title: Building a Synthetic Transcriptional Oscillator.
abstract: Reaction circuits mimicking genetic oscillators can be realized with synthetic, switchable DNA genes (so-called genelets), and two enzymes only, an RNA polymerase and a ribonuclease. The oscillatory behavior of the genelets is driven by the periodic production and degradation of RNA effector molecules. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can be followed in real-time by fluorescence read-out.
authors.affiliation: Systems Biophysics and Bionanotechnology, Physik Department and WSI/ZNN, Technische Universität München, Am Coulombwall 4a, 85748, Garching, Germany.
authors.forename: Matthaeus
authors.initials: M
authors.lastname: Schwarz-Schilling
authors.forename: Jongmin
authors.initials: J
authors.lastname: Kim
authors.forename: Christian
authors.initials: C
authors.lastname: Cuba
authors.forename: Maximilian
authors.initials: M
authors.lastname: Weitz
authors.forename: Elisa
authors.initials: E
authors.lastname: Franco
authors.forename: Friedrich C
authors.initials: FC
authors.lastname: Simmel
mesh_terms: D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D025461:Feedback, Physiological; D005470:Fluorometry; D005813:Genes, Synthetic / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D004247:DNA