title: Minimizing technical variation during sample preparation prior to label-free quantitative mass spectrometry.
abstract: Sample preparation is the crucial starting point to obtain high-quality mass spectrometry data and can be divided into two main steps in a bottom-up proteomics approach: cell/tissue lysis with or without detergents and a(n) (in-solution) digest comprising denaturation, reduction, alkylation, and digesting of the proteins. Here, some important considerations, among others, are that the reagents used for sample preparation can inhibit the digestion enzyme (e.g., 0.1% sodium dodecyl sulfate [SDS] and 0.5 M guanidine HCl), give rise to ion suppression (e.g., polyethylene glycol [PEG]), be incompatible with liquid chromatography-tandem mass spectrometry (LC-MS/MS) (e.g., SDS), and can induce additional modifications (e.g., urea). Taken together, all of these irreproducible effects are gradually becoming a problem when label-free quantitation of the samples is envisioned such as during the increasingly popular high-definition mass spectrometry (HDMS(E)) and sequential window acquisition of all theoretical fragment ion spectra (SWATH) data-independent acquisition strategies. Here, we describe the detailed validation of a reproducible method with sufficient protein yield for sample preparation without any known LC-MS/MS interfering substances by using 1% sodium deoxycholate (SDC) during both cell lysis and in-solution digest.
authors: Laboratory for Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium.
authors: E
authors: E
authors: Scheerlinck
authors: Laboratory for Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium.
authors: M
authors: M
authors: Dhaenens
authors: Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
authors: A
authors: A
authors: Van Soom
authors: Department of Nutrition, Genetics, and Ethology, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
authors: L
authors: L
authors: Peelman
authors: Department of Reproductive Medicine, Ghent University Hospital, B-9000 Ghent, Belgium.
authors: P
authors: P
authors: De Sutter
authors: Laboratory for Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium.
authors: K
authors: K
authors: Van Steendam
authors: Laboratory for Pharmaceutical Biotechnology, Ghent University, B-9000 Ghent, Belgium. Electronic address: dieter.deforce@ugent.be.
authors: D
authors: D
authors: Deforce
mesh_terms: D053000:Analytic Sample Preparation Methods; D001530:Belgium; D045744:Cell Line, Tumor; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D059748:Proteolysis / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism
chemical_list: D003902:Detergents; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D003840:Deoxycholic Acid; D014357:Trypsin
keywords: HDMS(E); SDC; Sample preparation