Association analysis of the IL-1 gene cluster polymorphisms with aggressive and chronic periodontitis in the Algerian population.
OBJECTIVE
There is strong evidence that genetic as well as environmental factors affect the development of periodontitis. Various studies suggest that genetic polymorphisms of the interleukin-1 (IL-1) genes are associated with an increased risk of developing the pathogenesis. The aim of the present study was to investigate the possible relationship between two polymorphisms of IL-1 gene cluster IL-1B (C+3954T) (rs1143634) and IL-1A (C-889T) (rs1800587) SNPs and the aggressive and chronic periodontitis risk in a case control study in Algerian population.


METHODS
279 subjects were recruited and received a periodontal examination: 128 healthy controls and 151 cases. From cases, 91 patients were having a chronic disease whereas 60 subjects with aggressive form. All these subjects were genotyped for IL-1A (C-889T) and IL-1B (C+3954T) polymorphisms using TaqMan real time PCR technology. Frequencies of IL-1 alleles, genotypes and the haplotypes were also examined.


RESULTS
Significant differences were found in the carriage rate of both minor alleles of the IL-1A (C-889T) and IL-1B (C+3954T) polymorphisms of aggressive periodontitis cases compared with healthy controls (OR [95%CI]=1.61 [1.03-2.49], p=0.03), (OR [95%CI]=1.69 [1.09-2.63], p=0.01), respectively. The result did not reach significance with the chronic form.


CONCLUSIONS
The studied polymorphisms of the IL-1 genes appear to be associated with susceptibility to aggressive periodontitis (AgP) in the Algerian population.
Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran-Mohamed BOUDIAF-USTOMB-, BP 1505 El M'naouer, 31036 Oran, Algeria. Electronic address: boukortt.kawther@gmail.com.
Kawther Nourelhouda
KN
Boukortt
Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université d'Oran, BP 1524, El M'Naouar, 31000 Oran, Algeria.
Nadjia
N
Saidi-Ouahrani
Service de parodontologie Département de chirurgie dentaire CHU de Sétif, Oran, Algeria.
Boubaker
B
Boukerzaza
Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran-Mohamed BOUDIAF-USTOMB-, BP 1505 El M'naouer, 31036 Oran, Algeria.
Hadjira
H
Ouhaibi-Djellouli
Service de parodontologie Département de chirurgie dentaire EPSP d'Oran, Oran, Algeria.
Khalida
K
Hachmaoui
Service de parodontologie Département de chirurgie dentaire CHU de Sidi Belabess, Oran, Algeria.
Fatima Zohra
FZ
Benaissa
Service de parodontologie Département de chirurgie dentaire CHU de Tlemcen, Oran, Algeria.
Leila
L
Taleb
Service de parodontologie Département de chirurgie dentaire CHU de Sétif, Oran, Algeria.
Hayet
H
Drabla-Ouahrani
Faculté de médecine d'Oran, université d'Oran, Oran, Algeria.
Tahria
T
Deba
Service de parodontologie Département de chirurgie dentaire EPSP d'Oran, Oran, Algeria.
Sid Ahmed
SA
Ouledhamou
Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran-Mohamed BOUDIAF-USTOMB-, BP 1505 El M'naouer, 31036 Oran, Algeria.
Nadhera
N
Mehtar
Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran-Mohamed BOUDIAF-USTOMB-, BP 1505 El M'naouer, 31036 Oran, Algeria.
Abdellah
A
Boudjema
D000328:Adult; D010520:Aggressive Periodontitis; D000462:Algeria / Q000453:epidemiology; D000483:Alleles; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000453:epidemiology / Q000235:genetics*; D003774:Dental Plaque Index; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D020641:Polymorphism, Single Nucleotide
C508584:IL1A protein, human; C508585:IL1B protein, human; D053582:Interleukin-1alpha; D053583:Interleukin-1beta
Aggressive periodontitis; Algerian population; Chronic periodontitis; Gene; Interleukin-1; Polymorphism