Effects of β-hydroxybutyricacid on the synthesis and assembly of very low-density lipoprotein in bovine hepatocytes in vitro.
β-Hydroxybutyricacid (BHBA) is an important metabolite that involved in the development of ketosis and fatty liver in dairy cows. Dairy cows with fatty liver displayed high blood concentration of BHBA and very low-density lipoprotein (VLDL) assembly. The effects of BHBA on VLDL synthesis and assembly in hepatocytes of cows were unclear. In this study, bovine hepatocytes were cultured and treated with different concentrations of BHBA. We found that BHBA treatment upregulated the mRNA and protein levels of apolipoprotein B100 (ApoB 100), apolipoprotein E (ApoE) and microsomal triglyceride transfer protein (MTTP) and showed in a firstly increased and then decreased trend. Meanwhile, the mRNA and protein levels of LDLR showed in a reverse trend. Consequently, VLDL content was significantly increased in medium-dose BHBA treatment group, while decreased in high-dose group. These results indicate that the effects of BHBA on the VLDL synthesis showed in a dose-dependent manner that low levels of BHBA increase VLDL synthesis and high levels of BHBA decrease VLDL synthesis.
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.|College of Animal Science and Technology, Inner Mongolia National University, Tongliao, China.
Q
Q
Deng
College of Animal Science and Technology, Inner Mongolia National University, Tongliao, China.
D
D
Ma
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
Z
Z
Shi
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
W
W
Huang
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
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X
Du
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
W
W
Gao
UMR 788, Inserm and University Paris-Sud, Bicêtre, Kremlin-Bicêtre, France.
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X
Zhu
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
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L
Lei
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
M
M
Zhang
College of Animal Science and Technology, Inner Mongolia National University, Tongliao, China.
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G
Sun
College of Animal Science and Technology, Inner Mongolia National University, Tongliao, China.
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X
Yuan
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
X
X
Li
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
Z
Z
Wang
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.|College of Animal Science and Technology, Inner Mongolia National University, Tongliao, China.
G
G
Liu
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, Jilin, China.
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X
Li
D020155:3-Hydroxybutyric Acid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D015243:Cholesterol, VLDL / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism
D015243:Cholesterol, VLDL; D020155:3-Hydroxybutyric Acid
bovine hepatocyte; negative energy balance; synthesis and assembly; very low-density lipoprotein; β-Hydroxybutyricacid