Establishment and identification of human primary lung cancer cell culture in vitro.
OBJECTIVE
To explore a simple and practical method for human primary lung cancer cells culture in vitro.


METHODS
Tumor specimens from 6 lung cancer patients were isolated with collagenase digestion cultured in vitro. Then the characteristics of these cells were analyzed and identified by optical microscope observation, hematoxylin-eosin staining, immunocytochemistry, immunohistochemistry and tumor nude mice inoculation experiments, respectively.


RESULTS
Except for the small cell lung cancer, the other 5 samples were successfully isolated and cultured. The cultured cells showed typical characteristics of malignant cells and positive for cytokeratin 7 and 19. Moreover, the cancer cells readily formed subcutaneous tumors in nude mice and the pathological images of the transplanted tumor were consistent with its tumor origin.


CONCLUSIONS
The primary culture for human lung cancer cells can be successfully achieved with the method of collagenase digestion.
Department of Thoracic Oncology, Zhongnan Hospital of Wuhan University, Wuhan University Wuhan, Hubei 430071, China ; Department of People's Hospital of Beijing Daxing District Beijing 102600, China.
Lian-Lian
LL
Si
Department of Thoracic Oncology, Zhongnan Hospital of Wuhan University, Wuhan University Wuhan, Hubei 430071, China ; Hubei Cancer Clinical Study Center & Hubei Key Laboratory of Tumor Biological Behaviors Wuhan, Hubei 430071, China.
Lu
L
Lv
Medical Center for Human Reproduction, Beijing Chaoyang Hospital, Capital Medical University Beijing 100020, China.
Wen-Hui
WH
Zhou
Department of Thoracic Oncology, Zhongnan Hospital of Wuhan University, Wuhan University Wuhan, Hubei 430071, China ; Hubei Cancer Clinical Study Center & Hubei Key Laboratory of Tumor Biological Behaviors Wuhan, Hubei 430071, China.
Wei-Dong
WD
Hu
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D018196:Carcinoma, Adenosquamous / Q000378:metabolism / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D017364:Collagenases / Q000378:metabolism; D005260:Female; D006801:Humans; D053539:Keratin-19 / Q000378:metabolism; D053552:Keratin-7 / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D061251:Primary Cell Culture; D055752:Small Cell Lung Carcinoma / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D047368:Tumor Burden; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; C507497:KRT7 protein, human; D053539:Keratin-19; D053552:Keratin-7; D017364:Collagenases
H-E staining; Lung cancer; immunohistochemistry; morphology; primary cell culture