Increased oxidative stress and apoptosis in peripheral blood mononuclear cells of fructose-fed rats.
BACKGROUND
Measuring of oxidative stress in peripheral blood mononuclear cells is a suitable model of dietary induced systemic oxidative stress. Thus, we aimed to evaluate whether a chronic high fructose intake could induce oxidative damage in peripheral blood and bone marrow mononuclear cells of rats.


METHODS
Animals were randomly assigned to the following groups: Control group (standard rat chow and tap water n=8), and Fructose group (standard rat chow and a 10% fructose solution in the drinking water n=8). Reactive oxygen species and cytokines were measure using flow cytometry in peripheral blood and bone-marrow mononuclear cells. Apoptotic cell death and the advanced oxidation protein products (AOPP) were also determined.


RESULTS
We observed a significant increase in ROS production in peripheral blood mononuclear cells of fructose group as compared to control rats. Apoptosis and the AOPP were higher in those animals underwent high fructose intake. Serum levels of IL-6 and IL-12 were also increased after 12 weeks of high fructose intake.


CONCLUSIONS
We concluded that fructose intake leads to systemic oxidative stress and pro-inflammatory condition which affect peripheral blood mononuclear cells and bone-marrow mononuclear cells viability.
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil.
Marcella L
ML
Porto
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil.
Layla M
LM
Lírio
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil.
Ananda T
AT
Dias
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil.
Alan T
AT
Batista
Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.
Bianca P
BP
Campagnaro
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil.
José G
JG
Mill
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil. Electronic address: meyrelle.vix@terra.com.br.
Silvana S
SS
Meyrelles
Department of Physiological Sciences, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil. Electronic address: marcelobaldo@ymail.com.
Marcelo P
MP
Baldo
D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004032:Diet; D005632:Fructose / Q000008:administration & dosage / Q000633:toxicity*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D062107:Advanced Oxidation Protein Products; D016207:Cytokines; D017382:Reactive Oxygen Species; D005632:Fructose
Apoptosis; Fructose; Inflammation; Mononuclear cells; Oxidative stress