title: miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A.
abstract: MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention.
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China.
authors.forename: Mingchen
authors.initials: M
authors.lastname: Shao
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Gastrointestinal Surgery Department, People's Hospital of Zhengzhou, Zhengzhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Liver Transplantation Hepatobiliary Surgery Department, People's Hospital of Zhengzhou, Zhengzhou, China.
authors.forename: Sidong
authors.initials: S
authors.lastname: Wei
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China.
authors.forename: Liuxing
authors.initials: L
authors.lastname: Wang
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ma
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D065646:Thyroid Carcinoma, Anaplastic / Q000378:metabolism* / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; C000602903:MIRN4295 microRNA, human; D035683:MicroRNAs
keywords: Anaplastic thyroid carcinoma; CDKN1A; miR-4295