Elevated expression of periostin in diabetic cardiomyopathy and the effect of valsartan.
BACKGROUND
Periostin, an extracellular matrix protein, plays a significant role in adverse cardiac remodeling. However, no report has documented the function of periostin in left ventricular remodeling of streptozototin (STZ)-induced diabetic rats. The aim of the present study was to observe the expression of periostin in Wistar rat's myocardium of diabetic cardiomyopathy (DCM) and the effect of valsartan on it.


METHODS
Immunohistochemistry, real-time polymerase chain reaction, and Western blot analysis were used to determine the degree of expression and location of periostin, transforming growth factor (TGF)-β1, TGF-β1 type II receptor (TGF-β1 R II), and Type I and III collagens in the myocardium of STZ-induced diabetic rats.


RESULTS
Periostin, TGF-β1, TGF-β1 R II, and Type I and III collagens were significantly increased in the myocardium of diabetic rats compared with control group on both messenger ribonucleic acid and protein levels. In addition, diabetic rats treated with valsartan could have reduced expression of periostin and improved cardiac remodeling of DCM.


CONCLUSIONS
Periostin may play a crucial role in cardiac remodeling and myocardial interstitial fibrosis process of DCM and it could be one of the important mechanisms for valsartan to improve the ventricular remodeling of DCM.
Department of Cardiology, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Jun
J
Guan
Department of Cardiology, Qingdao Municipal Hospital, Qingdao, Shandong, China.|Qingdao University Medical College, Qingdao, Shandong, China.|Key Laboratory of cellular transplantation , Chinese Ministry of Public Health, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Wen-Qi
WQ
Liu
Department of Clinical laboratory, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Ming-Qing
MQ
Xing
Qingdao University Medical College, Qingdao, Shandong, China.
Yue
Y
Shi
Qingdao University Medical College, Qingdao, Shandong, China.|Key Laboratory of cellular transplantation , Chinese Ministry of Public Health, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Xue-Ying
XY
Tan
Department of pathology department, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Chang-Qing
CQ
Jiang
Department of Cardiology, Qingdao Municipal Hospital, Qingdao, Shandong, China. daihy9@163.com.
Hong-Yan
HY
Dai
D047228:Angiotensin II Type 1 Receptor Blockers / Q000627:therapeutic use; D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism*; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy* / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D000068756:Valsartan / Q000627:therapeutic use*
D047228:Angiotensin II Type 1 Receptor Blockers; D015815:Cell Adhesion Molecules; C512836:Postn protein, rat; D000068756:Valsartan