The Healing Effect of Arnebia Euchroma Ointment versus Silver Sulfadiazine on Burn Wounds in Rat.
BACKGROUND
Burn is still a majordevastating condition in emergency medicine departments among both genders and all age groups in all developed and developing countries, leading to physical, psychological scars and economical burden. The present study aimed to determine the healing effect of topical treatment with Arnebia euchroma on second-degree burn wound in rats.


METHODS
Fifty rats were divided into 4 equal groups receiving the ointment base, normal saline (NS), standard 1% silver sulfadiazine (SSD), and 5% and 10% Arnebia euchroma ointments (AEO). The mean of burn area, percentage of wound contraction, histopathological and bacteriological assessments in the injured area were dtermined during the study.


RESULTS
Average area of wound on the 10(th) day was 10.2±2.3, 8.4±2.6, 12.4±2.5, 5.9±2.2 and 5.7±2 cm(2) for ointment base, NS, 1% SSD, and 5% and 10% AEO, respectively. Wound size was significantly lower in 10% AEO than 1% SSD and control groups on the 10(th) day post-burn injury. On day 11, the percentage of wound contraction in 5% and 10% AEO was 53.9%±14.7% and 55.9±10.5% which was more than 1% SSD (15.3±10.8%). The collagen fibers were well formed and horizontally-oriented in 5% and 10% AEO groups when compared with other groups.


CONCLUSIONS
Arnebia euchroma ointment was an effective treatment for healing of burn wounds in comparison with SSD and can be regarded as an alternative topical treatment for burn wounds.
Traditional and Complementary Medicine Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;
Ebrahim
E
Nasiri
Traditional and Complementary Medicine Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;
Seyed Jalal
SJ
Hosseinimehr
Traditional and Complementary Medicine Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;
Mohammad
M
Azadbakht
Traditional and Complementary Medicine Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;
Jafar
J
Akbari
Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;
Reza
R
Enayati-Fard
Department of Laboratory Medicine, Faculty of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran;
Sohail
S
Azizi
Sana Institute, Mazandaran, Sari, Iran.
Masoud
M
Azadbakht
Arnebia euchroma; Burns; Rat; Silver sulfadiazine; Wound healing