Rapid 3D Extrusion of Synthetic Tumor Microenvironments.
The spatial positioning of 3D tumor-mimetic microenvironments, containing multiple cell types, is controlled with a simple concentric flow device in a single step. A range of geometric architectures are demonstrated, and the migration of segregated tumor cells and macrophages is explored using drugs that inhibit heterotypic interactions.
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Joshua M
JM
Grolman
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Douglas
D
Zhang
Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Andrew M
AM
Smith
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.|Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Jeffrey S
JS
Moore
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.|Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Kristopher A
KA
Kilian
D000230:Adenocarcinoma / Q000188:drug therapy / Q000503:physiopathology; D000464:Alginates / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry; D001943:Breast Neoplasms / Q000188:drug therapy / Q000503:physiopathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D018920:Coculture Techniques; D004867:Equipment Design; D020723:Glucuronic Acid / Q000138:chemical synthesis / Q000737:chemistry; D006603:Hexuronic Acids / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds; D059016:Tumor Microenvironment / Q000187:drug effects* / Q000502:physiology*
D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid
microfluidics; patterned hydrogels; tissue engineering; tumor microenvironments