Optical spectroscopic determination of human meniscus composition.
This study investigates the correlation between the composition of human meniscus and its absorption spectrum in the visible (VIS) and near infrared (NIR) spectral range. Meniscus samples (n = 24) were obtained from nonarthritic knees of human cadavers with no history of joint diseases. Specimens (n = 72) were obtained from three distinct sections of the meniscus, namely; anterior, center, posterior. Absorption spectra were acquired from each specimen in the VIS and NIR spectral range (400-1,100 nm). Following spectroscopic probing, the specimens were subjected to biochemical analyses to determine the matrix composition, that is water, hydroxyproline, and uronic acid contents. Multivariate analytical techniques, including principal component analysis (PCA) and partial least squares (PLS) regression, were then used to investigate the correlation between the matrix composition and it spectral response. Our results indicate that the optical absorption of meniscus matrix is related to its composition, and this relationship is optimal in the NIR spectral range (750-1,100 nm). High correlations (R(2) (uronic)  = 86.9%, R(2) (water)  = 83.8%, R(2) (hydroxyproline)  = 81.7%, p < 0.0001) were obtained between the spectral predicted and measured meniscus composition, thus suggesting that spectral data in the NIR range can be utilized for estimating the matrix composition of human meniscus. In conclusion, optical spectroscopy, particularly in the NIR spectral range, is a potential method for evaluating the composition of human meniscus. This presents a promising technique for rapid and nondestructive evaluation of meniscus integrity in real-time during arthroscopic surgery.
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.|Diagnostic Imaging Centre, Kuopio University Hospital, Kuopio, Finland.
Juho
J
Ala-Myllymäki
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.|Diagnostic Imaging Centre, Kuopio University Hospital, Kuopio, Finland.
Juuso T J
JT
Honkanen
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.|Diagnostic Imaging Centre, Kuopio University Hospital, Kuopio, Finland.
Juha
J
Töyräs
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.|Diagnostic Imaging Centre, Kuopio University Hospital, Kuopio, Finland.
Isaac O
IO
Afara
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008592:Menisci, Tibial / Q000737:chemistry*; D008875:Middle Aged; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013057:Spectrum Analysis; D055815:Young Adult
composition; meniscus; near infrared (NIR) spectroscopy; osteoarthritis; visible spectroscopy