[Sodium houttuyfonate inhibits virulence related motility of Pseudomonas aeruginosa].
Sodium houttuyfonate (SH) is a derivative of effective component of a Chinese material medica, Houttuynia cordata, which is applied in anti-infection of microorganism. But, the antimicrobial mechanisms of SH still remain unclear. Here, we firstly discovered that SH effectively inhibits the three types of virulence related motility of.Pseudomonas aeruginosa, i.e., swimming, twitching and swarming. The plate assay results showed that the inhibitory action of SH against swimming and twitching in 24 h and swarming in 48 h is dose-dependent; and bacteria nearly lost all of the motile activities under the concentration of 1 x minimum inhibitory concentration (MIC) (512 mg x L(-1) same as azithromycin positive group (1 x MIC, 16 mg x L(-1)). Furthermore, we found that the expression of structural gene flgB and pilG is down-regulated by SH, which implies that inhibitory mechanism of SH against motility of P. aeruginosa may be due to the inhibition of flagella and pili bioformation of P. aeruginosa by SR Therefore, our presented results firstly demonstrate that SH effectively inhibits the motility activities of P. aeruginosa, and suggest that SH could be a promising antipseudomonas agents in clinic.
Da-qiang
DQ
Wu
Wei-feng
WF
Huang
Qiang-jun
QJ
Duan
Hui-juan
HJ
Cheng
Chang-zhong
CZ
Wang
D000473:Alkanes / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D052859:Houttuynia / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D013447:Sulfites / Q000494:pharmacology*; D014774:Virulence / Q000187:drug effects
D000473:Alkanes; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004365:Drugs, Chinese Herbal; C442781:Houttuynia cordata extract; D013447:Sulfites; C473296:sodium houttuyfonate