Fabrication of Acrylonitrile-Butadiene-Styrene Nanostructures with Anodic Alumina Oxide Templates, Characterization and Biofilm Development Test for Staphylococcus epidermidis.
Medical devices can be contaminated by microbial biofilm which causes nosocomial infections. One of the strategies for the prevention of such microbial adhesion is to modify the biomaterials by creating micro or nanofeatures on their surface. This study aimed (1) to nanostructure acrylonitrile-butadiene-styrene (ABS), a polymer composing connectors in perfusion devices, using Anodic Alumina Oxide templates, and to control the reproducibility of this process; (2) to characterize the physico-chemical properties of the nanostructured surfaces such as wettability using captive-bubble contact angle measurement technique; (3) to test the impact of nanostructures on Staphylococcus epidermidis biofilm development. Fabrication of Anodic Alumina Oxide molds was realized by double anodization in oxalic acid. This process was reproducible. The obtained molds present hexagonally arranged 50 nm diameter pores, with a 100 nm interpore distance and a length of 100 nm. Acrylonitrile-butadiene-styrene nanostructures were successfully prepared using a polymer solution and two melt wetting methods. For all methods, the nanopicots were obtained but inside each sample their length was different. One method was selected essentially for industrial purposes and for better reproducibility results. The flat ABS surface presents a slightly hydrophilic character, which remains roughly unchanged after nanostructuration, the increasing apparent wettability observed in that case being explained by roughness effects. Also, the nanostructuration of the polymer surface does not induce any significant effect on Staphylococcus epidermidis adhesion.
Clermont Université, Université d'Auvergne, C-BIOSENSS, EA 4676, BP 10448, F-63000 Clermont-Ferrand, France; Clermont Université, Université Blaise Pascal et Université d'Auvergne, LMGE, UMR CNRS 6023, F-63000 Clermont-Ferrand, France.
Camille
C
Desrousseaux
Clermont Université, Université d'Auvergne, C-BIOSENSS, EA 4676, BP 10448, F-63000 Clermont-Ferrand, France.
Régis
R
Cueff
Clermont Université, Université Blaise Pascal et Université d'Auvergne, LMGE, UMR CNRS 6023, F-63000 Clermont-Ferrand, France; CHU Clermont-Ferrand, Service d'Hygiène Hospitalière, F-63003 Clermont-Ferrand, France.
Claire
C
Aumeran
Clermont Université, Université d'Auvergne, CIDAM, EA 4678, BP 10448, F-63000 Clermont-Ferrand, France.
Ghislain
G
Garrait
Clermont Université, Université d'Auvergne, C-BIOSENSS, EA 4676, BP 10448, F-63000 Clermont-Ferrand, France.
Bénédicte
B
Mailhot-Jensen
Clermont Université, Université Blaise Pascal et Université d'Auvergne, LMGE, UMR CNRS 6023, F-63000 Clermont-Ferrand, France; CHU Clermont-Ferrand, Service d'Hygiène Hospitalière, F-63003 Clermont-Ferrand, France.
Ousmane
O
Traoré
Clermont Université, Université d'Auvergne, C-BIOSENSS, EA 4676, BP 10448, F-63000 Clermont-Ferrand, France; CHU Clermont-Ferrand, Service Pharmacie, F-63003 Clermont-Ferrand, France.
Valérie
V
Sautou
D000181:Acrylonitrile / Q000737:chemistry*; D000537:Aluminum Oxide / Q000737:chemistry*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002070:Butadienes / Q000737:chemistry*; D057785:Catheters / Q000382:microbiology; D004566:Electrodes; D058608:Nanopores; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology*; D020058:Styrene / Q000737:chemistry*; D013696:Temperature
D002070:Butadienes; D011108:Polymers; D020058:Styrene; C031763:1,3-butadiene; D000537:Aluminum Oxide; D000181:Acrylonitrile