title: [Effect of Decitabine on Megakaryocyte Culture of Steroid-resistant ITP Patients].
abstract: OBJECTIVE
  To investigate the effect of decitabine and plasma of ITP patients on in vitro cultrue of megakaryocytes in bone marrow of steroid-resistant ITP patients.
  
  
  METHODS
  Bone marrow mononuclear cells were isolated from 20 steroid-resistant ITP patients, both methyl cellulose semisolid culture system (to observe and count the number of megakaryocytes colony-forming unit) and liquid culture system (to analysis the expression rate of CD41a(+) cells) were used for megakaryocyte cultrue. The experiments were divided into 4 groups according to the different components of the culture system, group A was control, group B was added with decitabine, group C with ITP plasma, group D with both decitabine and ITP plasma, and the rest of the culture components were the same in the 4 groups except the above-mentioned materials. Morphology of megakaryocytes was observed by inverted and light microscopy. The expression rate of CD41a⁺ cells in culture was analysed by flow cytometric.
  
  
  RESULTS
  Different concentration of decitabine showed different effect on megakaryocyte growth of steroid-resistant ITP patients and the optimal concentration to differentiate into megakaryocyte for bone marrow mononuclear cells is 3.0 µmol/L. Compared with group A, both megakaryocyte colony forming units (CFU) and expression rate of CD41a⁺ cells in group B were statistically significantly higher (P < 0.05). As compared with group A, the megakaryocyte colony-forming units in group C decreased with statistically significant difference, while compared with group C, the megakaryocyte colony-forming units in group D obviously increased with statistically significant difference.
  
  
  CONCLUSIONS
  Decitabine is able to induce bone marrow mononuclear cells of steroid-resistant ITP patients to differentiate into megakaryocyte and the optimal concentration is 3.0 µmol/L; ITP plasma is able to inhibit the megakaryocyte growth of steroid-resistant ITP patients.
authors: Department of Hematology, Zhuzhou Central Hospital, Zhuzhou 412007, Hunan Province, China.
authors: You-Ping
authors: YP
authors: Liao
authors: Department of Hematology, Zhuzhou Central Hospital, Zhuzhou 412007, Hunan Province, China.
authors: Yu-Xia
authors: YX
authors: Liu
authors: Department of Hematology, Zhuzhou Central Hospital, Zhuzhou 412007, Hunan Province, China.
authors: Qing-Zhao
authors: QZ
authors: Li
authors: Department of Hematology, Zhuzhou Central Hospital, Zhuzhou 412007, Hunan Province, China. E-mail: doctorhgy@163.com.
authors: Guo-Yu
authors: GY
authors: Hu
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives; D001853:Bone Marrow; D001854:Bone Marrow Cells; D000077209:Decitabine; D004351:Drug Resistance; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells; D006801:Humans; D007293:Inosine Triphosphate; D008533:Megakaryocytes; D013234:Stem Cells; D013256:Steroids
chemical_list: D013256:Steroids; D007293:Inosine Triphosphate; D000077209:Decitabine; D001374:Azacitidine