miR-211 promotes non-small cell lung cancer proliferation by targeting SRCIN1.
MicroRNAs (miRNAs) are a class of small non-coding RNAs that, when dysregulated, are involved in the initiation and progression of various cancers, including lung cancer, in humans. In the current study, qRT-PCR was performed to measure miR-211 expression in human non-small cell lung cancer (NSCLC) cell lines and tissues. Cell proliferation, cell cycle, colony formation, and invasion were performed to detect the functional role of miR-211 in human NSCLC cell line. We used luciferase reporter assay to find the potential target of miR-211. We found that miR-211 expression was upregulated in human non-small cell lung cancer (NSCLC) cell lines and tissues. The overexpression of miR-211 enhanced NSCLC cell proliferation, colony formation, and invasion. SRC kinase signaling inhibitor 1 (SRCIN1) was identified as a direct target of miR-211. SRCIN1 silencing promoted cell proliferation, and SRCIN1 expression was downregulated in human NSCLC cell lines. Thus, miR-211 may function as an oncogenic miRNA in NSCLC, partly by regulating SRCIN1, and the modulation of miR-211 expression represents a potential strategy for the treatment of NSCLC patients.
Department of Pulmonary Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China, 150040.
Leiguang
L
Ye
Department of Pulmonary Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China, 150040.
Hui
H
Wang
Department of Pulmonary Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China, 150040. liubaogang1962@163.com.
Baogang
B
Liu
D000072283:A549 Cells; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness
D033942:Adaptor Proteins, Vesicular Transport; C549405:MIRN211 microRNA, human; D035683:MicroRNAs; C532615:SRCIN1 protein, human
MiR-211; MicroRNA; Non-small cell lung cancer; SRCIN1