Investigation of metabolic changes in STZ-induced diabetic rats with hyperpolarized [1-13C]acetate.
In the metabolism of acetate several enzymes are involved, which play an important role in free fatty acid oxidation. Fatty acid metabolism is altered in diabetes patients and therefore acetate might serve as a marker for pathological changes in the fuel selection of cells, as these changes occur in diabetes patients. Acetylcarnitine is a metabolic product of acetate, which enables its transport into the mitochondria for energy production. This study investigates whether the ratio of acetylcarnitine to acetate, measured by noninvasive hyperpolarized [1-(13)C]acetate magnetic resonance spectroscopy, could serve as a marker for myocardial, hepatic, and renal metabolic changes in rats with Streptozotocin (STZ)-induced diabetes in vivo. We demonstrate that the conversion of acetate to acetylcarnitine could be detected and quantified in all three organs of interest. More interestingly, we found that the hyperpolarized acetylcarnitine to acetate ratio was independent of blood glucose levels and prolonged hyperglycemia following diabetes induction in a type-1 diabetes model.
Institute of Medical Engineering, Technische Universität München, Munich, Germany ulrich.koellisch@tum.de.
Ulrich
U
Koellisch
Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Christoffer
C
Laustsen
Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Thomas S
TS
Nørlinger
Department of Clinical Medicine, Aarhus University, Aarhus, Denmark Department of Endocrinology and Internal medicine, Aarhus University Hospital, Aarhus, Denmark Danish Diabetes Academy, Aarhus, Denmark.
Jakob Appel
JA
Østergaard
Department of Clinical Medicine, Aarhus University, Aarhus, Denmark Department of Endocrinology and Internal medicine, Aarhus University Hospital, Aarhus, Denmark.
Allan
A
Flyvbjerg
Nuklearmedizinische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
Concetta V
CV
Gringeri
GE Global Research, Munich, Germany.
Marion I
MI
Menzel
GE Global Research, Munich, Germany.
Rolf F
RF
Schulte
Institute of Medical Engineering, Technische Universität München, Munich, Germany.
Axel
A
Haase
Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Hans
H
Stødkilde-Jørgensen
Acetate; diabetes mellitus; hyperpolarization