title: Enzymatic synthesis of 2-deoxyglucose-containing maltooligosaccharides for tracing the location of glucose absorption from starch digestion.
abstract: The ileal brake mechanism which induces a potentially beneficial slower gastric emptying rate and increased satiety is triggered by macronutrients including glucose from glycemic carbohydrates. For optimization of this diet-induced health benefit, there is the need for a way to determine the location of glucose deposition in the small intestine. Labeled 2-deoxyglucose (2-DG) can be used to trace the location of glucose absorption due to its accumulative property in the small intestine enterocytes. However, because pure glucose, or 2-DG, is directly absorbed in the proximal small intestine, we designed 2-DG containing maltooligosaccharides (2-DG-MOs) that can be used with a mild α-glucosidase inhibitor to attain an analytical method for determining location-specific delivery of glucose and its physiological effect.
authors.affiliation: Department of Food Science & Biotechnology, College of BioNano Technology, Gachon University, Seongnam, Republic of Korea.
authors.forename: Byung-Hoo
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Food Science & Technology, BK21+ Project Team, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, Republic of Korea.
authors.forename: Dong-Wan
authors.initials: DW
authors.lastname: Koh
authors.affiliation: Department of Radiology and Imaging Sciences, Indiana University, School of Medicine, Indianapolis, IN, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Territo
authors.affiliation: Graduate School of Biotechnology, and Institute of Life Science and Resources, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
authors.affiliation: Whistler Center for Carbohydrate Research and Department of Food Science, Purdue University, West Lafayette, IN, USA. Electronic address: hamakerb@purdue.edu.
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Hamaker
authors.affiliation: Department of Food Science & Technology, BK21+ Project Team, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, Republic of Korea. Electronic address: shyoo@sejong.ac.kr.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Yoo
mesh_terms: D000818:Animals; D003847:Deoxyglucose / Q000032:analysis / Q000378:metabolism*; D004063:Digestion; D005947:Glucose / Q000032:analysis* / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007082:Ileum / Q000502:physiology*; D009343:Neisseria / Q000201:enzymology; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D009844:Oligosaccharides; C021705:maltooligosaccharides; D013213:Starch; D003847:Deoxyglucose; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D005947:Glucose
keywords: 2-Deoxyglucose (2-DG); Amylosucrase; Glucose absorption; Maltooligosaccharides; Mucosal α-glucosidases