Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice.
Polygonatum sibiricum polysaccharide (PSP) is a traditional Chinese medicine and is widely used to treat many diseases for hundreds of years conventionally. This study was to access the effects of PSP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in the mice. Cells collected from BALB/C mice in the bone marrow were isolated and cultured with osteogenic medium (OM) with different concentrations of PSP. The proliferation and morphological changes of BMSCs were observed using an inverted microscope. Flow cytometric analysis was used to identify the BMSCs. MTT test was performed to analyze the proliferation and viability of the cells. ELISA was used to determine the expression levels of alkaline phosphatase (ALP), osteocalcin (OC), N-terminal propeptide of type I procollagen (PINP) and bone morphogenetic protein-2 (BMP-2). Immunocytochemistry and western blot were respectively used to determine the expressions of bone sialoprotein (BSP) and SPARC/osteonectin (OSN). The growth curves of the proliferation and differentiation of the Control, OM, 17β-E2 and PSP groups were increased. Compared to the Control and OM groups, the expression levels of ALP, OC, PINP and BMP-2 were significantly increased in the PSP induced group (P<0.05). Immunocytochemistry and western blot showed that BSP and SPARC were increased after induction of PSP compared to the OM group (P<0.05). The study demonstrates that PSP promotes the proliferation and enhances the viability of BMSCs during osteogenic differentiation. Therefore, PSP may be a potential treatment of osteoporosis in the clinic.
Department of Osteopathia, The First Affiliated Hospital of Guangxi Medical University Nanning, Guangxi, China.
Shaohui
S
Zong
College of Public Hygiene of Guangxi Medical University Nanning, Guangxi, China.
Gaofeng
G
Zeng
Graduate School of Guangxi Medical University Nanning, Guangxi, China.
Bin
B
Zou
Graduate School of Guangxi Medical University Nanning, Guangxi, China.
Keke
K
Li
Graduate School of Guangxi Medical University Nanning, Guangxi, China.
Ye
Y
Fang
College of Public Hygiene of Guangxi Medical University Nanning, Guangxi, China.
Li
L
Lu
College of Public Hygiene of Guangxi Medical University Nanning, Guangxi, China.
Deqiang
D
Xiao
College of Public Hygiene of Guangxi Medical University Nanning, Guangxi, China.
Zhiyong
Z
Zhang
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D050071:Bone Density Conservation Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010641:Phenotype; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D031384:Polygonatum / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013997:Time Factors
D015415:Biomarkers; D050071:Bone Density Conservation Agents; D010936:Plant Extracts; D011134:Polysaccharides
Polygonatum sibiricum polysaccharide; alkaline phosphatase; bone marrow mesenchymal stem cells; osteoblasts; osteocalcin