Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts.
Adult skin stem cells are recognized as potential therapeutics to rejuvenate aged skin. We previously demonstrated that human dermal stem/progenitor cells (hDSPCs) with multipotent capacity could be enriched from human dermal fibroblasts using collagen type IV. However, the effects of hDSPCs on cellular senescence remain to be elucidated. In the present study, we investigated whether conditioned medium (CM) collected from hDSPC cultures (hDSPC-CM) exhibits beneficial effects on senescent fibroblasts. We found that hDSPC-CM promoted proliferation and decreased the expression level of senescence-associated β-galactosidase in senescent fibroblasts. In addition, p53 phosphorylation and p21 expression were significantly reduced in senescent fibroblasts treated with hDSPC-CM. hDSPC-CM restored the expression levels of collagen type I, collagen type III, and tissue inhibitor of metalloproteinase, and antagonized the increase of matrix metalloproteinase 1 expression. Finally, we demonstrated that hDSPC-CM significantly reduced reactive oxygen species levels by specifically up-regulating the expression level of superoxide dismutase 2. Taken together, these data suggest that hDSPC-CM can be applied as a potential therapeutic agent for improving human aged skin.
Amorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Geyonggi-do 446729, Korea. ninefog@amorepacific.com.
Ji-Yong
JY
Jung
Amorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Geyonggi-do 446729, Korea. ungstrong@hanmail.net.
Joong Hyun
JH
Shim
Amorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Geyonggi-do 446729, Korea. happycell@amorepacific.com.
Hyun
H
Choi
Amorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Geyonggi-do 446729, Korea. TRLee@amorepacific.com.
Tae Ryong
TR
Lee
Amorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Geyonggi-do 446729, Korea. biopang@empal.com.
Dong Wook
DW
Shin
D000328:Adult; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000302:isolation & purification / Q000378:metabolism*; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D000099156:Superoxide Dismutase 2
D017077:Culture Media, Conditioned; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2
fibroblast; human dermal stem/progenitor cells; senescence