Mining featured micro ribonucleic acids associated with lung cancer based on bioinformatics.
BACKGROUND
Few genetic markers useful for the screening of lung cancer risk exist. Although related research has shown that certain expression profiles of micro ribonucleic acids (miRNAs) are different in lung cancer versus the normal lung, such as miR-29a and miR-29s, the precise molecular mechanism of lung cancer remains obscure. In order to get a better understanding of the pathogenetic mechanism of lung cancer, we analyzed the differentially expressed genes (DEGs) and identified featured miRNAs in lung cancer tissues.


METHODS
We used the gene expression profile GSE10072, including 49 gene chips of non-tumor tissues and 58 gene chips of lung tumor specimens. The DEGs between these two groups were identified by Limma package in R language. The TarBase database was used to construct the networks of miRNA regulating DEGs related to lung cancer. After ordering miRNAs regulating DEGs, we further screened featured miRNAs combined with the miR2Disease database.


RESULTS
A total of 5572 DEGs were obtained between lung cancer and control specimens. After constructing a miRNA regulatory network, a total of 398 regulations between 57 miRNAs and 321 target genes existed. By intergrating the miR2Disease database and using a sorting algorithm, a total of six featured miRNAs related to lung cancer were identified, including miR-520h, miR-133a, miR-34, miR-103, miR-370, and miR-148. They might be involved in lung cancer progression by regulating ABCG2, PKM2, VAMP2, GPD1, MAP3K8, and DNMT3B, respectively.


CONCLUSIONS
The top 10 significant miRNAs, such as miR-520h, miR-133a, miR-34, and miR-103 may be potential therapeutic targets for lung cancer.
Department of Respiratory, The Fourth People's Hospital of Jinan, Jinan Clinical School of Taishan Medical College Jinan, China.
Lin
L
Su
Department of Clinical Pharmacy, The Fourth People's Hospital of Jinan, Jinan Clinical School of Taishan Medical College Jinan, China.
Na
N
Li
Department of Respiratory, The Fourth People's Hospital of Jinan, Jinan Clinical School of Taishan Medical College Jinan, China.
Xueyun
X
Huo
DNA microarray; Differentially expressed genes; lung cancer; miRNA