title: Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA.
abstract: In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and temperature were determined as 6 and 37 °C, respectively. Immobilized cells were three times more thermally stable compared to free cells at the optimum temperature and had a half-life (t 1/2) of 131 h. The free cells lost most of lysine decarboxylase activity after nine cycles, but in contrast immobilized cells retained 56% of their residual activity even after the 18th cycle. The immobilized cells gave a maximum production of cadaverine (75.8 g/L) with 84% conversion.
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Shashi Kant
authors: SK
authors: Bhatia
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Yong Hyun
authors: YH
authors: Kim
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Hyun Joong
authors: HJ
authors: Kim
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Hyung-Min
authors: HM
authors: Seo
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Jung-Ho
authors: JH
authors: Kim
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Hun-Seok
authors: HS
authors: Song
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Ganesan
authors: G
authors: Sathiyanarayanan
authors: Food Ingredients Center, Foods R&D, CheilJedang, Guro-dong, Guro-Gu, Seoul, 152-051, Republic of Korea.
authors: Sung-Hee
authors: SH
authors: Park
authors: Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong, 339-701, Republic of Korea.
authors: Kyungmoon
authors: K
authors: Park
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea. seokor@konkuk.ac.kr.|Microbial Carbohydrate Resource Bank, Konkuk University, Seoul, 143-701, Republic of Korea. seokor@konkuk.ac.kr.
authors: Yung-Hun
authors: YH
authors: Yang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D019149:Bioreactors; D001711:Biotransformation; D002103:Cadaverine / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000378:metabolism*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000378:metabolism*; D013696:Temperature
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine; D002103:Cadaverine
keywords: Barium alginate; Bioprocess; Cadaverine; Immobilization; Lysine