title: The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats.
abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.
authors.affiliation: Medical Physiology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt ; Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt.
authors.forename: Radwa A
authors.initials: RA
authors.lastname: Mehanna
authors.affiliation: Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt ; Histology and Cell Biology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt.
authors.forename: Iman
authors.initials: I
authors.lastname: Nabil
authors.affiliation: Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt ; Histology and Cell Biology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt.
authors.forename: Noha
authors.initials: N
authors.lastname: Attia
authors.affiliation: Pathology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt.
authors.forename: Amany A
authors.initials: AA
authors.lastname: Bary
authors.affiliation: Medical Research Institute, Alexandria University, 71 Victor Emanuel Street, Smouha, Alexandria 21615, Egypt.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Razek
authors.affiliation: Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab, P.O. Box 21934, Alexandria, Egypt.
authors.forename: Tamer A E
authors.initials: TA
authors.lastname: Ahmed
authors.affiliation: Cell Culture Department, Medical Research Institute, Alexandria University, 71 Victor Emanuel Street, Smouha, Alexandria 21615, Egypt.
authors.forename: Fatma
authors.initials: F
authors.lastname: Elsayed
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001854:Bone Marrow Cells; D002908:Chronic Disease; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D017077:Culture Media, Conditioned; D015718:Fibrin Tissue Adhesive