Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors.
Two commercial PRRSV ELISA kits (IDEXX and Bionote) were evaluated for their sensitivity and specificity using 476 PRRS-positive serum samples collected from 7 animal challenge experiments and 1,000 PRRS-negative sera. Both ELISA kits exhibited 100% sensitivity with sera collected 14 to 42 days post-infection, and the results from the kits were highly correlated (R(2)=0.9207). The specificity of IDEXX or Bionote kit was 99.9% or 99.7%, respectively. In addition, the Bionote ELISA kit was used to examine 100 sera that were determined to be falsely positive either by IDEXX 2XR or 3XR ELISA, and only 7 of these samples were found to be positive. These results indicate that both ELISA kits exhibited similar levels of sensitivity and specificity and would complement one another for the verification of false-positive samples.
College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan, Korea.
Byoung-Joo
BJ
Seo
Hyunil
H
Kim
Ho-Seong
HS
Cho
Byoung-Yong
BY
Park
Won-Il
WI
Kim
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine / Q000276:immunology / Q000821:virology
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic