A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation.
A smart device for label-free and real-time detection of gene point mutation-related diseases was developed based on the high dark phase contrast of vapor condensation. The main components of the device included a Peltier cooler and a mini PC board for image processing. Heat from the hot side of the Peltier cooler causes the fluid in a copper chamber to evaporate, and the vapor condenses on the surface of a microarray chip placed on the cold side of the cooler. The high dark phase contrast of vapor condensation relative to the analytes on the microarray chip was explored. Combined with rolling circle amplification, the device visualizes less-to-more hydrophilic transitions caused by gene trapping and DNA amplification. A lung cancer gene point mutation was analysed, proving the high selectivity and multiplex analysis capability of this low-cost device.
Department of Biomedical Engineering, Tsinghua University School of Medicine, Beijing 100084, China. tshgl@ tsinghua.edu.cn.
Junqi
J
Zhang
Rongxin
R
Fu
Liping
L
Xie
Qi
Q
Li
Wenhan
W
Zhou
Ruliang
R
Wang
Jiancheng
J
Ye
Dong
D
Wang
Ning
N
Xue
Xue
X
Lin
Ying
Y
Lu
Guoliang
G
Huang
D004273:DNA, Neoplasm / Q000032:analysis*; D005740:Gases / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D017354:Point Mutation; D014867:Water / Q000737:chemistry
D004273:DNA, Neoplasm; D005740:Gases; D014867:Water