Hybrid mimics and hybrid vigor in Arabidopsis.
F1 hybrids can outperform their parents in yield and vegetative biomass, features of hybrid vigor that form the basis of the hybrid seed industry. The yield advantage of the F1 is lost in the F2 and subsequent generations. In Arabidopsis, from F2 plants that have a F1-like phenotype, we have by recurrent selection produced pure breeding F5/F6 lines, hybrid mimics, in which the characteristics of the F1 hybrid are stabilized. These hybrid mimic lines, like the F1 hybrid, have larger leaves than the parent plant, and the leaves have increased photosynthetic cell numbers, and in some lines, increased size of cells, suggesting an increased supply of photosynthate. A comparison of the differentially expressed genes in the F1 hybrid with those of eight hybrid mimic lines identified metabolic pathways altered in both; these pathways include down-regulation of defense response pathways and altered abiotic response pathways. F6 hybrid mimic lines are mostly homozygous at each locus in the genome and yet retain the large F1-like phenotype. Many alleles in the F6 plants, when they are homozygous, have expression levels different to the level in the parent. We consider this altered expression to be a consequence of transregulation of genes from one parent by genes from the other parent. Transregulation could also arise from epigenetic modifications in the F1. The pure breeding hybrid mimics have been valuable in probing the mechanisms of hybrid vigor and may also prove to be useful hybrid vigor equivalents in agriculture.
Agriculture, Commonwealth Scientific Industrial Research Organisation, Canberra, ACT 2601, Australia;
Li
L
Wang
Agriculture, Commonwealth Scientific Industrial Research Organisation, Canberra, ACT 2601, Australia;
Ian K
IK
Greaves
Agriculture, Commonwealth Scientific Industrial Research Organisation, Canberra, ACT 2601, Australia;
Michael
M
Groszmann
Agriculture, Commonwealth Scientific Industrial Research Organisation, Canberra, ACT 2601, Australia;
Li Min
LM
Wu
Agriculture, Commonwealth Scientific Industrial Research Organisation, Canberra, ACT 2601, Australia; Faculty of Science, University of Technology, Sydney, NSW 2007, Australia.
Elizabeth S
ES
Dennis
Agriculture, Commonwealth Scientific Industrial Research Organisation, Canberra, ACT 2601, Australia; Faculty of Science, University of Technology, Sydney, NSW 2007, Australia jim.peacock@csiro.au.
W James
WJ
Peacock
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D006720:Homozygote; D006823:Hybrid Vigor; D018515:Plant Leaves; D020641:Polymorphism, Single Nucleotide
TCM; epigenetics; heterosis; transcriptome; transregulation