Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.
BACKGROUND
Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].


RESULTS
In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].


CONCLUSIONS
Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.
Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.|Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven - University of Leuven, 3000, Leuven, Belgium. karolina.laskowska.macios@gmail.com.
Karolina
K
Laskowska-Macios
Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven - University of Leuven, 3000, Leuven, Belgium. julie.nys@bio.kuleuven.be.
Julie
J
Nys
Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven - University of Leuven, 3000, Leuven, Belgium. tjing-tjing.hu@thrombogenics.com.
Tjing-Tjing
TT
Hu
Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. m.zapasnik@nencki.gov.pl.
Monika
M
Zapasnik
Laboratory for Neurobiology and Gene Therapy, KU Leuven - University of Leuven, 3000, Leuven, Belgium. Anke.VanderPerren@med.kuleuven.be.
Anke
A
Van der Perren
Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. kossut@nencki.gov.pl.
Malgorzata
M
Kossut
Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. k.burnat@nencki.gov.pl.
Kalina
K
Burnat
Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven - University of Leuven, 3000, Leuven, Belgium. lut.arckens@bio.kuleuven.be.
Lutgarde
L
Arckens
D000367:Age Factors; D000818:Animals; D015153:Blotting, Western; D002415:Cats; D002966:Clathrin / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004705:Endocytosis; D005136:Eye Proteins / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism; D012683:Sensory Deprivation; D009435:Synaptic Transmission; D015348:Vision, Binocular; D014793:Visual Cortex / Q000254:growth & development* / Q000378:metabolism*
D002966:Clathrin; D005136:Eye Proteins; D050883:HSC70 Heat-Shock Proteins