Metallic Profile of Whole Blood and Plasma in a Series of 99 Healthy Children.
In recent years, special emphasis has been put on heavy metals. Children are very sensitive to accumulation of metals. Furthermore, as regards elements, the reference values in children are scarce in the literature as it is difficult to obtain the large quantity of blood necessary to analyze many metals by the conventional atomic absorption spectrometry technique. An inductively coupled plasma mass spectrometry (ICP-MS) procedure that uses a reduced sample of 0.3 mL whole blood or plasma is adapted to multielemental determinations. We applied a previously validated technique for adults that simultaneously quantifies 25 elements by ICP-MS in whole blood and 23 in plasma in a series of 99 healthy children ranging from under 5 years to <18 years, without exposure to metal or drug-containing metals. The aims of the study were to compare metallic concentrations according to the age among children and metallic concentration differences between children and adults. The blood and plasma pediatric metallic profile is a practical useful tool for many purposes in clinical toxicology, forensic toxicology and any cases of metal environmental exposure.
Laboratoire de Toxicologie, ABTE ToxEMAC EA 4651, Faculté de Médecine et de Pharmacie, 22 boulevard Gambetta, 76183 Rouen Cedex 1, France Laboratoire de Pharmacocinétique et de Toxicologie Cliniques, Groupe Hospitalier du Havre, BP 24, 76083 Le Havre Cedex, France jean-pierre.goulle@univ-rouen.fr.
Jean-Pierre
JP
Goullé
Service de Pédiatrie, Groupe Hospitalier du Havre, BP 24, 76083 Le Havre Cedex, France.
Pascal
P
Le Roux
Département de Pédiatrie et Centre d'Investigation Clinique-Inserm 204, Hôpitaux de Rouen, 1 rue de Germont, 76031 Rouen Cedex, France.
Mireille
M
Castanet
Laboratoire de Pharmacocinétique et de Toxicologie Cliniques, Groupe Hospitalier du Havre, BP 24, 76083 Le Havre Cedex, France.
Loïc
L
Mahieu
Service de Pédiatrie, Groupe Hospitalier du Havre, BP 24, 76083 Le Havre Cedex, France.
Stéphanie
S
Guyet-Job
Laboratoire de Toxicologie, ABTE ToxEMAC EA 4651, Faculté de Médecine et de Pharmacie, 22 boulevard Gambetta, 76183 Rouen Cedex 1, France.
Michel
M
Guerbet
D000293:Adolescent; D001774:Blood Chemical Analysis; D002648:Child; D002675:Child, Preschool; D005260:Female; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D008297:Male; D019216:Metals, Heavy / Q000097:blood*; D010949:Plasma / Q000737:chemistry*; D012016:Reference Values; D013054:Spectrophotometry, Atomic
D019216:Metals, Heavy