Astaxanthin Normalizes Epigenetic Modifications of Bovine Somatic Cell Cloned Embryos and Decreases the Generation of Lipid Peroxidation.
Astaxanthin is an extremely common antioxidant scavenging reactive oxygen species (ROS) and blocking lipid peroxidation. This study was conducted to investigate the effects of astaxanthin supplementation on oocyte maturation, and development of bovine somatic cell nuclear transfer (SCNT) embryos. Cumulus-oocyte complexes were cultured in maturation medium with astaxanthin (0, 0.5, 1.0, or 1.5 mg/l), respectively. We found that 0.5 mg/l astaxanthin supplementation significantly increased the proportion of oocyte maturation. Oocytes cultured in 0.5 mg/l astaxanthin supplementation were used to construct SCNT embryos and further cultured with 0, 0.5, 1.0 or 1.5 mg/l astaxanthin. The results showed that the supplementation of 0.5 mg/l astaxanthin significantly improved the proportions of cleavage and blastulation, as well as the total cell number in blastocysts compared with the control group, yet this influence was not concentration dependent. Chromosomal analyses revealed that more blastomeres showed a normal chromosomal complement in 0.5 mg/l astaxanthin treatment group, which was similar to that in IVF embryos. The methylation levels located on the exon 1 of the imprinted gene H19 and IGF2, pluripotent gene OCT4 were normalized, and global DNA methylation, H3K9 and H4K12 acetylation were also improved significantly, which was comparable to that in vitro fertilization (IVF) embryos. Moreover, we also found that astaxanthin supplementation significantly decreased the level of lipid peroxidation. Our findings showed that the supplementation of 0.5 mg/l astaxanthin to oocyte maturation medium and embryo culture medium improved oocyte maturation, SCNT embryo development, increased chromosomal stability and normalized the epigenetic modifications, as well as inhibited overproduction of lipid peroxidation.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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Li
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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Wu
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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Zhuo
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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QF
Mao
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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Lan
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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Y
Zhang
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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S
Hua
D000107:Acetylation; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D002452:Cell Count / Q000662:veterinary; D065150:Cellular Reprogramming / Q000187:drug effects; D019976:Cloning, Organism / Q000662:veterinary; D019175:DNA Methylation; D046149:Embryo Culture Techniques / Q000662:veterinary; D004622:Embryo, Mammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D044127:Epigenesis, Genetic / Q000187:drug effects*; D005307:Fertilization in Vitro; D006657:Histones / Q000378:metabolism; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D015227:Lipid Peroxidation / Q000187:drug effects*; D008969:Molecular Sequence Data; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D017421:Sequence Analysis / Q000662:veterinary; D024341:Xanthophylls / Q000494:pharmacology
D000975:Antioxidants; D006657:Histones; D024341:Xanthophylls; C005948:astaxanthine