Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes.
An important function of the liver is the synthesis and secretion of blood coagulation factors. Within the liver, hepatocytes are involved in the synthesis of most blood coagulation factors, such as fibrinogen, prothrombin, factor V, VII, IX, X, XI, XII, as well as protein C and S, and antithrombin, whereas liver sinusoidal endothelial cells produce factor VIII and von Willebrand factor. Here, we describe methods for the detection and quantification of most blood coagulation factors in hepatocytes in vitro. Hepatocyte cultures indeed provide a valuable tool to study blood coagulation factors. In addition, the generation and expansion of hepatocytes or hepatocyte-like cells may be used in future for cell-based therapies of liver diseases, including blood coagulation factor deficiencies.
Medicyte GmbH, Im Neuenheimer Feld 581, 69120, Heidelberg, Germany, s.heinz@medicyte.com.
Stefan
S
Heinz
Joris
J
Braspenning
D001779:Blood Coagulation Factors / Q000096:biosynthesis*; D001780:Blood Coagulation Tests / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D061251:Primary Cell Culture / Q000379:methods
D001779:Blood Coagulation Factors