title: Raman microscopy as a novel tool to detect endothelial dysfunction.
abstract: Raman microscopy, a label-free method with high spatial resolution, shows growing potential in various fields of medical diagnostics. Several proof-of-concept studies related to the application of Raman microscopy to detect endothelial dysfunction are summarized in this work. Both ex vivo measurements of the tissues in the murine models of endothelial pathologies, as well as in vitro investigations of the cell cultures in the context of cellular transport, drug action and inflammation processes are discussed. The future directions in application of Raman spectroscopy-based methods in such studies are also described.
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland. Electronic address: baranska@chemia.uj.edu.pl.
authors: Malgorzata
authors: M
authors: Baranska
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Agnieszka
authors: A
authors: Kaczor
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Kamilla
authors: K
authors: Malek
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Aleksandra
authors: A
authors: Jaworska
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Katarzyna
authors: K
authors: Majzner
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Emilia
authors: E
authors: Staniszewska-Slezak
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Marta Z
authors: MZ
authors: Pacia
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Grzegorz
authors: G
authors: Zajac
authors: Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Jakub
authors: J
authors: Dybas
authors: Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
authors: Ewelina
authors: E
authors: Wiercigroch
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014652:Vascular Diseases / Q000175:diagnosis / Q000378:metabolism
keywords: Diabetes; Raman imaging; SERS; Single cell analysis; Systemic hypertension