Separation of phenolic acids and flavonoids from Trollius chinensis Bunge by high speed counter-current chromatography.
In this work, eleven compounds were successfully separated from Trollius chinensis Bunge by using a two-step high-speed counter-current chromatography (HSCCC) method. NRTL-SAC (nonrandom two-liquid segment activity coefficient) method, a newly developed solvent system selection strategy, was applied to screening the suitable biphasic liquid systems. Hexane/ethyl acetate/ethanol/water (3:7:3:7, v/v) solvent system was used in the first step, while the hexane/ethyl acetate/methanol/water (1:2:1:2, 1:4:1:4, 1:9:1:9, v/v) systems were employed in the second step. The chemical structures of the separated compounds were identified by UV, high resolution ESI-MS and MS/MS data. The separated compounds are 3,4-dihydroxyphenylethanol (1), vanillic acid (2), orientin (3), vitexin (4), veratric acid (5), 2″-O-(3‴, 4‴-dimethoxybenzoyl) orientin (6), 2″-O-feruloylorientin (7), 2″-O-feruloylvitexin (8), 2″-O-(2‴-methylbutyryl) vitexin (9), 2″-O-(2‴-methylbutyryl) isoswertiajaponin (10), 2″-O-(2‴-methylbutyryl) isoswertisin (11). The results demonstrate that HSCCC is a powerful tool for the separation of compounds from extremely complex samples.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Yanhua
Y
Qin
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: yizeng_liang@263.net.
Yizeng
Y
Liang
Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming 650500, PR China.
Dabing
D
Ren
Department of Pharmaceutical Science, Medical College of Hunan Normal University, Changsha 410013, PR China.
Ximin
X
Qiu
Department of Pharmaceutical Science, Medical College of Hunan Normal University, Changsha 410013, PR China.
Xi
X
Li
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification*; D062385:Hydroxybenzoates / Q000302:isolation & purification*; D029626:Ranunculaceae / Q000737:chemistry*; D012997:Solvents
D005419:Flavonoids; D062385:Hydroxybenzoates; D012997:Solvents; C017616:phenolic acid
Flavonoids; HSCCC; Phenolic acids; Separation; Trollius chinensis Bunge