title: Structural characterization of arginine-vasopressin and lysine-vasopressin by Fourier- transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation.
abstract: Arginine-vasopressin (AVP) and lysine-vasopressin (LVP) were analyzed by reversed-phase liquid chromatography/mass spectrometry (LC-MS) using Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) electrospray ionization (ESI) in the positive ion mode. LVP and AVP exhibited the protonated adduct [M+H](+) as the predominant ion at m/z 1056.43965 and at m/z 1084.44561, respectively. Infrared multiphoton dissociation (IRMPD), using a CO(2) laser source at a wavelength of 10.6 μm, was applied to protonated vasopressin molecules. The IRMPD mass spectra presented abundant mass fragments essential for a complete structural information. Several fragment ions, shared between two target molecules, are discussed in detail. Some previously unpublished fragments were identified unambiguously utilizing the high resolution and accurate mass information provided by the FT-ICR mass spectrometer. The opening of the disulfide loop and the cleavage of the peptide bonds within the ring were observed even under low-energy fragmentation conditions. Coupling the high-performance FT-ICR mass spectrometer with IRMPD as a contemporary fragmentation technique proved to be very promising for the structural characterization of vasopressin.
authors: Dipartimento di Scienze, Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell'Ateneo Lucano, 10; 85100 Potenza, Italy. giuliana.bianco@unibas.it.
authors: Giuliana
authors: G
authors: Bianco
authors: Dipartimento di Scienze, Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell'Ateneo Lucano, 10; 85100 Potenza, Italy. fabiogiuseppe.battista@gmail.com.
authors: Fabio
authors: F
authors: Battista
authors: Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell'Ateneo Lucano, 10; 85100 Potenza, Italy. alessandro.buchicchio@unibas.it.
authors: Alessandro
authors: A
authors: Buchicchio
authors: Dipartimento di Scienze, Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell'Ateneo Lucano, 10; 85100 Potenza, Italy. amarenafasano@tiscali.it.
authors: Concetta G
authors: CG
authors: Amarena
authors: German Research Center for Environmental Health, Department of BioGeoChemistry and Analytics, Helmholtz Zentrum Munchen; D-85764 Neuherberg, Germany. schmitt-kopplin@helmholtz-muenchen.de.
authors: Philippe
authors: P
authors: Schmitt-Kopplin
authors: Dipartimento di Scienze, Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell'Ateneo Lucano, 10; 85100 Potenza, Italy. antonio.guerrieri@unibas.it.
authors: Antonio
authors: A
authors: Guerrieri
mesh_terms: D001127:Arginine Vasopressin / Q000737:chemistry*; D017357:Cyclotrons; D008236:Lypressin / Q000032:analysis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D001127:Arginine Vasopressin; D008236:Lypressin