title: Interferon regulatory factor 10 (IRF10): Cloning in orange spotted grouper, Epinephelus coioides, and evolutionary analysis in vertebrates.
abstract: IRF10 gene was cloned in orange spotted grouper, Epinephelus coioides, and its expression was examined following poly(I:C) stimulation and bacterial infection. The cDNA sequence of grouper IRF10 contains an open reading frame of 1197 bp, flanked by 99 bp 5'-untranslated region and 480 bp 3'- untranslated region. Multiple alignments showed that the grouper IRF10 has a highly conserved DNA binding domain in the N terminus with characteristic motif containing five tryptophan residues. Quantitative real-time PCR analysis revealed that the expression of IRF10 was responsive to both poly(I:C) stimulation and Vibrio parahemolyticus infection, with a higher increase to poly(I:C), indicating an important role of IRF10 in host immune response during infection. A phyletic distribution of IRF members was also examined in vertebrates, and IRF10 was found in most lineages of vertebrates, not in modern primates and rodents. It is suggested that the first divergence of IRF members might have occurred before the evolutionary split of vertebrate and cephalochordates, producing ancestors of IRF (1/2/11) and IRF (4/8/9/10)[(3/7) (5/6)], and that the second and/or third divergence of IRF members occurred following the split, thus leading to the subsets of the IRF family in vertebrates.
authors.affiliation: College of Fisheries, Jimei University, 43 Yindou Road, Xiamen, Fujian Province, 361021, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: College of Fisheries, Jimei University, 43 Yindou Road, Xiamen, Fujian Province, 361021, China.
authors.forename: Qin Qin
authors.initials: QQ
authors.lastname: Jia
authors.affiliation: College of Fisheries, Jimei University, 43 Yindou Road, Xiamen, Fujian Province, 361021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: College of Fisheries, Jimei University, 43 Yindou Road, Xiamen, Fujian Province, 361021, China.
authors.forename: Wen Shu
authors.initials: WS
authors.lastname: Huang
authors.affiliation: College of Fisheries, Jimei University, 43 Yindou Road, Xiamen, Fujian Province, 361021, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China. Electronic address: pinnie@ihb.ac.cn.
authors.forename: P
authors.initials: P
authors.lastname: Nie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001492:Bass; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D050835:Interferon Regulatory Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment / Q000662:veterinary; D014714:Vertebrates / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D030161:Avian Proteins; D029941:Fish Proteins; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger; C455582:interferon regulatory factor-10, Gallus gallus; D011070:Poly I-C
keywords: Epinephelus coioides; Evolution; IRF10; Orange spotted grouper; Vertebrate