title: PEBP4 promoted the growth and migration of cancer cells in pancreatic ductal adenocarcinoma.
abstract: Pancreatic ductal adenocarcinoma (PDAC) is one of the most common malignancies in the world. Numerous studies have linked the activation of AKT to the progression of PDAC. Phosphatidylethanolamine-binding protein 4 (PEBP4) has been reported to be upregulated in various cancer types. However, its expression pattern and biological functions in PDAC are unknown. In this study, it was found that the messenger RNA (mRNA) and protein level of PEBP4 was elevated in PDAC samples. Forced expression of PEBP4 in PDAC cell lines promoted cell growth and migration, while downregulation of PEBP4 in PDAC cells by RNA interference (RNAi) inhibited the growth, migration, and metastasis of the cancer cells. PEBP4 interacted with AKT and promoted the phosphorylation of serine 473 in AKT. Collectively, this study suggested that PEBP4 might promote the progression of PDAC through activating AKT signaling and PEBP4 might be a promising therapeutic target for PDAC treatment.
authors: General Surgery Department, The Fifth People's Hospital of Shanghai, Fudan University, 128 Ruili Rd., Shanghai, 200240, China.
authors: Dexiang
authors: D
authors: Zhang
authors: Department of Medical Oncology, Cancer Hospital of Fudan University, Minhang Branch, Shanghai, 200240, China.
authors: Yuedi
authors: Y
authors: Dai
authors: General Surgery Department, The Fifth People's Hospital of Shanghai, Fudan University, 128 Ruili Rd., Shanghai, 200240, China.
authors: Yuankun
authors: Y
authors: Cai
authors: General Surgery Department, General Surgery Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, China.
authors: Tao
authors: T
authors: Suo
authors: General Surgery Department, General Surgery Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, China.
authors: Han
authors: H
authors: Liu
authors: General Surgery Department, General Surgery Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, China.
authors: Yueqi
authors: Y
authors: Wang
authors: General Surgery Department, The Fifth People's Hospital of Shanghai, Fudan University, 128 Ruili Rd., Shanghai, 200240, China. chengzhijian2015@tom.com.
authors: Zhijian
authors: Z
authors: Cheng
authors: General Surgery Department, General Surgery Institute, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, China. liuhoubao66@sina.com.
authors: Houbao
authors: H
authors: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation / Q000502:physiology; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D051379:Mice; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D049488:Phosphatidylethanolamine Binding Protein / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C518018:PE-binding protein 4, human; D049488:Phosphatidylethanolamine Binding Protein
keywords: AKT; Cell growth and migration; PDAC; PEBP4