Helical Conjugated Polyelectrolyte Aggregation Induced by Biotin-Avidin Interaction.
Fluorescence correlation spectroscopy (FCS) is applied to demonstrate avidin-induced cross-linking in a system consisting of a helical anionic conjugated polyelectrolyte (P1) and a biotin-tetramethylrhodamine (TMR) conjugate (2). In a previous study, we used fluorescence spectroscopy to demonstrate that 2 binds to P1 via intercalation of the TMR chromophore into the P1 helix. Addition of avidin to the P1/2 complex induces little change in the fluorescence of the system; however, FCS reveals a remarkable increase in the diffusion time of the P1/2 complex in the presence of avidin. This change is attributed to supramolecular polymer aggregates produced by cross-link formation between the biotin unit of intercalated 2 and avidin. Atomic force microscopy imaging provides evidence supporting the existence of these aggregates. The highly sensitive FCS method is used to develop a novel sensor for the biotin-avidin interaction, with a detection limit of <100 pM for avidin.
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
Danlu
D
Wu
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
Fude
F
Feng
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
Dongping
D
Xie
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
Yan
Y
Chen
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
Weihong
W
Tan
Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
Kirk S
KS
Schanze
aggregation; avidin−biotin; conjugated polyelectrolyte; fluorescence correlation spectroscopy