Convoluted Plasma Membrane Domains in the Green Alga Chara are Depleted of Microtubules and Actin Filaments.
Charasomes are convoluted plasma membrane domains in the green alga Chara australis. They harbor H(+)-ATPases involved in acidification of the medium, which facilitates carbon uptake required for photosynthesis. In this study we investigated the distribution of cortical microtubules and cortical actin filaments in relation to the distribution of charasomes. We found that microtubules and actin filaments were largely lacking beneath the charasomes, suggesting the absence of nucleating and/or anchoring complexes or an inhibitory effect on polymerization. We also investigated the influence of cytoskeleton inhibitors on the light-dependent growth and the darkness-induced degradation of charasomes. Inhibition of cytoplasmic streaming by cytochalasin D significantly inhibited charasome growth and delayed charasome degradation, whereas depolymerization of microtubules by oryzalin or stabilization of microtubules by paclitaxel had no effect. Our data indicate that the membrane at the cytoplasmic surface of charasomes has different properties in comparison with the smooth plasma membrane. We show further that the actin cytoskeleton is necessary for charasome growth and facilitates charasome degradation presumably via trafficking of secretory and endocytic vesicles, respectively. However, microtubules are required neither for charasome growth nor for charasome degradation.
Department of Cell Biology, Division of Plant Physiology, University of Salzburg, 5020 Salzburg, Austria.
Aniela
A
Sommer
Department of Cell Biology, Division of Plant Physiology, University of Salzburg, 5020 Salzburg, Austria.
Margit
M
Hoeftberger
Department of Cell Biology, Division of Plant Physiology, University of Salzburg, 5020 Salzburg, Austria.
Marion C
MC
Hoepflinger
Department of Cell Biology, Division of Plant Physiology, University of Salzburg, 5020 Salzburg, Austria.
Sarah
S
Schmalbrock
Department of Biophysics, Faculty of Biology, Moscow State University, Moscow 119991, Russia.
Alexander
A
Bulychev
Department of Cell Biology, Division of Plant Physiology, University of Salzburg, 5020 Salzburg, Austria ilse.foissner@sbg.ac.at.
Ilse
I
Foissner
D008841:Actin Cytoskeleton / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D044784:Chara / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*
Chara australis; Charasome; Cytoskeleton; Internodal cell; Plant; pH banding