Capillary ion chromatography-mass spectrometry for simultaneous determination of glucosylglycerol and sucrose in intracellular extracts of cyanobacteria.
A capillary ion chromatography-mass spectrometry (MS) method was proposed to determine glucosylglycerol (GG), sucrose, and five other carbohydrates. MS conditions and make-up flow parameters were optimized. This method is accurate and sensitive for simultaneous analysis of carbohydrates, with mean correlation coefficients of determination greater than 0.99, relative standard deviation of 0.91-2.81% for eight replicates, and average spiked recoveries of 97.3-104.9%. Limits of detection of sodium adduct were obtained with MS detection in selected ion mode for GG (0.006mg/L), sucrose (0.02mg/L), and other carbohydrates (0.03mg/L). This method was successfully applied to determine GG and sucrose in intracellular extracts of salt-stressed cyanobacteria.
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: fayun@qibebt.ac.cn.
Yun
Y
Fa
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
Wenhui
W
Liang
Technical Center of Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70 Qutangxia Road, Qingdao 266002, China.
He
H
Cui
University of Chinese Academy of Sciences, Beijing 100049, China.
Yangkai
Y
Duan
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.
Menglong
M
Yang
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.
Jun
J
Gao
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.
Huizhou
H
Liu
D002853:Chromatography, Liquid / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D013395:Sucrose / Q000032:analysis*
D005960:Glucosides; C034727:glucosylglycerol; D013395:Sucrose
Capillary ion chromatography; Cyanobacteria; Glucosylglycerol; Mass spectrometry; sucrose