Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone.
In this study, species distribution and antimicrobial susceptibility of cultivated Pseudomonas spp. were studied in influent (INF), effluent (EFF), and marine outfall (MOut) of wastewater treatment plant (WWTP). The susceptibility was tested against 8 antimicrobial classes, active against Pseudomonas spp.: aminoglycosides, carbapenems, broad-spectrum cephalosporins from the 3rd and 4th generation, extended-spectrum penicillins, as well as their combination with the β-lactamase inhibitors, monobactams, fluoroquinolones, and polymyxins. Among identified species, resistance to all antimicrobials but colistin was shown by Pseudomonas putida, the predominant species in all sampling points. In other species, resistance was observed mainly against ceftazidime, ticarcillin, ticarcillin-clavulanate, and aztreonam, although some isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas pseudoalcaligenes, and Pseudomonas protegens showed multidrug-resistance (MDR) phenotype. Among P. putida, resistance to β-lactams and to fluoroquinolones as well as multidrug resistance become more prevalent after wastewater treatment, but the resistance rate decreased in marine water samples. Obtained data, however, suggests that Pseudomonas spp. are equipped or are able to acquire a wide range of antibiotic resistance mechanisms, and thus should be monitored as possible source of resistance genes.
Department of Water and Wastewater Technology, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Narutowicza 11/12, 80-233, Gdansk, Poland. ansob@pg.gda.pl.
Aneta
A
Luczkiewicz
Institute of Oceanology Polish Academy of Sciences, Powstancow Warszawy 55, 81-712, Sopot, Poland. ewachec@iopan.gda.pl.
Ewa
E
Kotlarska
Department of Water and Wastewater Technology, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Narutowicza 11/12, 80-233, Gdansk, Poland. wojartic@pg.gda.pl.
Wojciech
W
Artichowicz
Department of Water and Wastewater Technology, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Narutowicza 11/12, 80-233, Gdansk, Poland. kattaras@pg.gda.pl.
Katarzyna
K
Tarasewicz
Department of Water and Wastewater Technology, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Narutowicza 11/12, 80-233, Gdansk, Poland. sksiazek@pg.gda.pl.
Sylwia
S
Fudala-Ksiazek
D000890:Anti-Infective Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D024841:Fluoroquinolones / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009792:Oceans and Seas; D010802:Phylogeny; D011044:Poland; D011549:Pseudomonas / Q000187:drug effects* / Q000254:growth & development; D012623:Seawater / Q000737:chemistry / Q000382:microbiology*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D018508:Water Purification; D047090:beta-Lactams / Q000494:pharmacology
D000890:Anti-Infective Agents; D015780:Carbapenems; D003091:Colistin; D024841:Fluoroquinolones; D062065:Wastewater; D047090:beta-Lactams
Antimicrobial susceptibility; Marine outfall; Pseudomonas spp.; Species distribution; Wastewater