title: Stability of fluorescent labels in PLGA polymeric nanoparticles: Quantum dots versus organic dyes.
abstract: Polymeric nanoparticles (NPs) are currently being investigated for various therapeutic, diagnostic and drug delivery applications. The study of their interactions and fate in biological systems is frequently performed via their fluorescent labeling and following them using fluorescent microscopy. Quantum dots are proposed as stable fluorescent label and compared to other organic dyes (Nile red and DiI) in terms of their entrapment, diffusion in different aqueous or lipophilic media and photostability. In vitro transfer to hydrophilic PBS solution showed that after 8h, 4.2±2.2, 15.5±2.0 and 0.9±0.02% was released from the QDs, NR and DiI nanoparticles, respectively. However, higher diffusion rates were observed in the lipophilic medium chain triglyceride and artificial sebum for all the dyes used. Fluorescent intensity of the three different markers was found to be stable over a period of 24h. Continuous illumination with laser beam using a confocal laser scanning microscopy indicated the superior stability of quantum dots compared to the other organic dyes. Skin permeation experiments have shown that QDs were the most representative marker for the polymeric nanoparticles skin penetration.
authors: Laboratory of Pharmaceutical Engineering and Biopharmaceutics, EA4267, University of Franche-Comté, Besançon, France; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. Electronic address: mona.abdel-mottaleb@univ-fcomte.fr.
authors: Mona M A
authors: MM
authors: Abdel-Mottaleb
authors: Laboratory of Pharmaceutical Engineering and Biopharmaceutics, EA4267, University of Franche-Comté, Besançon, France. Electronic address: arnaud.beduneau@univ-fcomte.fr.
authors: Arnaud
authors: A
authors: Beduneau
authors: Laboratory of Pharmaceutical Engineering and Biopharmaceutics, EA4267, University of Franche-Comté, Besançon, France. Electronic address: yann.pellequer@univ-fcomte.fr.
authors: Yann
authors: Y
authors: Pellequer
authors: Laboratory of Pharmaceutical Engineering and Biopharmaceutics, EA4267, University of Franche-Comté, Besançon, France; Laboratory of Pharmaceutical Technology and Biopharmaceutics, University of Bonn, Bonn, Germany. Electronic address: alf.lamprecht@univ-fcomte.fr.
authors: Alf
authors: A
authors: Lamprecht
mesh_terms: D000818:Animals; D002021:Buffers; D065546:Drug Liberation; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D045663:Quantum Dots / Q000737:chemistry* / Q000378:metabolism; D012869:Skin Absorption; D012965:Sodium Chloride / Q000737:chemistry; D013552:Swine
chemical_list: D002021:Buffers; D005456:Fluorescent Dyes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012965:Sodium Chloride
keywords: Confocal microscopy; DiI; Fluorescent label; Nile red; Polymeric nanoparticles; Quantum dots