Biosensors for Cell Analysis.
Biosensors first appeared several decades ago to address the need for monitoring physiological parameters such as oxygen or glucose in biological fluids such as blood. More recently, a new wave of biosensors has emerged in order to provide more nuanced and granular information about the composition and function of living cells. Such biosensors exist at the confluence of technology and medicine and often strive to connect cell phenotype or function to physiological or pathophysiological processes. Our review aims to describe some of the key technological aspects of biosensors being developed for cell analysis. The technological aspects covered in our review include biorecognition elements used for biosensor construction, methods for integrating cells with biosensors, approaches to single-cell analysis, and the use of nanostructured biosensors for cell analysis. Our hope is that the spectrum of possibilities for cell analysis described in this review may pique the interest of biomedical scientists and engineers and may spur new collaborations in the area of using biosensors for cell analysis.
Department of Biomedical Engineering, University of California, Davis, California 95616; email: arevzin@ucdavis.edu.
Qing
Q
Zhou
Department of Biomedical Engineering, University of California, Davis, California 95616; email: arevzin@ucdavis.edu.
Kyungjin
K
Son
Department of Biomedical Engineering, University of California, Davis, California 95616; email: arevzin@ucdavis.edu.
Ying
Y
Liu
Department of Biomedical Engineering, University of California, Davis, California 95616; email: arevzin@ucdavis.edu.
Alexander
A
Revzin
D000818:Animals; D000906:Antibodies; D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008904:Miniaturization; D036103:Nanotechnology; D015341:Nucleic Acid Probes; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance
D000906:Antibodies; D052157:Aptamers, Nucleotide; D015341:Nucleic Acid Probes
biosensors; cytokine detection; microsystems; nanosensors; paracrine interactions; single-cell analysis