Detection of Antibodies against Human and Plant Aquaporins in Patients with Multiple Sclerosis.
Multiple sclerosis (MS) is an autoimmune disease that affects the body's central nervous system. Around 90% of MS sufferers are diagnosed with relapsing-remitting MS (RRMS). We used ELISA to measure IgG, IgA, and IgM antibodies against linear epitopes of human and plant aquaporins (AQP4) as well as neural antigens in RRMS patients and controls to determine whether patients suffering from RRMS have simultaneous elevations in antibodies against these peptides and antigens. In comparison to controls, significant elevations in isotype-specific antibodies against human and plant AQP4 and neural antigens such as MBP, MOG, and S100B were detected in RRMS patients, indicating a high correlation in antibody reaction between plant aquaporins and brain antigens. This correlation between the reactivities of RRMS patients with various tested antigens was the most significant for the IgM isotype. We conclude that a subclass of patients with RRMS reacts to both plant and human AQP4 peptides. This immune reaction against different plant aquaporins may help in the development of dietary modifications for patients with MS and other neuroimmune disorders.
Immunosciences Lab., Inc., 822 S. Robertson Boulevard, Suite 312, Los Angeles, CA 90035, USA ; Department of Preventive Medicine, Loma Linda University, 24785 Stewart Street, Loma Linda, CA 92354, USA.
Aristo
A
Vojdani
Department of Mathematics, Boise State University, 1910 University Drive, Boise, ID 83725, USA.
Partha Sarathi
PS
Mukherjee
Immunosciences Lab., Inc., 822 S. Robertson Boulevard, Suite 312, Los Angeles, CA 90035, USA.
Joshua
J
Berookhim
Department of Preventive Medicine, Loma Linda University, 24785 Stewart Street, Loma Linda, CA 92354, USA ; Department of Clinical Sciences, Bastyr University California, 4106 Sorrento Valley Boulevard, San Diego, CA 92121, USA.
Datis
D
Kharrazian