title: In Situ Hybridization to Identify Gut Stem Cells.
abstract: In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.
authors.affiliation: Hubrecht Institute, Utrecht, The Netherlands.
authors.forename: Alex
authors.initials: A
authors.lastname: Gregorieff
authors.affiliation: Hubrecht Institute, Utrecht, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology*; D017403:In Situ Hybridization / Q000379:methods*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D051379:Mice; D051381:Rats
keywords: (ISH) in situ hybridization; digoxigenin RNA probes; formalin-fixed paraffin-embedded; intestinal sections