title: Umbilical cord blood markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes.
abstract: OBJECTIVE
  To determine umbilical cord blood total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), thiobarbituric acid-reacting substances (TBARS), advanced glycation end products (AGEs) and markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and their associations with microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA), funisitis and selected aspects of short-term neonatal morbidity.
  
  
  METHODS
  One hundred and sixty-five women with singleton pregnancies complicated by PPROM were included in this study. Blood samples were obtained by venipuncture from the umbilical cord vein after the delivery of the newborn. The umbilical cord blood concentrations of TAC, FRAP, TBARS and AGEs were measured.
  
  
  RESULTS
  The presence of MIAC, HCA and funisitis did not show differences in the umbilical cord blood TAC, FRAP, TBARS and AGEs concentrations. Positive correlations were found between the gestational age at sampling and umbilical cord blood TAC and AGEs concentrations (TAC: rho = 0.26; p = 0.001; AGEs: rho = 0.35; p < 0.0001). There was no association between umbilical cord blood TAC, FRAP, TBARS and AGEs concentrations and selected aspects of short-term neonatal morbidity.
  
  
  CONCLUSIONS
  Oxidative stress is associated with PPROM, as indicated by the presence of markers tested in the umbilical cord blood; however, the evaluated oxidative stress markers are not influenced by the presence of MIAC and/or HCA, and funisitis or subsequent development of selected aspects of short-term neonatal morbidity.
authors: a Department of Obstetrics and Gynecology , Charles University in Prague, Faculty of Medicine Hradec Kralove , Hradec Kralove , Czech Republic .
authors: Ivana
authors: I
authors: Musilova
authors: b Institute of Molecular Biomedicine, Comenius University in Bratislava , Bratislava , Slovak Republic .|c Center for Molecular Medicine, Slovak Academy of Sciences , Bratislava , Slovak Republic .
authors: Lubomira
authors: L
authors: Tothova
authors: d Department of Obstetrics and Gynecology , The University of Texas Medical Branch at Galveston , Galveston , TX , USA .
authors: Ramkumar
authors: R
authors: Menon
authors: b Institute of Molecular Biomedicine, Comenius University in Bratislava , Bratislava , Slovak Republic .|c Center for Molecular Medicine, Slovak Academy of Sciences , Bratislava , Slovak Republic .
authors: Barbora
authors: B
authors: Vlkova
authors: b Institute of Molecular Biomedicine, Comenius University in Bratislava , Bratislava , Slovak Republic .|c Center for Molecular Medicine, Slovak Academy of Sciences , Bratislava , Slovak Republic .
authors: Peter
authors: P
authors: Celec
authors: e Fingerland'S Department of Pathology, Charles University in Prague, Faculty of Medicine Hradec Kralove , University Hospital Hradec Kralove , Hradec Kralove , Czech Republic .
authors: Helena
authors: H
authors: Hornychova
authors: f Department of Clinical Biochemistry , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic .
authors: Radka
authors: R
authors: Kutova
authors: g Department of Clinical Immunology and Allergy , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic , and.
authors: Ctirad
authors: C
authors: Andrys
authors: a Department of Obstetrics and Gynecology , Charles University in Prague, Faculty of Medicine Hradec Kralove , Hradec Kralove , Czech Republic .
authors: Martin
authors: M
authors: Stepan
authors: a Department of Obstetrics and Gynecology , Charles University in Prague, Faculty of Medicine Hradec Kralove , Hradec Kralove , Czech Republic .|h Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
authors: Marian
authors: M
authors: Kacerovsky
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002821:Chorioamnionitis / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005322:Premature Rupture of Fetal Membranes / Q000097:blood*; D005865:Gestational Age; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D018384:Oxidative Stress; D011247:Pregnancy; D011446:Prospective Studies; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017127:Glycation End Products, Advanced; D017392:Thiobarbituric Acid Reactive Substances
keywords: Advanced glycation end products; ferric reducing antioxidant power; histological chorioamnionitis; microbial invasion of the amniotic cavity; preterm delivery; rupture of membranes; thiobarbituric acid-reacting substances; total antioxidant capacity