title: Mechano growth factor E peptide promotes rat bone marrow-derived mesenchymal stem cell migration through CXCR4-ERK1/2.
abstract: Mechano growth factor (MGF) is a splicing variant of insulin-like growth factor 1 (IGF-1). The unique C-terminal E domain of MGF (MGF-E) makes it distinct from the other variants of IGF-1. Our previous work demonstrated that MGF-25E induces the migration of rat bone marrow-derived mesenchymal stem cells (rMSCs) by altering their mechanical properties, which is accompanied by the activation of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway. However, the relationship between ERK1/2 activation and the change in mechanical properties has not been illustrated. In the present study, we determined that MGF-25E induced the migration of rMSCs by modulating CXCR4 to activate the ERK1/2 pathway. The analysis of the Young's modulus and F-actin remodeling indicated that MGF-25E increased the stiffness and the F-actin polymerization of rMSCs through the activation of the CXCR4-ERK1/2 pathway. For the first time, this study clarified the signaling pathway that regulates the mechanical properties of rMSCs and is responsible for MGF-25E-promoted migration.
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , People's Republic of China.
authors.forename: Kewen
authors.initials: K
authors.lastname: Wu
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , People's Republic of China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , People's Republic of China.
authors.forename: Guanbin
authors.initials: G
authors.lastname: Song
mesh_terms: D000818:Animals; D001596:Benzylamines; D001854:Bone Marrow Cells / Q000502:physiology; D002465:Cell Movement; D002478:Cells, Cultured; D000080027:Cyclams; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology; D006571:Heterocyclic Compounds / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018625:Microscopy, Atomic Force; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D001596:Benzylamines; C486264:Cxcr4 protein, rat; D000080027:Cyclams; D005419:Flavonoids; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; C548904:insulin-like growth factor-1, rat; C519382:mechano-growth factor, rat; D007334:Insulin-Like Growth Factor I; D048049:Extracellular Signal-Regulated MAP Kinases; C088327:plerixafor; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
keywords: C-X-C chemokine receptor type 4; Cell stiffness; F-actin; extracellular signal-regulated kinase 1/2; mechano growth factor; rat bone marrow-derived mesenchymal stem cells