title: Multi-omics profiling reveals that eating difficulties developed consecutively to artificial nutrition in the neonatal period are associated to specific saliva composition.
abstract: Prolonged enteral or parenteral nutrition in neonatal periods sometimes results in eating difficulties persisting for years, with reduced food intake through the oral route and thereby reduced stimulation of the oral cavity. Aiming at describing the consequences on oral physiology, saliva of 21 children with eating difficulties (ED) was compared to that of 23 healthy controls, using various omics and targeted methods. Overall, despite heterogeneity within the groups (age, medication etc.), the three spectral methods (MALDI-TOF, SELDI-TOF, (1)H NMR) allowed discriminating ED and controls, confirming that oral stimulation by food intake plays a role in shaping the composition of saliva. Saliva of ED patients exhibited a lower antioxidant status and lower levels of the salivary protease inhibitors cystatins. Other discriminant features (IgA1, dimethylamine) may relate to modified oral and/or intestinal microbial ecology. Finally, salivary profiles of ED patients were partly comparable to those of subjects with exacerbated gustatory sensitivities, in particular with reduced abundance of cystatin SN and higher abundance of zinc-alpha-2-glycoprotein. Whether this translates taste hypersensitivity and contributes to the eating difficulties deserves further attention.
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; INRA, UMR1324 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, UMR Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France. Electronic address: Martine.Morzel@dijon.inra.fr.
authors.forename: Martine
authors.initials: M
authors.lastname: Morzel
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; INRA, UMR1324 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, UMR Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Neyraud
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; INRA, UMR1324 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, UMR Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Brignot
authors.affiliation: IFR 100 Santé-STIC, CLIPP (Clinical and Innovation Proteomic Platform), F-21000 Dijon, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ducoroy
authors.affiliation: IFR 100 Santé-STIC, CLIPP (Clinical and Innovation Proteomic Platform), F-21000 Dijon, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Jeannin
authors.affiliation: IFR 100 Santé-STIC, CLIPP (Clinical and Innovation Proteomic Platform), F-21000 Dijon, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Lucchi
authors.affiliation: IFR 100 Santé-STIC, CLIPP (Clinical and Innovation Proteomic Platform), F-21000 Dijon, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Truntzer
authors.affiliation: INRA, UMR 1331 TOXALIM, Research Centre in Food Toxicology, F-31027 Toulouse, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Canlet
authors.affiliation: INRA, UMR 1331 TOXALIM, Research Centre in Food Toxicology, F-31027 Toulouse, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Tremblay-Franco
authors.affiliation: CHU Montpellier, Institute of Research in Biotherapy, Hôpital Saint Eloi, Laboratory of Biochemistry and Clinical Proteomics, INSERM U1040, F-34000 Montpellier France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
authors.affiliation: INSERM, CIC 1407, Hospices Civils de Lyon, CNRS, UMR5558, Université de Lyon, F-69000 Lyon, France.
authors.forename: Ségolène
authors.initials: S
authors.lastname: Gaillard
authors.affiliation: Université de Lyon, Claude Bernard Lyon 1, Faculté de Médecine Lyon-Est, INSERM U1060, CarMeN laboratory, Hospices Civils de Lyon, F-69003 Lyon, France.
authors.forename: Noël
authors.initials: N
authors.lastname: Peretti
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; INRA, UMR1324 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, UMR Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Feron
mesh_terms: D019959:Feeding and Eating Disorders of Childhood / Q000209:etiology* / Q000378:metabolism*; D005260:Female; D054794:Glycomics / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D010288:Parenteral Nutrition / Q000009:adverse effects*; D040901:Proteomics / Q000379:methods; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism*
keywords: Children; Feeding difficulties; Profiling; Saliva; Tube feeding