Separation of Y-chromosomal haplotypes from male DNA mixtures via multiplex haplotype-specific extraction.
In forensic analysis, the interpretation of DNA mixtures is the subject of ongoing debate and requires expertise knowledge. Haplotype-specific extraction (HSE) is an alternative method that enables the separation of large chromosome fragments or haplotypes by using magnetic beads in conjunction with allele-specific probes. HSE thus allows physical separation of the components of a DNA mixture. Here, we present the first multiplex HSE separation of a Y-chromosomal haplotype consisting of six Yfiler short tandem repeat markers from a mixture of male DNA.
Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité- Campus Virchow-Klinikum, Augustenburger Platz 1, Forum 4, 13353 Berlin, Germany. Electronic address: jessica.rothe@charite.de.
Jessica
J
Rothe
Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité- Campus Virchow-Klinikum, Augustenburger Platz 1, Forum 4, 13353 Berlin, Germany.
Marion
M
Nagy
D041322:Chromosomes, Human, Y; D004247:DNA / Q000235:genetics*; D053803:Forensic Genetics; D006239:Haplotypes; D006801:Humans; D008297:Male
D004247:DNA
DNA mixtures; HSE; Haplotype specific extraction