Long-term boron-deficiency-responsive genes revealed by cDNA-AFLP differ between Citrus sinensis roots and leaves.
Seedlings of Citrus sinensis (L.) Osbeck were supplied with boron (B)-deficient (without H3BO3) or -sufficient (10 μM H3BO3) nutrient solution for 15 weeks. We identified 54 (38) and 38 (45) up (down)-regulated cDNA-AFLP bands (transcript-derived fragments, TDFs) from B-deficient leaves and roots, respectively. These TDFs were mainly involved in protein and amino acid metabolism, carbohydrate and energy metabolism, nucleic acid metabolism, cell transport, signal transduction, and stress response and defense. The majority of the differentially expressed TDFs were isolated only from B-deficient roots or leaves, only seven TDFs with the same GenBank ID were isolated from the both. In addition, ATP biosynthesis-related TDFs were induced in B-deficient roots, but unaffected in B-deficient leaves. Most of the differentially expressed TDFs associated with signal transduction and stress defense were down-regulated in roots, but up-regulated in leaves. TDFs related to protein ubiquitination and proteolysis were induced in B-deficient leaves except for one TDF, while only two down-regulated TDFs associated with ubiquitination were detected in B-deficient roots. Thus, many differences existed in long-term B-deficiency-responsive genes between roots and leaves. In conclusion, our findings provided a global picture of the differential responses occurring in B-deficient roots and leaves and revealed new insight into the different adaptive mechanisms of C. sinensis roots and leaves to B-deficiency at the transcriptional level.
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China ; Institute of Horticultural Plant Physiology, Biochemistry, and Molecular Biology, Fujian Agriculture and Forestry University Fuzhou, China.
Yi-Bin
YB
Lu
Institute of Materia Medica, Fujian Academy of Medical Sciences Fuzhou, China.
Yi-Ping
YP
Qi
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China ; Institute of Horticultural Plant Physiology, Biochemistry, and Molecular Biology, Fujian Agriculture and Forestry University Fuzhou, China.
Lin-Tong
LT
Yang
Department of Horticultural Science, Kyungpook National University Daegu, South Korea.
Jinwook
J
Lee
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China ; Institute of Horticultural Plant Physiology, Biochemistry, and Molecular Biology, Fujian Agriculture and Forestry University Fuzhou, China.
Peng
P
Guo
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China ; Institute of Horticultural Plant Physiology, Biochemistry, and Molecular Biology, Fujian Agriculture and Forestry University Fuzhou, China.
Xin
X
Ye
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China.
Meng-Yang
MY
Jia
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China.
Mei-Li
ML
Li
Department of Resource and Environment, College of Resource and Environmental Science, Fujian Agriculture and Forestry University Fuzhou, China ; Institute of Horticultural Plant Physiology, Biochemistry, and Molecular Biology, Fujian Agriculture and Forestry University Fuzhou, China ; The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University Fuzhou, China.
Li-Song
LS
Chen
Citrus sinensis; boron-deficiency; cDNA-AFLP; leaves; roots; transcript-derived fragment