title: Enantiomeric separation of 1,3-dimethylamylamine by capillary electrophoresis with indirect UV detection using a dual-selector system.
abstract: The CE method employing an indirect UV detection for the enantioseparation of 1,3-dimethylamylamine (DMAA), widely used in various preworkout and dietary supplements labeled as a constituent of geranium extract has been developed. The dual-selector system consisting of negatively charged sulfated α-CD (1.1% w/v) and sulfated β-CD (0.2% w/v) in 5 mM phosphate/Tris buffer (pH 3.0) containing the addition of 10 mM benzyltriethylammonium chloride (BTEAC) as the chromophoric additive was used for the enantiomeric separation of DMAA stereoisomers with the LODs in the range of 7.82-9.24 μg/mL. The method was partly validated and applied for the determination of the stereoisomeric composition of DMAA in commercial dietary supplements to verify the potential natural origin of DMAA.
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic.
authors.forename: Adam
authors.initials: A
authors.lastname: Přibylka
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Švidrnoch
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic.
authors.forename: Juraj
authors.initials: J
authors.lastname: Ševčík
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic.
authors.forename: Vítězslav
authors.initials: V
authors.lastname: Maier
mesh_terms: D000588:Amines / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D019587:Dietary Supplements / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D013237:Stereoisomerism; D014466:Ultraviolet Rays; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: C571979:1,3-dimethylamylamine; D000588:Amines; D002021:Buffers; D047392:beta-Cyclodextrins
keywords: 1,3-Dimethylamylamine; Capillary electrophoresis; Dual-selector system; Enantioseparation; Indirect UV detection