Local blockage of EMMPRIN impedes pressure ulcers healing in a rat model.
Excessive extracellular matrix degradation caused by the hyperfunction of matrix metalloproteinases (MMPs) has been implicated in the failure of pressure ulcers healing. EMMPRIN, as a widely expressed protein, has emerged as an important regulator of MMP activity. We hypothesize that EMMPRIN affects the process of pressure ulcer healing by modulating MMP activity. In the rat pressure ulcer model, the expression of EMMPRIN in ulcers detected by Western blot was elevated compared with that observed in normal tissue. To investigate the role of EMMPRIN in regulating ulcer healing, specific antibodies against EMMPRIN were used via direct administration on the pressure ulcer. Local blockage of EMMPRIN resulted in a poor ulcer healing process compared with control ulcers, which was the opposite of our expectation. Furthermore, inhibiting EMMPRIN minimally impacted MMP activity. However, the collagen content in the pressure ulcer was reduced in the EMMPRIN treated group. Angiogenesis and the expression of angiogenic factors in pressure ulcers were also reduced by EMMPRIN local blockage. The results in the present study indicate a novel effect of EMMPRIN in the regulation of pressure ulcer healing by controlling the collagen contents and angiogenesis rather than MMPs activity.
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Xi-Lan
XL
Zhao
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Xiao
X
Luo
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Ze-Xin
ZX
Wang
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Guo-Li
GL
Yang
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Ji-Zhong
JZ
Liu
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Ya-Qiong
YQ
Liu
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Ming
M
Li
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Min
M
Chen
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Yong-Mei
YM
Xia
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Jun-Jie
JJ
Liu
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Shu-Ping
SP
Qiu
Department of Nursing, Fuling Central Hospital Chongqing 408099, China.
Xiao-Qing
XQ
Gong
D042501:Angiogenic Proteins / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D051926:Basigin / Q000276:immunology / Q000378:metabolism; D001798:Blood Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D018919:Neovascularization, Physiologic; D003668:Pressure Ulcer / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D042501:Angiogenic Proteins; D000906:Antibodies; D001798:Blood Proteins; C079432:Bsg protein, rat; D051926:Basigin; D003094:Collagen; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat
CD147; EMMPRIN; Pressure ulcer; angiogenesis; collagen; matrix metalloproteinases