Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis.
In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.
Department of Entomology, University of Kentucky , Lexington, Kentucky 40546, United States.|Amity Institute of Biotechnology, Amity University Haryana , Gurgaon 122413, India.
Sumistha
S
Das
Department of Entomology, University of Kentucky , Lexington, Kentucky 40546, United States.|Amity Institute of Biotechnology, Amity University Haryana , Gurgaon 122413, India.
Nitai
N
Debnath
Department of Entomology, University of Kentucky , Lexington, Kentucky 40546, United States.
Yingjun
Y
Cui
Department of Plant and Soil Sciences, University of Kentucky , Lexington, Kentucky 40546, United States.
Jason
J
Unrine
Department of Entomology, University of Kentucky , Lexington, Kentucky 40546, United States.
Subba Reddy
SR
Palli
D000330:Aedes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D002244:Carbon / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D056827:Endosomal Sorting Complexes Required for Transport / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020868:Gene Silencing; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry
D056827:Endosomal Sorting Complexes Required for Transport; D019476:Insect Proteins; D012330:RNA, Double-Stranded; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C405423:steroid receptor RNA activator; D002244:Carbon; D012822:Silicon Dioxide; D048271:Chitosan
CQD; Chitosan; RNAi; SNF7; SRC; dsRNA; mosquito