title: Cultivation of oleaginous Rhodotorula mucilaginosa in airlift bioreactor by using seawater.
abstract: The enormous water resource consumption is a concern to the scale-up fermentation process, especially for those cheap fermentation commodities, such as microbial oils as the feedstock for biodiesel production. The direct cultivation of oleaginous Rhodotorula mucilaginosa in a 5-L airlift bioreactor using seawater instead of pure water led to a slightly lower biomass being achieved, at 17.2 compared to 18.1 g/L, respectively. Nevertheless, a higher lipid content of 65 ± 5% was measured in the batch using seawater as compared to the pure water batch. Both the salinity and osmotic pressure decreased as the cultivation time increased in the seawater batch, and these effects may contribute to the high tolerance for salinity. No effects were observed for the seawater on the fatty acid profiles. The major components for both batches using seawater and pure water were C16:0 (palmitic acid), C18:1 (oleic acid) and C18:2 (linoleic acid), which together accounted for over 85% of total lipids. The results of this study indicated that seawater could be a suitable option for scaling up the growth of oleaginous R. mucilaginosa, especially from the perspective of water resource utilization.
authors: Department of Chemical and Materials Engineering, Tunghai University, 181 Taiwan Harbor 3rd Rd., Taichung 407, Taiwan. Electronic address: hwyen@thu.edu.tw.
authors: Hong-Wei
authors: HW
authors: Yen
authors: Department of Chemical and Materials Engineering, Tunghai University, 181 Taiwan Harbor 3rd Rd., Taichung 407, Taiwan.
authors: Yu-Ting
authors: YT
authors: Liao
authors: Department of Chemical and Materials Engineering, Tunghai University, 181 Taiwan Harbor 3rd Rd., Taichung 407, Taiwan.
authors: Yi Xian
authors: YX
authors: Liu
mesh_terms: D056804:Biofuels / Q000600:supply & distribution; D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D019787:Linoleic Acid / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D019301:Oleic Acid / Q000032:analysis; D009997:Osmotic Pressure; D019308:Palmitic Acid / Q000032:analysis; D012248:Rhodotorula / Q000737:chemistry* / Q000254:growth & development*; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D013997:Time Factors; D014871:Water Microbiology
chemical_list: D056804:Biofuels; D008055:Lipids; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid
keywords: Airlift bioreactors; Microbial oils; Oleaginous; Salinity; Seawater