Silver and titanium dioxide nanoparticles alter oxidative/inflammatory response and renin-angiotensin system in brain.
The study was designed to examine the effects of silver AgNPs, 20 nm) and titanium dioxide (Aeroxide(®) P25 TiO2NPs, 21 nm) nanoparticles on brain oxidative stress parameters, its antioxidant potential and brain renin-angiotensin system (RAS) in vivo. The analysis was performed 28 days after single dose injection of TiO2NPs and AgNPs (10 or 5 mg/kg body weight, respectively). The AgNPs, but not TiO2NPs, administration resulted in decreased lipid and cholesterol peroxidation. Antioxidant enzymes gene expression and/or activity were changed differently for TiO2NPs and AgNPs group. The TiO2NPs decreased aromatase gene expression, and glutathione peroxidase and reductase activities. In AgNPs group the sodium dismutase 1 and glutathione reductase mRNA levels were decreased as opposed to their activities. Both NPs altered the expression of brain RAS genes (angiotensinogen, renin, angiotensin I converting enzyme 1 and 2), but only TiO2NPs caused similar changes on protein level. The expression of amyloid beta precursor protein gene was not altered by any kind of injected NPs. The TiO2NPs were more potent modulator of gene expression in the brain than AgNPs, despite the two times lower dosage. These results suggest that AgNPs and TiO2NPs exposure may modulate the brain function, but with different strength.
Laboratory of Molecular Biology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland. Electronic address: agata_krawczynska@wp.pl.
Agata
A
Krawczyńska
Division of Nutrition Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Science, Warsaw University of Life Sciences - SGGW, Nowoursynowska 151C, 02-776 Warsaw, Poland.
Katarzyna
K
Dziendzikowska
Division of Nutrition Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Science, Warsaw University of Life Sciences - SGGW, Nowoursynowska 151C, 02-776 Warsaw, Poland.
Joanna
J
Gromadzka-Ostrowska
Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland; Department of Radiobiology and Immunology, Institute of Biology, Jan Kochanowski University, Świetokrzyska 15, 25-406 Kielce, Poland.
Anna
A
Lankoff
Laboratory of Molecular Biology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.
Andrzej Przemysław
AP
Herman
Division of Nutrition Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Science, Warsaw University of Life Sciences - SGGW, Nowoursynowska 151C, 02-776 Warsaw, Poland.
Michał
M
Oczkowski
Division of Nutrition Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Science, Warsaw University of Life Sciences - SGGW, Nowoursynowska 151C, 02-776 Warsaw, Poland.
Tomasz
T
Królikowski
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, Warsaw 02-776, Poland.
Jacek
J
Wilczak
Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.
Maria
M
Wojewódzka
Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland; Department of Molecular Biology and Translational Research, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland; Department of Medical Biology and Translational Research, Faculty of Medicine, University of Information Technology and Management, Sucharskiego 2, Rzeszów, Poland.
Marcin
M
Kruszewski
D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009443:Neuritis / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D020258:Neurotoxicity Syndromes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010316:Particle Size; D017208:Rats, Wistar; D012084:Renin-Angiotensin System / Q000187:drug effects*; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D018675:Toxicity Tests
D016564:Amyloid beta-Protein Precursor; D009419:Nerve Tissue Proteins; C009495:titanium dioxide; D012834:Silver; D014025:Titanium; D010088:Oxidoreductases
Antioxidant enzymes; Brain; Inflammation; Nanoparticles; Oxidative stress; Renin–angiotensin system