title: Titanium-35niobium alloy as a potential material for biomedical implants: In vitro study.
abstract: Research on new titanium alloys and different surface topographies aims to improve osseointegration. The objective of this study is to analyze the behavior of osteogenic cells cultivated on porous and dense samples of titanium-niobium alloys, and to compare them with the behavior of such type of cells on commercial pure titanium. Samples prepared using powder metallurgy were characterized using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and metallographic and profilometer analyses. Osteogenic cells from newborn rat calvaria were plated over different groups: dense or porous samples composed of Ti or Ti-35niobium (Nb). Cell adhesion, cell proliferation, MTT assay, cell morphology, protein total content, alkaline phosphatase activity, and mineralization nodules were assessed. Results from XRD and EDS analysis confirmed the presence of Ti and Nb in the test alloy. Metallographic analysis revealed interconnected pores, with pore size ranging from 138 to 150μm. The profilometer analysis detected the greatest rugosity within the dense alloy samples. In vitro tests revealed similar biocompatibility between Ti-35Nb and Ti; furthermore, it was possible to verify that the association of porous surface topography and the Ti-35Nb alloy positively influenced mineralized matrix formation. We propose that the Ti-35Nb alloy with porous topography constitutes a biocompatible material with great potential for use in biomedical implants.
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil.
authors.forename: Dennia Perez
authors.initials: DP
authors.lastname: de Andrade
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil.
authors.forename: Luana Marotta Reis
authors.initials: LM
authors.lastname: de Vasconcellos
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil.
authors.forename: Isabel Chaves Silva
authors.initials: IC
authors.lastname: Carvalho
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil.
authors.forename: Lilibeth Ferraz de Brito Penna
authors.initials: LF
authors.lastname: Forte
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil.
authors.forename: Evelyn Luzia
authors.initials: EL
authors.lastname: de Souza Santos
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil. Electronic address: renatafalchete@hotmail.com.
authors.forename: Renata Falchete do
authors.initials: RF
authors.lastname: Prado
authors.affiliation: Division of Materials, Air and Space Institute, CTA, Praça Mal. do Ar Eduardo Gomes, 14, São José dos Campos 12904-000, SP, Brazil.
authors.forename: Dalcy Roberto Dos
authors.initials: DR
authors.lastname: Santos
authors.affiliation: Division of Materials, Air and Space Institute, CTA, Praça Mal. do Ar Eduardo Gomes, 14, São José dos Campos 12904-000, SP, Brazil.
authors.forename: Carlos Alberto Alves
authors.initials: CA
authors.lastname: Cairo
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ Estadual Paulista, State University of São Paulo (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos 12245-000, SP, Brazil.
authors.forename: Yasmin Rodarte
authors.initials: YR
authors.lastname: Carvalho
mesh_terms: D000497:Alloys / Q000737:chemistry*; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion; D002478:Cells, Cultured; D008422:Materials Testing; D016348:Osseointegration; D010012:Osteogenesis; D016062:Porosity; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000497:Alloys; D018786:Bone Substitutes; C575293:titanium-niobium alloy
keywords: Biocompatibility; Implants; Osteogenesis; Porous surfaces; Titanium niobium alloy