Proteomics Insights into the Biomass Hydrolysis Potentials of a Hypercellulolytic Fungus Penicillium funiculosum.
The quest for cheaper and better enzymes needed for the efficient hydrolysis of lignocellulosic biomass has placed filamentous fungi in the limelight for bioprospecting research. In our search for efficient biomass degraders, we identified a strain of Penicillium funiculosum whose secretome demonstrates high saccharification capabilities. Our probe into the secretome of the fungus through qualitative and label-free quantitative mass spectrometry based proteomics studies revealed a high abundance of inducible CAZymes and several nonhydrolytic accessory proteins. The preferential association of these proteins and the attending differential biomass hydrolysis gives an insight into their interactions and clues about possible roles of novel hydrolytic and nonhydrolytic proteins in the synergistic deconstruction of lignocellulosic biomass. Our study thus provides the first comprehensive insight into the repertoire of proteins present in a high-performing secretome of a hypercellulolytic Penicillium funiculosum, their relative abundance in the secretome, and the interaction dynamics of the various protein groups in the secretome. The gleanings from the stoichiometry of these interactions hold a prospect as templates in the design of cost-effective synthetic cocktails for the optimal hydrolysis of biomass.
Synthetic Biology and Biofuels Group, ‡Malaria Group, and §DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology , Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Funso Emmanuel
FE
Ogunmolu
Synthetic Biology and Biofuels Group, ‡Malaria Group, and §DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology , Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Inderjeet
I
Kaur
Synthetic Biology and Biofuels Group, ‡Malaria Group, and §DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology , Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Mayank
M
Gupta
Synthetic Biology and Biofuels Group, ‡Malaria Group, and §DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology , Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Zeenat
Z
Bashir
Synthetic Biology and Biofuels Group, ‡Malaria Group, and §DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology , Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Nandita
N
Pasari
Synthetic Biology and Biofuels Group, ‡Malaria Group, and §DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology , Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Syed Shams
SS
Yazdani
D018533:Biomass; D044602:Cellulases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015870:Gene Expression; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D013058:Mass Spectrometry; D058977:Molecular Sequence Annotation; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D040901:Proteomics / Q000379:methods
D005656:Fungal Proteins; C036909:lignocellulose; D008031:Lignin; D044602:Cellulases
CAZymes; Penicillium funiculosum; lignocellulosic biomass; nonhydrolytic accessory proteins; quantitative mass spectrometry; saccharification; secretome