Association and gene-gene interactions study of reelin signaling pathway related genes with autism in the Han Chinese population.
Autism is a neurodevelopmental disorder with unclear etiology. Reelin had been proposed to participate in the etiology of autism due to its important role in brain development. The goal of this study was to explore the association and gene-gene interactions of reelin signaling pathway related genes (RELN, VLDLR, LRP8, DAB1, FYN, and CDK5) with autism in Han Chinese population. Genotyping data of the six genes were obtained from a recent genome-wide association study performed in 430 autistic children who fulfilled the DSM-IV-TR criteria for autistic disorder, and 1,074 healthy controls. Single marker case-control association analysis and haplotype case-control association analysis were conducted after the data was screened. Multifactor dimensionality reduction (MDR) was applied to further test gene-gene interactions. Neither the single marker nor the haplotype association tests found any significant difference between the autistic group and the control group after permutation test of 1,000 rounds. The 4-locus MDR model (comprising rs6143734, rs1858782, rs634500, and rs1924267 which belong to RELN and DAB1) was determined to be the model with the highest cross-validation consistency (CVC) and testing balanced accuracy. The results indicate that an interaction between RELN and DAB1 may increase the risk of autism in the Han Chinese population. Furthermore, it can also be inferred that the involvement of RELN in the etiology of autism would occur through interaction with DAB1.
Institute of Mental Health, the Second Xiangya Hospital, Central South University, Changsha, China.
Yidong
Y
Shen
Institute of Mental Health, the Second Xiangya Hospital, Central South University, Changsha, China.|Mental Health Center of Shandong Province, Jinan, China.
Guanglei
G
Xun
Institute of Mental Health, the Second Xiangya Hospital, Central South University, Changsha, China.|State Key Laboratory of Medical Genetics, Central South University, Changsha, China.
Hui
H
Guo
Institute of Mental Health, the Second Xiangya Hospital, Central South University, Changsha, China.|The Second Affiliated Hospital of Xinxiang Medical College, Xinxiang, China.
Yiqun
Y
He
Institute of Mental Health, the Second Xiangya Hospital, Central South University, Changsha, China.
Jianjun
J
Ou
Institute of Mental Health, the Second Xiangya Hospital, Central South University, Changsha, China.
Huixi
H
Dong
State Key Laboratory of Medical Genetics, Central South University, Changsha, China.
Kun
K
Xia
State Key Laboratory of Medical Genetics, Central South University, Changsha, China.
Jingping
J
Zhao
D044466:Asian People / Q000235:genetics*; D001321:Autistic Disorder / Q000235:genetics*; D016022:Case-Control Studies; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002675:Child, Preschool; D002681:China; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D055106:Genome-Wide Association Study / Q000379:methods*; D006801:Humans; D008297:Male; D058011:Multifactor Dimensionality Reduction; D009419:Nerve Tissue Proteins / Q000235:genetics*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C000717127:RELN protein, human; D012697:Serine Endopeptidases
autism; interaction; polymorphism; reelin; signaling pathway