Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India.
Henckelia incana is an endemic medicinal plant used for the treatment of fever and skin allergy. In the present study shoot regeneration was evaluated on Murashige and Skoog's (MS) medium supplemented with auxins, Indole-3-acetic acid (IAA), Indole-3- butyric acid (IBA), 1-Naphthaleneacetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and cytokinins, 6-Benzylaminopurine (BAP) and Kinetin (Kn) at concentrations of 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mgl(-1). MS medium with IBA (18.08), NAA (17.83) and IAA (17.58) at 0.5 mgl(-1) concentrations showed efficient regeneration. Regenerated shoots were rooted on half-strength MS medium with and without 0.5 mgl(-1) IBA or NAA. The plantlets were successfully hardened in rooting trays (peat, vermiculite and sand) and transferred to field mileu. The genetic fidelity of in vitro raised plants was assessed by using three different single primer amplification reaction (SPAR) markers namely random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and direct amplification of mini-satellite DNA region (DAMD). The results consistently demonstrated true-to-true type propagation. This is the first report of in vitro propagation and establishment of true-to-true type genetic fidelity in H. incana.
Department of P G Studies and Research in Biotechnology, Government Science College, Bangalore, 560001 India.
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Prameela
Department of P G Studies and Research in Biotechnology, Government Science College, Bangalore, 560001 India.
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Ramakrishnaiah
P G Department of Studies and Research in Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Shankaraghatta, 577451 Karnataka India.
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V
Krishna
Department of P G Studies and Research in Biotechnology, Government Science College, Bangalore, 560001 India.
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AP
Deepalakshmi
Department of P G Studies and Research in Biotechnology, Government Science College, Bangalore, 560001 India.
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Naveen Kumar
Department of P G Studies and Research in Biotechnology, Government Science College, Bangalore, 560001 India.
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RN
Radhika
Auxin; Genetic fidelity; Henckelia incana; SPAR