In Vitro Cell Culture Models of Hepatic Steatosis.
The liver is the systemic hub of lipid metabolism. The excessive accumulation of lipids in hepatocytes, steatosis, is a major clinical concern, whose progressive forms lead to end-stage liver disease. Currently, animal studies are the gold standard in toxicological risk assessment. Fueled by an integration of modern omics technologies, in silico models and in vitro system optimization, a new paradigm in the basis for toxicological risk assessment is emerging away from the use of animals. In recent years, in vitro assays have been developed for the early screening of the steatogenic potential of compounds. The present chapter describes an assay for the intracellular detection of lipids, a high-content screen for the distinction between steatosis and phospholipidosis, a multiparametric high-content screen for steatogenic potential and a liver X receptor reporter cell line.
Alexander Grass Center for Bioengineering, Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, 3-512 Silberman Institute of Life Sciences, Jerusalem, 9190401, Israel.
Gahl
G
Levy
Merav
M
Cohen
Yaakov
Y
Nahmias
D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D005234:Fatty Liver / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism
D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D010078:Oxazines; D010743:Phospholipids; C044808:nile red