Lectin-Based Characterization of Vascular Cell Microparticle Glycocalyx.
Microparticles (MPs) are released constitutively and from activated cells. MPs play significant roles in vascular homeostasis, injury, and as biomarkers. The unique glycocalyx on the membrane of cells has frequently been exploited to identify specific cell types, however the glycocalyx of the MPs has yet to be defined. Thus, we sought to determine whether MPs, released both constitutively and during injury, from vascular cells have a glycocalyx matching those of the parental cell type to provide information on MP origin. For these studies we used rat pulmonary microvascular and artery endothelium, pulmonary smooth muscle, and aortic endothelial cells. MPs were collected from healthy or cigarette smoke injured cells and analyzed with a panel of lectins for specific glycocalyx linkages. Intriguingly, we determined that the MPs released either constitutively or stimulated by CSE injury did not express the same glycocalyx of the parent cells. Further, the glycocalyx was not unique to any of the specific cell types studied. These data suggest that MPs from both normal and healthy vascular cells do not share the parental cell glycocalyx makeup.
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America; Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America.
April K
AK
Scruggs
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America; Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America.
Eugene A
EA
Cioffi
Department of Biochemistry, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America; Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America.
Donna L
DL
Cioffi
Department of Pathology, West Virginia University, Morgantown, West Virginia, United States of America.
Judy A C
JA
King
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America; Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama, United States of America.
Natalie N
NN
Bauer
D000818:Animals; D055252:Cell-Derived Microparticles / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D019276:Glycocalyx / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D051381:Rats; D012907:Smoking / Q000009:adverse effects*
D037102:Lectins