Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh).
The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L(-1) FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Liu
L
Hongming
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Lou
L
Xu
Observation and Experimental Station of Saline Land of Coastal Area, Institute of Agricultural Sciences in Coastal Area of Jiangsu, Ministry of Agriculture, Yancheng, China, Observation and Experimental Station of Saline Land of Coastal Area, Institute of Agricultural Sciences in Coastal Area of Jiangsu, Ministry of Agriculture, Yancheng, China.
Ge
G
Zhaojian
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Yang
Y
Fan
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Chen
C
Dingbin
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Zhu
Z
Jianchun
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China. ; Jiangsu Center for GMO Evaluation and Detection, Jiangsu Academy of Agricultural Sciences, Ministry of Agricultrue, Nanjing, China, Key Lab of Agro-Product Safety Risk Evaluation, Jiangsu Center for GMO Evaluation and Detection, Jiangsu Academy of Agricultural Sciences, Ministry of Agricultrue, Nanjing, China.
Xu
X
Jianhong
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Li
L
Shunpeng
Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
Hong
H
Qing
D001711:Biotransformation; D043182:Carboxylesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006540:Herbicides / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010080:Oxazoles / Q000378:metabolism*; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013379:Substrate Specificity; D014908:Triticum / Q000254:growth & development
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006540:Herbicides; D010080:Oxazoles; D011422:Propionates; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; C416661:fenoxaprop ethyl; D043182:Carboxylesterase
FE -hydrolyzing carboxylesterase gene; Rhodococcus ruber JPL-2; fenoxaprop-P-ethyl; microbial degradation