Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin.
With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore: nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37-3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap, and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.
Center for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, 52210 Rovinj, Croatia.
Filip
F
Stipić
Center for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, 52210 Rovinj, Croatia.
Petra
P
Burić
Center for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, 52210 Rovinj, Croatia.
Željko
Ž
Jakšić
Department of Marine and Environmental Research, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia.
Galja
G
Pletikapić
Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia.
Maja
M
Dutour Sikirić
Time Resolved X-Ray Spectroscopy Laboratory, Elettra-Sincrotrone Trieste, 34149 Basovizza, Italy.
Goran
G
Zgrablić
Department of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia.
Leo
L
Frkanec
Center for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, 52210 Rovinj, Croatia. Electronic address: lyons@irb.hr.
Daniel M
DM
Lyons
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D060807:Bioluminescence Resonance Energy Transfer Techniques; D015374:Biosensing Techniques; D049872:Bivalvia; D003102:Colloids / Q000737:chemistry*; D005456:Fluorescent Dyes; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008387:Marine Toxins / Q000032:analysis* / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles; D019319:Okadaic Acid / Q000032:analysis* / Q000276:immunology; D010316:Particle Size; D057096:Shellfish Poisoning / Q000175:diagnosis
D000906:Antibodies; D003102:Colloids; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D008387:Marine Toxins; D019319:Okadaic Acid
Biosensor; Diagnostic; Immunoassay; Lanthanide; Nanoparticle; Point-of-care