The 811 C/T polymorphism in the 3' untranslated region of the selenoprotein 15-kDa (Sep15) gene and breast cancer in Caucasian women.
The 15-kDa selenoprotein (Sep15) is a selenocysteine-containing oxidoreductase in the endoplasmic reticulum that participates in disulfide-bond formation and protein folding control. The 3'-untranslated region (3'-UTR) contains two exclusively linked, polymorphic sites at positions 811 (C/T) and 1125 (G/A), which result in two functional haplotypes: 811C/1125G or 811T/1125A. The 811T/1125A variant occurs significantly more often in African-Americans as compared to Caucasians and has been linked to increased breast cancer risk in black women. We studied the 811C/T (rs5845) Sep15 gene polymorphism in 182 Caucasian women-83 breast cancer cases and 99 healthy controls-by pyrosequencing and polymerase chain reaction. Associations between allelic variants and clinico-pathological variables (e.g., age, stage of disease, tumor type, grading, and receptor status) were investigated. The genotype distribution in breast cancer patients (CC 63.9 %, CT 33.7 %, TT 2.4 %) and controls (69.7 %, CT 28.3 %, TT 2 %) showed no significant difference (OR 0.77, 95 % CI 0.41-1.42, p = 0.4). The overall low prevalence of the T allele was in accordance with that reported for Caucasians in previous studies. There was no significant association between 811C/T Sep15 polymorphism and any of clinico-pathological parameters. In conclusion, we are the first to report on 811C/T SEP 15 polymorphism in white breast cancer patients. Genotype variation within the 3'-UTR of the SEP 15 gene showed no association with breast cancer risk or clinico-pathological parameters in Caucasian women.
Department of Gynecology and Obstetrics, St. Josefskrankenhaus, Teaching Hospital of the University of Freiburg, Sautierstr. 1, 79104, Freiburg, Germany. Rafal.Watrowski@gmx.at.|Department of Obstetrics and Gynecology, Molecular Oncology Group, Medical University of Vienna, Ebene 5Q, Währinger Gürtel 18-20, 5Q, 1090, Vienna, Austria. Rafal.Watrowski@gmx.at.
Rafał
R
Watrowski
Department of Obstetrics and Gynecology, Molecular Oncology Group, Medical University of Vienna, Ebene 5Q, Währinger Gürtel 18-20, 5Q, 1090, Vienna, Austria.
Dan Cacsire
DC
Castillo-Tong
Department of Obstetrics and Gynecology, Molecular Oncology Group, Medical University of Vienna, Ebene 5Q, Währinger Gürtel 18-20, 5Q, 1090, Vienna, Austria.
Gerhild
G
Fabjani
Department of Obstetrics and Gynecology, Molecular Oncology Group, Medical University of Vienna, Ebene 5Q, Währinger Gürtel 18-20, 5Q, 1090, Vienna, Austria.
Eva
E
Schuster
Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
Michael
M
Fischer
Department of Obstetrics and Gynecology, Molecular Oncology Group, Medical University of Vienna, Ebene 5Q, Währinger Gürtel 18-20, 5Q, 1090, Vienna, Austria.
Robert
R
Zeillinger
D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D000483:Alleles; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D051140:Selenoproteins / Q000235:genetics*; D044465:White People / Q000235:genetics*
D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; C495238:SELENOF protein, human; D051140:Selenoproteins
Breast cancer; Polymorphism; Selenoprotein 15; Selenoproteins; Sep15