Broccoli seed extract: Genotoxicity and subchronic toxicity studies.
Potential health benefits have been attributed to broccoli consumption. Hence, there is potential for use of broccoli seed extract (BSE) in food or for use as a dietary supplement. To assess the potential safety of a BSE product, three genotoxicity experiments, including an Ames, in vivo mouse micronucleus, and in vivo mouse sperm abnormality assay, were carried out. BSE was subject to an acute oral toxicity test and was evaluated in a 30-day feeding study in rats. BSE showed no mutagenic activity in the Ames assay and no evidence of genotoxic potential in the in vivo assays at doses up to 10 g/kg body weight (bw). The LD50 of BSE in rats was >10 g/kg bw/d. In the 30-day feeding study, in which BSE was administered in the diet to provide doses of 0, 0.3, 1.0, or 3.0 g/kg bw/d, no toxicological significant effects were noted on body weight, body weight gain, organ weights, or on the results of hematological, clinical chemistry and histopathological evaluations. The no-observed-adverse-effect level was considered to be 3.0 g/kg bw/d, the highest dose tested. Collectively, these results support the safe use of BSE as a food ingredient or product.
Key Laboratory of Food Safety Risk Assessment of National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
Yu
Y
Zhou
Key Laboratory of Food Safety Risk Assessment of National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
Hui
H
Yang
Key Laboratory of Food Safety Risk Assessment of National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
Yongning
Y
Li
Intertek Scientific & Regulatory Consultancy, 2233 Argentia Rd., Suite 201, Mississauga, ON L5N 2X7, Canada.
B
B
Lynch
Key Laboratory of Food Safety Risk Assessment of National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China. Electronic address: jiaxudong@cfsa.net.cn.
Xudong
X
Jia
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001937:Brassica / Q000009:adverse effects*; D019587:Dietary Supplements / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods; D009153:Mutagens / Q000009:adverse effects; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000009:adverse effects*; D023382:Toxicity Tests, Acute / Q000379:methods
D009153:Mutagens; D010936:Plant Extracts
Broccoli seed extract; Genotoxicity; Mouse sperm abnormality assay; Rat; Subchronic toxicity