Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats.
Glucagon-like peptide-2 (GLP-2) has potent anti-inflammatory effects and protects against experimental ischemia/reperfusion (I/R) injury in pulmonary, intestinal, and myocardial tissue. However, its protective abilities against I/R injury in the liver are unknown. We investigated the potential role of GLP-2 pretreatment on hepatic I/R injury in rats. A total of 24 rats were randomly divided into three groups (n = 8). The first group was the control group; the second group was the vehicle-treated hepatic ischemia/reperfusion (HIR, vehicle saline-treated) group; and the third group was the GLP-2 pretreated I/R (GLP2-IR) group. Each rat in the third group was intraperitoneally administered 5 µg GLP-2 for 5 d before the procedure. A portal triad was created to induce ischemia with a vascular atraumatic clamp. After 40 min, the clamp was released to initiate hepatic reperfusion for 6 h. Blood samples and tissue specimens from the liver were obtained. Alanine aminotransferase, aspartate aminotransferase, and total bilirubin levels significantly increased in the salinetreated HIR group (P < 0.001), whereas GLP-2 pretreatment significantly decreased their levels (P < 0.01). Our data suggested that GLP-2 pretreatment may have a protective effect on liver I/R injury. However, dose-response studies are necessary to determine the most effective dose.
Medical Faculty, Department of Pediatrics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
Naci
N
Topaloğlu
Adem
A
Küçük
Şule
Ş
Yıldırım
Mustafa
M
Tekin
Havva
H
Erdem
Mustafa
M
Deniz
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood*; D004195:Disease Models, Animal; D053767:Glucagon-Like Peptide 2 / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*
D053767:Glucagon-Like Peptide 2; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin