Total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease.
The first total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease is described by native chemical ligation. Chemical synthesis of azide-labeled proteins would provide useful protein tools for biochemical, biophysical or medical studies.
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China. jszheng@hmfl.cas.cn.
Yun-Kun
YK
Qi
Hao-Nan
HN
Chang
Kai-Mai
KM
Pan
Chang-Lin
CL
Tian
Ji-Shen
JS
Zheng
D000595:Amino Acid Sequence; D001386:Azides / Q000138:chemical synthesis / Q000737:chemistry*; D016333:HIV Protease / Q000737:chemistry*; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization
D001386:Azides; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1