Combination of Praziquantel and Aspirin Minimizes Liver Pathology of Hamster Opisthorchis viverrini Infection Associated Cholangiocarcinoma.
Opisthorchiasis is one of the major risk factors for cholangiocarcinoma (CCA) in northeastern Thailand. An effective drug for killing this parasite is praziquantel. Recently, several reports have shown that with frequent use, praziquantel may itself be a CCA risk and can cause liver cell damage from an immunopathological response after parasite death. Aspirin has many properties including anti-inflammation and anti-cancer. Therefore, we use of aspirin (As) and praziquantel (Pz) to improve hepatobiliary system function in hamsters infected with Opisthorchis viverrini (OV) and or administered N-nitrosodimethylamine (ND). Livers of OVNDAsPz, appeared healthy macroscopically, suggesting slow progression of cholangiocarcinoma evident by extent of fibrosis and bile duct cell proliferation was less than OVND although aggregations of inflammatory cells remained. Proliferating cell nuclear antigen (PCNA), cytokeratin 19 (CK19), and cancer antigen (CA19-9) staining were strongly positive in OVND, but were only slight in OVNDAs. Moreover, OVNDAsPz, appeared a few inflammatory infiltrations, bile duct proliferation, fibrosis and CCA area than the OVNDAs group. Thirty seven point five percent of hamster in this group could not develop CCA. These findings suggest that using aspirin combination with praziquantel treatment can improve the hepatobiliary system after O. viverrini infection and reduce the risk of CCA.
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.|Faculty of Agricultural Technology, Burapha University Sakaeo Campus, Sakaeo, 27160, Thailand.
Pakkayanee
P
Sudsarn
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. bthida@kku.ac.th.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand. bthida@kku.ac.th.
Thidarut
T
Boonmars
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Wipaporn
W
Ruangjirachuporn
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand.|Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Nisana
N
Namwat
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand.|Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Watcharin
W
Loilome
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand.|Rajamangala University of Technology Isan Sakonnakhon Campus, Sakonnakhon, 47160, Thailand.
Pranee
P
Sriraj
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand.|Rajamangala University of Technology Isan Sakonnakhon Campus, Sakonnakhon, 47160, Thailand.
Ratchadawan
R
Aukkanimart
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand.
Wonkchalee
W
Nadchanan
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand.
Songsri
S
Jiraporn
D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D001241:Aspirin / Q000008:administration & dosage*; D001650:Bile Duct Neoplasms / Q000469:parasitology / Q000473:pathology / Q000517:prevention & control*; D001653:Bile Ducts, Intrahepatic / Q000187:drug effects* / Q000469:parasitology / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D018281:Cholangiocarcinoma / Q000469:parasitology / Q000473:pathology / Q000517:prevention & control*; D006224:Cricetinae; D004359:Drug Therapy, Combination; D005260:Female; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008647:Mesocricetus; D009889:Opisthorchiasis / Q000188:drug therapy* / Q000469:parasitology / Q000473:pathology; D009891:Opisthorchis / Q000472:pathogenicity; D011223:Praziquantel / Q000008:administration & dosage*
D000871:Anthelmintics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D014408:Biomarkers, Tumor; D011223:Praziquantel; D001241:Aspirin
Aspirin; Cholangiocarcinoma; N-nitrosodimethylamine; Opisthorchis viverrini; Praziquantel; Syrian hamster