The role of high mobility group box 1 (HMGB-1) in the diabetic retinopathy inflammation and apoptosis.
Diabetic Retinopathy (DR) is one of the most common complications of the late phase diabetes, and also a common cause of blindness. High mobility group box 1 (HMGB-1) is considered to be an inflammatory mediator in the late phase that promotes inflammation and neovascularization in diabetes. Therefore, this paper discussed the role of HMGB-1 in diabetic retinopathy inflammation and neovascularization. 96 adult SD rats were randomly divided into control and diabetes group. The diabetic rat model was established by intraperitoneal injection of streptomycin (0.1 mol/L). Western blot was applied to determine HMGB-1 and its receptor RAGE and TLR2 protein expression in the serum. TUNEL was used to detect retinal apoptosis. Immunofluorescence was performed to test HMGB1 protein expression in retina. HBGM-1 and RAGE expression in diabetic rat retina was significantly higher than the control (P < 0.05), while TLR2 expression was lower (P < 0.05). TUNEL detection showed that diabetic rat retinal cells presented obviously higher apoptosis rate (P < 0.05). Immunofluorescence test revealed that HMGB1 largely expressed in the diabetic rat retinal cells (P < 0.05). HMGB1 may involve in the pathogenesis of diabetic retinopathy by binding with RAGE receptor to accelerate rat retinal cells apoptosis.
Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute Nanchang 330006, Jiangxi Province, China ; Department of Endocrinology and Metabolism, The Third Hospital of Nanchang, Nanchang Key Laboratory of Diabetes Nanchang 330009, Jiangxi Province, China.
Yao
Y
Yu
Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute Nanchang 330006, Jiangxi Province, China ; Affiliated Eye Hospital of Nanchang University, Jiangxi Province Vision Science and Ophthalmology Institute Nanchang 330006, Jiangxi Province, China.
Lu
L
Yang
Department of Nephrology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute Nanchang 330006, Jiangxi Province, China.
Jinlei
J
Lv
Affiliated Eye Hospital of Nanchang University, Jiangxi Province Vision Science and Ophthalmology Institute Nanchang 330006, Jiangxi Province, China.
Xu
X
Huang
Affiliated Eye Hospital of Nanchang University, Jiangxi Province Vision Science and Ophthalmology Institute Nanchang 330006, Jiangxi Province, China.
Jinglin
J
Yi
Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute Nanchang 330006, Jiangxi Province, China.
Chonggang
C
Pei
Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Jiangxi Province Clinical Ophthalmology Institute Nanchang 330006, Jiangxi Province, China.
Yi
Y
Shao
D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003930:Diabetic Retinopathy / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017207:Rats, Sprague-Dawley; D000067759:Receptor for Advanced Glycation End Products / Q000097:blood; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D012173:Retinitis / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000097:blood
C000593874:Ager protein, rat; D051017:Apoptosis Regulatory Proteins; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D018836:Inflammation Mediators; D000067759:Receptor for Advanced Glycation End Products; C495343:Tlr2 protein, rat; D051195:Toll-Like Receptor 2
Diabetes; HMGB-1; apoptosis; retinal inflammation