title: Feeding regulates sex pheromone attraction and courtship in Drosophila females.
abstract: In Drosophila melanogaster, gender-specific behavioural responses to the male-produced sex pheromone cis-vaccenyl acetate (cVA) rely on sexually dimorphic, third-order neural circuits. We show that nutritional state in female flies modulates cVA perception in first-order olfactory neurons. Starvation increases, and feeding reduces attraction to food odour, in both sexes. Adding cVA to food odour, however, maintains attraction in fed females, while it has no effect in males. Upregulation of sensitivity and behavioural responsiveness to cVA in fed females is paralleled by a strong increase in receptivity to male courtship. Functional imaging of the antennal lobe (AL), the olfactory centre in the insect brain, shows that olfactory input to DA1 and VM2 glomeruli is also modulated by starvation. Knocking down insulin receptors in neurons converging onto the DA1 glomerulus suggests that insulin-signalling partly controls pheromone perception in the AL, and adjusts cVA attraction according to nutritional state and sexual receptivity in Drosophila females.
authors.affiliation: 1] Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Division of Chemical Ecology, Alnarp, Sweden [2] Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lebreton
authors.affiliation: 1] Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Division of Chemical Ecology, Alnarp, Sweden [2] Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Federica
authors.initials: F
authors.lastname: Trona
authors.affiliation: 1] Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Division of Chemical Ecology, Alnarp, Sweden [2] Colombian Corporation of Agricultural Research Research CORPOICA, Biological Control Laboratory, Las Palmas 240142, Colombia.
authors.forename: Felipe
authors.initials: F
authors.lastname: Borrero-Echeverry
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Bilz
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Veit
authors.initials: V
authors.lastname: Grabe
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Division of Chemical Ecology, Alnarp, Sweden.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Becher
authors.affiliation: Department of Zoology, Stockholm University, Stockholm, Sweden.
authors.forename: Mikael A
authors.initials: MA
authors.lastname: Carlsson
authors.affiliation: Department of Zoology, Stockholm University, Stockholm, Sweden.
authors.forename: Dick R
authors.initials: DR
authors.lastname: Nässel
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Bill S
authors.initials: BS
authors.lastname: Hansson
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Sachse
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Division of Chemical Ecology, Alnarp, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Witzgall
mesh_terms: D000085:Acetates / Q000737:chemistry / Q000494:pharmacology*; D019342:Acetic Acid / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000502:physiology*; D005247:Feeding Behavior / Q000187:drug effects*; D005260:Female; D007328:Insulin / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000378:metabolism; D009829:Oleic Acids / Q000737:chemistry / Q000494:pharmacology*; D010675:Pheromones / Q000737:chemistry / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D011972:Receptor, Insulin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism; D012724:Sex Attractants / Q000737:chemistry / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D012903:Smell / Q000502:physiology; D013217:Starvation
chemical_list: D000085:Acetates; D029721:Drosophila Proteins; D007328:Insulin; D009829:Oleic Acids; D010675:Pheromones; D034741:RNA, Small Interfering; D018035:Receptors, Odorant; D012724:Sex Attractants; C505385:cis-vaccenyl acetate; D011972:Receptor, Insulin; D019342:Acetic Acid