The molecular systemic and local effects of intra-tendinous injection of Platelet Rich Plasma in tendinosis: preliminary results on a rat model with ELISA method.
OBJECTIVE
the aim of our study was thus to quantify the effect of Platelet Rich Plasma (PRP) injection on systemic and local growth factors and to identify molecular markers in a rat model of patellar and Achilles tendinosis treated with PRP.


METHODS
twenty two rats were used for the study. Two healthy rats were used as control (T-). We induced tendinosis (T+) in 20 rats (80 tendons by injecting under ultrasonography (US) guidance Collagenase 1® (day 0 = D0, patellar=40 and Achilles=40). At D3, these 20 rats with tendinosis were separated in treatment by either PRP (PRPT+, n=28), physiological serum (PST+, n=28, control) US-guided intratendinous injection, or without no PRP or PS (T+, n=24, control of natural evolution of tendinopathy). Follow-up at D7, D13, D18 and D25 using serum sample and local tendon removal with ELISA technics and comparison between the 3 groups were performed.


RESULTS
during biological follow up, comparison of all serum samples of PRPT+, PST+ and T+ groups showed no significant modification of their biological markers at D7, D13, D18 and D25 (p>0.22). Comparison of immunological sample tendon markers of PRPT+, PST+ and T+ groups also showed no significant modification of markers at D7, D13, D18 and D25 (p>0.16) considering each biological marker and also all subgroups confounded.


CONCLUSIONS
our study strongly suggests that a single intratendinous US-guided injection of PRP in Achilles and patellar T+ doesn't increase biological markers such as growth factors compared to a control group in mid-term and long-term follow-up.
Centre d'Imagerie Ostéo-articulaire, Clinique du Sport de Bordeaux-Mérignac, France ; Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Benjamin
B
Dallaudiere
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Liliane
L
Louedec
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Marie Paule Jacob
MP
Lenet
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Lionel
L
Pesquer
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Elvind
E
Blaise
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Anne
A
Perozziello
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Jean Baptiste
JB
Michel
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Maryse
M
Moinard
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Philippe
P
Meyer
Department of MSK Radiology Department, CHU Pellegrin, Bordeaux, France.
Jean Michel
JM
Serfaty
ELISA; PRP; platelet; rat; tendinosis