title: Adding of ascorbic acid to the culture medium influences the antioxidant status and some biochemical parameters in the hen granulosa cells.
abstract: OBJECTIVE
  The aim of the present study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) of the hen granulosa cells, and selected biochemical parameters, including calcium, phosphorus, sodium, potassium, glucose, cholesterol, proteins, in the culture medium of granulosa cells after exposing them to ascorbic acid in vitro conditions.
  
  
  METHODS
  Ovarian granulosa cells of hens were incubated with various doses of ascorbic acid (E1 0.09 mg/ml, E2 0.13 mg/ml, E3 0.17 mg/ml, E4 0.33 mg/ml, E5 0.5 mg/ml).
  
  
  RESULTS
  Ascorbic acid did not manifest antioxidant potential and higher doses of ascorbic acid (0.17; 0.33 and 0.5 mg/ml) decreased the activity of SOD in granulosa cells. Vitamin application resulted in a significantly (p<0.05) higher accumulation of Na+ and K+ in culture media of granulosa cells and decreased the concentration of glucose and proteins.
  
  
  CONCLUSIONS
  These results indicate that ascorbic acid might be involved in the regulation of selected biochemical and physiological processes in ovarian granulosa cells.
authors.forename: M
authors.initials: M
authors.lastname: Capcarova
authors.forename: A
authors.initials: A
authors.lastname: Kolesarova
authors.forename: A
authors.initials: A
authors.lastname: Kalafova
authors.forename: J
authors.initials: J
authors.lastname: Bulla
authors.forename: A V
authors.initials: AV
authors.lastname: Sirotkin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005947:Glucose / Q000378:metabolism; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D003470:Culture Media; D011506:Proteins; D012964:Sodium; D013482:Superoxide Dismutase; D005947:Glucose; D001205:Ascorbic Acid; D011188:Potassium
keywords: antioxidants; ascorbic acid; biochemistry; granulosa cells; hen.