title: Simultaneous determination of polyvinylchloride plasticizers di(2-ethylhexyl) phthalate and tri(2-ethylhexyl) trimellitate and its degradation products in blood by liquid chromatography-tandem mass spectrometry.
abstract: Di(2-ethylhexyl) phthalate (DEHP) and tri(2-ethylhexyl) trimellitate (TEHTM or TOTM) are common plasticizers that are also largely used for PVC medical devices, e.g. bags and tubing for blood transfusions and infusions. The leachability of medical devices is a well-known situation of increasing toxicological concern. To assess the migration of plasticizers from PVC medical devices into human blood we developed and validated an analytical method for the determination of DEHP and TOTM in combination with the determination of their primary degradation products mono(2-ethylhexyl) phthalate (MEHP), 1,2-di(2-ethylhexyl) trimellitate (1,2-DEHTM) and 2-mono(2-ethylhexyl) trimellitate (2-MEHTM). The presented method involves liquid-liquid extraction of the analytes from the blood followed by the subsequent analytical separation and detection using LC-MS/MS analysis. The validation of the procedure showed a good precision in the range of 1.8 to 5.3%. Mean accuracy ranged from 86% for 1,2-DEHTM to 109% for MEHP. LOQ was found to be 2 to 5μg/L for each of the analytes. Additionally, the method is characterised by its wide linear range up to 2mg/L each for the degradation products of TOTM to 100mg/L for the parent plasticizer DEHP. The presented method promises to be of major advantage for further studies as it allows for the first time the simultaneous determination of DEHP and TOTM in human blood in combination with the analysis of their degradation products that render possible to investigate the leachability of a broad range of PVC medical devices in human blood using only one analytical method.
authors.affiliation: Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 Erlangen, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Eckert
authors.affiliation: Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 Erlangen, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Müller
authors.affiliation: Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 Erlangen, Germany. Electronic address: Thomas.Goeen@fau.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Göen
mesh_terms: D001565:Benzoates / Q000097:blood*; D001711:Biotransformation; D002853:Chromatography, Liquid; D004051:Diethylhexyl Phthalate / Q000097:blood*; D006801:Humans; D006868:Hydrolysis; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry; D010968:Plasticizers / Q000032:analysis*; D011143:Polyvinyl Chloride / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D001565:Benzoates; D010968:Plasticizers; C036101:tri-(2-ethylhexyl)trimellitate; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate
keywords: DOP; Medical device safety; Migration; PVC products; TEHTM