Retinoblastoma-binding protein 2 induces epithelial-mesenchymal transition in esophageal squamous cancer cells.
OBJECTIVE
To investigate the effect of retinoblastoma-binding protein 2 (RBP2) on epithelial-mesenchymal transition (EMT) in esophageal squamous cancer cells and to compare the effect of RBP2 in lung squamous cancer cells and esophageal squamous cancer cells.


RESULTS
When transfected with RBP2 siRNA, the migrated cells were 36.3 ± 6.03 by transwell migration assay, compared to 107 ± 6.7 cells in the control group. The mRNA level of epithelial cadherin (E-cadherin) was 1.54 ± 0.14 times higher than in the control group, and that of neural cadherin (N-cadherin) fell to 0.76 ± 0.03 times. The relative luciferase activity of E-cadherin promoter rose to 3.84 ± 0.23 times. Correspondingly, the expression of E-cadherin protein increased and that of N-cadherin protein decreased. When SK-MES-1 cells were transfected with RBP2 siRNA, their relative mRNA level of E-cadherin was 8.6 ± 0.37 times as high as that in control group, which was higher than that in Eca-109 cells. The E-cadherin protein was also greater in SK-MES-1 cells.


CONCLUSIONS
RBP2 could induce EMT in esophageal cancer cells and exert a greater effect on the expression of E-cadherin in lung squamous cells than in esophageal squamous cells.
Department of Radiation Oncology, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, 250012, China. 807884225@qq.com.
Naian
N
Qiao
Department of Emergency, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, 250012, China. shikunwang2010@163.com.
Shikun
S
Wang
Department of Radiation Oncology, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, 250012, China. hulikuan_2013@163.com.
Likuan
http://orcid.org/0000-0003-1677-2091
L
Hu
D015820:Cadherins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D004938:Esophageal Neoplasms / Q000473:pathology*; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008954:Models, Biological; D056485:Retinoblastoma-Binding Protein 2 / Q000235:genetics / Q000378:metabolism*
D015820:Cadherins; D056485:Retinoblastoma-Binding Protein 2
Epithelial cadherin; Epithelial-mesenchymal transition; Esophageal squamous cancer; Lung squamous cancer; Neural cadherin; Retinoblastoma-binding protein 2