Effects of aging on cardiac extracellular matrix in men and women.
OBJECTIVE
Aging has severe implications for tissue damage and is a major risk factor for disease. However, the effects of aging on cardiac extracellular matrix (ECM) components in individuals free of cardiovascular disease are incompletely understood. We aimed at the characterization of the effects of aging on major ECM proteins in the heart of men and women.


METHODS
Left ventricular (LV) samples of nondiseased human hearts technically unusable for transplantation obtained from general organ donors (n = 31; age 17-68 years; 48% women) were used for protein isolation. We separated the group into 17-40 years (n = 7 men and 7 women) and 50-68 years (n = 9 men and 8 women).


RESULTS
Analysis of ECM proteins demonstrated an age-dependent sex-specific regulation of collagen type I and III (interaction p < 0.05), type VI (interaction p = 0.01), tissue inhibitor of metalloproteinase 3 (interaction p < 0.05), SMAD2 (interaction p < 0.05), and SMAD3 (interaction p = 0.001). Overall, the levels of these proteins in younger individuals were lower in women than men, while in older individuals they were higher in women than men.


CONCLUSIONS
This age-mediated myocardial ECM remodeling might play a key role in the limited ability of the aging heart to adapt adequately to altered work load and to respond to tissue damage. Therapeutic agents that target ECM homeostasis represent promising prevention strategies.
Institute of Gender in Medicine and Center for Cardiovascular Research, Charite University Hospital, Berlin, Germany.|DZHK (German Centre for Cardiovascular Research), Berlin Partner Site, Berlin, Germany.
Elke
E
Dworatzek
Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary.
Istvan
I
Baczko
Institute of Gender in Medicine and Center for Cardiovascular Research, Charite University Hospital, Berlin, Germany.|DZHK (German Centre for Cardiovascular Research), Berlin Partner Site, Berlin, Germany.
Georgios
G
Kararigas
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism* / Q000473:pathology; D002318:Cardiovascular Diseases / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology
D016326:Extracellular Matrix Proteins
Aging; Extracellular matrix; Heart