title: Accelerating peroxidase mimicking nanozymes using DNA.
abstract: DNA-capped iron oxide nanoparticles are nearly 10-fold more active as a peroxidase mimic for TMB oxidation than naked nanoparticles. To understand the mechanism, the effect of DNA length and sequence is systematically studied, and other types of polymers are also compared. This rate enhancement is more obvious with longer DNA and, in particular, poly-cytosine. Among the various polymer coatings tested, DNA offers the highest rate enhancement. A similar acceleration is also observed for nanoceria. On the other hand, when the positively charged TMB substrate is replaced by the negatively charged ABTS, DNA inhibits oxidation. Therefore, the negatively charged phosphate backbone and bases of DNA can increase TMB binding by the iron oxide nanoparticles, thus facilitating the oxidation reaction in the presence of hydrogen peroxide.
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.
authors.forename: Biwu
authors.initials: B
authors.lastname: Liu
authors.forename: Juewen
authors.initials: J
authors.lastname: Liu
mesh_terms: D001560:Benzidines / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: C000594526:2,2-azinobis-3-ethylbenxothiazoline-6-sulfonic acid; D001560:Benzidines; D001672:Biocompatible Materials; D005290:Ferric Compounds; D013451:Sulfonic Acids; D013844:Thiazoles; C000499:ferric oxide; C021758:3,3',5,5'-tetramethylbenzidine; D004247:DNA; D010544:Peroxidases