Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice.
The current study was carried out to investigate the protective role of biotin in kidney injury and oxidative stress in diabetic mice type 1. Male Swiss albino mice were randomly divided into 3 groups. Control group received saline. Diabetes type 1 was induced in second and third groups by intraperitoneal injection of streptozotocin as a single dose (150 mg/kg). Second group remained as the untreated diabetic group and the third group received 15 mg/kg daily oral dose of biotin for 12 successive days. Biochemical results showed significant elevation in blood glucose and urea levels in both diabetic groups. Also, there is an increase in glomerular areas and decrease in glomerular cellularity in both diabetic groups. Histopathological results showed severe alterations in the untreated diabetic group represented by distorted glomeruli, inflammatory cells, and giant macrophages. In addition, there was an intense immune-reaction response toward acrolein indicator of oxidative damage. Upon biotin administration of diabetic mice, the above mentioned histopathological changes were reduced and also acroline reaction of oxidative damage was diminished. Our findings prove that biotin has a protective role against streptozotocin-induced oxidative damage in kidneys of laboratory mice.
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Badr A
BA
Aldahmash
Department of Zoology, College of Girls for Science, Arts and Education, Ain Shams University, Cairo, Egypt.
Doaa M
DM
El-Nagar
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Khalid E
KE
Ibrahim
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Mahmoud S
MS
Metwaly
Biotin; Diabetes; Mice; Nephroprotective; Oxidative damage; Streptozotocin