Identification of the mitochondrially encoded subunit 6 of F1FO ATPase in Trypanosoma brucei.
Kinetoplast maxicircle DNA of trypanosomatids encodes eighteen proteins. RNA editing is required to confer translatability to mRNA for twelve of these. Sequence conservation of the predicted hydrophobic polypeptides indicates that they represent functional components of the respiratory chain. Yet, so far only two of those, cytochrome c oxidase subunit I and apocytochrome b of cytochrome c reductase, have been identified with biochemical methods. Here we report on identification of A6 subunit of F1FO ATPase encoded by a pan-edited mRNA in Trypanosoma brucei. The polypeptide was present among the (35)S-labeled mitochondrial translation products characterized by anomalous migration in denaturing 2D gels. It was identified as an ATPase subunit by co-migration with this complex in Blue Native 2D gels. A partial N-terminal sequence of the corresponding polypeptide present in the gel-purified ATPase complex from Leishmania tarentolae was consistent with the predicted A6 sequence.
Department of Biology, University of California - Riverside, Riverside, CA 92521, USA; Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia.
Ingrid
I
Škodová-Sveráková
Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia.
Anton
A
Horváth
Department of Biology, University of California - Riverside, Riverside, CA 92521, USA. Electronic address: maslov@ucr.edu.
Dmitri A
DA
Maslov
D058645:Denaturing Gradient Gel Electrophoresis; D015180:Electrophoresis, Gel, Two-Dimensional; D007553:Isotope Labeling; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics*; D021122:Protein Subunits / Q000032:analysis / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000235:genetics*; D020539:Sequence Analysis, Protein; D014346:Trypanosoma brucei brucei / Q000201:enzymology*
D024101:Mitochondrial Proteins; D021122:Protein Subunits; D006180:Proton-Translocating ATPases
F(1)F(O) ATPase; Mitochondrial translation; RNA editing; Subunit 6; Trypanosoma brucei