DNA-stabilized bimetallic nanozyme and its application on colorimetric assay of biothiols.
This paper reports a facile synthesis of bimetallic AuxPty nanozyme using C-rich oligonucleotide as the nucleation template, with high peroxidase-like activity and thiophilicity associated with the ratio of x:y. The efficient Au2Pt1 nanozyme possesses highly peroxidase mimicking activity with the Km value of 0.088 mM toward 3,3',5,5'-tetramethylbenzidine and 196 mM toward hydrogen peroxide. It is the first report to explore colorimetric assays for biothiols with high sensitivity and selectivity against non-thiol-containing amino acids, on the basis of thiol-induced inhibition of peroxidase-like activities of metal nanoparticles. By using Au2Pt1 nanozyme, the limit of detection (LOD) is 3.5 nM for cysteine and 1.6 nM for homocysteine. This DNA-stabilized bimetallic AuxPty nanozyme shows a promising application in quantitative detection of biothiols in biological fluids.
Department of Chemistry and Biology, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
Yanhua
Y
Sun
Department of Chemistry and Biology, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
Jun
J
Wang
Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China.
Wei
W
Li
Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China.
Jinli
J
Zhang
Department of Chemistry and Biology, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
Yaodan
Y
Zhang
Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: fuyan@tju.edu.cn.
Yan
Y
Fu
D000497:Alloys / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005079:Excipients / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009195:Peroxidase / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000032:analysis*
D000497:Alloys; D005079:Excipients; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; D009195:Peroxidase
DNA; Gold; Peroxidase; Platinum; Thiophilicity