Toxin Profile, Biofilm Formation, and Molecular Characterization of Emetic Toxin-Producing Bacillus cereus Group Isolates from Human Stools.
Emetic toxin-producing Bacillus cereus group species are an important problem, because the staple food for Korean is grains such as rice. In this study, we determined the prevalence (24 of 129 isolates) of emetic B. cereus in 36,745 stool samples from sporadic food-poisoning cases in Korea between 2007 and 2008. The toxin gene profile, toxin production, and biofilm-forming ability of the emetic B. cereus isolates were investigated. Repetitive element sequence polymorphism polymerase chain reaction fingerprints (rep-PCR) were also used to assess the intraspecific biodiversity of these isolates. Emetic B. cereus was present in 0.07% of the sporadic food-poisoning cases. The 24 emetic isolates identified all carried the nheABC and entFM genes and produced NHE enterotoxin. However, they did not have hemolysin BL toxin or related genes. A relationship between biofilm formation and toxin production was not observed in this study. The rep-PCR fingerprints of the B. cereus isolates were not influenced by the presence of toxin genes, or biofilm-forming ability. The rep-PCR assay discriminated emetic B. cereus isolates from nonemetic isolates, even if this assay did not perfectly discriminate these isolates. Further study on emetic isolates possessing a high degree of diversity may be necessary to evaluate the performance of the subtyping assay to discriminate emetic and nonemetic B. cereus isolates and could provide a more accurate indication of the risk from B. cereus strains.
1 Research Group of Gut Microbiome, Korea Food Research Institute , Gyeonggi, Korea.
Su Kyung
SK
Oh
2 Research Group of Food Safety, Korea Food Research Institute , Gyeonggi, Korea.
Hyun-Joo
HJ
Chang
2 Research Group of Food Safety, Korea Food Research Institute , Gyeonggi, Korea.
Sung-Wook
SW
Choi
2 Research Group of Food Safety, Korea Food Research Institute , Gyeonggi, Korea.
Gyeongsik
G
Ok
1 Research Group of Gut Microbiome, Korea Food Research Institute , Gyeonggi, Korea.
Nari
N
Lee
D001409:Bacillus cereus / Q000302:isolation & purification / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D004639:Emetics / Q000032:analysis; D004768:Enterotoxins / Q000032:analysis / Q000235:genetics*; D005243:Feces / Q000382:microbiology*; D005517:Foodborne Diseases / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D056910:Republic of Korea
D004639:Emetics; D004768:Enterotoxins; D006460:Hemolysin Proteins; C040792:enterotoxin, Bacillus cereus