[Analysis of four flavonoids in Lysimachia clethroides using ionic liquid-assisted extraction].
In order to established a method for simultaneous determination of isoquercitrin, astragaline, quercetin and kaempferol in Lysimachia clethroides, the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM]PF6) methanol was used as the ultrasound-assisted extraction solvent combing with RP-HPLC. A Purospher star RP-C1 column was used with the mobile phase of aceto- nitrile, methanol and 0. 4% phosphate acid by gradient elution at the detection wavelength of 360 nm. The flow rate was 0.7 mL x min(-1), and the column temperature was the room temperature. Under the optimized conditions, the linear ranges were 2.54 x 10(-2)-2. 54, 2.50 x 10(-2)- 2.50, 1.54 x 10(-3)-0.154, 1.49 x 10(-3)-0.149 microg for isoquercitrin, astragaline, quercetin and kaempferol, respectively. The average recoveries of the four constituents were 101.1%, 98.90%, 101.0%, 101.6%, respectively. The method was green, simple, rapid and accurate, and provided a valid method for analysis of isoquercitrin, astragaline, quercetin and kaempferol in L. clethroides.
Jin-feng
JF
Wei
Zhi-juan
ZJ
Zhang
Dong-dong
DD
Li
Wei
W
Liu
Wen-yi
WY
Kang
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D052578:Ionic Liquids / Q000737:chemistry; D029623:Primulaceae / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D052578:Ionic Liquids