Distribution of mutations in DNMT3A gene and the suitability of mutations in R882 codon for MRD monitoring in patients with AML.
The DNA methyl-transferase 3A gene (DNMT3A) is the third most frequently mutated gene in cytogenetically normal acute myeloid leukemia (CN-AML) patients (20-30 %), who belong to a group of patients with intermediate risk. About 60 % of mutations in this gene have been identified in the arginine codon R882. To date, there is no consensus on whether these mutations can be used as biomarkers for monitoring of minimal residual disease and management of preemptive AML therapy. We studied the occurrence of mutations in the DNMT3A gene in our cohort of patients and their persistence during AML treatment. Using next-generation sequencing, we identified four mutations in 11/25 of our analyzed patients--frequent R882C and R882H mutations, rare Y735S mutation, and a novel L347P mutation. Mutation R882C was detected in 5/11, R882H in 4/11 patients, and Y735S and L347P in one patient each. In 4/7 patients initially carrying mutations in the R882 codon, we found the persistence of mutations also during complete remission with, however, no correlation to AML kinetics. Our findings suggest that mutations in the DNMT3A gene can only be used as a biomarker for those AML patients in whom DNMT3A mutation is lost after therapy.
Department of Internal Medicine, Hematology and Oncology, University Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.|Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Ivana
I
Jeziskova
Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Milena
M
Musilova
Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Martin
M
Culen
Department of Internal Medicine, Hematology and Oncology, University Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.|Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Veronika
V
Foltankova
Department of Internal Medicine, Hematology and Oncology, University Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.|Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Dana
D
Dvorakova
Department of Internal Medicine, Hematology and Oncology, University Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.|Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.|CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Jiri
J
Mayer
Department of Internal Medicine, Hematology and Oncology, University Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic. zracil@fnbrno.cz.|Department of Internal Medicine, Hematology and Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. zracil@fnbrno.cz.|CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic. zracil@fnbrno.cz.
Zdenek
Z
Racil
D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution; D014408:Biomarkers, Tumor / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D000090205:DNA Methyltransferase 3A; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic; D020125:Mutation, Missense; D009363:Neoplasm Proteins / Q000235:genetics*; D018365:Neoplasm, Residual
D014408:Biomarkers, Tumor; D003062:Codon; C000717967:DNMT3A protein, human; D009363:Neoplasm Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A
AML preleukemic stem cells; DNMT3A gene mutations; MRD; Next-generation sequencing