title: A fungal monooxygenase-derived jasmonate attenuates host innate immunity.
abstract: Distinct modifications fine-tune the activity of jasmonic acid (JA) in regulating plant growth and immunity. Hydroxylated JA (12OH-JA) promotes flower and tuber development but prevents induction of JA signaling, plant defense or both. However, biosynthesis of 12OH-JA has remained elusive. We report here an antibiotic biosynthesis monooxygenase (Abm) that converts endogenous free JA into 12OH-JA in the model rice blast fungus Magnaporthe oryzae. Such fungal 12OH-JA is secreted during host penetration and helps evade the defense response. Loss of Abm in M. oryzae led to accumulation of methyl JA (MeJA), which induces host defense and blocks invasive growth. Exogenously added 12OH-JA markedly attenuated abmΔ-induced immunity in rice. Notably, Abm itself is secreted after invasion and most likely converts plant JA into 12OH-JA to facilitate host colonization. This study sheds light on the chemical arms race during plant-pathogen interaction, reveals Abm as an antifungal target and outlines a synthetic strategy for transformation of a versatile small-molecule phytohormone.
authors.affiliation: Temasek Life Sciences Laboratory, Singapore.
authors.forename: Rajesh N
authors.initials: RN
authors.lastname: Patkar
authors.affiliation: 1] Department of Biological Sciences, National University of Singapore, Singapore. [2] Singapore Centre for Environmental Life Sciences Engineering, Singapore. [3] NUS Environmental Research Institute, National University of Singapore, Singapore.
authors.forename: Peter I
authors.initials: PI
authors.lastname: Benke
authors.affiliation: 1] Temasek Life Sciences Laboratory, Singapore. [2] Department of Biological Sciences, National University of Singapore, Singapore.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Temasek Life Sciences Laboratory, Singapore.
authors.forename: Yuan Yi Constance
authors.identifier: 000000021279451X
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Temasek Life Sciences Laboratory, Singapore.
authors.forename: Fan
authors.identifier: 0000000296643953
authors.initials: F
authors.lastname: Yang
authors.affiliation: 1] Department of Biological Sciences, National University of Singapore, Singapore. [2] Singapore Centre for Environmental Life Sciences Engineering, Singapore. [3] NUS Environmental Research Institute, National University of Singapore, Singapore.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Swarup
authors.affiliation: 1] Temasek Life Sciences Laboratory, Singapore. [2] Department of Biological Sciences, National University of Singapore, Singapore. [3] School of Biological Sciences, Nanyang Technological University, Singapore.
authors.forename: Naweed I
authors.initials: NI
authors.lastname: Naqvi
mesh_terms: D003517:Cyclopentanes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015966:Gene Expression Regulation, Fungal; D054884:Host-Pathogen Interactions / Q000276:immunology; D006900:Hydroxylation; D020082:Magnaporthe / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D008745:Methylation; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008958:Models, Molecular; D012275:Oryza / Q000276:immunology* / Q000382:microbiology; D054883:Oxylipins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D010937:Plant Growth Regulators / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D057865:Plant Immunity; D018515:Plant Leaves / Q000276:immunology / Q000382:microbiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction
chemical_list: D003517:Cyclopentanes; D005656:Fungal Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; D011994:Recombinant Proteins; C011006:jasmonic acid; D006899:Mixed Function Oxygenases