title: Thermosensitive Ion Channel Activation in Single Neuronal Cells by Using Surface-Engineered Plasmonic Nanoparticles.
abstract: Controlling cell functions using external photoresponsive nanomaterials has enormous potential for the development of cell-engineering technologies and intractable disease therapies, but the former currently requires genetic modification of the target cells. We present a method using plasma-membrane-targeted gold nanorods (pm-AuNRs) prepared with a cationic protein/lipid complex to activate a thermosensitive cation channel, TRPV1, in intact neuronal cells. Highly localized photothermal heat generation mediated by the pm-AuNRs induced Ca(2+) influx solely by TRPV1 activation. In contrast, the use of previously reported cationic AuNRs that are coated with a conventional synthetic polymer also led to photoinduced Ca(2+) influx, but this influx resulted from membrane damage. Our method provides an optogenetic platform without the need for prior genetic engineering of the target cells and might be useful for novel TRPV1-targeted phototherapeutic approaches.
authors.affiliation: Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510 (Japan).
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Nakatsuji
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510 (Japan).
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Numata
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Sakyo-ku, Kyoto 606-8501 (Japan).
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Morone
authors.affiliation: Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501 (Japan).
authors.forename: Shuji
authors.initials: S
authors.lastname: Kaneko
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510 (Japan).
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Sakyo-ku, Kyoto 606-8501 (Japan).|Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510 (Japan).
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imahori
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Sakyo-ku, Kyoto 606-8501 (Japan). murakami@icems.kyoto-u.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murakami
mesh_terms: D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D060846:Cell Engineering; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D059010:Single-Cell Analysis; D013499:Surface Properties; D050916:TRPV Cation Channels / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D006046:Gold; D002118:Calcium
keywords: cell engineering; ion channels; nanoparticles; photothermal effects; surface chemistry