Different Dicer-like protein components required for intracellular and systemic antiviral silencing in Arabidopsis thaliana.
Eukaryotes employ RNA silencing as an innate defense system against invading viruses. Dicer proteins play the most crucial role in initiating this antiviral pathway as they recognize and process incoming viral nucleic acids into small interfering RNAs. Generally, 2 successive infection stages constitute viral infection in plants. First, the virus multiplies in initially infected cells or organs after viral transmission and then the virus subsequently spreads systemically through the vasculature to distal plant tissues or organs. Thus, antiviral silencing in plants must cope with both local and systemic invasion of viruses. In a recent study using 2 sets of different experiments, we clearly demonstrated the differential requirement for Dicer-like 4 (DCL4) and DCL2 proteins in the inhibition of intracellular and systemic infection by potato virus X in Arabidopsis thaliana. Taken together with the results of other studies, here we further discuss the functional specificity of DCL proteins in the antiviral silencing pathway.
a Institute of Plant Science and Resources (IPSR) ; Okayama University ; Kurashiki , Japan.
Ida Bagus
IB
Andika
Kazuyuki
K
Maruyama
Liying
L
Sun
Hideki
H
Kondo
Tetsuo
T
Tamada
Nobuhiro
N
Suzuki
D017360:Arabidopsis / Q000378:metabolism* / Q000821:virology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D060467:Disease Resistance; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000235:genetics* / Q000472:pathogenicity; D034622:RNA Interference; D034741:RNA, Small Interfering; D043244:Ribonuclease III / Q000378:metabolism*
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; C504982:DCL4 protein, Arabidopsis; C511919:DCL2 protein, Arabidopsis; D043244:Ribonuclease III
Dicer-like protein; RNA silencing; antiviral defense, Arabidopsis thaliana; potato virus X