Adiponectin in Fresh Frozen Plasma Contributes to Restoration of Vascular Barrier Function After Hemorrhagic Shock.
Hemorrhagic shock is the leading cause of preventable deaths in civilian and military trauma. Use of fresh frozen plasma (FFP) in patients requiring massive transfusion is associated with improved outcomes. FFP contains significant amounts of adiponectin, which is known to have vascular protective function. We hypothesize that FFP improves vascular barrier function largely via adiponectin. Plasma adiponectin levels were measured in 19 severely injured patients in hemorrhagic shock (HS). Compared with normal individuals, plasma adiponectin levels decreased to 49% in HS patients before resuscitation (P < 0.05) and increased to 64% post-resuscitation (but not significant). In a HS mouse model, we demonstrated a similar decrease in plasma adiponectin to 54% but a significant increase to 79% by FFP resuscitation compared with baseline (P < 0.05). HS disrupted lung vascular barrier function, leading to an increase in permeability. FFP resuscitation reversed these HS-induced effects. Immunodepletion of adiponectin from FFP abolished FFP's effects on blocking endothelial hyperpermeability in vitro, and on improving lung vascular barrier function in HS mice. Replenishment with adiponectin rescued FFP's effects. These findings suggest that adiponectin is an important component in FFP resuscitation contributing to the beneficial effects on vascular barrier function after HS.
Department of Surgery, The University of Texas Health Science Center at Houston, TX USA.
Xiyun
X
Deng
Department of Surgery, The University of Texas Health Science Center at Houston, TX USA.
Yanna
Y
Cao
Center for Translational Injury Research, The University of Texas Health Science Center at Houston, TX USA.
Maria P
MP
Huby
Department of Surgery, The University of Texas Health Science Center at Houston, TX USA.|Medical Science Institute, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
Chaojun
C
Duan
Center for Translational Injury Research, The University of Texas Health Science Center at Houston, TX USA.
Lisa
L
Baer
Shock Trauma Center, University of Maryland, Baltimore, MD USA.
Zhanglong
Z
Peng
Shock Trauma Center, University of Maryland, Baltimore, MD USA.
Rosemary A
RA
Kozar
Department of Anesthesiology, The University of Texas Health Science Center at Houston, TX USA.
Marie-Francoise
MF
Doursout
Department of Surgery, The University of Texas Health Science Center at Houston, TX USA.|Center for Translational Injury Research, The University of Texas Health Science Center at Houston, TX USA.
John B
JB
Holcomb
Department of Surgery, The University of Texas Health Science Center at Houston, TX USA.|Center for Translational Injury Research, The University of Texas Health Science Center at Houston, TX USA.
Charles E
CE
Wade
Department of Surgery, The University of Texas Health Science Center at Houston, TX USA.
Tien C
TC
Ko
D052242:Adiponectin / Q000097:blood / Q000502:physiology / Q000627:therapeutic use*; D000328:Adult; D002199:Capillary Permeability / Q000187:drug effects* / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000503:physiopathology; D005260:Female; D006801:Humans; D008168:Lung / Q000098:blood supply; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry*; D012151:Resuscitation / Q000379:methods; D012771:Shock, Hemorrhagic / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D055815:Young Adult
C499918:ADIPOQ protein, human; D052242:Adiponectin