title: Tracking the Temporal Dynamics of Intracellular Lead Speciation in a Green Alga.
abstract: Organisms have developed metal regulatory mechanisms in response to changes in the bioavailability of trace metals. Just as metal bioavailability dictates cellular uptake, intracellular metal speciation determines the availability of metals to exert biological effects. However, the missing link in understanding the relationship between metal uptake and biological responses is the ability to accurately measure intracellular metal speciation. We conducted Pb exposure studies on the well-characterized model green alga Chlamydomonas reinhardtii and identified temporal changes in intracellular Pb speciation under conditions relevant for fresh water ecosystems using resonant X-ray emission spectroscopy (RXES), which possesses enhanced sensitivity to functional group chemistry relative to X-ray absorption spectroscopy (XAS). Analysis of RXES maps show that only a small fraction of total intracellular Pb was complexed by thiol groups. Initial sequestration of Pb in oxides and inorganic phosphate was followed by binding of Pb to organic phosphate, suggesting potential interference in vital cellular functions. These results contrast proposed detoxification responses involving complexation by thiol groups from peptides.
authors: Eawag, Swiss Federal Institute of Aquatic Science and Technology , CH-8600 Dübendorf, Switzerland.|ETH , Institute of Biogeochemistry and Pollutant Dynamics IBP, CH-8092 Zurich, Switzerland.
authors: T J
authors: TJ
authors: Stewart
authors: Paul Scherrer Institute , 5232 Villigen PSI, Switzerland.|Institute of Physics, Jan Kochanowski University , P-25-406 Kielce, Poland.
authors: J
authors: J
authors: Szlachetko
authors: Eawag, Swiss Federal Institute of Aquatic Science and Technology , CH-8600 Dübendorf, Switzerland.|ETH , Institute of Biogeochemistry and Pollutant Dynamics IBP, CH-8092 Zurich, Switzerland.
authors: L
authors: L
authors: Sigg
authors: Eawag, Swiss Federal Institute of Aquatic Science and Technology , CH-8600 Dübendorf, Switzerland.
authors: R
authors: R
authors: Behra
authors: Paul Scherrer Institute , 5232 Villigen PSI, Switzerland.
authors: M
authors: M
authors: Nachtegaal
mesh_terms: D001682:Biological Availability; D002696:Chlamydomonas / Q000737:chemistry*; D042541:Intracellular Space / Q000737:chemistry*; D007854:Lead / Q000302:isolation & purification*; D013052:Spectrometry, X-Ray Emission; D013997:Time Factors
chemical_list: D007854:Lead