Molecular application for identification of polycyclic aromatic hydrocarbons degrading bacteria (PAHD) species isolated from oil polluted soil in Dammam, Saud Arabia.
Soil contamination with petroleum hydrocarbon products such as diesel and engine oil is becoming one of the major environmental problems. This study describes hydrocarbons degrading bacteria (PHAD) isolated from long-standing petrol polluted soil from the eastern region, Dammam, Saudi Arabia. The isolated strains were firstly categorized by accessible shape detection, physiological and biochemistry tests. Thereafter, a technique established on the sequence analysis of a 16S rDNA gene was used. Isolation of DNA from the bacterial strains was performed, on which the PCR reaction was carried out. Strains were identified based on 16S rDNA sequence analysis, As follows amplified samples were spontaneously sequenced automatically and the attained results were matched to open databases. Among the isolated bacterial strains, S1 was identified as Staphylococcus aureus and strain S1 as Corynebacterium amycolatum.
King Saud University, Science College, Botany and Microbiology Department, P.O. Box 2455, Riyadh 11451, Saudi Arabia ; Alexandria University, Faculty of Science, Botany and Microbiology Department, P.O. Box 21511, Alexandria, Egypt.
Mohamed M
MM
Ibrahim
Al-Bassam Sch., Ministry of Education, P.O. Box 945, Dammam, Saudi Arabia.
Ameena
A
Al-Turki
Al-Bassam Sch., Ministry of Education, P.O. Box 945, Dammam, Saudi Arabia.
Dona
D
Al-Sewedi
King Saud University, Science College, Botany and Microbiology Department, P.O. Box 2455, Riyadh 11451, Saudi Arabia ; Prince Sultan Research Chair for Environment and Wild Life, King Saud University, Saudi Arabia.
Ibrahim A
IA
Arif
King Saud University, Science College, Botany and Microbiology Department, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Gehan A
GA
El-Gaaly
16S rDNA; Hydrocarbons; Molecular applications; Polluted soils