Ubiquitous Structural Signaling in Bacterial Phytochromes.
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Alexander
A
Björling
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Oskar
O
Berntsson
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.|Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä , 40014 Jyväskylä, Finland.
Heikki
H
Takala
Department of Biology, Northeastern Illinois University , 5500 North St. Louis Avenue, Chicago, Illinois 60625, United States.
Kevin D
KD
Gallagher
Department of Biology, Northeastern Illinois University , 5500 North St. Louis Avenue, Chicago, Illinois 60625, United States.
Hardik
H
Patel
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Emil
E
Gustavsson
Department of Biology, Northeastern Illinois University , 5500 North St. Louis Avenue, Chicago, Illinois 60625, United States.
Rachael
R
St Peter
Department of Biology, Northeastern Illinois University , 5500 North St. Louis Avenue, Chicago, Illinois 60625, United States.
Phu
P
Duong
Department of Biology, Northeastern Illinois University , 5500 North St. Louis Avenue, Chicago, Illinois 60625, United States.
Angela
A
Nugent
Botanical Institute, Karlsruhe Institute of Technology KIT , Kaiserstr. 2, 76131 Karlsruhe, Germany.
Fan
F
Zhang
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.|Centre for Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University , Melbourne, Victoria 3086, Australia.
Peter
P
Berntsen
MAX IV Laboratory, Lund University , P.O. Box 118, Lund SE-221 00, Sweden.
Roberto
R
Appio
Paul Scherrer Institut , 5232 Villigen PSI, Switzerland.
Ivan
I
Rajkovic
Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä , 40014 Jyväskylä, Finland.
Heli
H
Lehtivuori
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Matthijs R
MR
Panman
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Maria
M
Hoernke
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Stephan
S
Niebling
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Rajiv
R
Harimoorthy
Botanical Institute, Karlsruhe Institute of Technology KIT , Kaiserstr. 2, 76131 Karlsruhe, Germany.
Tilman
T
Lamparter
Department of Biology, Northeastern Illinois University , 5500 North St. Louis Avenue, Chicago, Illinois 60625, United States.
Emina A
EA
Stojković
Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä , 40014 Jyväskylä, Finland.
Janne A
JA
Ihalainen
Department of Chemistry and Molecular Biology, University of Gothenburg , Box 462, 40530 Gothenburg, Sweden.
Sebastian
S
Westenhoff
D001419:Bacteria / Q000737:chemistry*; D010834:Phytochrome / Q000737:chemistry*; D015398:Signal Transduction
D010834:Phytochrome
X-ray scattering; phytochromes; protein dynamics; signal transduction