Fe(II)-Co(II) Double Salt Incorporated Magnetic Hydrophobic Microparticles for Invertase Adsorption.
Invertase (β-fructofuranoside fructohydrolase, EC 3.2.1.26) is an enzyme widely used in the food industry. Its main function is the formation of glucose and fructose through hydrolysis of sucrose. For the separation and purification of this commercially important enzyme from aqueous solutions, magnetic poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), m-poly(HEMA-MATrp) microparticles were developed. Magnetic properties of microparticles are provided using ferromagnetic Fe(II)-Co(II) double salt. Characterization studies of magnetic microparticles were conducted via vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. Specific surface area of magnetic microparticles is 6.75 m(2)/g. Because of all experiments performed in this study, the adsorption capability of magnetic microparticles was optimized by variation of different conditions (pH, interaction time, initial invertase concentration, temperature, and ionic strength) and maximum adsorption capacity was determined as 992.64 mg invertase/g magnetic microparticles.
Scientific Technical Research and Application Center, Hitit University, Çorum, Turkey.
Kazım
K
Köse
Faculty of Science and Literature, Department of Chemistry, Hitit University, Çorum, Turkey. kadirerol86@gmail.com.
Kadir
K
Erol
Institute of Science, Department of Biology, Hitit University, Çorum, Turkey.
Asiye Aslı
AA
Emniyet
Faculty of Science and Literature, Department of Chemistry, Hitit University, Çorum, Turkey.
Dursun Ali
DA
Köse
Faculty of Science-Literature, Department of Molecular Biology and Genetics, Hitit University, Çorum, Turkey.
Gülçin Alp
GA
Avcı
Faculty of Science, Department of Chemistry, Hacettepe University, Ankara, Turkey.
Lokman
L
Uzun
D003035:Cobalt / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D008280:Magnetics; D011073:Polyamines / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011073:Polyamines; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate; D003035:Cobalt; D013395:Sucrose; D007501:Iron; D043324:beta-Fructofuranosidase
Adsorption; Double salt; Hydrophobic microparticles; Invertase; Magnetic separation