title: Acute and subchronic toxicity studies on safety assessment of Paecilomyces tenuipes N45 extracts.
abstract: Paecilomyces tenuipes, one of the commonly used Chinese medicinal fungus, has received much attention over the world, which possesses various active compounds and biological activities. However, little toxicological information is available. Therefore, the present study evaluated the potential toxicity of aqueous and ethanol extracts of Paecilomyces tenuipes N45 via acute and subchronic administration in mouse and rat, respectively. For improving the extraction rate of aqueous extract, response surface methodology (RSM) was employed to optimize the extraction condition first in this paper. The obtained optimal extract conditions were temperature 80 °C, liquid-solid ratio 50 mL·g-1 and time 3 h. In the acute toxicity test, aqueous and ethanol extracts caused neither mortality nor toxicological signs, and the maximum tolerance dose was estimated over 15 g/kg. No mortality or adverse effects was observed in subchronic toxicity studies. No significant difference in bodyweight, relative organ weight or hematological parameters was noted during the experiment. Comparing with nontreated rats, ALT, K and BUN levels were changed in experimental group detecting via biochemical analysis. No abnormality of internal organs was noted between treatment and control groups in gross and histopathological examinations. Our present study suggested that the tolerance dose of the Paecilomyces tenuipes N45 extracts were more than 15 g/kg and no-observed-adverse-effect level (NOAEL) of the extracts for both male and female rats after 90-day adminstation. Additionally, the extracts may possess renal-protective and hepato-protective effects.
authors.forename: Linna
authors.initials: L
authors.lastname: Du
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Chungang
authors.initials: C
authors.lastname: Liu
authors.forename: Qingfan
authors.initials: Q
authors.lastname: Meng
authors.forename: Jingjing
authors.initials: J
authors.lastname: Song
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.forename: Jiahui
authors.initials: J
authors.lastname: Lu
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Faculty of Life Sciences, Jilin University, P.O. Box: 130012, Changchun, China. tenglesheng@jlu.edu.cn.
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000633:toxicity; D005260:Female; D008297:Male; D020714:Maximum Tolerated Dose; D051379:Mice; D010143:Paecilomyces / Q000737:chemistry*; D051381:Rats; D023382:Toxicity Tests, Acute
chemical_list: D004365:Drugs, Chinese Herbal