Crosstalk between Two bZIP Signaling Pathways Orchestrates Salt-Induced Metabolic Reprogramming in Arabidopsis Roots.
Soil salinity increasingly causes crop losses worldwide. Although roots are the primary targets of salt stress, the signaling networks that facilitate metabolic reprogramming to induce stress tolerance are less understood than those in leaves. Here, a combination of transcriptomic and metabolic approaches was performed in salt-treated Arabidopsis thaliana roots, which revealed that the group S1 basic leucine zipper transcription factors bZIP1 and bZIP53 reprogram primary C- and N-metabolism. In particular, gluconeogenesis and amino acid catabolism are affected by these transcription factors. Importantly, bZIP1 expression reflects cellular stress and energy status in roots. In addition to the well-described abiotic stress response pathway initiated by the hormone abscisic acid (ABA) and executed by SnRK2 (Snf1-RELATED-PROTEIN-KINASE2) and AREB-like bZIP factors, we identify a structurally related ABA-independent signaling module consisting of SnRK1s and S1 bZIPs. Crosstalk between these signaling pathways recruits particular bZIP factor combinations to establish at least four distinct gene expression patterns. Understanding this signaling network provides a framework for securing future crop productivity.
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Laura
L
Hartmann
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Lorenzo
L
Pedrotti
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Christoph
C
Weiste
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Agnes
http://orcid.org/0000-0003-3058-7570
A
Fekete
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Jasper
http://orcid.org/0000-0002-7910-9118
J
Schierstaedt
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Jasmin
J
Göttler
BSIO Berlin School of Integrative Oncology, Charite-Universitätsmedizin Berlin, D-13353 Berlin, Germany.
Stefan
S
Kempa
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Markus
M
Krischke
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Katrin
K
Dietrich
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany.
Martin J
http://orcid.org/0000-0001-8122-5460
MJ
Mueller
Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Campus de Montegancedo, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, Madrid, Spain.
Jesus
http://orcid.org/0000-0002-6332-1712
J
Vicente-Carbajosa
Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands Umeå Plant Science Center, Department of Plant Physiology, Umeå University, SE-901 87 Umeå, Sweden.
Johannes
http://orcid.org/0000-0002-5605-7984
J
Hanson
Julius-von-Sachs-Institut, Pharmazeutische Biologie, Universität Würzburg, 97082 Würzburg, Germany wolfgang.droege-laser@uni-wuerzburg.de.
Wolfgang
W
Dröge-Laser
D000040:Abscisic Acid / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005943:Gluconeogenesis / Q000187:drug effects / Q000235:genetics; D015151:Immunoblotting; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
D000596:Amino Acids; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D010937:Plant Growth Regulators; C492978:SnRK2 protein, Arabidopsis; C553903:bZIP1 protein, Arabidopsis; C544386:bZIP53 protein, Arabidopsis; D012965:Sodium Chloride; D000040:Abscisic Acid; D017346:Protein Serine-Threonine Kinases; D002118:Calcium