Regulation of kisspeptin and gonadotropin-releasing hormone expression in rat placenta: study using primary cultures of rat placental cells.
BACKGROUND
Gonadotropin-releasing hormone (GnRH) and kisspeptin in the hypothalamus are thought to be crucial components of the hypothalamic-pituitary-gonadal (HPG) axis and maintain reproductive function. These neuropeptides are also expressed in the placenta, where they may contribute to placental physiology. In this study, we examined how these peptides are regulated within the placenta.


METHODS
We used primary cultures of placental tissue from rats of 16-18 days gestation. After stimulation with estradiol, GnRH, kisspeptin, and neurokinin B (NKB), changes in placental GnRH, kisspeptin, and human chorionic gonadotropin (hCG) mRNA expression were evaluated by real-time quantitative RT-PCR analysis.


RESULTS
Immunocytochemical analysis showed that rat placental cells contained cells expressing kisspeptin or GnRH. GnRH and kisspeptin mRNA expression was significantly increased in placental cells in the presence of estradiol; NKB mRNA expression was also stimulated by estradiol. Stimulation of the cells with kisspeptin failed to stimulate GnRH mRNA expression. Conversely, both GnRH itself and NKB increased GnRH mRNA expression. Kisspeptin mRNA expression was not increased by kisspeptin itself; however, GnRH and NKB significantly increased kisspeptin mRNA expression. hCG expression was increased in the presence of estradiol. In addition, kisspeptin, GnRH, and NKB could stimulate the expression of hCG mRNA in placental cells.


CONCLUSIONS
Our experiments using primary cultures of rat placental cells showed that GnRH, kisspeptin, and NKB expression was enhanced by estradiol, and unlike in the hypothalamus, kisspeptin did not control the expression of GnRH in placental cells. NKB might be located upstream of kisspeptin and GnRH, and these neuropeptides might be involved in the induction of hCG expression in placental cells.
Department of Obstetrics and Gynecology, Shimane University School of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. oride@med.shimane-u.ac.jp.
Aki
A
Oride
Department of Obstetrics and Gynecology, Shimane University School of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. kanasaki@med.shimane-u.ac.jp.
Haruhiko
H
Kanasaki
Department of Obstetrics and Gynecology, Shimane University School of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. tselmeg@med.shimane-u.ac.jp.
Tselmeg
T
Mijiddorj
Department of Obstetrics and Gynecology, Shimane University School of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. unur@med.shimane-u.ac.jp.
Unurjargal
U
Sukhbaatar
Department of Obstetrics and Gynecology, Shimane University School of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. tishihara@med.shimane-u.ac.jp.
Tomoko
T
Ishihara
Department of Obstetrics and Gynecology, Shimane University School of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan. kyo@med.shimane-u.ac.jp.
Satoru
S
Kyo
D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D059648:Kisspeptins / Q000096:biosynthesis* / Q000235:genetics; D015287:Neurokinin B / Q000096:biosynthesis / Q000235:genetics; D010920:Placenta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats
C491406:Kiss1 protein, rat; D059648:Kisspeptins; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D015287:Neurokinin B