title: Antitumor and immunomodulatory activity of polysaccharide isolated from Trametes orientalis.
abstract: A water-soluble polysaccharide (TOP-2) was isolated from Trametes orientalis, consisting of galactose, glucose, mannose, and arabinose with the molar ratios of 5.79:5.77:3.45:1, having an average molecular weight of 63kDa. The antitumor and immunomodulatory activity of TOP-2 were determined in Lewis lung carcinoma (LLC) tumor-bearing mice. The results revealed that TOP-2 not only could efficaciously restrain the growth of LLC in mice, but also effectively increase the body weight and relative spleen/thymus weight. In addition, TOP-2 remarkably enhanced splenocyte proliferation, notably stimulated phagocytotic function of macrophages, and strikingly promoted the expression of serum cytokines. These findings indicate that TOP-2 exert antitumor activity in vivo potentially by improving immune function. TOP-2 could be empoldered as a potential supplementary agent for cancer treatment.
authors.affiliation: College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China; Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology, Xuzhou 221000, China. Electronic address: biozheng@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China; Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology, Xuzhou 221000, China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Wang
authors.affiliation: College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China; Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology, Xuzhou 221000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D018827:Carcinoma, Lewis Lung / Q000097:blood / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D002850:Chromatography, Gel; D016207:Cytokines / Q000097:blood; D007155:Immunologic Factors / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000166:cytology; D055454:Trametes / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides
keywords: Antitumor; Characterization; Immunomodulatory; Polysaccharide; Trametes orientalis