A ferrocene-switched electrochemiluminescence "off-on" strategy for the sensitive detection of cardiac troponin I based on target transduction and a DNA walking machine.
A ferrocene-switched electrochemiluminescence "off-on" sensor is proposed on the basis of target transduction and a DNA walking machine for the sensitive detection of cardiac troponin I (cTnI). The proposed method offers high selectivity with a low detection limit of 0.016 pg mL(-1), and provides a universal platform for detecting a wide range of targets, including other proteins and DNAs, by changing the molecular recognition elements to the corresponding antibodies, aptamers or DNA probes.
Key Laboratory on Luminescence and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yuanruo@swu.edu.cn.
Ziqi
Z
Xu
Yongwang
Y
Dong
Jiyang
J
Li
Ruo
R
Yuan
D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D000075163:Metallocenes; D019210:Troponin I / Q000032:analysis*
D052157:Aptamers, Nucleotide; D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; D019210:Troponin I; D004247:DNA; C004998:ferrocene