Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification.
Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID50 per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Rie
R
Koide
Shoichi
S
Sakaguchi
Makoto
M
Ogawa
Takayuki
T
Miyazawa
D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000821:virology; D002415:Cats / Q000821:virology; D018103:Morbillivirus; D018185:Morbillivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity