Detection of Common, Emerging and Uncommon VP4, and VP7 Human Group A Rotavirus Genotypes from Urban Sewage Samples in Uruguay.
Environmental approach has proven to be a useful tool for epidemiological studies demonstrating through environmental studies the diversity of viruses circulating in a given population. The aim of this study was to perform a phylogenetic characterization of the group A rotavirus (RVA) glycoprotein (G)- and protease-sensitive (P)-genotypes obtained from sewage samples (n = 116) collected in six cities of Uruguay during March 2011 to April 2013. A worldwide standardized semi-nested multiplex RT-PCR (SNM RT-PCR) protocol directed against VP4 and VP7 genes were conducted for RVA detection and consensual DNA fragments were submitted to nucleotide sequencing. P and/or G genotype was successfully determined by phylogenetic analysis in 61% (n = 37) of the positive samples obtained by SNM RT-PCR (n = 61). The RVA genotypes were as follow: G1 (n = 2), G2 (n = 14), G3 (n = 5), G12 (n = 2), P[4] (n = 4), P[8] (n = 16), and P[3] (n = 2). Interestingly, through phylogenetic analysis, emerging, and uncommon human genotypes could be detected. Results obtained from the comparison of RVA genotypes detected in the current study and Uruguayan RVA strains previously described for contemporary clinical pediatric cases showed that monitoring sewage may be a good screening option for a rapid and economical overview of the circulating genotypes in the surrounding human population and a useful approximation to study RVA epidemiology in a future vaccine monitoring program. The present study represents the first report in Uruguay that describes the phylogenetic diversity of RVA from urban sewage samples.
Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Regional Norte - CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
Luis Fernando Lopez
LF
Tort
Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Regional Norte - CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
Matías
M
Victoria
Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Regional Norte - CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
Andrés
A
Lizasoain
Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Regional Norte - CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
Mariana
M
García
Sección Virología, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay.
Mabel
M
Berois
Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay.
Juan
J
Cristina
Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, 21040-360, Brazil.
José Paulo Gagliardi
JP
Leite
Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, 21040-360, Brazil.
Mariela Martínez
MM
Gómez
Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, 21040-360, Brazil.
Marize Pereira
MP
Miagostovich
Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Regional Norte - CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay. rodneycolina1@gmail.com.
Rodney
R
Colina
D030541:Databases, Genetic; D004784:Environmental Monitoring; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012621:Seasons; D012722:Sewage / Q000821:virology*; D062211:Spatio-Temporal Analysis; D014507:Urbanization; D014583:Uruguay
D012367:RNA, Viral; D012722:Sewage
Epidemiology; G- and P-types; Group A rotavirus; Phylogeny; Sewage; Uruguay