title: Lepidotol A from Mesua lepidota Inhibits Inflammatory and Immune Mediators in Human Endothelial Cells.
abstract: Phytochemical investigation on the fruits of Mesua lepidota (Calophyllaceae) led to the isolation of seven new phenylcoumarin derivatives named lepidotols A-E (1-5) and lepidotins A and B (6, 7). These structures were elucidated by spectroscopic and spectrometric methods including UV, NMR, and HRMS. Lepidotol A (1), the major compound, was evaluated for its inhibitory effect on inflammation and immunity using endothelial cell-based cellular assays. At 10 μM, 1 exhibited an anti-inflammatory activity, with a significant inhibition of vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 expression induced by tumor necrosis factor-α. Lepidotol A also showed a mild immunosuppressive effect, with inhibition of the major histocompatibility complex molecules, namely, human leukocyte antigen (HLA)-DR and HLA-E.
authors: EA921 SONAS, SFR4207 QUASAV, Université d'Angers , Angers, France.
authors: Caroline
authors: C
authors: Rouger
authors: EA921 SONAS, SFR4207 QUASAV, Université d'Angers , Angers, France.
authors: Séverine
authors: S
authors: Derbré
authors: INSERM UMR1064, Centre de Recherche en Transplantation et Immunologie , LabEx IGO and LabEx Transplantex, Nantes, France.|CHU de Nantes, Institut de Transplantation-Urologie-Néphrologie, ITUN , Nantes, France.|Faculté de Médecine, LUNAM, Université de Nantes , Nantes, France.
authors: Béatrice
authors: B
authors: Charreau
authors: INSERM UMR1064, Centre de Recherche en Transplantation et Immunologie , LabEx IGO and LabEx Transplantex, Nantes, France.|CHU de Nantes, Institut de Transplantation-Urologie-Néphrologie, ITUN , Nantes, France.|Faculté de Médecine, LUNAM, Université de Nantes , Nantes, France.
authors: Angélique
authors: A
authors: Pabois
authors: Laboratoire MOLTECH-Anjou, CNRS UMR6200, Université d'Angers , Angers, France.
authors: Thomas
authors: T
authors: Cauchy
authors: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles (ICSN) , CNRS, LabEx CEBA, Gif-sur-Yvette, France.
authors: Marc
authors: M
authors: Litaudon
authors: Department of Chemistry, Faculty of Science, University of Malaya , Kuala Lumpur, Malaysia.
authors: Khalijah
authors: K
authors: Awang
authors: EA921 SONAS, SFR4207 QUASAV, Université d'Angers , Angers, France.
authors: Pascal
authors: P
authors: Richomme
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D007249:Inflammation / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D029581:Malpighiaceae / Q000737:chemistry*; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D000893:Anti-Inflammatory Agents; D003374:Coumarins; D007155:Immunologic Factors; D016328:NF-kappa B; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C000603554:lepidotol A; D018799:Intercellular Adhesion Molecule-1