title: Aging Versus Postmenopausal Osteoporosis: Bone Composition and Maturation Kinetics at Actively-Forming Trabecular Surfaces of Female Subjects Aged 1 to 84 Years.
abstract: Bone strength depends on the amount of bone, typically expressed as bone mineral density (BMD), determined by dual-energy X-ray absorptiometry (DXA), and on bone quality. Bone quality is a multifactorial entity including bone structural and material compositional properties. The purpose of the present study was to examine whether bone material composition properties at actively-forming trabecular bone surfaces in health are dependent on subject age, and to contrast them with postmenopausal osteoporosis patients. To achieve this, we analyzed by Raman microspectroscopy iliac crest biopsy samples from healthy subjects aged 1.5 to 45.7 years, paired biopsy samples from females before and immediately after menopause aged 46.7 to 53.6 years, and biopsy samples from placebo-treated postmenopausal osteoporotic patients aged 66 to 84 years. The monitored parameters were as follows: the mineral/matrix ratio; the mineral maturity/crystallinity (MMC); nanoporosity; the glycosaminoglycan (GAG) content; the lipid content; and the pyridinoline (Pyd) content. The results indicate that these bone quality parameters in healthy, actively-forming trabecular bone surfaces are dependent on subject age at constant tissue age, suggesting that with advancing age the kinetics of maturation (either accumulation, or posttranslational modifications, or both) change. For most parameters, the extrapolation of models fitted to the individual age dependence of bone in healthy individuals was in rough agreement with their values in postmenopausal osteoporotic patients, except for MMC, lipid, and Pyd content. Among these three, Pyd content showed the greatest deviation between healthy aging and disease, highlighting its potential to be used as a discriminating factor.
authors: Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of Wiener Gebietskrankenkasse (WGKK), Vienna, Austria.|Allgemeine Unfallversicherungsanstalt (AUVA) Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria.
authors: Eleftherios P
authors: EP
authors: Paschalis
authors: Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
authors: Peter
authors: P
authors: Fratzl
authors: Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of Wiener Gebietskrankenkasse (WGKK), Vienna, Austria.|Allgemeine Unfallversicherungsanstalt (AUVA) Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria.
authors: Sonja
authors: S
authors: Gamsjaeger
authors: Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of Wiener Gebietskrankenkasse (WGKK), Vienna, Austria.|Allgemeine Unfallversicherungsanstalt (AUVA) Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria.
authors: Norbert
authors: N
authors: Hassler
authors: Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of Wiener Gebietskrankenkasse (WGKK), Vienna, Austria.|Allgemeine Unfallversicherungsanstalt (AUVA) Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria.
authors: Wolfgang
authors: W
authors: Brozek
authors: Dept. of Endocrinology, Morbid Obesity and Preventive Medicine, Oslo University Hospital, Aker, Norway.
authors: Erik F
authors: EF
authors: Eriksen
authors: Genetics Unit, Shriners Hospital for Children and McGill University, Montreal, QC, Canada.
authors: Frank
authors: F
authors: Rauch
authors: Genetics Unit, Shriners Hospital for Children and McGill University, Montreal, QC, Canada.
authors: Francis H
authors: FH
authors: Glorieux
authors: Medicine and Pathology, College of Physicians and Surgeons of Columbia University, New York, NY, USA.
authors: Elizabeth
authors: E
authors: Shane
authors: Medicine and Pathology, College of Physicians and Surgeons of Columbia University, New York, NY, USA.
authors: David
authors: D
authors: Dempster
authors: Medicine and Pathology, College of Physicians and Surgeons of Columbia University, New York, NY, USA.
authors: Adi
authors: A
authors: Cohen
authors: Osteoporosis Research Center, Creighton University, Omaha, NE, USA.
authors: Robert
authors: R
authors: Recker
authors: Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of Wiener Gebietskrankenkasse (WGKK), Vienna, Austria.|Allgemeine Unfallversicherungsanstalt (AUVA) Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria.
authors: Klaus
authors: K
authors: Klaushofer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D015519:Bone Density; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007085:Ilium / Q000000981:diagnostic imaging / Q000378:metabolism*; D007223:Infant; D008875:Middle Aged; D008954:Models, Biological; D015663:Osteoporosis, Postmenopausal / Q000000981:diagnostic imaging / Q000378:metabolism*
chemical_list: D000596:Amino Acids; C015484:pyridinoline
keywords: AGING; BONE MATRIX; COLLAGEN; MATRIX MINERALIZATION; NONCOLLAGENOUS PROTEINS; OSTEOPOROSIS; RAMAN SPECTROSCOPY