title: Identification and characterization of proliferative retinopathy-related long noncoding RNAs.
abstract: Proliferative vitreoretinopathy (PVR) is a serious complication of retinal detachment and vitreoretinal surgery, which can lead to severe vision reduction. Long non-coding RNAs (lncRNAs) play critical roles in many biological processes and disease development. We attempted to determine the role of lncRNAs in the setting of PVR. Microarray analysis revealed that 78 lncRNAs were abnormally expressed in the epiretinal membranes (ERMs) of PVR patients, including 48 up-regulated and 30 down-regulated lncRNA transcripts. We subsequently focus on one lncRNA, MALAT1, and investigated its expression pattern in the biofluid of PVR patients. MALAT1 was significantly up-regulated in the cellular and plasma fraction of peripheral blood in PVR patients. MALAT1 expression was obviously reduced after PVR operation. In vitro experiments revealed the role of MALAT1 in regulating RPE proliferation and migration, which is critical for ERMs formation. This study suggests that lncRNAs are the potential regulators of PVR pathology. MALAT1 is a potential prognostic indicator and a target for the diagnosis and gene therapy for PVR diseases.
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Rong-Mei
authors.initials: RM
authors.lastname: Zhou
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Xiao-Qun
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yao
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Sai-Nan
authors.initials: SN
authors.lastname: Chen
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China; Institute of Integrated Medicine, Nanjing Medical University, Nanjing, China. Electronic address: jqin710@vip.sina.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China; Institute of Integrated Medicine, Nanjing Medical University, Nanjing, China. Electronic address: yanbiao1982@hotmail.com.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics*; D018630:Vitreoretinopathy, Proliferative / Q000097:blood* / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: LncRNA; MALAT1; PVR; Peripheral blood; RPE