Magnetic field dependence of spatial frequency encoding NMR as probed on an oligosaccharide.
The magnetic field dependence of spatial frequency encoding NMR techniques is addressed through a detailed analysis of (1)H NMR spectra acquired under spatial frequency encoding on an oligomeric saccharide sample. In particular, the influence of the strength of the static magnetic field on spectral and spatial resolutions that are key features of this method is investigated. For this purpose, we report the acquisition of correlation experiments implementing broadband homodecoupling or J-edited spin evolutions, and we discuss the resolution enhancements that are provided by these techniques at two different magnetic fields. We show that performing these experiments at higher field improves the performance of high resolution NMR techniques based on a spatial frequency encoding. The significant resolution enhancements observed on the correlation spectra acquired at very high field make them valuable analytical tools that are suitable for the assignment of (1)H chemical shifts and scalar couplings in molecules with highly crowded spectrum such as carbohydrates.
Equipe de RMN en milieu orienté, ICMMO, UMR 8182 (CNRS-UPS), Université Paris-Sud, Orsay cedex, 91405, France.
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Pitoux
Equipe Méthodologies, Synthèses et Molécules Thérapeutiques, ICMMO, UMR 8182 (CNRS-UPS), LabEx LERMIT, Université Paris-Sud, Orsay Cedex, 91405, France.|Unité de Chimie des Biomolécules, Institut Pasteur, UMR CNRS 3523, Paris cedex, 75724, France.
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Hu
Equipe de RMN en milieu orienté, ICMMO, UMR 8182 (CNRS-UPS), Université Paris-Sud, Orsay cedex, 91405, France.
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Plainchont
Equipe de RMN en milieu orienté, ICMMO, UMR 8182 (CNRS-UPS), Université Paris-Sud, Orsay cedex, 91405, France.
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Merlet
Equipe de RMN en milieu orienté, ICMMO, UMR 8182 (CNRS-UPS), Université Paris-Sud, Orsay cedex, 91405, France.
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Farjon
Equipe Méthodologies, Synthèses et Molécules Thérapeutiques, ICMMO, UMR 8182 (CNRS-UPS), LabEx LERMIT, Université Paris-Sud, Orsay Cedex, 91405, France.
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Bonnaffé
Equipe de RMN en milieu orienté, ICMMO, UMR 8182 (CNRS-UPS), Université Paris-Sud, Orsay cedex, 91405, France.
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Giraud
D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*
D009844:Oligosaccharides
1H; J-edited spectroscopy; NMR; broadband homodecoupled spectroscopy; broadband homonuclear decoupling; gradient encoded selective refocusing; oligosaccharide; spatial frequency encoding; very high field