title: [PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405].
abstract: It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6-phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol.min-1.mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol.min-1.mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol.min-1.mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714-803 nmol.min-1.mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP-carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glycoand aminolipids, respectively.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: T A
authors.initials: TA
authors.lastname: Shevchuk
authors.forename: K A
authors.initials: KA
authors.lastname: Beregova
authors.forename: N V
authors.initials: NV
authors.lastname: Kudrya
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000502:physiology; D004098:Dihydroxyacetone / Q000378:metabolism; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D005943:Gluconeogenesis / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D018086:Glutamate Dehydrogenase (NADP+) / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D007656:Ketoglutaric Acids / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000378:metabolism; D009615:Nocardia / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000502:physiology; D010728:Phosphoenolpyruvate / Q000378:metabolism; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005942:Gluconates; D005994:Glycerophosphates; D007656:Ketoglutaric Acids; D004099:Dihydroxyacetone Phosphate; D010728:Phosphoenolpyruvate; C029620:alpha-glycerophosphoric acid; D010734:Phosphogluconate Dehydrogenase; D042843:Glucose 1-Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D007658:Ketone Oxidoreductases; C023096:2-oxoglutarate synthase; D018086:Glutamate Dehydrogenase (NADP+); D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D010730:Phosphoenolpyruvate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D005947:Glucose; D004098:Dihydroxyacetone; D005990:Glycerol; C030691:gluconic acid; C008884:6-phosphogluconic acid