Response of oxidative stress and inflammatory biomarkers to a 12-week aerobic exercise training in women with metabolic syndrome.
Evidences have been highlighted the relationship among metabolic syndrome, chronic low-grade inflammation, oxidative stress and several diseases. In this sense, the aim of this study was to investigate the effects of aerobic exercise training on oxidative stress and inflammatory parameters on women with metabolic syndrome (MS).



Twenty-three untrained women (51.86 ± 6.58 years old, BMI 30.8 ± 4.3 kg/m2) completed a 12-week treadmill exercise training, without modifications on dietary pattern. Advanced oxidation protein products (AOPP), thiobarbituric acid-reactive substances (TBARS), total thiol content (T-SH) and nitrite and nitrate (NOx) levels were assessed in plasma while the levels of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-α) and interferon-gamma (IFN-γ) were evaluated in the serum. The RNA expression (mRNA) of IL-1β, IL-10, TNF-α, IFN-γ, insulin receptor substrate 2 (IRS-2) and matrix metalloproteinase-9 (MMP-9) were performed inperipheral blood mononuclear cells (PBMC) of a subset with eight women with MS using real real-time polymerase chain reaction (qPCR).



The intervention resulted in decreased serum levels of IL-1β, IL-6, TNF-α, IFN-γ, AOPP and TBARS, besides increased levels of IL-10 and T-SH (P< 0.001). NOx concentrations were unchanged, similarly to mRNA expressions quantified in PBMC.



Twelve weeks of AT improved systemic oxidative stress and inflammatory biomarkers in women with MS, although PBMC mRNA expression for inflammatory pathways appeared to be unchanged. This may indicate that AT induced beneficial effects not only in physical fitness but also on health promotion through decreased oxidative damage and proinflammatory status.
Departamento de Métodos e Técnicas Desportivas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil ; Escola Superior de Educação Física, Universidade Federal do Rio Grande do Sul, Rua Felizardo 750, Porto Alegre, 90690200 Brazil.
Juliano Boufleur
JB
Farinha
Departamento de Métodos e Técnicas Desportivas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Flávia Mariel
FM
Steckling
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Sílvio Terra
ST
Stefanello
Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Manuela Sangoi
MS
Cardoso
Centro Universitário Franciscano, Rua dos Andradas 1614, Santa Maria, 97010-032 Brazil.
Larissa Santos
LS
Nunes
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Rômulo Pillon
RP
Barcelos
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Thiago
T
Duarte
Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, 90035-903 Brazil.
Nélson Alexandre
NA
Kretzmann
Departamento de Métodos e Técnicas Desportivas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Carlos Bolli
CB
Mota
Facultad de Ciencias de la Salud, Universidade Autónoma de Chile, Avenida Alemania 01090, Temuco, 4810101 Chile.
Guilherme
G
Bresciani
Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Rafael Noal
RN
Moresco
Universidade Luterana do Brasil, BR 287 km 252, Santa Maria, 97020-001 Brazil.
Marta Maria Medeiros Frescura
MM
Duarte
Departamento de Métodos e Técnicas Desportivas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Daniela Lopes
DL
Dos Santos
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, 97105-900 Brazil.
Félix Alexandre Antunes
FA
Soares