title: Genotypic Diversity of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from Inpatients and Outpatients.
abstract: We investigated the prevalence of methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolated from hospitalized patients and outpatients (OP). Out of 350 staphylococcal isolates collected from three hospitals, 190 were coagulase-negative staphylococci (CoNS). These isolates were subjected to antimicrobial susceptibility tests, detection of mecA, and pulsed-field gel electrophoresis (PFGE) typing. Among the 190 isolated CoNS, Staphylococcus epidermidis (47.3%) and Staphylococcus haemolyticus (44.2%) were the most prevalent species. Other CoNS species that were isolated were Staphylococcus saprophyticus (2.1%), Staphylococcus warneri (2.1%), Staphylococcus simulans (1.6%), Staphylococcus capitis (1.1%), Staphylococcus schleiferi (1.1%), and Staphylococcus hominis (0.5%). The rate of resistance to methicillin was 60% with 58 (50%) S. epidermidis and 55 (49%) S. haemolyticus. The rate of resistance to 13 antibiotics tested with the lowest and highest to chloramphenicol and penicillin, respectively. High clonal diversity with different PFGE patterns was obtained for methicillin-resistant S. epidermidis and S. haemolyticus by 32 and 31 types, respectively. Our results indicated that the dissemination of MRCoNS is widespread in Tehran. The majority of these isolates showed distinct genotyping patterns. At the same time, the common patterns were found among the MRCoNS obtained from outpatient and inpatient isolates, suggestive of an epidemiological link.
authors.affiliation: 1 Department of Microbiology, School of Medicine, Iran University of Medical Sciences , Tehran, Iran .
authors.forename: Malihe
authors.initials: M
authors.lastname: Talebi
authors.affiliation: 2 Department of Microbiology, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shafiee
authors.affiliation: 3 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Javad
authors.initials: J
authors.lastname: Sadeghi
authors.affiliation: 2 Department of Microbiology, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Nasrin Asghari
authors.initials: NA
authors.lastname: Moghadam
authors.affiliation: 4 Department of Mycobacteriology, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Saifi
authors.affiliation: 2 Department of Microbiology, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Pourshafie
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D002999:Clone Cells; D003030:Coagulase / Q000172:deficiency / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007297:Inpatients; D007492:Iran; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D010045:Outpatients; D010406:Penicillins / Q000494:pharmacology; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D041162:Staphylococcus haemolyticus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D041181:Staphylococcus hominis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003030:Coagulase; C487495:MecA protein, Staphylococcus epidermidis; D010406:Penicillins; D002701:Chloramphenicol; D008712:Methicillin