Selection and identification of a bacterial community able to degrade and detoxify m-nitrophenol in continuous biofilm reactors.
Nitroaromatics are widely used for industrial purposes and constitute a group of compounds of environmental concern because of their persistence and toxic properties. Biological processes used for decontamination of nitroaromatic-polluted sources have then attracted worldwide attention. In the present investigation m-nitrophenol (MNP) biodegradation was studied in batch and continuous reactors. A bacterial community able to degrade the compound was first selected from a polluted freshwater stream and the isolates were identified by the analysis of the 16S rRNA gene sequence. The bacterial community was then used in biodegradation assays. Batch experiments were conducted in a 2L aerobic microfermentor at 28 °C and with agitation (200 rpm). The influence of abiotic factors in the biodegradation process in batch reactors, such as initial concentration of the compound and initial pH of the medium, was also studied. Continuous degradation of MNP was performed in an aerobic up-flow fixed-bed biofilm reactor. The biodegradation process was evaluated by determining MNP and ammonium concentrations and chemical oxygen demand (COD). Detoxification was assessed by Vibrio fischeri and Pseudokirchneriella subcapitata toxicity tests. Under batch conditions the bacterial community was able to degrade 0.72 mM of MNP in 32 h, with efficiencies higher than 99.9% and 89.0% of MNP and COD removals respectively and with concomitant release of ammonium. When the initial MNP concentration increased to 1.08 and 1.44 mM MNP the biodegradation process was accomplished in 40 and 44 h, respectively. No biodegradation of the compound was observed at higher concentrations. The community was also able to degrade 0.72 mM of the compound at pH 5, 7 and 9. In the continuous process biodegradation efficiency reached 99.5% and 96.8% of MNP and COD removal respectively. The maximum MNP removal rate was 37.9 gm(-3) day(-1). Toxicity was not detected after the biodegradation process.
Cátedra de Higiene y Sanidad. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: julietagonzalez@ffyb.uba.ar.
Ana J
AJ
González
Cátedra de Higiene y Sanidad. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
María S
MS
Fortunato
Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología, Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
Mariana
M
Papalia
Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología, Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
Marcela
M
Radice
Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología, Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
Gabriel
G
Gutkind
Cátedra de Higiene y Sanidad. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
Anahí
A
Magdaleno
Cátedra de Higiene y Sanidad. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
Alfredo
A
Gallego
Cátedra de Higiene y Sanidad. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
Sonia E
SE
Korol
D048908:Aliivibrio fischeri / Q000187:drug effects; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D000460:Chlorophyta / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D008658:Inactivation, Metabolic; D009596:Nitrophenols / Q000032:analysis* / Q000633:toxicity; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
D009596:Nitrophenols; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C049017:3-nitrophenol
Abiotic factors; Biodegradation; Continuous biofilm reactors; Detoxification; m-Nitrophenol