Application of Gelatin-Coated Magnetic Particles for Isolation of Genomic DNA from Bones.
OBJECTIVE
To develop a method for human genomic DNA extraction from bone using gelatin-coated magnetic particles.


METHODS
Thirty human metacarpal with the bone age ranging from 36 to 93 years were included in the present study. Genomic DNA was extracted from bones using gelatin-coated magnetic particles. The concentration and purity of DNA were analyzed in comparison with a reference method. In addition, the quality of extracted DNA was examined for sex determination by conventional polymerase chain reaction (PCR).


RESULTS
The average DNA concentration using gelatin coated magnetic particles exhibited approximately 15 times higher than a reference method with an insignificantly difference of the DNA purity in both methods. Twelve (40%) and fifteen (50%) samples out of thirty DNA isolated using established and reference method, respectively, could be amplified and sex correctly determined by PCR.


CONCLUSIONS
Gelatin coated magnetic particle is rapid, simple, and well-suited for isolation of DNA from bones.
Pongsak
P
Khanpetch
Sorasak
S
Intorasoot
Sukon
S
Prasitwattanseree
Karnda
K
Mekjaidee
Pasuk
P
Mahakkanukrauh
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001842:Bone and Bones / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification*; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D059346:Magnets; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
D005780:Gelatin; D004247:DNA