title: Crosstalk between SOXB1 proteins and WNT/β-catenin signaling in NT2/D1 cells.
abstract: During early vertebrate embryogenesis, the expression of SOXB1 proteins is precisely regulated by a number of different mechanisms, including Wnt/β-catenin signaling. This is essential for controlling the balance between stemness and differentiation in embryonic stem cells. In the present study, we analyzed the molecular mechanism of LiCl action in NT2/D1 cells and examined the crosstalk between SOXB1 proteins and Wnt signaling in this model system. We have shown that LiCl increases β-catenin level, induces its translocation to the nucleus and consequently up-regulates β-catenin/Tcf-dependent transcription in NT2/D1 cells. Our results also suggest that LiCl treatment leads to increased expression of SOX2 and SOX3 proteins in NT2/D1 cells through activation of canonical Wnt signaling. Finally, we have detected a negative feedback loop between β-catenin and SOX2 expression in NT2/D1 cells. Since β-catenin and SOX2 have been linked to processes of self-renewal and pluripotency, our results have implications for future research on the maintenance of stemness and lineage commitment of embryonic stem cells.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia. mojsin@imgge.bg.ac.rs.
authors.forename: Marija
authors.initials: M
authors.lastname: Mojsin
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia.
authors.forename: Vladanka
authors.initials: V
authors.lastname: Topalovic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia.
authors.forename: Jelena Marjanovic
authors.initials: JM
authors.lastname: Vicentic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Schwirtlich
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Stanisavljevic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Drakulic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010, Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Stevanovic
mesh_terms: D006801:Humans; D018021:Lithium Chloride / Q000737:chemistry; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D055748:SOXB1 Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin; D018021:Lithium Chloride
keywords: LiCl; NT2/D1 cells; SOXB1; Wnt signaling; β-Catenin