title: Evolution and Conservation on Top of the World: Phylogeography of the Marbled Water Frog (Telmatobius marmoratus Species Complex; Anura, Telmatobiidae) in Protected Areas of Chile.
abstract: The Andean Altiplano has served as a complex setting throughout its history, driving dynamic processes of diversification in several taxa. We investigated phylogeographic processes in the Telmatobius marmoratus species complex occurring in this region by studying the geographic patterns of genetic variability, genealogies, and historical migration, using the cytochrome b (cyt-b) gene as a marker. DNA sequences from Telmatobius gigas and Telmatobius culeus, Bolivian species with an uncertain taxonomic status, were also included. Additionally, we evaluated the phylogenetic diversity (PD) represented within Chilean protected areas and the complementary contribution from unprotected populations. Phylogenetic reconstructions from 148 cyt-b sequences revealed 4 main clades, one of which corresponded to T. culeus. T. gigas was part of T. marmoratus clade indicating paraphyletic relationships. Haplotypes from Chilean and Bolivian sites were not reciprocally monophyletic. Geographic distribution of lineages, spatial Bayesian analysis, and migration patterns indicated that T. marmoratus displays a weaker geographic structure than expected based on habitat distribution and physiological requirements. Demographic and statistical phylogeography analyses pointed out to a scenario of recent population expansion and high connectivity events of a more recent age than the post Last Glacial Maximum, probably associated to more humid events in Altiplano. PD of T. marmoratus populations within protected areas represents 55.6% of the total estimated PD. The unprotected populations that would contribute the most to PD are Caquena and Quebe (21%). Recent evolutionary processes and paleoclimatic changes, potentially driving shifts in habitat connectivity levels and population sizes, could explain the phylogeographic patterns recovered herein.
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez). pvictori@gmail.com.
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Victoriano
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Carla
authors.initials: C
authors.lastname: Muñoz-Mendoza
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Paola A
authors.initials: PA
authors.lastname: Sáez
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Hugo F
authors.initials: HF
authors.lastname: Salinas
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Carlos
authors.initials: C
authors.lastname: Muñoz-Ramírez
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Michel
authors.initials: M
authors.lastname: Sallaberry
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Pablo
authors.initials: P
authors.lastname: Fibla
authors.affiliation: From the Depto. de Zoología, Fac. de Cs. Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile (Victoriano and Muñoz-Mendoza); Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile, Santiago, Chile (Sáez, Salinas, Sallaberry, Fibla, and Méndez); Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA (Muñoz-Ramírez).
authors.forename: Marco A
authors.initials: MA
authors.lastname: Méndez
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics*; D001499:Bayes Theorem; D001838:Bolivia; D002677:Chile; D003247:Conservation of Natural Resources; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D005828:Genetics, Population; D006239:Haplotypes; D008957:Models, Genetic; D010802:Phylogeny; D058974:Phylogeography; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b
keywords: Andean Altiplano; Chile; Phylogenetic Diversity; Telmatobius