Differential microRNA expression in the serum of patients with nephrotic syndrome and clinical correlation analysis.
Many different microRNAs existed in nephrotic syndrome patients, and they may be involved in nephrotic syndrome occurrence. In order to further clarify miRNAs expression changes in nephrotic syndrome patients and their correlation with clinical features, this study investigated differential microRNA expression in the peripheral serum of patients with nephrotic syndrome and analyzed the correlation between miRNA with largest overexpression level and clinical features. miRNAs microarray was applied to screen different expressed miRNAs in nephrotic syndrome patients. Real-time PCR was performed to verify miRNA expression level. SPSS software was used to analyze correlation between miRNA expression and clinical features. Compared with healthy subjects, 35 miRNAs overexpressed and 24 miRNAs down-regulated in patients. After real-time PCR verification, 6 miRNAs up-regulated in nephrotic syndrome patients, including hsa-miR-181a, hsa-miR-210, hsa-miR-30a, hsa-miR-942, hsa-miR-192 and hsa-miR-586. miRNA-30a significantly overexpressed in nephrotic syndrome patients and with no difference between genders. miRNA-30a expression level in drug resistant nephrotic syndrome patients was obviously higher than the drug sensitive patients. miRNA-30a up-regulated most significantly in mesangial proliferative glomerulonephritis among different pathology types, while it decreased most obviously in glomerular lesions. miRNA differently expressed in the serum of nephrotic syndrome patients. miRNA-30a could be treated as the molecular marker in predict drug resistance and pathological type of nephrotic syndrome.
Department of Nephrology, Yantaishan Hospital Yantai City, Shandong Province, China.
Jian
J
Teng
Department of Nephrology, Yantaishan Hospital Yantai City, Shandong Province, China.
Fang
F
Sun
Department of Respiratory Medicine, Yantai Yuhuangding Hospital Yantai City 264000, Shandong Province, China.
Peng-Fei
PF
Yu
Department of Labouratory, Yantaishan Hospital Yantai City, Shandong Province, China.
Ji-Xia
JX
Li
Department of Nephrology, Yantaishan Hospital Yantai City, Shandong Province, China.
Dong
D
Yuan
Department of Nephrology, Yantaishan Hospital Yantai City, Shandong Province, China.
Jing
J
Chang
Department of Nephrology, Yantaishan Hospital Yantai City, Shandong Province, China.
Shu-Hua
SH
Lin
D001706:Biopsy; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D005819:Genetic Markers; D006801:Humans; D007668:Kidney / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D009404:Nephrotic Syndrome / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005819:Genetic Markers; D035683:MicroRNAs
Microarray; clinical correlation; miRNA-30a; nephrotic syndrome