Oral Streptococci Biofilm Formation on Different Implant Surface Topographies.
The establishment of the subgingival microbiota is dependent on successive colonization of the implant surface by bacterial species. Different implant surface topographies could influence the bacterial adsorption and therefore jeopardize the implant survival. This study evaluated the biofilm formation capacity of five oral streptococci species on two titanium surface topographies. In vitro biofilm formation was induced on 30 titanium discs divided in two groups: sandblasted acid-etched (SAE- n = 15) and as-machined (M- n = 15) surface. The specimens were immersed in sterilized whole human unstimulated saliva and then in fresh bacterial culture with five oral streptococci species: Streptococcus sanguinis, Streptococcus salivarius, Streptococcus mutans, Streptococcus sobrinus, and Streptococcus cricetus. The specimens were fixed and stained and the adsorbed dye was measured. Surface characterization was performed by atomic force and scanning electron microscopy. Surface and microbiologic data were analyzed by Student's t-test and two-way ANOVA, respectively (P < 0.05). S. cricetus, S. mutans, and S. sobrinus exhibited higher biofilm formation and no differences were observed between surfaces analyzed within each species (P > 0.05). S. sanguinis exhibited similar behavior to form biofilm on both implant surface topographies, while S. salivarius showed the lowest ability to form biofilm. It was concluded that biofilm formation on titanium surfaces depends on surface topography and species involved.
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Pedro Paulo Cardoso
PP
Pita
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil ; Department of Operative Dentistry, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
José Augusto
JA
Rodrigues
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Claudia
C
Ota-Tsuzuki
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Tatiane Ferreira
TF
Miato
Department of Oral Implantology, PUC Minas, Avenida Dom Cabral 500, Prédio 46, Coração Eucarístico, 30535-901 Belo Horizonte, MG, Brazil.
Elton G
EG
Zenobio
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Gabriela
G
Giro
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Luciene C
LC
Figueiredo
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Cristiane
C
Gonçalves
Biotecnos-Tecnologia e Ciência Ltda, Rua Dr. Bozano, 571 Centro, 97015-001 Santa Maria, RS, Brazil.
Sergio A
SA
Gehrke
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Alessandra
A
Cassoni
Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Praça Tereza Cristina 229, 07023-070 Guarulhos, SP, Brazil.
Jamil Awad
JA
Shibli
D018441:Biofilms / Q000254:growth & development*; D015921:Dental Implants / Q000382:microbiology*; D003764:Dental Materials / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D012463:Saliva / Q000382:microbiology*; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification* / Q000254:growth & development*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D015921:Dental Implants; D003764:Dental Materials; D014025:Titanium