Immobilization of Thermomyces lanuginosus Xylanase on Aluminum Hydroxide Particles Through Adsorption: Characterization of Immobilized Enzyme.
Xylanase plays important roles in a broad range of industrial production as a biocatalyst, and its applications commonly require immobilization on supports to enhance its stability. Aluminum hydroxide, a carrier material with high surface area, has the advantages of simple and low-cost preparation and resistance to biodegradation, and can be potentially used as a proper support for xylanase immobilization. In this work, xylanase from Thermomyces lanuginosus was immobilized on two types of aluminum hydroxide particles (gibbsite and amorphous Al(OH)3) through adsorption, and the properties of the adsorbed enzymes were studied. Both particles had considerable adsorptive capacity and affinity for xylanase. Xylanase retained 75% and 64% of the original catalytic activities after adsorption to gibbsite and amorphous Al(OH)3. Both the adsorptions improved pH and thermal stability, lowered activation energy, and extended lifespan of the immobilized enzyme, as compared with the free enzyme. Xylanase adsorbed on gibbsite and amorphous Al(OH)3 retained 71% and 64% of its initial activity, respectively, after being recycled five times. These results indicated that aluminum hydroxides served as good supports for xylanase immobilization. Therefore, the adsorption of xylanase on aluminum hydroxide particles has promising potential for practical production.
College of Resources and Environmental Sciences, Henan Agricultural University, Zhengzhou 450000, P.R. China.
Ying
Y
Jiang
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Soil and Fertilizer Bureau of Shandong Province, Shandong 253016, P.R. China.
Yue
Y
Wu
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. China.
Huixin
H
Li
D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D032641:Eurotiales / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014995:Xylosidases / Q000737:chemistry / Q000378:metabolism*
D004337:Drug Carriers; D004800:Enzymes, Immobilized; D000536:Aluminum Hydroxide; D014995:Xylosidases
Adsorption; Amorphous Al(OH)3; Catalytic performance; Gibbsite; Xylanase