Non-Lethal Heat Shock of the Asian Green Mussel, Perna viridis, Promotes Hsp70 Synthesis, Induces Thermotolerance and Protects Against Vibrio Infection.
Mild heat stress promotes thermotolerance and protection against several different stresses in aquatic animals, consequences correlated with the accumulation of heat shock protein 70 (Hsp70). The purpose of this study was to determine if non-lethal heat shock (NLHS) of the Asian green mussel, Perna viridis, an aquatic species of commercial value, promoted the production of Hsp70 and enhanced its resistance to stresses. Initially, the LT50 and LHT for P. viridis were determined to be 42°C and 44°C, respectively, with no heat shock induced death of mussels at 40°C or less. Immunoprobing of western blots revealed augmentation of constitutive (PvHsp70-1) and inducible (PvHsp70-2) Hsp70 in tissue from adductor muscle, foot, gill and mantel of P. viridis exposed to 38°C for 30 min followed by 6 h recovery, NLHS conditions for this organism. Characterization by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that PvHsp70-1 and PvHsp70-2 respectively corresponded most closely to Hsp70 from P. viridis and Mytilus galloprovincialis. Priming of adult mussels with NLHS promoted thermotolerance and increased resistance to V. alginolyticus. The induction of Hsp70 in parallel with enhanced thermotolerance and improved protection against V. alginolyticus, suggests Hsp70 functions in P. viridis as a molecular chaperone and as a stimulator of the immune system.
Institute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), 21030, Kuala Terengganu, Malaysia.
Nor Afiqah
NA
Aleng
Institute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), 21030, Kuala Terengganu, Malaysia; School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu (UMT), 21030, Kuala Terengganu, Malaysia.
Yeong Yik
YY
Sung
Department of Biology, Dalhousie University, Halifax, NS, B3H 4R2, Canada.
Thomas H
TH
MacRae
Institute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), 21030, Kuala Terengganu, Malaysia; School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu (UMT), 21030, Kuala Terengganu, Malaysia.
Mohd Effendy
ME
Abd Wahid
D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D007113:Immunity, Innate / Q000276:immunology; D013058:Mass Spectrometry; D049883:Perna; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology* / Q000517:prevention & control*
D018840:HSP70 Heat-Shock Proteins