Excitation Spectra and Stokes Shift Measurements of Single Organic Dyes at Room Temperature.
We report measurements of excitation and emission spectra of single, polymer-embedded, perylene dye molecules at room temperature. From these measurements, we can derive the Stokes shift for each single molecule. We determined the distribution of excitation and emission peak energies and, thus, the distribution of single molecule Stokes shifts. Single molecule Stokes shifts have not been recorded to date, and the Stokes shift has often been assumed to be constant in single molecule studies. Our data show that the observed spectral heterogeneity in single molecule emission originates not only from synchronous energetic shifts of the excitation and the emission spectra but also from variations in the Stokes shift, speaking against the assumption of constant Stokes shift.
†Nanobiophysics, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Martijn H W
MH
Stopel
†Nanobiophysics, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Christian
C
Blum
†Nanobiophysics, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.|‡Nanobiophysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.|§FOM Institute AMOLF, 104 Science Park, 1098 XG Amsterdam, The Netherlands.
Vinod
V
Subramaniam
Excitation; Fluorescence; Perylene; Photophysics; Single Molecule; Spectral heterogeneity; Spectroscopy