Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides.
Polysaccharide extraction from Dipsacus asperoides roots (DAP) was proved to possess strong antioxidant activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities, inhibiting β-carotene bleaching and strong reducing power. Cell assay demonstrated that the crude DAP possessed antioxidant activity and were effective against H2O2-induced L02 cells injury. Then, response surface methodology (RSM) was applied to optimize the ultrasonic extraction of DAP. The optimum variables given by central composite design (CCD) were as follows: ratio of water to raw material, 38.61mL/g; ultrasonic power, 308.68W; extraction time, 38.61min; and extraction temperature, 89°C. Under these conditions, the maximum yield of DAP obtained was 7.12±0.45%. Moreover, high performance liquid chromatography (HPLC) analysis suggested that the monosaccharide compositions of DAP contained primarily mannose, ribose, glucose, galactose, xylose and arabinose, with a molar ratio of 0.22:0.48:2.29:0.34:1.39:1.41. The results of the present study showed that DAP could be considered as potential sources of natural antioxidants.
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China.
Li-Hong
LH
Tan
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China.
Dan
D
Zhang
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China.
Bao
B
Yu
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China.
Sheng-Ping
SP
Zhao
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China.
Jian-Wei
JW
Wang
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China.
Ling
L
Yao
College of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China; The Lab of Traditional Chinese Medicine, Chongqing Medical University, 400016 Chongqing, PR China. Electronic address: cwgzd2001@hotmail.com.
Wei-Guo
WG
Cao
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D049917:Dipsacaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D019207:beta Carotene / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides; D019207:beta Carotene
Antioxidant activities; Dipsacus asperoides; Extraction optimization; Polysaccharide