Biodegradation of kerosene: Study of growth optimization and metabolic fate of P. janthinellum SDX7.
Penicillum janthinellum SDX7 was isolated from aged petroleum hydrocarbon-affected soil at the site of Anand, Gujarat, India, and was tested for different pH, temperature, agitation and concentrations for optimal growth of the isolate that was capable of degrading upto 95%, 63% and 58% of 1%, 3% and 5% kerosene, respectively, after a period of 16 days, at optimal growth conditions of pH 6.0, 30 °C and 180 rpm agitation. The GC/MS chromatograms revealed that then-alkane fractions are easily degraded; however, the rate might be lower for branched alkanes, n-alkylaromatics, cyclic alkanes and polynuclear aromatics. The test doses caused a concentration-dependent depletion of carbohydrates of P. janthinellum SDX7 by 3% to 80%, proteins by 4% to 81% and amino acids by 8% to 95% upto 16 days of treatment. The optimal concentration of 3% kerosene resulted in the least reduction of the metabolites of P. janthinellum such as carbohydrates, proteins and amino acids with optimal growth compared to 5% and 1% (v/v) kerosene doses on the 12(th) and 16(th) day of exposure. Phenols were found to be mounted by 43% to 66% at lower and higher concentrations during the experimental period. Fungal isolate P. janthinellum SDX7 was also tested for growth on various xenobiotic compounds.
Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Gujarat, India, Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Gujarat, India.
Shamiyan R
SR
Khan
Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Gujarat, India, Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Gujarat, India.
J I Kumar
JI
Nirmal
Department of Biological and Environmental Sciences, Natubhai V Patel College, Gujarat, India, Department of Biological and Environmental Sciences, Natubhai V Patel College, Gujarat, India.
Rita N
RN
Kumar
Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Gujarat, India, Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Gujarat, India.
Jignasha G
JG
Patel
D001482:Base Composition; D001711:Biotransformation; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007194:India; D007648:Kerosene; D008969:Molecular Sequence Data; D010407:Penicillium / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature; D015262:Xenobiotics / Q000378:metabolism*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D007648:Kerosene; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D012989:Soil Pollutants; D015262:Xenobiotics
Penicillum janthinellum SDX7; kerosene degradation; metabolites; optimal growth