Cell-free expression of functional receptor tyrosine kinases.
Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.
University of California Davis School of Medicine, Radiation Oncology, Sacramento, California, USA.
Wei
W
He
University of California Davis School of Medicine, Internal Medicine, Division of Hematology Oncology, Sacramento, California, USA.
Tiffany M
TM
Scharadin
University of California Davis School of Medicine, Biochemistry and Molecular Medicine, Sacramento, California, USA.
Matthew
M
Saldana
University of California Davis School of Medicine, Internal Medicine, Division of Hematology Oncology, Sacramento, California, USA.
Candice
C
Gellner
University of California Davis School of Medicine, Radiation Oncology, Sacramento, California, USA.
Steven
S
Hoang-Phou
University of California Davis School of Medicine, Internal Medicine, Division of Hematology Oncology, Sacramento, California, USA.
Christina
C
Takanishi
1] Lawrence Berkeley National Laboratory, Berkeley, California, USA [2] University of California Santa Cruz, Chemistry and Biochemistry, Santa Cruz, California, USA.
Gregory L
GL
Hura
1] Lawrence Berkeley National Laboratory, Berkeley, California, USA [2] Scripps Research Institute, La Jolla, California, USA.
John A
JA
Tainer
1] University of California Davis School of Medicine, Biochemistry and Molecular Medicine, Sacramento, California, USA [2] University of California Davis Comprehensive Cancer Center, Sacramento, CA California, USA.
Kermit L
KL
Carraway
1] University of California Davis School of Medicine, Internal Medicine, Division of Hematology Oncology, Sacramento, California, USA [2] University of California Davis Comprehensive Cancer Center, Sacramento, CA California, USA.
Paul T
PT
Henderson
1] University of California Davis School of Medicine, Radiation Oncology, Sacramento, California, USA [2] University of California Davis Comprehensive Cancer Center, Sacramento, CA California, USA [3] Lawrence Livermore National Laboratory, Livermore, California, USA.
Matthew A
MA
Coleman
D002474:Cell-Free System; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*
D020794:Receptor Protein-Tyrosine Kinases