Identification of Rab6a as a New Target of microRNA-155 Involved in Regulating Lipopolysaccharide-Induced TNF Secretion.
This study aims to provide experimental proof that Rab6a is an efficient target of microRNA-155 in regulating pro-inflammatory tumor necrosis factor (TNF) secretion stimulated by lipopolysaccharide (LPS) in macrophages. We identified Rab6a as a new target of microRNA-155 (miR-155) and found that overexpression of miR-155 decreased Rab6a expression at both protein and mRNA levels, which resulted in a significant reduction of TNF secretion induced by lipopolysaccharide stimulation. We have demonstrated that miR-155 can negatively regulate inflammatory TNF secretion in lipopolysaccharide stimulated macrophages, partly by targeting Rab6a, thereby providing new insights into the role of miR-155 in cytokine secretion in inflammatory macrophages.
Department of Postgraduate, Third Military Medical University, Chongqing, 400038, China.
Yang
Y
Yang
Department of Postgraduate, Third Military Medical University, Chongqing, 400038, China. doctorlxyang@163.com.|Department of Cardiology, Kunming General Hospital of Chengdu Military Area, Yunnan, 650032, China. doctorlxyang@163.com.
Lixia
L
Yang
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000235:genetics*; D007249:Inflammation / Q000235:genetics; D008070:Lipopolysaccharides; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*
D008070:Lipopolysaccharides; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C094135:Rab6 protein; D014409:Tumor Necrosis Factor-alpha; D020691:rab GTP-Binding Proteins
LPS; Rab6a; inflammatory TNF; microRNA-155