title: Imaging Cell Cycle Phases and Transitions of Living Cells from Yeast to Woman.
abstract: The eukaryotic cell cycle is comprised of different phases that take place sequentially once, and normally only once, every division cycle. Such a dynamic process is best viewed in real time in living dividing cells. The insights that can be gained from such methods are considerably larger than any alternative technique that only generates snapshots. A great number of studies can gain from live cell imaging; however this method often feels somewhat intimidating to the novice. The purpose of this chapter is to demonstrate that imaging cell cycle phases in living cells from yeast to human is relatively easy and can be performed with equipment that is available in most research institutes. We present the different approaches, review different types of reporters, and discuss in depth all the aspects to be considered to obtain optimal results. We also describe our latest cell cycle markers, which afford unprecedented "sub"-phase temporal resolution.
authors.affiliation: The Department of Genetics and The Bio-Imaging Unit, The Hebrew University of Jerusalem, Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Hadas
authors.initials: H
authors.lastname: Segev
authors.forename: Drora
authors.initials: D
authors.lastname: Zenvirth
authors.forename: Kobi J
authors.initials: KJ
authors.lastname: Simpson-Lavy
authors.forename: Naomi
authors.initials: N
authors.lastname: Melamed-Book
authors.forename: Michael
authors.initials: M
authors.lastname: Brandeis
mesh_terms: D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006801:Humans; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D004718:Saccharomycetales / Q000166:cytology*