Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture.
A well-known problem of cultured primary hepatocytes is their rapid dedifferentiation. During the last years, several strategies to counteract this phenomenon have been developed, of which changing the in vitro environment is the most popular one. However, mimicking the in vivo setting in vitro by adding soluble media additives or the restoration of both cell-cell and cell-extracellular matrix contacts is not sufficient and only delays the dedifferentiation process instead of counteracting it. In this chapter, new strategies to prevent the deterioration of the liver-specific phenotype of primary hepatocytes in culture by targeting the (epi)genetic mechanisms that drive hepatocellular gene expression are described.
Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium.
Jennifer
J
Bolleyn
Joanna
J
Fraczek
Vera
V
Rogiers
Tamara
T
Vanhaecke
D000818:Animals; D054337:Cell Dedifferentiation / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D061251:Primary Cell Culture / Q000379:methods*
D006657:Histones