title: Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology.
abstract: The multigram-scale synthesis of a sulfation-site programmed heparin-like dodecasaccharide is described. Evaluation alongside dodecasaccharides lacking this single glucosamine O6-sulfation, or having per-O6-sulfation, shows that site-specific modification of the terminal glucosamine dramatically interconverts regulation of in vitro and in vivo biology mediated by the two important chemokines, CXCL12 (SDF1α) or CXCL8 (IL-8).
authors.affiliation: Christie Hospital, Institute of Cancer Studies, University of Manchester, Manchester, M20 4BX, UK.
authors.forename: Gordon C
authors.initials: GC
authors.lastname: Jayson
authors.forename: Steen U
authors.initials: SU
authors.lastname: Hansen
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Miller
authors.forename: Claire L
authors.initials: CL
authors.lastname: Cole
authors.forename: Graham
authors.initials: G
authors.lastname: Rushton
authors.forename: Egle
authors.initials: E
authors.lastname: Avizienyte
authors.forename: John M
authors.initials: JM
authors.lastname: Gardiner
mesh_terms: D002240:Carbohydrate Sequence; D054441:Cell Migration Assays, Leukocyte; D054377:Chemokine CXCL12 / Q000037:antagonists & inhibitors*; D006497:Heparan Sulfate / Q000737:chemistry* / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D007962:Leukocytes; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D054377:Chemokine CXCL12; D006497:Heparan Sulfate; D016209:Interleukin-8; D009844:Oligosaccharides; C515255:CXCL12 protein, human; C508606:CXCL8 protein, human