Bioactive electrospun fibers of poly(glycerol sebacate) and poly(ε-caprolactone) for cardiac patch application.
Scaffolds for cardiac patch application must meet stringent requirements such as biocompatibility, biodegradability, and facilitate vascularization in the engineered tissue. Here, a bioactive, biocompatible, and biodegradable electrospun scaffold of poly(glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) is proposed as a potential scaffold for cardiac patch application. The fibers are smooth bead free with average diameter = 0.8 ± 0.3 μm, mean pore size = 2.2 ± 1.2 μm, porosity = 62 ± 4%, and permeability higher than that of control biological tissue. For the first time, bioactive PGS-PCL fibers functionalized with vascular endothelial growth factor (VEGF) are developed, the approach used being chemical modification of the PGS-PCL fibers followed by subsequent binding of VEGF via amide bonding. The approach results in uniform immobilization of VEGF on the fibers; the concentrations are 1.0 μg cm(-2) for the PGS-PCL (H) and 0.60 μg cm(-2) for the PGS-PCL (L) samples. The bioactive scaffold supports the attachment and growth of seeded myogenic and vasculogenic cell lines. In fact, rat aortic endothelial cells also display angiogenic features indicating potential for the formation of vascular tree in the scaffold. These results therefore demonstrate the prospects of VEGF-functionalized PGS-PCL fibrous scaffold as promising matrix for cardiac patch application.
Institute of Biomaterials Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
Ranjana
R
Rai
Institute of Biomaterials Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
Marwa
M
Tallawi
Department of Medicine and Pathology, University of Parma, 12-I 43126, Parma, Italy.
Caterina
C
Frati
Department of Medicine and Pathology, University of Parma, 12-I 43126, Parma, Italy.
Angela
A
Falco
Department of Medicine and Pathology, University of Parma, 12-I 43126, Parma, Italy.
Andrea
A
Gervasi
Department of Medicine and Pathology, University of Parma, 12-I 43126, Parma, Italy.
Federico
F
Quaini
Institute of Polymeric Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
Judith A
JA
Roether
Institute of Polymeric Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
Tobias
T
Hochburger
Institute of Polymeric Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
Dirk W
DW
Schubert
Ibt - Immunological and Biochemical Testsystems GmbH Wiesenstr. 17, 88521, Binzwangen, Germany.
Lothar
L
Seik
Department of Civil and Industrial Engineering, Largo Lucio Lazzarino, 56126, Pisa, Italy.
Niccoletta
N
Barbani
Department of Civil and Industrial Engineering, Largo Lucio Lazzarino, 56126, Pisa, Italy.
Luigi
L
Lazzeri
Department of Civil and Industrial Engineering, Largo Lucio Lazzarino, 56126, Pisa, Italy.
Elisabetta
E
Rosellini
Institute of Biomaterials Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
Aldo R
AR
Boccaccini
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003651:Decanoates / Q000737:chemistry; D055119:Elastic Modulus; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005990:Glycerol / Q000031:analogs & derivatives / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology; D010539:Permeability; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry / Q000494:pharmacology
D001672:Biocompatible Materials; D003651:Decanoates; D055767:Immobilized Proteins; D011091:Polyesters; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; C469892:poly(glycerol-sebacate); C016240:polycaprolactone; D005990:Glycerol
cardiac patch; electrospinning; poly(glycerol sebacate); poly(ε-caprolactone); vascular endothelial growth factor