Anti-tumor effect of LTA combined with 5-FU on H22 tumor bearing mice.
OBJECTIVE
To study the effect of lipoteichoic acid (LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its anti-tumor mechanism.


METHODS
A total of 40 SPF grade Kunming mice were selected to establish H22 liver cancer model, and then the mice were divided into 4 groups at random with ten mice in each group. Group A was given saline lavage treatment, Group B was treated with 5-FU by intraperitoneal injection, Group C was treated with LTA by lump body injection; Group D was treated with LTA by lump body injection and 5-FU by intraperitoneal injection. Two weeks after the treatment, the mice in each group were executed and the tumor tissue was stripping and weighted, and the tumor growth inhibition ratio was calculated. Then the tumor tissue was processed for conventional embedding, sectioned to observe the expression of caspase-3, EGFR, TGF-α by immunohistochemical staining method.


RESULTS
The tumor inhibitory rate o f Group D was significantly higher than Groups B and C (P < 0.05); B, the tumor inhibitory rate o f Group B had no statistical difference compared with Group C (P > 0.05). The IDO values of TGF-α, EGFR proteins in Groups B, C, D mice tumor tissue were significantly lower than that in group A (P < 0.05); while IDO value of caspase-3 in Groups B, C, D group mice tumor tissue was significantly higher than that in Group A (P < 0.05). The IDO value of TGF-α, EGFR in Group D mice tumor tissue were significantly lower than that in Groups B and C; While IDO value of aspase-3 in Group D was significantly higher than that in Groups B and C (P < 0.05).


CONCLUSIONS
LTA combined with 5-FU can effectively inhibit the tumorigenesis of H22 tumor bearing mice, increase the caspase-3 protein expression, inhibit TGF-α and EGFR protein expression, further promote tumor cell apoptosis and play a synergistic antitumor effect.
Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
Bin
B
Wang
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Chang-Jiang
CJ
Lei
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Rong
R
Wu
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Lei
L
Li
Department of Hepatobiliary Surgery, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China.
Chun-Mei
CM
Deng
Department of Hepatobiliary Surgery, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China.
Wen-Xia
WX
Chen
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Fu-Rong
FR
Hu
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Hao-Cheng
HC
Long
Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. Electronic address: taozezhang@hotmail.com.
Ze-Zhang
ZZ
Tao
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Cheng
C
Zeng
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Jian-Bin
JB
Huang
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
Chun-Zhou
CZ
Chen
Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
De-Fa
DF
Ren
5-FU; Caspase-3; EGFR; LTA; TGF-α; Tumor inhibition rate