title: Molecular cloning, expression profiles and characterization of a glutathione reductase in Hevea brasiliensis.
abstract: Glutathione reductase (GR; EC 1.8.1.7) is an important oxidoreductase that can protect organisms against various oxidative stresses. In this study, a new GR gene, named as HbGR2, was isolated from Hevea brasiliensis. The HbGR2 cDNA contained a 1674-bp open reading frame encoding 557 amino acids and the deduced HbGR2 protein showed high identities to the chloroplastic GRs from other plant species. HbGR2 was localized in the chloroplasts of tobacco mesophyll protoplasts. The cis-acting regulatory elements related to stress or hormone responses were predicted in the promoter region of HbGR2. The results from real-time RT-PCR analyses showed that HbGR2 was expressed throughout different tissues and developmental stages of leaves. Besides being related to tapping panel dryness (TPD), HbGR2 was regulated by several treatments including ethephon (ET), methyl jasmonate (MeJA), drought, low temperature, high salt, wounding and hydrogen peroxide (H2O2). The Escherichia coli (E. coli) cells overexpressing HbGR2 markedly increased their tolerance and survival at high concentrations of H2O2, suggesting that HbGR2 might play an important role in oxidative stress response in Hevea brasiliensis.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China. Electronic address: zizip@163.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China; College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: 760470903@qq.com.
authors.forename: Manman
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China. Electronic address: liuhui8645@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China. Electronic address: xwangyk@126.com.
authors.forename: Yuekun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China. Electronic address: djli.rricatas@gmail.com.
authors.forename: Dejun
authors.initials: D
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005980:Glutathione Reductase / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D028482:Hevea / Q000201:enzymology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D011993:Recombinant Fusion Proteins; D006861:Hydrogen Peroxide; D005980:Glutathione Reductase
keywords: Expression profiles; Glutathione reductase; H(2)O(2) tolerance; Hevea brasiliensis; Oxidative stress; Subcellular localization