title: Nodulisporisteroids C-L, new 4-methyl-progesteroid derivatives from Nodulisporium sp.
abstract: The fungus Nodulisporium sp. (No. 65-12-7-1), which can produce a rare class of steroids (4-methyl-progesteroids) in rice solid medium, was subjected to a one strain-many compounds (OSMAC) approach. It was found to produce ten new 4-methyl-progesteroid derivatives named nodulisporisteroids C-L (1-10) in potato-dextrose-broth (PDB) medium, which showed that the fungus was a prolific producer of 4-methyl-progesteroids. The structures of 1-10 were elucidated by spectroscopic and X-ray crystallographic analysis. Their cytotoxic activities against five human cancer cell lines had been evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Gao-Qian
authors.initials: GQ
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address: chgdtong@163.com.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Chen
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Hu
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Liang-Dong
authors.initials: LD
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Photochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address: tghao@jnu.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Gao
mesh_terms: D006801:Humans; D015394:Molecular Structure; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification*; D013098:Xylariales / Q000737:chemistry*
chemical_list: D013256:Steroids
keywords: 4-Methyl-progesteroid derivatives; Endolichenic fungus; Nodulisporium sp.; Xylariaceae