title: Development of Activity-Based Probes for Imaging Human α-l-Fucosidases in Cells.
abstract: We have established a concise synthetic route relying on a key base-promoted epimerization step to synthesize two series of activity-based probes carrying a BODIPY fluorophore for α-l-fucosidase. The resulting probes were evaluated for labeling performance. The one utilizing an o-fluoromethylphenol derivative as the latent trapping unit was successfully applied for the first time to visualize and locate lysosomal α-l-fucosidase activity in human cells.
authors.affiliation: †Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Hsu
authors.affiliation: ‡Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan.|§Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, No.128, Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan.|⊥Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Manjula
authors.initials: M
authors.lastname: Nandakumar
authors.affiliation: †Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Lai
authors.affiliation: †Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Tzyy-Chao
authors.initials: TC
authors.lastname: Chou
authors.affiliation: †Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chi-Yuan
authors.initials: CY
authors.lastname: Chu
authors.affiliation: †Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.|‡Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan.|§Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, No.128, Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan.|⊥Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Lin
authors.affiliation: †Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Lee-Chiang
authors.initials: LC
authors.lastname: Lo
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry* / Q000201:enzymology; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D010636:Phenols / Q000737:chemistry*; D005644:alpha-L-Fucosidase / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D010636:Phenols; D005644:alpha-L-Fucosidase