title: Inhibition of Yap2 activity by MAPKAP kinase Rck1 affects yeast tolerance to cadmium.
abstract: Yap2 is a cadmium responsive transcription factor that interacts with MAPK-activated protein (MAPKAP) kinase Rck1. We show that Rck1 deletion confers protection against cadmium toxicity and that the mechanism underlying this observation relies on Yap2. Rck1 removal from the yeast genome potentiates Yap2 activity by increasing protein half-life and delaying its nuclear export. As a consequence, several Yap2 antioxidant targets are over-activated by a mechanism that also requires Yap1. Several genes of the cell wall integrity (CWI) pathway are upregulated under cadmium stress in a Yap2 dependent way. We showed that deletion of CWI genes renders yeast cells more sensitive to cadmium. These findings led us to suggest that in response to cadmium stress Yap2 may serve a dual purpose: oxidative stress attenuation and cell wall maintenance.
authors: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, 2781-901 Oeiras, Portugal; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Avenida Athos da Silveira Ramos 149, Bloco A, Lab 547, Rio de Janeiro, RJ 21941-909, Brazil.
authors: Daiane
authors: D
authors: Mazzola
authors: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, 2781-901 Oeiras, Portugal.
authors: Catarina
authors: C
authors: Pimentel
authors: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, 2781-901 Oeiras, Portugal.
authors: Soraia
authors: S
authors: Caetano
authors: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, 2781-901 Oeiras, Portugal.
authors: Catarina
authors: C
authors: Amaral
authors: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, 2781-901 Oeiras, Portugal; Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal.
authors: Regina
authors: R
authors: Menezes
authors: Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal.
authors: Claudia N
authors: CN
authors: Santos
authors: Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Avenida Athos da Silveira Ramos 149, Bloco A, Lab 547, Rio de Janeiro, RJ 21941-909, Brazil. Electronic address: eliscael@iq.ufrj.br.
authors: Elis
authors: E
authors: Eleutherio
authors: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, 2781-901 Oeiras, Portugal. Electronic address: claudina@itqb.unl.pt.
authors: Claudina
authors: C
authors: Rodrigues-Pousada
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000975:Antioxidants / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D009154:Mutation; D018384:Oxidative Stress / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D000975:Antioxidants; C082904:CAD1 protein, S cerevisiae; D004785:Environmental Pollutants; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D002104:Cadmium; D017346:Protein Serine-Threonine Kinases; C407463:RCK1 protein, S cerevisiae
keywords: Cadmium; MAPK-activated protein; Rck1; Yap2; Yeast