Skin lesions on yellowfin tuna Thunnus albacares from Gulf of Mexico outer continental shelf: Morphological, molecular, and histological diagnosis of infection by a capsalid monogenoid.
We characterize lesion-associated capsaline infections on yellowfin tuna, Thunnus albacares, in the Gulf of Mexico by comparing our specimens with published descriptions and museum specimens ascribed to Capsala biparasiticum and its synonyms: vouchers of C. biparasiticum from parasitic copepods; the holotype of Capsala neothunni; and vouchers of Capsala abidjani. Those from parasitic copepods differed by having a small, rounded body, large anterior attachment organs, closely spaced dorsomarginal body sclerites, small testes, and a short and wide testicular field. No morphometric feature in the holotype of C. neothunni ranged outside of that reported for the newly-collected specimens, indicating conspecificity of our specimens. The specimens of C. abidjani differed by having a large anterior attachment organ, few and dendritic testes, and a short, wide testicular field. Large subunit ribosomal DNA (28S) sequences grouped our specimens and Capsala sp. as sister taxa and indicated a phylogenetic affinity of Nasicola klawei. The haptoral attachment site comprised a crater-like depression surrounded by a blackish-colored halo of extensively rugose skin, with abundant pockmarked-like, irregularly-shaped oblong or semi-circular epidermal pits surrounding these attachment sites. Histology confirmed extensive folding of epidermis and underlying stratum laxum, likely epidermal hyperplasia, foci of weak cell-to-cell adhesions among apical malpighian cells as well as that between stratum germinativum and stratum laxum, myriad goblet cells in epidermis, rodlet cells in apical layer of epidermis, and lymphocytic infiltrates and melanin in dermis. The present study comprises (i) the first published report of this parasite from yellowfin tuna captured in the Gulf of Mexico-NW Atlantic Ocean Basin, (ii) confirmation of its infection on the skin (rather than on a parasitic copepod), (iii) the first molecular data for this capsaline, and (iv) the first observations of histopathological changes associated with a capsalid infection on a wild-caught epipelagic fish.
Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences (SFAAS), Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA; Auburn University Center for Aquatic Surveillance and Health, SFAAS, CASIC 559 Devall Drive, Auburn AL 36832, USA. Electronic address: ash.bullard@auburn.edu.
Stephen A
SA
Bullard
Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences (SFAAS), Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA.
Matthew R
MR
Womble
Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences (SFAAS), Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA.
Margaret K
MK
Maynard
Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences (SFAAS), Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA.
Raphael
R
Orélis-Ribeiro
Aquatic Microbiology Laboratory, SFAAS, Auburn University, CASIC 559 Devall Drive, Auburn AL 36832, USA.
Cova R
CR
Arias
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D005393:Fish Diseases / Q000469:parasitology*; D060851:Gulf of America; D006790:Host-Parasite Interactions / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D010986:Platyhelminths / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D014413:Tuna / Q000469:parasitology*
D012339:RNA, Ribosomal, 28S
28S; Gulf of Mexico; Pathology; Skin lesion; Tuna