Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.
Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Anizah
A
Rahumatullah
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Azimah
A
Ahmad
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Rahmah
R
Noordin
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: theamsoon@usm.my.
Theam Soon
TS
Lim
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000235:genetics* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D017178:Brugia malayi / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000276:immunology* / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D005854:Germ Cells / Q000378:metabolism; D006801:Humans; D007114:Immunization; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D022801:Complementarity Determining Regions; D019151:Peptide Library; D057127:Single-Chain Antibodies
Immune antibody library; Lymphatic filariasis; Phage display; V-gene usage