title: Vitroprocines, new antibiotics against Acinetobacter baumannii, discovered from marine Vibrio sp. QWI-06 using mass-spectrometry-based metabolomics approach.
abstract: A robust and convenient research strategy integrating state-of-the-art analytical techniques is needed to efficiently discover novel compounds from marine microbial resources. In this study, we identified a series of amino-polyketide derivatives, vitroprocines A-J, from the marine bacterium Vibrio sp. QWI-06 by an integrated approach using imaging mass spectroscopy and molecular networking, as well as conventional bioactivity-guided fractionation and isolation. The structure-activity relationship of vitroprocines against Acinetobacter baumannii is proposed. In addition, feeding experiments with (13)C-labeled precursors indicated that a pyridoxal 5'-phosphate-dependent mechanism is involved in the biosynthesis of vitroprocines. Elucidation of amino-polyketide derivatives from a species of marine bacteria for the first time demonstrates the potential of this integrated metabolomics approach to uncover marine bacterial biodiversity.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chih-Chuang
authors.initials: CC
authors.lastname: Liaw
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Pei-Chin
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Chao-Jen
authors.initials: CJ
authors.lastname: Shih
authors.affiliation: Department of Medical Laboratory Sciences and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Sung-Pin
authors.initials: SP
authors.lastname: Tseng
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Ying-Mi
authors.initials: YM
authors.lastname: Lai
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Chi-Hsin
authors.initials: CH
authors.lastname: Hsu
authors.affiliation: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093-0636, United States.
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Dorrestein
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.|Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Yang
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000254:growth & development; D000605:Amino Alcohols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D059001:Aquatic Organisms; D002247:Carbon Isotopes; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods; D008826:Microbial Sensitivity Tests; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011732:Pyridoxal Phosphate / Q000378:metabolism; D013329:Structure-Activity Relationship; D014733:Vibrio / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000605:Amino Alcohols; D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D061065:Polyketides; D011732:Pyridoxal Phosphate