Skin donors and human skin allografts: evaluation of an 11-year practice and discard in a referral tissue bank.
The Saint Louis hospital tissue bank provides skin allografts to pediatric and adult burn units in the Paris area. The aim of this study was to analyze our activity during the last 11 years focusing on the reasons for skin discard. Skin is procured solely from the back of the body, which is divided into 10 zones that are harvested and processed separately. This retrospective study included all skin donors harvested between June 2002 and June 2013, representing a total of 336 donors and 2770 zones. The donors were multiorgan heart-beating donors in 91 % of cases (n = 307). The main reason for discarding harvested skin was microbial contamination, detected in 99 donors (29 %). Most contaminants were of low pathogenicity. Other reasons for discard included positive serologic tests for 2 donors [17 zones (0.61 %)], unsuitable physical skin characteristics for 3 zones (0.11 %), the donor's medical history for 53 zones (1.91 %), and technical issues with processing or distribution for 61 zones (2.2 %). In our experience, microbial contamination continues to be the main reason for discarding potential skin allografts. However, discards are limited by separate harvesting and processing of multiple zones in each donor.
Faculté de Médecine, Université Paris Descartes, Paris, France.|Service de Chirurgie Générale, Plastique et Ambulatoire, APHP, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Paris, France.
Sonia
S
Gaucher
Banque des Tissus Humains, APHP, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75 010, Paris, France.
Zena
Z
Khaznadar
Banque des Tissus Humains, APHP, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75 010, Paris, France.
Jean-Claude
JC
Gourevitch
Banque des Tissus Humains, APHP, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75 010, Paris, France. mohamed.jarraya@sls.aphp.fr.
Mohamed
M
Jarraya
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D064591:Allografts / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification; D002423:Cause of Death; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012017:Referral and Consultation; D016038:Skin Transplantation; D014015:Tissue Banks; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting; D055815:Young Adult
Discards; Microbial contamination; Skin allografts; Skin donors; Tissue banking