title: Antiviral and Antioxidant Activities of Sulfated Galactomannans from Plants of Caatinga Biome.
abstract: Dengue represents a serious social and economic public health problem; then trying to contribute to improve its control, the objective of this research was to develop phytoterapics for dengue treatment using natural resources from Caatinga biome. Galactomannans isolated from Adenanthera pavonina L., Caesalpinia ferrea Mart., and Dimorphandra gardneriana Tull were chemically sulfated in order to evaluate the antioxidant, and antiviral activities and the role in the inhibition of virus DENV-2 in Vero cells. A positive correlation between the degree of sulfation, antioxidant and antiviral activities was observed. The sulfated galactomannans showed binding to the virus surface, indicating that they interact with DENV-2. The sulfated galactomannans from C. ferrea showed 96% inhibition of replication of DENV-2 followed by D. gardneriana (94%) and A. pavonina (77%) at 25 µg/mL and all sulfated galactomannans also showed antioxidant activity. This work is the first report of the antioxidant and antiviral effects of sulfated galactomannans against DENV-2. The results are very promising and suggest that these sulfated galactomannans from plants of Caatinga biome act in the early step of viral infection. Thus, sulfated galactomannans may act as an entry inhibitor of DENV-2.
authors.affiliation: Postgraduate Program of Biotechnology, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil.
authors.forename: Márcia Maria Mendes
authors.initials: MM
authors.lastname: Marques
authors.affiliation: Postgraduate Program of Biotechnology, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil ; Course of Chemistry, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil.
authors.forename: Selene Maia
authors.initials: SM
authors.lastname: de Morais
authors.affiliation: Postgraduate Program of Biotechnology, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil.
authors.forename: Ana Raquel Araújo
authors.initials: AR
authors.lastname: da Silva
authors.affiliation: Course of Chemistry, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil.
authors.forename: Naiara Dutra
authors.initials: ND
authors.lastname: Barroso
authors.affiliation: Postgraduate Program of Biotechnology, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil.
authors.forename: Tadeu Rocha
authors.initials: TR
authors.lastname: Pontes Filho
authors.affiliation: Public Health Central Laboratory of Ceará, Avenue Barão de Sturdat 2405, 60120-002 Fortaleza, CE, Brazil.
authors.forename: Fernanda Montenegro de Carvalho
authors.initials: FM
authors.lastname: Araújo
authors.affiliation: Technological Development Park (PADETEC), Federal University of Ceará, Campus do Pici, 60455-970 Fortaleza, CE, Brazil.
authors.forename: Ícaro Gusmão Pinto
authors.initials: ÍG
authors.lastname: Vieira
authors.affiliation: Health Sciences Center, University of Fortaleza, Avenue Washington Soares 1321, 60811-905 Fortaleza, CE, Brazil.
authors.forename: Danielle Malta
authors.initials: DM
authors.lastname: Lima
authors.affiliation: Postgraduate Program of Biotechnology, Ceará State University, Avenue Dr. Silas Munguba 1700, Campus do Itaperi, 60714-903 Fortaleza, CE, Brazil.
authors.forename: Maria Izabel Florindo
authors.initials: MI
authors.lastname: Guedes