Presence of antibodies to Salmonella in tuatara (Sphenodon punctatus) sera.
Colonisation of a host by pathogenic microorganisms is a near constant threat to the health of all vertebrates and most species have evolved an efficient adaptive immune response which produces antibodies following exposure to a specific antigen. The strength of this response can be influenced by many factors including sex and season. Tuatara are exposed to Salmonella through contact with infected skinks and soil; however, no gastrointestinal colonisation of tuatara with Salmonella has been found. Using Western blot and flow cytometry we have demonstrated that tuatara possess antibodies which recognise Salmonella antigens, but many of these antibodies are not specific and are cross-reactive with two closely related and ubiquitous bacteria, Escherichia coli and Citrobacter koseri. Our study describes the anti-Salmonella immune responses in tuatara and will help to inform decisions around maintaining wildlife health, as well as providing important insights into the role and development of adaptive immunity in reptilian species.
Allan Wilson Centre for Molecular Ecology and Evolution, Victoria University of Wellington, P.O. Box 600, Wellington, New Zealand. Electronic address: d.middleton@auckland.ac.nz.
Danielle M R L
DM
Middleton
Allan Wilson Centre for Molecular Ecology and Evolution, Victoria University of Wellington, P.O. Box 600, Wellington, New Zealand.
Nicola J
NJ
Nelson
Wildbase, Institute of Veterinary, Animal and Biomedical Science, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
Brett D
BD
Gartrell
Allan Wilson Centre for Molecular Ecology and Evolution, Victoria University of Wellington, P.O. Box 600, Wellington, New Zealand.
Anne C
AC
La Flamme
D056704:Adaptive Immunity; D000818:Animals; D000835:Animals, Wild / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D015153:Blotting, Western; D041041:Citrobacter koseri / Q000276:immunology; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology; D008116:Lizards / Q000276:immunology* / Q000382:microbiology; D012475:Salmonella / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Antibody; Flow cytometry; Immune response; Salmonella; Tuatara; Western blot