CHEMICAL COMPOSITION, CHARACTERIZATION OF ANTHOCYANINS AND ANTIOXIDANT POTENTIAL OF EUTERPE EDULIS FRUITS: APPLICABILITY ON GENETIC DYSLIPIDEMIA AND HEPATIC STEATOSIS IN MICE.
The significance of polyphenol intake for the prevention of chronic diseases is controversial.


OBJECTIVE
this study investigated the chemical composition and antioxidant potential of an anthocyanin-rich extract from Euterpe edulis fruits (LPEF) and its effects on liver steatosis in dyslipidemic apoE-/- knockout mice.


METHODS
mice were divided into G1 (C57BL/6) standard diet; G2 (apoE-/-) standard diet, G3 (apoE-/-) 2% LPEF, G4 (apoE-/-) 6% LPEF, G5 (apoE-/-) 10% LPEF, G6 (apoE-/-) 2% α-tocopherol acetate. After 75 days of treatment, the animals were euthanized. The LPEF contained a high level of monomeric anthocyanins (301.4 mg/100g) and marked antioxidant activity.


RESULTS
Catalase activity was reduced in G3, G4, G5 and G6 compared to G2. Superoxide dismutase was reduced only in G4. The animals in G4, G5, and G6 showed low HDL and triglycerides levels compared to G2. The proportion of lipid droplets in liver tissue was reduced in G4 and G5 compared to G2, G3, and G6.


CONCLUSIONS
The results indicated that E. edulis pulp is rich in anthocyanins and the LPEF dietary consumption can reduce the severity of liver steatosis in apoE-/- mice, an effect that is potentially mediated by the antioxidant activity of this extract and modulation of triglyceride serum levels.
Department of Biochemistry and Molecular Biology, Federal University of Viçosa.. marques@yahoo.com.br.
Luciana
L
Marques Cardoso
Department of Cell, Tissue and Developmental Biology, Federal University of Alfenas.. romuonovaes@yahoo.com.br.
Rômulo
R
Dias Novaes
Department of Nutrition and Health, Federal University of Viçosa.. acastro@ufv.br.
Cynthia
C
Aparecida de Castro
Department of Nutrition and Health, Federal University of Viçosa.. Novello@yahoo.com.br.
Alexandre
A
Azevedo Novello
Department of Animal Biology, Federal University of Viçosa.. reggysvilela@yahoo.com.br.
Reggiani
R
Vilela Gonçalves
Department of Vegetal Biology, Federal University of Viçosa, Brazil.. Ricci-Silva@ufv.br.
Maria Esther
ME
Ricci-Silva
Department of Biochemistry and Molecular Biology, Federal University of Viçosa.. hramos@yahoo.com.br.
Humberto Josué
HJ
de Oliveira Ramos
Department of Nutrition and Health, Federal University of Viçosa.. mpeluzio@ufv.br.
Maria do Carmo
Mdo C
Gouveia Peluzio
Department of Biochemistry and Molecular Biology, Federal University of Viçosa.. jpvleite@ufv.br.
João Paulo
JP
Viana Leite
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001057:Apolipoproteins E / Q000172:deficiency; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D002149:Energy Intake; D065748:Euterpe / Q000737:chemistry*; D005234:Fatty Liver / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005638:Fruit / Q000737:chemistry*; D008055:Lipids / Q000097:blood; D051379:Mice; D018345:Mice, Knockout; D010936:Plant Extracts / Q000737:chemistry*
D000872:Anthocyanins; D000975:Antioxidants; D001057:Apolipoproteins E; D008055:Lipids; D010936:Plant Extracts