title: Development and evaluation of PCR methods based on cytochrome c oxidase subunit one (cox1) and NADH dehydrogenase subunit one gene (nad1) to detect Opisthorchis viverrini in human fecal samples.
abstract: Opisthorchis viverrini is highly prevalent throughout Southeast Asia. Chronic infection of this parasite leads to cholangiocarcinoma (CCA), a fatal bile duct cancer. The early and accurate detection of this parasite is very important; therefore, new PCR methods targeting cytochrome c oxidase subunit one and NADH dehydrogenase subunit one gene to detect O. viverrini in fecal specimens have been developed. Ninety O. viverrini-positive human fecal samples were used in this study. The PCR sensitivity of both genes was compared with internal transcribe spacer 2 (ITS2)-PCR. The sensitivity of cox1-PCR and nad1-PCR was 66.7 and 50 %, respectively. The sensitivity of cox1-PCR and nad1-PCR achieved 89.1 and 71.7 % in specimens containing O. viverrini eggs of >100 eggs per gram (EPG). Additionally, these primers can be used to provide the information on genetic diversity from mitochondrial genes of O. viverrini.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Saiwasan
authors.initials: S
authors.lastname: Buathong
authors.forename: Saovanee
authors.initials: S
authors.lastname: Leelayoova
authors.forename: Mathirut
authors.initials: M
authors.lastname: Mungthin
authors.forename: Tawee
authors.initials: T
authors.lastname: Naaglor
authors.forename: Paanjit
authors.initials: P
authors.lastname: Taamasri
authors.forename: Picha
authors.initials: P
authors.lastname: Suwannahitatorn
authors.forename: Peerapan
authors.initials: P
authors.lastname: Tan-Ariya
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000209:etiology*; D018281:Cholangiocarcinoma / Q000209:etiology*; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D009245:NADH Dehydrogenase / Q000235:genetics*; D009889:Opisthorchiasis / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013785:Thailand
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D015801:Helminth Proteins; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV