title: Association of DC-SIGNR Expression in Peripheral Blood Mononuclear Cells with DC-SIGNR Genotypes in HIV-1 Infection.
abstract: Dendritic cell-specific intracellular adhesion molecule 3 grabbing nonintegrin related molecule (DC-SIGNR) is a C-type lectin, calcium-dependent carbohydrate-binding protein, which can act as a cell-adhesion and pathogen recognition receptor. DC-SIGNR is known to be highly expressed on liver sinusoidal cells and in the lymph nodes. However, its expression in peripheral blood mononuclear cells (PBMCs) in HIV-1 infection has not been addressed. Therefore, this study determined the expression of DC-SIGNR in PBMCs of HIV-1-infected patients and healthy seronegative individuals by real-time polymerase chain reaction and assessed its correlation with CD4+ T cell counts and DC-SIGNR genotypes. A significantly higher expression of DC-SIGNR was observed in the PBMCs of HIV-1-infected patients compared with healthy seronegative individuals. Further, there was a negative correlation between DC-SIGNR expression and CD4+ T cell counts and positive with viral load, with higher DC-SIGNR expression in the PBMCs of HIV-1-infected patients with a CD4+ T cell count <200 cells/μL than those with >200 cells/μL. This is the first study to report the expression of DC-SIGNR in PBMCs of HIV-1-infected patients. A salient finding of this study is that the DC-SIGNR expression was higher in HIV-1-infected patients, and its positive correlation with viral load and negative with CD4+ T cells counts suggesting a potential role of DC-SIGNR in HIV-1 infection.
authors: 1 Department of Biochemistry, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences , New Delhi, India .
authors: Omkar
authors: O
authors: Chaudhary
authors: 1 Department of Biochemistry, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences , New Delhi, India .
authors: Sanjeev
authors: S
authors: Kumar
authors: 2 Regional STD Teaching Training and Research Centre, Safdarjung Hospital , New Delhi, India .
authors: Manju
authors: M
authors: Bala
authors: 3 Department of Biochemistry, Kurukshetra University , Kurukshetra, India .
authors: Jasbir
authors: J
authors: Singh
authors: 4 Blood Transfusion Services, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences , New Delhi, India .
authors: Anjali
authors: A
authors: Hazarika
authors: 1 Department of Biochemistry, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences , New Delhi, India .
authors: Kalpana
authors: K
authors: Luthra
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D020869:Gene Expression Profiling; D005838:Genotype; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D037181:Lectins, C-Type / Q000096:biosynthesis* / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics*; D019562:Viral Load
chemical_list: C433029:CLEC4M protein, human; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface