title: Characterization of metabolic profile of intact non-tumor and tumor breast cells by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy.
abstract: (1)H high-resolution magic angle spinning nuclear magnetic resonance ((1)H HR-MAS NMR) spectroscopy was used to analyze the metabolic profile of an intact non-tumor breast cell line (MCF-10A) and intact breast tumor cell lines (MCF-7 and MDA-MB-231). In the spectra of MCF-10A cells, six metabolites were assigned, with glucose and ethanol in higher concentrations. Fifteen metabolites were assigned in MCF-7 and MDA-MB-231 (1)H HR-MAS NMR spectra. They did not show glucose and ethanol, and the major component in both tumor cells was phosphocholine (higher in MDA-MB-231 than in MCF-7), which can be considered as a tumor biomarker of breast cancer malignant transformation. These tumor cells also show acetone signal that was higher in MDA-MB-231 cells than in MCF-7 cells. The high acetone level may be an indication of high demand for energy in MDA-MB-231 to maintain cell proliferation. The higher acetone and phosphocholine levels in MDA-MB-231 cells indicate the higher malignance of the cell line. Therefore, HR-MAS is a rapid reproducible method to study the metabolic profile of intact breast cells, with minimal sample preparation and contamination, which are critical in the analyses of slow-growth cells.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP 13560-970, Brazil; Embrapa Instrumentação, São Carlos, SP 13560-970, Brazil.
authors.forename: Roberta M
authors.initials: RM
authors.lastname: Maria
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP 13560-970, Brazil.
authors.forename: Wanessa F
authors.initials: WF
authors.lastname: Altei
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP 13560-970, Brazil.
authors.forename: Adriano D
authors.initials: AD
authors.lastname: Andricopulo
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Centro de Ciências Biológicas e da Saúde/Universidade Federal de São Carlos (CCBS/UFSCar), Departamento de Gerontologia, Laboratório de Biologia do Envelhecimento (LABEN), São Carlos, SP 13565-905, Brazil.
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Becceneri
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Centro de Ciências Biológicas e da Saúde/Universidade Federal de São Carlos (CCBS/UFSCar), Departamento de Gerontologia, Laboratório de Biologia do Envelhecimento (LABEN), São Carlos, SP 13565-905, Brazil.
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Cominetti
authors.affiliation: Departamento de Química, Centro de Ciências Exatas e de Tecnologia, Universidade Federal de São Carlos, São Carlos, SP 13565-905, Brazil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Venâncio
authors.affiliation: Embrapa Instrumentação, São Carlos, SP 13560-970, Brazil. Electronic address: luiz.colnago@embrapa.br.
authors.forename: Luiz A
authors.initials: LA
authors.lastname: Colnago
mesh_terms: D000096:Acetone / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D000431:Ethanol / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular; D010767:Phosphorylcholine / Q000378:metabolism
chemical_list: D015415:Biomarkers; D010767:Phosphorylcholine; D000096:Acetone; D000431:Ethanol; D005947:Glucose
keywords: Breast cancer; HR–MAS; Metabolic profile; Metabolomics; NMR