title: Correlation between mesopore volume of carbon supports and the immobilization of laccase from Trametes versicolor for the decolorization of Acid Orange 7.
abstract: Immobilization of laccase from Trametes versicolor was carried out using carbon supports prepared from different lignocellulosic wastes. Enzymes were immobilized by physical adsorption. Taguchi methodology was selected for the design of experiments regarding the preparation of the carbon materials, which included the use of activating agents for the promotion of mesoporosity. A good correlation between the mesopore volumes of the carbon supports and the corresponding laccase loadings attained was observed. Specifically, the chemical activation of pecan nut shell with FeCl3 led to a highly mesoporous material that also behaved as the most efficient support for the immobilization of laccase. This particular laccase/carbon support system was used as biocatalyst for the decolorization of aqueous solutions containing Acid Orange 7. Mass spectrometry coupled to a liquid chromatograph allowed us to identify the products of the dye degradation.
authors.affiliation: Instituto Tecnológico de Aguascalientes, Av. Adolfo López Mateos No. 1801 Ote., C.P. 20256, Aguascalientes, Ags., Mexico.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Ramírez-Montoya
authors.affiliation: Instituto Tecnológico de Aguascalientes, Av. Adolfo López Mateos No. 1801 Ote., C.P. 20256, Aguascalientes, Ags., Mexico. Electronic address: virginia.hernandez@yahoo.com.mx.
authors.forename: Virginia
authors.initials: V
authors.lastname: Hernández-Montoya
authors.affiliation: Instituto Nacional del Carbón, INCAR-CSIC, Apartado 73, E-33080, Oviedo, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Montes-Morán
authors.affiliation: División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4ª, Sección, San Luis Potosí, SLP, 78216 Mexico.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Cervantes
mesh_terms: D000327:Adsorption; D000363:Agaricales / Q000378:metabolism; D001391:Azo Compounds / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D002244:Carbon / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D055454:Trametes / Q000201:enzymology*; D018508:Water Purification / Q000379:methods*
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D002244:Carbon; D042845:Laccase; C014722:2-naphthol orange
keywords: Acid Orange 7; Degradation; Immobilization; Laccase; Trametes versicolor