Effect of micropatterned TiO2 nanotubes thin film on the deposition of endothelial extracellular matrix: For the purpose of enhancing surface biocompatibility.
The vascular endothelial cells (EC) extracellular matrix (ECM) on the biomaterial surface can significantly improve the blood compatibility and cell compatibility of the cardiovascular materials. In the present study, two types of micropatterned TiO2 nanotubes surfaces (gronano and toponano) were fabricated on the titanium surface by photolithography and two-step anodizing technology, for the purpose of enhancing the deposition and loading ability of the EC ECM. The effect of the micropatterned nanotubes on EC ECM deposition and loading was investigated by qualitative and quantitative characterizations of type IV collagen (CoIV). The blood compatibility of the deposited ECM layers was evaluated by platelet adhesion and activation tests, and the endothelialization function of the deposited ECM layers was investigated by EC culture for 3 days. As a result, there was more CoIV on the toponano surface compared with the control. Meanwhile, the ECM loaded toponano (ECM/toponano) possessed better blood compatibility and better endothelialization than the control. This ECM loaded micro-/nanocomposite thin film was anticipated for the potential application of the surface modification of cardiovascular devices based on its excellent biocompatibility.
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Juejue
J
Wu
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Jingan
J
Li
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Feng
F
Wu
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Zikun
Z
He
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Ping
P
Yang
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
Nan
N
Huang
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D042783:Endothelial Cells / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007231:Infant, Newborn; D015539:Platelet Activation; D011485:Protein Binding; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; C009495:titanium dioxide; D014025:Titanium