Genome-wide analysis of esterase-like genes in the striped rice stem borer, Chilo suppressalis.
The striped rice stem borer, Chilo suppressalis, a destructive pest of rice, has developed high levels of resistance to certain insecticides. Esterases are reported to be involved in insecticide resistance in several insects. Therefore, this study systematically analyzed esterase-like genes in C. suppressalis. Fifty-one esterase-like genes were identified in the draft genomic sequences of the species, and 20 cDNA sequences were derived which encoded full- or nearly full-length proteins. The putative esterase proteins derived from these full-length genes are overall highly diversified. However, key residues that are functionally important including the serine residue in the active site are conserved in 18 out of the 20 proteins. Phylogenetic analysis revealed that most of these genes have homologues in other lepidoptera insects. Genes CsuEst6, CsuEst10, CsuEst11, and CsuEst51 were induced by the insecticide triazophos, and genes CsuEst9, CsuEst11, CsuEst14, and CsuEst51 were induced by the insecticide chlorantraniliprole. Our results provide a foundation for future studies of insecticide resistance in C. suppressalis and for comparative research with esterase genes from other insect species.
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.|Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.
Baoju
B
Wang
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.|Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.
Ying
Y
Wang
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.|Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.
Yang
Y
Zhang
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.|Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.
Ping
P
Han
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.|Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.
Fei
F
Li
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.|Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing 210095, Jiangsu, China.
Zhaojun
Z
Han
D000595:Amino Acid Sequence; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D056726:Genetic Association Studies / Q000379:methods*; D007300:Insect Control; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000633:toxicity; D008969:Molecular Sequence Data; D063086:Organothiophosphates / Q000633:toxicity; D012275:Oryza / Q000235:genetics* / Q000469:parasitology; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D016415:Sequence Alignment; D014230:Triazoles / Q000633:toxicity; D062367:ortho-Aminobenzoates / Q000633:toxicity
D007306:Insecticides; D063086:Organothiophosphates; D010940:Plant Proteins; D014230:Triazoles; D062367:ortho-Aminobenzoates; C012584:triazophos; C517733:chlorantranilipole; D004950:Esterases
Chilo suppressalis; analyse phylogénétique; esterase gene; genome; gène d’estérase; génome; induction; phylogenetic analysis