Anti-Inflammatory Activity of Heterocarpin from the Salt Marsh Plant Corydalis heterocarpa in LPS-Induced RAW 264.7 Macrophage Cells.
The inhibitory effect of three chromones 1-3 and two coumarins 4-5 on the production of nitric oxide (NO) was evaluated in LPS-induced RAW 264.7 macrophage cells. Among the compounds tested heterocarpin (1), a furochromone, significantly inhibited its production in a dose-dependent manner. In addition, heterocarpin suppressed prostaglandin E2 (PGE2) production and expression of cytokines such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
Division of Marine Bioscience, Korea Maritime & Ocean University, Busan 606-791, Korea. ahyou2@hanmail.net.
You Ah
YA
Kim
Department of Food and Nutrition, Silla University, Busan 617-736, Korea. cskong@silla.ac.kr.
Chang-Suk
CS
Kong
Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Korea. qkrgygus@empal.com.
Hyo Hyun
HH
Park
Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Korea. eklee@ynu.ac.kr.
Eunkyung
E
Lee
Food and Safety Research Center, National Fisheries Research & Development Institute, Busan 619-705, Korea. suni1@korea.kr.
Mi-Soon
MS
Jang
Food and Safety Research Center, National Fisheries Research & Development Institute, Busan 619-705, Korea. dennis011@korea.kr.
Ki-Ho
KH
Nam
Division of Marine Bioscience, Korea Maritime & Ocean University, Busan 606-791, Korea. ywseo@kmou.ac.kr.|Ocean Science & Technology School, Korea Maritime & Ocean University, Busan 606-791, Korea. ywseo@kmou.ac.kr.
Youngwan
Y
Seo
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002867:Chromones / Q000737:chemistry / Q000494:pharmacology*; D031311:Corydalis / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000067996:RAW 264.7 Cells; D015398:Signal Transduction / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002867:Chromones; D003374:Coumarins; D052246:Cyclooxygenase 2 Inhibitors; D016207:Cytokines; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D015232:Dinoprostone
Corydalis heterocarpa; anti-inflammatory effect; furochromone; heterocarpin