Effects of metoclopramide-induced hyperprolactinemia on the prolactin and prolactin receptor expression of murine adrenal.
The aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin (PRL) and prolactin receptor's (PRLR) expression in the adrenal. For this purpose, a total of 12 animals with intact ovaries were allocated to two groups: G1 (saline solution) and G2 (metoclopramide). A total of 30 oophorectomized animals was randomized to five subgroups: G3 (saline solution), G4 (metoclopramide), G5 (metoclopramide + 17β-estradiol), G6 (metoclopramide + progesterone), and G7 (metoclopramide + 17β-estradiol + progesterone). Immunohistochemical analyses were evaluated semi-quantitatively. For PRLR, the area fraction of labeled cells (ALC) varied from 1 (0-10%) to 3 (> 50%). Based on the mean of the immunostaining intensity, G2 and G4 showed strong expression; G6 and G7 presented a mild reaction; and G1, G3, and G5 exhibited a weak reaction. Concerning PRL, the ALC varied from 1 (0-10%) to 3 (> 50%), and groups G6 and G7 showed a strong reaction; G2, G4, and G5 showed a mild reaction; and G1 and G3 exhibited a weak reaction. These findings suggest that metoclopramide-induced hyperprolactinemia increases PRL expression in the adrenal glands of mice. Furthermore, progesterone alone or in association with estrogen also increases PRL expression, but to a lesser extent.
a Laboratorio de Ginecologia Estrutural e Molecular (LIM-58) , Faculdade de Medicina da Universidade de Sao Paulo (FMUSP) , São Paulo , Brazil .|b Instituto de Ciencias da Saude da Universidade Paulista (ICS-UNIP) , São Paulo , Brazil , and.|c Department of Reproductive Medicine and Child Development , Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), University of Pisa , Pisa , Italy.
Vinícius Cestari
VC
do Amaral
a Laboratorio de Ginecologia Estrutural e Molecular (LIM-58) , Faculdade de Medicina da Universidade de Sao Paulo (FMUSP) , São Paulo , Brazil .
Priscilla Ludovico
PL
da Silva
a Laboratorio de Ginecologia Estrutural e Molecular (LIM-58) , Faculdade de Medicina da Universidade de Sao Paulo (FMUSP) , São Paulo , Brazil .
Kátia Candido
KC
Carvalho
c Department of Reproductive Medicine and Child Development , Molecular and Cellular Gynecological Endocrinology Laboratory (MCGEL), University of Pisa , Pisa , Italy.
Tommaso
T
Simoncini
a Laboratorio de Ginecologia Estrutural e Molecular (LIM-58) , Faculdade de Medicina da Universidade de Sao Paulo (FMUSP) , São Paulo , Brazil .
Gustavo Arantes Rosa
GA
Maciel
a Laboratorio de Ginecologia Estrutural e Molecular (LIM-58) , Faculdade de Medicina da Universidade de Sao Paulo (FMUSP) , São Paulo , Brazil .
José Maria
JM
Soares
a Laboratorio de Ginecologia Estrutural e Molecular (LIM-58) , Faculdade de Medicina da Universidade de Sao Paulo (FMUSP) , São Paulo , Brazil .
Edmund Chada
EC
Baracat
D000311:Adrenal Glands / Q000737:chemistry* / Q000187:drug effects; D000818:Animals; D004195:Disease Models, Animal; D004958:Estradiol / Q000008:administration & dosage; D004967:Estrogens / Q000097:blood; D005260:Female; D006966:Hyperprolactinemia / Q000139:chemically induced* / Q000378:metabolism; D007150:Immunohistochemistry; D008787:Metoclopramide / Q000008:administration & dosage*; D051379:Mice; D010052:Ovariectomy; D011374:Progesterone / Q000008:administration & dosage / Q000097:blood; D011388:Prolactin / Q000032:analysis* / Q000097:blood; D011981:Receptors, Prolactin / Q000032:analysis*
D004967:Estrogens; D011981:Receptors, Prolactin; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D008787:Metoclopramide
Adrenal gland; estrogens; hormone replacement therapy