Efficient siRNA Delivery Using PEG-conjugated PAMAM Dendrimers Targeting Vascular Endothelial Growth Factor in a CoCl2-induced Neovascularization Model in Retinal Endothelial Cells.
BACKGROUND
Choroidal neovascularization (CNV), also known as subretinal neovascularization, causes serious damage to the central vision as it happens more commonly in macula. The most important factor involved in angiogenesis is vascular endothelial growth factor (VEGF). By an RNAi technique, VEGF gene knockdown can be used to treat CNV. PEG-conjugated poly (amidoamine) (PEGPAMAM) dendrimers as a new type of synthetic polymers are very promising to be gene delivery carriers.


METHODS
To investigate siRNA delivery efficacy of PEG-PAMAM dendrimers, we prepared dendriplexes of PEG-PAMAM dendrimers with a fluorescence-labelled siRNA (PEG-PAMAM/FAM siRNA) or VEGF siRNA (PEG-PAMAM/VEGF siRNA), and studied transfection and downregulation efficacy of the dendriplexes in a cobalt chloride (CoCl2)-induced neovascularization model in retinal vascular endothelial cells (RF/6A).


RESULTS
Our results demonstrate that PEG-PAMAM dendrimers had significantly higher transfection efficiency to FAM siRNA than a commercial transfection reagent PEI (1.4-fold, P<0.001) measured by flow cytometry. Compared to the PEI/VEGF siRNA polyplexes, the dendriplexes of the PEG-PAMAM/VEGF siRNA more significantly downregulated VEGF gene expression (P < 0.01) at both mRNA and protein expression level. A tube formation assay also proved that the PEG-PAMAM/VEGF siRNA dendriplexes more significantly inhibited vascular-like formation than PEI/VEGF siRNA did (P < 0.001) in RF/6A.


CONCLUSIONS
This study demonstrated that G5-PEG was more efficient than PEI in facilitating siRNA delivery, downregulating VEGF expression and inhibiting vascular-like formation on RF/6A.
Lu
L
Xu
Wenwen
W
Shen
Bin
B
Wang
Xinlei
X
Wang
George
G
Liu
Yong
Y
Tao
38 Xueyuan Road, Peking University Institute of Cardiovascular Sciences, Peking University Health Science Center, Beijing 100191, China. ronaqi@bjmu.edu.cn.
Rong
R
Qi
D000818:Animals; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology*; D050091:Dendrimers / Q000737:chemistry*; D004195:Disease Models, Animal; D008251:Macaca; D009389:Neovascularization, Pathologic / Q000139:chemically induced*; D011092:Polyethylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012171:Retinal Vessels / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects*
D050091:Dendrimers; C104700:PAMAM Starburst; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D003035:Cobalt; D011092:Polyethylene Glycols; C018021:cobaltous chloride