Toxic effects of magnesium oxide nanoparticles on early developmental and larval stages of zebrafish (Danio rerio).
Magnesium oxide nanoparticles (MgONPs) are used in medicine, manufacturing and food industries. Because of their extensive application in our daily lives, environmental exposure to these nanoparticles is inevitable. The present study examined the effects of MgONPs on zebrafish (Danio rerio) early developmental stages. The results showed that, at different concentrations, MgONPs induced cellular apoptosis and intracellular reactive oxygen species. The hatching rate and survival of embryos decreased in a dose dependent manner. The 96-h LC50 value of MgONPs on zebrafish survival was 428 mg/l and the 48-h EC50 value of MgONPs on zebrafish embryo hatching rate was 175 mg/l. Moreover different types of malformation were observed in exposed embryos. The results demonstrate the toxic effects of MgONPs on zebrafish embryos and emphasize the need for further studies.
Department of Developmental Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. Electronic address: m54biology@yahoo.com.
Mehdi
M
Ghobadian
Department of Developmental Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Mohammad
M
Nabiuni
Department of Developmental Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Kazem
K
Parivar
Department of Biochemistry, Zanjan University of Medical Sciences, Zanjan, Iran.
Mojtaba
M
Fathi
Department of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Jamileh
J
Pazooki
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D047108:Embryonic Development / Q000187:drug effects*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008277:Magnesium Oxide / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015027:Zebrafish / Q000254:growth & development*
D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D008277:Magnesium Oxide
Fish embryos; Malformation; MgO nanoparticles; Toxicity; Zebrafish