[Candidatus "Jettenia moscovienalis" sp. nov., a New Species of Bacteria Carrying out Anaerobic Ammonium Oxidation].
A new species of bacteria oxidizing ammonium with nitrite under anoxic conditions was isolated from the activated sludge of a semi-industrial bioreactor treating digested sludge of the Kuryanovo wastewater treatment plant (Moscow, Russia). Physiological, morphological, and molecular genetic characterization of the isolate was carried out. The cells were ovoid (-0.5 x 0.8 μm), with the intracellular membrane structures characteristic of anammox bacteria (anammoxosome and paryphoplasm); unlike other anammox bacteria, it possessed extensive intracellular membrane structures located in layers parallel to the cytoplasmic membrane, but never close to the anammoxosome. The cells formed aggregates 5-28 μm in diameter and readily attached to solid surfaces. The cells were morphologically labile, easily plasmolyzed, and lost their content. Doubling time was 28 days, μ(max) = 0.025 day(-1); optimal temperature and pH for growth were 20-45 degrees C and 8.0, respectively. Phylogenetic analysis of the 16S rRNA gene sequences suggested its classification as a new species of the candidate genus Jettenia (order Planctomycetales). The name Candidatus "Jettenia moscovienalis" sp. nov. was proposed for the new bacterium.
A
A
Nikolaev
M N
MN
Kozlov
M V
MV
Kevbrina
A G
AG
Dorofeev
N V
NV
Pimenov
A Yu
AY
Kallistova
V A
VA
Grachev
E A
EA
Kazakova
A V
AV
Zharkov
B B
BB
Kuznetsov
E O
EO
Patutina
B K
BK
Bumazhkin
D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000648:ultrastructure; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D061328:Planctomycetales / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000382:microbiology; D014871:Water Microbiology
D000641:Ammonia; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D062065:Wastewater