title: Comparative analysis of DNA methylation changes in two rice genotypes under salt stress and subsequent recovery.
abstract: DNA methylation, which is one of the best understood epigenetic phenomena, plays an important role in plant responses to environmental stimuli. The rice introgression line IL177-103 and its recurrent parent IR64, which show contrasting salt stress tolerance, were used to characterize DNA methylation changes under salt stress and subsequent recovery using methylation-sensitive amplified polymorphism (MSAP) analysis. The introgression line IL177-103 showed significantly improved tolerance to salinity, as represented by higher relative water content, endogenous abscisic acid content, activity of reactive oxygen species scavenging enzymes, and lower Na(+) concentration in shoots, compared with IR64. The MSAP results showed that less than 10.5% of detected DNA methylation sites were genotype specific, in line with their similar genetic background. Salt-induced DNA methylation changes in both genotypes were mostly detected in roots, and the major portion of the salt-induced DNA demethylation/methylation alterations remained even after recovery, implying their inheritance in the present generation. Furthermore, a few sites with stable DNA methylation differences were identified between salt-tolerant IL177-103 and salt-sensitive IR64, thus providing genotype-specific epigenetic markers. Collectively, these results provide valuable data for further dissection of the molecular mechanisms of salt-stress response and tolerance in rice.
authors: Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: wsh-wang@163.com.
authors: Wensheng
authors: W
authors: Wang
authors: Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: vickyhuangfei@163.com.
authors: Fei
authors: F
authors: Huang
authors: Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: tsinemail@163.com.
authors: Qiao
authors: Q
authors: Qin
authors: Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: zhaoxiuqin@caas.cn.
authors: Xiuqin
authors: X
authors: Zhao
authors: Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Shenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. Electronic address: lizhikang@caas.cn.
authors: Zhikang
authors: Z
authors: Li
authors: Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Shenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. Electronic address: fubinying@caas.cn.
authors: Binying
authors: B
authors: Fu
mesh_terms: D019175:DNA Methylation / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D005819:Genetic Markers; D005838:Genotype; D021141:Nucleic Acid Amplification Techniques; D012275:Oryza / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics* / Q000502:physiology*; D013045:Species Specificity; D013312:Stress, Physiological
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: DNA methylation; Epigenetic marker; Rice; Salt tolerance