Introduction to the diagnosis and classification of monocytic-lineage leukemias by flow cytometry.
Despite diagnostic criteria are currently available for the distinct subtypes of monocytic-lineage neoplasias, a number of partially overlapping features still remain evident, which may hamper their differential diagnosis. An accurate identification and characterization of monocytic cells is of major relevance for the diagnosis and classification of these neoplasias. In this regard, as compared to other conventional techniques, flow cytometry has shown the highest sensitivity for detection of early monocytic commitment of (normal and neoplastic) bone marrow CD34+hematopoietic precursors as well as of monocytic aberrations and maturation blockades, which are frequently associated with clonal myeloid disorders. In the present paper we provide basic principles and criteria for multiparameter flow cytometry identification and characterization of bone marrow monocytic cells that contribute to an improved diagnostic and classification of monocytic lineage-associated acute leukemias in clinical settings, particularly when using the EuroFlow antibody panels. © 2015 International Clinical Cytometry Society.
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Sergio
S
Matarraz
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Julia
J
Almeida
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Juan
J
Flores-Montero
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Quentin
Q
Lécrevisse
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Valentina
V
Guerri
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Antonio
A
López
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Susana
S
Bárrena
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Vincent H J
VHJ
Van Der Velden
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Jeroen G
JG
Te Marvelde
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Jacques J M
JJM
Van Dongen
Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain.
Alberto
A
Orfao
D001854:Bone Marrow Cells / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D003937:Diagnosis, Differential; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D009000:Monocytes / Q000473:pathology*; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology
acute leukemia; flow cytometry; monocytes