Different doses of partial liver irradiation promotes hepatic regeneration in rat.
The aim of this study is to investigate whether partial liver irradiation promotes hepatic regeneration in rat. Left-half liver of rat was irradiated to 10 Gy, and the Right-half to 0, 5, 10 and 15 Gy, respectively. Then, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were evaluated on 0 day, 15-day, 30-day, 45-day and 60-day after liver irradiation. Next, the serum HGF, NF-κB and TGF-β1 levels were also analyzed on 60-day after liver irradiation. Lastly, the cyclinD1 protein expression was appraised by western blots on 60-day after liver irradiation. ALT, AST and ALP levels were reduced compared with that of controls. The serum HGF, NF-κB and TGF-β1 levels, and the cyclinD1 protein expression in liver irradiation group were increased compared with that of controls group. However, hepatic regeneration of higher dose-irradiated cirrhotic liver was triggered a more enhanced regeneration, compared with that of higher doses group. In summary, these results suggest that different doses of partial liver irradiation promotes hepatic regeneration in rat.
Department of Oncology, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Ying
Y
Liu
Medical Imaging Center, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Changzheng
C
Shi
Medical Imaging Center, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Meng
M
Cui
Department of Oncology, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Zhenhua
Z
Yang
Department of Pathology, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Danhui
D
Gan
Department of Oncology, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Yiming
Y
Wang
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D049109:Cell Proliferation / Q000528:radiation effects; D019938:Cyclin D1 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008115:Liver Regeneration / Q000528:radiation effects*; D008297:Male; D023421:Models, Animal; D016328:NF-kappa B / Q000097:blood; D017208:Rats, Wistar; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000097:blood
D015415:Biomarkers; C530106:Ccnd1 protein, rat; D016328:NF-kappa B; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D019938:Cyclin D1; D017228:Hepatocyte Growth Factor; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase
HGF; Liver irradiation; NF-κB; TGF-β1; hepatic regeneration