Klotho gene polymorphisms are related to colorectal cancer susceptibility.
OBJECTIVE
The purpose of this study was to investigate the relationship of Klotho gene G-395A and C1818T polymorphisms with colorectal cancer (CRC) susceptibility.


METHODS
125 CRC patients and 125 controls were enrolled in the study. G-395A and C1818T polymorphisms were genotyped with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Haploview software was utilized to conduct linkage disequilibrium and haplotype analysis. Odds ratio (OR) and 95% confidence interval (95% CI) were used to analyze the correlation of genotypes and haplotypes with CRC susceptibility.


RESULTS
AA and GA genotypes of G-395A polymorphisms were related with CRC risk (AA: OR = 4.161, 95% CI = 1.437-12.053; GA: OR = 1.958, 95% CI = 1.133-3.385). The frequency of A allele was much higher in case group, compared with controls (31.2% vs.17.6%) and the value of OR AND 95% CI suggested that A allele served as a risk factor for CRC (OR = 2.123, 95% CI = 1.393-3.236). Haplotypes analysis indicated that A-C and A-T haplotypes were significantly associated with risk of CRC (OR = 1.822, 95% CI = 1.124-2.954; OR = 2.877, 95% CI = 1.340-6.176).


CONCLUSIONS
G-395A polymorphism of Klotho gene could increase the risk of CRC.
Department of Medical Oncology, The General Hospital of Beijing Military Area Beijing 100700, China.
Chang
C
Liu
Department of General Surgery, The General Hospital of Beijing Military Area Beijing 100700, China.
Wei
W
Cui
Department of Medical Oncology, The General Hospital of Beijing Military Area Beijing 100700, China.
Li
L
Wang
Health Department of Joint Service, Beijing Military Area Beijing 100043, China.
Lei
L
Yan
Department of Medical Oncology, The General Hospital of Beijing Military Area Beijing 100700, China.
Xinjian
X
Ruan
Department of Medical Oncology, The General Hospital of Beijing Military Area Beijing 100700, China.
Yanfang
Y
Liu
Department of Medical Oncology, The General Hospital of Beijing Military Area Beijing 100700, China.
Xiaoyan
X
Jia
Department of Medical Oncology, The General Hospital of Beijing Military Area Beijing 100700, China.
Xia
X
Zhang
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D000090265:Klotho Proteins; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
D005966:Glucuronidase; D000090265:Klotho Proteins
Klotho; colorectal cancer; polymorphisms