Identification of Nocardia Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Nocardia species remains challenging. By identifying 83.1% (64 of 77) and 80% (8 of 10) to the species and complex levels, respectively, and 94.3% (82 of 87) to the genus level, we show that an approach using routine sample preparation, an up-to-date commercial database minimally augmented with custom spectra, and testing at an early stage of growth is promising.
ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
Prasanna D
PD
Khot
ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
Brian A
BA
Bird
ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA University of Utah Department of Pathology, Salt Lake City, Utah, USA.
Robert J
RJ
Durrant
ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA University of Utah Department of Pathology, Salt Lake City, Utah, USA mark.fisher@aruplab.com.
Mark A
MA
Fisher
D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D009615:Nocardia / Q000737:chemistry* / Q000145:classification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*