title: Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells.
abstract: BACKGROUND
  The aim of the study was to explore an effective method to induce adipose-derived stem cells (ADSCs) to differentiate into Schwann-like cells in vitro.
  
  
  METHODS
  Reagents were applied in two different ways (Dezawa inducing method and modified inducing method) in which inducers including β-mercaptoethanol (β-ME), all-trans-retinoic acid (ATRA), type I collagenase, forskolin, heregulin, basic fibroblast growth factor (BFGF) and brain-derived neurotrophic factor (BDNF) were used in different ways to induce ADSCs of rats to differentiate into Schwann-like cells. After induction, the cell morphologic characteristics and the cellular immunohistochemical staining positive rate of anti-S100 and anti-GFAP (glial fibrillary acidic protein) antibodies and the gray value of immunocytochemical dye with anti-S100 and anti-GFAP antibodies and cell activity measured by the MTT method were compared with each other to evaluate the induction effects.
  
  
  RESULTS
  Both methods can induce differentiation of ADSCs of rats into Schwann-like cells, but the cellular morphology of the modified method was more similar to Schwann cells than that of the Dezawa inducing method, there was a higher cellular immunohistochemical staining positive rate and staining grey value in immunocytochemical dye with anti-S100 and anti-GFAP antibodies, and less damage in the cell activity of the modified inducing method than that of the Dezawa inducing method.
  
  
  CONCLUSIONS
  The effect of the modified method to induce ADSCs to differentiate into Schwann-like cells in vitro is superior to that of the Dezawa inducing method.
authors: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, China.
authors: Songtao
authors: S
authors: Gao
authors: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: Yan
authors: Y
authors: Zheng
authors: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, China.
authors: Qiqing
authors: Q
authors: Cai
authors: Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: Xuejian
authors: X
authors: Wu
authors: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, China.
authors: Weitao
authors: W
authors: Yao
authors: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, China.
authors: Jiaqiang
authors: J
authors: Wang
keywords: MTT; S100; Schwann-like cells; adipose-derived stem cells; glial fibrillary acidic protein