title: miRNA-regulated gene expression differs in celiac disease patients according to the age of presentation.
abstract: Celiac disease is an intestinal disease which shows different symptoms and clinical manifestations among pediatric and adult patients. These variations could be imputable to age-related changes in gut architecture and intestinal immune system, which could be characterized by gene expression differences possibly regulated by miRNAs. We analyzed a panel of miRNAs and their target genes in duodenal biopsies of Marsh 3AB and 3C pediatric celiac patients, compared to controls. Moreover, to assess variation of expression in plasma samples, we evaluated circulating miRNA levels in controls and patients at diagnosis or on gluten-free diet. We detected a decreased miR-192-5p expression in celiac patients, but no variations in NOD2 and CXCL2, targets previously identified in adults. Conversely, we detected a significant increase in mRNA and protein levels of another target, MAD2L1, protein related to cell cycle control. miR-31-5p and miR-338-3p were down-regulated and their respective targets, FOXP3 and RUNX1, involved in Treg function, resulted up-regulated in celiac patients. Finally, we detected, in celiac patients, an increased expression of miR-21-5p, possibly caused by a regulatory loop with its putative target STAT3, which showed an increased activation in Marsh 3C patients. The analysis of plasma revealed a trend similar to that observed in biopsies, but in presence of gluten-free diet we could not detect circulating miRNAs values comparable to controls. miRNAs and their gene targets showed an altered expression in duodenal mucosa and plasma of celiac disease pediatric patients, and these alterations could be different from adult ones.
authors.affiliation: Department of Health Sciences, School of Medicine, University of Milano-Bicocca, Via Cadore, 48, 20900, Monza, MB, Italy.
authors.forename: Gaia
authors.initials: G
authors.lastname: Buoli Comani
authors.forename: Roberto
authors.initials: R
authors.lastname: Panceri
authors.forename: Marco
authors.initials: M
authors.lastname: Dinelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Biondi
authors.forename: Clara
authors.initials: C
authors.lastname: Mancuso
authors.forename: Raffaella
authors.initials: R
authors.lastname: Meneveri
authors.forename: Donatella
authors.initials: D
authors.lastname: Barisani