title: The cytoskeletal protein LASP-1 differentially regulates migratory activities of choriocarcinoma cells.
abstract: OBJECTIVE
  During healthy pregnancy, a distinct but limited invasion of trophoblast cells into the uterus occurs. In contrast, excessive trophoblast invasion is associated with placental choriocarcinoma (CC). Overexpression of the cytoskeletal protein LASP-1 was shown to contribute to cancer aggressiveness. Here, the yet unknown role of LASP-1 in CC cells is analysed.
  
  
  METHODS
  Expression of LASP-1 in human primary carcinoma was assessed by immunohistochemistry and confirmed in CC-derived cell lines by immunocytochemistry, RT-PCR and Western blot. After down-regulation of LASP-1 expression with specific si-RNA in CC-derived cell lines, migratory and proliferative activities were analysed by matrigel migration assay and WST-8 test.
  
  
  RESULTS
  LASP-1 expression was detected in human primary choriocarcinoma and in JEG-3, JAR and BeWo cells. Knock down of LASP-1 resulted in a decreased expression of LASP-1 protein in JEG-3 and JAR cells accompanied by a diminished migration and a decreased proliferative activity of these two cell lines. Knockdown of LASP-1 in BeWo cells failed. In consequence, migratory function and proliferation was unaffected.
  
  
  CONCLUSIONS
  This is the first study describing LASP-1 expression in CC cells. Detecting an affection of migratory processes after LASP-1 silencing, we propose that LASP-1 could impact on metastasis of CC cells.
authors.affiliation: Endokrinologikum Hamburg, Lornsenstraße 4-6, 22767, Hamburg, Germany. sabine.segerer@endokrinologikum.com.
authors.forename: S E
authors.initials: SE
authors.lastname: Segerer
authors.affiliation: Department of Obstetrics and Gynaecology, University of Wuerzburg, Josef-Schneider-Straße 4, 97080, Würzburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Bartmann
authors.affiliation: Clinical Centre of Nuernberg-South, Childrens Hospital, Breslauer Str. 201, 90471, Nuernberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kaspar
authors.affiliation: Institute for Virology and Immunobiology, University of Wuerzburg, Versbacherstraße 7, 97078, Würzburg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Müller
authors.affiliation: Department of Obstetrics and Gynaecology, University of Wuerzburg, Josef-Schneider-Straße 4, 97080, Würzburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kapp
authors.affiliation: Institute of Clinical Biochemistry, University of Wuerzburg, Grombuehlstrasse 12, 97080, Würzburg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Butt
authors.affiliation: Department of Obstetrics and Gynaecology, University of Wuerzburg, Josef-Schneider-Straße 4, 97080, Würzburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Kämmerer
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000235:genetics* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: Choriocarcinoma; Cytoskeleton; Migration; Proliferation