title: Chronic Wound Dressings Based on Collagen-Mimetic Proteins.
abstract: Objective:Chronic wounds are projected to reach epidemic proportions due to the aging population and the increasing incidence of diabetes. There is a strong clinical need for an improved wound dressing that can balance wound moisture, promote cell migration and proliferation, and degrade at an appropriate rate to minimize the need for dressing changes.Approach:To this end, we have developed a bioactive, hydrogel microsphere wound dressing that incorporates a collagen-mimetic protein, Scl2GFPGER, to promote active wound healing. A redesigned Scl2GFPGER, engineered collagen (eColGFPGER), was created to reduce steric hindrance of integrin-binding motifs and increase overall stability of the triple helical backbone, thereby resulting in increased cell adhesion to substrates.Results:This study demonstrates the successful modification of the Scl2GFPGERprotein to eColGFPGER, which displayed enhanced stability and integrin interactions. Fabrication of hydrogel microspheres provided a matrix with adaptive moisture technology, and degradation rates have potential for use in human wounds.Innovation:This collagen-mimetic wound dressing was designed to permit controlled modulation of cellular interactions and degradation rate without impact on other physical properties. Its fabrication into uniform hydrogel microspheres provides a bioactive dressing that can readily conform to irregular wounds.Conclusion:Overall, this new eColGFPGERshows strong promise in the generation of bioactive hydrogels for wound healing as well as a variety of tissue scaffolds.
authors.affiliation: Department of Biomedical Engineering, Texas A&M University , College Station, Texas.
authors.forename: Stacy
authors.initials: S
authors.lastname: Cereceres
authors.affiliation: Department of Biomedical Engineering, Texas A&M University , College Station, Texas.
authors.forename: Tyler
authors.initials: T
authors.lastname: Touchet
authors.affiliation: Institute for Bioscience and Technology, Texas A&M Health Science Center , Houston, Texas.
authors.forename: Mary Beth
authors.initials: MB
authors.lastname: Browning
authors.affiliation: Department of Biomedical Engineering, Texas A&M University , College Station, Texas.
authors.forename: Clayton
authors.initials: C
authors.lastname: Smith
authors.affiliation: Institute for Bioscience and Technology, Texas A&M Health Science Center , Houston, Texas.
authors.forename: Jose
authors.initials: J
authors.lastname: Rivera
authors.affiliation: Institute for Bioscience and Technology, Texas A&M Health Science Center , Houston, Texas.
authors.forename: Magnus
authors.initials: M
authors.lastname: Höök
authors.affiliation: Department of Large Animal Clinical Sciences, Texas A&M University , College Station, Texas.
authors.forename: Canaan
authors.initials: C
authors.lastname: Whitfield-Cargile
authors.affiliation: Institute for Bioscience and Technology, Texas A&M Health Science Center , Houston, Texas.
authors.forename: Brooke
authors.initials: B
authors.lastname: Russell
authors.affiliation: Department of Biomedical Engineering, Texas A&M University , College Station, Texas.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Cosgriff-Hernandez