Simultaneous determination of multiple androgens in mice organs with liquid chromatography tandem mass spectrometry.
Prostate cancer (PCa) is the most commonly diagnosed cancer in men worldwide. It is essentially dependent on potent androgens, such as testosterone (T) and dihydrotestosterone (DHT). The precursors of T and DHT, which includes androstenedione (A4) and dihydroepiandrosterone (DHEA), and also the metabolites of DHT, 5α-androstane-3α,17β-diol (3α-Diol) and 5α-androstane-3β,17β-diol (3β-Diol) are able to affect the development of PCa. Therefore, it is important to simultaneously determine all these key androgens. This study aims to develop and validate an LC-MS/MS quantification method to simultaneously detect and quantify the six related androgens, including T, DHT, A4, DHEA, 3α-Diol, and 3β-Diol in limited sample volume. The sample preparation involved liquid extraction with methyl tert-butyl ether (MTBE), following by chemical derivatisation with hydroxylamine. The limits of quantitation for T, DHT, A4, and DHEA were 0.05nM and 3α-Diol and 3β-Diol were 0.5nM with S/N ratio of at least 5:1 by using 100μL samples.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, PR China; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, 119074 Singapore, Singapore.
Shu Fang
SF
Soh
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, PR China.
Xiaoxing
X
Yin
Jiangsu Nhwa Pharmaceutical Corporation Ltd., 69 Minzhu South Road, Xuzhou 221009, PR China.
Jiaquan
J
Sun
Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, 119074 Singapore, Singapore.
Jun
J
Li
Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, 119074 Singapore, Singapore.
Eu Leong
EL
Yong
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, PR China. Electronic address: weiqunli@126.com.
Qunli
Q
Wei
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, PR China. Electronic address: gongyinhan@hotmail.com.
Yinhan
Y
Gong
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D002853:Chromatography, Liquid / Q000379:methods*; D003474:Curcumin / Q000008:administration & dosage / Q000031:analogs & derivatives; D019811:Hydroxylamine / Q000737:chemistry; D059625:Liquid-Liquid Extraction; D008297:Male; D008738:Methyl Ethers / Q000737:chemistry; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D000728:Androgens; D000970:Antineoplastic Agents; D008738:Methyl Ethers; C521105:dimethoxycurcumin; C043243:methyl tert-butyl ether; D019811:Hydroxylamine; D003474:Curcumin
Androgens; Castration resistant prostate cancer; Liquid chromatography tandem mass spectrometry