Facile Method To Prepare Microcapsules Inspired by Polyphenol Chemistry for Efficient Enzyme Immobilization.
In this study, a method inspired by polyphenol chemistry is developed for the facile preparation of microcapsules under mild conditions. Specifically, the preparation process includes four steps: formation of the sacrificial template, generation of the polyphenol coating on the template surface, cross-linking of the polyphenol coating by cationic polymers, and removal of the template. Tannic acid (TA) is chosen as a representative polyphenol coating precursor for the preparation of microcapsules. The strong interfacial affinity of TA contributes to the formation of polyphenol coating through oxidative oligomerization, while the high reactivity of TA is in charge of reacting/cross-linking with cationic polymer polyethylenimine (PEI) through Schiff base/Michael addition reaction. The chemical/topological structures of the resultant microcapsules are simultaneously characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier Transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), etc. The wall thickness of the microcapsules could be tailored from 257±20 nm to 486±46 nm through changing the TA concentration. The microcapsules are then utilized for encapsulating glucose oxidase (GOD), and the immobilized enzyme exhibits desired catalytic activity and enhanced pH and thermal stabilities. Owing to the structural diversity and functional versatility of polyphenols, this study may offer a facile and generic method to prepare microcapsules and other kinds of functional porous materials.
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, China.
Shaohua
S
Zhang
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, China.
Zhongyi
Z
Jiang
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, China.
Xiaoli
X
Wang
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, China.
Chen
C
Yang
School of Environmental Science and Engineering, Tianjin University , Tianjin 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, China.
Jiafu
J
Shi
D055162:Biocatalysis; D002214:Capsules / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D056951:Photoelectron Spectroscopy; D011094:Polyethyleneimine / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013634:Tannins / Q000737:chemistry; D013696:Temperature
D002214:Capsules; D004800:Enzymes, Immobilized; D059808:Polyphenols; D012545:Schiff Bases; D013634:Tannins; D011094:Polyethyleneimine; D005949:Glucose Oxidase
enzyme immobilization; glucose oxidase; microcapsules; polyphenol chemistry; tannic acid