Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly(ethylene-co-vinyl alcohol).
The immobilization of cells or microorganisms is important for bioseparations, in bioreactors producing cellular metabolites, and as receptors for biosensing. Cell-imprinted polymers (CIPs) have been shown to have cavities with complementary shapes and also high affinities for the template cells or microorganisms. However, the effects of binding to CIPs on gene expression are only beginning to be studied. In this work, the purple bacteria Rhodobacter sphaeroides was employed as a model for the imprinting of microorganisms. R. sphaeroides was first adsorbed on a glass slide as the stamp and then microcontact-imprinted onto poly(ethylene-co-vinyl alcohol), EVAL. The surfaces of the R. sphaeroides-imprinted (RsIPs) and non-imprinted (NIPs) EVAL thin films were examined by Raman spectrometry and scanning electron microscopy. The expression of the nitrogenase (nitrogen fixation, nifH) gene of R. sphaeroides adsorbed on both the RsIPs and NIPs EVAL thin films was also measured by the quantitative reverse transcription polymerase chain reaction (qRT-PCR); cells grown on imprinted polymer showed dramatic differences in gene expression compared to controls.
Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
Mei-Hwa
MH
Lee
Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA.
James L
JL
Thomas
Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
Ming-Huan
MH
Li
Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Ching-Ping
CP
Shih
Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Jeng-Shiung
JS
Jan
Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan. Electronic address: linhy@ntu.edu.tw.
Hung-Yin
HY
Lin
D000327:Adsorption; D015374:Biosensing Techniques; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008838:Microcomputers; D016133:Polymerase Chain Reaction; D011108:Polymers; D011145:Polyvinyls / Q000737:chemistry*; D012242:Rhodobacter sphaeroides / Q000737:chemistry* / Q000235:genetics; D013499:Surface Properties
D011108:Polymers; D011145:Polyvinyls; C016438:ethylenevinylacetate copolymer
Gene expression; Microcontact-imprinting; Nitrogenase; Poly(ethylene-co-vinyl alcohol); Rhodobacter sphaeroides