A novel direct spray-from-tissue ionization method for mass spectrometric analysis of human brain tumors.
Real-time feedback about dissected tissue during the neurosurgical procedure is strongly requested. A novel direct ionization mass spectrometric method for identifying pathological differences in tissues is proposed. The method is based on simultaneous extraction of tissue lipids and electrospray ionization which allows mass spectrometric data to be obtained directly from soft tissues. The advantage of this method is the stable flow of solvent, which leads to stable time-dependent spectra. The tissues included necrotized tissues and tumor tissues in different combinations. Capability for direct analysis of samples of dissected tissues during the neurosurgical procedure is demonstrated. Data validation is conducted by compound identification using precise masses from the MS profile, MS/MS, and isotopic distribution structure analysis. The method can be upgraded and applied for real-time identification of tissues during surgery. This paper describes the technique and its application perspective. For these purposes, other methods were compared with the investigated one and the results were shown to be reproducible. Differences in lipid profiles were observed even in tissues from one patient where distinctions between different samples could be poor. The paper presents a proof of concept for the method to be applied in neurosurgery particularly and in tissue analysis generically. The paper also contains preliminary results proving the possibility of observing differences in mass spectra of different tumors.
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, Institutskij bystr. 9, 141700, Dolgoprudnyi, Moscow Region, Russia.|Research Center for Obstetrics, Gynecology and Perinatology, Oparina st. 4, 117997, Moscow, Russia.
Alexey
A
Kononikhin
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, Institutskij bystr. 9, 141700, Dolgoprudnyi, Moscow Region, Russia.
Evgeny
E
Zhvansky
Burdenko Neurosurgical Institute of the Russian Academy of Medical Sciences, 4-th Tverskaya-Yamskaya st. 16, 125047, Moscow, Russia.
Vsevolod
V
Shurkhay
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Emanuel Institute for Biochemical Physics of the Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, Institutskij bystr. 9, 141700, Dolgoprudnyi, Moscow Region, Russia.
Igor
I
Popov
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, Institutskij bystr. 9, 141700, Dolgoprudnyi, Moscow Region, Russia.
Denis
D
Bormotov
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, Institutskij bystr. 9, 141700, Dolgoprudnyi, Moscow Region, Russia.|Skolkovo Institute of Science and Technology, Novaya St., 100, 143025, Skolkovo, Russian Federation.
Yury
Y
Kostyukevich
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.
Sofiia
S
Karchugina
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Emanuel Institute for Biochemical Physics of the Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia.
Maria
M
Indeykina
Emanuel Institute for Biochemical Physics of the Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia.|Research Center for Obstetrics, Gynecology and Perinatology, Oparina st. 4, 117997, Moscow, Russia.
Anna
A
Bugrova
Moscow Institute of Physics and Technology, Institutskij bystr. 9, 141700, Dolgoprudnyi, Moscow Region, Russia.|Research Center for Obstetrics, Gynecology and Perinatology, Oparina st. 4, 117997, Moscow, Russia.
Natalia
N
Starodubtseva
Burdenko Neurosurgical Institute of the Russian Academy of Medical Sciences, 4-th Tverskaya-Yamskaya st. 16, 125047, Moscow, Russia.
Alexander
A
Potapov
Institute for Energy Problems of Chemical Physics of the Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia. ennikolaev@rambler.ru.|Emanuel Institute for Biochemical Physics of the Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia. ennikolaev@rambler.ru.|Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Pogodinskaya st. 10, 119121, Moscow, Russia. ennikolaev@rambler.ru.|Skolkovo Institute of Science and Technology, Novaya St., 100, 143025, Skolkovo, Russian Federation. ennikolaev@rambler.ru.
Eugene
E
Nikolaev
D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry* / Q000473:pathology*; D004867:Equipment Design; D006801:Humans; D008055:Lipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D008055:Lipids
Ambient mass spectrometry; Brain; Diagnostics; Electrospray; FT ICR; Tumor