title: Inntags: small self-structured epitopes for innocuous protein tagging.
abstract: Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.
authors: Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
authors: Maya V
authors: MV
authors: Georgieva
authors: Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.|Department of Microbiology and Immunology, School of Pharmacy, Zagazig University, Zagazig, Egypt.
authors: Galal
authors: G
authors: Yahya
authors: Barcelona Supercomputing Center (BSC), Barcelona, Spain.|Joint BSC-CRG-IRB Programme in Computational Biology, Barcelona, Spain.
authors: Laia
authors: L
authors: Codó
authors: Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
authors: Raúl
authors: R
authors: Ortiz
authors: AbBcn Ltd, Bellaterra, Spain.
authors: Laura
authors: L
authors: Teixidó
authors: Immunostep Ltd, Salamanca, Spain.
authors: José
authors: J
authors: Claros
authors: Immunostep Ltd, Salamanca, Spain.
authors: Ricardo
authors: R
authors: Jara
authors: AbBcn Ltd, Bellaterra, Spain.
authors: Mònica
authors: M
authors: Jara
authors: AbBcn Ltd, Bellaterra, Spain.
authors: Antoni
authors: A
authors: Iborra
authors: Barcelona Supercomputing Center (BSC), Barcelona, Spain.|Joint BSC-CRG-IRB Programme in Computational Biology, Barcelona, Spain.|Departament de Bioquimica, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
authors: Josep Lluís
authors: http://orcid.org/0000-0002-0566-7723
authors: JL
authors: Gelpí
authors: Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
authors: Carme
authors: C
authors: Gallego
authors: Joint BSC-CRG-IRB Programme in Computational Biology, Barcelona, Spain.|Departament de Bioquimica, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.|Institute for Research in Biomedicine (IRB Barcelona), Barcelona, Spain.
authors: Modesto
authors: M
authors: Orozco
authors: Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
authors: Martí
authors: M
authors: Aldea
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000032:analysis* / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D011506:Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins