Discovery of urinary biomarkers of whole grain rye intake in free-living subjects using nontargeted LC-MS metabolite profiling.
METHODS
Whole grain (WG) intake is associated with decreased risk of developing colorectal cancer, type 2 diabetes, and cardiovascular disease and its comorbidities. However, the role of specific grains is unclear. Moreover, intake of specific WG is challenging to measure accurately with traditional dietary assessment methods. Our aim was to use nontargeted metabolite profiling to discover specific urinary biomarkers for WG rye to objectively reflect intake under free-living conditions.


RESULTS
WG rye intake was estimated by weighed food records, and 24 h urine collections were analyzed by LC-MS. Multivariate modeling was undertaken by repeated double cross-validated partial least squares regression against reported WG rye intake, which correlated well with multivariate prediction estimates (r = 0.67-0.80, p < 0.001), but not with intakes of WG wheat or oats. Hydroxyhydroxyphenyl acetamide sulfate, 3,5-dihydroxyphenylpropionic acid sulfate, caffeic acid sulfate, and hydroxyphenyl acetamide sulfate were among the 20 features that had the greatest potential as intake biomarkers of WG. In addition, three compounds exhibited MS/MS fragmentation of carnitine structures.


CONCLUSIONS
With this nontargeted approach, we confirmed the specificity of alkylresorcinol metabolites as biomarkers for WG rye intake, but also discovered other compounds that should be evaluated as putative biomarkers in future studies.
Department of Clinical Nutrition, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland.
Kati
K
Hanhineva
Department of Food Science, Uppsala BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Carl
C
Brunius
Department of Food, Nutrition and Dietetics, Uppsala University, Uppsala, Sweden.
Agneta
A
Andersson
Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism, Uppsala Univeristy, Uppsala, Sweden.
Matti
M
Marklund
School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
Risto
R
Juvonen
School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
Pekka
P
Keski-Rahkonen
School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
Seppo
S
Auriola
Department of Food Science, Uppsala BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.|Nutritional Epidemiology Unit, Institute of Environmental Medicine, Karolinska Insitutet, Stockholm, Sweden.
Rikard
R
Landberg
D000081:Acetamides / Q000032:analysis; D000328:Adult; D015415:Biomarkers / Q000652:urine*; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012434:Secale / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D000067348:Whole Grains / Q000378:metabolism*
D000081:Acetamides; D015415:Biomarkers
Biomarker; Metabolite profiling; Metabolomics; Rye; Whole grain