Dietary Selenium Status Regulates the Transcriptions of Selenoproteome and Activities of Selenoenzymes in Chicken Kidney at Low or Super-nutritional Levels.
To determine dietary selenium (Se) status regulates the transcriptions of selenoproteome and activities of selenoenzymes in chicken kidney, 1-day-old chickens received low Se (0.028 mg Se per kg of diet) or super-nutritional Se (3.0 or 5.0 mg Se per kg of diet) in their diets for 8 weeks. It was observed that dietary low or super-nutritional Se did not make renal appearance pathological changes in chicken. Low Se significantly reduced total antioxidant capability (T-AOC), glutathione (GSH) content, but malondialdehyde (MDA) content in the kidney increased and decreased glutathione peroxidase (Gpx) and thioredoxin reductase (TrxR) activity with changes in their mRNA levels. Super-nutritional Se (3.0 mg/kg) increased T-AOC and GSH contents then made them reduce, but it reduced MDA content significantly, elevated then reduced Gpx activity, and decreased TrxR activity with changes in their mRNA levels. Dietary low Se downregulated the mRNA expressions of Gpx1-4, Txnrd3, Sepn1, Selw, Sepx1, Selh, and SEPSECS. At super-nutritional Se, most selenoproteins were upregulated in chicken kidney, but Sepp2 and Sep15 was only upregulated in Se excess (5.0 mg/kg) bird. These results indicated that dietary Se status stabilizes normal renal physiology function via regulation of the selenoprotemic transcriptions and selenoenzyme activities in avian.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Jing-Xiu
JX
Xu
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Cong
C
Zhang
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Chang-Yu
CY
Cao
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Shi-Yong
SY
Zhu
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Hui
H
Li
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, People's Republic of China.
Yan-Chun
YC
Sun
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. jinlongli@neau.edu.cn.
Jin-Long
JL
Li
D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis*; D002645:Chickens; D019587:Dietary Supplements; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007668:Kidney / Q000378:metabolism*; D012643:Selenium / Q000494:pharmacology*; D051140:Selenoproteins / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects*
D030161:Avian Proteins; D051140:Selenoproteins; D012643:Selenium
Chicken; Kidney; Low or super-nutritional selenium; Selenoenzymes activities; Selenoproteome transcriptions