Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes.
BACKGROUND
Trichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Trichinella infection and immunodiagnosis.


RESULTS
The T. spiralis adult cDNA library was immunoscreened with sera from pigs experimentally infected with 20,000 infective T. spiralis larvae. Total 43 positive clones encoding for 28 proteins were identified; one of the immunodominant proteins was 20 kDa Ts-ES-1 secreted by Trichinella stichocytes and existing in the excretory/secretory (ES) products of T. spiralis adult and muscle larval worms. Ts-ES-1 contains 172 amino acids with a typical signal peptide in the first 20 amino acids. The expression of Ts-ES-1 was detected in both the adult and muscle larval stages at the mRNA and protein expression levels. Mice immunized with recombinant Ts-ES-1 (rTs-ES-1) formulated with ISA50v2 adjuvant exhibited a significant worm reduction in both the adult worm (27%) and muscle larvae burden (42.1%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rTs-ES-1-induced protection was associated with a high level of specific anti-Ts-ES-1 IgG antibodies and a Th1/Th2 mixed immune response.


CONCLUSIONS
The newly identified rTs-ES-1 is an immunodominant protein secreted by Trichinella stichocytes during natural infection and enables to the induction of partial protective immunity in vaccinated mice against Trichinella infection. Therefore, rTs-ES-1 is a potential candidate for vaccine development against trichinellosis.
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
Kuo
K
Bi
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
Jing
J
Yang
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China; Beijing Friendship Hospital, Capital Medical University, Beijing, PR China.
Lei
L
Wang
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
Yuan
Y
Gu
Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America.
Bin
B
Zhan
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
Xinping
X
Zhu
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics; D005260:Female; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000235:genetics; D007814:Larva / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D016056:Immunodominant Epitopes; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic