MmoSTI restriction endonuclease, isolated from Morganella morganii infecting a tropical moth, Actias selene, cleaving 5'-|CCNGG-3' sequences.
A type II restriction endonuclease, MmoSTI, from the pathogenic bacterium Morganella morganii infecting a tropical moth, Actias selene, has been detected and biochemically characterized, as a potential etiological differentiation factor. The described REase recognizes interrupted palindromes, i.e., 5'-CCNGG-3' sequences and cleaves DNA leaving 5-nucleotide (nt) long, single-stranded (ss), 5'-cohesive ends, which was determined by three complementary methods: (i) cleavage of custom and standard DNA substrates, (ii) run-off sequencing of cleavage products, and (iii) shotgun cloning and sequencing of bacteriophage lambda (λ) DNA digested with MmoSTI. MmoSTI, the first 5'-CCNGG-3' REase characterized from M. morganii, is a neoschizomer of ScrFI, which cleaves DNA leaving 1-nt long, ss, 5'-cohesive ends. It is a high-frequency cutter and can be isolated from easily cultured bacteria, thus it can potentially serve as a tool for DNA manipulations.
Department of Molecular Biology, Division of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.|Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland.
Marta A
MA
Skowron
Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland.
Joanna
J
Zebrowska
Department of Molecular Biology, Division of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.
Grzegorz
G
Wegrzyn
Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland. piotr.skowron@ug.edu.pl.
Piotr M
PM
Skowron
D000818:Animals; D003001:Cloning, Molecular; D053837:DNA Cleavage; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D055029:Inverted Repeat Sequences; D020613:Morganella morganii / Q000201:enzymology*; D009036:Moths / Q000382:microbiology*
D004262:DNA Restriction Enzymes
Actias selene; Interrupted palindrome; Morganella morganii; Restriction endonuclease