title: Sarcocystis oreamni, n. sp. (Apicomplexa: Sarcocystidae) from the mountain goat (Oreamnos americanus).
abstract: Numerous species of Sarcocystis have been reported from wild ruminants, but none has been named from the Rocky Mountain goat (Oreamnos americanus). Mature sarcocysts were found in frozen muscle samples of three of seven mountain goats from Alaska, USA. Two morphological types of sarcocysts were found; one had Sarcocystis cornagliai-like sarcocysts, previously named from the Alpine ibex (Capra ibex) from Europe. Two other goats were infected with a new species, Sarcocystis oreamni. Sarcocystis oreamni sarcocysts were microscopic with 2 μm-thick sarcocyst wall. By transmission electron microscopy, the sarcocyst wall had 1.7 μm-thick with unusual molar tooth-like villar protrusions (vp), type 29. The vp had an electron dense core and two disc-shaped plaques at the tip with fine microtubules. Bradyzoites were 8.6-9.1 μm long. Single nucleotide polymorphism (SNP) identified in 18S rRNA, and 28S rRNA loci of rDNA regions that suggested S. oreamni molecularly apart from related species. The phylogenetic analysis based on 18S rRNA and 28S rRNA sequences suggested S. oreamni is related with Sarcocystis species that employ members of the Canidae family as their definitive host.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: Calero-Bernal
authors.affiliation: Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa.
authors.forename: Erna
authors.initials: E
authors.lastname: Van Wilpe
authors.affiliation: Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.
authors.forename: Kevin
authors.initials: K
authors.lastname: White
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA.
authors.forename: Shiv K
authors.initials: SK
authors.lastname: Verma
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA.
authors.forename: Camila K
authors.initials: CK
authors.lastname: Cerqueira-Cézar
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA. jitender.dubey@ars.usda.gov.
authors.forename: Jitender P
authors.initials: JP
authors.lastname: Dubey
mesh_terms: D000413:Alaska; D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D005060:Europe; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D008969:Molecular Sequence Data; D010802:Phylogeny; D012522:Sarcocystis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000469:parasitology* / Q000662:veterinary
chemical_list: D004275:DNA, Ribosomal
keywords: Mountain goat; Oreamnos americanus; Phylogeny; Sarcocystis; Ultrastructure