Neonatal serotonin reuptake inhibition reduces hypercaloric diet effects on fat mass and hypothalamic gene expression in adult rats.
BACKGROUND
Serotonin (5-HT) is involved in nervous system ontogenesis, and is important for neurotransmission and behavior modulation after the developmental stage. Alterations in 5-HT levels during the early period of life may signal to feeding behavior and hypothalamic genic expression changes in adulthood.


OBJECTIVE
Investigate the effects of hypercaloric diet in adult rats submitted to neonatal serotonin reuptake inhibition on food intake, fat pad mass, plasmatic triglycerides/cholesterol and gene expression of hypothalamic peptides (POMC, NPY) and serotonin receptors (5-HT1B, 5-HT2C).


METHODS
In each litter, 8 pups were divided into two groups: control (C) and fluoxetine (F). From the 1(st) to the 21(st) postnatal day, C pups received sterile saline while F pups received fluoxetine (10mg/kg). From 180 to 215 days, a group of rats from C and F groups were fed hypercaloric diet (CH and FH, 421.4Kcal/100 g) while the rest of animals from C and F groups fed chow diet (CC and FC).


RESULTS
The use of hypercaloric diet was associated with lower accumulation of white adipose tissue in adult rats subjected to neonatal serotonin reuptake inhibition. Adult rats of group FC showed decreased 5-HT2C and neuropeptide Y mRNA expression compared with control chow diet group (CC). After chronic use of a hypercaloric diet, the expression of 5-HT2C was higher in the FH group than the FC group and neuropeptide Y expression decreased in FH related to FC.


CONCLUSIONS
These findings suggest that neonatal serotonin reuptake inhibition is associated with better adaptation to hypercaloric diet in adult rats.
Department of Anatomy-Federal University of Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: galindo.ligia1@gmail.com.
Lígia Cristina Monteiro
LC
Galindo
Master Student in Nutrition, Physical Activity and Phenotypic Plasticity, CAV, Federal University of Pernambuco, 55608-680 VitóriaStoAntão, Brazil. Electronic address: manuella.daluz@gmail.com.
Manuella da Luz Duarte
Mda L
Barros
Department of Physical Education and Sports Science, CAV, Federal University of Pernambuco, 55608-680 VitóriaStoAntão, Brazil. Electronic address: isabeli.lins@gmail.com.
Isabeli Lins
IL
Pinheiro
Department of Anatomy-Federal University of Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: rvcs.fisioterapia@gmail.com.
Ricardo Vinicius de Carvalho
RV
Santana
Department of Physical Education and Sports Science, CAV, Federal University of Pernambuco, 55608-680 VitóriaStoAntão, Brazil. Electronic address: rhowenajane@gmail.com.
Rhowena Jane Barbosa
RJ
de Matos
Department of Physical Education and Sports Science, CAV, Federal University of Pernambuco, 55608-680 VitóriaStoAntão, Brazil. Electronic address: carolleandro22@gmail.com.
Carol Góis
CG
Leandro
Department of Anatomy-Federal University of Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: sanlopesufpe@gmail.com.
Sandra Lopes
SL
de Souza
Department of Nutrition-Federal University of Pernambuco, 50670-901 Recife, Brazil. Electronic address: manhaesdecastroraul@gmail.com.
Raul Manhães
RM
de Castro
D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D004032:Diet / Q000009:adverse effects; D004435:Eating / Q000187:drug effects; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood
D002784:Cholesterol; D005473:Fluoxetine; D009478:Neuropeptide Y; D011333:Pro-Opiomelanocortin; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D014280:Triglycerides
Adipose tissue; Developmental plasticity; Food intake; Hypercaloric diet; Rats; SSRI; Serotonin