Stem Cell-Soluble Signals Enhance Multilumen Formation in SMG Cell Clusters.
Saliva plays a major role in maintaining oral health. Patients with salivary hypofunction exhibit difficulty in chewing and swallowing foods, tooth decay, periodontal disease, and microbial infections. At this time, treatments for hyposalivation are limited to medications (e.g., muscarinic receptor agonists: pilocarpine and cevimeline) that induce saliva secretion from residual acinar cells as well as artificial salivary substitutes. Therefore, advancement of restorative treatments is necessary to improve the quality of life in these patients. Our previous studies indicated that salivary cells are able to form polarized 3-dimensional structures when grown on growth factor-reduced Matrigel. This basement membrane is rich in laminin-III (L1), which plays a critical role in salivary gland formation. Mitotically inactive feeder layers have been used previously to support the growth of many different cell types, as they provide factors necessary for cell growth and organization. The goal of this study was to improve salivary gland cell differentiation in primary cultures by using a combination of L1 and a feeder layer of human hair follicle-derived mesenchymal stem cells (hHF-MSCs). Our results indicated that the direct contact of mouse submandibular (mSMG) cell clusters and hHF-MSCs was not required for mSMG cells to form acinar and ductal structures. However, the hHF-MSC conditioned medium enhanced cell organization and multilumen formation, indicating that soluble signals secreted by hHF-MSCs play a role in promoting these features.
School of Dentistry, University of Utah, Salt Lake City, UT, USA.
C L M
CL
Maruyama
School of Dentistry, University of Utah, Salt Lake City, UT, USA.
N J
NJ
Leigh
School of Dentistry, University of Utah, Salt Lake City, UT, USA.
J W
JW
Nelson
Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA.
A D
AD
McCall
School of Dentistry, University of Utah, Salt Lake City, UT, USA.
R E
RE
Mellas
Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA.
P
P
Lei
Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA Department of Biomedical Engineering, School of Engineering and Applied Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA Center of Bioinformatics and Life Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA.
S T
ST
Andreadis
School of Dentistry, University of Utah, Salt Lake City, UT, USA olga.baker@hsc.utah.edu.
O J
OJ
Baker
D000818:Animals; D051402:Aquaporin 5 / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D018859:Hair Follicle / Q000166:cytology; D006801:Humans; D007797:Laminin / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008810:Mice, Inbred C57BL; D018987:Salivary Ducts / Q000166:cytology / Q000254:growth & development; D012469:Salivary Glands / Q000166:cytology* / Q000254:growth & development; D013363:Submandibular Gland / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods
D051402:Aquaporin 5; D007797:Laminin; C121346:laminin 1
acinar cells; aquaporin 5; cyclic AMP; laminin; mesenchymal stromal cell; salivary glands