Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus.
This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30-70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg(-1), with a maximum working temperature of 60 °C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices.
Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.
Fernanda
F
Croisfelt
Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.
Bianca C
BC
Martins
Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.
Robson
R
Rescolino
Diego F
DF
Coelho
School of Chemical Engineering, Unicamp, Campinas, SP, Brazil.
Beatriz
B
Zanchetta
Faculty of Pharmaceutical Sciences, Unicamp, Campinas, SP, Brazil.
Priscila G
PG
Mazzola
Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.
Luis Ricardo
LR
Goulart
School of Pharmaceutical Sciences, USP, São Paulo, SP, Brazil.
Adalberto
A
Pessoa
School of Chemical Engineering, Unicamp, Campinas, SP, Brazil.
Elias B
EB
Tambourgi
Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.
Edgar
E
Silveira
D020106:Acrylamide; D000180:Acrylic Resins; D031230:Ananas / Q000737:chemistry*; D001963:Bromelains / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D003692:Delayed-Action Preparations; D020100:Hydrogels / Q000737:chemistry
D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D020100:Hydrogels; D020106:Acrylamide; C052970:poly-N-isopropylacrylamide; D001963:Bromelains