[Inhibition of proliferation and migration of human umbilical vein endothelial cells by knockdown of cylindromatosis (CYLD) gene].
OBJECTIVE
To down-regulate the expression of cylindromatosis (CYLD) gene by shRNA and investigate its effect on proliferation, migration and cell signal pathways of human umbilical vein endothelial cells (HUVECs).


METHODS
HUVECs were infected by adenoviruses of CYLD shRNA (experiment group) and GFP (control group), respectively. The proliferation of HUVECs was detected by CCK-8 assay; the cell migration was assessed by a wound healing assay; the related cell signal pathways were analyzed by Western blotting.


RESULTS
CCK-8 showed that the proliferation of the CYLD shRNA-infected HUVECs were significantly lower than that of the control group at 1, 2, 3, 4, 5 days after infection. The wound healing assay revealed that the migration rate of the CYLD shRNA-infected HUVECs was significantly reduced compared with the control HUVECs at 48 hours. Western blotting indicated that the phosphorylation of protein kinase B (AKT) and glycogen synthase kinase 3α/β (GSK3α/β) significantly decreased in the CYLD shRNA-infected HUVECs compared with the control HUVECs.


CONCLUSIONS
Inhibiting the expression of CYLD gene can suppress the proliferation and migration of HUVECs, which may be attributed to the inhibition of the AKT/GSK3α/β pathway.
Northwest University, Xi'an 710069; Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Xiaolin
X
Wang
Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Jinqing
J
Li
Northwest University, Xi'an 710069, China.
Liwen
L
Li
Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Xueyong
X
Li
D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D000074749:Deubiquitinating Enzyme CYLD; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
D025521:Tumor Suppressor Proteins; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3; C448021:glycogen synthase kinase 3 alpha; C409984:CYLD protein, human; D000074749:Deubiquitinating Enzyme CYLD