Aneusomy detection with Karyolite-Bac on Beads® is a cost-efficient and high throughput strategy in the molecular analyses of the early pregnancy conception losses.
BACKGROUND
Approximately 10-15 % of all clinically recognized pregnancies end in miscarriage, the majority of them occur during the first trimester, underlying the cause of the loss. Genetic analysis of fetal tissues has the potential to provide valuable information and is highly recommended in some cases. Around 3-4 years ago, the gold standard for the analysis was the GTG Kayrotype, is well known that around 50 % of the tissue samples received failed to grow in culture. Different molecular techniques are used to improve the quality and the specificity of the study, intending to circumvent the limits of the Karyotype.


RESULTS
Karyolite-BoBs™ (KL-BoB™) assay is a recent bead-based suspension, low density array technology with consistent results, probed that is an efficient molecular method to detect aneusomies in early pregnancy losses. Fifty samples from abortions were analyzed in order to probe and give more information about the methodology and analyze if KL-BoBs™ is a good and cost-efficient strategy. We detected 32 % of chromosomal abnormalities, in some of the cases more than one aberration was identified, the array CGH validate the observations.


CONCLUSIONS
This molecular strategy is a cost-effective sensitive tool in the early pregnancy loss study.
Doctorado en Ciencias Quimicobiológicas, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, Mexico ; Nanolab Genetic Center, Molecular Biology Laboratory, México City, Mexico.
Javier
J
Pérez-Durán
Nanolab Genetic Center, Molecular Biology Laboratory, México City, Mexico.
Zenyese
Z
Nájera
Nanolab Genetic Center, Molecular Biology Laboratory, México City, Mexico.
Yanelly
Y
Trujillo-Cabrera
Human Genetic Department, Hospital Materno Infantil ISSEMyM, Estado de México, Mexico.
Mónica
M
Martín-Saro
Laboratorio de Inmunoquímica I, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, Mexico.
Ethel
E
García-Latorre
IECH, Fertility Center, Chihuahua, Chihuahua Mexico.
Jaime
J
Escarcega-Preciado
Nanolab Genetic Center, Molecular Biology Laboratory, México City, Mexico.
Nayelli
N
Nájera
Nanolab Genetic Center, Molecular Biology Laboratory, México City, Mexico.
Teresa
T
Martínez-Galaviz
Nanolab Genetic Center, Molecular Biology Laboratory, México City, Mexico ; Human Genetics Department, Hospital General de Mexico, Facultad de Medicina Universidad Nacional Autonoma de Mexico, Dr. Balmis 142 Col, Doctores CP, 06766 Mexico City, Mexico DF Mexico.
Gloria
G
Queipo