Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays.
Resonant glassy nanostrings have been employed for the detection of biomolecules. These devices offer high sensitivity and amenability to large array integration and multiplexed assays. Such a concept has however been impaired by the lack of stable and biocompatible linker chemistries. Diazonium salt reduction-induced aryl grafting is an aqueous-based process providing strong chemical adhesion. In this work, diazonium-based linker chemistry was performed for the first time on glassy nanostrings, which enabled the bio-functionalization of such devices. Large arrays of nanostrings with ultra-narrow widths down to 10 nm were fabricated employing electron beam lithography. Diazonium modification was first developed on SiCN surfaces and validated by X-ray photoelectron spectroscopy. Similarly modified nanostrings were then covalently functionalized with anti-rabbit IgG as a molecular probe. Specific enumeration of rabbit IgG was successfully performed through observation of downshifts of resonant frequencies. The specificity of this enumeration was confirmed through proper negative control experiments. Helium ion microscopy further verified the successful functionalization of nanostrings.
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. wzheng2@ualberta.ca.
Wei
W
Zheng
Department of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, AB T6G 2G2, Canada. rdu@ualberta.ca.
Rongbing
R
Du
Department of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, AB T6G 2G2, Canada. yong2@ualberta.ca.
Yong
Y
Cao
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. mam20@ualberta.ca.
Mohammad A
MA
Mohammad
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. sdew@ualberta.ca.
Steven K
SK
Dew
Department of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, AB T6G 2G2, Canada. mark.mcdermott@ualberta.ca.
Mark T
MT
McDermott
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. sevoy@ualberta.ca.
Stephane
S
Evoy
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D003979:Diazonium Compounds / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006371:Helium; D007074:Immunoglobulin G / Q000737:chemistry; D007477:Ions; D015335:Molecular Probes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D011817:Rabbits; D017655:Silicon Compounds / Q000737:chemistry; D013499:Surface Properties
D003979:Diazonium Compounds; D007074:Immunoglobulin G; D007477:Ions; D015335:Molecular Probes; D017655:Silicon Compounds; D006371:Helium; C032734:silicon nitride
biosensors; interferometry; nanomechanics; nanostrings