Suppressive Effect of Dietary Fucoidan on Proinflammatory Immune Response and MMP-1 Expression in UVB-Irradiated Mouse Skin.
It is well known that ultraviolet B irradiation leads to dermal inflammation. In this study, we found that Mekabu fucoidan suppressed edema, decreased the thickness of the prickle cell layer, and decreased matrix metalloproteinase 1 in the skin of mice irradiated with ultraviolet B. Moreover, we found that the mean level of interferon gamma of Mekabu fucoidan-treated, ultraviolet B-irradiated mice (approximately 2.2 ng/mL) was not significantly different from that in normal mice (approximately 2.5 ng/mL). In contrast, a significant decrease in the mean level of interferon gamma (approximately 1.3 ng/mL) in ultraviolet B-irradiated control mice was observed compared with that in Mekabu fucoidan-treated, ultraviolet B-irradiated mice. The mean thickness of the prickle cell layer in the skin of Mekabu fucoidan-treated, ultraviolet B-irradiated mice was less than that in the ultraviolet B-irradiated control mice. Metalloproteinase 1 activity was significantly higher in the skin of ultraviolet B-irradiated mice than in the skin of untreated, nonirradiated normal mice. Metalloproteinase 1 in the skin of ultraviolet B-irradiated, Mekabu fucoidan- or L(+)-ascorbic acid (vitamin C)-treated mice was significantly lower than that in the ultraviolet B-irradiated control mice. Mitigation of the morphological changes in Mekabu fucoidan-treated mice was correlated with a decrease in metalloproteinase 1 levels. These data indicate that Mekabu fucoidan is an effective suppressor of inflammation in an ultraviolet B-irradiated mouse model.
Department of Cytopathology, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Japan.
Hiroko
H
Maruyama
Department of Biochemistry, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Japan.
Hidekazu
H
Tamauchi
Department of Immunology, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Japan.
Fumitaka
F
Kawakami
Riken Vitamin Co., Ltd., Tokyo, Japan.
Keiko
K
Yoshinaga
Riken Vitamin Co., Ltd., Tokyo, Japan.
Takahisa
T
Nakano
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D003872:Dermatitis / Q000517:prevention & control*; D004032:Diet; D007109:Immunity / Q000187:drug effects; D007165:Immunosuppression Therapy; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis*; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays; D044648:Undaria / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011134:Polysaccharides; C007789:fucoidan; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse; D001205:Ascorbic Acid