title: The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation.
abstract: OBJECTIVE
  Reducing the ischemic damage from free radicals that is inflicted on ovarian tissue is critical for successful ovarian tissue transplantation. Polyethylene glycol-superoxide dismutase (PEG-SOD) is mimetic of superoxide dismutase (SOD) and powerful free radical scavenger acts by reducing superoxide anions. The objective of study was to evaluate effects of PEG-SOD on mouse ovarian tissues in in vitro culture and in autotransplantation.
  
  
  METHODS
  Ovaries were collected and randomly divided into four groups that received different doses of PEG-SOD. To assess effects of PEG-SOD on in vitro cultures, four different doses of PEG-SOD were applied to in vitro culture media during in vitro culturing following ovarian tissue vitrification and warming. To evaluate effects of PEG-SOD on ovarian tissue transplantation, four different doses of PEG-SOD were applied for 2, 7, and 21 days to mice following vitrified-warmed mouse ovarian tissue autotransplantation.
  
  
  RESULTS
  The percentage of primordial follicles was maintained at the highest dose of PEG-SOD for 2 h in vitro, and there was a significant decrease in the percentage of apoptotic follicles at 2 h, but not at later time points. The highest dose of PEG-SOD also maintained primordial, primary, and secondary follicles 2 days post-transplantation, but only primordial follicles were maintained up to 21 days after transplantation.
  
  
  CONCLUSIONS
  PEG-SOD is protective mainly toward primordial follicles only for a short interval in vitro, presumably via antioxidant effects. PEG-SOD may be a promising additive for preserving ovarian tissue integrity, at least for primordial follicles, up to 21 days post-transplantation.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea.|Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea.
authors.forename: Hee Jun
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea.|Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea.
authors.forename: Jaewang
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea.
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Youm
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea. leejrmd@snu.ac.kr.|Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea. leejrmd@snu.ac.kr.
authors.forename: Jung Ryeol
authors.initials: JR
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea.|Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea.
authors.forename: Chang Suk
authors.initials: CS
authors.lastname: Suh
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea.
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D008815:Mice, Inbred Strains; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology; D065851:Ovarian Reserve / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000637:transplantation*; D018384:Oxidative Stress / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D014182:Transplantation, Autologous; D058989:Vitrification
chemical_list: D016166:Free Radical Scavengers; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase
keywords: Fertility preservation; Ovarian tissue cryopreservation; Ovarian tissue transplantation; Polyethylene glycol-superoxide dismutase (PEG-SOD)