Mitochondrial Haplotypes Influence Metabolic Traits in Porcine Transmitochondrial Cybrids.
In farm animals, mitochondrial DNA mutations exist widely across breeds and individuals. In order to identify differences among mtDNA haplotypes, two porcine transmitochondrial cybrids were generated by fusion of a Lantang pig cell line devoid of mitochondrial DNA with enucleated cytoplasm from either a Large White pig or a Xiang pig harboring potentially divergent mitochondrial haplotypes. These cybrid cells were subjected to mitochondrial genome sequencing, copy number detecting and analysis of biochemical traits including succinate dehydrogenase (SDH) activity, ATP content and susceptibility to reactive oxygen species (ROS). The Lantang and Xiang mitochondrial genomes were highly homologous with only 18 polymorphic sites, and differed radically from the Large White with 201 and 198 mutations respectively. The Large White and Xiang cybrids exhibited similar mtDNA copy numbers and different values among biochemical traits, generated greater ROS production (P < 0.05) and less SDH activity (P < 0.05) and a lesser ATP content (P < 0.05). The results show that functional differences exist between cybrid cells which differ in mitochondrial genomic background. In conclusion, transmitochondrial cybrids provide the first direct evidence on pig biochemical traits linking different mitochondrial genome haplotypes.
National Engineering Laboratory for Animal Breeding; Ministry of Agricultural Key Laboratory of Animal Genetics, Breeding and Reproduction; College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China.
Guanghui
G
Yu
National Engineering Laboratory for Animal Breeding; Ministry of Agricultural Key Laboratory of Animal Genetics, Breeding and Reproduction; College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China.
Hai
H
Xiang
National Engineering Laboratory for Animal Breeding; Ministry of Agricultural Key Laboratory of Animal Genetics, Breeding and Reproduction; College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China.
Jianhui
J
Tian
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China.
Jingdong
J
Yin
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, and Department of Biological Sciences, College of Arts and Sciences, The University of Alabama, Tuscaloosa, AL 35487, USA.
Carl A
CA
Pinkert
State Key Laboratory for Agribiotechnology; College of Biological Sciences, China Agricultural University, 100193, Beijing, China.
Qiuyan
Q
Li
National Engineering Laboratory for Animal Breeding; Ministry of Agricultural Key Laboratory of Animal Genetics, Breeding and Reproduction; College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China.
Xingbo
X
Zhao
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D054629:Genome, Mitochondrial / Q000235:genetics; D006239:Haplotypes / Q000235:genetics*; D006822:Hybrid Cells / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics*; D020125:Mutation, Missense / Q000235:genetics; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism; D015203:Reproducibility of Results; D013385:Succinate Dehydrogenase / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics*
D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase