CXCL5 is associated with the increased risk of coronary artery disease.
OBJECTIVE
To investigate the relevant expression of CXCL5 and CXCR2 in human atherosclerotic coronary artery and to study the association between the CXCL5 variant and coronary artery disease (CAD) in a Chinese Han population.


METHODS
CXCL5 and CXCR2 expression in human coronary arteries was detected by immunohistochemical staining and western blotting analysis. The association between the CXCL5 variant and CAD was determined in a community-based sample by PCR-direct sequence analysis in a Chinese Han population. Finally, plasma CXCL5 levels were measured in a case-control study of CAD patients.


RESULTS
We found that CXCL5 and CXCR2 expressions were higher in atherosclerotic coronary arteries plaque than in the normal coronary arteries. CXCL5 and CXCR2 expression levels increased in line with coronary artery lesion stages. A functional nonsynonymous -156 G/C in the CXCL5 promoter region was associated with CAD and plasma CXCL5 levels were significantly increased in CAD patients compared with control subjects (3891.21±1403.08 vs. 2812.39±840.62 pg/ml, P<0.05). Individuals with the CXCL5 promoter -156 G/C variant C/C and G/C carriers had higher plasma CXCL5 levels than those with the G/G genotype carriers in both CAD patients and control participants.


CONCLUSIONS
CXCL5 and CXCR2 are enriched in human atherosclerotic coronary artery. Our findings show that the CXCL5 variant might be a genetic risk factor for the susceptibility of CAD and the CXCL5 promoter -156 G/C C allele might be an independent predictor for CAD. CXCL5 may be a useful molecular marker and a possible target for the treatment of CAD.
aDepartment of Cardiology, Shengjing Hospital of China Medical University, Shenyang bDepartment of Cardiology, Cardiovascular Research Institute, the General Hospital of Shenyang, Military, Shenyang, Liaoning, China.
Xiao-Zeng
XZ
Wang
Li-Wen
LW
Liu
Xiao-Mo
XM
Du
Ruo-Xi
RX
Gu
Zhi-Jun
ZJ
Sun
D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D015153:Blotting, Western; D016022:Case-Control Studies; D054365:Chemokine CXCL5 / Q000235:genetics* / Q000378:metabolism; D002681:China; D003324:Coronary Artery Disease / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000378:metabolism*; D023063:Receptors, Interleukin-8B / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D012333:RNA, Messenger; D023063:Receptors, Interleukin-8B