title: Afferent fibers involved in the bradykinin-induced cardiovascular reflexes from the ovary in rats.
abstract: Bleeding or rupture of the ovary often accompanies ovarian cysts and causes severe pain and autonomic responses such as hypotension. It would be expected that ovarian afferents contribute to cardiovascular responses induced by ovarian failure. The present study examined cardiovascular responses to noxious chemical stimulation of the ovary by bradykinin, an algesic substance released by tissue damage, and explored the role of ovarian afferents in the ovarian-cardiovascular responses in anesthetized rats. Non-pregnant adult rats were anesthetized with pentobarbital and artificially ventilated. The carotid artery was cannulated to monitor blood pressure and heart rate. Noxious chemical stimulation was achieved by applying a small piece of cotton soaked with bradykinin to the surface of the ovary for 30s. Application of bradykinin (10(-4) M) to the ovary decreased heart rate and blood pressure. These cardiovascular responses were not significantly influenced by severance of the vagal nerves or the superior ovarian nerve, but were abolished by severance of the ovarian nerve plexus (ONP). Application of bradykinin (10(-4) M) to the ovary evoked afferent activity of the ONP both in vivo and in vitro preparations. These results indicate that the decreases in heart rate and blood pressure following chemical noxious stimulation of the ovary with bradykinin are reflex responses, whose afferent nerve pathway is mainly through afferent fibers in the ONP.
authors.affiliation: Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173-0015, Japan. Electronic address: suchida@tmig.or.jp.
authors.forename: Sae
authors.initials: S
authors.lastname: Uchida
authors.affiliation: Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173-0015, Japan.
authors.forename: Fusako
authors.initials: F
authors.lastname: Kagitani
authors.affiliation: Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173-0015, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Hotta
mesh_terms: D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D001920:Bradykinin / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000187:drug effects / Q000294:innervation* / Q000502:physiology; D017208:Rats, Wistar; D012018:Reflex / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000295:instrumentation; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology; D014665:Vasodilator Agents / Q000008:administration & dosage
chemical_list: D014665:Vasodilator Agents; D001920:Bradykinin
keywords: Blood pressure; Heart rate; Ovarian afferent fibers; Rat; Reflex