Silencing of tripartite motif (TRIM) 29 inhibits proliferation and invasion and increases chemosensitivity to cisplatin in human lung squamous cancer NCI-H520 cells.
BACKGROUND
TRIM29 belongs to the tripartite motif (TRIM) protein family. It has been reported to be a tumor suppressor or have oncogenic function in many cancer types. The aim of this study was to investigate whether downregulation of TRIM29 by small interfering ribonucleic acid (siRNA) could inhibit cell proliferation and invasion and increase chemosensitivity to cisplatin in human lung squamous cancer NCI-H520 cells in vitro.


METHODS
We transformed TRIM29 siRNA into NCI-H520 cells. Real time reverse transcriptase polymerase chain reaction and Western blotting assay were employed to determine TRIM29 messenger (m)RNA and protein expressions. MTT assay was used to determine the cell proliferation. Transwell invasion assay was used to determine the cell invasion. An Annexin V-propidium iodide (AnnV/PI) staining apoptosis test was used for detecting apoptosis.


RESULTS
TRIM29 siRNA could specifically and efficiently suppress TRIM29 expression at both mRNA and protein levels. Silencing of the TRIM29 by siRNA in NCI-H520 cells inhibited cell proliferation and invasion in vitro. TRIM29 knockdown resulted in chemosensitivity enhancement in NCI-H520 cells.


CONCLUSIONS
Downregulation of TRIM29 can lead to potent antitumor activity and chemosensitizing effect in human lung squamous cancer NCI-H520 cells.
Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Capital Medical University Beijing, China.
Chunxiao
C
Liu
Department of Medical Research Center, Beijing Chao-Yang Hospital, Capital Medical University Beijing, China.
Xiaoxi
X
Huang
Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Capital Medical University Beijing, China.
Shengcai
S
Hou
Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Capital Medical University Beijing, China.
Bin
B
Hu
Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Capital Medical University Beijing, China.
Hui
H
Li
Chemosensitivity; TRIM29; lung cancer; siRNA