title: Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate.
abstract: Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay. In this assay, free methotrexate and folic acid Au nanoparticles competed for human dihydrofolate reductase (hDHFR)-functionalized Au nanoparticles (Au NP). The hDHFR-functionalized Au NPs were immobilized on a small glass sensor inserted in a portable 4-channel LSPR reader. This allowed rapid (minutes) and sensitive (nanomolar range) measurement of methotrexate concentration by means of total internal reflection plasmonic spectroscopy. The large bathochromic shifts of the plasmon-coupling assay led to striking colour changes visible to the naked eye for methotrexate at clinically-relevant concentrations. The results demonstrate the potential for therapeutic drug monitoring of a widely used chemotherapy agent, as assessed with the naked eye.
authors.affiliation: Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca.
authors.forename: H
authors.initials: H
authors.lastname: Yockell-Lelièvre
authors.affiliation: Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca.
authors.forename: N
authors.initials: N
authors.lastname: Bukar
authors.affiliation: Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca.
authors.forename: J L
authors.initials: JL
authors.lastname: Toulouse
authors.affiliation: Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca and PROTEO.
authors.forename: J N
authors.initials: JN
authors.lastname: Pelletier
authors.affiliation: Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca and Centre for self-assembled chemical structures (CSACS).
authors.forename: J-F
authors.initials: JF
authors.lastname: Masson
mesh_terms: D003124:Colorimetry; D016903:Drug Monitoring / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D008727:Methotrexate / Q000032:analysis* / Q000494:pharmacology; D008958:Models, Molecular; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011487:Protein Conformation; D020349:Surface Plasmon Resonance / Q000379:methods*; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate