title: Screening genes associated with myocardial infarction and transverse aortic constriction using a combined analysis of miRNA and mRNA microarray.
abstract: Myocardial infarction (MI) and transverse aortic constriction (TAC) are two models of cardiac hypertrophy. To study mechanisms of MI and TAC, GSE415 and GSE14267 were downloaded from Gene Expression Omnibus. GSE415 included left ventricle (LV) and intraventricular septum samples from mice that underwent MI, TAC or sham operation. GSE14267 included normal and MI samples from non-transgenic mice. Differentially expressed genes (DEGs) and microRNAs (miRNAs) were screened using limma package. Functional enrichment analysis was performed for DEGs using DAVID. Common DEGs of different groups were conducted for protein-protein interaction (PPI) analysis using STRING and visualized in PPI network by Cytoscape. Furthermore, targets were predicted for differentially expressed miRNAs using TarMir database. Totally, 277 DEGs, 31 common DEGs (e.g. SFRP2), 6 differentially expressed miRNAs (e.g. mmu-miR-448) and 1 miRNA-mRNA pair (mmu-miR-448→SIM2) were screened out. DEGs were significantly enriched in biological processes related to muscle development and ion transportation. In the PPI network for common DEGs, LOX (degree=7), POSTN (degree=5), SPARC (degree=4) and TIMP1 (degree=3) were with higher degrees. In addition, they might function by interacting with each other (e.g. LOX-TIMP1, LOX-POSTN, SPARC-TIMP1 and SPARC-POSTN). In conclusion, LOX, POSTN, SPARC, TIMP1 and SFRP2 might affect MI and TAC.3.
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wan
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Emergency and Intensive Care Unit, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: zhcheye@163.com.
authors.forename: Chengye
authors.initials: C
authors.lastname: Zhan
mesh_terms: D000818:Animals; D001024:Aortic Valve Stenosis / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D056726:Genetic Association Studies; D005820:Genetic Testing; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods; D009203:Myocardial Infarction / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Differentially expressed gene; Differentially expressed microRNA; Myocardial infarction; Protein-protein interaction network; Transverse aortic constriction