IMP-51, a novel IMP-type metallo-β-lactamase with increased doripenem- and meropenem-hydrolyzing activities, in a carbapenem-resistant Pseudomonas aeruginosa clinical isolate.
A meropenem-resistant Pseudomonas aeruginosa isolate was obtained from a patient in a medical setting in Hanoi, Vietnam. The isolate was found to have a novel IMP-type metallo-β-lactamase, IMP-51, which differed from IMP-7 by an amino acid substitution (Ser262Gly). Escherichia coli expressing blaIMP-51 showed greater resistance to cefoxitin, meropenem, and moxalactam than E. coli expressing blaIMP-7. The amino acid residue at position 262 was located near the active site, proximal to the H263 Zn(II) ligand.
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Tatsuya
T
Tada
Department of Microbiology, Hanoi Medical University, Hanoi, Vietnam Bach Mai Hospital, Hanoi, Vietnam.
Pham Hong
PH
Nhung
Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Tohru
T
Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Kayo
K
Shimada
Bach Mai Hospital, Hanoi, Vietnam.
Doan Mai
DM
Phuong
Bach Mai Hospital, Hanoi, Vietnam.
Nguyen Quoc
NQ
Anh
Disease Control and Prevention Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Norio
N
Ohmagari
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan tkirikae@ri.ncgm.go.jp.
Teruo
T
Kirikae
D015780:Carbapenems / Q000494:pharmacology*; D002440:Cefoxitin / Q000494:pharmacology; D000077726:Doripenem; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D009070:Moxalactam / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology; D013845:Thienamycins / Q000494:pharmacology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D015780:Carbapenems; D013845:Thienamycins; D002440:Cefoxitin; D000077726:Doripenem; D001618:beta-Lactamases; D000077731:Meropenem; D009070:Moxalactam