[Effect of intermittent hypoxia of sleep apnea on embryonic rat cortical neurons in vitro].
OBJECTIVE
To investigate the effects of different pattens of intermittent hypoxia on the activity and apoptosis of primary cultured rat embryonic cortical neurons, and to evaluate the role of intermittent hypoxia in the mechanism of obstructive sleep syndrom induced cognitive function loss.


METHODS
The embryonic cerebral cortical neurons were cultured in vitro and were identified by immunofluorescence. Cultured neurons were randomly divided into intermittent hypoxia group, intermittent normal oxygen group, persistent hypoxia group and the control group, and intermittent hypoxia group was divided into five subgroups according to different frequency and time-bound. Neurons were exposed in different modes of hypoxia. MTT colorimetry was used to detect the viability of the neurons, and DAPI colorated measurement was used to calculate the percentages of neuron apoptosis.


RESULTS
There were significantly different effects between all subgroups of intermittent hypoxia and the continued hypoxia group on neuronal activity and apoptosis (P < 0.01); Intermittent hypoxia groups with different frequency and time had no difference in neuronal activity and apoptosis (P > 0.05).


CONCLUSIONS
The effect of intermittent hypoxia was more serious than that of continued hypoxia on neuronal activity and apoptosis; The impact of intermittent hypoxia on neuronal activity and apoptosis may be an important factor in obstructive sleep apnea related cognitive impairment.
Chanjuan
C
Zhang
Yanzhong
Y
Li
Yan
Y
Wang
D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D002478:Cells, Cultured; D003072:Cognition Disorders; D009474:Neurons / Q000166:cytology*; D010100:Oxygen; D051381:Rats; D020181:Sleep Apnea, Obstructive
D010100:Oxygen