Protective Effect of Isorhamnetin on Lipopolysaccharide-Induced Acute Lung Injury in Mice.
Isorhamnetin has been reported to have anti-inflammatory, anti-oxidative, and anti-proliferative effects. The aim of this study was to investigate the protective effect of isorhamnetin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice by inhibiting the expression of cyclooxygenase-2 (COX-2). The effects of isorhamnetin on LPS-induced lung pathological damage, wet/dry ratios and the total protein level in bronchoalveolar lavage fluid (BALF), inflammatory cytokine release, myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA) level were examined. In addition, the COX-2 activation in lung tissues was detected by Western blot. Isorhamnetin pretreatment improved the mice survival rates. Moreover, isorhamnetin pretreatment significantly attenuated edema and the pathological changes in the lung and inhibited protein extravasation in BALF. Isorhamnetin also significantly decreased the levels of inflammatory cytokines in BALF. In addition, isorhamnetin markedly prevented LPS-induced oxidative stress. Furthermore, isorhamnetin pretreatment significantly suppressed LPS-induced activation of COX-2. Isorhamnetin has been demonstrated to protect mice from LPS-induced ALI by inhibiting the expression of COX-2.
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Bo
B
Yang
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Xiao-Ping
XP
Li
Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, No. 1 XinSi Road, Xi'an, 710038, People's Republic of China.
Yun-Feng
YF
Ni
Department of Anesthesiology, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Hong-Yin
HY
Du
School of Aerospace, Xi'an Jiaotong University, No. 28 Xianning West Road, Xi'an, 710049, People's Republic of China.
Rong
R
Wang
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Ming-Jiang
MJ
Li
Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, No. 1 XinSi Road, Xi'an, 710038, People's Republic of China.
Wen-Chen
WC
Wang
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Ming-Ming
MM
Li
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Xu-Hui
XH
Wang
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China.
Lei
L
Li
Department of Thoracic Surgery, Tianjin First Center Hospital, No. 24 FuKang Road, Tianjin, 300192, People's Republic of China. zhangweidongchest@163.com.
Wei-Dong
WD
Zhang
Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, No. 1 XinSi Road, Xi'an, 710038, People's Republic of China. jiangtaochest@163.com.
Tao
T
Jiang
D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000401:mortality; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D011654:Pulmonary Edema / Q000188:drug therapy; D011794:Quercetin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C047368:3-methylquercetin; D008315:Malondialdehyde; D011794:Quercetin; D009195:Peroxidase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D013482:Superoxide Dismutase
ALI; COX-2; LPS; isorhamnetin