Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation.
Pathological expansion of adipose tissue contributes to the metabolic syndrome. Distinct depots develop at various times under different physiological conditions. The transcriptional cascade mediating adipogenesis is established in vitro, and centres around a core program involving PPARγ and C/EBPα. We developed an inducible, adipocyte-specific knockout system to probe the requirement of key adipogenic transcription factors at various stages of adipogenesis in vivo. C/EBPα is essential for all white adipogenic conditions in the adult stage, such as adipose tissue regeneration, adipogenesis in muscle and unhealthy expansion of white adipose tissue during high-fat feeding or due to leptin deficiency. Surprisingly, terminal embryonic adipogenesis is fully C/EBPα independent, but does however depend on PPARγ; cold-induced beige adipogenesis is also C/EBPα independent. Moreover, C/EBPα is not vital for adipocyte survival in the adult stage. We reveal a surprising diversity of transcriptional signals required at different stages of adipogenesis in vivo.
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Qiong A
QA
Wang
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Caroline
C
Tao
Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Lei
L
Jiang
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Mengle
M
Shao
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Risheng
R
Ye
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.|LIFA Diabetes, Lilly Research Laboratories, Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
Yi
Y
Zhu
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Ruth
R
Gordillo
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Aktar
A
Ali
Center for Immunology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Yun
Y
Lian
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
William L
WL
Holland
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Rana K
RK
Gupta
Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.|Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Philipp E
PE
Scherer
D017667:Adipocytes / Q000502:physiology*; D050156:Adipogenesis; D052436:Adipose Tissue, White / Q000166:cytology; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D050260:Carbohydrate Metabolism; D048430:Cell Shape; D059305:Diet, High-Fat / Q000009:adverse effects; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D055786:Gene Knockout Techniques; D050356:Lipid Metabolism; D008297:Male; D008820:Mice, Obese; D008822:Mice, Transgenic; D009928:Organ Specificity; D047495:PPAR gamma / Q000378:metabolism; D014158:Transcription, Genetic
D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D047495:PPAR gamma