Diethylstilbestrol affects the expression of GPER in the gubernaculum testis.
Recent evidence suggested a positive correlation between environmental estrogens (EEs) and high incidence of abnormalities in male urogenital system. EEs are known to cause the abnormalities of testes development and testicular descent. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernacular cells, and its nongenomic effects on gubernaculum testis cells may be mediated by G protein-coupled estrogen receptor (GPER). In this study, we detected the expression of GPER in mouse gubernacular testis and investigated the effects of DES on the expression of GPER in gubernaculum testis cells. RT-PCR analysis revealed that GPER mRNA was expressed in the gubernaculum. GPER protein was detected in the parenchymal cells of the gubernaculum early in development. Furthermore, we demonstrate that GPER inhibitor G15 relieved DES-induced inhibition of GPER expression in gubernaculum testis cell, but ER inhibitor ICI 182780 had the converse effects on DES-induced inhibition of GPER expression in these cells. These data suggest that the effects of DES on mouse gubernaculum testis cells are mediated at least partially by the regulation of GPER expression.
Department of Pediatric Surgery, The Affiliated Maternal and Child Health Hospital of Shenzhen University Medical College Shenzhen, Guangdong, China.
Xuan
X
Zhang
Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
Song
S
Ke
Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
Kai-Hong
KH
Chen
Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
Jian-Hong
JH
Li
Department of Pediatric, The Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
Lian
L
Ma
Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
Xue-Wu
XW
Jiang
D000818:Animals; D052117:Benzodioxoles / Q000494:pharmacology; D002478:Cells, Cultured; D004054:Diethylstilbestrol / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D065171:Estrogen Receptor Antagonists / Q000494:pharmacology; D000077267:Fulvestrant; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D011804:Quinolines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
C568862:4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta(c)quinoline; D052117:Benzodioxoles; D052244:Endocrine Disruptors; D065171:Estrogen Receptor Antagonists; C532507:GPER1 protein, mouse; D011804:Quinolines; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D000077267:Fulvestrant; D004958:Estradiol; D004054:Diethylstilbestrol
DES; EEs; GPER; gubernaculums testis