Sperm quality and selected biochemical parameters of seminal fluid in dogs with benign prostatic hyperplasia.
Benign prostatic hyperplasia (BPH) in dogs is most commonly associated with age and increasing concentrations of dihydrotesterone, a hormone that stimulates growth and secretion of the prostatic epithelial cells. During this process, the biochemical composition of prostatic secretion changes, which can affect the quality of semen and limit the ability of the sperm to contribute to fertilization. Therefore, the present study was conducted to examine possible correlation between BPH and biological quality of semen. The study was performed in 11 sexually mature dogs of various breeds. Animals were divided into two groups: healthy dogs (Group I; n = 5; mean age 4.32; SEM = 1.28) and dogs with BPH (Group II n = 6; mean age 6.16; SEM = 0.65). Semen and prostate secretions were collected and evaluated in this study. Standard semen examinations were conducted in the ejaculates collected; moreover, the extent of apoptosis and DNA defragmentation was determined. The selected biochemical parameters were determined in the prostate secretion. According to the examination results, there were no significant differences in standard semen parameters between the two groups of dogs. Nevertheless, morphological tests of semen in dogs with BPH demonstrated elevated percentages of primary defects in spermatozoa. A significant increase (P = 0.01) in DNA defragmentation of sperm was found in dogs with BPH. Moreover, changes in the biochemical composition of prostate secretion were demonstrated. In dogs with BPH, pH of prostate secretions was greater (P = 0.03), concentrations of cholesterol increased while concentrations of Zn and Cu decreased. The study findings reveal that BPH does not change semen quality in dogs.
Department of Andrology and Biotechnology of Reproduction, Chair of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30 St., 20-612 Lublin, Poland. Electronic address: leszek.krakowski@up.lublin.pl.
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Krakowski
Department of Andrology and Biotechnology of Reproduction, Chair of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30 St., 20-612 Lublin, Poland.
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Wąchocka
Department of Andrology and Biotechnology of Reproduction, Chair of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30 St., 20-612 Lublin, Poland.
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Brodzki
Department of Andrology and Biotechnology of Reproduction, Chair of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30 St., 20-612 Lublin, Poland.
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Wrona
Department of Andrology and Biotechnology of Reproduction, Chair of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30 St., 20-612 Lublin, Poland.
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Piech
Department of Andrology and Biotechnology of Reproduction, Chair of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30 St., 20-612 Lublin, Poland.
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Wawron
Department of Toxicology and Environmental Protection, University of Life Sciences in Lublin, Akademicka 12 St., 20-033 Lublin, Poland.
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Chałabis-Mazurek
D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D008297:Male; D011470:Prostatic Hyperplasia / Q000378:metabolism / Q000662:veterinary*; D012661:Semen / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary*; D013094:Spermatozoa
Apoptosis; DNA; Hyperplasia; Prostate; Semen quality