title: Characterization of a solvent, surfactant and temperature-tolerant laccase from Pleurotus sp. MAK-II and its dye decolorizing property.
abstract: OBJECTIVE
  Laccase is industrially important but a major challenge is the production of an ideal laccase with suitable physicochemical properties to tolerate temperature, surfactants, metal ions and solvents towards its potential application in bioremediation.
  
  
  RESULTS
  A laccase with a molecular mass of 43 kDa was purified from Pleurotus sp. MAK-II. It was optimally active at pH 4.5 and 60 °C using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as substrate. The laccase was susceptible to NaN3 and NaCN. Activity was strongly enhanced by Cu(2+), Mg(2+) and Ca(2+). The purified laccase showed stability towards various surfactants and solvents and decolorized, in the presence of violuric acid as redox mediator, the diazo dye Congo Red and the anthraquinone dye Remazol Brilliant Blue R to the extent of 96 and 72 %, respectively.
  
  
  CONCLUSIONS
  The ideal physicochemical properties of Pleurotus sp. MAK-II-derived laccase suggest that it could be effectively used in the textile dye industry.
authors: Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, 600 025, India.
authors: Arulmani
authors: A
authors: Manavalan
authors: Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, 600 025, India.
authors: Tamilvendan
authors: T
authors: Manavalan
authors: Department of Environmental Science, Periyar University, Salem, Tamil Nadu, 636011, India.
authors: Kumarasamy
authors: K
authors: Murugesan
authors: Department of Information Systems, College of Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133791, Republic of Korea.
authors: Arne
authors: A
authors: Kutzner
authors: Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, 600 025, India.
authors: Kalaichelvan P
authors: KP
authors: Thangavelu
authors: Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133791, Republic of Korea. klaus@hanyang.ac.kr.
authors: Klaus
authors: http://orcid.org/0000-0002-0027-6993
authors: K
authors: Heese
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D052160:Benzothiazoles / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D003224:Congo Red / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D008970:Molecular Weight; D020076:Pleurotus / Q000201:enzymology*; D012997:Solvents / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism*; D013696:Temperature
chemical_list: D000880:Anthraquinones; D052160:Benzothiazoles; D004396:Coloring Agents; D004791:Enzyme Inhibitors; D008670:Metals; D012997:Solvents; D013451:Sulfonic Acids; D013501:Surface-Active Agents; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D003224:Congo Red; D042845:Laccase; C024758:Remazol Brilliant Blue R
keywords: Congo Red; Laccase; Pleurotus sp.; Remazol Brilliant Blue R; Violuric acid