An efficient in vitro shoot regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.- A dye yielding plant.
Bixa orellana L. (Bixaceae) is a multipurpose tree grown for the production of commercially important dyes. In the present study, an efficient, reproducible protocol was developed for direct plant regeneration from in vitro derived petiole explants of Bixa orellana L. Murashige and Skoog medium (MS) supplemented with 2-isopentenyl adenine (9.8 μM) and naphthalene acetic acid (10.7 μM) was found to be optimum for production of high frequency of shoot organogenesis. Subculturing of the shoots onto the fresh MS medium containing similar concentrations of 2-iP (9.8 μM) and NAA (10.7 μM) produced elongated shoots. Elongated shoots when placed onto MS medium supplemented with 1.7 μM indole-3-acetic acid and 14.7 μM 2-iP produced optimal rooting. Rooted plantlets were acclimatized and transplanted to the field successfully. Histological investigation revealed the origin of shoot primordia, from sub-epidermal cells of petiole explants. The regeneration protocol developed in this study can be useful for mass in vitro propagation and effective genetic transformation of commercially important edible dye yielding tree species.
Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600 Jeli Kelantan, Malaysia ; Department of Botany, Division of Plant Tissue Culture, Sri Venkateswara University, Tirupati, Andhra Pradesh India.
Arifullah
A
Mohammed
Department of Botany, Division of Plant Tissue Culture, Sri Venkateswara University, Tirupati, Andhra Pradesh India.
Kishore K
KK
Chiruvella
Department of Molecular Biology and Cell Biology, Tezpur University, Assam, India.
Nima D
ND
Namsa
Department of Botany, Division of Plant Tissue Culture, Sri Venkateswara University, Tirupati, Andhra Pradesh India.
Rama Gopal
RG
Ghanta
Achiote; Adventitious roots; Histology, rooting, petiole; Lipstick tree