Water structure at the interfaces between a zwitterionic self-assembled monolayer/liquid water evaluated by sum-frequency generation spectroscopy.
A silane coupling agent having a zwitterionic end group was covalently bound to a semi-cylindrical fused silica prism for sum-frequency generation (SFG) analyses and to a flat glass for estimating biological affinity. It was found that total intensity of the -derived from water in contact with a positively or negatively charged SAM-modified surface and a bare silica prism. These results indicated that water molecules in the vicinity of the zwitterionic SAM-modified surface are not strongly oriented in comparison with those of lopsidedly charged SAMs and bare silica. Moreover, the zwitterionic SAM surface suppressed non-specific adsorption of bovine serum albumin in contrast to the significant adsorption to lopsidedly charged SAMs and the bare cover glass. On the other hand, fibroblasts gradually adhered to the SAM surfaces and extended regardless of the electrical charge of the SAM, though the number of cells that adhered to the zwitterionic SAM was the smallest. The results strongly suggested that the charge neutralization of a solid material surface is very important for anti-biofouling properties.
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
Kouji
K
Nomura
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
Shiho
S
Mikuni
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan; Frontier Research Core for Life Sciences, University of Toyama, Toyama 930-8555, Japan.
Tadashi
T
Nakaji-Hirabayashi
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
Makoto
M
Gemmei-Ide
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan. Electronic address: kitano@eng.u-toyama.ac.jp.
Hiromi
H
Kitano
International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), and Global Research Center for Environment and Energy Based on Nanomaterials Science (GREEN), Tsukuba 305-0044, Japan.
Hidenori
H
Noguchi
International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), and Global Research Center for Environment and Energy Based on Nanomaterials Science (GREEN), Tsukuba 305-0044, Japan. Electronic address: uoaski.kohei@nims.jp.
Kohei
K
Uosaki
D000327:Adsorption; D000818:Animals; D005347:Fibroblasts; D051379:Mice; D041681:NIH 3T3 Cells; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D012821:Silanes; D014867:Water; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide
Biocompatibility; Orientation of water; Self-assembled monolayer; Sum frequency generation; Zwitterionic SAM