A DEVH-box RNA Helicase from Leishmania braziliensis is Associated to mRNA Cytoplasmic Granules.
RNA helicases are ubiquitous enzymes that participate in almost all aspects of RNA processing, including RNA and RNA-protein complex remodelling. In trypanosomatids, which post-transcriptionally regulate gene expression, the formation of different kinds of ribonucleoprotein granules under stress conditions modulates the parasite's RNA metabolism. This paper describes the isolation of a putative DEVH-box RNA helicase produced by promastigotes of Leishmania braziliensis. Using a Cy3-labelled dT30 oligo, FISH showed the localization of this protein to mRNA granules under starvation stress conditions. The central region of the protein was shown to be responsible for this behaviour.
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofisico Fco. Sánchez s/n, 38207 Tenerife, Spain; Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina López Neyra-CSIC, Parque Tecnológico de Ciencias de la Salud, 18016 Granada, Spain.
Raquel N
RN
Afonso-Lehmann
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina López Neyra-CSIC, Parque Tecnológico de Ciencias de la Salud, 18016 Granada, Spain.
Maria C
MC
Thomas
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofisico Fco. Sánchez s/n, 38207 Tenerife, Spain.
Maria A
MA
Santana-Morales
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofisico Fco. Sánchez s/n, 38207 Tenerife, Spain.
Daniel
D
Déniz
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina López Neyra-CSIC, Parque Tecnológico de Ciencias de la Salud, 18016 Granada, Spain.
Manuel C
MC
López
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofisico Fco. Sánchez s/n, 38207 Tenerife, Spain.
Basilio
B
Valladares
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofisico Fco. Sánchez s/n, 38207 Tenerife, Spain. Electronic address: emartine@ull.es.
Enrique
E
Martínez-Carretero
D000595:Amino Acid Sequence; D001483:Base Sequence; D003594:Cytoplasmic Granules / Q000201:enzymology*; D007892:Leishmania braziliensis / Q000201:enzymology* / Q000235:genetics*; D017433:Protein Structure, Secondary; D021381:Protein Transport; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics
D012333:RNA, Messenger; D020365:RNA Helicases
Leishmania; RNA helicase; Trypanosomatids; mRNA granules.