title: Exploring diagnostic potentials of radioiodinated sennidin A in rat model of reperfused myocardial infarction.
abstract: Non-invasive "hot spot imaging" and localization of necrotic tissue may be helpful for definitive diagnosis of myocardial viability, which is essential for clinical management of ischemic heart disease. We labeled Sennidin A (SA), a naturally occurring median dianthrone compound, with (131)I and evaluated (131)I SA as a potential necrosis-avid diagnostic tracer agent in rat model of reperfused myocardial infarction. Magnetic resonance imaging (MRI) was performed to determine the location and dimension of infarction. (131)I-SA was evaluated in rat model of 24-hour old reperfused myocardial infarction using single-photon emission computed tomography/computed tomography (SPECT/CT), biodistribution, triphenyltetrazolium chloride (TTC) histochemical staining, serial sectional autoradiography and microscopy. Gamma counting revealed high uptake and prolonged retention of (131)I SA in necrotic myocardium and fast clearance from non-targeted tissues. On SPECT/CT images, myocardial infarction was persistently visualized as well-defined hotspots over 24h, which was confirmed by perfect matches of images from post-mortem TTC staining and autoradiography. Radioactivity concentration in infarcted myocardium was over 9 times higher than that of the normal myocardium at 24h. With favorable hydrophilicity and stability, radioiodinated SA may serve as a necrosis-avid diagnostic agent for assessment of myocardial viability.
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Cuihua
authors: C
authors: Jiang
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Meng
authors: M
authors: Gao
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Yue
authors: Y
authors: Li
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Dejian
authors: D
authors: Huang
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Nan
authors: N
authors: Yao
authors: Bijie Institute of Traditional Chinese Medicine, Bijie 551700, Guizhou Province, PR China.
authors: Yun
authors: Y
authors: Ji
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Xuejiao
authors: X
authors: Liu
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Dongjian
authors: D
authors: Zhang
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors: Xiaoning
authors: X
authors: Wang
authors: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, PR China.
authors: Zhiqi
authors: Z
authors: Yin
authors: College of Sciences, Nanjing Tech University, Nanjing, Jiangsu Province, PR China.
authors: Su
authors: S
authors: Jing
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China; Faculty of Medicine, KU Leuven, Herestraat 49, Leuven 3000, Belgium.
authors: Yicheng
authors: Y
authors: Ni
authors: Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China. Electronic address: zjwonderful@hotmail.com.
authors: Jian
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000378:metabolism* / Q000493:pharmacokinetics*; D001345:Autoradiography; D004195:Disease Models, Animal; D004355:Drug Stability; D057927:Hydrophobic and Hydrophilic Interactions; D007457:Iodine Radioisotopes / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000000981:diagnostic imaging* / Q000473:pathology; D009336:Necrosis / Q000000981:diagnostic imaging; D011859:Radiography; D051381:Rats; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D000873:Anthracenes; D007457:Iodine Radioisotopes; C013946:dihydroxydianthrone
keywords: Biodistribution; Hypericin (PubChem CID: 5281051); Necrosis avidity; Radioiodinated Sennidin A; Reperfused myocardial infarction; SPECT; Sennidin A (PubChem CID: 122839)