Serum Dyslipidemia Is Induced by Internal Exposure to Strontium-90 in Mice, Lipidomic Profiling Using a Data-Independent Liquid Chromatography-Mass Spectrometry Approach.
Despite considerable research into the environmental risks and biological effects of exposure to external beam γ rays, incorporation of radionuclides has largely been understudied. This dosimetry and exposure risk assessment is challenging for first responders in the field during a nuclear or radiological event. Therefore, we have developed a workflow for assessing injury responses in easily obtainable biofluids, such as urine and serum, as the result of exposure to internal emitters cesium-137 ((137)Cs) and strontium-90 ((90)Sr) in mice. Here we report on the results of the untargeted lipidomic profiling of serum from mice exposed to (90)Sr. We also compared these results to those from previously published (137)Cs exposure to determine any differences in cellular responses based on exposure type. The results of this study conclude that there is a gross increase in the serum abundance of triacylglycerides and cholesterol esters, while phostaphatidylcholines and lysophosphatidylcholines displayed decreases in their serum levels postexposure at study days 4, 7, 9, 25, and 30, with corresponding average cumulative skeleton doses ranging from 1.2 ± 0.1 to 5.2 ± 0.73 Gy. The results show significant perturbations in serum lipidome as early as 2 days postexposure persisting until the end of the study (day 30).
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University , 3970 Reservoir Rd. NW, Washington, D.C. 20057, United States.
Maryam
M
Goudarzi
Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, New Mexico 87108, United States.
Waylon M
WM
Weber
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University , 3970 Reservoir Rd. NW, Washington, D.C. 20057, United States.
Juijung
J
Chung
Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, New Mexico 87108, United States.
Melanie
M
Doyle-Eisele
Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, New Mexico 87108, United States.
Dunstana R
DR
Melo
Mass Spectrometry Data Center, National Institute of Standards and Technology , 100 Bureau Drive, Gaithersburg, Maryland 20899, United States.
Tytus D
TD
Mak
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University , 3970 Reservoir Rd. NW, Washington, D.C. 20057, United States.
Steven J
SJ
Strawn
Center for Radiological Research, Columbia University , 630 West 168th Street, VC11-240, New York, New York 10032, United States.
David J
DJ
Brenner
Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, New Mexico 87108, United States.
Raymond
R
Guilmette
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University , 3970 Reservoir Rd. NW, Washington, D.C. 20057, United States.|Lombardi Comprehensive Cancer Center, Georgetown University , Washington, D.C. 20057, United States.
Albert J
AJ
Fornace
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D050171:Dyslipidemias / Q000097:blood* / Q000139:chemically induced*; D008055:Lipids / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D013326:Strontium Radioisotopes / Q000633:toxicity*
D008055:Lipids; D013326:Strontium Radioisotopes
UPLC; data-independent mass spectrometry; internal emitter; lipidomics; phospholipid; radiation exposure; strontium-90; triacylglycerol