Comparison of oxytetracycline degradation behavior in pig manure with different antibiotic addition methods.
Using manure collected from swine fed with diet containing antibiotics and antibiotic-free swine manure spiked with antibiotics are the two common methods of studying the degradation behavior of veterinary antibiotic in manure in the environment. However, few studies had been conducted to co-compare these two different antibiotic addition methods. This study used oxytetracycline (OTC) as a model antibiotic to study antibiotic degradation behavior in manure under the above two OTC addition methods. In addition, the role of microorganisms present in the manure on degradation behavior was also examined. The results showed that degradation half-life of OTC in manure from swine fed OTC (9.04 days) was significantly shorter than that of the manure directly treated with OTC (9.65 days). Concentration of 4-epi-OTC in manure from swine fed OTC peaked earlier than that in manure spiked with OTC, and the degradation rates of 4-epi-OTC and α-apo-OTC in the manure from swine fed OTC were faster, but the peak concentrations were lower, than those in manure spiked with OTC. Bacterial diversity and relative abundance of Bacillus cereus data demonstrated that sterilization of the manure before experiment significantly decreased OTC degradation rate in both of the addition methods. Results of the present study demonstrated that the presence of the metabolites (especially 4-epi-OTC) and microorganisms had significant influence on OTC degradation.
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.|Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou, 510642, China.
Yan
Y
Wang
College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Guixiu
G
Chen
Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, 43400, Malaysia.
Juanboo
J
Liang
Nanhai Entry-Exit Inspection and Quarantine Bureau, Foshan, 528200, China.
Yongde
Y
Zou
College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Xin
X
Wen
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.|Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou, 510642, China.
Xindi
X
Liao
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China. wuyinbao@scau.edu.cn.|Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou, 510642, China. wuyinbao@scau.edu.cn.
Yinbao
Y
Wu
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D002681:China; D004785:Environmental Pollutants / Q000032:analysis*; D006207:Half-Life; D008372:Manure / Q000032:analysis* / Q000382:microbiology; D010118:Oxytetracycline / Q000032:analysis*; D064210:Secondary Metabolism; D013552:Swine
D000900:Anti-Bacterial Agents; D004785:Environmental Pollutants; D008372:Manure; D010118:Oxytetracycline
Bacterial diversity; Degradation behavior; Oxytetracycline; Relative abundance of Bacillus cereus; Swine manure · 4-epi-OTC · α-apo-OTC