title: The exosome controls alternative splicing by mediating the gene expression and assembly of the spliceosome complex.
abstract: The exosome is a complex with exoribonuclease activity that regulates RNA surveillance and turnover. The exosome also plays a role in regulating the degradation of precursor mRNAs to maintain the expression of splicing variants. In Neurospora, the silencing of rrp44, which encodes the catalytic subunit of the exosome, changed the expression of a set of spliceosomal snRNA, snRNP genes and SR protein related genes. The knockdown of rrp44 also affected the assembly of the spliceosome. RNA-seq analysis revealed a global change in bulk splicing events. Exosome-mediated splicing may regulate alternative splicing of NCU05290, NCU07421 and the circadian clock gene frequency (frq). The knockdown of rrp44 led to an increased ratio of splicing variants without intron 6 (I-6) and shorter protein isoform small FRQ (s-FRQ) as a consequence. These findings suggest that the exosome controls splicing events by regulating the degradation of precursor mRNAs and the gene expression, assembly and function of the spliceosome.
authors: State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Lin
authors: L
authors: Zhang
authors: State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Yufeng
authors: Y
authors: Wan
authors: State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Guobin
authors: G
authors: Huang
authors: State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Dongni
authors: D
authors: Wang
authors: State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Xinyang
authors: X
authors: Yu
authors: Centre for Circadian Clocks, Medical College, Soochow University, Suzhou 215123, China.
authors: Guocun
authors: G
authors: Huang
authors: State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Jinhu
authors: J
authors: Guo
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D055354:Exosomes / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics; D009491:Neurospora / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics*; D017381:Spliceosomes / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012322:RNA Precursors; D022821:RNA Splice Sites