title: Enhancement of surface plasmon resonance signals using a MIP/GNPs/rGO nano-hybrid film for the rapid detection of ractopamine.
abstract: A novel surface plasmon resonance (SPR) sensor that uses molecular imprinted polymers (MIPs) coated with gold nanoparticles (GNPs) and reduced graphene oxide (rGO) as a sensing nano-hybrid film was developed for detection of ractopamine. The MIPs were synthesized by precipitation polymerization and characterized by scanning electron microscopy and Scatchard analysis. The GNPs/rGO composite was synthesized by a single-step reduction of graphene oxide and HAuCl4 solution. The MIP/GNPs/rGO nano-hybrid film was immobilized onto a bare sensor chip and exhibited remarkable sensitivity and stability by the “grafting to” method with the assistance of ionic liquid (IL) as a binder. The prepared sensor showed class-specific selectivity for ractopamine (RAC) and its analogs under optimized conditions. The novel SPR sensor had a wide linear range over an RAC concentration from 20 to 1000 ng/mL with a detection limit of 5 ng/mL (S/N=3). The results demonstrated that the MIP/GNPs/rGO nano-hybrid film was suitable as the recognition element of the SPR sensorfor rapid screening and detection of beta-agonists such as RAC.
authors: Ting
authors: T
authors: Yao
authors: Xu
authors: X
authors: Gu
authors: Tengfei
authors: T
authors: Li
authors: Junguo
authors: J
authors: Li
authors: Jun
authors: J
authors: Li
authors: Zhen
authors: Z
authors: Zhao
authors: Jing
authors: J
authors: Wang
authors: Yuchang
authors: Y
authors: Qin
authors: Yongxin
authors: Y
authors: She
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles; D054802:Molecular Imprinting; D010087:Oxides / Q000737:chemistry; D010627:Phenethylamines / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D010087:Oxides; D010627:Phenethylamines; D011108:Polymers; C060897:ractopamine; D006046:Gold; D006108:Graphite