Antimicrobial activity and synergism of Sami-Hyanglyun-Hwan with ciprofloxacin against methicillin-resistant Staphylococcus aureus.
OBJECTIVE
To investigate the antibacterial activity of SHH extracted with either water or ethanol against methicillin-resistant Staphylococcus aureus (MRSA) and combinatory antimicrobial effect with ciprofloxacin (CIP) by time kill assay and checkerboard dilution test.


METHODS
The antibacterial activity determined by broth dilution method indicated that the antibacterial activity of Sami-Hyanglyun-Hwan (SHH) water extract (SHHW) and SHH ethanol extract (SHHE) ranged from 250 to 2000 μg/mL and 125 to 1000 μg/mL against MRSA, respectively.


RESULTS
In the checkerboard method, the combinations of SHHE with CIP had a partial synergistic or synergistic effect against MRSA. The time-kill curves showed that a combined SHHE and CIP treatment reduced the bacterial counts dramatically after 24 h.


CONCLUSIONS
The present study demonstrates the therapeutic ability of SHHE against MRSA infections.
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk 570-749, Republic of Korea; Center of Excellence in Infectious Disease Research, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, ElPaso, TX 79905, USA.
Jang-Gi
JG
Choi
Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Ji-Young
JY
Choi
BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Su-Hyun
SH
Mun
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk 570-749, Republic of Korea.
Ok-Hwa
OH
Kang
Center of Excellence in Infectious Disease Research, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, ElPaso, TX 79905, USA.
Preeti
P
Bharaj
Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea.
Dong-Won
DW
Shin
Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Myong-Soo
MS
Chong
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk 570-749, Republic of Korea. Electronic address: sssimi@wku.ac.kr.
Dong-Yeul
DY
Kwon
Ciprofloxacin; Methicillin-resistant Staphylococcus aureus; Sami-Hyanglyun-Hwan; Synergy