Chemical structure and biological activity of a quorum sensing pheromone from Bacillus subtilis subsp. natto.
Bacillus subtilis subsp. natto secrets a peptide pheromone, named ComXnatto pheromone, as an inducer for biofilm formation containing poly-γ-glutamic acid. Recently, the ComXnatto pheromone was identified to be a hexapeptide with an amino acid sequence of Lys-Trp-Pro-Pro-Ile-Glu, and the tryptophan residue was post-translationally modified with a farnesyl group. In order to determine the precise modification of the tryptophan residue, ComXnatto pheromone was synthesized using solid-phase peptide synthesis. Biological activity of the ComXnatto pheromone was then investigated. It was demonstrated that poly-γ-glutamic acid production were accelerated by ComXnatto pheromone at more than 1 nM in natto.
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: okadam@mol.f.u-tokyo.ac.jp.
Masahiro
M
Okada
Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Yuta
Y
Nakamura
Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Shunsuke
S
Hayashi
Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Koki
K
Ozaki
Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Syohei
S
Usami
D001412:Bacillus subtilis / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010675:Pheromones / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D053038:Quorum Sensing
D010675:Pheromones; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid
Bacillus subtilis; Natto; Posttranslational modification; Quorum sensing