title: Development of an enzyme-linked immunosorbent assay for the monitoring and surveillance of antibodies to porcine epidemic diarrhea virus based on a recombinant membrane protein.
abstract: The recent dramatic increase in reported cases of porcine epidemic diarrhea (PED) in pig farms is a potential threat to the global swine industry. Therefore, the accurate diagnosis, serological monitoring, and surveillance of specific antibodies in pigs resulting from porcine epidemic diarrhea virus (PEDV) infection or vaccination would be essential in helping to control the spread of PED. We developed and validated an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant membrane (M) protein of PEDV. To detect PEDV antibodies in eight herds, 382 serum samples were collected from sows that had been immunized with a PED vaccine, and screened using the developed ELISA in parallel with a serum neutralization (SN) assay. Of the tested samples, 276 were positive for the presence of PEDV antibodies according to both assays, while 98 were negative. An excellent agreement between the ELISA and the SN assay was observed (kappa=0.947; 95% confidence interval=0.910-0.984; McNemar's test, P=0.727). No cross-reaction was detected for the developed ELISA with other coronaviruses or other common pig pathogens. The developed ELISA could be used for serological evaluation and indirect diagnosis of PED infection.
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: Jing-Hui
authors.initials: JH
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, People's Republic of China. Electronic address: yuzhuzuo@yahoo.com.
authors.forename: Yu-Zhu
authors.initials: YZ
authors.lastname: Zuo
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Gu
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
authors.forename: Jing-Mei
authors.initials: JM
authors.lastname: Di
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000276:immunology / Q000517:prevention & control; D000086862:Coronavirus M Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D062665:Epidemiological Monitoring; D009500:Neutralization Tests; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000276:immunology / Q000517:prevention & control; D014763:Viral Matrix Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000086862:Coronavirus M Proteins; C000706236:M protein, Porcine epidemic diarrhea virus; D014763:Viral Matrix Proteins; D014765:Viral Vaccines
keywords: ELISA; M protein; Porcine epidemic diarrhea virus (PEDV); Serum antibodies