Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium.
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.
Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy. ; Università degli Studi di Messina, School in Biology and Cellular Biotechnology, Faculty of Sciences, University of Messina, Messina, Italy, School in "Biology and Cellular Biotechnology", Faculty of Sciences, University of Messina, Messina, Italy.
Santina
S
Santisi
Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy.
Simone
S
Cappello
Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy.
Maurizio
M
Catalfamo
Università degli Studi di Catania, Department of Industrial Engineering, University of Catania, Catania, Italy, Department of Industrial Engineering, University of Catania, Catania, Italy.
Giuseppe
G
Mancini
Shahid Bahonar University of Kerman, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Mehdi
M
Hassanshahian
Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy.
Lucrezia
L
Genovese
Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy.
Laura
L
Giuliano
Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy.
Michail M
MM
Yakimov
D053521:Alcanivoraceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D059013:Microbial Consortia; D008969:Molecular Sequence Data; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D044223:Pseudomonas stutzeri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010578:Petroleum; D012336:RNA, Ribosomal, 16S
Alcanivorax; Pseudomonas; Rhodococcus; bioaugmentation; bioremediation