title: Screening of Lactobacillus strains for their ability to produce conjugated linoleic acid in milk and to adhere to the intestinal tract.
abstract: Conjugated linoleic acid (CLA) has been shown to provide beneficial effects on health; however, the amount consumed in food is far from that required for the desired effects. Thus, increasing the CLA content in dairy foods through milk fermentation with specific lactic acid bacteria (LAB) offers an interesting alternative. Moreover, some LAB may be able to adhere to the intestinal mucosa and produce CLA through endogenous synthesis. Therefore, the objective of this study was to screen LAB isolates for their ability to produce CLA in skim milk and in simulated gastrointestinal conditions. Additionally, the ability of selected CLA-producing LAB to adhere to the intestinal mucosa in a murine model was assessed. Results showed that of 13 strains of Lactobacillus tested, only 4 were able to produce CLA in skim milk supplemented with linoleic acid (13.44 ± 0.78 to 50.9 ± 0.26 µg/mL). Furthermore, these 4 Lactobacillus strains were able to survive and produce CLA in simulated gastrointestinal conditions and to adhere to the intestinal mucosa of Wistar rats after 7 d of oral inoculation with fluorescently labeled bacteria. Accordingly, these 4 Lactobacillus strains may be used to manufacture fermented dairy foods to increase CLA content, and consumption of these fermented milks may result in CLA produced endogenously by these LAB.
authors.affiliation: Laboratorio de Química y Biotecnología de Productos Lácteos, Coordinación de Tecnología de Alimentos de Origen Animal, Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Sosa-Castañeda
authors.affiliation: Laboratorio de Química y Biotecnología de Productos Lácteos, Coordinación de Tecnología de Alimentos de Origen Animal, Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Mendoza
authors.affiliation: Laboratorio de Patología Experimental, Coordinación de Nutrición, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Hermosillo, Sonora 83304, Mexico.
authors.forename: H
authors.initials: H
authors.lastname: Astiazarán-García
authors.affiliation: Unidad de Investigación y Desarrollo en Alimentos (UNIDA) Instituto Tecnológico de Veracruz M. A. de Quevedo, Veracruz, Veracruz 91897, Mexico.
authors.forename: H S
authors.initials: HS
authors.lastname: Garcia
authors.affiliation: Laboratorio de Química y Biotecnología de Productos Lácteos, Coordinación de Tecnología de Alimentos de Origen Animal, Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
authors.forename: M C
authors.initials: MC
authors.lastname: Estrada-Montoya
authors.affiliation: Laboratorio de Química y Biotecnología de Productos Lácteos, Coordinación de Tecnología de Alimentos de Origen Animal, Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
authors.forename: A F
authors.initials: AF
authors.lastname: González-Córdova
authors.affiliation: Laboratorio de Química y Biotecnología de Productos Lácteos, Coordinación de Tecnología de Alimentos de Origen Animal, Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico. Electronic address: vallejo@ciad.mx.
authors.forename: B
authors.initials: B
authors.lastname: Vallejo-Cordoba
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D043302:Cultured Milk Products / Q000737:chemistry; D005285:Fermentation; D007421:Intestine, Small / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics / Q000502:physiology*; D044243:Linoleic Acids, Conjugated / Q000378:metabolism*; D008297:Male; D008892:Milk / Q000737:chemistry* / Q000382:microbiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar / Q000378:metabolism / Q000382:microbiology
chemical_list: D044243:Linoleic Acids, Conjugated
keywords: Lactobacillus; conjugated linoleic acid; fermented milk; lactic acid bacteria