title: Characterization of the complete mitochondrial genome of the red crayfish, Procambarus clarkii (Decapoda: Cambaridae).
abstract: Here we present the complete mitochondrial (mt) genome of Procambarus clarkii (Decapoda: Cambaridae) and provide its annotation. The complete mt genome was determined to be 15 929 bp and contains 22 tRNA genes, 13 protein-coding genes (PCGs), two rRNA genes, and a D-loop region. The nucleotide composition was biased toward A + T nucleotides (72.91%) and the AT skew of this mt genome was slightly negative. All the 22 tRNA genes displayed a typical clover-leaf structure, with the exception of trnS1 (AGN). About 13 PCGs were initiated by ATN codons, except for cox1 and nad2 genes which were initiated by ACG and GTG, respectively. Six of the 13 PCGs harbor the incomplete termination codon by T or TA. The D-loop region of the mt genome was 1188 bp in length and the A + T content was 81.08%. Phylogenetic analysis showed that the placement of P. clarkii was within the Cambaridae. This mt genome sequence will provide a better understanding for crayfish evolution in the future.
authors.affiliation: a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University , Yancheng , PR China.
authors.forename: Qiu-Ning
authors.initials: QN
authors.lastname: Liu
authors.affiliation: a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University , Yancheng , PR China.
authors.forename: Xin-Yue
authors.initials: XY
authors.lastname: Chai
authors.affiliation: a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University , Yancheng , PR China.
authors.forename: Sen-Hao
authors.initials: SH
authors.lastname: Jiang
authors.affiliation: a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University , Yancheng , PR China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Zhou
authors.affiliation: a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University , Yancheng , PR China.
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Xuan
authors.affiliation: a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University , Yancheng , PR China.
authors.forename: Bo-Ping
authors.initials: BP
authors.lastname: Tang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000235:genetics*; D001482:Base Composition; D004275:DNA, Ribosomal / Q000235:genetics; D023061:Gene Order; D059646:Genome Size; D054629:Genome, Mitochondrial; D008928:Mitochondria / Q000235:genetics*; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004275:DNA, Ribosomal; D012343:RNA, Transfer
keywords: Cambaridae; Decapoda; Procambarus clarkii; mitochondrial genome