Biglycan enhances the ability of migration and invasion in endometrial cancer.
OBJECTIVE
This study aimed to confirm that biglycan (BGN) can promote the migration and invasion in endometrial cancer both in vitro and in vivo and the possible therapeutic value of BGN in endometrial cancer.


METHODS
Western blot was used to screen out the higher protein level of BGN in human endometrial cancer cells; BGN knocked down cells were constructed by lentiviral transfection; The effect of BGN in endometrial cancer detected by wound healing, transwell migration, and invasion, endothelial tube formation assay in vitro, and xenograft model in vivo.


RESULTS
(1) We found that BGN expression level is higher in the Ishikawa (ISK, high differentiation) and AN3CA (poor differentiation) cells than other endometrial cancer cells. (2) BGN enhances endometrial cancer cell wound healing, invasion, and migration ability and formation ability of endothelial cells in vitro. Xenograft model has confirmed the outcome in vivo.


CONCLUSIONS
BGN might play an important role on metastasis in human endometrial cancer and it might be a target marker for the molecular therapy of advanced and recurrence endometrial cancer.
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Hengzi
H
Sun
Jinan Maternity and Child Care Hospital, Jinan, Shandong, China.
Xiang
X
Wang
Department of Obstetrics and Gynecology, Affiliated Hospital of Taishan Medical University, Taian, Shandong, China.
Yifang
Y
Zhang
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Xiaoxia
X
Che
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Zhiming
Z
Liu
Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
Lili
L
Zhang
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Chunping
C
Qiu
Department of Traditional Chinese Medicine, Shandong University, Jinan, China.
Qingtao
Q
Lv
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, 250012, Shandong, China. qljiangjie@sdu.edu.cn.
Jie
J
Jiang
D058578:Biglycan / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D042783:Endothelial Cells / Q000473:pathology / Q000502:physiology; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D014945:Wound Healing / Q000235:genetics
D058578:Biglycan
Biglycan; Endometrial cancer; Invasion; Metastasis