Increased exhaled breath condensate 8-isoprostane after a swimming session in competitive swimmers.
OBJECTIVE
This study aimed to evaluate the levels of 8-isoprostane (8-IsoP) in the airways of competitive swimmers at baseline and after a swimming session according to their airway responsiveness.


METHODS
Twenty-three swimmers and six lifeguards had a baseline spirometry and bronchoprovocative challenges. During a second visit, swimmers performed a usual swimming session while lifeguards stayed in the same pool environment for the same time period. Forced expiratory volume in one second (FEV1) was measured before and 5 min after the end of the session. Exhaled breath condensate (EBC) was sampled before and 10 min after the session and EBC 8-IsoP levels were analysed by enzyme immunoassay. Change in EBC 8-IsoP from baseline to post-swimming session was calculated.


RESULTS
We observed no relationships between airway hyper-responsiveness and 8-IsoP values before or after swimming in swimmers. The levels of 8-IsoP were significantly higher after the training session (mean value 2.9, s = 0.5 pg mL(-1)) than at baseline (mean value 1.9, s = 0.4 pg mL(-1)) in swimmers only (p = .012). EBC 8-IsoP levels after the swimming session significantly correlated with the percent change in FEV1 after swimming.


CONCLUSIONS
EBC 8-IsoP levels were increased after training in swimmers but not in lifeguards, suggesting that exercise-induced hyperpnoea in a chlorinated pool environment increases airways oxidative stress.
a Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec , Québec , Canada.
Mathieu C
MC
Morissette
a Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec , Québec , Canada.
Nicolas
N
Murray
a Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec , Québec , Canada.
Julie
J
Turmel
a Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec , Québec , Canada.
Julie
J
Milot
a Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec , Québec , Canada.
Louis-Philippe
LP
Boulet
a Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec , Québec , Canada.|b Faculté des Sciences du Sport et de l'Éducation physique , Université de Lille , Ronchin , France.
Valérie
V
Bougault
D000293:Adolescent; D000328:Adult; D056352:Athletes / Q000706:statistics & numerical data*; D001944:Breath Tests; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D045853:Exhalation / Q000502:physiology; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology; D011795:Surveys and Questionnaires; D013550:Swimming / Q000502:physiology*; D055815:Young Adult
C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost
Oxidative stress; bronchoconstriction; bronchodilation; swimming