[Relationship between vitamin D receptor gene polymorphisms and chronic periodontitis].
OBJECTIVE
To investigate the relationship between vitamin D receptor (VDR) gene polymorphisms and chronic periodontitis(CP).


METHODS
Buccal swabs from 105 patients with mild/mode-rate CP and 85 severe CP were collected, DNA was extracted from these buccal swabs using the TIANamp Swab DNA Kit [TIANGEN Biotech (Beijing) CO.Ltd]. The VDR rs1544410 and rs731236 were genotyped by the Sequenom MassARRAY system (Shanghai Benegene Biotechnology Co. Ltd), which was based on MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) technology. The distribution of the genotypes and allele frequencies were analyzed.


RESULTS
The frequencies of the rs1544410 A allele and AA+AG genotype were significantly higher in severe CP than in mild/moderate CP of all the patients and the female patients respectively (all the patients: P=0.006, 0.007; the female patients: P=0.001, 0.001). The frequencies of the rs731236 C allele and CC+CT genotype were significantly higher in severe CP than in mild/moderate CP of all the patients and the female patients respectively (all the patients: P=0.003, 0.004; the female patients: P<0.001, <0.001).


CONCLUSIONS
Gene polymorphisms of VDR rs731236 and rs1544410 may be associated with severe CP in Chinese Han population.
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Xiao-jing
XJ
Cao
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Lu
L
He
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Huan-xin
HX
Meng
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Peng
P
Li
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Zhi-bin
ZB
Chen
D000483:Alleles; D002681:China; D055113:Chronic Periodontitis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D018167:Receptors, Calcitriol / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018167:Receptors, Calcitriol