The gastroprotective effect of pogostone from Pogostemonis Herba against indomethacin-induced gastric ulcer in rats.
Pogostemonis Herba, known as "Guang-Huo-Xiang" in Chinese, has been widely used in the treatment of gastrointestinal dysfunction. Pogostone is one of the major constituents of Pogostemonis Herba. The aim was to scientifically evaluate the possible gastroprotective effect and the underlying mechanisms of pogostone against indomethacin-induced gastric ulcer in rats. Rats were orally treated with vehicle, lansoprazole (30 mg/kg) or pogostone (10, 20 and 40 mg/kg) and subsequently exposed to acute gastric lesions induced by indomethacin. Gross evaluation, histological observation, gastric mucosal superoxide dismutase activity, glutathione content, catalase activity, malonaldehyde level and prostaglandin E2 production were performed. Immunohistochemistry and reverse transcription polymerase chain reaction for cyclooxygenase-1 and cyclooxygenase-2, as well as terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling assay, immunohistochemistry for heat-shock protein 70, B-cell lymphoma-2 and Bax were conducted. Results indicated that rats pretreated with pogostone showed remarkable protection from the gastric mucosa damage compared to vehicle-treated rats based on the ulcer index and inhibition percentage. Histologically, oral administration of pogostone resulted in observable improvement of gastric injury, characterized by reduction of necrotic lesion, flattening of gastric mucosa and alleviation of submucosal edema with hemorrhage. Pogostone pretreatment significantly raised the depressed activities of superoxide dismutase, glutathione and catalase, while reduced the elevated malonaldehyde level compared with indomethacin-induced group. Pogostone-pretreated group induced a significant increase in gastric mucosal prostaglandin E2 level and obvious up-regulation of protein levels and mRNA expressions of cyclooxygenase-1 and cyclooxygenase-2. Furthermore, antiapoptotic effect of pogostone was verified by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling assay, and the apoptotic process triggered by pogostone involved the up-expression of heat-shock protein70 and B-cell lymphoma-2 protein, and suppression of Bax protein expressions in the ulcerated tissues. It is speculated that the gastroprotective effect of pogostone against indomethacin-induced gastric ulceration might be associated with its stimulation of cyclooxygenase-mediated prostaglandin E2, antioxidant and antiapoptotic effect.
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Xiao-Ying
XY
Chen
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, People's Republic of China Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, People's Republic of China.
Hai-Ming
HM
Chen
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Yu-Hong
YH
Liu
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Zhen-Biao
ZB
Zhang
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Yi-Feng
YF
Zheng
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, People's Republic of China.
Zu-Qing
ZQ
Su
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Xie
X
Zhang
The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, People's Republic of China.
Jian-Hui
JH
Xie
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China Patent Examination Cooperation Center of the Patent Office, SIPO, Guangdong, Guangzhou, 510530, People's Republic of China.
Yong-Zhuo
YZ
Liang
Laboratory Animal Center of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523808, People's Republic of China.
Lu-Di
LD
Fu
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523808, People's Republic of China.
Xiao-Ping
XP
Lai
College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523808, People's Republic of China suziren@gzucm.edu.cn huangxiaoqi@gzucm.edu.cn.
Zi-Ren
ZR
Su
The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, People's Republic of China.
Xiao-Qi
XQ
Huang
D000284:Administration, Oral; D000818:Animals; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000473:pathology; D005765:Gastrointestinal Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D007213:Indomethacin / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008853:Microscopy; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000302:isolation & purification; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control*; D016896:Treatment Outcome
D005765:Gastrointestinal Agents; D009822:Oils, Volatile; C483658:Pogostone; D007213:Indomethacin
Pogostone; antiapoptosis; antioxidant; gastroprotective; indomethacin; prostaglandin E2; rats