title: Gene expression in WAT from healthy humans and monkeys correlates with FGF21-induced browning of WAT in mice.
abstract: OBJECTIVE
  Identify a gene expression signature in white adipose tissue (WAT) that reports on WAT browning and is associated with a healthy phenotype.
  
  
  METHODS
  RNA from several different adipose depots across three species were analyzed by whole transcriptome profiling, including 1) mouse subcutaneous white fat, brown fat, and white fat after in vivo treatment with FGF21; 2) human subcutaneous and omental fat from insulin-sensitive and insulin-resistant patients; and 3) rhesus monkey subcutaneous fat from healthy and dysmetabolic individuals.
  
  
  RESULTS
  A "browning" signature in mice was identified by cross-referencing the FGF21-induced signature in WAT with the brown adipose tissue (BAT) vs. WAT comparison. In addition, gene expression levels in WAT from insulin-sensitive/healthy vs. insulin-resistant/dysmetabolic humans and rhesus monkeys, respectively, correlated with the gene expression levels in mouse BAT vs. WAT. A subset of 49 genes were identified that were consistently regulated or differentially expressed in the mouse and human data sets that could be used to monitor browning of WAT across species.
  
  
  CONCLUSIONS
  Gene expression profiles of WATs from healthy insulin-sensitive individuals correlate with those of BAT and FGF21-induced browning of WAT.
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Karni
authors: K
authors: Schlessinger
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Wenyu
authors: W
authors: Li
authors: Department of Genetics and Pharmacogenomics, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Yejun
authors: Y
authors: Tan
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Franklin
authors: F
authors: Liu
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Sandra C
authors: SC
authors: Souza
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Effie
authors: E
authors: Tozzo
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Kevin
authors: K
authors: Liu
authors: Department of Genetics and Pharmacogenomics, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: John R
authors: JR
authors: Thompson
authors: Department of Diabetes, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Liangsu
authors: L
authors: Wang
authors: Department of Genetics and Pharmacogenomics, Early Development and Discovery Sciences, Merck Research Laboratories, Merck Sharp & Dohme Corp., Kenilworth, New Jersey, USA.
authors: Eric S
authors: ES
authors: Muise
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D020869:Gene Expression Profiling; D000882:Haplorhini; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009765:Obesity / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors