Interaction between lanthanide ions and Saccharomyces cerevisiae cells.
Lanthanides are a group of non-essential elements with important imaging and therapeutic applications. Although trivalent lanthanide ions (Ln³⁺) are used as potent blockers of Ca²⁺ channels, the systematic studies correlating Ln³⁺ accumulation and toxicity to Ca²⁺ channel blocking activity are scarce. In this study, we made use of the eukaryotic model Saccharomyces cerevisiae to investigate the correlation between Ln³⁺ accumulation, their toxicity and their capacity to block the exogenous stress-induced Ca²⁺ influx into the cytosol. It was found that the Ln³⁺ blocked the Ca²⁺ entry into the yeast cells only when present at concentration high enough to allow rapid binding to cell surface. At lower concentrations, Ln³⁺ were taken up by the cell, but Ca²⁺ blockage was no longer achieved. At 1 mM concentration, all ions from the Ln³⁺ series could block Ca²⁺ entry into cytosol with the exception of La³⁺, and to a lesser extent, Pr³⁺ and Nd³⁺. The plasma membrane Ca²⁺-channel Cch1/Mid1 contributed to La³⁺ and Gd³⁺ entry into the cells, with a significant preference for La³⁺. The results open the possibility to obtain cells loaded with controlled amounts and ratios of Ln³⁺.
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Cristian D
CD
Ene
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Lavinia L
LL
Ruta
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Ioana
I
Nicolau
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Claudia V
CV
Popa
Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania.
Virgil
V
Iordache
Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania.
Aurora D
AD
Neagoe
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania. ileana.farcasanu@chimie.unibuc.ro.
Ileana C
IC
Farcasanu
D002118:Calcium / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D007477:Ions / Q000494:pharmacology; D028581:Lanthanoid Series Elements / Q000737:chemistry* / Q000633:toxicity; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects*
D007477:Ions; D028581:Lanthanoid Series Elements; D002118:Calcium
Aequorin; Alkaline stress; Calcium signaling; Lanthanide; Saccharomyces cerevisiae