title: The anthelmintic drug mebendazole inhibits growth, migration and invasion in gastric cancer cell model.
abstract: The present study aimed to investigate the effects of MBZ on a human malignant ascites cell line derived from a primary gastric cancer tumor. Our data reveal that MBZ showed high cytotoxicity in vitro, displaying an IC50 of 0.39 μM and 1.25 μM in ACP-02 and ACP-03, respectively. The association between MBZ and 5-FU increased slightly the cytotoxicity when compared to MBZ and 5-FU alone. Furthermore, MBZ disrupted the microtubule structure of AGP-01 cells and inhibited significantly the invasion and migration of these cells. Activity of active MMP-2 significantly decreased at all tested concentration of MBZ compared to negative control. These results support the indication of MBZ in combination with chemotherapeutic agents as a possible adjuvant therapy for the management/treatment of patients with advanced gastric cancer since MBZ is a drug of low cost with acceptable safety profile and reduced toxicity to normal cells. However, clinical trials must be performed in o to evaluate its efficacy in gastric cancer patients.
authors: Biological Science Institute, Federal University of Para, Augusto Correa Avenue, 01 Guamá, Belém, Pará, Brazil.
authors: Laine Celestino
authors: LC
authors: Pinto
authors: Biological Science Institute, Federal University of Para, Augusto Correa Avenue, 01 Guamá, Belém, Pará, Brazil.
authors: Bruno Moreira
authors: BM
authors: Soares
authors: Health Sciences Institute, Federal University of Para, Augusto Correa Avenue, 01, Guamá, Belém, Pará, Brazil.
authors: João de Jesus Viana
authors: Jde J
authors: Pinheiro
authors: Department of Neurosurgery, Johns Hopkins University School of Medicine, 1550 Orleans Street, Baltimore, MD 21231, USA.
authors: Gregory J
authors: GJ
authors: Riggins
authors: Biological Science Institute, Federal University of Para, Augusto Correa Avenue, 01 Guamá, Belém, Pará, Brazil.
authors: Paulo Pimentel
authors: PP
authors: Assumpção
authors: Biological Science Institute, Federal University of Para, Augusto Correa Avenue, 01 Guamá, Belém, Pará, Brazil.
authors: Rommel Mário Rodriguez
authors: RM
authors: Burbano
authors: Biological Science Institute, Federal University of Para, Augusto Correa Avenue, 01 Guamá, Belém, Pará, Brazil. Electronic address: rcm.montenegro@gmail.com.
authors: Raquel Carvalho
authors: RC
authors: Montenegro
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008463:Mebendazole / Q000494:pharmacology*; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000188:drug therapy*; D050257:Tubulin Modulators / Q000494:pharmacology*
chemical_list: D050257:Tubulin Modulators; D008463:Mebendazole; D020778:Matrix Metalloproteinase 2
keywords: Invasion; Mebendazole; Metalloproteinase; Migration