Differentiating characteristic microstructural features of cancerous tissues using Mueller matrix microscope.
Polarized light imaging can provide rich microstructural information of samples, and has been applied to the detections of various abnormal tissues. In this paper, we report a polarized light microscope based on Mueller matrix imaging by adding the polarization state generator and analyzer (PSG and PSA) to a commercial transmission optical microscope. The maximum errors for the absolute values of Mueller matrix elements are reduced to 0.01 after calibration. This Mueller matrix microscope has been used to examine human cervical and liver cancerous tissues with fibrosis. Images of the transformed Mueller matrix parameters provide quantitative assessment on the characteristic features of the pathological tissues. Contrast mechanism of the experimental results are backed up by Monte Carlo simulations based on the sphere-cylinder birefringence model, which reveal the relationship between the pathological features in the cancerous tissues at the cellular level and the polarization parameters. Both the experimental and simulated data indicate that the microscopic transformed Mueller matrix parameters can distinguish the breaking down of birefringent normal tissues for cervical cancer, or the formation of birefringent surrounding structures accompanying the inflammatory reaction for liver cancer. With its simple structure, fast measurement and high precision, polarized light microscope based on Mueller matrix shows a good diagnosis application prospect.
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; Department of Physics, Tsinghua University, Beijing 100084, China.
Ye
Y
Wang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Honghui
H
He
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; Department of Physics, Tsinghua University, Beijing 100084, China.
Jintao
J
Chang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Nan
N
Zeng
Shenzhen Sixth People's Hospital (Nanshan Hospital), Shenzhen 518052, China.
Shaoxiong
S
Liu
Guangzhou Liss Optical Instrument Factory, Guangzhou 510095, China.
Migao
M
Li
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; Department of Physics, Tsinghua University, Beijing 100084, China. Electronic address: mahui@tsinghua.edu.cn.
Hui
H
Ma
D001718:Birefringence; D002138:Calibration; D003198:Computer Simulation; D005260:Female; D005355:Fibrosis; D006801:Humans; D007249:Inflammation; D008099:Liver / Q000648:ultrastructure*; D008113:Liver Neoplasms / Q000473:pathology / Q000648:ultrastructure*; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000648:ultrastructure
Cancerous tissues; Fibrosis; Microscope; Mueller matrix; Polarization