Quadruplex Integrated DNA (QuID) Nanosensors for Monitoring Dopamine.
Dopamine is widely innervated throughout the brain and critical for many cognitive and motor functions. Imbalances or loss in dopamine transmission underlie various psychiatric disorders and degenerative diseases. Research involving cellular studies and disease states would benefit from a tool for measuring dopamine transmission. Here we show a Quadruplex Integrated DNA (QuID) nanosensor platform for selective and dynamic detection of dopamine. This nanosensor exploits DNA technology and enzyme recognition systems to optically image dopamine levels. The DNA quadruplex architecture is designed to be compatible in physically constrained environments (110 nm) with high flexibility, homogeneity, and a lower detection limit of 110 µM.
Department of Pharmaceutical Sciences, Northeastern University, 206 The Fenway, 360 Huntington Avenue, Boston, MA 02115, USA. morales.je@husky.neu.edu.
Jennifer M
JM
Morales
Department of Pharmaceutical Sciences, Northeastern University, 206 The Fenway, 360 Huntington Avenue, Boston, MA 02115, USA. c.skipwith@neu.edu.
Christopher G
CG
Skipwith
Department of Pharmaceutical Sciences, Northeastern University, 206 The Fenway, 360 Huntington Avenue, Boston, MA 02115, USA. h.clark@neu.edu.
Heather A
HA
Clark
D000363:Agaricales / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis*; D054856:G-Quadruplexes; D014442:Monophenol Monooxygenase / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016624:Negative Staining; D011166:Porphyrins / Q000737:chemistry; D013057:Spectrum Analysis
D011166:Porphyrins; C107018:porphine; D014442:Monophenol Monooxygenase; D004298:Dopamine
DNA; dendrimer; dopamine; enzyme; nanosensor; tyrosinase