Effects of Surface-Deacetylated Chitin Nanofibers in an Experimental Model of Hypercholesterolemia.
This study evaluated the effects of oral administration of surface-deacetylated chitin nanofibers (SDACNFs) on hypercholesterolemia using an experimental model. All rats were fed a high cholesterol diet with 1% w/w cholesterol and 0.5% w/w cholic acid for 28 days. Rats were divided equally into four groups: the control group was administered 0.05% acetic acid dissolved in tap water, and the SDACNF, chitosan (CS), and cellulose nanofiber (CLNF) groups were administered 0.1% CNF, CS, or CLNF dissolved in the tap water, respectively, during the experimental period. Changes in body weight, intake of food and water, and organ weight were measured. Serum blood chemistry and histopathological examination of the liver were performed. Administration of SDACNF did not affect body weight change, food and water intake, or organ weights. Administration of SDACNF and CS decreased the diet-induced increase in serum total cholesterol, chylomicron, very-low-density lipoprotein, and phospholipid levels on day 14. Moreover, oral administration of SDACNFs suppressed the increase of alanine transaminase levels on day 29 and suppressed vacuolar degeneration and accumulation of lipid droplets in liver tissue. These data indicate that SDACNF has potential as a functional food for patients with hypercholesterolemia.
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. kazu-azuma@muses.tottori-u.ac.jp.
Kazuo
K
Azuma
Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. tomonekomame@gmail.com.
Tomone
T
Nagae
Japan Food Research Laboratories, Tama 206-0025, Japan. nagait@jfrl.or.jp.
Takeshi
T
Nagai
Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. h-izawa@chem.tottori-u.ac.jp.
Hironori
H
Izawa
Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University, Tottori 680-8550, Japan. morimoro@chem.tottori-u.ac.jp.
Minoru
M
Morimoto
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. ymurahata@muses.tottori-u.ac.jp.
Yusuke
Y
Murahata
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. tosaki@muses.tottori-u.ac.jp.
Tomohiro
T
Osaki
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. tsuka@muses.tottori-u.ac.jp.
Takeshi
T
Tsuka
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. imagawat@muses.tottori-u.ac.jp.
Tomohiro
T
Imagawa
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. taro@muses.tottori-u.ac.jp.
Norihiko
N
Ito
Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. yokamoto@muses.tottori-u.ac.jp.
Yoshiharu
Y
Okamoto
Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. saimoto@chem.tottori-u.ac.jp.
Hiroyuki
H
Saimoto
Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. sifuku@chem.tottori-u.ac.jp.
Shinsuke
S
Ifuku
D000284:Administration, Oral; D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage* / Q000737:chemistry; D002482:Cellulose / Q000008:administration & dosage / Q000737:chemistry; D002686:Chitin / Q000008:administration & dosage* / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage; D002784:Cholesterol / Q000097:blood; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000178:diet therapy* / Q000473:pathology; D008079:Lipoproteins, VLDL / Q000097:blood; D057139:Nanofibers / Q000008:administration & dosage*; D010743:Phospholipids / Q000097:blood; D051381:Rats
D000924:Anticholesteremic Agents; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D002686:Chitin; D002482:Cellulose; D048271:Chitosan; D002784:Cholesterol
chitin nanofibers; chitosan; hypercholesterolemia; rats; surface-deacetylated chitin nanofibers