title: Cell Synchronization of Mouse Embryonic Fibroblasts.
abstract: A fundamental need in the analysis of the cell cycle is the ability to isolate relatively homogeneous populations of cells in different phases. This is complicated by the variable proliferative properties and responses to synchronizing methods of different cancer-derived cell lines. Paradoxically, cell lines with genetic defects in cell cycle control are sometimes chosen because they are amenable to chemical synchronization. Embryonic fibroblasts from mice present the opportunity to study the effects of defined genetic modifications on a normal cell cycle. However, synchronization of these cells has often been challenging. In this chapter we outline three basic protocols for isolating mouse fibroblasts at the G1-to-S-phase transition, in S phase, and during mitosis.
authors.affiliation: London Regional Cancer Program, Department of Biochemistry, Children's Health Research Institute, Western University, London, ON, Canada.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Thwaites
authors.forename: Courtney H
authors.initials: CH
authors.lastname: Coschi
authors.forename: Christian E
authors.initials: CE
authors.lastname: Isaac
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Dick
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002469:Cell Separation / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D016193:G1 Phase; D051379:Mice; D008938:Mitosis; D011247:Pregnancy; D016196:S Phase