title: Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids.
abstract: The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Escherichia coli and Staphylococcus aureus. The effect of alkaloids on bacteria growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor (TAM) air isothermal microcalorimeter and parameters such as growth rate constant (μ), peak-time (Tm), inhibitory ratio (I), and enhancement ratio (E) were calculated. The relationships between the concentration of Aconitum alkaloids and μ of E. coli or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coli and had a potentially inhibitory effect on the growth of S. aureus.
authors.affiliation: School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan 250353, China.
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Shi
authors.affiliation: Beijing Health Vocational College, Beijing 100053, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.affiliation: School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.affiliation: School of Pharmaceutical Science, Shandong University, Jinan 250012, China; guimeilin@sdu.edu.cn.
authors.forename: Gui-mei
authors.initials: GM
authors.lastname: Lin
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000008:administration & dosage*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002151:Calorimetry / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000008:administration & dosage*
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: Aconitum alkaloids; Escherichia coli; Microcalorimetry; Staphylococcus aureus