Calcium alginate enhances wound healing by up-regulating the ratio of collagen types I/III in diabetic rats.
Calcium alginate has been proved to favor the skin ulcer healing and collagen synthesis was a critical factor for the wound closure. The present study was to elucidate the mechanism of calcium alginate on the diabetes skin ulceration. Calcium alginate dressing was applied daily on the full-thickness exercising wound created on the back of diabetic rat model as Alg-group (n=6), and the vaseline dressing was used as control (n=6). Rats were respectively sacrificed and the wound tissues were removed and used for the evaluation of various biochemical analysis contained collagen (type I and III) by Western blotting and hydroxyproline level changes by ELISA assay at 3 d, 7 d and 14 d after wounding. The expression of skin collagen I in Alg-group was enhanced from day 3 (0.66 ± 0.25 vs. 0.42 ± 0.09, P<0.05) to day 14 (1.09 ± 0.14 vs. 0.78 ± 0.16, P<0.05). However, no significant difference of collagen III expression was found between two groups during wound healing (P>0.05). And the ratio of collagen I/III in Alg-group was greater than that of Vas-group at day 7 (1.07 ± 0.31 vs. 0.77 ± 0.11, P<0.05) and 14 (1.18 ± 0.30 vs. 0.83 ± 0.14, P<0.05). The hydroxyproline level in skin homogenate of Alg-group was higher than that of Vas-group from day 3 (30.29 ± 0.92 ng/ml vs. 27.52 ± 0.83 ng/ml, P<0.05) to day 14 (89.58 ± 4.97 ng/ml vs. 79.30 ± 4.42 ng/ml, P<0.05). Calcium alginate accelerates the process of wound healing through improving type I collagen synthesis and increasing ratio of collagen I/III in diabetic rats.
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Tao
T
Wang
Shanghai Qisheng Institute for Biological Material Research Shanghai, China.
Qisheng
Q
Gu
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Jun
J
Zhao
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Jiacai
J
Mei
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Mingzhe
M
Shao
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Ye
Y
Pan
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Jian
J
Zhang
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Haisheng
H
Wu
Department of Vascular Surgery, Sixth People's Hospital of Shanghai, Shanghai Clinical Medical Center of Diabetes, Multidisciplinary Collaboration Center of Diabetic Foot 600 Yishan Road, Shanghai, China.
Zhen
Z
Zhang
Department of Endocrinology and Metabolism, Shanghai Key Laboratory of Diabetes, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai Clinical Medical Center of Diabetes, Shanghai Key Clinical Center of Metabolic Diseases, Multidisciplinary Collaboration Group of Diabetic Foot, Shanghai Institute for Diabetes Shanghai, China.
Fang
F
Liu
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004305:Dose-Response Relationship, Drug; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012883:Skin Ulcer / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D000464:Alginates; D024042:Collagen Type I; D024061:Collagen Type III; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D006909:Hydroxyproline
Calcium alginate; collagen type I/III; diabetic wound