[Chemical constituents from culture of Streptomyces sp. CPCC 202950].
Eleven compounds were isolated from the culture of Streptomyces sp. CPCC 202950 by a combination of various chromatographic techniques including column chromatography over macroporous resin HP-20, MCI, and reversed-phase HPLC. Their structures were identified as 1H-pyrrole-2-carboxamide(1),5'-deoxy-5'-methylthioinosine(2), vanillamide(3), trans-3-methylthioacrylamide(4), 1,2,3,4-Tetraydro-1H-pyrido[3,4-b]indole-3-carboxylic acid(5), cyclo(L-pro-L-tyr) (6), N-[2-(4-hydroxyphenyl)]ethylacetamide(7), benzamide (8), cyclo ('L-leucyl-trans-4-hydroxy-L-proline)(9), cyclo-(Phe-Gly) (10), and tryptophan (11). Among them, compounds 1 and 2 were new natural products. In the preliminary assays, none of the compounds exhibited obvious inhibition of HIV-1 protease activity (IC50 > 10 micromol x L(-1)).
Ming-hua
MH
Chen
Ye-xiang
YX
Wu
Biao
B
Dong
Xiu-yong
XY
Fan
Li-yan
LY
Yu
Wei
W
Jiang
Shu-yi
SY
Si
D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D016333:HIV Protease / Q000032:analysis; D017320:HIV Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
D003470:Culture Media; D017320:HIV Protease Inhibitors; D016333:HIV Protease