Effect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injury.
OBJECTIVE
To study the effect of remote ischemic preconditioning (RIPC) in ischemia-reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model.


METHODS
Thirty-six male rats were divided in three groups: Sham; I/R injury, a 45 minutes lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of reperfusion for histopathological study, plasma cytokines and alanine aminotransferase (ALT) measurement.


RESULTS
The histopathological study demonstrated a significant reduction in liver necrosis in the RIPC group (p<0,001). The ALT levels were also significant lower in the RIPC group (p<0.01). The cytokines assessment showed that IL-6 levels were increased in the RIPC group after 1h of reperfusion, in comparison to the I/R group (p<0.05). Interleukin-10 levels in RIPC groups did not differ significantly from I/R group.


CONCLUSIONS
Remote ischemic preconditioning is effective in decreasing liver necrosis in a rat model of ischemia-reperfusion. The IL-6 expression is up-regulated and peaked at 60 min of reperfusion. There was no difference in IL-10 expression between the groups.
Department of General Surgery, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil.
Marco Antonio Correa
MA
Guimarães Filho
Department of Histology and Embryology, UERJ, Rio de Janeiro, RJ, Brazil.
Erika
E
Cortez
Department of Physiological Sciences, UERJ, Rio de Janeiro, RJ, Brazil.
Érica Patrícia
ÉP
Garcia-Souza
UERJ, Rio de Janeiro, RJ, Brazil.
Vivian de Melo
Vde M
Soares
Department of Physiological Sciences, UERJ, Rio de Janeiro, RJ, Brazil.
Aníbal Sanchez
AS
Moura
Department of Histology and Embryology, UERJ, Rio de Janeiro, RJ, Brazil.
Laís
L
Carvalho
Department of General Surgery, UERJ, Rio de Janeiro, RJ, Brazil.
Maria Cristina de Araujo
MC
Maya
Department of General Surgery, UERJ, Rio de Janeiro, RJ, Brazil.
Marcos Bettini
MB
Pitombo
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D019194:Ischemic Preconditioning / Q000379:methods*; D008099:Liver / Q000098:blood supply* / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000473:pathology / Q000517:prevention & control; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*; D015203:Reproducibility of Results; D013997:Time Factors
D015850:Interleukin-6; D016753:Interleukin-10; D000410:Alanine Transaminase