Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor.
The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Rd, Suzhou 215123, Jiangsu, P. R. China. chenh@suda.edu.cn lidan@suda.edu.cn.
Zengchao
Z
Tang
Yafei
Y
Luan
Dan
D
Li
Hui
H
Du
David M
DM
Haddleton
Hong
H
Chen
D001665:Binding Sites; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D015849:Plasminogen Inactivators / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013499:Surface Properties; D010959:Tissue Plasminogen Activator / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D008024:Ligands; D015849:Plasminogen Inactivators; D010959:Tissue Plasminogen Activator