Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China.
Canine distemper virus (CDV) is a major pathogen not only in raccoon dogs but also in a variety of carnivorous animals, including domesticated animals, particularly if they have not been vaccinated. In this study, a wild-type strain of CDV was isolated from lung tissue from a raccoon dog kept at a fur farm in Jilin Province, China. Cytopathic effects typical of CDV infection were observed after three blind passages in Vero cells, yielding a virus titer of 10(4.6) TCID50/mL. Virus identification was carried out by RT-PCR, immunofluorescence, electron microscopy, and genome sequencing. The results showed that the isolated virus, termed the SY strain, corresponded to the Asia-1 genotype of CDV and has a genome of 15,690 nucleotides. This represents the first complete nucleotide sequence of a CDV strain circulating in raccoon dogs in China.
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Yuening
Y
Cheng
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China. tcswjk@126.com.
Jianke
J
Wang
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Miao
M
Zhang
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Jianjun
J
Zhao
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Xiqun
X
Shao
Hebei Normal University of Science and Technology, Changli, 066600, China.
Zengjun
Z
Ma
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Hang
H
Zhao
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Peng
P
Lin
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China.
Hua
H
Wu
D000818:Animals; D002681:China; D016000:Cluster Analysis; D003588:Cytopathogenic Effect, Viral; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D008168:Lung / Q000821:virology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D046031:Raccoon Dogs / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014776:Virus Cultivation
D012367:RNA, Viral
Canine distemper virus; Genome sequence; H gene; Raccoon dog