title: Adhesion Properties of Human Oral Epithelial-Derived Cells to Zirconia.
abstract: BACKGROUND
  Few studies have examined epithelial attachment to zirconia and the proliferative ability of epithelial cells on zirconia surfaces.
  
  
  OBJECTIVE
  To evaluate the adhesion properties of zirconia materials for epithelial cell attachment and compare this with titanium and alumina.
  
  
  METHODS
  Human oral epithelial cells were cultured on smooth-surfaced specimens of commercially pure titanium (cpTi), ceria-stabilized zirconia/alumina nano-composite (P-NANOZR), yttria-stabilized zirconia (Cercon), and alumina oxide (inCoris AL). The cell morphology, the cell viability and mRNA of integrin β4, laminin γ2, catenin δ2, and E-cadherin were evaluated by SEM, Cell-Counting Kit-8, and real-time PCR, respectively.
  
  
  RESULTS
  Morphology of cells attached to specimens was similar among all groups. The viable cell numbers on Cercon and inCoris AL after 24 hours culture were significantly higher than for cpTi. Integrin β4, laminin γ2, and catenin δ2mRNA expression was not different among all groups. However, at 3 and 24 hours after incubation, E-cadherin mRNA expression in the P-NANOZR group was significantly higher than for cpTi.
  
  
  CONCLUSIONS
  Zirconia may support binding of epithelial cells through hemidesmosomes comparable with titanium. Furthermore, P-NANOZR may impart resistance to exogenous stimuli through strong intercellular contacts with peri-implant mucosal cells when used as an abutment and implant superstructure.
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Eijiro
authors.initials: E
authors.lastname: Okabe
authors.affiliation: Department of Operative Dentistry, Endodontology, and Periodontology, School of Dentistry, Matsumoto Dental University, Nagano, Japan. ishiharay@po.mdu.ac.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ishihara
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kikuchi
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Ario
authors.initials: A
authors.lastname: Izawa
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Goto
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kamiya
authors.affiliation: Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Ban
authors.affiliation: School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Noguchi
authors.affiliation: Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Mitani
mesh_terms: D015820:Cadherins / Q000235:genetics; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D015040:Zirconium
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; D015040:Zirconium; C028541:zirconium oxide
keywords: abutments; epithelial attachment; titanium; zirconia