Shen-Fu Injection () alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca(2+)-ATPase.
OBJECTIVE
To compare the effect of Shen-Fu Injection (SFI) and epinephrine on the expression of sarcoplasmic reticulum Ca(2+) ATPase 2a (SERCA2a) in a pig model with post-resuscitation myocardial dysfunction.


METHODS
Ventricular fibrillation (VF) was electrically induced in Wu-zhi-shan miniature pigs. After 8 min of untreated VF and 2 min of cardiopulmonary resuscitation (CPR), all animals were randomly administered a bolus injection of saline placebo (SA group, n=10), SFI (0.8 mg/kg, SFI group, n=10) or epinephrine (20 μg/kg, EPI group, n=10). After 4 min of CPR, a 100-J shock was delivered. If the defibrillation attempt failed to attain restoration of spontaneous circulation (ROSC), manual chest compressions were rapidly resumed for a further 2 min followed by a second defibrillation attempt. Hemodynamic variables were recorded, and plasma concentrations of catecholamines were measured. Adenylate cyclase (AC), cyclic adenosine monophosphate (cAMP) and the expressions of β1-adrenoceptor (AR) and SERCA 2a were determined.


RESULTS
Cardiac output, left ventricular dp/dtmax and negative dp/dtmax were significantly higher in the SFI group than in the SA and EPI groups at 4 and 6 h after ROSC. The expression of β1-AR and SERCA2a at 24 h after ROSC were significantly higher in the SFI group than in the SA and EPI groups (P<0.05 or P<0.01).


CONCLUSIONS
The administration of epinephrine during CPR decreased the expression of SERCA2a and aggravated postresuscitation myocardial function (P<0.01). SFI attenuated post-resuscitation myocardial dysfunction, and the mechanism might be related to the up-regulation of SERCA2a expression.
Intensive Care Unit, Shandong Cancer Hospital and Institute, Jinan, 250117, China.|Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
Zhi-Jun
ZJ
Guo
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
Cai-Jun
CJ
Wu
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China. lcscyyy@163.com.
Chun-Sheng
CS
Li
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002302:Cardiac Output / Q000187:drug effects; D016887:Cardiopulmonary Resuscitation; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D004837:Epinephrine / Q000097:blood; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D006439:Hemodynamics / Q000187:drug effects; D007267:Injections; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology*; D009638:Norepinephrine / Q000097:blood; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature; D015854:Up-Regulation / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D018342:Receptors, Adrenergic, beta-1; C492793:Shen-Fu; D000242:Cyclic AMP; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000262:Adenylyl Cyclases; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine
Chinese medicine; Shen-fu Injection; cardiac arrest; cardiopulmonary resuscitation; epinephrine; sarcoplasmic reticulum Ca2+ ATPase 2a