title: Conjugated Polymer Nanoparticles for Fluorescence Imaging and Sensing of Neurotransmitter Dopamine in Living Cells and the Brains of Zebrafish Larvae.
abstract: Nanoscale materials are now attracting a great deal of attention for biomedical applications. Conjugated polymer nanoparticles have remarkable photophysical properties that make them highly advantageous for biological fluorescence imaging. We report on conjugated polymer nanoparticles with phenylboronic acid tags on the surface for fluorescence detection of neurotransmitter dopamine in both living PC12 cells and brain of zebrafish larvae. The selective enrichment of dopamine and fluorescence signal amplification characteristics of the nanoparticles show rapid and high-sensitive probing such neurotransmitter with the detection limit of 38.8 nM, and minimum interference from other endogenous molecules. It demonstrates the potential of nanomaterials as a multifunctional nanoplatform for targeting, diagnosis, and therapy of dopamine-relative disease.
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Cheng-Gen
authors.initials: CG
authors.lastname: Qian
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Pei-Jian
authors.initials: PJ
authors.lastname: Feng
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Yu-Lei
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Yu
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Polymer Science & Engineering and Key Laboratory of High Performance Polymer Materials & Technology of MOE, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Qun-Dong
authors.initials: QD
authors.lastname: Shen
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D000067493:Dynamic Light Scattering; D005456:Fluorescent Dyes / Q000737:chemistry; D007814:Larva / Q000378:metabolism; D008027:Light; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D016716:PC12 Cells; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D011108:Polymers; C010686:benzeneboronic acid; D004298:Dopamine
keywords: bioimaging; conjugated polymers; dopamine; fluorescence sensing; nanoparticles