Melatonin and selenium reduce plasma cytokine and brain oxidative stress levels in diabetic rats.
BACKGROUND
Hyperglycaemia-induced progression of brain and erythrocyte oxidative injuries might be modulated by melatonin and selenium as potent antioxidants. The present study was conducted to explore whether melatonin and selenium protect against diabetic brain and erythrocyte oxidative stress levels in streptozotocin (STZ)-induced diabetic rats.


METHODS
Seventy rats were equally divided into seven groups. The first and second groups were used as untreated and placebo treated controls. The third group was treated with STZ to induce diabetes. The fourth and sixth groups received 10 mg kg(-1) melatonin. The fifth and seventh groups were treated with 1.5 mg kg(-1) selenium (sodium selenite). The sixth and seventh groups were treated with STZ administered with melatonin and selenium as described for the fourth and fifth groups.


RESULTS
Brain and erythrocyte lipid peroxidation levels and plasma IL-1β and IL-4 levels were high in the STZ group, although they were low in melatonin and selenium treatments. Decreased glutathione peroxidase, reduced glutathione, total antioxidant status, vitamins A and vitamin E values in brain and erythrocyte of STZ group were increased by melatonin and selenium treatments.


CONCLUSIONS
Melatonin and selenium induced protective effects against diabetes-induced brain and erythrocyte oxidative injuries through regulation of the antioxidant level and cytokine production.
a Department of Biophysics, Faculty of Medical Faculty , Izmir Katip Çelebi University , İzmir , Turkey .
Mehmet Cemal
MC
Kahya
b Department of Biophysics, Faculty of Medical Faculty , Suleyman Demirel University , Isparta , Turkey , and.|c Department of Neuroscience, Institute of Health Science , Suleyman Demirel University , Isparta , Turkey.
Mustafa
M
Naziroğlu
c Department of Neuroscience, Institute of Health Science , Suleyman Demirel University , Isparta , Turkey.
Bilal
B
Çiğ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D003921:Diabetes Mellitus, Experimental; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006943:Hyperglycemia; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000494:pharmacology; D008550:Melatonin / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D016207:Cytokines; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D012643:Selenium; D008550:Melatonin
Brain oxidative injury; cytokine; diabetes; erythrocytes; melatonin; selenium