A Comparative Analysis of Antimicrobial Property of Wine and Ozone with Calcium Hydroxide and Chlorhexidine.
BACKGROUND
The antibacterial properties of wine and ozone have been established but their antibacterial efficacies against endodontic pathogens are yet to be ascertained.


OBJECTIVE
The purpose of this study is to comparatively evaluate the antibacterial property of ozonated water, white wine (14%) and de-alcoholised white wine.


METHODS
S.mutans and E.faecalis were subcultured and inoculated in a nutrient broth for 24 hours. The following groups were formulated: Group 1A:2% Chlorhexidine (Control group); Group 1B:White wine; Group 1C:Dealcoholised white wine; Group 1D:Ozonated water; Group 2A: Ca(OH)2 + Chlorhexidine (Control group); Group 2B: White wine + Ca(OH)2; Group 2C:De-alcoholised White wine + Ca(OH)2 + chlorhexidine; Group 2D:White wine + Ca(OH)2 + chlorhexidine and group 2E: Dealcoholised white wine + Ca(OH)2 + chlorhexidine. The samples were allowed to diffuse into the culture medium for two hours, later the S. mutans were streaked on to the blood agar medium and the E. faecalis were streaked on to the Muller Hilton agar medium and incubated for 48 hours at 370C the zone of inhibition was measured after 48 hours.


RESULTS
There was no growth of microorganisms seen with ozonated water. Chlorhexidine showed large zone of inhibition compared to the other groups. White wine has better antimicrobial property than de-alcoholised white wine, but when mixed with calcium hydroxide the dealcoholised white wine has better action against the microorganisms.


CONCLUSIONS
Ozonated water has the best antibacterial property and the antibacterial action of Calcium hydroxide is enhanced when it is mixed with de-alcoholised white wine.
Senior Lecturer, Department of Conservative Dentistry & Endodontics, Madha Dental College , Chennai, India .
Shenbaga Kuttalam
SK
Anand
Professor, Department of Conservative Dentistry & Endodontics, Madha Dental College & Hospital , Chennai, India .
A V Rajesh
AV
Ebenezar
Former Post-Graduate Student, Department of Prosthodontics, Rajah Muthaiah Dental College & Hospital , Chidambaram, Tamilnadu, India .
Nirupa
N
Anand
Reader, Department of Preventive and Community Dentistry, Thai Mookambigai Dental College , Chennai, India .
A Vinita
AV
Mary
Lecturer, Department of Conservative Dentistry & Endodontics, Tagore Dental Colleg & Hospital , Chennai, India .
Bejoy
B
Mony
Antimicrobial efficacy; Enterocococcus feacalis; Reactive oxygen species; Streptococcus mutans