title: Development of a novel sequential pretreatment strategy for the production of bioethanol from sugarcane trash.
abstract: A novel sequential pretreatment strategy using biodiesel industry generated waste glycerol assisted transition metal and alkali pretreatment of sugarcane trash were developed for the production of bioethanol. Various process parameters affecting pretreatment as well as hydrolysis were optimized by adopting a Taguchi design. This novel method was found to be superior when compared to conventional pretreatment strategies like acid and alkali in removing hemicelluloses and lignin and the hydrolyzate is devoid of major fermentation inhibitors like organic acids and furfurals. Physico-chemical changes of the native and the pretreated biomass were evaluated by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Under optimized hydrolysis conditions 0.796 g of reducing sugar (pentoses and hexoses) per g of dry biomass after saccharification was produced. Fermentation of the non-detoxified hydrolyzate using Saccharomyces cerevisiae produced 31.928 g of bioethanol per g of dry biomass with an efficiency of 78.89%.
authors: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors: Subbiah
authors: S
authors: Raghavi
authors: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India. Electronic address: sindhurgcb@gmail.com.
authors: Raveendran
authors: R
authors: Sindhu
authors: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors: Parameswaran
authors: P
authors: Binod
authors: Ecole Polytechnique Federale de Lausanne, Institute of Urban and Regional Sciences, GC A3, Station 18, CH-1015 Lausanne, Switzerland.
authors: Edgard
authors: E
authors: Gnansounou
authors: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors: Ashok
authors: A
authors: Pandey
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D008670:Metals / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry* / Q000187:drug effects; D012972:Sodium Hydroxide / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014866:Waste Products; D014961:X-Ray Diffraction
chemical_list: D056804:Biofuels; D008670:Metals; D014866:Waste Products; D000431:Ethanol; D012972:Sodium Hydroxide; D008031:Lignin; D005990:Glycerol
keywords: Bioethanol; Biomass; Fermentation; Hydrolysis; Pretreatment