Decitabine enhances stem cell antigen-1 expression in cigarette smoke extract-induced emphysema in animal model.
Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). Despite decades of study, its biological functions remain little known. Sca-1 is a typical marker of bone marrow-derived HSCs, it is also expressed by a mixture of tissue-resident stem, progenitor cells in nonhematopoietic organs. Endothelial progenitor cell (EPC) is a subtype of HSC and contributes to endothelial repair by homing in on locations of injury. Abnormal genetic methylation has been detected in smoking-related diseases. The present study aimed to investigate the lung function and histomorphology, the expression of Sca-1 gene in lung tissues, and bone marrow-derived EPCs in cigarette smoke extract (CSE)-induced emphysema mice, and to further determine whether Decitabine (Dec), the most widely used inhibitor of DNA methylation, could protect against the damages caused by CSE. The results of the present study demonstrated that Dec could partly protect against CSE-induced emphysema in mice, enhance Sca-1 expression in lung tissue, and bone marrow-derived EPCs. The results suggested that the depletion of the progenitor cell pool and DNA methylation of Sca-1 gene may be involved in the progression of emphysema in mice.
Intensive Care Unit, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China.
Zhi-Hui
ZH
He
Department of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China Division of Respiratory Disease, Department of Internal Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China pingchen0731@sina.com.
Yan
Y
Chen
Department of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China.
Ping
P
Chen
Department of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China.
Sheng-Dong
SD
He
Department of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China.
Ji-Ru
JR
Ye
Department of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China.
Da
D
Liu
D000818:Animals; D000950:Antigens, Ly / Q000096:biosynthesis*; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000378:metabolism; D002478:Cells, Cultured; D000077209:Decitabine; D004646:Emphysema / Q000139:chemically induced* / Q000473:pathology* / Q000517:prevention & control; D066026:Endothelial Progenitor Cells / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D012906:Smoke; D062789:Tobacco Products; D016896:Treatment Outcome
D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D012906:Smoke; D000077209:Decitabine; D001374:Azacitidine
Cigarette smoke; Decitabine; emphysema; endothelial progenitor cells; stem cell antigen-1