In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction () and Vascular Endothelial Growth Factor: A Comparison Study.
OBJECTIVE
To compare the angiogenesis behaviors of vascular endothelial growth factor (VEGF) and Chinese medicine Xuefu Zhuyu Decoction (, XZD) treatments.


METHODS
Human microvascular endothelial cells (HMEC-1) were treated with various concentrations of either XZD-containing serum (XZD-CS) or VEGF for 24, 48, and 72 h, respectively. Cell viability, proliferation, migration, adhesion, and in vitro tube formation assays were used to assess their angiogenic effects.


RESULTS
VEGF promoted all cellular phases involved in angiogenesis including cell viability, proliferation, migration, adhesion, and tube formation (<0.05 or <0.01). Unlike the continuous promotion effects of VEGF at the above stages, XZD inhibited cell viability and proliferation (<0.05 or <0.01) and only promoted tube formation in the early phase of angiogenesis (<0.01).


CONCLUSIONS
These two medications promote different angiogenesis behaviors, which might be an important reason for their distinct therapeutic profile in clinical usage.
College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
Fan
F
Lin
The Second Hospital of Longyan, Longyan, Fujian Province, 364000, China.
Bin-Ling
BL
Chen
College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
Yi-Zheng
YZ
Wang
College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. 976558160@qq.com.
Dong
D
Gao
Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China. junsong86@sohu.com.
Jun
J
Song
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA.
T J
TJ
Kaptchuk
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Ke-Ji
KJ
Chen
D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D055806:Microvessels / Q000166:cytology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A; C513826:Xue-Fu-Zhu-Yu decoction
Chinese medicine; Xuefu Zhuyu Decoction; angiogenesis; vascular endothelial growth factor