title: Yes-associated protein (YAP) expression is involved in epithelial-mesenchymal transition in hepatocellular carcinoma.
abstract: OBJECTIVE
  To investigate biological impact of the downregulation of yes-associated protein (YAP) through RNA interference in the process of epithelial-mesenchymal transition in MHCC97H and MHCC97L.
  
  
  METHODS
  MHCC97H and MHCC97L cells were transiently transfected by YAP-siRNA. Furthermore, protein expressions and mRNA levels of characteristic markers of epithelial-mesenchymal transition (E-cadherin, N-cadherin) were examined by Western blotting and real-time polymerase chain reaction, and transwell invasion assay was used to detect changes of invasiveness of MHCC97H and MHCC97L cells.
  
  
  RESULTS
  The transfected group with YAP-siRNA in MHCC97H after 72 h by Western blotting showed obviously higher expression of E-cadherin compared with the control group (P < 0.05), and lower expression of N-cadherin (P < 0.05). In MHCC97L cells, the expression of E-cadherin was also significantly increased (P < 0.05); however, N-cadherin expression did not significantly change (P > 0.05). Moreover, compared with the control group, Transwell invasion assay showed that the number of the transfected groups was significantly decreased in MHCC97H and MHCC97L cell lines (both P < 0.05). The result of real-time polymerase chain reaction indicated that mRNA levels of E-cadherin increased (P < 0.05), but the mRNA levels of N-cadherin did not significantly change (P > 0.05) in these two cell lines, indicating some effects of post-transcriptional regulation mechanism after silencing YAP.
  
  
  CONCLUSIONS
  YAP expression in human hepatocellular carcinoma cell lines MHCC97H and MHCC97L is closely related with the characteristic markers of epithelial-mesenchymal transition, N-cadherin and E-cadherin expression.
authors.affiliation: Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China.|Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200000, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China.
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China.
authors.forename: B
authors.initials: B
authors.lastname: Fu
authors.affiliation: Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200000, China.
authors.forename: M
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200000, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Quan
authors.affiliation: Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China. chenguihua1955@126.com.
authors.forename: G
authors.initials: G
authors.lastname: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors; D014162:Transfection; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human
keywords: Epithelial–mesenchymal transition (EMT); Hepatocellular carcinoma (HCC); YAP (yes-associated protein)