title: Study on the release routes of allelochemicals from Pistia stratiotes Linn., and its anti-cyanobacteria mechanisms on Microcystis aeruginosa.
abstract: Allelochemicals in Pistia stratiotes Linn. have a strong anti-cyanobacteria effect on Microcystis aeruginosa. To further determine the release routes of allelochemicals in P. stratiotes and understand their anti-cyanobacteria mechanisms, we aimed to systematically investigate the allelopathic effects of leaf leachates, leaf volatilization, root exudates, and residue decomposition of P. stratiotes on M. aeruginosa. The influences of P. stratiotes allelochemicals on the physiological properties of M. aeruginosa were also studied. Root exudates of P. stratiotes exhibited the strongest inhibitory effect on M. aeruginosa growth. The residue decomposition and leaf leachates exhibited a relatively strong inhibitory effect on M. aeruginosa growth. By contrast, the leaf volatilization stimulated M. aeruginosa growth. Therefore, root exudation was determined to be the main release route of allelochemicals from P. stratiotes. The mixed culture experiment of P. stratiotes root exudates and M. aeruginosa showed that the allelochemicals released from root exudation had no effect on the electron transfer of M. aeruginosa photosynthetic system II. However, it reduced the phycocyanin (PC) content and phycocyanin to allophycocyanin (PC/APC) ratio in the photosynthetic system. As the root exudates concentration increased, the electrical conductivity (EC) and superoxide anion radical (O2(*-)) values in the M. aeruginosa culture fluid increased significantly, indicating that the allelochemicals released from the root of P. stratiotes inhibited algae growth by affecting the PC and PC/APC levels in photosynthesis, destroying the cell membrane, and increasing O2(*-) content to result in oxidative damage of M. aeruginosa.
authors.affiliation: Key Laboratory of Aquatic Resources Conservation and Development Technology Research, College of Life Sciences, Huzhou University, Huzhou, Zhejiang, 313000, China. wwxx1981@126.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Environmental Protection Monitoring Centre Station, Huzhou, Zhejiang, 313000, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Aquatic Resources Conservation and Development Technology Research, College of Life Sciences, Huzhou University, Huzhou, Zhejiang, 313000, China.
authors.forename: Jinyun
authors.initials: J
authors.lastname: Ye
authors.affiliation: Key Laboratory of Aquatic Resources Conservation and Development Technology Research, College of Life Sciences, Huzhou University, Huzhou, Zhejiang, 313000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhong
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis; D029064:Araceae / Q000737:chemistry / Q000633:toxicity*; D000458:Cyanobacteria; D004553:Electric Conductivity; D046931:Microcystis / Q000737:chemistry / Q000187:drug effects*; D010675:Pheromones / Q000032:analysis / Q000633:toxicity*; D010798:Phycocyanin / Q000032:analysis; D053147:Plant Exudates / Q000737:chemistry / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D028321:Plant Preparations / Q000633:toxicity*; D013481:Superoxides / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D010675:Pheromones; D053147:Plant Exudates; D028321:Plant Preparations; C002497:allophycocyanin; D010798:Phycocyanin; D013481:Superoxides
keywords: Allelochemical; Anti-cyanobacteria mechanism; Microcystis aeruginosa; Pistia stratiotes Linn.; Release route