Short communication: Proteomic characterization of tuberculin purified protein derivative from Mycobacterium bovis.
Bovine tuberculin purified protein derivative (bPPD) is used as an intradermal test (IT) reagent to detect bovine tuberculosis (bTB) in most countries. Identification of bPPD proteins is critical to understanding the immunological reaction of IT at the molecular level. While bPPD from the United Kingdom (UK) and Brazil (BR) have been recently defined at the proteomic level, bPPD from the Republic of Korea (KR) has not yet been analyzed. Here, bPPD KR proteome was examined for the first time. In total, 271 proteins were identified, including Mycobacterium bovis-specific proteins Mb0854c and Mb2898, and 42 known T cell antigens. On comparing with proteomes of bPPD UK and BR, 33 proteins were found to be common among all three bPPDs, of which 15 proteins were T cell antigens. M. bovis-specific antigens with T cell activity in bPPD may be novel candidates for use as alternatives to currently available bPPD in diagnostics.
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea. Electronic address: choys@korea.kr.
Yun Sang
YS
Cho
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Young-Boo
YB
Jang
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Sang-Eun
SE
Lee
Laboratory of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Je-Yoel
JY
Cho
Laboratory of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Jung-Mo
JM
Ahn
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Inyeong
I
Hwang
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Eunjeong
E
Heo
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Hyang-Mi
HM
Nam
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Donghee
D
Cho
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Moon
M
Her
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Young Hwa
YH
Jean
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Suk Chan
SC
Jung
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Jong Man
JM
Kim
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Hee Soo
HS
Lee
Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Keechan
K
Lee
Mycobacterial Research Laboratories Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
John T
JT
Belisle
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D009163:Mycobacterium bovis / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D056910:Republic of Korea; D014373:Tuberculin / Q000235:genetics*; D014374:Tuberculin Test / Q000379:methods / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000382:microbiology
D001426:Bacterial Proteins; D014373:Tuberculin
Bovine tuberculosis; Mycobacterium bovis; Proteomic analysis; Purified protein derivative tuberculin; T cell antigen