title: Mechanism of Dimercaptosuccinic Acid Coated Superparamagnetic Iron Oxide Nanoparticles with Human Serum Albumin.
abstract: To research the mechanism of dimercaptosuccinic acid coated-superparamagnetic iron oxide nanoparticles (SPION) with human serum albumin (HSA), the methods of spectroscopy, molecular modeling calculation, and calorimetry were used in this paper. The inner filter effect of the fluorescence intensity was corrected to obtain the accurate results. Ultraviolet-visible absorption and circular dichroism spectra reflect that SPION changed the secondary structure with a loss of α-helix and loosened the protein skeleton of HSA; the activity of the protein was also affected by the increasing exposure of SPION. Fluorescence lifetime measurement indicates that the quenching mechanism type of this system was static quenching. The isothermal titration calorimetry measurement and molecular docking calculations prove that the predominant force of this system was the combination of Van der Waals' force and hydrogen bonds.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China -America CRC for Environment & Health, Shandong Province, Jinan, 250100, People's Republic of China. rutaoliu@sdu.edu.cn.
authors.forename: Lining
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China -America CRC for Environment & Health, Shandong Province, Jinan, 250100, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China -America CRC for Environment & Health, Shandong Province, Jinan, 250100, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, 27 Shandanan Road, Jinan, 250100, People's Republic of China.
authors.forename: Yunxing
authors.initials: Y
authors.lastname: Yan
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China -America CRC for Environment & Health, Shandong Province, Jinan, 250100, People's Republic of China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China -America CRC for Environment & Health, Shandong Province, Jinan, 250100, People's Republic of China.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D002151:Calorimetry; D002942:Circular Dichroism; D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D056004:Molecular Dynamics Simulation; D053758:Nanoparticles; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D004113:Succimer / Q000494:pharmacology*; D013816:Thermodynamics
chemical_list: D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; D012709:Serum Albumin; C000499:ferric oxide; D004113:Succimer
keywords: Human Serum Albumin; Isothermal Titration Calorimetry; Molecular Docking; Spectroscopy; Superparamagnetic Iron Oxide Nanoparticles