Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging.
Following our early discovery of arginylation in 1963, we have performed several studies to correlate its activity with essential biological processes. We employed cell- and tissue-based assays to detect both the level of acceptor proteins and the level of ATE1 activity under different conditions. Remarkably, in these assays, we found a close correlation between arginylation and aging, a discovery that we believe has longer-term implications in uncovering the importance of ATE1 in normal biology and disease therapies. Here we describe the original methods we used to measure ATE1 activity in tissues and correlate it with key biological events.
Department of Biochemistry and Molecular Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust St., Philadelphia, PA, 19107, USA, hideko.kaji@jefferson.edu.
Hideko
H
Kaji
Akira
A
Kaji
D000255:Adenosine Triphosphate / Q000378:metabolism; D019881:Aminoacyltransferases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D001126:Arginine-tRNA Ligase / Q000378:metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D002843:Chromatin / Q000378:metabolism; D004789:Enzyme Activation; D008861:Microsomes / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012347:RNA, Transfer, Arg / Q000235:genetics / Q000378:metabolism; D051381:Rats
D002843:Chromatin; D012347:RNA, Transfer, Arg; D000255:Adenosine Triphosphate; D001120:Arginine; D019881:Aminoacyltransferases; D001126:Arginine-tRNA Ligase