title: The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma.
abstract: Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine the lncRNA expression profile in astrocytomas and to assess its potential clinical value. We performed a three-step analysis to establish the lncRNA profile for astrocytoma: a) the lncRNA expression was examined on 3 astrocytomas as well as 3 NATs (normal adjacent tissues) using the lncRNA microarray; b) the top-hits were validated in 40 astrocytomas (WHO grade II-IV) by quantitative real time-PCR (qRT-PCR); c) the hits with significant differences were re-evaluated using qRT-PCR in 90 astrocytomas. Finally, 7 lncRNAs were found to have a significantly different expression profile in astrocytoma samples compared to the NAT samples. Unsupervised clustering analysis further revealed the potential of the 7-lncRNA profile to differentiate between tumors and NAT samples. The upregulation of ENST00000545440 and NR_002809 was associated with advanced clinical stages of astrocytoma. Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival. Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors. Our results indicate that the lncRNA profile may be a potential prognostic biomarker for the prediction of post-surgical outcomes.
authors.affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhi
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Naiyuan
authors.initials: N
authors.lastname: Shao
authors.affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Danni
authors.initials: D
authors.lastname: Deng
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Suinuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Xia
authors.affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000328:Adult; D051398:Aquaporin 1 / Q000378:metabolism; D001254:Astrocytoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D016000:Cluster Analysis; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D054648:Protein Phosphatase 2 / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics*; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D019063:Tenascin / Q000378:metabolism; D015854:Up-Regulation; D000099249:Proteoglycan Link Protein
chemical_list: D051398:Aquaporin 1; D014408:Biomarkers, Tumor; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D054648:Protein Phosphatase 2; D062085:RNA, Long Noncoding; D019063:Tenascin; C480571:HAPLN4 protein, human; D000099249:Proteoglycan Link Protein; C517192:PPP2R2C protein, human