Human RNA polymerase II associated factor 1 complex promotes tumorigenesis by activating c-MYC transcription in non-small cell lung cancer.
Human RNA polymerase II (RNAPII)-associated factor 1 complex (hPAF1C) plays a crucial role in protein-coding gene transcription. Overexpression of hPAF1C has been implicated in the initiation and progression of various human cancers. However, the molecular pathways involved in tumorigenesis through hPAF1C remain to be elucidated. The current study suggested hPAF1C expression as a prognostic biomarker for early stage non-small cell lung cancer (NSCLC) and patients with low hPAF1C expression levels had significantly better overall survival. Furthermore, the expression of hPAF1C was found to be positively correlated with c-MYC expression in patient tumor samples and in cancer cell lines. Mechanistic studies indicated that hPAF1C could promote lung cancer cell proliferation through regulating c-MYC transcription. These results demonstrated the prognostic value of hPAF1C in early-stage NSCLC and the role of hPAF1C in the transcriptional regulation of c-MYC oncogene during NSCLC tumorigenesis.
Department of Thoracic Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
Xiuyi
X
Zhi
ER2 GRC UPMC04 Theranoscan, Pierre et Marie Curie University, Tenon Hospital, 4 Rue de La Chine, 75020, Paris, France; Respiratory Diseases and Thoracic Oncology Department, Ambroise Pare Hospital - APHP, Versailles Saint Quentin en Yvelines University, 9 Avenue Charles de Gaulle, 92100, Boulogne-Billancourt, France.
Etienne
E
Giroux-Leprieur
ER2 GRC UPMC04 Theranoscan, Pierre et Marie Curie University, Tenon Hospital, 4 Rue de La Chine, 75020, Paris, France.
Marie
M
Wislez
Department of Thoracic Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
Mu
M
Hu
Department of Thoracic Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
Yi
Y
Zhang
Department of Pathology, Chinese PLA General Hospital, Fu-xing Road #28, Beijing, 100853, China.
Huaiyin
H
Shi
Department of Cardiothoracic Surgery, Chinese People's Armed Police Force, Zhejiang Corps Hospital, Jiaxing, Zhejiang Province, China. Electronic address: kaiqidu_zhejiang@163.com.
Kaiqi
K
Du
Department of Human Anatomy, Hebei Medical University, Hebei Province, China. Electronic address: leiwang_hebei@163.com.
Lei
L
Wang
D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D016259:Genes, myc; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors; D015533:Transcriptional Activation; D014410:Tumor Stem Cell Assay
D009687:Nuclear Proteins; C501972:PAF1 protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors
C-MYC; Non-small cell lung cancer; Prognosis; Tumorigenesis; hPAF1C