Effects of ozone and photo-activated disinfection against Enterococcus faecalis biofilms in vitro.
OBJECTIVE
The purpose of this study was to compare the antibacterial effects of gaseous ozone (O3) and photo-activated disinfection (PAD) methods against Enterococcus faecalis (E. faecalis) biofilms.


METHODS
Sixty-five human mandibular premolars with straight root canals were selected. After root canal preparation, the samples were sterilized and placed into eppendorf tubes with 1 mL brain heart infusion broth containing 1.5×10(8) colony-forming units (CFUs)/mL of E. faecalis. The contaminated samples were then divided into four groups (n=15) according to the disinfection method used: Group 1, Saline (positive control); Group 2, NaOCl (negative control); Group 3, Gaseous O3; and Group 4, PAD. Three non-contaminated teeth were used to control the infection and sterilization process. The CFUs were counted and the data were analyzed statistically.


RESULTS
There was a statistically significant difference between the experimental and control groups (P<0.05). The saline group had the highest number of remaining microorganisms. Complete sterilization was achieved in the 2.5% NaOCl group. There were no statistically differences between PAD and gaseous O3 (P>0.05).


CONCLUSIONS
Both PAD and gaseous O3 have a significant antibacterial effect on infected root canals. However, 2.5% NaOCl was superior in terms of its antimicrobial abilities compared with the other disinfection procedures.
Department of Endodontics, Faculty of Dentistry, Akdeniz University, Konyaalti, Antalya, 07070, Turkey.
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Dinçer
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Kuştarcı
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Dinç
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Demirbuga
D018441:Biofilms / Q000187:drug effects*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D006801:Humans; D010126:Ozone / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods*; D012973:Sodium Hypochlorite / Q000494:pharmacology
D012388:Root Canal Irrigants; D010126:Ozone; D012973:Sodium Hypochlorite