Modulation of Cholesterol-Related Gene Expression by Dietary Fiber Fractions from Edible Mushrooms.
Mushrooms are a source of dietary fiber (DF) with a cholesterol-lowering effect. However, their underlying mechanisms are poorly understood. The effect of DF-enriched fractions from three mushrooms species on cholesterol-related expression was studied in vitro. The Pleurotus ostreatus DF fraction (PDF) was used in mice models to assess its potential palliative or preventive effect against hypercholesterolemia. PDF induced a transcriptional response in Caco-2 cells, suggesting a possible cholesterol-lowering effect. In the palliative setting, PDF reduced hepatic triglyceride likely because Dgat1 was downregulated. However, cholesterol-related biochemical data showed no changes and no relation with the observed transcriptional modulation. In the preventive setting, PDF modulated cholesterol-related genes expression in a manner similar to that of simvastatin and ezetimibe in the liver, although no changes in plasma and liver biochemical data were induced. Therefore, PDF may be useful reducing hepatic triglyceride accumulation. Because it induced a molecular response similar to hypocholesterolemic drugs in liver, further dose-dependent studies should be carried out.
Department of Experimental Surgery, Research Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
Víctor
V
Caz
Department of Production and Characterization of Novel Foods, CIAL - Research Institute in Food Science (UAM+CSIC), Universidad Autónoma de Madrid , C/Nicolas Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
Alicia
A
Gil-Ramírez
Department of Experimental Surgery, Research Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
Carlota
C
Largo
Department of Experimental Surgery, Research Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
María
M
Tabernero
Department of Experimental Surgery, Research Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
Mónica
M
Santamaría
IMDEA Food Institute , Pabellón Central del Antiguo Hospital de Cantoblanco (Edificio no. 7), Carretera de Cantoblanco no. 8, 28049 Madrid, Spain.
Roberto
R
Martín-Hernández
Department of Production and Characterization of Novel Foods, CIAL - Research Institute in Food Science (UAM+CSIC), Universidad Autónoma de Madrid , C/Nicolas Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
Francisco R
FR
Marín
Department of Production and Characterization of Novel Foods, CIAL - Research Institute in Food Science (UAM+CSIC), Universidad Autónoma de Madrid , C/Nicolas Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.|IMDEA Food Institute , Pabellón Central del Antiguo Hospital de Cantoblanco (Edificio no. 7), Carretera de Cantoblanco no. 8, 28049 Madrid, Spain.
Guillermo
G
Reglero
Department of Production and Characterization of Novel Foods, CIAL - Research Institute in Food Science (UAM+CSIC), Universidad Autónoma de Madrid , C/Nicolas Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
Cristina
C
Soler-Rivas
D000364:Agaricus / Q000737:chemistry; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D018938:Caco-2 Cells / Q000187:drug effects; D002784:Cholesterol / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D004043:Dietary Fiber / Q000494:pharmacology*; D019587:Dietary Supplements; D005243:Feces; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006937:Hypercholesterolemia / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects; D008297:Male; D008810:Mice, Inbred C57BL; D020076:Pleurotus / Q000737:chemistry*; D020942:Shiitake Mushrooms / Q000737:chemistry
D004043:Dietary Fiber; D002784:Cholesterol
Agaricus bisporus; Lentinus edodes; Pleurotus ostreatus; cholesterol; dietary fibers; low-density array (LDA); oyster mushroom; polysaccharides; shiitake mushroom; white button mushroom; β-glucans