title: Survivin mRNA-circulating tumor cells are associated with prostate cancer metastasis.
abstract: Detection of circulating tumor cells (CTCs) has been made to develop reliable assays for early diagnosis of various cancers. Overexpression of survivin in cancer cells is strongly associated with tumor progression. Although upregulation of survivin is observed in various tumors, its expression profile in the peripheral blood of prostate cancer (PCa) patients has not yet been investigated. In this study, we validated the application of survivin as the tumor marker to detect CTC and assessed its utility for diagnosis of PCa distant metastasis. Immunohistochemistry and quantitative real-time PCR (QRT-PCR) were performed to confirm the levels of surviving expression in PCa tissues. In addition, CTC values in 3 mL of peripheral blood from PCa patients, benign prostate hyperplasia (BPH) patients, and normal controls were also measured by the survivin-targeted PCR. Our results showed that surviving was overexpressed in PCa tissues. The median levels of blood surviving mRNA of PCa patients, BPH patients, and normal controls were 5.67 (range from 0 to 12.46), 2.24 (range from 0 to 6.55), and 1.85 (range from 0 to 3.82), respectively. The levels of survivin are positively associated with PCa distant metastasis. Our results concluded that quantitation of CTCs through survivin-PCR could be a promising marker for diagnosis of PCa metastasis.
authors.affiliation: Department of Urology Surgery, Shandong Cancer Hospital, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China. frofwangsh@163.com.
authors.forename: Huansheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology Surgery, Shandong Cancer Hospital, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China.
authors.forename: Mingshan
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Urology Surgery, Shandong Cancer Hospital, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Urology Surgery, Shandong Cancer Hospital, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China.
authors.forename: Benkui
authors.initials: B
authors.lastname: Zou
authors.affiliation: Department of Urology Surgery, Shandong Cancer Hospital, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Urology Surgery, Shandong Cancer Hospital, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Bian
authors.affiliation: Department of Central Laboratory, Shandong Cancer Hospital, Jinan, Shandong, 250117, People's Republic of China.
authors.forename: Xingwu
authors.initials: X
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin
keywords: Circulating tumor cell; Metastasis; Prostate cancer; Survivin mRNA