Multimode sensors as new tools for molecular recognition of testosterone, dihydrotestosterone and estradiol in children's saliva.
Increased levels of testosterone (T2 ), dihydrotestosterone (DHT) and estradiol (E2 ) in children may be responsible for their early/delayed puberty and obesity conditions. Therefore, multimode sensors based on carbon matrices, such as graphite, graphene, fullerene C60 and multiwall carbon nanotubes modified with maltodextrin, were designed to assess reliably T2 , DHT and E2 in children saliva. The modes used for the assay of hormones were stochastic mode (for qualitative and quantitative determination of hormones) and differential pulse voltammetry mode (for quantitative determination of hormones). The advantage of this type of sensors, for hormone analysis, is their possibility to reach low concentration levels- are placed for children saliva under the detection limit of standard methods (e.g. ELISA used for the determination of these hormones in saliva). This made the multimode sensors an excellent tool for clinical analysis and especially for determination of substances of clinical importance in saliva samples. The proposed method is fast and simple, and no sampling of saliva is required.
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.|Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
Livia Alexandra
LA
Gugoasa
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.|Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
Raluca-Ioana
RI
Stefan-van Staden
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
Bogdan
B
Calenic
Institute for Environmental Studies, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, the Netherlands.
Juliette
J
Legler
D015374:Biosensing Techniques; D002648:Child; D002675:Child, Preschool; D013196:Dihydrotestosterone / Q000032:analysis*; D004958:Estradiol / Q000032:analysis*; D005260:Female; D006108:Graphite / Q000737:chemistry; D006801:Humans; D008297:Male; D043942:Nanotubes / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D013269:Stochastic Processes; D013739:Testosterone / Q000032:analysis*
D011134:Polysaccharides; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D006108:Graphite; C008315:maltodextrin
differential pulse voltammetry; hormones; multimode sensors; obesity; stochastic sensing