title: Developmental exposure to paracetamol causes biochemical alterations in medulla oblongata.
abstract: The effect and safety of prenatal and early life administration of paracetamol - routinely used over-the-counter antipyretic and analgesic medication on monoamines content and balance of amino acids in the medulla oblongata is still unknown. In this study we have determined the level of neurotransmitters in this structure in two-month old Wistar male rats exposed to paracetamol in the dose of 5 (P5, n=10) or 15mg/kg b.w. (P15, n=10) during prenatal period, lactation and till the end of the second month of life. Control group received drinking water (Con, n=10). Monoamines, their metabolites and amino acids concentration in medulla oblongata of rats were determined using high performance liquid chromatography (HPLC) in 60 postnatal day (PND60). This experiment shows that prenatal and early life paracetamol exposure modulates neurotransmission associated with serotonergic, noradrenergic and dopaminergic system in medulla oblongata. Reduction of alanine and taurine levels has also been established.
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Blecharz-Klin
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Joniec-Maciejak
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jawna
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Pyrzanowska
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piechal
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Adriana
authors.initials: A
authors.lastname: Wawer
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland. Electronic address: etyszkiewicz@wum.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Widy-Tyszkiewicz
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015306:Biogenic Monoamines / Q000378:metabolism*; D002853:Chromatography, Liquid; D005260:Female; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D008526:Medulla Oblongata / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D015306:Biogenic Monoamines; D000082:Acetaminophen
keywords: Acetaminophen; Amino acids; CNS; Medulla oblongata; Neurotransmission; Paracetamol