Gastroprotective effect of carob (Ceratonia siliqua L.) against ethanol-induced oxidative stress in rat.
BACKGROUND
We aimed in the present study, at investigating the gastroprotective effect of carob pods aqueous extract (CPAE) against ethanol-induced oxidative stress in rats as well as the mechanism implicated.


METHODS
Adult male wistar rats were used and divided into six groups of ten each: control, EtOH (80% v/v, 4 g/kg b.w.), EtOH 80% + various doses of CPAE (500, 1000 and 2000 mg/kg, b.w.) and EtOH + Famotidine (10 mg/kg, p.o.) Animals were perorally (p.o.) pre-treated with CPAE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) for two hours.


RESULTS
The colorimetric analysis demonstrated that the CPAE exhibited an importance in vitro antioxidant activity against ABTS and DPPH radicals. We found that CPAE pretreatment in vivo, protected against EtOH-induced macroscopic and histological changes induced in stomach mucosa. Carob extract administration also protected against alcohol-induced volume gastric juice decrease. More importantly, We showed that CPAE counteracted EtOH-induced gastric lipoperoxidation, reversed the decrease of sulfhydryl groups (-SH) an hydrogen peroxide (H2O2) levels, and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).


CONCLUSIONS
These findings suggest that CPAE exerted a potential gastro-protective effect against EtOH-induced oxidative stress in rats, due in part, to its antioxidants properties.
Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis. Campus Universitaire El Manar II, Tunis, 2092, Tunisia. rtibikais@yahoo.fr.|Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Resssources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P, 382-9000, Béja, Tunisia. rtibikais@yahoo.fr.
Kaïs
K
Rtibi
Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Resssources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P, 382-9000, Béja, Tunisia.|Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
Mohamed Amine
MA
Jabri
Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Resssources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P, 382-9000, Béja, Tunisia.
Slimen
S
Selmi
Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis. Campus Universitaire El Manar II, Tunis, 2092, Tunisia.
Abdelaziz
A
Souli
Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Resssources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P, 382-9000, Béja, Tunisia.|Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
Hichem
H
Sebai
INSERM, U1149, Centre de Recherche Sur l'Inflammation, Faculté de Médecine X. Bichat, 75018, Paris, France.
Jamel
J
El-Benna
Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis. Campus Universitaire El Manar II, Tunis, 2092, Tunisia.
Mohamed
M
Amri
Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Resssources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P, 382-9000, Béja, Tunisia.
Lamjed
L
Marzouki
D000818:Animals; D005685:Galactans / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D008351:Mannans / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D053149:Plant Gums / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects*
D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D020011:Protective Agents; C017471:locust bean gum