title: Attenuated Airway Epithelial Cell Interleukin-22R1 Expression in the Infant Nonhuman Primate Lung.
abstract: Respiratory tract infections are a leading cause of morbidity and mortality in children under 5 years of age. Increased susceptibility to infection is associated with deficiencies in immunity during early childhood. Airway epithelium represents the first line of mucosal defense against inhaled pathogens. However, little is known about epithelial immune mechanisms in the maturing lung. IL-22 and its receptor IL-22R1 are important in host defense and repair of epithelial barriers. The objective of this study was to determine whether a quantitative difference in IL-22R1 exists between infant and adult airways using the rhesus macaque monkey as a model of childhood lung development. Immunofluorescence staining of tracheal tissue revealed minimal expression of IL-22R1 in epithelium at 1 month of age, with a progressive increase in fluorescence-positive basal cells through 1 year of age. Western blot analysis of tracheal lysates confirmed significant age-dependent differences in IL-22R1 protein content. Further, primary tracheobronchial epithelial cell cultures established from infant and adult monkeys showed differential IL-22R1 mRNA and protein expression in vitro. To begin to assess the regulation of age-dependent IL-22R1 expression in airway epithelium, the effect of histone deacetylase and DNA methyltransferase inhibitors was evaluated. IL-22R1 mRNA in adult cultures was not altered by 5-aza-2'-deoxycytidine or trichostatin A. IL-22R1 mRNA in infant cultures showed no change with 5-aza-2'-deoxycytidine but was significantly increased after trichostatin A treatment; however, IL-22R1 protein did not increase concurrently. These data suggest that IL-22R1 in airway epithelium is regulated, in part, by epigenetic mechanisms that are dependent on chronologic age.
authors: 1 California National Primate Research Center, University of California, Davis, California; and.
authors: Daniel T
authors: DT
authors: Dugger
authors: 1 California National Primate Research Center, University of California, Davis, California; and.
authors: Joan E
authors: JE
authors: Gerriets
authors: 1 California National Primate Research Center, University of California, Davis, California; and.|2 Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California.
authors: Lisa A
authors: LA
authors: Miller
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D044127:Epigenesis, Genetic; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008168:Lung / Q000254:growth & development / Q000378:metabolism*; D008253:Macaca mulatta; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism*; D053701:Receptors, Interleukin-10 / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; C415369:interleukin-22 receptor
keywords: epigenetic; innate immunity; primate; respiratory development; trichostatin A