Multiwall Carbon Nanotube-Induced DNA Damage and Cytotoxicity in Male Human Peripheral Blood Lymphocytes.
Carbon nanotubes (CNTs) have been introduced recently as a novel carrier system for both small and large therapeutic molecules. Biotin-functionalized single-wall CNTs have been conjugated with the anticancer agent taxoid using a cleavable linker, and multiwall carbon nanotubes (MWCNTs) conjugated with iron nanoparticles have been efficiently loaded with doxorubicin. The MWCNTs are effective transporters for biological macromolecules and drugs to target cells and tissues, thereby attracting the attention of the biomedical industry. Administrating MWCNTs for medical application invariably involves intravenous administration and ultimate contact with human peripheral blood lymphocytes (HPBLs), yet toxicological studies on the effect of MWCNTs on HPBLs are lacking. Accordingly, this study evaluated the cytotoxic and genotoxic effects of MWCNTs on healthy male HPBLs. Healthy male HPBLs were treated with MWCNTs at 3 different concentrations (12.5, 25, and 50 μg/mL) for 48 hours. Under these conditions, the MWCNTs induced significant cell growth retardation, DNA damage, and cytotoxicity. The MWCNT-treated HPBLs also exhibited an increased intracellular reactive oxygen species level during the experimental period, which leads to cell damage and death, proliferation inhibition, DNA damage, and an inflammatory response.
Bioconvergence Laboratory, Korea Conformity Laboratories, Incheon, Korea.
Jin Sik
JS
Kim
Bioconvergence Laboratory, Korea Conformity Laboratories, Incheon, Korea.
Kyung Seuk
KS
Song
Institute of Nanoproduct Safety Research, Hoseo University, Asan, Korea u1670916@chollian.net.
Il Je
IJ
Yu
D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D037742:Nanotubes, Carbon / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism
D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species
DNA damage; cell growth; cytotoxicity; multiwall carbon nanotubes (MWCNTs); peripheral blood lymphocytes; reactive oxygen species (ROS)