title: A highly selective fluorescent probe for in vitro and in vivo detection of Hg(2+) .
abstract: In this paper, a simple fluorescent probe, rhodamine B derivatives (RS), was designed and prepared for sensitive detection of Hg(2+) in CH3CN/H2O (5/5, v/v). RS exhibits high selectivity and sensitivity toward Hg(2+) over other common metal ions, displaying a significant color change from colorless to pink in the presence of Hg(2+). The fluorescence responses remain stable over a broad pH range (5.0 to 9.0) and are suitable for detection under physiological conditions. Experimental results of HeLa cells and zebrafish show that RS is cell and organism permeable. We also demonstrate the acquisition of images of Hg(2+) in HeLa cells and zebrafish by using a simple fluorescence confocal imaging technique.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. qycai0001@hnu.edu.cn qycai0002@gmail.com.
authors: Quan
authors: Q
authors: Zhou
authors: Zeming
authors: Z
authors: Wu
authors: Xiaohua
authors: X
authors: Huang
authors: Fenfen
authors: F
authors: Zhong
authors: Qingyun
authors: Q
authors: Cai
mesh_terms: D000818:Animals; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008628:Mercury / Q000032:analysis* / Q000737:chemistry*; D061848:Optical Imaging; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D008628:Mercury; C029773:rhodamine B