LncRNA-AP001631.9 promotes cell migration in gastric cancer.
Recently, an increasing number of reports have revealed that long non-coding RNAs (LncRNAs) play important roles in a variety of aspects of cell activity, and their aberrant expression is closely associated with multigenetic diseases, including carcinoma. In the present study, through microarray analysis, we screened out a new LncRNA (LncRNA-AP001631.9), which was regulated by FOXM1, a well-known carcinogenetic factor and aimed to reveal the functional roles of this novel LncRNA in gastric cancer development. The data from qRT-PCR confirmed that the expression level of LncRNA-AP001631.9 was positively correlated with that of FOXM1. The transwell and wound healing assays indicated that LncRNA-AP001631.9 was required for the migration of gastric cancer cells. The downregulation of LncRNA-AP001631.9 by small interference RNA suppressed the migratory ability of MGC803 and AGS cells, while the overexpression of LncRNA-AP001631.9 promoted the movement of BGC823 and SGC7901 cells. Furthermore, a tail vein injection was administered, and the obtained results suggested that LncRNA-AP001631.9 contributed to the distant metastasis of SGC7901 cells. Moreover, we also collected 36 paired samples of gastric cancer tissues to explore the expression levels of LncRNA-AP001631.9. The qRT-PCR data indicated that the LncRNA-AP001631.9 expression was frequently increased in gastric cancer tissues. Taken together, our findings established that LncRNA-AP001631.9 plays critical roles in gastric cancer progression and can serve as a potential new target for the treatment of gastric cancer.
Department of Oncology, East Hospital, Tongji University School of Medicine 150 Ji-Mo Road, Shanghai 200120, China.
Hui
H
Cai
Department of Oncology, East Hospital, Tongji University School of Medicine 150 Ji-Mo Road, Shanghai 200120, China.
Xiaojuan
X
Ye
Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine 150 Ji-Mo Road, Shanghai 200120, China.
Bin
B
He
Department of Oncology, East Hospital, Tongji University School of Medicine 150 Ji-Mo Road, Shanghai 200120, China.
Qiang
Q
Li
Department of Oncology, East Hospital, Tongji University School of Medicine 150 Ji-Mo Road, Shanghai 200120, China.
Yandong
Y
Li
Department of Oncology, East Hospital, Tongji University School of Medicine 150 Ji-Mo Road, Shanghai 200120, China.
Yong
Y
Gao
D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014162:Transfection
D014408:Biomarkers, Tumor; C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering
FoxM1; LncRNA; cell migration; gastric cancer