The potential of plasma miRNAs for diagnosis and risk estimation of colorectal cancer.
Circulating microRNAs (miRNAs) were recognized to be potential non-invasive biomarkers for colorectal cancer (CRC) detection and prediction. Meanwhile, the association of the expression of plasma miRNAs with the risk of CRC patients has rarely been analyzed. Therefore, we conducted this study to evaluate the value of plasma miRNAs for CRC diagnosis and risk estimation. Fasting blood samples from 100 CRC patients and 79 cancer-free controls were collected. Plasma miR-106a, miR-20a, miR-27b, miR-92a and miR-29a levels were detected by RT-qPCR. Sensitivity and specificity were employed to evaluate the diagnostic value of miRNAs for CRC. Univariate and multivariate logistic regression were employed to analyze the association between miRNAs expression and CRC risk. As results, miR-106a and miR-20a were elevated in the patients with CRC. The sensitivity of miR-106a was 74.00% and the specificity was 44.40%, while the cutoff value was 2.03. As for miR-20a, the sensitivity was 46.00% and specificity was 73.42% when employed 2.44 as cutoff value. High expression of plasma miR-106a increased CRC risk by 1.80 -fold. Plasma miR-106a and miR-20a may as noninvasive biomarkers for detecting the CRC. High expression of miR-106a associated with CRC risk.
Department of Epidemiology, Public Health College, Harbin Medical University Harbin, Heilongjiang 150081, P. R. China.
Wang-Yang
WY
Chen
Department of Epidemiology, Public Health College, Harbin Medical University Harbin, Heilongjiang 150081, P. R. China.
Xiao-Juan
XJ
Zhao
Department of Epidemiology, Public Health College, Harbin Medical University Harbin, Heilongjiang 150081, P. R. China.
Zhi-Fu
ZF
Yu
Department of Epidemiology, Public Health College, Harbin Medical University Harbin, Heilongjiang 150081, P. R. China.
Fu-Lan
FL
Hu
Department of Epidemiology, Public Health College, Harbin Medical University Harbin, Heilongjiang 150081, P. R. China.
Yu-Peng
YP
Liu
Department of Abdominal Surgery, The Tumor Hospital of Harbin Medical University Harbin, Heilongjiang Province, P. R. China.
Bin-Bin
BB
Cui
Department of Tumor Surgery, The Forth Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang Province, P. R. China.
Xin-Shu
XS
Dong
Department of Epidemiology, Public Health College, Harbin Medical University Harbin, Heilongjiang 150081, P. R. China.
Ya-Shuang
YS
Zhao
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D016015:Logistic Models; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D016017:Odds Ratio; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors
D014408:Biomarkers, Tumor; D035683:MicroRNAs
colorectal cancer; diagnosis; microRNA; plasma; risk factor