[Screening and functional analysis of microRNA expression in HPV16-positive squamous carcinoma of the cervix  in the Uygur of southern Xinjiang].
OBJECTIVE
To explore the differential expression of microRNA (miRNA) in HPV16-positive squamous carcinoma of the cervix in the Uygur of southern Xinjiang and to predict the target genes of the miRNAs.


METHODS
Samples of HPV16-positive squamous carcinoma of the cervix from 5 Uygurs were collected for miRNA microarray assay. The differentially expressed miRNAs were selected for further verification by real-time quantitative RT-PCR. The software, including targetscan, miRwalk, miRanda and pictar, were used to predict the target genes of the verified miRNAs.


RESULTS
Eighteen differentially expressed miRNAs were identified by miRNA microarray assay. The significantly differentially expressed miRNA-138 and miRNA-720 were verified by real-time quantitative RT-PCR. According to the prediction, the target genes for miRNA-138 were EZH2, LYPLA1, ARHGEF3, CLNS1A, EIF4EBP1, GNAI2, LIMK1, RHOC, ROCK2, SLC20A1, TERT, and H2AFX, while for miRNA-720 were EZH2, AGAP2, SPOCK2, FGF14, HNRNPA2B1, QKI, FOXG1, ACVR1B, DNMT3A, EPHB2, LATS2, KRAS, CCND2, NBN, ENAM, AMELX, PRNP, and CALB1.


CONCLUSIONS
miR-138 and miR-720 are the down-regulated target miRNAs in HPV16-positive squamous carcinoma of the cervix in the Uygur of southern Xinjiang. The common target gene for miR-138 and miR-720 is EZH2, which might be related to cervical squamous carcinoma invasion and metastasis.
Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Ulumuqi 830011, China.
Min
M
Yuan
Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Ulumuqi 830011;Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
Jingxin
J
Cheng
Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Ulumuqi 830011, China.
Yaxin
Y
Liu
Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Ulumuqi 830011, China.
Wei
W
Su
Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
Yu
Y
Zhang
Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
Yi
Y
Zhang
D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000821:virology*; D002681:China; D015536:Down-Regulation; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000821:virology*
D035683:MicroRNAs