title: Quantifying hyperoxia-mediated damage to mammalian respiratory cilia-driven fluid flow using particle tracking velocimetry optical coherence tomography.
abstract: Oxygen supplementation [hyperoxia, increased fraction of inspired oxygen (FiO 2 )] is an indispensable treatment in the intensive care unit for patients in respiratory failure. Like other treatments or drugs, hyperoxia has a risk-benefit profile that guides its clinical use. While hyperoxia is known to damage respiratory epithelium, it is unknown if damage can result in impaired capacity to generate cilia-driven fluid flow. Here, we demonstrate that quantifying cilia-driven fluid flow velocities in the sub-100 μm/s regime (sub-0.25 in./min regime) reveals hyperoxia-mediated damage to the capacity of ciliated respiratory mucosa to generate directional flow. Flow quantification was performed using particle tracking velocimetry optical coherence tomography (PTV-OCT) in ex vivo mouse trachea. The ability of PTV-OCT to detect biomedically relevant flow perturbations in the sub-100 μm/s regime was validated by quantifying temperature- and drug-mediated modulation of flow performance in ex vivo mouse trachea. Overall, PTV-OCT imaging of cilia-driven fluid flow in ex vivo mouse trachea is a powerful and straightforward approach for studying factors that modulate and damage mammalian respiratory ciliary physiology.
authors: Yale University, Department of Diagnostic Radiology, P.O. Box 208043, New Haven, Connecticut 06520, United States.
authors: Ute A
authors: UA
authors: Gamm
authors: Yale University, Department of Biomedical Engineering, 55 Prospect Street, New Haven, Connecticut 06520, United States.
authors: Brendan K
authors: BK
authors: Huang
authors: Yale University, Department of Pediatrics, P.O. Box 208064, New Haven, Connecticut 06520, United States.
authors: Mansoor
authors: M
authors: Syed
authors: Yale University, Department of Pathology, PO Box 208023, New Haven, Connecticut 06520, United States.
authors: Xuchen
authors: X
authors: Zhang
authors: Yale University, Department of Pediatrics, P.O. Box 208064, New Haven, Connecticut 06520, United States.
authors: Vineet
authors: V
authors: Bhandari
authors: Yale University, Department of Diagnostic Radiology, P.O. Box 208043, New Haven, Connecticut 06520, United StatesbYale University, Department of Biomedical Engineering, 55 Prospect Street, New Haven, Connecticut 06520, United StatescYale University, Depar.
authors: Michael A
authors: MA
authors: Choma
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D018496:Hyperoxia; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D051379:Mice; D009093:Mucus / Q000378:metabolism*; D015203:Reproducibility of Results; D020545:Respiratory Mucosa / Q000293:injuries / Q000473:pathology* / Q000503:physiopathology*; D012212:Rheology / Q000379:methods*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*