title: From mesophilic to thermophilic digestion: the transitions of anaerobic bacterial, archaeal, and fungal community structures in sludge and manure samples.
abstract: The shift of microbial communities during a transition from mesophilic anaerobic digestion (MAD) to thermophilic anaerobic digestion (TAD) was characterized in two treatments. One treatment was inoculated with sludge and the other was inoculated with manure. In this study, methane was produced both in MAD and TAD, but TAD has slightly more methane produced than MAD. A broad phylogenetic spectrum of bacterial, archaeal, and fungal taxa at thermophilic conditions was detected. Coprothermobacter, Bacillus, Haloplasma, Clostridiisalibacter, Methanobacterium, Methanothermobacter, Saccharomycetales, Candida, Alternaria, Cladosporium, and Penicillium were found almost exclusively in TAD, suggesting their adaptation to thermophilic conditions and ecological roles in digesting the organic compounds. The characterization of the lesser-known fungal community revealed that fungi probably constituted an important portion of the overall community within TAD and contributed to this process by degrading complex organic compounds. The shift of the microbial communities between MAD and TAD implied that temperature drastically affected the microbial diversity in anaerobic digestion. In addition, the difference in microbial communities between sludge and manure indicated that different source of inoculum also affected the microbial diversity and community.
authors.affiliation: Departmentof Biochemistry and Microbiology, Rutgers, the State University of New Jersey, New Brunswick, NJ, 08901, USA.
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Environmental Science and Engineering, College of Natural Resource and Environment, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Yu
authors.affiliation: Departmentof Biochemistry and Microbiology, Rutgers, the State University of New Jersey, New Brunswick, NJ, 08901, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Louie
authors.affiliation: Departmentof Biochemistry and Microbiology, Rutgers, the State University of New Jersey, New Brunswick, NJ, 08901, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Departmentof Biochemistry and Microbiology, Rutgers, the State University of New Jersey, New Brunswick, NJ, 08901, USA.
authors.forename: Chengsheng
authors.initials: C
authors.lastname: Zhu
authors.affiliation: College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, 2999 North Renmin Road, Songjiang District, Shanghai, 201620, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xue
authors.affiliation: College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, 2999 North Renmin Road, Songjiang District, Shanghai, 201620, China. pingao@dhu.edu.cn.
authors.forename: Pin
authors.initials: P
authors.lastname: Gao
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000145:classification* / Q000302:isolation & purification / Q000528:radiation effects; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000302:isolation & purification / Q000528:radiation effects; D058448:Biota / Q000528:radiation effects*; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification / Q000528:radiation effects; D008372:Manure / Q000382:microbiology* / Q000528:radiation effects; D008697:Methane / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*
chemical_list: D008372:Manure; D012722:Sewage; D008697:Methane
keywords: Activated sludge; Anaerobic digestion; Manure; Microbial community; Temperature