Reaction-Based "Off-On" Fluorescent Probe Enabling Detection of Endogenous Labile Fe(2+) and Imaging of Zn(2+)-induced Fe(2+) Flux in Living Cells and Elevated Fe(2+) in Ischemic Stroke.
Fluorogenic sensors capable of spatiotemporally detecting Fe(2+) in biological systems are highly valuable in the study of iron biology. Toward this end, a new "off-on" Fe(2+)-selective fluorescent probe has been developed by incorporating an Fe(2+)-induced N-O cleavage of acylated hydroxylamine moiety into the naphthalimide fluorophore. The probe displays facile response (within 15 min) and good selectivity toward Fe(2+) with >27-fold enhancement of fluorescence intensity and high sensitivity of as low as 0.5 μM with a noticeable 3-fold fluorescence enhancement. These features of the probe have been transformed into in the convenient detection of endogenous, basal level of labile Fe(2+) pools in living cells. Furthermore, we have demonstrated the capacity of the probe for the studies of important Fe(2+) related biological functions. It can respond to the Zn(2+)-induced Fe(2+) flux, an important event observed in stroke, and facilely detect the elevated level of Fe(2+) in the brain tissue of a rat undergoing ischemic stroke at the ischemic site.
Department of Chemistry & Chemical Biology, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Weimin
W
Xuan
Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Rong
R
Pan
Department of Chemistry & Chemical Biology, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Yongyi
Y
Wei
Department of Chemistry & Chemical Biology, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Yanting
Y
Cao
Department of Chemistry & Chemical Biology, University of New Mexico , Albuquerque, New Mexico 87131, United States.|College of Pharmaceutical Science, Soochow University , Suzhou 215123, P. R. China.
Huanqiu
H
Li
Department of Chemistry & Chemical Biology, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Fu-Sen
FS
Liang
Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Ke-Jian
KJ
Liu
Department of Chemistry & Chemical Biology, University of New Mexico , Albuquerque, New Mexico 87131, United States.
Wei
W
Wang
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D007501:Iron / Q000032:analysis* / Q000378:metabolism; D008297:Male; D053644:Naphthalimides / Q000737:chemistry* / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000378:metabolism* / Q000473:pathology; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
D005456:Fluorescent Dyes; D053644:Naphthalimides; D007501:Iron; D015032:Zinc