The impact of the presence of antithyroid antibodies on pregnancy outcome following intracytoplasmatic sperm injection-ICSI and embryo transfer in women with normal thyreotropine levels.
OBJECTIVE
The aim of our study was to investigate the impact of anti-thyroid peroxidase antibodies (Anti-TPO) on pregnancy outcome following the in vitro fertilization and embryo transfer (IVF-ET) in general groups and in subgroups divided according to AMH level and age.


METHODS
A total of 114 patients positive for anti-thyroid peroxidase antibodies (Anti-TPO+ group) and 495 infertile women negative for anti-thyroid peroxidase antibodies (Anti-TPO- group) undergoing IVF with ICSI from April 2010 to April 2012 were analyzed retrospectively.


RESULTS
There were no significant differences in age, BMI, basal FSH, LH, AMH levels and duration of infertility between the two main groups. No significant differences in terms of the days of ovarian stimulation, estradiol level in day 8, total gonadotropin dose, number of oocytes retrieved, available embryos and blastocysts, number of embryos transferred nor in rates of fertilization, implantation, clinical pregnancy, live birth and abortion rate between two main groups were found. The only statistically significant difference among the groups with different anti-TPO antibodies levels was found in basal FSH concentration and BMI. Among the clinical outcomes of IVF with respect to the different anti-TPO levels, the only significant difference was found for the number of oocytes retrieved. Analysis of the baseline parameters in relationship to age categories and AMH levels found significant differences between women positive and negative for thyroid antibodies with respect to basal FSH and LH levels for women >37 years and for basal FSH in AMH <0.6 subgroup.


CONCLUSIONS
The present study reveals that patients with anti-TPO antibodies showed no significant differences in fertilization, implantation, pregnancy rates, live birth rates and no higher risk for miscarriage following IVF-ET when compared with those negative for anti-thyroid antibodies.
Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdańsk, Gdańsk, Poland.|INVICTA Fertility and Reproductive Center, Gdańsk, Poland.|INVICTA Fertility and Reproductive Center, Invicta Złota 6 Str, 00019, Warsaw, Poland.
K
K
Łukaszuk
INVICTA Fertility and Reproductive Center, Invicta Złota 6 Str, 00019, Warsaw, Poland. mkunicki@op.pl.
M
M
Kunicki
Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdańsk, Gdańsk, Poland.
P
P
Kulwikowska
INVICTA Fertility and Reproductive Center, Gdańsk, Poland.
J
J
Liss
Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdańsk, Gdańsk, Poland.|INVICTA Fertility and Reproductive Center, Gdańsk, Poland.
E
E
Pastuszek
Department of Chemical and Process Engineering Chemical Faculty, Gdansk University of Technology, Gdańsk, Poland.
M
M
Jaszczołt
Department of Gynecological Endocrinology, Poznan Univeristy of Medcial Sciences, Poznan, Poland.
B
B
Męczekalski
Institute of Computer Science, Faculty of Mathematics, Physics and Informatics, University of Gdansk, Gdańsk, Poland.
K
K
Skowroński
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology*; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood* / Q000628:therapy*; D007453:Iodide Peroxidase / Q000276:immunology*; D033862:Iron-Binding Proteins / Q000276:immunology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D013972:Thyrotropin / Q000097:blood*
D001323:Autoantibodies; D001324:Autoantigens; D033862:Iron-Binding Proteins; D013972:Thyrotropin; C056658:TPO protein, human; D007453:Iodide Peroxidase
Anti-thyroid antibodies; Intracytoplasmatic sperm iniection-ICSI; Miscarriage; TSH