Analysis of Arginylated Peptides by Subtractive Edman Degradation.
During the early studies of N-terminal arginylation, Edman degradation was widely used to identify N-terminally added Arg on protein substrates. This old method is reliable but highly depends on the purity and abundance of samples and can become misleading unless a highly purified, highly arginylated protein can be obtained. Here we report a mass spectrometry-based method that utilizes Edman degradation chemistry to identify arginylation in more complex and less abundant protein samples. This method can also apply to the analysis of other posttranslational modifications.
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, 19104, USA.
Anna S
AS
Kashina
John R
JR
Yates
D019881:Aminoacyltransferases / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D059748:Proteolysis
D010455:Peptides; D001120:Arginine; D019881:Aminoacyltransferases