title: Therapeutic effect of the YH6 phage in a murine hemorrhagic pneumonia model.
abstract: The treatment, in farmed mink, of hemorrhagic pneumonia caused by multidrug-resistant Pseudomonas aeruginosa strains has become increasingly difficult. This study investigated the potential use of phages as a therapy against hemorrhagic pneumonia caused by P. aeruginosa in a murine hemorrhagic pneumonia model. An N4-like phage designated YH6 was isolated using P. aeruginosa strain D9. YH6 is a virulent phage with efficient and broad host lytic activity against P. aeruginosa. No bacterial virulence- or lysogenesis-related ORF is present in the YH6 genome, making it eligible for use in phage therapy. In our murine experiments, a single intranasal administration of YH6 (2 × 10(7) PFU) 2 h after D9 intranasal injections at double minimum lethal dose was sufficient to protect mice against hemorrhagic pneumonia. The bacterial load in the lungs of YH6-protected mice was less than 10(3) CFU/g within 24 h after challenge and ultimately became undetectable, whereas the amount of bacteria in the lung tissue derived from unprotected mice was more than 10(8) CFU/g within 24 h after challenge. In view of its protective efficacy in this murine hemorrhagic pneumonia model, YH6 may serve as an alternative treatment strategy for infections caused by multidrug-resistant P. aeruginosa.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Chongtao
authors.initials: C
authors.lastname: Du
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Gong
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, PR China.
authors.forename: Xinwu
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Ziyin
authors.initials: Z
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China. Electronic address: jingmin0629@163.com.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Gu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: hanwy@jlu.edu.cn.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D058491:Bacterial Load; D001691:Biological Therapy; D004195:Disease Models, Animal; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000628:therapy*; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D017105:Pseudomonas Phages / Q000302:isolation & purification / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity* / Q000821:virology
keywords: Bacteriophage; Mink; Pneumonia model; Pseudomonas aeruginosa