title: Antiproteinuric Effects of Green Tea Extract on Tacrolimus-Induced Nephrotoxicity in Mice.
abstract: BACKGROUND
  It has been reported that proteinuria is an early predictive marker in detection of tacrolimus (TAC) nephrotoxicity. The aim of this study was to investigate the antiproteinuric effects of green tea extract (GTE) on TAC-induced acute nephrotoxicity in mice.
  
  
  METHODS
  The mice (n = 20) were divided into 4 groups (n = 5 per group); control group mice were intraperitoneally (IP) injected with 0.9% saline, TAC group mice were IP injected with TAC 1 mg/kg, and inducible nitric oxide synthase (iNOS) inhibitor group mice were given in addition NG-nitro-L-arginine-methyl ester 12 mmol/L by subcutaneous injection. TAC-GTE group mice were given TAC by IP injection and GTE 100 mg/kg by subcutaneous injection.
  
  
  RESULTS
  The 24-hour urine protein amounts were significantly increased in TAC group mice (36.1 ± 9.9 mg/d) compared with control group mice (13.3 ± 5.4 mg/d) and significantly decreased in TAC-GTE group mice (19.1 ± 6.9 mg/d, P < .01) compared with TAC group mice. The nitric oxide (NO) production by TAC was significantly suppressed by GTE and iNOS inhibitor injection. Renal tissue malondialdehyde (MDA) level was significantly increased in the TAC group compared with the control group and was significantly decreased in the TAC-GTE group compared with that of the TAC group. The antioxidant enzyme activities of superoxide dismutase and catalase were significantly suppressed in the TAC group compared with the control group and were restored in the GTE injection group.
  
  
  CONCLUSIONS
  GTE treatment has beneficial antiproteinuric effects on TAC-induced acute renal injury in mice.
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: J H
authors: JH
authors: Back
authors: Emergency Medicine, Chonnam National University Hospital, Gwangju, Republic of Korea.
authors: H H
authors: HH
authors: Ryu
authors: Department of Pathology, Chosun University Hospital, Gwangju, Republic of Korea.
authors: R
authors: R
authors: Hong
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: S A
authors: SA
authors: Han
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: Y M
authors: YM
authors: Yoon
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: D H
authors: DH
authors: Kim
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: S J
authors: SJ
authors: Hong
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: H L
authors: HL
authors: Kim
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: J H
authors: JH
authors: Chung
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea.
authors: B C
authors: BC
authors: Shin
authors: Department of Internal Medicine, Chosun University Hospital, Gwangju, Republic of Korea. Electronic address: allergist@chosun.ac.kr.
authors: Y E
authors: YE
authors: Kwon
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000150:complications; D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011507:Proteinuria / Q000209:etiology / Q000628:therapy*; D016559:Tacrolimus / Q000633:toxicity*; D013662:Tea
chemical_list: D010936:Plant Extracts; D013662:Tea; D016559:Tacrolimus