In vitro and in vivo comparison of the biological activities of two traditionally and widely used Arum species from Jordan: Arum dioscoridis Sibth & Sm. and Arum palaestinum Boiss.
Arum dioscoridis and A. palaestinum (Araceae) are indigenous plant species in Jordan. HPLC-MS analysis of A. dioscoridis revealed the presence of apigenin, luteolin, quercetin, quercetin-3-O-β-glucoside, vitexin, isoorientin, esculin, and caffeic and ferulic acids. Both Arum spp., influenced gastrointestinal carbohydrate and lipid digestion and absorption. Orlistat inhibited dose dependently and highly substantially pancreatic lipase (PL) in vitro. Similar to orlistat, Arum species aqueous extracts (AEs), apigenin, caffeic acid and esculin exhibited a concentration related PL inhibition. Comparable to acarbose, dual inhibition of α-amylase/α-glucosidase was observed for both Arum species. Like guar gum, A. dioscoridis AE minimised substantially area under 24 h glucose curve. Acute starch-induced postprandial hyperglycaemia in overnight fasting rats was highly significantly (p < 0.001) decreased by A. dioscoridis AE. A. palaestinum could not perform effectively in either starch- or glucose-fed fasting rats. No antiproliferative effects against colorectal cancer cell lines HT29, HCT116 and SW620 were detected for tested Arum spp.
a Faculty of Pharmacy , The University of Jordan , Amman , Jordan.
Fatma U
FU
Afifi
a Faculty of Pharmacy , The University of Jordan , Amman , Jordan.
Violet
V
Kasabri
b National Institute for Biological Sciences , Bucharest , Romania.
Simona C
SC
Litescu
a Faculty of Pharmacy , The University of Jordan , Amman , Jordan.
Ismail M
IM
Abaza
D020909:Acarbose / Q000494:pharmacology; D000818:Animals; D031058:Arum / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy; D007597:Jordan; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D000516:alpha-Amylases / Q000037:antagonists & inhibitors; D000520:alpha-Glucosidases
D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D020909:Acarbose
A. palaestinum Boiss.; Araceae; Arum dioscoridis Sibth & Sm.; HPLC-MS; pancreatic lipase and α-amylase