, University of Udine, P.le M. Kolbe 4, 33010, Udine, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Colombatti
authors.affiliation: CRO-Biobank, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.|Clinical Cancer Pathology, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy.
authors.forename: Agostino
authors.initials: A
authors.lastname: Steffan
authors.affiliation: Dept. of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127, Trieste, TS, Italy.
authors.forename: Valter
authors.initials: V
authors.lastname: Sergo
authors.affiliation: Dept. of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127, Trieste, TS, Italy. abonifacio@units.it.
authors.forename: Alois
authors.initials: A
authors.lastname: Bonifacio
mesh_terms: D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D006801:Humans; D008875:Middle Aged
keywords: Breast cancer; Diagnosis; Nanoparticles; Raman; SERS; Serum