Silver Enhancement for Transmission Electron Microscopy Imaging of Antibody Fragment-Gold Nanoparticles Conjugates Immobilized on Ordered Mesoporous Silica.
Ordered mesoporous silica (OMS) materials are receiving great attention as possible carriers for valuable but unstable drugs as, for example, therapeutic proteins. A key issue is to prove that the therapeutic protein is effectively able to penetrate the pores of OMS during the adsorption step. Here, we immobilized an antibody fragment [F(ab')GAMIgG] conjugated with ultrasmall gold nanoparticles (GNPs) onto amino-functionalized SBA-15 (SBA-NH2) mesoporous silica. The aim of this work is the visualization of the location of the conjugates adsorbed onto SBA-NH2 with transmission electron microscopy (TEM). Because of the ultrasmall size of GNPs (<1 nm), we use the silver enhancement procedure to amplify their size. In this procedure, ultrathin sections of conjugate-loaded SBA-NH2 particles are prepared by a ultramicrotome sectioning technique. The ultrasmall GNPs located on the top side of the 70-90 nm thick slices act as microcrystallization nucleation sites for the deposition of reduced metallic silver. Consequently, the ultrasmall GNPs increase their size. This allows for the direct imaging of the conjugates adsorbed. We clearly localize the F(ab')GAMIgG-GNPs conjugates either on the external surface of the particles or inside the mesopores of SBA-NH2 through TEM.
Department of Biomedical Sciences, and ‡Department of Chemical and Geological Sciences, University of Cagliari , CSGI and CNBS, Cittadella Universitaria, Strada Statale 554 Bivio Sestu, 09042 Monserrato Cagliari, Italy.
Marco
M
Piludu
Department of Biomedical Sciences, and ‡Department of Chemical and Geological Sciences, University of Cagliari , CSGI and CNBS, Cittadella Universitaria, Strada Statale 554 Bivio Sestu, 09042 Monserrato Cagliari, Italy.
Luca
L
Medda
Department of Biomedical Sciences, and ‡Department of Chemical and Geological Sciences, University of Cagliari , CSGI and CNBS, Cittadella Universitaria, Strada Statale 554 Bivio Sestu, 09042 Monserrato Cagliari, Italy.
Francesca
F
Cugia
Department of Biomedical Sciences, and ‡Department of Chemical and Geological Sciences, University of Cagliari , CSGI and CNBS, Cittadella Universitaria, Strada Statale 554 Bivio Sestu, 09042 Monserrato Cagliari, Italy.
Maura
M
Monduzzi
Department of Biomedical Sciences, and ‡Department of Chemical and Geological Sciences, University of Cagliari , CSGI and CNBS, Cittadella Universitaria, Strada Statale 554 Bivio Sestu, 09042 Monserrato Cagliari, Italy.
Andrea
A
Salis
D000906:Antibodies / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
D000906:Antibodies; D012834:Silver; D006046:Gold; D012822:Silicon Dioxide