Structural Characterization of Ginsenosides from Flower Buds of Panax ginseng by RRLC-Q-TOF MS.
Ginseng flower bud as a part of Panax ginseng has received much attention as a valuable functional food with medicinal potential. A few studies focused on systematic and comprehensive studies on its major ingredients. This study aims to rapidly characterize ginsenosides in ginseng flower buds and provide scientific basis for developing functional food, exploiting pharmaceutical effects and making full use of ginseng resources. A rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method was developed for rapid qualitative and quantitative analysis of ginsenosides in ginseng flower buds. The compounds were identified by comparing retention time of the reference standards, accurate mass measurement and the fragment ions obtained from RRLC-Q-TOF-MS/MS analyses. A total of 14 kinds of ginsenosides were identified and 5 kinds of malonyl-ginsenosides were first tentatively identified in ginseng flower buds. Ten kinds of main ginsenosides were quantitatively analyzed. The developed RRLC-Q-TOF-MS method was demonstrated as an effective analytical means for rapid characterization of the ginsenosides in flower buds of P. ginseng. The research result is valuable for quality control, assessment of authenticity and stability evaluation of ginseng flower buds.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun 130117, China.
Wei
W
Wu
Jilin Ginseng Academy, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun 130117, China.
Ziyan
Z
Lu
Jilin Ginseng Academy, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun 130117, China.
Yaran
Y
Teng
Jilin Province Product Quality Supervision Test Institute, Changchun 130103, China.
Yingying
Y
Guo
Jilin Ginseng Academy, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun 130117, China Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China syliu@ciac.jl.cn.
Shuying
S
Liu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D036145:Ginsenosides / Q000737:chemistry*; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D036145:Ginsenosides; D010936:Plant Extracts