title: Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model.
abstract: The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines (TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcinoma cell as a cell-based model. First, the constructed plasmid (pTα1-Luc) was transfected into P19 cells to establish a screening model. Second, several TCMs were screened using the established model and all-trans-retinoic acid as a positive control. Finally, the underlying molecular mechanism was explored using immunofluorescence staining, qT-PCR, and Western blot analysis. Our results indicated that the drug screen model was established successfully and that both honokiol and hyperoside induced P19 differentiation into neurons, with the possible molecular mechanism being modulating the Wnt signaling pathway. In conclusion, the drug screening model developed in the present study provides a rapid, cell-based screening platform for identifying natural compounds with neuronal differentiation effects.
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: drugscreen@126.com.
authors.forename: Lu-Yong
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: hliao@cpu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liao
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D054278:Embryonal Carcinoma Stem Cells / Q000187:drug effects*; D017705:Lignans / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014212:Tretinoin / Q000502:physiology; D060449:Wnt Signaling Pathway; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011794:Quercetin; D014212:Tretinoin; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C021304:hyperoside
keywords: Drug screening model; Neuronal differentiation; P19 embryonic carcinoma cells; Traditional Chinese Medicine; Wnt signaling pathway