Activity in mice of recombinant BCG-EgG1Y162 vaccine for Echinococcus granulosus infection.
Cystic hydatid disease is a zoonotic parasitic disease caused by Echinococcus granulosus which is distributed worldwide. The disease is difficult to treat with surgery removal is the only cure treatment. In the high endemic areas, vaccination of humans is believed a way to protect communities from the disease. In this study we vaccinated BALB/c mice with rBCG-EgG1Y162, and then detected the level of IgG and IgE specifically against the recombinant protein by ELISA, rBCG-EgG1Y162 induced strong and specific cellular and humoral immune responses. In vitro study showed that rBCG-EgG1Y162 vaccine not only promote splenocytes proliferation but also active T cell. In addition, the rBCG-EgG1Y162 induced a protection in the mice against secondary infection of Echinococcus granulosus.
a State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294); Xinjiang Key Laboratory of Echinococcosis; First Affiliated Hospital of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.|b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Xiumin
X
Ma
a State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294); Xinjiang Key Laboratory of Echinococcosis; First Affiliated Hospital of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Hui
H
Zhao
a State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294); Xinjiang Key Laboratory of Echinococcosis; First Affiliated Hospital of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Fengbo
F
Zhang
a State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294); Xinjiang Key Laboratory of Echinococcosis; First Affiliated Hospital of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Yuejie
Y
Zhu
b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Shanshan
S
Peng
b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Haimei
H
Ma
b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Chunbao
C
Cao
b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Yan
Y
Xin
b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Delixiati
D
Yimiti
a State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294); Xinjiang Key Laboratory of Echinococcosis; First Affiliated Hospital of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Hao
H
Wen
a State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294); Xinjiang Key Laboratory of Echinococcosis; First Affiliated Hospital of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.|b College of Basic Medicine of Xinjiang Medical University ; Urumqi , Xinjiang , PR China.
Jianbing
J
Ding
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000235:genetics; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004443:Echinococcosis / Q000517:prevention & control*; D048209:Echinococcus granulosus / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001500:BCG Vaccine; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D007073:Immunoglobulin E
Echinococcus granulosus; EgG1Y162; vaccine