title: Identification of amino acid residues of AcMNPV P143 protein involved in rRNA degradation and restricted viral replication in BM-N cells from the silkworm Bombyx mori.
abstract: We previously demonstrated that rRNA undergoes rapid and extensive degradation in Bombyx mori BM-N cells upon infection with AcMNPV, which is triggered by AcMNPV P143 (Ac-P143) protein. Here, we showed that six amino acid residues of Ac-P143 protein, distributing between positions 514 and 599, are involved in rRNA degradation in BM-N cells. The six residues are highly conserved among P143 proteins from AcMNPV, HycuMNPV, SeMNPV and SpltMNPV, which trigger rRNA degradation in BM-N cells upon infection, but are only partially conserved in Bm-P143 protein, which does not induce rRNA degradation in BM-N cells. We also demonstrated that substitution of only two selected residues (N565S/L578F) of Bm-P143 protein with the corresponding Ac-P143 protein residues generates a mutant Bm-P143 protein that is capable of triggering rRNA degradation in BM-N cells. These results indicate that BmNPV evolved a unique P143 protein to evade the antiviral response and allow replication in B. mori cells.
authors: Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Electronic address: q2u_q1@yahoo.co.jp.
authors: Rina
authors: R
authors: Hamajima
authors: Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Electronic address: michihir@agr.nagoya-u.ac.jp.
authors: Michihiro
authors: M
authors: Kobayashi
authors: Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Electronic address: mochiko@agr.nagoya-u.ac.jp.
authors: Motoko
authors: M
authors: Ikeda
mesh_terms: D019943:Amino Acid Substitution; D000596:Amino Acids; D000818:Animals; D012831:Bombyx; D002460:Cell Line; D004261:DNA Replication; D015870:Gene Expression; D009154:Mutation; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D020871:RNA Stability; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D012335:RNA, Ribosomal; D014764:Viral Proteins
keywords: AcMNPV; Baculovirus; BmNPV; Bombyx mori cells; P143; Translation arrest; rRNA degradation