Combined therapeutic efficacy of carvacrol and X-radiation against 1,2-dimethyl hydrazine-induced experimental rat colon carcinogenesis.
Colon cancer is one of the most commonly diagnosed cancers, and is a major cause of cancer morbidity and mortality worldwide. The objective of the present study is to evaluate the combined therapeutic efficacy of carvacrol (CVC) and X-radiation against 1,2-dimethylhydrazine-induced colon cancer. Male albino Wistar rats were randomly divided into six groups. Group 1 served as control; group 2 received 40 mg/kg b.wt of CVC orally everyday throughout the experimental period (32 weeks); groups 3-6 received subcutaneous injections of DMH (20 mg/kg b.wt), once a week for the first 15 weeks; group 4 received a single dose of X-radiation at the 31st week; group 5 received CVC (40 mg/kg b.wt) two days after the last injection of DMH and continued everyday till the end of the experimental period; group 6 received CVC as in group 5 and radiation as in group 4. DMH-treated rats showed increased incidence of aberrant crypt foci (ACF), dysplastic aberrant crypt foci (DACF), mast cell number, argyrophilic nucleolar organizer regions; elevated activities of phase I enzymes, decreased activities of phase II enzymes, decreased mucin content and altered colonic and liver histology as compared to control rats. Though the individual treatments with CVC and X-radiation to DMH-treated rats reversed the above changes, the combined treatment with both CVC and X-radiation showed a marked effect. Our findings emphasize the potential role of combined therapeutic effect of CVC and X-radiation against DMH-induced colon carcinogenesis.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India.
Sivaranjani
S
Arivalagan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India.
Nisha Susan
NS
Thomas
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India.
Balaji
B
Chandrasekaran
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India.
Vijay
V
Mani
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India.
Aktarul Islam
AI
Siddique
Medical Physics Division, Dr. Kamakshi Memorial Hospital, No: 1, Radial Road, Pallikaranai, Chennai, Tamil Nadu, 600 100, India.
Thayalan
T
Kuppsamy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India. nalininam@yahoo.com.
Nalini
N
Namasivayam
D019813:1,2-Dimethylhydrazine; D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D059248:Chemoradiotherapy; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000081007:Cymenes; D004334:Drug Administration Schedule; D004798:Enzymes / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000528:radiation effects; D050216:Metabolic Detoxication, Phase I; D050217:Metabolic Detoxication, Phase II; D039821:Monoterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D009077:Mucins / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D011829:Radiation Dosage; D017208:Rats, Wistar; D013997:Time Factors
D034961:Antigens, Nuclear; D000970:Antineoplastic Agents; D000081007:Cymenes; D004798:Enzymes; D039821:Monoterpenes; D009077:Mucins; C051963:nucleolar organizer region associated proteins; C073316:carvacrol; D019813:1,2-Dimethylhydrazine
Aberrant crypt foci; Carvacrol; Colon cancer; Mast cell; Mucin; X-radiation