title: Molecular cloning of feline resistin and the expression of resistin, leptin and adiponectin in the adipose tissue of normal and obese cats.
abstract: Resistin, one of the adipokines that has a cycteine-rich C-terminus, is considered to relate to the development of insulin resistance in rats. However, in cats, there is little knowledge regarding resistin. In this study, we cloned the feline resistin cDNA from adipose tissue by RT-PCR. The feline resistin clone contained an entire open reading frame encoding 107 amino acids that had 72.8%, 75.4%, 50.9% and 51.8% homology with bovine, human, mouse and rat homologues, respectively. In both subcutaneous and visceral adipose tissues, the transcription levels of feline resistin mRNA were significantly higher in obese cats than normal cats, and those of feline adiponectin mRNA were significantly lower in obese cats than normal cats. However, there was no difference in the expression of feline leptin between normal and obese cats. On the other hand, in both normal and obese cats, there were no significant differences in resistin, leptin and adiponectin mRNA levels between subcutaneous and visceral adipose tissues. In cats, the altered expression of resistin and adiponectin mRNA with obesity may contribute to the pathogenesis of insulin resistance and subsequent diabetes mellitus. In addition to feline adiponectin, the feline resistin cDNA clone obtained in this study will be useful for further investigation of the pathogenesis of obesity in cats.
authors.affiliation: Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takashima
authors.forename: Naohito
authors.initials: N
authors.lastname: Nishii
authors.forename: Akiko
authors.initials: A
authors.lastname: Kato
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Matsubara
authors.forename: Sanae
authors.initials: S
authors.lastname: Shibata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kitagawa
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002371:Cat Diseases / Q000235:genetics / Q000378:metabolism*; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D008297:Male; D051379:Mice; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D051381:Rats; D052243:Resistin / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D052242:Adiponectin; D020738:Leptin; D052243:Resistin