The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging-a retrospective observational study.
OBJECTIVE
Various time-lapse studies have postulated embryo selection criteria based on early morphokinetic markers. However, late paternal effects are mostly not visible before embryonic genome activation. The primary objective of this retrospective study was to investigate whether those early morphokinetic algorithms investigated by time-lapse imaging are reliable enough to allow for the accurate selection of those embryos that develop into blastocysts, while of course taking into account the correlation with the type of injected spermatozoa.


METHODS
During a period of 18 months, a total of 461 MII oocytes from 43 couples with severe male factor infertility and previous "external" IVF failures after cleavage-stage embryo transfer (ET) were fertilized by intracytoplasmic morphologically selected sperm injection (IMSI). Thereof, 373 embryos were monitored in a time-lapse incubator until ET on day 5. Blastocyst outcome in combination with three previously postulated MKc (cc2: t3-t2, 5-12 h; t3, 35-40 h; t5, 48-56 h) and the morphology of the selected sperm were analyzed.


RESULTS
A significant increase in the rate of blastocysts (54.0 vs. 36.3 %; P < 0.01) and top blastocysts (25.3 vs. 10.8 %; P < 0.001) was observed in the group of those meeting all three morphokinetic criteria (MKc3). However, MKc3 were only met in 23.3 % of all embryos. Moreover, TBR was influenced by the type of injected spermatozoa. In both groups, TBR decreased dramatically (MKc3, 35.0 vs. 17.0 %; MKc < 3, 14.2 vs. 8.4 %) when class II/III sperm instead of class I were injected.


CONCLUSIONS
Early morphokinetic parameters might give some predictive information but fail to serve as a feasible selective tool for the prediction of blastocyst development given the influence of the type of spermatozoa injected.
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria. a.neyer@ivf.at.
Anton
A
Neyer
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria.
Martin
M
Zintz
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria.
Astrid
A
Stecher
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria.
Magnus
M
Bach
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria.
Barbara
B
Wirleitner
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria.
Nicolas H
NH
Zech
IVF Centers Prof. Zech-Bregenz, Römerstrasse 2, 6900, Bregenz, Austria.|Centre Hospitalier Interrégional Edith Cavell (CHIREC), Braine-l'Alleud, Brussels, Belgium.
Pierre
P
Vanderzwalmen
D000328:Adult; D000465:Algorithms; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007248:Infertility, Male / Q000209:etiology; D008297:Male; D008875:Middle Aged; D009865:Oocytes; D010331:Paternal Age; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013094:Spermatozoa / Q000502:physiology*; D059008:Time-Lapse Imaging / Q000379:methods*
Blastocyst; ICSI; Morphokinetics; Paternal effect; Sperm; Time-lapse cinematography