Angelica Sinensis attenuates inflammatory reaction in experimental rat models having spinal cord injury.
This study was aimed to evaluate the effect of Angelica Sinensis on experimental rat models in which spinal cord injury was induced by studying different factors. Different factors causing inflammation play a key role in pathophysiology of SCI. Here three groups of rats (n=15, each was used). These included a sham control group where only laminectomy was performed, SCI group where SCI was induced and AS/SCI group where although SCI was induced but Angelica Sinensis was also administered to study its effect and draw a comparison with control. The expression of I-kBα and NF-kB p65 was also studied using western blotting and after recording optical density (OD) values of western blots. MPO activity was used to measure the effect of 20 mg/kg Angelica Sinensis. The levels of proinflammatory cytokines TNF-α, IL-1β and IL-6 were also studied. As compared with SCI group and sham control it was observed that Angelica Sinensis significantly reduced the expression of I-kBα and NF-kB p65, (P<0.05), while MPO activity was also significantly reduced. Proinflammatory cytokine level was also reduced in treated group as compared to both other groups. On the basis of this study we concluded that the use of 20 mg/kg Angelica Sinensis in rat models can attenuate the secondary damage caused by SCI and thus help in controlling the pathology of SCI in rats.
Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Jun
J
Xu
Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University Harbin 150081, China.
Xiao-Qiang
XQ
E
Department of Orthopedics, Hulan District Hospital of Traditional Chinese Medicine Hulan 150500, China.
Hui-Yong
HY
Liu
Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Jun
J
Tian
Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Jing-Long
JL
Yan
D029971:Angelica sinensis / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D009187:Myelitis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000072000:NF-KappaB Inhibitor alpha; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010946:Plants, Medicinal; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051996:Transcription Factor RelA / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D020672:I-kappa B Proteins; D018836:Inflammation Mediators; C000606206:Nfkbia protein, rat; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D000072000:NF-KappaB Inhibitor alpha; C044613:angelicae sinensis extract; D009195:Peroxidase
MPO activity; Spinal cord injury; angelica sinensis; inflammation in SCI; proinflammatory cytokines