A comparison of the chemical and liver extract-induced hepatic differentiation of adipose derived stem cells.
Adipose-derived stem cells (ADSCs) have been put forward as promising therapeutics for end-stage liver disease (ESLD). In the present study, we compared the effects of defined chemicals and liver extract on the hepatic differentiation of ADSCs. ADSCs were isolated according to the method described in our previously published study. Subsequently, the differentiation of ADSCs was induced separately by chemicals (including hepatic growth factor (HGF), fibroblast growth factor (FGF), and oncostatin M (OSM)) and liver extract (30 μg/ml) in a total period of 21 d. The efficiency of hepatic differentiation was evaluated by changes in the cell morphology, gene expression, and cellular function. The results showed that the liver extract promoted the hepatic differentiation of ADSCs to a significantly greater extent than the chemicals. In the group of ADSCs treated with liver extract, changes in the cell morphology began sooner, and the expression of alpha-FP and albumin genes was higher than that in the chemically treated group. The ADSCs in both the groups stained positive for anti-alpha trypsin (AAT) and albumin markers. The cells also exhibited glycogen storage capacity. Therefore, we concluded that the liver extract could efficiently induce the differentiation of ADSCs into hepatocyte-like cells. This study reveals the potential of mesenchymal stem cell differentiation in the liver extract, which supports further preclinical and clinical research on the application of ADSCs in ESLD treatment.
Laboratory of Stem Cell Research and Application, University of Science, VNU-HCM, HCM City, Vietnam. thnhung@hcmus.edu.vn.|Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City, Vietnam. thnhung@hcmus.edu.vn.
Truong Hai
TH
Nhung
Laboratory of Stem Cell Research and Application, University of Science, VNU-HCM, HCM City, Vietnam. nghnam@hcmus.edu.vn.
Nguyen Hai
NH
Nam
Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City, Vietnam. ntknguyen@hcmus.edu.vn.
Nguyen Thi Kim
NT
Nguyen
University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam. nghiahoathuphuong@gmail.com.
Huynh
H
Nghia
Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam. prnguyenvanthanh@yahoo.com.
Nguyen
N
Van Thanh
Laboratory of Stem Cell Research and Application, University of Science, VNU-HCM, HCM City, Vietnam. pkngoc@hcmus.edu.vn.|Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City, Vietnam. pkngoc@hcmus.edu.vn.
Phan Kim
PK
Ngoc
Laboratory of Stem Cell Research and Application, University of Science, VNU-HCM, HCM City, Vietnam. pvphuc@hcmuns.edu.vn.|Biology Faculty, University of Science, VNU-HCM, Ho Chi Minh City, Vietnam. pvphuc@hcmuns.edu.vn.
Phuc
P
Van Pham
D000273:Adipose Tissue / Q000166:cytology; D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D008107:Liver Diseases / Q000628:therapy*; D008110:Liver Extracts / Q000494:pharmacology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D053683:Oncostatin M / Q000494:pharmacology; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000418:Albumins; C066855:HGF protein, human; D008110:Liver Extracts; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins; D053683:Oncostatin M; D005346:Fibroblast Growth Factors; D017228:Hepatocyte Growth Factor; D006003:Glycogen
Adipose tissue-derived stem cells; Hepatocyte-like cells; Liver tissue extract; Mesenchymal stem cells