Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections.
We reported previously that baculovirus AcMNPV host-ranges in silkworm strains are controlled by a novel third chromosomal locus. To further isolate the potential host factor and uncover the functional pathway involved, in this study we analyzed hemolymph proteins from AcMNPV-resistant or -sensitive silkworm strains infected with baculoviruses. All the protein spots from 2D electrophoresis were characterized by MALDI-TOF MS and further systematically assessed for differentially regulated proteins at different stages of infection. Subsequently, six candidates were selected for functional analysis using Bm5 cells, where the candidates were knocked-down or overexpressed. We observed that mRNA expression levels of beta-N-acetylglucosaminidase and prophenoloxidase subunit 2 are significantly upregulated during AcMNPV infections in Bm5 cells. Ultimately, we found that RNA interference of ribosomal protein RpL34 causes serious damages to cell viability as well as abortive infection, indicating that ribosomal components are essential for productive baculovirus infection.
Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Jian
J
Xu
Laboratory of Silkworm Genetic Resources, Institute of Genetic Resources, Graduate School of Bio Resources and Bioenvironmental Science, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
Pingbo
P
Zhang
Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Takahiro
T
Kusakabe
Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Hiroaki
H
Mon
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Zhiqing
Z
Li
Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Li
L
Zhu
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
Kazuhiro
K
Iiyama
Laboratory of Silkworm Genetic Resources, Institute of Genetic Resources, Graduate School of Bio Resources and Bioenvironmental Science, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan.
Yutaka
Y
Banno
Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Daisuke
D
Morokuma
Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: jaemanle@agr.kyushu-u.ac.jp.
Jae Man
JM
Lee
D000118:Acetylglucosaminidase / Q000032:analysis / Q000235:genetics; D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000235:genetics / Q000821:virology*; D004156:Catechol Oxidase / Q000032:analysis / Q000235:genetics; D002460:Cell Line; D004792:Enzyme Precursors / Q000032:analysis / Q000235:genetics; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000378:metabolism / Q000821:virology*; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000032:analysis* / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000302:isolation & purification / Q000502:physiology*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004792:Enzyme Precursors; D019476:Insect Proteins; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D000118:Acetylglucosaminidase
Baculovirus; Hemolymph proteins; Proteomic analysis; Silkworm