Microbead Implantation in the Zebrafish Embryo.
The zebrafish has emerged as a valuable genetic model system for the study of developmental biology and disease. Zebrafish share a high degree of genomic conservation, as well as similarities in cellular, molecular, and physiological processes, with other vertebrates including humans. During early ontogeny, zebrafish embryos are optically transparent, allowing researchers to visualize the dynamics of organogenesis using a simple stereomicroscope. Microbead implantation is a method that enables tissue manipulation through the alteration of factors in local environments. This allows researchers to assay the effects of any number of signaling molecules of interest, such as secreted peptides, at specific spatial and temporal points within the developing embryo. Here, we detail a protocol for how to manipulate and implant beads during early zebrafish development.
Department of Biological Sciences, University of Notre Dame.
Gary F
GF
Gerlach
Department of Biological Sciences, University of Notre Dame.
Elvin E
EE
Morales
Department of Biological Sciences, University of Notre Dame; rwingert@nd.edu.
Rebecca A
RA
Wingert
D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000601:surgery; D047108:Embryonic Development; D005260:Female; D008297:Male; D008863:Microspheres; D015027:Zebrafish / Q000196:embryology* / Q000601:surgery*