title: Haemato-biochemical and oxidative status of buffaloes naturally infected with Trypanosoma evansi.
abstract: Blood samples were collected from 05 clinically healthy and 10 adult female water buffaloes naturally infected with Trypanosoma evansi. Confirmation of disease free and infected status of buffaloes was made on clinical signs, observation of T. evansi parasites in the blood smear and duplex PCR based assay. Blood samples were evaluated for levels of haemoglobin (Hb), packed cell volume (PCV), differential leucocytes count (DLC), lipid peroxidation (LPO), calcium, phosphorous, magnesium sodium and potassium and activities of superoxide dismutase (SOD), catalase (CAT), aspartate transaminase (AST), lactate dehydogenase (LDH) and alkaline phosphatase (ALP). The results of the study revealed substantial decrease in levels of Hb, PCV and increase in LPO, SOD, CAT and AST in infected animals compared to healthy animals. However other haematological and biochemical indices did not show significant variations in infected and healthy buffaloes. The enhanced erythrocytic oxidation and reduction of hematological indices, suggests that the enhanced oxidation of the erythrocytes may be a contributory factor in erythrocytic destruction and progression of the anaemia in T. evansi infection in water buffaloes.
authors.affiliation: Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Mathura 281001, India. Electronic address: drvijaypandey@gmail.com.
authors.forename: Vijay
authors.initials: V
authors.lastname: Pandey
authors.affiliation: Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Mathura 281001, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Nigam
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Mathura 281001, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Jaiswal
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Mathura 281001, India.
authors.forename: Vikrant
authors.initials: V
authors.lastname: Sudan
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Mathura 281001, India.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Department of Veterinary Obstetrics and Gynecology, College of Veterinary Science and Animal Husbandry, Mathura 281001, India.
authors.forename: Pramod Kumar
authors.initials: PK
authors.lastname: Yadav
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism; D002020:Buffaloes / Q000097:blood*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015227:Lipid Peroxidation / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014345:Trypanosoma / Q000145:classification*; D014352:Trypanosomiasis / Q000097:blood / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases
keywords: Buffalo; Haemato-biochemical indices; Oxidative status; Trypanosoma evansi