title: Comparative studies of the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine against bovine mastitis using non-invasive mouse mastitis as a model system.
abstract: This study was undertaken to compare the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine for the prevention of mastitis using the mouse as a model system. Mice immunized with formalin-killed whole cell vaccine of S. aureus residing in a biofilm when delivered via an intramammary route produced a cell mediated immune response. Mice immunized with this biofilm vaccine showed significant reductions in colonization by S. aureus in mammary glands, severity of clinical symptoms and tissue damage in mammary glands in comparison with the mice immunized with formalin-killed whole cells of planktonic S. aureus. The planktonic vaccine administered by a subcutaneous route produced a significantly higher humoral immune response (IgG1 and IgG) than the biofilm vaccine. However, considering the host response, tissue damage, the clinical severity and colonization of S. aureus in mammary glands, the biofilm vaccine performed better in immunogenicity and protective potential when administered by the intramammary route.
authors: a Faculty of Health Sciences, School of Biomedical Sciences, Curtin Health Innovation Research Institute , Curtin University , Bentley, Perth , Australia.
authors: Jully
authors: J
authors: Gogoi-Tiwari
authors: Vincent
authors: V
authors: Williams
authors: Charlene Babra
authors: CB
authors: Waryah
authors: Karina Yui
authors: KY
authors: Eto
authors: Modiri
authors: M
authors: Tau
authors: Paul
authors: P
authors: Costantino
authors: Harish Kumar
authors: HK
authors: Tiwari
authors: Trilochan
authors: T
authors: Mukkur
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D002417:Cattle; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008414:Mastitis, Bovine / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D051379:Mice; D023421:Models, Animal; D010933:Plankton / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control; D013209:Staphylococcal Vaccines / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D013209:Staphylococcal Vaccines
keywords: Staphylococcus aureus; biofilm; mouse mastitis model; planktonic; vaccine