Sulphate production by Paracoccus pantotrophus ATCC 35512 from different sulphur substrates: sodium thiosulphate, sulphite and sulphide.
One of the problems in waste water treatment plants (WWTPs) is the increase in emissions of hydrogen sulphide (H2S), which can cause damage to the health of human populations and ecosystems. To control emissions of this gas, sulphur-oxidizing bacteria can be used to convert H2S to sulphate. In this work, sulphate detection was performed by spectrophotometry, ion chromatography and atomic absorption spectrometry, using Paracoccus pantotrophus ATCC 35512 as a reference strain growing in an inorganic broth supplemented with sodium thiosulphate (Na2S2O3·5H2O), sodium sulphide (Na2S) or sodium sulphite (Na2SO3), separately. The strain was metabolically competent in sulphate production. However, it was only possible to observe significant differences in sulphate production compared to abiotic control when the inorganic medium was supplemented with sodium thiosulphate. The three methods for sulphate detection showed similar patterns, although the chromatographic method was the most sensitive for this study. This strain can be used as a reference for sulphate production in studies with sulphur-oxidizing bacteria originating from environmental samples of WWTPs.
a Departamento de Microbiologia , Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul , Rua Sarmento Leite 500, 90050-170 Porto Alegre , Brazil.
Daniel Derrossi
DD
Meyer
b Escola Superior de Agricultura Luiz de Queiroz (ESALQ) , Universidade de São Paulo , Av. Pádua Dias 11, 13418-900 Piracicaba , Brazil.
Felipe Gabriel
FG
Andrino
b Escola Superior de Agricultura Luiz de Queiroz (ESALQ) , Universidade de São Paulo , Av. Pádua Dias 11, 13418-900 Piracicaba , Brazil.
Simone
S
Possedente de Lira
c Departamento de Ciências Atmosféricas , Instituto de Astronomia, Geofísica e Ciências Atmosféricas, Universidade de São Paulo , Rua do Matão 1226, 05508-090 São Paulo , Brazil.
Adalgiza
A
Fornaro
a Departamento de Microbiologia , Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul , Rua Sarmento Leite 500, 90050-170 Porto Alegre , Brazil.
Gertrudes
G
Corção
d Departamento de Ciência de Alimentos , Universidade Federal do Rio Grande do Sul , Av. Bento Gonçalves 9500, 91501-970 Porto Alegre , Brazil.
Adriano
A
Brandelli
D042084:Paracoccus pantotrophus / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
D013431:Sulfates; D013440:Sulfides; D013447:Sulfites; D013885:Thiosulfates; C017717:sodium thiosulfate; C025026:sodium sulfite; C033479:sodium sulfide
H2S; Paracoccus pantotrophus; Sulphur-oxidizing bacteria; sulphate