title: Macro-optical trapping for sample confinement in light sheet microscopy.
abstract: Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.
authors: SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK.
authors: Zhengyi
authors: Z
authors: Yang
authors: SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK ; Institute of Physics, University of Tartu, Ravila 14c, Tartu, 50411, Estonia.
authors: Peeter
authors: P
authors: Piksarv
authors: The Scottish Oceans Institute, Gatty Marine Laboratory, School of Biology, University of St. Andrews, East Sands, St. Andrews, KY16 8LB, UK.
authors: David E K
authors: DE
authors: Ferrier
authors: School of Biology, Medical and Biological Sciences Building, University of St. Andrews, North Haugh, St. Andrews, KY16 9TF, UK.
authors: Frank J
authors: FJ
authors: Gunn-Moore
authors: SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK.
authors: Kishan
authors: K
authors: Dholakia
keywords: (110.0110) Imaging systems; (110.0180) Microscopy; (180.6900) Three-dimensional microscopy; (350.4855) Optical tweezers or optical manipulation