In Vitro Angiogenesis Assays.
Angiogenesis is a key process mediated by VEGF, and the study of angiogenic mechanisms has clinical importance in a variety of pathological conditions, including ischemic heart disease and cancer. In vitro angiogenesis assays are based on the innate ability of endothelial cells to migrate and form tube like structures in response to VEGF stimulation. Although they are arguably not as physiologically relevant as in vivo angiogenesis models they still represent a relatively quick and useful method for looking at VEGF function. There are several different methods for studying in vitro angiogenesis and these are detailed here along with protocols for image capture and analysis.
Centre for Cardiovascular Biology and Medicine, Division of Medicine, University College London, Rayne Building, 5 University Street, London, WC1E 6JF, UK, i.evans@ucl.ac.uk.
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Evans
D002478:Cells, Cultured; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D042783:Endothelial Cells / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin; D018919:Neovascularization, Physiologic; D011509:Proteoglycans
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen