Seminal plasma protein profiles of ejaculates obtained by internal artificial vagina and electroejaculation in Brahman bulls.
The present study was conducted to investigate if differences exist in the seminal plasma protein profile from mature Brahman bulls using two methods of semen collection: internal artificial vagina (IAV) and electroejaculation (EEJ). Semen was collected four times from three bulls on the same day and parameters were assessed immediately post-collection. Seminal plasma proteins were evaluated by 2-D fluorescence difference gel electrophoresis and identified by mass spectrometry. Semen volume was greater (P < 0.05) for EEJ (4.6 ± 0.35 mL) than for IAV (1.86 ± 0.24 mL) but sperm concentration was greater in IAV (1505 ± 189 × 10(6) sperm/mL) than in EEJ samples (344 ± 87 × 10(6) sperm/mL). Sperm motility and the percentage of normal sperm were not different between treatments. Total concentration of seminal plasma proteins was greater for samples collected by IAV as compared to EEJ (19.3 ± 0.9 compared with 13.0 ± 1.8 mg/mL, P < 0.05; respectively). Based on 2-D gels, 22 spots had a greater volume (P < 0.05) in gels derived from IAV samples, corresponding to 21 proteins identified as transferrin, albumin, epididymal secretory glutathione peroxidase, among others. Thirty-three spots, corresponding to 26 proteins, had a greater volume (P < 0.05) in gels derived from EEJ samples. These proteins were identified as spermadhesin-1, Bovine Sperm Protin 1, 3 and 5 isoforms, angiogenin-1, alpha-1B-glycoprotein, clusterin, nucleobindin-1, cathepsins, spermadhesin Z13, annexins, among others. Thus, proteins in greater amounts in samples obtained by IAV and EEJ were mainly of epididymal origin and accessory sex glands, respectively.
Federal Institute of Education, Science and Technology, Brazil. Electronic address: joaopaulo.rego@gmail.com.
J P A
JP
Rego
Department of Animal Science, The Federal University of Ceará, Brazil.
A A
AA
Moura
School of Chemistry & Molecular Biosciences, The University of Queensland, Queensland 4072, Australia.
A S
AS
Nouwens
School of Veterinary Science, The University of Queensland, Queensland 4343, Australia.
M R
MR
McGowan
School of Veterinary Science, The University of Queensland, Queensland 4343, Australia.
G B
GB
Boe-Hansen
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004542:Ejaculation / Q000502:physiology*; D004558:Electric Stimulation; D005260:Female; D008297:Male; D008953:Models, Anatomic; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D029589:Seminal Plasma Proteins / Q000502:physiology*; D012726:Sexual Behavior, Animal / Q000502:physiology; D013048:Specimen Handling; D014621:Vagina
D011506:Proteins; D029589:Seminal Plasma Proteins
Bos indicus bulls; Cattle; Proteomics; Semen collection; Sperm