title: [Association of single nucleotide polymorphisms of cytochrome P450 gene with susceptibility to gout in ethnic Han males from coastal regions of Shandong province].
abstract: OBJECTIVE
  To assess the association of cytochrome P450 gene single nucleotide polymorphisms (SNPs) with susceptibility to gout in ethnic Han males from coastal regions of Shandong province.
  
  
  METHODS
  Four hundred and eighty male patients with gout and 480 healthy male controls were included. Genotyping was carried out with a custom Illumina GoldenGate Genotyping assay to detect SNP rs2275620 of CYP2C8 gene, SNP rs2070676 of CYP2E1 gene, SNP rs837395 of CYP4B1 gene, and SNP rs194150 of TBXAS1 gene. The association was assessed with chi-square test.
  
  
  RESULTS
  No significant difference has been found between the two groups in regard to the genotypic and allelic frequencies of the TT, AT, AA genotypes and A, T alleles of the SNP rs2275620 of the CYP2C8 gene (P=0.88; P=0.97), the CC, CG, GG genotypes and C,G alleles of SNP rs2070676 of the CYP2E1 gene (P=0.24; P=0.09), the TT, AT, AA genotypes and A, T alleles of SNP rs837395 of the CYP4B1 (P=0.88; P=0.97), and TT, AT, AA genotypes and the A,T alleles of SNP rs194150 of TBXAS1 gene (P=0.15; P=0.06).
  
  
  CONCLUSIONS
  This study has identified no association of SNP loci rs2275620(A/T) of CYP2C8, rs2070676(C/G) of CYP2E1, rs837395(A/T) of CYP4B1 and rs194150(A/T) of TBXAS1 with gout in ethnic Han males from coastal regions in Shandong province. However, our result needs to be replicated in larger sets of patients collected from other regions and populations.
authors.affiliation: Shandong Provincial Gout Clinical Medical Center, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P. R. China. jiazt@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.forename: Guizhi
authors.initials: G
authors.lastname: Mao
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Changgui
authors.initials: C
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinde
authors.initials: X
authors.lastname: Li
authors.forename: Mingxia
authors.initials: M
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Ren
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Zhaotong
authors.initials: Z
authors.lastname: Jia
mesh_terms: D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D002681:China / Q000208:ethnology; D065727:Cytochrome P-450 CYP2C8 / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D004198:Disease Susceptibility; D005260:Female; D006073:Gout / Q000201:enzymology* / Q000208:ethnology / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013930:Thromboxane-A Synthase / Q000235:genetics*
chemical_list: D019392:Cytochrome P-450 CYP2E1; D001189:Aryl Hydrocarbon Hydroxylases; C450259:CYP2C8 protein, human; D065727:Cytochrome P-450 CYP2C8; C095029:cytochrome P-450 CYP4B1; D013930:Thromboxane-A Synthase