title: Ultrasensitive electrochemical biosensor based on reduced graphene oxide-tetraethylene pentamine-BMIMPF6 hybrids for the detection of α2,6-sialylated glycans in human serum.
abstract: In this paper, a simple, ultrasensitive and label-free electrochemical α2,6-sialylated glycans biosensor based on reduced graphene oxide-tetraethylene pentamine-1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) hybrids was developed. Due to the abundance of amino groups from reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) and the electrostatic interaction of BMIMPF6, bimetallic gold platinum alloy nanoparticles (AuPtNPs) were densely adsorbed onto the surface of the nanocomposite, providing a large surface area available for the immobilization of Sambucus nigra agglutinin (SNA). AuPtNPs have excellent conductivity and catalytic activity, which can promote electron transfer between the electrolyte solution and the surface of electrode and can enhance the sensitivity of biosensor. SNA, which specifically binds α2,6-sialylated glycans, was covalently immobilized on AuPtNPs for specific detection of α2,6-sialylated glycans in human serum. Under optimal experimental conditions, amperometric response changes were used to detect α2,6-sialylated glycans with a broad linear range of 10 fg mL(-1) -1 μg mL(-1) and a low detection limit of 3 fg mL(-1) (S/N=3). When applied to spiked serum samples, the recovery of the developed biosensor ranged from 100.8% to 101.4%, suggesting that the electrochemical biosensor would be suitable for the practical detection of α2,6-sialylated glycans.
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: yuchaom@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005029:Ethylenediamines / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D052578:Ionic Liquids / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000032:analysis* / Q000737:chemistry; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005029:Ethylenediamines; D052578:Ionic Liquids; D010087:Oxides; D011134:Polysaccharides; D006108:Graphite; D019158:N-Acetylneuraminic Acid; C034269:tetraethylenepentamine
keywords: BMIMPF(6); Bimetallic AuPt alloy nanoparticles; Sambucus nigra agglutinin; α2,6-sialylated glycans