First-Principles Investigation of Nanopore Sequencing Using Variable Voltage Bias on Graphene-Based Nanoribbons.
In this study, we examine the mechanism of nanopore-based DNA sequencing using a voltage bias across a graphene nanoribbon. Using density function theory and a nonequilibrium Green's function approach, we determine the transmission spectra and current profile for adenine, guanine, cytosine, thymine, and uracil as a function of bias voltage in an energy minimized configuration. Utilizing the transmission current, we provide a general methodology for the development of a three nanopore graphene-based device that can be used to distinguish between the various nucleobases for DNA/RNA sequencing. From our analysis, we deduce that it is possible to use different transverse currents across a multinanopore device to differentiate between nucleobases using various voltages of 0.5, 1.3, and 1.6 V. Overall, our goal is to improve nanopore design to further DNA/RNA nucleobase sequencing and biomolecule identification techniques.
†Department of Biology, James Madison University, Harrisonburg, Virginia 22802, United States.
Hannah L
HL
McFarland
‡Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.
Towfiq
T
Ahmed
‡Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.|§Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.
Jian-Xin
JX
Zhu
∥Institute of Material Science, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.|⊥Nordic Institute for Theoretical Physics, KTH Royal Institute of Technology and Stockholm University, Roslagstullsbacken 23, 106 91 Stockholm, Sweden.
Alexander V
AV
Balatsky
¶Department of Physics and Astronomy, James Madison University, Harrisonburg, Virginia 22802, United States.
Jason T
JT
Haraldsen
D006108:Graphite / Q000737:chemistry*; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*
D006108:Graphite
DNA/RNA sequencing; density functional theory; nanopore-based technology; nucleobase identification; transmission current