title: Whole-body vibration improves the anti-inflammatory status in elderly subjects through toll-like receptor 2 and 4 signaling pathways.
abstract: Regular physical exercise has anti-inflammatory effects in elderly subjects. Yet, the inflammatory responses after whole body vibration (WBV) training, a popular exercise paradigm for the elderly, remain to be elucidated. This study assessed the effects of WBV training on the inflammatory response associated with toll-like receptors (TLRs) signaling pathways. Twenty-eight subjects were randomized to a training group (TG) or a control group (CG). TG followed an 8-week WBV training program. Blood samples were obtained before and after the training period in both groups. Peripheral blood mononuclear cells were isolated, and mRNA and protein levels of makers involved in the TLR2/TLR4 myeloid differentiation primary response gen 88 (MyD88) and TIR domain-containing adaptor inducing interferon (TRIF)-dependent pathways were analyzed. Plasma TNFα and C-reactive protein levels were also assessed. The WBV program reduced protein expression of TLR2, TLR4, MyD88, p65, TRIF and heat shock protein (HSP) 60, while HSP70 content increased. IL-10 mRNA level and protein concentration were upregulated, and TNFα protein content decreased, after WBV training. Plasma concentration of C-reactive protein and TNFα decreased in the TG. The current data suggest WBV may improve the anti-inflammatory status of elderly subjects through an attenuation of MyD88- and TRIF-dependent TLRs signaling pathways.
authors.affiliation: Division of Clinical Translational Science, Georgia Prevention Institute, Department of Pediatrics, Georgia Regents University, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Rodriguez-Miguelez
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Fernandez-Gonzalo
authors.affiliation: Institute of Biomedicine (IBIOMED), University of León, León, Spain.
authors.forename: Pilar S
authors.initials: PS
authors.lastname: Collado
authors.affiliation: Institute of Biomedicine (IBIOMED), University of León, León, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Almar
authors.affiliation: Institute of Biomedicine (IBIOMED), University of León, León, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Martinez-Florez
authors.affiliation: Institute of Biomedicine (IBIOMED), University of León, León, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: de Paz
authors.affiliation: Institute of Biomedicine (IBIOMED), University of León, León, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: González-Gallego
authors.affiliation: Institute of Biomedicine (IBIOMED), University of León, León, Spain. Electronic address: mj.cuevas@unileon.es.
authors.forename: María J
authors.initials: MJ
authors.lastname: Cuevas
mesh_terms: D000368:Aged; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014732:Vibration
chemical_list: D018836:Inflammation Mediators; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
keywords: Elderly; Heat shock proteins; Inflammation; Toll-like receptors; Whole body vibration