eIF4AII is dispensable for miRNA-mediated gene silencing.
MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3' untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying mechanistic details of this process are not well understood. Recently, eukaryotic initiation factor (eIF) 4AII was identified as a critical modulator of miRNA activity with depletion of this factor alleviating miRNA-mediated gene repression. Using the CRISPR/Cas9-editing system, we generated a novel cell line in which expression of eIF4AII was eliminated. The absence of eIF4AII does not affect cell viability, proliferation, or global mRNA translation. Importantly, we show that eIF4AII is dispensable for miRNA-mediated gene silencing.
Department of Biochemistry, McGill University, Montreal, Québec H3G 1Y6, Canada.
Gabriela
G
Galicia-Vázquez
Department of Biochemistry, McGill University, Montreal, Québec H3G 1Y6, Canada.
Jennifer
J
Chu
Department of Biochemistry, McGill University, Montreal, Québec H3G 1Y6, Canada The Rosalind and Morris Goodman Cancer Research Center, McGill University, Montreal, Québec H3G 1Y6, Canada Department of Oncology, McGill University, Montreal, Québec H3G 1Y6, Canada.
Jerry
J
Pelletier
D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D039601:Eukaryotic Initiation Factor-4A / Q000502:physiology*; D020868:Gene Silencing / Q000502:physiology*; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D041681:NIH 3T3 Cells
D035683:MicroRNAs; D039601:Eukaryotic Initiation Factor-4A
CRISPR; Cas9; RISC; eIF4AII; genome editing; miRNA