Ingenane-type diterpene compounds from Euphorbia kansui modulate IFN-γ production through NF-κB activation.
BACKGROUND
Euphorbia kansui, a traditional medical herb, has been shown to have anti-tumour and anti-viral activities. Previously, we have reported that E. kansui increases interferon-gamma (IFN-γ) production in natural killer (NK) cells. However, it is not clear how E. kansui regulates IFN-γ secretion by NK cells.


RESULTS
In this study, E. kansui was separated into six individual compounds from the same chloroform fraction so that the activity of each compound could be compared. E. kansui compounds induced IFN-γ secretion through the phosphorylation of protein kinase D and IκB kinase pathways. Furthermore, E. kansui compounds activated the translocation of p65, a sub-unit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), to the nucleus and induced NF-κB at the transcriptional level.


CONCLUSIONS
These findings suggest that E. kansui enhances IFN-γ secretion through the NF-κB pathway in NK cells. © 2015 Society of Chemical Industry.
Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Sehyun
S
Oh
Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Hyun Woo
HW
Oh
Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Ha-Reum
HR
Lee
ENZYCHEM Lifesciences, 103-6, KAIST-ICC F741, Munjidong, Daejeon, 305-732, Republic of Korea.
Sun Young
SY
Yoon
Natural Medicine Researach Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gun, Chungbuk, Republic of Korea.
Sei-Ryang
SR
Oh
Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Young-Eun
YE
Ko
Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.|Department of Functional Genomics, Korea University of Science and Technology, Daejeon, 305-806, Republic of Korea.
Nina
N
Yoo
Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.|Department of Functional Genomics, Korea University of Science and Technology, Daejeon, 305-806, Republic of Korea.
Jinseon
J
Jeong
Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Jae Wha
JW
Kim
D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry*; D028481:Euphorbia / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000187:drug effects
D004224:Diterpenes; D016328:NF-kappa B; D007371:Interferon-gamma
Euphorbia kansui; IFN-gamma; NF-κB; NK cells