title: Spontaneous and induced labour are associated with different myometrial proteomes in the human.
abstract: Human myometrium undergoes a major phenotypic change at labour likely involving modifications to key regulatory proteins. In some cases, the myometrium fails to activate normally and medical intervention is required to induce labour. In this study, 2-D DIGE was used to examine changes in the myometrial proteome at the time of spontaneous (SL) and induced labour (IL). Proteomic profiles of nonlabouring term myometria (NL, n = 6) were quantitatively compared to SL (n = 6) and prostaglandin/oxytocin-IL term myometria (n = 6). In SL samples, 23 differentially expressed protein spots were detected (9 increased/14 decreased compared to NL, p<0.05). In IL samples, 59 differentially expressed spots were observed (13 increased/46 decreased compared to NL). Comparison of SL and IL proteomes revealed 69 differentially expressed proteins (7 increased/62 decreased). Two proteins consistently decreased in SL and IL samples were identified as transgelin (1.98- and 1.97-fold decrease in SL and IL, respectively) and αB-crystallin (3.27- and 2.49-fold decrease). Levels of desmin and cytosolic phospholipase A2 β were decreased 2.9- and 2.65-fold, respectively only in IL samples. Our results show human labour is accompanied by general downregulation of specific myometrial proteins. Differences exist between SL and IL myometrial proteomes indicating divergence of underlying processes and highlighting the importance of distinguishing these groups in future studies of parturition. Our findings underscore the utility of discovery approaches in investigations of organ-wide protein changes that underlie discrete physiological events including human labour.
authors.affiliation: Mothers and Babies Research Centre, Hunter Medical Research Institute, The University of Newcastle, John Hunter Hospital, Newcastle, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: MacIntyre
authors.affiliation: Mothers and Babies Research Centre, Hunter Medical Research Institute, The University of Newcastle, John Hunter Hospital, Newcastle, Australia.
authors.forename: Roger
authors.initials: R
authors.lastname: Smith
authors.affiliation: KK Women's and Children's Hospital, Singapore, Singapore.
authors.forename: George
authors.initials: G
authors.lastname: Yeo
authors.affiliation: KK Women's and Children's Hospital, Singapore, Singapore.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Kwek
authors.affiliation: Mothers and Babies Research Centre, Hunter Medical Research Institute, The University of Newcastle, John Hunter Hospital, Newcastle, Australia.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Bisits
authors.affiliation: Mothers and Babies Research Centre, Hunter Medical Research Institute, The University of Newcastle, John Hunter Hospital, Newcastle, Australia. Cheng.Chan@newcastle.edu.au.
authors.forename: Eng-Cheng
authors.initials: EC
authors.lastname: Chan
keywords: 2‐D DIGE; Human myometrium; Labour