Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants.
OBJECTIVE
To obtain micro propagated Uncaria tomentosa plantlets with enhanced secondary metabolites production, long-term responses to salicylic acid (SA) pre-treatments at 1 and 100 µM were evaluated after propagation of the plantlets in a SA-free medium.


RESULTS
SA pre-treatments of single node cuttings OF U. tomentosa produced long-term responses in microplants grown for 75 days in a SA-free medium. Reduction in survival rate, root formation, and stem elongation were observed only with 100 µM SA pre-treatments with respect to the control (0 + DMSO).Both pre-treatments enhanced H2O2 and inhibited superoxide dismutase and catalase activities, while guaiacol peroxidase was increased only with 1 µM SA. Also, both pre-treatments increased total monoterpenoid oxindole alkaloids by ca. 55 % (16.5 mg g(-1) DW), including isopteropodine, speciophylline, mitraphylline, isomitraphylline, rhynchopylline, and isorhynchopylline; and flavonoids by ca. 21 % (914 μg g(-1) DW), whereas phenolic compounds were increased 80 % (599 μg g(-1) DW) at 1 µM and 8.2 % (359 μg g(-1) DW) at 100 µM SA.


CONCLUSIONS
Pre-treatment with 1 µM SA of U.tomentosa microplants preserved the survival rate and increased oxindole alkaloids, flavonoids, and phenolic compounds in correlation with H2O2 and peroxidase activity enhancements, offering biotechnological advantages over non-treated microplants.
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico.
Silvia
S
Sánchez-Rojo
Departamento de Química, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico.
Carlos M
CM
Cerda-García-Rojas
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico.
Fernando
F
Esparza-García
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, D.F., Mexico.
Javier
J
Plasencia
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico.
Héctor M
HM
Poggi-Varaldo
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico.
Teresa
T
Ponce-Noyola
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico. aramos@cinvestav.mx.
Ana C
AC
Ramos-Valdivia
D000470:Alkaloids / Q000032:analysis; D000975:Antioxidants / Q000378:metabolism*; D020948:Cat's Claw / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D007211:Indoles / Q000032:analysis; D039821:Monoterpenes / Q000032:analysis; D000078183:Oxindoles; D010636:Phenols / Q000032:analysis; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D020156:Salicylic Acid / Q000378:metabolism*; D064210:Secondary Metabolism / Q000187:drug effects*; D016019:Survival Analysis
D000470:Alkaloids; D000975:Antioxidants; D003470:Culture Media; D005419:Flavonoids; D007211:Indoles; D039821:Monoterpenes; D000078183:Oxindoles; D010636:Phenols; C022960:2-oxindole; D006861:Hydrogen Peroxide; D020156:Salicylic Acid
Antioxidant enzymes; Long-term response; Monoterpenoid oxindole alkaloids; Phenolic compounds; Salicylic acid; Uncaria tomentosa