Phenolic Compounds from Atriplex littoralis and Their Radiation-Mitigating Activity.
From the aerial parts of Atriplex littoralis, three new flavonoid glycosides named atriplexins I-III (1-3), a known flavonoid glycoside, spinacetin 3-O-β-d-glucopyranoside (4), arbutin (5), and 4-hydroxybenzyl-β-d-glucopyranoside (6) were isolated. Their structures were elucidated on the basis of detailed spectroscopic analysis, including 1D and 2D NMR (COSY, NOESY, TOCSY, HSQC, HMBC) and HRESITOF MS data. The compounds were tested for in vitro protective effects on chromosome aberrations in peripheral human lymphocytes using a cytochalasin-B-blocked micronucleus (MN) assay in a concentration range of 0.8-7.4 μM of final culture solution. Chromosomal damage was induced by 2 Gy of γ-radiation on binucleated human lymphocytes, and the effects of the compounds were tested 2 to 19 h after irradiation. The frequency of micronuclei (MNi) was scored in binucleated cells, and the nuclear proliferation index was calculated. The highest prevention of in vitro biochemical and cytogenetic damage of human lymphocytes induced by γ-radiation was exhibited by 3 (reduction of MN frequency by 31%), followed by 4 and 6.
Dejan
D
Gođevac
Jovana
J
Stanković
Miroslav
M
Novaković
Boban
B
Anđelković
Faculty of Agriculture, University of Belgrade , 6 Nemanjina Street, 11080 Zemun, Serbia.
Zora
Z
Dajić-Stevanović
Faculty of Agriculture, University of Belgrade , 6 Nemanjina Street, 11080 Zemun, Serbia.
Milica
M
Petrović
Miroslava
M
Stanković
D027466:Atriplex / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008296:Malaysia; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
D003374:Coumarins; D010636:Phenols