title: Expression profile of ADAM10 and ADAM17 in allergic rhinitis.
abstract: BACKGROUND
  A disintegrin and metalloprotease (ADAM) is a multifunctional gene family that contributes to the homeostasis of the extracellular matrix, transduction of specific intracellular signals, organogenesis, inflammation, tissue remodeling, adhesion, and cell migration. ADAM17 is the best characterized sheddase, with widespread putative substrates, including various inflammatory modulators. ADAM10 is the most similar ADAM to ADAM17 in terms of both protein sequence and the structural properties of their catalytic domains. The objective of this work was to assess the expression of ADAM10 and ADAM17 in allergic rhinitis to gain insight into their respective roles.
  
  
  METHODS
  The expression of ADAM10 and ADAM17 was investigated in the nasal mucosa under allergic and nonallergic conditions. Tissue samples were evaluated by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting, and data were analyzed semiquantitatively with densitometry.
  
  
  RESULTS
  The ADAM17 messenger RNA (mRNA) level was significantly (p < 0.001) lower in the allergic nasal mucosa than in the nonallergic nasal mucosa, whereas the ADAM10 mRNA level was significantly (p < 0.001) lower in the nonallergic nasal mucosa. The ADAM17 protein levels were also significantly (p < 0.001) lower in the allergic nasal mucosa, whereas the ADAM10 protein levels were lower in the nonallergic nasal mucosa (p = 0.002).
  
  
  CONCLUSIONS
  Decreased expression of ADAM17 and increased expression of ADAM10 may contribute to the development of allergic rhinitis through unknown pathways. We suggest that understanding the expression profile of ADAM17 and ADAM10 might help to elucidate the mechanism of allergic rhinitis.
authors.affiliation: Department of Pediatrics, Dong-A University, College of Medicine, Busan, Korea.
authors.forename: Hee Won
authors.initials: HW
authors.lastname: Chueh
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Inje University, College of Medicine, Busan Paik Hospital, Busan, Korea.
authors.forename: Seong Kook
authors.initials: SK
authors.lastname: Park
authors.affiliation: Department of Anatomy and Research Center for Tumor Immunology, Inje University, College of Medicine, Busan Paik Hospital, Busan, Korea.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Hur
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Dong-A University, College of Medicine, Busan, Korea.
authors.forename: Woo Yong
authors.initials: WY
authors.lastname: Bae
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000072197:ADAM10 Protein; D000072198:ADAM17 Protein; D053829:Amyloid Precursor Protein Secretases / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000502:physiology*; D065631:Rhinitis, Allergic / Q000276:immunology*
chemical_list: D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human
keywords: ADAM10; ADAM17; Western blotting; allergic rhinitis; inferior turbinate; reverse-transcription polymerase chain reaction