title: The upregulated α-catulin expression was involved in head-neck squamous cell carcinogenesis by promoting proliferation, migration, invasion, and epithelial to mesenchymal transition.
abstract: Rho signaling component, α-catulin, is a cytoskeletal linker protein and plays an important role in apoptotic and senescence resistance, cytoskeletal reorganization, mobility, invasion, and epithelial to mesenchymal transition (EMT) of cancer cells. Here, we transfected α-catulin-expressing plasmid into head and neck squamous cell carcinoma (HNSCC) cell and examined the phenotypes and relevant molecules. α-catulin expression was detected on tissue microarray containing squamous epithelium, dysplasia, and cancer of head and neck by immunohistochemistry. It was found that α-catulin overexpression resulted in faster growth, migration and invasion, lower apoptosis, G2/M progression, and EMT than the mock and control (P < 0.05). α-catulin overexpression increased the expression of Cyclin E1, cdc2, survivin, Bcl-2, MMP-2, MMP-9, and N-cadherin but decreased the expression of Caspase-3 and E-cadherin by real-time PCR (P < 0.05). α-catulin expression was stronger in primary cancers than those in normal squamous epithelium and dysplasia (P < 0.05), but not correlated with aggressive behaviors or adverse prognosis of HNSCC patients (P > 0.05). Multivariate survival analysis showed that distant metastasis and TNM staging were independent prognostic factors for overall survival of the HNSCC patients (P < 0.05). These data indicated that upregulated expression of α-catulin protein might have impact on the tumorigenesis of HNSCC possibly by reducing apoptosis, enhancing proliferation, cell cycle progression, migration, invasion, and EMT. It might be regarded as a potential marker for head and neck carcinogenesis or a target of gene therapy for HNSCCs.
authors: Department of Stomatology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Zhuo
authors: Z
authors: Zhang
authors: Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Xue-Feng
authors: XF
authors: Yang
authors: The Second Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Ke-Qiang
authors: KQ
authors: Huang
authors: Department of Postgraduate, Liaoning Medical University, Jinzhou, 121001, China.
authors: Li
authors: L
authors: Ren
authors: Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Shuang
authors: S
authors: Zhao
authors: Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Wen-Feng
authors: WF
authors: Gou
authors: Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Dao-Fu
authors: DF
authors: Shen
authors: Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.
authors: Hong-Zhi
authors: HZ
authors: Sun
authors: School of Health Science, Tokyo University of Technology, Nishi-Kamata 5-23-22, Ohta-ku, Tokyo, 144-8535, Japan.
authors: Yasuo
authors: Y
authors: Takano
authors: Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China. zheng_huachuan@hotmail.com.
authors: Hua-Chuan
authors: HC
authors: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D046888:Tissue Array Analysis; D014162:Transfection; D015854:Up-Regulation; D055815:Young Adult; D051178:alpha Catenin / Q000378:metabolism*
chemical_list: C495274:CTNNAL1 protein, human; D051178:alpha Catenin
keywords: Head and neck squamous cell carcinoma; Pathogenesis; Prognosis; Progression; α-Catulin