title: Thalassocola ureilytica gen. nov., sp. nov., of the family Phyllobacteriaceae isolated from seawater.
abstract: A Gram-stain-negative, aerobic, rod-shaped, non-flagellated marine bacterium, designated strain LS-861T, was isolated from seawater of the South China Sea (Taiwan). Strain LS-861T grew optimally at pH 7.0 and 30 °C in the presence of 3 % (w/v) NaCl. The novel strain shared highest 16S rRNA gene sequence similarity (91.5 % each) with 'Nitratireductor shengliensis' CGMCC 1.12519 and Hoeflea halophila JG120-1T and lower sequence similarity ( < 91.5 %) with other species. Phylogenetic analyses based on 16S rRNA gene sequences revealed a distinct taxonomic position attained by strain LS-861T within the clade that accommodated members of the family Phyllobacteriaceae. The major fatty acids were C16 : 0, iso-C17 : 1ω10c, C18 : 0 3-OH and C18 : 1ω7c/C18 : 1ω6c. The polar lipid profile was relatively simple as compared with other representatives of Phyllobacteriaceae, by having major amounts of diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid, and moderate amounts of three unidentified phospholipids and an unidentified aminolipid. The DNA G+C content was 61.2 mol%. The predominant quinone system was ubiquinone-10 (Q-10). The data in general and phylogenetic and polar lipid data in particular clearly distinguish the novel strain from related species at the genus level. Thus, strain LS-861T is suggested to represent a novel species of a new genus of the family Phyllobacteriaceae, for which the name Thalassocola ureilytica gen. nov., sp. nov. is proposed. The type strain of Thalassocola ureilytica is LS-861T ( = BCRC 80818T = JCM 30682T).
authors: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors: Shih-Yao
authors: SY
authors: Lin
authors: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors: Asif
authors: A
authors: Hameed
authors: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.|Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.
authors: Chiu-Chung
authors: CC
authors: Young
authors: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors: You-Cheng
authors: YC
authors: Liu
authors: Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors: Yi-Han
authors: YH
authors: Hsu
authors: Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan.
authors: Wei-Ching
authors: WC
authors: Chung
authors: Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan.
authors: Li-Sen
authors: LS
authors: Young
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2