ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors.
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
Christian
C
Schmidl
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
André F
AF
Rendeiro
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
Nathan C
NC
Sheffield
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.|Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.|Max Planck Institute for Informatics, Saarbrücken, Germany.
Christoph
C
Bock
D047369:Chromatin Immunoprecipitation / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D015894:Genome, Human; D006657:Histones / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism*
D006657:Histones; D014157:Transcription Factors