title: Immobilization of Yarrowia lipolytica Lipase on Macroporous Resin Using Different Methods: Characterization of the Biocatalysts in Hydrolysis Reaction.
abstract: To improve the reusability and organic solvent tolerance of microbial lipase and expand the application of lipase (hydrolysis, esterification, and transesterification), we immobilized marine microbial lipase using different methods and determined the properties of immobilized lipases. Considering the activity and cost of immobilized lipase, the concentration of lipase was fixed at 2 mg/mL. The optimal temperature of immobilized lipases was 40°C and 5°C higher than free lipase. The activities of immobilized lipases were much higher than free lipase at alkaline pH (more than 50% at pH 12). The free lipase lost most activity (35.3%) and immobilized lipases retained more than 46.4% of their initial activity after 3 h heat treatment at 70°C. At alkaline pH, immobilized lipases were more stable than free lipase (more than 60% residue activity at pH 11 for 3 h). Immobilized lipases retained 80% of their activity after 5 cycles and increased enzyme activity (more than 108.7%) after 3 h treatment in tert-butanol. Immobilization of lipase which improved reusability of lipase and provided a chance to expand the application of marine microbial lipase in organic system expanded the application range of lipase to catalyze hydrolysis and esterification in harsh condition.
authors.affiliation: Laboratory of Enzyme Engineering, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Laboratory of Enzyme Engineering, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Laboratory of Enzyme Engineering, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sheng
authors.affiliation: Laboratory of Enzyme Engineering, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Sun
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry*; D008422:Materials Testing; D016062:Porosity; D013379:Substrate Specificity; D025062:Yarrowia / Q000201:enzymology*
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D008049:Lipase