title: Smashing Tissue Extraction of Five Lignans From the Fruit of Schisandra chinensis.
abstract: Schisandra chinensis is one of the most famous herbal medicines in China, Korea and Japan. It has been widely used as a tonic, sedative, anti-aging and astringent agent. Lignans are one of its main bioactive components. The classical methods for extracting lignans, however, were tedious and energy-consuming. With the aim to develop an effective extraction method of lignans, the smashing tissue extraction (STE) technique was adopted and optimized in this study. Extraction conditions of STE have been optimized by the response surface methodology based on the Box-Behnken design. Results showed that 75% aqueous ethanol was the optimal extraction solvent, and the other optimal conditions were as follows: extraction voltage of 180 V, extraction time of 1 min, solid-liquid ratio of 1 : 19 and sample particle size of 120 mesh. Under these optimized conditions, the total content of the five lignans (Schisandrol A, Schisantherin A, Deoxyschisandrin, Schisandrin B and Schisandrin C) in S. chinensis collected from Baishan City located in the northeast of China was 13.89 ± 0.014 mg/g, which was well matched with the value predicted by the model. Other techniques, including heat reflux, Soxhlet, ultrasonic-assisted and microwave-assisted extraction, were further compared. Results suggested that STE had the highest extraction efficiency of lignans with the shortest time. It indicates that the approach proposed in this study is a simple and efficient technique for the extraction of lignans in S. chinensis.
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Laboratory of Test Center, Jilin Agricultural Science and Technology College, Jilin 132101, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Song
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China yanghjm@163.com 844503608@qq.com.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhou
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification*; D008872:Microwaves; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D017705:Lignans; D010936:Plant Extracts