title: Effects of neonatal inflammation on the inflammatory and oxidative profile during experimental sepsis in adult life.
abstract: The present study aimed to evaluate the long-term effects of neonatal inflammation on the inflammatory and oxidative profile during experimental sepsis in adult life. Neonatal Balb/c mice received different treatments on day 10: LPS i.p. injection (100g/kg) (nLPS) or saline i.p. injection (nSal). As adults, fear/anxiety behavior was evaluated in the elevated plus maze. The following week, saline solution or LPS was administered and, after 12h, serum (inflammatory cytokines), liver (mitochondrial complexes and oxidative stress) and adrenal gland samples (angiotensin II type 1 and 2 receptors) were collected. There was an increase in the fear/anxiety behavior in the nLPS group. Neonatal administration of LPS increased the mRNA expression of the AT1 receptor and decreased the mRNA expression of the AT2 receptor in the adrenal glands of males. The complexes II and II-III increased in the nLPS saline male group when compared to control. The LPS administration in adult females, regardless of the neonatal treatment, induced a decrease of the glutathione enzyme activity. There were no differences in the inflammatory cytokines. The results showed that neonatal inflammation influenced mitochondrial respiratory chain metabolism and angiotensin II receptors in a sex-dependent manner. Balb/c mice fear and anxiety behaviors in adulthood were programmed by early life inflammatory stress.
authors: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil.
authors: Adroaldo
authors: A
authors: Lunardelli
authors: Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil.
authors: Carolina
authors: C
authors: Luft
authors: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil.
authors: Leonardo
authors: L
authors: Pedrazza
authors: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil.
authors: Bianca Andrade
authors: BA
authors: Martha
authors: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil.
authors: Jarbas Rodrigues
authors: JR
authors: de Oliveira
authors: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil; Centro Infant, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil. Electronic address: mdonadio@pucrs.br.
authors: Márcio Vinícius Fagundes
authors: MV
authors: Donadio
mesh_terms: D000311:Adrenal Glands / Q000503:physiopathology; D000818:Animals; D000831:Animals, Newborn; D001007:Anxiety; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D005239:Fear; D005260:Female; D007249:Inflammation / Q000503:physiopathology*; D008070:Lipopolysaccharides; D008099:Liver / Q000503:physiopathology; D008297:Male; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D011945:Receptors, Angiotensin / Q000378:metabolism; D018805:Sepsis / Q000503:physiopathology*; D012727:Sex Characteristics
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011945:Receptors, Angiotensin
keywords: Angiotensin; Behavior; Cytokines; Lipopolysaccharide; Mitrochondrial complexes; Neonatal stress