title: Ultrasensitive Electrochemical Biosensor for HIV Gene Detection Based on Graphene Stabilized Gold Nanoclusters with Exonuclease Amplification.
abstract: Because human immunodeficiency virus (HIV) has been one of the most terrible viruses in recent decades, early diagnosis of the HIV gene is of great importance for all scientists around the world. In our work, we developed a novel electrochemical biosensor based on one-step ultrasonic synthesized graphene stabilized gold nanocluster (GR/AuNC) modified glassy carbon electrode (GCE) with an exonuclease III (Exo III)-assisted target recycling amplification strategy for the detection of HIV DNA. It is the first time that GR/AuNCs have been used as biosensor platform and aptamer with cytosine-rich base set as capture probe to construct the biosensor. With the combination of cytosine-rich capture probe, good conductivity and high surfaces of GR/AuNCs, and Exo III-assisted target recycling amplification, we realized high sensitivity and good selectivity detection of target HIV DNA with a detection limit of 30 aM (S/N = 3). Furthermore, the proposed biosensor has a promising potential application for target detection in human serum analysis.
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, and College of Chemistry and Chemical Engineering, Hubei University , Wuhan 430062, People's Republic of China.
authors.forename: Yijia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, and College of Chemistry and Chemical Engineering, Hubei University , Wuhan 430062, People's Republic of China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Bai
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, and College of Chemistry and Chemical Engineering, Hubei University , Wuhan 430062, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wen
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, and College of Chemistry and Chemical Engineering, Hubei University , Wuhan 430062, People's Republic of China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, and College of Chemistry and Chemical Engineering, Hubei University , Wuhan 430062, People's Republic of China.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D000067493:Dynamic Light Scattering; D055664:Electrochemical Techniques; D004566:Electrodes; D005090:Exodeoxyribonucleases / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006678:HIV / Q000235:genetics*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral; D006046:Gold; D006108:Graphite; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III
keywords: HIV gene; cytosine-rich capture probe; electrochemistry detection; exonuclease III-assisted target recycling; graphene stabilized gold nanoclusters