title: Eight new cycloartane triterpenoids from Beesia calthifolia with hepatoprotective effects against D-galactosamine induced L02 cell damage.
abstract: Fourteen 20,24-epoxy-cycloartane triterpenoids, including eight new ones (1-8), were isolated from 95% ethanol extract of the rhizomes of Beesia calthifolia. Their structures were determined by spectroscopic and chemical methods, especially 2D NMR and HRMS techniques. Among them, four new compounds (1-4) possess carbonyl groups at C-16, which were rarely found in cycloartane triterpenoids from this genus. Relative configuration at C-12 in beesioside III (9) and its aglycone (10) was revised to be 12α-OH rather than the reported 12β-OH. Some of the compounds showed potential hepatoprotective activities against human hepatic L02 cell damage induced by d-galactosamine.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li-She
authors.initials: LS
authors.lastname: Gan
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Dan-Jun
authors.initials: DJ
authors.lastname: Zheng
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Min-Zhe
authors.initials: MZ
authors.lastname: Zhang
authors.affiliation: Zhejiang Chemical Industry Research Institute Co., Ltd, Hangzhou 310023, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Bai-Hao
authors.initials: BH
authors.lastname: Shao
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jian-Xia
authors.initials: JX
authors.lastname: Mo
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: zhoucx10@zju.edu.cn.
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Zhou
mesh_terms: D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005688:Galactosamine / Q000633:toxicity*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D015394:Molecular Structure; D029626:Ranunculaceae / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D014315:Triterpenes; C085352:cycloartane; D005688:Galactosamine
keywords: Beesia calthifolia; Configuration; Cycloartane triterpenoids; Hepatoprotective activity; Structure elucidation