In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions.
OBJECTIVE
The objective of the present study was to provide an in-vitro evidence for the potential inhibitory activity of extracts and fractions of Adiantum caudatum Linn. and Celosia argentea Linn. on α-amylase and α-glucosidase enzymes.


METHODS
The plant extracts were prepared, first with cold maceration (70% v/v ethanol) and then by Soxhlation techniques (95% v/v ethanol). Subsequently, the combined extracts were subjected for fractionation. Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of extract and fractions were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measured at 540 and 405 nm using multiplate reader and the percentage of α- amylase and α- glucosidase inhibitory activity and IC₅₀ values of extract and fractions were calculated.


RESULTS
Fraction 2 of A. caudatum and fraction 4 of C. argentea has shown highest α-amylase and α-glucosidase inhibitory potential with IC₅₀ values of 0.241, 0.211 and 0.294, 0.249 mg/ml, respectively, which was comparable with acarbose (0.125 and 0.93 mg/ml). Whereas, extracts and remaining fractions of both the plants have shown lesser activity.


CONCLUSIONS
The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.
Department of Pharmacognosy, KLES College of Pharmacy, Belagavi, Karnataka, India.
Madhusudhan
M
Telagari
Department of Pharmacognosy, KLES College of Pharmacy, Belagavi, Karnataka, India.
Kirankumar
K
Hullatti
D035681:Adiantum / Q000737:chemistry*; D034762:Celosia / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors; D000520:alpha-Glucosidases / Q000187:drug effects
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases
Mayurasikha; type 2 diabetes; α- amylase; α-glucosidase