Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique.
The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. mariafe.laguna@upm.es.|Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2. 28006 Madrid, Spain. mariafe.laguna@upm.es.
Maríafe
M
Laguna
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. m.holgado@upm.es.|Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2. 28006 Madrid, Spain. m.holgado@upm.es.
Miguel
M
Holgado
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. ana.lopez@upm.es.
Ana L
AL
Hernandez
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. beatriz.santamaria@ctb.upm.es.|BioOpticalDetection, Centro de Empresas de la UPM, Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. beatriz.santamaria@ctb.upm.es.
Beatriz
B
Santamaría
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. alavin@etsii.upm.es.|Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2. 28006 Madrid, Spain. alavin@etsii.upm.es.
Alvaro
A
Lavín
Bioftalmik. Parque Tecnológico Zamudio Ed. 800 2ª Planta 48160, Bizkaia, Spain. javier.soria@bioftalmik.com.
Javier
J
Soria
Bioftalmik. Parque Tecnológico Zamudio Ed. 800 2ª Planta 48160, Bizkaia, Spain. tatiana.suarez@bioftalmik.com.
Tatiana
T
Suarez
AntibodyBcn, MRB 104 Modul b UAB Campus, 08193 Bellaterra, Barcelona, Spain. carlota.bardina@antibodybcn.com.
Carlota
C
Bardina
AntibodyBcn, MRB 104 Modul b UAB Campus, 08193 Bellaterra, Barcelona, Spain. monica.jara@antibodybcn.com.
Mónica
M
Jara
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. fj.sanza@upm.es.|BioOpticalDetection, Centro de Empresas de la UPM, Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. fj.sanza@upm.es.
Francisco J
FJ
Sanza
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain. rafael.casquel@upm.es.|Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2. 28006 Madrid, Spain. rafael.casquel@upm.es.
Rafael
R
Casquel
D000818:Animals; D017302:Annexins / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000915:Antibody Affinity / Q000276:immunology*; D000941:Antigens / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D015352:Dry Eye Syndromes / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D007700:Kinetics; D008807:Mice, Inbred BALB C; D055620:Optical Phenomena; D013194:Staining and Labeling
D017302:Annexins; D000911:Antibodies, Monoclonal; D000941:Antigens; D015415:Biomarkers
biosensors; dry eye biomarkers; label-free immunoassay; photonic sensing cells BICELLs