title: The consequence of biologic graft processing on blood interface biocompatibility and mechanics.
abstract: Processing ex vivo derived tissues to reduce immunogenicity is an effective approach to create biologically complex materials for vascular reconstruction. Due to the sensitivity of small diameter vascular grafts to occlusive events, the effect of graft processing on critical parameters for graft patency, such as peripheral cell adhesion and wall mechanics, requires detailed analysis. Isolated human umbilical vein sections were used as model allogenic vascular scaffolds that were processed with either: 1. sodium dodecyl sulfate (SDS), 2. ethanol/acetone (EtAc), or 3. glutaraldehyde (Glu). Changes in material mechanics were assessed via uniaxial tensile testing. Peripheral cell adhesion to the opaque grafting material was evaluated using an innovative flow chamber that allows direct observation of the blood-graft interface under physiological shear conditions. All treatments modified the grafts tensile strain and stiffness properties, with physiological modulus values decreasing from Glu 240±12 kPa to SDS 210±6 kPa and EtAc 140±3 kPa, P<.001. Relative to glutaraldehyde treatments, neutrophil adhesion to the decellularized grafts increased, with no statistical difference observed between SDS or EtAc treatments. Early platelet adhesion (% surface coverage) showed no statistical difference between the three treatments; however, quantification of platelet aggregates was significantly higher on SDS scaffolds compared to EtAc or Glu. Tissue processing strategies applied to the umbilical vein scaffold were shown to modify structural mechanics and cell adhesion properties, with the EtAc treatment reducing thrombotic events relative to SDS treated samples. This approach allows time and cost effective prescreening of clinically relevant grafting materials to assess initial cell reactivity.
authors: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, PO Box 116131, 1275 Center Drive, Gainesville, FL 32611.
authors: Aurore B
authors: AB
authors: Van de Walle
authors: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, PO Box 116131, 1275 Center Drive, Gainesville, FL 32611.
authors: Joseph S
authors: JS
authors: Uzarski
authors: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, PO Box 116131, 1275 Center Drive, Gainesville, FL 32611.
authors: Peter S
authors: PS
authors: McFetridge
mesh_terms: D000096:Acetone / Q000494:pharmacology; D001790:Blood Physiological Phenomena / Q000187:drug effects*; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010973:Platelet Adhesiveness / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013718:Tensile Strength / Q000187:drug effects; D054457:Tissue Scaffolds; D019737:Transplants / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D014471:Umbilical Veins / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000096:Acetone; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D005976:Glutaral
keywords: biocompatibility; biomaterial; decellularization; flow chamber; human umbilical vein; thrombosis; vascular tissue engineering