Association of a single nucleotide polymorphism in the 5' upstream region of the porcine myosin heavy chain 4 gene with meat quality traits in pigs.
We identified a potential molecular marker associated with meat quality traits in the myosin heavy chain 4, MYH4 gene of Landrace pigs. Sequencing revealed a single nucleotide polymorphism (SNP; g.-1398G>T) in the 5' upstream region of MYH4. It was significantly associated with the number of type IIa muscle fibers and water-holding capacity based on filter-paper fluid uptake. The GG genotype groups had a greater number of type IIa fibers and a larger area composed of type IIa fibers than the other genotype group (P = 0.004 and P = 0.061, respectively). Expression level of MYH4 gene in the genotype TT or GT was higher than in genotype of GG (P < 0.0001). The T allele may enhance expression level of MYH4 gene and then the portion of IIb type fiber in the muscle be increased by the T allelle. Therefore, we suggest that the g.-1398G>T in the 5' upstream region of the porcine MYH4 may be used as a molecular marker for meat quality traits, although its functional effect is not defined yet.
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Korea.
Eun-Seok
ES
Cho
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Korea.
Kyung-Tai
KT
Lee
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Jun-Mo
JM
Kim
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Korea.
Si-Woo
SW
Lee
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Korea.
Hyeon-Jeong
HJ
Jeon
Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang, Korea.
Seung-Hwan
SH
Lee
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Ki-Chang
KC
Hong
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Suwon, Korea.
Tae-Hun
TH
Kim
D000483:Alleles; D000818:Animals; D001834:Body Water / Q000378:metabolism; D063427:Food Quality; D015870:Gene Expression; D056726:Genetic Association Studies / Q000662:veterinary*; D005819:Genetic Markers; D005838:Genotype; D008460:Meat; D018485:Muscle Fibers, Skeletal / Q000145:classification / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013552:Swine / Q000235:genetics* / Q000378:metabolism*
D005819:Genetic Markers; D018995:Myosin Heavy Chains
meat quality; muscle fiber composition; myosin heavy chain 4 gene; pig; single nucleotide polymorphism