Omega-3-fatty acid adds to the protective effect of flax lignan concentrate in pressure overload-induced myocardial hypertrophy in rats via modulation of oxidative stress and apoptosis.
Objective of the present investigation was to study the effect of the flax lignan concentrate (FLC) and Omega-3-fatty acid (O-3-FA) on myocardial apoptosis, left ventricular (LV) contractile dysfunction and electrocardiographic abnormalities in pressure overload-induced cardiac hypertrophy. The rats were divided into five groups such as sham, aortic stenosis (AS), AS+FLC, AS+O-3-FA and AS+FLC+O-3-FA. Cardiac hypertrophy was produced in rats by abdominal aortic constriction. The rats were treated with FLC (400mg/kg, p.o.), O-3-FA (400mg/kg, p.o.) and FLC+O-3-FA orally per day for four weeks. The LV function, myocardial apoptosis, and oxidative stress were quantified. FLC+O-3-FA treatment significantly reduced hemodynamic changes, improved LV contractile dysfunction, reduced cardiomyocyte apoptosis and cellular oxidative stress. Moreover, it significantly up-regulated the VEGF expression and decreased TNF-alpha level in serum. The histological analysis also revealed that FLC+O-3-FA treatment markedly preserved the cardiac structure and inhibited interstitial fibrosis. In conclusion, FLC+O-3-FA treatment improved LV dysfunction, inhibited cardiomyocyte apoptosis, improved myocardial angiogenesis, conserved activities of membrane-bound phosphatase enzymes and suppressed inflammation through reduced oxidative stress in an additive manner than FLC alone and O-3-FA alone treatment in pressure overload-induced cardiac hypertrophy.
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India; Serum Institute of India Ltd., Pune, India.
Arvindkumar E
AE
Ghule
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India.
Amit D
AD
Kandhare
Serum Institute of India Ltd., Pune, India.
Suresh S
SS
Jadhav
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India.
Anand A
AA
Zanwar
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India. Electronic address: drslbodh@gmail.com.
Subhash L
SL
Bodhankar
D000818:Animals; D001024:Aortic Valve Stenosis / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis / Q000187:drug effects*; D002312:Cardiomyopathy, Hypertrophic / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000627:therapeutic use*; D019597:Flax / Q000737:chemistry*; D006439:Hemodynamics / Q000187:drug effects; D017705:Lignans / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D016277:Ventricular Function, Left / Q000187:drug effects
D015525:Fatty Acids, Omega-3; D017705:Lignans; D010936:Plant Extracts
Antioxidants; Apoptosis; Cardiac hypertrophy; Electrocardiography; Flax lignan concentrate; Omega-3-fatty acid; ROS; TNF-α; VEGF