Patatin-related phospholipase A, pPLAIIIα, modulates the longitudinal growth of vegetative tissues and seeds in rice.
Patatin-related phospholipase A (pPLA) hydrolyses glycerolipids to produce fatty acids and lysoglycerolipids. The Oryza sativa genome has 21 putative pPLAs that are grouped into five subfamilies. Overexpression of OspPLAIIIα resulted in a dwarf phenotype with decreased length of rice stems, roots, leaves, seeds, panicles, and seeds, whereas OspPLAIIIα-knockout plants had longer panicles and seeds. OspPLAIIIα-overexpressing plants were less sensitive than wild-type and knockout plants to gibberellin-promoted seedling elongation. OspPLAIIIα overexpression and knockout had an opposite effect on the expression of the growth repressor SLENDER1 in the gibberellin signalling process. OspPLAIIIα-overexpressing plants had decreased mechanical strength and cellulose content, but exhibited increases in the expression of several cellulose synthase genes. These results indicate that OspPLAIIIα plays a role in rice vegetative and reproductive growth and that the constitutive, high activity of OspPLAIIIα suppresses cell elongation. The decreased gibberellin response in overexpressing plants is probably a result of the decreased ability to make cellulose for anisotropic cell expansion.
National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Guangmeng
G
Liu
National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Ke
K
Zhang
National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Jun
J
Ai
National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Xianjun
X
Deng
National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China hongyy@mail.hzau.edu.cn wangxue@umsl.edu.
Yueyun
Y
Hong
National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China Department of Biology, University of Missouri, St. Louis, MO 63121, USA Donald Danforth Plant Science Center, St. Louis, MO 63132, USA hongyy@mail.hzau.edu.cn wangxue@umsl.edu.
Xuemin
http://orcid.org/0000-0002-6251-6745
X
Wang
D000595:Amino Acid Sequence; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D010741:Phospholipases A / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D010741:Phospholipases A
Cellulose; dwarf; gibberellin; longitudinal growth; phospholipase A; phospholipids; rice seeds.