Changes in expression of insulin signaling pathway genes by dietary fat source in growing-finishing pigs.
This study investigated changes in gene expression by dietary fat source, i.e., beef tallow, soybean oil, olive oil, and coconut oil (each 3% in feed), in both male and female growing-finishing pigs. Real-time PCR was conducted on seven genes (insulin receptor; INSR, insulin receptor substrate; IRS, phosphatidylinositol (3,4,5)-triphosphate; PIP3, 3-phosphoinositide-dependent protein kinase-1; PDK1, protein kinase B; Akt, forkhead box protein O1; FOXO1 and cGMP-inhibited 3', 5'-cyclic phosphodiesterase; PDE3) located upstream of the insulin signaling pathway in the longissimus dorsi muscle (LM) of pigs. The INSR, IRS, PIP3, and PDE3 genes showed significantly differential expression in barrow pigs. Expression of the PIP3 and FOXO1 genes was significantly different among the four dietary groups in gilt pigs. In particular, the PIP3 gene showed the opposite expression pattern between barrow and gilt pigs. These results show that dietary fat source affected patterns of gene expression according to animal gender. Further, the results indicate that the type of dietary fat affects insulin signaling-related gene expression in the LM of pigs. These results can be applied to livestock production by promoting the use of discriminatory feed supplies.
Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Chuksan-gil 77, Kwonsun-gu, Suwon, Korea.
Seung-Chang
SC
Kim
Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Chuksan-gil 77, Kwonsun-gu, Suwon, Korea.
Hong-Chul
HC
Jang
Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheon-an, Chungnam, 330-801 Korea.
Sung-Dae
SD
Lee
Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheon-an, Chungnam, 330-801 Korea.
Hyun-Jung
HJ
Jung
Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheon-an, Chungnam, 330-801 Korea.
Jun-Cheol
JC
Park
Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Chuksan-gil 77, Kwonsun-gu, Suwon, Korea.
Seung-Hwan
SH
Lee
Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Chuksan-gil 77, Kwonsun-gu, Suwon, Korea.
Tae-Hun
TH
Kim
Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Chuksan-gil 77, Kwonsun-gu, Suwon, Korea.
Bong-Hwan
BH
Choi
Dietary fat; Gene expression; Growing-finishing pig; Insulin signaling pathway