Callicarpa longissima extract, carnosol-rich, potently inhibits melanogenesis in B16F10 melanoma cells.
Cosmetic industries focus on developing materials and resources that regulate skin pigmentation. Melanin, the major pigment in human skin, protects the skin against damage from ultraviolet light. An ethanolic extract of the leaves of Callicarpa longissima inhibits melanin production in B16F10 mouse melanoma cells by suppressing microphthalmia-associated transcription factor (MITF) gene expression. Following purification and analysis using liquid chromatography-mass spectrometry (LC-MS), NMR, and biochemical assays, carnosol was determined to be responsible for the major inhibitory effect of the C. longissima extract on melanin production. Carnosol is an oxidative product of carnosic acid, whose presence in the extract was also confirmed by an authentic reference. The carnosol and carnosic acid content in the extract was approximately 16% (w/w). These results suggest that C. longissima is a novel, useful, and attractive source of skin-whitening agents.
Institute for Molecular Science of Medicine, Aichi Medical University, 1-1, Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.|Department of Research and Development, Momotani-juntenkan Co. Ltd, 2-4-30, Ichioka, Minato-ku, Osaka, 552-0012, Japan.
Minori
M
Yamahara
Tanegashima Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 17007-2, Matsubarayama, Noma, Nakatane-cho, Kumage-gun, Kagoshima, 891-3604, Japan.
Koji
K
Sugimura
Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan.
Ayako
A
Kumagai
Tsukuba Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 1-2, Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
Hiroyuki
H
Fuchino
Department of Research and Development, Momotani-juntenkan Co. Ltd, 2-4-30, Ichioka, Minato-ku, Osaka, 552-0012, Japan.|Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan.
Azusa
A
Kuroi
Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan.
Mai
M
Kagawa
Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan.
Yumi
Y
Itoh
Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, 564-8680, Japan.
Hidehisa
H
Kawahara
Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, 564-8680, Japan.
Yasuo
Y
Nagaoka
Tanegashima Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 17007-2, Matsubarayama, Noma, Nakatane-cho, Kumage-gun, Kagoshima, 891-3604, Japan.|Tsukuba Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 1-2, Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
Osamu
O
Iida
Tanegashima Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 17007-2, Matsubarayama, Noma, Nakatane-cho, Kumage-gun, Kagoshima, 891-3604, Japan.|Tsukuba Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 1-2, Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
Nobuo
N
Kawahara
Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan. mukuharu22@yahoo.co.jp.
Hiroshi
H
Takemori
Institute for Molecular Science of Medicine, Aichi Medical University, 1-1, Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
Hideto
H
Watanabe
D045784:Abietanes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D032408:Callicarpa / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D062327:Skin Lightening Preparations / Q000494:pharmacology; D012880:Skin Pigmentation / Q000187:drug effects
D045784:Abietanes; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D010936:Plant Extracts; D062327:Skin Lightening Preparations; C068623:carnosol; C018381:salvin
B16F10; Carnosic acid; Carnosol; MITF; Melanogenesis