Multi-lineage differentiation and angiogenesis potentials of pigmented villonodular synovitis derived mesenchymal stem cells--pathological implication.
Pigmented villonodular synovitis (PVNS) is a benign tissue proliferation characterized by its hyper-vascularity within the lesion. The true etiology and cell source of this disease entity still remain unclear. Mesenchymal stem cells (MSCs) exist in various tissues of human body. However, it has not been clarified whether MSCs could be isolated from tissue of PVNS. Here, we isolated MSCs from PVNS (PVNS-SCs), and by comparing to the MSCs from normal synovium (Syn-SCs) of the same individual, we investigated whether PVNS-SCs differed in the capacity for multi-differentiation and inducing angiogenesis. We first demonstrated that PVNS-SCs existed in the lesion of PVNS of three individuals. Moreover, we showed PVNS-SCs had better osteogenic differentiation potential than Syn-SCs, whereas Syn-SCs had better capacity for adipogenic and chondrogenic differentiation. By genome-wide analysis of gene expression profile using a complementary DNA microarray and comparing to Syn-SCs, we identified in PVNS-SCs a distinct gene expression profile characterized by up-regulation of genes involved in angiogenesis. In vitro and in vivo studies further confirmed that PVNS-SCs had better capacities for promoting angiogenesis. In summary, the identification of PVNS-SCs in PVNS tissue and their distinct angiogenic potential may help elucidate the underlying etiology of this disease.
Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
En-Rung
ER
Chiang
Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Hsiao-Li
HL
Ma
Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Jung-Pan
JP
Wang
Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Chien-Lin
CL
Liu
Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Tain-Hsiung
TH
Chen
Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.|Department of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.|Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.|Integrative Stem Cell Center, China Medical University, Taichung, Taiwan.
Shih-Chieh
SC
Hung
D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009389:Neovascularization, Pathologic; D013583:Synovial Membrane / Q000166:cytology*; D013586:Synovitis, Pigmented Villonodular / Q000209:etiology / Q000503:physiopathology*
angiogenesis; mesenchymal stem cells; microarray analysis; pigmented villonodular synovitis; synovium