Super-Resolution Single-Molecule Localization Microscopy: Tricks of the Trade.
Application of single-molecule fluorescence detection has led to the development of light microscopy techniques that make it possible to study fluorescent samples at spatial resolutions significantly improved upon the diffraction limit of light. The biological and materials science applications of these "super-resolution" microscopy methods are vast, causing current demand for them to be high. However, implementation, execution, and interpretation of these techniques, particularly involving biological samples, require a broad interdisciplinary skillset, not often found in a single laboratory. Those already used to interdisciplinary work as well as navigating communication and collaboration between more pure forms of physics, chemistry, and biology are well-positioned to spearhead such efforts. In this Perspective, we describe various aspects of single-molecule super-resolution imaging, discussing, in particular, the role that physical chemistry has so far played in its development and establishment. We also highlight a selection of some of the remarkable recent research achievements in this vibrant field.
School of Chemistry, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
Donna R
DR
Whelan
School of Chemistry, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
Toby D M
TD
Bell