The immune adherence receptor CR1-like existed on porcine erythrocytes membrane.
In the present study, we obtain a mouse anti-porcine complement receptor type 1 (CR1)-like monoclonal antibody (McAb) and use this McAb to verify the existence of CR1-like protein on porcine erythrocytes. Our results confirm that CR1-like protein is localized on the surface of porcine erythrocytes. Mouse immunoglobulin G inhibited the binding of serum-opsonized green fluorescent protein-expressing Escherichia coli to porcine erythrocytes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicates that CR1-like McAb reacts with biochemically-purified porcine erythrocyte membrane fractions, with a clear band at 135 kDa to 140 kDa. We postulate that the 135 kDa to 140 kDa membrane protein is the equivalent of the porcine erythrocyte CR1-like protein.
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Wei
W
Yin
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Jiaoyan
J
Cui
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Junbing
J
Jiang
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Junxing
J
Zhao
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Kuohai
K
Fan
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Na
N
Sun
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Zhiwei
Z
Wang
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Yaogui
Y
Sun
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Haili
H
Ma
College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Shanxi 030801, P. R. China.
Hongquan
H
Li
D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry; D015153:Blotting, Western; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D008807:Mice, Inbred BALB C; D017463:Receptors, Complement 3b / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D034421:Sus scrofa
D058846:Antibodies, Monoclonal, Murine-Derived; D017463:Receptors, Complement 3b