Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promoter.
The chromatin immunoprecipitation (ChIP) assay is a versatile technique used to evaluate the association of proteins with specific DNA regions both in vivo and in vitro. This assay can be used to identify proteins associated with a specific region of the genome, or the opposite, to identify the many regions of the genome associated with a particular protein. The ChIP assay can also be used to analyze binding of transcription factors, transcription cofactors, DNA replication factors, and DNA repair proteins. Here we describe a useful ChIP-qPCR protocol to examine the interaction of NFkB with the VEGF promoter in adult rat primary cardiomyocytes that have been mechanically stretched after attaching to the extracellular matrix protein laminin.
Department of Surgery, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, 96813, USA.
Chad B
CB
Walton
Michelle L
ML
Matter
D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D032383:Myocytes, Cardiac / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D042442:Vascular Endothelial Growth Factors / Q000235:genetics*
D016328:NF-kappa B; D042442:Vascular Endothelial Growth Factors