MiR-661 contributed to cell proliferation of human ovarian cancer cells by repressing INPP5J expression.
Accumulating evidence has emerged important roles for microRNAs (miRNAs) participating in oncogenesis and growth of various cancers. We hypothesized that miR-661 played an important role in cell growth of ovarian cancer. Here, we found miR-661 was upregulated in human ovarian cancer cell lines and clinical tumor tissues. Our results revealed that miR-661 directly targeted INPP5J, thereby acting as tumor promoter in ovarian cancer cells by blocking cell proliferation. Importantly, we identified miR-661 as a positive regulator of INPP5J-induced AKT pathway. Taken together, our study sheds light onto the role of miR-661 as tumor promoter by targeting the INPP5J gene, and then promoting cell proliferation of ovarian cancer.
Department of Obstetrics and Gynecology, General Hospital of Jinan Military Command, Shandong 250031, People's Republic of China. Electronic address: tongyuzhudoctor@163.com.
Tongyu
T
Zhu
Department of Medical Information, General Hospital of Jinan Military Command. Shandong 250031, People's Republic of China.
Jing
J
Yuan
Department of Obstetrics and Gynecology, General Hospital of Jinan Military Command, Shandong 250031, People's Republic of China.
Yuzhi
Y
Wang
Department of Obstetrics and Gynecology, General Hospital of Jinan Military Command, Shandong 250031, People's Republic of China.
Cuiping
C
Gong
Department of Obstetrics and Gynecology, General Hospital of Jinan Military Command, Shandong 250031, People's Republic of China.
Yudou
Y
Xie
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China.
Hong
H
Li
D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
C543810:MIRN661 microRNA, human; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D010744:Phosphoric Monoester Hydrolases; C581758:INPP5J protein, human
Cell proliferation; Human ovarian cancer; INPP5J; miR-661