title: Efficient ensemble system based on the copper binding motif for highly sensitive and selective detection of cyanide ions in 100% aqueous solutions by fluorescent and colorimetric changes.
abstract: A peptide-based ensemble for the detection of cyanide ions in 100% aqueous solutions was designed on the basis of the copper binding motif. 7-Nitro-2,1,3-benzoxadiazole-labeled tripeptide (NBD-SSH, NBD-SerSerHis) formed the ensemble with Cu(2+), leading to a change in the color of the solution from yellow to orange and a complete decrease of fluorescence emission. The ensemble (NBD-SSH-Cu(2+)) sensitively and selectively detected a low concentration of cyanide ions in 100% aqueous solutions by a colorimetric change as well as a fluorescent change. The addition of cyanide ions instantly removed Cu(2+) from the ensemble (NBD-SSH-Cu(2+)) in 100% aqueous solutions, resulting in a color change of the solution from orange to yellow and a "turn-on" fluorescent response. The detection limits for cyanide ions were lower than the maximum allowable level of cyanide ions in drinking water set by the World Health Organization. The peptide-based ensemble system is expected to be a potential and practical way for the detection of submicromolar concentrations of cyanide ions in 100% aqueous solutions.
authors: Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University , Incheon 402-751, South Korea.
authors: Kwan Ho
authors: KH
authors: Jung
authors: Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University , Incheon 402-751, South Korea.
authors: Keun-Hyeung
authors: KH
authors: Lee
mesh_terms: D020816:Amino Acid Motifs; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D003124:Colorimetry; D003300:Copper / Q000737:chemistry*; D003486:Cyanides / Q000032:analysis* / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D010069:Oxadiazoles / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: C542965:7-nitrobenzoxadiazole; D002021:Buffers; D003486:Cyanides; D009842:Oligopeptides; D010069:Oxadiazoles; D012996:Solutions; D014867:Water; D003300:Copper