Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid in Rabbit Osteoarthritis Model.
BACKGROUND
To investigate the effects of AOD9604 intra-articular injections with or without hyaluronic acid (HA) in a collagenase-induced knee osteoarthritis (OA) rabbit model.


METHODS
Mature New Zealand white rabbits (n=32) were randomly administered 2 mg collagenase type II twice in each knee joint. Weekly injections of 0.6 mL saline (Group 1), 6 mg HA (Group 2), 0.25 mg AOD9604 (Group 3), and 0.25 mg AOD9604 with 6 mg HA (Group 4) were administered for 4-7 weeks after the first intra-articular collagenase injection. The degree of cartilage degeneration was assessed using morphological and histopathological findings, and the degree of lameness was observed at 8 weeks after the first collagenase injection.


RESULTS
Mean gross morphological and histopathological scores were significantly higher in Group 1 than in Groups 2, 3, and 4, and the scores were significantly lower in Group 4 than in Groups 2 and 3. The lameness period in Group 4 was significantly shorter than those in Groups 1, 2, and 3. The lameness period in Group 1 was significantly longer than those in Groups 2 and 3.


CONCLUSIONS
Intra-articular AOD9604 injections using ultrasound guidance enhanced cartilage regeneration, and combined AOD9604 and HA injections were more effective than HA or AOD9604 injections alone in the collagenase-induced knee OA rabbit model.
Department of Rehabilitation Medicine, Catholic University of Daegu School of Medicine, Daegu, South Korea dongrakkwon@hotmail.com coolkwon@cu.ac.kr.
Dong Rak
DR
Kwon
Department of Rehabilitation Medicine, Catholic University of Daegu School of Medicine, Daegu, South Korea.
Gi Young
GY
Park
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D017364:Collagenases / Q000633:toxicity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D007270:Injections, Intra-Articular / Q000379:methods; D008297:Male; D010003:Osteoarthritis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D010446:Peptide Fragments / Q000008:administration & dosage*; D011817:Rabbits; D013004:Somatostatin / Q000008:administration & dosage*; D013997:Time Factors
C441619:AOD 9604; D000276:Adjuvants, Immunologic; D000893:Anti-Inflammatory Agents; D010446:Peptide Fragments; D013004:Somatostatin; D006820:Hyaluronic Acid; D017364:Collagenases
AOD9604; Collagenase; HA; Knee; Osteoarthritis; Ultrasound