Evaluation of DNA methyltransferase activity and inhibition via chiroplasmonic assemblies of gold nanoparticles.
Circular dichroism spectroscopy has been explored for detection of methyltransferase activity and inhibition based on DNA-induced chiroplasmonic assemblies of gold nanoparticles and endonuclease HpaII. Good accuracy, precision and sensitivity are obtained in complex matrices such as human serum samples, which is significant for clinical diagnosis and drug development.
Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China. wei_wei98@163.com.
Yuanjian
Y
Liu
Min
M
Wei
Linqun
L
Zhang
Wei
W
Wei
Yuanjian
Y
Zhang
Songqin
S
Liu
D002942:Circular Dichroism / Q000379:methods*; D004247:DNA / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D019003:Deoxyribonuclease HpaII / Q000037:antagonists & inhibitors / Q000097:blood* / Q000378:metabolism*; D019281:Dimerization; D004353:Drug Evaluation, Preclinical / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
D004791:Enzyme Inhibitors; D006046:Gold; D004247:DNA; C081573:DNA modification methylase SssI; D015257:DNA-Cytosine Methylases; D019003:Deoxyribonuclease HpaII