Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory.
Matrix-Assisted Laser Desorption Ionisation-Time of Flight (MALDI-ToF) Mass Spectrometry with Bruker MALDI Biotyper software was evaluated as a method for identifying veterinary bacteria. For 620 isolates (~100 bacterial species), identification by MALDI-ToF and non-16S rDNA methods (mainly phenotypic/biochemical) agreed to species-level (95.3%) and to species/genus-level (100%), but in the absence of 16S rDNA as a gold standard. For a further panel of 107 anaerobes and 234 aerobes (~100 bacteria species) using 16S rDNA results as the gold standard, MALDI-ToF/biochemical tests showed 97.8/96.6% species-level and 99.6/93.5% genus-level agreement for aerobes and 95.3/93.6% species-level and 100/95.3% genus-level agreement for anaerobes compared to the gold standard. Where results were obtained from direct spots, direct spots overlaid with formic acid and extracts, 89.4% of 180 aerobes and 90.1% of 152 anaerobes were identified by MALDI-ToF. MALDI-ToF was shown to be a rapid and reliable method to identify veterinary bacteria.
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Electronic address: luke.randall@apha.gsi.gov.uk.
Luke P
LP
Randall
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Fabrizio
F
Lemma
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Mark
M
Koylass
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Jon
J
Rogers
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Roger D
RD
Ayling
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Danny
D
Worth
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Monika
M
Klita
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Andrew
A
Steventon
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Kirsty
K
Line
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Peter
P
Wragg
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Jakub
J
Muchowski
Bruker Daltonik GmbH, Fahrenheitstr 4, D-28359 Bremen, Germany.
Markus
M
Kostrzewa
Animal and Plant Health Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Adrian M
AM
Whatmore
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis / Q000662:veterinary*; D005561:Formates; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000662:veterinary*; D014730:Veterinary Medicine / Q000379:methods*
D005561:Formates; D012336:RNA, Ribosomal, 16S; C030544:formic acid
16S rDNA; Bacterial identification; Biochemical; MALDI-ToF