title: Zinc finger X-chromosomal protein (ZFX) is a significant prognostic indicator and promotes cellular malignant potential in gallbladder cancer.
abstract: The Zinc finger X-chromosomal protein (ZFX), a novel member of the Krueppel C2H2-type zinc finger protein family, has been implicated in multiple human cancers. However, the clinical significance of ZFX expression in gallbladder cancer (GBC) remains largely unknown. In this study, we focused on the clinical significance, biological function and mechanism of ZFX in GBC, and found that ZFX protein overexpression was frequently detected in GBC tissues. The expression of ZFX was significantly correlated with histological grade, perineural invasion, and margin status and lead to a significantly poorer prognosis in GBC patients(P <0.001). Furthermore, knockdown of ZFX result in significant inhibition of proliferation, migration, invasion and cause cell cycle arrest in GBC-SD cells, while over-expression of ZFX in NOZ shows the opposite results. Activation of PI3K/AKT pathway maybe the potential mechanism behind these effects. In conclusion, ZFX may serve as a oncogene and could be used as a potential prognostic marker and genetic treatment target for GBC patients.
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Weng
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Xu'an
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Maolan
authors.initials: M
authors.lastname: Li
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Wu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Wu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Yijian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Shibo
authors.initials: S
authors.lastname: Liu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Mu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Shu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Runfa
authors.initials: R
authors.lastname: Bao
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Lu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: a Department of General Surgery ; Xinhua hospital ; School of Medicine ; Shanghai Jiaotong University ; & Research Institute of Biliary Tract Disease Affiliated to School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P. R. China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011379:Prognosis; D016335:Zinc Fingers
chemical_list: D019869:Phosphatidylinositol 3-Kinases
keywords: Gallbladder cancer; Immunohistochemistry; PI3K/AKT pathway; RNA interference; Zinc finger X-chromosomal protein; cell cycle regulation; proliferation