title: Fibroblast growth factor receptor 1 gene amplification in gastric adenocarcinoma.
abstract: Gastric adenocarcinomas are associated with a poor prognosis due to the fact that the tumor has often metastasized by the time of diagnosis. Thus, identification of novel therapeutic targets is highly desirable. Here, we examined gene copy number of fibroblast growth factor receptor 1 (FGFR1), a potential target for tyrosine kinase inhibitors, and clinicopathologic parameters in a large cohort of gastric adenocarcinomas. We performed fluorescence in situ hybridization analysis of 293 gastric adenocarcinomas using tissue microarrays. Amplification of the FGFR1 gene is a rare but noticeable event that can be found in 2% (6/293) of cases and was associated with poor 10-year survival (median 15.3 months in FGFR1-amplified cases versus 36 months in nonamplified cases, P = .047) and a higher rate of distant metastasis (P = .025). FGFR1 appears to represent a potential new therapeutic target in a subset of patients with gastric carcinoma. Identification of gastric cancers harboring FGFR1 amplification may be important in preselecting patients and/or interpreting clinical studies using tyrosine kinase inhibitors.
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Manuel H
authors.initials: MH
authors.lastname: Schäfer
authors.affiliation: Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Department of General, Visceral, Thoracic and Vascular Surgery, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Lingohr
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Sträßer
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Nils C
authors.initials: NC
authors.lastname: Lehnen
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Department of Prostate Cancer Research, Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Braun
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Department of Prostate Cancer Research, Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Perner
authors.affiliation: Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Institute of Medical Biometry, Informatics and Epidemiology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Höller
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Glen
authors.initials: G
authors.lastname: Kristiansen
authors.affiliation: Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Department of General, Visceral, Thoracic and Vascular Surgery, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Jörg C
authors.initials: JC
authors.lastname: Kalff
authors.affiliation: Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany; Center for Integrated Oncology Cologne/Bonn, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany. Electronic address: ines.guetgemann@ukb.uni-bonn.de.
authors.forename: Ines
authors.initials: I
authors.lastname: Gütgemann
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005784:Gene Amplification; D057166:High-Throughput Screening Assays; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: C496348:FGFR1 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1
keywords: Amplification; FGFR1; FISH; Fibroblast growth factor receptor 1; Fluorescence in situ hybridization; Gastric adenocarcinoma; Gastric cancer