Identification of Bufavirus-1 and Bufavirus-3 in Feces of Patients with Acute Diarrhea, China.
Bufavirus (BuV) is a newly discovered human parvovirus that has been detected in some countries. The current study was designed to understand the epidemic of BuV in China. Totally 1877 fecal specimens were collected from pediatric and adult patients with acute diarrhea in two large hospitals from 2010 to 2014. BuV was detected in 0.5% (9/1877) of the fecal samples by PCR and subsequent sequencing. The positive patients had a wide age range from 1 month through 60 years (median 24 years old) and 6 were male. A geographic specific pattern was obvious, with significantly higher frequency of BuV presented in Northern than in Southern China. Four BuV-1 and five BuV-3 were determined. Mixed-infections of BuV with sapovirus and novavirus were found in 2 cases, respectively. A temporal clustering was identified, with most positive detection focused in the cold weather. These findings have expanded the current knowledge on the geographic boundaries of BuV circulation.
1] Graduate School of Anhui Medical University, 230032, Hefei, P. R. China [2] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Dou-Dou
DD
Huang
1] Graduate School of Anhui Medical University, 230032, Hefei, P. R. China [2] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Wei
W
Wang
School of Public Health, Peking University, 100191, Beijing, P. R. China.
Qing-Bin
QB
Lu
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Jin
J
Zhao
1] Graduate School of Anhui Medical University, 230032, Hefei, P. R. China [2] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Chen-Tao
CT
Guo
1] Graduate School of Anhui Medical University, 230032, Hefei, P. R. China [2] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Hong-Yu
HY
Wang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Xiao-Ai
XA
Zhang
1] Graduate School of Anhui Medical University, 230032, Hefei, P. R. China [2] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Yi-Gang
YG
Tong
1] Graduate School of Anhui Medical University, 230032, Hefei, P. R. China [2] State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Wei
W
Liu
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, P. R. China.
Wu-Chun
WC
Cao
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002681:China; D003710:Demography; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016013:Likelihood Functions; D008875:Middle Aged; D010802:Phylogeny; D029341:Sapovirus / Q000502:physiology*; D055815:Young Adult