title: The Edible Red Alga Porphyra yezoensis Promotes Neuronal Survival and Cytoarchitecture in Primary Hippocampal Neurons.
abstract: The edible red alga Porphyra yezoensis is among the most popular marine algae and is of economic and medicinal importance. In the present study, the neurotrophic and neuroprotective activities of the ethanol extract of P. yezoensis (PYE) were investigated in primary cultures of hippocampal neurons. Results revealed that PYE significantly increased neurite outgrowth at an optimal concentration of 15 µg/mL. PYE dose-dependently increased viable cells, significantly accelerated the rate of neuronal differentiation in cultures, promoted axodendritic arborization, and eventually induced synaptogenesis. In addition to morphological development, PYE also promoted functional maturation as indicated by the staining of live cultures with FM 1-43. Moreover, PYE increased neuronal survivability, which was attributed to reduced apoptosis and its ROS scavenging activity. Taurine, a major organic acid in PYE (2.584/100 mg of dry PYE) promoted neurite outgrowth in a dose-dependent manner, and this promotion was suppressed by the taurine antagonist isethionic acid. The study indicates that PYE and its active component, taurine, facilitate neuronal development and maturation and have a neuroprotective effect.
authors.affiliation: Department of Biotechnology, Pukyong National University, Namku, Busan, 608-737, Republic of Korea.
authors.forename: Md
authors.initials: M
authors.lastname: Mohibbullah
authors.affiliation: Department of Anatomy, Dongguk University College of Medicine, Gyeongju, 780-714, Republic of Korea.
authors.forename: Mohammad Maqueshudul Haque
authors.initials: MM
authors.lastname: Bhuiyan
authors.affiliation: Department of Biotechnology, Pukyong National University, Namku, Busan, 608-737, Republic of Korea.|Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
authors.forename: Md Abdul
authors.initials: MA
authors.lastname: Hannan
authors.affiliation: Department of Biotechnology, Pukyong National University, Namku, Busan, 608-737, Republic of Korea.
authors.forename: Paulos
authors.initials: P
authors.lastname: Getachew
authors.affiliation: Department of Biotechnology, Pukyong National University, Namku, Busan, 608-737, Republic of Korea.
authors.forename: Yong-Ki
authors.initials: YK
authors.lastname: Hong
authors.affiliation: RIS Center, IACF, Silla University, Sasang-gu, Busan, 617-736, Republic of Korea.
authors.forename: Jae-Suk
authors.initials: JS
authors.lastname: Choi
authors.affiliation: RIS Center, IACF, Silla University, Sasang-gu, Busan, 617-736, Republic of Korea.|Department of Biological Science, Silla University, Sasang-gu, Busan, 617-736, Republic of Korea.
authors.forename: In Soon
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Department of Anatomy, Dongguk University College of Medicine, Gyeongju, 780-714, Republic of Korea. moonis@dongguk.ac.kr.
authors.forename: Il Soo
authors.initials: IS
authors.lastname: Moon
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006624:Hippocampus / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D044664:Porphyra / Q000737:chemistry*; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents
keywords: Hippocampal neuron; Neuronal development; Neuroprotection; Porphyra yezoensis; Synaptogenesis; Taurine