Additive solutions differentially affect metabolic and functional parameters of platelet concentrates.
BACKGROUND
Pathogen inactivation (PI) of platelet concentrates with extension of shelf life to 7 days requires the use of platelet additive solutions (PAS). We examined the quality of platelets resuspended in three different PAS stored for up to 7 days.


METHODS
Twelve triple adult dose platelet concentrates (PC) were collected using the TrimaAccel® collection system. Each highly concentrated product was divided into three equal parts, and the additive solutions (Composol® or SSP+® or Intersol™) were added to a final concentration of 56% PAS and 44% plasma. Samples were drawn on days 1, 5 and 7 to measure pH, glucose, lactate dehydrogenase (LDH), lactate, mean platelet volume (MPV) and the aggregation response to collagen and the thrombin receptor agonist peptide-6. Further, p-selectin expression on platelets was assessed.


RESULTS
No statistically significant changes were observed for pH and MPV during 7 days of storage in all PAS containing PCs, whereas glucose decreased and LDH and lactate increased over time (P < 0·05). These changes were particularly evident in Intersol PCs on days 5 and 7 compared with Composol® PCs or SSP+® PCs (P < 0·05). Platelets from Intersol PCs exhibited the highest baseline activation of p-selectin and showed reduced collagen- and TRAP-6-induced aggregation.


CONCLUSIONS
Resuspension of platelets in Intersol for 7 days results in increased platelet activation and platelet metabolism compared with SSP+® or Composol®. Further clinical studies are needed to evaluate whether the observed differences in PAS-PCs affect the recovery rate or the life span of transfused platelets.
Department of Blood Group Serology and Transfusion Medicine, Medical University, Vienna, Austria.
G C
GC
Leitner
Department of Hematology, Hanuschkrankenhaus, Vienna, Austria.
J
J
List
Department of Blood Group Serology and Transfusion Medicine, Medical University, Vienna, Austria.
M
M
Horvath
Department of Blood Group Serology and Transfusion Medicine, Medical University, Vienna, Austria.
B
B
Eichelberger
Department of Blood Group Serology and Transfusion Medicine, Medical University, Vienna, Austria.
S
S
Panzer
Department of Laboratory Medicine, Medical University, Vienna, Austria.
P
P
Jilma-Stohlawetz
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D019007:P-Selectin / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D015539:Platelet Activation; D010979:Platelet Function Tests; D011310:Preservatives, Pharmaceutical / Q000009:adverse effects* / Q000494:pharmacology
D019007:P-Selectin; D010446:Peptide Fragments; D011310:Preservatives, Pharmaceutical; C546937:SELP protein, human; C082835:thrombin receptor peptide (42-47)
additive solution; aggregation capacity; platelets; storage lesion