title: Investigation into the interaction of methylparaben and erythromycin with human serum albumin using multispectroscopic methods.
abstract: In this paper, the interaction of methylparaben and erythromycin with human serum albumin (HSA) was studied for the first time using spectroscopic methods including Fourier transform infrared (FTIR) spectroscopy and UV absorption spectroscopy in combination with fluorescence quenching under physiological conditions. The binding parameters were evaluated using a fluorescence quenching method. Based on Förster's theory of non-radiation energy transfer, the binding average distance, r between the donor (HSA) and the acceptor (methylparaben and erythromycin) was evaluated. UV/vis absorption, FTIR, synchronous and 3D spectral results showed that the conformation of HSA was changed in the presence of methylparaben and erythromycin. The thermodynamic parameters were calculated according to the van't Hoff equation and are discussed. The effect of some biological metal ions and site probes on the binding of methylparaben and erythromycin to HSA were further examined.
authors.affiliation: Post Graduate Department of Studies in Chemistry, Karnatak University, Dharwad, 580 003, India.
authors.forename: Keerti M
authors.initials: KM
authors.lastname: Naik
authors.affiliation: Post Graduate Department of Studies in Chemistry, Karnatak University, Dharwad, 580 003, India.
authors.forename: Sharanappa T
authors.initials: ST
authors.lastname: Nandibewoor
mesh_terms: D004917:Erythromycin / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D010226:Parabens / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010226:Parabens; D012709:Serum Albumin; D004917:Erythromycin; C015358:methylparaben
keywords: erythromycin; human serum albumin; methylparaben; spectroscopy