title: Salmonella Typhimurium strain expressing OprF-OprI protects mice against fatal infection by Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa poses a major threat to human health and to the mink industry. Thus, development of vaccines that elicit robust humoral and cellular immunity against P. aeruginosa is greatly needed. In this study, a recombinant attenuated Salmonella vaccine (RASV) that expresses the outer membrane proteins fusion OprF190-342 -OprI21-83 (F1I2) from P. aeruginosa was constructed and the potency of this vaccine candidate assessed by measuring F1I2-specific humoral immune responses upon vaccination through s.c. or oral routes. S.C. administration achieved higher serum IgG titers and IgA titers in the intestine and induced stronger F1I2-specific IgG and IgA titers in lung homogenate than did oral administration, which resulted in low IgG titers and no local IgA production. High titers of IFN-γ, IL-4, and T-lymphocyte subsets induced a mixed Th1/Th2 response in mice immunized s.c., indicating elicitation of cellular immunity. Importantly, when immunized mice were challenged with P. aeruginosa by the intranasal route 30 days after the initial immunization, s.c. vaccination achieved 77.78% protection, in contrast to 41.18% via oral administration and 66.67% via Escherichia coli-expressed F1I2 (His-F1I2) vaccination. These results indicate that s.c. vaccination provides a better protective response against P. aeruginosa infection than do oral administration and the His-F1I2 vaccine.
authors.affiliation: College of Veterinary Medicine.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Gu
authors.affiliation: College of Animal Science, Jilin University, No. 5333, Xi'an Street, Changchun, Jilin, 130062.
authors.forename: Xinwu
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine.
authors.forename: Ziyin
authors.initials: Z
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Tong
authors.affiliation: College of Veterinary Medicine.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Veterinary Medicine.|Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009, People's Republic of China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004337:Drug Carriers; D004926:Escherichia coli; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000276:immunology; D008807:Mice, Inbred BALB C; D008907:Mink; D011552:Pseudomonas Infections / Q000517:prevention & control*; D054406:Pseudomonas Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development; D044967:Serum / Q000276:immunology; D016019:Survival Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D016896:Treatment Outcome; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004337:Drug Carriers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008074:Lipoproteins; C547605:OprF protein, Pseudomonas aeruginosa; D054406:Pseudomonas Vaccines; D011994:Recombinant Proteins; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; C060507:outer membrane lipoprotein I, Bacteria; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Pseudomonas aeruginosa; Salmonella Typhimurium; outer membrane protein; subcutaneous vaccination