Comparative transcriptomic analysis between an artificially induced SIRS in healthy individuals and spontaneous sepsis.
Sepsis is defined as a syndrome combining a systemic inflammatory response with a documented infection. It may progress to more serious cases such as septic shock following the failure of one or more organs and the emergence of hemodynamic defects. Assuming that the emergence of serious septic syndromes may be partially explained by the early loss of regulation of the inflammatory response, we decided to compare, in a transcriptomic perspective, the biological mechanisms expressed during an induced systemic inflammatory response with those expressed during severe septic syndromes. By using open-access transcriptomic databases, we first studied the kinetics of an induced inflammatory response. The use of functional analysis helped us identify discriminating biological mechanisms, such as the mTOR signaling pathway, between the pathological cases of sepsis and non-pathological (i.e., the artificially induced SIRS) cases.
Novadiscovery, 60, avenue Rockefeller, 69008 Lyon, France; Université Claude Bernard Lyon 1, CNRS, UMR 5558, laboratoire de biométrie et biologie évolutive, équipe « Évaluation et modélisation des effets thérapeutiques », 7, rue Guillaume-Paradin, 69007 Lyon, France. Electronic address: claudio.ms@universite-lyon.fr.
Claudio
C
Monteiro Sousa
Novadiscovery, 60, avenue Rockefeller, 69008 Lyon, France.
Jean-Pierre
JP
Boissel
Université Claude Bernard Lyon 1, CNRS, UMR 5558, laboratoire de biométrie et biologie évolutive, équipe « Évaluation et modélisation des effets thérapeutiques », 7, rue Guillaume-Paradin, 69007 Lyon, France.
François
F
Gueyffier
Novadiscovery, 60, avenue Rockefeller, 69008 Lyon, France.
Gustavo
G
Olivera-Botello
D056704:Adaptive Immunity / Q000235:genetics; D000328:Adult; D017209:Apoptosis / Q000235:genetics; D066264:Datasets as Topic; D019446:Endotoxemia / Q000139:chemically induced / Q000150:complications* / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D046228:Microarray Analysis; D005810:Multigene Family; D011183:Postoperative Complications / Q000235:genetics; D025341:Principal Component Analysis; D011446:Prospective Studies; D018805:Sepsis / Q000150:complications* / Q000235:genetics; D015398:Signal Transduction; D018746:Systemic Inflammatory Response Syndrome / Q000209:etiology / Q000235:genetics*; D058570:TOR Serine-Threonine Kinases / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D059467:Transcriptome
D014157:Transcription Factors; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
Analyse fonctionnelle; Functional analysis; Inflammation; SIRS; SRIS; Sepsis; Transcriptome