title: More signs of neurotoxicity of surfactants and flame retardants - Neonatal PFOS and PBDE 99 cause transcriptional alterations in cholinergic genes in the mouse CNS.
abstract: Maternally and lactionally transferred persistent organic pollutants may interfere with CNS development. Here, 10-day-old male mice were exposed to single oral doses of PFOS (perflourooctanosulphonate) or PBDE 99 (2,2',4,4',5-penta-bromodiphenyl ether), and examined for changes in cholinergic gene transcription in the CNS 24h and 7 weeks later. 24h after exposure qPCR analyses revealed decreased transcription of nAChR-β2 and AChE in cortex, and increased mAChR-5 in hippocampus of PFOS treated mice. Neonatal PFOS treatment altered spontaneous behaviour at 2 months of age but did not affect gene transcription in adults. At 2 months of age neonatally PBDE 99 treated mice had altered spontaneous behaviour, and cortical transcription of AChE, nAChR-α4, nAChR-β2 and mAChR-5 were elevated. Our results indicate that PFOS and PBDE 99 affects the developing central cholinergic system by altering gene transcription in cortex and hippocampus, which may in part account for mechanisms causing changes in spontaneous behaviour.
authors.affiliation: Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden. Electronic address: stefan.hallgren@ebc.uu.se.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hallgren
authors.affiliation: Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Fredriksson
authors.affiliation: Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Viberg
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics; D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000254:growth & development; D005411:Flame Retardants / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D058851:GPI-Linked Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008297:Male; D051379:Mice; D043642:Receptor, Muscarinic M5 / Q000235:genetics; D011978:Receptors, Nicotinic / Q000235:genetics
chemical_list: D000110:Acetylcholinesterase; D017738:Alkanesulfonic Acids; D005411:Flame Retardants; D005466:Fluorocarbons; D058851:GPI-Linked Proteins; D055768:Halogenated Diphenyl Ethers; D043642:Receptor, Muscarinic M5; D011978:Receptors, Nicotinic; C086401:pentabromodiphenyl ether; C076994:perfluorooctane sulfonic acid; C548245:Ache protein, mouse
keywords: Brain growth spurt; Brominated flame retardants; Cholinergic system; Developmental neurotoxicology; Perflourinated chemical repellants