Serum concentrations of canine interleukin-1 receptor antagonist protein in healthy dogs after incubation using an autologous serum processing system.
The objectives of this study were to optimize and validate a canine IL-1RA ELISA using commercially available reagents and to determine the effect of an autologous serum processing system (IRAP II) on IL-1RA concentrations in canine serum. The clinical detection limit of the optimized ELISA was 188.8 to 39,965.6 pg/mL. The observed-to-expected ratio (O:E) for three serial dilutions for four serum samples ranged from 109.6 to 132.2%. The O:E for four serum samples spiked with four concentrations of canine IL-1 RA ranged from 98.7 to 114.3%. Coefficients of variances for intra- and interassay variability ranged from 1.4 to 3.0 and 6.3 to 9.8, respectively. The ELISA was sensitive, linear, accurate, precise, and reproducible. Mean±SD serum concentration of IL-1RA in 12 healthy dogs was 396.6±208.0 pg/mL. There was a significant increase in IL-1RA when blood was incubated in the IRAP II system (15,955.0±6421.0 pg/mL, P<0.0001).
Comparative Orthopedics and Cellular Therapeutics Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, College Station, TX 77843-4474, USA.
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SS
Huggins
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, College Station, TX 77843-4474, USA.
J S
JS
Suchodolski
Comparative Orthopedics and Cellular Therapeutics Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, College Station, TX 77843-4474, USA.
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RN
Bearden
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, College Station, TX 77843-4474, USA.
J M
JM
Steiner
Comparative Orthopedics and Cellular Therapeutics Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, College Station, TX 77843-4474, USA. Electronic address: bsaunders@cvm.tamu.edu.
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WB
Saunders
D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000097:blood*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D044967:Serum / Q000737:chemistry*
D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1
Autologous serum; Canine interleukin-1 receptor antagonist; ELISA; Orthobiologics; Osteoarthritis