Quantification of mequitazine in human plasma by gas chromatography- quadrupole mass spectrometry and its application to a human pharmacokinetic study.
A specific and sensitive gas chromatography-mass spectrometry (GC-MS) with quadrupole mass analyzer type was developed and validated for the quantitative analysis of mequitazine in human plasma. After liquid-liquid extraction of plasma samples containing mequitazine and promethazine (internal standard, IS) using hexane with pH adjustment, the extract was evaporated and an aliquot of reconstituted residue was injected into the GC-MS system. The assay showed linearity over a concentration range from 1 to 50 ng/mL. Intra- and inter-day precision for mequitazine was <9.09 and 9.29%, respectively, and intra- and inter-day accuracy ranged from -7.97 to 9.05% and from -1.51 to 7.89%, respectively. The lower limit of quantification was 1 ng/mL in the present assay. The developed analytical method was successfully applied to a pharmacokinetic study after a single oral administration of mequitazine in human subjects.
College of Pharmacy, Kangwon National University, Chuncheon, 200-701, Republic of Korea.|Research Institute of Pharmaceutical Sciences, Seoul National University, San 56-1, Shinlim-dong, Kwanak-gu, Seoul, 151-742, Republic of Korea.
Sung-Hoon
SH
Ahn
College of Pharmacy, Gachon University, 191 Hambakmoei-ro, Yeonsu-gu, Incheon, 406-799, Republic of Korea.
Han-Joo
HJ
Maeng
D000284:Administration, Oral; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D010640:Phenothiazines / Q000008:administration & dosage / Q000097:blood*; D015203:Reproducibility of Results
D006634:Histamine H1 Antagonists; D010640:Phenothiazines; C006069:mequitazine
gas chromatography-mass spectrometry (GC-MS); human plasma; mequitazine; pharmacokinetics; quadrupole