Robust fluorescence sensing platform for detection of CD44 cells based on graphene oxide/gold nanoparticles.
Gold-coated graphene oxide hybrid material (GO/AuNPs) has exceptional physical and chemical properties like π-π stacking interaction and plays a role in quencher of fluorescence dye. Therefore, GO/AuNPs could enhance the signal-to-background ratio with fluorescence dye that was the point in this fluorescent biosensor. In this study, tetramethyl-6-carboxy-rhodamine (TAMRA)-labeled aptamers that specifically interact with the hyaluronic acid binding domain of CD44 were used as targets to investigate the applicability of the method. GO/AuNPs-TAMRA-aptamer complexes could detect CD44 target cancer cells within a concentration range of 1 × 10(1) to 1 × 10(7) CFU/mL. A linear relationship was observed between target cell concentration and relative fluorescence intensity. The more mounted up CD44 target cell concentrations, relative fluorescence intensity of GO/AuNPs-TAMRA-aptamer complexes was increased even more, which was superior to that of GO alone. Sensitivity of the detection system displayed a low detection limit of 1 × 10(1) CFU/mL. Additionally, this method is specific in that fluorescence is not much enhanced in CD44 negative cancer cell line. Thus, the fluorescence sensing based on GO/AuNPs could be developed to receptive and robust detection tool for various target molecules.
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea.
Ha Young
HY
Jeong
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea.
Seung Hun
SH
Baek
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea.
Sung-Jin
SJ
Chang
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea.
Seon Ah
SA
Cheon
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea. Electronic address: tjpark@cau.ac.kr.
Tae Jung
TJ
Park
D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D014410:Tumor Stem Cell Assay / Q000379:methods
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D018960:Hyaluronan Receptors; D006046:Gold; D006108:Graphite; D006820:Hyaluronic Acid
Aptamer; Fluorescence sensing; Graphene oxide/gold nanoparticles (GO/AuNPs); Hyaluronic acid binding domain of CD44; Signal-to-background ratio enhancer