Evaluation of skeletal muscle relaxant activity of aqueous extract of Nerium oleander flowers in Albino rats.
OBJECTIVE
Nerium oleander is traditionally used in various diseases because of its medicinal properties. One of its uses is in musculoskeletal disorder. The aim of the study was to evaluate the skeletal muscle relaxant activity of the aqueous extract of Nerium oleander flowers (AENOF) in albino rats in comparison with diazepam.


METHODS
A total of 20 Swiss albino rats aged 6-7 weeks, of either sex, weighing about 100-150 g, were taken, and after acute toxicity studies two different doses were selected. The animals were divided into four different groups. The first group was kept as the control (normal saline), second as the standard (diazepam) and the remaining two groups as Test I and Test II, and given different doses of the AENOF. Skeletal muscle relaxant activity (motor coordination) on Rotarod and locomotor activity on photoactometer was performed. Statistical analysis was carried out by using analysis of variance, followed by Dunnett's multiple comparison tests.


RESULTS
The result from the Actophotometer test and Rotarod test showed that the extract of AENOF significantly reduced (P < 0.05) the motor coordination of the tested animals.


CONCLUSIONS
Our data indicates that AENOF possesses skeletal muscle relaxant activities.
Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India.
Jayasree
J
Tirumalasetti
Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India.
Maulik
M
Patel
Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India.
Ubedulla
U
Shaikh
Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India.
K
K
Harini
Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India.
J
J
Shankar
D000818:Animals; D003975:Diazepam / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D035264:Flowers; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D030007:Nerium / Q000737:chemistry*; D009465:Neuromuscular Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D023382:Toxicity Tests, Acute
D009465:Neuromuscular Agents; D010936:Plant Extracts; D003975:Diazepam
Actophotometer; Nerium oleander; muscle relaxant; rotarod; soxhlet apparatus