Characterization of Brassica napus Flavonol Synthase Involved in Flavonol Biosynthesis in Brassica napus L.
Recently, Brassica napus has become a very important crop for plant oil production. Flavonols, an uncolored flavonoid subclass, have a high antioxidative effect and are known to have antiproliferative, antiangiogenic, and neuropharmacological properties. In B. napus, some flavonoid structural genes have been identified, such as, BnF3H-1, BnCHS, and BnC4H-1. However, no studies on FLS genes in B. napus have been conducted. Thus, in this study, we cloned and characterized the function of BnFLS gene B. napus. By overexpression of the BnFLS gene, flavonol (kaempferol and quercetin) levels were recovered in the Arabidopsis atfls1-ko mutant. In addition, we found that the higher endogenous flavonol levels of BnFLS-ox in vitro shoots correlated with slightly higher ROS scavenging activities. Thus, our results indicate that the BnFLS gene encodes for a BnFLS enzyme that can be manipulated to specifically increase flavonol accumulation in oilseed plants and other species such as Arabidopsis.
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
Tien Thanh
TT
Vu
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.|Institute of Life Science and Natural Resources, Korea University , Seoul 136-713, Republic of Korea.
Chan Young
CY
Jeong
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.|Institute of Life Science and Natural Resources, Korea University , Seoul 136-713, Republic of Korea.
Hoai Nguyen
HN
Nguyen
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
Dongho
D
Lee
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
Sang A
SA
Lee
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.
Ji Hye
JH
Kim
Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University , Gwangju, Republic of Korea.
Suk-Whan
SW
Hong
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea.|Institute of Life Science and Natural Resources, Korea University , Seoul 136-713, Republic of Korea.
Hojoung
H
Lee
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029688:Brassica napus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D044948:Flavonols / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D044948:Flavonols; D010940:Plant Proteins; D010088:Oxidoreductases; C085313:flavonol synthase
BnFLS; Brassica napus; anthocyanin; flavonoids; flavonols; oilseed