title: Fluxes in PHA-storing microbial communities during enrichment and biopolymer accumulation processes.
abstract: The use of mixed microbial cultures for the production of polyhydroxyalkanoates (PHAs) is emerging as a viable technology. In this study, 16S rRNA gene amplicon pyrosequencing was used to analyse fluctuations in populations over a 63-day period within a PHA-storing mixed microbial community enriched on fermented whey permeate. This community was dominated by the genera Flavisolibacter and Zoogloea as well as an unidentified organism belonging to the phylum Bacteroidetes. The population was observed to cycle through an increase in Zoogloea followed by a return to a community composition similar to the initial one (highly enriched in Flavisolibacter). It was found that the PHA accumulation capacity of the community was robust to population flux during enrichment and even PHA accumulation, with final polymer composition dependent on the overall proportion of acetic to propionic acids in the feed. This community adaptation suggests that mixed culture PHA production is a robust process.
authors.affiliation: School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Om Murugan
authors.initials: OM
authors.lastname: Janarthanan
authors.affiliation: School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Bronwyn
authors.initials: B
authors.lastname: Laycock
authors.affiliation: School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Liliana
authors.initials: L
authors.lastname: Montano-Herrera
authors.affiliation: Advanced Water Management Centre, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Veolia Water Technologies AB - AnoxKaldnes, Klosterängsvägen 11A, SE-226 47 Lund, Sweden.
authors.forename: Monica V
authors.initials: MV
authors.lastname: Arcos-Hernandez
authors.affiliation: School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia; Veolia Water Technologies AB - AnoxKaldnes, Klosterängsvägen 11A, SE-226 47 Lund, Sweden. Electronic address: alan.werker@anoxkaldnes.com.
authors.forename: Alan
authors.initials: A
authors.lastname: Werker
authors.affiliation: School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia. Electronic address: s.pratt@uq.edu.au.
authors.forename: Steven
authors.initials: S
authors.lastname: Pratt
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D001704:Biopolymers / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D016013:Likelihood Functions; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001704:Biopolymers; D054813:Polyhydroxyalkanoates; D012336:RNA, Ribosomal, 16S