title: Enantioseparation of Timolol on a Novel β-Cyclodextrin Derivative Chiral Stationary Phase in HPLC.
abstract: A novel chiral stationary phase was prepared by bonding a novel β-cyclodextrin derivative on silica gel, and it was used for the separation of timolol in high efficiency liquid phase. In the reverse mode, the factors such as the proportion of chiral additives, flow rate, column temperature, repeatability and stability were investigated. The optimum chromatographic conditions are as follows: column temperature was 25°C, flow rate was 0.6 mL min(-1) and mobile phase was methanol-25 mM KH2PO4 (80/20, v/v). The chiral column has good reproducibility (Rs = 4.49, 4.51 and 4.40, respectively) and a certain degree of stability (Rs = 4.49, 3.01 and 0.72, respectively). This chiral stationary phase presented good chiral recognition performance toward timolol with good resolution (Rs = 4.49).
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China Key Laboratory of State Ministry of Education for Pharmaceutical Technology, Zhengzhou, Henan 450001, PR China jie_0822@163.com jie_0822@zzu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Qiuzheng
authors.initials: Q
authors.lastname: Du
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Pei
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013999:Timolol / Q000737:chemistry / Q000302:isolation & purification*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000319:Adrenergic beta-Antagonists; D047392:beta-Cyclodextrins; D013999:Timolol; C031215:betadex