title: Involvement of adrenoceptors, dopamine receptors and AMPA receptors in antidepressant-like action of 7-O-ethylfangchinoline in mice.
abstract: OBJECTIVE
  7-O-ethylfangchinoline (YH-200) is a bisbenzylisoquinoline derivative. The aim of this study was to investigate the antidepressant-like action and underlying mechanisms of YH-200 in mice.
  
  
  METHODS
  Mice were treated with YH-200 (15, 30, and 60 mg/kg, ig) or tetrandrine (30 and 60 mg/kg, ig) before conducting forced swimming test (FST), tail suspension test (TST), or open field test (OFT).
  
  
  RESULTS
  YH-200 (60 mg/kg) significantly decreased the immobility time in both FST and TST, and prolonged the latency to immobility in FST. YH-200 (60 mg/kg) was more potent than the natural bisbenzylisoquinoline alkaloid tetrandrine (60 mg/kg) in FST. Pretreatment with α1-adrenoceptor antagonist prazosin (1 mg/kg), β-adrenoceptor antagonist propranolol (2 mg/kg), dopamine D1/D5 receptor antagonist SCH23390 (0.05 mg/kg), dopamine D2/D3 receptor antagonist haloperidol (0.2 mg/kg) or AMPA receptor antagonist NBQX (10 mg/kg) prevented the antidepressant-like action of YH-200 (60 mg/kg) in FST. In contrast, pretreatment with α2 adrenoceptor antagonist yohimbine (1 mg/kg) augmented the antidepressant-like action of YH-200 (30 mg/kg) in FST. Chronic administration of YH-200 (30 and 60 mg/kg for 14 d) did not produce drug tolerance; instead its antidepressant-like action was strengthened. Chronic administration of YH-200 did not affect the body weight of mice compared to control mice.
  
  
  CONCLUSIONS
  YH-200 exerts its antidepressant-like action in mice via acting at multi-targets, including α1, α2 and β-adrenoceptors, D1/D5 and D2 /D3 receptors, as well as AMPA receptors.
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Zhao-fu
authors.initials: ZF
authors.lastname: Sheng
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Xiang-yu
authors.initials: XY
authors.lastname: Cui
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Su-ying
authors.initials: SY
authors.lastname: Cui
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Xue-qiong
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Sheng-jie
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Yuan-li
authors.initials: YL
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Xu
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Jin-zhi
authors.initials: JZ
authors.lastname: Song
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Zhi-ge
authors.initials: ZG
authors.lastname: Lin
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100191, China.
authors.forename: Yong-he
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003863:Depression / Q000188:drug therapy / Q000378:metabolism; D019417:Hindlimb Suspension; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018091:Receptors, AMPA / Q000378:metabolism*; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism*
chemical_list: D000928:Antidepressive Agents; D044182:Benzylisoquinolines; D018091:Receptors, AMPA; D011941:Receptors, Adrenergic; D011954:Receptors, Dopamine; C069407:7-O-ethyl fangchinoline