Comparison of systemic inflammation response and vital organ damage induced by severe burns in different area.
BACKGROUND
In this study, we will establish a stable and optimized rat model that can meet strictly diagnosed criteria and serve as a tool to investigate the potential of novel therapeutics in this preclinical model through comparative analysis of systemic alterations, levels of pro-inflammatory cytokines in serum and infiltrated numbers of inflammatory cells in distant organ between 30% and 50% TBSA with a full-thickness burn.


METHODS
The adult male Wistar rats were randomly divided into the following groups: control group, 30% TBSA with a full-thickness burn group, and 50% TBSA with a full-thickness burn group. The blood and serum samples in the 3 groups were collected and detected by blood routine examination and biochemical detection at 6 h, 12 h, 24 h and 48 h post burn. The levels of TNF-α, IL-1β and IL-6 in serum were detected by ELISA. The sections of lung, renal, liver and heart were analyzed by H&E and immunohistochemical staining detection.


RESULTS
Our results showed that temperature in 50% TBSA with a full-thickness burn group was always hypothermia, and lower than 36°C at defined timepoints post burn, that was in 30% TBSA with a full-thickness burn group was lower than 36°C only at 48 h post burn. The levels of TNF-α, IL-1β and IL-6 were significantly increased in 30% and 50% groups at 6 h, 12 h, 24 h and 48 h post burn. The apoptosis in distant organs and the biochemical parameters such as ALT, AST, troponin, CK, CK-MB, LDH, urea and creatinine in 30% and 50% groups were also increased at different degrees at defined timepoints after burn, but changes in 50% group were more obvious than that in 30% group.


CONCLUSIONS
We choose 50% TBSA with a full-thickness burn to establish a stable and optimized rat model that can meet strictly diagnosed criteria and serve as a tool to investigate the potential of novel therapeutics in this preclinical model.
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Lingying
L
Liu
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Xiao
X
Li
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Jing
J
Yang
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Jiake
J
Chai
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Yonghui
Y
Yu
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Hongjie
H
Duan
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Huifeng
H
Song
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Rui
R
Feng
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Tongming
T
Wang
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Huinan
H
Yin
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Quan
Q
Hu
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Shaoxia
S
Wang
Department of Burn & Plastic Surgery, Burn Institute, The First Affiliated Hospital of PLA General Hospital Haidian District, Beijing 100048, China.
Jundong
J
Du
D000818:Animals; D002056:Burns / Q000473:pathology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation / Q000209:etiology / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
Animal model; excessively inflammatory response; severe burn; systemic changes