Establishment of a bioluminescence model for microenvironmentally induced oral carcinogenesis with implications for screening bioengineered scaffolds.
Microenvironmental cues play a major role in head and neck cancer. Biodegradable scaffolds used for bone regeneration might also act as stimulative cues for head and neck cancer. The purpose of this study was to establish an experimental model for precise and noninvasive evaluation of tumorigenic potential of microenvironmental cues in head and neck cancer.



Bioluminescence was chosen to image tumor formation. Early neoplastic oral keratinocyte (DOK) cells were luciferase-transduced (DOK(Luc) ), then tested in nonobese diabetic severe combined immunodeficient IL2rγnull mice either orthotopically (tongue) or subcutaneously for their potential as "screening sensors" for diverse microenvironmental cues.



Tumors formed after inoculation of DOK(Luc) were monitored easier by bioluminescence, and bioluminescence was more sensitive in detecting differences between various microenvironmental cues when compared to manual measurements. Development of tumors from DOK(Luc) grown on scaffolds was also successfully monitored noninvasively by bioluminescence.



The model presented here is a noninvasive and sensitive model for monitoring the impact of various microenvironmental cues on head and neck cancer in vivo. © 2015 The Authors Head & Neck Published by Wiley Periodicals, Inc. Head Neck 38: E1177-E1187, 2016.
Department of Clinical Dentistry, Centre for Clinical Dental Research, University of Bergen, Bergen, Norway.|Gade Laboratory for Pathology, Department of Clinical Medicine, University of Bergen, Bergen, Norway.|Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
Salwa
S
Suliman
Gade Laboratory for Pathology, Department of Clinical Medicine, University of Bergen, Bergen, Norway.|Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
Himalaya
H
Parajuli
Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
Yang
Y
Sun
Gade Laboratory for Pathology, Department of Clinical Medicine, University of Bergen, Bergen, Norway.|Centre for Cancer Biomarkers, Department of Clinical Medicine, University of Bergen, Bergen, Norway.|Department of Pathology, Haukeland University Hospital, Bergen, Norway.
Anne Christine
AC
Johannessen
Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
Anna
A
Finne-Wistrand
Department of Clinical Science, University of Bergen, Bergen, Norway.|Department of Medicine, Haematology Section, Haukeland University Hospital, Bergen, Norway.
Emmet
E
McCormack
Department of Clinical Dentistry, Centre for Clinical Dental Research, University of Bergen, Bergen, Norway.
Kamal
K
Mustafa
Gade Laboratory for Pathology, Department of Clinical Medicine, University of Bergen, Bergen, Norway.|Centre for Cancer Biomarkers, Department of Clinical Medicine, University of Bergen, Bergen, Norway.|Department of Pathology, Haukeland University Hospital, Bergen, Norway.
Daniela Elena
DE
Costea
D000818:Animals; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D006258:Head and Neck Neoplasms / Q000000981:diagnostic imaging*; D008156:Luciferases; D008163:Luminescent Measurements; D008297:Male; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging; D054457:Tissue Scaffolds; D059016:Tumor Microenvironment
D008156:Luciferases
bioluminescence; cancer; microenvironment; scaffold; tissue engineering