title: Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film.
abstract: A highly sensitive electrochemical biosensor for the detection of Bisphenol A (BPA) in water has been developed by immobilizing tyrosinase onto a diazonium-functionalized boron doped diamond electrode (BDD) modified with multi-walled carbon nanotubes (MWCNTs). The fabricated biosensor exhibits excellent electroactivity towards o-quinone, a product of this enzymatic reaction of BPA oxidation catalyzed by tyrosinase. The developed BPA biosensor displays a large linear range from 0.01 nM to 100 nM, with a detection limit (LOD) of 10 pM. The feasibility of the proposed biosensor has been demonstrated on BPA spiked water river samples. Therefore, it could be a promising and reliable analytical tool for on-site monitoring of BPA in waste water.
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France; University of Annaba, Faculty of Engineering Sciences, Laboratory of Environmental Engineering, B.P.12, Annaba 23000, Algeria.
authors.forename: Nedjla
authors.initials: N
authors.lastname: Zehani
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Fortgang
authors.affiliation: University of Annaba, Faculty of Engineering Sciences, Laboratory of Environmental Engineering, B.P.12, Annaba 23000, Algeria.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Saddek Lachgar
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France.
authors.forename: Abdoullatif
authors.initials: A
authors.lastname: Baraket
authors.affiliation: University of Sud ToulonVar - Institute of Materials Microelectronics and Nanosciences of Provence, UMR CNRS 7334, La Garde, France.
authors.forename: Madjid
authors.initials: M
authors.lastname: Arab
authors.affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, Kiev 03143, Ukraine.
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Dzyadevych
authors.affiliation: University of Annaba, Faculty of Engineering Sciences, Laboratory of Environmental Engineering, B.P.12, Annaba 23000, Algeria.
authors.forename: Rochdi
authors.initials: R
authors.lastname: Kherrat
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D001895:Boron / Q000737:chemistry; D003979:Diazonium Compounds; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D057230:Limit of Detection; D014442:Monophenol Monooxygenase / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D062065:Wastewater / Q000737:chemistry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D001895:Boron; D003979:Diazonium Compounds; D004800:Enzymes, Immobilized; D014442:Monophenol Monooxygenase; D037742:Nanotubes, Carbon; D010636:Phenols; D062065:Wastewater; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Biosensor; Bisphenol A; Diamond electrode; Diazonium; Tyrosinase