Intensified fractionation of brewery yeast waste for the recovery of invertase using aqueous two-phase systems.
The potential recovery of high-value products from brewery yeast waste confers value to this industrial residue. Aqueous two-phase systems (ATPS) have demonstrated to be an attractive alternative for the primary recovery of biological products and are therefore suitable for the recovery of invertase from this residue. Sixteen different polyethylene glycol (PEG)-potassium phosphate ATPS were tested to evaluate the effects of PEG molecular weight (MW) and tie-line length (TLL) upon the partition behavior of invertase. Concentrations of crude extract from brewery yeast waste were then varied in the systems that presented the best behaviors to intensify the potential recovery of the enzyme. Results show that the use of a PEG MW 400 g mol-1system with a TLL of 45.0% (w/w) resulted in an invertase bottom phase recovery with a purification factor of 29.5 and a recovery yield of up to 66.2% after scaling the system to a total weight of 15.0 g. This represents 15.1 mg of invertase per mL of processed bottom phase. With these results, a single-stage ATPS process for the recovery of invertase is proposed.
Centro de Biotecnología-FEMSA Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, México.
Grecia
G
De León-González
Centro de Biotecnología-FEMSA Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, México.
José
J
González-Valdez
Centro de Biotecnología-FEMSA Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, México.
Karla
K
Mayolo-Deloisa
Centro de Biotecnología-FEMSA Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, México.
Marco
M
Rito-Palomares
D005591:Chemical Fractionation / Q000379:methods*; D007220:Industrial Waste; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification; D014867:Water / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000302:isolation & purification*
D007220:Industrial Waste; D029701:Saccharomyces cerevisiae Proteins; D014867:Water; D043324:beta-Fructofuranosidase
aqueous two-phase systems; invertase; primary recovery; spent yeast