Fungal community dynamics and driving factors during agricultural waste composting.
This study was conducted to identify the driving factors behind fungal community dynamics during agricultural waste composting. Fungal community abundance and structure were determined by quantitative PCR and denaturing gradient gel electrophoresis analysis combined with DNA sequencing. The effects of physico-chemical parameters on fungal community abundance and structure were evaluated by least significant difference tests and redundancy analysis. The results showed that Cladosporium bruhnei, Hanseniaspora uvarum, Scytalidium thermophilum, Tilletiopsis penniseti, and Coprinopsis altramentaria were prominent during the composting process. The greatest variation in the distribution of fungal community structure was statistically explained by pile temperature and total organic carbon (TOC) (P < 0.05). A significant amount of the variation (74.6 %) was explained by these two parameters alone. Fungal community abundance was found to be significantly related to pH, while pH was significantly influenced by pile temperature and nitrate levels (P < 0.05), and these parameters were found to be the most likely to influence or be influenced by the fungal community during composting.
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. yuman20030703@aliyun.com.
Man
http://orcid.org/0000-0003-2777-3620
M
Yu
College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China. jiachao.zhang@163.com.
Jiachao
J
Zhang
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Yuxin
Y
Xu
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Hua
H
Xiao
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Wenhao
W
An
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Hui
H
Xi
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Zhiyong
Z
Xue
College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China. ahuanghongli@163.com.
Hongli
H
Huang
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Xiaoyang
X
Chen
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. shenalin_123@126.com.
Alin
A
Shen
D000383:Agriculture / Q000379:methods*; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000032:analysis; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013696:Temperature; D014866:Waste Products / Q000032:analysis*
D009566:Nitrates; D012987:Soil; D014866:Waste Products
Composting; Driving factor; Fungal community; Population dynamic; Redundancy analysis