Light-Driven Hydrogen Production by Hydrogenases and a Ru-Complex inside a Nanoporous Glass Plate under Aerobic External Conditions.
Hydrogenases are powerful catalysts for light-driven H2 production using a combination of photosensitizers. However, except oxygen-tolerant hydrogenases, they are immediately deactivated under aerobic conditions. We report a light-driven H2 evolution system that works stably even under aerobic conditions. A [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F was immobilized inside nanoporous glass plates (PGPs) with a pore diameter of 50 nm together with a ruthenium complex and methyl viologen as a photosensitizer and an electron mediator, respectively. After immersion of PGP into the medium containing the catalytic components, an anaerobic environment automatically established inside the nanopores even under aerobic external conditions upon irradiation with solar-simulated light; this system constantly evolved H2 with an efficiency of 3.7 μmol H2 m(-2) s(-1). The PGP system proposed in this work represents a promising first step toward the development of an O2-tolerant solar energy conversion system.
Tomoyasu
T
Noji
Masaharu
M
Kondo
§Research Institute for Ubiquitous Energy Device, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan.
Tetsuro
T
Jin
∥Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2201, Japan.
Tetsuo
T
Yazawa
⊥Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
Hisao
H
Osuka
⊥Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.|#CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi-shi, Saitama 332-0012, Japan.
Yoshiki
Y
Higuchi
Mamoru
M
Nango
○Center for Gene Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
Shigeru
S
Itoh
Takehisa
T
Dewa
aerobic conditions; artificial photosynthesis; hydrogenase; light-driven hydrogen production; nanoporous glass