In ovo exposure to organophosphorous flame retardants: survival, development, neurochemical, and behavioral changes in white leghorn chickens.
Organophosphorous flame retardants (OPFRs) are contaminants of emerging concern. There is growing evidence of environmental contamination and exposures to both humans and wildlife. Here, the objective was to increase understanding of the potential neurodevelopmental effects of two relevant OPFRs, TMPP (tri (methylphenyl) phosphate; a non-halogen-containing OPFR) and TDCIPP (tris (1,3-dichloro-isopropyl) phosphate; a halogen-containing OPFR) in an avian embryo/chick model. We injected white leghorn chicken eggs with a range of TMPP (0, 10, 100, and 1000 ng/g) or TDCIPP (0, 10, 100, 1000, 50,000 ng/g) concentrations at incubation day 0 exposing embryos throughout the ~21-day in ovo period. Hatching success was unaffected by TMPP, but TDCIPP-exposed chicks had higher early-incubation mortality in 100 and 50,000 ng/g groups. On 7-9-day-old chicks, we assessed behavior via tests concerning righting reflex, angled balance beams, gait patterns, wing flap reflex, and open field movements. Chicks exposed to 100 ng/g TDCIPP achieved 40% lower maximum velocity in the open field test than vehicle-exposed controls, while those exposed to 1000 ng/g TDCIPP achieved 20% higher maximum velocity than vehicle-exposed controls. Chicks exposed to 50,000 ng/g TDCIPP showed reduced righting response success. There were no dose- or treatment-related differences in angled beam, gait analysis, or wing flap reflex tests. Cerebrum hemispheres from 10-day-old chicks were examined for neurochemistry (acetylcholinesterase [AChE] activity and both nicotinic [nACh] and muscarinic [mACh] acetylcholine receptor levels) and cerebellums were examined for histopathology. TDCIPP-exposed chicks had reduced number of degenerate Purkinje cells (TDCIPP, 1000 ng/g), possibly indicating disruption of neurodevelopment. No neurochemical effects were found in TMPP- or TDCIPP-exposed chicks. In general this study shows some possible neurodevelopmental effects in chicks exposed to TDCIPP when levels greatly exceeded those measured in wild bird eggs and no clear changes in TMPP-exposed chicks. This study builds upon previous in vitro studies as well as work on adult birds showing that toxic responses in avian models can vary among species and OPFRs.
Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, USA; Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Mark
M
Bradley
Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, USA; ToxServices, Ann Arbor, MI, USA.
Jennifer
J
Rutkiewicz
Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, USA; Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Krittika
K
Mittal
Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, QC, Canada; Ecotoxicology and Wildlife Health Division, Science and Technology Branch, Environment Canada, Burlington, ON, Canada.
Kimberly
K
Fernie
Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, USA; Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, QC, Canada. Electronic address: niladri.basu@mcgill.ca.
Niladri
N
Basu
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D054022:Cerebrum / Q000187:drug effects* / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D047108:Embryonic Development / Q000187:drug effects*; D005411:Flame Retardants / Q000633:toxicity*; D009043:Motor Activity / Q000187:drug effects*; D009943:Organophosphorus Compounds / Q000633:toxicity*; D011689:Purkinje Cells / Q000187:drug effects* / Q000473:pathology*; D011976:Receptors, Muscarinic / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism; D012018:Reflex / Q000187:drug effects
D005411:Flame Retardants; D009943:Organophosphorus Compounds; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D000110:Acetylcholinesterase
Avian toxicology; Behavior; Birds; Development; Flame retardants; Neurotoxicology