Revealing the trehalose mediated inhibition of protein aggregation through lysozyme-silver nanoparticle interaction.
We propose a facile and robust carbohydrate-mediated method for the prevention of nanoparticle induced denaturation and aggregation of proteins. Using label-free plasmon-enhanced Raman spectroscopy measurements, the mechanistic principles of trehalose stabilization in a model protein-nanoparticle system are elucidated for the first time, facilitating its further application in diagnostic and therapeutic nanoplex development.
Light Scattering Laboratory, Chemistry & Physics of Materials Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore 560 064, India. cbhas@jncasr.ac.in.
Soumik
S
Siddhanta
Ishan
I
Barman
Chandrabhas
C
Narayana
D053768:Metal Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D066329:Protein Aggregates; D011485:Protein Binding; D011489:Protein Denaturation; D012834:Silver / Q000737:chemistry; D014199:Trehalose / Q000737:chemistry*
D066329:Protein Aggregates; D012834:Silver; D014199:Trehalose; D009113:Muramidase