Myroides indicus sp. nov., isolated from garden soil.
A novel aerobic, non-motile, rod-shaped, catalase- and oxidase-positive bacterial strain, designated UKS3T, was isolated from garden soil, and subjected to polyphasic taxonomic analysis. Strain UKS3T formed whitish, viscous colonies on nutrient agar and was Gram-staining negative. Phylogenetic analysis, based on 16S rRNA gene sequence, showed that maximum pairwise similarity occurs with representatives of the genus Myroides. The most closely related species include Myroides marinus JS-08T (92.7 % sequence similarity), Myroides phaeus MY15T (92.7 %), Myroides odoratus DSM 2801T (91.5 %) and Myroides odoratimimus CCUG 39352T (91.4 %). Strain UKS3T contained menaquinone-6 (MK-6) as the major respiratory quinone and iso-C15 : 0 (40.2 %), anteiso-C15 : 0 (9.4 %) and iso-C17 : 0 3-OH (8.5 %) as major fatty acids. Phosphatidylethanolamine, phospholipids and three aminolipids were the major polar lipids. The DNA G+C content of strain UKS3T was 36.8 ± 2.0 mol%. On the basis of phenotypic, chemotaxonomic and molecular analysis, strain UKS3T represents a novel species of the genus Myroides, for which the name Myroides indicus sp. nov., is proposed. The type strain is UKS3T ( = DSM 28213T = NCIM 5555T ).
Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi -110 007, India.
Hari
H
Ram
Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi -110 007, India.
Alok
A
Kumar
Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi -110 007, India.
Lebin
L
Thomas
NCIM Resource Center CSIR, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune -411008, Maharashtra, India.
Syed G
SG
Dastager
NCIM Resource Center CSIR, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune -411008, Maharashtra, India.
Rahul
R
Mawlankar
Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi -110 007, India.
Ved Pal
VP
Singh
D001420:Bacteria, Aerobic / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000072506:Gardens; D007194:India; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6