title: A proteomic perspective of the interplay of Staphylococcus aureus and human alveolar epithelial cells during infection.
abstract: Infectious diseases caused by pathogens such as Staphylococcus aureus are still a major threat for human health. Proteome analyses allow detailed monitoring of the molecular interplay between pathogen and host upon internalization. However, the investigation of the responses of both partners is complicated by the large excess of host cell proteins compared to bacterial proteins as well as by the fact that only a fraction of host cells are infected. In the present study we infected human alveolar epithelial A549 cells with S. aureus HG001 pMV158GFP and separated intact bacteria from host cell debris or infected from non-infected A549 cells by cell sorting to enable detailed proteome analysis. During the first 6.5h in the intracellular milieu S. aureus displayed reduced growth rate, induction of the stringent response, adaptation to microaerobic conditions as well as cell wall stress. Interestingly, both truly infected host cells and those not infected but exposed to secreted S. aureus proteins and host cell factors showed differences in the proteome pattern compared to A549 cells which had never been in contact with S. aureus. However, adaptation reactions were more pronounced in infected compared to non-infected A549 bystander cells.
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Surmann
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Simon
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Hildebrandt
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Henrike
authors.initials: H
authors.lastname: Pförtner
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Michalik
authors.affiliation: Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Sauerbruchstr. DZ7, 17475 Greifswald, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Stentzel
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Leif
authors.initials: L
authors.lastname: Steil
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Vishnu M
authors.initials: VM
authors.lastname: Dhople
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Str. 15, 17487 Greifswald, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Bernhardt
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Str. 15, 17487 Greifswald, Germany.
authors.forename: Rabea
authors.initials: R
authors.lastname: Schlüter
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Maren
authors.initials: M
authors.lastname: Depke
authors.affiliation: Institute of Biochemistry, Ernst-Moritz-Arndt-University Greifswald, Felix-Hausdorff-Straße 4, 17487 Greifswald, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Gierok
authors.affiliation: Institute of Biochemistry, Ernst-Moritz-Arndt-University Greifswald, Felix-Hausdorff-Straße 4, 17487 Greifswald, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Lalk
authors.affiliation: Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Sauerbruchstr. DZ7, 17475 Greifswald, Germany.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Bröker
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schmidt
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany. Electronic address: voelker@uni-greifswald.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
mesh_terms: D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D011023:Pneumonia, Staphylococcal / Q000378:metabolism* / Q000382:microbiology; D020543:Proteome / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000378:metabolism*
chemical_list: D016207:Cytokines; D020543:Proteome
keywords: Epithelial cells; Flow cytometry; Host–pathogen interaction; Internalization; Metabolomics; Proteomics; Staphylococcus aureus