title: Ultrastructural analysis of olfactory ensheathing cells derived from olfactory bulb and nerve of neonatal and juvenile rats.
abstract: Olfactory nerve derived and olfactory bulb derived olfactory ensheathing cells (OECs) have the ability to promote axonal regeneration and remyelination, both of which are essential in a successful cell transplant. Thus, morphological identification of OECs is a key aspect to develop an applicable cell therapy for injuries to the nervous system. However, there is no clear definition regarding which developmental stage or anatomical origin of OECs is more adequate for neural repair. In the present study, an ultrastructural comparison was made between OECs recovered from primary cultures of olfactory nerve and bulb in two developmental stages. The most notorious difference between cells obtained from olfactory nerve and bulb was the presence of indented nuclei in bulb derived OECs, suggesting a greater ability for possible chemotaxis. In neonatal OECs abundant mitochondria, lipid vacuoles, and smooth endoplasmic reticulum were detected, suggesting an active lipid metabolism, probably involved in synthesis of myelin. Our results suggest that neonatal OECs obtained from olfactory bulb have microscopic properties that could make them more suitable for neural repair.
authors.affiliation: Facultad de Medicina, Universidad de La Sabana, Campus Universitario Puente del Común, Km 7 Autopista Norte, Chía, Colombia; Fundación Neuroregeneración de Colombia, Colombia. Electronic address: rosa.gomez@unisabana.edu.co.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Gómez
authors.affiliation: Facultad de Medicina, Universidad de La Sabana, Campus Universitario Puente del Común, Km 7 Autopista Norte, Chía, Colombia; Unidad de Neurocirugía, Fundación Santafé de Bogotá, Colombia.
authors.forename: Kemel
authors.initials: K
authors.lastname: Ghotme
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional de Colombia, Colombia.
authors.forename: Lucía
authors.initials: L
authors.lastname: Botero
authors.affiliation: Instituto de Genetica, Universidad Javeriana, Carrera 7 No 45-02, Bogota, Colombia.
authors.forename: Jaime E
authors.initials: JE
authors.lastname: Bernal
authors.affiliation: Facultad de Medicina, Universidad de La Sabana, Campus Universitario Puente del Común, Km 7 Autopista Norte, Chía, Colombia.
authors.forename: Rosalía
authors.initials: R
authors.lastname: Pérez
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia; Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Santiago, Chile.
authors.forename: George E
authors.initials: GE
authors.lastname: Barreto
authors.affiliation: Facultad de Medicina, Universidad de La Sabana, Campus Universitario Puente del Común, Km 7 Autopista Norte, Chía, Colombia; Grupo Evidencia Terapéutica, Departamento de Farmacología, Universidad de La Sabana, Colombia.
authors.forename: Rosa Helena
authors.initials: RH
authors.lastname: Bustos
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D009457:Neuroglia / Q000648:ultrastructure*; D009830:Olfactory Bulb / Q000648:ultrastructure*; D009832:Olfactory Nerve / Q000648:ultrastructure*; D061251:Primary Cell Culture; D017208:Rats, Wistar
keywords: Cell therapy; Electron microscopy; Neural repair; Olfactory ensheathing cells; Regeneration