Combination Growth Factor Therapy via Electrostatically Assembled Wound Dressings Improves Diabetic Ulcer Healing In Vivo.
Chronic skin ulcerations are a common complication of diabetes mellitus, affecting up to one in four diabetic individuals. Despite the prevalence of these wounds, current pharmacologic options for treating them remain limited. Growth factor-based therapies have displayed a mixed ability to drive successful healing, which may be due to nonoptimal delivery strategies. Here, a method for coating commercially available nylon dressings using the layer-by-layer process is described to enable both sustained release and independent control over the release kinetics of vascular endothelial growth factor 165 and platelet-derived growth factor BB. It is shown that the use of strategically spaced diffusion barriers formed spontaneously by disulfide bonds enables independent control over the release rates of incorporated growth factors, and that in vivo these dressings improve several aspects of wound healing in db/db mice.
Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK.|Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Benjamin D
BD
Almquist
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Steven A
SA
Castleberry
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Julia B
JB
Sun
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Alice Y
AY
Lu
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Paula T
PT
Hammond
chronic wound healing; diabetic ulcer; electrostatic assembly; growth factor; layer-by-layer