title: The effect of kisspeptin on the regulation of vascular tone.
abstract: Kisspeptin has been implicated in cardiovascular control. Eicosanoids play a crucial role in the activation of platelets and the regulation of vascular tone. In the present study, we investigated the effect of kisspeptins on eicosanoid synthesis in platelets and aorta in vitro. Platelets and aorta were isolated from Wistar-Kyoto rats. After preincubation with different doses of kisspeptin, samples were incubated with [1-(14)C]arachidonic acid (0.172 pmol/mL) in tissue culture Medium 199. The amount of labeled eicosanoids was measured with liquid scintillation, after separation with overpressure thin-layer chromatography. Kisspeptin-13 stimulated the thromboxane synthesis. The dose-response curve was bell-shaped and the most effective concentration was 2.5 × 10(-8) mol/L, inducing a 27% increase. Lipoxygenase products of platelets displayed a dose-dependent elevation up to the dose of 5 × 10(-8) mol/L. In the aorta, kisspeptin-13 induced a marked elevation in the production of 6-keto-prostaglandin F1α, the stable metabolite of prostacyclin, and lipoxygenase products. Different effects of kisspeptin on cyclooxygenase and lipoxygenase products indicate that beyond intracellular Ca(2+) mobilization, other signaling pathways might also contribute to its actions. Our data suggest that kisspeptin, through the alteration of eicosanoid synthesis in platelets and aorta, may play a physiologic and (or) pathologic role in the regulation of vascular tone.
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Mezei
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Omid
authors.initials: O
authors.lastname: Zamani-Forooshani
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Csabafi
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Bence
authors.initials: B
authors.lastname: Szikszai
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Papp
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Ónodi
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Dóra
authors.initials: D
authors.lastname: Török
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Leprán
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Gyula
authors.initials: G
authors.lastname: Telegdy
authors.affiliation: Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.|Department of Pathophysiology, University of Szeged, P.O. Box 427, H-6701, Semmelweis u. 1, 6725 Szeged, Hungary.
authors.forename: Gyula
authors.initials: G
authors.lastname: Szabó
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015777:Eicosanoids / Q000096:biosynthesis*; D059648:Kisspeptins / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013931:Thromboxanes / Q000096:biosynthesis; D014661:Vasoconstriction / Q000502:physiology*
chemical_list: D015777:Eicosanoids; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D013931:Thromboxanes; D016718:Arachidonic Acid; D015121:6-Ketoprostaglandin F1 alpha
keywords: aorta; aorte; eicosanoids; eicosanoïdes; kisspeptin; kisspeptine; plaquettes; platelets