title: Interleukin-18, interleukin-12B and interferon-γ gene polymorphisms in Brazilian patients with rheumatoid arthritis: a pilot study.
abstract: Polymorphisms in interleukin (IL)-18, IL-12 and interferon (IFN)-γ genes are associated with different levels of cytokines expression and have been associated with rheumatoid arthritis (RA). IL-18 +105 A/C, IL-12B +1188 A/C and IFN-γ +874 T/A polymorphisms were analyzed by restriction fragment length polymorphism-polymerase chain reaction (PCR) and amplification refractory mutation system PCR from 90 RA patients and 186 healthy individuals. There were significant differences to IL-18 +105 A/C polymorphism between the RA and control groups (odds ratio = 3.77; P < 0.0001). Individual carriers of the variant allele C had a 3.77-fold increased risk of for RA (P = 0.0032). No association was observed for IL-12B and IFN-γ polymorphisms. Our finds suggest a possible role for IL-18 polymorphism in the RA susceptibility in studied population.
authors: Department of General Formation, Federal Institute of Pernambuco, Garanhuns, Pernambuco, Brazil.|Postgraduate Program in Genetics, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
authors: H D
authors: HD
authors: Angelo
authors: Postgraduate Program in Applied Molecular and Cellular Biology, University of Pernambuco (ICB/UPE), Recife, Pernambuco, Brazil.
authors: I I F
authors: II
authors: Gomes Silva
authors: Division of Clinical Immunology, Department of Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors: R D R
authors: RD
authors: Oliveira
authors: Division of Clinical Immunology, Department of Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors: P
authors: P
authors: Louzada-Júnior
authors: Division of Clinical Immunology, Department of Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors: E A
authors: EA
authors: Donadi
authors: Department of Genetics, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
authors: S
authors: S
authors: Crovella
authors: Department of Biology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
authors: M M D
authors: MM
authors: Maia
authors: Department of Biology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
authors: P R E
authors: PR
authors: de Souza
authors: Postgraduate Program in Genetics, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
authors: P
authors: P
authors: Sandrin-Garcia
mesh_terms: D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology; D053762:Interleukin-12 Subunit p40 / Q000235:genetics* / Q000276:immunology; D020382:Interleukin-18 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012306:Risk
chemical_list: C500879:IL12B protein, human; C000589760:IL18 protein, human; D053762:Interleukin-12 Subunit p40; D020382:Interleukin-18; D007371:Interferon-gamma
keywords: arthritis; cytokines; polymorphism; rheumatoid