Protective properties of ginsenoside Rb3 against UV-B radiation-induced oxidative stress in HaCaT keratinocytes.
This work aimed to evaluate the skin anti-photoaging properties of ginsenoside Rb3 (Rb3), one of the main protopanaxdiol-type ginsenosides from ginseng, in HaCaT keratinocytes. The skin anti-photoaging activity was assessed by analyzing the levels of reactive oxygen species (ROS), pro-matrix metalloproteinase-2 (proMMP-2), pro-matrix metalloproteinase-9 (proMMP-9), total glutathione (GSH), and superoxide dismutase (SOD) activity as well as cell viability in HaCaT keratinocytes under UV-B irradiation. When HaCaT keratinocytes were exposed to Rb3 prior to UV-B irradiation, Rb3 exhibited suppressive activities on UV-B-induced ROS, proMMP-2, and proMMP-9 enhancements. On the contrary, Rb3 displayed enhancing activities on UV-B-reduced total GSH and SOD activity levels. Rb3 could not interfere with cell viabilities in UV-B-irradiated HaCaT keratinocytes. Rb3 plays a protective role against UV-B-induced oxidative stress in human HaCaT keratinocytes, proposing its potential skin anti-photoaging properties.
a Department of Biological Sciences , Kangwon National University , Chuncheon , Republic of Korea.
Sun-Joo
SJ
Oh
a Department of Biological Sciences , Kangwon National University , Chuncheon , Republic of Korea.
Yuri
Y
Oh
a Department of Biological Sciences , Kangwon National University , Chuncheon , Republic of Korea.
In Wang
IW
Ryu
a Department of Biological Sciences , Kangwon National University , Chuncheon , Republic of Korea.
Kyunghoon
K
Kim
b Department of Biochemistry , College of Natural Sciences, Kangwon National University , Chuncheon , Republic of Korea.
Chang-Jin
CJ
Lim
D002461:Cell Line, Transformed; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D036145:Ginsenosides / Q000494:pharmacology*; D005978:Glutathione / Q000819:agonists / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D004792:Enzyme Precursors; D036145:Ginsenosides; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; C044463:ginsenoside Rb3; D013482:Superoxide Dismutase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D005978:Glutathione
UV-B radiation; ginsenoside Rb3; glutathione; oxidative stress; reactive oxygen species