Rapid start-up and microbial characteristics of partial nitrification granular sludge treating domestic sewage at room temperature.
The successful suppression of nitrite-oxidizing bacteria in the partial nitrification (PN) stage was the main challenge for the application of autotrophic nitrogen removal process treating mainstream sewage. In this study, two identical PN granular reactors (P1 and P2) were rapid started-up using the simultaneous PN and granulation strategy, for treating the domestic sewage. P1 was seeded with 30% PN granular sludge to induce nucleation, in which the granule size achieved to more than 400μm in 12d, with ammonia oxidation rate and nitrite accumulation rate of 80% and 95%, respectively, while P2 realized granulation in 42d. The presence of organic matters and specific structure of granules were profitable for the stability of PN for treating sewage with low ammonia. High-throughput pyrosequencing results indicated the biodiversity of both reactors decreased after start-up, and Nitrosomonas was the predominant specie of aerobic ammonia-oxidizing bacteria in PN granular sludge.
Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Yuhai
Y
Liang
Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: bjut_lidong@163.com.
Dong
D
Li
Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Huiping
H
Zeng
Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Cuidan
C
Zhang
Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Jie
J
Zhang
D019149:Bioreactors / Q000382:microbiology*; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism*; D009606:Nitrosomonas / Q000378:metabolism*; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology*; D013696:Temperature; D018508:Water Purification / Q000379:methods*
D009573:Nitrites; D012722:Sewage
Domestic sewage; Granular sludge; High-throughput pyrosequencing; Partial nitrification