title: Gadolinium-complexed Aβ-binding contrast agents for MRI diagnosis of Alzheimer's Disease.
abstract: MRI contrast agents, containing peptide sequences that bind β-amyloid and gadolinium ions ligated to DOTA have been synthesized for evaluation in early diagnosis of Alzheimer's Disease in transgenic mice models. A number of brain penetration modifications were incorporated and sufficient amounts of contrast agent in the brain were achieved only by addition of a cationic cell penetration sequence along with the use of microparticle assisted ultrasound activation. In the T1 mode of a MRI scan, the peptide (R2) illuminated areas of brain rich in amyloid plaques.
authors.affiliation: Dept of Basic Medical Sciences, St George's University of London, Cranmer Terrace, London SW17 ORE, United Kingdom.
authors.forename: Balpreet
authors.initials: B
authors.lastname: Matharu
authors.affiliation: Dept of Basic Medical Sciences, St George's University of London, Cranmer Terrace, London SW17 ORE, United Kingdom.
authors.forename: Nick
authors.initials: N
authors.lastname: Spencer
authors.affiliation: Dept of Cardiovascular Science, St George's University of London, Cranmer Terrace, London SW17 ORE, United Kingdom.
authors.forename: Franklyn
authors.initials: F
authors.lastname: Howe
authors.affiliation: Dept of Basic Medical Sciences, St George's University of London, Cranmer Terrace, London SW17 ORE, United Kingdom. Electronic address: sghk200@sgul.ac.uk.
authors.forename: Brian
authors.initials: B
authors.lastname: Austen
mesh_terms: D000328:Adult; D000544:Alzheimer Disease / Q000175:diagnosis* / Q000000981:diagnostic imaging; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000473:pathology; D003287:Contrast Media / Q000493:pharmacokinetics; D005682:Gadolinium / Q000493:pharmacokinetics; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000737:chemistry; D058225:Plaque, Amyloid / Q000473:pathology; D049268:Positron-Emission Tomography; D011868:Radioisotopes; D014018:Tissue Distribution; D055815:Young Adult
chemical_list: D016229:Amyloid beta-Peptides; D003287:Contrast Media; D010446:Peptide Fragments; D011868:Radioisotopes; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); D005682:Gadolinium
keywords: Alzheimer's; Contrast; MRI; Transgenic; β-Amyloid