Measurement of lipid accumulation in Chlorella vulgaris via flow cytometry and liquid-state ¹H NMR spectroscopy for development of an NMR-traceable flow cytometry protocol.
In this study, we cultured Chlorella vulgaris cells with a range of lipid contents, induced via nitrogen starvation, and characterized them via flow cytometry, with BODIPY 505/515 as a fluorescent lipid label, and liquid-state 1H NMR spectroscopy. In doing so, we demonstrate the utility of calibrating flow cytometric measurements of algal lipid content using triacylglyceride (TAG, also known as triacylglycerol or triglyceride) content per cell as measured via quantitative 1H NMR. Ensemble-averaged fluorescence of BODIPY-labeled cells was highly correlated with average TAG content per cell measured by bulk NMR, with a linear regression yielding a linear fit with r2 = 0.9974. This correlation compares favorably to previous calibrations of flow cytometry protocols to lipid content measured via extraction, and calibration by NMR avoids the time and complexity that is generally required for lipid quantitation via extraction. Flow cytometry calibrated to a direct measurement of TAG content can be used to investigate the distribution of lipid contents for cells within a culture. Our flow cytometry measurements showed that Chlorella vulgaris cells subjected to nitrogen limitation exhibited higher mean lipid content but a wider distribution of lipid content that overlapped the relatively narrow distribution of lipid content for replete cells, suggesting that nitrogen limitation induces lipid accumulation in only a subset of cells. Calibration of flow cytometry protocols using direct in situ measurement of TAG content via NMR will facilitate rapid development of more precise flow cytometry protocols, enabling investigation of algal lipid accumulation for development of more productive algal biofuel feedstocks and cultivation protocols.
Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, United States of America.
Michael S
MS
Bono
Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, United States of America.
Ravi D
RD
Garcia
Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, United States of America.
Dylan V
DV
Sri-Jayantha
Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, United States of America.
Beth A
BA
Ahner
Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, United States of America; Division of Hematology and Medical Oncology, Weill Cornell Medical College, New York, NY 10065, United States of America.
Brian J
BJ
Kirby
D056804:Biofuels; D018533:Biomass; D048408:Chlorella vulgaris / Q000737:chemistry / Q000378:metabolism*; D005434:Flow Cytometry; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism
D056804:Biofuels; D008055:Lipids; D014280:Triglycerides; D009584:Nitrogen