Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae.
Increasing demand for renewable fuels has researchers investigating the feasibility of alternative feedstocks, such as microalgae. Inherent advantages include high potential yield, use of non-arable land and integration with waste streams. The nutrient requirements of a large-scale microalgae production system will require the coupling of cultivation systems with industrial waste resources, such as carbon dioxide from flue gas and nutrients from wastewater. Inorganic contaminants present in these wastes can potentially lead to bioaccumulation in microalgal biomass negatively impact productivity and limiting end use. This study focuses on the experimental evaluation of the impact and the fate of 14 inorganic contaminants (As, Cd, Co, Cr, Cu, Hg, Mn, Ni, Pb, Sb, Se, Sn, V and Zn) on Nannochloropsis salina growth. Microalgae were cultivated in photobioreactors illuminated at 984 µmol m(-2) sec(-1) and maintained at pH 7 in a growth media polluted with inorganic contaminants at levels expected based on the composition found in commercial coal flue gas systems. Contaminants present in the biomass and the medium at the end of a 7 day growth period were analytically quantified through cold vapor atomic absorption spectrometry for Hg and through inductively coupled plasma mass spectrometry for As, Cd, Co, Cr, Cu, Mn, Ni, Pb, Sb, Se, Sn, V and Zn. Results show N. salina is a sensitive strain to the multi-metal environment with a statistical decrease in biomass yieldwith the introduction of these contaminants. The techniques presented here are adequate for quantifying algal growth and determining the fate of inorganic contaminants.
Mechanical and Aerospace Engineering, Utah State University.
Katerine
K
Napan
Mechanical and Aerospace Engineering, Utah State University.
Derek
D
Hess
Mechanical and Aerospace Engineering, Utah State University.
Brian
B
McNeil
Mechanical and Aerospace Engineering, Utah State University; Jason.Quinn@usu.edu.
Jason C
JC
Quinn
D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002245:Carbon Dioxide / Q000032:analysis; D005740:Gases / Q000032:analysis; D007220:Industrial Waste; D019216:Metals, Heavy / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D058086:Microalgae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D058009:Stramenopiles / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry
D002245:Carbon Dioxide; D005740:Gases; D007220:Industrial Waste; D019216:Metals, Heavy; D062065:Wastewater