[Immunochemical Detection of Azospirilla in Soil with Genus-Specific Antibodies].
Immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria Azospirillum brasilense Sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. Cross-reactions with these proteins made it possible to use the above antibodies for detection of azospirilla as a genus-specific probe conjugated with horseradish peroxidase as an enzymatic label. Direct immune-enzyme analysis of soil suspensions (typical chernozem, Saratov oblast) confirmed applicability of the conjugates based on genus-specific antibodies to the surface proteins of azospirilla for direct detection of this bacterial genus in environmental samples. These results provide a basis for broad application of this method for analysis of Azospirillum occurrence in soil.
A A
AA
Shirokov
A I
AI
Krasov
N Yu
NY
Selivanov
G L
GL
Burygin
S Yu
SY
Shchegolev
L Yu
LY
Matora
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D015806:Azospirillum brasilense / Q000276:immunology / Q000302:isolation & purification*; D006735:Horseradish Peroxidase / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification; D011817:Rabbits; D058441:Rhizosphere; D012988:Soil Microbiology
D000942:Antigens, Bacterial; D018796:Immunoconjugates; D007074:Immunoglobulin G; D006735:Horseradish Peroxidase