title: Down-regulation of MicroRNA-381 promotes cell proliferation and invasion in colon cancer through up-regulation of LRH-1.
abstract: The expression and roles of MicroRNA-381 (miR-381) has been explored in several types of human cancers. However, its biological functions in colon cancer remain largely unknown. Quantitative real-time PCR assays were used to detect the expression of miR-381 in human colon cancer tissues and adjacent normal tissues. miR-381 antisense oligos and mimics were introduced into SW480 and HCT116 cells. Bioinformatic prediction analysis was performed to identify the potential targets of miR-381. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-381. We found that miR-381 was significantly down-regulated in human colon cancer tissues, compared with adjacent normal tissues. Introduction of miR-381 antisense oligos into SW480 and HCT116 cells promoted cell proliferation and invasion. Besides, inhibition of miR-381 could also support tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the nuclear receptor liver receptor homologue 1 (LRH-1) is a target gene of miR-381. Thus, our data suggested that down-regulation of miR-381 plays an important role in the colon cancer progression.
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
authors: Yan
authors: Y
authors: Liang
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
authors: Qun
authors: Q
authors: Zhao
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
authors: Liqiao
authors: L
authors: Fan
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
authors: Zhidong
authors: Z
authors: Zhang
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
authors: Bibo
authors: B
authors: Tan
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
authors: Yu
authors: Y
authors: Liu
authors: The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China. Electronic address: li_yong_hbth@126.com.
authors: Yong
authors: Y
authors: Li
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019295:Computational Biology; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C588249:MIRN381 microRNA, human; D035683:MicroRNAs; C082631:NR5A2 protein, human; D016376:Oligonucleotides, Antisense; D018160:Receptors, Cytoplasmic and Nuclear
keywords: Colon cancer; LRH-1; MicroRNA; miR-381