A Novel 6'-N-Aminoglycoside Acetyltransferase, AAC(6')-Ial, from a Clinical Isolate of Serratia marcescens.
Serratia marcescens IOMTU115 has a novel 6'-N-aminoglycoside acetyltransferase-encoding gene, aac(6')-Ial. The encoded protein AAC(6')-Ial has 146 amino acids, with 91.8% identity to the amino acid sequence of AAC(6')-Ic in S. marcescens SM16 and 97.3% identity to the amino acid sequence of AAC(6')-Iap in S. marcescens WW4. The minimum inhibitory concentrations of aminoglycosides for Escherichia coli expressing AAC(6')-Ial were similar to those for E. coli expressing AAC(6')-Ic or AAC(6')-Iap. Thin-layer chromatography showed that AAC(6')-Ial, AAC(6')-Ic, or AAC(6')-Iap acetylated all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin. Kinetics assays revealed that AAC(6')-Ial is a functional acetyltransferase against aminoglycosides. The aac(6')-Ial gene was located on chromosomal DNA.
1 Department of Infectious Diseases, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
Tatsuya
T
Tada
2 Pathogenic Microbe Laboratory, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
Tohru
T
Miyoshi-Akiyama
1 Department of Infectious Diseases, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
Kayo
K
Shimada
3 Department of Microbiology, Institute of Medicine, Tribhuvan University , Kathmandu, Nepal .
Rajan K
RK
Dahal
3 Department of Microbiology, Institute of Medicine, Tribhuvan University , Kathmandu, Nepal .
Shyam K
SK
Mishra
4 Department of International Medical-Cooperation, National Center for Global Health and Medicine , Tokyo, Japan .
Hiroshi
H
Ohara
1 Department of Infectious Diseases, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
Teruo
T
Kirikae
3 Department of Microbiology, Institute of Medicine, Tribhuvan University , Kathmandu, Nepal .
Bharat M
BM
Pokhrel
D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000378:metabolism / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001711:Biotransformation; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008826:Microbial Sensitivity Tests; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D016868:Serratia Infections / Q000382:microbiology; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase