title: Microarray-based IgE detection in tears of patients with vernal keratoconjunctivitis.
abstract: BACKGROUND
  A specific allergen sensitization can be demonstrated in approximately half of the vernal keratoconjunctivitis (VKC) patients by conventional allergic tests. The measurement of specific IgE in tears using a multiplex allergen microarray may offer advantages to identify local sensitization to a specific allergen.
  
  
  METHODS
  In spring-summer 2011, serum and tears samples were collected from 10 active VKC patients (three females, seven males) and 10 age-matched normal subjects. Skin prick test, symptoms score and full ophthalmological examination were performed. Specific serum and tear IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens.
  
  
  RESULTS
  Normal subjects resulted negative for the presence of specific IgE both in serum and in tears. Of the 10 VKC patients, six resulted positive to specific IgE in serum and/or tears. In three of these six patients, specific IgE was found positive only in tears. Cross-reactivity between specific markers was found in three patients. Grass, tree, mites, animal but also food allergen-specific IgE were found in tears. Conjunctival provocation test performed out of season confirmed the specific local conjunctival reactivity.
  
  
  CONCLUSIONS
  Multiple specific IgE measurements with single protein allergens using a microarray technique in tear samples are a useful, simple and non-invasive diagnostic tool. ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross- and co-sensitization to a large variety of allergen molecules. The presence of specific IgE only in tears of VKC patients reinforces the concept of possible local sensitization.
authors.affiliation: Ophthalmology Unit, Department of Neuroscience, University of Padova, Padova, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Leonardi
authors.affiliation: Private Practice Allergy and Clinical Immunology, Padova, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Borghesan
authors.affiliation: Department of Biomedical Sciences - DSB, University of Padova, Padova, taly.
authors.forename: Diego
authors.initials: D
authors.lastname: Faggian
authors.affiliation: Department of Biomedical Sciences - DSB, University of Padova, Padova, taly.
authors.forename: Mario
authors.initials: M
authors.lastname: Plebani
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D003233:Conjunctivitis, Allergic / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000378:metabolism*; D008297:Male; D040081:Protein Array Analysis; D013666:Tears / Q000276:immunology*
chemical_list: D000485:Allergens; D015415:Biomarkers; D007073:Immunoglobulin E
keywords: conjunctival provocation; microarray; tear IgE; vernal keratoconjunctivitis