title: Evaluation of Physarum polycephalum plasmodial growth and lipid production using rice bran as a carbon source.
abstract: BACKGROUND
  The myxomycete Physarum polycephalum appears to have remarkable potential as a lipid source for biodiesel production. The present study evaluated the use of rice bran as a carbon source and determined the medium components for optimum growth and lipid production for this organism.
  
  
  RESULTS
  Optimization of medium components by response surface methodology showed that rice bran and yeast extract had significant influences on lipid and biomass production. The optimum medium consisted of 37.5 g/L rice bran, 0.79 g/L yeast extract and 12.5 g/L agar, and this yielded 7.5 g/L dry biomass and 0.9 g/L lipid after 5 days. The biomass and lipid production profiles revealed that these parameters increased over time and reached their maximum values (10.5 and 1.26 g/L, respectively) after 7 days. Physarum polycephalum growth decreased on the spent medium but using the latter increased total biomass and lipid concentrations to 14.3 and 1.72 g/L, respectively.
  
  
  CONCLUSIONS
  An effective method for inoculum preparation was developed for biomass and lipid production by P. polycephalum on a low-cost medium using rice bran as the main carbon source. These results also demonstrated the feasibility of scaling up and reusing the medium for additional biomass and lipid production.
authors.affiliation: School of Biotechnology, Ho Chi Minh International University, Ho Chi Minh City, 70000, Vietnam. ttmhanh@hcmiu.edu.vn.
authors.forename: Hanh
authors.initials: H
authors.lastname: Tran
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas, 72701, USA. slsteph@uark.edu.
authors.forename: Steven
authors.initials: S
authors.lastname: Stephenson
authors.affiliation: Stable Isotope Laboratory, University of Arkansas, Fayetteville, Arkansas, 72701, USA. epolloc@uark.edu.
authors.forename: Erik
authors.initials: E
authors.lastname: Pollock
mesh_terms: D056804:Biofuels; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D005285:Fermentation; D008055:Lipids / Q000096:biosynthesis*; D012275:Oryza / Q000254:growth & development / Q000378:metabolism*; D016839:Physarum polycephalum / Q000254:growth & development / Q000378:metabolism*
chemical_list: D056804:Biofuels; D004043:Dietary Fiber; D008055:Lipids; D002244:Carbon