The expression profile of aquaporin 1 in rat myocardium after severe burns.
OBJECTIVE
To investigate the myocardial expression profile of aquaporin 1 (AQP1) in rats after severe burns.


METHODS
Ninety healthy male adult Wistar rats were randomly assigned to the following treatments: sham operation (control group, n = 6), immediate fluid resuscitation treatment post scalding (IF group, n = 42), and delayed fluid resuscitation treatment after scalding (DF group, n = 42). At 3, 6, 12, 24, 48, 72, and 120 h after scalding, myocardial water contents were assayed and AQP1 expressions were detected by real-time polymerase chain reaction and western blot. The AQP levels in the myocardium at 12 h after scalding were assayed by gene microarrays.


RESULTS
Scald injuries resulted in significantly synchronized increases in the myocardial water contents and the myocardial mRNA and protein expression of AQP1, with a peak at 12 h after scalding. Rats receiving delayed fluid resuscitation treatment had more severe myocardial edema and significantly higher myocardial AQP1 expressions than the rats receiving immediate fluid resuscitation treatment. The mRNAs of 6 other AQPs (AQP2, 3, 4, 6, 7, and 12b) were found to be changed in the myocardium among rats with different treatments.


CONCLUSIONS
AQP1 may play a functional role in the development of myocardial edema after scalding. Targeting AQP1 may provide opportunities for therapeutic intervention in myocardial edema following severe burns.
Wanli
W
Li
Longying
L
Zha
Zhiqing
Z
Li
Yuesheng
Y
Huang
Yiping
Y
Zhou
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D002056:Burns / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D005440:Fluid Therapy; D008297:Male; D046228:Microarray Analysis; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
D051398:Aquaporin 1