Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins.
OBJECTIVE
The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity.


METHODS
Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot.


RESULTS
PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05).


CONCLUSIONS
This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins.
Department of Physical Education, University of Las Palmas de Gran Canaria, Campus Universitario de Tafira, 35017, Las Palmas de Gran Canaria, Canary Islands, Las Palmas, Spain. jesus.ponce.gonzalez@gmail.com.
Jesús Gustavo
JG
Ponce-González
GENUD Toledo Research Group, University of Castilla-La Mancha, Toledo, Spain.|Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
Ignacio
I
Ara
Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
Steen
S
Larsen
Department of Physical Education, University of Las Palmas de Gran Canaria, Campus Universitario de Tafira, 35017, Las Palmas de Gran Canaria, Canary Islands, Las Palmas, Spain.|Research Institute of Biomedical and Health Sciences (IUIBS), Las Palmas de Gran Canaria, Canary Islands, Spain.
Borja
B
Guerra
Department of Physical Education, University of Las Palmas de Gran Canaria, Campus Universitario de Tafira, 35017, Las Palmas de Gran Canaria, Canary Islands, Las Palmas, Spain.|Research Institute of Biomedical and Health Sciences (IUIBS), Las Palmas de Gran Canaria, Canary Islands, Spain.
Jose A L
JA
Calbet
Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
Jørn Wulff
JW
Helge
D050154:Adiposity / Q000502:physiology*; D000328:Adult; D006801:Humans; D008297:Male; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D009765:Obesity / Q000378:metabolism*; D001678:Organelle Biogenesis; D052097:Quadriceps Muscle / Q000378:metabolism
D024101:Mitochondrial Proteins
Fat oxidation; Mitochondrial biogenesis; Regional differences and obesity