Two site genetic incorporation of varying length polyethylene glycol into the backbone of one peptide.
Polyethylene glycol (PEG) of different lengths was genetically incorporated into the backbone of a polypeptide using stop-anticodon and frameshift anticodon-containing tRNAs, which were acylated with PEG-containing amino acids.
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. y-ito@riken.jp.
Qingmin
Q
Zang
Seiichi
S
Tada
Takanori
T
Uzawa
Daisuke
D
Kiga
Masayuki
M
Yamamura
Yoshihiro
Y
Ito
D000215:Acylation; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics*; D000926:Anticodon; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000235:genetics*; D011092:Polyethylene Glycols / Q000737:chemistry*; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics*
D000596:Amino Acids; D000926:Anticodon; D010455:Peptides; D011092:Polyethylene Glycols; D012343:RNA, Transfer