Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection.
A tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) is a negative feedback regulator that controls excessive immune responses in vertebrates. The sequence of tick hemolymph TRAFD1 from the hard tick Haemaphysalis longicornis (HlTRAFD1) was analyzed after identification and cloning from the expressed sequence tag database. RT-PCR and Western blot analyses showed that HlTRAFD1 transcript and protein levels after blood feeding were present in all developmental stages, and the transcript level was consistently high in all organs examined from adult female ticks upon engorgement. Knockdown of HlTRAFD1 gene by RNA interference did not affect blood feeding or oviposition. However, HlTRAFD1 silencing affected the expression of the longicin gene, a defensin-like molecule, but not the lysozyme gene. Moreover, the survival rate of HlTRAFD1-silenced ticks was lower, and the number of E. coli was higher in the hemolymph and plasmatocytes after E. coli injection compared to the control group. These results suggested that HlTRAFD1 strongly affected both the humoral and cellular immunity of ticks.
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
Rie
R
Takechi
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
Remil Linggatong
RL
Galay
Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan; Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
Tomohide
T
Matsuo
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Hiroki
H
Maeda
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Kodai
K
Kusakisako
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Melbourne Rio
MR
Talactac
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Masami
M
Mochizuki
Zen-noh Institute of Animal Health, Ohja, Sakura, Chiba 285-0043, Japan.
Kozo
K
Fujisaki
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. Electronic address: tetsuya@ms.kagoshima-u.ac.jp.
Tetsuya
T
Tanaka
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D005247:Feeding Behavior; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism / Q000382:microbiology; D054884:Host-Pathogen Interactions; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D026863:Ixodidae / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D034622:RNA Interference; D011817:Rabbits; D012098:Reproduction; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
D047908:Intracellular Signaling Peptides and Proteins; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; C485184:enhanced green fluorescent protein; C096944:longicin; D049452:Green Fluorescent Proteins; D015107:4-Butyrolactone
Bacteria; Defensin; Hemocyte; Innate immunity; TRAFD1; Tick