Serum peptidome profiling analysis for the identification of potential biomarkers in cervical intraepithelial neoplasia patients.
Cervical intraepithelial neoplasia (CIN) is a precancerous disease of cervical squamous cell carcinoma. We Used Mass Spectrometry based peptidome profile study to predict the transformation of CIN1, which is the primary stage of this lesion. . Serum samples of 34 Cervical squamous cell carcinoma patients, 31 healthy controls, and 29 CIN1 samples were analyzed. Peptides were purified by WCX magnetic beads (Bioyong), and analyzed by MALDI TOF (Bruker). Raw data were analyzed by BioExplorer software (Bioyong). The results showed 14 mass peaks with significant differences. The diagnosis model is established by analyzing peptide profiles of 15 SCC patients and 20 healthy women serum, with a sensitivity of 100% and speciﬁcity of 100.00%. In validation set, the SCC diagnosis model also had good performance with a sensitivity of 80%, a specificity of 100%. In addition, this model could predict 29 CIN1 patients with accuracy of 55.17%. These results would provide a new method to predict the trend of CIN1 and take effective measures for high risk group timely.
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
Yun
Y
Liu
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Fangqiao
F
Wei
Bioyong Technologies Inc., Beijing, China.
Feng
F
Wang
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
Changdong
C
Li
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
Ge
G
Meng
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
Hua
H
Duan
Bioyong Technologies Inc., Beijing, China.
Qingwei
Q
Ma
Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address: zhangwy9921@hotmail.com.
Weiyuan
W
Zhang
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000097:blood*; D020543:Proteome / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000378:metabolism*
D014408:Biomarkers, Tumor; D010455:Peptides; D020543:Proteome
CIN; Peptidome; WCX-MB