Unbiased phosphoproteomic method identifies the initial effects of a methacrylic acid copolymer on macrophages.
An unbiased phosphoproteomic method was used to identify biomaterial-associated changes in the phosphorylation patterns of macrophage-like cells. The phosphorylation differences between differentiated THP1 (dTHP1) cells treated for 10, 20, or 30 min with a vascular regenerative methacrylic acid (MAA) copolymer or a control methyl methacrylate (MM) copolymer were determined by MS. There were 1,470 peptides (corresponding to 729 proteins) that were differentially phosphorylated in dTHP1 cells treated with the two materials with a greater cellular response to MAA treatment. In addition to identifying pathways (such as integrin signaling and cytoskeletal arrangement) that are well known to change with cell-material interaction, previously unidentified pathways, such as apoptosis and mRNA splicing, were also discovered.
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Michael Dean
MD
Chamberlain
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Laura A
LA
Wells
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Alexandra
A
Lisovsky
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
Hongbo
H
Guo
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
Ruth
R
Isserlin
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Ilana
I
Talior-Volodarsky
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Redouan
R
Mahou
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
Andrew
A
Emili
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada M5S 3G9 michael.sefton@utoronto.ca.
Michael V
MV
Sefton
D000327:Adsorption; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects*; D008422:Materials Testing; D008565:Membrane Proteins / Q000378:metabolism; D020366:Methylmethacrylate; D009363:Neoplasm Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D011109:Polymethacrylic Acids / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D040901:Proteomics / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D011109:Polymethacrylic Acids; D014157:Transcription Factors; D020366:Methylmethacrylate; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases
macrophage; material–cell interactions; methacrylic acid; phosphoproteomic