title: Functional genetic variants in pre-miR-146a and 196a2 genes are associated with risk of lung cancer in North Indians.
abstract: OBJECTIVE
  To find the association of two pre-miRNA polymorphisms with risk of lung cancer in North Indians.
  
  
  METHODS
  Genotyping of 250 cases and 255 controls for miR-146a and miR-196a2 using PCR-RFLP.
  
  
  RESULTS
  Heterozygous subjects showed a risk toward lung cancer (LC), especially for adenocarcinoma (OR: 1.82; 95% CI: 1.04-3.20; p = 0.03) in miR-146a gene. TT genotype for miR-196a2 gene also showed 3.2-fold risk toward LC and the risk was fivefold higher for squamous cell carcinoma. Survival rate was significantly lower in subjects with TT genotype as compared with the CC genotype in miR-196a2.
  
  
  CONCLUSIONS
  Both the single nucleotide polymorphism variants showed a positive association toward risk of lung cancer.
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, Punjab 147002, India.
authors.forename: Kushaldeep Kaur
authors.initials: KK
authors.lastname: Sodhi
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, Punjab 147002, India.
authors.forename: Charu
authors.initials: C
authors.lastname: Bahl
authors.affiliation: Department of Pulmonary Medicine, Post Graduate Institute of Education & Medical Research (PGIMER), Sector 14, Chandigarh, India.
authors.forename: Navneet
authors.initials: N
authors.lastname: Singh
authors.affiliation: Department of Pulmonary Medicine, Post Graduate Institute of Education & Medical Research (PGIMER), Sector 14, Chandigarh, India.
authors.forename: Digamber
authors.initials: D
authors.lastname: Behera
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, Punjab 147002, India.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Sharma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: genotype; lung cancer; miR-146a; miR-196a2; single nucleotide polymorphism