Circadian Gene Clock Regulates Psoriasis-Like Skin Inflammation in Mice.
There are several reports suggesting that the pathophysiology of psoriasis may be associated with aberrant circadian rhythms. However, the mechanistic link between psoriasis and the circadian time-keeping system, "the circadian clock," remains unclear. This study determined whether the core circadian gene, Clock, had a regulatory role in the development of psoriasis. For this purpose, we compared the development of psoriasis-like skin inflammation induced by the Toll-like receptor 7 ligand imiquimod (IMQ) between wild-type mice and mice with a loss-of-function mutation of Clock. We also compared the development of IMQ-induced dermatitis between wild-type mice and mice with a loss-of-function mutation of Period2 (Per2), another key circadian gene that inhibits CLOCK activity. We found that Clock mutation ameliorated IMQ-induced dermatitis, whereas the Per2 mutation exaggerated IMQ-induced dermatitis, when compared with wild-type mice associated with decreased or increased IL-23 receptor (IL-23R) expression in γ/δ+ T cells, respectively. In addition, CLOCK directly bound to the promoter of IL-23R in γ/δ+ T cells, and IL-23R expression in the mouse skin was under circadian control. These findings suggest that Clock is a novel regulator of psoriasis-like skin inflammation in mice via direct modulation of IL-23R expression in γ/δ+ T cells, establishing a mechanistic link between psoriasis and the circadian clock.
Department of Dermatology, University of Yamanashi, Faculty of Medicine, Yamanashi, Chuo, Japan.
Noriko
N
Ando
Department of Immunology, University of Yamanashi, Faculty of Medicine, Yamanashi, Chuo, Japan.
Yuki
Y
Nakamura
Department of Dermatology, University of Yamanashi, Faculty of Medicine, Yamanashi, Chuo, Japan.
Rui
R
Aoki
Department of Immunology, University of Yamanashi, Faculty of Medicine, Yamanashi, Chuo, Japan.
Kayoko
K
Ishimaru
Atopy Research Center, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
Hideoki
H
Ogawa
Atopy Research Center, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
Ko
K
Okumura
Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan.
Shigenobu
S
Shibata
Department of Dermatology, University of Yamanashi, Faculty of Medicine, Yamanashi, Chuo, Japan.
Shinji
S
Shimada
Department of Immunology, University of Yamanashi, Faculty of Medicine, Yamanashi, Chuo, Japan; Atopy Research Center, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan. Electronic address: anakao@yamanashi.ac.jp.
Atsuhito
A
Nakao
D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics* / Q000502:physiology; D002940:Circadian Rhythm; D003872:Dermatitis / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D005260:Female; D000077271:Imiquimod; D053759:Interleukin-23 / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D056950:Period Circadian Proteins / Q000235:genetics; D011565:Psoriasis / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology
D000634:Aminoquinolines; D053759:Interleukin-23; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D056926:CLOCK Proteins; C538944:Clock protein, mouse; D000077271:Imiquimod