title: Field-induced wooden-tip electrospray ionization mass spectrometry for high-throughput analysis of herbal medicines.
abstract: This study demonstrates the first application of field-induced wooden-tip electrospray ionization (ESI) mass spectrometry (MS) for high-throughput analysis of herbal medicines. By application of an opposite and sample-contactless high voltage on the MS inlet rather than wooden tips, a high-throughput analysis device is easily set up, and a relatively fast analysis speed of 6 s per sample was successfully achieved. In addition, fast polarity switching between positive and negative ion detection mode is readily accomplished, which provides more complete chemical information for quality assessment and control of herbal medicines. By using the proposed method, various active ingredients present in different herbal medicines were rapidly detected, and the obtained mass spectra were served as the samples' fingerprints for tracing the origins, establishing the authenticity, and assessing the quality consistency and stability of herbal medicines. Our experimental results demonstrated that field-induced wooden-tip ESI-MS is a desirable method for high-throughput analysis of herbal medicines, with promising prospects for rapidly differentiating the origin, determining the authenticity, and assessing the overall quality of pharmaceuticals.
authors: Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals and Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center Guangzhou, Guangzhou, 510070, China. Electronic address: yy_yang@vip.126.com.
authors: Yunyun
authors: Y
authors: Yang
authors: Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals and Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center Guangzhou, Guangzhou, 510070, China; Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen, 518057, China.
authors: Jiewei
authors: J
authors: Deng
authors: Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen, 518057, China. Electronic address: zhongping.yao@polyu.edu.hk.
authors: Zhong-Ping
authors: ZP
authors: Yao
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
chemical_list: D004365:Drugs, Chinese Herbal; C508895:yin-huang
keywords: Field-induced; Herbal medicines; High-throughput analysis; Mass spectrometry; Wooden-tip electrospray ionization