Physiological and biochemical effects of botanical extract from Piper nigrum Linn (Piperaceae) against the dengue vector Aedes aegypti Liston (Diptera: Culicidae).
The leaves of Piper nigrum L. (Piperaceae) were evaluated for chemical constituents and mosquito larvicidal activity against the larvae of Aedes aegypti. GC and GC-MS analyses revealed that the crude extracts contain 16 compounds. Thymol (20.77%) and ç-elemene (10.42%) were identified as the major constituents followed by cyclohexene, 4-ethenyl-4-methyl-3-(1-methylethenyl)-1-(1 methylethyl)-, (3R-trans) (7.58%), 4,6-octadienoic acid, 2-acetyl-2-methyl-, ethyl ester (6.98), 2(3H)-furanone, 3,4-bis(1,3-benzodioxol-5-ylmethyl) dihydro-, (3R-trans) (6.95%), 1-naphthalenol, 1,2,3,4,4a,7,8,8a-octahydro-1,6-dimethyl-4-(1-methylethyl)-, [1R-(1à,4á,4aá,8aá)]-(Cedreanol) (5.30%), trans-2-undecen-1-ol (4.48%), phytol (4.22%), 1,6-cyclodecadiene, 1-methyl-5-methylene-8-(1-methylethyl)-,[s-(E,E)] (3.78%) and 2,6-dimethyl-3,5,7-octatriene-2-ol, Z,Z (2.39%). Larval mortality was observed after 3 h of exposure period. The crude extract showed remarkable larvicidal activity against Ae. aegypti (LC50 = 34.97). The larvae of Ae. aegypti exposed to the P. nigrum, significantly reduced the activities of α- and β-carboxylesterases and superdioxide. Further, P. nigrum extract was severely affecting the mosquito gut cellular organelles. Based on the results, the chemical constituents of crude extracts of P. nigrum can be considered as a new source of larvicide for the control of Ae. aegypti.
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Jalasteen
J
Lija-Escaline
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India. senthil@msuniv.ac.in.
Sengottayan
S
Senthil-Nathan
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Annamalai
A
Thanigaivel
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Venkatraman
V
Pradeepa
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Prabhakaran
P
Vasantha-Srinivasan
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Athirstam
A
Ponsankar
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Edward Sam
ES
Edwin
Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India.
Selvaraj
S
Selin-Rani
Department of Plant Protection, Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, Egypt.|Department of Botany and Microbiology, College of Science, King Saud University, PO Box: 2455, Riyadh, 11451, Saudi Arabia.
Ahmed
A
Abdel-Megeed
D000330:Aedes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D003715:Dengue / Q000635:transmission* / Q000821:virology; D007303:Insect Vectors / Q000187:drug effects* / Q000502:physiology; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D029222:Piper nigrum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
D007306:Insecticides; D010936:Plant Extracts
Black pepper; Carboxylesterase; Dengue vector; GC-MS; Histopathology; Larvicide; Superoxide