The Fecal Virome of Children with Hand, Foot, and Mouth Disease that Tested PCR Negative for Pathogenic Enteroviruses.
Hand, foot, and mouth disease (HFMD) affects infant and young children. A viral metagenomic approach was used to identify the eukaryotic viruses in fecal samples from 29 Thai children with clinical diagnosis of HFMD collected during the 2012 outbreak. These children had previously tested negative by PCR for enterovirus 71 and coxsackievirus A16 and A6. Deep sequencing revealed nine virus families: Picornaviridae, Astroviridae, Parvoviridae, Caliciviridae, Paramyxoviridae, Adenoviridae, Reoviridae, Picobirnaviridae, and Polyomaviridae. The highest number of viral sequences belonged to human rhinovirus C, astrovirus-MLB2, and coxsackievirus A21. Our study provides an overview of virus community and highlights a broad diversity of viruses found in feces from children with HFMD.
Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, Thailand.
Piyada
P
Linsuwanon
Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, Thailand.
Yong
Y
Poovorawan
Blood Systems Research Institute, San Francisco, California, United States of America; Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.
Linlin
L
Li
Blood Systems Research Institute, San Francisco, California, United States of America; Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.
Xutao
X
Deng
Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, Thailand.
Sompong
S
Vongpunsawad
Blood Systems Research Institute, San Francisco, California, United States of America; Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.
Eric
E
Delwart
D001483:Base Sequence; D002648:Child; D004279:DNA, Viral / Q000235:genetics; D004770:Enterovirus / Q000235:genetics; D005243:Feces / Q000821:virology*; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D013785:Thailand
D004279:DNA, Viral; D012367:RNA, Viral