Qualitative and Quantitative Analysis of Major Triterpenoids in Alismatis Rhizoma by High Performance Liquid Chromatography/Diode-Array Detector/Quadrupole-Time-of-Flight Mass Spectrometry and Ultra-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry.
Alismatis Rhizoma (AMR) is a well-known natural medicine with a long history in Chinese medicine and has been commonly used for treating a wide range of ailments related to dysuria, edema, nephropathy, hyperlipidaemia, diabetes, inflammation as well as tumors in clinical applications. Most beneficial effects of AMR are attributed to the presence of protostane terpenoids, the major active ingredients of Alismatis Rhizoma (AMR). In this study, a systematic high performance liquid chromatography/diode-array detector/quadrupole-time-of-flight mass spectrometry (HPLC-DAD-Q-TOF MS) and ultra-performance liquid chromatography/triple quadrupole mass spectrometry (UPLC-QqQ MS) method was developed for qualitative and quantitative analyses of the major AMR triterpenoids. First, a total of 25 triterpenoid components, including 24 known compounds and one new compound were identified by comparison with UV spectra, molecular ions and fragmentation behaviors of reference standards or the literature. Second, an efficient method was established for the rapid simultaneous determination of 14 representative triterpenoids by UPLC-QqQ MS. Forty-three batches of AMR were analyzed with linearity (r, 0.9980-0.9999), intra-day precision (RSD, 1.18%-3.79%), inter-day precision (RSD, 1.53%-3.96%), stability (RSD, 1.32%-3.97%), repeatability (RSD, 2.21%-4.25%), and recovery (98.11%-103.8%). These results indicated that new approaches combining HPLC-DAD-Q-TOF MS and UPLC-QqQ MS are applicable in the qualitative and quantitative analysis of AMR.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. zhaowanlitcm@126.com.|Institute of Nanostructured Functional materials, Huanghe Science and Technology College, Zhengzhou 450006, China. zhaowanlitcm@126.com.
Wanli
W
Zhao
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. 15880081619@163.com.
Xiaoqiang
X
Huang
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. lixy8556@163.com.
Xiaoyan
X
Li
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. rdfxzff@163.com.
Fangfang
F
Zhang
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. 18950490357@163.com.
Sainan
S
Chen
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. ymfjtcm@163.com.
Miao
M
Ye
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. huangmingqing3413@gmail.com.
Mingqing
M
Huang
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. yaoxuexuwen@163.com.
Wen
W
Xu
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. wsstcm@fjtcm.edu.cn.
Shuisheng
S
Wu
D034902:Alisma / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
D014315:Triterpenes
Alisma orientale (Sam.) Juzep.; Alismatis Rhizoma; QTOF mass spectrometry; QqQ mass spectrometry; quality control