Correlation between antibiotic resistance and virulence of Pseudomonas aeruginosa clinical isolates.
OBJECTIVE
Virulent Pseudomonas aeruginosa. is frequently life-threatening and often challenging to treat, and the emergence of multidrug-resistant isolates presents a critical problem for patients. The aim of the study was concerned with molecular analysis of the virulence factors and antimicrobial resistance profile of multidrug-resistant P. aeruginosa (MDRPA).


METHODS
Out of 44 MDRPA isolates, 12 isolates representing different resistance profiles and sources of samples were selected for further molecular studies. Polymerase chain reaction (PCR) approaches were applied to identify the genes implicated in antimicrobial resistance or virulence factors in the selected MDRPA isolates.


RESULTS
Multidrug-resistance (pstS), β-lactamase (IMP7, IMP10, IMP13, and IMP25), and extended spectrum β-lactamase (blaOXA50) genes were detected in all of the selected MDRPA isolates. However, only 4 (33%) MDRPA isolates were positive for the presence of the extended spectrum β-lactamase (blaOXA2) gene. Furthermore, the hemolytic phospholipase C precursor gene (plcH) was detected in all PCR products of the tested MDRPA isolates while the exotoxin A (toxA) gene was absent. Other virulence genes were detected with variable percentage in tested isolates.


CONCLUSIONS
The statistical analysis revealed a significantly positive correlation (r = 0.779, P = 0.002) between virulence factors and antimicrobial resistance marker profiles of the tested MDRPA isolates.
Fatma Ibrahim
FI
Sonbol
Maha Abd El Fattah
MA
Khalil
Abdelfattah Badr
AB
Mohamed
Sameh Samir
SS
Ali
D000704:Analysis of Variance; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics; D014774:Virulence / Q000235:genetics / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
D037521:Virulence Factors; D017855:Transferases (Other Substituted Phosphate Groups); C477519:sphingomyelin synthase, Pseudomonas aeruginosa; D001618:beta-Lactamases