title: Preparation, Characterization, and Pharmacological Activity of Cymbopogon winterianus Jowitt ex Bor (Poaceae) Leaf Essential Oil of β-Cyclodextrin Inclusion Complexes.
abstract: This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in β-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in β-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50-200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamate-induced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p < 0.05) reduced. The number of Fos-positive cells was significantly changed in the dorsal raphe nucleus (p < 0.01), locus coeruleus (p < 0.001), trigeminal nucleus (p < 0.05), and trigeminal thalamic tract (p < 0.05). LEO-CD did not cause changes in motor coordination in the rota-rod test. Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Priscila L
authors.initials: PL
authors.lastname: Santos
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Adriano A S
authors.initials: AA
authors.lastname: Araújo
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Jullyana S S
authors.initials: JS
authors.lastname: Quintans
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Makson G B
authors.initials: MG
authors.lastname: Oliveira
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Renan G
authors.initials: RG
authors.lastname: Brito
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Mairim R
authors.initials: MR
authors.lastname: Serafini
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Paula P
authors.initials: PP
authors.lastname: Menezes
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Marcio R V
authors.initials: MR
authors.lastname: Santos
authors.affiliation: Department of Chemistry, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Pericles B
authors.initials: PB
authors.lastname: Alves
authors.affiliation: Department of Morphology, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Waldecy
authors.initials: W
authors.lastname: de Lucca Júnior
authors.affiliation: Department of Agronomy, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Arie F
authors.initials: AF
authors.lastname: Blank
authors.affiliation: Postgraduate Program in Natural Products and Bioactive Synthetics (PgPNSB), Federal University of Paraíba, 58051-900 João Pessoa, PB, Brazil.
authors.forename: Viviana
authors.initials: V
authors.lastname: La Rocca
authors.affiliation: Postgraduate Program in Natural Products and Bioactive Synthetics (PgPNSB), Federal University of Paraíba, 58051-900 João Pessoa, PB, Brazil.
authors.forename: Reinaldo N
authors.initials: RN
authors.lastname: Almeida
authors.affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quintans-Júnior