Marine bacterial community structure resilience to changes in protist predation under phytoplankton bloom conditions.
To test whether protist grazing selectively affects the composition of aquatic bacterial communities, we combined high-throughput sequencing to determine bacterial community composition with analyses of grazing rates, protist and bacterial abundances and bacterial cell sizes and physiological states in a mesocosm experiment in which nutrients were added to stimulate a phytoplankton bloom. A large variability was observed in the abundances of bacteria (from 0.7 to 2.4 × 10(6) cells per ml), heterotrophic nanoflagellates (from 0.063 to 2.7 × 10(4) cells per ml) and ciliates (from 100 to 3000 cells per l) during the experiment (∼3-, 45- and 30-fold, respectively), as well as in bulk grazing rates (from 1 to 13 × 10(6) bacteria per ml per day) and bacterial production (from 3 to 379 μg per C l per day) (1 and 2 orders of magnitude, respectively). However, these strong changes in predation pressure did not induce comparable responses in bacterial community composition, indicating that bacterial community structure was resilient to changes in protist predation pressure. Overall, our results indicate that peaks in protist predation (at least those associated with phytoplankton blooms) do not necessarily trigger substantial changes in the composition of coastal marine bacterioplankton communities.
Centre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden.|Department of Marine Sciences, University of Otago, Dunedin, New Zealand.
Federico
F
Baltar
Centre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden.
Joakim
J
Palovaara
Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CSIC, Barcelona, Catalonia, Spain.
Fernando
F
Unrein
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Banyuls/mer, France.
Philippe
P
Catala
Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Hydrobiological Institute, České Budějovice, Czech Republic.
Karel
K
Horňák
Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Hydrobiological Institute, České Budějovice, Czech Republic.
Karel
K
Šimek
Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CSIC, Barcelona, Catalonia, Spain.
Dolors
D
Vaqué
Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CSIC, Barcelona, Catalonia, Spain.
Ramon
R
Massana
Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CSIC, Barcelona, Catalonia, Spain.
Josep M
JM
Gasol
Centre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden.
Jarone
J
Pinhassi
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D044822:Biodiversity; D056890:Eukaryota / Q000502:physiology*; D052836:Heterotrophic Processes; D010839:Phytoplankton / Q000254:growth & development*