A high-density SSR genetic map constructed from a F2 population of Gossypium hirsutum and Gossypium darwinii.
The cultivated allotetraploid species Gossypium hirsutum, accounts for 90% of the world cotton production, has narrow genetic basis that's why its yield, quality or stress resistance breeding is stagnant. It is therefore, essential to explore desirable genes from Gossypium darwinii which has enviable traits such as high fiber fineness, drought tolerance, fusarium and verticillium resistance. We used G. darwinii as primary plant materials in this study not only to enrich the genetic diversity of exiting germplasm but also to better understand its genome structure. An interspecific high density linkage map of allotetraploid cotton was constructed using F2 population (G. hirsutum×G. darwinii). The map was based entirely on genome-wide simple sequence repeat (SSR) markers. A total of 2763 markers were mapped in 26 linkage groups (chromosomes) covering a genome length of 4176.7cM with an average inter-locus distance of 1.5cM. The length of the chromosomes ranged from 84.7 to 238.5cM with an average length of 160.6cM. At subgenome length was 2160.7cM with an average distance of 1.6cM, where as Dt genome length was 2016cM with an average distance of 1.4cM. There were 601 distorted SSR loci. Less number of segregation distortion loci were located in At subgenome than in Dt subgenome. Two post-polyploidization reciprocal translocations of "A2/A3 and A4/A5" were suggested by 44 pairs of duplicate loci.
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; Cotton Sciences Research Institute of Hunan/National Hybrid Cotton Research Promotion Center, Changde, Hunan 415101, China. Electronic address: chdmks@163.com.
Haodong
H
Chen
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; Nuclear Institute for Agriculture & Biology (NIAB), Faisalabad, Pakistan. Electronic address: mkrkhan@gmail.com.
M Kashif Riaz
MK
Khan
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: zhonglizhou@163.com.
Zhongli
Z
Zhou
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: wxx1991@126.com.
Xingxing
X
Wang
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: kxncai0611@163.com.
Xiaoyan
X
Cai
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; National Agricultural Research Centre, Park Road, Islamabad, Pakistan. Electronic address: kashifnarc@gmail.com.
M Kashif
MK
Ilyas
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: wcy2525377@126.com.
Chunying
C
Wang
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: wangyh2525377@163.com.
Yuhong
Y
Wang
Cotton Sciences Research Institute of Hunan/National Hybrid Cotton Research Promotion Center, Changde, Hunan 415101, China. Electronic address: lizhong863@sina.com.
Yuqiang
Y
Li
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: liufcri@163.com.
Fang
F
Liu
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: wkbcri@163.com.
Kunbo
K
Wang
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D020440:Gene Duplication; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic
D005819:Genetic Markers
Chromosome mapping; Cotton (Gossypium spp.); Genomics; Segregation distortion