Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces.
Increasing drying time adversely affects attachment of tissue proteins and prion-associated amyloid to surgical stainless steel, and reduces the efficacy of commercial cleaning chemistries. This study tested the efficacy of commercial humidity retention bags to reduce biofouling on surgical stainless steel and to improve subsequent cleaning. Surgical stainless steel surfaces were contaminated with ME7-infected brain homogenates and left to dry for 15 to 1,440 min either in air, in dry polythene bags or within humidity retention bags. Residual contamination pre/post cleaning was analysed using Thioflavin T/SYPRO Ruby dual staining and microscope analysis. An increase in biofouling was observed with increased drying time in air or in sealed dry bags. Humidity retention bags kept both protein and prion-associated amyloid minimal across the drying times both pre- and post-cleaning. Therefore, humidity bags demonstrate a cheap, easy to implement solution to improve surgical instrument reprocessing and to potentially reduce associated hospital acquired infections.
a Environmental Healthcare Unit, Centre for Biological Sciences , University of Southampton , Southampton , UK.
T J
TJ
Secker
H E
HE
Pinchin
R C
RC
Hervé
C W
CW
Keevil
D000327:Adsorption; D000682:Amyloid / Q000032:analysis*; D057219:Biofouling; D003428:Cross Infection / Q000517:prevention & control; D003666:Decontamination / Q000379:methods*; D003890:Desiccation; D004866:Equipment Contamination / Q000517:prevention & control*; D006801:Humans; D006813:Humidity; D011328:Prions / Q000032:analysis*; D013194:Staining and Labeling; D013193:Stainless Steel / Q000737:chemistry*; D013525:Surgical Instruments / Q000592:standards*
D000682:Amyloid; D011328:Prions; D013193:Stainless Steel
contamination; decontamination; hospital infections; humidity; prions; proteins