title: Pre-staining of glycoprotein in SDS-PAGE by the synthesis of a new hydrazide derivative.
abstract: In this study, a new hydrazide derivative (UGF202) was synthesized and introduced as a highly sensitive and selective fluorescent probe to pre-stain glycoproteins in 1D and 2D SDS-PAGE. As low as 0.5-1 ng glycoproteins (transferrin, α1-acid glycoprotein, avidin) could be selectively detected, which is comparable to that of Pro-Q Emerald 300 stain, one of the most sensitive and commonly used glycoprotein staining kit. In addition, the specificity of the newly developed method was confirmed by the study of de-glycosylation, glycoproteins affinity enrichment and LC-MS/MS, respectively. According to the results, it is concluded that UGF202 pre-stain can provide an alternative for the visualization of gel-separated glycoproteins.
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Ayi
authors.initials: A
authors.lastname: Zhou
authors.affiliation: Clinical Laboratory Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Tieli
authors.initials: T
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Yu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Shen
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.|Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.|Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.
authors.forename: Litai
authors.initials: L
authors.lastname: Jin
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of neuroscience, Department of histology and embryology, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001360:Avidin / Q000032:analysis; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D009961:Orosomucoid / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods; D053719:Tandem Mass Spectrometry; D014168:Transferrin / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D006023:Glycoproteins; D006834:Hydrazines; D009961:Orosomucoid; D014168:Transferrin; D001360:Avidin
keywords: Electrophoresis; Glycoprotein detection; Glycoproteomics; Pre-stain; Pro-Q Emerald 300; UGF202