Expanding the ubiquitin code through post-translational modification.
Ubiquitylation is among the most prevalent post-translational modifications (PTMs) and regulates numerous cellular functions. Interestingly, ubiquitin (Ub) can be itself modified by other PTMs, including acetylation and phosphorylation. Acetylation of Ub on K6 and K48 represses the formation and elongation of Ub chains. Phosphorylation of Ub happens on multiple sites, S57 and S65 being the most frequently modified in yeast and mammalian cells, respectively. In mammals, the PINK1 kinase activates ubiquitin ligase Parkin by phosphorylating S65 of Ub and of the Parkin Ubl domain, which in turn promotes the amplification of autophagy signals necessary for the removal of damaged mitochondria. Similarly, TBK1 phosphorylates the autophagy receptors OPTN and p62 to initiate feedback and feedforward programs for Ub-dependent removal of protein aggregates, mitochondria and pathogens (such as Salmonella and Mycobacterium tuberculosis). The impact of PINK1-mediated phosphorylation of Ub and TBK1-dependent phosphorylation of autophagy receptors (OPTN and p62) has been recently linked to the development of Parkinson's disease and amyotrophic lateral sclerosis, respectively. Hence, the post-translational modification of Ub and its receptors can efficiently expand the Ub code and modulate its functions in health and disease.
Institute of Biochemistry II Goethe University, Frankfurt am Main, Germany.
Lina
L
Herhaus
Institute of Biochemistry II Goethe University, Frankfurt am Main, Germany ivan.dikic@biochem2.de.
Ivan
I
Dikic
D000107:Acetylation; D000690:Amyotrophic Lateral Sclerosis / Q000209:etiology / Q000235:genetics; D000818:Animals; D001343:Autophagy / Q000235:genetics; D006801:Humans; D063306:Mitophagy / Q000235:genetics / Q000502:physiology; D010300:Parkinson Disease / Q000209:etiology / Q000235:genetics; D010766:Phosphorylation / Q000235:genetics; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D017434:Protein Structure, Tertiary; D025801:Ubiquitin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D054875:Ubiquitination / Q000235:genetics*; D015003:Yeasts / Q000235:genetics / Q000502:physiology
D025801:Ubiquitin; D011494:Protein Kinases
mitophagy; phosphorylation; post‐translational modification; ubiquitin