Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid.
An in vitro approach to the production of rosmarinic acid (RA), a medicinally important caffeic acid ester, in a cell suspension culture (CSC) of Satureja khuzistanica Jamzad (Lamiaceae) has been investigated for the first time. The CSC was established from friable calli derived from shoot tip explants in Gamborg's B5 liquid medium supplemented with 30 g/L sucrose, 20 mg/L L-glutamine, 200 mg/L casein hydrolysate, 5 mg/L benzyladenine (BA) and 1 mg/L indole-3-butyric acid (IBA). The effect of nitrogen source (KNO3 and (NH4)2SO4) and their different concentrations on the fresh and dry weight (g/L), as well as RA content (mg/g dry weight) were measured. CSC growth measurements indicated a maximum specific cell growth rate of 1.5/day, a doubling time of 7.6 days and a high percentage of cell viability (96.4 %) throughout the growth cycle. Maximum cell fresh weight (353.5 g/L), dry weight (19.7 g/L) and RA production (180.0 mg/g) were attained at day 21 of culture. Cell growth and RA content were affected by nitrogen deficiency. Media containing 8.3 mM of total nitrogen (¼ of B5 standard medium) led to a minimum cell fresh weight (243.0 g/L), dry weight (17.4 g/L) and RA content (38.0 mg/g) after 21 days. The established CSC provided useful material for further optimization experiments aimed at a large-scale production of RA.
Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran.|Department of Horticulture, Faculty of Agriculture, University of Guilan, Rasht, Iran.
Amir
A
Sahraroo
Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University G. C., Tehran, Iran. m-mirjalili@sbu.ac.ir.
Mohammad Hossein
MH
Mirjalili
Department of Plant Physiology, Campus Miguel de Unamuno, University of Salamanca, 37007, Salamanca, Spain.
Purificación
P
Corchete
Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran.
Mesbah
M
Babalar
Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran.
Mohammad Reza
MR
Fattahi Moghadam
Callus; Cell suspension culture; Lamiaceae; Rosmarinic acid; Satureja sp.