Developmental Status: Impact of Short-Term Ischemia on Follicular Survival of Whole Ovarian Transplantation in a Rabbit Model.
Ischemia is the first mechanism that provokes the loss of follicles in ovarian grafts over the long term. In whole ovarian transplantation, it remains unknown, however, how changes in follicular development are influenced by short-term ischemia. Fresh whole ovarian orthotopic auto-transplantation was performed in rabbits with 45 min ischemia, and the impact of ischemia on follicular survival and development status was evaluated at different time-points (1 day, 3 days, 1 week, 2 weeks and 1 month). Assessment of follicular quantity and morphology was carried out via histologic analysis. Follicle proliferating status was evidenced by immunostaining with proliferating cell nuclear antigen (PCNA), and the Hedgehog signaling pathway (Patched and Gli); was verified via TUNEL assay. Quantitative PCR was carried out to quantify the mRNA of target genes including PCNA, Patched, Gli, Caspase 3, Bax, and Bcl-2. Compared with its contralateral fresh controls, the morphology, proliferation and apoptosis of the follicles in the grafts showed no significant differences and most primordial follicles were quiescent. However, morphology and proliferation status were significantly decreased 1 week after grafting, in comparison with the longitudinal grafting time. Patched and Gli in the Hedgehog signaling pathway were activated in only the follicles of the grafts. Short-term ischemia slightly impacts follicular survival and development status in whole ovarian grafting. Receiving intervention in the first week post-transplantation might be helpful.
Obstetrical Department, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Shuangshuang
S
Xie
Surgical Laboratory, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Xing
X
Zhang
Centre for Reproductive Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Wenming
W
Chen
Centre for Reproductive Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Chichi
C
Xie
Transplantation Centre, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Wenwei
W
Chen
Oncological Surgery, the Second Affiliated Hospital of Zhejiang University School of Medcine, Hangzhou, Zhejiang, 310000, China.
Pu
P
Cheng
Centre for Reproductive Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Ying
Y
Zhou
Transplantation Centre, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Bicheng
B
Chen
D000818:Animals; D002452:Cell Count; D048430:Cell Shape; D002470:Cell Survival; D005260:Female; D006085:Graft Survival; D007511:Ischemia / Q000378:metabolism / Q000473:pathology*; D006080:Ovarian Follicle / Q000098:blood supply* / Q000473:pathology / Q000502:physiology*; D010053:Ovary / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D014183:Transplantation, Heterologous
D018809:Proliferating Cell Nuclear Antigen