Adenosine Triphosphate-Triggered Release of Macromolecular and Nanoparticle Loads from Aptamer/DNA-Cross-Linked Microcapsules.
The synthesis of stimuli-responsive DNA microcapsules acting as carriers for different payloads, and being dissociated through the formation of aptamer-ligand complexes is described. Specifically, stimuli-responsive anti-adenosine triphosphate (ATP) aptamer-cross-linked DNA-stabilized microcapsules loaded with tetramethylrhodamine-modified dextran (TMR-D), CdSe/ZnS quantum dots (QDs), or microperoxidase-11 (MP-11) are presented. In the presence of ATP as trigger, the microcapsules are dissociated through the formation of aptamer-ATP complexes, resulting in the release of the respective loads. Selective unlocking of the capsules is demonstrated, and CTP, GTP, or TTP do not unlock the pores. The ATP-triggered release of MP-11 from the microcapsules enables the MP-11-catalyzed oxidation of Amplex UltraRed by H2O2 to the fluorescent product resorufin.
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Wei-Ching
WC
Liao
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Chun-Hua
CH
Lu
Fachbereich Physik, Philipps-Universität Marburg , Renthof 7, 35037 Marburg, Germany.
Raimo
R
Hartmann
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Fuan
F
Wang
Institute of Life Science, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Yang Sung
YS
Sohn
Fachbereich Physik, Philipps-Universität Marburg , Renthof 7, 35037 Marburg, Germany.
Wolfgang J
WJ
Parak
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Itamar
I
Willner
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000494:pharmacology; D002214:Capsules / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D045663:Quantum Dots / Q000737:chemistry
D052157:Aptamers, Nucleotide; D002214:Capsules; D010078:Oxazines; C014180:resorufin; D000255:Adenosine Triphosphate; D004247:DNA; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021799:microperoxidase
biocatalyst; controlled release; fluorescence; microperaoxidase-11; quantum dot (QD)