Energy Status Characteristics of Porcine Oocytes During In Vitro Maturation is Influenced by Their Meiotic Competence.
The characteristics of energy status in porcine oocytes as related to their meiotic competence and in vitro maturation were studied. Cycling pubertal gilts in the early luteal to early follicular phases of the ovarian cycle were used as oocyte donors. The oocytes recovered from medium (MF) or small follicles (SF) were considered meiotically more or less competent, respectively. A half of oocytes from each category was matured by the standard protocol. The oocytes were examined before or after maturation by confocal microscopy, a bioluminescent cell assay and Western blotting. Four experiments, each in triplicate, were performed to assess both SF and MF oocytes in terms of metabolic units formed by mitochondria and lipids, ATP and lipid consumption and lipid droplets with adipose differentiation-related protein (ADRP) expression. The proportion of oocytes with metabolic units, the mean ATP content and the number of lipid droplets per oocyte, and the relative number of lipid droplets with ADRP expression were significantly higher in the MF compared to SF oocytes before maturation. On the other hand, after maturation, there was an increase in the proportion of oocytes with metabolic units and the relative number of lipid droplets with ADRP expression in the SF compared to MF oocytes. In conclusion, specific differences in energy characteristics between porcine oocytes with different meiotic competence were found. Meiotically more competent oocytes are more advanced in terms of energy reserves before maturation, while meiotically less competent oocytes are more active in replenishing energy stores during maturation.
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
I
I
Milakovic
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
M
M
Jeseta
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
S
S
Hanulakova
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
D
D
Knitlova
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
K
K
Hanzalova
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
P
P
Hulinska
Department of Animal Breeding, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
L
L
Machal
Department of Histology and Embryology, Department of Anatomy, Poznan University of Medical Science, Poznan, Poland.
B
B
Kempisty
Institute of Veterinary Sciences, Poznan University of Life Sciences, Poznan, Poland.
P
P
Antosik
Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
M
M
Machatkova
D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D066292:Lipid Droplets / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis; D008540:Meiosis / Q000502:physiology*; D008565:Membrane Proteins / Q000032:analysis; D018613:Microscopy, Confocal; D009865:Oocytes / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000033:anatomy & histology; D000070780:Perilipin-2; D034421:Sus scrofa
D008055:Lipids; D008565:Membrane Proteins; D000070780:Perilipin-2