Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.
Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Jia-le
JL
He
Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Jia-Wei
JW
Zhao
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Zeng-Chun
ZC
Ma
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Yu-Guang
YG
Wang
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Qian-de
QD
Liang
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Hong-Ling
HL
Tan
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Cheng-Rong
CR
Xiao
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Xiang-Lin
XL
Tang
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.
Yue
Y
Gao