NMR study on the interaction of the conserved CREX 'stem-loop' in the Hepatitis E virus genome with a naphthyridine-based ligand.
A 2-amino-1,8-naphthyridine derivative that is described to bind single guanine bulges in RNA-DNA and RNA-RNA duplexes was synthesized and its interaction with the single G bulge in the conserved CREX of the Hepatitis E Virus (HEV) genome was explored by NMR and molecular modeling. Results indicate that the ligand intercalates in the internal loop, though none of the expected hydrogen bonds with the single G in the bulge could be demonstrated.
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium. Eveline.Lescrinier@rega.kuleuven.be.
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N
Dyubankova
M
M
Froeyen
M
M
Abramov
H P
HP
Mattelaer
P
P
Herdewijn
E
E
Lescrinier
D020029:Base Pairing; D001483:Base Sequence; D017124:Conserved Sequence; D016679:Genome, Viral / Q000235:genetics*; D016752:Hepatitis E virus / Q000235:genetics*; D055029:Inverted Repeat Sequences; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009287:Naphthyridines / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
C427025:2-amino-1,8-naphthyridine; D008024:Ligands; D009287:Naphthyridines; D012367:RNA, Viral