Melatonin, quercetin and resveratrol attenuates oxidative hepatocellular injury in streptozotocin-induced diabetic rats.
In this study, effects of melatonin, quercetin and resveratrol on hepatocellular injury in streptozotocin (STZ)-induced experimental diabetes were aimed to be investigated by histological and biochemical methods. Thirty-five male Wistar albino rats were divided into five groups, namely, control, diabetes (STZ 45 mg/kg/single dose/intraperitoneally (ip)), diabetes + melatonin (10 mg/kg/30 days/ip), diabetes + quercetin (25 mg/kg/30 days/ip) and diabetes + resveratrol (10 mg/kg/30 days/ip). Initial and final blood glucose levels and body weights (BWs) were measured. At the end of the experimentation, following routine tissue processing procedure, sections were stained with haematoxylin-eosin (H-E), periodic acid Schiff and Masson's trichrome. Tissue malondialdehyde (MDA) and glutathione (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities were examined. The diabetic rats had significantly higher blood glucose levels than those of control rats (p = 0.0001). Mean BWs of diabetic rats were significantly decreased when compared with the control rats (p = 0.0013). Histopathological alterations including cellular glycogen depletion, congestion, sinusoidal dilatation, inflammation and fibrosis were detected in diabetes group. On the other hand, histopathological changes markedly reduced in all of the treatment groups (p = 0.001). Mean tissue MDA level was increased but mean tissue CAT and SOD activities and GSH levels were decreased in the diabetes group. Melatonin, quercetin and resveratrol administered diabetic rats showed an increase in CAT activities and GSH levels and a decrease in MDA levels (p < 0.05, for all). Melatonin, quercetin and resveratrol administrations markedly reduced hepatocellular injury in STZ-induced experimental diabetes.
Department of Histology and Embryology, Faculty of Medicine, MUGLA SITKI KOÇMAN UNIVERSITY, MUGLA, TURKEY h_elbe@hotmail.com.
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H
Elbe
Department of Histology and Embryology, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
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M
Esrefoglu
Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey.
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N
Vardi
Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey.
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E
Taslidere
Department of Biochemistry, Faculty of Medicine, Inonu University, Malatya, Turkey.
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E
Ozerol
Department of Biochemistry, Faculty of Medicine, Inonu University, Malatya, Turkey.
K
K
Tanbek
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D015415:Biomarkers; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D048909:Diabetes Complications / Q000188:drug therapy* / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D006505:Hepatitis / Q000188:drug therapy* / Q000209:etiology* / Q000473:pathology; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D013267:Stilbenes; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase; D008550:Melatonin; D000077185:Resveratrol
Diabetes; hepatic damage; melatonin; quercetin; resveratrol