title: Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization.
abstract: Determination of plant growth regulators (PGRs) in a signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. In this work, a microwave-assisted extraction-derivatization (MAED) method is developed for six acidic PGRs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. Accuracies are greatly improved, particularly for gibberellin A3 (-2.72 to -0.65%) as compared with those reported (-22 to -2%). Excellent selectivity and quite low detection limits (0.37-1.36 ng mL(-1)) are enabled by fluorescence detection-mass spectrum monitoring. Results show the significant differences in acidic PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-NAA), implying the PGRs induced variations of components and genes. This study provides, for the first time, an accurate and efficient determination for labile PGRs involved in STS and a promising concept for objectively evaluating the safety of transgenic foods.
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Mengge
authors: M
authors: Liu
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.|Northwest Institute of Plateau Biology, Chinese Academy of Sciences , Xining 810001, China.
authors: Guang
authors: G
authors: Chen
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Hailong
authors: H
authors: Guo
authors: Hubei University of Science and Technology , Xianning, 437100 China.
authors: Baolei
authors: B
authors: Fan
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Jianjun
authors: J
authors: Liu
authors: Qinghai Normal University , Xining, 810008 China.
authors: Qiang
authors: Q
authors: Fu
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Xiu
authors: X
authors: Li
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Xiaomin
authors: X
authors: Lu
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Xianen
authors: X
authors: Zhao
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Guoliang
authors: G
authors: Li
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Zhiwei
authors: Z
authors: Sun
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Lian
authors: L
authors: Xia
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Shuyun
authors: S
authors: Zhu
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Daoshan
authors: D
authors: Yang
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Ziping
authors: Z
authors: Cao
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.
authors: Hua
authors: H
authors: Wang
authors: Northwest Institute of Plateau Biology, Chinese Academy of Sciences , Xining 810001, China.
authors: Yourui
authors: Y
authors: Suo
authors: The Key Laboratory of Life-Organic Analysis, Qufu Normal University , Qufu 273165, Shandong, China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, Shandong, China.|Northwest Institute of Plateau Biology, Chinese Academy of Sciences , Xining 810001, China.
authors: Jinmao
authors: J
authors: You
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008872:Microwaves; D010937:Plant Growth Regulators / Q000032:analysis / Q000302:isolation & purification*; D010938:Plant Oils / Q000032:analysis*; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics
chemical_list: D010937:Plant Growth Regulators; D010938:Plant Oils
keywords: microwave-assisted extraction−derivatization (MAED); plant growth regulators (PGRs); signal transduction system (STS); transgenic food