title: Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures.
abstract: Tuberculosis (TB) in goats (Capra hircus) is due to infection with members of the Mycobacterium tuberculosis complex (MTC), mainly Mycobacterium bovis and Mycobacterium caprae. We report a comparative experimental infection of goats with M. bovis, M. caprae and M. tuberculosis strains. We hypothesized that goats experimentally infected with different members of the MTC would display different clinical pictures. Three groups of goats were challenged with either M. bovis SB0134 (group 1, n=5), M. caprae SB0157 (group 2, n=5) and M. tuberculosis SIT58 (group 3, n=4). The highest mean total lesion score was observed in M. bovis challenged goats (mean 15.2, range 9-19), followed by those challenged with M. caprae (10.8, 2-23). The lowest score was recorded in goats challenged with M. tuberculosis (3, 1-6). Culture results coincided with the lesion scores in yielding more positive pools (7/15) in M. bovis challenged goats. By contrast, only three pools were positive from goats challenged M. tuberculosis (3/12) and with M. caprae (3/15), respectively. Differences in the performance of the intradermal and gamma-interferon (IFN-γ) tests depending of the group were observed since all goats from group 1 were diagnosed using intradermal test and these goats reacted earlier to the IFN-γ assay in comparison to the other groups. This study confirmed that goats experimentally infected with different members of the MTC display different clinical pictures and this fact may have implications for MTC maintenance and bacterial shedding.
authors.affiliation: MAEVA SERVET S.L., Alameda del Valle, Madrid, Spain; Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, Madrid, Spain. Electronic address: jbezosga@visavet.ucm.es.
authors.forename: J
authors.initials: J
authors.lastname: Bezos
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Casal
authors.affiliation: Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, United States of America.
authors.forename: I
authors.initials: I
authors.lastname: Díez-Delgado
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Romero
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Liandris
authors.affiliation: Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, United States of America.
authors.forename: J
authors.initials: J
authors.lastname: Álvarez
authors.affiliation: Departamento de Sanidad Animal, Neiker-Tecnalia, Derio, Bizkaia, Spain.
authors.forename: I A
authors.initials: IA
authors.lastname: Sevilla
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: L de
authors.initials: Ld
authors.lastname: Juan
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Domínguez
authors.affiliation: SaBio IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Gortázar
mesh_terms: D000818:Animals; D015511:Goat Diseases / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology*; D006041:Goats; D007371:Interferon-gamma / Q000097:blood; D009161:Mycobacterium / Q000276:immunology / Q000472:pathogenicity; D009163:Mycobacterium bovis / Q000276:immunology* / Q000472:pathogenicity*; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000472:pathogenicity*; D013030:Spain; D013045:Species Specificity; D014374:Tuberculin Test / Q000379:methods / Q000662:veterinary; D014376:Tuberculosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D007371:Interferon-gamma
keywords: Animal tuberculosis; Domestic goat; Lesion score; Shedding; Strain genotype