Analysis of electron donors in photosystems in oxygenic photosynthesis by photo-CIDNP MAS NMR.
Both photosystem I and photosystem II are considerably similar in molecular architecture but they operate at very different electrochemical potentials. The origin of the different redox properties of these RCs is not yet clear. In recent years, insight was gained into the electronic structure of photosynthetic cofactors through the application of photochemically induced dynamic nuclear polarization (photo-CIDNP) with magic-angle spinning NMR (MAS NMR). Non-Boltzmann populated nuclear spin states of the radical pair lead to strongly enhanced signal intensities that allow one to observe the solid-state photo-CIDNP effect from both photosystem I and II from isolated reaction center of spinach (Spinacia oleracea) and duckweed (Spirodela oligorrhiza) and from the intact cells of the cyanobacterium Synechocystis by (13)C and (15)N MAS NMR. This review provides an overview on the photo-CIDNP MAS NMR studies performed on PSI and PSII that provide important ingredients toward reconstruction of the electronic structures of the donors in PSI and PSII.
University of Leipzig, Institute of Analytical Chemistry, Johannisallee 29, D-04103 Leipzig, Germany.
M
M
Najdanova
University of Leiden, Leiden Institute of Chemistry, Einsteinweg 55, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
G J
GJ
Janssen
University of Leiden, Leiden Institute of Chemistry, Einsteinweg 55, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
H J M
HJ
de Groot
University of Leipzig, Institute of Analytical Chemistry, Johannisallee 29, D-04103 Leipzig, Germany.
J
J
Matysik
University of Leiden, Leiden Institute of Chemistry, Einsteinweg 55, P.O. Box 9502, 2300 RA Leiden, The Netherlands; University of Leipzig, Institute of Medical Physics and Biophysics, Härtelstr. 16-18, D-04107 Leipzig, Germany. Electronic address: alia.aliamatysik@medizin.uni-leipzig.de.
A
A
Alia
D004579:Electron Transport; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism
D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010100:Oxygen
Electron donor; Photo-CIDNP; Photosynthesis; Photosystem I; Photosystem II; Redox potential; Solid-state NMR