First report of genotype #65 of Toxoplasma gondii in pigs.
The aim of the present study was to isolate and genotype Toxoplasma gondii from pigs slaughtered for human consumption in northeastern Brazil. Indirect immunofluorescence antibody test (IFAT) was used to screen positive pigs. Tissues samples of animals with antibody titers ≥64 were submitted to bioassay in mice. One isolate of T. gondii was obtained, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, using 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1, and APICO), was applied to evaluate the genetic variability. DNA from reference strains was used as a positive control. By means of genetic analysis, genotype ToxoDB #65 was identified, which is considered an atypical strain. This is the first record of genotype #65 in pigs. Thus, further studies in this region are necessary to determine the genetic variability of T. gondii in pigs and possible impact on public health.
Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, PE, Brazil.
Erika Fernanda Torres
EF
Samico-Fernandes
Renata Pimentel Bandeira
RP
de Melo
Pomy
P
de Cássia Peixoto Kim
Jonatas Campos
JC
de Almeida
Luiz Daniel
LD
de Barros
João Luis
JL
Garcia
Jean Carlos Ramos
JC
da Silva
Rinaldo Aparecido
RA
Mota
D000818:Animals; D001938:Brazil; D005838:Genotype; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*