Genetic diversity and population structure of Synthesium pontoporiae (Digenea, Brachycladiidae) linked to its definitive host stocks, the endangered Franciscana dolphin, Pontoporia blainvillei (Pontoporiidae) off the coast of Brazil and Argentina.
Pontoporia blainvillei (Gervais and d'Orbigny, 1844) is an endangered small cetacean endemic to South America with four Franciscana Management Areas (FMA) recognized as different population stocks. The role of the intestinal parasite Synthesium pontoporiae (Digenea: Brachycladiidae) as a possible biological marker to differentiate P. blainvillei stocks was evaluated using nuclear and mitochondrial DNA markers. Internal transcribed sequence 1 and 2 (ITS1 and ITS2) regions of S. pontoporiae did not show intraspecific variability. The mitochondrial NADH dehydrogenase subunit 3 (ND3) and cytochrome oxidase subunit I (COI) gene sequences suggested lack of population structure in S. pontoporiae and population expansion. The apparent panmixia of S. pontoporiae may be due to the high mobility of one or more of its intermediary hosts. Alternatively, it may be due to the small sample size. This result is incongruent with the previously proposed FMA.
Laboratório de Genética Molecular de Microorganismos,Instituto Oswaldo Cruz, FIOCRUZ,Rio de Janeiro,RJ,Brazil.
J
J
Marigo
Laboratório de Biodiversidade Molecular (UFRJ)/Laboratório de Mamíferos Aquáticos e Bioindicadores (MAQUA, UERJ),Rio de Janeiro,RJ,Brazil.
H A
HA
Cunha
Projeto BioPesca,Praia Grande,SP,Brazil.
C P
CP
Bertozzi
Instituto Terra & Mar,Ilhabela,SP,Brazil.
S P
SP
Souza
Instituto Nacional de Pesquisas da Amazônia,Manaus,AM,Brazil.
F C W
FC
Rosas
Universidade da Região de Joinville,Joinville,SC,Brazil.
M J
MJ
Cremer
Universidade do Vale do Itajaí,Balneário Camboriú,SC,Brazil.
A S
AS
Barreto
Laboratório de Ecologia de Mamíferos Universidade do Vale do Rio dos Sinos (UNISINOS), Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS),Porto Alegre,RS,Brazil.
L R
LR
de Oliveira
Museo Argentino de Ciencias Naturales 'Bernardino Rivadavia' (MACN-CONICET)/Universidad Maimónides y Fundación Azara, CABA,Buenos Aires,Argentina.
H L
HL
Cappozzo
Universidade Federal de Pelotas,Pelotas,RS,Brazil.
A L S
AL
Valente
Laboratório de Avaliação e Promoção da Saúde Ambiental,Instituto Oswaldo Cruz, FIOCRUZ,Rio de Janeiro,RJ,Brazil.
C P
CP
Santos
Laboratório de Genética Molecular de Microorganismos,Instituto Oswaldo Cruz, FIOCRUZ,Rio de Janeiro,RJ,Brazil.
A C P
AC
Vicente
D000818:Animals; D001118:Argentina; D001938:Brazil; D002590:Cestode Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004289:Dolphins / Q000469:parasitology*; D003576:Electron Transport Complex IV / Q000235:genetics; D056727:Endangered Species; D014644:Genetic Variation; D015801:Helminth Proteins / Q000235:genetics; D007411:Intestinal Diseases, Parasitic / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D010986:Platyhelminths / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*
D015801:Helminth Proteins; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV