Characterization and inhibition studies of carbonic anhydrase from gill of Russian Sturgeon Fish (Acipenser gueldenstaedtii).
An α-carbonic anhydrase (CA, EC 4.2.1.1) was purified and characterized kinetically from gill of Acipenser gueldenstaedtii as an endangered sturgeon species. The carbonic anhydrase was purified 66-folds with yield 20.7% by Sepharose-4B-l-tyrosine-sulfanilamide affinity column and the specific activity was determined as 222.2 EU/mg protein. Km and Vmax kinetic values for gill carbonic anhydrase were calculated by a Lineweaver-Burk graph using p-nitrophenol acetate (p-NPA) as a substrate, and was defined as 2.5 mM and 5 × 10(6 )μM/min, respectively. It was observed that CA from the sturgeon gill in the presence of the sulfanilamide and acetazolamide as an inhibitor had very low IC50 values such as 13.0 and 0.1 μM, respectively. In addition, it was determined that the enzyme was inhibited by Fe(2+,) Co(2+,) Ni(2+), and Zn(2+)-Ba(2+) with the IC50 values of 0.2, 1.7, 1.2, and 1.1 mM, respectively.
a Department of Chemistry , Recep Tayyip Erdoğan University, Faculty of Arts and Sciences , Rize , Turkey and.
Barbaros
B
Dinçer
a Department of Chemistry , Recep Tayyip Erdoğan University, Faculty of Arts and Sciences , Rize , Turkey and.
Arife Pınar
AP
Ekinci
a Department of Chemistry , Recep Tayyip Erdoğan University, Faculty of Arts and Sciences , Rize , Turkey and.
Gülay
G
Akyüz
b Department of Aquaculture , Recep Tayyip Erdoğan University, Fisheries Faculty , Rize , Turkey.
İlker Zeki
İZ
Kurtoğlu
D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005880:Gills / Q000201:enzymology*
D002256:Carbonic Anhydrases
Acipenser gueldenstaedtii; Russian Sturgeon; carbonic anhydrase; gill