title: Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stranded, non-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we demonstrated the down-regulation of miR-148a in HCC and non-cancerous tissues using qRT-PCR.
  
  
  METHODS
  Ninety six HCC samples and their noncancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-148a expression was determined using qRT-PCR. Furthermore, the correlation between the miR-148a expression and clinicopathological parameters was investigated.
  
  
  RESULTS
  The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes. Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC in the current study. Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues.
  
  
  CONCLUSIONS
  Down-regulation of miR-148a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.
authors.affiliation: Gastrointestinal and Liver Disease Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ajdarkosh
authors.affiliation: Department of Pathology, Imam Reza Hospital, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Dadpay
authors.affiliation: Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.affiliation: Department of Biotechnology and Nanotechnology, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Elham Rostami
authors.initials: ER
authors.lastname: Pirzaman
authors.affiliation: Department of Legal Medicine, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Amir Farshid
authors.initials: AF
authors.lastname: Fayyaz
authors.affiliation: Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran.
authors.forename: Ebrahim Khodaverdi
authors.initials: EK
authors.lastname: Darian
authors.affiliation: Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran. a.mokarizadeh@muk.ac.ir.
authors.forename: Aram
authors.initials: A
authors.lastname: Mokarizadeh
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000401:mortality; D011379:Prognosis; D012372:ROC Curve; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C550570:MIRN148 microRNA, human; D035683:MicroRNAs