title: Lysine-doped polypyrrole/spider silk protein/poly(l-lactic) acid containing nerve growth factor composite fibers for neural application.
abstract: Lysine-doped polypyrrole (PPy)/regenerated spider silk protein (RSSP)/poly(l-lactic) acid (PLLA)/nerve growth factor (NGF) (L-PRPN) composite scaffold was fabricated by co-axial electrospraying and electrospinning. This L-PRPN composite scaffold had a structure of microfibers with a core-shell structure as the stems and nanofibers as branches. Assessment in vitro demonstrated that the L-PRPN composite micro/nano-fibrous scaffold could maintain integrated structure for at least 4months and the pH value of PBS at about 7.28. It had good biocompatibility and cell adhesion and relatively stable conductivity. PC 12 cells cultured on this scaffold, anisotropic cell-neurite-cell-neurite or neurite-neurite sheets were formed after being cultured for 6days. Evaluations in vivo also showed that L-PRPN composite fibrous conduit was effective at bridging 2.0cm sciatic nerve gap in adult rat within 10months. This conduit and electrical stimulation (ES) through it promoted Schwann cell migration and axonal regrowth.
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Faculty of Science, University of Kanagawa, Hiratsuka-shi, Kanagawa 259-1293, Japan.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, People's Republic of China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Xing
authors.affiliation: College of Materials and Textile Engineering, Jiaxing Key Lab of Medical Molecular Biology, Zhejiang Experimental Center of Materials and Textile Engineering, Jiaxing University, Jiaxing Zhejiang 314001, People's Republic of China. Electronic address: w2003yqz@126.com.
authors.forename: Qiaozhen
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D008297:Male; D057139:Nanofibers / Q000737:chemistry*; D020932:Nerve Growth Factor / Q000737:chemistry*; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism; D016716:PC12 Cells; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism; D047011:Silk / Q000737:chemistry*; D013112:Spiders; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; D011758:Pyrroles; D047011:Silk; C067635:polypyrrole; D019344:Lactic Acid; C033616:poly(lactide); D020932:Nerve Growth Factor; D008239:Lysine
keywords: Electrical stimulation; Peripheral nerve regeneration; Polypyrrole; Regenerated spider silk protein