Retinoic acid aliphatic amide inhibits the AMPK-HIF-1α pathway in human ovarian cancer.
Ovarian carcinoma the commonly observed gynecological cancers has a high mortality rate. In the present study effect of retinoic acid aliphatic amide (RACA) in ovarian cancer cells was investigated using proliferation, migration and invasion assays. Western blot was used to examine the Bcl-2, cleaved caspase 3, p-ERK, MMP-2, p-FAK, P-P38, p-AMPKα and HIF-1α protein expression. CoCl2 was used to induce HIF-1α expression in SKOV3ip. 1 and HEY-A8 cells. The results revealed that RACA treatment prompted cell proliferation, invasion and migration but inhibited apoptosis of SKOV3ip. 1 and HEY-A8 cells. RACA treatment also induced upregulation of Bcl-2 and MMP-2, activation of p-P38, p-ERK and p-FAK, inhibition of cleaved caspase 3. RACA treatment also caused upregulatation of HIF-1α in ovarian cells with the activation of p-AMPKα. Upregulation of HIF-1α expression in CoCl2-treated cancer cells resulted in decrease in SDHB. Thus RACA plays a key role in cell proliferation, invasion, migration and apoptosis of human ovarian carcinoma through AMPK-HIF-1α pathway.
Department of Obstetrics and Gynecology, The Affiliated TCM Hospital of Sichuan Medical University Sichuan, 646000, China.
Xiao-Lan
XL
Yu
Researh Center for Drug and Functional Food of Sichuan Medical University Sichuan, 646000, China.
Ji-Yi
JY
Xia
Department of Obstetrics and Gynecology, Affiliated Hospital of Sichuan Medical University Sichuan, 646000, China.
Hai-Qiong
HQ
Ye
Department of Obstetrics and Gynecology, The Hosipital of Traditional Chinese Medicine of Leshan Sichuan, 614000, China.
Xia
X
Li
Department of Obstetrics and Gynecology, Affiliated Hospital of Sichuan Medical University Sichuan, 646000, China.
Yu-Jiao
YJ
Zhang
Department of Obstetrics and Gynecology, Affiliated Hospital of Sichuan Medical University Sichuan, 646000, China.
Xi-Guang
XG
Mao
D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000970:Antineoplastic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D014212:Tretinoin; D055372:AMP-Activated Protein Kinases
AMPK; HIF-1α; Tumor progression; apoptosis; gene silence; ovarian carcinoma