title: Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis.
abstract: We evaluated the performance of the molecular lab-on-chip-based VerePLEX Biosystem for detection of multidrug-resistant tuberculosis (MDR-TB), obtaining a diagnostic accuracy of more than 97.8% compared to sequencing and MTBDRplus assay for Mycobacterium tuberculosis complex and rifampin and isoniazid resistance detection on clinical isolates and smear-positive specimens. The speed, user-friendly interface, and versatility make it suitable for routine laboratory use.
authors.affiliation: Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Cabibbe
authors.affiliation: Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Miotto
authors.affiliation: Servei de Microbiologia, Hospital Universitari de Bellvitge-IDIBELL, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Moure
authors.affiliation: Servei de Microbiologia, Hospital Universitari de Bellvitge-IDIBELL, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Alcaide
authors.affiliation: Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Feuerriegel
authors.affiliation: Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Pozzi
authors.affiliation: Clinical TB and HIV Group, Blizard Institute, Queen Mary University of London, London, United Kingdom Department of Infectious Diseases and Immunity, Imperial College London, London, United Kingdom.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Nikolayevskyy
authors.affiliation: Clinical TB and HIV Group, Blizard Institute, Queen Mary University of London, London, United Kingdom Department of Infectious Diseases and Immunity, Imperial College London, London, United Kingdom.
authors.forename: Francis
authors.initials: F
authors.lastname: Drobniewski
authors.affiliation: Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Niemann
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland Ifakara Health Institute, Bagamoyo, United Republic of Tanzania.
authors.forename: Klaus
authors.initials: K
authors.lastname: Reither
authors.affiliation: Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy cirillo.daniela@hsr.it.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Cirillo
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D056656:Lab-On-A-Chip Devices; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D012293:Rifampin / Q000494:pharmacology; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis*
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin