title: Nitrile Oxide-Norbornene Cycloaddition as a Bioorthogonal Crosslinking Reaction for the Preparation of Hydrogels.
abstract: This communication describes the first application of cycloaddition between an in situ generated nitrile oxide with norbornene leading to a polymer crosslinking reaction for the preparation of poly(ethylene glycol) hydrogels under physiological conditions. Hydrogels with high water content and robust physical strength are readily formed within 2-5 min by a simple two-solution mixing method which allows 3D encapsulation of neuronal cells. This bioorthogonal crosslinking reaction provides a simple yet highly effective method for preparation of hydrogels to be used in bioengineering.
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton, 3800, VIC, Australia.
authors.forename: Vinh X
authors.initials: VX
authors.lastname: Truong
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton, 3800, VIC, Australia.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton, 3800, VIC, Australia.
authors.forename: George P
authors.initials: GP
authors.lastname: Simon
authors.affiliation: Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton, 3800, VIC, Australia.
authors.forename: John S
authors.initials: JS
authors.lastname: Forsythe
mesh_terms: D000818:Animals; D002460:Cell Line; D061565:Cycloaddition Reaction; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D009570:Nitriles / Q000737:chemistry*; D009636:Norbornanes / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D012212:Rheology
chemical_list: D020100:Hydrogels; D009570:Nitriles; D009636:Norbornanes; D010087:Oxides
keywords: 3D cell encapsulation; bioorthogonal click reactions; hydrogels