Flavivirus RNA synthesis in vitro.
Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.
Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC 20057, United States. Electronic address: rp55@georgetown.edu.
Radhakrishnan
R
Padmanabhan
Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC 20057, United States.
Ratree
R
Takhampunya
Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC 20057, United States.
Tadahisa
T
Teramoto
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, United States.
Kyung H
KH
Choi
D005416:Flavivirus / Q000235:genetics / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*
D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase
Cytoplasmic membrane-bound viral replication complex; Endogenous and exogenous viral RNA templates; Heparin trap; Polarity of RNA product by RNase H mapping; RNA-dependent RNA polymerase from positive strand mosquito-borne Flaviviridae; Temperature-dependent de novo initiation and elongation