Diterpenoids from Saliva plebeia R. Br. and Their Antioxidant and Anti-Inflammatory Activities.
A new skeleton of diterpenoid, 1,2,3,4,4α,9,10,10α-octahydro-(4α-hydroxyymethyl) -1,1-dimethyl-9-(1-methylethyl)-(2S,3S,4αR,9R,10αS)-2,3,5,7-phenanthrenetertrol, named plebeianiol A (1), along with four known diterpenoids (2-5), were isolated from Salvia plebeia R. Br. Their structures were determined on the basis of spectral analysis. In the bioactivity tests, compounds 1, 2 and 5 showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities with IC50 values of 20.0-29.6 µM. In addition, these three compounds had significant inhibitory effects on reactive oxygen species (ROS) production in lipopolysaccharide (LPS)-induced macrophages. Compounds 1-3 inhibited nitric oxide (NO) production in LPS-induced macrophages with IC50 values of 18.0-23.6 µM. These results showed that compounds 1, 2 had significant antioxidant and anti-inflammatory activities and might provide basis for the treatment of diseases associated with oxidative lesions and inflammation.
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. zengbiao8839@163.com.
Bao-Bao
BB
Zhang
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. hbqnjfu@163.com.
Bai-Qiu
BQ
He
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. sjb_sp@163.com.
Jian-Bo
JB
Sun
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. zengbiao8839@163.com.
Biao
B
Zeng
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. xjsforever@sina.com.
Xiao-Ji
XJ
Shi
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. zhouyong941026@163.com.
Yong
Y
Zhou
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. yingniu13@aliyun.com.
Ying
Y
Niu
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. nie9993939@163.com.
Shi-Qi
SQ
Nie
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. fengsunlight@163.com.
Feng
F
Feng
Nanjing Sanhome Pharmaceutical Co., Ltd., Nanjing 210018, China. yanliang08@163.com.
Yan
Y
Liang
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. fhwu2000@cpu.edu.cn.
Fei-Hua
FH
Wu
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D053898:Biosynthetic Pathways / Q000187:drug effects; D001713:Biphenyl Compounds / Q000737:chemistry; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010851:Picrates / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D000067996:RAW 264.7 Cells; D027544:Salvia / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D004224:Diterpenes; D010851:Picrates; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl
Salvia plebeia; anti-inflammatory activity; antioxidant activity; diterpenoids