title: Changes of the expressions of multiple metabolism genes in rat pancreatic islets after ventromedial hypothalamic lesioning.
abstract: It was recently reported that ventromedial hypothalamic lesions change the expression of cell proliferation-related genes and morphology-related genes in rat pancreatic islets. This study has examined how gene families involved in metabolism are regulated in rat pancreatic islets after VMH lesions formation. Total pancreatic islets RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH-lesioned rats were investigated using DNA microarray and real-time polymerase chain reaction. The VMH lesions regulated the genes that are involved in functions related to metabolism in the pancreas islets. Real-time polymerase chain reaction also confirmed that gene expressions of arachidonate 15-lipoxygenase (Alox15) was up-regulated and pancreatic lipase (Pnlip) was downregulated at day 3 after the VMH lesions. Ventromedial hypothalamic lesions may change the expression of multiple metabolism genes in rat pancreatic islets.
authors.affiliation: Division of Modern Medical Technology, Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Kure, 737-0023, Japan. Electronic address: kibat@kure-nh.go.jp.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kiba
mesh_terms: D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007515:Islets of Langerhans / Q000378:metabolism*; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017207:Rats, Sprague-Dawley; D059467:Transcriptome; D014697:Ventromedial Hypothalamic Nucleus / Q000378:metabolism* / Q000601:surgery
chemical_list: D001093:Arachidonate 15-Lipoxygenase; D008049:Lipase
keywords: Metabolism genes; Pancreatic islets; VMH lesions