Using gastric juice lncRNA-ABHD11-AS1 as a novel type of biomarker in the screening of gastric cancer.
Long noncoding RNAs (lncRNAs) play vital roles in tumorigenesis. However, the diagnostic values of most lncRNAs are largely unknown. To investigate whether gastric juice lncRNA-ABHD11-AS1 can be a potential biomarker in the screening of gastric cancer, 173 tissue samples and 130 gastric juice from benign lesion, gastric dysplasia, gastric premalignant lesions, and gastric cancer were collected. ABHD11-AS1 levels were detected by reverse transcription-polymerase chain reaction. Then, the relationships between ABHD11-AS1 levels and clinicopathological factors of patients with gastric cancer were investigated. The results showed that ABHD11-AS1 levels in gastric cancer tissues were significantly higher than those in other tissues. Its levels in gastric juice from gastric cancer patients were not only significantly higher than those from cases of normal mucosa or minimal gastritis, atrophic gastritis, and gastric ulcers but also associated with gender, tumor size, tumor stage, Lauren type, and blood carcinoembryonic antigen (CEA) levels. More importantly, when using gastric juice ABHD11-AS1 as a marker, the positive detection rate of early gastric cancer patients was reached to 71.4 %. Thanks to the special origin of gastric juice, these results indicate that gastric juice ABHD11-AS1 may be a potential biomarker in the screening of gastric cancer.
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Yunben
Y
Yang
Department of Gastroenterology, The Affiliated Hospital, Ningbo University School of Medicine, Ningbo, China.
Yongfu
Y
Shao
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Mengying
M
Zhu
Department of Gastroenterology, The Affiliated Hospital, Ningbo University School of Medicine, Ningbo, China.
Qier
Q
Li
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Fang
F
Yang
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Xuwen
X
Lu
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Chunjing
C
Xu
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Bingxiu
B
Xiao
Zhenhai Middle School, Ningbo, China.
Yanke
Y
Sun
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China. junmingguo@yahoo.com.
Junming
J
Guo
D000368:Aged; D014408:Biomarkers, Tumor / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000097:blood; D055088:Early Detection of Cancer; D005260:Female; D005750:Gastric Juice / Q000737:chemistry*; D005756:Gastritis / Q000175:diagnosis; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding / Q000737:chemistry*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D057057:Serine Proteases / Q000378:metabolism; D013270:Stomach / Q000737:chemistry; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000175:diagnosis
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D062085:RNA, Long Noncoding; C568817:ABHD11 protein, human; D057057:Serine Proteases
ABHD11-AS1; Gastric cancer; Gastric juice; Gene diagnosis; Long noncoding RNA; Tumor marker