Diagnostic value of protein chips constructed by lung-cancer-associated markers selected by the T7 phage display library.
BACKGROUND
Traditional diagnostic technology with tumor biomarkers is inefficient, expensive and requires a large number of serum samples. The purpose of this study was to construct human lung cancer protein chips with new lung cancer biomarkers screened by the T7-phage display library, and improve the early diagnosis rate of lung cancer.


METHODS
A T7-phage cDNA display library was constructed of fresh samples from 30 lung cancer patients. With biopanning and high-throughput screening, we gained the immunogenic phage clones from the cDNA library. The insert of selected phage was blasted at GeneBank for alignment to find the exact or the most similar known genes. Protein chips were then constructed and used to assay their expression level in lung cancer serum from 217 cases of lung cancer groups:80 cases of benign lung disease and 220 healthy controls.


RESULTS
After four rounds of Biopanning and two rounds of enzyme-linked immunosorbent assay, 12 phage monoclonal samples were selected from 2880 phage monoclonal samples. After blasting at GeneBank, six similar genes were used to construct diagnostic protein chips. The protein chips were then used to assay expression level in lung cancer serum. The expression level of six genes in lung cancer groups was significantly higher than those in the other two groups (P < 0.05).


CONCLUSIONS
In this study, we successfully constructed diagnostic protein chips with biomarkers selected from the lung cancer T7-phage cDNA library, which can be used for the early screening of lung cancer patients.
Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, China.
Hong-Mei
HM
Li
Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, China.
Kang
K
Guo
Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, China.
Zhuang
Z
Yu
Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, China.
Rui
R
Feng
Cancer Center, The Affiliated Hospital of Qingdao University Qingdao, China.
Ping
P
Xu
Biomarkers; T7 phage; lung neoplasms; protein microarray