title: Asymmetric AB3 Miktoarm Star Polymers: Synthesis, Self-Assembly, and Study of Micelle Stability Using AF4 for Efficient Drug Delivery.
abstract: A simple and versatile methodology, which employs a combination of ring-opening polymerization and alkyne-azide click chemistry to synthesize amphiphilic AB3 miktoarm stars, is reported. Their aqueous self-assembly behavior was studied using dynamic light scattering, fluorescence, and asymmetrical flow field-flow fractionation (AF4). AB3 miktoarm stars form micelles which incorporate curcumin with high efficiency, and significantly reduce the viability of glioblastoma cells in spheroids. We demonstrate that AF4 is an effective technique to determine the size distribution of self-assembled structures exposed to a biological medium. The ease, with which asymmetric AB3 miktoarm polymers are constructed, provides a platform that can be widely employed to deliver a variety of lipophilic drugs.
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Moquin
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Anjali
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Anthony
authors.initials: A
authors.lastname: Lau
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada. dusica.maysinger@mcgill.ca.
authors.forename: Dusica
authors.initials: D
authors.lastname: Maysinger
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montreal, Quebec H3A 0B8, Canada. ashok.kakkar@mcgill.ca.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kakkar
mesh_terms: D063566:Biodegradable Plastics / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D057930:Click Chemistry; D003474:Curcumin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems; D005909:Glioblastoma / Q000188:drug therapy* / Q000378:metabolism*; D006801:Humans; D008823:Micelles
chemical_list: D063566:Biodegradable Plastics; D008823:Micelles; D003474:Curcumin
keywords: amphiphilic miktoarm star polymer; drug delivery systems; field-flow fractionation; glioblastoma spheroids; micelles