Effect of Fluoride-Modified Titanium Surface on Early Adhesion of Irradiated Osteoblasts.
OBJECTIVE
The present study aimed to investigate the effect of fluoride-modified titanium surface on adhesion of irradiated osteoblasts.


METHODS
Fluoride-modified surface was obtained and the morphology, roughness, and chemical composition of the surface were evaluated by scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy, respectively. The adhesion of irradiated osteoblast-like cells, in terms of number, area, and fluorescence intensity on the titanium surface, was evaluated using immunofluorescence staining.


RESULTS
Numerous nanosize pits were seen only in the F-TiO surface. The pits were more remarkable and uniform on F-TiO surface than on TiO surface; however, the amplitude of peaks and bottoms on F-TiO surface appeared to be smaller than on TiO surface. The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface. The concentrations of main elements such as titanium, oxygen, and carbon were similar on both surfaces. The number of irradiated osteoblasts adhered on the control surface was larger than on fluoride-modified surface. Meanwhile, the cells on the fluoride-modified surface formed more actin filaments.


CONCLUSIONS
The fluoride-modified titanium surface alters the adhesion of irradiated osteoblasts. Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface.
Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
Jun Yuan
JY
Li
Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
Li Wu
LW
Zheng
Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
Li
L
Ma
Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Dora Lai Wan
DL
Kwong
Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
Lim Kwong
LK
Cheung
Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
Edmond Ho Nang
EH
Pow
D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000528:radiation effects; D001672:Biocompatible Materials / Q000008:administration & dosage*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D005459:Fluorides / Q000008:administration & dosage*; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects* / Q000528:radiation effects; D011878:Radiotherapy; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage*; D014965:X-Rays
D001672:Biocompatible Materials; D014025:Titanium; D005459:Fluorides; C032240:titanium tetrafluoride