Expression of Antioxidant Genes in the Mouse Cochlea and Brain in Salicylate-Induced Tinnitus and Effect of Treatment with Spirulina platensis Water Extract.
Salicylate increased manganese-superoxide dismutase (Mn-SOD) gene expression, but decreased catalase (CAT) gene expression in the cochlea and various brain regions of mice with tinnitus. Spirulinaplatensis water extract reduced salicylate-induced overexpression of the Mn-SOD gene, but increased salicylate-induced downregulation of the CAT gene. With the exception of significantly increased SOD activity in the brainstem and inferior colliculus of the Spirulina group, SOD and CAT enzyme activities did not differ among the three groups. The tinnitus group had higher malondialdehyde (MDA) levels than the control group in the temporal and the frontal lobes. S.platensis water extract reduced salicylate-induced elevations of MDA levels in many brain areas. We proposed that altered expression of antioxidant genes may reflect states of oxidative stress associated with tinnitus.
Department of Otolaryngology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin, Taiwan.
Juen-Haur
JH
Hwang
Nian-Cih
NC
Chang
Jin-Cherng
JC
Chen
Yin-Ching
YC
Chan
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D020156:Salicylic Acid; D053545:Spirulina; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014012:Tinnitus / Q000139:chemically induced / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism
D010936:Plant Extracts; D002374:Catalase; D013482:Superoxide Dismutase; D020156:Salicylic Acid