title: Studying T Cell Development in Thymic Slices.
abstract: Recently, tissue slices have been adapted to study both mouse and human T cell development. Thymic slices combine and complement the strengths of existing organotypic culture systems to study thymocyte differentiation. Specifically, the thymic slice system allows for high throughput experiments and the ability to introduce homogenous developmental intermediate populations into an environment with a well-established cortex and medulla. These qualities make thymic slices a highly versatile and technically accessible model to study thymocyte development. Here we describe methods to prepare, embed, and slice thymic lobes to study T cell development in situ.
authors: Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, 142 Life Sciences Addition, #3200, Berkeley, CA, 94720-3200, USA.
authors: Jenny O
authors: JO
authors: Ross
authors: Heather J
authors: HJ
authors: Melichar
authors: Joanna
authors: J
authors: Halkias
authors: Ellen A
authors: EA
authors: Robey
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005434:Flow Cytometry; D016591:Histocytological Preparation Techniques; D066298:In Vitro Techniques; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D060168:Thymocytes / Q000166:cytology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology* / Q000502:physiology*