A Lactobacillus plantarum strain isolated from kefir protects against intestinal infection with Yersinia enterocolitica O9 and modulates immunity in mice.
Lactobacillus plantarum C4, previously isolated from kefir and characterized as a potential probiotic strain, was tested for its protective and immunomodulatory capacity in a murine model of yersiniosis. The inoculation of BALB/c mice with a low pathogenicity serotype O9 strain of Yersinia enterocolitica results in a prolonged intestinal infection with colonization of Peyer's patches. Pretreatment with C4 was without effect on fecal excretion of yersiniae, but shortened the colonization of Peyer's patches. This protective effect was associated with pro-inflammatory status in the intestinal mucosa (TNF-α production in infected mice was increased by C4) and an increase in total IgA secretion. At a systemic level, C4 did not promote a pro-inflammatory response, although production of the immunoregulatory cytokine IFN-γ was enhanced. These findings suggest that L. plantarum C4 can increase resistance to intestinal infections through its immunomodulatory activity.
Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain. Electronic address: soumidemontijo@gmail.com.
Soumi
S
De Montijo-Prieto
Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain. Electronic address: morenoc@ugr.es.
Encarnación
E
Moreno
Department of Nutrition and Bromatology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain. Electronic address: trianab@ugr.es.
Triana
T
Bergillos-Meca
Biotmicrogren S. L., Parque tecnológico de Ciencias de la Salud, BIC nave 6, 18100, Armilla, Granada, Spain. Electronic address: direccion@biot.es.
Agustín
A
Lasserrot
Department of Nutrition and Bromatology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain. Electronic address: mdruiz@ugr.es.
María-Dolores
MD
Ruiz-López
Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain. Electronic address: aruizbr@ugr.es.
Alfonso
A
Ruiz-Bravo
Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain. Electronic address: mjvalera@ugr.es.
María
M
Jiménez-Valera
D000818:Animals; D043302:Cultured Milk Products / Q000382:microbiology*; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D056747:Immunomodulation; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology; D019936:Probiotics; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D015009:Yersinia Infections / Q000276:immunology / Q000517:prevention & control*; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000276:immunology
D016207:Cytokines; D007071:Immunoglobulin A, Secretory; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
Cytokines; Immunomodulation; Infection; Lactobacillus plantarum; Probiotic; Yersinia enterocolitica