title: In vivo repair of rat transected sciatic nerve by low-intensity pulsed ultrasound and induced pluripotent stem cells-derived neural crest stem cells.
abstract: OBJECTIVE
  To evaluate the effects of the combination of low-intensity pulsed ultrasound (LIPUS) and induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) on the regeneration of rat transected sciatic nerve in vivo.
  
  
  RESULTS
  Tissue-engineered tubular nerve conduit was fabricated by electrospinning aligned nanofibers in longitudinal direction. This sustained the iPSCs-NCSCs and could be used as a bridge in rat transected sciatic nerve. Treatment with 0.3 W cm(-2) LIPUS for 2 weeks and 5 min per day significantly improved the sciatic functional index, static sciatic function index and nerve conduction velocity of rat sciatic nerve. Histological analysis showed that there were more regenerative new blood vessels and new neurofilaments, higher expression level of β-III tubulin (Tuj1) in the experimental group seeded with iPSCs-NCSCs and stimulated with LIPUS.
  
  
  CONCLUSIONS
  Combination of LIPUS with iPSCs-NCSCs promoted the regeneration and reconstruction of rat transected sciatic nerve and is an efficient and cost-effective method for peripheral nerve regeneration.
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. yglv@cqu.edu.cn.|'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. yglv@cqu.edu.cn.|Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, 174 Shazheng Jie, Shapingba, Chongqing, 400044, People's Republic of China. yglv@cqu.edu.cn.
authors: Yonggang
authors: Y
authors: Lv
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. 939378180@qq.com.|'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. 939378180@qq.com.
authors: Panpan
authors: P
authors: Nan
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. chenguobao1985@163.com.|'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. chenguobao1985@163.com.
authors: Guobao
authors: G
authors: Chen
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. shaziwait@126.com.|'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. shaziwait@126.com.
authors: Yongqiang
authors: Y
authors: Sha
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. xiabin321@163.com.|'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. xiabin321@163.com.
authors: Bin
authors: B
authors: Xia
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. yanglibme@cqu.edu.cn.|'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China. yanglibme@cqu.edu.cn.
authors: Li
authors: L
authors: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D048091:Guided Tissue Regeneration / Q000379:methods*; D057026:Induced Pluripotent Stem Cells / Q000637:transplantation*; D009416:Nerve Regeneration; D051381:Rats; D012584:Sciatic Nerve / Q000293:injuries*; D020426:Sciatic Neuropathy / Q000628:therapy*; D016896:Treatment Outcome; D000069453:Ultrasonic Waves
keywords: Induced pluripotent stem cells; Low-intensity pulsed ultrasound; Nerve regeneration; Sciatic nerve repair