NMR-Based Metabolomic Analysis of Huanglongbing-Asymptomatic and -Symptomatic Citrus Trees.
Huanglongbing (HLB) is one of the most severe diseases that affects citrus trees worldwide and is associated with the yet uncultured bacteria Candidatus Liberibacter spp. To assess the metabolomic differences between HLB-asymptomatic and -symptomatic tissues, extracts from leaf and root samples taken from a uniform 6-year-old commercial orchard of Valencia trees were subjected to nuclear magnetic resonance (NMR) and chemometrics. The results show that the symptomatic trees had higher sucrose content in their leaves and no variation in their roots. In addition, proline betaine and malate were detected in smaller amounts in the HLB-affected symptomatic leaves. The changes in metabolic processes of the plant in response to HLB are corroborated by the relationship between the bacterial levels and the metabolic profiles.
Universidade Federal de Mato Grosso do Sul (UFMS) , Instituto de Quı́mica, CP 549, CEP 79.074-460, Campo Grande, MS, Brazil.
Deisy dos Santos
Ddos S
Freitas
Instituto Agronômico do Paraná (IAPAR) , Laboratório de Biotecnologia Vegetal, CP 481, CEP 86.001-970, Londrina, PR, Brazil.
Eduardo Fermino
EF
Carlos
Instituto Agronômico de Campinas (IAC) , Laboratório de Biotecnologia, CP 04, CEP 13.490-970, Cordeirópolis, SP, Brazil.
Márcia Cristina Soares de Souza
MC
Gil
Universidade do Oeste Paulista (UNOESTE) , Rodovia Raposo Tavares, km 572, CEP 19.067-175, Presidente Prudente, SP, Brazil.
Luiz Gonzaga Esteves
LG
Vieira
Universidade Federal de Mato Grosso do Sul (UFMS) , Instituto de Quı́mica, CP 549, CEP 79.074-460, Campo Grande, MS, Brazil.
Glaucia Braz
GB
Alcantara
D002957:Citrus / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012230:Rhizobiaceae / Q000502:physiology; D014197:Trees / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology
Candidatus Liberibacter; NMR; chemometrics; citrus greening disease; huanglongbing (HLB); plant response