A novel imaging tool for hepatic portal system using phase contrast technique with hydrogen peroxide-generated O₂ gas.
The objective of this study was to investigate the potential of hydrogen peroxide-generated oxygen gas-based phase contrast imaging (PCI) for visualizing mouse hepatic portal veins. The O2 gas was made from the reaction between H2O2 and catalase. The gas production was imaged by PCI in real time. The H2O2 was injected into the enteric cavity of the lower sigmoid colon to produce O2 in the submucosal venous plexus. The generated O2 gas could be finally drained into hepatic portal veins. Absorption contrast imaging (ACI) and PCI of O2-filled portal veins were performed and compared. PCI offers high resolution and real-time visualization of the O2 gas production. Compared with O2-based ACI, O2-based PCI significantly enhanced the revealing of the portal vein in vivo. It is concluded that O2-based PCI is a novel and promising imaging modality for future studies of portal venous disorders in mice models.
Department of Radiology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Rongbiao
R
Tang
Department of Radiology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Fuhua
F
Yan
Department of Radiology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Wei Min
WM
Chai
Department of Radiology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Wei
W
Huang
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, People's Republic of China.
Yanan
Y
Fu
Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Guo Yuan
GY
Yang
Department of Radiology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Ke Min
KM
Chen
D000818:Animals; D002374:Catalase / Q000097:blood; D012809:Colon, Sigmoid; D003199:Computer Systems; D003287:Contrast Media; D006861:Hydrogen Peroxide; D007267:Injections; D051379:Mice; D008813:Mice, Inbred ICR; D045423:Microbubbles; D010100:Oxygen; D010690:Phlebography / Q000379:methods*; D011168:Portal System / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D011169:Portal Vein / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D017356:Synchrotrons
D003287:Contrast Media; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen
O2 gas; X-ray phase contrast imaging; hepatic portal vein; hydrogen peroxide