[Expressions and co-localization of HIV capsid protein p24 and TRIM22 in HEK293T cells].
OBJECTIVE
To observe the co-localization of human immunodeficiency virus (HIV) capsid protein p24 and tripartite motif containing 22 (TRIM22) in HEK293T cells.


METHODS
The retroviral packaging vector pLP1 was used as the template of p24. After the amplification by PCR, the sequence of p24 was cloned into the eukaryotic expression vector pDsRed-Monomer-N1. The recombinant vector was confirmed by colony PCR, double restriction enzyme digestion and DNA sequencing. HEK293T cells were co-transfected with the vector pDsRed-Monomer-N1-p24 together with pEGFP-N3-TRIM22. After 24 hours, the co-localization of p24-DsRed-Monomer and TRIM22-EGFP was detected under a fluorescence microscope.


RESULTS
Colony PCR, double restriction enzyme digestion and DNA sequencing confirmed that the eukaryotic expression vector pDsRed-Monomer-N1-p24 was constructed successfully. Fluorescence microscope showed that p24-DsRed-Monomer was co-localized with TRIM22-EGFP in HEK293T cells.


CONCLUSIONS
HIV capsid protein p24 is co-localized with TRIM22 in HEK293T cells.
Laboratory of Clinical Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, China.
Dakang
D
Sun
Department of Clinical Laboratory, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
Xinye
X
An
Clinical Laboratory, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, China.
Yuming
Y
Zhou
Department of Clinical Laboratory, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
Bing
B
Ji
Department of Clinical Laboratory, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
Xiangqin
X
Song
Laboratory of Clinical Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, China.
Dianhong
D
Xu
D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D016655:HIV Core Protein p24 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000070584:Tripartite Motif Proteins; D000097573:Red Fluorescent Protein
D036022:Capsid Proteins; D049452:Green Fluorescent Proteins; D016655:HIV Core Protein p24; D008164:Luminescent Proteins; D015778:Minor Histocompatibility Antigens; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D000070584:Tripartite Motif Proteins; D000097573:Red Fluorescent Protein; C094314:TRIM22 protein, human