A7RC peptide modified paclitaxel liposomes dually target breast cancer.
A7R peptide (ATWLPPR), a ligand of the NRP-1 receptor, regulates the intracellular signal transduction related to tumor vascularization and tumor growth. Here, we designed A7R-cysteine peptide (A7RC) surface modified paclitaxel liposomes (A7RC-LIPs) to achieve targeting delivery and inhibition of tumor growth and angiogenesis simultaneously. The cytotoxicity, inhibiting angiogenesis, and internalization of various liposomes by cells were assessed in vitro to confirm the influence of the peptide modification. The accumulations of A7RC-LIPs in various xenografts in mice were tracked to further identify the function of the peptide on the liposomes' surface. The results confirmed that A7RC peptides could enhance the uptake of vesicles by MDA-MB-231 cells, leading to stronger cytotoxicity in vitro and higher accumulation of vesicles in MDA-MB-231 xenografts in vivo. In addition, A7RC peptides enhanced the inhibitory effects of LIPs on the HUVEC tubular formation on Matrigel. The A7RC-LIPs may be promising drug carriers for anticancer therapy.
Department of Medical Oncology, The Tumor Hospital of Harbin Medical University, 150 Hapin Road, Harbin, 150086, China.
Jingyan
J
Cao
Ran
R
Wang
Ning
N
Gao
Minghui
M
Li
Xuyu
X
Tian
Weili
W
Yang
Ying
Y
Ruan
Chunlan
C
Zhou
Guangtian
G
Wang
Xiaoying
X
Liu
Shukun
S
Tang
Yan
Y
Yu
Ying
Y
Liu
Guangyu
G
Sun
Haisheng
H
Peng
Qun
Q
Wang
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004338:Drug Combinations; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology*; D011509:Proteoglycans / Q000737:chemistry* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
C000599882:Ala-Thr-Trp-Leu-Pro-Pro-Arg; D000970:Antineoplastic Agents; D004338:Drug Combinations; D007797:Laminin; D008024:Ligands; D008081:Liposomes; D009842:Oligopeptides; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D017239:Paclitaxel