The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis.
Angiogenesis, the growth of new blood vessels from preexisting ones, is a fundamental process for organ development, exercise-induced muscle growth, and wound healing, but is also associated with different diseases such as cancer and neovascular eye disease. Accordingly, elucidating the molecular and cellular mechanisms of angiogenesis has the potential to identify new therapeutic targets to stimulate new vessel formation in ischemic tissues or inhibit pathological vessel growth in disease. This chapter describes the mouse embryo hindbrain and postnatal retina as models to study physiological angiogenesis and provides detailed protocols for tissue dissection, sample staining, and analysis.
UCL Institute of Ophthalmology, University College London, 11-43 Bath Street, London, EC1V 9EL, UK, a.fantin@ucl.ac.uk.
Alessandro
A
Fantin
Christiana
C
Ruhrberg
D000818:Animals; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D012171:Retinal Vessels; D012249:Rhombencephalon / Q000098:blood supply* / Q000196:embryology