Effects of chronic exposure to 950 MHz ultra-high-frequency electromagnetic radiation on reactive oxygen species metabolism in the right and left cerebral cortex of young rats of different ages.
OBJECTIVE
To assess the effect of 950 MHz ultra-high-frequency electromagnetic radiation (UHF-EMR) on biomarkers of oxidative damage to DNA, proteins and lipids in the left cerebral cortex (LCC) and right cerebral cortex (RCC) of neonate and 6-day-old rats.


METHODS
Twelve rats were equally divided into two groups as controls (CR) and exposed (ER), for each age (0 and 6 days). The LCC and RCC were examined in ER and CR after exposure. Radiation exposure lasted 30 min per day for up to 27 days (throughout pregnancy and 6 days postnatal). The specific absorption rate ranged from 1.32-1.14 W/kg. The damage to lipids, proteins and DNA was verified by thiobarbituric acid reactive substances, carbonylated proteins (CP) and comets, respectively. The concentration of glucose in the peripheral blood of the rats was measured by the Accu-Chek Active Kit due to increased CP in RCC.


RESULTS
In neonates, no modification of the biomarkers tested was detected. On the other hand, there was an increase in the levels of CP in the RCC of the 6-day-old ER. Interestingly, the concentration of blood glucose was decreased in this group.


CONCLUSIONS
Our results indicate that there is no genotoxicity and oxidative stress in neonates and 6 days rats. However, the RCC had the highest concentration of CP that do not seem to be a consequence of oxidative stress. This study is the first to demonstrate the use of UHF-EMR causes different damage responses to proteins in the LCC and RCC.
a Biotechnology Center, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.|b Department of Biophysics , Institute of Biosciences, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.|c Military School of Porto Alegre, Brazilian Army , Porto Alegre , Brazil.
Orlando V
OV
Furtado-Filho
a Biotechnology Center, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.|b Department of Biophysics , Institute of Biosciences, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
Juliana B
JB
Borba
d Toxicological Genetics Laboratory , Lutheran University of Brazil (ULBRA) , Canoas , RS , Brazil.
Tatiana
T
Maraschin
b Department of Biophysics , Institute of Biosciences, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
Larissa M
LM
Souza
a Biotechnology Center, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.|b Department of Biophysics , Institute of Biosciences, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
João A P
JA
Henriques
e Department of Biochemistry , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
José C F
JC
Moreira
b Department of Biophysics , Institute of Biosciences, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.|f Laboratory of Genetic Toxicology , Federal University of Health Sciences of Porto Alegre (UFCSPA) , Porto Alegre , Brazil.
Jenifer
J
Saffi
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism* / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields; D005260:Female; D008297:Male; D008872:Microwaves; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology / Q000528:radiation effects; D011829:Radiation Dosage; D000069079:Radiation Exposure; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D017382:Reactive Oxygen Species
AGE; Cerebral cortex; UHF-EMR; oxidative damage