Genetic polymorphisms of the AMPD1 gene and their correlations with IMP contents in Fast Partridge and Lingshan chickens.
The object of this study was to evaluate associations between the adenosine monophosphate deaminase 1 (AMPD1) gene polymorphisms and inosine monophosphate acid (IMP) contents of chicken to provide a molecular marker for breeding. Three single nucleotide polymorphisms (SNPs), g.4064G/A, g.5573A/G and g.6805G/A were detected in exons IV, VI, and VIII of the AMPD1 gene in Fast Partridge and Lingshan chickens, respectively. All were purine conversion and caused no alteration in amino acid sequence. Statistical analysis revealed that Lingshan chicken with the homozygous genotype AA at position 4064 and 6805 had a significantly greater IMP content than those with the GG genotype (P<0.05). Fast Partridge chicken with the genotype GG at position 6805 had a significantly greater IMP content compared with those with the AA genotype (P<0.05). In conclusion, the polymorphism at g.6805A/G was correlated with IMP content (P<0.05) in both Fast Partridge and Lingshan chickens. The results in our study suggest that SNP 6805A/G can be used as a possible candidate marker of IMP content of chicken.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: 2013205009@njau.edu.cn.
Jin
J
Hu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: 550619781@qq.com.
Ping
P
Yu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: 2011205009@njau.edu.cn.
Xiaoling
X
Ding
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: 2012105040@njau.edu.cn.
Minglong
M
Xu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: 2012105038@njau.edu.cn.
Baoping
B
Guo
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: xuyinxue@njau.edu.cn.
Yinxue
Y
Xu
D000659:AMP Deaminase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D056726:Genetic Association Studies; D005819:Genetic Markers; D007291:Inosine Monophosphate / Q000378:metabolism*; D008460:Meat; D016366:Open Reading Frames / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014018:Tissue Distribution
D005819:Genetic Markers; D007291:Inosine Monophosphate; D000659:AMP Deaminase
AMPD1; Fast Partridge chicken; IMP; Lingshan chicken; SNP