Redox cycling-amplified enzymatic Ag deposition and its application in the highly sensitive detection of creatine kinase-MB.
This communication reports a novel enzymatic Ag-deposition scheme combined with chemical-chemical redox cycling by reduced β-nicotinamide adenine dinucleotide. This novel scheme allows a higher Ag-deposition rate than a scheme using only enzymatic Ag deposition. Therefore, it can be applied for the highly sensitive detection of a cardiac biomarker, creatine kinase-MB.
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 609-735, Korea. hyang@pusan.ac.kr.
Al-Monsur
AM
Jiaul Haque
Jihye
J
Kim
Gorachand
G
Dutta
Sinyoung
S
Kim
Haesik
H
Yang
D015415:Biomarkers / Q000032:analysis; D052279:Creatine Kinase, MB Form / Q000032:analysis*; D004798:Enzymes / Q000737:chemistry*; D057230:Limit of Detection; D010084:Oxidation-Reduction; D012834:Silver / Q000737:chemistry*
D015415:Biomarkers; D004798:Enzymes; D012834:Silver; D052279:Creatine Kinase, MB Form