title: Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062.
abstract: The present study addresses growth parameters and physiological and biochemical characteristics of the aerobic CO-oxidizing carboxydobacterium Seliberia carboxydohydrogena Z-1062. Poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of S. carboxydohydrogena Z-1062 grown on gas mixtures consisting of CO(2), O(2), H(2), and CO. CO concentrations of 10, 20, and 30 % v/v did not affect polymer synthesis, whose content after 56-h cultivation under limiting concentrations of nitrogen and sulfur was 52.6-62.8 % of biomass weight at a productivity of 0.13-0.22 g/L h. The inhibitory effect of CO on cell concentration was revealed at CO concentration of 30 % v/v. That also caused a decrease in substrate (H(2) and O(2)) use efficiency. Thus, this carboxydobacterium can be regarded as a potential producer of polyhydroxyalkanoates from industrial hydrogenous sources.
authors.affiliation: Institute of Biophysics SB RAS, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation. volova45@mail.ru.|Siberian Federal University, 79 Svobodny Ave., Krasnoyarsk, 660041, Russian Federation. volova45@mail.ru.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Volova
authors.affiliation: Institute of Biophysics SB RAS, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation.|Siberian Federal University, 79 Svobodny Ave., Krasnoyarsk, 660041, Russian Federation.
authors.forename: Natalia
authors.initials: N
authors.lastname: Zhila
authors.affiliation: Institute of Biophysics SB RAS, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation.|Siberian Federal University, 79 Svobodny Ave., Krasnoyarsk, 660041, Russian Federation.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Shishatskaya
mesh_terms: D018533:Biomass; D002248:Carbon Monoxide / Q000378:metabolism* / Q000494:pharmacology; D005740:Gases / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D006885:Hydroxybutyrates / Q000032:analysis / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011091:Polyesters / Q000032:analysis / Q000378:metabolism*; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D000094122:Polyhydroxybutyrates
chemical_list: D002248:Carbon Monoxide; D005740:Gases; D006859:Hydrogen; D006885:Hydroxybutyrates; D009584:Nitrogen; D010100:Oxygen; D011091:Polyesters; D013455:Sulfur; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Carbon monoxide; Carboxydobacteria; Poly(3-hydroxybutyrate); Synthesis