Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides.
We describe a protocol for the preparation of hybrid materials based on highly porous coordination polymer coatings on the internal surface of macroporous polymer monoliths. The developed approach is based on the preparation of a macroporous polymer containing carboxylic acid functional groups and the subsequent step-by-step solution-based controlled growth of a layer of a porous coordination polymer on the surface of the pores of the polymer monolith. The prepared metal-organic polymer hybrid has a high specific micropore surface area. The amount of iron(III) sites is enhanced through metal-organic coordination on the surface of the pores of the functional polymer support. The increase of metal sites is related to the number of iterations of the coating process. The developed preparation scheme is easily adapted to a capillary column format. The functional porous polymer is prepared as a self-contained single-block porous monolith within the capillary, yielding a flow-through separation device with excellent flow permeability and modest back-pressure. The metal-organic polymer hybrid column showed excellent performance for the enrichment of phosphopeptides from digested proteins and their subsequent detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presented experimental protocol is highly versatile, and can be easily implemented to different organic polymer supports and coatings with a plethora of porous coordination polymers and metal-organic frameworks for multiple purification and/or separation applications.
The Molecular Foundry, E. O. Lawrence Berkeley National Laboratory.
Alexandros
A
Lamprou
The Molecular Foundry, E. O. Lawrence Berkeley National Laboratory; Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology.
Hongxia
H
Wang
Institute of Chemical Sciences, Bahauddin Zakariya University.
Adeela
A
Saeed
The Molecular Foundry, E. O. Lawrence Berkeley National Laboratory.
Frantisek
F
Svec
The Molecular Foundry, E. O. Lawrence Berkeley National Laboratory; davidkbritt@gmail.com.
David
D
Britt
Department of Chemistry, University of the Balearic Islands.
Fernando
F
Maya
D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry*; D005290:Ferric Compounds; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D016062:Porosity
D056831:Coordination Complexes; D005290:Ferric Compounds; D010748:Phosphopeptides; D011108:Polymers