De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing.
Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of ~ 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Pavan Kumar
PK
Kakumani
Bionivid Technology Pvt. Ltd., 401, 4 AB Cross, 1st Main, Kasturi Nagar, NGEF East, Bangalore, 560043, India.
Rohit
R
Shukla
Bionivid Technology Pvt. Ltd., 401, 4 AB Cross, 1st Main, Kasturi Nagar, NGEF East, Bangalore, 560043, India.
Vivek N
VN
Todur
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India. pawanmal@gmail.com.
Pawan
P
Malhotra
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India. sunilm@icgeb.res.in.|Present address: Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India. sunilm@icgeb.res.in.
Sunil K
SK
Mukherjee
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India. raj@icgeb.res.in.
Raj K
RK
Bhatnagar
D000818:Animals; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D061987:Sf9 Cells; D018411:Spodoptera / Q000235:genetics*; D059467:Transcriptome
D019476:Insect Proteins