Serum detection of IgG antibodies against Demodex canis by western blot in healthy dogs and dogs with juvenile generalized demodicosis.
The aim of this study was to investigate the presence of canine immunoglobulins (Ig) G against Demodex proteins in the sera of healthy dogs and of dogs with juvenile generalized demodicosis (CanJGD) with or without secondary pyoderma. Demodex mites were collected from dogs with CanJGD. Protein concentration was measured and a western blot technique was performed. Pooled sera from healthy dogs reacted mainly with antigen bands ranging from 55 to 72 kDa. Pooled sera from dogs with CanJGD without secondary pyoderma reacted either with 10 kDa antigen band or 55 to 72 kDa bands. Pooled sera from dogs with CanJGD with secondary pyoderma reacted only with a 10 kDa antigen band. The results of this study suggest that both healthy dogs and dogs with CanJGD develop a humoral response against different proteins of Demodex canis.
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ivanravera@gmail.com.
Ivan
I
Ravera
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Diana
D
Ferreira
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Laia Solano
LS
Gallego
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Mar
M
Bardagí
Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
Lluís
L
Ferrer
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology*; D004285:Dogs; D007074:Immunoglobulin G / Q000097:blood*; D008924:Mite Infestations / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D008925:Mites / Q000276:immunology*; D011711:Pyoderma / Q000276:immunology / Q000662:veterinary*
D007074:Immunoglobulin G
Antibodies; Demodex; Demodex proteins; Demodicosis; Western blot