title: [Effects of hydroxyl acetylated curcumin induced sonodynamic therapy on viability, apoptosis and necrosis of THP-1 macrophages].
abstract: OBJECTIVE
  We aim to investigate the sonodynamic effect induced by hydroxyl acetylated curcumin (HAC) on THP-1 macrophages.
  
  
  METHODS
  THP-1 derived macrophages (1 x 10(5) per milliliter) were cultured with HAC at a concentration of 5 µg/mL for 4 h and then exposed to pulse ultrasound treatment (0.5 W/cm2) for 5 min. Six hours later, cell viability analysis was performed with CCK-8 assay, apoptosis and necrosis analysis were detected with Annexin V/PI staining by flow cytometery.
  
  
  RESULTS
  The cell viability of THP-1 macrophage decreased significantly in the group treated with the combination of HAC and ultrasound (P < 0.01), and HAC-SDT induced both apoptosis and necrosis in THP-1 macrophages, the apoptotic rate was higher than the necrotic rate with appropriate conditions, the maximum apoptosis/necrosis ratio was detected in sonodynamic therapy (SDT) group (P < 0.01).
  
  
  CONCLUSIONS
  hAC-SDT was effective to induce THP-1 macrophages apoptosis.
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Yang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Liang
authors.forename: Cheng-hai
authors.initials: CH
authors.lastname: Peng
authors.forename: Jia-li
authors.initials: JL
authors.lastname: Cheng
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Ld-qing
authors.initials: LQ
authors.lastname: Weng
authors.forename: Zhi-tao
authors.initials: ZT
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D009336:Necrosis; D014465:Ultrasonics
chemical_list: D003474:Curcumin