title: Characteristics of PCR-SSCP and RAPD-HPCE methods for identifying authentication of Penis et testis cervi in Traditional Chinese Medicine based on cytochrome b gene.
abstract: The use of Penis et testis cervi, as a kind of precious Traditional Chinese Medicine (TCM), which is derived from dry deer's testis and penis, has been recorded for many years in China. There are abundant species of deer in China, the Penis et testis from species of Cervus Nippon and Cervus elaphusL were authentic, others species were defined as adulterant (different subspecies of deer) or counterfeits (different species). Identification of their origins or authenticity becomes a key in controlling the herbal products. A modified column chromatography was used to extract mitochondrial DNA of dried deer's testis and penis from sika deer (C. Nippon) and red deer (C. elaphusL) in addition to adulterants and counterfeits. Column chromatography requires for a short time to extract mitochondrial DNA of high purity with little damage of DNA molecules, which provides the primary structure of guarantee for the specific PCR; PCR-SSCP method showed a clear intra-specific difference among patterns of single-chain fragments, and completely differentiate Penis et testis origins from C. Nippon and C. elaphusL. RAPD-HPCE was based on the standard electropherograms to compute a control spectrum curve as similarity reference (R) among different samples. The similarity analysis indicated that there were significant inter-species differences among Penis et testis' adulterant or counterfeits. Both techniques provide a fast, simple, and accurate way to directly identify among inter-species or intra-species of Penis et testis.
authors: a School of Laboratory Medicine, Beihua University , Jilin , People's Republic of China .
authors: Mingcheng
authors: M
authors: Li
authors: a School of Laboratory Medicine, Beihua University , Jilin , People's Republic of China .
authors: Lijun
authors: L
authors: Gao
authors: a School of Laboratory Medicine, Beihua University , Jilin , People's Republic of China .
authors: Li
authors: L
authors: Qu
authors: b School of Pharmacy, Beihua University , Jilin , People's Republic of China , and.
authors: Jingyu
authors: J
authors: Sun
authors: b School of Pharmacy, Beihua University , Jilin , People's Republic of China , and.
authors: Guangxin
authors: G
authors: Yuan
authors: a School of Laboratory Medicine, Beihua University , Jilin , People's Republic of China .
authors: Wei
authors: W
authors: Xia
authors: a School of Laboratory Medicine, Beihua University , Jilin , People's Republic of China .
authors: Jiamu
authors: J
authors: Niu
authors: c Jilin Leibo Scientific Co. Ltd , Jilin , People's Republic of China.
authors: Guilian
authors: G
authors: Fu
authors: c Jilin Leibo Scientific Co. Ltd , Jilin , People's Republic of China.
authors: Lihua
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D003670:Deer / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D010413:Penis / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA / Q000379:methods*; D013737:Testis / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b
keywords: Mitochondrial cytochrome b gene; PCR-SSCP; RAPD-HCPE