Changes of soluble CD40 ligand in the progression of acute myocardial infarction associate to endothelial nitric oxide synthase polymorphisms and vascular endothelial growth factor but not to platelet CD62P expression.
Reported in vitro data implicated soluble CD40 ligand (sCD40L) in endothelial dysfunction and angiogenesis. However, whether sCD40L could exert that influence in endothelial dysfunction and angiogenesis after injury in acute myocardial infarction (AMI) patients remains unclear. In the present study, we evaluated the association of sCD40L with markers of platelet activation, endothelial, and vascular function during a recovery period early after AMI. To achieve this goal, the time changes of soluble, platelet-bound, and microparticle-bound CD40L levels over 1 month were assessed in AMI patients and correlated with endothelial nitric oxide synthase (eNOS) polymorphisms, vascular endothelial growth factor (VEGF) concentrations, and platelet expression of P-selectin (CD62P). The association of soluble form, platelet-bound, and microparticle-bound CD40L with CD62P expression on platelets, a marker of platelet activation, was also assessed to evaluate the role of CD40L in the thrombosis, whereas the association with eNOS and VEGF was to evaluate the role of CD40L in vascular dysfunction. This work shows for the first time that time changes of sCD40L over 1 month after myocardial infarct onset were associated with G894T eNOS polymorphism and with the VEGF concentrations, but not to the platelet CD62P expression. These results indicate that, in terms of AMI pathophysiology, the sCD40L cannot be consider just as being involved in thrombosis and inflammation but also as having a relevant role in vascular and endothelial dysfunction.
Carlota Saldanha Lab, Instituto Medicina Molecular (iMM), Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal. Electronic address: pnapoleao@medicina.ulisboa.pt.
Patrícia
P
Napoleão
IINFACTS-CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Penafiel, Portugal.
Maria do Céu
Mdo C
Monteiro
IINFACTS-CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Penafiel, Portugal.
Luís B P
LB
Cabral
IINFACTS-CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Penafiel, Portugal.
Maria Begoña
MB
Criado
Departamento de Engenharia e Ciências Nucleares, Instituto de Bioengenharia e Biociências (IBB), Instituto Superior Técnico, Universidade de Lisboa, Portugal.
Catarina
C
Ramos
Serviço Cardiologia, Hospital Santa Marta, Centro Hospitalar Lisboa Central (CHLC), Lisboa, Portugal.
Mafalda
M
Selas
Centro de Estudos do Ambiente e do Mar (CESAM) & Departamento de Biologia Animal (DBA), Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal.
Ana Maria
AM
Viegas-Crespo
Carlota Saldanha Lab, Instituto Medicina Molecular (iMM), Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal.
Carlota
C
Saldanha
Serviço Cardiologia, Hospital Santa Marta, Centro Hospitalar Lisboa Central (CHLC), Lisboa, Portugal; Centro de Estudos de Doenças Crónicas (CEDOC), Faculdade Ciências Médias, Universidade Nova de Lisboa, Lisboa, Portugal.
Miguel Mota
MM
Carmo
Serviço Cardiologia, Hospital Santa Marta, Centro Hospitalar Lisboa Central (CHLC), Lisboa, Portugal.
Rui Cruz
RC
Ferreira
Departamento de Engenharia e Ciências Nucleares, Instituto de Bioengenharia e Biociências (IBB), Instituto Superior Técnico, Universidade de Lisboa, Portugal.
Teresa
T
Pinheiro
D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D019007:P-Selectin / Q000235:genetics*; D011110:Polymorphism, Genetic; D011993:Recombinant Fusion Proteins / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
C526581:CD40LIg fusion protein; D019007:P-Selectin; D011993:Recombinant Fusion Proteins; C546937:SELP protein, human; D042461:Vascular Endothelial Growth Factor A; D052250:Nitric Oxide Synthase Type III