title: Genetic Diversity of Bactrocera dorsalis (Diptera: Tephritidae) on the Hawaiian Islands: Implications for an Introduction Pathway Into California.
abstract: Population genetic diversity of the oriental fruit fly, Bactrocera dorsalis (Hendel), on the Hawaiian islands of Oahu, Maui, Kauai, and Hawaii (the Big Island) was estimated using DNA sequences of the mitochondrial cytochrome c oxidase subunit I gene. In total, 932 flies representing 36 sampled sites across the four islands were sequenced for a 1,500-bp fragment of the gene named the C1500 marker. Genetic variation was low on the Hawaiian Islands with >96% of flies having just two haplotypes: C1500-Haplotype 1 (63.2%) or C1500-Haplotype 2 (33.3%). The other 33 flies (3.5%) had haplotypes similar to the two dominant haplotypes. No population structure was detected among the islands or within islands. The two haplotypes were present at similar frequencies at each sample site, suggesting that flies on the various islands can be considered one population. Comparison of the Hawaiian data set to DNA sequences of 165 flies from outbreaks in California between 2006 and 2012 indicates that a single-source introduction pathway of Hawaiian origin cannot explain many of the flies in California. Hawaii, however, could not be excluded as a maternal source for 69 flies. There was no clear geographic association for Hawaiian or non-Hawaiian haplotypes in the Bay Area or Los Angeles Basin over time. This suggests that California experienced multiple, independent introductions from different sources.
authors: Center for Plant Health Science and Technology, Mission Laboratory, USDA-APHIS, Moore Air Base, 22675 North Moorefield Rd., Edinburg, TX 78541. norman.b.barr@aphis.usda.gov.
authors: Norman B
authors: NB
authors: Barr
authors: Center for Plant Health Science and Technology, Mission Laboratory, USDA-APHIS, Moore Air Base, 22675 North Moorefield Rd., Edinburg, TX 78541.
authors: Lisa A
authors: LA
authors: Ledezma
authors: Department of Plant and Environmental Protection Sciences, University of Hawaii, 3050 Maile Way, Honolulu, HI 96822-2271.
authors: Luc
authors: L
authors: Leblanc
authors: Department of Plant and Environmental Protection Sciences, University of Hawaii, 3050 Maile Way, Honolulu, HI 96822-2271.
authors: Michael
authors: M
authors: San Jose
authors: Department of Plant and Environmental Protection Sciences, University of Hawaii, 3050 Maile Way, Honolulu, HI 96822-2271.
authors: Daniel
authors: D
authors: Rubinoff
authors: United States Pacific Basin Agricultural Research Center, USDA- ARS, 64 Nowelo St., Hilo, HI 96720.
authors: Scott M
authors: SM
authors: Geib
authors: Hawaii Fruit Fly Detection Program, USDA-APHIS, 91-329 Kauhi St., Suite 100, Kapolei, HI 96707.
authors: Brian
authors: B
authors: Fujita
authors: Center for Plant Health Science and Technology, Mission Laboratory, USDA-APHIS, Moore Air Base, 22675 North Moorefield Rd., Edinburg, TX 78541.
authors: David W
authors: DW
authors: Bartels
authors: Center for Plant Health Science and Technology, Mission Laboratory, USDA-APHIS, Moore Air Base, 22675 North Moorefield Rd., Edinburg, TX 78541.
authors: Daniel
authors: D
authors: Garza
authors: California Department of Food and Agriculture, Plant Pest Diagnostics Branch, 3294 Meadowview Rd., Sacramento, CA 95832-1448.
authors: Peter
authors: P
authors: Kerr
authors: California Department of Food and Agriculture, Plant Pest Diagnostics Branch, 3294 Meadowview Rd., Sacramento, CA 95832-1448.
authors: Martin
authors: M
authors: Hauser
authors: California Department of Food and Agriculture, Plant Pest Diagnostics Branch, 3294 Meadowview Rd., Sacramento, CA 95832-1448.
authors: Stephen
authors: S
authors: Gaimari
mesh_terms: D063147:Animal Distribution; D000818:Animals; D002140:California; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D006254:Hawaii; D007300:Insect Control; D019476:Insect Proteins / Q000235:genetics; D058865:Introduced Species; D017422:Sequence Analysis, DNA; D033621:Tephritidae / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D003576:Electron Transport Complex IV
keywords: COI; fruit fly; molecular diagnosis; pathway analysis