Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. "2KEN8" mature pollen.
Proteomic analysis was used to generate a map of Populus deltoides CL. "2KEN8" mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy.
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China ; Research Institute of Wood Industry, Chinese Academy of Forestry Beijing, China.
Jin
J
Zhang
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China, University of Chinese Academy of Sciences Beijing, China.
Li-Shuan
LS
Wu
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
Wei
W
Fan
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
Xiao-Ling
XL
Zhang
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.
Hui-Xia
HX
Jia
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
Yu
Y
Li
Research Institute of Wood Industry, Chinese Academy of Forestry Beijing, China.
Ya-Fang
YF
Yin
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.
Jian-Jun
JJ
Hu
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.
Meng-Zhu
MZ
Lu
MALDI-TOF/TOF MS/MS; Populus deltoids; allergen; pollen; profilin; proteomics; two-dimensional gel electrophoresis