title: Utilization of High-Fructose Corn Syrup for Biomass Production Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Aurantiochytrium sp. YLH70.
abstract: High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in fructose and glucose, was first tested for biomass and docosahexaenoic acid productions as a mixed carbon source by a newly isolated Aurantiochytrium sp.YLH70. After the compositions of the HFCS media were optimized, the results showed that the HFCS with additions of metal ion and vitamin at low concentrations was suitable for biomass and docosahexaenoic acid productions and the metal ion and sea salt had the most significant effects on biomass production. During the 5-l fed-batch fermentation, total HFCS containing 180 g l(-1) reducing sugar was consumed and yields of biomass, lipid, and DHA could reach 78.5, 51, and 20.1 g l(-1), respectively, at 114 h. Meanwhile, the daily productivity and the reducing sugar conversion yield for docosahexaenoic acid were up to 4.23 g l(-1)day(-1) and 0.11 g g(-1). The fatty acid profile of Aurantiochytrium sp.YLH70 showed that 46.4% of total fatty acid was docosahexaenoic acid, suggesting that Aurantiochytrium sp.YLH70 was a promising DHA producer.
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China.
authors: Xin-Jun
authors: XJ
authors: Yu
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China.
authors: Zhi-Qiang
authors: ZQ
authors: Yu
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China.
authors: Ying-Liang
authors: YL
authors: Liu
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China.
authors: Jie
authors: J
authors: Sun
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China.
authors: Jian-Yong
authors: JY
authors: Zheng
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China. hzwangzhao@163.com.
authors: Zhao
authors: Z
authors: Wang
mesh_terms: D018533:Biomass; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D066248:High Fructose Corn Syrup / Q000737:chemistry*; D058009:Stramenopiles / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D066248:High Fructose Corn Syrup; D004281:Docosahexaenoic Acids
keywords: Aurantiochytrium sp. YLH70; Docosahexaenoic acid (DHA); Fermentation; High-fructose corn syrup (HFCS); Response surface methodology (RSM)