Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury.
The kidney is essential for fluid homeostasis, blood pressure regulation and filtration of waste from the body. The fundamental unit of kidney function is the nephron. Mammals are able to repair existing nephrons after injury, but lose the ability to form new nephrons soon after birth. In contrast to mammals, adult fish produce new nephrons (neonephrogenesis) throughout their lives in response to growth requirements or injury. Recently, lhx1a has been shown to mark nephron progenitor cells in the adult zebrafish kidney, however mechanisms controlling the formation of new nephrons after injury remain unknown. Here we show our method for robust and reproducible injury in the adult zebrafish kidney by intraperitoneal (i.p.) injection of gentamicin, which uses a noninvasive visual screening process to select for fish with strong but nonlethal injury. Using this method, we can determine optimal gentamicin dosages for injury and go on to demonstrate the effect of higher temperatures on kidney regeneration in zebrafish.
Nephrology Division, Department of Medicine, Massachusetts General Hospital.
Caramai N
CN
Kamei
Nephrology Division, Department of Medicine, Massachusetts General Hospital; Basic Sciences Division, Fred Hutchinson Cancer Research Center.
Yan
Y
Liu
Nephrology Division, Department of Medicine, Massachusetts General Hospital; Department of Genetics, Harvard Medical School; idrummond@mgh.harvard.edu.
Iain A
IA
Drummond
D058186:Acute Kidney Injury / Q000139:chemically induced*; D000818:Animals; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D009399:Nephrons / Q000187:drug effects / Q000502:physiology; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology*; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology; D015027:Zebrafish / Q000502:physiology*
D005839:Gentamicins