title: BRAF activated non-coding RNA (BANCR) promoting gastric cancer cells proliferation via regulation of NF-κB1.
abstract: OBJECTIVE
  Long non-coding RNA, BANCR, has been demonstrated to contribute to the proliferation and migration of tumors. However, its molecular mechanism underlying gastric cancer is still unknown. In present study, we investigated whether BANCR was involved in the development of gastric cancer cells via regulation of NF-κB1.
  
  
  METHODS
  Human gastric cancer tissues were isolated as well as human gastric cell lines MGC803 and BGC823 were cultured to investigate the role of BANCR in gastric cancer.
  
  
  RESULTS
  BANCR expression was significantly up-regulated in gastric tumor tissues and gastric cell lines. Down-regulation of BANCR inhibited gastric cancer cell growth and promoted cell apoptosis, and it also contributed to a significant decrease of NF-κB1 (P50/105) expression and 3'UTR of NF-κB1 activity. Overexpression of NF-κB1 reversed the effect of BANCR on cancer cell growth and apoptosis. MiroRNA-9 (miR-9) targeted NF-κB1, and miR-9 inhibitor also reversed the effects of BANCR on gastric cancer cell growth and apoptosis.
  
  
  CONCLUSIONS
  BANCR was highly expressed both in gastric tumor tissues and in cancer cells. NF-κB1 and miR-9 were involved in the role of BANCR in gastric cancer cell growth and apoptosis.
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China.
authors.forename: Xin-Yang
authors.initials: XY
authors.lastname: Lu
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Ning
authors.affiliation: Department of Pathology, Affiliated Hospital of Jining Medical University, Jining 272029, China.
authors.forename: Chuan-Tao
authors.initials: CT
authors.lastname: Yuan
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining 272029, China. Electronic address: wang_ailiang@126.com.
authors.forename: Ai-Liang
authors.initials: AL
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D009368:Neoplasm Transplantation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf
keywords: BANCR; Gastric cancer; Long non-coding RNA; NF-κB; miR-9