Investigation on the proteome response of transplanted blue mussel (Mytilus sp.) during a long term exposure experiment at differently impacted field stations in the German Bight (North Sea).
In a pilot field study the proteome response of Mytilus sp. was analyzed in relation to the concentration of different trace metal contaminants. Over a period of eight month test organisms have been exposed at a near-shore station in the anthropogenic impacted estuary of the river Elbe and at an off-shore station in the vicinity of the Island of Helgoland in the German Bight (North Sea). The stations differ in their hydrological as well as chemical characteristics. The physiological biomarkers, such as condition index which have been continuously monitored during the experiment clearly indicate the effects of the different environmental conditions. Multiple protein abundance changes were detected utilizing the techniques of two dimensional gel electrophoresis (2dGE) and consequently proteins arising as potential candidates for ecotoxicological monitoring have been identified by MALDI-ToF and ToF/ToF mass spectrometry. Different cytoskeletal proteins, enzymes of energy metabolism, stress proteins and one protein relevant for metal detoxification have been pointed out.
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Coastal Research, Department Marine Bioanalytical Chemistry, Max-Planck St. 1, D-21502 Geesthacht, Germany. Electronic address: heike.helmholz@hzg.de.
Heike
H
Helmholz
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Coastal Research, Department Marine Bioanalytical Chemistry, Max-Planck St. 1, D-21502 Geesthacht, Germany.
Stephan
S
Lassen
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Coastal Research, Department Marine Bioanalytical Chemistry, Max-Planck St. 1, D-21502 Geesthacht, Germany.
Christiane
C
Ruhnau
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Coastal Research, Department Marine Bioanalytical Chemistry, Max-Planck St. 1, D-21502 Geesthacht, Germany.
Daniel
D
Pröfrock
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Coastal Research, Department Marine Bioanalytical Chemistry, Max-Planck St. 1, D-21502 Geesthacht, Germany.
Hans-Burkhard
HB
Erbslöh
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Coastal Research, Department Marine Bioanalytical Chemistry, Max-Planck St. 1, D-21502 Geesthacht, Germany.
Andreas
A
Prange
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004784:Environmental Monitoring / Q000379:methods*; D005858:Germany; D049880:Mytilus edulis / Q000187:drug effects*; D009659:North Sea; D018384:Oxidative Stress / Q000187:drug effects*; D020543:Proteome; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D020543:Proteome; D014874:Water Pollutants, Chemical
Bioresponse; Contaminants; Electrophoresis; Mass spectrometry; Mytilus; North Sea; Proteomics