Targeted grafting of thermoresponsive polymers from a penetrative honeycomb structure for cell sheet engineering.
Responsive membranes have been used to construct smart biomaterial interfaces. We report a novel approach to fabricate honeycomb films with a pattern of thermoresponsive polymer, namely poly(N-isopropylacrylamide). The approach was based on a combination of the breath figure method and reversible addition-fragmentation chain transfer. The hybrid film had morphological and chemical patterns resulting in varied wettability and morphology at various stages, as well as high thermo-responsiveness. Enhanced cell adhesion was observed at an incubation temperature of 37 °C, which is above its lower critical solution temperature (LCST). Furthermore, cells could be harvested at temperatures below the LCST without trypsin treatment. The non-invasive characteristics give this membrane potential as a substrate for cell sheet engineering.
School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai, 200240, China. qhlu@sjtu.edu.cn xueminlu@sjtu.edu.cn.
Shuangshuang
S
Chen
Xuemin
X
Lu
Dandan
D
Zhu
Qinghua
Q
Lu
D000178:Acrylamides / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*; D017155:Wettability
D000178:Acrylamides; D011108:Polymers; C067295:N-isopropylacrylamide