title: Label-free single-cell protein quantification using a drop-based mix-and-read system.
abstract: Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abbaspourrad
authors.affiliation: 1] School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA [2] Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang 110001, China.
authors.forename: Huidan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1] School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA [2] School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Naiwen
authors.initials: N
authors.lastname: Cui
authors.affiliation: New England Biolabs, Inc. 240 County Road, Ipswich, MA 01938, USA.
authors.forename: Haruichi
authors.initials: H
authors.lastname: Asahara
authors.affiliation: New England Biolabs, Inc. 240 County Road, Ipswich, MA 01938, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: New England Biolabs, Inc. 240 County Road, Ipswich, MA 01938, USA.
authors.forename: Dongxian
authors.initials: D
authors.lastname: Yue
authors.affiliation: 1] School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA [2] Department of Physics, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Stephan A
authors.initials: SA
authors.lastname: Koehler
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Lloyd W
authors.initials: LW
authors.lastname: Ung
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: John
authors.initials: J
authors.lastname: Heyman
authors.affiliation: School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Ren
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Roy
authors.initials: R
authors.lastname: Ziblat
authors.affiliation: New England Biolabs, Inc. 240 County Road, Ipswich, MA 01938, USA.
authors.forename: Shaorong
authors.initials: S
authors.lastname: Chong
authors.affiliation: 1] School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA [2] Department of Physics, Harvard University, Cambridge, MA 02138, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz