[The infection and molecular characteristics of Vibrio parahaemolyticus isolated from intestinal outpatient in two sentinel hospitals in Shanghai, 2010-2012].
OBJECTIVE
To analyze virulence genes and molecular characteristics of Vibrio parahaemolyticus isolated from sporadic cases with diarrhea in tow sentinel hospitals of Shanghai, 2010-2012.


METHODS
A total of 2 729 stool samples were collected from two surveillance sentinel hospitals in Shanghai 2010-2012. Vibrio parahaemolyticus strains were isolated and identified from diarrhea out patients using TCBS agar plates and biochemical reactions. Thermostable direct hemolysingene (tdh), thermostable-related hemolysin gene (trh), hemolysin gene (tlh) were detected by multiplex PCR method. Isolates were analyzed by PFGE and MLST. The PFGE profiles were analyzed using BioNumerics software.


RESULTS
A total of 30 clinical Vibrio parahaemolyticus strains isolated from 2 729 stool samples. The anually Vibrio parahaemolyticus isolation rate during 2010 to 2012 were 1.1%(11/973), 1.0%(11/1 120) and 1.3%(8/636) respectively. The PCR positive rates of virulence genes tlh, tdh and trh were 100%, 97% and 0 respectively. The Vibrio parahaemolyticus strains were divided into 13 PFGE types (P1-P13)and 3 ST types (ST-189, ST-799, ST-3). Among 13 PFGE types, P4 was the main PFGE type, accounting for 30%(9/30). P9, P10 were accounting for 12% (4/30) respectively, P1, P2, P12, P13 were accounting for 7%(2/30) respectively, the others types were 3%(1/30) respectively. MLST analysis results showed there are three ST types, ST3 was 84%(25/30), ST189 and ST799 were accounting for 13% (4/30) and 3% (1/30) respectively.


CONCLUSIONS
The infection rate of Vibrio parahaemolyticus was not very high from 2010-2012 in Shanghai, all strains were positive for tlh and negative for trh. ST3 was the major type of Vibrio parahaemolyticus.
Shanghai Public Health Clinical Center, Key Lab of Medical Molecular Virology, Pathogen Diagnosis and Biosafety Department, Shanghai 200000, China.
Haili
H
Chen
Haijian
H
Zhou
Shubei
S
Zai
Jinfeng
J
Cai
Fangxing
F
Qian
Liang
L
Ma
Moying
M
Wang
Zhen
Z
Shen
Yang
Y
Li
Jun
J
Zhang
Yunwen
Y
Hu
Biao
B
Kan
Email: zhaoqinzhu@163.com.
Zhaoqin
Z
Zhu
D002681:China; D003967:Diarrhea; D005838:Genotype; D006460:Hemolysin Proteins; D006761:Hospitals; D006801:Humans; D058885:Multilocus Sequence Typing; D010045:Outpatients; D016133:Polymerase Chain Reaction; D018571:Sentinel Surveillance; D014735:Vibrio Infections; D014736:Vibrio parahaemolyticus; D014774:Virulence
D006460:Hemolysin Proteins