Carbon Nanotube-Patterned Surface-Based Recognition of Carcinoembryonic Antigens in Tumor Cells for Cancer Diagnosis.
It has been of high significance to devise a biochemical analytical tool kit enabling the detection of few circulating tumor cells (CTCs) for early diagnosis of cancer. Despite recent effort made to detect few CTCs, it is still challenging to sense such cells with their low concentration and/or the minute amount of marker proteins expressed on few CTCs. In this work, we report the label-free recognition of carcinoembryonic antigens (CEAs) expressed on few CTCs by using a carbon nanotube (CNT) sensor coupled with scanning probe microscopy imaging for cancer diagnosis. It is shown that a CNT-patterned surface is able to specifically capture the CEA molecules in the whole cell lysate of CTCs with their concentration even up to 10(-3) cells/mL. Our work sheds light on our bioassay based on a CNT-patterned surface for highly sensitive, label-free detection of marker proteins expressed on few tumor cells, which may open a new avenue in early diagnosis of cancer by providing a novel biochemical analysis tool kit.
†Institute for Molecular Sciences, Seoul 120-749, Republic of Korea.|‡Department of Biomedical Engineering, Yonsei University, Wonju 220-710, Republic of Korea.
Taeyun
T
Kwon
¶Department of Mechanical Engineering, Korea University, Seoul 136-701, Republic of Korea.
Jinsung
J
Park
†Institute for Molecular Sciences, Seoul 120-749, Republic of Korea.|‡Department of Biomedical Engineering, Yonsei University, Wonju 220-710, Republic of Korea.
Gyudo
G
Lee
†Institute for Molecular Sciences, Seoul 120-749, Republic of Korea.|‡Department of Biomedical Engineering, Yonsei University, Wonju 220-710, Republic of Korea.
Kihwan
K
Nam
§Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Republic of Korea.
Yong-Min
YM
Huh
⊥Department of Molecular Biology, Dankook University, Yongin 448-701, Republic of Korea.
Seong-Wook
SW
Lee
§Department of Radiology, College of Medicine, Yonsei University, Seoul 120-752, Republic of Korea.
Jaemoon
J
Yang
∥School of Nano-Bioscience and Chemical Engineering and Interdisciplinary School of Green Energy, Ulsan National Institute of Science and Technology, Ulsan 689-798, Republic of Korea.
Chang Young
CY
Lee
†Institute for Molecular Sciences, Seoul 120-749, Republic of Korea.|#Biomechanics Laboratory, College of Sport Science, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Kilho
K
Eom
aptamer; cancer diagnosis; carbon nanotube; carcinoembryonic antigen; circulating tumor cell