title: Intravenous immunoglobulin replacement induces an in vivo reduction of inflammatory monocytes and retains the monocyte ability to respond to bacterial stimulation in patients with common variable immunodeficiencies.
abstract: Intravenous IgG administration induces significant modifications in the innate and adaptive compartment of the immune system including the monocyte/macrophage system. We analyzed the in vivo effect of IgG administered at replacement dosages on the frequency of monocytes subsets, on the modulation of CD11b and sialic acid-binding immunoglobulin-like lectin receptor (Siglec 9) expression and on monocytes production of reactive oxygen species. We showed that patients with Common Variable Immune Deficiency have an increased frequency pro-inflammatory intermediate CD14(++)CD16(+) monocytes and an increased expression of CD11b and Siglec 9 on monocytes. IgG administered at replacement dosages exerted an in vivo anti-inflammatory effect as shown by a reduction of circulating monocytes, of intermediate pro-inflammatory monocytes, of CD11b and Siglec 9 expression and of ex vivo monocytes oxidative burst. Nevertheless, intravenous IgG administration did not affect the monocyte functional ability to respond to a bacterial stimulation in terms of CD11b and Siglec 9 expression and reactive oxygen species production.
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, Italy.
authors.forename: Filomena Monica
authors.initials: FM
authors.lastname: Cavaliere
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Prezzo
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Conti
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Bilotta
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Pulvirenti
authors.affiliation: Department of Paediatrics, Sapienza University of Rome, Italy.
authors.forename: Metello
authors.initials: M
authors.lastname: Iacobini
authors.affiliation: Department of Molecular Medicine, Sapienza University of Rome, Italy. Electronic address: isabella.quinti@uniroma1.it.
authors.forename: Isabella
authors.initials: I
authors.lastname: Quinti
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D039481:CD11b Antigen / Q000276:immunology; D017074:Common Variable Immunodeficiency / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D063265:Sialic Acid Binding Immunoglobulin-like Lectins / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; C509295:ITGAM protein, human; D016756:Immunoglobulins, Intravenous; C412227:SIGLEC9 protein, human; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D014409:Tumor Necrosis Factor-alpha
keywords: CD11b; CVID; IVIg; Monocytes; Oxidative burst; Siglec 9