Direct Detection of Escherichia coli Virulence Genes by Real-Time PCR in Fecal Samples from Bats in Brazil.
Guano samples from 412 Brazilian bats were screened with real-time PCR for the virulence genes (eae, est, elt, stx1, stx2, ehxA, invA, bfpA, aggR) representing five intestinal pathotypes of Escherichia coli. From 82 pooled samples, 22% contained Escherichia coli DNA, and eae, est, bfpA, aggR were detected.
1  VISAVET Health Surveillance Centre, Complutense University of Madrid, Avenida Puerta de Hierro s/n 28040 Madrid, Spain.|2  SaBio IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n 13071 Ciudad Real, Spain.
Adriana
A
Cabal
3  PPGBAN, Department of Zoology, Institute of Biosciences, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves 9500, Porto Alegre Rio Grande do Sul, 91540-000, Brazil.|4  PPGEC, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79090-900, Brazil.|5  Department of Biology and Center for Environmental and Marine Studies, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal.
Maria J
MJ
Pereira
6  Department of Zoology and Institute of Biological Sciences, University of Brasília, Campus Darcy Ribeiro, 70.910-900 Distrito Federal, Brazil.
Ludmilla M S
LM
Aguiar
1  VISAVET Health Surveillance Centre, Complutense University of Madrid, Avenida Puerta de Hierro s/n 28040 Madrid, Spain.
Lucas
L
Domínguez
5  Department of Biology and Center for Environmental and Marine Studies, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal.
Carlos
C
Fonseca
7  Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1352 Boyd Ave., St. Paul, Minnesota, USA.
Julio
J
Álvarez
8  Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25 Bonn 53127, Germany.
Jan F
JF
Drexler
2  SaBio IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n 13071 Ciudad Real, Spain.
Christian
C
Gortázar
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002685:Chiroptera; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D014774:Virulence