title: Direct detection of the plant pathogens Burkholderia glumae, Burkholderia gladioli pv. gladioli, and Erwinia chrysanthemi pv. zeae in infected rice seedlings using matrix assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: The plant pathogens Burkholderia glumae, Burkholderia gladioli pv. gladioli, and Erwinia chrysanthemi pv. zeae were directly detected in extracts from infected rice seedlings by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method did not require culturing of the pathogens on artificial medium. In the MALDI-TOF MS analysis, peaks originating from bacteria were found in extracts from infected rice seedlings. The spectral peaks showed significantly high scores, in spite of minor differences in spectra. The spectral peaks originating from host plant tissues did not affect this direct MALDI-TOF MS analysis for the rapid identification of plant pathogens.
authors: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan. Electronic address: kajiwara@affrc.go.jp.
authors: Hideyuki
authors: H
authors: Kajiwara
mesh_terms: D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D042725:Burkholderia gladioli / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D016974:Dickeya chrysanthemi / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D012275:Oryza / Q000737:chemistry / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D036226:Seedlings / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Burkholderia gladioli pv. gladioli; Burkholderia glumae; Erwinia chrysanthemi pv. zeae; MALDI-TOF MS; Rice (Oryza sativa L.)