Application of Bacillus subtilis to the roots of leafy greens, in the presence of Listeria innocua and Salmonella Newport, induces closure of stomata.
Plant growth-promoting rhizobacterium Bacillus subtilis UD1022 has been shown to trigger an induced systemic response in Arabidopsis thaliana. This interaction causes plant stomata to close, protecting the plant from infection by plant pathogens and thereby increasing crop yield. The purpose of this study was to determine whether UD1022 applied to the roots of plants is able to induce stomata closure in leafy greens as well as influence the persistence of human pathogens (Listeria and Salmonella) on plants. UD1022 induced stomata closure in the presence of human pathogens on both lettuce and spinach 3 h post-inoculation (p<0.0001). Results were confirmed by root inoculation with heat-killed UD1022, which did not induce stomata closure. Presence of UD1022 on lettuce roots significantly reduced the persistence of Listeria on plants after 3 days post-inoculation (p=0.02) but had less of an effect on the persistence of Salmonella. The results of this study indicate that plant growth-promoting rhizobacterium B. subtilis UD1022 may be able to prevent contamination by some human pathogens. This is the first study to investigate the use of plant growth-promoting rhizobacteria to control the persistence of human pathogens on plants.
1 Department of Animal and Food Sciences, University of Delaware , Newark, Delaware.
Sarah M
SM
Markland
1 Department of Animal and Food Sciences, University of Delaware , Newark, Delaware.
Angela M
AM
Ferelli
1 Department of Animal and Food Sciences, University of Delaware , Newark, Delaware.
Shani A
SA
Craighead
2 Department of Plant and Soil Sciences, University of Delaware , Newark, Delaware.|3 Delaware Biotechnology Institute , Newark, Delaware.
Harsh
H
Bais
1 Department of Animal and Food Sciences, University of Delaware , Newark, Delaware.
Kalmia E
KE
Kniel
D001412:Bacillus subtilis / Q000378:metabolism*; D005506:Food Contamination / Q000517:prevention & control; D006801:Humans; D018545:Lactuca / Q000382:microbiology / Q000502:physiology*; D008087:Listeria / Q000254:growth & development; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology; D054046:Plant Stomata / Q000382:microbiology / Q000502:physiology*; D012475:Salmonella / Q000254:growth & development; D018724:Spinacia oleracea / Q000382:microbiology / Q000502:physiology*