ZnO Nanoparticles Upregulates Adipocyte Differentiation in 3T3-L1 Cells.
The present study was aimed to investigate the effect of zinc oxide (ZnO) nanoparticles on 3T3-L1 cell differentiation, by quantitating peroxisome proliferators-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid binding protein 4 (FABP4), sterol regulatory element-binding transcription factor 1 (SREBP1), and serine-threonine kinase cyclin-dependent kinase 4 (cdk4), which are critical for adipogenesis. 3T3-L1 preadipocyte cells were cultured and differentiated with the standard differentiation medium. Sulforhodamine B (SRB) assay determined 3T3-L1 cell viability. ZnO nanoparticles increased the lipid accumulation in differentiated adipocytes as evidenced by Oil Red O staining. The quantitative PCR (qPCR) analysis showed that the PPARγ, FABP4, C/EBPα, and SREBP1 messenger RNA (mRNA) expression was significantly increased in the ZnO nanoparticle-treated 3T3-L1 adipocytes. Western blot analysis showed increased PPARγ, FABP4, C/EBPα, and SREBP1 protein expression compared to their respective controls. Also, the immunofluorescence study showed the increased cdk4 and PPARγ expression in the nanoparticle-treated cells. Taking all these data together, it is concluded that ZnO nanoparticles may be a potent substance to alter 3T3-L1 preadipocyte differentiation and adipogenesis.
Department of Bioresources and Food Sciences, Konkuk University, Seoul, Republic of Korea.
Muthuraman
M
Pandurangan
Department of Bioresources and Food Sciences, Konkuk University, Seoul, Republic of Korea. byj@sunbio2.com.
Bong Yeon
BY
Jin
Department of Bioresources and Food Sciences, Konkuk University, Seoul, Republic of Korea. frenzram1980@gmail.com.
Doo Hwan
DH
Kim
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D015034:Zinc Oxide / Q000737:chemistry*
D012333:RNA, Messenger; D015034:Zinc Oxide
3T3-L1 cells; Differentiation; Lipid accumulation; SRB assay; ZnO nanoparticles