Conformational Polymorphism in Autophagy-Related Protein GATE-16.
Autophagy is a fundamental homeostatic process in eukaryotic organisms, fulfilling essential roles in development and adaptation to stress. Among other factors, formation of autophagosomes critically depends on proteins of the Atg8 (autophagy-related protein 8) family, which are reversibly conjugated to membrane lipids. We have applied X-ray crystallography, nuclear magnetic resonance spectroscopy, and molecular dynamics simulations to study the conformational dynamics of Atg8-type proteins, using GATE-16 (Golgi-associated ATPase enhancer of 16 kDa), also known as GABARAPL2, as a model system. This combination of complementary approaches provides new insight into a structural transition centered on the C-terminus, which is crucial for the biological activity of these proteins.
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.|Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf , 40225 Düsseldorf, Germany.
Peixiang
P
Ma
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Oliver
O
Schillinger
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Melanie
M
Schwarten
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Justin
J
Lecher
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Rudolf
R
Hartmann
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.|Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf , 40225 Düsseldorf, Germany.
Matthias
M
Stoldt
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Jeannine
J
Mohrlüder
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Olujide
O
Olubiyi
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Birgit
B
Strodel
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.|Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf , 40225 Düsseldorf, Germany.
Dieter
D
Willbold
Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich , 52425 Jülich, Germany.
Oliver H
OH
Weiergräber
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000737:chemistry*; D001343:Autophagy / Q000502:physiology; D000071190:Autophagy-Related Protein 8 Family; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D048868:Adaptor Proteins, Signal Transducing; D000071190:Autophagy-Related Protein 8 Family; C498698:GABARAPL2 protein, human; D008840:Microfilament Proteins