title: Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors.
abstract: Primary hepatic carcinoma (PHC) is one of the most common malignancies worldwide, resulting in death within six to 20 months. The survival rate can be improved by effective treatments when diagnosed at an early stage. The α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) have been identified as markers that are expressed at higher levels in PHC patients. In this study, we employed silicon nanowire field-effect transistors (SiNW-FETs) with polydimethylsiloxane (PDMS) microfluidic channels to simultaneously detect AFP and CEA in desalted human serum. Dual-channel PDMS was first utilized for the selective modification of AFP and CEA antibodies on SiNWs, while single-channel PDMS offers faster and more sensitive detection of AFP and CEA in serum. During the SiNW modification process, 0.1% BSA was utilized to minimize nonspecific protein binding from serum. The linear dynamic ranges for the AFP and CEA detection were measured to be 500 fg/mL to 50 ng/mL and 50 fg/mL to 10 ng/mL, respectively. Our work demonstrates the promising potential of fabricated SiNW-FETs as a direct detection kit for multiple tumor markers in serum; therefore, it provides a chance for early stage diagnose and, hence, more effective treatments for PHC patients.
authors.affiliation: Nanjing Medical University, Nanjing 210029, China. kuiyuzhu4@163.com.
authors.forename: Kuiyu
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Wuxi People\'s Hospital, Wuxi 214023, China. yezhang4555@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Nanjing Medical University, Nanjing 210029, China. zengyaoli44@163.com.
authors.forename: Zengyao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Nanjing Medical University, Nanjing 210029, China. fanzhou4@163.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Nanjing Medical University, Nanjing 210029, China. kangfeng44@163.com.
authors.forename: Kang
authors.initials: K
authors.lastname: Feng
authors.affiliation: Wuxi People\'s Hospital, Wuxi 214023, China. douhuiqiang4@163.com.
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Dou
authors.affiliation: Nanjing Medical University, Nanjing 210029, China. wangtong@wuxiph.com.|Wuxi People\'s Hospital, Wuxi 214023, China. wangtong@wuxiph.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D002138:Calibration; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D004560:Electricity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053770:Nanowires / Q000737:chemistry*; D061848:Optical Imaging; D012825:Silicon / Q000737:chemistry*; D014173:Transistors, Electronic; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D002272:Carcinoembryonic Antigen; D000509:alpha-Fetoproteins; D012825:Silicon
keywords: carcinoembryonic antigen; polydimethylsiloxane microfluidic channel; primary hepatic carcinoma; silicon nanowire field-effect transistor; α-fetoprotein