Preparation of ATE1 Enzyme from Native Mammalian Tissues.
Early studies of protein arginylation preceded the wide use of recombinant protein expression and relied heavily on fractionation of proteins from native tissues. The procedure described below has been developed in 1970 by R. Soffer, in the wake of arginylation discovery in 1963. This chapter follows the detailed procedure originally published by R. Soffer in the 1970, adapted from his article in consultation with R. Soffer, H. Kaji, and A. Kaji.
Department of Animal Biology School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, 19104, USA, akashina@vet.upenn.edu.
Anna S
AS
Kashina
D019881:Aminoacyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002845:Chromatography / Q000379:methods; D006801:Humans; D008322:Mammals; D013347:Subcellular Fractions
D019881:Aminoacyltransferases; C019727:arginyltransferase