CRISPR EATING on a Low Budget.
In this issue of Developmental Cell, Lane et al. (2015) describe a simplified, inexpensive technique named "CRISPR EATING" for generating complex guide RNA libraries suitable for CRISPR/Cas9-based applications ranging from genome visualization and manipulation to genetic screens.
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Hatice S
HS
Kaya-Okur
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: asbel@illinois.edu.
Andrew S
AS
Belmont
D000818:Animals; D001426:Bacterial Proteins; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004720:Endonucleases; D015723:Gene Library; D012313:RNA / Q000235:genetics*
D001426:Bacterial Proteins; D012313:RNA; D004720:Endonucleases