title: Controlled antiseptic/eosin release from chitosan-based hydrogel modified fibrous substrates.
abstract: Fibers of cellulose networks were stably coated with N-methacrylate glycol chitosan (MGC) shells using subsequent steps of dip coating and photo-curing. The photo-crosslinked MGC-coated cellulose networks preserved their fibrous structure. A model hydrophilic antiseptic solution containing eosin, chloroxylenol and propylene glycol was incorporated into the shells to study the drug release dynamics. Detailed drug release mechanism into phosphate buffered saline (PBS) solutions from coated and pristine fibers loaded with the antiseptic was investigated. The results show that the MGC-coated cellulose fibers enable the controlled gradual release of the drug for four days, as opposed to fast, instantaneous release from eosin coated pristine fibers. This release behavior was found to affect the antibacterial efficiency of the fibrous cellulose sheets significantly against Staphylococcus aureus and Candida albicans. In the case of the MGC-eosin functionalized system the antibacterial efficiency was as high as 85% and 90%, respectively, while for the eosin coated pristine cellulose system the efficiency was negative, indicating bacterial proliferation. Furthermore, the MGC-eosin system was shown to be efficacious in a model of wound healing in mice, reducing the levels of various pro-inflammatory cytokines that modulate early inflammatory phase responses. The results demonstrate good potential of these coated fibers for wound dressing and healing applications. Due to its easy application on common passive commercial fibrous dressings such as gauzes and cotton fibers, the method can render them active dressings in a cost effective way.
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Romano
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Farouk
authors.initials: F
authors.lastname: Ayadi
authors.affiliation: Department of Chemistry, University College London, London, United Kingdom; The MRC/UCL Centre for Molecular and Medical Virology, University College London, London, United Kingdom.
authors.forename: Loris
authors.initials: L
authors.lastname: Rizzello
authors.affiliation: Drug Discovery and Development, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Summa
authors.affiliation: Drug Discovery and Development, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Bertorelli
authors.affiliation: Center for Biomolecular Nanotechnologies, Istituto Italiano di Tecnologia @UniLe, Via Barsanti 1, 730101 Arnesano, Lecce, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pompa
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy; Istituto Nazionale di Ottica, CNR, Via Moruzzi 1, 56124 Pisa, Italy.
authors.forename: Fernando
authors.initials: F
authors.lastname: Brandi
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. Electronic address: ilker.bayer@iit.it.
authors.forename: Ilker S
authors.initials: IS
authors.lastname: Bayer
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. Electronic address: athanassia.athanassiou@iit.it.
authors.forename: Athanassia
authors.initials: A
authors.lastname: Athanassiou
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D002460:Cell Line; D002482:Cellulose / Q000737:chemistry* / Q000648:ultrastructure; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000187:drug effects; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D003692:Delayed-Action Preparations; C118638:glycol-chitosan; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002482:Cellulose; D048271:Chitosan; D004801:Eosine Yellowish-(YS)
keywords: Antiseptic/eosin release; Chitosan biopolymer; Fibrous cellulose wound dressings