Effects of GPNMB on proliferation and odontoblastic differentiation of human dental pulp cells.
Glycoprotein (transmembrane) nonmetastatic melanoma protein b (GPNMB) plays crucial roles in odontogenesis. However, the role of GPNMB in human dental pulp cells (hDPCs) is still unclear. Therefore, in this study, we investigated the expression and function of the GPNMB in odontoblastic differentiation of hDPCs. Cells were cultured in odontoblast differentiation-inducing medium; the expression of the GPNMB was assessed by reverse transcriptase polymerase chain reaction and Western blot analysis. We performed gene knockdown of GPNMB in hDPCs using lentivirus-mediated small interfering RNA (siRNA)-GPNMB. The proliferation of cells was measured by the MTT assay, and the differentiation of cells was detected with alkaline phosphatase (ALP) activity assay, qRT-PCR and Western blot were used to determine the expression levels of dentin sialophosphoprotein (DSPP) and dentin matrix protein-1 (DMP-1). The expression level of GPNMB was significantly increased during odontoblastic differentiation of hDPCs. Suppression of GPNMB expression by siRNA-GPNMB obviously promoted the proliferation of hDPCs. Furthermore, siRNA-GPNMB significantly inhibited the activity of ALP and expression levels of DSPP and DMP-1 during odontoblastic differentiation of hDPCs. Our results show that GPNMB plays an important role in regulating the expression of key pluripotency genes in hDPCs and modifying odontogenic differentiation.
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University Jinan 250012, China ; Department of Oral and Maxillofacial Surgery, Yantai Affiliated Hospital of Binzhou Medical University Yantai 264117, China.
Yu-Liang
YL
Wang
Department of Pathophysiology, Binzhou Medical University Binzhou 264003, China.
Ye-Jia
YJ
Hu
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University Jinan 250012, China.
Feng-He
FH
Zhang
D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009804:Odontoblasts / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
D015415:Biomarkers; C503042:GPNMB protein, human; D008562:Membrane Glycoproteins
Glycoprotein (transmembrane) nonmetastatic melanoma protein b (GPNMB); human dental pulp cells (hDPCs); odontoblastic differentiation; proliferation