Analytical methods in sphingolipidomics: Quantitative and profiling approaches in food analysis.
In recent years, sphingolipidomics has emerged as an interesting omic science that encompasses the study of the full sphingolipidome characterization, content, structure and activity in cells, tissues or organisms. Like other omics, it has the potential to impact biomarker discovery, drug development and systems biology knowledge. Concretely, dietary food sphingolipids have gained considerable importance due to their extensively reported bioactivity. Because of the complexity of this lipid family and their diversity among foods, powerful analytical methodologies are needed for their study. The analytical tools developed in the past have been improved with the enormous advances made in recent years in mass spectrometry (MS) and chromatography, which allow the convenient and sensitive identification and quantitation of sphingolipid classes and form the basis of current sphingolipidomics methodologies. In addition, novel hyphenated nuclear magnetic resonance (NMR) strategies, new ionization strategies, and MS imaging are outlined as promising technologies to shape the future of sphingolipid analyses. This review traces the analytical methods of sphingolipidomics in food analysis concerning sample extraction, chromatographic separation, the identification and quantification of sphingolipids by MS and their structural elucidation by NMR.
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain.
Núria
N
Canela
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain.
Pol
P
Herrero
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain.
Sílvia
S
Mariné
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain.
Pedro
P
Nadal
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain.
Maria Rosa
MR
Ras
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain.
Miguel Ángel
MÁ
Rodríguez
Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Spain. Electronic address: lluis.arola@urv.cat.
Lluís
L
Arola
D005504:Food Analysis / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry; D013107:Sphingolipids / Q000032:analysis*
D008055:Lipids; D013107:Sphingolipids
Food; Liquid chromatography; Mass spectrometry; Nuclear magnetic resonance; Sphingolipid; Thin-layer chromatography