title: Antimicrobial peptide lysozyme has the potential to promote mouse hair follicle growth in vitro.
abstract: Lysozyme is a well-known antimicrobial peptide that exists widely in mammalian skin and it is also expressed by pilosebaceous units. However, the exact location of lysozyme in hair follicles and whether it exerts any direct effects on hair follicle growth are unclear. To determine whether lysozyme affected hair growth in vitro, micro-dissected mouse vibrissae follicles (VFs) were treated in serum-free organ culture for 3 days with lysozyme (1-10μg/ml). After that, the effects of lysozyme on dermal papilla (DP) cells were also investigated. Lysozyme was mainly identified in DP and dermal sheath regions of VF by immunochemistry. In addition, 5-10μg/ml lysozyme had a promoting effect on shaft production. It was also associated with significant proliferation of matrix keratinocytes by immunofluorescence observation. Furthermore, lysozyme promoted hair growth by increasing the levels of alkaline phosphatase and lymphoid enhancer factor 1 in DP, as determined by Western blotting. These results indicate that lysozyme is a promoter of VF growth via enhancing the hair-inductive capacity of DP cells during organ culture.
authors.affiliation: Department of Burn and Plastic Surgery, The People's Hospital of Baoan Shenzhen Affiliated to Southern Medical University, Shenzhen, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Burn and Plastic Surgery, The People's Hospital of Baoan Shenzhen Affiliated to Southern Medical University, Shenzhen, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Bojie
authors.initials: B
authors.lastname: Lin
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address: doctormiao@aliyun.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D005260:Female; D018859:Hair Follicle / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D008810:Mice, Inbred C57BL; D009113:Muramidase / Q000494:pharmacology / Q000502:physiology*; D046509:Tissue Culture Techniques; D014738:Vibrissae / Q000201:enzymology* / Q000254:growth & development
chemical_list: D009113:Muramidase
keywords: Dermal papilla; Hair; Lysozyme; Vibrissae follicle