[METHODS FOR DETECTION OF ANTIBODIES TO HLA ANTIGENS IN PATIENTS AWAITING KIDNEY TRANSPLANTATION AND COMBINED PANCREAS-KIDNEY TRANSPLANTATION].
The best possibility to treat chronic renal disease is renal transplantation. Especially important fact in transplantation is the percentage of so-called panel reactive antibody (PRA) that is focused on the human leukocyte antigen. There are several methods to determine the percentage of PRA in sensitized patients awaiting kidney transplants. The most important is the complement-dependent cytotoxicity. A higher value of PRA implies greater likelihood of positive cross-match with random donor and lower probability of receiving a transplant. Comparing the sensitivity of laboratory tests for determination of PRA percentage in patient serum, it is concluded that ELISA and flow cytometry proved to be more sensitive and specific.
Mirela
M
Ančić
Blaženko
B
Maravić
Maja
M
Radman
Vedran
V
Kovačić
D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation / Q000379:methods*; D016035:Pancreas Transplantation / Q000379:methods; D014019:Tissue Donors
D000906:Antibodies; D006680:HLA Antigens