title: Antitumor and immunomodulatory activities of a water-soluble polysaccharide from Chaenomeles speciosa.
abstract: In this study, a water-soluble polysaccharide (CSP) was successfully purified from Chaenomeles speciosa by DEAE-Sepharose and Sephadex G-100 column chromatography. CSP had a weight-average molecular weight of about 6.3 × 10(4)Da and was composed of glucose (Glc), galactose (Gal), rhamnose (Rha) and arabinose (Ara) with a relative molar ratio of 4.6:1.3:0.8:0.5. CSP could not only inhibit the growth of S180 tumor transplanted in mice, but also increase the relative spleen index and body weight of tumor bearing mice. Moreover, concanavalin A (ConA) and lipopolysaccharide (LPS) induced splenocyte proliferation and peritoneal macrophage phagocytosis were also enhanced after CSP administration. Furthermore, CSP treatment could improve delayed type hypersensitivity (DTH) and promote the secretion of IL-2, TNF-α and IFN-γ in serum. The overall findings suggest that the antitumor effect of CSP is might be associated with its potent immunostimulatory activity.
authors.affiliation: Department of Pharmacology, Basic Medical School, Wuhan University, Wuhan 430072, PR China; College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China. Electronic address: Xiexianfeiut@gmail.com.
authors.forename: Xianfei
authors.initials: X
authors.lastname: Xie
authors.affiliation: College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Zou
authors.affiliation: College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chenghai
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood / Q000276:immunology; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D027824:Rosaceae / Q000737:chemistry*; D012510:Sarcoma 180 / Q000097:blood / Q000188:drug therapy / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007155:Immunologic Factors; D007376:Interleukin-2; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: Antitumor; Chaenomeles speciosa; Immunomodulation; Polysaccharides