title: Advanced Assay Monitoring APP-Carboxyl-Terminal Fragments as Markers of APP Processing in Alzheimer Disease Mouse Models.
abstract: The 99-amino-acid-long APP-carboxy-terminal fragment, named C99, is a membrane-bound peptide generated from the amyloid precursor protein (APP) by β-secretase cleavage and is the direct precursor of amyloid beta (Aβ). Here we describe a method for the quantification of C99. The amount of C99 is an indicative value of the amyloid pathology in an Alzheimer's disease (AD) model, and could be used as a marker to study AD progression in comprehensive experiments, including screening for new compounds and repurposing of drugs to treat AD.
authors.affiliation: Cell and Molecular Neuropharmacology, Neurosciences Division, Centre for Applied Medical Research, CIMA, University of Navarra, Av. Pio XII 55, Pamplona, 31008, Spain, agosta@unav.es.
authors.forename: Ana
authors.initials: A
authors.lastname: García-Osta
authors.forename: Mar
authors.initials: M
authors.lastname: Cuadrado-Tejedor
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods*; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D004586:Electrophoresis; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008567:Membranes, Artificial; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D008567:Membranes, Artificial; D010446:Peptide Fragments