[Expression of microRNA-10a-5p in laryngeal epithelial premalignant lesions].
OBJECTIVE
To investigate the expression of microRNA-10a-5p (miR-10a) in laryngeal epithelial premalignant lesions (LEPL) and to analyze the correlations of its dysregulation with clinicopathologic parameters of LEPL.


METHODS
According to the WHO classification (2005), 94 cases of LEPL were grouped into mild dysplasia (MID), moderate dysplasia (MOD), severe dysplasia (SD) and carcinoma in situ ( CIS). The expression of miR-10a in 94 cases of LEPL was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and correlated with the clinical and follow-up data of all LEPL patients.


RESULTS
miR-10a was down-regulated in LEPL with increasing grade of dysplasia. There was significantly statistical difference between low-risk ( MID + MOD) and high-risk ( SD + CIS) lesion groups (P = 0.03). The linear regression analysis showed that miR-10a was correlated with grade and gender of LEPL (P < 0.05).


CONCLUSIONS
Down regulation of miR-10a may be a useful molecular marker for grading of LEPL and diagnosis of early laryngeal cancer.
Yanping
Y
Hu
Yulan
Y
Jin
Xue
X
Li
Jia
J
Yu
Fang
F
Wang
Wenjing
W
Bu
Honggang
H
Liu
D015415:Biomarkers; D002278:Carcinoma in Situ / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D007830:Larynx / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology; D012334:RNA, Neoplasm / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
D015415:Biomarkers; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm