Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge.
Advances in noninvasive imaging modalities have provided opportunities to study β cell function through imaging zinc release from insulin secreting β cells. Understanding the temporal secretory pattern of insulin and zinc corelease after a glucose challenge is essential for proper timing of administration of zinc sensing probes. Portal venous sampling is an essential part of pharmacological and nutritional studies in animal models. The purpose of this study was to compare two different percutaneous image-guided techniques: transhepatic ultrasound guided portal vein access and transsplenic fluoroscopy guided splenic vein access for ease of access, safety, and evaluation of temporal kinetics of insulin and zinc release into the venous effluent from the pancreas. Both techniques were safe, reproducible, and easy to perform. The mean time required to obtain desired catheter position for venous sampling was 15 minutes shorter using the transsplenic technique. A clear biphasic insulin release profile was observed in both techniques. Statistically higher insulin concentration but similar zinc release after a glucose challenge was observed from splenic vein samples, as compared to the ones from the portal vein. To our knowledge, this is the first report of percutaneous methods to assess zinc release kinetics from the porcine pancreas.
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Anil Kumar
AK
Pillai
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
William
W
Silvers
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Preston
P
Christensen
Animal Resources Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Matthew
M
Riegel
Departments of Internal Medicine and Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Beverley
B
Adams-Huet
Departments of Internal Medicine and Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Ildiko
I
Lingvay
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA ; Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Xiankai
X
Sun
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Orhan K
OK
Öz
D000792:Angiography; D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D005471:Fluoroscopy / Q000379:methods; D005951:Glucose Tolerance Test / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007328:Insulin / Q000097:blood*; D050417:Insulin-Secreting Cells / Q000378:metabolism; D009339:Needles; D010179:Pancreas / Q000378:metabolism*; D011169:Portal Vein / Q000000981:diagnostic imaging / Q000473:pathology; D013154:Spleen / Q000098:blood supply / Q000000981:diagnostic imaging; D013552:Swine; D014057:Tomography, X-Ray Computed; D014463:Ultrasonography; D015032:Zinc / Q000097:blood* / Q000737:chemistry
D003287:Contrast Media; D007328:Insulin; D015032:Zinc