Preparation of photoreactive phospholipid polymer nanoparticles to immobilize and release protein by photoirradiation.
Photoreactive and cytocompatible polymer nanoparticles for immobilizing and releasing proteins were prepared. A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-4-(4-(1-methacryloyloxyethyl)-2-methoxy-5-nitrophenoxy) butyric acid (PL)) (PMB-PL) was synthesized. The PMB-PL underwent a cleavage reaction at the PL unit with photoirradiation at a wavelength of 365 nm. Additionally, the PMB-PL took polymer aggregate in aqueous medium and was used to modify the surface of biodegradable poly(L-lactic acid) (PLA) nanoparticle as an emulsifier. The morphology of the PMB-PL/PLA nanoparticle was spherical and approximately 130 nm in diameter. The carboxylic acid group in the PL unit could immobilize proteins by covalent bonding. The bound proteins were released by a photoinduced cleavage reaction. Within 60s, up to 90% of the immobilized proteins was released by photoirradiation. From these results and with an understanding of the fundamental properties of MPC polymers, we concluded that PMB-PL/PLA nanoparticles have the potential to be used as smart carriers to deliver proteins to biological systems, such as the inside of living cells.
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8586, Japan.
Weixin
W
Chen
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8586, Japan.
Yuuki
Y
Inoue
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8586, Japan; Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8586, Japan. Electronic address: ishihara@mpc.t.u-tokyo.ac.jp.
Kazuhiko
K
Ishihara
D004337:Drug Carriers; D055767:Immobilized Proteins / Q000737:chemistry / Q000528:radiation effects; D019344:Lactic Acid; D008027:Light; D008689:Methacrylates; D053758:Nanoparticles / Q000737:chemistry*; D010743:Phospholipids / Q000138:chemical synthesis*; D010767:Phosphorylcholine / Q000031:analogs & derivatives; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011506:Proteins / Q000737:chemistry* / Q000187:drug effects*
D004337:Drug Carriers; D055767:Immobilized Proteins; D008689:Methacrylates; D010743:Phospholipids; D011108:Polymers; D011506:Proteins; D010767:Phosphorylcholine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C070638:2-methacryloyloxyethyl phosphorylcholine; C016284:butyl methacrylate
Phospholipid polymer; Photoinduced releasing; Photoreactive polymer nanoparticle; Protein immobilization