Calcium glycerophosphate preserves transepithelial integrity in the Caco-2 model of intestinal transport.
OBJECTIVE
To assess the direct effects of ischemia on intestinal epithelial integrity. Furthermore, clinical efforts at mitigating the effect of hypoperfusion on gut permeability have focused on restoring gut vascular function.


METHODS
We report that, in the Caco-2 cell model of transepithelial transport, calcium glycerophosphate (CGP), an inhibitor of intestinal alkaline phosphatase F3, has a significant effect to preserve transepithelial electrical resistance (TEER) and to attenuate increases in mannitol flux rates during hypoxia or cytokine stimulation.


RESULTS
The effect was observable even at concentrations as low as 1 μmol/L. As celiac disease is also marked by a loss of gut epithelial integrity, the effect of CGP to attenuate the effect of the α-gliadin peptide 31-55 was also examined. In this instance, CGP exerted little effect of preservation of TEER, but significantly attenuated peptide induced increase in mannitol flux.


CONCLUSIONS
It appears that CGP treatment might synergize with other therapies to preserve gut epithelial integrity.
Palika Datta, Margaret T Weis, Department of Biomedical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, United States.
Palika
P
Datta
Palika Datta, Margaret T Weis, Department of Biomedical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, United States.
Margaret T
MT
Weis
D001692:Biological Transport; D018938:Caco-2 Cells; D015687:Cell Hypoxia; D016207:Cytokines / Q000494:pharmacology; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005903:Gliadin / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology*; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D010539:Permeability; D013997:Time Factors
D016207:Cytokines; D005994:Glycerophosphates; D008353:Mannitol; D005903:Gliadin
Calcium glycerophosphate; Cytokine; Gliadin peptide; Intestinal ischemia; Intestinal permeability