title: The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces.
abstract: Fingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real-time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2-indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolated and resultant STR alleles when compared to control samples. DNA quantities and allele numbers were lower for samples enhanced with silver nitrate and 1,2-indanedione in acetic acid when compared to control samples. Based on DNA quantity, quality, and observable stochastic effects, our data indicated that iodine fuming and 1,2-indanedione in ethyl acetate were the preferred options for the enhancement of fingerprints on porous surfaces.
authors.affiliation: Department of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan, 33304, Taiwan.
authors.forename: Li-Chin
authors.initials: LC
authors.lastname: Tsai
authors.affiliation: Department of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan, 33304, Taiwan.|Forensic Science Center, New Taipei City Police Department, No. 32, Fuzhong Rd., Banqiao District, New Taipei City, 22005, Taiwan.
authors.forename: Cheng-Chang
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Department of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan, 33304, Taiwan.|Forensic Science Section, Changhua County Police Bureau, 778, Sec. 2, Jhong-jheng Rd., Changhua City, 50004, Taiwan.
authors.forename: Chun-Chieh
authors.identifier: http://orcid.org/0000-0002-0362-2396
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Forensic Medicine, College of Medicine, National Taiwan University, No. 1 Jen-Ai Road Section 1, Taipei, 10051, Taiwan.
authors.forename: James Chun-I
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan, 33304, Taiwan.
authors.forename: Sheng-Meng
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Forensic Science Center, New Taipei City Police Department, No. 32, Fuzhong Rd., Banqiao District, New Taipei City, 22005, Taiwan.
authors.forename: Nu-En
authors.initials: NE
authors.lastname: Huang
authors.affiliation: School of Biological Sciences, Flinders University, Adelaide, SA, 5001, Australia.
authors.forename: Adrian
authors.initials: A
authors.lastname: Linacre
authors.affiliation: Department of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan, 33304, Taiwan.
authors.forename: Hsing-Mei
authors.initials: HM
authors.lastname: Hsieh
mesh_terms: D016172:DNA Fingerprinting; D003878:Dermatoglyphics; D004847:Epithelial Cells; D044707:Forensic Sciences; D006801:Humans; D007189:Indans
chemical_list: C413384:1,2-indanedione; D007189:Indans
keywords: DNA typing; epithelial cells; fingerprint enhancement; forensic science; porous surfaces; stochastic effects