Sensitive Detection of ssDNA Using an LRET-Based Upconverting Nanohybrid Material.
Water-dispersible, optical hybrid nanoparticles are preferred materials for DNA biosensing due to their biocompatibility. Upconverting nanoparticles are highly desirable optical probes in sensors and bioimaging owing to their sharp emission intensity in the visible region. We herein report a highly sensitive ss-DNA detection based on an energy transfer system that uses a nanohybrid material synthesized by doping NaYF4:Tm(3+)/Yb(3+) upconverting nanoparticles (UCNPs) on silica coated polystyrene-co-acrylic acid (PSA) nanoparticles (PSA/SiO2) as the donor, and gold nanoparticles (AuNPs) decorated with Ir(III) complex as the acceptor. UCNPs tagged on PSA/SiO2 and the cyclometalated Ir(III)/AuNP conjugates were then linked through the ss-DNA sequence. Sequential addition of the target DNA to the probe molecular beacon complex resulted in the separation of the optical nanohybrid material and the quencher, leading to a measurable increase in the blue fluorescence emission intensity. Our results have shown a linear relationship between the fluorescence intensity and target DNA concentration down to the picomolar.
Biosensors, Bio-MEMS, Bionanotechnology Laboratory (BBBL), Institute for Global Food Security, School of Biological Sciences, Queen's University, Queen's University Belfast , 18-30 Malone Road, Belfast, BT9 5BN, United Kingdom.|Advanced Materials Laboratory, Institut National de la Recherche Scientifique - Énergie, Matériaux et Télécommunications, Université du Québec , Varennes, J3X 1S2 Québec, Canada.
Joe Gerald
JG
Jesu Raj
Advanced Materials Laboratory, Institut National de la Recherche Scientifique - Énergie, Matériaux et Télécommunications, Université du Québec , Varennes, J3X 1S2 Québec, Canada.
Marta
M
Quintanilla
Qatar Environment and Energy Research Institute, Qatar Foundation , Doha, Qatar.
Khaled A
KA
Mahmoud
Biosensors, Bio-MEMS, Bionanotechnology Laboratory (BBBL), Institute for Global Food Security, School of Biological Sciences, Queen's University, Queen's University Belfast , 18-30 Malone Road, Belfast, BT9 5BN, United Kingdom.
Andy
A
Ng
Advanced Materials Laboratory, Institut National de la Recherche Scientifique - Énergie, Matériaux et Télécommunications, Université du Québec , Varennes, J3X 1S2 Québec, Canada.|Centre for Self-Assembled Chemical Structures, McGill University , 845 Sherbrooke Street West, Montreal H3A 0G4 Québec, Canada.
Fiorenzo
F
Vetrone
Biosensors, Bio-MEMS, Bionanotechnology Laboratory (BBBL), Institute for Global Food Security, School of Biological Sciences, Queen's University, Queen's University Belfast , 18-30 Malone Road, Belfast, BT9 5BN, United Kingdom.|Department of Chemistry, Alfaisal University , Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh 11533, Saudi Arabia.
Mohammed
M
Zourob
D000179:Acrylates / Q000737:chemistry; D015374:Biosensing Techniques; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer; D005459:Fluorides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007495:Iridium / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D015018:Ytterbium / Q000737:chemistry; D015019:Yttrium / Q000737:chemistry
D000179:Acrylates; D056831:Coordination Complexes; D004277:DNA, Single-Stranded; D011137:Polystyrenes; C509855:poly(styrene-b-acrylic acid); C525362:sodium yttriumtetrafluoride; D007495:Iridium; D015019:Yttrium; D006046:Gold; D012822:Silicon Dioxide; D015018:Ytterbium; D005459:Fluorides
biosensing; energy transfer; luminescence; nanohybrid; upconversion