Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta.
We investigated the effect of Lipofundin MCT/LCT and Intralipid on acetylcholine-induced nitric oxide- (NO-) mediated relaxation in rat aorta to determine which lipid emulsion (LE) is more potent in terms of inhibition of NO-induced relaxation. Dose-response curves of responses induced by acetylcholine, the calcium ionophore A23187, and sodium nitroprusside were generated using isolated rat aorta with or without LE. The effect of Lipofundin MCT/LCT on acetylcholine-induced endothelial nitric oxide synthase (eNOS) phosphorylation in human umbilical vein endothelial cells (HUVECs) was investigated using western blotting. Lipofundin MCT/LCT (0.1 and 0.2%) attenuated acetylcholine-induced relaxation in endothelium-intact aorta with or without tiron, whereas 0.2% Intralipid only inhibited relaxation. Lipofundin MCT/LCT inhibited relaxation induced by the calcium ionophore A23187 and sodium nitroprusside in endothelium-intact aorta, but Lipofundin MCT/LCT had no effect on sodium nitroprusside-induced relaxation in the endothelium-denuded aorta. Combined pretreatment with l-arginine plus Lipofundin MCT/LCT increased acetylcholine-induced maximal relaxation in endothelium-intact aorta compared with Lipofundin MCT/LCT alone. L-Arginine attenuated Lipofundin MCT/LCT-mediated inhibition of acetylcholine-induced eNOS phosphorylation in HUVECs. Taken together, Lipofundin MCT/LCT attenuated acetylcholine-induced NO-mediated relaxation via an inhibitory effect on the endothelium including eNOS, which is proximal to activation of guanylyl cyclase.
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea.
Seong-Ho
SH
Ok
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea.
Soo Hee
SH
Lee
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Jongsun
J
Yu
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Jungchul
J
Park
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea.
Il-Woo
IW
Shin
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Youngju
Y
Lee
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Hyunhoo
H
Cho
Department of Oral and Maxillofacial Surgery, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Mun-Jeoung
MJ
Choi
Department of Anesthesiology and Pain Medicine, Pusan National University Hospital, Biomed Research Institute, Pusan National University School of Medicine, Pusan 602-739, Republic of Korea.
Jiseok
J
Baik
Department of Anesthesiology and Pain Medicine, Pusan National University Hospital, Biomed Research Institute, Pusan National University School of Medicine, Pusan 602-739, Republic of Korea.
Jeong-Min
JM
Hong
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Jeong Yeol
JY
Han
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea.
Heon Keun
HK
Lee
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea.
Young-Kyun
YK
Chung
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea ; Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-715, Republic of Korea.
Ju-Tae
JT
Sohn
D000109:Acetylcholine / Q000008:administration & dosage*; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology*; D004338:Drug Combinations; D004347:Drug Interactions; D004655:Emulsions / Q000008:administration & dosage; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage*; D066298:In Vitro Techniques; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010743:Phospholipids / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D013012:Sorbitol / Q000008:administration & dosage; D013024:Soybean Oil / Q000008:administration & dosage; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000008:administration & dosage
D004338:Drug Combinations; D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D010743:Phospholipids; D014665:Vasodilator Agents; C545823:soybean oil, phospholipid emulsion; C008288:Lipofundin; D009569:Nitric Oxide; D013012:Sorbitol; D013024:Soybean Oil; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D000109:Acetylcholine