Comparative transcriptome analysis reveals that the extracellular matrix receptor interaction contributes to the venous metastases of hepatocellular carcinoma.
Hepatocellular carcinoma (HCC) is the most common type of liver cancer in the world. Portal vein tumor thrombus (PVTT) is one of the most serious complications of HCC and is strongly correlated with a poor prognosis for HCC patients. However, the detailed mechanism of PVTT development remains to be explored. In this study, we present a large-scale transcriptome analysis, by RNA sequencing, of 11 patients diagnosed with HCC with PVTT. The dysregulated genes between HCC and PVTT suggested that the extracellular matrix receptor interaction was correlated with the venous metastases of HCC. Among all of the recurrent alternative splicing events, we identified exon 6 skipping of RPS24, which is likely to be a cancer driver. We also identified five common fusion genes between HCC and its corresponding PVTT samples, including ARID1A-GPATCH3, MDM1-NUP107, PTGES3-RARG, PRLR-TERT, and C9orf3-TMC1. All of these findings broaden our knowledge of PVTT development and may also contribute to the diagnosis and treatment of HCC patients with PVTT.
Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Hong
H
Zhang
Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Junyi
J
Ye
Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Xiaoling
X
Weng
Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.
Fatao
F
Liu
Institutes of Biomedical Sciences, Fudan University, Shanghai, China; Bio-X Center, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Lin
L
He
Bio-X Center, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. Electronic address: zhoudaizhan@gmail.com.
Daizhan
D
Zhou
Institutes of Biomedical Sciences, Fudan University, Shanghai, China. Electronic address: superliuyun@gmail.com.
Yun
Y
Liu
D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000235:genetics* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism; D011169:Portal Vein / Q000473:pathology*; D011379:Prognosis; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D059467:Transcriptome; D020246:Venous Thrombosis / Q000209:etiology / Q000235:genetics* / Q000473:pathology
D015514:Oncogene Proteins, Fusion; C489053:RPS24 protein, human; D012269:Ribosomal Proteins
Hepatocellular carcinoma; alternative splicing; differentially expressed genes; gene fusion; portal vein tumor thrombus