title: Porcine kidneys as a source of ECM scaffold for kidney regeneration.
abstract: OBJECTIVE
  To produce and examine decellularized kidney scaffolds from porcine as a platform for kidney regeneration research.
  
  
  METHODS
  Porcine kidneys were decellularized with sodium dodecyl sulfate solution and Triton X-100 after the blood was rinsed. Then the renal ECM scaffolds were examined for vascular imaging, histology to investigate the vascular patency, degree of decellularization.
  
  
  RESULTS
  Renal ECM scaffolds of porcine kidneys were successfully produced. Decellularized renal scaffolds retained intact microarchitecture including the renal vasculature and essential extracellular matrix components.
  
  
  CONCLUSIONS
  We have developed an excellent decellularization method that can be used in large organs. These scaffolds maintain their basic components, and show intact vasculature system. This represents a step toward development of a transplantable organ using tissue engineering techniques.
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Kidney Transplantation, The Second Hospital, Shandong University, China.
authors.forename: Shuangde
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Central Research Laboratory, The Second Hospital, Shandong University, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Central Research Laboratory, The Second Hospital, Shandong University, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Department of Central Research Laboratory, The Second Hospital, Shandong University, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Luan
authors.affiliation: Department of Central Research Laboratory, The Second Hospital, Shandong University, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Central Research Laboratory, The Second Hospital, Shandong University, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, China.
authors.forename: Xiaoshuai
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, China. Electronic address: zhaoshengtian@sdu.edu.cn.
authors.forename: Shengtian
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry* / Q000502:physiology; D012038:Regeneration; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: Acellular scaffolds; Decellularization; Kidney regeneration; Whole organ engineering