title: Thiol/disulfide homeostasis in asphalt workers.
abstract: The aim of this study was to investigate thiol/disulfide homeostasis in asphalt workers who are exposed to polycyclic aromatic hydrocarbons occupationally. The study was carried out in 34 nonsmoker asphalt workers. Additionally, 35 healthy nonsmoker volunteers were recruited as control group. Thiol and disulfide concentrations were determined using the novel automated measurement method. Levels of urinary 1-OH-pyrene were analyzed by liquid chromatography. Disulfide/thiol ratio was significantly higher in exposed group (p = .034). Also, a positive correlation was detected between disulfide/thiol ratio and 1-OH-pyrene values (r = .249, p = .036). Thiol/disulfide homeostasis was found to be disturbed in asphalt workers. The novel test used in this study may be useful for evaluating the oxidative status in polycyclic aromatic hydrocarbon (PAH) exposure.
authors.affiliation: a Department of Public Health , Yıldırım Beyazıt University , Ankara , Turkey.
authors.forename: Ömer Hınç
authors.initials: ÖH
authors.lastname: Yilmaz
authors.affiliation: b Department of Biochemistry , Occupational Diseases Hospital , Ankara , Turkey.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Bal
authors.affiliation: c Department of Biochemistry , Atatürk Educational and Research Hospital , Ankara , Turkey.
authors.forename: Salim
authors.initials: S
authors.lastname: Neşelioglu
authors.affiliation: d Department of Pharmacology , Occupational Diseases Hospital , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Büyükşekerci
authors.affiliation: e Department of Family Medicine , Occupational Diseases Hospital , Ankara , Turkey.
authors.forename: Meşide
authors.initials: M
authors.lastname: Gündüzöz
authors.affiliation: c Department of Biochemistry , Atatürk Educational and Research Hospital , Ankara , Turkey.
authors.forename: Funda
authors.initials: F
authors.lastname: Eren
authors.affiliation: f Department of Chemical Engineering and Bioengineering Division , Hacettepe University , Ankara , Turkey.
authors.forename: Lutfiye
authors.initials: L
authors.lastname: Tutkun
authors.affiliation: g Department of Biochemistry , Yıldırım Beyazıt University Medical Faculty , Ankara , Turkey.
authors.forename: Fatma Meric
authors.initials: FM
authors.lastname: Yilmaz
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000097:blood*; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D060389:Construction Industry; D004220:Disulfides / Q000097:blood*; D006706:Homeostasis; D006801:Humans; D006838:Hydrocarbons; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D018384:Oxidative Stress; D011721:Pyrenes / Q000652:urine; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000097:blood*; D014421:Turkey
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D004220:Disulfides; D006838:Hydrocarbons; D011721:Pyrenes; D013438:Sulfhydryl Compounds; C006647:asphalt; C033146:1-hydroxypyrene
keywords: Asphalt workers; oxidative stress; polycyclic aromatic hydrocarbons; thiol/disulfide ratio