The difference between blood-associated and water-associated herbs of Danggui-Shaoyao San in theory of TCM, based on serum pharmacochemistry.
Danggui-Shaoyao San (DSS) is a famous Chinese formula for activating blood circulation and promoting urination. This study was to investigate the difference of material basis between a blood-associated herbs group and a water-associated herbs group. According to the theory of traditional Chinese medicine, the formula can be divided into a blood-associated herbs group (Angelica sinensis, Paeonia lactiflora and Ligusticum chuanxiong) and a water-associated herbs group (Atractylodes macrocephala, Alisma orientale and Poria cocos). The HPLC fingerprint of the formula was established for quality control. Serum samples from rats, orally administrated DSS, and the decomposed recipes of DSS, were analyzed by HPLC-DAD and the transitional blood components of DSS were identified. Twenty-one common peaks were identified in the fingerprint of DSS. Contents of paeoniflorin, albiflorin, ferulic acid and alisol B 23-acetate in co-decoction were significantly higher than those in individual decoction. Eleven peaks belonged to the blood-associated herbs group (four metabolites and seven prototype components; paeoniflorin and ferulic acid appeared in prototype components), whereas six peaks belonged to the water-associated herbs group (three metabolites and three prototype components). It was concluded that the serum pharmacochemistry is a meaningful approach for clarifying the difference between blood-associated and water-associated herbs in chemical composition.
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Yunlai
Y
Wang
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Guoqiang
G
Li
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Yu
Y
Zhou
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Dengke
D
Yin
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Chunlei
C
Tao
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Lan
L
Han
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Xiaoli
X
Yue
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Yongfu
Y
Pan
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Yao
Y
Yao
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Daiyin
D
Peng
Anhui University of Chinese Medicine, Meishan Road 103, Hefei, 230038, China.
Fan
F
Xu
D000818:Animals; D001952:Bridged-Ring Compounds / Q000032:analysis; D002783:Cholestenones / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D005960:Glucosides / Q000032:analysis; D008297:Male; D039821:Monoterpenes / Q000032:analysis; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry; D006027:Glycosides
D001952:Bridged-Ring Compounds; D002783:Cholestenones; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D014867:Water; C526957:alisol B 23-acetate; C531150:danggui-shaoyao-san; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C004999:ferulic acid
Danggui-Shaoyao San; chromatographic fingerprints; compatility laws; quality control; serum pharmacochemistry