title: The influence of impact delivery mode, lactation time, infant gender, maternal age and rural or urban life on total number of Lactobacillus in breast milk Isfahan - Iran.
abstract: BACKGROUND
  Breast milk is known as the most crucial postpartum issue in metabolic and immunologic programming of neonatal health. Human milk microbial changes over Lactation. The factors influencing the milk microbiome as well as potential impact of microbes on infant health have not yet been discovered. The objective was to identify pre- and post-natal factors that can potentially influence the bacterial communities inhabiting human milk.
  
  
  METHODS
  Breast milk samples (n = 40) with all full-term breastfed infants were collected from lactating randomized. Information on personal characteristics, dietary habits, information about infants were collected after birth. The samples were plated with serial dilutions on three selective culture media man rogosa sharp and then colonies were counted. Colonies tested for catalase reaction, Gram-staining and microscopic examination.
  
  
  RESULTS
  The result of this study showed that the overall incidence of positive Lactobacillus in mother's milk was 87.5%. The results based on (infant gender, mode of delivery, rural or urban and lactation time) rural or urban and lactation time were significant (P < 0.05). The results showed that all of the variables were significant in this regression model (P < 0.001). The median of log10 Lactobacillus counts in rural mothers, vaginal delivery, infant male gender and Lactation time for first 3-month were meaningfully high.
  
  
  CONCLUSIONS
  The findings of this study about the breast milk Lactobacillus potential probiotic bacteria of healthy Iranian mothers, suggested that the breast milk microbiome is significantly influenced by several factors, mode of delivery, rural or urban and lactation time.
authors: Department of Food Science and Technology, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Mansoureh
authors: M
authors: Taghizadeh
authors: Department of Food Science and Technology, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Maryam
authors: M
authors: Mirlohi
authors: Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Farkhondeh
authors: F
authors: Poursina
authors: Department of Food Science and Technology, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Golnoush
authors: G
authors: Madani
authors: Department of Biostatistics and Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Mehri
authors: M
authors: Khoshhali
authors: Department of Food Science and Technology, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Nimah
authors: N
authors: Bahreini
authors: Food Security Research Center, Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Hajieh Ghasemian
authors: HG
authors: Safaei
keywords: Breast milk; Lactobacillus; delivery; infant; lactation