Mechanisms of Intracellular Calcium Homeostasis in MC3T3-E1 Cells and Bone Tissues of Sprague-Dawley Rats Exposed to Fluoride.
Calcium homeostasis of osteoblasts (OBs) has an important role in the physiology and pathology of bone tissue. In order to study the mechanisms of intracellular calcium homeostasis, MC3T3-E1 cells and Sprague-Dawley rats were treated with different concentrations of fluoride. Then, we examined intracellular-free calcium ion ([Ca(2+)]i) in MC3T3-E1 cells as well as mRNA and protein levels of Cav1.2, the main subunit of L-type voltage-dependent calcium channels (VDCCs), Na(+)/Ca(2+) exchange carriers (NCS), and plasma membrane Ca(2+)-ATPase (PMCA), inositol 1,4,5-trisphosphate receptor (IP3R) channels, sarco/endoplasmic reticulum calcium ATPase 2b (SERCA2b)/ATP2A2 in vitro, and rat bone tissues in vivo. Our results showed that [Ca(2+)]i of fluoride-treated OBs increased in a concentration-dependent manner with an increase in the concentration of fluoride. We also found that the low dose of fluoride led to high expression levels of Cav1.2, NCS-1, and PMCA and low expression levels of IP3R and SERCA2b/ATP2A2, while the high dose of fluoride induced an increase in SERCA2b/ATP2A2 levels and decrease in Cav1.2, PMCA, NCS-1, and IP3R levels. These results demonstrate that calcium channels and calcium pumps of plasma and endoplasmic reticulum (ER) membranes keep intracellular calcium homeostasis by regulating Cav1.2, NCS-1, PMCA, IP3R, and SERCA2b/ATP2A2 expression.
The Second Hospital Norman Bethune of Jilin University, Changchun, 130041, People's Republic of China.
Xiao-qin
XQ
Duan
The Second Hospital Norman Bethune of Jilin University, Changchun, 130041, People's Republic of China.
Yan-hui
YH
Li
Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun, 130021, People's Republic of China.
Xiu-yun
XY
Zhang
Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun, 130021, People's Republic of China.
Zhi-tao
ZT
Zhao
The First Hospital Norman Bethune of Jilin University, Changchun, 130021, People's Republic of China.
Ying
Y
Wang
The Second Hospital Norman Bethune of Jilin University, Changchun, 130041, People's Republic of China.
Huan
H
Wang
Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun, 130021, People's Republic of China.
Guang-sheng
GS
Li
Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun, 130021, People's Republic of China. jingling85619287@163.com.
Ling
L
Jing
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000494:pharmacology*; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D008297:Male; D051379:Mice; D051580:Neuronal Calcium-Sensor Proteins / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism
C506948:Atp2a2 protein, rat; C514350:CACNA1C protein, mouse; D020746:Calcium Channels, L-Type; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; C101464:frequenin calcium sensor proteins; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; C506947:Atp2a2 protein, mouse; D005459:Fluorides; D002118:Calcium
Calcium channel; Calcium pump; Fluoride; Intracellular calcium homeostasis; Osteoblast (OB)