Adsorption of Hydrophobin-Protein Mixtures at the Air-Water Interface: The Impact of pH and Electrolyte.
The adsorption of the proteins β-casein, β-lactoglobulin, and hydrophobin, and the protein mixtures of β-casein/hydrophobin and β-lactoglobulin/hydrophobin have been studied at the air-water interface by neutron reflectivity, NR. Changing the solution pH from 7 to 2.6 has relatively little impact on the adsorption of hydrophobin or β-lactoglobulin, but results in a substantial change in the structure of the adsorbed layer of β-casein. In β-lactoglobulin/hydrophobin mixtures, the adsorption is dominated by the hydrophobin adsorption, and is independent of the hydrophobin or β-lactoglobulin concentration and solution pH. At pH 2.6, the adsorption of the β-casein/hydrophobin mixtures is dominated by the hydrophobin adsorption over the range of β-casein concentrations studied. At pH 4 and 7, the adsorption of β-casein/hydrophobin mixtures is dominated by the hydrophobin adsorption at low β-casein concentrations. At higher β-casein concentrations, β-casein is adsorbed onto the surface monolayer of hydrophobin, and some interpenetration between the two proteins occurs. These results illustrate the importance of pH on the intermolecular interactions between the two proteins at the interface. This is further confirmed by the impact of PBS, phosphate buffered saline, buffer and CaCl2 on the coadsorption and surface structure. The results provide an important insight into the adsorption properties of protein mixtures and their application in foam and emulsion stabilization.
Unilever Research and Development Laboratory , Port Sunlight, Quarry Road East, Bebington, Wirral,CH62 4ZD, United Kingdom.
Ian M
IM
Tucker
Unilever Research and Development Laboratory , Port Sunlight, Quarry Road East, Bebington, Wirral,CH62 4ZD, United Kingdom.
Jordan T
JT
Petkov
ISIS, STFC, Rutherford Appleton Laboratory, Chilton, Didcot, OXON OX1 0QX, United Kingdom.|Physical and Theoretical Chemistry Laboratory, Oxford University , South Parks Road, Oxford OX1 3QZ, United Kingdom.
Jeffrey
J
Penfold
Physical and Theoretical Chemistry Laboratory, Oxford University , South Parks Road, Oxford OX1 3QZ, United Kingdom.
Robert K
RK
Thomas
Unilever Research Laboratories , Sharnbrook, Beds MK44 1LQ, United Kingdom.
Andrew R
AR
Cox
Unilever Research Laboratories , Sharnbrook, Beds MK44 1LQ, United Kingdom.
Nick
N
Hedges
D000327:Adsorption; D000388:Air; D004573:Electrolytes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*; D014867:Water
D004573:Electrolytes; D011506:Proteins; D014867:Water