Benzo-α-pyrene induced oxidative stress in Caenorhabditis elegans and the potential involvements of microRNA.
In the present study oxidative stress induced by Benzo-α-pyrene (BaP) exposure and the potential involvements of microRNA were investigated. The Caenorhabditis elegans (C. elegans) was applied as model organism. The C. elegans at L1-stage were randomly divided into 4 groups and exposed to 0, 0.2, 2.0, and 20μM BaP for 30h. Expressions of SKiNhead-1 (SKN-1), gamma-glutamine cysteine synthase heavy chain (GCS-1), and their potential regulatory factors in insulin/IGF-1/FOXO signaling pathway and the p38 MAPK pathway were analyzed. The expressions of potentially involved microRNAs were investigated as well. Results demonstrated that expressions of SKN-1 and GCS-1 were altered significantly following BaP exposure (P<0.05). Meanwhile, expressions of multiple related factors were changed after BaP treatments. The altered factors include AKT-1, DAF-16, glutathione synthetase (GSS-1), glutathione S-transferase-24 (GST-24), mitogen-activated protein kinase kinase-4 (MKK-4), multidrug resistance-associated protein-1 (MRP-1), and pyruvate dehydrogenase kinase-2 (PDHK-2) (P<0.05). In addition, results showed that exposure to BaP led to altered expressions of microRNA. Out of the 28 tested microRNAs, expressions of miR-1, miR-355, miR-50, miR-51, miR-58, miR-796, miR-797, and miR-84 were modified. Findings of the present study include that BaP exposure caused oxidative stress in C. elegans. The expressional response of GCS-1 to BaP exposure might be independent of the regulation of SKN-1 in C. elegans. The microRNAs might be involved in the regulations of SKN-1 and GCS-1 expression following BaP exposure in C. elegans.
Department of Preventive Medicine, Wenzhou Medical University, Wenzhou 325035, PR China; Department of Biology, East Carolina University, Greenville, NC 27858, USA. Electronic address: hongmeiwwu@hotmail.com.
Hongmei
H
Wu
Department of Preventive Medicine, Wenzhou Medical University, Wenzhou 325035, PR China.
Chenping
C
Huang
Department of Biology, East Carolina University, Greenville, NC 27858, USA.
Faten A
FA
Taki
Department of Biology, East Carolina University, Greenville, NC 27858, USA.
Yanqiong
Y
Zhang
Department of Biology, East Carolina University, Greenville, NC 27858, USA.
Dorothy L
DL
Dobbins
Department of Preventive Medicine, Wenzhou Medical University, Wenzhou 325035, PR China.
Lin
L
Li
Department of Preventive Medicine, Wenzhou Medical University, Wenzhou 325035, PR China.
Hongtao
H
Yan
Department of Biology, East Carolina University, Greenville, NC 27858, USA. Electronic address: panx@ecu.edu.
Xiaoping
X
Pan
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
D001564:Benzo(a)pyrene; D029742:Caenorhabditis elegans Proteins; D004785:Environmental Pollutants; D005982:Glutathione Transferase; D007334:Insulin-Like Growth Factor I; D035683:MicroRNAs; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D014157:Transcription Factors; D048051:p38 Mitogen-Activated Protein Kinases; C421109:multidrug resistance-associated protein 1
Benzo-α-pyrene; Caenorhabditis elegans; Gamma-glutamine cysteine synthase heavy chain (GCS-1); Oxidative stress; SKiNhead-1 (SKN-1); microRNA