A dual functional peptide carrying in vitro selected catalytic and binding activities.
When minimal functional sequences are used, it is possible to integrate multiple functions on a single peptide chain, like a "single stroke drawing". Here a dual functional peptide was designed by combining in vitro selected catalytic and binding activities. For catalytic activity, we performed in vitro selection for a peptide aptamer binding to hemin by using ribosome display and isolated a peptide that had peroxidase activity in the presence of hemin. By combining the selected catalytic peptide with a peptide antigen, which can be recognized by an antibody, an enzyme-antibody conjugate-like peptide was obtained. This study demonstrates a successful strategy to create dual functionalized peptide chains for use in immunoassays.
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Liping
L
Zhu
Wei
W
Wang
Haixu
H
Zhao
Muye
M
Xu
Seiichi
S
Tada
Takanori
T
Uzawa
Mingzhe
M
Liu
Yoshihiro
Y
Ito
D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D052158:Aptamers, Peptide / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002384:Catalysis; D006427:Hemin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D019151:Peptide Library; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*
D000906:Antibodies; D052158:Aptamers, Peptide; D009841:Oligonucleotides; D019151:Peptide Library; D006427:Hemin; D009195:Peroxidase