A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results.
Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study.
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Caio Nunes
CN
de Barros
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Ana Lúcia
AL
Miluzzi Yamada
Center for the Study of Venoms and Venomous Animals, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Rui Seabra F
RS
Junior
Center for the Study of Venoms and Venomous Animals, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Benedito
B
Barraviera
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Carlos Alberto
CA
Hussni
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Jaqueline Brandão
JB
de Souza
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Marcos Jun
MJ
Watanabe
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil.
Celso Antônio
CA
Rodrigues
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil anaalves@fmvz.unesp.br.
Ana Liz
AL
Garcia Alves
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D003435:Crotalid Venoms / Q000737:chemistry* / Q000302:isolation & purification; D017839:Crotalus; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D012756:Sheep; D054457:Tissue Scaffolds
D003435:Crotalid Venoms; D015718:Fibrin Tissue Adhesive
Fibrin gel; fibrin sealant; rattlesnake venom; scaffold