Facile synthesis of partially uncapped liposomes.
Sophisticated control of the assembly of nano-sized structures to achieve desired properties is one of the most efficient techniques in drug carrier design. In this study, we hypothesized that under magnetic shear stress, high-density superparamagnetic Fe3O4 nanoparticles dispersed in a liposome would apply a high load in a particular direction to a liposome membrane and ultimately generate open lipid bilayer holes. Indeed, the designed experimental conditions enabled the formation of one or multiple open pore sites in liposome membranes. With this evidence, open lipid bilayer holes in liposome membranes were further used as open entrances for proteins (bovine serum albumin or insulin) into the liposomes prior to the natural recovery (i.e., closing) of the lipid bilayer holes. This trial will provide potential opportunities for the engineering of functional liposomes for protein drug delivery.
Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon, Gyeonggi-do 420-743, Republic of Korea.
Dong Sup
DS
Kwag
Division of Bio-imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, Republic of Korea.
Kyeongsoon
K
Park
School of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon, Gyeonggi-do 440-746, Republic of Korea.
Yu Seok
YS
Youn
Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon, Gyeonggi-do 420-743, Republic of Korea. Electronic address: eslee@catholic.ac.kr.
Eun Seong
ES
Lee
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004355:Drug Stability; D004574:Electromagnetic Fields; D005290:Ferric Compounds / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D008051:Lipid Bilayers; D008081:Liposomes / Q000138:chemical synthesis*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008567:Membranes, Artificial; D053758:Nanoparticles; D010316:Particle Size; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005290:Ferric Compounds; D007328:Insulin; D008051:Lipid Bilayers; D008081:Liposomes; D058185:Magnetite Nanoparticles; D008567:Membranes, Artificial; C000499:ferric oxide; D012710:Serum Albumin, Bovine
Closed pore; Fe(3)O(4); Magnetic shear stress; Partially uncapped liposome