title: Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool.
abstract: Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For a large mass-casualty event involving exposure to ionizing radiation, it is of utmost importance to rapidly provide dose information for medical treatment. The well-established cytokinesis-block micronucleus (CBMN) assay is a validated method for biodosimetry. However, the need for an accelerated sample processing is required for the CBMN assay to be a suitable population triage tool. We report here on the development of a high-throughput and miniaturized version of the CMBN assay for accelerated sample processing.
authors.affiliation: a  Center for Radiological Research, Department of Radiation Oncology, Columbia University Medical Center, New York, New York 10032; and.
authors.forename: Stanley W
authors.initials: SW
authors.lastname: Lue
authors.affiliation: a  Center for Radiological Research, Department of Radiation Oncology, Columbia University Medical Center, New York, New York 10032; and.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Repin
authors.affiliation: b  Northrop Grumman, Elkridge, Maryland 21075.
authors.forename: Ryan
authors.initials: R
authors.lastname: Mahnke
authors.affiliation: a  Center for Radiological Research, Department of Radiation Oncology, Columbia University Medical Center, New York, New York 10032; and.
authors.forename: David J
authors.initials: DJ
authors.lastname: Brenner
mesh_terms: D048749:Cytokinesis / Q000528:radiation effects; D057166:High-Throughput Screening Assays; D006801:Humans; D015162:Micronucleus Tests; D011839:Radiation, Ionizing; D011874:Radiometry / Q000295:instrumentation / Q000379:methods*; D014218:Triage