title: Pore orientation mediated control of mechanical behavior of scaffolds and its application in cartilage-mimetic scaffold design.
abstract: Scaffolds with aligned pores are being explored in musculoskeletal tissue engineering due to their inherent structural anisotropy. However, influence of their structure on mechanical behavior remains poorly understood. In this work, we elucidate this dependence using chitosan-gelatin based random and aligned scaffolds. For this, scaffolds with horizontally or vertically aligned pores were fabricated using unidirectional freezing technique. Random, horizontal and vertical scaffolds were characterized for their mechanical behavior under compressive, tensile and shear loading regimes. The results revealed conserved trends in compressive, tensile and shear moduli, with horizontal scaffolds showing the least moduli, vertical showing the highest and random showing intermediate. Further, these scaffolds demonstrated a highly viscoelastic behavior under cyclic compressive loading, with a pore orientation dependent relative energy dissipation. These results established that mechanical behavior of porous scaffolds can be modulated by varying pore orientation alone. This finding paved the way to recreate the structural and consequent mechanical anisotropy of articular cartilage tissue using zonally varied pore orientation in scaffolds. To this end, monolithic multizonal scaffolds were fabricated using a novel sequential unidirectional freezing technique. The superficial zone of this scaffold had horizontally aligned pores while the deep zone consisted of vertically aligned pores, with a transition zone between the two having randomly oriented pores. This depth-dependent pore architecture closely mimicked the collagen alignment of native articular cartilage which translated into similar depth-dependent mechanical anisotropy as well. A facile fabrication technique, biomimetic pore architecture and associated mechanical anisotropy make this multizonal scaffold a promising candidate for cartilage tissue engineering.
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, Kanpur 208016, Uttar Pradesh, India. Electronic address: adityaa@iitk.ac.in.
authors.forename: Aditya
authors.initials: A
authors.lastname: Arora
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, Kanpur 208016, Uttar Pradesh, India. Electronic address: anjaneykothari@gmail.com.
authors.forename: Anjaney
authors.initials: A
authors.lastname: Kothari
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, Kanpur 208016, Uttar Pradesh, India. Electronic address: dsk@iitk.ac.in.
authors.forename: Dhirendra S
authors.initials: DS
authors.lastname: Katti
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D032701:Biomimetics / Q000379:methods*; D002356:Cartilage / Q000166:cytology*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D005615:Freezing; D006041:Goats; D008422:Materials Testing; D055595:Mechanical Phenomena; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials
keywords: Anisotropic scaffolds; Cartilage tissue engineering; Mechanical properties; Multizonal scaffolds; Pore alignment; Unidirectional freezing