Profile of cytokines in the lungs of BALB/c mice after intra-nasal infection with Histoplasma capsulatum mycelial propagules.
The host pulmonary response to the fungus Histoplasma capsulatum was evaluated, through the profile of cytokines detected by the MagPix magnetic beads platform in lung homogenates and by lung-granulomas formation, from mice intra-nasally infected with mycelial propagules (M-phase) of two virulent H. capsulatum strains, EH-46 and G-217B. Results highlight that mice lung inflammatory response depends on the H. capsulatum strain used, during the first step of the fungal infection. IL-1β and TNF-α increased their concentrations in mice infected with both strains. The highest levels of IL-6, IL-17, and IL-23 were found in EH-46-infected mice, whereas levels of IL-22 were variable at all post-infection times for both strains. Significant increases of IL-12, IFN-γ, IL-4, and IL-10 were associated to EH-46-infected mice. Histological lung findings from EH-46-infected mice revealed incipient and numerous well-developed granulomas, distributed in lung-lobes at the 14th and the 21st days after infection, according to cytokine profiles.
Laboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), México DF, Mexico; Unidad de Micología Médica y Experimental, Corporación para Investigaciones Biológicas, Medellín, Colombia.
Jorge Humberto
JH
Sahaza
Departamento Micología, INEI ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
Roberto
R
Suárez-Alvarez
Laboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), México DF, Mexico; Colección Nacional de Cultivos Microbianos, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México DF, Mexico.
Daniel Alfonso
DA
Estrada-Bárcenas
Laboratorio de Filogenia del Sistema Inmune de Piel y Mucosas, Departamento de Biología Celular y Tisular, Facultad de Medicina, UNAM, México DF, Mexico.
Armando
A
Pérez-Torres
Laboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), México DF, Mexico. Electronic address: emello@unam.mx.
Maria Lucia
ML
Taylor
D000818:Animals; D016207:Cytokines / Q000276:immunology* / Q000378:metabolism*; D006658:Histoplasma / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D006660:Histoplasmosis / Q000276:immunology* / Q000382:microbiology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000276:immunology* / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D025282:Mycelium / Q000276:immunology / Q000472:pathogenicity; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D000097043:Interleukin-22
D016207:Cytokines; D007371:Interferon-gamma; D018664:Interleukin-12; D015850:Interleukin-6; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22
Cytokines; H. capsulatum; Intra-nasal infection; Mycelial propagules