l-amino acid oxidase from Bothrops marajoensis causes nephrotoxicity in isolated perfused kidney and cytotoxicity in MDCK renal cells.
Renal alterations caused by Bothrops venom and its compounds are studied to understand these effects and provide the best treatment. Previously, we studied the renal effect of the whole venom of Bothrops marajoensis and its phospholipase A2 (PLA2), but these effects could not to be attributed to PLA2. To continue the study, we report in this short communication the effects of l-amino acid oxidase from B. marajoensis venom (LAAOBm) on renal function parameter alterations observed in the same model of isolated perfused kidney, as well as the cytotoxic effect on renal cells. LAAOBm caused a decrease in PP, RVR, UF, GFR, %TNa(+) and %TCl(-), very similar to the effects of whole venom using the same model. We also demonstrated its cytotoxicity in MDCK cells with IC50 of 2.5 μg/mL and late apoptotic involvement demonstrated by flow cytometry assays. In conclusion, we suggested that LAAOBm is a nephrotoxic compound of B. marajoensis venom.
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Rodrigo Tavares
RT
Dantas
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Antônio Rafael Coelho
AR
Jorge
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Roberta Jeane Bezerra
RJ
Jorge
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Ramon Róseo Paula Pessoa Bezerra de
RR
Menezes
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza 60430370, Ceara, Brazil.
Danya Bandeira
DB
Lima
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza 60430370, Ceara, Brazil.
Alba Fabíola Costa
AF
Torres
Sao Vicente Unit, Paulista Coastal Campus, Sao Paulo State University (UNESP), Sao Paulo, Brazil.
Marcos Hikari
MH
Toyama
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Helena Serra Azul
HS
Monteiro
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza 60430370, Ceara, Brazil. Electronic address: martinsalice@gmail.com.
Alice Maria Costa
AM
Martins
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D017837:Bothrops; D002470:Cell Survival / Q000187:drug effects; D003435:Crotalid Venoms / Q000633:toxicity*; D004285:Dogs; D004847:Epithelial Cells / Q000187:drug effects; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000187:drug effects*; D007674:Kidney Diseases / Q000209:etiology / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects; D050830:L-Amino Acid Oxidase / Q000633:toxicity*; D061985:Madin Darby Canine Kidney Cells; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D003435:Crotalid Venoms; D050830:L-Amino Acid Oxidase
Bothrops marajoensis venom; LAAO; Nephrotoxicity