Photocatalytic oxidation of TMB with the double stranded DNA-SYBR Green I complex for label-free and universal colorimetric bioassay.
We report here the newly discovered photocatalytic activity of the dsDNA-SYBR Green I (SG) complex, which can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under light irradiation corresponding to the excitation of the dsDNA-SG complex. The most appealing feature of the photocatalytic system here is that it can be obtained using random DNA sequences that can form a duplex. Considering the universality of the photooxidase, a label-free and universal platform was proposed for highly sensitive visual bioassays.
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China.
Xinfeng
X
Zhang
Chengpeng
C
Huang
Shuxia
S
Xu
Junbo
J
Chen
Ying
Y
Zeng
Peng
P
Wu
Xiandeng
X
Hou
D052160:Benzothiazoles; D001681:Biological Assay / Q000379:methods*; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D003959:Diamines; D009930:Organic Chemicals / Q000737:chemistry*; D055668:Photochemical Processes; D011804:Quinolines
D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA