Diurnal gene expression of lipolytic natriuretic peptide receptors in white adipose tissue.
Disruption of the circadian rhythm can lead to obesity and cardiovascular disease. In white adipose tissue, activation of the natriuretic peptide receptors (NPRs) stimulates lipolysis. We have previously shown that natriuretic peptides are expressed in a circadian manner in the heart, but the temporal expression profile of their cognate receptors has not been examined in white adipose tissue. We therefore collected peri-renal white adipose tissue and serum from WT mice. Tissue mRNA contents of NPRs - NPR-A and NPR-C, the clock genes Per1 and Bmal1, and transcripts involved in lipid metabolism were quantified at 4-h intervals: in the diurnal study, mice were exposed to a period of 12 h light followed by 12 h darkness (n=52). In the circadian study, mice were kept in darkness for 24 h (n=47). Concomitant serum concentrations of free fatty acids, glycerol, triglycerides (TGs), and insulin were measured. Per1 and Bmal1 mRNA contents showed reciprocal circadian profiles (P<0.0001). NPR-A mRNA contents followed a temporal pattern (P=0.01), peaking in the dark (active) period. In contrast, NPR-C mRNA was expressed in an antiphase manner with nadir in the active period (P=0.007). TG concentrations in serum peaked in the active dark period (P=0.003). In conclusion, NPR-A and NPR-C gene expression is associated with the expression of clock genes in white adipose tissue. The reciprocal expression may thus contribute to regulate lipolysis and energy homeostasis in a diurnal manner.
Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark.
Julie
J
Smith
Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark.
Jan
J
Fahrenkrug
Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark.
Henrik L
HL
Jørgensen
Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark.
Christina
C
Christoffersen
Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark Department of Clinical Biochemistry (KB3014) Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark Department of Technology Faculty of Health and Technology, Metropolitan University College, Copenhagen, Denmark Department of Clinical Biochemistry Faculty of Health Sciences, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark Department of Biomedical Sciences Copenhagen University, Copenhagen, Denmark Department of Clinical Medicine Aarhus University, Aarhus, Denmark JPG@dadlnet.dk.
Jens P
JP
Goetze
ANP; BNP; adipocytes; circadian; diurnal; lipolysis; receptor