Monitoring microbial metabolites using an inductively coupled resonance circuit.
We present a new approach to monitor microbial population dynamics in emulsion droplets via changes in metabolite composition, using an inductively coupled LC resonance circuit. The signal measured by such resonance detector provides information on the magnetic field interaction with the bacterial culture, which is complementary to the information accessible by other detection means, based on electric field interaction, i.e. capacitive or resistive, as well as optical techniques. Several charge-related factors, including pH and ammonia concentrations, were identified as possible contributors to the characteristic of resonance detector profile. The setup enables probing the ionic byproducts of microbial metabolic activity at later stages of cell growth, where conventional optical detection methods have no discriminating power.
Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany.
Daniil
D
Karnaushenko
Institute for Materials Science and Max Bergmann Center of Biomaterials, Dresden University of Technology, Budapesterstr. 27, 01069 Dresden, Germany.
Larysa
L
Baraban
Institute for Materials Science and Max Bergmann Center of Biomaterials, Dresden University of Technology, Budapesterstr. 27, 01069 Dresden, Germany.
Dan
D
Ye
Institute for Materials Science and Max Bergmann Center of Biomaterials, Dresden University of Technology, Budapesterstr. 27, 01069 Dresden, Germany.
Ilke
I
Uguz
Institute for Solid State Research, IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany.
Rafael G
RG
Mendes
Institute for Solid State Research, IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany.
Mark H
MH
Rümmeli
Laboratory of Genetics, Wageningen University, 6708PB, Wageningen, The Netherlands.
J Arjan G M
JA
de Visser
1] Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany [2] Material Systems for Nanoelectronics, Chemnitz University of Technology, Reichenhainer Str. 70, 09107 Chemnitz, Germany [3] Merge Technologies for Multifunctional Lightweight Structures, Chemnitz University of Technology, Reichenhainer Str. 70, 09107 Chemnitz, Germany.
Oliver G
OG
Schmidt
1] Institute for Materials Science and Max Bergmann Center of Biomaterials, Dresden University of Technology, Budapesterstr. 27, 01069 Dresden, Germany [2] Center for Advancing Electronics Dresden (CfAED), Dresden University of Technology, 01062 Dresden, Germany.
Gianaurelio
G
Cuniberti
Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany.
Denys
D
Makarov
D001419:Bacteria / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D056656:Lab-On-A-Chip Devices