Effect of Cinnamon Oil on Quorum Sensing-Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa.
Quorum sensing (QS) is a system of stimuli and responses in bacterial cells governed by their population density, through which they regulate genes that control virulence factors and biofilm formation. Despite considerable research on QS and the discovery of new antibiotics, QS-controlled biofilm formation by microorganisms in clinical settings has remained a problem because of nascent drug resistance, which requires screening of diverse compounds for anti-QS activities. Cinnamon is a dietary phytochemical that is traditionally used to remedy digestive problems and assorted contagions, which suggests that cinnamon might contain chemicals that can hinder the QS process. To test this hypothesis, the anti-QS activity of cinnamon oil against P. aeruginosa was tested, measured by the inhibition of biofilm formation and other QS-associated phenomena, including virulence factors such as pyocyanin, rhamnolipid, protease, alginate production, and swarming activity. To this end, multiple microscopy analyses, including light, scanning electron and confocal microscopy, revealed the ability of cinnamon oil to inhibit P. aeruginosa PAO1 biofilms and their accompanying extracellular polymeric substances. This work is the first to demonstrate that cinnamon oil can influence various QS-based phenomena in P. aeruginosa PAO1, including biofilm formation.
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India.
Manmohit
M
Kalia
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India.
Vivek Kumar
VK
Yadav
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India.
Pradeep Kumar
PK
Singh
Department of Mathematics, National Institute of Technology, Raipur, India.
Deepmala
D
Sharma
Faculty of Pharmaceutical Sciences, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad, India.
Himanshu
H
Pandey
Department of Chemistry, Motilal Nehru National Institute of Technology, Allahabad, India.
Shahid Suhail
SS
Narvi
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India.
Vishnu
V
Agarwal
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002935:Cinnamomum zeylanicum / Q000737:chemistry*; D007211:Indoles / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010936:Plant Extracts / Q000494:pharmacology; D010938:Plant Oils / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*; D053038:Quorum Sensing / Q000187:drug effects*; D037521:Virulence Factors
D000900:Anti-Bacterial Agents; D007211:Indoles; D010936:Plant Extracts; D010938:Plant Oils; D037521:Virulence Factors; C063155:violacein