title: Molecular Survey for the Invasive Leafminer Pest Liriomyza huidobrensis (Diptera: Agromyzidae) in California Uncovers Only the Native Pest Liriomyza langei.
abstract: Liriomyza huidobrensis (Blanchard) is a highly destructive invasive leafminer pest currently causing extensive damage to vegetable and horticultural crops around the world. Liriomyza langei Frick is a leafminer pest native to California that cannot currently be morphologically distinguished from L. huidobrensis. We used a DNA-barcoding approach, a published PCR-RFLP method, and a new multiplex PCR method to analyze 664 flies matching the morphological description of huidobrensis-langei. We found no evidence for the presence of L. huidobrensis in our extensive samples from California. In addition to the new molecular method, this work is important because it provides definitive data that the California "pea leafminer" is currently, and has probably always been, L. langei. These data will also be important in the event that the highly invasive L. huidobrensis ever becomes established.
authors: Systematic Entomology Laboratory, USDA-ARS, Bldg. 005, Rm., 137, 10300 Baltimore Ave., Beltsville, MD 20705. sonja.scheffer@ars.usda.gov.
authors: Sonja J
authors: SJ
authors: Scheffer
authors: Systematic Entomology Laboratory, USDA-ARS, Bldg. 005, Rm., 137, 10300 Baltimore Ave., Beltsville, MD 20705.
authors: Matthew L
authors: ML
authors: Lewis
authors: Plant Pest Diagnostics Branch, California Department of Food and Agriculture, 3294 Meadowview Rd., Sacramento, CA 95832.
authors: Stephen D
authors: SD
authors: Gaimari
authors: Malher County Extension, Department of Crop and Soil Science, Oregon State University, 710 SW 5th Ave., Ontario, OR 97914.
authors: Stuart R
authors: SR
authors: Reitz
mesh_terms: D000818:Animals; D002140:California; D058893:DNA Barcoding, Taxonomic; D004175:Diptera / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D019476:Insect Proteins / Q000235:genetics; D058865:Introduced Species; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D019476:Insect Proteins; D003576:Electron Transport Complex IV
keywords: crop pest; invasive species; molecular diagnostics; multiplex PCR