Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone.
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin. A concentration of 0.4% (w/v) RHP resulted in the greatest yield of prodigiosin (277.74 mg/L) after 48 h when mannitol was used as the sole source of carbon. The pigment yield was also influenced by the types of carbon sources and peptones. As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production. These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.
Atatürk University, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Esabi Basaran
EB
Kurbanoglu
Atatürk University, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey. ; Atatürk University, Department of Food, Ispir Hamza Polat Vocational School, Ataturk University, Erzurum, Turkey, Department of Food, Ispir Hamza Polat Vocational School, Ataturk University, Erzurum, Turkey.
Murat
M
Ozdal
Atatürk University, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Ozlem Gur
OG
Ozdal
Atatürk University, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Omer Faruk
OF
Algur
D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry*; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006110:Grasshoppers / Q000382:microbiology; D008969:Molecular Sequence Data; D010461:Peptones / Q000378:metabolism*; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011353:Prodigiosin / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012706:Serratia marcescens / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010461:Peptones; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D011353:Prodigiosin
Serratia marcescens; agricultural waste; prodigiosin; ram horn peptone