title: Effect of Colchicine on Platelet-Platelet and Platelet-Leukocyte Interactions: a Pilot Study in Healthy Subjects.
abstract: The cardioprotective mechanisms of colchicine in patients with stable ischemic heart disease remain uncertain. We tested varying concentrations of colchicine on platelet activity in vitro and a clinically relevant 1.8-mg oral loading dose administered over 1 h in 10 healthy subjects. Data are shown as median [interquartile range]. Colchicine addition in vitro decreased light transmission platelet aggregation only at supratherapeutic concentrations but decreased monocyte- (MPA) and neutrophil-platelet aggregation (NPA) at therapeutic concentrations. Administration of 1.8 mg colchicine to healthy subjects had no significant effect on light transmission platelet aggregation but decreased the extent of MPA (28 % [22-57] to 22 % [19-31], p = 0.05) and NPA (19 % [16-59] to 15 % [11-30], p = 0.01), platelet surface expression of PAC-1 (370 mean fluorescence intensity (MFI) [328-555] to 333 MFI [232-407], p = 0.02) and P-selectin (351 MFI [269-492] to 279 [226-364], p = 0.03), and platelet adhesion to collagen (10.2 % [2.5-32.6] to 2.0 % [0.2-9.5], p = 0.09) 2 h post-administration. Thus, in clinically relevant concentrations, colchicine decreases expression of surface markers of platelet activity and inhibits leukocyte-platelet aggregation but does not inhibit homotypic platelet aggregation.
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA. Binita.Shah@nyumc.org.|Department of Medicine, Section of Cardiology, Veterans Affairs New York Harbor Health Care System, New York, NY, USA. Binita.Shah@nyumc.org.
authors: Binita
authors: B
authors: Shah
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Nicole
authors: N
authors: Allen
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Bhisham
authors: B
authors: Harchandani
authors: Department of Medicine, Division of Rheumatology, New York University School of Medicine, New York, NY, USA.
authors: Michael
authors: M
authors: Pillinger
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Stuart
authors: S
authors: Katz
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.|Department of Medicine, Section of Cardiology, Veterans Affairs New York Harbor Health Care System, New York, NY, USA.
authors: Steven P
authors: SP
authors: Sedlis
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Christina
authors: C
authors: Echagarruga
authors: Department of Medicine, Division of Rheumatology, New York University School of Medicine, New York, NY, USA.
authors: Svetlana Krasnokutsky
authors: SK
authors: Samuels
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Pajazit
authors: P
authors: Morina
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Prabhjot
authors: P
authors: Singh
authors: Department of Medicine, Division of Cardiology, New York University School of Medicine, 227 E 30th Street, Office 835, New York, NY, 10016, USA.
authors: Liza
authors: L
authors: Karotkin
authors: Department of Medicine, Divisions of Cardiology and Hematology, New York University School of Medicine, New York, NY, USA.
authors: Jeffrey S
authors: JS
authors: Berger
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000188:drug therapy; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010865:Pilot Projects; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D002316:Cardiotonic Agents; D003078:Colchicine
keywords: colchicine; monocyte; neutrophil; platelet aggregation; tissue adhesion