Magnetically Self-Assembled Colloidal Three-Dimensional Structures as Cell Growth Scaffold.
Understanding the chemical and physical conditions for cell growth is important from biological and medical aspects. Many tissues and cell types (e.g., epithelial cells and neurons) naturally grow on surfaces that span in three-dimensions and offer structural or mechanical support. The scaffold surface has to promote adhesion and cell proliferation as well as support their weight and retain its structural integrity. Here, we present a flexible method that uses self-assembly of micrometer superparamagnetic particles to produce appropriate scaffold surfaces with controllable general appearance in three dimensions, such as oriented membranes, branched structure, or void network. As a proof of principle, the Chinese hamster ovary epithelial cell line was successfully grown for several days on inclined membranes. Robustness of the oriented membrane architecture was probed with optical tweezers. We measured the magnetic force holding one particle in a self-assembled upright hexagonal sheet and modeled it as a sum of pair interaction forces between spatially arrested static dipoles.
Jožef Stefan Institute , Jamova cesta 39, SI-1000 Ljubljana, Slovenia.
Gašper
G
Kokot
Faculty of Medicine, Institute of Biophysics, University of Ljubljana , Vrazov trg 2, SI-1000 Ljubljana, Slovenia.
Špela
Š
Zemljič Jokhadar
Faculty of Medicine, Institute of Biophysics, University of Ljubljana , Vrazov trg 2, SI-1000 Ljubljana, Slovenia.
Urška
U
Batista
Faculty of Mathematics and Physics, University of Ljubljana , Jadranska 19, SI-1000 Ljubljana, Slovenia.
Dušan
D
Babič
D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation; D003102:Colloids / Q000138:chemical synthesis / Q000737:chemistry*; D003412:Cricetulus; D060526:Magnetic Fields; D015394:Molecular Structure; D054457:Tissue Scaffolds / Q000737:chemistry*
D003102:Colloids