Urease inhibitory profile of extracts and chemical constituents of Pistacia atlantica ssp. cabulica Stocks.
The current study was designed to evaluate the urease inhibitory profile of extract and fractions of Pistacia atlantica ssp. cabulica Stocks followed by bioactivity-guided isolated compounds. The crude extract was found significantly active with urease inhibitor (95.40% at 0.2 mg/mL) with IC50 values of 32.0 ± 0.28 μg/mL. Upon fractionation, ethyl acetate fraction displayed 100% urease inhibition with IC50 values of 19.9 ± 0.51 μg/mL at 0.2 mg/mL. However, n-hexane and chloroform fractions exhibited insignificant urease inhibition. Similarly, the isolated compound, transilitin (1) and dihydro luteolin (2) demonstrated marked urease attenuation with 95 and 98% respectively, at 0.15 mg/mL. Both the isolated compounds showed marked potency with IC50 values of 8.54 ± 0.54 and 9.58 ± 2.22 μg/mL, respectively. In short, both the extract and fractions and isolated compounds showed marked urease inhibition and thus a useful natural source of urease inhibition.
a Institute of Chemical Sciences , Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar , Peshawar , Pakistan.
Ghias
G
Uddin
a Institute of Chemical Sciences , Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar , Peshawar , Pakistan.
Ismail
a Institute of Chemical Sciences , Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar , Peshawar , Pakistan.|e Department of Geology , University of Swabi , Anbar -23561 , Khyber Pakhtunkhwa , Pakistan.
Abdur
A
Rauf
a Institute of Chemical Sciences , Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar , Peshawar , Pakistan.
Muslim
M
Raza
b Department of Pharmacy , Abdul Wali Khan University Mardan 23200 , Mardan.
Haroon
H
Khan
a Institute of Chemical Sciences , Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar , Peshawar , Pakistan.
Nasruddin
c H.E.J. Research Institute of Chemistry , International Center for Chemical and Biological Sciences, University of Karachi , Karachi -75270 , Pakistan.
Majid
M
Khan
d Department of Chemistry , COMSATS Institute of Information Technology , Abbottabad -22060 , Pakistan.
Umar
U
Farooq
d Department of Chemistry , COMSATS Institute of Information Technology , Abbottabad -22060 , Pakistan.
Ajmal
A
Khan
a Institute of Chemical Sciences , Centre for Phytomedicine and Medicinal Organic Chemistry, University of Peshawar , Peshawar , Pakistan.
Arifullah
D002725:Chloroform / Q000737:chemistry; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006586:Hexanes / Q000737:chemistry; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D027927:Pistacia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014510:Urease / Q000037:antagonists & inhibitors*
D003374:Coumarins; D004791:Enzyme Inhibitors; D006586:Hexanes; D010936:Plant Extracts; C000619096:transilitin; C026385:n-hexane; D002725:Chloroform; D014510:Urease
Pistacia atlantica ssp. cabulica Stocks; chemical constituents; urease inhibitory activity