The relation of the number of hydrogen-bond acceptors with recoveries of immunosuppressants in DBS analysis.
BACKGROUND
We investigated the influence of the number of hydrogen-bond acceptors on the recovery of immunosuppressant drugs and their structural analogs. This hypothesis was tested by evaluation of the extraction recoveries of tacrolimus, ascomycin, sirolimus, everolimus and temsirolimus with 12, 12, 13, 14 and 16 hydrogen-bond acceptors, respectively.


RESULTS
With an increasing number of hydrogen-bond acceptors of sirolimus, everolimus and temsirolimus, a decrease in recoveries was found while ascomycin showed recoveries corresponding to those of tacrolimus.


CONCLUSIONS
This study showed that the number of hydrogen-bond acceptors of the analyte of interest may influence the recoveries in dried blood spot analysis and is a relevant factor to be investigated during method development and validation.
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.
Remco A
RA
Koster
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.
Jan-Willem C
JW
Alffenaar
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.
Rixt
R
Botma
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.
Ben
B
Greijdanus
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.
Donald Ra
DR
Uges
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.|University of Groningen, Department of Pharmacy, Section Pharmacotherapy & Pharmaceutical Care, Groningen, The Netherlands.
Jos Gw
JG
Kosterink
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, Clinical Pharmaceutical & Toxicological Laboratory, PO Box 30.001, 9700 RB, Groningen, The Netherlands.|University of Groningen, Department of Pharmacy, Section Pharmacokinetics, Toxicology & Targeting, Groningen, The Netherlands.
Daan J
DJ
Touw
D059788:Dried Blood Spot Testing / Q000379:methods*; D006801:Humans; D006860:Hydrogen Bonding; D007166:Immunosuppressive Agents / Q000097:blood*
D007166:Immunosuppressive Agents