Nucleotide sequence and genome organization of a new proposed crinivirus, tetterwort vein chlorosis virus.
The genome of tetterwort vein chlorosis virus (TVCV) from South Korea has been completely sequenced. Its genomic organization resembles those of other criniviruses, with several new features, indicating that TVCV is a member of a new species in the genus Crinivirus, family Closteroviridae. RNA1 contains 8467 nucleotides, with at least four opening reading frames (ORFs). ORF1a encodes a protein with predicted papain-like protease, methyltransferase, and helicase activities. ORF1b encodes a putative RNA-dependent RNA polymerase that is apparently expressed through a +1 ribosomal frameshift. RNA2 contains 8113 nucleotides encoding at least nine proteins, similar to most crinivirus RNA2s. The 3' untranslated regions of the bipartite RNA genome share 82.1% nucleotide sequence identity.
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, South Korea.|Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
Fumei
F
Zhao
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, South Korea.|Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
Ran Hee
RH
Yoo
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, South Korea.|Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
Seungmo
S
Lim
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, South Korea.|Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea.
Davaajargal
D
Igori
School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, South Korea. suheon@knu.ac.kr.
Su-Heon
SH
Lee
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, South Korea. jsmoon@kribb.re.kr.|Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, South Korea. jsmoon@kribb.re.kr.
Jae Sun
http://orcid.org/0000-0003-0934-1533
JS
Moon
D031174:Atractylodes / Q000821:virology*; D001483:Base Sequence; D029383:Crinivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*