Knockdown of Nestin inhibits proliferation and migration of colorectal cancer cells.
Nestin, a member of type VI intermediate filament protein family, is widely expressed in mammalian nervous tissue and stem/precursor cells of non-neuronal normal tissues. Nestin has also been investigated to determine possible tumor-promoting functions. However, whether Nestin is involved in colorectal cancer (CRC) cells remains unclear. In this report, Nestin expression was upregulated in stromal cells of human CRC tissues. Endogenous Nestin expression in CRC cell lines SW480 and HCT116 was knocked down by a lentivirus. MTT and colony formation assays revealed that Nestin deletion significantly inhibits the proliferation of CRC cell lines; flow cytometer analysis showed that Nestin deletion causes cell cycle arrest at S phase. Transwell chamber and wound healing scratch assays also revealed that Nestin deletion suppresses cell migration. Our findings indicated that Nestin plays an essential role in CRC progression; thus, Nestin can be applied as a therapeutic target of CRC.
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China ; Department of Medical Laboratory, Shanghai 10th People's Hospital of Tongji University Shanghai 200072, P. R. China.
Jing
J
Li
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China ; Department of Medical Laboratory, Shanghai 10th People's Hospital of Tongji University Shanghai 200072, P. R. China.
Rui
R
Wang
Department of General Surgery, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China.
Lei
L
Yang
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China ; Department of Medical Laboratory, Shanghai 10th People's Hospital of Tongji University Shanghai 200072, P. R. China.
Qi
Q
Wu
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China.
Qinwan
Q
Wang
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China ; Department of Medical Laboratory, Shanghai 10th People's Hospital of Tongji University Shanghai 200072, P. R. China.
Zhengchao
Z
Nie
Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai TCM University Shanghai 200071, P. R. China.
Yongchun
Y
Yu
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China.
Ji
J
Ma
Central Laboratory, Shanghai 10th People's Hospital of Tongji University 200072, P. R. China.
Qiuhui
Q
Pan
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D064231:Nestin / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering; D014162:Transfection
C577484:NES protein, human; D064231:Nestin; D034741:RNA, Small Interfering
Nestin; colorectal cancer; metastasis