title: GLT-1 Transport Stoichiometry Is Constant at Low and High Glutamate Concentrations when Chloride Is Substituted by Gluconate.
abstract: Glutamate is the major excitatory neurotransmitter, but prolonged exposure even at micromolar concentrations causes neuronal death. Extracellular glutamate is maintained at nanomolar level by glutamate transporters, which, however, may reverse transport and release glutamate. If and when the reverse occurs depends on glutamate transport stoichiometry (GTS). Previously we found that in the presence of chloride, the coupled GLT-1 glutamate transporter current and its relationship to radiolabeled glutamate flux significantly decreased when extracellular glutamate concentration increased above 0.2 mM, which implies a change in GTS. Such high concentrations are feasible near GLT-1 expressed close to synaptic release site during excitatory neurotransmission. The aim of this study was to determine GLT-1 GTS at both low (19-75 μM) and high (300-1200 μM) glutamate concentration ranges. GTS experiments were conducted in the absence of chloride to avoid contributions by the GLT-1 uncoupled chloride conductance. Mathematical analysis of the transporter thermodynamic equilibrium allowed us to derive equations revealing the number of a particular type of ion transported per elementary charge based on the measurements of the transporter reversal potential. We found that GLT-1a expressed in COS-7 cells co-transports 1.5 Na+, 0.5 Glu-, 0.5 H+ and counter-transports 0.6 K+ per elementary charge in both glutamate concentration ranges, and at both 37°C and 26°C temperatures. The thermodynamic parameter Q10 = 2.4 for GLT-1 turnover rate of 19 s-1 (37°C, -50 mV) remained constant in the 10 μM-10 mM glutamate concentration range. Importantly, the previously reported decrease in the current/flux ratio at high glutamate concentration was not seen in the absence of chloride in both COS-7 cells and cultured rat neurons. Therefore, only in the absence of chloride, GLT-1 GTS remains constant at all glutamate concentrations. Possible explanations for why apparent GTS might vary in the presence of chloride are discussed.
authors: F. M. Kirby Neurobiology Center and Department of Neurology, Children's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Anatoli Y
authors: AY
authors: Kabakov
authors: F. M. Kirby Neurobiology Center and Department of Neurology, Children's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Paul A
authors: PA
authors: Rosenberg
mesh_terms: D000818:Animals; D019556:COS Cells; D002712:Chlorides / Q000378:metabolism; D002522:Chlorocebus aethiops; D027342:Excitatory Amino Acid Transporter 2 / Q000096:biosynthesis / Q000235:genetics*; D005942:Gluconates / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D018408:Patch-Clamp Techniques; D051381:Rats; D012964:Sodium / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics*
chemical_list: D002712:Chlorides; D027342:Excitatory Amino Acid Transporter 2; D005942:Gluconates; D018698:Glutamic Acid; D012964:Sodium; C030691:gluconic acid