Levetiracetam ameliorates ovarian function in streptozotocin-induced diabetic rats.
Diabetes mellitus can adversely affect gonadal function. In the present study, we aimed to investigate the protective effects and mechanism of action of levetiracetam (LEV) on the ovaries in a streptozotocin (STZ)-induced diabetes model in rats. Twenty-one adult female rats were assigned to three groups as control, diabetes group treated with 1 mL/kg/d saline (STZ + SP) and diabetes group treated with 600 mg/kg/d LEV (STZ + LEV). Following 4 weeks treatment, blood samples were collected for biochemical analysis and ovariectomy was performed for histopathological examination. Plasma anti-Mullerian hormone (AMH), glutathione and total anti-oxidant capacity values were significantly lower whereas lipid peroxides and transforming growth factor-β (TGF-β) values were significantly higher in STZ + SP group compared to control. LEV treatment successfully decreased lipid peroxidation and TGF-β levels, and also increased anti-oxidant parameters and AMH levels in diabetic rats. Saline-treated rats significantly displayed ovarian degeneration and decreased counts of follicles. However, treatment of diabetic rats with LEV effectively prevented the degenerative changes and follicle loss. Also, LEV suppressed ovarian nuclear factor-kappa B (NF-kB) immunoexpression in diabetic rats. Taken together, we propose that LEV can ameliorate the adverse effects of diabetes on ovarian function via decreasing NF-kB expression and oxidative stress and increasing anti-oxidant status in rats.
a Department of Obstetrics and Gynecology , Ege University Medical School , Izmir , Turkey .|b Department of Stem Cell , Ege University, Institute of Health Sciences , Izmir , Turkey .
Levent
L
Akman
c Department of Physiology , and.
Oytun
O
Erbas
a Department of Obstetrics and Gynecology , Ege University Medical School , Izmir , Turkey .|b Department of Stem Cell , Ege University, Institute of Health Sciences , Izmir , Turkey .
Ali
A
Akdemir
d Department of Histology and Embryology , Ege University Medical School , Izmir , Turkey.
Altug
A
Yavasoglu
c Department of Physiology , and.
Dilek
D
Taskiran
a Department of Obstetrics and Gynecology , Ege University Medical School , Izmir , Turkey .
Mert
M
Kazandi
D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood; D001786:Blood Glucose; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D005260:Female; D005978:Glutathione / Q000097:blood; D000077287:Levetiracetam; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D010053:Ovary / Q000187:drug effects* / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects*; D010889:Piracetam / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
D001786:Blood Glucose; D000077287:Levetiracetam; D008315:Malondialdehyde; D013311:Streptozocin; D054304:Anti-Mullerian Hormone; D005978:Glutathione; D010889:Piracetam
Diabetes mellitus; NF-kB; TGF-β; levetiracetam; ovarian function