Body Mass Parameters, Lipid Profiles and Protein Contents of Zebrafish Embryos and Effects of 2,4-Dinitrophenol Exposure.
Morphology and physiology of fish embryos undergo dramatic changes during their development until the onset of feeding, supplied only by endogenous yolk reserves. For obtaining an insight how these restructuring processes are reflected by body mass related parameters, dry weights (dw), contents of the elements carbon and nitrogen and lipid and protein levels were quantified in different stages within the first four days of embryo development of the zebrafish (Danio rerio). The data show age dependent changes in tissue composition. Dry weights decreased significantly from 79μgdw/egg at 0hours post fertilization (hpf) to 61 μgdw/egg after 96 hpf. The amounts of total carbon fluctuated between 460 mg g-1 and 540 mg g-1 dw, nitrogen was at about 100 mg g-1 dw and total fatty acids were between 48-73 mg g-1 dw. In contrast to these parameters that remained relatively constant, the protein content, which was 240 mg g-1 at 0 hpf, showed an overall increase of about 40%. Comparisons of intact eggs and dechorionated embryos at stages prior to hatching (24, 30, 48 hpf) showed that the differences seen for dry weight and for carbon and nitrogen contents became smaller at more advanced stages, consistent with transition of material from the chorion to embryo tissue. Further, we determined the effect of 2,4-dinitrophenol at a subacutely toxic concentration (14 μM, LC10) as a model chemical challenge on the examined body mass related parameters. The compound caused significant decreases in phospholipid and glycolipid fatty acid contents along with a decrease in the phospholipid fatty acid unsaturation index. No major changes were observed for the other examined parameters. Lipidomic studies as performed here may thus be useful for determining subacute effects of lipophilic organic compounds on lipid metabolism and on cellular membranes of zebrafish embryos.
UFZ-Helmholtz Centre for Environmental Research, Department of Environmental Biotechnology, Permoserstraße 15, 04318, Leipzig, Germany.
Nancy
N
Hachicho
UFZ-Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstraße 15, 04318, Leipzig, Germany.
Sarah
S
Reithel
UFZ-Helmholtz Centre for Environmental Research, Department of Environmental Biotechnology, Permoserstraße 15, 04318, Leipzig, Germany.
Anja
A
Miltner
UFZ-Helmholtz Centre for Environmental Research, Department of Environmental Biotechnology, Permoserstraße 15, 04318, Leipzig, Germany.
Hermann J
HJ
Heipieper
UFZ-Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstraße 15, 04318, Leipzig, Germany.
Eberhard
E
Küster
UFZ-Helmholtz Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstraße 15, 04318, Leipzig, Germany.
Till
T
Luckenbach
D019297:2,4-Dinitrophenol / Q000009:adverse effects*; D000818:Animals; D015992:Body Mass Index; D002244:Carbon / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002823:Chorion / Q000187:drug effects / Q000378:metabolism; D004531:Eggs; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism*; D047108:Embryonic Development / Q000187:drug effects*; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D009584:Nitrogen / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
D005227:Fatty Acids; D008055:Lipids; D011506:Proteins; D002244:Carbon; D009584:Nitrogen; D019297:2,4-Dinitrophenol