Improved Protocols for Illumina Sequencing.
In this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom.
Iraad F
IF
Bronner
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom.
Michael A
MA
Quail
Oxford Nanopore Technologies, Oxford, United Kingdom.
Daniel J
DJ
Turner
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom.
Harold
H
Swerdlow
D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000592:standards; D009691:Nucleic Acid Denaturation
Illumina; next-generation; protocols; sequencer