Optimal design for studying mucoadhesive polymers interaction with gastric mucin using a quartz crystal microbalance with dissipation (QCM-D): Comparison of two different mucin origins.
The objective of this present study was to develop an efficient and simple method, based on the use of a quartz crystal microbalance with dissipation (QCM-D), to evaluate the mucoadhesive characteristics of cationic polymers; chitosan, thiolated chitosan (chitosan-SH), and polyallylamine hydrochloride (PAH), and anionic polymers; hyaluronic acid (HA) and thiolated hyaluronic acid (HA-SH). The experiments were carried out at pH 4 to assess the interaction between mucoadhesive polymers and a mucin-coated gold surface. A key point in the QCM-D protocol development was to evaluate two sources of mucin: native porcine gastric mucin (NPGM) and commercially available porcine gastric mucin (CPGM). QCM-D has shown its potential as a highly sensitive technique that provides information about the interaction of mucoadhesive polymers with gastric mucin. The technique would allow the classification of these polymers in order to further assess their application as base materials for nanocarriers, designed to interact with the mucosal barrier which represents a stumbling block for drug adsorption.
Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona 08017, Spain; Sagetis-Biotech, Barcelona, Spain.
Sejin
S
Oh
The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Matthew
M
Wilcox
The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Jeffrey P
JP
Pearson
Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona 08017, Spain; Sagetis-Biotech, Barcelona, Spain. Electronic address: salvador.borros@iqs.edu.
Salvador
S
Borrós
D000003:Abattoirs; D000268:Adhesiveness; D000327:Adsorption; D000818:Animals; D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D055119:Elastic Modulus; D005753:Gastric Mucosa / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009093:Mucus / Q000737:chemistry / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism*; D058525:Quartz Crystal Microbalance Techniques; D033081:Shear Strength; D013438:Sulfhydryl Compounds / Q000737:chemistry; D034421:Sus scrofa; D014783:Viscosity
D011073:Polyamines; D013438:Sulfhydryl Compounds; C063994:polyallylamine; D006820:Hyaluronic Acid; D048271:Chitosan
Adsorption; CPGM; Interaction; Mucin; Mucoadhesive polymers; NPGM; QCM-D