Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology.
This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively.
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.
Hugh W F
HW
Kingston
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Churchill Hospital, Headington, Oxford, United Kingdom.
Stuart D
SD
Blacksell
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Ampai
A
Tanganuchitcharnchai
Chiangrai Prachanukhru Hospital, Chiangrai, Thailand.
Achara
A
Laongnualpanich
Oxford University Clinical Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal.
Buddha
B
Basnyat
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Churchill Hospital, Headington, Oxford, United Kingdom.
Nicholas P J
NP
Day
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Churchill Hospital, Headington, Oxford, United Kingdom parigi@tropmedres.ac.
Daniel H
http://orcid.org/0000-0003-4743-5987
DH
Paris
D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D002846:Chromatography, Affinity / Q000592:standards*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D012285:Orientia tsutsugamushi / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012612:Scrub Typhus / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000592:standards*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic