Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor.
We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu(2+)) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu(2+)-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu(2+) deficiency in practical applications.
Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Shofarul
S
Wustoni
Sho
S
Hideshima
Shigeki
S
Kuroiwa
Takuya
T
Nakanishi
Yasuro
Y
Mori
Tetsuya
T
Osaka
D015374:Biosensing Techniques / Q000295:instrumentation*; D003300:Copper / Q000097:blood*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D057230:Limit of Detection; D008297:Male; D008958:Models, Molecular; D011328:Prions / Q000737:chemistry*; D011487:Protein Conformation; D014173:Transistors, Electronic
D055767:Immobilized Proteins; D011328:Prions; D003300:Copper