Concentric Magnetic Structures for Magnetophoretic Bead Collection, Cell Trapping and Analysis of Cell Morphological Changes Caused by Local Magnetic Forces.
Concentric magnetic structures (ring and square) with domain wall (DW) pinning geometry are designed for biological manipulation. Magnetic beads collection was firstly demonstrated to analyse the local magnetic field generated by DWs and the effective regions to capture magnetic targets of size 1 μm. Primary mouse embryonic fibroblasts (MEFs) are magnetically labeled by internalizing poly (styrene sulfonic acid) stabilized magnetic nanoparticles (PSS-MNPs) and then are selectively trapped by head-to-tail DWs (HH DWs) or tail-to-tail DWs (TT DWs) to be arranged into linear shape or cross shape. The morphologies and the nuclear geometry of the cells growing on two kinds of concentric magnetic structures are shown to be distinctive. The intracellular magnetic forces generated by the local magnetic field of DWs are found to influence the behaviour of cells.
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu City, Taiwan.
Chen-Yu
CY
Huang
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu City, Taiwan.
Zung-Hang
ZH
Wei
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology*; D060526:Magnetic Fields; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
D011137:Polystyrenes; C003321:polystyrene sulfonic acid