title: The nascent-polypeptide-associated complex alpha subunit regulates the polygalacturonases expression negatively and influences the pathogenicity of Sclerotinia sclerotiorum.
abstract: Sclerotinia sclerotiorum is a necrotrophic plant-pathogenic fungus that infects more than 400 species of plants. In this study the nascent polypeptide-associated complex α subunit gene of S. sclerotiorum (SsNACα; accession No. XP_001593856.1) was cloned and characterized. The relative transcript expression of SsNACα at different morphological stages of asexual development of S. sclerotiorum were analyzed by quantitative real time PCR (qRT-PCR). RNAi-mediated gene silencing was successful for SsNACα, and the mutated strains exhibited less than 15% of the relative expression of SsNACα were obtained and used for studying the biological functions of the gene. A delay in sclerotial maturation for S. sclerotiorum was observed in the SsNACα mutants. The significant elevations for both the activities of pectin-degrading enzymes and the expression of polygalacturonase genes also were associated with the mutated strains, indicating that SsNACα could negatively influence polygalacturonases expression and modulate the pathogenicity of S. sclerotiorum.
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of ChinaSchool of Biological and Food Engineering, Suzhou University, Suzhou 234000, People's Republic of China.
authors: Xiuli
authors: X
authors: Li
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors: Min
authors: M
authors: Guo
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors: Dafeng
authors: D
authors: Xu
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors: Fangxin
authors: F
authors: Chen
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors: Huajian
authors: H
authors: Zhang
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors: Yuemin
authors: Y
authors: Pan
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors: Maomao
authors: M
authors: Li
authors: College of Plant Protection, Anhui Agricultural University, Hefei 230036, People's Republic of China gaozhimou@126.com.
authors: Zhimou
authors: Z
authors: Gao
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D029741:Brassica rapa / Q000382:microbiology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D011096:Polygalacturonase / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000382:microbiology; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D018832:Molecular Chaperones; C096099:nascent-polypeptide-associated complex; D011096:Polygalacturonase
keywords: RNA interference(Jurick II, 2007 #18); Sclerotinia sclerotiorum; nascent polypeptide associated complex; polygalacturonase