Antihypertensive Effect of a Combination of Uracil and Glycerol Derived from Lactobacillus plantarum Strain TWK10-Fermented Soy Milk.
We previously demonstrated that angiotensin-converting enzyme (ACE) could be inhibited by soy milk that had been fermented with the Lactobacillus plantarum strain TWK10, suggesting great potential for the development of antihypertensive products. In this work, the bioactive ACE inhibitors in TWK10-fermented soy milk water extracts were isolated, and a combination of uracil and glycerol (CUG) was identified as one of the ACE inhibitors. We then examined the physiological effects of CUG treatment in short-term and long-term studies using spontaneously hypertensive rats (SHRs) as an experimental model. The results revealed that the fermented soy milk extracts and CUG decreased blood pressure by 11.97 ± 3.71 to 19.54 ± 9.54 mmHg, 8 h after oral administration, and exhibited antihypertensive effects in SHRs in a long-term study. In addition, CUG was shown to decrease blood pressure by suppressing either the renin activity or the ACE activity and, thus, decreasing the downstream vasoconstricting peptide angiotensin II and the hormone aldosterone. CUG also promoted nitric oxide production, resulting in vasodilation and further improvement to hypertension. This important finding suggests that TWK10-fermented soy milk and its functional ingredients, uracil and glycerol, exhibit antihypertensive effects via multiple pathways and provide a healthier and more natural antihypertensive functional food.
Department of Food Science, Fu Jen Catholic University , New Taipei City, Taiwan.
Yi-Yen
YY
Liu
Department of Food Science, Fu Jen Catholic University , New Taipei City, Taiwan.
Shih-Yu
SY
Zeng
Graduate Institute of Natural Products, Chang Gung University , Taoyuan City, Taiwan.
Yann-Lii
YL
Leu
Department of Food Science, Fu Jen Catholic University , New Taipei City, Taiwan.
Tsung-Yu
TY
Tsai
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D004359:Drug Therapy, Combination; D005285:Fermentation; D005990:Glycerol / Q000494:pharmacology*; D006973:Hypertension / Q000188:drug therapy; D048191:Lactiplantibacillus plantarum / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D041942:Soy Milk / Q000737:chemistry / Q000494:pharmacology*; D014498:Uracil / Q000494:pharmacology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D000975:Antioxidants; D009569:Nitric Oxide; D014498:Uracil; D007703:Peptidyl-Dipeptidase A; D005990:Glycerol
TWK10-fermented soy milk; angiotensin-converting enzyme; combination of uracil and glycerol; hypertension; spontaneous hypertensive rats