Increase ofClostridium perfringensin association withEimeriain haemorrhagic enteritis in Japanese beef cattle.
A coprological survey with detailed clinical observation of naturally occurring haemorrhagic enteritis (HE) cases was conducted to understand the pathophysiology of HE by clarifying the infection status ofEimeriaand enteropathogenic bacteria in cattle. Faecal samples from 55 cases of HE and 26 clinically normal animals were collected, and a quantitative examination ofEimeriaand potential enteropathogenic bacteria was performed. The number ofEimeriaspecies oocysts per gram of faeces (OPG) exceeded 10,000 in 69.1 per cent of HE cases with a maximum of 1,452,500 OPG andEimeria zuerniiwas found to be overwhelmingly dominant. A significant increase in faecal coliform count was observed in HE cases compared with clinically normal animals. Among the animals shedding >10,000 OPG, 42.9 per cent showed a remarkable increase in Clostridium perfringensabundance (>104CFU/g) in the faeces. In the cases withC. perfringensdetected, its abundance was positively correlated withEimeriaOPG and highC. perfringensabundance was always accompanied by highEimeriaOPG.E. zuerniiis likely to play a crucial role in massive multiplication ofC. perfringensin HE in cattle.
Laboratory of Veterinary Parasitic Diseases, Interdisciplinary Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Y
Y
Kirino
Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
M
M
Tanida
Shepherd Central Livestock Clinic, Kagoshima, 20901 Akasegawa, Akune, Kagoshima 889-1611, Japan.
H
H
Hasunuma
Yamagata Prefectural Federation of Agricultural Mutual Relief Association, Central Vet. Clinic Center, 286-1 Kitakawahara, Nanaura, Yamagata 990-2171, Japan.
T
T
Kato
Laboratory of Veterinary Parasitic Diseases, Interdisciplinary Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
T
T
Irie
Laboratory of Veterinary Parasitic Diseases, Interdisciplinary Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.|Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.|Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Y
Y
Horii
Laboratory of Veterinary Parasitic Diseases, Interdisciplinary Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.|Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.|Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
N
N
Nonaka
Bacteriology; Cattle; Coccidiosis; Diarrhoea; Enteritis; Vitamin deficiency