title: [Effect of Bortezomib on Proliferation, Apoptosis and SHP-2 Gene Expression of Lymphoma Cells].
abstract: OBJECTIVE
  To investigate the effects of Bortezomib on proliferation, apoptosis and SHP-2 gene expression of lymphoma Jurkat cells and Raji cells.
  
  
  METHODS
  Methylthiazoly tetrazolium assay (MTT) was used to observe the proliferation of Jurkat cells and Raji cells treated with bortezomib in different doses. Cell apoptosis was detected by morphological examination and flow cytometry. The level of SHP-2 mRNA expression before and after the treatment with bortezomib was measured by RT-PCR.
  
  
  RESULTS
  Bortezomib could inhibit the proliferation of Jurkat and Raji cells and induce their apoptosis with time-and dose-dependent manner. After treatment with 5-100 nmol/L bortezomib, the expression of SHP-2 in Jurkat cells and Raji cells was upregulated.
  
  
  CONCLUSIONS
  Bortezomib can inhibit the proliferation and induc the apoptosis of Jurkat and Raji cells obviously, upregulate the expression of SHP-2 mRNA, suggesting that the SHP-2 may participate in regulation of bortezomib induced apoptosis of Jurkat cells and Raji cells.
authors: Department of Hematology, Xingtai People Hospital, Xingtai 054000, Hebei Province, China;
authors: Li-Cai
authors: LC
authors: Jiang
authors: Department of Hematology, The Secand People Hospital of Hebei Medical University, Shijiazhuan 050000, Hebei Province, China. E-mail: luojm2011@163.com.
authors: Jian-Min
authors: JM
authors: Luo
mesh_terms: D017209:Apoptosis; D000069286:Bortezomib; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11
chemical_list: D000069286:Bortezomib; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11