title: Phytochemical analysis and effects of Pteris vittata extract on visual processes.
abstract: The present study was designed to explore the possible effects of Pteris vittata on visual sensitivity, ERG waves, and other components of the visual system. Electrophysiological techniques including electroretinography (ERG) were used in the present study. The phytochemical composition of the extract was investigated using liquid chromatography-mass spectrometry (LC-MS) techniques. The results indicated that the extract significantly augmented dark- and light-adapted ERG b-wave amplitude. Furthermore, these findings showed that P. vittata extract does not have Gamma-aminobutyric acid receptor antagonistic activity but may function as a retinal neural antagonist in bullfrog retina. P. vittata extract improved the visual sensitivity by 0.8 log unit of light intensity, and reduced the regeneration time for rhodopsin. The six main peaks obtained through LC-MS were identified as flavonoids. Based on these results, it was concluded that P. vittata extract or its constituents may be used to treat eye diseases.
authors.affiliation: Biotechnology Program, Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan. fazliwahid@ciit.net.pk.
authors.forename: Fazli
authors.initials: F
authors.lastname: Wahid
authors.affiliation: Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan.
authors.forename: Taous
authors.initials: T
authors.lastname: Khan
authors.affiliation: Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan.
authors.forename: Omer
authors.initials: O
authors.lastname: Shehzad
authors.affiliation: Department of Biomedical Engineering, National University of Sciences and Technology, Islamabad, Pakistan.
authors.forename: Adeeb
authors.initials: A
authors.lastname: Shehzad
authors.affiliation: School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, South Korea. yykim@knu.ac.kr.
authors.forename: You Young
authors.initials: YY
authors.lastname: Kim
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002853:Chromatography, Liquid; D004596:Electroretinography / Q000187:drug effects*; D018756:GABA Antagonists / Q000494:pharmacology; D007736:Kynurenic Acid / Q000494:pharmacology; D008027:Light; D013058:Mass Spectrometry; D064209:Phytochemicals; D010852:Picrotoxin / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D032495:Pteris / Q000378:metabolism*; D011892:Rana catesbeiana; D012160:Retina / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D014785:Vision, Ocular / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D018756:GABA Antagonists; D007736:Kynurenic Acid; D064209:Phytochemicals; D010852:Picrotoxin; D010936:Plant Extracts; D012243:Rhodopsin; D005680:gamma-Aminobutyric Acid
keywords: Brake fern; Electroretinography; Eye; Flavonoids; Pteris vittata L. (Pteridaceae); Visual system