Protease Activity and Cell-Free DNA in Blood Plasma of Healthy Donors and Breast Cancer Patients.
Tumor development is generally accompanied by increased protease activity and cell-free DNA (cfDNA) levels in the blood. An immunoassay for protease activity was developed based on the binding of anti-peptide antibodies onto polystyrene plates, followed by incubation with peptides and protein hydrolyzing enzymes. The data obtained demonstrate the peptide CD34-1 composed of uncharged amino acids was the best substrate for the estimation of plasma protease activity in breast cancer patients and healthy donors. Anti-CD34-1 peptide protease activity was shown to correlate with circulating DNA concentrations in cancer patients and healthy subjects (P = 0.001, r = 0.676), demonstrating the role of protease activity in the regulation of cfDNA levels.
a Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental Medicine , Siberian Branch of the Russian Academy of Sciences , Novosibirsk , Russia.|b Faculty of Natural Sciences, Novosibirsk State University , Novosibirsk , Russia.
Svetlana
S
Tamkovich
a Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental Medicine , Siberian Branch of the Russian Academy of Sciences , Novosibirsk , Russia.
Olga
O
Bryzgunova
D001782:Blood Donors; D001943:Breast Neoplasms / Q000097:blood*; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000097:blood*; D010447:Peptide Hydrolases / Q000097:blood*
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D010447:Peptide Hydrolases
ELISA; anti-peptide antibodies; blood plasma; cell-free DNA; immunoassay for protease activity