title: Fish oil-supplemented parenteral nutrition could alleviate acute lung injury, modulate immunity, and reduce inflammation in rats with abdominal sepsis.
abstract: The objectives were to confirm that intravenous fish oil (FO) emulsions could alleviate acute lung injury, modulate immunity, and reduce inflammation in rats with abdominal sepsis and to explore the mechanisms of these effects. Thirty-six adult male Sprague-Dawley rats were divided into 4 groups randomly. Two days after central venous catheterization, rats were subjected to cecal ligation and puncture to produce abdominal sepsis. Rats were assigned to receive normal saline or total parenteral nutrition (TPN) containing standard soybean oil emulsions or FO-supplemented TPN at the onset of sepsis for 5 days. A sham operation and control treatment were performed in control group rats. Acute lung injury scores, peripheral blood lymphocyte subsets, plasma cytokines, and Foxp3 expression in the spleen were determined. Compared with the normal saline and TPN without FO, FO-supplemented TPN beneficially altered the distributions of the T-lymphocyte subsets and downregulated the acute lung injury scores, plasma cytokines, and expression of Foxp3 due to sepsis. Fish oil-supplemented TPN can decrease acute lung injury scores, alleviate histopathology, reduce the bacterial load in the peritoneal lavage fluid, modulate the lymphocyte subpopulation in the peripheral blood, downregulate Foxp3 expression in the spleen, and reduce plasma cytokines, which means that FO-supplemented TPN can alleviate acute lung injury, modulate immunity, and reduce inflammation in rats with abdominal sepsis.
authors.affiliation: Department of Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Xianxiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Enqin
authors.initials: E
authors.lastname: Yang
authors.affiliation: Laboratory Animal Center of Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Nanyang
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address: briancao@126.com.
authors.forename: Shougen
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address: zhouyanbing999@aliyun.com.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000005:Abdomen / Q000382:microbiology; D055371:Acute Lung Injury / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D002432:Cecum / Q000293:injuries; D016207:Cytokines / Q000097:blood; D019587:Dietary Supplements; D005395:Fish Oils / Q000494:pharmacology / Q000627:therapeutic use*; D051858:Forkhead Transcription Factors / Q000378:metabolism; D007109:Immunity / Q000187:drug effects*; D007249:Inflammation / Q000097:blood / Q000188:drug therapy*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D016131:Lymphocyte Subsets / Q000378:metabolism; D008297:Male; D010289:Parenteral Nutrition, Total / Q000379:methods*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood / Q000150:complications / Q000276:immunology / Q000628:therapy*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
chemical_list: D016207:Cytokines; D005395:Fish Oils; D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat
keywords: ALI; Cytokines; Fish oil; Immune function; Parenteral nutrition; Sepsis; T-lymphocyte subsets