P-selectin gene polymorphism associates with pulmonary hypertension in congenital heart disease.
OBJECTIVE
To investigate the relationship between P-selectin gene polymorphism and congenital heart disease (CHD) with pulmonary hypertension (PAH).


METHODS
58 CHD patients with PAH (PAH-CHD), 43 CHD patients without PAH and 205 healthy subjects were included in this study. The concentration of plasma P-selectin was determined by ELISA kits; the direct sequencing of PCR products was used to analyze the P-selectin genotypes.


RESULTS
The concentration of plasma P-selectin was markedly higher in PAH-CHD patients than that in CHD subjects and controls, while no difference was observed between CHD group and control. A significant difference of P-selectin genotype -825T/C polymorphism was observed between patients with PAH-CHD and healthy subjects (P<0.05). Logistic analysis showed that the subjects with haplotypes A-G and G-G had lower risk of PAH-CHD compared with the ones with haplotype A-A (OR=0.47, 95% CI=0.24-0.92). In the subjects of PAH-CHD and control, plasma P-selectin concentration was higher in subjects with -825TT genotype than the ones with haplotypes T-C and C-C (P<0.05).


CONCLUSIONS
P-selectin probably involves in the development of PAH-CHD. The polymorphism of -825T/C is associated with the risk of PAH-CHD, and may be one of its risk factors.
Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, China.
Xiao-Fei
XF
Li
Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, China.
Chun-Hui
CH
Song
Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, China.
Hong-Zhuan
HZ
Sheng
Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, China.
Dong-Dong
DD
Zhen
Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, China.
Min
M
Pan
Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, China.
Jian-Hua
JH
Zhu
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006330:Heart Defects, Congenital / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006976:Hypertension, Pulmonary / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D019007:P-Selectin / Q000097:blood / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
D005819:Genetic Markers; D019007:P-Selectin; C546937:SELP protein, human
Congenital heart disease; P-selectin; pulmonary hypertension; single-nucleotide polymorphism