MUTYH Gene Polymorphisms as Risk Factors for Rheumatoid Arthritis.
OBJECTIVE
MUTYH glycosylase involved in DNA repair pathways may be associated with the risk of autoimmune diseases such as rheumatoid arthritis (RA). Therefore, the association between polymorphisms in the MUTYH gene and RA was evaluated.


METHODS
We recruited 192 RA patients and 192 healthy subjects in Taiwan. The 4 MUTYH polymorphisms (rs3219463, rs3219476, rs3219489, and rs3219493) were detected and haplotype analysis was performed using the Bayesian method. The genotype and allelic frequency distributions of the polymorphisms in both RA patients and healthy patients were compared by the chi-square test.


RESULTS
Comparison of the genotype/allele frequencies between individuals with RA and the control groups revealed significant differences in 2 MUTYH gene polymorphisms, rs3219463 and rs3219476. After we performed a haplotype-specific analysis, the haplotypes Ht6-GTGC and Ht8-GGCG had lower presenting rates in RA patients than in the control groups. Furthermore, the genotype frequency of rs3219463 G/  was significantly increased among patients with immunoglobulin M rheumatoid factors, whereas that of rs3219476 was not.


CONCLUSIONS
We demonstrated that the rs3219463 and rs3219476 polymorphisms in RA patients from a Taiwan Chinese population were associated with disease susceptibility. These data indicate that the MUTYH gene may play a role in the progression of RA.
Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Yung-Jen
YJ
Kung
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30010, Taiwan.
Kun Shi
KS
Tsai
Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan ; Division of Immunology and Rheumatology, Department of Internal Medicine, China Medical University Hospital, Taichung 40402, Taiwan.
Chung-Ming
CM
Huang
Department of Ophthalmology, China Medical University Hospital, Taichung 40402, Taiwan.
Hui-Ju
HJ
Lin
Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 40227, Taiwan.
Ter-Hsin
TH
Chen
Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yu-An
YA
Hsu
Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan ; Department of Biotechnology, Asia University, Taichung 41354, Taiwan.
Ching-Yao
CY
Chang
Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Yong-San
YS
Huang
Department of Biotechnology, Asia University, Taichung 41354, Taiwan ; School of Chinese Medicine, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan ; Department of Gynecology, China Medical University Hospital, Taichung 40402, Taiwan.
Lei
L
Wan
D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics*; D001483:Base Sequence; D045647:DNA Glycosylases / Q000235:genetics*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D013624:Taiwan / Q000453:epidemiology
D005819:Genetic Markers; D045647:DNA Glycosylases; C061738:mutY adenine glycosylase