An exogenous hydrogen sulphide donor, NaHS, inhibits the apoptosis signaling pathway to exert cardio-protective effects in a rat hemorrhagic shock model.
Hydrogen sulfide (H2S) has been reported to be interwined in multiple systems, specifically in the cardiovascular system. However, the mechanisms underlying remain controversial. In the present study, we assessed the cardio-protective effects of H2S in the rat hemorrhagic shock model. Hemorrhagic shock was induced in adult male Sprague-Dawley rats by drawing blood from the femoral artery to maintain the mean arterial pressure at 35-40 mmHg for 1.5 h. The rats were assigned to four groups and the H2S donor, NaHS (28 μmol/kg, i.p.), was injected before the resuscitation in certain groups. After resuscitation the animals were observed and then killed to harvest the hearts. The morphological investigation and ultrastructural analyses were done and apoptotic cells were detected. The levels of relevant proteins were examined using Western blotting and immunohistochemical analyses. Resuscitated hemorrhagic shock induced heart injury and significantly increased the levels of serum myocardial enzymes, creatine kinase (CK) and lactate dehydrogenase (LDH) levels. Furthermore, it caused marked increase of apoptotic cells in heart tissue. Moreover, the expression of death receptor Fas and Fas-ligand, as well as the expression of apoptosis-relevant proteins active-caspase 3 and active-caspase 8 were markedly increased. Administration of NaHS significantly ameliorated hemorrhagic shock caused hemodynamic deterioration, decreased myocardial enzymes elevation, protected myocardial ultrastructure, and inhibited the expression of apoptosis-relevant proteins. It suggested that H2S might exert its cardio-protective roles via both the extrinsic Fas/FasL/caspase-8/caspase-3 pathway and the intrinsic mitochondria-involved pathways.
Department of Oncology, The Third Affiliated Hospital of Soochow University Changzhou 213003, China.
Yanjie
Y
Xu
Department of Anesthesiology, The Third Affiliated Hospital of Soochow University Changzhou 213003, China.
Xiongwei
X
Dai
Department of Oncology, The Third Affiliated Hospital of Soochow University Changzhou 213003, China.
Danxia
D
Zhu
Department of Oncology, The Third Affiliated Hospital of Soochow University Changzhou 213003, China.
Xiaoli
X
Xu
Department of Anesthesiology, The Third Affiliated Hospital of Soochow University Changzhou 213003, China.
Cao
C
Gao
Department of Oncology, The Third Affiliated Hospital of Soochow University Changzhou 213003, China.
Changping
C
Wu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects*; D006439:Hemodynamics / Q000187:drug effects; D006862:Hydrogen Sulfide / Q000378:metabolism; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology*
D002316:Cardiotonic Agents; D013440:Sulfides; C025451:sodium bisulfide; D006862:Hydrogen Sulfide
Hydrogen sulphide; apoptosis; cardio-protective effect; hemorrhagic shock