title: Assessment of polycarbonate filter in a molecular analytical system for the microbiological quality monitoring of recycled waters onboard ISS.
abstract: On the ISS, as on Earth, water is an essential element for life and its quality control on a regular basis allows to ensure the health of the crew and the integrity of equipment. Currently, microbial water analysis onboard ISS still relies on the traditional culture-based microbiology methods. Molecular methods based on the amplification of nucleic acids for microbiological analysis of water quality show enormous potential and are considered as the best alternative to culture-based methods. For this reason, the Midass, a fully integrated and automated prototype was designed conjointly by ESA and bioMérieux for a rapid monitoring of the microbiological quality of air. The prototype allows air sampling, sample processing and the amplification/detection of nucleic acids. We describe herein the proof of principle of an analytical approach based on molecular biology that could fulfill the ESA's need for a rapid monitoring of the microbiological quality of recycled water onboard ISS. Both concentration and recovery of microorganisms are the main critical steps when the microfiltration technology is used for water analysis. Among filters recommended standards for monitoring the microbiological quality of the water, the polycarbonate filter was fully in line with the requirements of the ISO 7704-1985 standard in terms of efficacy of capture and recovery of bacteria. Moreover, this filter does not retain nucleic acids on the surface and has no inhibitory effect on their downstream processing steps such as purification and amplification/detection. Although the Midass system was designed for the treatment of air samples, the first results on the integration of PC filters were encouraging. Nevertheless, system modifications are needed to better adapt the Midass system for the monitoring of the microbiological water quality.
authors.affiliation: bioMérieux S.A., Chemin de L'Orme, 69280 Marcy l'Etoile, France; Université Claude Bernard Lyon 1, UMR CNRS 5558 - LBBE, Bâtiment Mendel 43 Bd du 11 novembre 1918, 69622 Villeurbanne, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Bechy-Loizeau
authors.affiliation: Université Claude Bernard Lyon 1, UMR CNRS 5558 - LBBE, Bâtiment Mendel 43 Bd du 11 novembre 1918, 69622 Villeurbanne, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Flandrois
authors.affiliation: bioMérieux S.A., Chemin de L'Orme, 69280 Marcy l'Etoile, France. Electronic address: hafid.abaibou@biomerieux.com.
authors.forename: Hafid
authors.initials: H
authors.lastname: Abaibou
mesh_terms: D000391:Air Microbiology; D019117:Burkholderia / Q000235:genetics*; D002176:Candida albicans / Q000235:genetics*; D018504:Ecological Systems, Closed; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005118:Extraterrestrial Environment; D008851:Micropore Filters / Q000382:microbiology; D058889:Molecular Typing / Q000379:methods*; D052638:Particulate Matter / Q000032:analysis*; D011076:Polycarboxylate Cement / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D059027:Recycling; D013026:Space Flight; D018531:Spacecraft; D014867:Water / Q000032:analysis*; D014871:Water Microbiology
chemical_list: D052638:Particulate Matter; D011076:Polycarboxylate Cement; D014867:Water; C005506:polycarbonate
keywords: Bio-contamination; Microfiltration; NASBA; RT-PCR; Water recycling; rRNA