Molecular characterization of a ryanodine receptor gene from Spodoptera exigua and its upregulation by chlorantraniliprole.
Chlorantraniliprole is a novel diamide insecticide that targets the insect ryanodine receptor, a Ca(2+) release channel. Spodoptera exigua is a significant insect pest, and chlorantraniliprole is the most popular diamide insecticide used against this pest. To better understand the effects of diamides on RyR expression and [Ca(2+)], we isolated the SeRyR cDNA and investigated changes in SeRyR expression as a result of the application of chlorantraniliprole. The full-length cDNAs of SeRyR contain an open reading frame (ORF) of 15,357 bp with a predicted protein consisting of 5118 amino acids. SeRyR shares 77-92% identity with other insect RyR isoforms and 45-47% identity with vertebrate RyR isoforms. Furthermore, the relative expression abundances of RyR mRNA extracted from S. exigua fat body cells after 24 h of culture in 0.1, 1, 10, 100 nM, 1 µM and 100 µM of chlorantraniliprole changed 1.04-, 0.89-, 1.83-, 2.58-, 4.03- and 3.12-fold compared to blank control, respectively. The regression equation for the relative expression levels of SeRyR after 24 h as a function of the chlorantraniliprole concentration was Y = 0.6455 + 0.8188LgX, R(2) = 0.97093 for the cell line IOZCAS-Spex-II. These results outline the effects of chlorantraniliprole on the expression of SeRyR and provide a basis for the discovery of a compound that may exhibit selective insect activity.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China; Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning Province 125100, China.
Lina
L
Sun
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China; Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning Province 125100, China.
Guisheng
G
Qiu
Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Li
L
Cui
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China; Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Chunsen
C
Ma
Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: hzhyuan@ippcaas.cn.
Huizhu
H
Yuan
D000818:Animals; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics* / Q000378:metabolism; D018411:Spodoptera / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D019476:Insect Proteins; D007306:Insecticides; D019837:Ryanodine Receptor Calcium Release Channel; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole
Chlorantraniliprole; Expression; Ryanodine receptor; Spodoptera exigua