title: Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study.
abstract: Bacterial communities are known to play important roles during the developmental stages of insects, but current knowledge of bacteria associated with the midgut of Apis dorsata, the giant Asian honeybee, is limited. Using polymerase chain reaction-denaturing gradient gel electrophoresis analysis (PCR-DGGE) and 16S rRNA sequencing, the aim of this study was to determine the dynamics of bacterial community structure across four A. dorsata life stages in different geographical locations. The results reveal that bacterial diversity increased as the bee progressed through larval stage to newly emerged worker and old worker. However, in the pupal stage, no bands identified as bacteria could be observed. Overall, 2 bacterial phyla (Proteobacteria and Firmicutes) and 4 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Bacilli) were identified, but the frequency varied among the different stages and locations. The classes of Gammaproteobacteria and Bacilli dominated among larval, newly emerged worker and old worker developmental stages.
authors: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.|Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, NY, 10010, USA.
authors: Prakaimuk
authors: P
authors: Saraithong
authors: Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, NY, 10010, USA.
authors: Yihong
authors: Y
authors: Li
authors: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Kanokporn
authors: K
authors: Saenphet
authors: Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, NY, 10010, USA.
authors: Zhou
authors: Z
authors: Chen
authors: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.|Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Panuwan
authors: P
authors: Chantawannakul
mesh_terms: D000818:Animals; D001516:Bees / Q000254:growth & development / Q000382:microbiology*; D000068536:Bacillota / Q000235:genetics / Q000302:isolation & purification*; D041981:Gastrointestinal Tract / Q000382:microbiology; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013785:Thailand
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Apis dorsata; DGGE; gut flora; insect bacteria; microbial diversity