[Over-expression of augmenter of liver regeneration promotes proliferation and suppresses hydrogen peroxide-induced apoptosis in LO2 cells].
OBJECTIVE
To investigate the effects of over-expression of 23 kDa augmenter of liver regeneration (ALR) on cell proliferation and apoptosis in the normal human hepatic cell line LO2.


METHODS
The recombinant plasmid expressing 23 kDa ALR (pcDNA6/23 kDa ALR) was constructed and transfected into LO2 cells with MegaTran 1.0 transfection reagent. The expressions of ALR mRNA and protein in LO2 cells were detected by real-time quantitative PCR and Western blotting, respectively; MTS assay was used to detect the cell proliferation of LO2 cells; cell cycle and apoptosis of LO2 cells were measured by flow cytometry.


RESULTS
The recombinant expression plasmid pcDNA6/23 kDa ALR was constructed successfully, and the expression of the target protein 23 kDa ALR increased significantly in the transfected cells. Compared with pcDNA6-myc/HisA group, the transient transfection of pcDNA6/23 kDa ALR into LO2 cells promoted cell proliferation and inhibited cell apoptosis induced by H2O2, however, no significant differences were detected in G0 phase and S phase.


CONCLUSIONS
The over-expression of 23 kDa ALR in LO2 cells promoted the cell proliferation and enhanced cell resistance to H2O2.
Department of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
Ning
N
Xia
Department of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
Ruyu
R
Yan
Institute for Viral Hepatitis, Chongqing Medical University, Chongqing 400010, China.
Qi
Q
Liu
Institute for Viral Hepatitis, Chongqing Medical University, Chongqing 400010, China.
Hang
H
Sun
Institute for Viral Hepatitis, Chongqing Medical University, Chongqing 400010, China.
Hui
H
Guo
Department of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
Ling
L
Zhang
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D003579:Cytochrome Reductases / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D050862:Oxidoreductases Acting on Sulfur Group Donors; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016877:Oxidants; D006861:Hydrogen Peroxide; D003579:Cytochrome Reductases; C500897:GFER protein, human; D050862:Oxidoreductases Acting on Sulfur Group Donors