A novel detection technique of polyamide binding sites by photo-induced electron transfer in (Br)U substituted DNA.
We report the photochemistry of (Br)U substituted DNA as a versatile platform to investigate the binding sites of pyrene conjugated pyrrole imidazole polyamides (PIPs). The results suggest that the approach can be used on a routine basis for the screening of polyamide binding sites.
Department of Science, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8501, Japan. hs@kuchem.kyoto-u.ac.jp.
Abhijit
A
Saha
Fumitaka
F
Hashiya
Seiichiro
S
Kizaki
Sefan
S
Asamitsu
Kaori
K
Hashiya
Toshikazu
T
Bando
Hiroshi
H
Sugiyama
D001665:Binding Sites; D001976:Bromouracil / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004583:Electrons; D009757:Nylons / Q000737:chemistry*
D009757:Nylons; D001976:Bromouracil; D004247:DNA