Isolation and characterisation of non-anaerobic butanol-producing symbiotic system TSH06.
Butanol-producing microorganisms are all obligate anaerobes. In this study, a unique symbiotic system TSH06 was isolated to be capable of producing butanol under non-anaerobic condition. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S ribosomal RNA (rRNA) revealed that two strains coexist in TSH06. The two strains were identical to Clostridium acetobutylicum and Bacillus cereus, respectively. They were isolated individually and named as C. acetobutylicum TSH1 and B. cereus TSH2. C. acetobutylicum TSH1 is a butanol-producing, obligate anaerobic strain. Facultative anaerobic B. cereus TSH2 did not possess the ability of butanol production; however, it offered C. acetobutylicum TSH1 the viability under non-anaerobic condition. Moreover, B. cereus TSH2 enhanced butanol yield and speed of fermentation. TSH06 produced 12.97 g/L butanol and 15.39 g/L total solvent under non-anaerobic condition, which is 25 and 24 %, respectively, higher than those of C. acetobutylicum TSH1. In addition, TSH06 produced butanol faster under non-anaerobic condition than under anaerobic condition. Butanol accounted for more than 80 % of total solvent, which is higher than the known report. TSH06 was stable during passage. In all, TSH06 is a promising candidate for industrialisation of biobutanol with high yield, high butanol proportion, easy-handling and time-saving system. These results demonstrated the potential advantage of symbiosis. This study also provides a promising strategy for butanol fermentation.
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Genyu
G
Wang
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Pengfei
P
Wu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.|Food College of Shihezi University, Shihezi, Xinjiang, 832000, China.
Ya
Y
Liu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Shuo
S
Mi
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Shuai
S
Mai
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Chunkai
C
Gu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Gehua
G
Wang
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China. liuhongjuan@mail.tsinghua.edu.cn.
Hongjuan
H
Liu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China. zhangja@tsinghua.edu.cn.
Jianan
J
Zhang
Statoil Petroleum AS, N-4035, Stavanger, Norway.
Børre Tore
BT
Børresen
Statoil Petroleum AS, N-4035, Stavanger, Norway.
Evy
E
Mellemsæther
Statoil Petroleum AS, N-4035, Stavanger, Norway.
Hans Kristian
HK
Kotlar
D000332:Aerobiosis; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D059013:Microbial Consortia; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000440:Butanols; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
Biofuel; Butanol; Non-anaerobic fermentation; Symbiosis; TSH06