Expression of transferrin in hematoma brain tissue at different stages after intra cerebral hemorrhage in rats.
OBJECTIVE
To explore the expression of transferrin (Tf) and transferrin receptor (TfR) in hematoma brain tissue at different stage after intracerebral hemorrhage (ICH) in rats.


METHODS
ICH rats model were established by collagenase method, and rats were sacrificed at 24 h, 72 h, 7 d and 14 d after operation. The levels of Tf and TfR in different periods of rats were detected by immunohistochemical method, and correlation between two groups was analyzed.


RESULTS
Tf, TfR-positive cells at each time after operation in observation group were significantly higher than that in control group (P < 0.05). Tf, TfR-positive cells began to increase from 24 h after the operation and reached the peak 72 h-7 d after surgery, but then gradually decreased. Tf was mainly expressed in nucleus and cytoplasm of neurons and glial cells around the hematoma, but TfR was mainly expressed in nucleus and cytoplasm of neurons and choroid plexus endothelial cells. Correlation analysis showed that the Tf-positive cell was significantly positively correlated with TfR-positive cell expression (r = 0.447, P = 0.022).


CONCLUSIONS
Tf and TfR were important transporters in brain tissue excessive load iron transport after ICH, and detecting the expression levels of the two indicators can provide a reference for prognosis treatment in ICH.
Department of Neurosurgery, Kunshan Hospital, Nanjing University of Traditional Chinese Medicine, Suzhou 215300, China.
Long
L
Chen
Department of Neurosurgery, Kunshan Hospital, Nanjing University of Traditional Chinese Medicine, Suzhou 215300, China.
Xue-Gang
XG
Jin
Department of Neurosurgery, Kunshan Hospital, Nanjing University of Traditional Chinese Medicine, Suzhou 215300, China.
Jian-Fang
JF
Zhu
Department of Endocrinology, Second People's Hospital, Yangzhou University, Suzhou 205331, China.
Hui-Juan
HJ
Li
Department of Neurosurgery, Huai'An Hospital, Nanjing Medical University, Huai'An 223300, China.
Yan-Ping
YP
Wang
Department of Neurosurgery, First People's Hospital, Suzhou University, Suzhou 215006, China.
You-Xin
YX
Zhou
Department of Neurosurgery, Kunshan Hospital, Nanjing University of Traditional Chinese Medicine, Suzhou 215300, China.
Jian
J
Wang
Department of Neurosurgery, Kunshan Hospital, Nanjing University of Traditional Chinese Medicine, Suzhou 215300, China. Electronic address: chenlong1127a@163.com.
Wen-Hua
WH
Wang
Intracerebral hemorrhage; Transferrin; Transferrin receptor