Tissue Factor Pathway Inhibitor-Coated Stents Inhibit Restenosis in a Rabbit Carotid Artery Model.
BACKGROUND
Our aim was to study the efficacy and safety of tissue factor pathway inhibitor (TFPI)-coated stents in inhibiting restenosis in a rabbit carotid artery model.


METHODS
Subculture was conducted in aorta smooth muscle cell, which was taken from male Wistar rat, and the 3-5-generation cells were taken for plasmid transfection and cytotoxicity experiment. TFPI microspheres were made of a TFPI plasmid which was enwrapped by poly-l-glutamic acid (PLGA). TFPI-coated stents (n = 7) and bare metal stents (n = 6) were implanted into prepared carotid artery stenosis model of New Zealand white rabbits. The transfection efficiency of TFPI gene and its influence on animal tissue, restenosis inhibition, and biochemical indicator were observed.


RESULTS
Tissue factor pathway inhibitor microspheres can transfect successfully into cells, and present no cytotoxicity. Autopsy results showed no pathological changes in liver and spleen of rabbits after implanting TFPI-coated stents. TFPI gene could transfect and express successfully in vessel wall cells, and thrombus was found in some lumens of bare metal stents group after 7 day, while no such thrombus was observed in coated stents group. Degree of hyperplasia of coronary endarterectomy in bare metal stents group was evidently higher than those in coated stents group. Obvious stent restenosis was discovered only in one case in bare metal stents group (diameter stenosis ≥50%). However, no case in coated stents group showed with stent restenosis.


CONCLUSIONS
Tissue factor pathway inhibitor-coated stents could successfully transfect TFPI gene into vessel wall cells, thereby inhibiting restenosis without obvious side effect in the rabbit carotid artery model.
Department of Cardiovasology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Mingyu
M
Shi
Department of Cardiovasology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Feng
F
Yin
Department of Cardiovasology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Hongyue
H
Gu
Department of Cardiovasology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Jing
J
Zhu
Department of Cardiovasology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Xinhua
X
Yin
D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology; D016893:Carotid Stenosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D057510:Endovascular Procedures / Q000295:instrumentation*; D015316:Genetic Therapy / Q000379:methods*; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D011474:Prosthesis Design; D011817:Rabbits; D017208:Rats, Wistar; D012008:Recurrence; D015607:Stents; D013997:Time Factors; D014162:Transfection
D020099:Coated Materials, Biocompatible; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor
Cell proliferation; Coated stents; Cytotoxicity; Pharmacokinetics; Poly-l-glutamic acid; Restenosis; Tissue factor pathway inhibitor