CT26 murine colon carcinoma expressing the red fluorescent protein KillerRed as a highly immunogenic tumor model.
The development of tumor therapies based on the activation of antitumor immunity requires tumor models that are highly immunogenic. The immunologic response to fluorescent proteins, green fluorescent protein (GFP), or enhanced GFP (EGFP) was demonstrated in different cancer models. However, for live animal imaging, red and far-red fluorescent proteins are preferable, but their immunogenicity has not been studied. We assessed the immunogenicity of the red fluorescent protein, KillerRed (KR), in CT26 murine colon carcinoma. We showed a slower growth and a lower tumor incidence of KR-expressing tumors in comparison with nonexpressing ones. We found that KR-expressing lung metastases and rechallenged tumors were not formed in mice that had been surgically cured of KR-expressing primary tumors. The effect of low-dose cyclophosphamide (CY) treatment was also tested, as this is known to activate antitumor immune responses. The low-dose CY therapy of CT26-KR tumors resulted in inhibition of tumor growth and improved mouse survival. In summary, we have established a highly immunogenic tumor model that could be valuable for investigations of the mechanisms of antitumor immunity and the development of new therapeutic approaches.
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, RussiabLobachevsky State University of Nizhny Novgorod, Gagarina Avenue, 23, Nizhny Novgorod 603950, Russia.
Diana V
DV
Yuzhakova
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, Russia.
Marina V
MV
Shirmanova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russia.
Ekaterina O
EO
Serebrovskaya
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, RussiacShemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russi.
Konstantin A
KA
Lukyanov
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, Russia.
Irina N
IN
Druzhkova
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, Russia.
Boris E
BE
Shakhov
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, RussiacShemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russi.
Sergey A
SA
Lukyanov
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Minin and Pozharsky Square, 10/1, Nizhny Novgorod 603005, Russia.
Elena V
EV
Zagaynova
D000818:Animals; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D018448:Models, Immunological; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology*
C508771:killer red protein, Anthomedusae; D049452:Green Fluorescent Proteins