Chondroitin sulfate effects on neural stem cell differentiation.
We have investigated the role chondroitin sulfate has on cell interactions during neural plate formation in the early chick embryo. Using tissue culture isolates from the prospective neural plate, we have measured neural gene expression profiles associated with neural stem cell differentiation. Removal of chondroitin sulfate from stage 4 neural plate tissue leads to altered associations of N-cadherin-positive neural progenitors and causes changes in the normal sequence of neural marker gene expression. Absence of chondroitin sulfate in the neural plate leads to reduced Sox2 expression and is accompanied by an increase in the expression of anterior markers of neural regionalization. Results obtained in this study suggest that the presence of chondroitin sulfate in the anterior chick embryo is instrumental in maintaining cells in the neural precursor state.
Department of Biological Sciences, Murray State University, Murray, KY, 42071, USA. dcanning@murraystate.edu.
David R
DR
Canning
Department of Biological Sciences, Murray State University, Murray, KY, 42071, USA.
Natalie R
NR
Brelsford
Department of Biological Sciences, Murray State University, Murray, KY, 42071, USA.
Neil W
NW
Lovett
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D054258:Neural Plate / Q000187:drug effects / Q000254:growth & development; D058953:Neural Stem Cells / Q000187:drug effects*; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis
D055748:SOXB1 Transcription Factors; D002809:Chondroitin Sulfates
Chick embryo; Chondroitin sulfate; Neural plate