B-cell lymphoma-2 localization in the female reproductive tract of the Chinese soft-shelled turtle, Pelodiscus sinensis and its relationship with sperm storage.
The aim of the present study was to investigate the expression and localization of B-cell lymphoma-2 (Bcl-2) in the oviduct of the Chinese soft-shelled turtle, Pelodiscus sinensis, during the reproductive cycle to analyze the relationship between Bcl-2 and sperm storage. Bcl-2 expression was confirmed in the P. sinensis oviduct by western blot analysis. Hematoxylin-eosin staining showed that female P. sinensis stored sperm from November to April of the following year. The oviduct showed positive immunostaining for Bcl-2 of epithelial ciliated cells, gland ducts, and gland cells. Bcl-2 expression in the oviduct was associated with sperm storage occurrence. This indicates that the survival factor Bcl-2 may play a role in P. sinensis sperm storage.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Yuan
Y
Le
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Shaofan
S
Chen
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Lisi
L
Hu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Linli
L
Zhang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Shakeeb
S
Ullah
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Tengfei
T
Liu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Ping
P
Yang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Yi
Y
Liu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
Qiusheng
Q
Chen
D000818:Animals; D005260:Female; D008297:Male; D010057:Oviducts / Q000033:anatomy & histology* / Q000737:chemistry* / Q000166:cytology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D012098:Reproduction; D013094:Spermatozoa / Q000737:chemistry* / Q000166:cytology; D014426:Turtles; D014599:Uterus / Q000033:anatomy & histology / Q000737:chemistry / Q000166:cytology
D019253:Proto-Oncogene Proteins c-bcl-2
Bcl-2; Chinese soft-shelled turtle; Western blot; immunohistochemistry; oviduct