Biological and protein-binding studies of newly synthesized polymer-cobalt(III) complexes.
The polymer-cobalt(III) complexes, [Co(bpy)(dien)BPEI]Cl3 · 4H2O (bpy = 2,2'-bipyridine, dien = diethylentriamine, BPEI = branched polyethyleneimine) were synthesized and characterized. The interaction of these complexes with human serum albumin (HSA) and bovine serum albumin (BSA) was investigated under physiological conditions using various physico-chemical techniques. The results reveal that the fluorescence quenching of serum albumins by polymer-cobalt(III) complexes took place through static quenching. The binding of these complexes changed the molecular conformation of the protein considerably. The polymer-cobalt(III) complex with x = 0.365 shows antimicrobial activity against several human pathogens. This complex also induces cytotoxicity against MCF-7 through apoptotic induction. However, further studies are needed to decipher the molecular mode of action of polymer-cobalt(III) complex and for its possible utilization in anticancer therapy.
School of Chemistry, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Vignesh
School of Chemistry, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Pradeep
School of Chemistry, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Arunachalam
Department of Marine Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Vignesh
Department of Marine Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Arthur James
Department of Biomedical Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Arun
Department of Biomedical Science, Bharathidasan University, Tiruchirappalli-, 620 024, Tamil Nadu, India.
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Premkumar
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects*; D001665:Binding Sites; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005658:Fungi / Q000187:drug effects*; D006801:Humans; D061986:MCF-7 Cells; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D009942:Organometallic Compounds; D011108:Polymers; D012709:Serum Albumin; D003035:Cobalt
antimicrobial activity; cytotoxicity; polymer-cobalt(III) complexes; protein binding; static quenching