High-yield isolation of extracellular vesicles using aqueous two-phase system.
Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.
Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Hyunwoo
H
Shin
Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Chungmin
C
Han
Department of Biomedical Engineering, College of Engineering, Biointerfaces Institute, University of Michigan, 2800 Plymouth Rd, Ann Arbor, USA.
Joseph M
JM
Labuz
School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Jiyoon
J
Kim
Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Jongmin
J
Kim
Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Siwoo
S
Cho
Department of Life Sciences, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Yong Song
YS
Gho
1] Department of Biomedical Engineering, College of Engineering, Biointerfaces Institute, University of Michigan, 2800 Plymouth Rd, Ann Arbor, USA [2] Macromolecular Science and Engineering Center, College of Engineering, Biointerfaces Institute, University of Michigan, 2800 Plymouth Rd, Ann Arbor, USA.
Shuichi
S
Takayama
1] Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea [2] School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
Jaesung
J
Park
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D003911:Dextrans / Q000737:chemistry; D000067128:Extracellular Vesicles / Q000737:chemistry* / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D014867:Water / Q000737:chemistry*
D003911:Dextrans; D014867:Water; D011092:Polyethylene Glycols