title: A new monoterpene glucoside and complete assignments of dihydroflavonols of Pulicaria jaubertii: potential cytotoxic and blood pressure lowering activity.
abstract: One new monoterpene glucoside and five dihydroflavonols were isolated for the first time from the aerial parts of Pulicaria jaubertii and identified as p-menthane-2-O-β-D-glucopyranoside [1], dihydroquercetin (taxifolin) [2], 7,3'-di-O-methyltaxifolin [3], 3'-O-methyltaxifolin [4], 7-O-methyltaxifolin (padmatin) [5] and 7-O-methyl-dihydrokampferol (7-O-methylaromadenderin) [6]. The structures of these compounds were unambiguously assigned on the basis of NMR spectroscopic data ((1)H, (13)C, DEPT, HSQC, HMBC) and MS analysis. 2D-NMR methods required revision of assignments of H-6 and H-8 for dihydroflavonol compounds. Possible cytotoxic activity as well as blood pressure (BP) lowering activity were tested. The alcoholic extract showed cytotoxic activity against prostate carcinoma (PC-3), breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG-2) human cell lines with IC50 19.1, 20.0 and 24.1 μg, respectively. The higher dose levels of the alcoholic extract significantly reduced normal BP of rats in a dose-dependent manner.
authors.affiliation: a Faculty of Pharmacy, Pharmacognosy Department , Al-Azhar University , Cairo , Egypt.
authors.forename: Ehab A
authors.initials: EA
authors.lastname: Ragab
authors.affiliation: b Faculty of Pharmacy, Pharmacology Department , Umm Al-Qura University , Makkah , Saudi Arabia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Raafat
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects*; D045744:Cell Line, Tumor; D044948:Flavonols / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D039821:Monoterpenes / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D052587:Pulicaria / Q000737:chemistry*; D051381:Rats
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044948:Flavonols; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts
keywords: Pulicaria jaubertii; blood pressure lowering effect; cytotoxic activity; phytoconstituents