Plasmodium falciparum Hop (PfHop) Interacts with the Hsp70 Chaperone in a Nucleotide-Dependent Fashion and Exhibits Ligand Selectivity.
Heat shock proteins (Hsps) play an important role in the development and pathogenicity of malaria parasites. One of the most prominent functions of Hsps is to facilitate the folding of other proteins. Hsps are thought to play a crucial role when malaria parasites invade their host cells and during their subsequent development in hepatocytes and red blood cells. It is thought that Hsps maintain proteostasis under the unfavourable conditions that malaria parasites encounter in the host environment. Although heat shock protein 70 (Hsp70) is capable of independent folding of some proteins, its functional cooperation with heat shock protein 90 (Hsp90) facilitates folding of some proteins such as kinases and steroid hormone receptors into their fully functional forms. The cooperation of Hsp70 and Hsp90 occurs through an adaptor protein called Hsp70-Hsp90 organising protein (Hop). We previously characterised the Hop protein from Plasmodium falciparum (PfHop). We observed that the protein co-localised with the cytosol-localised chaperones, PfHsp70-1 and PfHsp90 at the blood stages of the malaria parasite. In the current study, we demonstrated that PfHop is a stress-inducible protein. We further explored the direct interaction between PfHop and PfHsp70-1 using far Western and surface plasmon resonance (SPR) analyses. The interaction of the two proteins was further validated by co-immunoprecipitation studies. We observed that PfHop and PfHsp70-1 associate in the absence and presence of either ATP or ADP. However, ADP appears to promote the association of the two proteins better than ATP. In addition, we investigated the specific interaction between PfHop TPR subdomains and PfHsp70-1/ PfHsp90, using a split-GFP approach. This method allowed us to observe that TPR1 and TPR2B subdomains of PfHop bind preferentially to the C-terminus of PfHsp70-1 compared to PfHsp90. Conversely, the TPR2A motif preferentially interacted with the C-terminus of PfHsp90. Finally, we observed that recombinant PfHop occasionally eluted as a protein species of twice its predicted size, suggesting that it may occur as a dimer. We conducted SPR analysis which suggested that PfHop is capable of self-association in presence or absence of ATP/ADP. Overall, our findings suggest that PfHop is a stress-inducible protein that directly associates with PfHsp70-1 and PfHsp90. In addition, the protein is capable of self-association. The findings suggest that PfHop serves as a module that brings these two prominent chaperones (PfHsp70-1 and PfHsp90) into a functional complex. Since PfHsp70-1 and PfHsp90 are essential for parasite growth, findings from this study are important towards the development of possible antimalarial inhibitors targeting the cooperation of these two chaperones.
Department of Biochemistry, School of Mathematics and Natural Sciences, University of Venda, Thohoyandou, 0950, South Africa.
Tawanda
T
Zininga
Department of Biochemistry, School of Mathematics and Natural Sciences, University of Venda, Thohoyandou, 0950, South Africa.
Stanely
S
Makumire
Department of Biochemistry & Microbiology, University of Zululand, P. Bag X1001, KwaDlangezwa, 3886, South Africa.
Grace Wairimu
GW
Gitau
Department of Biochemistry and Microbiology, Rhodes, Grahamstown, 6140, South Africa.
James M
JM
Njunge
Department of Biochemistry & Microbiology, University of Zululand, P. Bag X1001, KwaDlangezwa, 3886, South Africa.
Ofentse Jacob
OJ
Pooe
Parasitology, FB Biology, Philipps University Marburg, 35043, Marburg, Germany.
Hanna
H
Klimek
Parasitology, FB Biology, Philipps University Marburg, 35043, Marburg, Germany.
Robina
R
Scheurr
Flow cytometry core facility, Institute for Medical Microbiology, University Clinic Marburg, Marburg, Germany.
Hartmann
H
Raifer
Biotechnology Innovation Centre, Rhodes University, Grahamstown, 6140, South Africa.
Earl
E
Prinsloo
Parasitology, FB Biology, Philipps University Marburg, 35043, Marburg, Germany.
Jude M
JM
Przyborski
Department of Biochemistry and Microbiology, Rhodes, Grahamstown, 6140, South Africa.
Heinrich
H
Hoppe
Department of Biochemistry, School of Mathematics and Natural Sciences, University of Venda, Thohoyandou, 0950, South Africa.
Addmore
A
Shonhai
D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins