The elicitor-responsive gene for a GRAS family protein, CIGR2, suppresses cell death in rice inoculated with rice blast fungus via activation of a heat shock transcription factor, OsHsf23.
We show that a rice GRAS family protein, CIGR2, is a bonafide transcriptional activator, and through this function, targets the B-type heat shock protein-encoding gene OsHsf23 (Os09g0456800). CIGR2 (Os07g0583600) is an N-acetylchitooligosaccharide elicitor-responsive gene whose activity, through the direct transcriptional control of OsHsf23, is required for mediating hypersensitive cell death activation during pathogen infection. RNAi lines of CIGR2 and OsHsf23 similarly exhibited the higher level of granulation in the epidermal cells of leaf sheath inoculated with an avirulent isolate of rice blast fungus. Interestingly, we did not observe altered levels of resistance, suggesting that CIGR2 suppresses excessive cell death in the incompatible interaction with blast fungus via activation of OsHsf23.
a Genetically Modified Organism Research Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
Shigeru
S
Tanabe
b Agronomics Research Center, National Institute of Agrobiological Sciences , Tsukuba , Japan.
Haruko
H
Onodera
b Agronomics Research Center, National Institute of Agrobiological Sciences , Tsukuba , Japan.
Naho
N
Hara
a Genetically Modified Organism Research Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
Naoko
N
Ishii-Minami
c Department of Plant, Soil, and Microbial Sciences , Michigan State University , East Lansing , MI , USA.
Brad
B
Day
a Genetically Modified Organism Research Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
Yukiko
Y
Fujisawa
a Genetically Modified Organism Research Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
Takashi
T
Hagio
b Agronomics Research Center, National Institute of Agrobiological Sciences , Tsukuba , Japan.
Seiichi
S
Toki
d Department of Life Sciences , School of Agriculture , Meiji University , Kawasaki , Japan.
Naoto
N
Shibuya
a Genetically Modified Organism Research Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
Yoko
Y
Nishizawa
a Genetically Modified Organism Research Center , National Institute of Agrobiological Sciences , Tsukuba , Japan.
Eiichi
E
Minami
D016923:Cell Death / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D000076249:Heat Shock Transcription Factors; D054884:Host-Pathogen Interactions; D020082:Magnaporthe / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D009844:Oligosaccharides / Q000378:metabolism / Q000494:pharmacology; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; C520160:N-acetylchitooligosaccharide; D009844:Oligosaccharides; D010940:Plant Proteins; D014157:Transcription Factors
GRAS family; cytoplasmic granulation; heat shock transcription factor; rice