title: Dysfunctional mitochondria regulate the size of root apical meristem and leaf development in Arabidopsis.
abstract: Mitochondria play an important role in maintaining metabolic and energy homeostasis in the plant cell. Thus, perturbation of mitochondrial structure and function will affect plant growth and development. Arabidopsis slow growth3 (slo3) is defective in At3g61360 that encodes a pentatricopeptide repeat (PPR) protein. Analysis of slo3 mitochondrial RNA metabolism revealed that the splicing of nad7 intron 2 is impaired, which leads to a dramatic reduction in complex I activity. So the SLO3 PPR protein is a splicing factor that is required for the removal of nad7 intron 2 in Arabidopsis. The slo3 mutant plants have obvious phenotypes with severe growth retardation and delayed development. The size of root apical meristem (RAM) is reduced and the production of meristem cells is decreased in slo3. Furthermore, the rosette leaves of slo3 are curled or crinkled, which may be derived from uneven growth of the leaf surface. The underlying mechanisms by which dysfunctional mitochondria affect these growth and developmental phenotypes have yet to be established. Nonetheless, plant hormone auxin is known to play an important role in orchestrating the development of RAM and leaf shape. It is possible that dysfunctional mitochondria may interact with auxin signaling pathways to regulate the boundary of RAM and the cell division arrest front during leaf growth in Arabidopsis.
authors.affiliation: a Institute of Plant and Microbial Biology; Academia Sinica ; Taipei , Taiwan.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Hsieh
authors.affiliation: a Institute of Plant and Microbial Biology; Academia Sinica ; Taipei , Taiwan.
authors.forename: Jo-Chien
authors.initials: JC
authors.lastname: Liao
authors.affiliation: a Institute of Plant and Microbial Biology; Academia Sinica ; Taipei , Taiwan.
authors.forename: Ming-Hsiun
authors.initials: MH
authors.lastname: Hsieh
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D059447:Cell Cycle Checkpoints; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D007438:Introns; D018519:Meristem / Q000254:growth & development / Q000378:metabolism; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012326:RNA Splicing; D000072260:RNA Splicing Factors; D018749:RNA, Plant / Q000378:metabolism; D036226:Seedlings; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D024101:Mitochondrial Proteins; D000072260:RNA Splicing Factors; D018749:RNA, Plant; C000605230:SLO3 protein, Arabidopsis; C560633:NAD7 protein, Arabidopsis; D009245:NADH Dehydrogenase
keywords: NADH dehydrogenase; auxin; curly leaf; mitochondria; root apical meristem