Resveratrol potentiates the effect of dexamethasone in rat model of acute lung inflammation.
Cigarette smoking is considered to be the main etiological factor in Chronic Obstructive Pulmonary Disease (COPD). In this study, we explored the potential of resveratrol, to reinstate the effectiveness of dexamethasone when administered as an adjunct in acute lung inflammation induced by cigarette smoke (CS) and lipopolysaccharide (LPS). CS and LPS instillation produced acute inflammatory response exhibited by increased leukocyte count, particularly neutrophils, total protein, MMP-9 activity, cytokines like TNF-α, IL-8 in bronchoalveolar lavage fluid (BALF) as well as elevated myeloperoxidase activity, and lipid peroxidation in lung. These alterations were not abated by dexamethasone (2.5mg/kg & 10mg/kg) and resveratrol (50mg/kg) alone. Combination of resveratrol (50mg/kg) and dexamethasone (2.5mg/kg) significantly reduced all inflammatory parameters. The protective effect of the combination was abolished when co-administered with sirtinol, a SIRT1 inhibitor. The results indicate that the combination therapy may serve as a potential approach for treating lung inflammatory conditions like COPD.
Department of Pharmacology, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400068, Maharashtra, India. Electronic address: bhakti.sadarani@gmail.com.
Bhakti N
BN
Sadarani
Department of Pharmacology, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400068, Maharashtra, India. Electronic address: anuradha.majumdar@gmail.com.
Anuradha S
AS
Majumdar
D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003907:Dexamethasone / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D011014:Pneumonia / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D012906:Smoke; D013267:Stilbenes / Q000008:administration & dosage / Q000627:therapeutic use*; D062789:Tobacco Products / Q000633:toxicity*
D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D012906:Smoke; D013267:Stilbenes; C440499:lipopolysaccharide, Escherichia coli O111 B4; D003907:Dexamethasone; D000077185:Resveratrol
Cigarette smoke; Dexamethasone; Lipopolysaccharide; Matrix metalloproteinase-9; Resveratrol