title: Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination.
abstract: The abundance of biochemical and structural knowledge on the Cyclin-Dependent Kinases (CDKs) has provided a comprehensive but not exhaustive insight into the molecular determinants that govern their function mechanisms. The implementation of structural and functional CDK models towards developing novel anticancer strategies that will specifically target individual or multiple CDKs remains a critical need.More than 250 CDKs crystal structures are available to-date, including truncated or whole, modified or not, active or inactive forms, co-crystallized with the cyclins and/or their respective putative inhibitors, though, to our knowledge, there is no NMR solved structure available to date. We hitherto attempt to provide a useful guide from protein production to crystallization for CDK/Inhibitors complexes based on an overview of the already elucidated CDK structures, constructs and the preferable expression vectors in each case, in order to yield the respective crystals.
authors.affiliation: Department of Biochemistry, Veterinary School, University of Thessaly, Trikalon 224 Str., Karditsa, 43100, Greece.
authors.forename: Asterios I
authors.initials: AI
authors.lastname: Grigoroudis
authors.forename: George
authors.initials: G
authors.lastname: Kontopidis
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D018844:Cyclin-Dependent Kinases / Q000737:chemistry*; D016213:Cyclins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D030562:Databases, Protein; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007313:Insecta; D009682:Magnetic Resonance Spectroscopy; D008967:Molecular Biology / Q000379:methods; D010766:Phosphorylation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D016213:Cyclins; D018844:Cyclin-Dependent Kinases