Separation and identification of bioactive compounds in Anabasis articulata (Forsk) Moq. roots.
In the present paper, for the first time, we are interested to separate and identify some bioactive fractions isolated from the roots of a Saharan plant Anabasis articulata, which is widely used in traditional medicine against cancer. The crude methanolic extract of the roots was fractionated on column chromatography, and eluted with dichloromethane/methanol each time with increasing polarity of methanol; 17 fractions were separated. One of these fractions named F12 showed more antioxidant activity to scavenge DPPH free radical with percentage inhibition of 95.29%. F12 was separated by thin-layer chromatography (TLC) to give 12 compounds. A second preparative TLC of compound 2, which has antioxidative activity of 74.92%, provided the three phenolic acids M1, M2 and M3, analysed by high-performance liquid chromatography and UV-visible spectrophotometry.
a Laboratoire des Produits Naturels (LAPRONA), Département de Biologie , Faculté des Science de la Nature et de la Vie, des Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid , BP 119, Imama, Tlemcen , Algérie.
Nacéra
N
Ghembaza
a Laboratoire des Produits Naturels (LAPRONA), Département de Biologie , Faculté des Science de la Nature et de la Vie, des Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid , BP 119, Imama, Tlemcen , Algérie.
Nabila
N
Belyagoubi-Benhammou
a Laboratoire des Produits Naturels (LAPRONA), Département de Biologie , Faculté des Science de la Nature et de la Vie, des Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid , BP 119, Imama, Tlemcen , Algérie.
Fawzia
F
Atik Bekkara
D027703:Amaranthaceae / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D064209:Phytochemicals / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts
Anabasis articulata; DPPH; HPLC; UV–visible spectrophotometry; antioxidant activity; phenolic compounds