A conformational epitope mapped in the bovine herpesvirus type 1 envelope glycoprotein B by phage display and the HSV-1 3D structure.
The selected dodecapeptide (1)DRALYGPTVIDH(12) from a phage-displayed peptide library and the crystal structure of the envelope glycoprotein B (Env gB) from Herpes Simplex Virus type 1 (HSV-1) led us to the identification of a new discontinuous epitope on the Bovine herpesvirus type 1 (BoHV-1) Env gB. In silico analysis revealed a short BoHV-1 gB motif ((338)YKRD(341)) within a epitope region, with a high similarity to the motifs shared by the dodecapeptide N-terminal region ((5)YxARD(1)) and HSV-1 Env gB ((326)YARD(329)), in which the (328)Arg residue is described to be a neutralizing antibody target. Besides the characterization of an antibody-binding site of the BoHV-1 Env gB, we have demonstrated that the phage-fused peptide has the potential to be used as a reagent for virus diagnosis by phage-ELISA assay, which discriminated BoHV-1 infected serum samples from negative ones.
Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil; Laboratório de Virologia Animal, Departamento de Microbiologia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: greyci_almeid@hotmail.com.
Greyciele R
GR
Almeida
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. Electronic address: lrgoulart@ufu.br.
Luiz Ricardo
LR
Goulart
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. Electronic address: jair.cunha.junior@gmail.com.
Jair P
JP
Cunha-Junior
Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: bataus@ufg.br.
Luiz A M
LA
Bataus
Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil; Laboratório de Virologia Animal, Departamento de Microbiologia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: gre_vet@hotmail.com.
Greice
G
Japolla
Laboratório de Virologia Animal, Departamento de Microbiologia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: wilia@hotmail.com.
Wilia M E D
WM
Brito
Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: ivan@icb.ufg.br.
Ivan T N
IT
Campos
Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: cris.lino@gmail.com.
Cristina
C
Ribeiro
Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: grlino@gmail.com.
Guilherme R L
GR
Souza
D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000235:genetics*; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D008958:Models, Molecular; D009841:Oligonucleotides / Q000235:genetics; D019151:Peptide Library; D011487:Protein Conformation; D014764:Viral Proteins / Q000235:genetics*
D057134:Antibodies, Neutralizing; D000939:Epitopes; D009841:Oligonucleotides; D019151:Peptide Library; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins
BoHV-1; Discontinuous epitope; Epitope mapping; Glycoprotein B; Structural homology