Hyperpolarized (6)Li as a probe for hemoglobin oxygenation level.
Hyperpolarization by dissolution dynamic nuclear polarization (DNP) is a versatile technique to dramatically enhance the nuclear magnetic resonance (NMR) signal intensity of insensitive long-T1 nuclear spins such as (6)Li. The (6)Li longitudinal relaxation of lithium ions in aqueous solutions strongly depends on the concentration of paramagnetic species, even if they are present in minute amounts. We herein demonstrate that blood oxygenation can be readily detected by taking advantage of the (6)Li signal enhancement provided by dissolution DNP, together with the more than 10% decrease in (6)Li longitudinal relaxation as a consequence of the presence of paramagnetic deoxyhemoglobin.
Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
Riccardo
R
Balzan
Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.|Department of Radiology, Université de Lausanne, CH-1015, Lausanne, Switzerland.
Mor
M
Mishkovsky
School of Chemistry, Tel-Aviv University, Ramat-Aviv, Tel Aviv, Israel.
Yana
Y
Simonenko
Department of Radiology, Université de Lausanne, CH-1015, Lausanne, Switzerland.|Center of Biomedical Imaging (CIBM), Lausanne, Switzerland.
Ruud B
RB
van Heeswijk
Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.|Department of Radiology, Université de Lausanne, CH-1015, Lausanne, Switzerland.|Department of Radiology, Geneva University Hospital and Faculty of Medicine, University of Geneva, CH-1211, Genève 4, Switzerland.
Rolf
R
Gruetter
School of Chemistry, Tel-Aviv University, Ramat-Aviv, Tel Aviv, Israel.
Uzi
U
Eliav
School of Chemistry, Tel-Aviv University, Ramat-Aviv, Tel Aviv, Israel.
Gil
G
Navon
Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
Arnaud
A
Comment
D000818:Animals; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry; D008094:Lithium / Q000737:chemistry / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats
D003287:Contrast Media; D006454:Hemoglobins; D007477:Ions; C100003:deoxyhemoglobin; D008094:Lithium
MRI; blood; deoxyhemoglobin; dynamic nuclear polarization; hyperpolarization; lithium; magnetic resonance; molecular imaging