First complete genome of an Ambidensovirus; Cherax quadricarinatus densovirus, from freshwater crayfish Cherax quadricarinatus.
In 1999, the causative agent of an epizootic in Cherax quadricarinatus was described, and given the provisional name Cherax quadricarinatus parvovirus-like. Sequencing of the 6334 nt genome identified three open-reading frames on the top strand coding NS3 (35.55 kDa), NS1 (67.36 kDa) and NS2 (35.18 kDa) and on the bottom strand a single open reading frame which most likely encodes 4 structural proteins. Motifs characteristic of the Densovirinae were found in the ORFs. Phylogenetic analysis of the amino acids in NS1 places the genome in the genus Ambidensovirus, most closely related to the marine sea star densovirus (75%, E=0.0) and distantly related to Acheta domestica densovirus (44.1%). The virus name is proposed as species Decapod ambidensovirus, variant Cherax quadricarinatus densovirus. This is the first Ambidensovirus to be found in decapod crustaceans and the first of the subfamily Densovirinae to be sequenced from a freshwater crayfish. Cherax quadricarinatus densovirus and sea star densovirus are the first highly related Densovirinae to infect phylogenetically disparate hosts and are thus far, unique among the Densovirinae.
College of Public Health, Medical and Veterinary Sciences, James Cook University, 1 Solander Drive, Townsville, Queensland 4811, Australia. Electronic address: shaun.bochow@my.jcu.edu.au.
Shaun
S
Bochow
College of Public Health, Medical and Veterinary Sciences, James Cook University, 1 Solander Drive, Townsville, Queensland 4811, Australia.
Kelly
K
Condon
College of Public Health, Medical and Veterinary Sciences, James Cook University, 1 Solander Drive, Townsville, Queensland 4811, Australia.
Jennifer
J
Elliman
College of Public Health, Medical and Veterinary Sciences, James Cook University, 1 Solander Drive, Townsville, Queensland 4811, Australia; Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland 4811, Australia. Electronic address: leigh.owens@jcu.edu.au.
Leigh
L
Owens
D000818:Animals; D003400:Astacoidea / Q000821:virology*; D003001:Cloning, Molecular; D019223:Densovirinae / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D010802:Phylogeny; D016133:Polymerase Chain Reaction
Ambidensovirus; Aquaculture; Cherax quadricarinatus; Comparative genomics; Densovirinae; Genome annotation; Parvovirus; Phylogeny