Effect of mild hypothermia on the increase of CD11b+ Gr-1+ myeloid-derived suppressor cells induced by lipopolysaccharide in a mouse model of sepsis.
OBJECTIVE
This study aimed to investigate the influence of mild hypothermia on the number of CD11b+ Gr-1+ myeloid-derived suppressor cells (MDSCs) induced by lipopolysaccharide (LPS) injection in mice with sepsis.


METHODS
BALB/c mice were administered LPS to establish a mouse model of sepsis. Then, these mice were randomly divided into 3 groups: the mild hypothermia plus LPS group, the normothermia plus LPS group, and the LPS group. The normal control group was injected the same amount of 0.9% sodium chloride solution. The ratio of CD11b+ Gr-1+ MDSCs in the mouse spleen and bone marrow was determined at 6, 12, 24, 48, and 72 hours after LPS injection and after injected 0.9% sodium chloride solution.


RESULTS
Compared with the control group, the number of MDSCs in the spleen in the sepsis group increased gradually, and the difference was significant at 12 hours after injection (P<.01). Moreover, the number of MDSCs was the lowest in the mild hypothermia group, and there was a significant difference than the other 2 groups at 48 hours (P<.01). The number of MDSCs in the bone marrow increased gradually, and the difference between the sepsis and control groups was significant at 24 hours (P<.01). The number of MDSCs in the mild hypothermia group was the lowest, and there was a statistically significant difference than the other 2 groups (P<.05).


CONCLUSIONS
Mild hypothermia inhibited the production and accumulation of MDSCs induced by LPS administration in septic mice.
Department of Emergency, Clinical College of Pediatrics, Anhui Medical University, Anhui Provincial Children's Hospital, Hefei, China.
Xiaoshuang
X
Li
Department of Emergency, Clinical College of Pediatrics, Anhui Medical University, Anhui Provincial Children's Hospital, Hefei, China.
Li
L
Liu
Department of Emergency, Clinical College of Pediatrics, Anhui Medical University, Anhui Provincial Children's Hospital, Hefei, China.
Feifei
F
Luo
Comprehensive Laboratory, Basic Medical School of Anhui Medical University, Hefei, China.
Li
L
Gui
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, China.
Dazhi
D
Fan
Department of Emergency, Clinical College of Pediatrics, Anhui Medical University, Anhui Provincial Children's Hospital, Hefei, China. Electronic address: qilianxie@126.com.
Qilian
Q
Xie
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D039481:CD11b Antigen / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects*; D002681:China; D004195:Disease Models, Animal; D004926:Escherichia coli; D005434:Flow Cytometry; D007035:Hypothermia / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D022423:Myeloid Cells / Q000187:drug effects*; D019707:Receptors, Chemokine / Q000032:analysis; D018805:Sepsis / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D015996:Survival Rate
D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D008070:Lipopolysaccharides; D019707:Receptors, Chemokine