Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport.
Artificial ligands of streptavidin (ALiS) with association constants of ∼10(6) M(-1) were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.
Takuya
T
Terai
Moe
M
Kohno
Gaelle
G
Boncompain
Graduate School of Science, Osaka University , 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Shigeru
S
Sugiyama
Nae
N
Saito
Ryo
R
Fujikake
Tasuku
T
Ueno
Toru
T
Komatsu
Kenjiro
K
Hanaoka
Takayoshi
T
Okabe
Yasuteru
Y
Urano
Franck
F
Perez
Tetsuo
T
Nagano
D004353:Drug Evaluation, Preclinical; D042541:Intracellular Space / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D010539:Permeability; D011487:Protein Conformation; D021381:Protein Transport; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism*
D008024:Ligands; D019809:Streptavidin