Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71.
An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L(-1). Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg(-1)) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D.
The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China. ; Guizhou University, College of Life Sciences, Guizhou University, Guiyang, China, College of Life Sciences, Guizhou University, Guiyang, China. ; Guizhou University, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China.
Lizhen
L
Han
The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China. ; Guizhou University, College of Life Sciences, Guizhou University, Guiyang, China, College of Life Sciences, Guizhou University, Guiyang, China. ; Guizhou University, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China.
Degang
D
Zhao
Guizhou University, College of Life Sciences, Guizhou University, Guiyang, China, College of Life Sciences, Guizhou University, Guiyang, China. ; Guizhou University, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China.
Cuicui
C
Li
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D001431:Bacteriological Techniques; D001711:Biotransformation; D002681:China; D016000:Cluster Analysis; D049921:Cupriavidus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006540:Herbicides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism; D011810:Quinoxalines / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D013997:Time Factors; D014908:Triticum
D000085:Acetates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006540:Herbicides; D011422:Propionates; D011725:Pyridines; D011810:Quinoxalines; D012336:RNA, Ribosomal, 16S; C477202:quizalofop; D015084:2,4-Dichlorophenoxyacetic Acid; C416382:4,5-amino-3,5-dichloro-6-fluoro-2-pyridinyloxyacetic acid
2,4-D-degrading bacterial strain; Cupriavidus campinensis BJ71; bioremediation; degradation characteristics