title: Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study.
abstract: Although zinc oxide nanoparticles (ZnONPs) are recognized to cause systemic disorders, little is known about the mechanisms that underlie the time-dependent differences that occur after exposure. The objective of this study was to investigate the mechanistic differences at 24 hours and 28 days after the exposure of BALB/c mice to ZnONPs via intratracheal instillation. An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways. A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein. Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times. The glycolysis/gluconeogenesis pathway was continuously downregulated from 24 hours to 28 days, whereas detoxification pathways were activated at the 28-day time-point after exposure. A comprehensive understanding of the potential time-dependent effects of exposure to ZnONPs was provided, which highlights the metabolic mechanisms that may be important in the responses to ZnONP.
authors.affiliation: Institute of Occupational Safety and Health, Council of Labor Affairs, Executive Yuan, Taiwan ; School of Public Health, National Defense Medical Center, Taipei Medical University, Taipei, Taiwan.
authors.forename: Chih-Hong
authors.initials: CH
authors.lastname: Pan
authors.affiliation: School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan ; Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Kai-Jen
authors.initials: KJ
authors.lastname: Chuang
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Jen-Kun
authors.initials: JK
authors.lastname: Chen
authors.affiliation: Graduate Institute of Environmental Engineering, National Central University, Taoyuan, Taiwan.
authors.forename: Ta-Chih
authors.initials: TC
authors.lastname: Hsiao
authors.affiliation: School of Public Health, National Defense Medical Center, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ching-Huang
authors.initials: CH
authors.lastname: Lai
authors.affiliation: School of Earth and Ocean Sciences, Cardiff University, Cardiff, Wales, UK.
authors.forename: Tim P
authors.initials: TP
authors.lastname: Jones
authors.affiliation: School of Biosciences, Cardiff University, Cardiff, Wales, UK.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: BéruBé
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Gui-Bing
authors.initials: GB
authors.lastname: Hong
authors.affiliation: Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, People's Republic of China ; Shenzhen Municipal Key Laboratory for Health Risk Analysis, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Kin-Fai
authors.initials: KF
authors.lastname: Ho
authors.affiliation: School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan ; Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hsiao-Chi
authors.initials: HC
authors.lastname: Chuang
mesh_terms: D000818:Animals; D005500:Follow-Up Studies; D008168:Lung / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D018675:Toxicity Tests; D015034:Zinc Oxide / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D015034:Zinc Oxide
keywords: S100-A9; glycolysis; iTRAQ; metabolism; nanoparticles