In vitro acaricidal properties of Semecarpus anacardium fruit and Datura stramonium leaf extracts against acaricide susceptible (IVRI-I line) and resistant (IVRI-V line) Rhipicephalus (Boophilus) microplus.
In an attempt to identify plants having anti-tick properties, the 95% ethanolic and 50% hydro-ethanolic extracts of the fruits of Semecarpus anacardium and leaves of Datura stramonium were evaluated against reference tick lines of Rhipicephalus (Boophilus) microplus. The 95% ethanolic extracts of S. anacardium and D. stramonium caused 50% and 20% mortality, respectively, within 72 h of treatment by adult immersion test. The LC90 value of the ethanolic fruit extract of S. anacardium was determined as 13.5% (CI 12.05-15.12). The extract was also found efficacious (73.3%±3.3%) against the multi-acaricide-resistant IVRI-V line of R.(B.) microplus. The S. anacardium extract significantly affected the reproductive physiology of treated ticks by inhibiting the oviposition and was found safe. The HPTLC fingerprinting profile revealed the presence of pyrocatechol as a marker compound. The acaricidal property of S. anacardium against chemical acaricide-resistant R. (B.) microplus was discussed.
Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, UP, India. Electronic address: sghoshtick@gmail.com.
Srikant
S
Ghosh
Division of Pharmacognosy & Ethnopharmacology, National Botanical Research Institute, Rana Pratap Marg, P.B. 436, Lucknow 226001, UP, India.
Shashi Shankar
SS
Tiwari
Division of Pharmacognosy & Ethnopharmacology, National Botanical Research Institute, Rana Pratap Marg, P.B. 436, Lucknow 226001, UP, India.
Sharad
S
Srivastava
Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, UP, India.
Sachin
S
Kumar
Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, UP, India.
Anil Kumar
AK
Sharma
Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, UP, India.
Gaurav
G
Nagar
Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, UP, India.
K G Ajith
KG
Kumar
Division of Agricultural Chemicals, Indian Agricultural Research Institute, Pusa, New Delhi 110012, India.
Rajesh
R
Kumar
Division of Pharmacognosy & Ethnopharmacology, National Botanical Research Institute, Rana Pratap Marg, P.B. 436, Lucknow 226001, UP, India.
A K S
AK
Rawat
D056810:Acaricides / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D013286:Datura stramonium / Q000737:chemistry*; D005260:Female; D005638:Fruit / Q000737:chemistry*; D007814:Larva / Q000187:drug effects; D010058:Oviposition / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D048494:Rhipicephalus / Q000187:drug effects*; D031023:Semecarpus / Q000737:chemistry*
D056810:Acaricides; D010936:Plant Extracts
Datura stramonium; Ethanolic and hydro-ethanolic extracts; Pyrocatechol; Resistant ticks; Rhipicephalus (Boophilus) microplus; Semecarpus anacardium