title: Identification, molecular characterization and gene expression analysis of sox1a and sox1b genes in Japanese flounder, Paralichthys olivaceus.
abstract: The transcription factor, Sox1 has been implicated in neural determination and differentiation as well as in the maintenance of neural progenitor cell status in mammals. However, the molecular cloning and expression of sox1 gene in marine fish have not been reported yet. In this study, we first cloned and characterized the full-length cDNAs and the partial 5'-flanking regions of Paralichthys olivaceus sox1a (Posox1a) and sox1b (Posox1b). Phylogenetic, gene structure, and chromosome synteny analyses revealed that Posox1a and Posox1b were co-orthologs and homologous to mammalian Sox1. The promoter regions of Posox1a and Posox1b were also analyzed and several potential transcription factor (TF) binding sites were identified which might modulate gene expression. Quantitative real-time RT-PCR (qRT-PCR) results showed that Posox1a and Posox1b were consistently expressed during embryogenesis, with the highest level at the neurula stage. Tissue distribution analyses revealed that Posox1a and Posox1b were abundant in the adult brain. Moreover, Posox1a had a faster evolution rate and much higher expression levels than Posox1b. These results provide a foundation for further surveying the function of PoSox1 genes during Japanese flounder development and neurogenesis.
authors.affiliation: Center for Developmental Cardiology, Institute for Translational Medicine, College of Medicine, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China; College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Jinning
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China. Electronic address: wangxubo@ouc.edu.cn.
authors.forename: Xubo
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Marine Life Science, Ocean University of China, Key Laboratory of Marine Genetics and Breeding, Ministry of Education, 5 Yushan Road, Qingdao 266003, China. Electronic address: qzhang@ouc.edu.cn.
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D005432:Flounder / Q000196:embryology* / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D055495:Neurogenesis / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D020033:Protein Isoforms; D055748:SOXB1 Transcription Factors
keywords: Expression profile; Japanese flounder (Paralichthys olivaceus); Promoter analysis; sox1a; sox1b