Up-regulated miR-155-5p promotes cell proliferation, invasion and metastasis in colorectal carcinoma.
OBJECTIVE
Emerging evidences indicate that miR-155-5p is associated with some cancer tumorigenesis, but their specific effects on proliferation, invasion and metastasis of colorectal carcinoma (CRC) are still poorly understood. The aim of the study is to investigate miR-155-5p effect on proliferation and invasion metastasis of CRC.


METHODS
Retrospectively analyzed clinicopathological parameters and fresh tissue samples of 372 colon cancer patients receiving radical surgery. HT-29 cells were transfected with mimics and inhibitors of miR-155-5p, respectively. Real-time reverse transcription-PCR was performed to measure miR-155-5p relative levels of tissues and cells.


RESULTS
miR-155-5p expression in cancer group was higher than that in normal group, with statistical differences (P<0.05). miR-155-5p expression was associated with tumor location, tumor grade, TNM staging and distant metastasis (P<0.05 for all parameters). Cell number of mimics group was higher than control group (P<0.01), and that of inhibitor group was lower than control group (P<0.05). Invasion and metastasis effect of mimics group were the highest and those of inhibitor group were the lowest.


CONCLUSIONS
miR-155-5p expression is up-regulated in most CRC and promotes proliferation, invasion and metastasis of CRC cells. It may play an essential role in tumorigenesis and tumor progression of CRC.
Department of Digestive Oncology, Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang, P.R. China.
Yan-Li
YL
Qu
Department of Abdominal Radiotherapy, Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang, P.R. China.
Hai-Feng
HF
Wang
Department of Gastrointestinal Surgery, Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang, P.R. China.
Zhen-Qiang
ZQ
Sun
Department of Digestive Oncology, Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang, P.R. China.
Yong
Y
Tang
Department of Digestive Oncology, Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang, P.R. China.
Xiao-Na
XN
Han
Department of Gynaecology, Cangzhou People's Hospital Cangzhou 061000, Hebei, P.R. China.
Xian-Bo
XB
Yu
Department of Gastrointestinal Surgery, Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, Xinjiang, P.R. China.
Kun
K
Liu
D000368:Aged; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary / Q000601:surgery; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
C524942:MIRN155 microRNA, human; D035683:MicroRNAs
HT-29 cell; colorectal carcinoma; invasion; miR-155-5p; proliferation; tumorigenesis