Effects of a synovial flap and gelatin/β-tricalcium phosphate sponges loaded with mesenchymal stem cells, bone morphogenetic protein-2, and platelet rich plasma on equine osteochondral defects.
This study aimed to evaluate the efficacy of a synovial flap and gelatin/β-tricalcium phosphate (GT) sponge loaded with mesenchymal stem cells (MSCs), bone morphogenetic protein-2 (BMP-2), and platelet rich plasma (PRP) for repairing of osteochondral defects in horses. Osteochondral defects were created on the medial condyle of both femurs (n=5). In the test group, a GT sponge loaded with MSCs, BMP-2, and PRP (GT/MSCs/BMP-2/PRP) was inserted into the defect and then covered with a synovial flap. In the control group, the defect was treated only with the GT/MSCs/BMP-2/PRP. The test group showed significantly higher macroscopic scores than the control group. In addition, hyaline cartilaginous tissue was detected in the test group in areas larger than those in the control group. This study demonstrated that the combination of a synovial flap and GT sponge loaded with MSCs, BMP-2, and PRP promoted osteochondral regeneration in an equine model.
Department of Veterinary Clinical Sciences, Jeju National University, Jeju-City, Jejudo 690-756, Republic of Korea.
Jong-Pil
JP
Seo
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan.
Yoshinori
Y
Kambayashi
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan.
Megumi
M
Itho
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan.
Shingo
S
Haneda
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan.
Kazutaka
K
Yamada
Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan.
Hidefumi
H
Furuoka
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
Yasuhiko
Y
Tabata
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan. Electronic address: naoki@obihiro.ac.jp.
Naoki
N
Sasaki
D000818:Animals; D001845:Bone Cysts / Q000188:drug therapy* / Q000473:pathology; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D005260:Female; D005781:Gelatin Sponge, Absorbable / Q000627:therapeutic use; D006736:Horses; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D055034:Osteochondrosis / Q000188:drug therapy* / Q000473:pathology; D053657:Platelet-Rich Plasma / Q000502:physiology; D000074402:Synovectomy; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*
D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate
Bone morphogenetic protein; Horse; Mesenchymal stem cell; Platelet rich plasma; Synovial flap; Tissue engineering