title: Characterization of desmoglein-3 epitope region peptides as synthetic antigens: analysis of their in vitro T cell stimulating efficacy, cytotoxicity, stability, and their conformational features.
abstract: Desmoglein-3 (Dsg3) adhesion protein is the main target of autoantibodies and autoreactive T cells in Pemphigus vulgaris (PV) autoimmune skin disorder. Several mapping studies of Dsg3 T cell epitope regions were performed, and based on those data, we designed and synthesized four peptide series corresponding to Dsg3 T cell epitope regions. Each peptide series consists of a 17mer full-length peptide (Dsg3/189-205, Dsg3/206-222, Dsg3/342-358, and Dsg3/761-777) and its N-terminally truncated derivatives, resulting in 15 peptides altogether. The peptides were prepared on solid phase and were chemically characterized. In order to establish a structure-activity relationship, the solution conformation of the synthetic peptides has been investigated using electronic circular dichroism spectroscopy. The in vitro T cell stimulating efficacy of the peptides has been determined on peripheral blood mononuclear cells isolated from whole blood of PV patients and also from healthy donors. After 20 h of stimulation, the interferon (IFN)-γ content of the supernatants was measured by enzyme-linked immunosorbent assay. In the in vitro conditions, peptides were stable and non-cytotoxic. The in vitro IFN-γ production profile of healthy donors and PV patients, induced by peptides as synthetic antigens, was markedly different. The most unambiguous differences were observed after stimulation with 17mer peptide Dsg3/342-358, and three truncated derivatives from two other peptide series, namely, peptides Dsg3/192-205, Dsg3/763-777, and Dsg3/764-777. Comparative analysis of in vitro activity and the capability of oligopeptides to form ordered or unordered secondary structure showed that peptides bearing high solvent sensibility and backbone flexibility were the most capable to distinguish between healthy and PV donors.
authors.affiliation: MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, PO Box 32 H-1518, Budapest, 112, Hungary.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Szabados
authors.affiliation: MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, PO Box 32 H-1518, Budapest, 112, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Uray
authors.affiliation: Laboratory for Chiroptical Structure Analysis, Institute of Chemistry, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Majer
authors.affiliation: Department of Dermatology, Venereology and Dermato-Oncology, Semmelweis University, Budapest, Hungary.
authors.forename: Pálma
authors.initials: P
authors.lastname: Silló
authors.affiliation: Department of Dermatology, Venereology and Dermato-Oncology, Semmelweis University, Budapest, Hungary.
authors.forename: Sarolta
authors.initials: S
authors.lastname: Kárpáti
authors.affiliation: MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, PO Box 32 H-1518, Budapest, 112, Hungary.|Department of Organic Chemistry, Institute of Chemistry, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Hudecz
authors.affiliation: MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, PO Box 32 H-1518, Budapest, 112, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Bősze
mesh_terms: D002851:Chromatography, High Pressure Liquid; D051184:Desmoglein 3 / Q000737:chemistry*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear; D010392:Pemphigus / Q000276:immunology; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry* / Q000276:immunology*
chemical_list: D051184:Desmoglein 3; D018984:Epitopes, T-Lymphocyte; D014614:Vaccines, Synthetic
keywords: ECD spectroscopy; desmoglein-3; solution conformation; synthetic T cell epitope peptides