title: Inoculation with endophytic Bacillus megaterium 1Y31 increases Mn accumulation and induces the growth and energy metabolism-related differentially-expressed proteome in Mn hyperaccumulator hybrid pennisetum.
abstract: In this study, a hydroponic culture experiment was conducted in a greenhouse to investigate the molecular and microbial mechanisms involved in the endophytic Bacillus megaterium 1Y31-enhanced Mn tolerance and accumulation in Mn hyperaccumulator hybrid pennisetum. Strain 1Y31 significantly increased the dry weights (ranging from 28% to 94%) and total Mn uptake (ranging from 23% to 112%) of hybrid pennisetum treated with 0, 2, and 10mM Mn compared to the control. Total 98 leaf differentially expressed proteins were identified between the live and dead bacterial inoculated hybrid pennisetum. The major leaf differentially expressed proteins were involved in energy generation, photosynthesis, response to stimulus, metabolisms, and unknown function. Furthermore, most of the energy generation and photosynthesis-related proteins were up-regulated, whereas most of the response to stimulus and metabolism-related proteins were down-regulated under Mn stress. Notably, the proportion of indole-3-acetic acid (IAA)-producing endophytic bacteria was significantly higher in the bacterial inoculated plants under Mn stress. The results suggested that strain 1Y31 increased the growth and Mn uptake of hybrid pennisetum through increasing the efficiency of photosynthesis and energy metabolism as well as the proportion of plant growth-promoting endophytic bacteria in the plants.
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Lin-Yan
authors.initials: LY
authors.lastname: He
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: xfsheng@njau.edu.cn.
authors.forename: Xia-Fang
authors.initials: XF
authors.lastname: Sheng
mesh_terms: D001410:Bacillus megaterium / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D052918:Environmental Restoration and Remediation; D018527:Hydroponics; D008345:Manganese / Q000378:metabolism*; D031782:Pennisetum / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism; D020543:Proteome / Q000187:drug effects*; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012989:Soil Pollutants; D008345:Manganese
keywords: Bacillus megaterium 1Y31; Differentially-expressed proteins; Mn accumulation; Mn hyperaccumulator hybrid pennisetum; Plant growth-promoting endophytic bacteria (PGPEB)