Gene expression profiling predicts pathways and genes associated with Parkinson's disease.
This study was aimed to explore the molecular mechanism of Parkinson's disease (PD) development and discover underlying pathways and genes associated with PD. The microarray data of GSE22491 containing 10 samples from PD patients and 8 samples from healthy controls (HC) were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified by paired t-test. Then the DEGs were performed cluster and principal component analyses followed by Gene Ontology (GO) and pathway enrichment analyses and protein-protein interaction (PPI) network construction. Total 176 up-regulated DEGs and 49 down-regulated DEGs were identified. Totally, 39 GO terms and 72 pathways were closely related to PD. Pathway of neuronal system was enriched by 10 DEGs such as synapsin I (SYN1), glutamate receptor, ionotropic, N-methyl-D-aspartate 1 (GRIN1) and GRIN2D. In the PPI networks, 18 hub genes were obtained, such as GRIN2D and discs, large (Drosophila) homolog-associated protein 3 (DLGAP3). The pathway of neuronal system and its enriched DEGs may play important roles in PD progression. The DEGs such as SYN1, GRIN1, GRIN2D and DLGAP3 may become promising candidate genes for PD.
Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China.
Shuang
S
Liu
Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China.
Yong
Y
Zhang
Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China.
Hong
H
Bian
Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China. xiaohonglixhlx@163.com.
Xiaohong
X
Li
D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D046228:Microarray Analysis; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism*; D025341:Principal Component Analysis; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
Differentially expressed genes; Hub protein; Parkinson’s disease; Pathway enrichment analysis; Protein–protein interaction network