title: The Effects of the Selective Serotonin Reuptake Inhibitor Fluvoxamine on Voltage-Dependent K(+) Channels in Rabbit Coronary Arterial Smooth Muscle Cells.
abstract: We demonstrated the inhibitory effect of fluvoxamine, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent K(+) (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells using a whole-cell patch clamp technique. Fluvoxamine reduced the amplitude of Kv currents in a concentration-dependent manner with an IC50 value of 3.71±1.09 µM and a Hill coefficient of 0.62±0.14. Although fluvoxamine did not significantly affect the steady-state activation curve, it shifted the steady-state inactivation curve toward a more negative potential. Pretreatment with another SSRI, paroxetine, did not affect the basal Kv current and did not alter the inhibitory effect of fluvoxamine on Kv channels. We concluded that fluvoxamine inhibits the Kv current in a concentration-dependent manner and in a closed (inactivated) state of the Kv channels independent of serotonin reuptake inhibition.
authors.affiliation: Department of Physiology, Kangwon National University School of Medicine.
authors.forename: Da Hye
authors.initials: DH
authors.lastname: Hong
authors.forename: Hongliang
authors.initials: H
authors.lastname: Li
authors.forename: Han Sol
authors.initials: HS
authors.lastname: Kim
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Shin
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.forename: Sung Hun
authors.initials: SH
authors.lastname: Na
authors.forename: Il-Whan
authors.initials: IW
authors.lastname: Choi
authors.forename: Amy Leanne
authors.initials: AL
authors.lastname: Firth
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.forename: Dae-Joong
authors.initials: DJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016666:Fluvoxamine / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D011817:Rabbits; D017367:Selective Serotonin Reuptake Inhibitors / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D016666:Fluvoxamine; D024642:Potassium Channels, Voltage-Gated; D017367:Selective Serotonin Reuptake Inhibitors