A RAD-based phylogenetics for Orestias fishes from Lake Titicaca.
The fish genus Orestias is endemic to the Andes highlands, and Lake Titicaca is the centre of the species diversity of the genus. Previous phylogenetic studies based on a single locus of mitochondrial and nuclear DNA strongly support the monophyly of a group composed of many of species endemic to the Lake Titicaca basin (the Lake Titicaca radiation), but the relationships among the species in the radiation remain unclear. Recently, restriction site-associated DNA (RAD) sequencing, which can produce a vast number of short sequences from various loci of nuclear DNA, has emerged as a useful way to resolve complex phylogenetic problems. To propose a new phylogenetic hypothesis of Orestias fishes of the Lake Titicaca radiation, we conducted a cluster analysis based on morphological similarities among fish samples and a molecular phylogenetic analysis based on RAD sequencing. From a morphological cluster analysis, we recognised four species groups in the radiation, and three of the four groups were resolved as monophyletic groups in maximum-likelihood trees based on RAD sequencing data. The other morphology-based group was not resolved as a monophyletic group in molecular phylogenies, and some members of the group were diverged from its sister group close to the root of the Lake Titicaca radiation. The evolution of these fishes is discussed from the phylogenetic relationships.
Department of Zoology, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan; National Institute of Genetics, Yata, Mishima, Shizuoka 411-8540, Japan. Electronic address: tetsumi@hitohaku.jp.
Tetsumi
T
Takahashi
Universidad Nacional del Altiplano, Ave. Sesquicentenario No. 1150, Puno, Peru.
Edmundo
E
Moreno
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D001838:Bolivia; D016000:Cluster Analysis; D005399:Fishes / Q000235:genetics*; D060106:Lakes; D010568:Peru; D010802:Phylogeny; D017422:Sequence Analysis, DNA
Adaptive radiation; Altiplano; Ancient lake; Cluster analysis; Maximum-likelihood analysis; Restriction site-associated DNA