title: Experimental Furcal Perforation Treated with MTA: Analysis of the Cytokine Expression.
abstract: The aim of this study was to evaluate mineral trioxide aggregate (MTA)-induced cytokine expression in mice after experimental furcal perforation. BALB/c mice (n=5) were subjected to induced furcal drilling of the maxillary first molar followed by MTA sealing in the left side (experimental group) and paraffin sealing in the right side (control group). Animals were euthanized at 7, 14 and 21 days after sealing the perforations. The expression levels of the IFN-γ, TNF-α, IL-10, IL-4, TGF-β and RANKL genes were investigated by real time polymerase chain reaction (PCR) in the teeth and surrounding tissues. In the experimental groups, after the 7th day, there was a down-regulation of the mRNA levels of TNF-α and IL-4 compared to the 14th day (p<0.05). In these groups, the mRNA levels of RANKL, IFN-γ and TNF-α were statistically higher after 14 days compared to 21 days post-MTA sealing (p<0.05). The level of IL-10 mRNA was increased at the 21st day (p<0.05). The mRNA expression of TGF-β did not exhibit any statistically relevant results. There was a statistical down-regulation of IL-4 gene expressions when control and experimental groups were compared at days 7 and 21. In conclusion, MTA sealing favored the expression of pro-inflammatory cytokines in the intermediate phase of the immuno-inflammatory response (14th day). The reduction of these cytokines in later phase of the response was probably due to immunoregulation by IL-10.
authors: Dental School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors: Viviane de Paula Lima
authors: Vde P
authors: Lara
authors: Dental School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors: Fabiano Pereira
authors: FP
authors: Cardoso
authors: Dental School, Universidade Itaúna, Itaúna, MG, BR.
authors: Luciana Carla Neves
authors: LC
authors: Brito
authors: Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors: Leda Quercia
authors: LQ
authors: Vieira
authors: Dental School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors: Antônio Paulino Ribeiro
authors: AP
authors: Sobrinho
authors: Post-graduation Program in Biotechnology and Genomic Sciences, Universidade Católica de Brasília, Brasília, DF, BR.
authors: Taia Maria Berto
authors: TM
authors: Rezende
mesh_terms: D017607:Aluminum Compounds; D000818:Animals; D017610:Calcium Compounds; D016207:Cytokines / Q000378:metabolism*; D004338:Drug Combinations; D051379:Mice; D008807:Mice, Inbred BALB C; D010087:Oxides; D060888:Real-Time Polymerase Chain Reaction; D017640:Silicates
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D016207:Cytokines; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate