title: Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects.
abstract: OBJECTIVE
  RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells.
  
  
  RESULTS
  Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses.
  
  
  CONCLUSIONS
  The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes.
  
  
  CONCLUSIONS
  The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.
authors: Centre for Cell Engineering, Joseph Black Building, Glasgow University, G12 8QQ, UK.
authors: Hannah Winifred
authors: HW
authors: Child
authors: Instituto de Nanociencia de Aragón, University of Zaragoza, C/Mariano Esquillor s/n Zaragoza, Spain.
authors: Yulán
authors: Y
authors: Hernandez
authors: Massachusetts Institute of Technology, Institute for Medical Engineering & Science, Harvard-MIT Division for Health Sciences & Technology, Cambridge, MA 02139-4307, USA.
authors: João
authors: J
authors: Conde
authors: Integrated Microscopy Facility, Joseph Black Building, Glasgow University, G12 8QQ, UK.
authors: Margaret
authors: M
authors: Mullin
authors: UCIBIO, CIGMH, Departamento de Ciencias da Vida, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, Portugal.
authors: Pedro
authors: P
authors: Baptista
authors: Instituto de Nanociencia de Aragón, University of Zaragoza, C/Mariano Esquillor s/n Zaragoza, Spain.|Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC-Universidad de Zaragoza, c/Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors: Jesus Maria
authors: JM
authors: de la Fuente
authors: Centre for Cell Engineering, Joseph Black Building, Glasgow University, G12 8QQ, UK.
authors: Catherine Cecilia
authors: CC
authors: Berry
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D055785:Gene Knockdown Techniques; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D006046:Gold
keywords: RNAi; apoptosis; c-myc; cell cycle; cell delivery; cell proliferation; gene knockdown; gold nanoparticles; knockdown; nanoparticles; off-target effects; proliferation; siRNA