Cell therapy in the treatment of bronchiolitis obliterans in a murine model.
OBJECTIVE
To evaluate the importance of stem cells derived from adipose tissue in reducing graft inflammation in a murine model of allogeneic heterotopic tracheal transplant.


METHODS
We performed a heterotopic tracheal allografting in dorsal subcutaneous pouch and systemically injected 5x105 mesenchymal stem cells derived from adipose tissue. The animals were divided into two groups according to the time of sacrifice: T7 and T21. We also carried out histological analysis and digital morphometry.


RESULTS
The T7 animals treated with cell therapy had median obstructed graft area of 0 versus 0.54 of controls (p = 0.635). The treated T21 subjects had median obstructed graft area of 0.25 versus 0 in controls (p = 0.041).


CONCLUSIONS
The systemically injected cell therapy in experimental murine model of bronchiolitis obliterans did not reduce the severity of the allograft inflammation in a statistically significant way in seven days; Conversely, in 21 days, it increased the allograft inflammatory process.
Universidade Federal do Rio Grande do Sul, RS, BR.
Julio de Oliveira
Jde O
Espinel
Universidade Federal do Rio Grande do Sul, RS, BR.
Carolina
C
Uribe
Universidade Federal do Rio Grande do Sul, RS, BR.
Fabíola Schons
FS
Meyer
Universidade Federal de Ciências da Saúde de Porto Alegre, RS, BR.
Rafael
R
Bringheti
Universidade Federal do Rio Grande do Sul, RS, BR.
Jane Ulbricht
JU
Kulczynski
Universidade Federal do Rio Grande do Sul, RS, BR.
Maurício Guidi
MG
Saueressig
D000818:Animals; D001989:Bronchiolitis Obliterans / Q000601:surgery*; D004195:Disease Models, Animal; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice