title: Removal of rRNA from Deproteinized, Phenol-Extracted Total RNA by Hybrid Selection.
abstract: In this protocol, rRNAs are selectively removed from a total RNA sample by hybridizing the rRNAs to complementary biotinylated oligodeoxynucleotides that can be affinity-purified using streptavidin beads, leaving all other RNAs behind. Although commercially available kits can be used to perform this procedure, they are expensive. We recommend that investigators order species-specific oligodeoxynucleotides for their own applications. There are well-established secondary structure predictions for all rRNAs.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Nilsen
mesh_terms: D019921:Biotinylation; D002846:Chromatography, Affinity / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification*; D019809:Streptavidin / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D012335:RNA, Ribosomal; D019809:Streptavidin