[Transforming growth factor-β1 induces epithelial-mesenchymal transition in A549 cells by up-regulating expression of calpain].
OBJECTIVE
To investigate the effect of transforming growth factor-β1 (TGF-β1) on epithelial-mesenchymal transition in type II alveolar epithelial cells and the relationship with Calpain.


METHODS
The type II alveolar epithelial cell line-A549 cells cultured in vitro were divided into 4 groups: the control group, the TGF-βl group, the TGF-β1 + PD150606 group, the PD150606 group. Given the corresponding processing,Western-blot and RT-PCR were utilized to evaluate the expression of Calpain- 1,E-cad and α-SMA.


RESULTS
Compared with the control group, the protein and mRNA expression of Calpain-1 and α-SMA in the TGF-β1 group was markedly increased, but the protein and mRNA expression of E-cad was significantly decreased. While, after calpain inhibitor PD150606 was added, the protein and mRNA expression of Calpain-1 and α-SMA was markedly decreased and the protein and mRNA expression of E-cad was significantly increased in the TGF-βl + PD150606 group, but there was no difference between TGF-β1 + PD150606 group and control group. But with Calpain inhibitor PD150606 alone, the expression of Calpain-1,E-cad and α-SMA was no difference with the control group.


CONCLUSIONS
TGF-βl could induce epithelial-mesenchymal transition in A549 cells by up-regulating expression of calpain which could be inhibited by calpain inhibitor PD150606.
Tan
T
Weijun
Cheng
C
Zhenshun
Tan
T
Qiuyue
Wang
W
Ting
Lian
L
Min
Zhang
Z
Li
D000179:Acrylates / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology*; D058750:Epithelial-Mesenchymal Transition; D005786:Gene Expression Regulation; D006801:Humans; D015533:Transcriptional Activation; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D015291:Transforming Growth Factors
C541116:ACTA2 protein, human; D000179:Acrylates; D000199:Actins; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C100442:PD 150606; D053773:Transforming Growth Factor beta1; D015291:Transforming Growth Factors; D002154:Calpain