title: First proteome study of sporadic flowering in bamboo species (Bambusa vulgaris and Dendrocalamus manipureanus) reveal the boom is associated with stress and mobile genetic elements.
abstract: Bamboo species are the fastest-growing plants having a long vegetative cycle. Abrupt switching from the vegetative phase to the reproductive phase via sporadic flowering boom, occasionally leads to death of bamboo clumps, and threatens the existence of many bamboo species. To apprehend the molecular mechanism driving sporadic flowering, proteome changes in the initial and advanced floral buds of two edible bamboo species (Bambusa vulgaris and Dendrocalamus manipureanus) was dissected by two-dimensional gel electrophoresis (2-DE). A total of 39 differentially expressed peptide spots were identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF-TOF/MS). In both B. vulgaris and D. manipureanus, identified proteins were categorized as transposon-related, defence and stress-related, cell cycle related, metabolism related, signal transduction related, and some lacked known putative domains. Proteins such as SEPALLATA3, ubiquitin, histone 3, thaumatin-like protein, putative tethering factor, SF-assemblin, polyubiquitin, mitochondrial carrier-like protein and RPT2-like protein were significantly expressed. Differences in D. manipureanus and B. vulgaris suggested that bamboo species have diverse 'drivers' or 'passengers' genes that govern natural sporadic flowering boom. This first floral proteomics analysis of bamboos revealed that sporadic boom is a highly energetic process, associated with stress elements, mobile genetic elements and signal transduction cross-talk elements.
authors.affiliation: Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal 795001, Manipur, India; The University of Health and Allied Sciences, School of Basic and Biomedical Science, PMB 31 Ho, Volta Region, Ghana. Electronic address: bengyellalouis@gmail.com.
authors.forename: Bengyella
authors.initials: B
authors.lastname: Louis
authors.affiliation: Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal 795001, Manipur, India; The University of Health and Allied Sciences, School of Basic and Biomedical Science, PMB 31 Ho, Volta Region, Ghana. Electronic address: Sayanikawaikhom@gmail.com.
authors.forename: Sayanika Devi
authors.initials: SD
authors.lastname: Waikhom
authors.affiliation: Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal 795001, Manipur, India. Electronic address: saivir9@yahoo.com.
authors.forename: Sailendra
authors.initials: S
authors.lastname: Goyari
authors.affiliation: Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal 795001, Manipur, India. Electronic address: robinsoncjose@yahoo.co.in.
authors.forename: Robinson C
authors.initials: RC
authors.lastname: Jose
authors.affiliation: Department of Biotechnology, Haldia Institute of Technology, Haldia 721657, West Bengal, India. Electronic address: prpranabroy94@gmail.com.
authors.forename: Pranab
authors.initials: P
authors.lastname: Roy
authors.affiliation: Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal 795001, Manipur, India; Institute of Advanced Study in Science and Technology (IASST), Boragaon, Assam, India. Electronic address: nctalukdar@yahoo.com.
authors.forename: Narayan Chandra
authors.initials: NC
authors.lastname: Talukdar
mesh_terms: D031723:Bambusa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020071:Interspersed Repetitive Sequences / Q000502:physiology*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Bamboo spikelets; Inflorescence; Proteome; SEPALLATA3; Two dimensional gel electrophoresis; Ubiquitin