title: Magnetic zirconium hexacyanoferrate(II) nanoparticle as tracing tag for electrochemical DNA assay.
abstract: Novel multifunctional magnetic zirconium hexacyanoferrate nanoparticles (ZrHCF MNPs) were prepared, which consisted of magnetic beads (MBs) inner core and zirconium hexacyanoferrate(II) (ZrHCF) outer shell. As an artificial peroxidase, the ZrHCF MNPs exhibited remarkable electrocatalytic properties in the reduction of H2O2 at 0.2 V vs saturated calomel electrode (SCE). On the basis of the bonding interaction between Zr (IV) of the shell ZrHCF framework and phosphonate groups, the 5'-phosphorylated ssDNA probes with a consecutive stretch of guanines as a spacer could be incorporated in ZrHCF MNPs easily. Thus, DNA-grafted ZrHCF MNPs could be simply obtained by magnetic separation. The prepared nanoelectrocatalyst was further used as signal nanoprobe for the ultrasensitive electrochemical DNA assay. Under optimal conditions, the proposed biosensor presents high sensitivity for detecting target DNA with a linear range from 1.0 fM to 1.0 nM and a low detection limit of 0.43 fM. Moreover, it exhibits good performance with excellent selectivity, high stability, and acceptable fabrication reproducibility.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu 210094, China.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu 210094, China.
authors.forename: Sheng-Yuan
authors.initials: SY
authors.lastname: Deng
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu 210094, China.
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Cai
authors.affiliation: University of Grenoble Alpes-CNRS , DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu 210094, China.
authors.forename: Xue-Ji
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu 210094, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
mesh_terms: D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D005295:Ferrocyanides / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry*
chemical_list: D005295:Ferrocyanides; D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D004247:DNA; D015040:Zirconium; C020354:hexacyanoferrate II