Binding Patterns of Rotavirus Genotypes P[4], P[6], and P[8] in China with Histo-Blood Group Antigens.
Rotaviruses (RVs) are an important cause of severe gastroenteritis in children. It has been found that RV may recognize the histo-blood group antigens (HBGAs) as ligands or receptors and bind HBGAs in a type-dependent manner. In this study, we investigated the binding specificity of VP8* proteins from human rotaviruses (RV) that are prevalent in China including genotypes P[4], P[6], and P[8]. Through the saliva- and oligosaccharide-based binding assays, we found that the VP8* proteins of P[4] and P[8] RV showed similar reactivity with the Leb and H type 1 antigens, while P[6] RV weakly bound the Leb antigen. These findings may facilitate further research into RV host specificity and vaccine development.
China Railway Construction Corporation, Beijing Tiejian Hospital, Beijing, 100039, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
Xin
X
Ma
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
Dan-di
DD
Li
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
Xiao-Man
XM
Sun
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
Yan-Qing
YQ
Guo
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
Jing-Yao
JY
Xiang
Beijing Railway Center for Disease Control and Prevention, Beijing, 100038, China.
Wei-Huan
WH
Wang
China Railway Construction Corporation, Beijing Tiejian Hospital, Beijing, 100039, China.
Li-Xia
LX
Zhang
China Railway Construction Corporation, Beijing Tiejian Hospital, Beijing, 100039, China.
Qing-Jiu
QJ
Gu
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China.
Zhao-Jun
ZJ
Duan
D000328:Adult; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D001789:Blood Group Antigens / Q000235:genetics / Q000378:metabolism*; D002648:Child; D005260:Female; D005759:Gastroenteritis / Q000097:blood / Q000821:virology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism; D011817:Rabbits; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D012400:Rotavirus Infections / Q000097:blood; D012463:Saliva / Q000378:metabolism / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000378:metabolism
D000956:Antigens, Viral; D001789:Blood Group Antigens; D016601:RNA-Binding Proteins; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus