title: Effect exerted by Teriparatide upon Repair Function of β-tricalcium phosphate to ovariectomised rat's femoral metaphysis defect caused by osteoporosis.
abstract: In this study, we tested the effect of Teriparatide (PTH) in combination with β-tricalcium phosphate (β-TCP) as a bone void filler in an ovariectomised rat distal femoral metaphysis model.β-TCP is a completely resorbable synthetic calcium phosphate and the Teriparatide is a drug that can promote bone formation in the condition of osteoporosis. A critical size defect of 3mm in diameter, a through-hole bone defect, was drilled into each distal femur of the ovariectomised rats. The hole was filled with β-TCP and the rat was injected PTH Teriparatide (30μg/kg) in peritoneum 5 times per week. After 4and 8 weeks the animals were killed and the degree of bone healing analysed. In total, 60 animals were investigated. When the β-TCP and PTH were used, histological, biochemistry and histomor-phometric evaluations revealed significantly better bone healing in terms of quantity and quality of the newly formed bone. The Ovariectomised rats which suffer from femur metaphysis defect are cured by embedding β-tricalcuim phosphate and intermittently cured by parathyroid hormone (PTH).
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou 325027, Zhejiang, China.
authors: Zhou-Shan
authors: ZS
authors: Tao
authors: Endocrine & Metabolic Diseases Unit, Affiliated Hospital of Guizhou Medcial University, Guizhou 550001, China.
authors: Wan-Shu
authors: WS
authors: Zhou
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou 325027, Zhejiang, China.
authors: Kai-kai
authors: KK
authors: Tu
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou 325027, Zhejiang, China.
authors: Zheng-Liang
authors: ZL
authors: Huang
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou 325027, Zhejiang, China.
authors: Qiang
authors: Q
authors: Zhou
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou 325027, Zhejiang, China.
authors: Tao
authors: T
authors: Sun
authors: Department of Orthopaedic Surgery, Wenzhou Central Hospital, Wenzhou 325000, Zhejiang, China.
authors: Yang-Xun
authors: YX
authors: Lv
authors: Sichuan Provincial Orthopedics Hospital, No. 132 West First Section First Ring Road, Chengdu 610000, Sichuan, China.
authors: Wei
authors: W
authors: Cui
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou 325027, Zhejiang, China. Electronic address: tzs19900327@163.com.
authors: Lei
authors: L
authors: Yang
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000473:pathology*; D010024:Osteoporosis; D058866:Osteoporotic Fractures / Q000188:drug therapy / Q000473:pathology*; D010281:Parathyroid Hormone; D051381:Rats; D017207:Rats, Sprague-Dawley; D019379:Teriparatide / Q000494:pharmacology*
chemical_list: D050071:Bone Density Conservation Agents; D002130:Calcium Phosphates; D010281:Parathyroid Hormone; C485817:beta-tricalcium phosphate; D019379:Teriparatide
keywords: Bone defect; Metaphysis; Micro-CT; Osteoporosis; PTH; β-TCP