title: In vivo confocal Raman microspectroscopy of the human skin: highlighting of spectral markers associated to aging via a research of correlation between Raman and biometric mechanical measurements.
abstract: Skin plays a protective role against the loss of water and external aggression, including mechanical stresses. These crucial functions are ensured by different cutaneous layers, particularly the stratum corneum (SC). During aging, the human skin reveals some apparent modifications of functionalities such as a loss of elasticity. Our investigations aimed at demonstrating that Raman microspectroscopy, as a label-free technique with a high molecular specificity, is efficient to assess in vivo the molecular composition of the skin and the alterations underwent during aging. Our approach was based on a search for correlation between Raman data collected on healthy female volunteers of different ages (from 21 to 70 years old) by means of a remote confocal Raman and skin firmness measurements used as a reference method. Raman and biometric data were then submitted to a partial least square (PLS)-based data processing. Our experiments demonstrated the potential of Raman microspectroscopy to provide an objective in vivo assessment of the skin "biological age" that can be very different from the "chronological age" of the person. In addition, Raman features sensitive to the elasticity and the fatigability of the SC were highlighted. Thereafter, calibration transfer functions were constructed to show the possibility to compare the results obtained during two distinct measurement campaigns conducted with two Raman probes of the same conception. This approach could lead to several interesting prospects, in particular by objectifying the effects of dermocosmetic products on the superficial layers of the skin and by accessing some underlying molecular mechanisms.
authors: Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100, Reims, France.|CNRS UMR7369 MEDyC, SFR Cap-Santé, 51 rue Cognacq-Jay, 51096, Reims, France.
authors: Christophe
authors: C
authors: Eklouh-Molinier
authors: Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100, Reims, France.|CNRS UMR7369 MEDyC, SFR Cap-Santé, 51 rue Cognacq-Jay, 51096, Reims, France.
authors: Vincent
authors: V
authors: Gaydou
authors: Jobin Yvon SA, Raman Division, 59650, Villeneuve d'Ascq, France.
authors: Emmanuel
authors: E
authors: Froigneux
authors: Centre d'Etudes et de Recherche en Cosmétologie (CERCO), 75116, Paris, France.
authors: Pascale
authors: P
authors: Barlier
authors: Centre d'Etudes et de Recherche en Cosmétologie (CERCO), 75116, Paris, France.
authors: Virginie
authors: V
authors: Couturaud
authors: Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100, Reims, France.|CNRS UMR7369 MEDyC, SFR Cap-Santé, 51 rue Cognacq-Jay, 51096, Reims, France.
authors: Michel
authors: M
authors: Manfait
authors: Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100, Reims, France. olivier.piot@univ-reims.fr.|CNRS UMR7369 MEDyC, SFR Cap-Santé, 51 rue Cognacq-Jay, 51096, Reims, France. olivier.piot@univ-reims.fr.|Platform of Cellular and Tissular Imaging (PICT), Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096, Reims, France. olivier.piot@univ-reims.fr.
authors: Olivier
authors: O
authors: Piot
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging; D005260:Female; D006801:Humans; D008875:Middle Aged; D025341:Principal Component Analysis; D012867:Skin / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D055815:Young Adult
keywords: Biometric mechanical measurements; Calibration transfer functions; In vivo confocal Raman probe; Partial least square regression; Skin aging