title: Determinants of Proteolysis and Cell-Binding for the Shigella flexneri Cytotoxin, SigA.
abstract: Shigella flexneri secretes an enterotoxic, SPATE family autotransporter (AT), SigA, which has cytopathic activity towards cultured epithelial cells. Its cytopathic activity is due to its ability to degrade the cytoskeletal protein, α-fodrin. The mechanisms by which AT toxins target cells and tissues differ and the details of how SigA acts are not known. In the current study, the determinants of proteolysis and cell-targeting for SigA were determined. We demonstrate that the SigA passenger or α-domain consists of two functionally distinct domains, designated α1 and α2, which are sufficient to specify proteolytic and cell-binding activities, respectively.
authors: School of Pathology and Laboratory Medicine, University of Western Australia, Nedlands, WA, 6009, Australia.
authors: Eng Guan
authors: EG
authors: Chua
authors: Department of Microbiology, Monash University, Clayton, VIC, 3800, Australia.
authors: Keith
authors: K
authors: Al-Hasani
authors: Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, VIC, 3052, Australia.
authors: Martin
authors: M
authors: Scanlon
authors: Department of Microbiology, Monash University, Clayton, VIC, 3800, Australia.
authors: Ben
authors: B
authors: Adler
authors: School of Pathology and Laboratory Medicine, University of Western Australia, Nedlands, WA, 6009, Australia. h.sakellaris@griffith.edu.au.|Menzies Health Institute Queensland & School of Medical Science, Griffith University Gold Coast Campus, Gold Coast, QLD, 4222, Australia. h.sakellaris@griffith.edu.au.
authors: Harry
authors: H
authors: Sakellaris
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012763:Shigella flexneri / Q000235:genetics / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D011993:Recombinant Fusion Proteins