Maternal group B Streptococcus and the infant gut microbiota.
Early patterns of gut colonization may predispose children to adult disease. Exposures in utero and during delivery are associated with the infant gut microbiome. Although ~35% of women carry group B strep (GBS; Streptococcus agalactiae) during pregnancy, it is unknown if GBS presence influences the infant gut microbiome. As part of a population-based, general risk birth cohort, stool specimens were collected from infant's diapers at research visits conducted at ~1 and 6 months of age. Using the Illumina MiSeq (San Diego, CA) platform, the V4 region of the bacterial 16S rRNA gene was sequenced. Infant gut bacterial community compositional differences by maternal GBS status were evaluated using permutational multivariate analysis of variance. Individual operational taxonomic units (OTUs) were tested using a zero-inflated negative binomial model. Data on maternal GBS and infant gut microbiota from either 1 (n=112) or 6-month-old stool (n=150) specimens was available on 262 maternal-child pairs. Eighty women (30.5%) were GBS+, of who 58 (72.5%) were given intrapartum antibiotics. After adjusting for maternal race, prenatal antifungal use and intrapartum antibiotics, maternal GBS status was statistically significantly associated with gut bacterial composition in the 6 month visit specimen (Canberra R 2=0.008, P=0.008; Unweighted UniFrac R 2=0.010, P=0.011). Individual OTU tests revealed that infants of GBS+ mothers were significantly enriched for specific members of the Clostridiaceae, Ruminococcoceae, and Enterococcaceae in the 6 month specimens compared with infants of GBS- mothers. Whether these taxonomic differences in infant gut microbiota at 6 months lead to differential predisposition for adult disease requires additional study.
1Department of Public Health Sciences,Henry Ford Hospital,One Ford Place,Detroit,MI,USA.
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http://orcid.org/0000-0001-8272-4448
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Cassidy-Bushrow
1Department of Public Health Sciences,Henry Ford Hospital,One Ford Place,Detroit,MI,USA.
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Sitarik
1Department of Public Health Sciences,Henry Ford Hospital,One Ford Place,Detroit,MI,USA.
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Levin
3Department of Medicine,University of California,San Francisco,CA,USA.
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Lynch
1Department of Public Health Sciences,Henry Ford Hospital,One Ford Place,Detroit,MI,USA.
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Havstad
2Center for Allergy,Asthma and Immunology Research,Henry Ford Hospital,Detroit,MI,USA.
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Ownby
1Department of Public Health Sciences,Henry Ford Hospital,One Ford Place,Detroit,MI,USA.
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Johnson
1Department of Public Health Sciences,Henry Ford Hospital,One Ford Place,Detroit,MI,USA.
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Wegienka
D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D015331:Cohort Studies; D005260:Female; D000069196:Gastrointestinal Microbiome; D006801:Humans; D007231:Infant, Newborn; D008875:Middle Aged; D011247:Pregnancy; D013290:Streptococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013292:Streptococcus agalactiae; D055815:Young Adult
D000900:Anti-Bacterial Agents
antibiotics; developmental origins; group B strep; gut microbiota