Management of Hyperglycaemia by Ethyl Acetate Extract of Balanites aegyptiaca (Desert Date).
Reactive oxygen species play a significant role in the pathogenesis of retinopathy in diabetes patients. The current study aimed to assess the effect of ethyl acetate extract (EAE) from Balanites aegyptiaca (10, 25 or 50 mg/kg b.w.) in experimental diabetic rats. To achieve this aim, five groups of male rats were included: control, diabetic, and diabetic rats treated with 10, 25, and 50 µg/kg b.w. of EAE for eight weeks. Our results suggests a protective role of EAE against oxidative stress induced by streptozocine. EAE treatment produced a reduction in blood glucose levels, HbA1c, malondialdehyde and vascular endothelial growth factor (VEGF) in diabetic retina (p < 0.001), as well as an enhancement in antioxidant capacity against streptozocine-induced oxidative stress. Tumor necrosis factor alpha (TNF-α), interleukin (IL-1β) and vascular endothelial growth factor (VEGF) were significantly reduced in diabetic rats treated with EAE, compared with untreated diabetic rats. Analysis of EAE by GC-MS indicated the presence of β-sistosterol. Overall, EAE modulates oxidative stress induced by streptozocine and enhances antioxidant activity, which may provide additional endothelial protection in retina of diabetic rats. These results hold great promise in the management of diabetic complications.
Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. alalmalki@kau.edu.sa.|Bioactive Natural Products Research Group, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. alalmalki@kau.edu.sa.|Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. alalmalki@kau.edu.sa.
Abdulrahman L
AL
Al-Malki
Bioactive Natural Products Research Group, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Adjunct to Biochemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Agriculture Department, Faculty of Agricultural and Food Sciences, American University of Beirut, P.O. Box 11-0236, Beirut 1107 2020, Lebanon. kabualnaja@kau.edu.sa.
Elie K
EK
Barbour
Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Bioactive Natural Products Research Group, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.
Kalid O
KO
Abulnaja
Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Bioactive Natural Products Research Group, Faculty of Science, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80205, Jeddah 22254, Saudi Arabia. kabualnaja@kau.edu.sa.|Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt. kabualnaja@kau.edu.sa.
Said S
SS
Moselhy
D000085:Acetates / Q000737:chemistry; D000818:Animals; D032430:Balanites / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D003930:Diabetic Retinopathy / Q000097:blood / Q000188:drug therapy; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D000085:Acetates; D001786:Blood Glucose; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C007650:ethyl acetate
Balanites aegyptiaca; antidiabetic; ethyl acetate extract; inflammatory cytokines