title: Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin.
abstract: A molecularly imprinted polymer film was in situ polymerized on a carboxyl functionalized multi-walled carbon nanotubes modified glassy carbon electrode surface under room temperature. This technique provides a promising imprinting approach for protein in an aqueous solution using 3-(3-aminopropyl)-1-vinylimidazolium tetrafluoroborate ionic liquid as functional monomer, N, N'-methylenebisacrylamide as crossing linker, ammonium persulfate and N,N,N',N'-tetramethylethylenediamine as initiator, and bovine serum albumin (BSA) as template. The molecularly imprinted polymerized ionic liquid film shows enhanced accessibility, high specificity and sensitivity towards BSA. Electrochemical sensing performance of the imprinted sensor was thoroughly investigated using K3Fe[CN]6/K4Fe[CN]6 as electroactive probes. Under optimal conditions, the current difference before and after specific recognition of BSA was found linearly related to its concentration in the range from 1.50×10(-9) to 1.50×10(-6) mol L(-1). The detection limit was calculated to be 3.91×10(-10) mol L(-1) (S/N=3). The practical application of the imprinted sensor was demonstrated by determining BSA in liquid milk samples.
authors.affiliation: Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China; Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Yanying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Han
authors.affiliation: Chaozhou Quality and Measurement Supervision and Inspection Institute, Chaozhou 521011, China.
authors.forename: Guishen
authors.initials: G
authors.lastname: Liu
authors.affiliation: Chaozhou Quality and Measurement Supervision and Inspection Institute, Chaozhou 521011, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Hou
authors.affiliation: Chaozhou Quality and Measurement Supervision and Inspection Institute, Chaozhou 521011, China.
authors.forename: Yina
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: kbwu@mail.hust.edu.cn.
authors.forename: Kangbing
authors.initials: K
authors.lastname: Wu
authors.affiliation: Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China. Electronic address: lcychem@yahoo.com.
authors.forename: Chunya
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D055664:Electrochemical Techniques; D052578:Ionic Liquids; D057230:Limit of Detection; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon / Q000737:chemistry*; D058105:Polymerization; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*
chemical_list: D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; Electrochemical sensing; In-situ-polymerization; Ionic liquid; Molecularly imprinted