title: Evaluation of the updated RIDA®QUICK (Version N1402) immunochromatographic assay for the detection of norovirus in clinical specimens.
abstract: The sensitivity and specificity of the R-Biopharm RIDA(®)QUICK (N1402) immunochromatography assay for norovirus detection was examined using fecal material from Australian gastroenteritis incidents. The study involved the analysis of 3 groups of specimens; group 1 comprised 100 norovirus open reading frame (ORF) 1 RT-PCR positive specimens; group 2 comprised 100 ORF 1 RT-PCR norovirus negative specimens and group 3 comprised 12 specimens containing common gastroenteritis viruses other than norovirus. The RIDA(®)QUICK (N1402) assay detected both GI and GII norovirus and had an overall sensitivity of 87%. Genotype analysis of the capsid region of the genome (ORF 2) indicated the RIDA(®)QUICK (N1402) assay could detect a range of genotypes including GI.1, GI.2, GI.3, GI.4, GI.5, GII.3, GII.4 (including variants GII.4 (2009-like), GII.4 (2012), GII.4 (2012-like) and GII.4 (unknown)), GII.6, GII.13 and GII.21. The assay had good sensitivity for both GI and GII norovirus. The assay had a specificity of 97% and did not cross react with a number of common fecal viruses. However, one of eight rotavirus positive, norovirus negative specimens gave a positive result; rotavirus cannot be taken as the cause of such a false positive but cannot be excluded either. The kit was quick and easy to use and would be valuable in point-of-care testing.
authors.affiliation: Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
authors.forename: Leesa D
authors.initials: LD
authors.lastname: Bruggink
authors.affiliation: Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
authors.forename: Natalie L
authors.initials: NL
authors.lastname: Dunbar
authors.affiliation: Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute, 792 Elizabeth St, Melbourne, Victoria 3000, Australia. Electronic address: john.marshall@mh.org.au.
authors.forename: John A
authors.initials: JA
authors.lastname: Marshall
mesh_terms: D001315:Australia; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D002846:Chromatography, Affinity / Q000379:methods*; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D005838:Genotype; D006801:Humans; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D013995:Time
keywords: Genotypes; Immunochromatography; Norovirus; Point-of-care testing; Sensitivity; Specificity