title: Identification of beta-sitosterol and stigmasterol in Bambusa bambos (L.) Voss leaf extract using HPLC and its estrogenic effect in vitro.
abstract: Focus of the study is to identify a safe alternate to Hormone Replacement Therapy by identifying the presence of β-sitosterol and stigmasterol in the hydroalcoholic extract of Bambusa bambos using HPTLC and RP-HPLC-PDA; by evaluating the estrogenic effects of extract containing β-sitosterol and stigmasterol on the growth of MCF-7 cells in vitro. Plant material was identified by DNA sequencing analysis. Presence of β-sitosterol and stigmasterol was confirmed by HPTLC and direct RP-HPLC-PDA. Peaks with retention time about 19.13 and 21.16min were found to be stigmasterol and β-sitosterol in extract. Extract was not cytotoxic to MCF-7 cells in any of the dilutions. It induced cell proliferation and all the dilutions except <500μg/ml have significantly increased cell multiplication. 15.6, 31.2, 62.5 and 125μg/ml of HEBB have shown influence on the proliferation rates similar to the standard 17β-estradiol. The results suggest that HEBB might be used as a safe alternative to estrogen replacement therapies.
authors.affiliation: Interdisciplinary Institute of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: Sandhiya
authors.initials: S
authors.lastname: Sriraman
authors.affiliation: Interdisciplinary Institute of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: Ganesh Munuswamy
authors.initials: GM
authors.lastname: Ramanujam
authors.affiliation: Interdisciplinary Institute of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: MohanKumar
authors.initials: M
authors.lastname: Ramasamy
authors.affiliation: Interdisciplinary Institute of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamilnadu, India. Electronic address: gpdubey13@gmail.com.
authors.forename: Govind Prasad
authors.initials: GP
authors.lastname: Dubey
mesh_terms: D031723:Bambusa / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D020834:Ethnopharmacology; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D048789:Phytoestrogens / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012855:Sitosterols / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D013265:Stigmasterol / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D048789:Phytoestrogens; D010936:Plant Extracts; D012855:Sitosterols; C025473:gamma-sitosterol; D013265:Stigmasterol
keywords: Bambusa bambos; Estrogenic activity; HRT; MCF-7; Menopause; Phytosterol