Afrostyrax lepidophyllus extracts exhibit in vitro free radical scavenging, antioxidant potential and protective properties against liver enzymes ion mediated oxidative damage.
BACKGROUND
Several studies described the phytochemical constituents of plants in relation with the free radical scavenging property and inhibition of lipid peroxidation. This study investigated the in vitro antioxidant property, and the protective effects of ethanolic and aqueous ethanol extract of the leaves and barks of Afrostyrax lepidophyllus (Huaceae) against ion mediated oxidative damages.


METHODS
Four extracts (ethanol and aqueous-ethanol) from the leaves and barks of A. lepidophyllus were used in this study. The total phenols content, the antiradical and antioxidant properties were determined using standard colorimetric methods.


RESULTS
The plant extracts had a significant scavenging potential on the 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitrite oxide (NO) and 2,2-azinobis (3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals with the IC50 varied between 47 and 200 µg/mL depending on the part of plant and the type of extract. The ethanol extract of A. lepidophyllus bark (GEE) showed the highest polyphenolic (35.33 ± 0.29) and flavonoid (12.00 ± 0.14) content. All the tested extracts demonstrated a high protective potential with the increased of superoxide dismutase, catalase and peroxidase activities.


CONCLUSIONS
Afrostyrax lepidophyllus extracts exhibited higher antioxidant potential and significant protective potential on liver enzymes.
Laboratory of Biochemistry, Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon. mouket2006@yahoo.fr.
Bruno Moukette
BM
Moukette
Laboratory of Biochemistry, Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon. apieme@yahoo.fr.
Constant Anatole
CA
Pieme
Department of Biochemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon. brbiapa@yahoo.fr.
Prosper Cabral Nya
PC
Biapa
Laboratory of Biochemistry, Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon. movicky@yahoo.fr.
Vicky Jocelyne Ama
VJ
Moor
Laboratory of Biochemistry, Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon. eusbong@yahoo.com.
Eustace
E
Berinyuy
Laboratory of Biochemistry, Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon. jngogang@yahoo.fr.
Jeanne Yonkeu
JY
Ngogang
D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds; D002374:Catalase / Q000096:biosynthesis / Q000378:metabolism; D003124:Colorimetry; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D016166:Free Radical Scavengers; D005609:Free Radicals; D017665:Hydroxyl Radical / Q000737:chemistry; D020128:Inhibitory Concentration 50; D007477:Ions; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008982:Molybdenum / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D009195:Peroxidase / Q000096:biosynthesis; D019800:Phenol / Q000737:chemistry; D010851:Picrates; D024301:Plant Bark / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry*; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar; D013451:Sulfonic Acids / Q000737:chemistry; D013482:Superoxide Dismutase / Q000096:biosynthesis; D014197:Trees
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D005609:Free Radicals; D007477:Ions; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D019800:Phenol; D000431:Ethanol; D008982:Molybdenum; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D010100:Oxygen