Nano-hydroxyapatite-coated metal-ceramic composite of iron-tricalcium phosphate: Improving the surface wettability, adhesion and proliferation of mesenchymal stem cells in vitro.
Thin radio-frequency magnetron sputter deposited nano-hydroxyapatite (HA) films were prepared on the surface of a Fe-tricalcium phosphate (Fe-TCP) bioceramic composite, which was obtained using a conventional powder injection moulding technique. The obtained nano-hydroxyapatite coated Fe-TCP biocomposites (nano-HA-Fe-TCP) were studied with respect to their chemical and phase composition, surface morphology, water contact angle, surface free energy and hysteresis. The deposition process resulted in a homogeneous, single-phase HA coating. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells (hMSCs) was studied using biological short-term tests in vitro. The surface of the uncoated Fe-TCP bioceramic composite showed an initial cell attachment after 24h of seeding, but adhesion, proliferation and growth did not persist during 14 days of culture. However, the HA-Fe-TCP surfaces allowed cell adhesion, and proliferation during 14 days. The deposition of the nano-HA films on the Fe-TCP surface resulted in higher surface energy, improved hydrophilicity and biocompatibility compared with the surface of the uncoated Fe-TCP. Furthermore, it is suggested that an increase in the polar component of the surface energy was responsible for the enhanced cell adhesion and proliferation in the case of the nano-HA-Fe-TCP biocomposites.
Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
Maria A
MA
Surmeneva
Department of Orthopaedics, Medical University Graz, 8036 Graz, Austria; Institute for Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobel street 12, 70569 Stuttgart, Germany.
Claudia
C
Kleinhans
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany.
Gabriele
G
Vacun
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany; Process Analysis & Technology (PA&T), Reutlingen-University, 72762 Reutlingen, Germany.
Petra Juliane
PJ
Kluger
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany.
Veronika
V
Schönhaar
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany.
Michaela
M
Müller
Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, 28359 Bremen, Germany.
Sebastian Boris
SB
Hein
Technical Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45141 Essen, Germany.
Alexandra
A
Wittmar
Technical Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45141 Essen, Germany.
Mathias
M
Ulbricht
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45117 Essen, Germany.
Oleg
O
Prymak
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany.
Christian
C
Oehr
Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia; Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany. Electronic address: rsurmenev@gmail.com.
Roman A
RA
Surmenev
D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D007501:Iron / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
D002130:Calcium Phosphates; D017886:Durapatite; D007501:Iron; D000469:Alkaline Phosphatase; C018392:tricalcium phosphate
Bioceramic composite; Bioresorbable alloy; Cell adhesion; Hydroxyapatite coating; RF magnetron sputtering