Molecular characterization and first report of Cryptosporidium genotypes in human population in the Slovak Republic.
In our study, we examined 91 fecal samples from five different groups of people containing HIV patients, hemodialysis patients, kidney transplant recipients, immunocompetent humans without clinical signs, and humans with suspected cryptosporidiosis. The purpose of our study was to determine species and genotype composition of representatives of Cryptosporidium spp. using PCR analysis of small subunit ribosomal RNA gene and 60-kDa glycoprotein gene and examine their phylogenetic relationship. In HIV-positive/AIDS-infected group of patients and in hemodialysis patients, no presence of Cryptosporidium species was detected. In two kidney transplant recipients, we detected species/genotypes Cryptosporidium parvum IIaA13G1T1R1 (KT355488) and Cryptosporidium hominis IaA11G2R8 (KT355489) and in two immunocompetent patients with clinical symptoms, we identified Cryptosporidium muris and C. hominis IbA10G2T1 (KT355490). In the group of healthy immunocompetent individuals without clinical signs, we identified species/genotype C. hominis IbA11G2 (KT355491) in one sample.
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
Antónia
A
Petrincová
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
Alexandra
A
Valenčáková
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
Lenka
L
Luptáková
HPL, spol. s r. o, Department of Parasitology, Devinská Nová Ves, Bratislava, Slovak Republic.
František
F
Ondriska
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
Jana
J
Kalinová
Department of Public Health, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovak Republic.
Monika
M
Halánová
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
Oľga
O
Danišová
Department of Infectology and Travel Medicine, Pavol Jozef Šafárik University, Faculty of Medicine, Košice, Slovak Republic.
Pavol
P
Jarčuška
D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005243:Feces / Q000469:parasitology; D015658:HIV Infections / Q000469:parasitology; D006801:Humans; D016867:Immunocompromised Host; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D018154:Slovakia / Q000453:epidemiology
D012335:RNA, Ribosomal
Cryptosporidium infection; Immunocompetent humans; Immunocompromised humans; Nested PCR; Primers