Effect of non-steroidal anti-inflammatory drugs on the increasing the incidence of colonic anastomosis in rats.
BACKGROUND
Anastomotic leakage is one of serious complications of colorectal surgery. Research is inconsistent about whether non-steroidal anti-inflammatory drugs influence the healing of colorectal anastomoses and increase the incidence of anastomotic leakage.


OBJECTIVE
To study the influence of NSAIDs on the healing of rat colonic anastomoses.


METHODS
This was an animal randomized-control trial. This study was approved by the ethical committee of Yangpu Hospital, Tongji University.


METHODS
90 healthy Sprague-Dawley rats were randomly divided into 6 groups of 15 rats/group. Trail was performed in C (cotrol group) with no drugs, group M with morphine for analgesia, group F with flurbiprofen axeil, group L with lornoxicam, and group P with parecoxib sodium.


METHODS
The main outcomes measures were serological indexes including vascular endothelial growth factor, prostaglandin E2, hydroxyproline, and C reactive protein; histological specimens from the anastomotic stoma tissue including the collagen proportion, and hydroxyproline, cycloxygenase-2, and vascular endothelial growth factor content; physical indicators, including stoma fracture pressure, fracture strength and anastomotic leakage.


RESULTS
No significant difference was observed among the indices of each group (P > 0.05). A significant difference occurred after operation (P < 0.05), with the data for groups K and M being dramatically higher than those for group F.


CONCLUSIONS
The study was nonblinded.


CONCLUSIONS
The postoperative usages of non-steroidal anti-inflammatory drugs can decrease the strength of anastomotic tissue, and increase the incidence of anastomotic leakage.
Department of Anesthesiology, Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
Chengdong
C
Ji
Department of Anesthesiology, Changhai Hospital, Second Military Medical University Shanghai 200433, China.
Yuanchang
Y
Xiong
Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
Xin
X
Pan
Department of Anesthesiology, Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
Xuan
X
Guo
Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
Zhen
Z
Li
Department of Anesthesiology, Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
Shuwen
S
Qian
Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
Chang
C
Xu
Yangpu Hospital, School of Medicine, Tongji University Shanghai 200090, China.
De-Hua
DH
Yu
Changzheng Hospital, Second Military Medical University Shanghai 200433, China.
Wan-Qing
WQ
Liao
D000714:Anastomosis, Surgical / Q000009:adverse effects; D057868:Anastomotic Leak / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000097:blood; D006909:Hydroxyproline / Q000097:blood; D008297:Male; D023421:Models, Animal; D011312:Pressure; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D013997:Time Factors; D000267:Tissue Adhesions; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D003094:Collagen; D002097:C-Reactive Protein; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D015232:Dinoprostone; D006909:Hydroxyproline
Non-steroidal anti-inflammatory drugs (NSAIDs); anastomotic leakage; colonic anastomotic stoma