MiR-133b-5p regulates the expression of the heat shock protein 70 during rat neuronal cell apoptosis induced by the gp120 V3 loop peptide.
Neuronal cell dysfunction and apoptosis, the main causes of HIV-associated dementia, and its underlying mechanism are important unsolved health problems. Many research reports suggest that miRNAs regulate HIV-1-induced apoptosis. We used the HIV-1 gp120 V3 Loop peptide to induce primary rat cortical neurons apoptosis. Next, we used a microRNA microarray to identify the significant changes of miRNA in the rat cortical neurons treated with the gp120 V3 loop peptide. We used western blot and real-time PCR to measure the regulation of heat shock protein 70 by rno-miR-133b-5p. In response to the gp120 V3 loop peptide treatment, rat cortical neurons exhibited 11 up-regulated and 21 down-regulated miRNAs. We further examined miR-133b-5p, a microRNA that was up-regulated more than 118-fold. In addition, both HSP70 mRNA and protein expression were dose-dependent in rats cortical neurons treated with gp120 V3 loop peptide for 48 hr. MiR-133b-5p could regulate heat shock protein 70 (HSP70) at both transcription and translation levels. Rno-miR-133b-5p might be less significant for the gp120 V3 loop peptide induced neuron apoptosis. Thus, we discovered a potential new target for the regulation of HIV-1 gp120- induced apoptosis.
Department of Pharmacy, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, P.R. China.
Chenglai
C
Xia
Department of Dermatology and Rheumatology, Foshan Maternity & Child Heath Care Hospital, Foshan, 528000, China.
Yantao
Y
Cai
Department of Reproductive Medicine Center, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, P.R. China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou Medical University at Guangzhou, 63 Duobao Road, Guangzhou, GD, 510150, China.
Yuyi
Y
Lin
Department of Reproductive Medicine Center, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, P.R. China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou Medical University at Guangzhou, 63 Duobao Road, Guangzhou, GD, 510150, China.
Ronghua
R
Guan
Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou Medical University at Guangzhou, 63 Duobao Road, Guangzhou, GD, 510150, China.
Guohong
G
Xiao
Department of Reproductive Medicine Center, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, P.R. China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou Medical University at Guangzhou, 63 Duobao Road, Guangzhou, GD, 510150, China.
Jie
J
Yang
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D002478:Cells, Cultured; D015536:Down-Regulation; D005786:Gene Expression Regulation; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D046228:Microarray Analysis; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D018840:HSP70 Heat-Shock Proteins; C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; D010446:Peptide Fragments
apoptosis; gp120; heat shock protein 70; miR-133b-5p; microRNA