title: In vitro Effects of Prostaglandin Analogs on Cultured Astrocytes Obtained from the Lamina Cribrosa.
abstract: To evaluate the effects of prostaglandin analogs (PGAs) on cell viability and apoptosis in cultured astrocytes obtained from the lamina cribrosa (LC) of the human optic nerve head (ONH).
  
  
  
  Astrocytes were cultured from LC samples obtained from human donor ONH and treated with three kinds of acid form of PGAs: latanoprost (LAT-A), tafluprost (TAF-A), and bimatoprost (BIM-A) (0.1, 1, 10, 50 and 100 ug/mL). Cell viability was assessed using the WST-1 assay. Cell apoptosis was measured using the deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay. Apoptotic protein expression was evaluated using western blot analysis.
  
  
  
  ONH astrocytes expressed FP receptor in western blot analysis. In the presence of 0.1 ug/mL of LAT-A, BIM-A, and TAF-A, the cell viability was 85%, 85% and 82%, respectively. WST-1 assay revealed about 50% of cell viability following treatment with 50 ug/mL of all PGAs. After exposing astrocytes to 10 ug/mL of each PGA for 24 hours, apoptotic cells were stained in TUNEL assay. Western blot analysis revealed that the PGAs up-regulated Bax (pro-apoptotic protein) and down-regulated Bcl-xL (anti-apoptotic protein) in the astrocytes.
  
  
  
  PGAs affected cell viability in cultured astrocytes obtained from human ONH LC. PGA treatment may induce apoptosis in ONH astrocytes.
authors.affiliation: a Biomedical Research Center , Seoul , Korea .
authors.forename: Jin A
authors.initials: JA
authors.lastname: Shin
authors.affiliation: b College of Medicine, University of Ulsan , Seoul , Korea and.
authors.forename: Nam Woo
authors.initials: NW
authors.lastname: Kim
authors.affiliation: a Biomedical Research Center , Seoul , Korea .
authors.forename: Soon-Suk
authors.initials: SS
authors.lastname: Kang
authors.affiliation: a Biomedical Research Center , Seoul , Korea .
authors.forename: Mi-Lyang
authors.initials: ML
authors.lastname: Kim
authors.affiliation: c Department of Ophthalmology , College of Medicine, Asan Medical Center, University of Ulsan , Seoul , Korea.
authors.forename: Kyung Rim
authors.initials: KR
authors.lastname: Sung
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D002470:Cell Survival; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D020287:In Situ Nick-End Labeling; D008875:Middle Aged; D009898:Optic Disk / Q000166:cytology* / Q000378:metabolism; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D011982:Receptors, Prostaglandin / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D011465:Prostaglandins, Synthetic; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor
keywords: Astrocyte; lamina cribrosa; optic nerve head; prostaglandin analog