title: Mutation of FdC2 gene encoding a ferredoxin-like protein with C-terminal extension causes yellow-green leaf phenotype in rice.
abstract: Ferredoxins (Fds) are small iron-sulfur proteins that mediate electron transfer in a wide range of metabolic reactions. Besides Fds, there is a type of Fd-like proteins designated as FdC, which have conserved elements of Fds, but contain a significant C-terminal extension. So far, only two FdC genes of Arabidopsis (Arabidopsis thaliana) have been identified in higher plants and thus the functions of FdC proteins remain largely unknown. In this study, we isolated a yellow-green leaf mutant, 501ys, in rice (Oryza sativa). The mutant exhibited yellow-green leaf phenotype and reduced chlorophyll level. The phenotype of 501ys was caused by mutation of a gene on rice chromosome 3. Map-based cloning of this mutant resulted in identification of OsFdC2 gene (LOC_Os03g48040) showing high identity with Arabidopsis FdC2 gene (AT1G32550). OsFdC2 was expressed most abundantly in leaves and its encoded protein was targeted to the chloroplast. In 501ys mutant, a missense mutation was detected in DNA sequence of the gene, resulting in an amino acid change in the encoded protein. The mutant phenotype was rescued by introduction of the wild-type gene. Therefore, we successfully identified FdC2 gene via map-based cloning approach, and demonstrated that mutation of this gene caused yellow-green leaf phenotype in rice.
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Ma
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liao
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Fuliang
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Sun
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: xjdeng2006@aliyun.com.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Deng
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: prwang@sicau.edu.cn.
authors.forename: Pingrong
authors.initials: P
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005288:Ferredoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D056426:Genetic Loci; D054643:INDEL Mutation / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics; D020161:Physical Chromosome Mapping; D010860:Pigments, Biological / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D019655:Quantitative Trait, Heritable; D020539:Sequence Analysis, Protein; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D010860:Pigments, Biological; D010940:Plant Proteins
keywords: FdC; Ferredoxin; Map-based cloning; Rice; Yellow-green leaf mutant