title: Single tumor imaging with multiple antibodies targeting different antigens.
abstract: Antibodies are important drugs for treating cancer and there is strong rationale for using multiple antibodies to improve outcomes. We labeled two breast cancer binding antibodies, anti-ErbB2 and anti-EpCAM, with infrared fluorescence dyes of different wavelengths and determined their in vivo distribution in a breast cancer xenograft model using a near-infrared (NIR) fluorescence imaging system. Our data show that these two antibodies can be readily assessed simultaneously in mouse xenograft model. This will help guide design of dosing strategies for multiple antibodies and identify potential interaction that could affect pharmacokinetics and possible side effects.
authors: Department of Surgery, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA.
authors: Yujing
authors: Y
authors: Sun
authors: Department of Surgery, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA.
authors: Girja
authors: G
authors: Shukla
authors: Department of Surgery, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA.
authors: Stephanie C
authors: SC
authors: Pero
authors: Department of Pediatrics, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA.
authors: Erika
authors: E
authors: Currier
authors: Department of Pediatrics, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA.
authors: Giselle
authors: G
authors: Sholler
authors: Department of Surgery, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA.
authors: David
authors: D
authors: Krag
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000737:chemistry* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology*; D061848:Optical Imaging / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry / Q000276:immunology
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D005456:Fluorescent Dyes; D018719:Erb-b2 Receptor Tyrosine Kinases; C499379:EPCAM protein, human; C508053:ERBB2 protein, human
keywords: antibody; breast cancer; combination administration; mouse; near-infrared fluorescence imaging; xenograft