Sodium Para-aminosalicylic Acid Protected Primary Cultured Basal Ganglia Neurons of Rat from Manganese-Induced Oxidative Impairment and Changes of Amino Acid Neurotransmitters.
Manganese (Mn), an essential trace metal for protein synthesis and particularly neurotransmitter metabolism, preferentially accumulates in basal ganglia. However, excessive Mn accumulation may cause neurotoxicity referred to as manganism. Sodium para-aminosalicylic acid (PAS-Na) has been used to treat manganism with unclear molecular mechanisms. Thus, we aim to explore whether PAS-Na can inhibit Mn-induced neuronal injury in basal ganglia in vitro. We exposed basal ganglia neurons with 50 μM manganese chloride (MnCl2) for 24 h and then replaced with 50, 150, and 450 μM PAS-Na treatment for another 24 h. MnCl2 significantly decreased cell viability but increased leakage rate of lactate dehydrogenase and DNA damage (as shown by increasing percentage of DNA tail and Olive tail moment). Mechanically, Mn reduced glutathione peroxidase and catalase activity and interrupted amino acid neurotransmitter balance. However, PAS-Na treatment reversed the aforementioned Mn-induced toxic effects. Taken together, these results showed that PAS-Na could protect basal ganglia neurons from Mn-induced neurotoxicity.
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Shao-Jun
SJ
Li
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Yong
Y
Li
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Jing-Wen
JW
Chen
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Zong-Xiang
ZX
Yuan
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Yu-Huan
YH
Mo
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Guo-Dong
GD
Lu
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China. ymjianggxmu@163.com.
Yue-Ming
YM
Jiang
Department of Toxicology, School of Public Health, Guilin Medical University, Guilin, 541004, China.
Chao-Yan
CY
Ou
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Fang
F
Wang
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Xiao-Wei
XW
Huang
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Yi-Ni
YN
Luo
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Shi-Yan
SY
Ou
Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-yong Rd., Nanning, 530021, Guangxi, China.
Yan-Ni
YN
Huang
D010131:Aminosalicylic Acid / Q000494:pharmacology*; D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D008345:Manganese / Q000633:toxicity*; D020149:Manganese Poisoning / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley
D018377:Neurotransmitter Agents; D008345:Manganese; D010131:Aminosalicylic Acid
Amino acid neurotransmitters; Basal ganglia neuron; Manganese; Oxidative stress; Sodium para-aminosalicylic acid