Identification of recombinant human EPO variants in greyhound plasma and urine by ELISA, LC-MS/MS and western blotting: a comparative study.
The recombinant human erythropoietins epoetin alfa (Eprex®), darbepoetin (Aranesp®) and methoxy polyethylene glycol-epoetin beta (Mircera®) were administered to greyhounds for 7, 10 and 14 days respectively. Blood and urine samples were collected and analysed for erythropoietin by ELISA, LC-MS/MS and western blotting. Limits of confirmation in plasma for western blotting and LC-MS/MS methods ranged from a low of 2.5mIU/mL, and closely matched the sensitivity of ELISA screening.
Biological Research Unit, Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
Mark
M
Timms
Biological Research Unit, Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
Rohan
R
Steel
Biological Research Unit, Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
John
J
Vine
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D000068256:Darbepoetin alfa; D004285:Dogs; D004300:Doping in Sports / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D000068817:Epoetin Alfa; D004921:Erythropoietin / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000493:pharmacokinetics; D012015:Reference Standards
D011994:Recombinant Proteins; C103998:epoetin beta; D004921:Erythropoietin; D000068256:Darbepoetin alfa; D000068817:Epoetin Alfa
Aranesp®; ELISA; Eprex®; Mircera®; erythropoietin; greyhounds; western blotting