[Role of calmodulin-dependent protein kinase II in bupivacaine hydrochloride-induced injury of SH-SY5Y cells].
OBJECTIVE
To investigate the effect of KN93, a calmodulin-dependent protein kinase II (CaMK II) inhibitor, on SH-SY5Y cell injury induced by bupivacaine hydrochloride.


METHODS
SH-SY5Y cells exposed for 24 h to 1 mmol/L KN93, 1 mmol/L bupivacaine hydrochloride, or both were examined for morphological changes and Cav3.1 protein expressions using Western blotting. The vitality and apoptosis rate of the cells at different time points during the exposures were assessed with MTT assay and flow cytometry, respectively.


RESULTS
Bupivacaine hydrochloride exposure caused obvious cell morphologial changes, reduced cell viability, increased cell apoptosis, and enhanced Cav3.1 protein expression. All these changes were partly reversed by treatment of the cells with 1 mmol/L KN93.


CONCLUSIONS
CaMKII may play a role in bupivacaine hydrochloride-induced SH-SY5Y cells injury, which is related with upregulated Cav3.1 protein expression.
Department of Anesthesiology, Foshan First People's Hospital, Foshan 528000, China. E-mail: xjwen166@163.com.
Xianjie
X
Wen
Jiying
J
Zhong
Tao
T
Zhang
Xiaohong
X
Lai
Hongzhen
H
Liu
Hanbing
H
Wang
Chengxiang
C
Yang
D017209:Apoptosis; D002045:Bupivacaine / Q000009:adverse effects*; D020747:Calcium Channels, T-Type / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D015854:Up-Regulation
C121855:CACNA1G protein, human; D020747:Calcium Channels, T-Type; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002045:Bupivacaine