Bacillus hisashiisp. nov., isolated from the caeca of gnotobiotic mice fed with thermophile-fermented compost.
A taxonomic study was performed on 15 bacterial isolates from the caeca of gnotobiotic mice that had been fed with thermophile-fermented compost. The 15 isolates were thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming bacteria, and were most closely related toBacillus thermoamylovoransCNCM I-1378T. The 16S rRNA gene sequence of strain N-11T, selected as representative of this new group, showed a similarity of 99.4 % withBacillus thermoamylovoransCNCM I-1378T, 94.7 % withBacillus thermolactisR-6488T, and 94.4 % withBacillus kokeshiiformisMO-04T. The isolates were then classified into two distinct groups based on a (GTG)5-fingerprint analysis. Two isolates, N-11Tand N-21, were the representatives of these two groups, respectively.` The N-11Tand N-21 isolates showed 66-71 % DNA-DNA relatedness with one other, but had less than 37 % DNA-DNA relatedness withB. thermoamylovoransLMG 18084T. The other 13 isolates showed DNA-DNA relatedness values above 74 % with the N-11Tisolate. All 15 isolates grew at 25-60 °C (optimum 50 °C), pH 6-8 (optimum pH 7) and were capable of growing on a medium containing 6 % (w/v) NaCl (optimum 0.5 %). The 15 isolates could be distinguished fromB. thermoamylovoransLMG 18084Tbecause they showed Tween 80 hydrolysis activity and did not produce acid from melibiose. The major fatty acids were anteiso-C15 : 0, C16 : 0, iso-C15 : 0, iso-C14 : 0and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and several unidentified phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan wasmeso-diaminopimelic acid. The menaquinone was MK-7. The DNA G+C content was 37.9 mol%. Based on the phenotypic properties, the 15 strains represent a novel species of the genusBacillus, for which the nameBacillus hisashiisp. nov. is proposed. The type strain is N-11T( = NRBC 110226T= LMG 28201T).
Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
Ayaka
A
Nishida
Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.|Department of Biochemistry and Integrative Medical Biology, Keio School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo 160-8582 Japan.|Japan Eco-science (Nikkan Kagaku) Co. Ltd, Shiomigaoka-cho 11-1-2F, Chuuou-ku, Chiba-city, Chiba 260-0034, Japan.|Miroku Co. Ltd, Iwaya 706-27, Kitsuki-city, Oita 873-0021, Japan.
Hirokuni
H
Miyamoto
Department of Molecular Virology, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-ku, Tokyo 113-8519, Japan.
Sankichi
S
Horiuchi
Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
Ryo
R
Watanabe
Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Hidetoshi
H
Morita
Institute for Advanced Biosciences, Keio University, 264-2 Mizukami, Kakuganji, Tsuruoka, Yamagata 997-0052, Japan.|Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Shinji
S
Fukuda
Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.|Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.|Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Hiroshi
H
Ohno
Research Center for Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Shizuko
S
Ichinose
Miroku Co. Ltd, Iwaya 706-27, Kitsuki-city, Oita 873-0021, Japan.
Hisashi
H
Miyamoto
Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
Hiroaki
H
Kodama