A tubular gelatin scaffold capable of the time-dependent controlled release of epidermal growth factor and mitomycin C.
A tubular gelatin scaffold for the time-dependent controlled release of epidermal growth factor (EGF) and mitomycin C (MMC) was fabricated. EGF was incorporated using silk fibroin carriers, and MMC was planted using polylactide (PLA) microspheres. The relationship between scaffold properties and crosslinking degrees was evaluated. As the crosslinking degree was increased from 23.7% to 65.3%, the mechanical properties of the scaffold obviously improved, and the compressive modulus increased to approximately 65kPa. The mass degradation of the scaffold was also controlled from 9 days to approximately 1 month. In vitro release tests indicated that the scaffold mainly released EGF in the early period and MMC in the later period. Urethral epithelial cells (UECs) and urethral scar derived fibroblast cells (UFCs) were coseeded in the scaffold at a ratio of 1:1. After 9 days of coculture, immunostaining results displayed that the proportion of UECs continuously increased to approximately 71%. These changes in cell proportion were confirmed by the results of Western blot analysis. Therefore, the scaffold promoted the growth but inhibited the regeneration of UFCs. This scaffold for time-dependent controlled release of multiple biofactors may be potentially useful in urethral reconstruction and other tissue engineering studies.
Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510182, China. Electronic address: zhujixiang888@126.com.
Jixiang
J
Zhu
Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510182, China. Electronic address: yangfanwen@163.com.
Fanwen
F
Yang
Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510182, China. Electronic address: wild.4145@163.com.
Fupo
F
He
Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510182, China. Electronic address: tianxiumei224@163.com.
Xiumei
X
Tian
Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address: tangshuo1205@163.com.
Shuo
S
Tang
Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510182, China. Electronic address: xmchenw@126.com.
Xiaoming
X
Chen
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D018920:Coculture Techniques; D003692:Delayed-Action Preparations; D004815:Epidermal Growth Factor / Q000008:administration & dosage* / Q000737:chemistry; D004847:Epithelial Cells / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005349:Fibroins; D005780:Gelatin / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage* / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects; D054457:Tissue Scaffolds; D014521:Urethra / Q000166:cytology / Q000187:drug effects / Q000254:growth & development
D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D016685:Mitomycin; D004815:Epidermal Growth Factor; D005780:Gelatin; D005349:Fibroins
Controlled release; Epidermal growth factor; Gelatin scaffold; Mitomycin C