title: Angiotensin1-7 protects cardiomyocytes from hypoxia/reoxygenation-induced oxidative stress by preventing ROS-associated mitochondrial dysfunction and activating the Akt signaling pathway.
abstract: Angiotensin1-7 (Ang1-7) is a biologically active member of the renin-angiotensin system, which has been reported to exhibit protective effect in myocardial ischemia reperfusion-induced injury. However, the molecular basis of this effect is not well understood. It has been proposed that oxidative stress-induced cardiomyocyte apoptosis is a major consequence of hypoxia/reoxygenation (H/R) injury. This study investigates the protective effect of Ang1-7 against H/R-induced oxidative stress in rat H9C2 cells. Our results showed that Ang1-7 (80nM) treatment significantly protected cells from H/R-induced oxidative injury via improving cell viability and reducing cell apoptosis. The protective effect of Ang1-7 was associated with the inhibition of ROS-associated mitochondrial dysfunction as well as the induction of Akt phosphorylation. These findings may significantly contribute to better understanding the protective effect of Ang1-7, particularly in hypoxia/reoxygenation-induced heart diseases and form the basis in the therapeutic development in treating cardiovascular diseases.
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China. Electronic address: lifen1126@outlook.com.
authors.forename: Fen
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Children's Medical Center Affiliated to Shanghai Jiao Tong University Medical College, Shanghai 200127, China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Huang
mesh_terms: D000803:Angiotensin I / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D002316:Cardiotonic Agents / Q000494:pharmacology*; D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival; D004353:Drug Evaluation, Preclinical; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D032383:Myocytes, Cardiac; D018384:Oxidative Stress; D010100:Oxygen / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002316:Cardiotonic Agents; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D000803:Angiotensin I; D051057:Proto-Oncogene Proteins c-akt; C118790:angiotensin I (1-7); D010100:Oxygen
keywords: Akt signaling pathway; Angiotensin1-7; Apoptosis; Hypoxia/reoxygenation; Mitochondrial dysfunction