Volatiles as Chemosystematic Markers for Distinguishing Closely Related Species within the Pinus mugo Complex.
Headspace solid-phase microextraction (HS-SPME) coupled to GC/MS analysis was used to identify the constituents of pine-needle volatiles differentiating three closely-related pine species within the Pinus mugo complex, i.e., P. uncinata Ramond ex DC., P. uliginosa G.E.Neumann ex Wimm., and P. mugo Turra. Moreover, chemosystematic markers were proposed for the three analyzed pine species. The major constituents of the pine-needle volatiles were α-pinene (28.4%) and bornyl acetate (10.8%) for P. uncinata, δ-car-3-ene (21.5%) and α-pinene (16.1%) for P. uliginosa, and α-pinene (20%) and δ-car-3-ene (18.1%) for P. mugo. This study is the first report on the application of the composition of pine-needle volatiles for the reliable identification of closely-related pine species within the Pinus mugo complex.
Adam Mickiewicz University in Pozań, Department of Genetics, Umultowska 89, PL-61 - 614 Poznań, (phone: +48-618295862). celinski@amu.edu.pl.
Konrad
K
Celiński
Lodz University of Technology, Institute of General Food Chemistry, Stefanowskiego 4/10, PL-90-924 Łódź
Radosław
R
Bonikowski
Adam Mickiewicz University in Pozań, Department of Genetics, Umultowska 89, PL-61 - 614 Poznań, (phone: +48-618295862).
Aleksandra
A
Wojnicka-Półtorak
Adam Mickiewicz University in Pozań, Department of Genetics, Umultowska 89, PL-61 - 614 Poznań, (phone: +48-618295862).
Ewa
E
Chudzińska
Poznań University of Life Sciences, Faculty of Forestry, Department of Forest Botany, Wojska Polskiego 71D, PL-60-625 Poznań
Tomasz
T
Maliński
D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis*; D001892:Camphanes / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry*; D028223:Pinus / Q000737:chemistry* / Q000145:classification; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis*
D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D001892:Camphanes; D039821:Monoterpenes; D009822:Oils, Volatile; D055549:Volatile Organic Compounds; C071528:bornyl acetate; C030218:3-carene; C005451:alpha-pinene
Chemosystematic markers; GC/MS Analysis; Headspace solid-phase microextraction (HS-SPME); Needle Volatiles; Pinus mugo complex; Pinus uliginosa; Pinus uncinata