title: Simultaneous production of lactobionic and gluconic acid in cheese whey/glucose co-fermentation by Pseudomonas taetrolens.
abstract: Substrate versatility of Pseudomonas taetrolens was evaluated for the first time in a co-fermentation system combining cheese whey and glucose, glycerol or lactose as co-substrates. Results showed that P. taetrolens displayed different production patterns depending on the co-substrate supplied. Whereas the presence of glucose led to a simultaneous co-production of lactobionic (78g/L) and gluconic acid (8.8g/L), lactose feeding stimulated the overproduction of lactobionic acid from whey with a high specific productivity (1.4g/gh) and yield (100%). Co-substrate supply of glycerol conversely led to reduced lactobionic acid yield (82%) but higher cell densities (1.8g/L), channelling the carbon source towards cell growth and maintenance. Higher carbon availability impaired the metabolic activity as well as membrane integrity, whereas lactose feeding improved the cellular functionality of P. taetrolens. Insights into these mixed carbon source strategies open up the possibility of co-producing lactobionic and gluconic acid into an integrated single-cell biorefinery.
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Chemistry, University of Oviedo, C/Julián Clavería s/n, 33071 Oviedo, Spain.
authors.forename: Saúl
authors.initials: S
authors.lastname: Alonso
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Chemistry, University of Oviedo, C/Julián Clavería s/n, 33071 Oviedo, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Rendueles
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Chemistry, University of Oviedo, C/Julián Clavería s/n, 33071 Oviedo, Spain. Electronic address: mariodiaz@uniovi.es.
authors.forename: Mario
authors.initials: M
authors.lastname: Díaz
mesh_terms: D002611:Cheese; D004187:Disaccharides / Q000378:metabolism*; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D000067796:Whey; D000067816:Whey Proteins
chemical_list: D004187:Disaccharides; D005942:Gluconates; D000067816:Whey Proteins; C005608:lactobionic acid; D005947:Glucose; D007785:Lactose; C030691:gluconic acid
keywords: Cheese whey; Co-fermentation; Co-production system; Gluconic acid; Lactobionic acid