[Effect of cancer-associated fibroblasts on proliferation and invasion of gallbladder carcinoma cells].
OBJECTIVE
To investigate the effect of cancer-associated fibroblasts (CAFs) on the growth and invasion of gallbladder cancer cells.


METHODS
The CAFs were isolated from human primary gallbladder carcinoma tissues by tissue culture and digestion methods. The cells were purified by differential adhesion method, and the primary cells were identified morphologically and immunocytochemically. The proliferation and invasion of two human gallbladder carcinoma cell lines (SBC-996 and GBC-SD) co-cultured with CAFs were detected by MTT and Transwell chamber assays.


RESULTS
Gallbladder carcinoma CAFs were isolated successfully by both tissue culture and enzyme digestion methods, and the latter method was more convenient and efficient. MTT and Transwell assays showed that CAFs significantly promoted the proliferation and invasion of the two gallbladder carcinoma cell lines.


CONCLUSIONS
CAFs can promote the proliferation and invasion of gallbladder carcinoma cells in vitro, suggesting the important role of CAFs in the development of gallbladder carcinoma.
Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. E-mail: docchenchen@163.com.
Chen
C
Chen
Haoxin
H
Shen
Jie
J
Tao
Huwei
H
Song
Li
L
Ma
Lin
L
Wang
Zhimin
Z
Geng
D002277:Carcinoma / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology*; D005706:Gallbladder Neoplasms / Q000473:pathology*; D006801:Humans