title: Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines.
abstract: Primary cilia (PC) were demonstrated for the first time in fish endothelial, epithelial and fibroblast cell lines through immunofluorescence staining with the monoclonal antibody, 6-11B-1, against acetylated α-tubulin. The study was carried out with eight recently developed cell lines from the walleye, Sander vitreus (Mitchill). These were three fibroblast-like cell lines, WE-cfin11f, WE-skin11f and WE-liver3 from, respectively, the caudal fin, skin and liver, and three epithelial-like cell lines, WE-cfin11e, WE-spleen6 and WErpe from, respectively, the caudal fin, spleen and retina. Also, endothelial-like WEBA from the bulbus arteriosus and glial-like WE-brain5 from the brain were used. Immunocytochemistry revealed strong staining for acetylated α-tubulin in mitotic spindles and midbodies for all cell lines, and in PC for all cell lines except WE-skin11f. Staining of cytoplasmic microtubules (fibrils) was absent in three cell lines, including WEBA, but present in the others, especially WE-skin11f, which might have obscured PC detection in these cells. Tubacin, an inhibitor of histone deacetylase 6, induced cytoplasmic fibrils in WEBA and the intensity of their staining in WE-cfin11f. These results suggest that the cell lines might differ in their deacetylase activities, making them useful for studying this tubulin modification in teleosts, as well as for studying PC.
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada. nathanntkvo@gmail.com.
authors.forename: Nguyen T K
authors.initials: NT
authors.lastname: Vo
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada. ncbols@uwaterloo.ca.
authors.forename: Niels C
authors.initials: NC
authors.lastname: Bols
mesh_terms: D000818:Animals; D002460:Cell Line; D002923:Cilia / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D010471:Perches; D014404:Tubulin / Q000378:metabolism*
chemical_list: D014404:Tubulin
keywords: Acetylated α-tubulin; Brain; Cell lines; Microtubules; Primary cilia; Walleye