Inoculating plants with the endophytic bacterium Pseudomonas sp. Ph6-gfp to reduce phenanthrene contamination.
Plant organic contamination poses a serious threat to the safety of agricultural products and human health worldwide, and the association of endophytic bacteria with host plants may decrease organic pollutants in planta. In this study, we firstly determined the growth response and biofilm formation of endophytic Pseudomonas sp. Ph6-gfp, and then systematically evaluated the performance of different plant colonization methods (seed soaking (SS), root soaking (RS), leaf painting (LP)) for circumventing the risk of plant phenanthrene (PHE) contamination. After inoculation for 48 h, strain Ph6-gfp grew efficiently with PHE, oxalic acid, or malic acid as the sole sources of carbon and energy. Moreover, strain Ph6-gfp could form robust biofilms in LB medium. In greenhouse hydroponic experiments, strain Ph6-gfp could actively colonize inoculated plants internally, and plants colonized with Ph6-gfp showed a higher capacity for PHE removal. Compared with the Ph6-gfp-free treatment, the accumulations of PHE in Ph6-gfp-colonized plants via SS, RS, and LP were 20.1, 33.1, and 7.1 %, respectively, lower. Our results indicate that inoculating plants with Ph6-gfp could lower the risk of plant PHE contamination. RS was most efficient for improving PHE removal in whole plant bodies by increasing the cell numbers of Ph6-gfp in plant roots. The findings in this study provide an optimized method to strain Ph6-gfp reduce plant PAH residues, which may be applied to agricultural production in PAH-contaminated soil.
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing, 210095, China.
Kai
K
Sun
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing, 210095, China.
Juan
J
Liu
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing, 210095, China. gaoyanzheng@njau.edu.cn.
Yanzheng
Y
Gao
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing, 210095, China.
Yuehui
Y
Sheng
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing, 210095, China.
Fuxing
F
Kang
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing, 210095, China.
Michael Gatheru
MG
Waigi
D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000517:prevention & control; D006801:Humans; D018527:Hydroponics; D010616:Phenanthrenes / Q000032:analysis*; D018517:Plant Roots / Q000382:microbiology; D010945:Plants, Edible / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology*; D006109:Poaceae / Q000382:microbiology; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis*
D010616:Phenanthrenes; D012989:Soil Pollutants; C031181:phenanthrene
Colonization method; Endophytic Pseudomonas; Performance; Phenanthrene; Polycyclic aromatic hydrocarbons; Visualization