Testis follicles ultrastructure of three species of terrestrial isopods (Crustacea, Isopoda Oniscidea).
The aim of the research, carried out on three species of terrestrial isopods - Armadillidium granulatum, Halophiloscia hirsuta and Trichoniscus alexandrae - is to bring a first consistent contribution to the knowledge of the ultrastructural organization of the testis follicles. The testis follicles are seat of a remarkable dynamic activity of their cell components (somatic cells and germ cells) that results in a continuous variation, related to the trend of spermatogenesis, of their morphology, organization and of the relationships between the two cell populations. The somatic cells, known in literature as follicular cells, nurse cells or Sertoli cells, are arranged at the periphery of the follicle to form an epithelial layer of variable thickness resting on a thin basal lamina in turn surrounded by a discontinuous network of muscle cells. In A. granulatum and H. hirsuta, two types of Sertoli cells are present: a first type, the nurse cells, envelop the spermatids in cavities within their cytoplasm and through their secretion activity play a fundamental role in the formation of the spermatophores; moreover, they phagocytizes the residual cytoplasm of spermatids. A second type of Sertoli cells shows features that leave clearly identify its supporting role to the spermatophores in formation. In T. alexandrae, instead, only one type of Sertoli cells, the nurse cell, is present, whose features are widely superimposable to those observed in the other two species. Moreover, two septa of Sertoli cells depart from the periphery of the testis follicle to constitute an articulated compartmentalization of the follicle itself, probably targeted to realize at its inside a series of microenvironments functionally diversified in order to meets the needs of the different stages of the spermatogenic cycle.
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università di Catania, Italy. Electronic address: vmazzei@unict.it.
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Mazzei
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università di Catania, Italy.
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Longo
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università di Catania, Italy.
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Brundo
D000818:Animals; D003445:Crustacea / Q000648:ultrastructure*; D018859:Hair Follicle / Q000648:ultrastructure; D033321:Isopoda / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D012708:Sertoli Cells / Q000648:ultrastructure*; D013091:Spermatogenesis / Q000502:physiology; D013093:Spermatogonia / Q000648:ultrastructure*; D013737:Testis / Q000648:ultrastructure*
Phagocytosis; Sertoli cell; Spermatogenesis; Spermatophore; Ultrastructure