title: Carbon nanotubes supported tyrosinase in the synthesis of lipophilic hydroxytyrosol and dihydrocaffeoyl catechols with antiviral activity against DNA and RNA viruses.
abstract: Hydroxytyrosol and dihydrocaffeoyl catechols with lipophilic properties have been synthesized in high yield using tyrosinase immobilized on multi-walled carbon nanotubes by the Layer-by-Layer technique. All synthesized catechols were evaluated against a large panel of DNA and RNA viruses, including Poliovirus type 1, Echovirus type 9, Herpes simplex virus type 1 (HSV-1), Herpes simplex virus type 2 (HSV-2), Coxsackievirus type B3 (Cox B3), Adenovirus type 2 and type 5 and Cytomegalovirus (CMV). A significant antiviral activity was observed in the inhibition of HSV-1, HSV-2, Cox B3 and CMV. The mechanism of action of the most active dihydrocaffeoyl derivative was investigated against a model of HSV-1 infection.
authors.affiliation: Department of Ecology and Biology, University of Tuscia, Largo dell'Università, 01100 Viterbo (VT), Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Botta
authors.affiliation: Department of Ecology and Biology, University of Tuscia, Largo dell'Università, 01100 Viterbo (VT), Italy.
authors.forename: Bruno Mattia
authors.initials: BM
authors.lastname: Bizzarri
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Microbiological Section, University of Catania (CT), Via Androne, 81 95124 Catania, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Garozzo
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Microbiological Section, University of Catania (CT), Via Androne, 81 95124 Catania, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Timpanaro
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Microbiological Section, University of Catania (CT), Via Androne, 81 95124 Catania, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Bisignano
authors.affiliation: Department of Public Health and Infectious Diseases, 'Sapienza' University, 00185 Rome, Italy; IRCCS San Raffaele Pisana, Telematic University, 00166 Rome, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Amatore
authors.affiliation: Department of Public Health and Infectious Diseases, 'Sapienza' University, 00185 Rome, Italy; IRCCS San Raffaele Pisana, Telematic University, 00166 Rome, Italy.
authors.forename: Anna Teresa
authors.initials: AT
authors.lastname: Palamara
authors.affiliation: Department of Public Health and Infectious Diseases, 'Sapienza' University, 00185 Rome, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Nencioni
authors.affiliation: Department of Ecology and Biology, University of Tuscia, Largo dell'Università, 01100 Viterbo (VT), Italy. Electronic address: saladino@unitus.it.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Saladino
mesh_terms: D000364:Agaricus / Q000201:enzymology; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology*; D002396:Catechols / Q000737:chemistry* / Q000494:pharmacology*; D004266:DNA Virus Infections / Q000188:drug therapy; D004267:DNA Viruses / Q000187:drug effects*; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D012327:RNA Virus Infections / Q000188:drug therapy; D012328:RNA Viruses / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D002396:Catechols; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; C005975:3,4-dihydroxyphenylethanol; D014442:Monophenol Monooxygenase; D010626:Phenylethyl Alcohol
keywords: Antiviral activity; Catechols; DNA and RNA viruses; Dihydrocaffeoyl derivatives; Hydroxytyrosol derivatives