Custom-made Microdialysis Probe Design.
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand to monitor physiological, pharmacological and pathological changes in cerebrospinal fluid. Unfortunately, commercial probes are expensive for research groups in public institutions. In this work, a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10. The microdialysis probe consists of a polysulfone membrane with a molecular cut-off of 30 kDa. Probe in vitro recoveries of substances with different molecular weight (in the range of 100-1,600 Da) and different physicochemical properties are compared. The probe yields an in vitro recovery of approximately 20% for the small compounds glucose, lactate, acetylcholine and ATP. In vitro recoveries for neuropeptides with a molecular weight between 1,000-1,600 Da amount to 2-6%. Thus, while the higher molecular weight of the neuropeptides lowered in vitro recovery values, dialysis of compounds in the lower range (up to 500 Da) of molecular weights has no great impact on the in vitro recovery rate. The present method allows utilization of a dialysis membrane with other cut-off value and membrane material. Therefore, this custom-made probe assembly has the advantage of sufficient flexibility to dialyze substances in a broad molecular weight range. Here, we introduce a microdialysis probe with an exchange length of 2 mm, which is applicable for microdialysis in mouse and rat brain regions. However, dimensions of the probe can easily be adapted for larger exchange lengths to be used in larger animals.
Department of Pharmacology, Goethe University of Frankfurt.
Jana
J
Lietsche
Department of Pharmaceutical Chemistry, Goethe University of Frankfurt.
Jan
J
Gorka
Department of Clinical Pharmacology, Goethe University of Frankfurt.
Stefanie
S
Hardt
Department of Pharmaceutical Chemistry, Goethe University of Frankfurt.
Michael
M
Karas
Department of Pharmacology, Goethe University of Frankfurt; klein@em.uni-frankfurt.de.
Jochen
J
Klein
D000818:Animals; D001923:Brain Chemistry; D004867:Equipment Design / Q000379:methods; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D051379:Mice; D017551:Microdialysis / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D008970:Molecular Weight; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods; D009479:Neuropeptides / Q000032:analysis; D011108:Polymers / Q000737:chemistry; D051381:Rats; D013450:Sulfones / Q000737:chemistry
D009479:Neuropeptides; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D019344:Lactic Acid; D005947:Glucose