Upregulation of Collagen Expression via PPARβ/δ Activation in Aged Skin by Magnesium Lithospermate B from Salvia miltiorrhiza.
This study investigated the agonistic activity of magnesium lithospermate B (1), isolated from Salvia miltiorrhiza, on peroxisome proliferator-activated receptor (PPARβ/δ) and the expressions of collagen genes (COL1A1 and COL3A1) and transforming growth factor-β1 (TGF-β1) in models of skin aging. The action of compound 1 as a PPARβ/δ agonist was determined by reporter gene assay, immunostaining, and Western blotting. To determine the antiaging effects of compound 1 on skin, aged Sprague-Dawley rat skin and ultraviolet B (UVB)-irradiated human skin fibroblasts were used. The results show that 1 presented a marked enhancement of both nuclear protein levels and activity of PPARβ/δ in fibroblasts. In addition, 1 prevented downregulation of PPARβ/δ activity in aged rat skin and UVB-induced fibroblasts. Furthermore, 1 increased the expressions of COL1A1, COL3A1, and TGF-β1 in vivo and in a cell culture system. Therefore, the present study shows that compound 1 prevents collagen degradation in aged rat skin and UVB-exposed fibroblasts through PPARβ/δ activation. The therapeutic and cosmetic applications of compound 1 need further investigation.
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Yu Ri
YR
Jung
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Eun Kyeong
EK
Lee
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Dae Hyun
DH
Kim
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.|College of Korean Medicine, Daegu Haany University , Gyeongsan 712-715, Republic of Korea.
Chan Hum
CH
Park
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Min Hi
MH
Park
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Hyoung Oh
HO
Jeong
Graduate School of Science and Engineering for Research, University of Toyama , Toyama 930-8555, Japan.
Takako
T
Yokozawa
Faculty of Pharmaceutical Sciences, Nagasaki University , Nagasaki 852-8131, Japan.
Takashi
T
Tanaka
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Dong Soon
DS
Im
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Nam Deuk
ND
Kim
Department of Physiology, The University of Texas Health Science Center at San Antonio , San Antonio, Texas 78229-3900, United States.
Byung Pal
BP
Yu
Bio-FD&C , Incheon 406-840, Republic of Korea.
Sang Hyun
SH
Mo
Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University , Busan 609-735, Republic of Korea.
Hae Young
HY
Chung
D000368:Aged; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008274:Magnesium / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D047494:PPAR delta / Q000378:metabolism*; D048068:PPAR-beta / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012867:Skin / Q000187:drug effects*; D015533:Transcriptional Activation; D015854:Up-Regulation
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D047494:PPAR delta; D048068:PPAR-beta; C061091:lithospermate B; D003094:Collagen; D008274:Magnesium