Cardamonin Regulates miR-21 Expression and Suppresses Angiogenesis Induced by Vascular Endothelial Growth Factor.
Cardamonin has promising potential in cancer prevention and therapy by interacting with proteins and modifying the expressions and activities, including factors of cell survival, proliferation, and angiogenesis. In our precious study, we have demonstrated that cardamonin suppressed vascular endothelial growth factor- (VEGF-) induced angiogenesis as evaluated in the mouse aortic ring assay. It is also known that microRNAs (miRNAs) play important roles in angiogenesis. Herein, we hypothesized whether antiangiogenesis effect of cardamonin in human umbilical vein endothelial cells (HUVECs) triggered by VEGF was associated with miRNAs. We found that cardamonin reduced the miR-21 expression induced by VEGF in HUVECs. Treatment with miR-21 mimics abolished the effects of cardamonin on VEGF-induced cell proliferation, migration, and angiogenesis in HUVECs. However, treatment with miR-21 inhibitors presented the opposite effects, indicating the vital role of miR-21 in this process. Our study provides a new insight of the preliminary mechanism of anti-VEGF-induced angiogenesis by cardamonin in HUVECs.
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Fu-Sheng
FS
Jiang
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Sha-Sha
SS
Tian
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau.
Jin-Jian
JJ
Lu
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Xing-Hong
XH
Ding
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Chao-Dong
CD
Qian
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Bin
B
Ding
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Zhi-Shan
ZS
Ding
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Bo
B
Jin
D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology / Q000627:therapeutic use*; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000235:genetics*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000009:adverse effects*
D047188:Chalcones; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; C436747:cardamonin