Analytical performance of the automated multianalyte point-of-care mariPOC® for the detection of respiratory viruses.
The analytical performance of mariPOC® respi test (ArcDia® Laboratories, Turku, Finland) was evaluated using nucleic acid amplification techniques (NAATs) as the gold standard. The mariPOC assay allows automated detection of antigens from 8 respiratory viruses: influenza A and B viruses, respiratory syncytial virus, adenovirus, human metapneumovirus, and parainfluenza viruses 1-3. Positive results from samples with high viral load are available in 20min. Nasopharyngeal aspirates (n=192) from patients with acute respiratory infection and from previously positive samples were analyzed by mariPOC and NAATs (Simplexa(TM) FluA/FluB & RSV kit [n=118] and Luminex® Respiratory virus panel xTAG® RVP FAST [n=74]). Sensitivity, specificity, positive predictive value, and negative predictive value of mariPOC were 85.4%, 99.2%, 95.9%, and 97%, respectively, and 84.6% of positive results were reported in 20min. The good analytical performance and extended portfolio of mariPOC show this rapid assay as a good alternative for the etiological diagnosis of acute respiratory infection in laboratories that are not equipped with molecular assays.
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas 2, 18012 Granada, Spain; Instituto de Investigación Biosanitaria de Granada, Calle Dr Azpitarte 4, 18012 Granada, Spain. Electronic address: saral.sanbonmatsu.sspa@juntadeandalucia.es.
Sara
S
Sanbonmatsu-Gámez
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas 2, 18012 Granada, Spain; Instituto de Investigación Biosanitaria de Granada, Calle Dr Azpitarte 4, 18012 Granada, Spain.
Mercedes
M
Pérez-Ruiz
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas 2, 18012 Granada, Spain.
Ana
A
Lara-Oya
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas 2, 18012 Granada, Spain; Instituto de Investigación Biosanitaria de Granada, Calle Dr Azpitarte 4, 18012 Granada, Spain.
Irene
I
Pedrosa-Corral
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas 2, 18012 Granada, Spain.
Cristina
C
Riazzo-Damas
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas 2, 18012 Granada, Spain; Instituto de Investigación Biosanitaria de Granada, Calle Dr Azpitarte 4, 18012 Granada, Spain.
José María
JM
Navarro-Marí
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000032:analysis*; D057205:Automation, Laboratory / Q000379:methods*; D002648:Child; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007231:Infant, Newborn; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D019095:Point-of-Care Systems; D011237:Predictive Value of Tests; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014777:Virus Diseases / Q000175:diagnosis*; D014780:Viruses / Q000145:classification / Q000302:isolation & purification*; D055815:Young Adult
D000956:Antigens, Viral
Respiratory virus; antigen detection; point-of-care; rapid test