Polyphenolic profile and antioxidant effects of various parts of Artemisia annua L.
An annual Korean weed, Artemisia annua L., has been used as a folk medicine for the treatment of a number of diseases. Remarkably, among the 32 polyphenols characterized in various parts of plant tissue, including flowers, leafs, stems and roots, 10 compounds were detected for the first time using liquid chromatography-tandem mass spectrometry (LC/MS/MS). The quantification method was validated using structurally related external standards with determination coefficients (R(2) ) ≥0.9995. The limits of detection and quantitation were 0.068-3.932 and 0.226-13.108 mg/L, respectively. The recoveries estimated at 50 and 100 mg/L ranged between 60.6-92.2 and 61.3-111%, respectively, with relative standard deviations <12%. The roots contained the largest concentration of identified components, while the flowers contained the least. The antioxidant capacity evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activities and reducing power was highest in the roots and lowest in the flowers. The findings are well correlated and suggest that the antioxidant capacities principally depend upon the polyphenol concentrations in each part of the plant.
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Yi
Y
Song
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Kebede Taye
KT
Desta
Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Gon-Sup
GS
Kim
Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Soo Jung
SJ
Lee
Department of Internal Medicine, Institute of Health Sciences and Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju, 660-702, Republic of Korea.
Won Sup
WS
Lee
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Yun-Hi
YH
Kim
Division of High Technology Materials Research, Busan Center, Korea Basic Science Institute (KBSI), Busan, 618-230, Republic of Korea.
Jong Sung
JS
Jin
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, Republic of Korea.|Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt.
A M
AM
Abd El-Aty
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, Republic of Korea.
Ho-Chul
HC
Shin
Biotechnology Research Institute, College of Agriculture and Life Science, Chonnam National University, Yongbong-ro 77, Buk-gu, 500-757, Gwangju, Republic of Korea.
Jae-Han
JH
Shim
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Sung Chul
SC
Shin
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D031172:Artemisia annua / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D057230:Limit of Detection; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
Artemisia annua L; antioxidant activity; liquid chromatography-tandem mass spectrometry; medicinal herb; polyphenols