Interleukin-17A Gene Expression in Morbidly Obese Women.
Data from recent studies conducted in rodent models and humans suggest that interleukin-17A (IL-17A) plays a role in the induction of inflammation in adipose tissue during obesity. The aim of this study was to assess the gene expression of IL-17A in adipose tissue of morbidly obese patients. We used RT-PCR to evaluate the expression of IL-17A and several adipo/cytokines in the visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) of 10 normal-weight control women (BMI < 25 kg/m2) and 30 morbidly obese women (MO, BMI > 40 kg/m2). We measured serum levels of IL-17A and adipo/cytokines in MO and normal weight women. IL-17A expression was significantly higher in VAT than in SAT in MO patients (p = 0.0127). It was very low in normal-weight controls in both VAT and SAT tissues. We found positive correlations between IL-17A and IL-6, lipocalin-2 and resistin in VAT of MO patients. The circulating level of IL-17A was higher in the normal-weight group than the MO patients (p = 0.032), and it was significantly related to adiponectin and TNFRII levels. In conclusion, IL-17A expression in VAT is increased in morbidly obese women, which suggests a link between obesity and innate immunity in low-grade chronic inflammation in morbidly obese women.
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. fernandoa.zapata@gmail.com.
Fernando
F
Zapata-Gonzalez
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. tauguet.hj23.ics@gencat.cat.|Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. tauguet.hj23.ics@gencat.cat.
Teresa
T
Auguet
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. gemma.aragones@iispv.cat.
Gemma
G
Aragonès
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. esther.guiu@urv.cat.
Esther
E
Guiu-Jurado
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. alba.berlanga@urv.cat.
Alba
A
Berlanga
Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. mgonzalez.hj23.ics@gencat.cat.
Salomé
S
Martinez
Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. andreumano@gmail.com.
Andreu
A
Martí
Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, IISPV, Universitat Rovira i Virgili (URV), Avinguda Doctor Josep Laporte, 2, 43204 Tarragona, Spain. fatima.sabench@urv.cat.
Fátima
F
Sabench
Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, IISPV, Universitat Rovira i Virgili (URV), Avinguda Doctor Josep Laporte, 2, 43204 Tarragona, Spain. mhernandezg@grupsagessa.com.
Mercé
M
Hernandez
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. caguilar.hj23.ics@gencat.cat.
Carmen
C
Aguilar
Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. jsirvent.hj23.ics@gencat.cat.
Joan Josep
JJ
Sirvent
Servei de Cirurgia, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. rjorba.hj23@gencat.cat.
Rosa
R
Jorba
Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, IISPV, Universitat Rovira i Virgili (URV), Avinguda Doctor Josep Laporte, 2, 43204 Tarragona, Spain. ddelcastillo@grupsagessa.com.
Daniel
D
Del Castillo
Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. crichart.hj23.ics@gencat.cat.|Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. crichart.hj23.ics@gencat.cat.
Cristóbal
C
Richart
D000328:Adult; D015992:Body Mass Index; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007249:Inflammation / Q000235:genetics* / Q000473:pathology; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis; D050152:Intra-Abdominal Fat / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics* / Q000473:pathology; D052243:Resistin / Q000096:biosynthesis
C508717:IL17A protein, human; D020381:Interleukin-17; D015850:Interleukin-6; C499624:RETN protein, human; D052243:Resistin
IL-17A; adipo/cytokine; morbid obesity; visceral adipose tissue