S100P regulates trophoblast-like cell proliferation via P38 MAPK pathway.
S100P was originally isolated from the placenta, and is expressed in very high levels in trophoblast cells, but its role on trophoblast cells proliferation has not yet been studied. In this study, we aimed to investigate the potential role of S100P in human placental development, and the impact of its expression regulation on cellular function as well as molecular mechanisms involved in trophoblast-like cells. We found that the expression of S100P in first trimester placenta was significantly reduced in spontaneous abortion patients with respect to normal pregnant women. Up-regulation of S100P in JAR cells promoted JAR cells proliferation, and increased the expression of phosphorylated P38 (p-P38) mitogen-activated protein kinase (MAPK) and p-ERK MAPK. However, the effects of S100P on JAR cells proliferation were prevented by P38 inhibitor-SB203580, but not by ERK inhibitor-PD98059. These results showed that S100P may have a physiological role in normal pregnant development, and regulate trophoblast-like cell proliferation via modulating the P38 MAPK pathway.
a Assisted Reproduction Unit, Department of Obstetrics and Gynecology , Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University , Hangzhou , China.
Hai-Yan
HY
Zhu
a Assisted Reproduction Unit, Department of Obstetrics and Gynecology , Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University , Hangzhou , China.
Jun-Xia
JX
Wang
a Assisted Reproduction Unit, Department of Obstetrics and Gynecology , Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University , Hangzhou , China.
Xiao-Mei
XM
Tong
a Assisted Reproduction Unit, Department of Obstetrics and Gynecology , Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University , Hangzhou , China.
Ya-Mei
YM
Xue
a Assisted Reproduction Unit, Department of Obstetrics and Gynecology , Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University , Hangzhou , China.
Song-Ying
SY
Zhang
D000022:Abortion, Spontaneous / Q000378:metabolism; D000328:Adult; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010766:Phosphorylation; D010929:Placentation / Q000502:physiology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D015398:Signal Transduction / Q000502:physiology; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; C103003:S100P protein, human; D048051:p38 Mitogen-Activated Protein Kinases
Proliferation; S100P; trophoblast cell