Hybrid use of combined and sequential delivery of growth factors and ultrasound stimulation in porous multilayer composite scaffolds to promote both vascularization and bone formation in bone tissue engineering.
In this study, a multilayer coating technology would be adopted to prepare a porous composite scaffold and the growth factor release and ultrasound techniques were introduced into bone tissue engineering to finally solve the problems of vascularization and bone formation in the scaffold whilst the designed multilayer composite with gradient degradation characteristics in the space was used to match the new bone growth process better. The results of animal experiments showed that the use of low intensity pulsed ultrasound (LIPUS) combined with growth factors demonstrated excellent capabilities and advantages in both vascularization and new bone formation in bone tissue engineering. The degradation of the used scaffold materials could match new bone formation very well. The results also showed that only RGD-promoted cell adhesion was insufficient to satisfy the needs of new bone formation while growth factors and LIPUS stimulation were the key factors in new bone formation.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Haoran
H
Yan
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Xia
X
Liu
Sichuan Centre for Disease Control and Prevention, Chengdu, 610041, People's Republic of China.
Minghua
M
Zhu
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Guilin
G
Luo
Sichuan Centre for Disease Control and Prevention, Chengdu, 610041, People's Republic of China.
Tao
T
Sun
Sichuan Centre for Disease Control and Prevention, Chengdu, 610041, People's Republic of China.
Qiang
Q
Peng
Sichuan Centre for Disease Control and Prevention, Chengdu, 610041, People's Republic of China.
Yi
Y
Zeng
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Taijun
T
Chen
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Yingying
Y
Wang
Sichuan Centre for Disease Control and Prevention, Chengdu, 610041, People's Republic of China.
Keliang
K
Liu
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Bo
B
Feng
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Jie
J
Weng
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Jianxin
J
Wang
D000818:Animals; D001842:Bone and Bones / Q000187:drug effects*; D020007:Implants, Experimental; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014465:Ultrasonics
D036341:Intercellular Signaling Peptides and Proteins
LIPUS; bone formation; bone tissue engineering; multilayer composite scaffolds; vascularization