Therapeutic applications of the TAT-mediated protein transduction system for complex I deficiency and other mitochondrial diseases.
Among the five enzyme complexes in the oxidative phosphorylation system, NADH-coenzyme Q oxidoreductase (also called complex I) is the largest, most intricate, and least understood. This enzyme complex spans the inner mitochondrial membrane and catalyzes the first step of electron transfer by the oxidation of NADH, and thereby provides two electrons for the reduction of quinone to quinol. Complex I deficiency is associated with many severe mitochondrial diseases, including Leber hereditary optic neuropathy and Leigh syndrome. However, to date, conventional treatments for the majority of genetic mitochondrial diseases are only palliative. Developing a reliable and convenient therapeutic approach is therefore considered to be an urgent need. Targeted proteins fused with the protein transduction domain of human immunodeficiency virus 1 transactivator of transcription (TAT) have been shown to enter cells by crossing plasma membranes while retaining their biological activities. Recent developments show that, in fusion with mitochondrial targeting sequences (MTSs), TAT-MTS-bound cargo can be correctly transported into mitochondria and restore the missing function of the cargo protein in patients' cells. The available evidence suggests that the TAT-mediated protein transduction system holds great promise as a potential therapeutic approach to treat complex I deficiency, as well as other mitochondrial diseases.
Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan.
Bo-Yu
BY
Lin
Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan.|Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan.
Mou-Chieh
MC
Kao
D000818:Animals; D004272:DNA, Mitochondrial / Q000378:metabolism* / Q000627:therapeutic use; D004274:DNA, Recombinant / Q000378:metabolism / Q000627:therapeutic use; D042967:Electron Transport Complex I / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D056947:Enzyme Replacement Therapy / Q000379:methods; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D028361:Mitochondrial Diseases / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D010085:Oxidative Phosphorylation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D021382:Protein Sorting Signals; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D015398:Signal Transduction; D052416:Targeted Gene Repair / Q000379:methods; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D010446:Peptide Fragments; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D042967:Electron Transport Complex I
complex I; enzyme replacement therapy; mitochondria; oxidative phosphorylation; protein transduction domain; transactivator of transcription peptide