A dual-FRET-based fluorescence probe for the sequential detection of MMP-2 and caspase-3.
A novel dual-FRET-based fluorescence probe was designed and synthesized for the sequential detection of MMP-2 and caspase-3. This probe can successfully monitor Dox- and UV-induced cell apoptosis signals in real time and in situ, which demonstrated its great potential for apoptosis-related drug screening and dynamic apoptosis imaging.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. xz-zhang@whu.edu.cn.
Shi-Ying
SY
Li
Li-Han
LH
Liu
Hong
H
Cheng
Bin
B
Li
Wen-Xiu
WX
Qiu
Xian-Zheng
XZ
Zhang
D000818:Animals; D019556:COS Cells; D053148:Caspase 3 / Q000032:analysis*; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*
D005456:Fluorescent Dyes; D053148:Caspase 3; D020778:Matrix Metalloproteinase 2