HPTLC and GC/MS Study of Amaryllidaceae Alkaloids of Two Narcissus Species.
In this article, we report on the alkaloid profile and dynamic of alkaloid content and diversity in two Narcissus plants at different stages of development. The alkaloid profile of the two Narcissus species was investigated by GC/MS and HPTLC. Fifty eight Amaryllidaceae alkaloids were detected, and 25 of them were identified in the different organs of N. tazetta and N. papyraceus. The alkaloid 3-O-methyl-9-O-demethylmaritidine is tentatively identified here for the first time from the Amaryllidaceae family, and four alkaloids (tazettamide, sternbergine, 1-O-acetyllycorine, 2,11-didehydro-2-dehydroxylycorine) are tentatively identified for the first time in the genus Narcissus. The different organs of the two species analyzed showed remarkable differences in their alkaloid pattern, type of biosynthesis, main alkaloid and number of alkaloids. Lycorine-type alkaloids dominated the alkaloid, metabolism in N. papyraceus, while alkaloids of narciclasine-, galanthamine- and homolycorine-types were found only in the species N. tazetta L.
Alkhartoom Square, Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt (phone: +201005294669). shawkyeman@yahoo.com.
Eman
E
Shawky
Alkhartoom Square, Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt (phone: +201005294669).
Amina H
AH
Abou-Donia
Alkhartoom Square, Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt (phone: +201005294669).
Fikria A
FA
Darwish
Alkhartoom Square, Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt (phone: +201005294669).
Soad M
SM
Toaima
Alkhartoom Square, Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt (phone: +201005294669).
Sarah S
SS
Takla
Department de Productes Naturals, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, ES-08028-Barcelona.
Natalia B
NB
Pigni
Department de Productes Naturals, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, ES-08028-Barcelona.
Jaume
J
Bastida
D000470:Alkaloids / Q000032:analysis*; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D031427:Narcissus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
D000470:Alkaloids; D010936:Plant Extracts
Alkaloids; Narcissus papyraceus; Narcissus tazetta; Ontogenic variation