In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens.
Among 360 isolates from the gastrointestinal tract (GIT) of broilers, eleven isolates which showed in vitro probiotic properties were identified and selected for further tests. After the in vitro screening, three strains were chosen for the in vivo study of persistence of fresh cultures and then one strain was selected for the in vivo study of persistence of lyophilized culture. Lyophilized Lactobacillus salivarius DSPV 001P was capable of persisting in broilers during a complete rearing, even 28 days following cessation of administration. L. salivarius DSPV 001P administered to broilers and recovered from GIT was compared by pulsed-field gel electrophoresis (PFGE) to ensure that the same genotype was persistently identified. A combination of in vitro and in vivo screening of native lactic acid bacteria (LAB) described in this study may offer a method for selecting the most suitable strain for potential application as a broiler probiotic supplement.
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Jesica
J
Blajman
Department of Public Health, Faculty of Veterinary Science, National University of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Cristian
C
Gaziano
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina; Department of Public Health, Faculty of Veterinary Science, National University of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina. Electronic address: virginiazbrun@yahoo.com.ar.
María Virginia
MV
Zbrun
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina; Department of Public Health, Faculty of Veterinary Science, National University of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Lorena
L
Soto
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Diego
D
Astesana
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Ayelén
A
Berisvil
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Analía Romero
AR
Scharpen
Department of Public Health, Faculty of Veterinary Science, National University of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina; National Council of Scientific and Technical Research (CONICET), National Institute of Agricultural Technology EEA Rafaela, Ruta 34 Km 227 (2300), Rafaela, Santa Fe, Argentina.
Marcelo
M
Signorini
National Council of Scientific and Technical Research (ICIVET-CONICET/UNL), Laboratory of Food Analysis, Institute of Veterinary Science of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina; Department of Public Health, Faculty of Veterinary Science, National University of the Littoral, Kreder 2805 (S3080HOF), Esperanza, Santa Fe, Argentina.
Laureano
L
Frizzo
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D002645:Chickens / Q000254:growth & development / Q000382:microbiology*; D055808:Drug Discovery / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005612:Freeze Drying / Q000662:veterinary; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005838:Genotype; D066298:In Vitro Techniques / Q000662:veterinary; D007778:Lactobacillus / Q000235:genetics*; D019936:Probiotics / Q000592:standards*
Broilers; Intestinal microbiota; Lactic acid bacteria screening; Probiotic