title: Real-Time Drug Release Analysis of Enzyme and pH Responsive Polysaccharide Nanovesicles.
abstract: The accurate estimation of drug release kinetics of polymeric vehicles is an indispensable prerequisite for the developments of successful drug carriers for cancer therapy. The present investigation reports the development of time-resolved fluorescence spectroscopic approach for the real-time release kinetics of fluorophore loaded polysaccharide vesicles that are potential vectors in cancer treatment. The polysaccharide vesicles were custom designed with appropriate enzyme and pH responsiveness and loaded with water-soluble biocompatible fluorophore Rhodamine B (Rh-B). The semipermeable membrane dialysis method along with steady state absorbance spectroscopic technique was found to be inaccurate for the estimation of drug release. Time correlated single photon counting (TCSPC) technique was found to exhibit significant difference in excited state decay profiles and fluorescent lifetime of Rh-B in the free and polymer bound states. This enabled the establishment of real-time drug release protocols by TCSPC method for polysaccharide vesicles that are responsible to pH and enzyme with respect to intracellular compartments. Real-time analysis predicted the release kinetics 20-25% higher accuracy when compared to the dialysis method under in vitro conditions. Moreover, the ability of enzyme to cleave the polysaccharide vesicles was further validated by docking studies. The positioning of the molecules in active site of enzyme and the binding energy data were generated using AUTODOCK program to study the rupture of polysaccharide vesicles. This new TCSPC technique could be very useful for studying the drug release pattern of synthetic polymer vesicles loaded with Rh-B fluorophore.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER)-Pune, Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India.
authors.forename: Poothayil Subash
authors.initials: PS
authors.lastname: Pramod
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER)-Pune, Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India.
authors.forename: Nilesh Umakant
authors.initials: NU
authors.lastname: Deshpande
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER)-Pune, Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India.
authors.forename: Manickam
authors.initials: M
authors.lastname: Jayakannan
mesh_terms: D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D045744:Cell Line, Tumor; D003911:Dextrans / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D062105:Molecular Docking Simulation; D049329:Nanostructures; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D003911:Dextrans; D004337:Drug Carriers; D011134:Polysaccharides; D012235:Rhodamines; D014867:Water; D004317:Doxorubicin; D002265:Carboxylic Ester Hydrolases; C474609:CES1 protein, human; C029773:rhodamine B