Effect of subarachnoid nerve block anesthesia on glutamate transporter GLAST and GLT-1 expressions in rabbits.
OBJECTIVE
To observe the effect of subarachnoid nerve block anesthesia on glutamate transporter glutamate-aspartate transporter (GLAST) and GLT-1 expressions in rabbits, and to investigate the effect of peripheral nerve anesthesia on the morphology and function of the spinal cord.


METHODS
Twenty healthy New Zealand white rabbits were randomly divided into two groups: the experimental group and control group; with 10 rabbits in each group. For spinal nerve anesthesia, 5 g/L of bupivacaine was used in the experimental group, and sterile saline was used in the control group. After 30 min of cardiac perfusion, GLAST and GLT-1 protein expression in spinal neurons were detected by immunohistochemistry and immunofluorescence staining.


RESULTS
GLAST and GLT-1 protein-positive cells increased in neurons in the experimental group, compared with the control group (P < 0.05).


CONCLUSIONS
After subarachnoid nerve block anesthesia, rabbit glutamate transporter GLAST and GLT-1 expression is increased; and spinal cord nerve cell function is inhibited.
Department of Anesthesiology, Shandong Provincial Hospital, Jinan, Shandong 250031, China. Electronic address: Doctorxiao1115@foxmail.com.
Ke-Qing
KQ
Xiao
Division of Preventive Medicine, Third People's Hospital of Pingdu City, Qingdao, Shandong 266753, China.
Mei
M
Xiao
Department of Anesthesiology, Shandong Provincial Hospital, Jinan, Shandong 250031, China.
Li
L
Meng
Department of Anesthesiology, Shandong Provincial Hospital, Jinan, Shandong 250031, China.
Xiang-Yang
XY
Du
Department of Anesthesiology, Shandong Provincial Hospital, Jinan, Shandong 250031, China.
Jing
J
Hu
Department of Anesthesiology, Shandong Provincial Hospital, Jinan, Shandong 250031, China.
Bao-Feng
BF
Gao
Department of Anesthesiology, Shandong Provincial Hospital, Jinan, Shandong 250031, China.
Wen-Qiang
WQ
Yu
Shandong Province Chest Hospital, Department of Respiratory Diseases of Jinan City, Shandong 250 013, China.
Xin-Jie
XJ
Wang
Department of Anesthesiology, Qianfoshan Hospital Affiliated to Shandong University in Shandong Province, Jinan, Shandong 250014, China.
Yan-Lin
YL
Ban
GLAST; GLT-1; Glutamate transporter; Subarachnoid nerve block anesthesia