Intermittent cold exposure improves glucose homeostasis associated with brown and white adipose tissues in mice.
OBJECTIVE
The discovery of different shades of fat has been implicated in the pathogenesis of obesity-related metabolic disorders. However, the effects of early and intermittent exposure to cold temperature on systemic metabolic changes in adult life remain unclear.


METHODS
To elucidate the impact of cold temperature exposure on metabolic function of adipose tissues, we investigated the glucose homeostasis, activation of brown adipose tissue (BAT) and "browning" of white adipose tissue (WAT) in mice in response to intermittent cold exposure. Mice were exposed to 4 °C, 2h per day and 5 days per week, for 14 weeks. Glucose homeostasis was tested via intraperitoneal glucose tolerance test and insulin tolerance test; body fat mass was evaluated using in vivo magnetic resonance imaging; BAT activity was detected primarily by positron emission tomography/computed tomography; and WAT "browning" was evaluated using immunohistochemistry.


RESULTS
Our results showed that a 14-week cold exposure improved glucose tolerance and enhanced insulin sensitivity, reduced the relative weights of epididymal and retroperitoneal WAT, increased expressions of UCP1 and PGC1α in subcutaneous adipose tissue.


CONCLUSIONS
Intermittent exposure to cold temperature in early life may improve systemic glucose homeostasis and induce WAT "browning", suggesting that an ambient cold temperature exposure may serve as a promising intervention to metabolic disorders.
Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, USA.
Tse-Yao
TY
Wang
Basic Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Cuiqing
C
Liu
Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA.
Aixia
A
Wang
Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, USA; Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA; Shanghai Key Laboratory of Meteorology and Health, Shanghai, China. Electronic address: sun.224@osu.edu.
Qinghua
Q
Sun
D002001:Adipose Tissue, Brown / Q000502:physiology*; D052436:Adipose Tissue, White / Q000502:physiology*; D000818:Animals; D003080:Cold Temperature; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D005947:Glucose
Adipose tissue; Cold exposure; Glucose homeostasis