Protective effects of probiotic Lactobacillus plantarum BJ0021 on liver and kidney oxidative stress and apoptosis induced by endosulfan in pregnant rats.
Endosulfan (EDS) is one of the most widely organochlorine insecticide used in many parts of the world, although it is currently banned or severely restricted in use in some countries. EDS causes a variety of negative effects in non-target species including humans. Therefore, the aim of the present study was to investigate the possible protective effects of Lactobacillus plantarum BJ0021 on toxicity, oxidative stress, and apoptosis induced by EDS intoxication on liver and kidneys of pregnant rats. This pesticide induced a significant increase in total cholesterol, alanine-amino transferase (ALAT), aspartate-amino transferase (ASAT), alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), urea and creatinine in serum, while urinary urea and creatinine were lower than those of the control group. In the liver and kidney, lipid peroxidation increased significantly, the antioxidant levels, such as superoxide dismutase (SOD) and catalase (CAT) were markedly depressed and TdT-mediated dUTP-biotin Nick End Labeling (TUNEL) revealed more apoptotic cells. In contrast, co-administration of L. plantarum BJ0021 to EDS-treated animals ameliorated most of these biochemical parameters, but the activity of antioxidant enzymes (SOD, CAT) did not modify and the number of apoptotic nuclei remained significantly raised in kidney compared to control. In conclusion, the administration of L. plantarum BJ0021 decreased apoptosis and might play a protective role in reducing toxicity of EDS in pregnant rats.
a Department of Natural and Life Science, Faculty of Science, Laboratory of Molecular Toxicology , University of Jijel , Jijel , Algeria .
Leila
L
Bouhafs
b Laboratoire de Biologie Générale , Université de Lyon , Cedex , France .|c Laboratoire de Reproduction et Développement Comparé , Université Catholique de Lyon, Ecole Pratique des Hautes Etudes , Cedex , France , and.
Elara N
EN
Moudilou
b Laboratoire de Biologie Générale , Université de Lyon , Cedex , France .
Jean Marie
JM
Exbrayat
a Department of Natural and Life Science, Faculty of Science, Laboratory of Molecular Toxicology , University of Jijel , Jijel , Algeria .
Mesbah
M
Lahouel
a Department of Natural and Life Science, Faculty of Science, Laboratory of Molecular Toxicology , University of Jijel , Jijel , Algeria .|d Laboratory of Biotechnology, Environment and Health, Faculty of Science , University of Jijel , Algeria.
Tayeb
T
Idoui
D000818:Animals; D000975:Antioxidants / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000652:urine; D004726:Endosulfan / Q000633:toxicity*; D005260:Female; D020287:In Situ Nick-End Labeling / Q000379:methods; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects*; D007677:Kidney Function Tests; D048191:Lactiplantibacillus plantarum; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D019936:Probiotics / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014508:Urea / Q000652:urine; D005723:gamma-Glutamyltransferase / Q000097:blood
D000975:Antioxidants; D007306:Insecticides; D008315:Malondialdehyde; D014508:Urea; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D004726:Endosulfan
Apoptosis; Bacillus plantarum; EDS; oxidative stress; pregnant rats