Dynamics of blood viscosity regulation during hypoxic challenges in the chicken embryo (Gallus gallus domesticus).
Hypoxia in chicken embryos increases hematocrit (Hct), blood O2 content, and blood viscosity. The latter may limit O2 transport capacity (OTC) via increased peripheral resistance. Hct increase may result from increased nucleated red blood cell concentration ([RBC]) and mean corpuscular volume (MCV) or reduced plasma volume. We hypothesized changes in Hct, hemoglobin concentration ([Hb]), [RBC] and MCV and their effects on viscosity would reduce OTC. Five experimental treatments that increase Hct were conducted on day 15 embryos: 60min water submergence with 60min recovery in air; exposure to 15% O2 with or without 5% CO2 for 24 h with 6 h recovery; or exposure to 10% O2 with or without 5% CO2 for 120 min with 120 min recovery. Control Hct, [Hb], [RBC], MCV, and viscosity were approximately 26%, 9g%, 2.0 10(6)μL(-1), 130μm(3), and 1.6mPas, respectively. All manipulations increased Hct and blood viscosity without changing blood osmolality (276mmolkg(-1)). Increased viscosity was attributed to increased [RBC] and MCV in submerged embryos, but solely MCV in embryos experiencing 10% O2 regardless of CO2. Blood viscosity in embryos exposed to 15% O2 increased via increased MCV alone, and viscosity was constant during recovery despite increased [RBC]. Consequently, blood viscosity was governed by MCV and [RBC] during submergence, while MCV was the strongest determinant of blood viscosity in extrinsic hypoxia with or without hypercapnia. Increased Hct and blood O2 content did not compensate for the effect of increased viscosity on OTC during these challenges.
Developmental and Integrative Biology, Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX 76203, USA. Electronic address: zac.f.kohl@gmail.com.
Zachary F
ZF
Kohl
Developmental and Integrative Biology, Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX 76203, USA.
Dane A
DA
Crossley
Developmental and Integrative Biology, Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX 76203, USA.
Hiroshi
H
Tazawa
Developmental and Integrative Biology, Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX 76203, USA.
Warren W
WW
Burggren
D000818:Animals; D017849:Animals, Inbred Strains; D001809:Blood Viscosity; D002642:Chick Embryo; D004906:Erythrocyte Count / Q000662:veterinary; D004909:Erythrocyte Indices / Q000662:veterinary; D006400:Hematocrit / Q000662:veterinary; D006935:Hypercapnia / Q000196:embryology / Q000209:etiology / Q000662:veterinary; D000860:Hypoxia / Q000097:blood / Q000196:embryology / Q000503:physiopathology / Q000662:veterinary*; D007101:Immersion / Q000009:adverse effects; D010100:Oxygen / Q000097:blood; D011201:Poultry Diseases / Q000097:blood / Q000196:embryology* / Q000209:etiology / Q000503:physiopathology; D011897:Random Allocation; D012720:Severity of Illness Index; D015854:Up-Regulation; D014882:Water-Electrolyte Balance
D010100:Oxygen
Blood viscosity; Cardiovascular; Chick embryo; Chicken; Hematocrit; Hypercapnia; Hypoxia; Optimal hematocrit; Oxygen transport; Stress