Evaluation of the effects of fulvestrant and micronized progesterone on the post-operative adhesion formation and ovarian reserve in rat model with immunohistochemical and biochemical analysis.
OBJECTIVE
To evaluate the effects of fulvestrant and micronized progesterone on post-operative adhesion formation and ovarian reserve in a rat uterine horn adhesion.


METHODS
In this prospective randomized controlled trial, 32 female Wistar albino rats were randomly divided into four groups including control group (Group 1), the control adhesion group (Group 2), 1 mg/kg daily intramuscular fulvestrant received group (Group 3) and 1 mg/kg daily oral micronized progesterone received group (Group 4). The extent and severity of adhesions were scored and samples were taken from adhesion areas to investigate the grades of adhesions according to the immunohistochemical scoring system. Ovarian reserves were measured with anti-Müllerian hormone (AMH) and histological ovarian follicles count.


RESULTS
The extent, severity and total adhesion scores were reduced in all treatment groups compared to control adhesion group (Group 2). Similarly, immunohistochemical adhesion scores were lower in the treatment groups. AMH and follicle count were significantly found lower in adhesion groups compared with control group. However, treatment groups were found to have higher ovarian reserve compared to control adhesion group (Group 2).


CONCLUSIONS
Fulvestrant and micronized progesterone were found to reduce post-operative adhesion formations and have decreased detrimental effects of adhesion formation on ovarian reserve.
a Department of Obstetrics and Gynecology , Mugla Sitki Kocman University, School of Medicine , Mugla , Turkey .
Gokalp
G
Oner
b Department of Obstetrics and Gynecology , Erzincan University, School of Medicine , Erzincan , Turkey .
Pasa
P
Ulug
c Department of Pathology , and.
Elif
E
Demirci
d Department of Obstetrics and Gynecology , Erzurum University, School of Medicine , Erzurum , Turkey.
Yakup
Y
Kumtepe
c Department of Pathology , and.
Cemal
C
Gündogdu
D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D000077267:Fulvestrant; D065851:Ovarian Reserve / Q000187:drug effects*; D011374:Progesterone / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control*; D014599:Uterus / Q000187:drug effects / Q000601:surgery*
D000077267:Fulvestrant; D011374:Progesterone; D004958:Estradiol
Adhesion; fulvestrant; micronized progesterone; ovarian reserve; rat