A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue.
A novel electrochemical immunosensor was fabricated for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA based on anti-5-hmC antibody, biotin functionalized phos-tag and avidin functionalized alkaline phosphatase. It is demonstrated that the levels of 5-hmC are dramatically reduced in human breast cancer tissue compared with those in normal tissue.
College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, P. R. China. yinhs@sdau.edu.cn ashy@sdau.edu.cn.
Zhiqing
Z
Yang
Wenjing
W
Jiang
Fei
F
Liu
Yunlei
Y
Zhou
Huanshun
H
Yin
Shiyun
S
Ai
D044503:5-Methylcytosine / Q000031:analogs & derivatives; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000235:genetics*; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004273:DNA, Neoplasm / Q000737:chemistry*; D005260:Female; D006801:Humans
D004273:DNA, Neoplasm; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine