title: Analysis of Amyloid in Medullary Thyroid Carcinoma by Mass Spectrometry-Based Proteomic Analysis.
abstract: Amyloid is a characteristic histologic feature in medullary thyroid carcinomas (MTC). We utilized a novel mass spectrometry-based proteomic analysis to determine if we could identify specific proteins associated with amyloid in MTC. We studied 9 MTC (1 multiple endocrine neoplasia type 2A, 1 familial MTC, and 7 sporadic). Laser microdissection was utilized to sample the amyloid which was then trypsin digested and evaluated by liquid chromatography electrospray tandem MS (LC-MS/MS) which identified the presence of amyloidogenic proteins in all cases of MTC. High levels of calcitonin were identified in all 9 cases of MTC. Secretogranin-1 was identified in 6 of 9 MTC. Calcitonin gene-related peptide was identified in 4 of 9 cases of MTC. LC-MS/MS proteomic analysis provides a rapid, highly specific, and sensitive method for identification of the specific type of amyloid in these endocrine tumors. This approach may allow classification of different forms of endocrine amyloid present in neuroendocrine tumors.
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA. erickson.lori@mayo.edu.
authors: Lori A
authors: LA
authors: Erickson
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA.
authors: Julie A
authors: JA
authors: Vrana
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA.
authors: Jason
authors: J
authors: Theis
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA.
authors: Michael
authors: M
authors: Rivera
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA.
authors: Ricardo V
authors: RV
authors: Lloyd
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA.
authors: Ellen
authors: E
authors: McPhail
authors: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55901, USA.
authors: Jun
authors: J
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000682:Amyloid / Q000032:analysis*; D002116:Calcitonin / Q000032:analysis*; D018278:Carcinoma, Neuroendocrine / Q000737:chemistry*; D053378:Chromogranin B / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013964:Thyroid Neoplasms / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000682:Amyloid; C506134:CHGB protein, human; D053378:Chromogranin B; D002116:Calcitonin
keywords: Amyloid; Calcitonin; Endocrine; Medullary thyroid carcinoma