Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation.
Isolated primary hepatocytes are considered as the reference system for in vitro hepatic methods. Following the isolation of primary hepatocytes from liver tissue, an unfavorable process named dedifferentiation is initiated leading to the attenuation of the hepatocellular phenotype both at the morphological and functional level. Freshly isolated hepatocytes can be used immediately or can be cryopreserved for future purposes. Currently, a number of antidedifferentiation strategies exist to extend the life span of isolated hepatocytes. The addition of differentiation-promoting compounds to the hepatocyte culture medium is the oldest and simplest antidedifferentiation approach applied. In the present chapter, the most commonly used medium additives for cultivation and cryopreservation of primary hepatocytes are reviewed.
EURL ECVAM, Systems Toxicology Unit, Institute for Health and Consumer Protection, European Commission, Joint Research Center, Via Fermi 2749, Ispra, 21027, Italy.
Varvara
V
Gouliarmou
Olavi
O
Pelkonen
Sandra
S
Coecke
D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques
D015415:Biomarkers; D003451:Cryoprotective Agents; D003470:Culture Media