title: Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer.
abstract: OBJECTIVE
  The detection of tumor-associated microRNA expression in bronchial lavage (BL) samples of lung cancer patients could improve the non-invasive tumor diagnostic.
  
  
  METHODS
  The profile of extracellular microRNAs in bronchial lavage was evaluated using three pools for lung cancer group (malignant) and three pools for non-cancerous group (benign) of 10 patients each. To confirm the results for the selected microRNAs in a qRT-PCR the BL of 30 different lung cancer and non-cancerous patients was used. We examined total-RNA from cell-free supernatant of BL samples. For normalization we added exogenous cel-miR 39.
  
  
  RESULTS
  Using microRNA arrays we found a panel of eight microRNAs (hsa-miR 19b-1, 1285, 1289, 1303, 217, 29a-5p, 548-3p, 650) that were differentially expressed between the lung cancer and the non-cancerous group. Further investigation by qPCR revealed five microRNAs (U6 snRNA, hsa-miR 1285, 1303, 29a-5p, 650) that were significantly up-regulated in patients with lung cancer.
  
  
  CONCLUSIONS
  In bronchial lavage samples the five microRNAs identified in this study have a diagnostic potential for use as noninvasive biomarkers in lung cancer.
authors.affiliation: Abteilung Pneumologie, Klinik für Innere Medizin 1, Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Straße 40, 06120 Halle/Saale, Germany. Electronic address: grit.rehbein@uk-halle.de.
authors.forename: Grit
authors.initials: G
authors.lastname: Rehbein
authors.affiliation: Abteilung Pneumologie, Klinik für Innere Medizin 1, Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Straße 40, 06120 Halle/Saale, Germany. Electronic address: bernd.schmidt@uk-halle.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Schmidt
authors.affiliation: Abteilung Pneumologie, Klinik für Innere Medizin 1, Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Straße 40, 06120 Halle/Saale, Germany. Electronic address: michael.fleischhacker@uk-halle.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Fleischhacker
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D018893:Bronchoalveolar Lavage; D005110:Extracellular Space / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Bronchial lavage samples; Extracellular microRNA; Lung cancer; qRT-PCR