title: Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows.
abstract: The mammary gland of dairy cows is a formidable lipid-synthesizing machine for lactation. This unique function depends on the activities of plasma membrane (PM) proteins in mammary cells. Little information is known about the expression profiles of PM proteins and their functions during the lactating process. This study investigated the proteome map of PM fractions of mammary gland in lactating cows using 1D-Gel-LC-MS/MS and identified 872 nonredundant proteins with 141 unknown proteins, wherein 215 were PM-associated proteins. Most of the PM-associated proteins were binding, transport, and catalytic proteins such as annexin proteins, heat shock proteins, integrins, RAS oncogene family members, and S100 calcium binding proteins. The PM-associated pathways such as caveolae-mediated endocytosis, leukocyte extravasation, aldosterone signaling in epithelial cells, and remodeling of epithelial adherens junctions were also significantly over-represented. Proteomic analysis revealed the characteristics and predicted functions of PM proteins isolated from the lactating bovine mammary gland. These results further provide experimental evidence for the presence of many proteins predicted in the annotated bovine genome. The data generated here also provide a reference for the PM-related functional research in the mammary gland of lactating cows.
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Qiongxian
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Shaoxun
authors.initials: S
authors.lastname: Tang
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Han
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Chuanshe
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Jinhe
authors.initials: J
authors.lastname: Kang
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D007774:Lactation / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000145:classification / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D008565:Membrane Proteins; D020543:Proteome
keywords: Nano-LC-ESI-MS/MS; bovine; mammary gland; plasma membrane; proteomics