Improved human gingival fibroblast response to titanium implants coated with ultraviolet-irradiated vitamin D precursor and vitamin E.
OBJECTIVE
Ultraviolet (UV)-irradiated 7-dehydrocholesterol (7-DHC) and vitamin E (VitE)-coated titanium (Ti) implants have a beneficial effect on bone cells. Human gingival fibroblasts (HGFs) are the most abundant cells in periodontal tissues and are involved in the wound healing and repair. The objective of this study was to evaluate the response of HGFs to Ti implants coated with UV-irradiated 7-DHC and VitE, for improved soft-tissue integration of dental implants.


METHODS
Ti surfaces were coated with 7-DHC and VitE, irradiated with UV light and incubated for 48 h at 23°C to allow cholecalciferol (D3 ) synthesis from 7-DHC onto the Ti surface. HGFs were cultured on the modified surfaces and the influence of the coating on these cells was evaluated through the analysis of: (i) biocompatibility; (ii) the mRNA levels of genes involved in the composition and turnover of the extracellular matrix, the inflammatory response, periodontal bone resorption and wound healing; and (iii) the levels of MMP-1 and TIMP-1 proteins.


RESULTS
We found a beneficial effect of UV-irradiated 7-DHC:VitE-coated Ti implants on HGFs. Besides being biocompatible with HGFs, the UV-irradiated 7-DHC and VitE coating increased the levels of collagen III α1 and fibronectin mRNAs. and decreased the level of interleukin-8 mRNA. TIMP-1 was increased at both mRNA and protein levels in HGFs cultured on UV-irradiated 7-DHC:VitE-coated Ti implants. Finally, the UV-irradiated 7-DHC and VitE coating decreased the level of RANKL mRNA in HGFs.


CONCLUSIONS
UV-irradiated 7-DHC:VitE-coated Ti implants have a positive effect on HGFs in vitro by reducing the inflammatory response and extracellular matrix breakdown.
Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Pamla, Spain.|Instituto de Investigación Sanitaria de Palma, Palma, Spain.
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Satué
Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Pamla, Spain.|Instituto de Investigación Sanitaria de Palma, Palma, Spain.
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Gómez-Florit
Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Pamla, Spain.|Instituto de Investigación Sanitaria de Palma, Palma, Spain.
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Monjo
Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Pamla, Spain.|Instituto de Investigación Sanitaria de Palma, Palma, Spain.
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Ramis
D000328:Adult; D016301:Alveolar Bone Loss / Q000628:therapy; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D024061:Collagen Type III / Q000032:analysis; D003684:Dehydrocholesterols / Q000494:pharmacology / Q000528:radiation effects; D015921:Dental Implants; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects*; D005353:Fibronectins / Q000032:analysis; D015870:Gene Expression; D005881:Gingiva / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D008875:Middle Aged; D053245:RANK Ligand / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013499:Surface Properties; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays; D014807:Vitamin D / Q000494:pharmacology* / Q000528:radiation effects; D014810:Vitamin E / Q000494:pharmacology* / Q000528:radiation effects; D014945:Wound Healing
C508606:CXCL8 protein, human; D020099:Coated Materials, Biocompatible; D024061:Collagen Type III; D003684:Dehydrocholesterols; D015921:Dental Implants; D005353:Fibronectins; D016209:Interleukin-8; D053245:RANK Ligand; D012333:RNA, Messenger; C560822:TIMP1 protein, human; C505688:TNFSF11 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D014807:Vitamin D; D014810:Vitamin E; C016705:7-dehydrocholesterol; D014025:Titanium; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
7-dehydrocholesterol; dental implant; soft-tissue integration; vitamin D