title: Self-degrading niosomes for encapsulation of hydrophilic and hydrophobic drugs: An efficient carrier for cancer multi-drug delivery.
abstract: In this study, we have examined the encapsulation and release of hydrophilic and hydrophobic drugs in self-degrading niosomes as a unique method for anticancer therapy. Niosomes were prepared by amphiphilic self-assembly of Tween 80 and cholesterol through film hydration method. Encapsulation studies with two active molecules curcumin and doxorubicin hydrochloride (Dox) showed that curcumin is supposed to accumulate in the shell whereas Dox accumulates in the inner aqueous core of the niosome. Confocal studies indicated that nile red adsorbs preferentially to the head group of the Tween 80 and forms two separate layers in the shell. It was also seen that the niosomes undergo self-degradation in PBS through a sequential process, forming interconnected pores followed by complete collapse after 1week. The release profile shows two phases: i) initial Dox release in the first two days, followed by ii) curcumin release over 7days. Enhanced (synergistic) cytotoxicity was observed for dual-drug loaded niosomes against HeLa cell lines. Thus these niosomes are shown to offer a promising delivery system for hydrophobic and hydrophilic drugs collectively.
authors.affiliation: Department of Biomedical Engineering, SRM University, Kattankulathur, Chennai 603203, India.
authors.forename: Varsha
authors.initials: V
authors.lastname: Sharma
authors.affiliation: SRM Research Institute, SRM University, Kattankulathur, Chennai 603203, India. Electronic address: rsanandhakumar@gmail.com.
authors.forename: Sundaramurthy
authors.initials: S
authors.lastname: Anandhakumar
authors.affiliation: SRM Research Institute, SRM University, Kattankulathur, Chennai 603203, India.
authors.forename: Manickam
authors.initials: M
authors.lastname: Sasidharan
mesh_terms: D002784:Cholesterol / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003474:Curcumin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy* / Q000648:ultrastructure; D011136:Polysorbates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D008081:Liposomes; D011136:Polysorbates; D004317:Doxorubicin; D002784:Cholesterol; D003474:Curcumin
keywords: Cancer; Multi-drug delivery and hydrophobic drugs; Niosomes; Self-degradation