Use of the 2-chlorotrityl chloride resin for microwave-assisted solid phase peptide synthesis.
A fast and efficient microwave (MW)-assisted solid-phase peptide synthesis protocol using the 2-chlorotrityl chloride resin and the Fmoc/tBu methodology, has been developed. The established protocol combines the advantages of MW irradiation and the acid labile 2-chlorotrityl chloride resin. The effect of temperature during the MW irradiation, the degree of resin substitution during the coupling of the first amino acids and the rate of racemization for each amino acid were evaluated. The suggested solid phase methodology is applicable for orthogonal peptide synthesis and for the synthesis of cyclic peptides.
Department of Chemistry, University of Patras, 26504, Rion, Greece.
Matthaia
M
Ieronymaki
Department of Chemistry, University of Patras, 26504, Rion, Greece.|Eldrug S.A., Pharmaceutical Company, 26504, Platani, Greece.
Maria Eleni
ME
Androutsou
Department of Chemistry, University of Patras, 26504, Rion, Greece.
Anna
A
Pantelia
Department of Chemistry, University of Patras, 26504, Rion, Greece.|Eldrug S.A., Pharmaceutical Company, 26504, Platani, Greece.
Irene
I
Friligou
BioArCh, Department of Chemistry, University of York, YO10 5DD, United Kingdom.
Molly
M
Crisp
BioArCh, Department of Chemistry, University of York, YO10 5DD, United Kingdom.
Kirsty
K
High
BioArCh, Department of Chemistry, University of York, YO10 5DD, United Kingdom.
Kirsty
K
Penkman
Department of Chemistry, University of Patras, 26504, Rion, Greece.
Dimitrios
D
Gatos
Department of Chemistry, University of Patras, 26504, Rion, Greece.
Theodore
T
Tselios
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D008872:Microwaves; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques; D013696:Temperature; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
D010456:Peptides, Cyclic; D014320:Triphenylmethyl Compounds; C071065:2-chlorotrityl chloride
solid phase peptide synthesis, microwave assisted peptide synthesis, 2-chlorotrityl chloride resin, racemization