The platelet-activating factor acetylhydrolase gene derived from Trichoderma harzianum induces maize resistance to Curvularia lunata through the jasmonic acid signaling pathway.
Platelet-activating factor acetylhydrolase (PAF-AH) derived from Trichoderma harzianum was upregulated by the interaction of T. harzianum with maize roots or the foliar pathogen Curvularia lunata. PAF-AH was associated with chitinase and cellulase expressions, but especially with chitinase, because its activity in the KO40 transformant (PAF-AH disruption transformant) was lower, compared with the wild-type strain T28. The result demonstrated that the colonization of maize roots by T. harzianum induced systemic protection of leaves inoculated with C. lunata. Such protection was associated with the expression of inducible jasmonic acid pathway-related genes. Moreover, the data from liquid chromatography-mass spectrometry confirmed that the concentration of jasmonic acid in maize leaves was associated with the expression level of defense-related genes, suggesting that PAF-AH induced resistance to the foliar pathogen. Our findings showed that PAF-AH had an important function in inducing systemic resistance to maize leaf spot pathogen.
a Department of Resource and Environmental Science , School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai , P. R. China.
Chuanjin
C
Yu
Lili
L
Fan
Jinxin
J
Gao
Meng
M
Wang
Qiong
Q
Wu
Jun
J
Tang
Yaqian
Y
Li
Jie
J
Chen
D001203:Ascomycota / Q000187:drug effects* / Q000276:immunology; D002688:Chitinases / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D060467:Disease Resistance / Q000187:drug effects* / Q000235:genetics / Q000276:immunology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D054883:Oxylipins / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology*; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D015398:Signal Transduction; D014242:Trichoderma / Q000737:chemistry*; D003313:Zea mays / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology
D003517:Cyclopentanes; D005656:Fungal Proteins; D054883:Oxylipins; C011006:jasmonic acid; D002688:Chitinases
PAF-AH; T. harzianum; inducible maize resistance; qRT-PCR