Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats.
BACKGROUND
This study was designed to evaluate the protective effect of Echium amoenum total anthocyanin extract (ETAE) on partial/transient cerebral ischemia in the rats.


METHODS
Rats received ETAE (50, 100 and 200 mg/kg, i.p.) 30 min before the induction of cerebral ischemia. Cerebral ischemia was induced by bilateral common carotid arteries occlusion (BCCAO) for 30 min, followed by 72 h reperfusion. The neurological deficit, brain performance, and sensory motor function were assessed 48 h and 72 h after surgery. After sacrification, the brains were evaluated for myeloperoxidase (MPO) activity and histopathological changes.


RESULTS
Our results showed that motor function significantly decreased in ischemia/reperfusion (I/R) group as compared to the sham group. Histopathological analysis exhibited the shrinkage and atrophy of the neurons in I/R group. ETAE at the dose of 200 mg/kg improved spontaneous activity and memory induced by cerebral ischemia compared to the control group and also decreased brain MPO activity following cerebral ischemia. However, it could not affect the ability to climbing, body proprioception, vibrissae touch and brain water content. In addition, pretreatment with ETAE at higher doses significantly reduced ischemia-induced neuronal loss of the brain.


CONCLUSIONS
The anthocyanin rich fraction from E. amoenum was found to have protective effects against some brain damages postischemic reperfusion. However, further researches are required for investigating the exact mechanisms of the effect of this plant in the prevention of cerebral ischemia in human.
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan Pharmaceutical Sciences Research Center, Isfahan, Iran.
Leila
L
Safaeian
Department of Physiology, Anatomical Research Center, Kashan University of Medical Science, Kashan, Iran.
Abolfazl Azami
AA
Tameh
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Alireza
A
Ghannadi
Department of Physiology, Physiology Research Center, Kashan University of Medical Science, Kashan, Iran.
Elmira Akbari
EA
Naghani
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan Pharmaceutical Sciences Research Center, Isfahan, Iran.
Hamed
H
Tavazoei
Department of Physiology, Physiology Research Center, Kashan University of Medical Science, Kashan, Iran.
Samaneh Sadat
SS
Alavi
Anthocyanins; Echium amoenum; cerebral ischemia; rat