A Cardiolipin-Deficient Mutant of Rhodobacter sphaeroides Has an Altered Cell Shape and Is Impaired in Biofilm Formation.
Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, highly curved phospholipid cardiolipin (CL) is reduced by 90%--produces ellipsoid-shaped cells that are impaired in biofilm formation. Reducing the concentration of CL did not cause significant defects in R. sphaeroides cell growth, swimming motility, lipopolysaccharide and exopolysaccharide production, surface adhesion protein expression, and membrane permeability. Complementation of the CL-deficient mutant by ectopically expressing CL synthase restored cells to their rod shape and increased biofilm formation. Treating R. sphaeroides cells with a low concentration (10 μg/ml) of the small-molecule MreB inhibitor S-(3,4-dichlorobenzyl)isothiourea produced ellipsoid-shaped cells that had no obvious growth defect yet reduced R. sphaeroides biofilm formation. This study demonstrates that CL plays a role in R. sphaeroides cell shape determination, biofilm formation, and the ability of the bacterium to adapt to its environment.


OBJECTIVE
Membrane composition plays a fundamental role in the adaptation of many bacteria to environmental stress. In this study, we build a new connection between the anionic phospholipid cardiolipin (CL) and cellular adaptation in Rhodobacter sphaeroides. We demonstrate that CL plays a role in the regulation of R. sphaeroides morphology and is important for the ability of this bacterium to form biofilms. This study correlates CL concentration, cell shape, and biofilm formation and provides the first example of how membrane composition in bacteria alters cell morphology and influences adaptation. This study also provides insight into the potential of phospholipid biosynthesis as a target for new chemical strategies designed to alter or prevent biofilm formation.
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Ti-Yu
TY
Lin
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Thiago M A
TM
Santos
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA DOE Great Lakes Bioenergy Research Center, Madison, Wisconsin, USA.
Wayne S
WS
Kontur
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA DOE Great Lakes Bioenergy Research Center, Madison, Wisconsin, USA.
Timothy J
http://orcid.org/0000-0001-8738-2467
TJ
Donohue
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA weibel@biochem.wisc.edu.
Douglas B
http://orcid.org/0000-0001-7797-2017
DB
Weibel
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D002308:Cardiolipins / Q000378:metabolism*; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D009154:Mutation; D012242:Rhodobacter sphaeroides / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D017855:Transferases (Other Substituted Phosphate Groups) / Q000172:deficiency* / Q000235:genetics
D001426:Bacterial Proteins; D002308:Cardiolipins; D008565:Membrane Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase