title: In vitro fermentation of lupin seeds (Lupinus albus) and broad beans (Vicia faba): dynamic modulation of the intestinal microbiota and metabolomic output.
abstract: Broad beans (Vicia faba) and lupin seeds (Lupinus albus) are legumes rich in a wide range of compounds, which may represent a useful dietary approach for modulating the human gut microbiome. In this work, after in vitro digestion, legume samples were used as carbon sources in anaerobic batch cultures to evaluate their impact on the intestinal microbiota composition and on their metabolic products. The fermentations were monitored by a decrease in pH, generation of short chain fatty acids (SCFA) and lactate and the changes in the dynamic bacterial populations by fluorescence in situ hybridization (FISH). The total SCFA at the end of fermentation was 81.52 mM for lupin seeds and 78.41 mM for broad beans accompanied by a decrease of the pH for both legumes. The microbial groups that increased significantly (P < 0.05) were Bifidobacterium spp., Lactobacillus-Enterococcus, Atopobium, Bacteroides-Pretovella, Clostridium coccoides-Eubacterium rectale, Faecalibacterium prausnitzii and Roseburia intestinalis. This impact on the intestinal microbiota suggests that lupin seeds and broad beans may be used in the development of novel functional foods, which can be included in dietary strategies for human health promotion.
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal. beatriz.gullon@usc.es.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gullón
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gullón
authors.forename: Freni
authors.initials: F
authors.lastname: Tavaria
authors.forename: Marta
authors.initials: M
authors.lastname: Vasconcelos
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Gomes
mesh_terms: D001439:Bacteroides / Q000302:isolation & purification; D001644:Bifidobacterium / Q000302:isolation & purification; D003013:Clostridium / Q000302:isolation & purification; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017404:In Situ Hybridization, Fluorescence; D007778:Lactobacillus / Q000302:isolation & purification; D031297:Lupinus / Q000737:chemistry*; D055432:Metabolomics; D028321:Plant Preparations / Q000494:pharmacology; D056692:Prebiotics; D012639:Seeds / Q000737:chemistry*; D014675:Vegetables; D031307:Vicia faba / Q000737:chemistry*
chemical_list: D005232:Fatty Acids, Volatile; D028321:Plant Preparations; D056692:Prebiotics