title: Effects of surface reaction-type pre-reacted glass ionomer on oral biofilm formation of Streptococcus gordonii.
abstract: Streptococcus gordonii, a bacterium involved in the initial colonization of tooth surfaces, contributes to dental biofilm formation and is an important cause of infective endocarditis. This study aimed to investigate the influence of surface reaction-type pre-reacted glass ionomer (S-PRG) filler on oral bacterial growth and aggregation of S. gordonii. The effect of various concentrations of S-PRG eluate on the growth and the biofilm formation of S. gordonii and other oral microorganisms (Streptococcus mutans, Streptococcus oralis, Lactobacillus acidophilus, and Candida albicans) was assessed. In addition, the effect of S-PRG eluate on coaggregation of S. gordonii with both S. oralis and Fusobacterium nucleatum was assessed. The effect of S-PRG eluate treatment on autoaggregation of S. gordonii was also evaluated. Our results indicate that S-PRG eluate treatment reduced both for the growth and for biofilm of all organisms in a dose-dependent manner. Coaggregation of S. gordonii with both S. oralis and F. nucleatum was inhibited by S-PRG eluate, whereas autoaggregation of S. gordonii increased at certain concentrations of S-PRG eluate. These results indicate that the S-PRG filler possesses antimicrobial activity that is mediated by inhibiting growth and biofilm of oral microorganisms, and by suppressing coaggregation of S. gordonii. In addition, these findings indicate that coaggregation of S. gordonii with other bacteria is inhibited by increased autoaggregation of S. gordonii.
authors: Department of Pediatric Dentistry, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan. kisaki-s@tky.ndu.ac.jp.
authors: Kisaki
authors: K
authors: Shimazu
authors: Department of Pediatric Dentistry, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors: Riyo
authors: R
authors: Oguchi
authors: Department of Microbiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors: Yukihiro
authors: Y
authors: Takahashi
authors: Department of Microbiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors: Kiyoshi
authors: K
authors: Konishi
authors: Department of Pediatric Dentistry, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors: Hiroyuki
authors: H
authors: Karibe
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D002176:Candida albicans / Q000254:growth & development; D016967:Fusobacterium nucleatum / Q000254:growth & development; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000494:pharmacology*; D007779:Lactobacillus acidophilus / Q000254:growth & development; D008422:Materials Testing; D013054:Spectrophotometry, Atomic; D054773:Streptococcus gordonii / Q000254:growth & development*; D013295:Streptococcus mutans / Q000254:growth & development; D019533:Streptococcus oralis / Q000254:growth & development; D013499:Surface Properties
chemical_list: D005899:Glass Ionomer Cements
keywords: Bacterial growth; Biofilm; Coaggregation; S-PRG filler; Streptococcus gordonii