Polymorphisms in GCKR, SLC17A1 and SLC22A12 were associated with phenotype gout in Han Chinese males: a case-control study.
BACKGROUND
Gout is a common arthritic disease resulting from elevated serum uric acid (SUA) level. A large meta-analysis including 28,141 individuals identified nine single nucleotide polymorphisms (SNPs) associated with altered SUA level in a Caucasian population. However, raised SUA level alone is not sufficient for the development of gout arthritis and most of these SNPs have not been studied in a Han Chinese population. Here, we performed a case-control association analysis to investigate the relationship between these SUA correlated SNPs and gout arthritis in Han Chinese.


METHODS
A total of 622 ascertained gout p9atients and 917 healthy controls were genotyped. Genome-wide significant SNPs, rs12129861, rs780094, rs734553, rs742132, rs1183201, rs12356193, rs17300741 and rs505802 in the previous SUA study, were selected for our analysis.


RESULTS
No deviation from the Hardy-Weinberg equilibrium was observed either in the case or control cohorts (corrected p > 0.05). Three SNPs, rs780094 (located in GCKR, corrected p = 1.78E(-4), OR = 0.723), rs1183201 (located in SLC17A1, corrected p = 1.39E(-7), OR = 0.572) and rs505802 (located in SLC22A12, corrected p = 0.007, OR = 0.747), were significantly associated with gout on allelic level independent of potential cofounding traits. While the remaining SNPs were not replicated. We also found significant associations of uric acid concentrations with these three SNPs (rs780094 in GCKR, corrected p = 3.94E(-5); rs1183201 in SLC17A1, corrected p = 0.005; rs505802 in SLC22A12, corrected p = 0.003) and of triglycerides with rs780094 (located in GCKR, corrected p = 2.96E(-4)). Unfortunately, SNP-SNP interactions for these three significant SNPs were not detected (rs780094 vs rs1183201, p = 0.402; rs780094 vs rs505802, p = 0.434; rs1183201 vs rs505802, p = 0.143).


CONCLUSIONS
Three SUA correlated SNPs in Caucasian population, rs780094 in GCKR, rs1183201 in SLC17A1 and rs505802 in SLC22A12 were confirmed to be associated with gout arthritis and uric acid concentrations in Han Chinese males. Considering genetic differences among populations and complicated pathogenesis of gout arthritis, more validating tests in independent populations and relevant functional experiments are suggested in future.
Shandong Gout Clinical Medical Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. zhouzhaoweiqd@hotmail.com.|Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. zhouzhaoweiqd@hotmail.com.
Zhao-Wei
ZW
Zhou
Shandong Gout Clinical Medical Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. cuillqd@163.com.|Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. cuillqd@163.com.
Ling-Ling
LL
Cui
Shandong Gout Clinical Medical Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. hanlinqd@126.com.|Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. hanlinqd@126.com.
Lin
L
Han
Shandong Gout Clinical Medical Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. wangcanabc@126.com.|Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. wangcanabc@126.com.
Can
C
Wang
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200030, China. zhijiansong@126.com.
Zhi-Jian
ZJ
Song
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200030, China. celaoforever@hotmail.com.
Jia-Wei
JW
Shen
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200030, China. lizqsjtu@163.com.
Zhi-Qiang
ZQ
Li
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200030, China. chenjhv@hotmail.com.
Jian-Hua
JH
Chen
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200030, China. wenzujia@126.com.
Zu-Jia
ZJ
Wen
Shandong Gout Clinical Medical Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. wangxiaominqd@163.com.|Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. wangxiaominqd@163.com.
Xiao-Min
XM
Wang
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200030, China. yongyongshi@gmail.com.
Yong-Yong
YY
Shi
Shandong Gout Clinical Medical Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. licgqd@163.com.|Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China. licgqd@163.com.
Chang-Gui
CG
Li
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D060005:Genotyping Techniques; D006073:Gout / Q000208:ethnology / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D027361:Organic Anion Transporters / Q000235:genetics*; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D050609:Sodium-Phosphate Cotransporter Proteins, Type I / Q000235:genetics*
D048868:Adaptor Proteins, Signal Transducing; C485100:GCKR protein, human; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; C493546:SLC17A1 protein, human; C491241:SLC22A12 protein, human; D050609:Sodium-Phosphate Cotransporter Proteins, Type I