Ambient air pollution, blood mitochondrial DNA copy number and telomere length in a panel of diabetes patients.
BACKGROUND
Several previous studies proposed a link between particulate matter (PM) pollution and mitochondrial DNA copy number (MtDNAcn) and telomere length (TL). However, this evidence is quite limited and inconsistent, especially on how the particle size affects the associations and on whether there exists such an association with gaseous pollutants.


OBJECTIVE
We aimed to investigate the short-term associations of size-fractionated PM and gaseous pollutants with blood MtDNAcn and TL.


METHODS
We conducted a longitudinal panel study involving 6 repeated measurements among 35 Type 2 diabetes patients in Shanghai, China from April to June 2013. We measured the real-time concentrations of size-fractionated PM (0.25-10 μm) and criteria gaseous pollutants. Blood MtDNAcn and TL were tested by a quantitative real-time PCR-based assay. Linear mixed-effect models were used to explore their short-term associations using multiple lag periods, after controlling for individual characteristics, time trends and weather conditions.


RESULTS
In general, there were inverse but statistically non-significant associations between all pollutants and MtDNAcn. Coarse PM appeared to be more closely linked with MtDNAcn than smaller PM. The associations between various air pollutants and TL were generally positive but very weak. There were no clear lag patterns for these associations. The associations between air pollutants and MtDNAcn and TL were strengthened but still not significant among those who did not take statins regularly.


CONCLUSIONS
This study did not support short-term associations of PM or gaseous pollutants with blood MtDNAcn and TL in type 2 diabetes patients.
a School of Public Health, Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University , Shanghai , People's Republic of China .|b Key Laboratory of Health Technology Assessment of the Ministry of Health, Fudan University , Shanghai , People's Republic of China .
Yongjie
Y
Xia
a School of Public Health, Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University , Shanghai , People's Republic of China .|b Key Laboratory of Health Technology Assessment of the Ministry of Health, Fudan University , Shanghai , People's Republic of China .|c Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Fudan University , Shanghai , People's Republic of China , and.
Renjie
R
Chen
a School of Public Health, Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University , Shanghai , People's Republic of China .|b Key Laboratory of Health Technology Assessment of the Ministry of Health, Fudan University , Shanghai , People's Republic of China .
Cuicui
C
Wang
a School of Public Health, Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University , Shanghai , People's Republic of China .|b Key Laboratory of Health Technology Assessment of the Ministry of Health, Fudan University , Shanghai , People's Republic of China .
Jing
J
Cai
d Key Laboratory of Contemporary Anthropology of the Ministry of Education, Fudan University , Shanghai , People's Republic of China.
Lianghui
L
Wang
a School of Public Health, Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University , Shanghai , People's Republic of China .|b Key Laboratory of Health Technology Assessment of the Ministry of Health, Fudan University , Shanghai , People's Republic of China .
Zhuohui
Z
Zhao
d Key Laboratory of Contemporary Anthropology of the Ministry of Education, Fudan University , Shanghai , People's Republic of China.
Ji
J
Qian
a School of Public Health, Key Laboratory of Public Health Safety of the Ministry of Education, Fudan University , Shanghai , People's Republic of China .|b Key Laboratory of Health Technology Assessment of the Ministry of Health, Fudan University , Shanghai , People's Republic of China .|c Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Fudan University , Shanghai , People's Republic of China , and.
Haidong
H
Kan
D000368:Aged; D000393:Air Pollutants / Q000633:toxicity; D000397:Air Pollution / Q000009:adverse effects*; D056915:DNA Copy Number Variations / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D052638:Particulate Matter / Q000633:toxicity; D016615:Telomere
D000393:Air Pollutants; D004272:DNA, Mitochondrial; D052638:Particulate Matter
Air pollution; mitochondrial DNA copy number; panel study; telomere length