title: Bacillus amyloliquefaciens AG1 biosurfactant: Putative receptor diversity and histopathological effects on Tuta absoluta midgut.
abstract: The use of biosurfactant in pest management has received much attention for the control of plant pathogens, but few studies reported their insecticidal activity. The present study describes the insecticidal activity of biosurfactant extracted from Bacillus amyloliquefaciens strain AG1. This strain produces a lipopeptide biosurfactant exhibiting an LC50 of about 180ng/cm(2) against Tuta absoluta larvae. Accordingly, the histopathologic effect of this biosurfactant on T. absoluta larvae showed serious damages of the midgut tissues including rupture and disintegration of epithelial layer and cellular vacuolization. By PCR, we showed that this biosurfactant could be formed by several lipopeptides and polyketides including iturin, fengycin, surfactin, bacyllomicin, bacillaene, macrolactin and difficidin. Binding experiment revealed that it recognized five putative receptors located in the BBMV of T. absoluta with sizes of 68, 63, 44, 30 and 19kDa. Therefore, biosurfactant AG1 hold potential for use as an environmentally friendly agent to control the tomato leaf miner.
authors: Team of Biopesticides (LPIP), Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
authors: Saoussen
authors: S
authors: Ben Khedher
authors: Team of Biopesticides (LPIP), Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
authors: Hanen
authors: H
authors: Boukedi
authors: Team of Biopesticides (LPIP), Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
authors: Olfa
authors: O
authors: Kilani-Feki
authors: Unit of Entomology (UR13A-GR09), Regional Research Center on Horticulture and Organic Agriculture (RRCHOA), University of Sousse, Chott-Mariem, 4042, Tunisia.
authors: Ikbel
authors: I
authors: Chaib
authors: Unit of Entomology (UR13A-GR09), Regional Research Center on Horticulture and Organic Agriculture (RRCHOA), University of Sousse, Chott-Mariem, 4042, Tunisia.
authors: Asma
authors: A
authors: Laarif
authors: Team of Biopesticides (LPIP), Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia. Electronic address: mesratilobna@yahoo.com.
authors: Lobna
authors: L
authors: Abdelkefi-Mesrati
authors: Team of Biopesticides (LPIP), Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
authors: Slim
authors: S
authors: Tounsi
mesh_terms: D000818:Animals; D001407:Bacillus / Q000737:chemistry*; D061046:Biological Control Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009036:Moths / Q000187:drug effects*; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D061046:Biological Control Agents; D007306:Insecticides; D055666:Lipopeptides; D013501:Surface-Active Agents
keywords: BBMVs; Bacillus amyloliquefaciens; Biosurfactant; Histology; Putative binding receptors; Tuta absoluta