Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model.
Extensive urethral defects have a serious impact on quality of life, and treatment is challenging. A shortage of material for reconstruction is a key limitation. Improving the properties of biomaterials and making them suitable for urethral reconstruction will be helpful. Previously, we constructed a fusion protein, collagen-binding VEGF (CBD-VEGF), which can bind to collagen scaffold, stimulate cell proliferation, and promote angiogenesis and tissue regeneration. We proposed that CBD-VEGF could improve the performance of collagen in reconstruction of extensive urethral defects. Our results showed that collagen scaffolds modified with CBD-VEGF could promote urethral tissue regeneration and improve the function of the neo-urethra in a beagle extensive urethral defect model. Thus, modifying biomaterials with bioactive factors provides an alternative strategy for the production of suitable biomaterials for urethral reconstruction.
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Weisheng
W
Jia
Department of Chemical-defence Medicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
He
H
Tang
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Jianjian
J
Wu
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
Xianglin
X
Hou
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
Bing
B
Chen
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Wei
W
Chen
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
Yannan
Y
Zhao
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
Chunying
C
Shi
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Feng
F
Zhou
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Wei
W
Yu
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Shengquan
S
Huang
Department of Urology, Center of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address: gangyeah@126.com.
Gang
G
Ye
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jwdai@genetics.ac.cn.
Jianwu
J
Dai
D000818:Animals; D001665:Binding Sites; D003094:Collagen / Q000737:chemistry*; D004285:Dogs; D008297:Male; D011788:Quality of Life; D019651:Plastic Surgery Procedures; D012038:Regeneration; D054457:Tissue Scaffolds / Q000737:chemistry*; D014521:Urethra / Q000187:drug effects / Q000293:injuries* / Q000502:physiology* / Q000601:surgery; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000627:therapeutic use*
D042461:Vascular Endothelial Growth Factor A; D003094:Collagen
Collagen; Collagen-binding domain; Urethral regeneration; Vascular endothelial growth factors