Protection of seven dibenzocyclooctadiene lignans from Schisandra chinensis against serum and glucose deprivation injury in SH-SY5Y cells.
Dibenzocyclooctadiene lignans, the major active components of fruit of Schisandra chinensis (Turcz.) Baill., have been found to have activities that could prevent prostate and thyroid cancer, hepatotoxicity, oxidative stress-induced cerebral injury, etc. This study was conducted to evaluate the effects of seven dibenzocyclooctadiene lignans of Schisandra chinensis and explore the possible mechanisms in the human neuroblastoma SH-SY5Y cells exposed on serum and glucose deprivation (SGD) injury. The structure-activity relationships were also analyzed. Cell viability and lactate dehydrogenase (LDH) release were determined to evaluate cell injury. Inflammation and apoptosis-related protein levels were detected to elucidate the possible mechanisms. Schisantherin A, schizandrin C, and schizandrol B were found to have stronger protective effects than schizandrin A, schizandrin B, and schisanhenol in SH-SY5Y cells against SGD injury. Moreover, the protective effects of these lignans were possibly exhibited by regulating inflammation and apoptosis-related proteins in SH-SY5Y cells after SGD injury, supporting their beneficial effects for the prevention of cell injury in the pathogenesis of the central nervous system diseases, including ischemia stroke. The number and position of hydroxyl group and methylenedioxy in these lignans may be required for their effects.
Department of Pathology, School of Medicine, Nantong University, Nantong, 226001, P. R. China.
Qun
Q
E
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
Miao
M
Tang
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
XiaoChuan
X
Zhang
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
YunWei
Y
Shi
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
DanDan
D
Wang
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
Yun
Y
Gu
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
ShiYing
S
Li
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, 116023, P. R. China.
XinMiao
X
Liang
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Department of Pharmacology, University of California, Irvine, California, 92697, USA.
ZhiWei
Z
Wang
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.|Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
CaiPing
C
Wang
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free / Q000633:toxicity*; D034242:Cyclooctanes / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency*; D006801:Humans; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D032141:Schisandra
D016895:Culture Media, Serum-Free; D034242:Cyclooctanes; D017705:Lignans; C510994:dibenzocyclooctadiene lignan; D005947:Glucose
Schisandra chinensis; apoptosis; dibenzocyclooctadiene lignans; inflammation; neuroprotection