title: Peptide-based method for detection of metastatic transformation in primary tumors of breast cancer.
abstract: Detection of metastatic activity before the onset of the actual metastasis can be a promising method to combat metastasis, the foremost cause of death in cancer. Therefore, in this work, we have developed an assay method for the detection of metastatic tumor cells in primary tumor, by using a protein of the metastatic cell signaling as the biomarker. In this assay, a peptide-based probe targeting the marker protein and a sensitive nanoparticle doped graphene nanolabel are combined to enable the detection of metastatic cells. Consequently, the metastatic cells can be specifically detected and discriminated from primary tumor cells. By applying this assay method to clinical samples of primary tumor, a low amount of metastatic activity can be detected in the tumor sites, which may suggest the activity of local metastatic transformation. So, these results may point to the prospect of using the proposed method for controlling metastatic cancer.
authors: State Key Laboratory of Pharmaceutical Biotechnology and Department of Biochemistry, Nanjing University , Nanjing 210093, China.
authors: Hao
authors: H
authors: Li
authors: State Key Laboratory of Pharmaceutical Biotechnology and Department of Biochemistry, Nanjing University , Nanjing 210093, China.
authors: Yue
authors: Y
authors: Huang
authors: Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing 210008, China.
authors: Yue
authors: Y
authors: Yu
authors: State Key Laboratory of Pharmaceutical Biotechnology and Department of Biochemistry, Nanjing University , Nanjing 210093, China.
authors: Weiwei
authors: W
authors: Li
authors: Department of Oncology, the First Affiliated Hospital of Nanjing Medical University , Nanjing 210029, China.
authors: Yongmei
authors: Y
authors: Yin
authors: State Key Laboratory of Pharmaceutical Biotechnology and Department of Biochemistry, Nanjing University , Nanjing 210093, China.|Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University , Shanghai 200444, China.
authors: Genxi
authors: G
authors: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D009362:Neoplasm Metastasis; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D015335:Molecular Probes; D010455:Peptides; D006108:Graphite