An innovative pre-targeting strategy for tumor cell specific imaging and therapy.
A programmed pre-targeting system for tumor cell imaging and targeting therapy was established based on the "biotin-avidin" interaction. In this programmed functional system, transferrin-biotin can be actively captured by tumor cells with the overexpression of transferrin receptors, thus achieving the pre-targeting modality. Depending upon avidin-biotin recognition, the attachment of multivalent FITC-avidin to biotinylated tumor cells not only offered the rapid fluorescence labelling, but also endowed the pre-targeted cells with targeting sites for the specifically designed biotinylated peptide nano-drug. Owing to the successful pre-targeting, tumorous HepG2 and HeLa cells were effectively distinguished from the normal 3T3 cells via fluorescence imaging. In addition, the self-assembled peptide nano-drug resulted in enhanced cell apoptosis in the observed HepG2 cells. The tumor cell specific pre-targeting strategy is applicable for a variety of different imaging and therapeutic agents for tumor treatments.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China. xz-zhang@whu.edu.cn.
Si-Yong
SY
Qin
Meng-Yun
MY
Peng
Lei
L
Rong
Hui-Zhen
HZ
Jia
Si
S
Chen
Si-Xue
SX
Cheng
Jun
J
Feng
Xian-Zheng
XZ
Zhang
D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001360:Avidin / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000188:drug therapy / Q000401:mortality / Q000473:pathology; D061848:Optical Imaging / Q000379:methods*; D011990:Receptors, Transferrin / Q000378:metabolism*
D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D011990:Receptors, Transferrin; D001360:Avidin; D001710:Biotin