title: SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.
abstract: Myelin basic proteins (MBP) are major constituents of the myelin sheath in the central nervous system (CNS) and the peripheral nervous system (PNS). In the CNS Mbp translation occurs locally at the axon-glial contact site in a neuronal activity-dependent manner. Recently we identified the small non-coding RNA 715 (sncRNA715) as a key inhibitor of Mbp translation during transport in oligodendrocytes. Mbp mRNA localization in Schwann cells has been observed, but has not been investigated in much detail. Here we could confirm translational repression of Mbp mRNA in Schwann cells. We show that sncRNA715 is expressed and its levels correlate inversely with MBP in cultured Schwann cells and in the sciatic nerve in vivo. Furthermore we could reduce MBP protein levels in cultured Schwann cells by increasing the levels of the inhibitory sncRNA715. Our findings suggest similarities in sncRNA715-mediated translational repression of Mbp mRNA in oligodendrocytes and Schwann cells.
authors: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors: Christina
authors: C
authors: Müller
authors: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors: Nina M
authors: NM
authors: Hochhaus
authors: MRC Laboratory for Molecular Cell Biology and the UCL Cancer Institute, University College London, London, United Kingdom.
authors: Xavier
authors: X
authors: Fontana
authors: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors: Heiko J
authors: HJ
authors: Luhmann
authors: Institute of Physiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors: Robin
authors: R
authors: White
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D051379:Mice; D004676:Myelin Basic Protein / Q000235:genetics* / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism; D014176:Protein Biosynthesis; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism*; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism
chemical_list: C499047:Mbp protein, mouse; D004676:Myelin Basic Protein; D058727:RNA, Small Untranslated; C000607649:small non-coding RNA 715, mouse