Development of polymorphic microsatellite loci for conservation genetic studies of the coral reef fish Centropyge bicolor.
A total of 23 novel polymorphic microsatellite marker loci were developed for the angelfish Centropyge bicolor through 454 sequencing, and further tested on two spatially separated populations (90 individuals each) from Kimbe Bay in Papua New Guinea. The mean ± s.e. number of alleles per locus was 14·65 ± 1·05, and mean ± s.e. observed (HO ) and expected (HE ) heterozygosity frequencies were 0·676 ± 0·021 and 0·749 ± 0·018, respectively. The markers reported here constitute the first specific set for this genus and will be useful for future conservation genetic studies in the Indo-Pacific region.
Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955, Kingdom of Saudi Arabia.
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Herrera
Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile, Valdivia, Chile.
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Saenz-Agudelo
Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955, Kingdom of Saudi Arabia.
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Nanninga
Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955, Kingdom of Saudi Arabia.
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Berumen
D000483:Alleles; D000818:Animals; D003247:Conservation of Natural Resources; D057894:Coral Reefs; D005399:Fishes / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats; D010219:Papua New Guinea; D011110:Polymorphism, Genetic
Kimbe Bay; Pomacanthidae; connectivity; genetic markers; population genetics