Effect of Doxycycline in Gel Form on Bone Regeneration: Histomorphometric and Tomographic Study in Rat Calvaria.
BACKGROUND
This study aims to investigate the effects of the local application of doxycycline in the form of natrosol-based gel on bone regeneration by examining critical defects in rat calvaria.


METHODS
Twenty-four rats were randomly divided into three groups with eight animals each, according to the treatment received: BC (untreated, filled with blood clot), NAT (natrosol gel alone), and DOX (10% doxycycline gel). Four animals from each group were euthanized at 4 and 8 weeks postoperatively. In tomographic analysis, mean density in the region of the defect was calculated as a percentage relative to the native bone density. In histomorphometric analysis, the newly formed bone area was calculated as a percentage of the total area. The values obtained underwent analysis of variance and Tukey testing (P <0.05).


RESULTS
The mean percentage of tomographic density in the region of the defect at the end of 8 weeks was higher for DOX (44.37%), and statistical differences in this period were observed between DOX and NAT (33.10%) and DOX and BC (32.43%). Regarding new bone formation, at the end of 8 weeks, DOX (61.11%) also had the highest mean bone formation, and statistical differences were observed between DOX and NAT (34.61%) and DOX and BC (23.11%).


CONCLUSIONS
It was observed that 10% doxycycline gel had a good effect on bone regeneration regarding the filling of critical defects in rat calvaria.
Department of Clinic and Surgery, Federal University of Alfenas, Alfenas, MG, Brazil.
Amanda C
AC
Silva
Department of Diagnostics and Surgery, São Paulo Estadual University, Araraquara, SP, Brazil.
Marina R
MR
Oliveira
Department of Clinic and Surgery, Federal University of Alfenas, Alfenas, MG, Brazil.
Lysandro F A
LF
Amaral
Department of Surgery and Clinical Integrated, São Paulo Estadual University, Araçatuba, SP, Brazil.
Sabrina
S
Ferreira
Department of Surgery and Clinical Integrated, São Paulo Estadual University, Araçatuba, SP, Brazil.
Idelmo R
IR
Garcia
Department of Clinic and Surgery, Federal University of Alfenas, Alfenas, MG, Brazil.
Ronaldo C
RC
Mariano
D000818:Animals; D001861:Bone Regeneration; D004318:Doxycycline; D010012:Osteogenesis; D051381:Rats; D012886:Skull
D004318:Doxycycline
Biocompatible materials; Bone regeneration; Cone-beam computed tomography; Doxycycline; Tissue Scaffolds; tissue engineering