title: High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.
abstract: The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the screening method may reveal changes in colloidal stability of nanomaterials depending on the physicochemical conditions.
authors: Center for Molecular Protein Science, Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
authors: Irem
authors: I
authors: Nasir
authors: Upper 2nd school, Klippan, Sweden.
authors: Warda
authors: W
authors: Fatih
authors: Upper 2nd school, Klippan, Sweden.
authors: Anja
authors: A
authors: Svensson
authors: Upper 2nd school, Klippan, Sweden.
authors: Dennis
authors: D
authors: Radu
authors: Center for Molecular Protein Science, Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
authors: Sara
authors: S
authors: Linse
authors: Center for Molecular Protein Science, Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden.
authors: Celia
authors: C
authors: Cabaleiro Lago
authors: Center for Molecular Protein Science, Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden; Upper 2nd school, Klippan, Sweden.
authors: Martin
authors: M
authors: Lundqvist
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D011137:Polystyrenes; D011506:Proteins