The involvement of survivin in insulin-like growth factor 1-induced epithelial-mesenchymal transition in gastric cancer.
It has been identified that insulin-like growth factor 1 (IGF-1) activated various pathways of the epithelial-mesenchymal transition (EMT) in a couple of tumors. At the same time, survivin is implicated in EMT of gastric cancer (GC). To date, the impact of survivin on IGF-1-mediated EMT of GC has not been featured. In this work, we used the immunohistochemistry and molecular and cellular experiments to investigate the existence and significance of IGF-1 and survivin. Our findings revealed that survivin protein can be observed in majority of samples in all GC samples. Importantly, survivin expression has an obvious association with GC stage, and metastasis. In vitro, GC cell line BGC823 was treated with different concentrations of IGF-1, resulting in the activation of p-ERK, p-AKT, survivin, and the expression of EMT biomarkers, including N-cadherin, MMP2, and Snail. However, the silencing of survivin eradicated the expression IGF-1-induced EMT biomarkers and affected the migration and invasion of BGC823 cells. In conclusion, IGF-1 signaling activated survivin expression and controlled the expression of EMT biomarkers in the development of GC. This study lays a new stage for the molecular therapy of GC patients in the clinical treatment.
Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38#, Wuyingshan Road, Jinan, Shandong, China.
Chengjun
C
Li
Department of General Surgery, Guanzhuang Hospital of Anqiu City, Weifang, Shandong, China.
Jinbao
J
Li
Department of General Surgery, Huimin County Hospital of Shandong Province, Binzhou, Shandong, China.
Dawei
D
Wu
Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38#, Wuyingshan Road, Jinan, Shandong, China. hangangsdma@163.com.
Gang
G
Han
D000328:Adult; D000368:Aged; D015415:Biomarkers; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000077022:Survivin
C107489:BIRC5 protein, human; D015415:Biomarkers; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D007334:Insulin-Like Growth Factor I
EMT; GC; IGF-1; Survivin