Nigella sativa amliorates inflammation and demyelination in the experimental autoimmune encephalomyelitis-induced Wistar rats.
Multiple sclerosis (MS) is the major, immune-mediated, demyelinating neurodegenerative disease of the central nervous system (CNS). Experimental autoimmune encephalomyelitis (EAE) is a well-established animal model of MS. The aim of the present study was to investigate the protective and ameliorative effects of N. sativa seeds (2.8 g/kg body weight) in EAE-induced Wistar rats. EAE-induced rats were divided into: 1- EAE-induced rats ("EAE" group). 2- "N. sativa + EAE" group received daily oral administration of N. sativa 2 weeks prior EAE induction until the end of the experiment. 3- "EAE + N. sativa" group received daily oral administration of N. sativa after the appearance of first clinical signs until the end of the experiment. All animals were decapitated at the 28th day post EAE-induction. EAE was investigated using histopathological, immunohistochemical and ultrastructural examinations in addition to determination of some oxidative stress parameters in the cerebellum and medulla. N. sativa suppressed inflammation observed in EAE-induced rats. In addition, N. sativa enhanced remyelination in the cerebellum. Moreover, N. sativa reduced the expression of transforming growth factor beta 1 (TGF β1). N. sativa seeds could provide a promising agent effective in both the protection and treatment of EAE.
Department of Zoology, Faculty of Science, Cairo University Giza, Egypt.
Neveen A
NA
Noor
Department of Biophysics, Faculty of Science, Cairo University Giza, Egypt.
Heba M
HM
Fahmy
Department of Pathology, Faculty of Veterinary Medicine, Cairo University Giza, Egypt.
Faten F
FF
Mohammed
Department of Biophysics, Faculty of Science, Cairo University Giza, Egypt.
Anwar A
AA
Elsayed
Department of Zoology, Faculty of Science, Cairo University Giza, Egypt.
Nasr M
NM
Radwan
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007249:Inflammation; D046529:Microscopy, Electron, Transmission; D009186:Myelin Sheath / Q000187:drug effects* / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D031881:Nigella sativa; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds
D018696:Neuroprotective Agents; D010936:Plant Extracts
Experimental autoimmune encephalomyelitis (EAE); Nigella sativa (N. sativa); multiple sclerosis (MS); oxidative stress; transforming growth factor beta 1 (TGF-β1)