Cytotoxicity, genotoxicity and antibacterial effectiveness of a bioceramic endodontic sealer.
OBJECTIVE
To compare the characteristics of bioceramic endodontic sealer Endosequence BC sealer with those of AH Plus sealer.


METHODS
Cytotoxicity and genotoxicity were analysed on human gingival fibroblasts submitted to cell culture medium conditioned by sealers using the MTT reduction assay and micronucleus formation test (MNT), respectively. Cells grown on fresh medium served as controls. Cell viabilities were measured at 1, 3, 5 and 7 days. The antibacterial activity was analysed on an Enterococcus faecalis strain (ATCC 29212) using both on agar diffusion test (ADT) and a direct contact test (DCT). The inhibition zones in ADT were measured after 48 h and the colony-forming units counting in the DCT after 1, 24, 72 and 168 h. Data were compared by anova and Tukey's test and MNT by Fisher's exact test (P < 0.05).


RESULTS
Cultures submitted to Endosequence BC sealer had a significantly higher number of viable cells (P < 0.01) and less micronucleus formation (P < 0.05) than AH Plus sealer. Endosequence BC sealer exhibited significantly smaller inhibition zones (6.00 ± 0.03 mm) than AH Plus sealer (10.31 ± 0.21 mm) (P < 0.05). Moreover, Endosequence BC sealer had significantly smaller antibacterial activity than AH Plus sealer up to 1 h of direct contact (P < 0.05). On other exposure times, both materials had similar antibacterial effectiveness (P > 0.05).


CONCLUSIONS
Bioceramic-based sealer had less cytotoxicity and genotoxicity and similar antibacterial effect against E. faecalis in comparison with AH Plus sealer.
Department of Restorative Dentistry, Faculty of Dentistry, Universidade de São Paulo, São Paulo, Brazil.|Department of Dentistry, Centro Universitário Unichristus, Fortaleza, Brazil.
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Candeiro
Department of Restorative Dentistry, Faculty of Dentistry, Universidade de São Paulo, São Paulo, Brazil.|Graduate Program in Dentistry, Faculty of Dentistry, Instituto de Odontologia - Universidade Cruzeiro do Sul, São Paulo, Brazil.
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Moura-Netto
Department of Restorative Dentistry, Faculty of Dentistry, Universidade de São Paulo, São Paulo, Brazil.|Campus Castanhal, Universidade Federal do Pará, Castanhal, Brazil.
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D'Almeida-Couto
Department of Restorative Dentistry, Faculty of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
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Azambuja-Júnior
Department of Restorative Dentistry, Faculty of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
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Marques
Discipline of Microbiology, Biomedicine Sciences Institute, Universidade de São Paulo, São Paulo, Brazil.
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Cai
Department of Restorative Dentistry, Faculty of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
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Gavini
antibacterial effectiveness; bioceramic endodontic sealer; cell culture; cytotoxicity; genotoxicity