title: Cationic micelle based vaccine induced potent humoral immune response through enhancing antigen uptake and formation of germinal center.
abstract: Nanoparticles have been proven to be an effective vaccine delivery system that can boost immune responses to subunit vaccines. Herein, we developed and characterized a cationic polymeric polyethylene glycol2000-poly ϵ-caprolactone2000-polyethylenimine2000 (mPEG2000-PCL2000-g-PEI2000) micelle as a potent vaccine delivery system to boost the immune response in vivo. The micelles that we developed exhibited great antigen-loading capability and minimal cytotoxicity in vitro. Meanwhile, micelles facilitated OVA antigen uptake by dendritic cells both in vitro and in vivo. More importantly, a micelle-formulated OVA vaccine could significantly promote anti-OVA antibody production by 190-fold and potently enhance T cell proliferation and the secretion of IL-5 and IFN-γ. We attributed these effects to its ability to promote antigen uptake, antigen deposition, and germinal center formation. In conclusion, the mPEG2000-PCL2000-PEI2000 micelle that we developed has potential as potent vaccine delivery system to induce Th2 immune response.
authors: Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou 325035, PR China; Wenzhou Institute of Biomaterials and Engineering, Wenzhou 325035, PR China.
authors: Zichao
authors: Z
authors: Luo
authors: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou 325027, PR China.
authors: Shuai
authors: S
authors: Shi
authors: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou 325027, PR China.
authors: Ling
authors: L
authors: Jin
authors: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou 325027, PR China.
authors: Lu
authors: L
authors: Xu
authors: Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou 325035, PR China; Wenzhou Institute of Biomaterials and Engineering, Wenzhou 325035, PR China.
authors: Jing
authors: J
authors: Yu
authors: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou 325027, PR China. Electronic address: dragonhaochen@163.com.
authors: Hao
authors: H
authors: Chen
authors: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou 325027, PR China. Electronic address: lixingyi_1984@163.com.
authors: Xingyi
authors: X
authors: Li
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D002412:Cations / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D003713:Dendritic Cells / Q000276:immunology; D016503:Drug Delivery Systems; D005260:Female; D018858:Germinal Center / Q000187:drug effects / Q000276:immunology*; D056724:Immunity, Humoral / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008823:Micelles; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000941:Antigens; D002412:Cations; D008823:Micelles; D014612:Vaccines; D010047:Ovalbumin
keywords: Adjuvant; Germinal center; Immune response; Micelle-based vaccine