Possible involvement of TGF-β/periostin in fibrosis of right atrial appendages in patients with atrial fibrillation.
Atrial fibrosis contributes to development and recurrence of atrial fibrillation (AF). TGF-β and periostin have been reported to be involved in fibrogenesis. Here we investigated the role of TGF-β and periostin in atrial fibrosis of AF and in the recurrence of AF after surgery ablation. Western blot, Masson staining, immunohistochemistry and colorimetry were performed to detect the degree of atrial fibrosis and the expression of TGF-β, periostin and collagens in 70 biopsies of right atrial appendage (RAA) obtained in this study. Then the patients who received surgical ablation were followed up for about one year. The results showed an increasing gradient of atrial expression of TGF-β, periostin and collagens paralleled by a higher level of atrial fibrosis in control, SR and AF groups. The expression of TGF-β and periostin was significantly correlated with fibrotic markers. In addition, LAD and the expression of TGF-β were larger or higher in recurrence group than that in nonrecurrence group after surgery ablation. The results suggest that upregulated expression of TGF-β and periostin in RAAs is correlated with the degree of atrial fibrosis in patients with AF.
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Han
H
Wu
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Jun
J
Xie
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Guan-Nan
GN
Li
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Qin-Hua
QH
Chen
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Ran
R
Li
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Xin-Lin
XL
Zhang
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Li-Na
LN
Kang
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School Nanjing 210008, China.
Biao
B
Xu
D000328:Adult; D020517:Atrial Appendage / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D001281:Atrial Fibrillation / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D001706:Biopsy; D015153:Blotting, Western; D017115:Catheter Ablation; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003094:Collagen / Q000032:analysis; D005260:Female; D005355:Fibrosis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012008:Recurrence; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000032:analysis*; D016896:Treatment Outcome; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D016212:Transforming Growth Factor beta; D003094:Collagen
Periostin; atrial fibrillation; fibrosis; recurrence; transforming growth factor-β