KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation.
Transcription factor SOX4 has been implicated in skeletal myoblast differentiation through the regulation of Cald1 gene expression; however, the detailed molecular mechanism underlying this process is largely unknown. Here, we demonstrate that SOX4 acetylation at lysine 95 by KAT5 (also known as Tip60) is essential for Cald1 promoter activity at the onset of C2C12 myoblast differentiation. KAT5 chromodomain was found to facilitate SOX4 recruitment to the Cald1 promoter, which is involved in chromatin remodeling at the promoter. Chromatin occupancy analysis of SOX4, KAT5, and HDAC1 indicated that the expression of putative SOX4 target genes during C2C12 myoblast differentiation is specifically regulated by the molecular switching of the co-activator KAT5 and the co-repressor HDAC1 on SOX4 transcriptional activation.
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
S-M
SM
Jang
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
J-W
JW
Kim
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
C-H
CH
Kim
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
J-H
JH
An
Department of Dermatology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
A
A
Johnson
Department of Dermatology, University of Colorado Denver Anschutz Medical Campus, Aurora, CO 80045, USA.
P I
PI
Song
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
S
S
Rhee
Department of Life Science, Chung-Ang University, Seoul 156-756, Korea.
K-H
KH
Choi
D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly; D017930:Genes, Reporter; D057809:HEK293 Cells; D051548:Histone Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D056284:Histone Deacetylase 1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D000074443:Lysine Acetyltransferase 5; D051379:Mice; D008969:Molecular Sequence Data; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D055751:SOXC Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
D002148:Calmodulin-Binding Proteins; D055751:SOXC Transcription Factors; C489990:Sox4 protein, mouse; D015534:Trans-Activators; D008156:Luciferases; D051548:Histone Acetyltransferases; C523065:Kat5 protein, mouse; D000074443:Lysine Acetyltransferase 5; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1