Highly selective two-photon imaging of cysteine in cancerous cells and tissues.
Abnormal concentrations of Cys have been reported to be implicated in various health problems, including cancer, neuropathy, and cardiomyopathy. We present a novel two-photon fluorescent probe for the specific recognition of cysteine over homocysteine and glutathione, and the bioapplication of this probe for the imaging of live cancerous cells and thick tissues.
Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea. jongskim@korea.ac.kr.
Yun Hak
YH
Lee
Wen Xiu
WX
Ren
Jiyou
J
Han
Kyoung
K
Sunwoo
Ja-Yun
JY
Lim
Jong-Hoon
JH
Kim
Jong Seung
JS
Kim
D000818:Animals; D045744:Cell Line, Tumor; D003545:Cysteine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis; D006367:HeLa Cells; D056945:Hep G2 Cells; D006710:Homocysteine / Q000032:analysis; D006801:Humans; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology*; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D006710:Homocysteine; D005978:Glutathione; D003545:Cysteine