Expression of hypoxia-inducible factor 1 alpha ameliorate myocardial ischemia in rat.
Hypoxia-inducible factor 1 alpha (HIF-1a), the upstream regulator of Vascular endothelial growth factor (VEGF), is the vital hypoxia related gene expression control factor. To evaluate HIF-1a therapeutic efficacy to acute myocardial infarction, the HIF-1a expressing recombinant Adeno-associated virus (rAAV) was constructed. The Wistar rat ischemic heart animal model was established with left anterior descending coronary artery ligation. The ischemic rats were treated with HIF-1a expressing and GFP expressing rAAVs respectively. Four weeks post the injection, the cardiac function of treated rat was compared by TM_WAVE system; size of infracted area was calculated by Evan's blue stain and capillary density was determined by CD31 immunohistochemical staining. Compare to the control group, the rats received HIF-1a expressing rAAV have smaller infracted heart size, the better heart function and higher capillary density than vehicle control group. The results show that the injection of HIF1a expressing rAAV can improve cardiac function and ameliorate acute myocardial ischemia (AMI) in rats.
Department of Cardiology, Hunan Provincial People's Hospital, Changsha, 410005, China. Electronic address: 2925772400@qq.com.
Peng
P
Jianqiang
Department of Cardiology, Hunan Provincial People's Hospital, Changsha, 410005, China.
Zhang
Z
Ping
Department of Cardiology, Hunan Provincial People's Hospital, Changsha, 410005, China.
Fan
F
Xinmin
Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Yuan
Y
Zhenhua
Department of Cardiology, Hunan Provincial People's Hospital, Changsha, 410005, China.
Zhou
Z
Ming
Department of Cardiology, Hunan Provincial People's Hospital, Changsha, 410005, China.
Guo
G
Ying
D000818:Animals; D002196:Capillaries / Q000473:pathology; D000229:Dependovirus / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000502:physiology / Q000627:therapeutic use*; D008297:Male; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D009204:Myocardial Revascularization; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use; D020257:Ventricular Remodeling
C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011994:Recombinant Proteins
Adeno-associate virus; Gene therapy; Hypoxia-inducible factor 1 alpha; Myocardial ischemia; Rat