Expression of feruloyl esterase A from Aspergillus terreus and its application in biomass degradation.
Feruloyl esterases (FAEs) are key enzymes involved in the complete biodegradation of lignocelluloses, which could hydrolyze the ester bonds between hemicellulose and lignin. The coding sequence of a feruloyl esterase A (AtFaeA) was cloned from Aspergillus terreus and the recombinant AtFaeA was constitutively expressed in Pichia pastoris. The SDS-PAGE analysis of purified AtFaeA showed two protein bands owing to the different extent of glycosylation, and the recombinant AtFaeA had an optimum temperature of 50°C and an optimum pH of 5.0. The substrate utilization and primary sequence identity of AtFaeA demonstrated that it is a type-A feruloyl esterase. The hydrolysis of corn stalk and corncob by xylanase from Aspergillus niger could be significantly improved in concert with recombinant AfFaeA.
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China. Electronic address: shbzhang@163.com.
Shuai-Bing
SB
Zhang
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Le
L
Wang
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, China.
Yan
Y
Liu
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Huan-Chen
HC
Zhai
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Jing-Ping
JP
Cai
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Yuan-Sen
YS
Hu
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D001673:Biodegradation, Environmental; D018533:Biomass; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D008969:Molecular Sequence Data; D010843:Pichia; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D016415:Sequence Alignment; D003313:Zea mays
D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase
Aspergillus terreus; Feruloyl esterase; Lignocelluloses biodegradation; Pichia pastoris