Protective role of edaravone against cisplatin-induced ototoxicity in an auditory cell line.
Edaravone is a neuroprotective agent with a potent free radical scavenging and antioxidant actions. In the present study we investigated the influence of edaravone on cisplatin ototoxicity in auditory cells. Cell viability was determined using a 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide cell proliferation assay. Oxidative stress and apoptosis were assessed by reactive oxygen species (ROS) measurement, Hoechst 33258 staining, caspase-3 activity assay, and immunoblotting of PARP. Pretreatment with 100 μM of edaravone prior to application of 15 μM of cisplatin increased cell viability after 48 h of incubation in HEI-OC1 cells (from 51.9% to 64. 6% viability) and also, attenuated the cisplatin-induced increase in reactive oxygen species (ROS) (from 2.3 fold to 1.9 fold). Edaravone also decreased the activation of caspase-3 and reduced levels of cleaved poly-ADP-ribose polymerase (PARP). We propose that edaravone protects against cisplatin-induced ototoxicity by preventing apoptosis, and limiting ROS production in HEI-OC1 cells.
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Anam-Dong 5-Ga 126-1, Sungbuk-Gu, Seoul, South Korea.
Gi Jung
GJ
Im
Department of Otolaryngology-Head and Neck Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 948-1, Daerim 1-dong, Yeongdeunpo-gu, Seoul, South Korea. Electronic address: brune7708@gmail.com.
Jiwon
J
Chang
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Anam-Dong 5-Ga 126-1, Sungbuk-Gu, Seoul, South Korea.
Sehee
S
Lee
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Anam-Dong 5-Ga 126-1, Sungbuk-Gu, Seoul, South Korea.
June
J
Choi
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Anam-Dong 5-Ga 126-1, Sungbuk-Gu, Seoul, South Korea.
Hak Hyun
HH
Jung
Department of Otolaryngology-Head and Neck Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 948-1, Daerim 1-dong, Yeongdeunpo-gu, Seoul, South Korea.
Hyung Min
HM
Lee
Department of Otolaryngology-Head and Neck Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 948-1, Daerim 1-dong, Yeongdeunpo-gu, Seoul, South Korea.
Sung Hoon
SH
Ryu
Department of Otolaryngology-Head and Neck Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 948-1, Daerim 1-dong, Yeongdeunpo-gu, Seoul, South Korea.
Su Kyoung
SK
Park
Department of Otolaryngology-Head and Neck Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 948-1, Daerim 1-dong, Yeongdeunpo-gu, Seoul, South Korea.
Jin Hwan
JH
Kim
Department of Otolaryngology-Head and Neck Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 948-1, Daerim 1-dong, Yeongdeunpo-gu, Seoul, South Korea.
Hyung-Jong
HJ
Kim
D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D000975:Antioxidants / Q000627:therapeutic use; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D002945:Cisplatin / Q000009:adverse effects*; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use; D006198:Hair Cells, Auditory / Q000502:physiology; D006309:Hearing / Q000187:drug effects*; D034381:Hearing Loss / Q000139:chemically induced*; D015151:Immunoblotting; D051379:Mice; D008856:Microscopy, Fluorescence; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D000970:Antineoplastic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C505409:Casp3 protein, mouse; D053148:Caspase 3; D002945:Cisplatin; D000077553:Edaravone; D000983:Antipyrine
Apoptosis; Cell culture; Cisplatin; Edaravone; Ototoxicity