Novel relationships between B12, folate and markers of inflammation, oxidative stress and NAD(H) levels, systemically and in the CNS of a healthy human cohort.
OBJECTIVE
To evaluate the relationship between folate, cobalamin (Cbl), and homocysteine (Hcy), and markers of inflammation and oxidative stress within the periphery and central nervous system (CNS) of a healthy human cohort.


METHODS
Thirty-five matched cerebrospinal fluid (CSF) and plasma samples were collected from consenting participants who required a spinal tap for the administration of anaesthetic. Plasma concentrations of Hcy and both plasma and CSF levels of folate, Cbl, nicotinamide adenine dinucleotide (NAD(H)) and markers of inflammation (interleukin-6, IL-6), and oxidative stress (F2-isoprostanes, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and total antioxidant capacity (TAC)) were quantified.


RESULTS
In the peripheral circulation, positive associations were observed between plasma folate and Cbl, and plasma TAC (P ≤ 0.01; P ≤ 0.01) and plasma NAD(H) (P ≤ 0.05; P ≤ 0.05) levels, respectively. Plasma folate was inversely associated with plasma Hcy concentrations (P ≤ 0.05); however, no statistically significant relationships were observed between plasma Hcy and plasma markers of inflammation, oxidative stress, or [NAD(H)]. Within the CNS plasma Hcy correlated positively with CSF IL-6 (P ≤ 0.01) and negatively with CSF NAD(H) (P ≤ 0.05) concentrations. An inverse association was observed between CSF folate and CSF levels of IL-6 (P ≤ 0.05). Unexpectedly, a positive association between CSF Cbl and CSF 8-OHdG levels was also found (P ≤ 0.01).


CONCLUSIONS
These results indicate that folate and Cbl concentrations may influence the levels of oxidative damage, inflammation, and NAD(H), both systemically and within the CNS.
Jade
J
Guest
Ayse
A
Bilgin
Bevan
B
Hokin
Trevor A
TA
Mori
Kevin D
KD
Croft
Ross
R
Grant
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood / Q000134:cerebrospinal fluid; D028441:F2-Isoprostanes / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage* / Q000097:blood / Q000134:cerebrospinal fluid; D006710:Homocysteine / Q000097:blood / Q000134:cerebrospinal fluid; D006801:Humans; D007249:Inflammation / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood / Q000134:cerebrospinal fluid; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009243:NAD / Q000097:blood* / Q000134:cerebrospinal fluid*; D018384:Oxidative Stress; D014805:Vitamin B 12 / Q000008:administration & dosage* / Q000097:blood / Q000134:cerebrospinal fluid
D000975:Antioxidants; D015415:Biomarkers; D028441:F2-Isoprostanes; D015850:Interleukin-6; D006710:Homocysteine; D009243:NAD; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005492:Folic Acid; D003849:Deoxyguanosine; D014805:Vitamin B 12
Brain; Central nervous system; Cobalamin; Folate; Homocysteine; Inflammation; Nicotinamide adenine dinucleotide; Oxidative stress