Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat.
Measurements of glomerular filtration rate (GFR), and the fractional excretion of sodium (Na) and potassium (K) are critical in assessing renal function in health and disease. GFR is measured as the steady state renal clearance of inulin which is filtered at the glomerulus, but not secreted or reabsorbed along the nephron. The fractional excretion of Na and K can be determined from the concentration of Na and K in plasma and urine. The renal clearance of inulin can be demonstrated in an anesthetized animal which has catheters in the femoral artery, femoral vein and bladder. The equipment and supplies used for this procedure are those commonly available in a research core facility, and thus makes this procedure a practical means for measuring renal function. The purpose of this video is to demonstrate the procedures required to perform a lab demonstration in which renal function is assessed before and after a diuretic drug. The presented technique can be utilized to assess renal function in rat models of renal disease.
Tactical Combat Casualty Care Research, U.S. Army Institute of Surgical Research.
Carmen
C
Hinojosa-Laborde
Department of Pharmacology and Toxicology, Michigan State University; jespers1@msu.edu.
Brian
B
Jespersen
Southwest National Primate Research Center, Texas Biomedical Research Institute.
Robert
R
Shade
D000818:Animals; D005919:Glomerular Filtration Rate / Q000502:physiology*; D007444:Inulin / Q000494:pharmacology; D007674:Kidney Diseases / Q000175:diagnosis / Q000503:physiopathology / Q000652:urine; D007677:Kidney Function Tests / Q000379:methods*; D007678:Kidney Glomerulus / Q000502:physiology; D009399:Nephrons / Q000502:physiology; D011188:Potassium / Q000652:urine; D051381:Rats; D012964:Sodium / Q000652:urine
D007444:Inulin; D012964:Sodium; D011188:Potassium