title: Specific miRNA and its target in neutrophils after traumatic injury.
abstract: Traumatic injury is a leading cause of mortality and morbidity. MicroRNAs (miRNAs) regulate the cellular responses when traumatic injury occurs. Previously, we reported that miR-3945, miR-125a-5p, miR-363-3p, and miR-150-5p were significantly altered in neutrophils of patients who suffered traumatic injury. In the present study, by comparing neutrophils of patients suffering from major trauma with neutrophils of patients with a inflammatory disease, we found that the variation trend of miR-150-5p was relatively different in the process of these two diseases. Gene Ontology and pathway analysis of miR-150-5p revealed that it may activate the mitogen-activated protein kinase and Toll-like receptor signaling pathways and cell adhesion molecules when the traumatic injury occurs. In addition, protein kinase C alpha (PRKCA) was also identified as a direct target of miR-150-5p by establishing a miRNA-mRNA network, and this target was validated via dual-luciferase reporter and western blot analysis. Our results suggested that the expression of miR-150-5p was down-regulated in neutrophils after a major traumatic injury. miR-150-5p and its identified target PRKCA play important roles in the development of traumatic process.
authors.affiliation: Department of Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
authors.forename: Huazhong
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China qinhl126@126.com.
authors.forename: Huanlong
authors.initials: H
authors.lastname: Qin
mesh_terms: D019295:Computational Biology; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism; D014947:Wounds and Injuries / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha
keywords: PRKCA; bioinformatics; microRNA; neutrophils; traumatic injury