Energy landscape investigation by wavelet transform analysis of atomic force spectroscopy data in a biorecognition experiment.
Force fluctuations recorded in an atomic force spectroscopy experiment, during the approach of a tip functionalized with biotin towards a substrate charged with avidin, have been analyzed by a wavelet transform. The observation of strong transient changes only when a specific biorecognition process between the partners takes place suggests a drastic modulation of the force fluctuations when biomolecules recognize each other. Such an analysis allows to investigate the peculiar features of a biorecognition process. These results are discussed in connection with the possible role of energy minima explored by biomolecules during the biorecognition process.
Biophysics and Nanoscience Centre, CNISM -DEB, Universita' della Tuscia, 01100, Viterbo, Italy. bizzarri@unitus.it.
Anna Rita
AR
Bizzarri
D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D013816:Thermodynamics; D058067:Wavelet Analysis
D001360:Avidin; D001710:Biotin
Atomic force spectroscopy; Biotin–avidin; PACS; Wavelet transform