Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates.
A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (kcat/Km) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3-825.6 mg L(-1)) by the evolved mutants was 42-60-fold faster than that by the wild type.
Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Republic of Korea. metalkim@kw.ac.kr.
Quang Anh Tuan
QA
Le
Rakwoo
R
Chang
Yong Hwan
YH
Kim
D043303:Aryldialkylphosphatase / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D006868:Hydrolysis; D016297:Mutagenesis, Site-Directed; D009943:Organophosphorus Compounds / Q000737:chemistry*
D009943:Organophosphorus Compounds; D043303:Aryldialkylphosphatase