[Simultaneously preparation of grams of high purity tyrosol, crenulatin and salidroside from Rhodiola crenulata].
Tyrosol, crenulatin and salidroside are the main active constituents of Rhodiola crenulata, with extensive pharmacological activities. In the study, grams of high purity tyrosol, crenulatin and salidroside were simultaneously separated from R. crenulata by the first time. Firstly, R. crenulata was extracted by 70% alcohol. Then, with the yields of three compounds as the index, the macroporous resin was optimized. At last, grams of high purity tyrosol, crenulatin and salidroside were isolated by D-101 macroporousresin, purified by column chromatography. Detected by HPLC, the purity of three compounds were higher than 98%. This method has the advantages of simple process and operation, less dosage of organic solvent, highly yield and reproducibility, suitable for the simultaneously preparation of tyrosol, crenulatin and salidroside.
Xin
X
Luo
Xue-jing
XJ
Wang
Shi-ping
SP
Li
Qiao
Q
Zhang
Yi-wu
YW
Zhao
Huang Wen-zhe
Zhen-zhong
ZZ
Wang
Wei
W
Xiao
D005591:Chemical Fractionation / Q000379:methods*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D029771:Rhodiola / Q000737:chemistry*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C076945:crenulatin coumarin; C009172:rhodioloside; D010626:Phenylethyl Alcohol