Giant panda (Ailuropoda melanoleuca) sperm morphometry and function after repeated freezing and thawing.
This work examines the effects of subsequent cycles of freezing-thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing-thawing cycles this sperm can withstand. A sperm sample was collected by electroejaculation from a mature giant panda and subjected to five freezing-thawing cycles. Although repeated freezing-thawing negatively affected (P < 0.05) sperm motility and membrane integrity, in both nonselected and DCG-selected sperm samples, >60% of the sperm cells in both treatments showed acrosome integrity even after the fifth freezing cycle. In fresh semen, the sperm head length was 4.7 μm, the head width 3.6 μm, area 14.3 μm(2) and perimeter length 14.1 μm. The present results suggest that giant panda sperm trends to be resistant to repeated freezing-thawing, even without DGC selection.
Departamento de Reproducción Animal, INIA, Madrid, Spain.
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Santiago-Moreno
Departamento de Reproducción Animal, INIA, Madrid, Spain.
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MC
Esteso
Departamento de Reproducción Animal, INIA, Madrid, Spain.|Conselho Nacional de Desenvolvimento Cientifico e Tecnológico - Cnpq, Brasília, Brasil.
J
J
Pradiee
Departamento de Reproducción Animal, INIA, Madrid, Spain.
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C
Castaño
Departamento de Reproducción Animal, INIA, Madrid, Spain.
A
A
Toledano-Díaz
Departamento de Reproducción Animal, INIA, Madrid, Spain.
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E
O'Brien
Departamento de Reproducción Animal, INIA, Madrid, Spain.
A
A
Lopez-Sebastián
Zoo-Aquarium, Madrid, Spain.
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E
Martínez-Nevado
Zoo-Aquarium, Madrid, Spain.
M
M
Delclaux
Zoo-Aquarium, Madrid, Spain.
J
J
Fernández-Morán
Chengdu Research Base of Giant Panda Breeding, Chengdu, China.
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Z
Zhihe
D000177:Acrosome / Q000502:physiology; D000818:Animals; D002499:Centrifugation, Density Gradient; D015925:Cryopreservation / Q000379:methods*; D005615:Freezing; D008297:Male; D008856:Microscopy, Fluorescence; D012661:Semen / Q000000981:diagnostic imaging; D012662:Semen Preservation / Q000379:methods*; D013077:Sperm Head / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D001503:Ursidae / Q000502:physiology*
Cryopreservation; density-gradient centrifugation; giant panda; sperm head morphometry