Dragon fruit-like biocage as an iron trapping nanoplatform for high efficiency targeted cancer multimodality imaging.
Natural biopolymer based multifunctional nanomaterials are perfect candidates for multimodality imaging and therapeutic applications. Conventional methods of building multimodal imaging probe require either cross-linking manners to increase its in vivo stability or attach a target module to realize targeted imaging. In this study, the intrinsic photoacoustic signals and the native strong chelating properties with metal ions of melanin nanoparticle (MNP), and transferrin receptor 1 (TfR1) targeting ability of apoferritin (APF) was employed to construct an efficient nanoplatform (AMF) without tedious assembling process. Smart APF shell significantly increased metal ions loading (molar ratio of 1:800, APF/Fe(3+)) and therefore improved magnetic resonance imaging (MRI) sensitivity. Moreover, synergistic use of Fe(3+) and APF contributed to high photoacounstic imaging (PAI) sensitivity. AMF showed excellent bio-stability and presented good in vivo multimodality imaging (PET/MRI/PAI) properties (good tumor uptake, high specificity and high tumor contrast) in HT29 tumor because of its targeting property combined with the enhanced permeability and retention (EPR) effect, making it promising in theranostics and translational nanomedicine.
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China; Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA.
Min
M
Yang
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA.
Quli
Q
Fan
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA.
Ruiping
R
Zhang
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA.
Kai
K
Cheng
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China.
Junjie
J
Yan
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China.
Donghui
D
Pan
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA.
Xiaowei
X
Ma
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA.
Alex
A
Lu
Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, CA 94305-5484, USA. Electronic address: zcheng@stanford.edu.
Zhen
Z
Cheng
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001052:Apoferritins / Q000737:chemistry* / Q000378:metabolism; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007502:Iron Chelating Agents / Q000737:chemistry* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008543:Melanins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D064847:Multimodal Imaging; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D061088:Photoacoustic Techniques; D049268:Positron-Emission Tomography; D011990:Receptors, Transferrin / Q000378:metabolism
D015703:Antigens, CD; C068126:CD71 antigen; D007502:Iron Chelating Agents; D008543:Melanins; D011990:Receptors, Transferrin; D001052:Apoferritins
Apoferritin; MRI; Melanin; PAI; PET