title: Rapid Legionella pneumophila determination based on a disposable core-shell Fe₃O₄@poly(dopamine) magnetic nanoparticles immunoplatform.
abstract: A novel amperometric magnetoimmunoassay, based on the use of core-shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of Legionella pneumophila SG1. A specific capture antibody (Ab) was linked to the poly(dopamine)-modified magnetic nanoparticles (MNPs@pDA-Ab) and incubated with bacteria. The captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (Ab-HRP), and the resulting MNPs@pDA-Ab-Legionella neumophila-Ab-HRP were captured by a magnetic field on the electrode surface. The amperometric response measured at -0.15 V vs. Ag pseudo-reference electrode of the SPCE after the addition of H2O2 in the presence of hydroquinone (HQ) was used as transduction signal. The achieved limit of detection, without pre-concentration or pre-enrichment steps, was 10(4) Colony Forming Units (CFUs) mL(-1). The method showed a good selectivity and the MNPs@pDA-Ab exhibited a good stability during 30 days. The possibility of detecting L. pneumophila at 10 CFU mL(-1) level in less than 3 h, after performing a membrane-based preconcentration step, was also demonstrated.
authors: Neurochemistry and Neuroimaging Group, Laboratory of Sensors, Biosensors and Materials, Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain; Atlántica Biomédica S.L., Tenerife, Spain.
authors: Miriam
authors: M
authors: Martín
authors: Neurochemistry and Neuroimaging Group, Laboratory of Sensors, Biosensors and Materials, Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain; Informática y Equipamiento Médico de Canarias S.A., Tenerife, Spain; Laboratory of Nanotechnology on Surfaces, Institute of Materials Science of Seville (CSIC-Univ. Sevilla), Calle Américo Vespucio 49, 41092, Sevilla, Spain. Electronic address: psalazar@icmse.csic.es.
authors: Pedro
authors: P
authors: Salazar
authors: Neurochemistry and Neuroimaging Group, Laboratory of Sensors, Biosensors and Materials, Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain; Atlántica Biomédica S.L., Tenerife, Spain.
authors: Carmen
authors: C
authors: Jiménez
authors: Department of Microbiology and Infection Control, Hospital Universitario de Canarias, Tenerife, Spain.
authors: María
authors: M
authors: Lecuona
authors: Department of Microbiology and Infection Control, Hospital Universitario de Canarias, Tenerife, Spain.
authors: Mª José
authors: MJ
authors: Ramos
authors: Unit Service of Microbiology, Hospital Universitario Nuestra Señora de La Candelaria, Tenerife, Spain.
authors: Jesús
authors: J
authors: Ode
authors: Unit Service of Microbiology, Hospital Universitario Nuestra Señora de La Candelaria, Tenerife, Spain.
authors: Julia
authors: J
authors: Alcoba
authors: Informática y Equipamiento Médico de Canarias S.A., Tenerife, Spain.
authors: Rossany
authors: R
authors: Roche
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors: Reynaldo
authors: R
authors: Villalonga
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors: Susana
authors: S
authors: Campuzano
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors: José Manuel
authors: JM
authors: Pingarrón
authors: Neurochemistry and Neuroimaging Group, Laboratory of Sensors, Biosensors and Materials, Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain.
authors: José Luis
authors: JL
authors: González-Mora
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007211:Indoles / Q000737:chemistry*; D016952:Legionella pneumophila / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000175:diagnosis / Q000382:microbiology*; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D007211:Indoles; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine
keywords: Electrochemical immunoassay; Legionella pneumophila; Magnetic nanoparticles; poly(dopamine)