Gadd45b Mediates Electroconvulsive Shock Induced Proliferation of Hippocampal Neural Stem Cells.
BACKGROUND
Electroconvulsive shock (ECS), also known as an electroconvulsive therapy (ECT), is an effective and safe treatment for neuropsychiatric disorders including pharmacoresistant major depressive disorder. Previous research in animal models suggests ECS efficacy is achieved by Gadd45b-mediated increases in adult hippocampal neurogenesis.


OBJECTIVE
The present study aims to delineate the role of Gadd45b in mediating proliferation of neural stem cell types including quiescent radial glia-like (RGL) and amplifying non-radial glia-like (non-RGL) neural precursors following ECS.


METHODS
RGL and non-RGL neural stem cell populations defined by co-localization of MCM2+ and nestin+ cells and morphologically by the presence of radial processes were stereologically analyzed.


RESULTS
ECS increased hippocampal density of both quiescent RGLs and amplifying non-RGLs.


CONCLUSIONS
Gadd45b mediates the action of ECS-induced proliferation through activation of quiescent neural stem cells.
Department of Neurologic Surgery, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
Heechul
H
Jun
Department of Neurologic Surgery, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
Syed
S
Mohammed Qasim Hussaini
Department of Neurologic Surgery, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
Chang Hoon
CH
Cho
Department of Neurologic Surgery, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
John
J
Welby
Department of Neurologic Surgery, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.|Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
Mi-Hyeon
MH
Jang
D000818:Animals; D000943:Antigens, Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D004597:Electroshock / Q000379:methods*; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D000097642:GADD45 Proteins
D000943:Antigens, Differentiation; C490125:Gadd45b protein, mouse; D000097642:GADD45 Proteins
Adult neurogenesis; Electroconvulsive shock; Gadd45b; Hippocampus; Neural stem cells