[Infection and molecular characteristics of Giardia in clinical diarrheal patients].
OBJECTIVE
To initially understand the infection status and the molecular characteristics of Giardia in clinical diarrheal patients.


METHODS
A total of 95 stool samples were collected from the clinical diarrheal patients admitted in a hospital in Shanghai from May to July, 2014, and the Giardia cysts in the samples were examined by an optical microscope. Then the tpi gene of Giardia in the positive samples were amplified by using the nested-PCR method, and the PCR products were sequenced and analyzed by using BLAST, ClustalX 1.83, and the phylogenetic tree was drawn by using MEGA6.0 software.


RESULTS
Only one patient was infected with Giardia and the positive detection rate was 1.05%. The Giardia cysts in the fecal specimen were seen clearly under the microscope. Through the identification by PCR, the amplified fragment was about 530 bp, and the sequencing analysis indicated it was Giardia and which was further identified as assemblage B by drawing phylogenetic tree based on tpi gene. Meanwhile, the sequence had 100% homology with the reported sequence from huian (KF271445).


CONCLUSIONS
Giardia infection can occur in the clinical diarrheal patients. The study could provide more data for understanding the genetic characteristics of Giardia and the epidemiological study of giardiasis.
Hua
H
Liu
Yu-juan
YJ
Shen
Yu-mei
YM
Zhang
Bin
B
Wang
Hui
H
Liu
Jian-ping
JP
Cao
D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000469:parasitology*; D005260:Female; D005872:Giardia / Q000235:genetics*; D005873:Giardiasis / Q000150:complications; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged