High-level Multi-Resistant and Virulent Escherichia coli in Abeokuta, Nigeria.
Multi-resistant Escherichia coli (E. coli) strains co-harboring virulence genes is a cause of high morbidity in Abeokuta, Nigeria. This study was designed to determine some virulent factors among enteropathogenic E. coli in Abeokuta, Nigeria. Approximately non-repetitive 102 isolates of E. coli were recovered from clinical samples from two health facilities in Abeokuta. Biotyping using API and antibiotic susceptibility was determined, and eae and flic genes were assayed by PCR. Antibiotic resistance relatedness was performed by DendroUPGMA. Results showed that 48.0% and 52.0 % were intestinal and extra-intestinal E. coli, ampicillin recorded 100% resistance, amoxycilli/clavulanic acid 64.7%, cotrimoxazole 57.8% and 56.8% resistance against cefotaxime, at MIC >16 ug/mL, 100%, 57.8%, and 50% have MIC50 to ampicillin, tetracycline, and ceftazidime, while 74.5% and 48.0% have MIC90 to ampicillin and ceftazidime. Significant rates of 4.9%, 7.8%, and 9.8% flic, eae, and flic/eae genes were found in intestinal isolates, while 2.9%, 2.0%, and 3.9% were found in extra-intestinal (P < 0.05). Two major clades of the resistant isolates reveal significant antibiotic relatedness among intestinal and extra-intestinal isolates, at 54% resistance similarities with very high multi-antibiotic resistance index of 1.0 (MARI). A high rate of undetected virulent E. coli pathotypes with high resistance could trigger unprecedented morbidity and mortality, mostly among children and the elderly.
a Department of Medical Microbiology & Parasitology , Olabisi Onabanjo University , Ogun State , Nigeria.
Paul Akinniyi
PA
Akinduti
b Department of Pathology , Federal Medical Center , Abeokuta , Ogun State , Nigeria.
Bukola W
BW
Aboderin
c Biotechnology Centre , Federal University of Agriculture , Abeokuta , Ogun State , Nigeria.
Rasaq
R
Oloyede
b Department of Pathology , Federal Medical Center , Abeokuta , Ogun State , Nigeria.
Joseph I
JI
Ogiogwa
b Department of Pathology , Federal Medical Center , Abeokuta , Ogun State , Nigeria.|d Department of Virology , College of Medicine, University of Ibadan , Ibadan , Nigeria.
Babatunde O
BO
Motayo
a Department of Medical Microbiology & Parasitology , Olabisi Onabanjo University , Ogun State , Nigeria.
Oluwaseun
O
Ejilude
D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009549:Nigeria / Q000453:epidemiology; D037521:Virulence Factors / Q000235:genetics*
D037521:Virulence Factors
Escherichia coli; antibiotics; eae; flic