Monitoring TriAcylGlycerols Accumulation by Atomic Force Microscopy Based Infrared Spectroscopy in Streptomyces Species for Biodiesel Applications.
An atomic force microscope coupled with a tunable infrared laser source (AFM-IR) was used to measure the size and map the distribution of oil inclusions inside of microorganism without staining or other special sample preparation. The microorganism under study is Streptomyces, a soil bacterium that possesses the capability, under some specific nutritional conditions, to store its carbon source into TriAcylGlycerols, a potential direct source of biodiesel.
†Laboratoire de Chimie-Physique, Université Paris-Sud-CNRS UMR 8000, Orsay, France.
Ariane
A
Deniset-Besseau
‡Anasys Instruments, 325 Chapel Street, Suite 100, Santa Barbara, California 93101, United States.
Craig B
CB
Prater
§Institut de Génétique et Microbiologie, Groupe "Métabolisme Energétique des Streptomyces", Université Paris-Sud-CNRS UMR 8621, Orsay, France.
Marie-Joëlle
MJ
Virolle
†Laboratoire de Chimie-Physique, Université Paris-Sud-CNRS UMR 8000, Orsay, France.
Alexandre
A
Dazzi
infrared nanospectroscopy; soil filamentous bacteria; third-generation bio-oil