title: Structure of GTP-specific succinyl-CoA synthetase in complex with CoA.
abstract: Pig GTP-specific succinyl-CoA synthetase is an αβ-heterodimer. The crystal structure of the complex with the substrate CoA was determined at 2.1 Å resolution. The structure shows CoA bound to the amino-terminal domain of the α-subunit, with the free thiol extending from the adenine portion into the site where the catalytic histidine residue resides.
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
authors.forename: Ji
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Malhi
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
authors.forename: Jan
authors.initials: J
authors.lastname: Deneke
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
authors.forename: Marie Elizabeth
authors.initials: ME
authors.lastname: Fraser
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006160:Guanosine Triphosphate / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D013389:Succinate-CoA Ligases / Q000737:chemistry* / Q000235:genetics; D013552:Swine
chemical_list: D000214:Acyl Coenzyme A; D021122:Protein Subunits; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; C012046:succinyl-coenzyme A; D013389:Succinate-CoA Ligases
keywords: ATP-grasp fold; ligase