Testicular myxosporidiasis and ultrastructural characteristics of Myxobolus bufonis (Myxobolidae) infecting the Egyptian toad Bufo regularis (Bufonidae). A light and electron microscopic study.
The phylum Myxozoa comprises more than 2180 species, almost all of which are considered to be obligate parasites of aquatic fishes and amphibians. They are dangerous pathogens responsible for severe economic losses. From March to September 2014, 40 adult male Bufo regularis (Bufonidae) captured from different areas at Giza province, Egypt, were surveyed for myxosporean parasitic infection. Of these, 22 (55%) were infected by histozoic plasmodia, which produced spores after rupture belonging to Myxosporidia. The present investigation introduced a new data for the recorded parasite observed by light and transmission electron microscopy. The infection was diagnosed as large clusters of macroscopic plasmodia embedded in the testicular tissue causing distortion at the site of infection. The host reaction was manifested by the encapsulation of the plasmodia with a thick layer of connective tissue. Plasmodia were whitish in color, elliptical to ovoid in shape measuring 0.54 ± 0.2 (0.34-0.63) mm in diameter. The spores were subspherical, reaching 7.1 ± 0.2 (6.2-8.4) μm in length and 6.3 ± 0.2 (5.8-7.0) μm in width with two equal-sized polar capsules regularly arranged at the anterior pole of each spore. They were 3.4 ± 0.2 (3.0-4.2) μm in length and 1.9 ± 0.2 (1.6-2.4) in width with 6-8 turns of polar filaments. Ultrastructural analysis showed that the plasmodia were surrounded by a plasma membrane with numerous projections and pinocytotic channels extended toward the host cell. The generative cells and the different developmental stages were arranged at the periphery of the plasmodia while immature and mature spores were centrally arranged. Sporogenesis, capsulogenesis, valvogenesis, and spore maturation of the present parasite were also described.
Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
Kareem
K
Morsy
Electron Microscope Unit, Diabetes Research Institute, Düsseldorf University, Düsseldorf, Germany. msemmlereg@yahoo.de.
Margit
M
Semmler
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Ebtsam
E
Al-Olayan
Department of Parasitology, Düsseldorf University, Düsseldorf, Germany.
Heinz
H
Mehlhorn
D000818:Animals; D002025:Bufonidae / Q000469:parasitology*; D004534:Egypt; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D055766:Myxobolus / Q000648:ultrastructure*; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D013170:Spores / Q000648:ultrastructure
Bufo regularis; Myxobolus bufonis; Myxosporidiasis; Ultrastructure