Microarray analysis of gene expression patterns in the leaf during potato tuberization in the potato somatic hybrid Solanum tuberosum and Solanum etuberosum.
Genes involved in photoassimilate partitioning and changes in hormonal balance are important for potato tuberization. In the present study, we investigated gene expression patterns in the tuber-bearing potato somatic hybrid (E1-3) and control non-tuberous wild species Solanum etuberosum (Etb) by microarray. Plants were grown under controlled conditions and leaves were collected at eight tuber developmental stages for microarray analysis. A t-test analysis identified a total of 468 genes (94 up-regulated and 374 down-regulated) that were statistically significant (p ≤ 0.05) and differentially expressed in E1-3 and Etb. Gene Ontology (GO) characterization of the 468 genes revealed that 145 were annotated and 323 were of unknown function. Further, these 145 genes were grouped based on GO biological processes followed by molecular function and (or) PGSC description into 15 gene sets, namely (1) transport, (2) metabolic process, (3) biological process, (4) photosynthesis, (5) oxidation-reduction, (6) transcription, (7) translation, (8) binding, (9) protein phosphorylation, (10) protein folding, (11) ubiquitin-dependent protein catabolic process, (12) RNA processing, (13) negative regulation of protein, (14) methylation, and (15) mitosis. RT-PCR analysis of 10 selected highly significant genes (p ≤ 0.01) confirmed the microarray results. Overall, we show that candidate genes induced in leaves of E1-3 were implicated in tuberization processes such as transport, carbohydrate metabolism, phytohormones, and transcription/translation/binding functions. Hence, our results provide an insight into the candidate genes induced in leaf tissues during tuberization in E1-3.
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Jagesh Kumar
JK
Tiwari
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Sapna
S
Devi
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
S
S
Sundaresha
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Poonam
P
Chandel
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Nilofer
N
Ali
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Brajesh
B
Singh
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Vinay
V
Bhardwaj
Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.|Central Potato Research Institute, Shimla, Himachal Pradesh - 171 001, India.
Bir Pal
BP
Singh
D050260:Carbohydrate Metabolism / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D046228:Microarray Analysis / Q000379:methods*; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D011198:Solanum tuberosum / Q000235:genetics*
D018749:RNA, Plant
Solanum etuberosum; Solanum tuberosum; expression génique; gene expression; hybride somatique de pomme de terre; microarray; potato somatic hybrid; puce à ADN; tuberization; tubérisation