Effect of PDGF-Rb antagonist imatinib on endometrial injury repairing in mouse model.
OBJECTIVE
To study the effects of PDGF-Rb antagonists imatinib on endometrial injury repairing in the mouse model.


METHODS
The cultured MSCs cells from male mice were marked with BrdU in vitro, and then transplanted to the female mice which suffered from radiation injury through tail vein, PDGF-Rb antagonists imatinib was injected through abdominal cavity. Four groups were arranged, which were radiation transplantation group, normal control group, imatinib intervention group and radiation control group. BrdU incorporation, SRY expression and MVD status were detected in uterus of mice.


RESULTS
SRY gene was negative expressed in normal control group and radiation control group. SRY gene presented positive in radiation transplantation group and imatinib intervention group; BrdU incorporation showed negative in radiation control group and normal control group which died in the early stage in mice; the incorporation of BrdU was higher in radiation transplantation group compared with imatinib intervention group; CD34 was positive on the uterus of all the four groups, which showed highest in radiation control group and lowest in radiation control group; The MVD in imatinib intervention group was lower than radiation control group; the difference of MVD was significantly compared with normal control group (P < 0.05).


CONCLUSIONS
PDGF-Rb antagonists imatinib could inhibit the repairing function of MSCs in the endometrial lesions in mice.
Department of Reproductive Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Xin-Rong
XR
Wang
Department of Thoracic Surgery, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Cheng-De
CD
Wang
Department of Reproductive Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Xue-Mei
XM
Liu
Department of Reproductive Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Hong-Chu
HC
Bao
Department of Reproductive Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Qing-Lan
QL
Qu
Department of Reproductive Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China. Electronic address: cuifanghao@aliyun.com.
Cui-Fang
CF
Hao
BrdU; Endometrium; Imatinib mesylate; MVD; PDGF-Rb