title: A turn-on fluorescent probe for tumor hypoxia imaging in living cells.
abstract: A novel "turn-on" fluorescent probe HP for hypoxia imaging was designed and synthesized based on rhodamine B and a naphthalimide fluorophore. The fluorescence of HP is very weak owing to the FRET effect from rhodamine B to the azo-naphthalimide unit. Under hypoxia conditions, the azo-bond is reduced and the fluorescence at 581 nm enhances dramatically as a result of disintegration of the quencher structure. Verified by the cyclic voltammetry reduction potential and proposed product HPN, the probe HP could undergo the chemical and cytochrome P450 enzymatic reduction quickly. When cultured with HeLa cells, HP showed remarkable fluorescence differences at various oxygen concentrations, and the ratio of fluorescence intensity between hypoxic and normoxic cells could reach 9 fold.
authors: Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China. wpzhu@ecust.edu.cn.
authors: Qi
authors: Q
authors: Cai
authors: Tao
authors: T
authors: Yu
authors: Weiping
authors: W
authors: Zhu
authors: Yufang
authors: Y
authors: Xu
authors: Xuhong
authors: X
authors: Qian
mesh_terms: D015687:Cell Hypoxia; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes