A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis.
Animal trypanosomosis is a disease that is distributed worldwide which results in huge economic losses due to reduced animal productivity. Endemic regions are often located in the countryside where laboratory diagnosis is costly or inaccessible. The establishment of simple, effective, and accurate field tests is therefore of great interest to the farming and veterinary sectors. Our study aimed to develop a simple, rapid, and sensitive immunochromatographic test (ICT) for animal trypanosomosis utilizing the recombinant tandem repeat antigen TeGM6-4r, which is conserved amongst salivarian trypanosome species. In the specificity analysis, TeGM6-4r/ICT detected all of Trypanosoma evansi-positive controls from experimentally infected water buffaloes. As expected, uninfected controls tested negative. All sera samples collected from Tanzanian and Ugandan cattle that were Trypanosoma congolense- and/or Trypanosoma vivax-positive by microscopic examination of the buffy coat were found to be positive by the newly developed TeGM6-4r/ICT, which was comparable to results from TeGM6-4r/ELISA (kappa coefficient [κ] = 0.78). TeGM6/ICT also showed substantial agreement with ELISA using Trypanosoma brucei brucei (κ = 0.64) and T. congolense (κ = 0.72) crude antigen, suggesting the high potential of TeGM6-4r/ICT as a field diagnostic test, both for research purposes and on-site diagnosis of animal trypanosomosis.
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
Thu-Thuy
TT
Nguyen
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
Ngasaman
N
Ruttayaporn
Laboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Yasuyuki
Y
Goto
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
Shin-ichiro
S
Kawazu
Laboratory Animal Facilities, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105-8461, Japan.
Tatsuya
T
Sakurai
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan. ircpmi@obihiro.ac.jp.
Noboru
N
Inoue
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D002020:Buffaloes; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D014346:Trypanosoma brucei brucei / Q000276:immunology / Q000302:isolation & purification; D014348:Trypanosoma congolense / Q000276:immunology; D016689:Trypanosoma vivax / Q000276:immunology / Q000302:isolation & purification; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis* / Q000469:parasitology
D000953:Antigens, Protozoan
Animal trypanosomosis; Immunochromatographic test; Tandem repeat antigen