title: Characterization of a Novel Putative Xer-Dependent Integrative Mobile Element Carrying the bla(NMC-A) Carbapenemase Gene, Inserted into the Chromosome of Members of the Enterobacter cloacae Complex.
abstract: An Enterobacter ludwigii strain was isolated during routine screening of a Japanese patient for carriage of carbapenem-resistant Enterobacteriaceae. PCR analysis revealed the blaNMC-A carbapenemase gene. Whole-genome sequencing revealed that blaNMC-A was inserted in the chromosome and associated with a novel 29.1-kb putative Xer-dependent integrative mobile element, named EludIMEX-1. Bioinformatic analysis identified similar elements in the genomes of an Enterobacter asburiae strain and of other Enterobacter cloacae complex strains, confirming the mobile nature of this element.
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy Department of Medical Biotechnology, University of Siena, Siena, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Antonelli
authors.affiliation: Department of Medical Biotechnology, University of Siena, Siena, Italy.
authors.forename: Marco Maria
authors.initials: MM
authors.lastname: D'Andrea
authors.affiliation: Department of Medical Biotechnology, University of Siena, Siena, Italy Department of Surgery and Translational Medicine, University of Florence, Florence, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Di Pilato
authors.affiliation: Neurointensive Care Unit, Florence Careggi University Hospital, Florence, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Viaggi
authors.affiliation: Genetic Diagnostic Unit, Florence Careggi University Hospital, Florence, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Torricelli
authors.affiliation: Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy Department of Medical Biotechnology, University of Siena, Siena, Italy Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy gianmaria.rossolini@unisi.it.
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D016972:Enterobacter cloacae / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase