Sequence-specific 1H, 15N, and 13C resonance assignments of the autophagy-related protein LC3C.
Autophagy is a versatile catabolic pathway for lysosomal degradation of cytoplasmic material. While the phenomenological and molecular characteristics of autophagic non-selective (bulk) decomposition have been investigated for decades, the focus of interest is increasingly shifting towards the selective mechanisms of autophagy. Both, selective as well as bulk autophagy critically depend on ubiquitin-like modifiers belonging to the Atg8 (autophagy-related 8) protein family. During evolution, Atg8 has diversified into eight different human genes. While all human homologues participate in the formation of autophagosomal membrane compartments, microtubule-associated protein light chain 3C (LC3C) additionally plays a unique role in selective autophagic clearance of intracellular pathogens (xenophagy), which relies on specific protein-protein recognition events mediated by conserved motifs. The sequence-specific (1)H, (15)N, and (13)C resonance assignments presented here form the stepping stone to investigate the high-resolution structure and dynamics of LC3C and to delineate LC3C's complex network of molecular interactions with the autophagic machinery by NMR spectroscopy.
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany.|Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany.
Carsten
C
Krichel
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany.
Oliver H
OH
Weiergräber
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany.
Marina
M
Pavlidou
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany.
Jeannine
J
Mohrlüder
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany.
Melanie
M
Schwarten
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany.|Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany.
Dieter
D
Willbold
Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany. p.neudecker@fz-juelich.de.|Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany. p.neudecker@fz-juelich.de.
Philipp
P
Neudecker
D000595:Amino Acid Sequence; D001343:Autophagy; D002247:Carbon Isotopes; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D014316:Tritium
D002247:Carbon Isotopes; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D009587:Nitrogen Isotopes; D014316:Tritium
Autophagy; Heteronuclear NMR; LC3C; MAP1LC3C; Microtubule-associated protein light chain 3C; Sequence-specific resonance assignments