title: The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model.
abstract: We examined the effect of placenta-derived MSCs (PDMSCs) injection intraregionally and intraperitoneally on healing of induced full thickness mice skin wounds; moreover, the mechanisms by which MSCs exert their effects were also studied. Sixty female mice were divided into three groups after induction of full thickness skin wound; untreated group, wounded mice were injected with MSCs derived from human placenta intraperitoneally or intraregionally. Skin biopsies were obtained 7 and 12 days after wound incision for histological examinations, detection of vascular endothelial growth factor (VEGF) by ELISA, and estimation of expression of mouse ICAM-1, Integrin β1, Integrin β3 genes and human albumin and GAPDH genes by reverse transcription polymerase chain reaction. Human placenta derived-MSCs treated groups showed accelerated wound healing than non-treated group. VEGF, Integrin β1, and Integrin β3 levels were significantly increased in the intraregionally and intraperitoneally treated mice as compared to non-treated group at day 7 after wound induction. ICAM-1 showed significant decrease in its expression in treated groups compared with non-treated group. Interestingly, the intraperitoneal MSCs injections showed better results than intraregional one. PDMSCs accelerate full thickness skin wound healing and the intraperitoneal MSCs injections are more effective than intraregional one. MSCs promote wound healing through release of proangiogenic factors as VEGF, increase healing promoting factors as integrin β1 and β3, and decrease proinflammatory cytokines as ICAM-1.
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Somia H
authors: SH
authors: Abd-Allah
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Amal S
authors: AS
authors: El-Shal
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Sally M
authors: SM
authors: Shalaby
authors: Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Eman
authors: E
authors: Abd-Elbary
authors: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Nehad F
authors: NF
authors: Mazen
authors: Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Rania R
authors: RR
authors: Abdel Kader
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D010920:Placenta / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000473:pathology*; D012879:Skin Physiological Phenomena; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A
keywords: ICAM-1; MSCs; VEGF; gene expression; integrins; wound healing