Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays.
Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms most frequently isolated worldwide and, because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. Previously, P. aeruginosa phages were isolated and best candidates were chosen based on their ability to form clear plaques and their host range. This work aimed to characterize one of those phages, ΦPan70, preliminarily identified as a good candidate for phage-therapy. We performed infection curves, biofilm removal assays, transmission-electron-microscopy, pulsed-field-gel-electrophoresis, and studied the in vivo ΦPan70 biological activity in the burned mouse model. ΦPan70 was classified as a member of the Myoviridae family and, in both planktonic cells and biofilms, was responsible for a significant reduction in the bacterial population. The burned mouse model showed an animal survival between 80% and 100%, significantly different from the control animals (0%). However, analysis of the ΦPan70 genome revealed that it was 64% identical to F10, a temperate P. aeruginosa phage. Gene annotation indicated ΦPan70 as a new, but possible temperate phage, therefore not ideal for phage-therapy. Based on this, we recommend genome sequence analysis as an early step to select candidate phages for potential application in phage-therapy, before entering into a more intensive characterization.
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. av.holguin103@uniandes.edu.co.
Angela V
AV
Holguín
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. garp1@leicester.ac.uk.
Guillermo
G
Rangel
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. vivi.clavijo@gmail.com.
Viviana
V
Clavijo
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. cat-prad@uniandes.edu.co.
Catalina
C
Prada
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. marcelamantilla08@gmail.com.
Marcela
M
Mantilla
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. gomez_katika@hotmail.com.
María Catalina
MC
Gomez
Phage Lab, the Evergreen State College, Olympia, Washington, DC 98505, USA. KutterB@evergreen.edu.
Elizabeth
E
Kutter
Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand. corinda.taylor@otago.ac.nz.
Corinda
C
Taylor
Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand. peter.fineran@otago.ac.nz.
Peter C
PC
Fineran
Grupo de Diseño de Productos y Procesos (GDPP). Department of Chemical Engineering, Universidad de los Andes, Carrera 1E# 19A-40, Bogotá 111711, Colombia. andgonza@uniandes.edu.co.
Andrés Fernando González
AF
Barrios
Department of Biological Sciences, Universidad de los Andes, Carrera 1#18A-12, Bogotá 111711, Colombia. mvives@uniandes.edu.co.
Martha J
MJ
Vives
D000818:Animals; D001433:Bacteriolysis; D018441:Biofilms / Q000254:growth & development*; D002056:Burns / Q000150:complications* / Q000628:therapy; D004195:Disease Models, Animal; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D039002:Prophages / Q000235:genetics*; D011552:Pseudomonas Infections / Q000382:microbiology*; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D016896:Treatment Outcome
Pseudomonas aeruginosa; multiple drug resistance; phage genomics; phage therapy