title: A novel bioactive membrane by cell electrospinning.
abstract: Electrospinning permits fabrication of biodegradable matrices that can resemble the both scale and mechanical behavior of the native extracellular matrix. However, achieving high-cellular density and infiltration of cells within matrices with traditional technique remain challenging and time consuming. The cell electrospinning technique presented in this paper can mitigate the problems associated with these limitations. Cells encapsulated by the material in the cell electrospinning technique survived well and distributed homogenously within the nanofibrous membrane, and their vitality was improved to 133% after being cultured for 28 days. The electrospun nanofibrous membrane has a certain degradation property and favorable cell-membrane interaction that supports the active biocompatibility of the membrane. Its properties are helpful for supporting cell attachment and growth, maintaining phenotypic shape, and secreting an ample amount of extracellular matrix (ECM). This novel membrane may be a potential application within the field of tissue engineering. The ability of cell electrospinning to microintegrate cells into a biodegradable fibrous matrix embodies a novel tissue engineering approach that could be applied to fabricate a high cell density elastic tissue mimetic.
authors: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China; School of Mechanical and Electrical Engineering, Jinggangshan University, Ji'an 343009, China.
authors: Haiping
authors: H
authors: Chen
authors: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China; Shanghai Key Laboratory of Intelligent Manufacturing and Roboties, Shanghai 200444, China. Electronic address: Yuanyuan_liu@shu.edu.cn.
authors: Yuanyuan
authors: Y
authors: Liu
authors: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China; Shanghai Key Laboratory of Intelligent Manufacturing and Roboties, Shanghai 200444, China.
authors: Qingxi
authors: Q
authors: Hu
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008566:Membranes / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D057139:Nanofibers; D036103:Nanotechnology / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials
keywords: Bioactive membrane; Cell compatibility; Cell electrospinning; Cell viability