SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
Endothelial exocytosis regulates vascular thrombosis and inflammation. The trafficking and release of endothelial vesicles is mediated by SNARE (Soluble NSF Attachment protein REceptors) molecules, but the exact identity of endothelial SNAREs has been unclear. Three SNARE molecules form a ternary complex, including isoforms of the syntaxin (STX), vesicle-associated membrane protein (VAMP), and synaptosomal-associated protein (SNAP) families. We now identify SNAP23 as the predominant endothelial SNAP isoform that mediates endothelial exocytosis of von Willebrand Factor (VWF). SNAP23 was localized to the plasma membrane. Knockdown of SNAP23 decreased endothelial exocytosis, suggesting it is important for endothelial exocytosis. SNAP23 interacted with the endothelial exocytic machinery, and formed complexes with other known endothelial SNARE molecules. Taken together, these data suggest that SNAP23 is a key component of the endothelial SNARE machinery that mediates endothelial exocytosis.
Qiuyu  Martin
Q
Zhu
Aab Cardiovascular Research Institute, Department of Medicine, University of Rochester Medical Center, Rochester, New York, United States of America; Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
Qiuyu
Q
Zhu
Aab Cardiovascular Research Institute, Department of Medicine, University of Rochester Medical Center, Rochester, New York, United States of America.
Munekazu
M
Yamakuchi
Aab Cardiovascular Research Institute, Department of Medicine, University of Rochester Medical Center, Rochester, New York, United States of America.
Charles J
CJ
Lowenstein
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D005089:Exocytosis; D015870:Gene Expression; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D011485:Protein Binding; D021381:Protein Transport; D050766:Qb-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D050767:Qc-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism*
D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C100493:SNAP23 protein, human; D014841:von Willebrand Factor