title: A Nicotinamide Adenine Dinucleotide Dispersed Multi-walled Carbon Nanotubes Electrode for Direct and Selective Electrochemical Detection of Uric Acid.
abstract: A nanocomposite platform built with multi-walled carbon nanotubes (MWCNTs) and nicotinamide adenine dinucleotide (NAD(+)) via a noncovalent interaction between the large π systems in NAD(+) molecules and MWCNTs on a glassy carbon substrate was successfully developed for the sensitive and selective detection of uric acid (UA) in the presence of ascorbic acid (AA), dopamine (DA). NAD(+) has an adenine subunit and a nicotinamide subunit, which enabled interaction with the purine subunit of UA through a strong π-π interaction to enhance the specificity of UA. Compared with a bare glassy carbon electrode (GCE) and MWCNTs/GCE, the MWCNTs-NAD(+)/GCE showed a low background current and a remarkable enhancement of the oxidation peak current of UA. Using differential pulse voltammetry (DPV), a high sensitivity for the determination of UA was explored for the MWCNTs-NAD(+) modified electrode. A linear relationship between the DPV peak current of UA and its concentration could be obtained in the range of 0.05 - 10 μM with the detection limit as low as 10 nM (S/N = 3). This present strategy provides a novel and promising platform for the detection of UA in human urine and serum samples.
authors.affiliation: Biology Institute of Shandong Academy of Sciences, Key Laboratory for Biosensors of Shandong Province.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Li
authors.forename: Yaohong
authors.initials: Y
authors.lastname: Ma
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Meng
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Jianguo
authors.initials: J
authors.lastname: Shi
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D057230:Limit of Detection; D009243:NAD / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D013997:Time Factors; D014527:Uric Acid / Q000032:analysis* / Q000097:blood / Q000737:chemistry* / Q000652:urine
chemical_list: D037742:Nanotubes, Carbon; D009243:NAD; D014527:Uric Acid; D001205:Ascorbic Acid; D004298:Dopamine