Association between TRAIL gene polymorphisms and the susceptibility and severity of lumbar disc degeneration.
OBJECTIVE
The aim of the present study was to investigate the association between tumor necrosis factor related apoptosis-inducing ligand (TRAIL) gene polymorphisms and the susceptibility and severity of lumbar disc degeneration (LDD) in the Chinese Han population.


METHODS
A total of 153 patients with LDD and 131 healthy subjects were enrolled in the study. Four single-nucleotide polymorphisms (SNPs) in the 3' untranslated region (3'UTR) of TRAIL gene, including 1289 C/A, 1525 G/A, 1588 G/A and 1595 C/T, were genotyped with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.


RESULTS
The genotypes and alleles frequencies of TRAIL at 1525 and 1595 positions in all subjects were the same. There was a significant association between TRAIL 1525/1595 polymorphisms and the susceptibility of LDD. The frequencies of 1525 GG /1595 CC genotype, and 1525 G/1595 C allele were higher in the patients group than that in the control group. In addition, we found patients with the 1525 AA /1595 TT genotype, as well as 1525 A/1595 T allele exhibit significantly low frequency of high grades of disc degeneration. However, there were no significant differences in the genotype or allele distribution of TRAIL 1289 C/A or 1588 G/A between the patients and the control group.


CONCLUSIONS
TRAIL 1525/1595 polymorphisms were associated with the susceptibility and severity of LDD in the Chinese Han population.
Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi, China.
Heng
H
Du
Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi, China.
Bin
B
Bai
Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi, China.
Yusheng
Y
Qiu
Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi, China.
Si
S
Yin
Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi, China.
Weiguo
W
Bian
D000328:Adult; D044466:Asian People / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000235:genetics*; D008159:Lumbar Vertebrae; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics*; D055815:Young Adult
D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human
Tumor necrosis factor related apoptosis-inducing ligand; lumbar disc degeneration; polymorphism; severity; susceptibility