Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload.
Lead (Pb) poisoning has always been a serious health concern, as it permanently damages the central nervous system. Chronic Pb accumulation in the human body disturbs oligodendrocytes (OLs) differentiation, resulting in dysmyelination, but the molecular mechanism remains unknown. In this study, Pb at 1 μM inhibits OLs precursor cells (OPCs) differentiation via decreasing the expression of Olig 2, CNPase proteins in vitro. Moreover, Pb treatment inhibits the sodium/calcium exchanger 3 (NCX3) mRNA expression, one of the major means of calcium (Ca(2+)) extrusion at the plasma membrane during OPCs differentiation. Also addition of KB-R7943, NCX3 inhibitor, to simulate Pb toxicity, resulted in decreased myelin basic protein (MBP) expression and cell branching. Ca(2+) response trace with Pb and KB-R7943 treatment did not drop down in the same recovery time as the control, which elevated intracellular Ca(2+) concentration reducing MBP expression. In contrast, over-expression of NCX3 in Pb exposed OPCs displayed significant increase MBP fluorescence signal in positive regions and CNPase expression, which recovered OPCs differentiation to counterbalance Pb toxicity. In conclusion, Pb exposure disturbs OLs differentiation via affecting the function of NCX3 by inducing intracellular calcium overload.
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. matt0119@163.com.|Battalion 7 of Cadet Brigade, Third Military Medical University, Chongqing 400038, China. matt0119@163.com.
Teng
T
Ma
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. wuxiyan2003@163.com.
Xiyan
X
Wu
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. fengcai1112@126.com.
Qiyan
Q
Cai
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. yunwang30@hotmail.com.
Yun
Y
Wang
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. xiaolan35@hotmail.com.
Lan
L
Xiao
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. tianyp1981@163.com.
Yanping
Y
Tian
Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. lihongli@tmmu.edu.cn.
Hongli
H
Li
D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects*; D006801:Humans; D007855:Lead Poisoning / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D019831:Sodium-Calcium Exchanger / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology
C101670:2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate; D004676:Myelin Basic Protein; D012333:RNA, Messenger; C101832:SLC38A3 protein, human; D019831:Sodium-Calcium Exchanger; D013890:Thiourea; D002118:Calcium
Pb exposure; calcium signal; differentiation; oligodendrocytes; sodium/calcium exchanger 3