Adequacy of Petrifilm™ Aerobic Count plates supplemented with de Man, Rogosa & Sharpe broth and chlorophenol red for enumeration of lactic acid bacteria in salami.
The present study aimed to assess the performance of alternative protocols to enumerate lactic acid bacteria (LAB) in salami. Fourteen cultures and two mixed starter cultures were plated using six protocols: 1) Petrifilm™ Aerobic Count (AC) with MRS broth and chlorophenol red (CR), incubated under aerobiosis or 2) under anaerobiosis, 3) MRS agar with CR, 4) MRS agar with bromocresol purple, 5) MRS agar at pH5.7, and 6) All Purpose Tween agar. Samples of salami were obtained and the LAB microbiota was enumerated by plating according protocols 1, 2, 3 and 5. Regression analysis showed a significant correlation between the tested protocols, based on culture counts (p<0.05). Similar results were observed for salami, and no significant differences of mean LAB counts between selected protocols (ANOVA, p>0.05). Colonies were confirmed as LAB, indicating proper selectivity of the protocols. The results showed the adequacy of Petrifilm™ AC supplemented with CR for the enumeration of LAB in salami.
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570 900 Viçosa, MG, Brazil.
Natália Parma Augusto
NP
de Castilho
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570 900 Viçosa, MG, Brazil.
Vivian Tiemi
VT
Okamura
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570 900 Viçosa, MG, Brazil.
Anderson Carlos
AC
Camargo
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570 900 Viçosa, MG, Brazil; Universidade Federal de Juiz de Fora, Departamento de Ciências Básicas da Saúde, Campus Governador Valadares, 35020 220 Governador Valadares, MG, Brazil.
Fábio Alessandro
FA
Pieri
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570 900 Viçosa, MG, Brazil. Electronic address: nero@ufv.br.
Luís Augusto
LA
Nero
D001431:Bacteriological Techniques / Q000295:instrumentation*; D004396:Coloring Agents / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D017045:Lactococcus / Q000302:isolation & purification*; D008461:Meat Products / Q000382:microbiology*; D010637:Phenolsulfonphthalein / Q000031:analogs & derivatives*
D004396:Coloring Agents; D003470:Culture Media; C007016:chlorophenol red; D010637:Phenolsulfonphthalein
Enumeration; Lactic acid bacteria; Petrifilm™ AC; Salami