Biocompatible Collagen Paramagnetic Scaffold for Controlled Drug Release.
A porous collagen-based hydrogel scaffold was prepared in the presence of iron oxide nanoparticles (NPs) and was characterized by means of infrared spectroscopy and scanning electron microscopy. The hybrid scaffold was then loaded with fluorescein sodium salt as a model compound. The release of the hydrosoluble species was triggered and accurately controlled by the application of an external magnetic field, as monitored by fluorescence spectroscopy. The biocompatibility of the proposed matrix was also tested by the MTT assay performed on 3T3 cells. Cell viability was only slightly reduced when the cells were incubated in the presence of the collagen-NP hydrogel, compared to controls. The economicity of the chemical protocol used to obtain the paramagnetic scaffolds as well as their biocompatibility and the safety of the external trigger needed to induce the drug release suggest the proposed collagen paramagnetic matrices for a number of applications including tissue engeneering and drug delivery.
Department of Biological and Environmental Sciences and Technologies, DISTEBA, University of Salento , Via per Arnesano, I-73100 Lecce, Italy.
Simona
S
Bettini
Department of Engineering for Innovation, Campus University Ecotekne , Via per Monteroni, I-73100 Lecce, Italy.
Valentina
V
Bonfrate
Centre of Excellence for Nanostructured Materials (CENMAT), INSTM, Unit of Trieste, Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste , via L. Giorgieri 1, 34127, Trieste, Italy.
Zois
Z
Syrgiannis
Department of Engineering for Innovation, Campus University Ecotekne , Via per Monteroni, I-73100 Lecce, Italy.
Alessandro
A
Sannino
Department of Engineering for Innovation, Campus University Ecotekne , Via per Monteroni, I-73100 Lecce, Italy.
Luca
L
Salvatore
Department of Engineering for Innovation, Campus University Ecotekne , Via per Monteroni, I-73100 Lecce, Italy.
Marta
M
Madaghiele
Department of Biological and Environmental Sciences and Technologies, DISTEBA, University of Salento , Via per Arnesano, I-73100 Lecce, Italy.
Ludovico
L
Valli
Department of Cultural Heritage, University of Salento , Via Birago 64, I-73100 Lecce, Italy.
Gabriele
G
Giancane
D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology; D019793:Fluorescein / Q000737:chemistry / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*
D003692:Delayed-Action Preparations; D005290:Ferric Compounds; D020100:Hydrogels; C000499:ferric oxide; D003094:Collagen; D019793:Fluorescein