Magnesium Ascorbyl Phosphate Regulates the Expression of Inflammatory Biomarkers in Cultured Sebocytes.
BACKGROUND
Acne is an inflammatory skin disorder caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects.


OBJECTIVE
We performed this study to evaluate the effect of MAP on the expression of inflammatory biomarkers in cultured sebocytes.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay were performed for inflammatory cytokines and matrix metalloproteinases (MMPs) before and after treatment of cultured sebocytes with MAP (10(-2) M), lipopolysaccharide (LPS) (5 µg/ml) and a combination of MAP and LPS. RT-PCR and western blotting were also performed for antimicrobial peptides (AMPs) and Toll-like receptor (TLR)-4 before and after treatment of cultured sebocytes with MAP, LPS, and a combination of MAP and LPS. Quantification of lipid peroxidation was also conducted.


RESULTS
The increased expression of inflammatory cytokines after treatment of cultured sebocytes with LPS was decreased after treatment with MAP. MMPs, AMPs, and TLR-4 were decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS, and increased after treatment of cultured sebocytes with LPS alone. Lipid peroxidation was significantly decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS. MAP decreased the increased lipid peroxidation after treatment of cultured sebocytes with LPS.


CONCLUSIONS
MAP may be an effective alternative agent to improve inflammatory reactions in acne.
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
Weon Ju
WJ
Lee
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
Sang Lim
SL
Kim
Park & Lee Skin Clinic, Daegu, Korea.
Yoon Seok
YS
Choe
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
Yong Hyun
YH
Jang
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
Seok-Jong
SJ
Lee
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
Do Won
DW
Kim
Acne vulgaris; Ascorbate-2-phosphate; Cytokines; Lipopolysaccharides; Sebocytes