title: Osteoclast Deficiency Contributes to Temporomandibular Joint Ankylosed Bone Mass Formation.
abstract: Ankylosed bone mass in temporomandibular joint ankylosis (TMJA) is an important factor affecting mouth-opening limitation. However, the mechanism underlying the occurrence of ankylosed bone mass remains unknown. Research has shown that osteoblasts and osteoclasts maintain balance in bone remodeling. Thus, we hypothesized that aberrant osteoclastogenesis and osteogenesis may be involved in the occurrence of ankylosed bone mass in TMJA. In this study, we characterized the osteogenesis of bone marrow stem cells and the osteoclastogenesis of myelomonocyte in clinical specimens of TMJA and normal controls. Results showed that, compared with control bone marrow stem cells, TMJA bone marrow stem cells had lower proliferative and osteogenic capacities. The number of osteoclasts in the ankylosed bone mass group dramatically decreased, and myelomonocyte osteoclastogenic potential was impaired. The RANKL/OPG ratio of the ankylosed bone mass group was lower than that of the control group. Thus, our study suggests that osteoclast deficiency may be an important factor affecting bone mass ankylosis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China.
authors.forename: L H
authors.initials: LH
authors.lastname: He
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China.
authors.forename: E
authors.initials: E
authors.lastname: Xiao
authors.affiliation: Department of General Dentistry, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China.
authors.forename: D H
authors.initials: DH
authors.lastname: Duan
authors.affiliation: Central Laboratory and Center for TMD and Orofacial Pain, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Gan
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China kqyehuagan@bjmu.edu.cn.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000844:Ankylosis / Q000000981:diagnostic imaging / Q000209:etiology*; D015519:Bone Density; D006801:Humans; D010010:Osteoclasts / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D013704:Temporomandibular Joint / Q000000981:diagnostic imaging / Q000503:physiopathology; D013705:Temporomandibular Joint Disorders / Q000000981:diagnostic imaging / Q000209:etiology*; D014057:Tomography, X-Ray Computed
keywords: bone marrow myelomonocytes; bone marrow stem cells; bone remodeling; osteoclastogenesis; osteogenesis; pathogenesis