A Rationally Designed Upconversion Nanoprobe for in Vivo Detection of Hydroxyl Radical.
The detection of •OH in live organisms is crucial to the understanding of its physiological and pathological roles; while this is too challenging because of the extremely low concentration and high reactivity of the species in the body. Herein, we report the rational design and fabrication of an NIR-light excited luminescence resonance energy transfer-based nanoprobe, which for the first time realizes the in vivo detection of •OH. The nanoprobe is composed of two moieties: upconversion nanoparticles with sandwich structure and bared surface as the energy donor; and mOG, a modified azo dye with tunable light absorption, as both the energy acceptor and the •OH recognizing ligand. The as-constructed nanoprobe exhibited ultrahigh sensitivity (with the quantification limit down to 1.2 femtomolar, several orders of magnitude lower than that of most previous •OH probes), good biocompatibility, and specificity. It was successfully used for monitoring [•OH] levels in live cells and tissues.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Zhen
Z
Li
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Tao
T
Liang
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Songwei
S
Lv
Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States.
Qinggeng
Q
Zhuang
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Zhihong
Z
Liu
D000818:Animals; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis*; D008024:Ligands; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008099:Liver / Q000737:chemistry; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*
D005456:Fluorescent Dyes; D008024:Ligands; D008070:Lipopolysaccharides; D017665:Hydroxyl Radical