title: MicroRNA-134 modulates resistance to doxorubicin in human breast cancer cells by downregulating ABCC1.
abstract: OBJECTIVE
  To investigate the role of microRNAs in doxorubicin resistance of breast cancer cells.
  
  
  RESULTS
  MicroRNA-134 was down-regulated in doxorubicin-resistant and doxorubicin-sensitive breast cancer samples and MCF-7/ADR cell lines. After transfection of miR-134 mimics, an MTT assay confirmed that the MCF-7/ADR cell proliferation was inhibited. In the presence of doxorubicin, there was inhibition of cell proliferation. Transfection of miR-134 mimics induced MCF-7/ADR cell apoptosis. The expression of ABCC1 was significantly upregulated in doxorubicin-resistant or -sensitive breast cancer tissues and MCF-7/ADR cell lines. Over-expression of miR-134 decreased the expression of ABCC1 at the protein level.
  
  
  CONCLUSIONS
  MicroRNA-134 modulates resistance to doxorubicin in breast cancer cells by downregulating the expression of ABCC1 which is known to encode the multidrug resistance-associated protein 1.
authors: Department of Oncology, The Second Affiliated Hospital of Qingdao Medical College, No. 127 South Siliu Road, Qingdao, 266042, Shandong, People's Republic of China.
authors: Lin
authors: L
authors: Lu
authors: Department of Oncology, The Second Affiliated Hospital of Qingdao Medical College, No. 127 South Siliu Road, Qingdao, 266042, Shandong, People's Republic of China.
authors: Fang
authors: F
authors: Ju
authors: Department of Oncology, The Second Affiliated Hospital of Qingdao Medical College, No. 127 South Siliu Road, Qingdao, 266042, Shandong, People's Republic of China.
authors: Hui
authors: H
authors: Zhao
authors: Department of Oncology, The Second Affiliated Hospital of Qingdao Medical College, No. 127 South Siliu Road, Qingdao, 266042, Shandong, People's Republic of China. 18660229289@126.com.
authors: Xuezhen
authors: X
authors: Ma
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D001943:Breast Neoplasms / Q000503:physiopathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D004317:Doxorubicin / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm; D005562:Formazans / Q000032:analysis; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D008954:Models, Biological; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis*; D013778:Tetrazolium Salts / Q000032:analysis
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D005562:Formazans; D035683:MicroRNAs; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D013778:Tetrazolium Salts; C569273:MIRN134 microRNA, human; C079782:MTT formazan; C421109:multidrug resistance-associated protein 1
keywords: ABCC1; Breast cancer; Doxorubicin; Doxorubicin resistance; Drug resistance; MRP1; MicroRNA-134