Effect of molecular weight and concentration of hyaluronan on cell proliferation and osteogenic differentiation in vitro.
Hyaluronan (HA), the simplest glycosaminoglycan and a major component of the extracellular matrix, exists in various tissues. It is involved in some critical biological procedures, including cellular signaling, cell adhesion and proliferation, and cell differentiation. The effect of molecular weight (MW) and concentration of HA on cell proliferation and differentiation was controversial. In this study, we investigated the effect of MW and concentration of HA on the proliferation and osteogenic differentiation of rabbit bone marrow-derived stem cells in vitro. Results showed that high MW HA decreased the cell adhesion rate in a concentration-dependant manner. The cell adhesion rate was decreased by increasing MW of HA. Cell proliferation was significantly enhanced by low MW HA (P < 0.05). The factorial analysis indicated that MW and concentration had an interactive effect on the cell adhesion rate and cell proliferation (P < 0.05). High MW HA increased the mRNA expressions of ALP, RUNX-2 and OCN. The higher the MW was, the higher the mRNA expressions were. The factorial analysis indicated that MW and concentration had an interactive effect on ALP mRNA expression (P < 0.05). HA of higher MW and higher concentration promoted bone formation. These findings provide some useful information in understanding the mechanism underlying the effect of MW and concentration of HA on cell proliferation and differentiation.
State Key Laboratory of Military Stomatology, Department of Oral Implants, School of Stomatology, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: curl-zhao@163.com.
Ningbo
N
Zhao
State Key Laboratory of Military Stomatology, Department of Oral Implants, School of Stomatology, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: 394041230@qq.com.
Xin
X
Wang
State Key Laboratory of Military Stomatology, Department of Oral Implants, School of Stomatology, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: qinlei30@126.com.
Lei
L
Qin
State Key Laboratory of Military Stomatology, Department of Oral Implants, School of Stomatology, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: zhzeguo@163.com.
Zhengze
Z
Guo
State Key Laboratory of Military Stomatology, Department of Oral Implants, School of Stomatology, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address: lidehuafmmu@163.com.
Dehua
D
Li
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D011817:Rabbits
D006820:Hyaluronic Acid
Concentration; Differentiation; Hyaluronan; Molecular weight; Proliferation