Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera.
The canine monocytic ehrlichiosis, caused by Ehrlichia canis, is endemic in several regions of Brazil. Some serological diagnostic techniques using immunodominant proteins of E. canis as antigens are available, but their specificities and sensitivities are questionable. Based on this, the objective of this study was to test the antigenic potential of the recombinant gp19 protein (rGP19) for subsequent use in diagnostic tests. The rGP19 expressed in the Escherichia coli strain BL21 (DE3) C41 was recognized in the sera from experimentally infected dogs using ELISA and Western blotting. Thus, it was possible to obtain a promising antigen with the ability to differentiate between E. canis-positive and -negative animals, even 1 week after infection.
Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas, Pelotas, RS, BR.
Rômulo Silva de
RS
Oliveira
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BR.
Rodrigo Casquero
RC
Cunha
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BR.
Jonas
J
Moraes-Filho
Pós-graduação em Biologia Celular e Molecular, Universidade de São Paulo, São Paulo, SP, BR.
Relber Aguiar
RA
Gonçales
Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas, Pelotas, RS, BR.
Ana Paula de Souza Stori de
AP
Lara
Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas, Pelotas, RS, BR.
Luciana Farias da Costa de
LF
Avila
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BR.
Marcelo Bahia
MB
Labruna
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BR.
Fábio Pereira Leivas
FP
Leite
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001938:Brazil; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis*; D004285:Dogs; D041061:Ehrlichia canis / Q000276:immunology*; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011994:Recombinant Proteins