Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil.
The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a nested-PCR reaction (n-PCR) and sequencing. In the immunofluorescence, 2/24 (8.33%) samples of raw water were positive for Giardia spp.. In n-PCR and sequencing, 2/24 (8.33%) samples of raw water were positive for Giardia spp., and 2/24 (8.33%) samples were positive for Cryptosporidium spp.. The sequencing showed Cryptosporidium parvum and Giardia duodenalis DNA. In raw water, there was moderate correlation among turbidity, color and Cryptosporidium spp. and between turbidity and Giardia spp.. The presence of these protozoans in the water indicates the need for monitoring for water-treatment companies.
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
Jonatas Campos
JC
Almeida
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
Felippe Danyel Cardoso
FD
Martins
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
José Maurício
JM
Ferreira Neto
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
Maíra Moreira Dos
MM
Santos
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
João Luis
JL
Garcia
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
Italmar Teodorico
IT
Navarro
Departamento de Construção Civil, Universidade Estadual de Londrina, Londrina, PR, BR.
Emília Kiyomi
EK
Kuroda
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, BR.
Roberta Lemos
RL
Freire
D001938:Brazil; D003458:Cryptosporidium / Q000302:isolation & purification*; D005872:Giardia / Q000302:isolation & purification*; D014867:Water / Q000469:parasitology*; D018508:Water Purification
D014867:Water