title: Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity.
abstract: OBJECTIVE
  Because of reproductive toxic effects of chemotherapy, researchers have taken some techniques to preserve fertility potential. The present study was designed to point out the potential role of spermatogonial stem cell (SSC) therapy in reversing cisplatin (CP)-induced testicular toxicity and restore the spermatogenesis.
  
  
  METHODS
  Sixty rats were randomly divided into three groups: group 1, control group; group 2, rats received CP in a dose of 7 mg/kg/day for 5 consecutive days; group 3, CP was injected at 7 mg/kg per day for 5 consecutive days, and, on the 6th day of the experiment, rats were treated with SSC. Forty days after receiving the last dose of CP, rats were euthanized under anesthesia; testes were collected, and gene expression using real-time polymerase chain reaction for P53, Bax, caspase 9 and cytochrome c, testicular histological findings and oxidative status were determined.
  
  
  RESULTS
  Administration of cisplatin caused significant increases in malondialdehyde levels, Bax and caspase 9 genes expression levels concomitant with significant decreases in anti-oxidant enzyme activities, p53 and cytochrome c gene expression levels, along with some histopathological lesions in testicular tissue. SCC attenuated the disturbance in oxidant/anti-oxidant status and testicular apoptosis; this is associated with improvements in the histopathological view of the testicular tissue.
  
  
  CONCLUSIONS
  The current study highlights evidence that the SCC has anti-oxidative and anti-apoptotic properties that could reverse CP-induced testicular toxicity, in addition to their role in spermatogenesis.
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt, Medical Laboratory Department, Faculty of Applied Medical Science, Taif University, Taif, KSA.
authors.forename: Yousri M
authors.initials: YM
authors.lastname: Hussein
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: randahussiny@yahoo.com.
authors.forename: Randa H
authors.initials: RH
authors.lastname: Mohamed
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Sally M
authors.initials: SM
authors.lastname: Shalaby
authors.affiliation: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt, Histology Department, Faculty of Oral Medicine and Dentistry, MTI University, Cairo, Egypt.
authors.forename: Manal R
authors.initials: MR
authors.lastname: Abd El-Haleem
authors.affiliation: Clinical Pharmacology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Dalia M
authors.initials: DM
authors.lastname: Abd El Motteleb
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002945:Cisplatin / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017208:Rats, Wistar; D013094:Spermatozoa / Q000187:drug effects; D033581:Stem Cell Transplantation / Q000379:methods*; D013737:Testis / Q000187:drug effects* / Q000473:pathology*
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002945:Cisplatin
keywords: anti-apoptotic; anti-oxidant; cisplatin; infertility; spermatogonial stem cell