title: Differentiation of high-risk stage I and II colon tumors based on evaluation of CAV1 gene expression.
abstract: BACKGROUND
  Several molecular markers are currently being investigated for their prognostic or predictive value in colorectal cancer. One of the genes proposed, as a potential molecular marker in CRC is CAV1.
  
  
  METHODS
  The level of CAV1 expression was investigated in low-stage (I and II TNM) colon cancers using Real-Time PCR and immunohistochemistry.
  
  
  RESULTS
  The level of CAV1 expression increased in tumors characterized by greater depths of invasiveness. The CAV1 expression level detected in tumors with a depth of invasion at stage T4 was significantly higher compared to that in T2 (P = 0.01) and T3 (P = 0.003) lesions. The length of a patient's survival depended on CAV1 expression level; the 10-year survival rate for patients with elevated expression of CAV1 was ∼59% compared with 91% for patients with reduced or unchanged expression of CAV1 (P = 0.007). The overall survival rate of patients with T3 + T4 lesions was significantly lower (P = 0.006) for patients with tumor displaying elevated CAV1 expression compared with patients with reduced or unchanged CAV1 expression.
  
  
  CONCLUSIONS
  Evaluation of CAV1 expression offers valuable prognostic information for patients with colorectal cancer, and could be used to select patients with stage I or II disease, who are at increased risk of unfavorable outcomes.
authors.affiliation: Department of Molecular Medicine, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kitowska
authors.affiliation: Department of Molecular Medicine, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Wesserling
authors.affiliation: Department of Molecular Medicine, Medical University of Gdansk, Gdansk, Poland.|Central Bank of Tissue and Genetic Material, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Seroczynska
authors.affiliation: Departemnt of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szutowicz
authors.affiliation: Departemnt of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ronowska
authors.affiliation: Department of Pathomorphology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Peksa
authors.affiliation: Department of Molecular Medicine, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Pawelczyk
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C495540:CAV1 protein, human; D051242:Caveolin 1; D012333:RNA, Messenger
keywords: CAV1; KRAS; PTRF; colon cancer; prognostic factor