title: Cross-Excitation in Peripheral Sensory Ganglia Associated with Pain Transmission.
abstract: Despite the absence of synaptic contacts, cross-excitation of neurons in sensory ganglia during signal transmission is considered to be chemically mediated and appears increased in chronic pain states. In this study, we modulated neurotransmitter release in sensory neurons by direct application of type A botulinum neurotoxin (BoNT/A) to sensory ganglia in an animal model of neuropathic pain and evaluated the effect of this treatment on nocifensive. Unilateral sciatic nerve entrapment (SNE) reduced the ipsilateral hindpaw withdrawal threshold to mechanical stimulation and reduced hindpaw withdrawal latency to thermal stimulation. Direct application of BoNT/A to the ipsilateral L4 dorsal root ganglion (DRG) was localized in the cell bodies of the DRG and reversed the SNE-induced decreases in withdrawal thresholds within 2 days of BoNT/A administration. Results from this study suggest that neurotransmitter release within sensory ganglia is involved in the regulation of pain-related signal transmission.
authors.affiliation: Department of Stomatognathic Function and Occlusal Reconstruction, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan. c301351011@tokushima-u.ac.jp.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Omoto
authors.affiliation: Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan. maruhama@okayama-u.ac.jp.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Maruhama
authors.affiliation: Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan. ryujit@md.okayama-u.ac.jp.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Terayama
authors.affiliation: Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan. yumiya@md.okayama-u.ac.jp.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan. osamu@okayama-u.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Matsushita
authors.affiliation: Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan. tsugi@md.okayama-u.ac.jp.
authors.forename: Tomosada
authors.initials: T
authors.lastname: Sugimoto
authors.affiliation: Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8525, Japan. kuma@md.okayama-u.ac.jp.
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguma
authors.affiliation: Department of Stomatognathic Function and Occlusal Reconstruction, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan. matsuka@tokushima-u.ac.jp.
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Matsuka
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D019274:Botulinum Toxins, Type A / Q000627:therapeutic use*; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000502:physiology; D006358:Hot Temperature; D008297:Male; D009437:Neuralgia / Q000188:drug therapy* / Q000503:physiopathology; D010812:Physical Stimulation; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000601:surgery
chemical_list: D019274:Botulinum Toxins, Type A
keywords: botulinum toxin; dorsal root ganglion; mechanical allodynia; transmitter release