Circulating miRNAs revealed as surrogate molecular signatures for the early detection of breast cancer.
The miRNAs have well studied roles in cancer. Here, we identified altered miRNA expression by global miRNA profiling in peripheral blood mononuclear cells (PBMCs) of breast cancer (n = 15) and healthy subjects (n = 15), and further validated the selected miRNAs in PBMCs (n = 45), blood plasma (n = 45) and breast tissue samples (n = 09). The expression of altered miRNAs was also evaluated in PBMCs among early stage (n = 32), advanced stage (n = 13), triple positive (n = 5) and triple negative (n = 5) breast cancer patients. Results showed differential pattern of expressions of these miRNAs in multiple cohorts, however in early stage breast cancer, miR-106a-5p and miR-454-3p were upregulated (p < 0.05), miR-195-5p and miR-495 were downregulated (p < 0.05) in PBMCs. In addition, these miRNAs were also significantly associated with cancer and ErbB signaling pathways. Multiple regression analysis and receiver-operative curve (ROC) analysis of miR-195-5p and miR-495 with area under curve (AUC) of 0.901 showed best discriminating combination for early stage breast cancer detection. In summary, the present study delineated the importance of miR-195-5p and miR-495 miRNAs as prospective circulating surrogate molecular signatures for early detection of breast cancer.
Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India; Proteomics and Environmental Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India.
Sanjay
S
Mishra
Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India.
Amit Kumar
AK
Srivastava
Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India; Proteomics and Environmental Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India.
Shankar
S
Suman
Department of Surgical Oncology, King George's Medical University, Chowk, Lucknow, U.P., India.
Vijay
V
Kumar
Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India; Proteomics and Environmental Carcinogenesis Laboratory, CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Post Box 80, Lucknow, U.P., India. Electronic address: yogeshwer_shukla@hotmail.com.
Yogeshwer
Y
Shukla
D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D012372:ROC Curve; D059467:Transcriptome
D014408:Biomarkers, Tumor; D035683:MicroRNAs
Breast cancer; Circulating miRNAs; Molecular signatures; PBMCs; qRT-PCR