title: Acinetobacter albensissp. nov., isolated from natural soil and water ecosystems.
abstract: We have studied the taxonomic position of a phenetically unique group of eight strains of the genusAcinetobacterwhich were isolated from soil and water samples collected in protected landscape areas in the Czech Republic. Each of the comparative sequence analyses of the 16S rRNA,gyrBandrpoBgenes showed that the eight strains formed a cohesive and tight cluster (intracluster sequence identities of ≥ 99.9 %, ≥ 98.5 % and ≥ 97.7 %, respectively), which was clearly separated from all hitherto known species of the genusAcinetobacter( ≤ 98.6 %, ≤ 84.5 % and ≤ 89.3 %, respectively). Congruent with these findings were the results of comparative sequence analysis of three additional housekeeping genes (gltA,pyrGandrecA). This genotypic distinctness was mirrored by the uniqueness of the combination of a number of independent phenotypic markers including the whole-cell spectra produced by matrix-assisted laser desorption ionization time-of-flight (MALDI-ToF) MS and physiological and metabolic features. The most useful phenotypic features to differentiate the eight strains from all known species of the genusAcinetobacterwere the ability to assimilate tricarballylate and the inability to grow at 35 °C or to assimilate ethanol or l-histidine. We conclude that the eight strains represent a novel environmental species for which the nameAcinetobacter albensissp. nov. is proposed. The type strain is ANC 4874T( = CCUG 67281T= CCM 8611T).
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Krizova
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Maixnerova
authors.affiliation: Research Group Proteomics, Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Sedo
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
authors.forename: Alexandr
authors.initials: A
authors.lastname: Nemec