Vizardous: interactive analysis of microbial populations with single cell resolution.
BACKGROUND
Single cell time-lapse microscopy is a powerful method for investigating heterogeneous cell behavior. Advances in microfluidic lab-on-a-chip technologies and live-cell imaging render the parallel observation of the development of individual cells in hundreds of populations possible. While image analysis tools are available for cell detection and tracking, biologists are still confronted with the challenge of exploring and evaluating this data.


RESULTS
We present the software tool Vizardous that assists scientists with explorative analysis and interpretation tasks of single cell data in an interactive, configurable and visual way. With Vizardous, lineage tree drawings can be augmented with various, time-resolved cellular characteristics. Associated statistical moments bridge the gap between single cell and the population-average level.


METHODS
The software, including documentation and examples, is available as executable Java archive as well as in source form at https://github.com/modsim/vizardous.


BACKGROUND
k.noeh@fz-juelich.de.


BACKGROUND
Supplementary data are available at Bioinformatics online.
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Stefan
S
Helfrich
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Charaf E
CE
Azzouzi
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Christopher
C
Probst
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Johannes
J
Seiffarth
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Alexander
A
Grünberger
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Wolfgang
W
Wiechert
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Dietrich
D
Kohlheyer
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Germany.
Katharina
K
Nöh
D048230:Corynebacterium glutamicum / Q000502:physiology; D008856:Microscopy, Fluorescence; D059010:Single-Cell Analysis; D012984:Software; D059008:Time-Lapse Imaging / Q000379:methods*