In vivo analysis of trypanosome mitochondrial RNA function by artificial site-specific RNA endonuclease-mediated knockdown.
Trypanosomes possess a unique mitochondrial genome called the kinetoplast DNA (kDNA). Many kDNA genes encode pre-mRNAs that must undergo guide RNA-directed editing. In addition, alternative mRNA editing gives rise to diverse mRNAs and several kDNA genes encode open reading frames of unknown function. To better understand the mechanism of RNA editing and the function of mitochondrial RNAs in trypanosomes, we have developed a reverse genetic approach using artificial site-specific RNA endonucleases (ASREs) to directly silence kDNA-encoded genes. The RNA-binding domain of an ASRE can be programmed to recognize unique 8-nucleotide sequences, allowing the design of ASREs to cleave any target RNA. Utilizing an ASRE containing a mitochondrial localization signal, we targeted the extensively edited mitochondrial mRNA for the subunit A6 of the F0F1 ATP synthase (A6) in the procyclic stage of Trypanosoma brucei. This developmental stage, found in the midgut of the insect vector, relies on mitochondrial oxidative phosphorylation for ATP production with A6 forming the critical proton half channel across the inner mitochondrial membrane. Expression of an A6-targeted ASRE in procyclic trypanosomes resulted in a 50% reduction in A6 mRNA levels after 24 h, a time-dependent decrease in mitochondrial membrane potential (ΔΨm), and growth arrest. Expression of the A6-ASRE, lacking the mitochondrial localization signal, showed no significant growth defect. The development of the A6-ASRE allowed the first in vivo functional analysis of an edited mitochondrial mRNA in T. brucei and provides a critical new tool to study mitochondrial RNA biology in trypanosomes.
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA.
Anthony J
AJ
Szempruch
Department of Pharmacology, University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Rajarshi
R
Choudhury
Department of Pharmacology, University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Zefeng
Z
Wang
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA.
Stephen L
SL
Hajduk
D000818:Animals; D004720:Endonucleases / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D012313:RNA / Q000235:genetics*; D017393:RNA Editing; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan; D012313:RNA; D004720:Endonucleases
ATPase subunit 6; African trypanosome; RNA editing; artificial site-specific RNA endonuclease (ASRE); kinetoplast DNA; mitochondria