title: Functional characterization of FaNIP1;1 gene, a ripening-related and receptacle-specific aquaporin in strawberry fruit.
abstract: Strawberry fruit (Fragaria × ananassa) is a soft fruit with high water content at ripe stage (more than 90% of its fresh weight). Aquaporins play an important role in plant water homeostasis, through the facilitation of water transport and solutes. We report the role played by FaNIP1;1 in the receptacle ripening process. The analysis by qRT-PCR of FaNIP1;1 showed that this gene is mainly expressed in fruit receptacle and has a ripening-related expression pattern that was accompanied by an increase in both the abscisic acid and water content of the receptacle throughout fruit ripening. Moreover, FaNIP1;1 was induced in situations of water deficit. Additionally, we show that FaNIP1;1 expression was positively regulated by abscisic acid and negatively regulated by auxins. The water transport capacity of FaNIP1;1 was determined by a stopped-flow spectroscopy in yeast over-expressing FaNIP1;1. Glycerol, H2O2 and boron transport were also demonstrated in yeast. On the other hand, GFP-FaNIP1;1 fusion protein was located in plasma membrane. In conclusion, FaNIP1;1 seems to play an important role increasing the plasma membrane permeability, that allows the water accumulation in the strawberry fruit receptacle throughout the ripening process.
authors.affiliation: Department of Biochemistry and Molecular Biology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Molina-Hidalgo
authors.affiliation: Department of Biochemistry and Molecular Biology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Medina-Puche
authors.affiliation: Department of Microbiology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain.
authors.forename: Samuel
authors.initials: S
authors.lastname: Gelis
authors.affiliation: Department of Microbiology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Ramos
authors.affiliation: CBAA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-003, Portugal; Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003, Portugal.
authors.forename: Farzana
authors.initials: F
authors.lastname: Sabir
authors.affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003, Portugal; Departamento de Bioquímica e Biologia Humana, Faculdade de Farmácia, Universidade de Lisboa, 1649-003, Portugal.
authors.forename: Graça
authors.initials: G
authors.lastname: Soveral
authors.affiliation: CBAA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-003, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Prista
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Campus de Montegancedo, and E.T.S.I. Agrónomos, Universidad Politécnica de Madrid 28223, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Iglesias-Fernández
authors.affiliation: Department of Biochemistry and Molecular Biology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Caballero
authors.affiliation: Department of Biochemistry and Molecular Biology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain. Electronic address: bb1mublj@uco.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Muñoz-Blanco
authors.affiliation: Department of Biochemistry and Molecular Biology, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Blanco-Portales
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D020346:Aquaporins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D001895:Boron / Q000378:metabolism; D031985:Fragaria / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D005638:Fruit / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005990:Glycerol / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D009997:Osmotic Pressure / Q000187:drug effects; D010539:Permeability; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D007210:Indoleacetic Acids; D010940:Plant Proteins; D014867:Water; D008353:Mannitol; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D001895:Boron; D005990:Glycerol
keywords: Aquaporins; Fruit; Major intrinsic proteins; Ripening; Strawberry; Water transport