Cloning and Expression of Plantaricin W Produced by Lactobacillus plantarum U10 Isolate from "Tempoyak" Indonesian Fermented Food as Immunity Protein in Lactococcus lactis.
Plantaricins, one of bacteriocin produced by Lactobacillus plantarum, are already known to have activities against several pathogenic bacterium. L. plantarum U10 isolated from "tempoyak," an Indonesian fermented food, produced one kind of plantaricin designated as plantaricin W (plnW). The plnW is suggested as a putative membrane location of protein and has similar conserved motif which is important as immunity to bacteriocin itself. Thus, due to study about this plantaricin, several constructs have been cloned and protein was analyzed in Lactococcus lactis. In this study, plnW gene was successfully cloned into vector NICE system pNZ8148 and created the transformant named L. lactis NZ3900 pNZ8148-WU10. PlnW protein was 25.3 kDa in size. The concentration of expressed protein was significantly increased by 10 ng/mL nisin induction. Furthermore, PlnW exhibited protease activity with value of 2.22 ± 0.05 U/mL and specific activity about 1.65 ± 0.03 U/mg protein with 50 ng/mL nisin induction. Immunity study showed that the PlnW had immunity activity especially against plantaricin and rendered L. lactis recombinant an immunity broadly to other bacteriocins such as pediocin, fermentcin, and acidocin.
Research Center for Biotechnology, Indonesian Institute of Science (LIPI), Cibinong, Bogor, 16911, West Java, Indonesia.|School of Biotechnology, Bogor Agricultural University, Darmaga, Bogor, 16680, West Java, Indonesia.
Aksar Chair
AC
Lages
Research Center for Biotechnology, Indonesian Institute of Science (LIPI), Cibinong, Bogor, 16911, West Java, Indonesia. azmustopa@yahoo.com.
Apon Zaenal
AZ
Mustopa
Research Center for Biotechnology, Indonesian Institute of Science (LIPI), Cibinong, Bogor, 16911, West Java, Indonesia.
Linda
L
Sukmarini
School of Biotechnology, Bogor Agricultural University, Darmaga, Bogor, 16680, West Java, Indonesia.|Research Center of Bioresources and Biotechnology, Bogor Agricultural University, Darmaga, Bogor, 16680, West Java, Indonesia.
Suharsono
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D005285:Fermentation; D005511:Food Handling; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data
D001430:Bacteriocins
Immunity; L. lactis; Plantaricin; PlnW; pNZ8148