title: AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70.
abstract: This paper describes a novel, simple, and disposable immunosensor based on indium-tin oxide (ITO) sheets modified with gold nanoparticles to sensitively analyze heat shock protein 70 (HSP70), a potential biomarker that could be evaluated in diagnosis of some carcinomas. Disposable ITO coated Polyethylene terephthalate (PET) electrodes were used and modified with gold nanoparticles in order to construct the biosensors. Optimization and characterization steps were analyzed by electrochemical techniques such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Surface morphology of the biosensor was also identified by electrochemical methods, scanning electron microscopy (SEM), and atomic force microscopy (AFM). To interpret binding characterization of HSP70 to anti-HSP70 single frequency impedance method was successfully operated. Moreover, the proposed HSP70 immunosensor acquired good stability, repeatability, and reproducibility. Ultimately, proposed biosensor was introduced to real human serum samples to determine HSP70 sensitively and accurately.
authors: Namık Kemal University, School of Health, Nutrition and Dietetics Department, Tekirdağ, Turkey.
authors: Münteha Nur
authors: MN
authors: Sonuç Karaboğa
authors: Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
authors: Çiğdem Sayıklı
authors: ÇS
authors: Şimşek
authors: Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey. Electronic address: msezginturk@hotmail.com.
authors: Mustafa Kemal
authors: MK
authors: Sezgintürk
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D002277:Carcinoma / Q000097:blood* / Q000175:diagnosis; D058266:Dielectric Spectroscopy / Q000191:economics / Q000379:methods; D055088:Early Detection of Cancer / Q000191:economics / Q000379:methods*; D055664:Electrochemical Techniques / Q000191:economics / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D018840:HSP70 Heat-Shock Proteins; D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold
keywords: Gold nanoparticles; HSP70; Heat shock proteins; ITO; Immunosensor