Effects of novel auto-inducible medium on growth, activity and CO₂ capture capacity of Escherichia coli expressing carbonic anhydrase.
A glucose-based auto-inducible medium (glucose-AIM) has been developed to enhance both growth and expression of lac operon-linked carbonic anhydrase (CA) expression in a recombinant strain of Escherichia coli. When the E. coli expressing CA was grown on various media, the glucose-based auto-inducible medium (glucose AIM) resulted in a CA activity of 1022 mU OD(600 nm)(-1) mL(-1) at 24 h and a specific growth rate of 0.082 h(-1). The CA activity was four to fourteen times higher than those by LB-IPTG. The E. coli expressing CA grown on the glucose-AIM showed highest activity at pH8.5 while it kept high stability up to 40°C and an inlet CO2 concentration of 6%. These findings indicate that the glucose-AIM would be a cost-effective medium to support high cell growth, CA activity and stability for effective CO2 capture.
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Stuart K
SK
Watson
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA. Electronic address: ekan@hawaii.edu.
Eunsung
E
Kan
D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D005947:Glucose
Auto-inducible media; Carbon capture; Carbonic anhydrase; Enzyme characterization; Enzyme stability; Whole cell biocatalyst