title: ErbB3 binding protein 1 (EBP1) participates in the regulation of intestinal inflammation via mediating Akt signaling pathway.
abstract: ErbB3 binding protein-1 (EBP1) belongs to a family of DNA/RNA binding proteins implicated in cell growth, differentiation and apoptosis. Previous data demonstrated that EBP1 regulates phosphorylation of Akt to drive tumor progress. However, the expression and biological functions of EBP1 in ulcerative colitis (UC) remain unclear. In this study, we reported for the first time that EBP1 was down-regulated in intestinal epithelial cell (IECs) of patients with UC. In DSS-induced colitis, we observed the down-regulation of EBP1 accompanied with the elevated levels of proinflammatory cytokines (IL-1β, IL-6 and IL-8) and Akt activation indicators (phosphorylated Akt) in colitis IECs, indicating the possible involvement of EBP1 in regulation of intestinal inflammation via mediating Akt in UC. Employing the TNF-α-treated HT-29 cells as an IEC inflammatory model, we confirmed the negative correlation of EBP1 with Akt activation and Akt-dependent inflammation progress in vitro. EBP1 knocking down and over-expression significantly regulated TNF-α-induced Akt activation and proinflammatory cytokines expression in HT-29 cells. Taken together, our data suggested that EBP1 participates in the regulation of intestinal inflammation via mediating Akt signaling pathway.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Xianjing
authors.initials: X
authors.lastname: Miao
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210000, Jiangsu Province, People's Republic of China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Pathology, Affiliated Cancer Hospital of Nantong University, 30 North Tongyang Road, Pingchao, Nantong 226361, Jiangsu Province, People's Republic of China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Miao
authors.affiliation: Department of Pathology, Affiliated Cancer Hospital of Nantong University, 30 North Tongyang Road, Pingchao, Nantong 226361, Jiangsu Province, People's Republic of China.
authors.forename: Yaxun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 20 Xisi Road, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Liren
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu Province, People's Republic of China. Electronic address: zdm@ntu.edu.cn.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D015536:Down-Regulation / Q000187:drug effects; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D019073:HT29 Cells; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology*; D007422:Intestines / Q000473:pathology*; D008810:Mice, Inbred C57BL; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C101633:PA2G4 protein, human; D016601:RNA-Binding Proteins; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt signaling pathway; ErbB3 binding protein-1 (EBP1); Inflammation; Intestinal epithelial cell (IEC); TNF-α; Ulcerative colitis (UC)