Bioprecipitation of Calcium Carbonate Crystals by Bacteria Isolated from Saline Environments Grown in Culture Media Amended with Seawater and Real Brine.
The precipitation of calcium carbonate and calcium sulphate by isolated bacteria from seawater and real brine obtained in a desalination plant growth in culture media containing seawater and brine as mineral sources has been studied. However, only bioprecipitation was detected when the bacteria were grown in media with added organic matter. Biomineralization process started rapidly, crystal formation taking place in the beginning a few days after inoculation of media; roughly 90% of total cultivated bacteria showed. Six major colonies with carbonate precipitation capacity dominated bacterial community structure cultivated in heterotrophic platable bacteria medium. Taxonomic identification of these six strains through partial 16S rRNA gene sequences showed their affiliation with Gram-positive Bacillus and Virgibacillus genera. These strains were able to form calcium carbonate minerals, which precipitated as calcite and aragonite crystals and showed bacterial fingerprints or bacteria calcification. Also, carbonic anhydrase activity was observed in three of these isolated bacteria. The results of this research suggest that microbiota isolated from sea water and brine is capable of precipitation of carbonate biominerals, which can occur in situ with mediation of organic matter concentrations. Moreover, calcium carbonate precipitation ability of this microbiota could be of importance in bioremediation of CO2 and calcium in certain environments.
Department of Microbiology, Faculty of Farmacy, University of Granada, University Campus of Cartuja, 18071 Granada, Spain.
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Silva-Castro
Department of Microbiology, Faculty of Farmacy, University of Granada, University Campus of Cartuja, 18071 Granada, Spain.
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Uad
Department Civil Engineering, School of Civil Engineering, University of Granada, Campus of Fuentenueva, 18071 Granada, Spain.
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Gonzalez-Martinez
Department of Electronic and Computer Technology, School of Computer Sciences and Telecomunications, University of Granada, University Campus of Almanjayar, 18071 Granada, Spain.
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Rivadeneyra
Department of Microbiology, Faculty of Farmacy, University of Granada, University Campus of Cartuja, 18071 Granada, Spain.
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Gonzalez-Lopez
Department of Microbiology, Faculty of Farmacy, University of Granada, University Campus of Cartuja, 18071 Granada, Spain.
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Rivadeneyra
D002119:Calcium Carbonate / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002245:Carbon Dioxide / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011232:Chemical Precipitation; D003460:Crystallization; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D012492:Salts / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*
D003470:Culture Media; D012492:Salts; C017082:brine; D002245:Carbon Dioxide; D002119:Calcium Carbonate