title: Identification of two arginine kinase forms of endoparasitoid Leptomastix dactylopii venom by bottom up-sequence tag approach.
abstract: Leptomastix dactylopii (Howard) is an endoparasitoid wasp, natural enemy of mealybug Planococcus citri (Risso). Despite the acquired knowledge regarding this host-parasitoid interaction, only little information is available on the factors of parasitoid origin able to modulate the mealybug physiology. The major alteration observed in P. citri is a strong reduction in fecundity, which is evident soon after parasitization by L. dactylopii or venom injection in unparasitized hosts indicating that this proteinaceus secretion injected at the oviposition plays a key-role in host regulation. Protein identification of L. dactilopii venom has been limited by the lack of literature sources and public protein databases. Here, we identified two venom proteins by an integrated trascriptomic and proteomic approach. A custom-made transcriptomic database from the L. dactylopii venom glands was created by applying the high-throughput RNA sequencing approach. Two-dimensional gel electrophoresis (2DE) trypsinized protein spots were analyzed by high-resolution mass spectrometry (FTICRMS-12 T). The most abundant peptide ions were fragmented by collision induced dissociation and the obtained sequence tags were subjected to custom-made protein database searching. Two putative arginine kinases (full-length and truncated form) were identified. This is the first case in which both, truncated and full length arginine kinases, are identified in an endoparasitoid non-paralyzing venom.
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Labella
authors.affiliation: Department of Environmental Sciences, Research Unit Analytical BioGeoChemistry (BGC), Ingolstaedter Landstrasse, 85764, Neuherberg, Germany.
authors.forename: Basem
authors.initials: B
authors.lastname: Kanawati
authors.affiliation: Department of Entomology, Host Plant Adaptation, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745, Jena, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Vogel
authors.affiliation: Department of Environmental Sciences, Research Unit Analytical BioGeoChemistry (BGC), Ingolstaedter Landstrasse, 85764, Neuherberg, Germany.|Chair of Analytical Food Chemistry, Technische Universität München, Alte Akademie 10, D-85354, Freising-Weihenstephan, Germany.
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitt-Kopplin
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Laurino
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Bianco
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Falabella
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry*; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*; D014862:Wasp Venoms / Q000737:chemistry*; D014863:Wasps
chemical_list: D019476:Insect Proteins; D014862:Wasp Venoms; D001122:Arginine Kinase
keywords: arginine kinase; bottom up sequence-tag; high resolution mass spectrometry; parasitoid; venom