title: Electrostatic dust collectors compared to inhalable samplers for measuring endotoxin concentrations in farm homes.
abstract: Paired electrostatic dust collectors (EDCs) and daily, inhalable button samplers (BS) were used concurrently to sample endotoxin in 10 farm homes during 7-day periods in summer and winter. Winter sampling included an optical particle counter (OPC) to measure PM2.5 and PM2.5-10 . Electrostatic dust collectors and BS filters were analyzed for endotoxin using the kinetic chromogenic Limulus amebocyte lysate assay. Optical particle counter particulate matter (PM) data were divided into two PM categories. In summer, geometric mean (geometric standard deviation) endotoxin concentrations were 0.82 EU/m(3) (2.7) measured with the BS and 737 EU/m(2) (1.9) measured with the EDC. Winter values were 0.52 EU/m(3) (3.1) for BS and 538 EU/m(2) (3.0) for EDCs. Seven-day endotoxin values of EDCs were highly correlated with the 7-day BS sampling averages (r = 0.70; P < 0.001). Analysis of variance indicated a 2.4-fold increase in EDC endotoxin concentrations for each unit increase of the ratio of PM2.5 to PM2.5-10 . There was also a significant correlation between BS and EDCs endotoxin concentrations for winter (r = 0.67; P < 0.05) and summer (r = 0.75; P < 0.05). Thus, EDCs sample comparable endotoxin concentrations to BS, making EDCs a feasible, easy to use alternative to BS for endotoxin sampling.
authors: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USA.
authors: B
authors: B
authors: Kilburg-Basnyat
authors: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USA.
authors: T M
authors: TM
authors: Peters
authors: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USA.
authors: S S
authors: SS
authors: Perry
authors: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USA. Peter-thorne@uiowa.edu.
authors: P S
authors: PS
authors: Thorne
mesh_terms: D016902:Air Pollution, Indoor / Q000032:analysis*; D004391:Dust / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D000072480:Farms; D006801:Humans; D012621:Seasons; D055672:Static Electricity
chemical_list: D004391:Dust; D004731:Endotoxins
keywords: Bioaerosol; Button sampler; Electrostatic dust collector; Lipopolysaccharide; Optical particle counter