title: Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation.
abstract: Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of targeted, protective polymer coatings on cells for the high speed enrichment of cells. Individual, antigen-positive cells are coated with a biocompatible hydrogel which protects the cells from a surfactant solution, while uncoated cells are immediately lysed. After lysis, the polymer coating is removed through orthogonal photochemistry, and the isolate has >50% yield of viable cells and these cells proliferate at rates comparable to control cells. Minority cell populations are enriched from erythrocyte-depleted blood to >99% purity, whereas the entire batch process requires 1 h and <$2000 in equipment. Batch scale-up is only contingent on irradiation area for the coating photopolymerization, as surfactant-based lysis can be easily achieved on any scale.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Romero
authors.forename: Jacob J
authors.initials: JJ
authors.lastname: Lilly
authors.affiliation: §Department of Chemical Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.
authors.forename: Nathan S
authors.initials: NS
authors.lastname: Abraham
authors.forename: Hainsworth Y
authors.initials: HY
authors.lastname: Shin
authors.affiliation: ||Department of Pediatrics, University of Wisconsin, Madison, Wisconsin 53792, United States.
authors.forename: Vivek
authors.initials: V
authors.lastname: Balasubramaniam
authors.affiliation: #Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536, United States.
authors.forename: Tadahide
authors.initials: T
authors.lastname: Izumi
authors.forename: Brad J
authors.initials: BJ
authors.lastname: Berron
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D019169:Jurkat Cells; D017493:Leukocyte Common Antigens / Q000276:immunology; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000951:Antigens, Neoplasm; D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D020100:Hydrogels; D011108:Polymers; D013501:Surface-Active Agents; D017493:Leukocyte Common Antigens
keywords: cell isolation; coatings; photopolymerization; polymer; protein expression; sorting