title: ENVIRONMENTAL EFFECTS ON SUPEROXIDE DISMUTASE AND CATALASE ACTIVITY AND EXPRESSION IN HONEY BEE.
abstract: Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajača, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.
authors: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia.
authors: Tatjana V
authors: TV
authors: Nikolić
authors: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia.
authors: Jelena
authors: J
authors: Purać
authors: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia.
authors: Snežana
authors: S
authors: Orčić
authors: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia.
authors: Danijela
authors: D
authors: Kojić
authors: Faculty of Pharmacy, University of Belgrade, Belgrade, Republic of Serbia.
authors: Dragana
authors: D
authors: Vujanović
authors: Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Republic of Serbia.
authors: Zoran
authors: Z
authors: Stanimirović
authors: Faculty of Chemistry, University of Belgrade, Belgrade, Republic of Serbia.
authors: Ivan
authors: I
authors: Gržetić
authors: Faculty of Chemistry, University of Belgrade, Belgrade, Republic of Serbia.
authors: Konstantin
authors: K
authors: Ilijević
authors: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia.|Biosens Institute, University of Novi Sad, Novi Sad, Republic of Serbia.
authors: Branko
authors: B
authors: Šikoparija
authors: Institute for Biological Research "Siniša Stanković,", University of Belgrade, Bulevar despota Stefana, Belgrade, Republic of Serbia.
authors: Duško P
authors: DP
authors: Blagojević
mesh_terms: D000818:Animals; D001516:Bees / Q000201:enzymology* / Q000235:genetics; D002374:Catalase / Q000032:analysis*; D002947:Cities; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D020869:Gene Expression Profiling; D007221:Industry; D007854:Lead / Q000032:analysis; D018384:Oxidative Stress; D055771:Serbia; D013482:Superoxide Dismutase / Q000032:analysis*; D015032:Zinc / Q000032:analysis
chemical_list: D007854:Lead; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc
keywords: catalase; honey bee; lead; pollution; superoxide dismutase