Si Shen Wan Regulates Phospholipase Cγ-1 and PI3K/Akt Signal in Colonic Mucosa from Rats with Colitis.
The present study explored the feasible pathway of Si Shen Wan (SSW) in inhibiting apoptosis of intestinal epithelial cells (IECs) by observing activation of phospholipase Cγ-1 (PLC-γ1) and PI3K/Akt signal in colonic mucosa from rats with colitis. Experimental colitis was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in the Sprague-Dawley rats. After SSW was administrated for 7 days after TNBS infusion, western blot showed an increment in levels of PI3K, p-Akt, and IL-23 and a decrement in levels of PLC-γ1 and HSP70 in colonic mucosal injury induced by TNBS. Meanwhile, assessments by ELISA revealed an increment in concentrations of IL-2, IL-6, and IL-17 and a reduction in level of TGF-β after TNBS challenge. Impressively, treatment with SSW for 7 days significantly attenuated the expressions of PI3K and p-Akt and the secretion of IL-2, IL-6, IL-17, and IL-23 and promoted the activation of PLC-γ1, HSP70, and TGF-β. Our previous studies had demonstrated that SSW restored colonic mucosal ulcers by inhibiting apoptosis of IECs. The present study demonstrated that the effect of SSW on inhibiting apoptosis of IECs was realized probably by activation of PLC-γ1 and suppression of PI3K/Akt signal pathway.
Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330025, China.
Duan-Yong
DY
Liu
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Rong
R
Xu
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Min-Fang
MF
Huang
Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330025, China.
Hong-Yan
HY
Huang
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Xin
X
Wang
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Yong
Y
Zou
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Hai-Yang
HY
Yue
School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Hai-Mei
HM
Zhao