Determination of arbutin and bergenin in Bergeniae Rhizoma by capillary electrophoresis with a carbon nanotube-epoxy composite electrode.
This report describes the fabrication and the application of a novel carbon nanotube (CNT)-epoxy composite electrode as a sensitive amperometric detector for the capillary electrophoresis (CE). The composite electrode was fabricated on the basis of the in situ polycondensation of a mixture of CNTs and 1,2-ethanediamine-containing bisphenol A epoxy resin in the inner bore of a piece of fused silica capillary under heat. It was coupled with CE for the separation and detection of arbutin and bergenin in Bergeniae Rhizoma, a traditional Chinese medicine, to demonstrate its feasibility and performance. The two phenolic constituents were well separated within 10min in a 45cm capillary length at a separation voltage of 12kV using a 50mM borate buffer (pH 9.2). The CNT-based detector offered higher sensitivity, significantly lower operating potential, satisfactory resistance to surface fouling, and lower expense of operation, indicating great promise for a wide range of analytical applications. It showed long-term stability and reproducibility with relative standard deviations of less than 5% for the peak current (n=15).
School of Pharmacy, 826 Zhangheng Road, Shanghai 201203, China.
Luyan
L
Zhang
School of Pharmacy, 826 Zhangheng Road, Shanghai 201203, China.
Wei
W
Zhang
School of Pharmacy, 826 Zhangheng Road, Shanghai 201203, China. Electronic address: gangchen@fudan.edu.cn.
Gang
G
Chen
D001104:Arbutin / Q000032:analysis*; D001559:Benzhydryl Compounds / Q000737:chemistry; D001578:Benzopyrans / Q000276:immunology*; D002021:Buffers; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000592:standards; D004853:Epoxy Resins / Q000737:chemistry*; D004867:Equipment Design; D005029:Ethylenediamines / Q000737:chemistry; D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots; D015203:Reproducibility of Results; D031995:Saxifragaceae / Q000737:chemistry*; D013499:Surface Properties; D000098826:Bisphenol A Compounds
D001104:Arbutin; D001559:Benzhydryl Compounds; D001578:Benzopyrans; D002021:Buffers; D004853:Epoxy Resins; D005029:Ethylenediamines; D037742:Nanotubes, Carbon; D010636:Phenols; D010936:Plant Extracts; C031234:ethylenediamine; C006741:bergenin; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Amperometric detection; Arbutin; Bergenin; Capillary electrophoresis; Carbon nanotube; Epoxy