title: The Relationship between Vascular Endothelial Growth Factor 1154G/A Polymorphism and Recurrent Implantation Failure.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between herpesvirus-associated ubiquitin-specific protease (HAUSP A/G, rs1529916), tumor protein p53 (TP53 Arg/Pro, rs1042522), leukemia inhibitory factor (LIF G/T, rs929271), glycoprotein 130 (gp130 A/T, rs1900173) and vascular endothelial growth factor (VEGF G/A, rs1570360) polymorphisms and recurrent implantation failure (RIF) in Brazilian women.
  
  
  METHODS
  A total of 120 women with RIF (i.e. those with ≥5 cleaved embryos transferred and a minimum of 2 failed in vitro fertilization/intracytoplasmic sperm injection attempts) were included. The control group involved 89 women who had experienced at least 1 live birth (without any infertility treatment). DNA was extracted from the peripheral blood of all participants, and the abovementioned single-nucleotide polymorphisms (SNPs) were genotyped by real-time polymerase chain reaction. The data were evaluated using Fisher's test.
  
  
  RESULTS
  A significant difference between the RIF and control groups was found in the VEGF gene where the GG genotype showed a 2.1-fold increased chance of not being included in the RIF group, while the presence of an A allele increased this risk 1.6-fold. No significant differences were found for the other polymorphisms.
  
  
  CONCLUSIONS
  This study showed an association between the VEGF -1154G/A polymorphism and RIF in Brazilian women.
authors: Paulista Center for Diagnosis Research and Training, Ribeirx00E3;o Preto, Brazil.
authors: Laura D
authors: LD
authors: Vagnini
authors: Adriana M
authors: AM
authors: Nascimento
authors: Maria do Carmo T
authors: Mdo C
authors: Canas
authors: Adriana
authors: A
authors: Renzi
authors: Gabriela R
authors: GR
authors: Oliveira-Pelegrin
authors: Claudia G
authors: CG
authors: Petersen
authors: Ana L
authors: AL
authors: Mauri
authors: João Batista A
authors: JB
authors: Oliveira
authors: Ricardo L R
authors: RL
authors: Baruffi
authors: Mario
authors: M
authors: Cavagna
authors: José G
authors: JG
authors: Franco
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D000483:Alleles; D001938:Brazil / Q000453:epidemiology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D016158:Genes, p53 / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D043222:Ubiquitin Thiolesterase / Q000235:genetics; D000076084:Ubiquitin-Specific Peptidase 7; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D006023:Glycoproteins; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C480482:glycoprotein 130, human; C464569:USP7 protein, human; D043222:Ubiquitin Thiolesterase; D000076084:Ubiquitin-Specific Peptidase 7