Optical detection of glucose and glycated hemoglobin using etched fiber Bragg gratings coated with functionalized reduced graphene oxide.
An enhanced optical detection of D-glucose and glycated hemoglobin (HbA1c ) has been established in this study using etched fiber Bragg gratings (eFBG) coated with aminophenylboronic acid (APBA)-functionalized reduced graphene oxide (RGO). The read out, namely the shift in Bragg wavelength (ΔλB ) is highly sensitive to changes that occur due to the adsorption of glucose (or HbA1c ) molecules on the eFBG sensor coated with APBA-RGO complex through a five-membered cyclic ester bond formation between glucose and APBA molecules. A limit of detection of 1 nM is achieved with a linear range of detection from 1 nM to 10 mM in the case of D-glucose detection experiments. For HbA1c , a linear range of detection varying from 86 nM to 0.23 mM is achieved. The observation of only 4 pm (picometer) change in ΔλB even for the 10 mM lactose solution confirms the specificity of the APBA-RGO complex coated eFBG sensors to glucose molecules.
Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
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Sridevi
Department of Physics, Indian Institute of Science, Bangalore, 560012, India.
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Vasu
Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, 560012, India.
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Sampath
Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.|Robert Bosch Centre for Cyber Physical Systems, Indian Institute of Science, Bangalore, 560012, India.
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Asokan
Department of Physics, Indian Institute of Science, Bangalore, 560012, India. asood1951@gmail.com.
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Sood
D005947:Glucose / Q000032:analysis*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry*
D005947:Glucose; D006442:Glycated Hemoglobin; D006108:Graphite; D010087:Oxides
Fiber Bragg grating; HbA1c; aminophenylboronic acid; glucose; reduced graphene oxide