title: The Marine-Derived Fungus Clonostachys rosea, Source of a Rare Conjugated 4-Me-6E,8E-hexadecadienoic Acid Reducing Viability of MCF-7 Breast Cancer Cells and Gene Expression of Lipogenic Enzymes.
abstract: A marine-derived strain of Clonostachys rosea isolated from sediments of the river Loire estuary (France) was investigated for its high lipid production. The fungal strain was grown on six different culture media to explore lipid production changes. An original branched conjugated fatty acid, mainly present in triglycerides and mostly produced when grown on DCA (23% of total fatty acid composition). It was identified as 4-Me-6E,8E-hexadecadienoic on the basis of spectroscopic analyses. This fatty acid reduced viability of MCF-7 breast cancer cells in a dose dependent manner (up to 63%) at physiological free fatty acid human plasma concentration (100 μM). Reduction of gene expression of two lipogenic enzymes, the acetyl CoA carboxylase (ACC) and the fatty acid synthase (FAS) was evaluated to explore the mechanisms of action of 4-Me-6E,8E-16:2 acid. At 50 μM, 50% and 35% of mRNA gene expression inhibition were observed for ACC and FAS, respectively.
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. acamiladias@gmail.com.
authors.forename: Ana Camila Dos Santos
authors.initials: AC
authors.lastname: Dias
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. nicolas.ruiz@univ-nantes.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ruiz
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Couzinet-Mossion
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. samuel.bertrand@univ-nantes.fr.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bertrand
authors.affiliation: Faculty of Pharmacy, University of Nantes, IICiMed, 9 Rue Bias, 44000 Nantes, France. muriel.duflos@univ-nantes.fr.
authors.forename: Muriel
authors.initials: M
authors.lastname: Duflos
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. yves-francois.pouchus@univ-nantes.fr.
authors.forename: Yves-François
authors.initials: YF
authors.lastname: Pouchus
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. gilles.barnathan@univ-nantes.fr.
authors.forename: Gilles
authors.initials: G
authors.lastname: Barnathan
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. el-hassane.nazih@univ-nantes.fr.
authors.forename: Hassan
authors.initials: H
authors.lastname: Nazih
authors.affiliation: Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. gaetane.wielgosz-collin@univ-nantes.fr.
authors.forename: Gaetane
authors.initials: G
authors.lastname: Wielgosz-Collin
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D059001:Aquatic Organisms / Q000378:metabolism*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects* / Q000235:genetics; D064429:Fatty Acid Synthases / Q000235:genetics; D005227:Fatty Acids / Q000235:genetics; D005260:Female; D005602:France; D005658:Fungi / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D061986:MCF-7 Cells; D012333:RNA, Messenger / Q000235:genetics; D014280:Triglycerides / Q000235:genetics
chemical_list: D005227:Fatty Acids; D012333:RNA, Messenger; D014280:Triglycerides; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase
keywords: Clonostachys rosea; MCF-7; acetyl-CoA carboxylase; anti-carcinogenic; cancer; conjugated fatty acid; fatty acid synthase; fatty acids; lipids; lipogenesis