Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways.
Renal cell carcinoma has become the most common subtype of kidney cancer, and has the highest propensity to manifest as metastatic disease. Because of lack of knowledge in events that correlated with tumor cell migration and invasion, few therapeutic options are available. Therefore, in current study, we explore the anti-tumoral effect of a potential chemopreventive natural product, quercetin, combined with anti-sense oligo gene therapy (inhibiting Snail gene). We found that either one of them had the remarkable effects in suppressing cell proliferation and migration, inducing cell cycle arrest and apoptosis in a ccRCC cell line, Caki-2 cells. The combination of both means provides even strong suppressive effects toward these ccRCC cells. Our study, for the first time, provides the possibility of using a novel treatment for renal cancer, by combining natural product and gene therapy.
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
Fan-Dong
FD
Meng
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
Yan
Y
Li
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
Xin
X
Tian
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
Ping
P
Ma
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
Cheng-Guang
CG
Sui
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
Li-Ye
LY
Fu
Department of Molecular Oncology, Cancer Research Institution, The First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning Province, China.
You-Hong
YH
Jiang
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D000067492:RNAi Therapeutics; D015398:Signal Transduction / Q000187:drug effects; D000071250:Snail Family Transcription Factors; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D011794:Quercetin; C546842:MTOR protein, human; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
AKT/mTOR/ERK1/2; Snail; quercetin; renal cell carcinoma