Chemical Analysis in the Corpus Callosum Following Traumatic Axonal Injury using Fourier Transform Infrared Microspectroscopy: A Pilot Study.
Evaluating traumatic axonal injury remains challenging in clinical and forensic sciences as its identification is difficult using routine diagnostic methods. This study used Fourier transform infrared microspectroscopy to detect TAI within the corpus callosum in an animal model. Protein conformational analysis revealed significantly increased β-sheet and β-turn contents paralleled by a decrease in α-helix content at 24 h postinjury, while the antiparallel β-sheet content was decreased at 12 h postinjury. Compared with the control group, the lipid/protein ratio was significantly reduced in all of the injured groups. At 24 h postinjury, there were increases in the olefinic=CH and CH3 group of lipids accompanied by the decreased CH2 group, but the results at 12 and 72 h were contrary to that at 24 h. Our study showed that FTIRM could differentiate injured from normal white matter at different time points following TBI via examination of these infrared spectral parameters.
Department of Forensic Medicine, Xi'an Jiaotong University, 74 West Yanta Road, Xi'an, Shanxi, China.
Ji
J
Zhang
Department of Forensic Medicine, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
Liang
L
Liu
Department of Forensic Medicine, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
Jiao
J
Mu
Collaborative Innovation Center of Judicial Civilization, Key Laboratory of Evidence Science, China Univerisy of Political Science and Law, 116 Lugu Road, Beijing, China.
Tiantong
T
Yang
Department of Pathophysiology, Shenzhen Univeristy, 1688 Nanhai Road, Shenzhen, China.
Na
N
Zheng
Department of Forensic Medicine, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
Hongmei
H
Dong
D000818:Animals; D016022:Case-Control Studies; D003337:Corpus Callosum / Q000737:chemistry* / Q000293:injuries / Q000648:ultrastructure; D020833:Diffuse Axonal Injury / Q000473:pathology*; D005583:Fourier Analysis; D007150:Immunohistochemistry; D008055:Lipids / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D010865:Pilot Projects; D011487:Protein Conformation; D017207:Rats, Sprague-Dawley; D013057:Spectrum Analysis
D008055:Lipids
Fourier transform infrared microspectroscopy; animal study; forensic pathology; forensic science; traumatic axonal injury; traumatic brain injury