title: Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells.
abstract: Deoxynivalenol (DON) is a mycotoxin affecting animals and plants. This toxin synthesized by Fusarium culmorum and Fusarium graminearum is currently believed to play a decisive role in the fungal phytopathogenesis as a virulence factor. Using cultured cells of Nicotiana tabacum BY2, we showed that DON-induced programmed cell death (PCD) could require transcription and translation processes, in contrast to what was observed in animal cells. DON could induce different cross-linked pathways involving (i) reactive oxygen species (ROS) generation linked, at least partly, to a mitochondrial dysfunction and a transcriptional down-regulation of the alternative oxidase (Aox1) gene and (ii) regulation of ion channel activities participating in cell shrinkage, to achieve PCD.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain, Paris, France; Ecole Normale Supérieure de Kouba, Laboratoire de Biologie de Systèmes Microbiens, Alger, Algeria; Institut National de la Recherche Agronomique d'Algérie, Centre de Recherche polyvalent Mehdi Boualem, Alger, Algeria.
authors.forename: Amine
authors.initials: A
authors.lastname: Yekkour
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tran
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain, Paris, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Arbelet-Bonnin
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain, Paris, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Briand
authors.affiliation: Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), ENSAT/INP de Toulouse, Castanet-Tolosan Cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Mathieu
authors.affiliation: Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), ENSAT/INP de Toulouse, Castanet-Tolosan Cedex, France; Université Moulay Ismail, Marjane 2, BP 298, Meknès, Maroc.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lebrihi
authors.affiliation: Université Moulay Ismail, Marjane 2, BP 298, Meknès, Maroc.
authors.forename: Rafik
authors.initials: R
authors.lastname: Errakhi
authors.affiliation: Ecole Normale Supérieure de Kouba, Laboratoire de Biologie de Systèmes Microbiens, Alger, Algeria.
authors.forename: Nasserdine
authors.initials: N
authors.lastname: Sabaou
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain, Paris, France. Electronic address: francois.bouteau@univ-paris-diderot.fr.
authors.forename: François
authors.initials: F
authors.lastname: Bouteau
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009183:Mycotoxins / Q000633:toxicity*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D013535:Suspensions; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D014255:Trichothecenes / Q000633:toxicity*; D000099188:Alternative Oxidase
chemical_list: D002118:Calcium; D007473:Ion Channels; D024101:Mitochondrial Proteins; D009183:Mycotoxins; D010088:Oxidoreductases; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013535:Suspensions; D014255:Trichothecenes; D000099188:Alternative Oxidase; C007262:deoxynivalenol
keywords: Deoxynivalenol; Fusarium; Ion channels; Nicotiana tabacum; Programmed cell death