Integrated in silico and experimental methods revealed that Arctigenin inhibited angiogenesis and HCT116 cell migration and invasion through regulating the H1F4A and Wnt/β-catenin pathway.
Arctigenin (ARG) has been previously reported to exert diverse biological activities including anti-proliferation, anti-inflammatory, and antiviral, etc. In the current study, the anti-metastasis and anti-angiogenesis activities of ARG were investigated. To further understand how ARG played these bioactivities, proteomic approaches were used to profile the proteome changes in response to ARG treatment using 2DE-MS/MS. Using these approaches, a total of 50 differentially expressed proteins were identified and clustered. Bioinformatics analysis suggested that multiple signalling pathways were involved. Moreover, ARG induced anti-metastatic and anti-angiogenesis activities were mainly accompanied by a deactivation of the Wnt/β-catenin pathway in HCT116 cells.
Department of Pharmacy, Hospital of the University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu, 610072, P. R. China. lzxlulu@126.com.
Shouyue
S
Zhang
Jie
J
Li
Sicheng
S
Song
Jing
J
Li
Rongsheng
R
Tong
Zhihe
Z
Zang
Qinglin
Q
Jiang
Lulu
L
Cai
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019295:Computational Biology; D003198:Computer Simulation; D005663:Furans / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D045325:HCT116 Cells; D006657:Histones / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D060066:Protein Interaction Maps / Q000187:drug effects; D040901:Proteomics; D060449:Wnt Signaling Pathway / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects; D015027:Zebrafish / Q000235:genetics
D005663:Furans; D006657:Histones; D017705:Lignans; C071942:arctigenin