Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans.
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced. In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790. Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival. Taken together, these results indicate that the DR1790 protein from the ancient yellow protein family plays a pleiotropic role in the survival of prokaryotic cells and contributes to the extraordinary resistance of D. radiodurans against oxidative and radiation stresses.
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China, Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Jianhui
J
Cheng
Institute of Ageing Research, Hangzhou Normal University, Hangzhou, China, Institute of Ageing Research, Hangzhou Normal University, Hangzhou, China.
Hu
H
Wang
Zhejiang University, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
Xin
X
Xu
Zhejiang University, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
Liangyan
L
Wang
Zhejiang University, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
Bing
B
Tian
Zhejiang University, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
Yuejin
Y
Hua
D001426:Bacterial Proteins / Q000235:genetics; D002462:Cell Membrane / Q000502:physiology; D034301:Deinococcus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D058685:Genetic Pleiotropy; D006861:Hydrogen Peroxide / Q000633:toxicity; D008565:Membrane Proteins / Q000235:genetics; D046228:Microarray Analysis; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D016254:Mutagenesis, Insertional; D010539:Permeability; D011839:Radiation, Ionizing; D060888:Real-Time Polymerase Chain Reaction
D001426:Bacterial Proteins; D008565:Membrane Proteins; D006861:Hydrogen Peroxide
Deinococcus radiodurans; characteristics; dr1790 disruptant; functional analysis