title: Development of a Novel Chromogenic Medium for Improved Campylobacter Detection from Poultry Samples.
abstract: The presence of expanded-spectrum β-lactamase (ESBL)-producing Escherichia coli is a common problem in the isolation of Campylobacter from poultry samples using conventional cefoperazone-based selective media. A novel chromogenic medium (CM-HT), based on modified charcoal cefoperazone deoxycholate agar (mCCDA), has been developed as a solution for improved Campylobacter detection from poultry samples. Although the basic components of CM-HT are the same as mCCDA, CM-HT uses both granular charcoal and sodium cefoxitin to enhance viewability and inhibit ESBL-producing bacteria. All tested Campylobacter jejuni (n = 31) and Campylobacter coli (n = 6) strains grew and formed purple-colored colonies on CM-HT. In contrast, the growth of all other tested microorganisms, including ESBL-producing E. coli strains, was suppressed by this medium. Additionally, 84 poultry samples were examined for the presence of Campylobacter using the ISO 10272-1 method (enrichment with Bolton broth) and the NIHSJ-02 method (enrichment with Preston broth) with mCCDA and CM-HT media for the isolation. The numbers of samples from which Camplylobacter was detected on CM-HT using Preston and Bolton broth were 22 and 18, whereas the numbers on mCCDA were 22 and 13, respectively. Only Campylobacter was detected on CM-HT using both enrichment broths; however, there were 5 and 19 samples from which ESBL-producing E. coli was detected on mCCDA using Preston and Bolton broth, respectively. Thus, there was a significant difference between CM-HT and mCCDA in selectivity for ESBL-producing E. coli regardless of which enrichment broth was used. The results obtained demonstrated that CM-HT is a possible solution for the improved isolation of Campylobacter from poultry samples.
authors: Yokohama Research Center, JNC Corporation, 5-1, Ookawa, Kanazawa-ku, Yokohama, Kanagawa 236-8605, Japan; Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon Universitys 1866, Kameino, Fujisawa, Kanagawa 252-0880, Japan. h.teramura@jnc-corp.co.jp.
authors: Hajime
authors: H
authors: Teramura
authors: Yokohama Research Center, JNC Corporation, 5-1, Ookawa, Kanazawa-ku, Yokohama, Kanagawa 236-8605, Japan.
authors: Mihoko
authors: M
authors: Iwasaki
authors: Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon Universitys 1866, Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Hirokazu
authors: H
authors: Ogihara
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D017000:Campylobacter coli / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000302:isolation & purification; D002438:Cefoperazone / Q000378:metabolism; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D011200:Poultry / Q000382:microbiology*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D003470:Culture Media; D002438:Cefoperazone; D001618:beta-Lactamases