title: Concanavalin A immobilized magnetic poly(glycidyl methacrylate) beads for prostate specific antigen binding.
abstract: The aim of this study was to prepare Concanavalin A (Con A) immobilized magnetic poly(glycidyl methacrylate) (mPGMA) beads for prostate specific antigen (PSA) binding and to study binding capacities of the beads using lectin-glycoprotein interactions. Firstly, iron oxide nanoparticles were synthesized by co-precipitation method and then, beads were synthesized by dispersion polymerization in the presence of iron oxide nanoparticles. Con A molecules were both covalently immobilized onto the beads directly and through the spacer arm (1,6-diaminohexane-HDMA). The total PSA and free PSA binding onto the mPGMA-HDMA-Con A beads were higher than that of the mPGMA-Con A beads. Maximum PSA binding capacity was observed as 91.2 ng/g. Approximately 45% of the bound PSA was eluted by using 0.1 M mannose as elution agent. The mPGMA-HDMA-Con A beads could be reused without a remarkable decrease in the binding capacities after 5 binding-desorption cycles. Serum fractions were analyzed using SDS-PAGE. The mPGMA-HDMA-Con A beads could be useful for the detection of PSA and suggested as a model system for other glycoprotein biomarkers.
authors.affiliation: Department of Biology, Hacettepe University, Beytepe, Ankara, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Idil
authors.affiliation: Department of Biology, Hacettepe University, Beytepe, Ankara, Turkey.
authors.forename: Işık
authors.initials: I
authors.lastname: Perçin
authors.affiliation: Department of Chemistry, Hacettepe University, Beytepe, Ankara, Turkey.
authors.forename: Veyis
authors.initials: V
authors.lastname: Karakoç
authors.affiliation: Department of Chemistry, Hacettepe University, Beytepe, Ankara, Turkey.
authors.forename: Handan
authors.initials: H
authors.lastname: Yavuz
authors.affiliation: Department of Biology, Hacettepe University, Beytepe, Ankara, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Aksöz
authors.affiliation: Department of Chemistry, Hacettepe University, Beytepe, Ankara, Turkey. Electronic address: denizli@hacettepe.edu.tr.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D008297:Male; D011109:Polymethacrylic Acids / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011109:Polymethacrylic Acids; D003208:Concanavalin A; C042535:polyglycidyl methacrylate; D017430:Prostate-Specific Antigen
keywords: Concanavalin A; Prostate cancer; Prostate specific antigen; Spacer arm; mPGMA beads