Time and Concentration-Dependent Therapeutic Potential of Silver Nanoparticles in Cervical Carcinoma Cells.
Silver nanoparticles (AgNPs) have well-known anti-bacterial properties and have been widely used in daily life as various medical and general products. There is limited information available on the cytotoxicity of AgNPs. Therefore, the present study aimed to investigate the cytotoxicity of AgNPs in HeLa cells. Cytotoxicity and apoptosis have been observed in the AgNPs treated in the HeLa cells. Sulphorhodamine-B assay (SRB assay) showed the cytotoxic effect in the AgNP-treated HeLa cells. Inverted microscope, fluorescence microscope, and confocal laser scanning microscope (CLSM) analyses showed the apoptosis-induced morphological changes such as rounding in shape, nuclear fragmentation, cytoplasm reduction, loss of adhesion, and reduced cell volume. Necrosis and apoptosis were observed in the AgNP-treated HeLa cells by DNA fragmentation study. Mitochondria-derived reactive oxygen species (ROS) have increased in AgNP-treated HeLa cells. Up-regulation of messenger RNA (mRNA) expression of p53, bax, and caspase 3 were found in AgNP-treated HeLa cells. Caspase 3 enzyme activity was found to increase in AgNP-treated HeLa cells. The AgNPs showed the right cytotoxic effect in cervical carcinoma cells. Our results suggest that metal-based nanoparticles might be a potential candidate for the treatment of cervical cancer.
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
Muthuraman
M
Pandurangan
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
Gansukh
G
Enkhtaivan
Department of Molecular Biotechnology, Konkuk University, Seoul, South Korea.
Baskar
B
Venkitasamy
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
Bhupendra
B
Mistry
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
Rafi
R
Noorzai
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea. byj@sunbio2.com.
Bong Yeon
BY
Jin
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea. frenzram1980@gmail.com.
Doo Hwan
DH
Kim
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D003603:Cytotoxins / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D000970:Antineoplastic Agents; D003603:Cytotoxins; D017382:Reactive Oxygen Species; D012834:Silver
AgNPs; Apoptosis; Confocal microscope; Fluorescence microscope; HeLa cells; SRB