Theoretical investigation on the performance of DNA electrophoresis under programmed step electric field strength: Two-step condition.
Programmed step electric field strength is a simple-to-use technique that has already been reported to be effective to enhance the efficiency or speed of DNA electrophoresis. However, a global understanding and the details of this technique are still vague. In this paper, we investigated the influence of programmed step electric field strength by theoretical calculation and concentrated on a basic format named as two-step electric field strength. Both subtypes of two-step electric field strength conditions were considered. The important parameters, such as peak spacing, peak width, resolution, and migration time, were calculated in theory to understand the performance of DNA electrophoresis under programmed step electric field strength. The influence of two-step electric field strength on DNA electrophoresis was clearly revealed on a diagram of resolution versus migration time. Both resolution and speed of DNA electrophoresis under two-step electric field strength conditions are simply expressed by the shape of curves in the diagram. The possible shapes of curve were explored by calculation and shown in this paper. The subtype II of two-step electric field strength brings drastic variation on the resolution. Its limitations of enhancement and deterioration of resolution were predicted in theory.
Institute of Photonics and Bio-Medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, China.
Yi
Y
Ni
Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai, China.
Chenchen
C
Liu
Institute of Photonics and Bio-Medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, China.
Qinmiao
Q
Chen
School of Optoelectronic Engineering, Changzhou Institute of Technology, Changzhou, Jiangsu, China.
Xifang
X
Zhu
Institute of Photonics and Bio-Medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, China.|School of Optoelectronic Engineering, Changzhou Institute of Technology, Changzhou, Jiangsu, China.
Xiaoming
X
Dou
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004574:Electromagnetic Fields; D019075:Electrophoresis, Capillary
D004247:DNA
Capillary electrophoresis; DNA; Programmed step electric field strength; Two-step electric field strength