[Fuzheng Huayu recipe promotes the fenestration of capillarization in liver sinusoidal endothelial cells].
OBJECTIVE
To explore the effect of Fuzheng Huayu (FZHY) recipe on the fenestration of capillarization in liver sinusoidal endothelial cells (LSECs).


METHODS
Ten Sprague Dawley (SD) rats were fed with 0.46 g/kg FZHY powder by intragastric administration. Two hours later, a second gavage were given to the rats. The serum from rat heart at 1 hour after second gavage was collected (FZHY group, n=10). Another ten SD rats was administrated with distilled water through the same process and served as the control (control group, n=10). The serum from both groups were separately diluted with Dulbecco minimum essential medium (DMEM) for 10% and served as the culture medium for LSECs. At the different conditions, the vWF and CD31 expressions were detected by immunocytochemistry and Western blot, while the changes of LSECs fenestrae structure were observed under scanning electron microscopy.


RESULTS
1) Immunocytochemistry and Western blot showed that the vWF and CD31 protein levels were lower in LSECs in the FZHY group than those in the control group. The gray levels of vWF and CD31 protein were 0.548±0.020 and 0.262±0.010 in the FZHY group, and 0.845±0.090 and 0.383±0.010 in the control group respectively, with statistical significant difference (t=5.18, 9.61, both P<0.05). 2) The results from scanning electron microscopy showed that the fenestration of LSECs was closed and almost lost in the control group, but many fenestra appeared in the LSECs in the FZHY group.


CONCLUSIONS
FZHY recipe can suppress the expression of vWF and CD31, increase the fenestrae on the LSECs surface and reverse the capillarization of LSECs.
Department of Infectious Diseases, First people's Hospital of Huaihua, Huaihua Hunan 418000, China.
Zhibing
Z
Xie
Department of Pathology,  Xiangya Hospital, Central South University, Changsha 410008, China.
Yanqing
Y
Yu
Department of Pathology,  Xiangya Hospital, Central South University, Changsha 410008, China.
Deyun
D
Feng
Department of Infectious Diseases, Xiangya Hospital,  Central South University, Changsha 410008, China.
Jianping
J
Xie
Department of Infectious Diseases, Xiangya Hospital,  Central South University, Changsha 410008, China.
Fei
F
Liu
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D008099:Liver / Q000166:cytology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014841:von Willebrand Factor / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C532829:fuzheng huayu; D014841:von Willebrand Factor