Accurate Identification and Selective Removal of Rotavirus Using a Plasmonic-Magnetic 3D Graphene Oxide Architecture.
According to the World Health Organization, even in the 21st century, more than one million children die each year due to the rotavirus contamination of drinking water. Therefore, accurate identification and removal of rotavirus are very important to save childrens' lives. Driven by the need, in this Letter, we report for the first time highly selective identification and removal of rotavirus from infected water using a bioconjugated hybrid graphene oxide based three-dimensional (3D) solid architecture. Experimental results show that due to the presence of a high intensity of "hot spots" in the 3D network, an antibody-attached 3D plasmonic-magnetic architecture can be used for accurate identification of rotavirus using surface-enhanced Raman spectroscopy (SERS). Reported data demonstrate that the antibody-attached 3D network binds strongly with rotavirus and is capable of highly efficient removal of rotavirus, which has been confirmed by SERS, fluorescence imaging, and enzyme-linked immunosorbent assay (ELISA) data. We discuss a possible mechanism for accurate identification and efficient removal of rotavirus from infected drinking water.
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Zhen
Z
Fan
‡Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, Texas 78249, United States.
Brian
B
Yust
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Bhanu Priya Viraka
BP
Nellore
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Sudarson Sekhar
SS
Sinha
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Rajashekhar
R
Kanchanapally
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Rebecca A
RA
Crouch
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Avijit
A
Pramanik
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Suhash Reddy
SR
Chavva
‡Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, Texas 78249, United States.
Dhiraj
D
Sardar
†Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
Paresh Chandra
PC
Ray
SERS and fluorescence imaging; label-free rotavirus identification; plasmonic−magnetic 3D graphene oxide architecture; selective separation of rotavirus