An untargeted global metabolomic analysis reveals the biochemical changes underlying basal resistance and priming in Solanum lycopersicum, and identifies 1-methyltryptophan as a metabolite involved in plant responses to Botrytis cinerea and Pseudomonas syringae.
In this study, we have used untargeted global metabolomic analysis to determine and compare the chemical nature of the metabolites altered during the infection of tomato plants (cv. Ailsa Craig) with Botrytis cinerea (Bot) or Pseudomonas syringae pv. tomato DC3000 (Pst), pathogens that have different invasion mechanisms and lifestyles. We also obtained the metabolome of tomato plants primed using the natural resistance inducer hexanoic acid and then infected with these pathogens. By contrasting the metabolomic profiles of infected, primed, and primed + infected plants, we determined not only the processes or components related directly to plant defense responses, but also inferred the metabolic mechanisms by which pathogen resistance is primed. The data show that basal resistance and hexanoic acid-induced resistance to Bot and Pst are associated with a marked metabolic reprogramming. This includes significant changes in amino acids, sugars and free fatty acids, and in primary and secondary metabolism. Comparison of the metabolic profiles of the infections indicated clear differences, reflecting the fact that the plant's chemical responses are highly adapted to specific attackers. The data also indicate involvement of signaling molecules, including pipecolic and azelaic acids, in response to Pst and, interestingly, to Bot. The compound 1-methyltryptophan was shown to be associated with the tomato-Pst and tomato-Bot interactions as well as with hexanoic acid-induced resistance. Root application of this Trp-derived metabolite also demonstrated its ability to protect tomato plants against both pathogens.
Grup de Bioquímica i Biotecnología, Àrea de Fisiologa Vegetal, Departament de Ciències Agràries y del Medi Natural, Escola Superior de Tecnología i Ciències Experimentals, Universitat Jaume I, Castelló, Spain.
Gemma
G
Camañes
Grup de Bioquímica i Biotecnología, Àrea de Fisiologa Vegetal, Departament de Ciències Agràries y del Medi Natural, Escola Superior de Tecnología i Ciències Experimentals, Universitat Jaume I, Castelló, Spain.
Loredana
L
Scalschi
Grup de Bioquímica i Biotecnología, Àrea de Fisiologa Vegetal, Departament de Ciències Agràries y del Medi Natural, Escola Superior de Tecnología i Ciències Experimentals, Universitat Jaume I, Castelló, Spain.
Begonya
B
Vicedo
Departamento de Bioquímica y Biología Molecular, Universitat de València, Instituto de Agroquímica y Tecnología de los Alimentos-Consejo Superior de Investigaciones Científicas, Valencia, Spain.
Carmen
C
González-Bosch
Grup de Bioquímica i Biotecnología, Àrea de Fisiologa Vegetal, Departament de Ciències Agràries y del Medi Natural, Escola Superior de Tecnología i Ciències Experimentals, Universitat Jaume I, Castelló, Spain.
Pilar
P
García-Agustín
D020171:Botrytis / Q000502:physiology*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000378:metabolism* / Q000382:microbiology*; D055432:Metabolomics; D044224:Pseudomonas syringae / Q000502:physiology*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000378:metabolism
D014364:Tryptophan; C525396:1-methyltryptophan
Botrytis cinerea; Pseudomonas syringae; hexanoic acid; induced resistance; priming; tomato plants