In vitro antibiofilm activity of the freshwater bryozoan Hyalinella punctata: a case study of Pseudomonas aeruginosa PAO1.
The antibiofilm and possible antiquorum sensing effects against the strain Pseudomonas aeruginosa PAO1 of five crude extracts of the freshwater bryozoan Hyalinella punctata (Hancock, 1850) were evaluated in vitro for the first time. H. punctata ethyl acetate extract (HpEtAc) exhibited the highest antibiofilm activity reducing the biofilm formation of P. aeruginosa PAO1 in the range of 80.63-88.13%. While all tested extracts reduced the twitching motility of the aforementioned bacterial strain, HpEtAc showed to be the most effective. Finally, at a concentration of 0.5 MIC, the same extract mostly inhibited the production of pyocyanin by P. aeruginosa PAO1 (71.53%). In comparison both with the positive controls used (streptomycin and ampicillin, 67.13 and 69.77%, respectively), HpEtAc was found to inhibit pyocyanin in a higher extent. An extensive chemical characterisation of this particular extract may result in isolation and identification of novel lead compounds targeting P. aeruginosa, an opportunistic human pathogen.
a Department of Life Sciences, Institute for Multidisciplinary Research - IMSI , University of Belgrade , Belgrade , Serbia.
Boris
B
Pejin
b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research ''Sinisa Stankovic'' , University of Belgrade , Belgrade , Serbia.
Ana
A
Ciric
c Faculty of Sciences, Department of Biology and Ecology , University of Novi Sad , Novi Sad , Serbia.
Ivo
I
Karaman
c Faculty of Sciences, Department of Biology and Ecology , University of Novi Sad , Novi Sad , Serbia.
Mladen
M
Horvatovic
b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research ''Sinisa Stankovic'' , University of Belgrade , Belgrade , Serbia.
Jasmina
J
Glamoclija
b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research ''Sinisa Stankovic'' , University of Belgrade , Belgrade , Serbia.
Milos
M
Nikolic
b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research ''Sinisa Stankovic'' , University of Belgrade , Belgrade , Serbia.
Marina
M
Sokovic
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002013:Bryozoa / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005618:Fresh Water; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D011710:Pyocyanine / Q000096:biosynthesis; D053038:Quorum Sensing / Q000187:drug effects
D000900:Anti-Bacterial Agents; D011710:Pyocyanine
Bryozoa; antibiofilm activity; antiquorum sensing activity; extracts