title: Catalase eliminates reactive oxygen species and influences the intestinal microbiota of shrimp.
abstract: Intestinal innate immune response is an important defense mechanism of animals and humans against external pathogens. The mechanism of microbiota homeostasis in host intestines has been well studied in mammals and Drosophila. The reactive oxygen species (ROS) and antimicrobial peptides have been reported to play important roles in homeostasis. However, how to maintain the microbiota homeostasis in crustacean intestine needs to be elucidated. In this study, we identified a novel catalase (MjCAT) involved in ROS elimination in kuruma shrimp, Marsupenaeus japonicus. MjCAT mRNA was widely distributed in hemocytes, heart, hepatopancreas, gills, stomach, and intestine. After the shrimp were challenged with pathogenic bacteria via oral infection, the expression level of MjCAT was upregulated, and the enzyme activity was increased in the intestine. ROS level was also increased in the intestine at early time after oral infection and recovered rapidly. When MjCAT was knocked down by RNA interference (RNAi), high ROS level maintained longer time, and the number of bacteria number was declined in the shrimp intestinal lumen than those in the control group, but the survival rate of the MjCAT-RNAi shrimp was declined. Further study demonstrated that the intestinal villi protruded from epithelial lining of the intestinal wall were damaged by the high ROS level in MjCAT-knockdown shrimp. These results suggested that MjCAT participated in the intestinal host-microbe homeostasis by regulating ROS level.
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Hui-Ting
authors: HT
authors: Yang
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Ming-Chong
authors: MC
authors: Yang
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Jie-Jie
authors: JJ
authors: Sun
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Fang
authors: F
authors: Guo
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Jiang-Feng
authors: JF
authors: Lan
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Xian-Wei
authors: XW
authors: Wang
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
authors: Xiao-Fan
authors: XF
authors: Zhao
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China. Electronic address: jxwang@sdu.edu.cn.
authors: Jin-Xing
authors: JX
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000069196:Gastrointestinal Microbiome; D006706:Homeostasis; D007113:Immunity, Innate; D007422:Intestines / Q000276:immunology; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D060829:Arthropod Proteins; D017382:Reactive Oxygen Species; D002374:Catalase
keywords: Catalase; Intestinal innate immune response; Marsupenaeus japonicus; Pathogen population; Reactive oxygen species