MicroRNA-1908 functions as a glioblastoma oncogene by suppressing PTEN tumor suppressor pathway.
BACKGROUND
We aimed to investigate whether miRNA-1908 is an oncogene in human glioblastoma and find the possible mechanism of miR-1908.


METHODS
We investigated the growth potentials of miRNA-1908-overexpressing SW-1783 cells in vitro and in vivo. In order to identify the target molecule of miRNA-1908, a luciferase reporter assay was performed, and the corresponding downstream signaling pathway was examined using immunohistochemistry of human glioblastoma tissues. We also investigated the miRNA-1908 expression in 34 patients according to the postoperative risk of recurrence.


RESULTS
The overexpression of miRNA-1908 significantly promoted anchorage-independent growth in vitro and significantly increased the tumor forming potential in vivo. MiRNA-1908 significantly suppressed the luciferase activity of mRNA combined with the PTEN 3'-UTR. Furthermore, the expression levels of miRNA-1908 were significantly increased in the patients with a high risk of recurrence compared to that observed in the low-risk patients, and this higher expression correlated with a poor survival.


CONCLUSIONS
miRNA-1908 functions as an oncogene in glioblastoma by repressing the PTEN pathway. MiR-1908 is a potential new molecular marker for predicting the risk of recurrence and prognosis of glioblastoma.
Department of Neurosurgery, Guilin Medical University, Affiliated Hospital, Guilin, 541001, Guangxi, People's Republic of China.
Xuewei
X
Xia
Laboratory of Medical neurobiology, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
Yong
Y
Li
Department of Neurosurgery, Guilin Medical University, Affiliated Hospital, Guilin, 541001, Guangxi, People's Republic of China.
Wenbo
W
Wang
Department of Pathology and Physiopathology, Guilin Medical University, Guilin, 541004, Guangxi, People's Republic of China.
Fang
F
Tang
Laboratory of Medical neurobiology, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
Jie
J
Tan
Laboratory of Medical neurobiology, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
Liyuan
L
Sun
Laboratory of Medical neurobiology, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
Qinghua
Q
Li
Laboratory of Medical neurobiology, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
Li
L
Sun
Department of Hepatobiliary Surgery, Guilin Medical University, Affiliated Hospital, Guilin, 541001, Guangxi, People's Republic of China. tangbocancer@163.com.|Laboratory of Liver Injury and Repair Molecular Medicine, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. tangbocancer@163.com.
Bo
B
Tang
Department of Hepatobiliary Surgery, Guilin Medical University, Affiliated Hospital, Guilin, 541001, Guangxi, People's Republic of China. hsqcancer@163.com.|Laboratory of Liver Injury and Repair Molecular Medicine, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. hsqcancer@163.com.
Songqing
S
He
D000328:Adult; D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000628:therapy; D064593:Heterografts; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009857:Oncogenes / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
C577645:MIRN1908 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; D051059:PTEN Phosphohydrolase