Performance of the CLSI Carba NP and the Rosco Carb Screen Assays Using North American Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa Isolates.
This study compared the performance of the Carba NP assay, published by the Clinical and Laboratory Standards Institute, and the Rosco Rapid Carb Screen kit. Carba NP had superior sensitivity, but both assays required an increased inoculum to detect carbapenemase production in isolates with blaNDM, blaIMP, and blaOXA-48.
Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Lauren C
LC
Gallagher
Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Sylvester S
SS
Roundtree
Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Diana P
DP
Lancaster
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center and Case Western Reserve School of Medicine, Cleveland, Ohio, USA.
Susan D
SD
Rudin
Department of Pathology and Laboratory Medicine, The Children's Hospital of Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
Jennifer Dien
JD
Bard
Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College at Thomas Jefferson University and Hospitals, Philadelphia, Pennsylvania, USA.
Amity L
AL
Roberts
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center and Case Western Reserve School of Medicine, Cleveland, Ohio, USA.
Steven H
SH
Marshall
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center and Case Western Reserve School of Medicine, Cleveland, Ohio, USA Department of Molecular Biology and Microbiology, Case Western Reserve School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Robert A
RA
Bonomo
Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA otak@email.chop.edu.
Kaede V
KV
Sullivan
D001426:Bacterial Proteins / Q000032:analysis*; D004755:Enterobacteriaceae / Q000201:enzymology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009656:North America; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis*
D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase