title: Reduction of viral load in whitefly (Bemisia tabaci Gen.) feeding on RNAi-mediated bean golden mosaic virus resistant transgenic bean plants.
abstract: The RNAi concept was explored to silence the rep gene from the bean golden mosaic virus (BGMV) and a genetically modified (GM) bean immune to the virus was previously generated. We investigated if BGMV-viruliferous whiteflies would reduce viral amount after feeding on GM plants. BGMV DNA amount was significantly reduced in whiteflies feeding in GM-plants (compared with insects feeding on non-GM plants) for a period of 4 and 8 days in 52% and 84% respectively.
authors: Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900 Brasília, DF, Brazil; Universidade de Brasília, Departamento de Biologia Celular, Campus Universitário, 70910-900 Brasília,DF, Brazil.
authors: Nayhanne T
authors: NT
authors: de Paula
authors: Embrapa Arroz e Feijão, Rodovia GO-462, km 12 Zona Rural C.P. 179, 75375-000 Santo Antônio de Goiás, GO, Brazil.
authors: Josias C
authors: JC
authors: de Faria
authors: Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900 Brasília, DF, Brazil. Electronic address: francisco.aragao@embrapa.br.
authors: Francisco J L
authors: FJ
authors: Aragão
mesh_terms: D000818:Animals; D005247:Feeding Behavior; D017791:Geminiviridae / Q000302:isolation & purification*; D006430:Hemiptera / Q000502:physiology* / Q000821:virology*; D027805:Phaseolus / Q000276:immunology* / Q000821:virology; D030821:Plants, Genetically Modified / Q000276:immunology* / Q000821:virology; D019562:Viral Load
keywords: Bean golden mosaic virus; GM plants; Viral load; Virus resistance; Whitefly