19(4→3)-abeo-abietane diterpenoids from Scrophularia dentata Royle ex Benth.
Five 19(4→3)-abeo-abietane diterpenoids, scrodentoids A-E (1-5), were isolated from the whole plant of Scrophularia dentata. Planar structures of scrodentoids A-E were elucidated mainly by using 1D, 2D NMR and MS data. The absolute configurations of compounds 1 and 2 were established using X-ray crystallographic analysis. The absolute configurations of other compounds were confirmed using HPLC-UV/CD detection. The immunosuppressive effects of compounds 1-5 were studied using a ConA-induced splenocyte proliferation model. These compounds significantly inhibited ConA-induced splenocyte proliferation, with IC50 values in the range of 3.49-133.86 μM. Compounds 1-5 (IC50>10 μM) showed no discernible cytotoxic activity against B16 or MCF-7 cells.
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Liuqiang
L
Zhang
Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Dawei
D
Zhang
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Qi
Q
Jia
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Rui
R
Wang
Tibetan Traditional Medical College, Lhasa 850000, People's Republic of China.
Gaawe
G
Dorje
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Zhili
Z
Zhao
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Fujiang
F
Guo
Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China. Electronic address: yangyifu@mail.shcnc.ac.cn.
Yifu
Y
Yang
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China. Electronic address: ymlius@163.com.
Yiming
Y
Li
D045784:Abietanes / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D061986:MCF-7 Cells; D008546:Melanoma, Experimental; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D032266:Scrophularia / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D007166:Immunosuppressive Agents; D010936:Plant Extracts
Artefact; Immunosuppressive effects; Scrodentoid