Transdermal treatment of the surgical and burned wound skin via phytochemical-capped gold nanoparticles.
The biological activities and therapeutic potential of phytochemical-decorated Au nanoparticles (Phyto-AuNPs) were investigated through the treatment of Phyto-AuNPs on the dorsal skin of rats via transdermal drug delivery process in order to regenerate surgical wounded and burned skin. Two different Phyto-AuNPs were applied to the dorsal skin: gallic acid-isoflavone--covered AuNPs (GI-AuNPs) and protocatechuic acid-isoflavone--covered AuNPs (PI-AuNPs). From the biological activity monitoring, it has been resulted that 5-fold thicker epidermis (ER), 50% reduction of metalloproteinase-1 (MMP-1) level, 3-fold higher superoxide dismutase (SOD) activity were obtained in the Phyto-AuNP-treated group, compared with a vehicle group (deionized water (DI-water) treatment). Moreover, the Phyto-AuNPs treatment on the surgical and burn damaged Sprague-Dawley (SD) rats induced higher expression of vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2). It would be plausible that antioxidant property of Phyto-AuNPs assist the acceleration and activation of biomolecules in the healing mechanism, where Phyto-AuNPs can be potential candidates for skin regeneration and wound healing.
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea; Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary T2N 1N4, Canada.
Jaewook
J
Lee
Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Republic of Korea.
JiEun
J
Kim
Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Republic of Korea.
Jun
J
Go
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea.
Jong Ho
JH
Lee
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea.
Dong-Wook
DW
Han
Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Republic of Korea. Electronic address: dyhwang@pusan.ac.kr.
DaeYoun
D
Hwang
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: jaebeom@pusan.ac.kr.
Jaebeom
J
Lee
D000279:Administration, Cutaneous; D042702:Angiopoietin-2 / Q000096:biosynthesis; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002056:Burns / Q000473:pathology / Q000628:therapy*; D004817:Epidermis / Q000473:pathology; D005707:Gallic Acid / Q000627:therapeutic use; D006046:Gold / Q000008:administration & dosage / Q000627:therapeutic use*; D062385:Hydroxybenzoates / Q000008:administration & dosage / Q000627:therapeutic use; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use; D053768:Metal Nanoparticles; D051379:Mice; D008812:Mice, Hairless; D011183:Postoperative Complications / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014945:Wound Healing
D042702:Angiopoietin-2; D000975:Antioxidants; D062385:Hydroxybenzoates; D061965:Matrix Metalloproteinase Inhibitors; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; C009091:protocatechuic acid; D005707:Gallic Acid; D006046:Gold; D013482:Superoxide Dismutase; D020781:Matrix Metalloproteinase 1
Au nanoparticle; Burned skin-healing; Metalloproteinase inhibition; Phytochemicals; Skin regeneration; Surgically damaged skin repair