Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation.
Human skin-derived precursor cells are a multipotent stem cell population that resides within the dermis throughout adulthood. Human skin-derived precursor cells can be isolated, purified, and expanded in large quantities from any patient, in health and disease, and differentiated to mesodermal and ectodermal cell types. Recently, it was also found that they can be directed towards hepatic cells with acquired properties of toxicological relevance. As such, they represent a valuable cell source for the further development of human-relevant in vitro models for the identification and quantification of hepatotoxic compounds. In this chapter, a robust basic methodology to isolate, expand, and differentiate human skin-derived precursor cells into hepatic cells in a sequential and time-dependent way is provided.
Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium, jdekock@vub.ac.be.
Joery
J
De Kock
Robim M
RM
Rodrigues
Karolien
K
Buyl
Tamara
T
Vanhaecke
Vera
V
Rogiers
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D061251:Primary Cell Culture; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*