Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds.
This work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu(2+) or Ca(2+) (AlgNbgCu, AlgNbgCa, respectively). Two-dimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu(2+) and Ca(2+) are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu(2+) or Ca(2+) were developed with potential applications for preparation of multifunctional scaffolds for BTE.
Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, 956 Junín 6th floor, PC1113, Buenos Aires, Argentina.
J P
JP
Cattalini
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany.
A
A
Hoppe
Department of Materials, Imperial College London, Prince Consort Road, London SW7 2AZ, UK.
F
F
Pishbin
Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany.
J
J
Roether
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany.
A R
AR
Boccaccini
Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, 956 Junín 6th floor, PC1113, Buenos Aires, Argentina National Research Council (CONICET), Buenos Aires, Argentina.
S
S
Lucangioli
Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, 956 Junín 6th floor, PC1113, Buenos Aires, Argentina National Research Council (CONICET), Buenos Aires, Argentina vmourino@ffyb.uba.ar.
V
V
Mouriño
D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000493:pharmacokinetics; D002118:Calcium / Q000737:chemistry / Q000493:pharmacokinetics; D003300:Copper / Q000737:chemistry / Q000493:pharmacokinetics; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D018786:Bone Substitutes; D003300:Copper; D002118:Calcium
alginate; angiogenesis; bioactive glass; bone tissue engineering; metal ion release; nanocomposites