Antifungal activity of Brevibacillus laterosporus JX-5 and characterization of its antifungal components.
The establishment of safe and effective methods for controlling fungal disease is an urgent issue in agriculture and forestry. Microbiological control of plant disease is expected to achieve better results than use of chemically derived fungicides. This study aimed to establish Brevibacillus laterosporus JX-5 as a potential microbiological control agent of poplar canker. The bacterium was isolated from the poplar rhizosphere and demonstrated significant growth inhibition of several pathogenic fungi in vitro. The antifungal components of Br. laterosporus JX-5 were isolated and identified. The fermentation broth of Br. laterosporus JX-5 and its main antifungal component, designated as component B, reduced Botryosphaeria dothidea associated canker of the excised poplar branch by 70 and 90%, respectively. Component B is considerably heat-stable, adaptable to a broad pH range, and UV-resistant. It could inhibit Bo. dothidea by permeating the fungal membrane, fracturing the nuclei, damaging the cell wall, and eventually killing the pathogenic fungus. The antifungal activity exhibited by Br. laterosporus JX-5 and its bioactive metabolic products indicate its feasibility as a potential biocontrol agent for plant diseases.
College of Life Science, Shandong Agricultural University, Taian, 271018, China. lx100000y@163.com.|School of Life Science, Taishan Medical University, Taian, 271018, China. lx100000y@163.com.
Hongxia
H
Jiang
College of Life Science, Shandong Agricultural University, Taian, 271018, China.
Xiaohui
X
Wang
College of Forestry, Shandong Agricultural University, Taian, 271018, China.
Chengze
C
Xiao
College of Forestry, Shandong Agricultural University, Taian, 271018, China.
Weiyan
W
Wang
College of Forestry, Shandong Agricultural University, Taian, 271018, China.
Xu
X
Zhao
College of Life Science, Shandong Agricultural University, Taian, 271018, China.
Junkang
J
Sui
College of Life Science, Shandong Agricultural University, Taian, 271018, China.|School of Life Science, Taishan Medical University, Taian, 271018, China.
Rongbo
R
Sa
Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602-7382, USA. tlguo1@uga.edu.
Tai L
TL
Guo
College of Forestry, Shandong Agricultural University, Taian, 271018, China. xlliu@sdau.edu.cn.
Xunli
X
Liu
D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism*; D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development; D058675:Brevibacillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010539:Permeability / Q000187:drug effects; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D032107:Populus / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D000935:Antifungal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
Antifungal activity; Biological control; Bogorol; Botryosphaeria dothidea; Brevibacillus laterosporus JX-5