In vitro and in vivo biocompatibility, bioavailability and tolerance of an injectable vehicle for adipose-derived stem/stromal cells for plastic surgery indications.
Soft tissue reconstruction is a challenge in plastic surgery, when replacing lost materials and correcting contour defects. Many permanent and temporary fillers have been used to restore the volume of these lesions, but often with poor results and even complications. Adipose-derived stem/stromal cells (ASCs) and adipose tissue engineering have been suggested as valuable alternatives. In order to inject these cultured cells, it was essential to find a suitable vehicle. The purpose of this study was to evaluate Cytocare(®), an injectable medical device, composed of hyaluronic acid plus amino acids, vitamins and mineral salts. First, ASC viability and bioavailability in the 3 different available Cytocare(®) formulations using the MTT test were assessed; then an animal experiment, testing the tolerance after intradermal injections of both Cytocare(®) alone and with ASCs was carried out. Our in vitro results demonstrate a high biocompatibility of Cytocare(®) resulting in a better viability of ASCs when cultured in Cytocare(®) compared to culture medium (p < 0.05, Mann and Whitney). Cytocare(®) also permits their bioavailability and proliferation, making it a potential transfer vehicle that can retain the cells before their integration around the recipient site. Finally, our animal experiment shows that the ASC + Cytocare(®) combination is well tolerated. In conclusion, Cytocare(®) can be used as a biocompatible scaffold for cultured ASCs in therapeutic treatments, ensuring ASC bioavailability, as well as evidence of excellent tolerance in nude mice.
Cell and Tissue Bank, Edouard Herriot Hospital, 5 Place d'Arsonval, 69437 Lyon, Cedex 03, France; Institut de Biologie et Chimie des Protéines, LBTI, UMR 5305, CNRS/Université de Lyon, Lyon, France.
Charlotte
C
Lequeux
Cell and Tissue Bank, Edouard Herriot Hospital, 5 Place d'Arsonval, 69437 Lyon, Cedex 03, France; Institut de Biologie et Chimie des Protéines, LBTI, UMR 5305, CNRS/Université de Lyon, Lyon, France. Electronic address: jonathan.rodriguez@chu-lyon.fr.
Jonathan
J
Rodriguez
Department of Plastic Surgery, Croix Rousse Hospital, 103 Grande rue de la Croix Rousse, 69317 Lyon, Cedex 04, France.
Fabien
F
Boucher
Cell and Tissue Bank, Edouard Herriot Hospital, 5 Place d'Arsonval, 69437 Lyon, Cedex 03, France; Institut de Biologie et Chimie des Protéines, LBTI, UMR 5305, CNRS/Université de Lyon, Lyon, France.
Ondine
O
Rouyer
Cell and Tissue Bank, Edouard Herriot Hospital, 5 Place d'Arsonval, 69437 Lyon, Cedex 03, France; Institut de Biologie et Chimie des Protéines, LBTI, UMR 5305, CNRS/Université de Lyon, Lyon, France.
Odile
O
Damour
Department of Plastic Surgery, Croix Rousse Hospital, 103 Grande rue de la Croix Rousse, 69317 Lyon, Cedex 04, France.
Ali
A
Mojallal
Cell and Tissue Bank, Edouard Herriot Hospital, 5 Place d'Arsonval, 69437 Lyon, Cedex 03, France; Institut de Biologie et Chimie des Protéines, LBTI, UMR 5305, CNRS/Université de Lyon, Lyon, France.
Céline
C
Auxenfans
D017667:Adipocytes / Q000276:immunology / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology; D000818:Animals; D001682:Biological Availability; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D007108:Immune Tolerance; D008297:Male; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D019651:Plastic Surgery Procedures / Q000379:methods*; D033581:Stem Cell Transplantation / Q000379:methods*; D017154:Stromal Cells / Q000276:immunology / Q000637:transplantation*; D023822:Tissue Engineering / Q000379:methods*
Adipose tissue engineering; Adipose-derived stem/stromal cells; Plastic surgery; Vehicle