TORC1 activity is partially reduced under nitrogen starvation conditions in sake yeast Kyokai no. 7, Saccharomyces cerevisiae.
Industrial yeasts are generally unable to sporulate but treatment with the immunosuppressive drug rapamycin restores this ability in a sake yeast strain Kyokai no. 7 (K7), Saccharomyces cerevisiae. This finding suggests that TORC1 is active under sporulation conditions. Here, using a reporter gene assay, Northern and Western blots, we tried to gain insight into how TORC1 function under nitrogen starvation conditions in K7 cells. Similarly to a laboratory strain, RPS26A transcription was repressed and Npr1 was dephosphorylated in K7 cells, indicative of the expected loss of TORC1 function under nitrogen starvation. The expression of nitrogen catabolite repression-sensitive genes, however, was not induced, the level of Cln3 remained constant, and autophagy was more slowly induced than in a laboratory strain, all suggestive of active TORC1. We conclude that TORC1 activity is partially reduced under nitrogen starvation conditions in K7 cells.
Department of Biotechnology, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidoubata-Nishi, Shimoshinjyo-Nakano, Akita-shi, Akita Prefecture 010-0195, Japan. Electronic address: nnakazawa@akita-pu.ac.jp.
Nobushige
N
Nakazawa
Department of Biotechnology, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidoubata-Nishi, Shimoshinjyo-Nakano, Akita-shi, Akita Prefecture 010-0195, Japan.
Aya
A
Sato
Department of Biotechnology, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidoubata-Nishi, Shimoshinjyo-Nakano, Akita-shi, Akita Prefecture 010-0195, Japan.
Masahiro
M
Hosaka
D000434:Alcoholic Beverages / Q000382:microbiology*; D001343:Autophagy / Q000187:drug effects; D057465:Catabolite Repression / Q000235:genetics; D016213:Cyclins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D000076222:Mechanistic Target of Rapamycin Complex 1; D046912:Multiprotein Complexes / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism* / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology; D013172:Spores, Fungal / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
C079895:CLN3 protein, S cerevisiae; D016213:Cyclins; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; C066958:NPR1 protein, S cerevisiae; D011494:Protein Kinases; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases; D009584:Nitrogen; D020123:Sirolimus
Autophagy; Cln3; Nitrogen catabolite repression; Sake yeasts; TORC1 activity