title: Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering.
abstract: The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu 136-701, Republic of Korea.
authors.forename: Kyu-Sik
authors.initials: KS
authors.lastname: Shim
authors.affiliation: Division of Bio-imaging, Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Choi
authors.affiliation: Department of Orthopedic Surgery, Korea University Medical College, Ansan Hospital, Gojan 1-dong, Danwon-gu, Gyeonggi-do 425-707, Republic of Korea.
authors.forename: Dong Hyup
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Department of Orthopedic Surgery, Korea University Medical College, Ansan Hospital, Gojan 1-dong, Danwon-gu, Gyeonggi-do 425-707, Republic of Korea. Electronic address: mayhap@hanmail.net.
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Suh
mesh_terms: D001846:Bone Development; D002460:Cell Line; D020869:Gene Expression Profiling; D006801:Humans; D008863:Microspheres; D023822:Tissue Engineering
keywords: Bone morphogenic protein-2 (BMP-2); Bone tissue engineering; Fluidic device; Heparin; Porous microspheres