title: Polypyrrole/magnetic nanoparticles composite as an efficient sorbent for dispersive micro-solid-phase extraction of antidepressant drugs from biological fluids.
abstract: In this study, polypyrrole/magnetic nanoparticles composites in the presence of two different dopants were synthesized with the aid of chemical oxidative polymerization process for dispersive-μ-solid phase extraction (D-μ-SPE). The synthesized magnetic sorbents were characterized by various techniques. The results exhibited that the nanocomposite modified by polypyrrole with sodium perchlorate as a dopant demonstrated higher extraction efficiency for citalopram (CIT) and sertraline (STR) as the model compounds. This nanosorbent in combination with high performance liquid chromatography-UV detection was applied for extraction, preconcentration and determination of CIT and STR in urine and plasma samples. The effect of various parameters on the extraction efficiency including: sample pH, amount of sorbent, sorption time, eluent and its volume, salt content, and elution time were investigated and optimized. The opted conditions were: sample pH, 9.0; sorbent dosage, 10mg; sorption time, 7 min; elution solvent and its volume, 0.06 mol L(-1) HCl in methanol, 120 μL; elution time, 2 min and without addition of salt to the sample. The calibration curves were linear in the concentration range of 1-800 μg L(-1). The limits of detection (LODs) were obtained in the range of 0.2-1.0 μg L(-1) for CIT and 0.3-0.7 μg L(-1) for STR, respectively. The percent of extraction recoveries and relative standard deviations (n=5) were in the range of 93.4-99, 4.8-8.4 for CIT and 94-98.4, 4.3-9.2 for STR, respectively. Finally, the applicability of the method was successfully confirmed by the extraction and determination of CIT and STR in human urine and plasma samples.
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Asgharinezhad
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Karami
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran. Electronic address: h-ebrahim@sbu.ac.ir.
authors.forename: Homeira
authors.initials: H
authors.lastname: Ebrahimzadeh
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Shekari
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Niloofar
authors.initials: N
authors.lastname: Jalilian
mesh_terms: D000327:Adsorption; D000928:Antidepressive Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008280:Magnetics / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D000928:Antidepressive Agents; D058185:Magnetite Nanoparticles; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole
keywords: Biological fluids; Citalopram; Dispersive-μ-solid phase extraction; Fe(3)O(4)@polypyrrole nanocomposite; Sertraline