Trophoblast retrieval and isolation from the cervix (TRIC) is unaffected by early gestational age or maternal obesity.
OBJECTIVE
The objective of this study is to evaluate whether trophoblast yield obtained by trophoblast retrieval and isolation from the cervix (TRIC) is affected by pregnancy outcome, gestational age (GA) at retrieval, maternal body mass index (BMI), parity, or maternal age.


METHODS
TRIC was performed on 224 ongoing pregnancies between 5 and 20 weeks of GA. Trophoblast cells were isolated from cervical cells using anti-human leukocyte antigen-G antibody coupled to magnetic nanoparticles. Purity was assessed by the percentage of isolated cells that express β-hCG. Patient records were monitored until delivery, and pregnancy outcomes were determined. Trophoblast yield was compared with GA at time of collection, maternal BMI, parity, maternal age, and outcome of pregnancy, using linear regression.


RESULTS
There was no effect of GA, maternal BMI, parity, and maternal age on trophoblast yield. Trophoblast yield decreased significantly with early pregnancy loss compared with uncomplicated pregnancies that delivered at term. Trophoblast yield with preeclampsia or intrauterine growth restriction was decreased compared with healthy term outcomes; however, they did not reach statistical significance.


CONCLUSIONS
If TRIC becomes available as a method for non-invasive prenatal testing, our data demonstrate that it is unaffected by BMI and is useful as early as 5 weeks of GA.
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Rani
R
Fritz
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Hamid Reza
HR
Kohan-Ghadr
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Alex
A
Sacher
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Alan D
AD
Bolnick
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Brian A
BA
Kilburn
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Jay M
JM
Bolnick
Department of Obstetrics and Gynecology, Georgia Regents University, Augusta, GA, USA.
Michael P
MP
Diamond
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Sascha
S
Drewlo
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.|Program in Reproductive and Adult Endocrinology, NIH, NICHD, DHHS, Bethesda, MD, USA.
D Randall
DR
Armant
D000328:Adult; D005260:Female; D005865:Gestational Age; D006801:Humans; D009765:Obesity / Q000473:pathology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000473:pathology*; D011296:Prenatal Diagnosis / Q000379:methods*; D011446:Prospective Studies; D014327:Trophoblasts / Q000473:pathology*; D055815:Young Adult