Pretreating wheat straw by the concentrated phosphoric acid plus hydrogen peroxide (PHP): Investigations on pretreatment conditions and structure changes.
Wheat straw was pretreated by PHP (the concentrated H3PO4 plus H2O2) to clarify effects of temperature, time and H3PO4 proportion on hemicellulose removal, delignification, cellulose recovery and enzymatic digestibility. Overall, hemicellulose removal was intensified by PHP comparing to the concentrated H3PO4. Moreover, efficient delignification specially happened in PHP pretreatment. Hemicellulose removal and delignification by PHP positively responded to temperature and time. Increasing H3PO4 proportion in PHP can promote hemicellulose removal, however, decrease the delignification. Maximum hemicellulose removal and delignification were achieved at 100% and 83.7% by PHP. Enzymatic digestibility of PHP-pretreated wheat straw was greatly improved by increasing temperature, time and H3PO4 proportion, and complete hydrolysis can be achieved consequently. As temperature of 30-40°C, time of 2.0 h and H3PO4 proportion of 60% were employed, more than 92% cellulose was retained in the pretreated wheat straw, and 29.1-32.6g glucose can be harvested from 100g wheat straw.
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Qing
Q
Wang
Forest Products Biotechnology, Department of Wood Science, The University of British Columbia, Vancouver V6T 1Z4, BC, Canada.
Jinguang
J
Hu
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China. Electronic address: fishensjtu@gmail.com.
Fei
F
Shen
Biogas Institute of Ministry of Agriculture, Chengdu, Sichuan 610041, PR China.
Zili
Z
Mei
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Gang
G
Yang
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Yanzong
Y
Zhang
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Yaodong
Y
Hu
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Jing
J
Zhang
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Shihuai
S
Deng
D002482:Cellulose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D010756:Phosphoric Acids / Q000494:pharmacology*; D011134:Polysaccharides / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014908:Triticum / Q000737:chemistry* / Q000187:drug effects; D014866:Waste Products
D010756:Phosphoric Acids; D011134:Polysaccharides; D014866:Waste Products; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D006861:Hydrogen Peroxide; C030242:phosphoric acid; D005947:Glucose
Delignification; Enzymatic hydrolysis; Hemicellulose removal; Phosphoric acid plus hydrogen peroxide; Structure characteristics