title: Maribacter confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring.
abstract: A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK2-2(T), was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK2-2(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain SSK2-2(T) fell within the clade comprising the type strains of species of the genus Maribacter, joining the type strain of Maribacter sedimenticola with which it shared 99.4% similarity. Sequence similarities to the type strains of other Maribacter species were 94.6-98.2%. Strain SSK2-2(T) contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0) as the major fatty acids. The major polar lipids of strain SSK2-2(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-2(T) was 38.2 mol% and mean levels of DNA-DNA relatedness with the type strains of four phylogenetically related species of the genus Maribacter were 11-24%. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK2-2(T) is separate from other Maribacter species. On the basis of the data presented, strain SSK2-2(T) is considered to represent a novel species of the genus Maribacter, for which the name Maribacter confluentis sp. nov. is proposed. The type strain is SSK2-2(T) ( = KCTC 42604(T) = CECT 8869(T)).
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors: Yong-Taek
authors: YT
authors: Jung
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Ji-Min
authors: JM
authors: Park
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sung-Min
authors: SM
authors: Won
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification; D005618:Fresh Water / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D009792:Oceans and Seas; D010714:Phosphatidylethanolamines; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; D012965:Sodium Chloride; C042455:menaquinone 6