title: Anticholinesterase, antioxidant activity and phytochemical investigation into aqueous extracts from five species of Agrimonia genus.
abstract: Aqueous extracts of aerial flowering parts of five Agrimonia species (Rosaceae): Agrimonia coreana Nakai, Agrimonia japonica (Miq.) Koidz, Agrimonia procera Wallr., Agrimonia eupatoria L. and Agrimonia leucantha Kunze were investigated on their antioxidant activity, measured using five different methods; the best was the extract from A. procera with IC50 values from 6 to 29 μg/mL. All the extracts displayed inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) at the tested concentration of 100 μg/mL. We found the highest inhibition of cholinesterase in the extract of A. japonica with inhibition 70.4% for AChE and 79.8% for BuChE. These findings are statistically significant in comparison with those of other extracts (p < 0.001). The phytochemical analyses showed that the antioxidant activity of Agrimonia extracts can be affected especially by hexahydroxydiphenoyl (HHDP)-glucose and quercetin glycosides, and inhibition of cholinesterases by apigenin, luteolin and quercetin glycosides.
authors.affiliation: a Department of Natural Drugs, Faculty of Pharmacy , University of Veterinary and Pharmaceutical Sciences Brno , Palackého 1/3, Brno 61242 , The Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Kubínová
authors.affiliation: a Department of Natural Drugs, Faculty of Pharmacy , University of Veterinary and Pharmaceutical Sciences Brno , Palackého 1/3, Brno 61242 , The Czech Republic.
authors.forename: Emil
authors.initials: E
authors.lastname: Švajdlenka
authors.affiliation: a Department of Natural Drugs, Faculty of Pharmacy , University of Veterinary and Pharmaceutical Sciences Brno , Palackého 1/3, Brno 61242 , The Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Jankovská
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D031981:Agrimonia / Q000737:chemistry* / Q000145:classification; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D047311:Luteolin / Q000302:isolation & purification / Q000494:pharmacology; D064209:Phytochemicals / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D064209:Phytochemicals; D010936:Plant Extracts; D047310:Apigenin; D011794:Quercetin; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D047311:Luteolin
keywords: Agrimonia species; antioxidant activity; cholinesterase inhibition; flavonoids; polyphenols