Design of a mouse restraint for synchrotron-based computed tomography imaging.
High-resolution computed tomography (CT) imaging of a live animal within a lead-lined synchrotron light hutch presents several unique challenges. In order to confirm that the animal is under a stable plane of anaesthesia, several physiological parameters (e.g. heart rate, arterial oxygen saturation, core body temperature and respiratory rate) must be remotely monitored from outside the imaging hutch. In addition, to properly scan the thoracic region using CT, the animal needs to be held in a vertical position perpendicular to the fixed angle of the X-ray beam and free to rotate 180°-360°. A new X-ray transparent mouse restraint designed and fabricated using computer-aided design software and three-dimensional rapid prototype printing has been successfully tested at the Biomedical Imaging and Therapy bending-magnet (BMIT-BM) beamline at the Canadian Light Source.
Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Kenneth B
KB
Gagnon
Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Sally
S
Caine
Biomedical Engineering, University of Saskatchewan, Saskatoon, SK, Canada.
Nazanin
N
Samadi
Physics and Engineering Physics, University of Saskatchewan, Saskatoon, SK, Canada.
Mercedes
M
Martinson
Research Services and Ethics Office, University of Saskatchewan, Saskatoon, SK, Canada.
Melanie
M
van der Loop
Research Services and Ethics Office, University of Saskatchewan, Saskatoon, SK, Canada.
Jane
J
Alcorn
Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
L Dean
LD
Chapman
Biomedical Imaging and Therapy Beamlines, Canadian Light Source, Saskatoon, SK, Canada.
George
G
Belev
Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Helen
H
Nichol
D000818:Animals; D017076:Computer-Aided Design; D003433:Crosses, Genetic; D004867:Equipment Design; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008904:Miniaturization; D066330:Printing, Three-Dimensional; D012149:Restraint, Physical / Q000295:instrumentation*; D013047:Specific Pathogen-Free Organisms; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed / Q000295:instrumentation*
X-ray imaging; anaesthesia; computer-aided design; remote physiological monitoring; three-dimensional printing