A biosensor for the detection of single base mismatches in microRNA.
Graphene oxide quenches fluorescence corresponding to only a mismatched target due to selective denaturing of the thermo-unstable duplex composed of probe peptide nucleic acid and single base mismatched target RNA and thus, the fluorescence signal only from perfectly matched target RNA is measured.
Center for RNA Research, Institute for Basic Science, Seoul National University, Seoul, 151-747, Korea. dalheemin@snu.ac.kr.
Jieon
J
Lee
Ginam
G
Park
Dal-Hee
DH
Min
D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D035683:MicroRNAs / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D035683:MicroRNAs