title: Chlorpyrifos Determined in Human Blood by UPLC-MS/MS and Its Application in Poisoning Cases.
abstract: OBJECTIVE
  To determine the chlorpyrifos in human blood by liquid chromatography-tandem mass spectrometry and to validate its application in poisoning cases.
  
  
  METHODS
  The samples were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Capcell Pack C18 MGII column (250 mm x 2.0 mm, 5 μm) using an isocratic elution of solvent A (0.1% formic acid-water with 2 mmol/L ammonium acetate) and solvent B (methanol with 2 mmol/L ammonium acetate) at 5:95 V:V).
  
  
  RESULTS
  The linear ranged from 5 to 500 ng/mL (r = 0.998 7). The limit of detection (LOD) and the lower limit of quantification (LLOQ) were 2 ng/mL and 4 ng/mL, respectively. For this method, the precision and accuracy of intra-day and inter-day were < 10% and 97.44%-101.10%, respectively. The results in stability test of long-term frozen were satisfied. The matrix effect, recovery and process efficiency were 64.97%-86.81%, 76.70%-85.52%, and 55.57%-66.58%, respectively.
  
  
  CONCLUSIONS
  This method can provide a rapid approach to chlorpyrifos extraction and determination in toxicological analysis of forensic and clinical treatment.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Qiao
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Xian-yi
authors.initials: XY
authors.lastname: Zhuo
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Shen
mesh_terms: D004390:Chlorpyrifos / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D011041:Poisoning; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004390:Chlorpyrifos