Proteomic analysis of Vibrio metschnikovii under cold stress using a quadrupole Orbitrap mass spectrometer.
Vibrio metschnikovii is a food-borne pathogen found in seafood worldwide. We studied the global proteome responses of V. metschnikovii under cold stress by nano-flow ultra-high-performance liquid chromatography coupled to a quadrupole Orbitrap mass spectrometer. A total of 2066 proteins were identified, among which 288 were significantly upregulated and 572 were downregulated. Functional categorization of these proteins revealed distinct differences between cold-stressed and control cells. Quantitative reverse transcription polymerase chain reaction analysis was also performed to determine the mRNA expression levels of seventeen cold stress-related genes. The results of this study should improve our understanding of the metabolic activities of cold-adapted bacteria and will facilitate a better systems-based understanding of V. metschnikovii.
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Juntao
J
Jia
Research Institute for Food Safety, Chinese Academy of Inspection and Quarantine, No. A3, Road Gaobeidian, 100123 Beijing, China. Electronic address: yingchencaiq@163.com.
Ying
Y
Chen
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Yinghui
Y
Jiang
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Zhengyi
Z
Li
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Liqing
L
Zhao
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Jian
J
Zhang
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Jing
J
Tang
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Liping
L
Feng
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Chengzhu
C
Liang
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Biao
B
Xu
Demo Center of Thermo Fisher Scientific Inc., 201206 Shanghai, China.
Peiming
P
Gu
Technological Center, Shandong Entry-Exit Inspection and Quarantine Bureau, 266002 Qingdao, China.
Xiwen
X
Ye
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D040901:Proteomics / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins
Cold stress; Proteomics; Vibrio metschnikovii