title: Halogenase Engineering for the Generation of New Natural Product Analogues.
abstract: Halogenases catalyze the incorporation of halogen atoms into organic molecules. Given the importance that halogenation has on the biological activity of small molecules, these enzymes have been subjected to intense engineering efforts to make them more suitable for biotechnology applications. The ability to biohalogenate complex molecules provides, in principle, the opportunity for rapid generation of a series of analogues with new or improved properties. Here we discuss the potential and limitations of using halogenases as biocatalysts, including recent advances in engineering halogenases to generate halogenated natural product analogues.
authors.affiliation: Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, Norfolk, NR4 7UH, UK.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Brown
authors.affiliation: Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, Norfolk, NR4 7UH, UK. Sarah.O'Connor@jic.ac.uk.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: O'Connor
mesh_terms: D055162:Biocatalysis; D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism*; D054879:Halogenation; D006846:Hydrocarbons, Halogenated / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D001688:Biological Products; D006846:Hydrocarbons, Halogenated; D010088:Oxidoreductases
keywords: biocatalysis; directed evolution; halogenases; metabolic engineering; natural products