title: Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris.
abstract: Pemphigus vulgaris (PV) is a life-long, potentially fatal IgG autoantibody-mediated blistering disease targeting mucocutaneous keratinocytes (KCs). PV patients develop pathogenic anti-desmoglein (Dsg) 3 ± 1 and antimitochondrial antibodies (AMA), but it remained unknown whether and how AMA enter KCs and why other cell types are not affected in PV. Therefore, we sought to elucidate mechanisms of cell entry, trafficking, and pathogenic action of AMA in PV. We found that PVIgGs associated with neonatal Fc receptor (FcRn) on the cell membrane, and the PVIgG-FcRn complexes entered KCs and reached mitochondria where they dissociated. The liberated AMA altered mitochondrial membrane potential, respiration, and ATP production and induced cytochrome c release, although the lack or inactivation of FcRn abolished the ability of PVIgG to reach and damage mitochondria and to cause detachment of KCs. The assays of mitochondrial functions and keratinocyte adhesion demonstrated that although the pathobiological effects of AMA on KCs are reversible, they become irreversible, leading to epidermal blistering (acantholysis), when AMA synergize with anti-Dsg antibodies. Thus, it appears that AMA enter a keratinocyte in a complex with FcRn, become liberated from the endosome in the cytosol, and are trafficked to the mitochondria, wherein they trigger pro-apoptotic events leading to shrinkage of basal KCs uniquely expressing FcRn in epidermis. During recovery, KCs extend their cytoplasmic aprons toward neighboring cells, but anti-Dsg antibodies prevent assembly of nascent desmosomes due to steric hindrance, thus rendering acantholysis irreversible. In conclusion, FcRn is a common acceptor protein for internalization of AMA and, perhaps, for PV autoantibodies to other intracellular antigens, and PV is a novel disease paradigm for investigating and elucidating the role of FcRn in this autoimmune disease and possibly other autoimmune diseases.
authors.affiliation: From the Irvine Diabetes Center, Department of Medicine, and.
authors.forename: Yumay
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Departments of Dermatology and.
authors.forename: Alex
authors.initials: A
authors.lastname: Chernyavsky
authors.affiliation: Research and Diagnostic Antibodies, Las Vegas, Nevada 89032.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Webber
authors.affiliation: Departments of Dermatology and Biological Chemistry, and the Institute for Immunology, University of California at Irvine, Irvine, California 92967 and sgrando@uci.edu.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Grando
authors.affiliation: From the Irvine Diabetes Center, Department of Medicine, and Biological Chemistry, and phwang@uci.edu.
authors.forename: Ping H
authors.initials: PH
authors.lastname: Wang
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000235:genetics / Q000276:immunology*; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051182:Desmogleins / Q000235:genetics / Q000276:immunology*; D011992:Endosomes / Q000235:genetics / Q000276:immunology / Q000473:pathology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D006801:Humans; D015603:Keratinocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D010392:Pemphigus / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D021381:Protein Transport / Q000235:genetics / Q000276:immunology; D011961:Receptors, Fc / Q000235:genetics / Q000276:immunology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D001323:Autoantibodies; D051182:Desmogleins; D015395:Histocompatibility Antigens Class I; D011961:Receptors, Fc; C100688:Fc receptor, neonatal
keywords: antibody; antigen; autoimmunity; keratinocyte; mitochondria; mitochondrial apoptosis; pemphigus; skin