Directed osteogenic differentiation of mesenchymal stem cell in three-dimensional biodegradable methylcellulose-based scaffolds.
Development of three-dimensional (3D) biodegradable scaffolds that can accelerate mesenchymal stem cell (MSC) osteogenic differentiation is a decisive prerequisite for treatment of damaged skeletal tissue. We report herein the preparation of methylcellulose-based (MC) scaffolds using carbonyldiimidazole as cross-linking agent to produce substrates with specific cross-linking density and porous structure, as well as their applications for directing hMSC toward osteoblasts. The mechanical properties of the scaffolds were controlled by cross-linking density. Human MSCs (hMSCs) seeded on the MC scaffolds have penetrated into the pores, and showed high viability (>80%) as revealed by WST assay and Live/Dead assay. Moreover, the results of differentiation experiments indicated that hMSCs cultured on MC substrates displayed high level of osteogenic differentiation marker expression, alkaline phosphatase activity and osteocalcin secretion, suggesting that the MC scaffolds can direct hMSC differentiation toward osteoblasts without inducer treatment and cross-linking density of MC scaffolds have stimulatory effect on inducing differentiation. The 3D MC scaffolds could be applicable as promising scaffolds for bone tissue repair.
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou 215123, China.
He
H
Shen
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou 215123, China.
Yufei
Y
Ma
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou 215123, China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Yu
Y
Luo
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou 215123, China.
Xiaoyun
X
Liu
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou 215123, China. Electronic address: zjzhang2007@sinano.ac.cn.
Zhijun
Z
Zhang
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou 215123, China; Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30, Gaotanyan Road, Chongqing 400038, China. Electronic address: jwdai@genetics.ac.cn.
Jianwu
J
Dai
D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D003432:Cross-Linking Reagents; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D008747:Methylcellulose / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
D003432:Cross-Linking Reagents; D015675:Osteocalcin; D008747:Methylcellulose; D000469:Alkaline Phosphatase
Cross-linking density; Mesenchymal stem cell; Methylcellulose; Osteogenic differentiation; Three-dimensional scaffold