title: Engineering of Self-Assembled Fibronectin Matrix Protein and Its Effects on Mesenchymal Stem Cells.
abstract: Fibronectin (FN) contributes to cell adhesion, proliferation, and differentiation in various cell types. To enhance the activity of fibronectin at the sites of focal adhesion, we engineered a novel recombinant fibronectin (FNIII10) fragment connected to the peptide amphiphile sequence (PA), LLLLLLCCCGGDS. In this study, the effects of FNIII10-PA on rat mesenchymal stem cells (rMSCs) were compared with those of FNIII10. FNIII10-PA showed the prominent protein adhesion activity. In addition, FNIII10-PA showed a significantly higher effect on adhesion, proliferation, and differentiation of rMSCs than FNIII10. Taken together, the FNIII10-containing self-assembled sequence enhanced rMSCs adhesion, proliferation, and differentiation.
authors: Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Korea. yunyerang@dankook.ac.kr.|Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, Korea. yunyerang@dankook.ac.kr.
authors: Ye-Rang
authors: YR
authors: Yun
authors: Department of Biochemistry, Inha University School of Medicine, Incheon 400-712, Korea. plbhang@gmail.com.
authors: Le B Hang
authors: le BH
authors: Pham
authors: Department of Biochemistry, Inha University School of Medicine, Incheon 400-712, Korea. yieri0214@naver.com.
authors: Yie-Ri
authors: YR
authors: Yoo
authors: Department of Biochemistry, Inha University School of Medicine, Incheon 400-712, Korea. sujin2manse@naver.com.
authors: Sujin
authors: S
authors: Lee
authors: Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Korea. kimhw@dku.edu.|Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, Korea. kimhw@dku.edu.|Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 330-714, Korea. kimhw@dku.edu.
authors: Hae-Won
authors: HW
authors: Kim
authors: Department of Biochemistry, Inha University School of Medicine, Incheon 400-712, Korea. juhjang@inha.ac.kr.
authors: Jun-Hyeog
authors: JH
authors: Jang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
chemical_list: D005353:Fibronectins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins
keywords: cellular activity; fibronectin; peptide amphiphile; rat mesenchymal stem cells