Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis.
Maize chlorotic mottle virus (MCMV) is the only member of the Machlomovirus genus in the family Tombusviridae. Here, we obtained the Cryo-EM structure of MCMV by single particle analysis with most local resolution at approximately 4 Å. The Cα backbone was built based on residues with bulky side chains. The resolved C-terminus of the capsid protein subunit and obvious openings at the 2-fold axis demonstrated the compactness of the asymmetric unit, which indicates an important role in the stability of MCMV. The Asp116 residue from each subunit around the 5-fold and 3-fold axes contributed to the negative charges in the centers of the pentamers and hexamers, which might serve as a solid barrier against the leakage of genomic RNA. Finally, the loops most exposed on the surface were analyzed and are proposed to be potential functional sites related to MCMV transmission.
Institute of Insect Science, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Chun-Yan
CY
Wang
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Qin-Fen
QF
Zhang
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Yuan-Zhu
YZ
Gao
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Xue-Ping
XP
Zhou
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Gang
G
Ji
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Xiao-Jun
XJ
Huang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: jhong@zju.edu.cn.
Jian
J
Hong
Institute of Insect Science, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. Electronic address: chxzhang@zju.edu.cn.
Chuan-Xi
CX
Zhang
D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure; D036022:Capsid Proteins / Q000737:chemistry* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000737:chemistry / Q000821:virology; D011487:Protein Conformation; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry*; D016415:Sequence Alignment; D055672:Static Electricity; D019183:Tombusviridae / Q000737:chemistry* / Q000648:ultrastructure; D003313:Zea mays / Q000737:chemistry / Q000821:virology*
D036022:Capsid Proteins; D021122:Protein Subunits
MCMV; Single particle; Three-dimensional structure; Transmission