The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel).
The vitellogenin receptor (VgR) functions as an essential component in uptaking and transporting vitellogenin (Vg) in female adults, which is involved in ovary development and oviposition. This study aimed to clarify the molecular characteristics and function of VgR in the oriental fruit fly Bactrocera dorsalis (Hendel). Here, we identified the full-length of BdVgR (GenBank Accession No. JX469118), encoding a 1925 residue (aa) protein with a 214.72 kDa molecular mass and several typical motifs of low-density lipoprotein receptor superfamily (LDLR). Phylogenic analysis suggested that BdVgR was evolutionary conserved with other Dipteran VgRs. The expression of BdVgR was exclusively detected in the ovaries rather than head, thorax or other tissues. The developmental expression patterns showed that the signal of BdVgR was detectable in very beginning of adult stage, and positively correlated with the growth rate of ovaries and the expression levels of its ligands. In addition, we also demonstrated that the expression level of BdVgR, and ovary development were significantly suppressed after being injected with BdVgR-targeted dsRNA. Together, all of these results indicated that BdVgR was critical for yolk protein absorption and ovary maturation in B. dorsalis, playing a vital role in female reproduction.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. ranchun@cric.cn.|Citrus Research Institute, Southwest University, Chongqing 400712, China. ranchun@cric.cn.
Lin
L
Cong
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. yangwenjia10@126.com.|College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, China. yangwenjia10@126.com.
Wen-Jia
WJ
Yang
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. jxzzby@163.com.
Xuan-Zhao
XZ
Jiang
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. jinzhiniu@yahoo.com.
Jin-Zhi
JZ
Niu
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. blackaet@163.com.
Guang-Mao
GM
Shen
Citrus Research Institute, Southwest University, Chongqing 400712, China. ranchun@cric.cn.
Chun
C
Ran
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. wangjinjun@swu.edu.cn.
Jin-Jun
JJ
Wang
D000818:Animals; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D033621:Tephritidae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism*
D004527:Egg Proteins; D019476:Insect Proteins; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D014819:Vitellogenins; C030474:vitellogenin receptor
Bactrocera dorsalis; RNA interference; ovary; vitellogenin receptor; yolk protein