Cloning and expression of mink (Neovison vison) interferon-γ gene and development of an antiviral assay.
Minks (Neovison vison) farming is under a threat of a variety of viral infections with increasingly growing number of breeding in Northeastern and Western China. While interferon is effective in controlling viral infection, IFN among different species rarely share high homology enough to provide cross protective effect on inhibition of virus replication. We cloned, sequenced, phlogenetically analyzed and expressed the miIFN-γ gene in prokaryotic and eukaryotic cells. The anti-vesicular stomatitis virus (VSV) activity of miIFN-γ protein was tested in MDCK cells using in vitro cytopathic inhibition assay. The recombinant miIFN-γ could inhibit VSV replication in MDCK cells, which was confirmed by that pre-incubation of rabbit anti-miIFN-γ antibodies with miIFN-γ abrogated the miIFN-γ protective effect. Our findings implicated that the miIFN-γ gene may be a potential counter measure against viral infection in the mink farming.
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China; State Key Laboratory for Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: zhanghailing@caas.cn.
Hailing
H
Zhang
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China. Electronic address: zhaojianjun@caas.cn.
Jianjun
J
Zhao
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China. Electronic address: xuebai_1982@126.com.
Xue
X
Bai
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China. Electronic address: zhanglei19790401@126.com.
Lei
L
Zhang
Jilin Agricultural University, China. Electronic address: Fansining@126.com.
Sining
S
Fan
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China. Electronic address: 0434-3379293@163.com.
Bo
B
Hu
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China. Electronic address: liuhaowinner@126.com.
Hao
H
Liu
Jilin Teyan Biotechnological Co. Ltd, 388 Liuying West Road, Changchun 130122, China. Electronic address: sare135790@163.com.
Dongliang
D
Zhang
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China. Electronic address: 33491935@qq.com.
Shujuan
S
Xu
Division of Infectious Diseases of Special Economic Animal, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun 130112, China; State Key Laboratory for Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: tcsyxj@126.com.
Xijun
X
Yan
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D004285:Dogs; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D008907:Mink / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D054243:Vesicular Stomatitis / Q000517:prevention & control*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D007371:Interferon-gamma
Antiviral activity; Interferon-γ; Mink