Metabolic control analysis of L-lactate synthesis pathway in Rhizopus oryzae As 3.2686.
The relationship between the metabolic flux and the activities of the pyruvate branching enzymes of Rhizopus oryzae As 3.2686 during L-lactate fermentation was investigated using the perturbation method of aeration. The control coefficients for five enzymes, pyruvate dehydrogenase (PDH), pyruvate carboxylase (PC), pyruvate decarboxylase (PDC), lactate dehydrogenase (LDH), and alcohol dehydrogenase (ADH), were calculated. Our results indicated significant correlations between PDH and PC, PDC and LDH, PDC and ADH, LDH and ADH, and PDC and PC. It also appeared that PDH, PC, and LDH strongly controlled the L-lactate flux; PDH and ADH strongly controlled the ethanol flux; while PDH and PC strongly controlled the acetyl coenzyme A flux and the oxaloacetate flux. Further, the flux control coefficient curves indicated that the control of the system gradually transferred from PDC to PC during the steady state. Therefore, PC was the key rate-limiting enzyme that controls the flux distribution.
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Wei
W
Ke
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Shu
S
Chang
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Xiaoju
X
Chen
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.|Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Shuizhong
S
Luo
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.|Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Shaotong
S
Jiang
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.|Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Peizhou
P
Yang
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.|Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China.
Xuefeng
X
Wu
School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China. zhengzhi@hfut.edu.cn.|Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, People's Republic of China. zhengzhi@hfut.edu.cn.
Zhi
Z
Zheng
D000105:Acetyl Coenzyme A / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics
D005656:Fungal Proteins; D019344:Lactic Acid; D000105:Acetyl Coenzyme A
Control coefficient; Flux control coefficient; Metabolic control analysis; Pyruvate branch point; Rhizopus oryzae