Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization.
Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France.
Marlène
M
Keravec
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France.
Jérôme
J
Mounier
Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA USA ; QIAGEN Marseille SA, Research & Development Luminy Biotech Enterprises, Marseille, France.
Emmanuel
E
Prestat
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France ; Department of Bacteriology-Virology, Hygiene and Parasitology-Mycology, University Hospital of Brest, Brest, France.
Sophie
S
Vallet
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, USA.
Janet K
JK
Jansson
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France.
Gaëtan
G
Burgaud
INSERM CIC 0502, University Hospital of Brest, Brest, France.
Sylvain
S
Rosec
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France.
Stéphanie
S
Gouriou
CF Center, Roscoff, France.
Gilles
G
Rault
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France.
Emmanuel
E
Coton
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France.
Georges
G
Barbier
EA 3882-Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Groupe de Bactériologie-Virologie, Faculté de Médecine et des Sciences de la Santé de Brest, University of Brest, 22 Avenue Camille Desmoulins, C.S. 93837, 29238 Brest Cedex 3, France ; Department of Bacteriology-Virology, Hygiene and Parasitology-Mycology, University Hospital of Brest, Brest, France.
Geneviève
G
Héry-Arnaud
Anaerobes; Cystic fibrosis; Early colonization; Pseudomonas aeruginosa; Respiratory tract microbiota; Respiratory viruses