[FEATURES OF tRNA GENES AND YOUNG SUBFAMILIES Alu-REPEATS EXPRESSION IN PROLIFERATION AND APOPTOSIS].
Using human U937 cell culture, we studied the expression of the RNA polymerase III-directeci genes--tRNAi(Met)1, tRNA(His) and Alu-repeats belonging to the youngest subfamilies AluY (AluYa5 and AluYb8) - du- ring camptothecin-induced apoptosis. The level of tRNAiMetl increased 1.5-fold, tRNAHis level did not change, and the level of AluY-RNA increased 4-10-fold after 6 hours of CAM treatment compared to control (non-apoptotic) cells. We also studied the level of AluYb8 DNA methylation at apoptosis U937. We have shown that the level of AluYb8 DNA methylation does not change at different stages of apoptosis, and does not differ in apoptotic and control cells. We assume that the increase in gene expression of young AluY repeats and tRNAi(Met)1 plays a role in the apoptosis pathway realization in the cell.
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Kabanov
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Tishchenko
D000470:Alkaloids / Q000494:pharmacology; D020087:Alu Elements; D017209:Apoptosis / Q000187:drug effects; D002166:Camptothecin / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D019175:DNA Methylation; D005786:Gene Expression Regulation; D006801:Humans; D012320:RNA Polymerase III / Q000235:genetics* / Q000378:metabolism; D012354:RNA, Transfer, His / Q000235:genetics* / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D020298:U937 Cells
D000470:Alkaloids; D012354:RNA, Transfer, His; D012358:RNA, Transfer, Met; D012320:RNA Polymerase III; D002166:Camptothecin