A comparative study on biologically and chemically synthesized silver nanoparticles induced Heat Shock Proteins on fresh water fish Oreochromis niloticus.
The wide applicability of silver nanoparticles in medicine and pharmaceutical industries leads to its over exploitation and thus contaminating our environment. Majority of these nanoscale dimension particles finally accumulates in fresh water and marine ecosystem. As the nanoparticles behave entirely different from its corresponding bulk material, a better understanding of their environmental impacts in aquatic ecosystems is inevitable. The study was focused on a comparative stress physiology analysis of chemically synthesized silver nanoparticles and biogenic silver nanoparticles. Half maximal inhibitory concentration of biologically synthesized and chemically synthesized nanoparticles was found out (30μg/mL and 20μg/mL respectively). The Heat Shock Protein (HSP70) secretion was analysed in the fresh water fish Oreochromis niloticus after exposing to different concentrations of biologically and chemically synthesized silver nanoparticles along with the silver in its ionic form. The intense immune-histochemical staining of fish tissues (muscle, kidney and liver) analyzed proportionately reflected the stress created. The colour intensity was directly proportional to the stress created or the stress protein released. High level of HSP70 expression was observed in all of the fish tissues exposed to silver ions and chemically synthesized silver nanoparticles, when compared to that of biologically synthesized. The results revealed the significance of comparatively safe and less toxic biogenic nanoparticles compared to the chemically synthesized.
CAS in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India. Electronic address: girilal@gmail.com.
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Girilal
Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
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Krishnakumar
Department of Biotechnology, Bharathiyar University, Coimbatore, Tamil Nadu, India.
Paramasivan
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Poornima
Laboratory for Food Chemistry & Microbiology, Department of Food and Nutrition Science, Pavillon Paul Comtois, Universite Laval, Quebec G1K7P4, Canada.
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Mohammed Fayaz
ALKA-RESEARCH FOUNDATION, 3/137/A1 Anna Nagar, Maruthamalai Adivaaram, Coimbatore 641 046, Tamil Nadu, India.
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Kalaichelvan
D000818:Animals; D060326:Chemistry Techniques, Synthetic; D023681:Cichlids / Q000378:metabolism*; D054750:Ecotoxicology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000633:toxicity*; D009928:Organ Specificity; D012834:Silver / Q000737:chemistry* / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000633:toxicity*
D018840:HSP70 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D012834:Silver
Biogenic; Heat Shock Proteins; Oreochromis niloticus; Silver nanoparticles; Toxicity