Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays.
Here we describe the procedure for expression and purification of recombinant ATE1 from E. coli. This method is easy and convenient and can result in one-step isolation of milligram amounts of soluble enzymatically active ATE1 at nearly 99 % purity. We also describe a procedure for expression and purification of E. coli Arg-tRNA synthetase essential for the arginylation assays described in the next two chapters.
Department of Animal Biology, University of Pennsylvania, Philadelphia, PA, USA.
Junling
J
Wang
Anna S
AS
Kashina
D019881:Aminoacyltransferases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D057075:Enzyme Assays; D015870:Gene Expression; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D001120:Arginine; D019881:Aminoacyltransferases; C019727:arginyltransferase