Mapping the Ultrafast Dynamics of Adenine onto Its Nucleotide and Oligonucleotides by Time-Resolved Photoelectron Imaging.
The intrinsic photophysics of nucleobases and nucleotides following UV absorption presents a key reductionist step toward understanding the complex photodamage mechanisms occurring in DNA. The decay mechanism of adenine in particular has been the focus of intense investigation, as has how these correlate to those of its more biologically relevant nucleotide and oligonucleotides in aqueous solution. Here, we report on time-resolved photoelectron imaging of the deprotonated 3'-deoxy-adenosine-5'-monophosphate nucleotide and the adenosine di- and trinucleotides. Through a comparison of gas- and solution-phase experiments and available theoretical studies, the dynamics of the base are shown to be relatively insensitive to the surrounding environment. The decay mechanism primarily involves internal conversion from the initially populated (1)ππ* states to the ground state. The relaxation dynamics of the adenosine oligonucleotides are similar to those of the nucleobase, in contrast to the aqueous oligonucleotides, where a fraction of the ensemble forms long-lived excimer states.
†Department of Chemistry, University of Durham, Durham DH1 3LE, United Kingdom.|‡Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
Adam S
AS
Chatterley
†Department of Chemistry, University of Durham, Durham DH1 3LE, United Kingdom.
Christopher W
CW
West
‡Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
Gareth M
GM
Roberts
‡Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
Vasilios G
VG
Stavros
†Department of Chemistry, University of Durham, Durham DH1 3LE, United Kingdom.
Jan R R
JR
Verlet
DNA damage; anion photoelectron spectroscopy; conical intersections; excited-state dynamics; nonradiative decay; photophysics