An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria.
OBJECTIVE
The purpose of this study was to evaluate the antibacterial ability of three MTA (MTA-Angelus, Endocem MTA, and ProRoot MTA) against five typical oral bacteria (Streptococcus mutans, Enterococcus faecalis, Lactobacillus rhamnosus, Lactobacillus paracasei, and Porphyromonas gingivalis).


METHODS
For disc diffusion test, each test material was placed into agar plates after inoculation of each bacterial strain. The zones of inhibition of bacterial growth were then measured. Antibacterial broth test was performed by adding the test material into the media. Colony-forming units were counted after incubation with bacteria. The data were analyzed using ANOVA and the Tukey's test.


RESULTS
Disc diffusion test showed that the antibacterial activity against S. mutans, L. rhamnosus, L. paracasei, and P. gingivalis ranked in decreasing order of MTA-Angelus>ProRoot MTA>Endocem MTA (p<0.05). An inhibitory effect against E. faecalis was only observed in Endocem MTA. Antibacterial broth test showed that the antibacterial activity against all bacteria was Endocem MTA>MTA-Angelus>ProRoot MTA (p<0.05).


CONCLUSIONS
Discrepant results were obtained from the disc diffusion and antibacterial broth test, with MTA-Angelus and Endocem MTA being most effective, respectively. Both tests revealed that the most resistant bacteria was E. faecalis, which was not susceptible at all, except to Endocem MTA in disc diffusion test.
Department of Conservative Dentistry, Seoul National University Gwanak Dental Hospital, Seoul, South Korea.
Ryan Jin-Young
RJ
Kim
Postgraduate student, Korea University University Graduate School of Medicine, Seoul, South Korea.
Myung-Ook
MO
Kim
Institute for Clinical Dental Research, Korea University Guro Hospital, Seoul, South Korea.
Ki-Sun
KS
Lee
Department of Orthodontics, Korea University Guro Hospital, Seoul, South Korea.
Dong-Yul
DY
Lee
Department of Conservative Dentistry, Korea University Guro Hospital, 97 Gurodong-Gil, Guro-Gu, Seoul, South Korea. Electronic address: endoshin11@gmail.com.
Joo-Hee
JH
Shin
D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology*; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001729:Bismuth / Q000494:pharmacology*; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003738:Dental Cements / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects*; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000494:pharmacology; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects*
D017607:Aluminum Compounds; D000900:Anti-Bacterial Agents; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; C475480:MTA-Angelus; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C586519:endocem; C086631:mineral trioxide aggregate; D001729:Bismuth
Base; Disc diffusion test; Endodontics; MTA; Pulp capping; Zone of inhibition