title: Catalase-only nanoparticles prepared by shear alone: Characteristics, activity and stability evaluation.
abstract: Catalase is a promising therapeutic enzyme; however, it carries risks of inactivation and rapid degradation when it is used in practical bioprocess, such as delivery in vivo. To overcome the issue, we made catalase-only nanoparticles using shear stress alone at a moderate shear rate of 217s(-1) in a coaxial cylinder flow cell. Properties of nanoparticles, including particle size, polydispersity index and zeta potential, were characterized. The conformational changes of pre- and post-sheared catalase were determined using spectroscopy techniques. The results indicated that the conformational changes of catalase and reduction in α-helical content caused by shear alone were less significant than that by desolvation method. Catalase-only nanoparticles prepared by single shear retained over 90% of its initial activity when compared with the native catalase. Catalase nanoparticles lost only 20% of the activity when stored in phosphate buffer solution for 72h at 4°C, whereas native catalase lost 53% under the same condition. Especially, the activity of nanogranulated catalase was decreased only slightly in the simulated intestinal fluid containing α-chymotrypsin during 4h incubation at 37°C, implying that the catalase nanoparticle was more resistant to the degradation of proteases than native catalase molecules. Overall, catalase-only nanoparticles offered a great potential to stabilize enzymes for various pharmaceutical applications.
authors: Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Education Ministry Key Laboratory of Systems Bioengineering, Tianjin 300072, PR China.
authors: Xiao-Nan
authors: XN
authors: Huang
authors: Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Education Ministry Key Laboratory of Systems Bioengineering, Tianjin 300072, PR China.
authors: Xin-Ying
authors: XY
authors: Du
authors: Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Education Ministry Key Laboratory of Systems Bioengineering, Tianjin 300072, PR China.
authors: Jin-Feng
authors: JF
authors: Xing
authors: Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Education Ministry Key Laboratory of Systems Bioengineering, Tianjin 300072, PR China. Electronic address: gezhiq@tju.edu.cn.
authors: Zhi-Qiang
authors: ZQ
authors: Ge
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D004795:Enzyme Stability; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D033081:Shear Strength; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D002374:Catalase
keywords: Catalase; Conformation; Enzymatic activity; Nanoparticles; Shear stress; Stability