title: Molecular epidemiology of VIM-1 producing Escherichia coli from Germany referred to the National Reference Laboratory.
abstract: The distribution of carbapenemase genes in Escherichia coli strains isolated between September 2009 and May 2013 in Germany was investigated. Out of 192 isolates with carbapenemase production OXA-48 was found in 44.8%, VIM-1 in 18.8%, NDM-1 in 11.5% and KPC-2 in 6.8%. Patients with VIM-1 producing E. coli (n=36) differed from patients with OXA-48 by an older age, less frequent mention of travel history and an increased proportion of clinical over screening specimens. These data might indicate that introduction from abroad is of minor importance for VIM-1 producing E. coli compared to other carbapenemases. Multilocus sequence typing revealed that E. coli with VIM-1 were mostly multiclonal, emphasizing the role of horizontal gene transfer in its spread. Susceptibility testing of VIM-1 producing E. coli demonstrated aztreonam susceptibility in 55.6%. Among non-β-lactams susceptibility rates of >90% were observed for amikacin, tigecycline, colistin, fosfomycin and nitrofurantoin.
authors: Department of Medical Microbiology, National Reference Centre for Multidrug-resistant Gram-negative Bacteria, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany. Electronic address: martin.kaase@rub.de.
authors: Martin
authors: M
authors: Kaase
authors: Department of Medical Microbiology, National Reference Centre for Multidrug-resistant Gram-negative Bacteria, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
authors: Niels
authors: N
authors: Pfennigwerth
authors: Department of Medical Microbiology, National Reference Centre for Multidrug-resistant Gram-negative Bacteria, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
authors: Felix
authors: F
authors: Lange
authors: Department of Medical Microbiology, National Reference Centre for Multidrug-resistant Gram-negative Bacteria, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
authors: Agnes
authors: A
authors: Anders
authors: Department of Medical Microbiology, National Reference Centre for Multidrug-resistant Gram-negative Bacteria, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
authors: Sören G
authors: SG
authors: Gatermann
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005260:Female; D022761:Gene Transfer, Horizontal; D014644:Genetic Variation; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D015995:Prevalence; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics
chemical_list: C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases
keywords: Carbapenemases; Enterobacteriaceae; Escherichia coli; KPC-2; NDM-1; OXA-48; VIM-1