Cellular effects of magnetic nanoparticles explored by atomic force microscopy.
The investigation of subtle change of cells exposed to nanomaterials is extremely essential but also challenging for nanomaterial-based biological applications. In this study, atomic force microscopy (AFM) was employed to investigate the effects of iron-iron oxide core-shell magnetic nanoparticles on the mechanical properties of bovine articular chondrocytes (BACs). After being exposed to the nanoparticles even at a high nanoparticle-concentration (50 μg mL(-1)), no obvious difference was observed by using conventional methods, including the WST-1 assay and live/dead staining. However a significant difference of Young's modulus of the cells was detected by AFM even when the concentration of nanoparticles applied in the cell culture medium was low (10 μg mL(-1)). The difference of cellular Young's modulus increased with the increase of nanoparticle concentration. AFM was demonstrated to be a useful tool to identify the subtle change of cells when they were exposed to nanomaterials even at a low concentration.
Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Hongli
H
Mao
Jingchao
J
Li
Ida
I
Dulińska-Molak
Naoki
N
Kawazoe
Yoshihiko
Y
Takeda
Hiroaki
H
Mamiya
Guoping
G
Chen
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*
D058185:Magnetite Nanoparticles