Apoptosis in chronic cutaneous lupus erythematosus, discoid lupus, and lupus profundus.
BACKGROUND
Lupus erythematosus is a multisystemic disease that is characterized by autoantibody production and immune complex deposition in such tissues as the mucosa, joints, the central nervous system, and skin. Cutaneous lupus erythematosus is categorized as acute, subacute, and chronic. Chronic cutaneous lupus erythematosus comprises discoid lupus erythematosus (DLE) and lupus profundus (LP).


OBJECTIVE
To analyze the expression of proapoptotic molecules in patients with lupus erythematosus discoid and lupus profundus.


METHODS
Descriptive study, the study groups comprised 10 cases of LP and 10 cases of DLE, and a control. Skin samples of cases and controls were processed for immunohistochemistry and by TUNEL technique. The database and statistical analysis was performed (statistical test X(2)) SPSS (Chicago, IL, USA).


RESULTS
Apoptotic features were broadly distributed along the skin biopsies in epidermal keratinocytes as well as at dermis. By immunohistochemistry the expression of Fas receptor and Fas-L was higher in the skin of lupus patients compared with controls. We also noted differences in Fas-L, -Fas, and -Bax proteins expression intensity in discoid lupus erythematosus patients in the epidermis, and hair follicles.


CONCLUSIONS
Fas and Fas-L are expressed similarly in LP and DLE.
Department of Dermatology, Dr. Manuel Gea González General Hospital México City, Mexico.
Claudia Ileana
CI
Sáenz-Corral
Department of Dermatology, Dr. Manuel Gea González General Hospital México City, Mexico.
María Elisa
ME
Vega-Memíje
Department of Dermatology, Dr. Manuel Gea González General Hospital México City, Mexico.
Eduwiges
E
Martínez-Luna
Pathology Laboratory, Faculty of Dentistry, Juarez University of The State of Durango Durango, Dgo, Mexico.
Juan Carlos
JC
Cuevas-González
Professor of Medical School ITESM Campus Guadalajara Mexico. Private Practice (Dermocare).
Alma Angélica
AA
Rodríguez-Carreón
Laboratory of Immunology and Molecular Biology, UABE, Universidad Autónoma de Zacatecas Zacatecas, Zac, Mexico.
Juan José Bollain-Y-Goytia
JJ
de la Rosa
Laboratory of Immunology and Molecular Biology, UABE, Universidad Autónoma de Zacatecas Zacatecas, Zac, Mexico.
Felipe de Jesús Torres
Fde J
Del Muro
Laboratory of Immunology and Molecular Biology, UABE, Universidad Autónoma de Zacatecas Zacatecas, Zac, Mexico.
Esperanza
E
Avalos-Díaz
D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D053222:Fas Ligand Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008178:Lupus Erythematosus, Cutaneous / Q000378:metabolism / Q000473:pathology*; D008179:Lupus Erythematosus, Discoid / Q000378:metabolism / Q000473:pathology*; D015435:Panniculitis, Lupus Erythematosus / Q000378:metabolism / Q000473:pathology*; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000032:analysis; D019014:fas Receptor / Q000032:analysis
C494826:BAX protein, human; D015415:Biomarkers; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D051028:bcl-2-Associated X Protein; D019014:fas Receptor
Apoptosis; chronic cutaneous lupus erythematosus; discoid lupus; lupus profundus; proapoptotic molecules