Quencher group induced high specificity detection of telomerase in clear and bloody urines by AIEgens.
Telomerase is a widely used tumor biomarker for early cancer diagnosis. On the basis of the combined use of aggregation-induced emission (AIE) fluorogens and quencher, a quencher group induced high specificity strategy for detection of telomerase activity from cell extracts and cancer patients' urine specimens was creatively developed. In the absence of telomerase, fluorescence background is extremely low due to the short distance between quencher and AIE dye. In the addition of telomerase, fluorescence enhances significantly. The telomerase activity in the E-J, MCF-7, and HeLa extracts equivalent to 5-10 000 cells can be detected by this method in ∼1 h. Furthermore, the distinguishing of telomerase extracted from 38 cancer and 15 normal urine specimens confirms the reliability and practicality of this protocol. In contrast to our previous results (Anal. Chem. 2015, 87, 6822-6827), these advanced experiments obtain more remarkable specificity.
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Yuan
Y
Zhuang
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Mengshi
M
Zhang
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
Bin
B
Chen
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Ruixue
R
Duan
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Xuehong
X
Min
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Zhenyu
Z
Zhang
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, China.
Fuxin
F
Zheng
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, China.
Huageng
H
Liang
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
Zujin
Z
Zhao
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Xiaoding
X
Lou
Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
Fan
F
Xia
D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D006417:Hematuria / Q000150:complications / Q000201:enzymology / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence; D019098:Telomerase / Q000652:urine*; D016482:Urinalysis / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000150:complications / Q000175:diagnosis / Q000201:enzymology / Q000652:urine; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives / Q000737:chemistry
D005456:Fluorescent Dyes; C063127:4-(4-dimethylaminophenylazo)benzoic acid; D004124:p-Dimethylaminoazobenzene; D019098:Telomerase