Association between CTLA-4 gene polymorphism and ankylosing spondylitis: a case-control study.
OBJECTIVE
The aim of our study was to evaluate the association between CTLA-4 polymorphisms (+49A/G, -318C/T and CT60A/G) and ankylosing spondylitis (AS) susceptibility.


METHODS
A total of 120 AS cases and healthy controls, matched on the age and gender, were enrolled in the study. Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) were used to determine the gentypes of +49A/G, -318C/T and CT60A/G polymorphisms. Genotype distribution in control group was assessed by Hardy Weinberg Equilibrium (HWE) test. Odds ratio (OR) with 95% confidence interval (95% CI) were adopted to evaluate the relationship of CTLA-4 polymorphisms and AS susceptibility.


RESULTS
In our study, genotype distribution of the three polymorphisms in control group was consistent with the HWE (P > 0.05). The genotype analysis showed that AA genotype of + 49A/G polymorphism could increase the risk for AS (OR=2.357, 95% CI=1.127-4.930). Moreover, the frequency of A allele was also presented as a risk factor for AS. Additionally, AA genotype and A allele of CT60A/G appeared to be related with AS susceptibility (OR=2.610, 95% CI=1.047-6.510; OR=1.751, 95% CI=1.160-2.641). However, the T allele of -318C/T appeared to be a protective factor for AS (OR=0.383, 95% CI=0.228-0.643).


CONCLUSIONS
In summary, there existed significant association between CTLA-4 gene polymorphisms and increased or decreased risk for AS.
Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Nai-Guo
NG
Wang
Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Da-Chuan
DC
Wang
Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Bing-Yi
BY
Tan
Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Feng
F
Wang
Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Ze-Nong
ZN
Yuan
D000328:Adult; D044466:Asian People / Q000235:genetics; D060908:CTLA-4 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013167:Spondylitis, Ankylosing / Q000235:genetics*; D055815:Young Adult
D060908:CTLA-4 Antigen
+49A/G; -318C/T; CT60A/G; CTLA-4; ankylosing spondylitis