title: HN Protein of Newcastle Disease Virus Induces Apoptosis Through SAPK/JNK Pathway.
abstract: Many viral proteins are responsible for causing induction of apoptosis in the target cells. Hemagglutinin neuraminidase (HN), a multifunctional protein of Newcastle disease virus (NDV), is one of such proteins. The present study was undertaken to determine the apoptotic potential of the HN gene in cultured human cervical cancer cell line (HeLa cell) and to elucidate the molecular mechanisms involved. The results of the study indicate that HN protein causes apoptosis in HeLa cells, as observed by the translocation of Phosphatidylserine, activation of caspases, cleavage of poly (ADP-ribose) polymerase (PARP), and DNA fragmentation. Further, we report that expression of HN protein upregulates the SAPK/JNK pathway leading to transactivation of c-Jun which in turn activates apoptosis signaling. The results of our study provide an insight into the mechanism through which HN induces apoptosis.
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: R S
authors: RS
authors: Rajmani
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: Ravi Kumar
authors: RK
authors: Gandham
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: Shishir Kumar
authors: SK
authors: Gupta
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: A P
authors: AP
authors: Sahoo
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: Prafull Kumar
authors: PK
authors: Singh
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: Rajiv
authors: R
authors: Kumar
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: Shikha
authors: S
authors: Saxena
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India.
authors: Uttara
authors: U
authors: Chaturvedi
authors: Molecular Biology Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India. aktiwari63@yahoo.com.
authors: Ashok K
authors: AK
authors: Tiwari
mesh_terms: D017209:Apoptosis; D015707:HN Protein / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D009522:Newcastle disease virus / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D015707:HN Protein; D016898:Interferon-alpha; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Apoptosis; Caspases; Heamagglutinin neuraminidase (HN); Interferon-α (IFN-α); NDV; SAPK/JNK pathway; c-Jun