Pregnancy Rates to Fixed Embryo Transfer of Vitrified IVP Bos indicus, Bos taurus or Bos indicus × Bos taurus Embryos.
The pregnancy rates obtained after the transfer of cryopreserved in vitro-produced (IVP) embryos are usually low and/or inconsistent. The objective of this study was to evaluate the pregnancy rates of Holstein, Gyr and Holstein × Gyr cattle after the transfer of vitrified IVP embryos produced with X-sorted sperm. Seventy-two Gyr and 703 Holstein females were subjected to ovum pickup (OPU) sessions, followed by in vitro embryo production using semen from sires of the same breeds. Embryos (1636 Holstein, 241 Gyr and 1515 Holstein × Gyr) were exposed to forskolin for 48 h prior to vitrification. The pregnancy rate achieved with Gyr dam and sire was 46.1%, which was similar (p = 0.11) to that of Holstein dam and Gyr sire (40.3%). Crossing Gyr dams with Holstein sires resulted in a pregnancy rate of 38.9% and did not differ (p = 0.58) from the pregnancy rate obtained with the cross between Holstein dams and Gyr sires. The rate obtained with Holstein dam and sire was 32.5%. The average pregnancy rate was 36.6%, and no difference was found in the proportion of female foetuses (88.8%, in average) among breeds (p > 0.05). In conclusion, transfer of cryopreserved X-sorted embryos represents an interesting choice for dairy cattle. Despite the small differences between pregnancy rates, we highlight the efficiency of this strategy for all of the racial groups studied.
Laboratório de Reprodução Animal, State University of Londrina (UEL), Londrina, PR, Brazil.
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LS
Marinho
Laboratório de Reprodução Animal, State University of Londrina (UEL), Londrina, PR, Brazil.|In Vitro Brasil Ltda, Mogi Mirim, SP, Brazil.
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BV
Sanches
Laboratório de Reprodução Animal, State University of Londrina (UEL), Londrina, PR, Brazil.
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Rosa
In Vitro Brasil Ltda, Mogi Mirim, SP, Brazil.
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JH
Tannura
In Vitro Brasil Ltda, Mogi Mirim, SP, Brazil.
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AG
Rigo
In Vitro Brasil Ltda, Mogi Mirim, SP, Brazil.
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Basso
In Vitro Brasil Ltda, Mogi Mirim, SP, Brazil.
J H F
JH
Pontes
Laboratório de Reprodução Animal, State University of Londrina (UEL), Londrina, PR, Brazil.
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Seneda
D000818:Animals; D001947:Breeding; D002417:Cattle / Q000196:embryology* / Q000235:genetics / Q000502:physiology; D015925:Cryopreservation / Q000662:veterinary*; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D006358:Hot Temperature; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012743:Sex Preselection / Q000662:veterinary; D013045:Species Specificity; D013094:Spermatozoa