title: Transcriptome-wide identification of Camellia sinensis WRKY transcription factors in response to temperature stress.
abstract: Tea plant [Camellia sinensis (L.) O. Kuntze] is a leaf-type healthy non-alcoholic beverage crop, which has been widely introduced worldwide. Tea is rich in various secondary metabolites, which are important for human health. However, varied climate and complex geography have posed challenges for tea plant survival. The WRKY gene family in plants is a large transcription factor family that is involved in biological processes related to stress defenses, development, and metabolite synthesis. Therefore, identification and analysis of WRKY family transcription factors in tea plant have a profound significance. In the present study, 50 putative C. sinensis WRKY proteins (CsWRKYs) with complete WRKY domain were identified and divided into three Groups (Group I-III) on the basis of phylogenetic analysis results. The distribution of WRKY family transcription factors among plantae, fungi, and protozoa showed that the number of WRKY genes increased in higher plant, whereas the number of these genes did not correspond to the evolutionary relationships of different species. Structural feature and annotation analysis results showed that CsWRKY proteins contained WRKYGQK/WRKYGKK domains and C2H2/C2HC-type zinc-finger structure: D-X18-R-X1-Y-X2-C-X4-7-C-X23-H motif; CsWRKY proteins may be associated with the biological processes of abiotic and biotic stresses, tissue development, and hormone and secondary metabolite biosynthesis. Temperature stresses suggested that the candidate CsWRKY genes were involved in responses to extreme temperatures. The current study established an extensive overview of the WRKY family transcription factors in tea plant. This study also provided a global survey of CsWRKY transcription factors and a foundation of future functional identification and molecular breeding.
authors: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Zhi-Jun
authors: ZJ
authors: Wu
authors: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Xing-Hui
authors: XH
authors: Li
authors: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Zhi-Wei
authors: ZW
authors: Liu
authors: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Hui
authors: H
authors: Li
authors: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Yong-Xin
authors: YX
authors: Wang
authors: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. zhuangjing@njau.edu.cn.
authors: Jing
authors: J
authors: Zhuang
mesh_terms: D028241:Camellia sinensis / Q000235:genetics*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Tea plant; Temperature stresses; Transcription factors; Transcriptome; WRKY