title: Productivity of superovulated C57BL/6J oocyte donors at different ages.
abstract: Superovulation is often used to increase the number of oocytes that can be collected from donor females for in vitro fertilization. Donor age can affect the quantity and quality of oocytes produced during superovulation, and in some strains of mice juvenile females are optimal donors. The authors reviewed donor and oocyte records from a breeding program to evaluate how donor age affects the number and fertilization efficiency of oocytes collected from C57BL/6J mice. Generally fewer oocytes per donor were collected from females aged > 32 d than from females aged 21-32 d. Fertilization efficiency of oocytes generally declined with donor age when oocytes were fertilized with fresh or with stored sperm. These findings suggest that the use of younger C57BL/6J donors, instead of older donors, can reduce the number of donors needed for IVF procedures.
authors: Biomodels Austria, University of Veterinary Medicine, Vienna, Austria.|Department für Agrobiotechnology, University of Natural Resources and Life Sciences, Tulln, Austria.
authors: Thomas
authors: T
authors: Kolbe
authors: Transgenic Technologies Charité, Berlin, Germany.
authors: Adrian
authors: A
authors: Landsberger
authors: Transgenic Technologies Charité, Berlin, Germany.
authors: Sabine
authors: S
authors: Manz
authors: Transgenic Technologies Charité, Berlin, Germany.
authors: Ellen
authors: E
authors: Na
authors: Transgenic Technologies Charité, Berlin, Germany.
authors: Iris
authors: I
authors: Urban
authors: Transgenic Technologies Charité, Berlin, Germany.
authors: Geert
authors: G
authors: Michel
mesh_terms: D000367:Age Factors; D000818:Animals; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D013480:Superovulation; D014019:Tissue Donors