Expression of bioactive recombinant human fibroblast growth factor 9 in oil bodies of Arabidopsis thaliana.
Fibroblast growth factor 9 (FGF9) has autocrine and paracrine functions in chondrogenesis osteogenesis, hair growth, and gonadal differentiation. We have expressed recombinant human FGF9 (rhFGF9) in the oil bodies of Arabidopsis thaliana via the floral dip method. The expression vector pOTB-rhFGF9 contained an oleosin-rhFGF9 fusion gene and a glufosinate resistance gene for selection. This plasmid was transformed into A. thaliana and expression of the fusion protein oleosin-rhFGF9 confirmed by SDS-PAGE and Western blotting. Furthermore, MTT assays demonstrated that the oil bodies expressed oleosin-rhFGF9 from the transgenic A. thaliana had a remarkable proliferation effect on NIH/3T3 cells.
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, Jilin, China.
Shanyong
S
Yi
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Jing
J
Yang
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, Jilin, China.
Jian
J
Huang
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Lili
L
Guan
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Linna
L
Du
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Yongxin
Y
Guo
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Feng
F
Zhai
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Yanfang
Y
Wang
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Zhen
Z
Lu
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Liyong
L
Wang
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
Haiyan
H
Li
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China. Electronic address: xiaokunli@163.net.
Xiaokun
X
Li
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China. Electronic address: changjiang10@hotmail.com.
Chao
C
Jiang
D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D049109:Cell Proliferation; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D051525:Fibroblast Growth Factor 9 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D030821:Plants, Genetically Modified / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins; C496459:FGF9 protein, human; D051525:Fibroblast Growth Factor 9; D011993:Recombinant Fusion Proteins; C079353:oleosin protein, Arabidopsis
Arabidopsis thaliana; Fusion protein expression; Human fibroblast growth factor 9; Oil body