title: One-Step Multiplex PCR Assay for Differentiating Proposed New Species "Clostridium neonatale" from Closely Related Species.
abstract: "Clostridium neonatale" sp. nov., previously involved in an outbreak of neonatal necrotizing enterocolitis, was recently proposed as a new species of the Clostridium genus sensu stricto. We developed a one-step multiplex colony PCR for C. neonatale identification and investigated C. neonatale intestinal colonization frequency in healthy preterm neonates.
authors: EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, DHU Risques et Grossesse, Sorbonne Paris Cité, Paris, France.
authors: Laurent
authors: L
authors: Ferraris
authors: EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, DHU Risques et Grossesse, Sorbonne Paris Cité, Paris, France.
authors: Sophia
authors: S
authors: Schönherr
authors: Institut Pasteur, Unité des Bactéries Anaérobies et Toxines, Paris, France.
authors: Philippe
authors: P
authors: Bouvet
authors: Andromas SAS, Paris, France.
authors: Brunhilde
authors: B
authors: Dauphin
authors: Institut Pasteur, Unité des Bactéries Anaérobies et Toxines, Paris, France.
authors: Michel
authors: M
authors: Popoff
authors: EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, DHU Risques et Grossesse, Sorbonne Paris Cité, Paris, France.
authors: Marie Jose
authors: MJ
authors: Butel
authors: EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, DHU Risques et Grossesse, Sorbonne Paris Cité, Paris, France julio.aires@parisdescartes.fr.
authors: Julio
authors: J
authors: Aires
mesh_terms: D002170:Canada / Q000453:epidemiology; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D007422:Intestines / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S