Industrial wastewater as raw material for exopolysaccharide production by Rhizobium leguminosarum.
The objective of this study was to evaluate the exopolysaccharide (EPS) production by Rhizobium leguminosarum cultivated in wastewater generated by oil companies (WWOC1 and WWOC2) and fish processing industry (WWFP). The results obtained in Erlenmeyer flasks indicated that the rhizobial strain grew well in industrial wastewater. Generally, wastewater composition affected the growth and the EPS production. WWFP allowed good bacterial growth similar to that obtained with the standard medium (YMB). During growth, various quantities of EPS were produced and yields varied depending on the media. Growing in YMB, EPS production did not exceed 9.7 g/L obtained after 72 h of growth. In wastewater, the maximum EPS value reached 11.1 g/L obtained with the fish processing wastewater, after 72 h of growth. The use of a mixture of the oil company wastewater (WWOC2) and the fish processing wastewater (WWFP) as culture medium affected not only the rhizobial strain growth, but also EPS production. The highest EPS (42.4 g/L, after 96 h of culture) was obtained using a ratio of WWFP and WWOC2 of 50:50 (v:v). Therefore, this work shows the ability of Rhizobium leguminosarum, growing in industrial wastewater as new economic medium, to produce EPS. This biopolymer could be applied in enormous biotechnological areas.
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia.
Mohamed
M
Sellami
Instytut Badawczy Lesnictwa, Forest Research Institute, Sekocin Stary, ulica Raszyn, Poland, Instytut Badawczy Lesnictwa, Forest Research Institute, Sekocin Stary, ulica Raszyn, Poland.
Tomasz
T
Oszako
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia.
Nabil
N
Miled
King Khaled University, King Khalid University, Community College at Khamis Mushait, Khamis Mushait, Saudi Arabia, King Khalid University, Community College at Khamis Mushait, Khamis Mushait, Saudi Arabia.
Faouzi
F
Ben Rebah
D019649:Food Industry; D007220:Industrial Waste; D000066388:Oil and Gas Industry; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D016961:Rhizobium leguminosarum / Q000254:growth & development* / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology*
D007220:Industrial Waste; D011135:Polysaccharides, Bacterial; D062065:Wastewater
Rhizobium; biopolymer; exopolysaccharide; industrial wastewater