[Identification of Hydrocarbon-Oxidizing Dietzia Bacteria from Petroleum Reservoirs Based on Phenotypic Properties and Analysis of the 16S rRNA and gyrB Genes].
The taxonomic position of hydrocarbon-oxidizing bacterial strains 263 and 32d isolated from formation water of the Daqing petroleum reservoir (PRC) was determined by polyphasic taxonomy techniques, including analysis of the 16S rRNA and the gyrB genes. The major chemotaxonomic characteristics of both strains, including the IV type cell wall, composition of cell wall fatty acids, mycolic acids, and menaquinones, agreed with those typical of Dietzia strains. The DNA G+C content of strains 263 and 32d were 67.8 and 67.6 mol%, respectively. Phylogenetic analysis of the 16S rRNA gene of strain 32d revealed 99.7% similarity to the gene of D. maris, making it possible to identify strain 32d as belonging to this species. The 16S rRNA gene sequence of strain 263 exhibited 99.7 and 99.9% similarity to those of D. natronolimnaea and D. cercidiphylli YIM65002(T), respectively. Analysis of the gyrB genes of the subterranean isolates and of a number of Dietzia type strains confirmed classiffication of strain 32d as a D. maris strain and of strain 263, as a D. natronolimnaea strain. A conclusion was made concerning higher resolving power of phylogenetic analysis of the gyrB gene compared to the 16S rRNA gene analysis in the case of determination of the species position of Dietzia isolates.
T N
TN
Nazina
E S
ES
Shumkova
D Sh
DSh
Sokolova
T L
TL
Babich
M V
MV
Zhurina
Yan-Fen
YF
Xue
G A
GA
Osipov
A B
AB
Poltaraus
T P
TP
Tourova
D000192:Actinomycetales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D006838:Hydrocarbons / Q000378:metabolism*; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D010084:Oxidation-Reduction; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D005227:Fatty Acids; D006838:Hydrocarbons; D009171:Mycolic Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase