title: (E)-β-farnesene gene reduces Lipaphis erysimi colonization in transgenic Brassica juncea lines.
abstract: Aphids are the major concern that significantly reduces the yield of crops. (E)-β-farnesene (Eβf) is the principal component of the alarm pheromone of many aphids. The results of current research support the direct defense response of (E)-β-farnesene (Eβf) against aphid Lipaphis erysimi (L.) Kaltenbach in Brassica juncea. Eβf gene was isolated from Mentha arvensis and transformed into B. juncea, showed direct repellent against aphid colonization. The seasonal mean population (SMP) recorded under field condition showed significantly higher aphid colonization in wild type in comparison to most of the transgenic lines, and shows positive correlation with the repellency of transgenic plant expressing (E)-β-farnesene. The current research investigation provides direct evidence for aphid control in B. juncea using Eβf, a non-toxic mode of action.
authors.affiliation: a Agricultural and Agri-Food Canada ; Lethbridge , Canada.
authors.forename: Shiv Shankar
authors.initials: SS
authors.lastname: Verma
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sinha
authors.forename: Anajna
authors.initials: A
authors.lastname: Jajoo
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D017343:Genes, Plant; D009149:Mustard Plant / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology*; D018515:Plant Leaves / Q000502:physiology; D030821:Plants, Genetically Modified; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012717:Sesquiterpenes; C036483:T-DNA; C062671:beta-farnesene
keywords: (E)-β-farnesene; Brassica juncea; CTAB, cetyltrimethyl ammonium bromide; Eβf, (E)-β-farnesene; Lipaphis erysimi; PCR, polymerase chain reaction; RT-PCR, real time polymerase chain reaction; WT, wild-type; sesquiterpene; transgenic plant