Does Solution Viscosity Scale the Rate of Aggregation of Folded Proteins?
Viscosity effects on the kinetics of complex solution processes have proven hard to predict. To test the viscosity effects on protein aggregation, we use the crystallization of the protein glucose isomerase (gluci) as a model and employ scanning confocal and atomic force microscopies at molecular resolution, dynamic and static light scattering, and rheometry. We add glycerol to vary solvent viscosity and demonstrate that glycerol effects on the activation barrier for attachment of molecules to the crystal growth sites are minimal. We separate the effects of glycerol on crystallization thermodynamics from those on the rate constant for molecular attachment. We establish that the rate constant is proportional to the reciprocal viscosity and to the protein diffusivity. This finding refutes the prevailing crystal growth paradigm and illustrates the application of fundamental kinetics laws to solution crystallization.
†Structural Biology Brussels, Flanders Institute for Biotechnology (VIB), Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Mike
M
Sleutel
‡Laboratorio de Estudios Crystalograficos, IACT, CSIC-U.Granada, P.T. Ciencias de la Salud, Avenida del conocimiento s/n, 18100 Armilla (Granada), Spain.
Alexander E S
AE
Van Driessche
Weichun
W
Pan
⊥Centre for Surface Chemistry and Catalysis, Department of Microbial and Molecular Systems, K.U. Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium.
Erwin K
EK
Reichel
†Structural Biology Brussels, Flanders Institute for Biotechnology (VIB), Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Dominique
D
Maes
Peter G
PG
Vekilov
atomic force microscopy; crystallization mechanism; friction coefficient; glucose isomerase; rate laws; scanning confocal microscopy; step kinetic coefficient