In Vitro Biocompatibility of Surface-Modified Porous Alumina Particles for HepG2 Tumor Cells: Toward Early Diagnosis and Targeted Treatment.
Porous alumina photoluminescence-inherent particles are produced and proposed for the development of biomarkers detectors and localized treatment of HepG2 cells. Nanoporous alumina particles (NPAPs) are amorphous, consist of hexagonally ordered nanometric pores in an alumina matrix, have high chemical stability in physiological pH, and exhibit a high inherent photoluminescence in the visible spectrum independently of their size, selectable from nanometers to tens of micrometers. The surface of NPAPs is chemically modified using two different functionalization methods, a multistep method with (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde (GLTA) and a novel simplified-step method with silane-PEG-NHS. Fourier Transform infrared spectroscopy analysis confirmed the proper surface modification of the particles for both functionalization methods. HepG2 cells were cultured during different times with growing concentrations of particles. The analysis of cytotoxicity and cell viability of HepG2 cells confirmed the good biocompatibility of NPAPs in all culture conditions. The results prove the suitability of NPAPs for developing new label-free biomarker detectors and advantageous carriers for localized drug delivery.
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili , Avinguda Països Catalans 26, 43007 Tarragona, Spain.
Elisabet
E
Xifre-Perez
Unitat de Recerca en Lípids i Arteriosclerosi-IISPV, Universitat Rovira i Virgili , C/Sant Llorenç, 21, 43201 Reus, Spain.
Sandra
S
Guaita-Esteruelas
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili , Avinguda Països Catalans 26, 43007 Tarragona, Spain.
Malgorzata
M
Baranowska
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili , Avinguda Països Catalans 26, 43007 Tarragona, Spain.
Josep
J
Pallares
Unitat de Recerca en Lípids i Arteriosclerosi-IISPV, Universitat Rovira i Virgili , C/Sant Llorenç, 21, 43201 Reus, Spain.
Lluis
L
Masana
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili , Avinguda Països Catalans 26, 43007 Tarragona, Spain.
Lluis F
LF
Marsal
D000537:Aluminum Oxide / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D055088:Early Detection of Cancer; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis; D008855:Microscopy, Electron, Scanning; D058608:Nanopores; D011437:Propylamines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D001672:Biocompatible Materials; D004337:Drug Carriers; D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D000537:Aluminum Oxide
biocompatibility; functionalization; in vitro; liver cancer; nanoporous alumina particles; photoluminescence