Torque teno sus virus 1 (TTSuV1) and 2 (TTSuV2) viral loads in serum of postweaning multisystemic wasting syndrome (PMWS)-affected and healthy pigs in Brazil.
Associations between Torque teno sus viruses (TTSuVs) and the occurrence of postweaning multisystemic wasting syndrome (PMWS) have been reported with controversial results. Currently, no studies have been performed comparing simultaneously viral loads of TTSuVs and PCV2. To examine the role for TTSuVs in PMWS-affected animals, a SYBR Green-based quantitative PCR (qPCR) was designed to detect and quantify TTSuV1, TTSuV2 and PCV2 genomes in swine sera. TTSuV1 genome loads were significantly higher in healthy adults than in young and SPF animals (p<0.05) suggesting that the prevalence of TTSuV1 infection increases with age and bears no association with PMWS. Regarding TTSuV2, no significant variation was detected in viral loads within any of the groups. As expected, PCV2 genome loads were higher in PMWS-affected swine than in healthy or SPF animals (p<0.001). These findings provide clear evidence to indicate that neither TTSuV1 nor TTSuV2 viral loads have any correlation with the occurrence of PMWS.
Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS 92990-000, Brazil; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Sarmento Leite 500, sala 208, Porto Alegre, RS 90050-170, Brazil. Electronic address: thais.fumaco@gmail.com.
Thais Fumaco
TF
Teixeira
Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS 92990-000, Brazil; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Sarmento Leite 500, sala 208, Porto Alegre, RS 90050-170, Brazil.
Samuel Paulo
SP
Cibulski
Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS 92990-000, Brazil.
Helton Fernandes
HF
dos Santos
Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS 92990-000, Brazil.
Adriéli
A
Wendlant
Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Sarmento Leite 500, sala 208, Porto Alegre, RS 90050-170, Brazil.
Francisco Esmaile
FE
de Sales Lima
Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS 92990-000, Brazil.
Candice
C
Schmidt
Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Sarmento Leite 500, sala 208, Porto Alegre, RS 90050-170, Brazil.
Ana Cláudia
AC
Franco
Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS 92990-000, Brazil; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Sarmento Leite 500, sala 208, Porto Alegre, RS 90050-170, Brazil.
Paulo Michel
PM
Roehe
D000818:Animals; D052160:Benzothiazoles; D001938:Brazil; D017925:Circovirus / Q000235:genetics*; D003959:Diamines; D016679:Genome, Viral / Q000235:genetics*; D009930:Organic Chemicals; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000453:epidemiology* / Q000821:virology*; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012698:Serologic Tests / Q000662:veterinary; D044967:Serum / Q000821:virology*; D013552:Swine; D022783:Torque teno virus / Q000235:genetics*; D019562:Viral Load / Q000235:genetics / Q000662:veterinary*
D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I
Anellovirus; Postweaning multisystemic wasting syndrome (PMWS); Quantitative PCR (qPCR); Torque teno sus virus (TTSuV)