title: Anti-inflammatory properties and expression in selected organs of canine interleukin-1β splice variant 1.
abstract: The IL-1β gene can be also be spliced with the intron 4 retention; the result is a IL-1β splice variant 1 (IL-1βsv1), which was significantly up-regulated in failing myocardium of dogs suffering from chronic degenerative valvular disease (CDVD). Expression of IL-1βsv1 was assessed, at both RNA and protein levels, in organs affected by heart failure, namely, kidneys, liver, and lungs from 35 dogs suffering chronic degenerative valvular disease (CDVD) and in 20 disease free control dogs. IL-1βsv1 RNA was detected in the dogs from both groups. In the CDVD group, the highest RNA and protein IL-1βsv1 levels were observed in lungs, followed, in that order, by the liver and kidneys. IL-1βsv1 protein was found in the cytoplasm of hepatocytes and IL-1βsv1-overexpressing DH82 cells. In lungs, IL-1βsv1 was localized in the cytoplasm and in the nuclei of bronchiolar epithelial and smooth-muscle cells. Cytoplasmic and nuclear IL-1βsv1 expression was observed in macrophages, and a strong nuclear signal was detected in epithelial cells of the alveolar sacs. Following lipopolysaccharide (LPS) stimulation, overexpression of IL-1βsv1 in DH82 cells decreased the pro-inflammatory response. Our results indicate that IL-1βsv1 is constitutively expressed in both normal tissues and in tissues from cases of heart failure. The presence of IL-1βsv1 in tissues exposed to invading agents and its anti-inflammatory activity in DH82 cells may point to its immunomodulatory role in vivo.
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: liliana.kiczak@up.wroc.pl.
authors.forename: L
authors.initials: L
authors.lastname: Kiczak
authors.affiliation: Department of Food Hygiene and Consumer Protection, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Wałecka-Zacharska
authors.affiliation: Department of Food Hygiene and Consumer Protection, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Bania
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Sambor
authors.affiliation: Department of Immunology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Stefaniak
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Dzięgiel
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zacharski
authors.affiliation: Department of Heart Diseases, Wroclaw Medical University, Wroclaw, Poland; Department of Internal Diseases with Clinic for Horses, Dogs, and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Tomaszek
authors.affiliation: Department of Heart Diseases, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Rybińska
authors.affiliation: Department of Internal Diseases with Clinic for Horses, Dogs, and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: U
authors.initials: U
authors.lastname: Pasławska
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology; D004285:Dogs / Q000235:genetics* / Q000276:immunology*; D015536:Down-Regulation; D015870:Gene Expression; D006333:Heart Failure / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D006349:Heart Valve Diseases / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D006706:Homeostasis / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics*; D008070:Lipopolysaccharides / Q000008:administration & dosage; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics; D015398:Signal Transduction / Q000276:immunology; D014162:Transfection
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D020033:Protein Isoforms
keywords: Alternative splicing; Dog; Inflamation; Interleukin-1β; Liver; Lung