Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice.
The goal of this study is to investigate the feasibility of using CD81- (Cluster of Differentiation 81 protein-) targeted microparticles of iron oxide (CD81-MPIO) for magnetic resonance imaging (MRI) of the murine atherosclerosis. CD81-MPIO and IgG- (Immunoglobulin G-) MPIO were prepared by covalently conjugating, respectively, with anti-CD81 monoclonal and IgG antibodies to the surface of the tosyl activated MPIO. The relevant binding capability of the MPIO was examined by incubating them with murine bEnd.3 cells stimulated with phenazine methosulfate (PMS) and its effect in shortening T2 relaxation time was also examined. MRI in apolipoprotein E-deficient mice was studied in vivo. Our results show that CD81-MPIO, but not IgG-MPIO, can bind to the PMS-stimulated bEnd.3 cells. The T2 relaxation time was significantly shortened for stimulated bEnd.3 cells when compared with IgG-MPIO. In vivo MRI in apolipoprotein E-deficient mice showed highly conspicuous areas of low signal after CD81-MPIO injection. Quantitative analysis of the area of CD81-MPIO contrast effects showed 8.96- and 6.98-fold increase in comparison with IgG-MPIO or plain MPIO, respectively (P < 0.01). Histological assay confirmed the expression of CD81 and CD81-MPIO binding onto atherosclerotic lesions. In conclusion, CD81-MPIO allows molecular assessment of murine atherosclerotic lesions by magnetic resonance imaging.
The Third Affiliated Hospital of Southern Medical University, Guangzhou 510500, China ; Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China ; Shenzhen Key Laboratory for Molecular Imaging, Shenzhen 518055, China.
Fei
F
Yan
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Wei
W
Yang
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Xiang
X
Li
The Third Affiliated Hospital of Southern Medical University, Guangzhou 510500, China.
Hongmei
H
Liu
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Xiang
X
Nan
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Lisi
L
Xie
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Dongliang
D
Zhou
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Guoxi
G
Xie
Department of Physics, University of Vermont, Burlington, VT 05405, USA.
Junru
J
Wu
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Bensheng
B
Qiu
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Xin
X
Liu
The Third Affiliated Hospital of Southern Medical University, Guangzhou 510500, China ; Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Hairong
H
Zheng
D000818:Animals; D050197:Atherosclerosis / Q000000981:diagnostic imaging* / Q000473:pathology; D055252:Cell-Derived Microparticles / Q000737:chemistry; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry; D005290:Ferric Compounds / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D008773:Methylphenazonium Methosulfate / Q000737:chemistry; D051379:Mice; D011859:Radiography; D060225:Tetraspanin 28 / Q000008:administration & dosage* / Q000737:chemistry
D003287:Contrast Media; D005290:Ferric Compounds; D007074:Immunoglobulin G; D060225:Tetraspanin 28; C000499:ferric oxide; D008773:Methylphenazonium Methosulfate