Analysis of phospholipids in bio-oils and fats by hydrophilic interaction liquid chromatography-tandem mass spectrometry.
A new, sensitive and selective liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method was developed for the analysis of Phospholipids (PLs) in bio-oils and fats. This analysis employs hydrophilic interaction liquid chromatography-scheduled multiple reaction monitoring (HILIC-sMRM) with a ZIC-cHILIC column. Eight PL class selective internal standards (homologs) were used for the semi-quantification of 14 PL classes for the first time. More than 400 scheduled MRMs were used for the measurement of PLs with a run time of 34min. The method's performance was evaluated for vegetable oil, animal fat and algae oil. The averaged within-run precision and between-run precision were ≤10% for all of the PL classes that had a direct homologue as an internal standard. The method accuracy was generally within 80-120% for the tested PL analytes in all three sample matrices.
Technology Centre, Kilpilahti, Neste Corporation, P.O. Box 310, FI-06101 Porvoo, Finland. Electronic address: jyrki.viidanoja@neste.com.
Jyrki
J
Viidanoja
D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005223:Fats / Q000737:chemistry*; D057230:Limit of Detection; D009821:Oils / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005223:Fats; D009821:Oils; D010743:Phospholipids
Bio-oil; Electrospray; HILIC; Phospholipid; Tandem mass spectrometry