Inflation with carbon monoxide in rat donor lung during cold ischemia phase ameliorates graft injury.
Carbon monoxide (CO) attenuates lung ischemia reperfusion injury (IRI) via inhalation, and as an additive dissolved in flush/preservation solution. This study observed the effects of lung inflation with CO on lung graft function in the setting of cold ischemia. Donor lungs were inflated with 40% oxygen + 60% nitrogen (control group) or with 500 ppm CO + 40% oxygen + nitrogen (CO group) during the cold ischemia phase and were kept at 4℃ for 180 min. Recipients were sacrificed by exsanguinations at 180 min after reperfusion. Rats in the sham group had no transplantation and were performed as the recipients. Compared with the sham group, the oxygenation determined by blood gas analysis and the pressure-volume curves of the lung grafts decreased significantly, while the wet weight/dry weight (W/D) ratio, inflammatory reaction, oxidative stress, and cell apoptosis increased markedly (P < 0.05). However, compared to the control group, CO treatment improved the oxygenation (381 ± 58 vs. 308 ± 78 mm Hg) and the pressure-volume curves (15.8 ± 2.4 vs. 11.6 ± 1.7 mL/kg) (P < 0.05). The W/D ratio (4.6 ± 0.6) and the serum levels of interleukin-8 (279 ± 46 pg/mL) and tumor necrosis factor-α (377 ± 59 pg/mL) in the CO group decreased significantly compared to the control group (5.8 ± 0.8, 456 ± 63 pg/mL, and 520 ± 91 pg/mL) (P < 0.05). In addition, CO inflation also significantly decreased malondialdehyde activity and apoptotic cells in grafts, and increased the superoxide dismutase content. Briefly, CO inflation in donor lungs in the setting of cold ischemia attenuated lung IRI and improved the graft function compared with oxygen.
Department of Anesthesiology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China; 150001, China Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150001, China.
Chao
C
Meng
Department of Dermatology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
Liangjuan
L
Ma
Department of Anesthesiology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China; 150001, China Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150001, China.
Jinfeng
J
Liu
Department of Anesthesiology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China; 150001, China Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150001, China.
Xiaoguang
X
Cui
Department of Anesthesiology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China; 150001, China Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150001, China.
Rongfang
R
Liu
Department of Anesthesiology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China; 150001, China Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150001, China.
Jingchun
J
Xing
Department of Anesthesiology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150001, China; 150001, China Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150001, China Department of Anesthesiology, the Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China zhouhuacheng@163.com.
Huacheng
H
Zhou
D000818:Animals; D001784:Blood Gas Analysis; D002248:Carbon Monoxide / Q000008:administration & dosage* / Q000494:pharmacology; D050377:Cold Ischemia / Q000009:adverse effects*; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D010100:Oxygen / Q000097:blood; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D019737:Transplants / Q000187:drug effects*; D016896:Treatment Outcome
D002248:Carbon Monoxide; D010100:Oxygen
Carbon monoxide; cold ischemia phase; ischemia reperfusion injury; lung inflation; lung transplantation