title: Taxonomic description and genome sequence of Bacillus campisalis sp. nov., a member of the genus Bacillus isolated from a solar saltern.
abstract: The taxonomic position of a Gram-stain positive bacterium isolated from a solar saltern sample collected from Kanyakumari, coastal region of the Bay of Bengal, India, was analysed by using a polyphasic approach. The isolated strain, designated SA2-6T, had phenotypic characteristics that matched those of the genus Bacillus. The 16S rRNA gene sequence (1493 bases) of the novel strain was compared with those of previously studied Bacillus type strains and confirmed that the strain belongs to the genus Bacillus and was moderately closely related to the type strain of Bacillus foraminis at 97.5 % 16S rRNA gene sequence similarity, followed by those of Bacillus thioparans (96.9 %), Bacillus subterraneus (96.8 %), Bacillus jeotgali (96.6 %), Bacillus selenatarsenatis (96.6 %) and Bacillus boroniphilus (96.6 %). 16S rRNA gene sequence analysis indicated that strain SA2-6T differs from all other species of the genus Bacillus by at least 2.5 %. It contained MK-7 as the predominant menaquinone, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, and iso-C15 : 0 and anteiso-C15 : 0 as major fatty acids. Major lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on data from this polyphasic study, strain SA2-6T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus campisalis sp. nov. is proposed. The type strain is SA2-6T ( = MTCC 11848T = DSM 28801T). The draft genome of strain SA2-6T consisted of 5 183 363 bp with G+C content of 45.44 mol%, 5352 predicted coding sequences, 191 RNAs and 479 subsystems.
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Rajendran Mathan
authors: RM
authors: Kumar
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Gurwinder
authors: G
authors: Kaur
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Anand
authors: A
authors: Kumar
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Monu
authors: M
authors: Bala
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Nitin Kumar
authors: NK
authors: Singh
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Navjot
authors: N
authors: Kaur
authors: Division of Protein Science & Engineering, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Narender
authors: N
authors: Kumar
authors: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh, 160 036, India.
authors: Shanmugam
authors: S
authors: Mayilraj
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7