The effect of vitamin E and L-carnitine against methotrexate-induced injury in rat testis.
OBJECTIVE
Methotrexate (MTX), used commonly as an antimetabolite drug in cancer therapy, leads to acute toxic side effects in tissues or organs containing rapidly dividing cells, such as bone marrow, gastrointestinal mucosa, and seminiferous tubules. In this study, we investigated the protective effects of vitamin E and L-carnitine against MTX-induced injury in rat testis.


METHODS
Rats were divided into 4 groups, including the control group. The study took 17 days and the animals received daily doses of 0.5 mL/kg saline, 250 mg/kg vitamin E, or 500 mg/kg L-carnitine intraperitoneally. The experimental groups received 20 mg/kg methotrexate intraperitoneally on days 3 and 10.


RESULTS
Weight loss, testicular weight loss and marked histological injuries, increased malondialdehyde levels, and decreased superoxide dismutase levels were only observed in the MTX-treated groups. Vitamin E and L-carnitine treatments did not affect MTX-induced weight loss or testicular weight loss, but they inhibited MTX-induced testicular histological injuries. Vitamin E and L-carnitine treatments suppressed the increases in malondialdehyde levels and the decreases in superoxide dismutase levels.


CONCLUSIONS
Vitamin E and L-carnitine treatments decreased MTX-induced testicular histological injuries, and these results were supported by biochemical measurements.
Mehmet
M
Yüncü
Nezahat
N
Bükücü
Nuray
N
Bayat
Leman
L
Sencar
Mehmet
M
Tarakçioğlu
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D002331:Carnitine / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008727:Methotrexate; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013733:Testicular Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D014803:Vitamin B Complex / Q000627:therapeutic use*; D014810:Vitamin E / Q000378:metabolism / Q000627:therapeutic use*; D015431:Weight Loss
D000975:Antioxidants; D014803:Vitamin B Complex; D014810:Vitamin E; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D002331:Carnitine; D008727:Methotrexate