title: Low-Transition-Temperature Mixtures (LTTMs) for Dissolving Proteins and for Drug Formulation.
abstract: Several diverse proteins are found to readily dissolve in neat low-transition-temperature mixtures (LTTMs). They undergo no irreversible denaturation in such unusual solvents, and the resistance of hen egg-white lysozyme against thermoinactivation in LTTMs is greater than in aqueous solution at extreme pHs. Separately, the water-sensitive drug aspirin is found to form concentrated transparent LTTMs, where it is some 10-fold more stable against cleavage than in water.
authors.affiliation: Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. ezhsu@njfu.edu.cn.|Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China. ezhsu@njfu.edu.cn.
authors.forename: Erzheng
authors.initials: E
authors.lastname: Su
authors.affiliation: Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. klibanov@mit.edu.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Klibanov
mesh_terms: D000818:Animals; D001241:Aspirin / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D044366:Transition Temperature; D014867:Water / Q000737:chemistry
chemical_list: D011506:Proteins; D012997:Solvents; D014867:Water; D001241:Aspirin
keywords: Drug formulation; Low-transition-temperature mixtures; Protein solubility; Water-sensitive drugs