title: High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.
abstract: We report an implemented molecular-typing-method based on HRMA to detect SNPs within MLST loci, characterizing 100 clinical MRSA and 11 control strains, representative of Italian clones. The results provide solid evidence that HRMT could be a fast, cost-effective and reliable alternative to MLST, for MRSA molecular epidemiology.
authors.affiliation: Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
authors.forename: Gino
authors.initials: G
authors.lastname: Mongelli
authors.affiliation: Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
authors.forename: Dafne
authors.initials: D
authors.lastname: Bongiorno
authors.affiliation: Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Agosta
authors.affiliation: Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Benvenuto
authors.affiliation: Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
authors.affiliation: Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy. Electronic address: f.campanile@unict.it.
authors.forename: Floriana
authors.initials: F
authors.lastname: Campanile
mesh_terms: D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D017720:Molecular Epidemiology / Q000379:methods*; D058889:Molecular Typing / Q000379:methods*; D058885:Multilocus Sequence Typing; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*
keywords: Cost and time reduction; High Resolution Melting Typing (HRMT); Methicillin-resistant Staphylococcus aureus (MRSA); Molecular-epidemiology; Single-nucleotide polymorphisms (SNPs)