Application of 3D NMR for Structure Determination of Peptide Natural Products.
Despite the advances in NMR, structure determination is often slow and constitutes a bottleneck in natural products discovery. Removal of this bottleneck would greatly improve the throughput for antibiotic discovery as well as other therapeutic areas. Overall, faster structure methods for structure determination will serve the natural products community in a broad manner. This report describes the first application of 3D NMR for elucidation of two microbially produced peptide natural products with novel structures. The methods are cost-effective and greatly improve the confidence in a proposed structure.
Pharmaceutical Sciences Division, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
Fan
F
Zhang
Pharmaceutical Sciences Division, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
Navid
N
Adnani
Molecular & Environmental Toxicology Center, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
Emmanuel
E
Vazquez-Rivera
Pharmaceutical Sciences Division, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
Doug R
DR
Braun
National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Marco
M
Tonelli
Department of Medicine, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
David R
DR
Andes
Pharmaceutical Sciences Division, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
Tim S
TS
Bugni
D001688:Biological Products / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*
D001688:Biological Products; D010455:Peptides