Identification of Recurrence-Related microRNAs from Bone Marrow in Hepatocellular Carcinoma Patients.
Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14(+) (macrophage), CD14(-)/CD45(+) (lymphocyte), and CD14(-)/CD45(-)/EpCAM(+) (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. sugimachi@beppu.kyushu-u.ac.jp.|Department of Surgery, Fukuoka City Hospital, 13-1 Yoshizukahonmachi, Fukuoka 812-0046, Japan. sugimachi@beppu.kyushu-u.ac.jp.
Keishi
K
Sugimachi
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. z33sho2000@yahoo.co.jp.
Shotaro
S
Sakimura
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. akiratomokuni@gmail.com.
Akira
A
Tomokuni
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. ryuuchi@beppu.kyushu-u.ac.jp.
Ryutaro
R
Uchi
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. shusei_1300g7m7s@yahoo.co.jp.
Hidenari
H
Hirata
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. komateru8312@gmail.com.
Hisateru
H
Komatsu
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. yshinden@beppu.kyushu-u.ac.jp.
Yoshiaki
Y
Shinden
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. tomo@surg2.med.kyushu-u.ac.jp.
Tomohiro
T
Iguchi
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. heguchi@beppu.kyushu-u.ac.jp.
Hidetoshi
H
Eguchi
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. takaakimas@yahoo.co.jp.
Takaaki
T
Masuda
Department of Surgery, Fukuoka City Hospital, 13-1 Yoshizukahonmachi, Fukuoka 812-0046, Japan. kamorita@surg2.med.kyushu-u.ac.jp.
Kazutoyo
K
Morita
Department of Surgery and Science Graduate School of Medical Sciences Kyushu University 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. kshirabe@surg2.med.kyushu-u.ac.jp.
Ken
K
Shirabe
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. heguchi@gesurg.med.osaka-u.ac.jp.
Hidetoshi
H
Eguchi
Department of Surgery and Science Graduate School of Medical Sciences Kyushu University 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. maehara@surg2.med.kyushu-u.ac.jp.
Yoshihiko
Y
Maehara
Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. kmimori@beppu.kyushu-u.ac.jp.
Masaki
M
Mori
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. mmori@gesurg.med.osaka-u.ac.jp.
Koshi
K
Mimori
bone marrow; hepatocellular carcinoma; microRNA; recurrence