Quantification of viral genome in cord blood donors by real time PCR to investigate human herpesvirus type 8 active infection.
Umbilical cord blood (UCB) is one of the most important sources of hematopoietic stem cells which can be used for transplantation. The transplanted CB stem cells might cause infections in recipients. The aim of this study is to evaluate Human Herpes Virus8 (HHV8) as a Rhadinovirus among CB samples in order to assess safety of cord blood stem cells transplantation. To assess this aim, we surveyed 800 cord blood specimens by Real Time PCR.The overall HHV8 incidence in cord blood mononuclear cells was 1.38% and none of them was in lytic phase of HHV8. The authors suggest further HHV8 study on CB samples for transplantation.
Department of Immunology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Neda
N
Golchin
Department of Immunology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address: m.kheirandish@ibto.ir.
Maryam
M
Kheirandish
Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Zohreh
Z
Sharifi
Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Shahram
S
Samiee
Cancer Research Center and Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran.
Parviz
P
Kokhaei
Department of Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Zahra
Z
Pourpak
D001782:Blood Donors; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005312:Fetal Blood / Q000821:virology*; D016679:Genome, Viral; D019288:Herpesvirus 8, Human; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
Cord blood; HHV8; Real time PCR