title: Gypsum-based biomaterials: Evaluation of physical and mechanical properties, cellular effects and its potential as a pulp liner.
abstract: This in vitro study aimed to evaluate setting time and compressive strength of gypsum-based chitosan biomaterials and its effect on proliferation of stem cells from human exfoliated deciduous teeth (SHED) and alkaline phosphatase (ALP) activity. Pure-GYP was mixed with water (2.5 g: 1.9 mL); Gyp-CHT was prepared with gypsum, chitosan, and water (2.5 g: 0.285 g: 1.9 mL). Cell viability and ALP activity were assessed at different periods. Data were analyzed using SPSS (p<0.05). The setting times were 2.7 min and 2.8 min for pure-GYP and Gyp-CHT, respectively. Significantly higher compressive strength was observed with Gyp-CHT. SHED treatments with both materials were not cytotoxic. ALP was consistently higher in the treated groups compared with the control. Cellular attachments were evident with SEM. Excellent cellular viability with pure-GYP and Gyp-CHT, as well as increased ALP activities, suggested the possibility of tertiary dentin formation. Further studies are necessary to evaluate the biomaterials for its pulp protective potentialities.
authors.affiliation: Dental Speciality Clinic, Hospital Miri.
authors.forename: Amy
authors.initials: A
authors.lastname: Low
authors.forename: Hamidah
authors.initials: H
authors.lastname: Mohd Yusof
authors.forename: Fazal
authors.initials: F
authors.lastname: Reza
authors.forename: Asma
authors.initials: A
authors.lastname: Abdullah Nurul
authors.forename: Shaminea
authors.initials: S
authors.lastname: Sritharan
authors.forename: Niswathul
authors.initials: N
authors.lastname: Haania Zain Ali
authors.forename: Hasan
authors.initials: H
authors.lastname: Subhi Azeez
authors.forename: Adam
authors.initials: A
authors.lastname: Husein
mesh_terms: D000469:Alkaline Phosphatase; D001672:Biocompatible Materials; D002133:Calcium Sulfate / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003764:Dental Materials / Q000737:chemistry*; D003782:Dental Pulp; D006801:Humans; D008422:Materials Testing; D055595:Mechanical Phenomena; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000187:drug effects*; D013499:Surface Properties; D014094:Tooth, Deciduous
chemical_list: D001672:Biocompatible Materials; D003764:Dental Materials; D000469:Alkaline Phosphatase; D002133:Calcium Sulfate