Changes of stress oxidative enzymes in rat mammary tissue, blood and milk after experimental mastitis induced by E. coli lipopolysaccharide.
The present study investigated the effects of E. coli lipopolysaccharide (LPS) induced mastitis model in rat on the activity of antioxidant enzyme systems. To achieve this purpose, E. coli LPS were infused into the mammary gland of 12 rats 72 hr postpartum and compared with 12 rats in control group infused intramammary placebo sterile pyrogene - free, physiological saline. The antioxidant activities of the enzymes, superoxide dismutase, glutathione peroxidase, and catalase together with ‎‏malondialdehyde (MDA) level were assayed in blood serum, milk and mammary tissue. Results obtained showed that, the antioxidant enzyme activities in milk, blood serum and mammary tissue were significantly decreased while the level of MDA, the indicator of lipid peroxidation were significantly increased following intramammary inoculation of LPS compared to the control animals. Histopathological examination also revealed the infiltration of inflammatory cells in mammary tissue and disruption of acinar structure and acinar lumina in mastitic rats. The results indicated that E. coli LPS-induced ‎mastitis could alter antioxidant enzymes and increase lipid peroxidation.
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Hadi
H
Eslami
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Rooz Ali
RA
Batavani
Department of Internal Medicine and ClinicalPathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Siamak
S
Asr I-Rezaei
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Rahim
R
Hobbenaghi
Antioxidant enzymes; Lipopolysaccharide; Malondialdehyde; Mastitis; Rat