Changes in Genetic Diversity from Field to Laboratory During Colonization of Anopheles darlingi Root (Diptera: Culicidae).
The process of colonizing any arthropod species, including vector mosquitoes, necessarily involves adaptation to laboratory conditions. The adaptation and evolution of colonized mosquito populations needs consideration when such colonies are used as representative models for pathogen transmission dynamics. A recently established colony of Anopheles darlingi, the primary malaria vector in Amazonian South America, was tested for genetic diversity and bottleneck after 21 generations, using microsatellites. As expected, laboratory An. darlingi had fewer private and rare alleles (frequency < 0.05), decreased observed heterozygosity, and more common alleles (frequency > 0.50), but no significant evidence of a bottleneck, decrease in total alleles, or increase in inbreeding compared with field specimens (founder population). Low-moderate differentiation between field and laboratory populations was detected. With these findings, and the documented inherent differences between laboratory and field populations, results of pathogen transmission studies using this An. darlingi colony need to be interpreted cautiously.
Department of Biomedical Sciences, School of Public Health, University at Albany (State University of New York), Albany, New York; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Medicine, Division of Infectious Diseases, University of California San Diego, La Jolla, California; Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Ciencias Celulares y Moleculares, Laboratorio de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru wlainhart@albany.edu.
William
W
Lainhart
Department of Biomedical Sciences, School of Public Health, University at Albany (State University of New York), Albany, New York; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Medicine, Division of Infectious Diseases, University of California San Diego, La Jolla, California; Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Ciencias Celulares y Moleculares, Laboratorio de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru.
Sara A
SA
Bickersmith
Department of Biomedical Sciences, School of Public Health, University at Albany (State University of New York), Albany, New York; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Medicine, Division of Infectious Diseases, University of California San Diego, La Jolla, California; Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Ciencias Celulares y Moleculares, Laboratorio de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru.
Marta
M
Moreno
Department of Biomedical Sciences, School of Public Health, University at Albany (State University of New York), Albany, New York; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Medicine, Division of Infectious Diseases, University of California San Diego, La Jolla, California; Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Ciencias Celulares y Moleculares, Laboratorio de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru.
Carlos Tong
CT
Rios
Department of Biomedical Sciences, School of Public Health, University at Albany (State University of New York), Albany, New York; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Medicine, Division of Infectious Diseases, University of California San Diego, La Jolla, California; Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Ciencias Celulares y Moleculares, Laboratorio de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru.
Joseph M
JM
Vinetz
Department of Biomedical Sciences, School of Public Health, University at Albany (State University of New York), Albany, New York; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Medicine, Division of Infectious Diseases, University of California San Diego, La Jolla, California; Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Ciencias Celulares y Moleculares, Laboratorio de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru.
Jan E
JE
Conn
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D014644:Genetic Variation; D007178:Inbreeding; D007303:Insect Vectors / Q000235:genetics; D007753:Laboratories; D008288:Malaria / Q000635:transmission; D018895:Microsatellite Repeats / Q000235:genetics