Effects of inferior splenic lobe pole fixation and gastrosplenic peritoneal membrane section on the vitality of the remanent of subtotal splenectomy in rats.
OBJECTIVE
To investigate the vitality of the spleen lower pole after subtotal splenectomy with suture to the stomach and after posterior peritoneal gastro-splenic membrane section, using macro and microscopic evaluations.


METHODS
Sixty Wistar rats were used in this study and were randomly distributed in the three groups: Group 1: (n=20), subtotal splenectomy with lower pole preservation, Group 2: (n=20) subtotal splenectomy with lower pole preservation and suture to the stomach, Group 3: subtotal splenectomy with lower pole preservation and posterior peritoneal gastrosplenic ligament section. The animals were sacrificed 45 days after the surgery and the spleen lower poles were removed for macroscopic and microscopic examination.


RESULTS
All animals in this series survived. No macroscopic differences were encountered between the groups. Microscopic evaluation observed statistic difference concerning fibrosis between group 1 and 3 (p ≤ 0.05), but the analysis for necrosis and inflammation presented no differences.


CONCLUSIONS
Vitality of the spleen lower pole after subtotal splenectomy is minimally modified when it is fixed to the stomach or when the posterior peritoneal gastrosplenic ligament is resected.
Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Fernanda Alves
FA
Mendonça
School of Medicine, EMESCAM, Brazil.
Felipe Poubel Timm do
FP
Carmo
School of Medicine, EMESCAM, Brazil.
Louise Goncalves
LG
Paris
School of Medicine, EMESCAM, Brazil.
Marya Duarte
MD
Pagotte
School of Medicine, EMESCAM, Brazil.
Fernanda Lube Antunes
FL
Pereira
Department of Pathology, UFMG, Belo Horizonte, MG, Brazil.
Paula Vieira Teixeira
PV
Vidigal
Department of Surgery, College of Health Sciences, EMESCAM, Vitoria, ES, Brazil.
Danilo Nagib Salomão
DN
Paulo
Department of Surgery, Medical School, UFMG, Belo Horizonte, MG, Brazil.
Tarcizo Afonso
TA
Nunes
D000818:Animals; D005240:Feasibility Studies; D005355:Fibrosis / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000473:pathology; D009929:Organ Size; D010537:Peritoneum / Q000473:pathology / Q000601:surgery*; D011184:Postoperative Period; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013154:Spleen / Q000473:pathology / Q000601:surgery*; D013156:Splenectomy / Q000379:methods*; D013270:Stomach / Q000601:surgery*; D016896:Treatment Outcome