General Cytotoxicity Assessment by Means of the MTT Assay.
Cytotoxicity assays were among the first in vitro bioassay methods used to predict toxicity of substances to various tissues. In vitro cytotoxicity testing provides a crucial means for safety assessment and screening, and for ranking compounds. The choice of using a particular cytotoxicity assay technology may be influenced by specific research goals. As such, four main classes of assays are used to monitor the response of cultured cells after treatment with potential toxicants. These methods measure viability, cell membrane integrity, cell proliferation, and metabolic activity. In this chapter, we focus on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction colorimetric assay to evaluate detrimental intracellular effects on metabolic activity. This assay is well-characterized, simple to use and remains popular in several laboratories worldwide.
Unidad de Hepatología Experimental, Instituto de Investigación Sanitaria La Fe, Torre A, Avda Fernando Abril Martorell 106, 46026, Valencia, Spain.
Laia
L
Tolosa
María Teresa
MT
Donato
María José
MJ
Gómez-Lechón
D000818:Animals; D041702:BALB 3T3 Cells; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry / Q000379:methods*; D022781:Hepatocytes / Q000378:metabolism; D051379:Mice; D051381:Rats; D013778:Tetrazolium Salts / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism*; D018675:Toxicity Tests
D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue