Extent of Detached Retina and Lens Status Influence Intravitreal Protein Expression in Rhegmatogenous Retinal Detachment.
OBJECTIVE
The aim of the study was to compare intravitreal cytokines and chemokines to clinical parameters in patients with rhegmatogenous retinal detachment (RRD).


METHODS
In this prospective study vitreous samples were taken undiluted from 60 patients with RRD and 20 age-matched controls with idiopathic epiretinal membranes at the beginning of primary vitrectomy. The following clinical parameters were assessed from RRD patients prior to surgery: number of quadrants detached, RD height, lens status, symptom duration, and refractive power. Concentrations of 40 different proteins in the vitreous of RRD eyes were measured by multiplex protein array, compared with controls and correlated to clinical parameters.


RESULTS
Ten cytokines and chemokines were significantly upregulated in the vitreous of RRD eyes compared with controls (tissue inhibitors of metalloproteinases [TIMP]-1 and -2, macrophage inflammatory protein [MIP]-1α, monocyte chemoattractant protein [MCP]-1, IL-6, and -8, inducible protein (IP)-10, brain-derived neurotrophic factor [BDNF], TGFβ-3, and platelet-derived growth factor [PDGF]-AB/BB). Linear regression analysis revealed that IL-8 and TGFβ-3 increased with the number of retinal quadrants detached, while TIMP-1 rose in eyes with greater RD heights. Concentrations of IP-10 and myeloperoxidase (MPO) peaked in eyes with two or more quadrants detached, while TIMP-2 was highest expressed in the vitreous of eyes with great RD height. In pseudophakic eyes with higher detachment height levels of vascular cell adhesion molecule (VCAM)-1 were significantly increased, while neural cell adhesion molecule (NCAM) was decreased in pseudophakic patients with shallow RD height.


CONCLUSIONS
Extent of RRD and lens status significantly influence intravitreal proinflammatory, profibrotic, and proapoptotic protein expression. These data contribute to the fundamental understanding of pathophysiological mechanisms in RRD and may serve as a basis for development of adjunct therapeutics to facilitate functional restoration.
Department of Ophthalmology and Optometry Medical University Vienna, Vienna, Austria.
Andreas
A
Pollreisz
Department of Ophthalmology and Optometry Medical University Vienna, Vienna, Austria.
Stefan
S
Sacu
Department of Ophthalmology and Optometry Medical University Vienna, Vienna, Austria.
Katharina
K
Eibenberger
Department of Ophthalmology and Optometry Medical University Vienna, Vienna, Austria.
Marion
M
Funk
Section for Medical Statistics, Medical University Vienna, Vienna, Austria.
Danijel
D
Kivaranovic
Institute of Immunology, Medical University Vienna, Vienna, Austria.
Gerhard J
GJ
Zlabinger
Department of Ophthalmology and Optometry Medical University Vienna, Vienna, Austria.
Michael
M
Georgopoulos
Department of Ophthalmology and Optometry Medical University Vienna, Vienna, Austria.
Ursula
U
Schmidt-Erfurth
D018925:Chemokines / Q000096:biosynthesis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012163:Retinal Detachment / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism*
D018925:Chemokines