title: Effects of Mad Honey on Some Biochemical Parameters in Rats.
abstract: The aims of this study were to determine grayanotoxin (GTX-III) toxin level in mad honey using liquid chromatography-tandem mass spectrometry and examine the dynamic changes of certain biochemical parameters in blood serum of rats that consumed mad honey. For the experimental animal study, 20 Sprague-Dawley female rats were divided into 5 groups of 4 rats each, with one group being the control group (Group 1) and the others being the experimental groups (Groups 2-5). Groups 2, 3, 4, and 5 were, respectively, given mad honey extract at doses of 0.3, 0.6, 1.2, and 2.4 mg/g body weight/day via oral gavage for 8 days. According to results, the quantity of GTX-III found in the honey sample as 39.949 ± 0.020 μg GTX-III/g honey, and the biochemical analysis of the tested parameters (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase, creatine kinase, and creatine kinase muscle and brain) showed a significant elevation with increasing concentration of honey. In conclusion, the use of increasing concentrations of Rhododendron honey was seen as a source of enzymatic symptoms.
authors.affiliation: Giresun University, Giresun, Turkey huseyin.sahin@giresun.edu.tr.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Sahin
authors.affiliation: Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Oktay
authors.initials: O
authors.lastname: Yildiz
authors.affiliation: Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kolayli
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid; D004224:Diterpenes / Q000032:analysis*; D004573:Electrolytes / Q000097:blood; D004798:Enzymes / Q000097:blood; D005260:Female; D006722:Honey / Q000032:analysis* / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029793:Rhododendron; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D004224:Diterpenes; D004573:Electrolytes; D004798:Enzymes; C020751:grayanotoxin III
keywords: GTX-III; blood biomarkers; mad honey