title: Study of Biological Degradation of New Poly(Ether-Urethane-Urea)s Containing Cyclopeptide Moiety and PEG by Bacillus amyloliquefaciens Isolated from Soil.
abstract: The present work for the first time investigates the effect of Bacillus amyloliquefaciens, M3, on a new poly(ether-urethane-urea) (PEUU). PEUU was synthesized via reaction of 4,4'-methylenebis(4-phenylisocyanate) (MDI), L-leucine anhydride cyclopeptide (LACP) as a degradable monomer and polyethylene glycol with molecular weight of 1000 (PEG-1000). Biodegradation of the synthesized PEUU as the only source for carbon and nitrogen for M3 was studied. The co-metabolism biodegradation of the polymer by this organism was also investigated by adding mannitol or nutrient broth to the basic media. Biodegradation of the synthesized polymer was followed by SEM, FT-IR, TGA, and XRD techniques. It was shown that incubation of PEUU with M3 resulted in a 30-44 % reduction in polymer's weight after 1 month. This study indicates that the chemical structure of PEUU significantly changes after exposure to M3 due to hydrolytic and enzymatic degradation of polymer chains. The results of this work supports the idea that this poly(ether-urethane) is used as a sole carbon source by M3 and this bacterium has a good capability for degradation of poly(ether-urethane)s.
authors.affiliation: Polymer Chemistry Research Laboratory, Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Islamic Republic of Iran. Frafiemanzelat@chem.ui.ac.ir.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Rafiemanzelat
authors.affiliation: Polymer Chemistry Research Laboratory, Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Islamic Republic of Iran.
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Jafari
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, P.O. Box 117, Isfahan, Islamic Republic of Iran. Emtiazi@sci.ui.ac.ir.
authors.forename: Giti
authors.initials: G
authors.lastname: Emtiazi
mesh_terms: D065966:Absorption, Physicochemical; D001407:Bacillus / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D008970:Molecular Weight; D010456:Peptides, Cyclic / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry* / Q000378:metabolism*; D012988:Soil Microbiology; D014867:Water / Q000737:chemistry
chemical_list: D010456:Peptides, Cyclic; D011140:Polyurethanes; C104742:poly(etherurethane urea); D014867:Water; D011092:Polyethylene Glycols
keywords: Amino acid anhydride cyclopeptide; Bacillus amyloliquefaciens; Biodegradation; PEG; Poly(ether-urethane-urea)