A plasma protein corona enhances the biocompatibility of Au@Fe3O4 Janus particles.
Au@Fe3O4 Janus particles (JPs) are heteroparticles with discrete domains defined by different materials. Their tunable composition and morphology confer multimodal and versatile capabilities for use as contrast agents and drug carriers in future medicine. Au@Fe3O4 JPs have colloidal properties and surface characteristics leading to interactions with proteins in biological fluids. The resulting protein adsorption layer ("protein corona") critically affects their interaction with living matter. Although Au@Fe3O4 JPs displayed good biocompatibility in a standardized in vitro situation, an in-depth characterization of the protein corona is of prime importance to unravel underlying mechanisms affecting their pathophysiology and biodistribution in vitro and in vivo. Here, we comparatively analyzed the human plasma corona of Au-thiol@Fe3O4-SiO2-PEG JPs (NH2-functionalized and non-functionalized) and spherical magnetite (Fe3O4-SiO2-PEG) particles and investigated its effects on colloidal stability, biocompatibility and cellular uptake. Label-free quantitative proteomic analyses revealed that complex coronas including almost 180 different proteins were formed within only one minute. Remarkably, in contrast to spherical magnetite particles with surface NH2 groups, the Janus structure prevented aggregation and the adhesion of opsonins. This resulted in an enhanced biocompatibility of corona sheathed JPs compared to spherical magnetite particles and corona-free JPs.
Institute of Diagnostic and Interventional Radiology, Division of Experimental Radiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany.
Lisa
L
Landgraf
Institute of Diagnostic and Interventional Radiology, Division of Experimental Radiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany.
Carolin
C
Christner
Institute for Immunology, University Medical Center of Mainz, Langenbeckstraße 1, D-55101 Mainz, Germany.
Wiebke
W
Storck
Institute for Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg University, Duesbergweg 10-14, D-55128 Mainz, Germany.
Isabel
I
Schick
Medical Physics Group, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich Schiller University Jena, Philosophenweg 3, D-07743 Jena, Germany.
Ines
I
Krumbein
Institute of Diagnostic and Interventional Radiology, Division of Experimental Radiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany.
Heidi
H
Dähring
Institute of Diagnostic and Interventional Radiology, Division of Experimental Radiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany.
Katja
K
Haedicke
Medical Physics Group, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich Schiller University Jena, Philosophenweg 3, D-07743 Jena, Germany.
Karl
K
Heinz-Herrmann
Institute of Diagnostic and Interventional Radiology, Division of Experimental Radiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany.
Ulf
U
Teichgräber
Medical Physics Group, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich Schiller University Jena, Philosophenweg 3, D-07743 Jena, Germany.
Jürgen R
JR
Reichenbach
Institute for Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg University, Duesbergweg 10-14, D-55128 Mainz, Germany.
Wolfgang
W
Tremel
Institute for Immunology, University Medical Center of Mainz, Langenbeckstraße 1, D-55101 Mainz, Germany. Electronic address: tenzer@uni-mainz.de.
Stefan
S
Tenzer
Institute of Diagnostic and Interventional Radiology, Division of Experimental Radiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany. Electronic address: ingrid.hilger@med.uni-jena.de.
Ingrid
I
Hilger
D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D003287:Contrast Media / Q000138:chemical synthesis; D042783:Endothelial Cells / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D064847:Multimodal Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D013499:Surface Properties; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed / Q000379:methods
D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D006046:Gold
In vivo imaging; Janus particles; Nanomedicine; Nanotoxicity; Protein corona