Simultaneous encapsulation of magnetic nanoparticles and zinc phthalocyanine in poly(methyl methacrylate) nanoparticles by miniemulsion polymerization and in vitro studies.
The aim of this work was the simultaneous encapsulation of magnetic nanoparticles (MNPs) and zinc(II) phthalocyanine (ZnPc) in poly(methyl methacrylate) (PMMA) (MNPsZnPc-PMMA) nanoparticles (NPs) by miniemulsion polymerization and to evaluate the photobiological activity and/or hyperthermia (HPT) against human glioblastoma cells (U87MG). MNPsZnPc-PMMA NPs presented an average diameter of 104 ± 2.5 nm with a polydispersity index (PdI) of 0.14 ± 0.03 and negative surface charge - 47 ± 2.2 mV (pH 7.4 ± 0.1). The encapsulation efficiency (EE%) of ZnPc was 85.7% ± 1.30. The release of ZnPc from PMMA NPs was slow and sustained without the presence of burst effect, indicating a homogeneous distribution of the drug in the polymeric matrix. In the biological assay, MNPsZnPc-PMMA NPs showed considerable cytotoxic effect on U87MG cells only after activation with visible light at 675 nm (photodynamic therapy, PDT) or after application of an alternating magnetic field. The simultaneous encapsulation of MNPs and ZnPc in a drug delivery system with sustained release can be a new alternative for cancer treatment leading to significant tumor regression after minimum doses of heat dissipation and light.
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
Paulo Emilio
PE
Feuser
Center of Nanotechnology and Tissue Engineers, Photobiology and Photomedicine Research, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Andrielle Castilho
AC
Fernandes
School of Chemistry, Federal University of Rio Janeiro, Brazil.
Marcio
M
Nele
Department of Physics, Federal University of Santa Catarina, Brazil.
Alexandre da Cas
ADC
Viegas
Faculty of Pharmacy, Federal University of Rio Janeiro, Brazil.
Eduardo
E
Ricci-Junior
Center of Nanotechnology and Tissue Engineers, Photobiology and Photomedicine Research, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Antônio Claudio
AC
Tedesco
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
Claudia
C
Sayer
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil. Electronic address: pedro.h.araujo@ufsc.br.
Pedro Henrique Hermes
PHH
de Araújo
D000970:Antineoplastic Agents / Q000008:administration & dosage; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004655:Emulsions; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D008027:Light; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D053758:Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D009942:Organometallic Compounds / Q000737:chemistry*; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D017967:Zinc Compounds
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004655:Emulsions; D007211:Indoles; D054833:Isoindoles; D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D019904:Polymethyl Methacrylate
Hyperthermia; Miniemulsion polymerization; Photodynamic therapy; Polymeric nanoparticles