Evaluation of acute effects of melatonin on ethanol drinking in ethanol naïve rats.
OBJECTIVE
The objective was to evaluate the acute effect of melatonin on ethanol drinking in ethanol naïve rats and to determine the specificity of the effect of melatonin on ethanol intake as compared to an intake of plain tap water or sugar water.


METHODS
A total of three experiments (2 weeks duration each) using different drinking solutions (ethanol, plain tap water, sugar water) was conducted in individually housed male wistar rats of 5 weeks age. Each animal had access to bottles containing drinking solutions for 2 h a day. In each experiment, on day 1, day 2, day 4, day 5, day 8, day 9, day 11, day 12 rats received drinking solutions. Each individual rat received single doses of saline, melatonin (50 mg and 100 mg/kg), and naltrexone on day 2, 5, 9, and 12, 1-h before receiving drinking solution. The order of drug administration is permuted such a way that each animal received the drugs in a different order in different experiments.


RESULTS
Melatonin has significantly decreased ethanol consumption by the rats and effect is dose-dependent. Naltrexone also has caused a significant reduction in the ethanol consumption. The maximum reduction in ethanol consumption was seen with melatonin 100 mg/kg dose compared to melatonin 50 mg/kg and naltrexone. There was no statistically significant effect of melatonin on plain water and sugar solution intake.


CONCLUSIONS
Melatonin decreases ethanol consumption in ethanol naïve rats. The effect of melatonin is similar to naltrexone affecting selectively ethanol consumption, but not plain water and sugar water consumption.
Department of Pharmacology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
Zahoor Ahmad
ZA
Rather
Department of Pharmacology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
Mukta N
MN
Chowta
Department of Physiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
Ganaraja
G
Bolumbu
Department of Pharmacology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
K B
KB
Rakesh
D000428:Alcohol Drinking / Q000517:prevention & control*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D008297:Male; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D009271:Naltrexone / Q000494:pharmacology*; D009292:Narcotic Antagonists / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D009292:Narcotic Antagonists; D000431:Ethanol; D009271:Naltrexone; D008550:Melatonin
Dependence; ethanol; melatonin; naltrexone