Mucilaginibacter ginsengisoli sp. nov., isolated from a ginseng-cultivated soil.
A dark-pink-coloured bacterial strain, B4Y-8T, was isolated from a soil cultivated with ginseng. The 16S rRNA gene sequence of this strain showed highest similarity with Mucilaginibacter litoreus BR-18T (96.8 %), Mucilaginibacter lutimaris BR-3T (96.6 %) and Mucilaginibacter defluvii A5T (96.2 %) among the type strains of species of the genus Mucilaginibacter. Strain B4Y-8T was a strictly aerobic, Gram-stain-negative, non-motile, short-rod-shaped bacterium producing a large amount of extracellular polymeric substance. The strain grew at 10-35 °C (optimum, 25 °C), at pH 3.0-11.0 (optimum, pH 7.0) and in the presence of 0-1 % (w/v) NaCl (optimum, 0 %). The DNA G+C content of strain B4Y-8T was 49.0 mol%. It contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C15 : 0 as the major fatty acids. On the basis of evidence from the present polyphasic taxonomic study, strain B4Y-8T should be classified as representing a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter ginsengisoli sp. nov. is proposed. The type strain is B4Y-8T ( = KACC 18152T = JCM 30759T).
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Wanju 565-851, Republic of Korea.
Jae-Hyung
JH
Ahn
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Byoung-Chan
BC
Kim
Agricultural Research Institute for Climate Change, National Institute of Horticultural & Herbal Science, RDA, Jeju 690-150, Republic of Korea.
Jae-Ho
JH
Joa
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Wanju 565-851, Republic of Korea.
Soo-Jin
SJ
Kim
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Wanju 565-851, Republic of Korea.
Jaekyeong
J
Song
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Wanju 565-851, Republic of Korea.
Soon-Wo
SW
Kwon
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Wanju 565-851, Republic of Korea.
Hang-Yeon
HY
Weon
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005894:Panax; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7