title: Alkaloid variation in New Zealand kōwhai, Sophora species.
abstract: Alkaloid contents of leaf and seed samples of eight species of Sophora native to New Zealand, plus Sophora cassioides from Chile are reported. Fifty-six leaf and forty-two seed samples were analysed for alkaloid content by proton nuclear magnetic resonance spectroscopy, which showed major alkaloids as cytisine, N-methyl cytisine and matrine. GC analyses quantified these and identified further alkaloid components. The alkaloids identified were cytisine, sparteine, and matrine-types common to Sophora from other regions of the world. Cytisine, N-methyl cytisine, and matrine were generally the most abundant alkaloids across all species with seeds containing the highest concentrations of alkaloids. However, there was no clear taxonomic grouping based on alkaloid composition. A quantitative analysis of various parts of two Sophora microphylla trees showed that the seeds were the richest source of alkaloids (total 0.4-0.5% DM), followed by leaf and twig (0.1-0.3%) and then bark (0.04-0.06%), with only low amounts (<0.02%) found in the roots. This study represents the most comprehensive phytochemical investigation of New Zealand Sophora species to date and presents data for three species of Sophora for which no prior chemistry has been reported.
authors.affiliation: Department of Chemistry and Biochemistry, Boise State University, 1910 University Drive, Boise, ID 83725-1520, USA.
authors.forename: Owen M
authors.initials: OM
authors.lastname: McDougal
authors.affiliation: Allan Herbarium, Landcare Research, P.O. Box 69, Lincoln, New Zealand.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Heenan
authors.affiliation: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 4704, Christchurch 8140, New Zealand.
authors.forename: Peter
authors.initials: P
authors.lastname: Jaksons
authors.affiliation: Plant Extracts Research Unit, The New Zealand Institute for Plant & Food Research Ltd, Department of Chemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Sansom
authors.affiliation: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 4704, Christchurch 8140, New Zealand.
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Smallfield
authors.affiliation: Plant Extracts Research Unit, The New Zealand Institute for Plant & Food Research Ltd, Department of Chemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand.
authors.forename: Nigel B
authors.initials: NB
authors.lastname: Perry
authors.affiliation: Plant Extracts Research Unit, The New Zealand Institute for Plant & Food Research Ltd, Department of Chemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand. Electronic address: john.vanklink@plantandfood.co.nz.
authors.forename: John W
authors.initials: JW
authors.lastname: van Klink
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001392:Azocines / Q000032:analysis; D002677:Chile; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D015394:Molecular Structure; D009520:New Zealand; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011807:Quinolizines / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D029909:Sophora / Q000737:chemistry* / Q000235:genetics; D000093842:Matrines; D000093843:Quinolizidine Alkaloids
chemical_list: D000470:Alkaloids; D001392:Azocines; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D000093842:Matrines; C004712:cytisine; D000093843:Quinolizidine Alkaloids; C034245:N-methylcytisine
keywords: Fabaceae; GC–MS; Sophora chathamica; Sophora fulvida; Sophora godleyi; Sophora longicarinata; Sophora microphylla; Sophora molloyi; Sophora prostrata; Sophora tetraptera