Effect of inoculum and sulfide type on simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry and microbial mechanism.
Four reactors were initiated to study the effect of inoculum and sulfide type on the simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry (Ssu-Nir) process. Anaerobic sludge, aerobic sludge, and water were used as inocula, and Na2S and biogas were used as a sulfide substrate, respectively. Additionally, 454 pyrosequencing of the 16S rRNA gene was used to explore the bacterial diversity. The results showed that sulfur-oxidizing bacteria (Thiobacillus, 42.2-84.4 %) were dominant in Ssu-Nir process and led to the excellent performance. Aerobic sludge was more suitable for inoculation of the Ssu-Nir process because it is better for rapidly enriching dominant sulfur-oxidizing bacteria (Thiobacillus, 54.4 %), denitrifying sulfur-oxidizing bacteria (40.0 %) and denitrifiers (23.9 %). Lower S(2-) removal efficiency (72.6 %) and NO3 (-) removal efficiency (<90 %) of the Ssu-Nir process were obtained using biogas as a sulfide substrate than when Na2S was used. For the Ssu-Nir process with biogas as the sulfide substrate, limiting H2S absorption caused a high relative abundance of sulfur-oxidizing bacteria, Thiobacillus (84.8 %) and Thiobacillus sayanicus (39.6 %), which in turn led to low relative abundance of denitrifiers (1.6 %) and denitrifying sulfur-oxidizing bacteria (24.4 %), low NO3 (-) removal efficiency, and eventually poor performance.
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Lan
L
Wang
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Benping
B
Wei
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China. cza0903@163.com.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China. cza0903@163.com.
Ziai
Z
Chen
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.|Southwest Collaborative Innovation Center of Swine for Quality and Safety, Chengdu, 611130, People's Republic of China.
Liangwei
L
Deng
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Li
L
Song
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Shuang
S
Wang
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Dan
D
Zheng
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Yi
Y
Liu
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Xiaodong
X
Pu
Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China.|Laboratory of Development and Application of Rural Renewable Energy, Chengdu, 610041, China.
Yunhong
Y
Zhang
D000693:Anaerobiosis; D000818:Animals; D056804:Biofuels / Q000382:microbiology*; D058448:Biota; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013552:Swine
D056804:Biofuels; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D009584:Nitrogen; D006862:Hydrogen Sulfide
454 pyrosequencing; Bacterial diversity; Biogas; Hydrogen sulfide removal; Nitrogen removal; Swine slurry