[Synthesis, identification of artificial antigen of catalpol and preliminary study of immunogenicity].
The method of monoclonal antibody-based immunoassay has a great importance in the study of quality control of traditional Chinese medicine (TCM) and detection of trace components in vivo animals. Synthesis of small molecule artificial antigen is the prerequisite for the establishment of this method. In present study, catalpol-BSA was synthesized by sodium periodate oxidation method. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry ( MALDI-TOF-MS) and molecular exclusion chromatography showed that catalpol was successfully conjugated with BSA. The mice could specifically produce anti-catalpol antibodies with titer up to 1:8000. The artificial antigen of catalpol was successfully synthesized.
Zhuang
Z
Li
Wei-ku
WK
Zhang
Yue
Y
Zhang
Geng-ni
GN
Di
Hui-hua
HH
Qu
Yan
Y
Zhao
Qing-guo
QG
Wang
Jie-kun
JK
Xu
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D007118:Immunoassay; D057888:Iridoid Glucosides / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D057888:Iridoid Glucosides; C078040:catalpol; D012710:Serum Albumin, Bovine