title: 5-HT Receptor Antagonism Attenuates the Ischemia-Reperfusion Injury After Rabbit Lung Preservation.
abstract: The success of lung transplantation is threatened by the appearance of ischemia-reperfusion injury, which is characterized by increased vascular permeability. 5-Hydroxytryptamine (5-HT; serotonin) is known to produce microvascular leakage in the systemic circulation, but its possible role in ischemia-reperfusion injury after lung preservation has not been reported. In this work we measured the release of 5-HT during a 24-hour rabbit lung preservation, and the effect of methiothepin (antagonist of the majority of 5-HT receptors) and SB204741 (antagonist of 5-HT2B/2C receptors) on the modified capillary filtration coefficient (mKf,c) was evaluated at the end of this period. Our results showed that the highest release rate of 5-HT occurred during the first 15 minutes after the lung harvesting and progressively decreased in the following time intervals. The baseline mKf,c greatly increased after 24 hours of lung preservation, and this increment was partially reduced by methiothepin and even more by SB204741. We concluded that 5-HT may play an important role in the ischemia-reperfusion process after lung preservation.
authors: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico. Electronic address: arreolaj2002@yahoo.com.mx.
authors: J L
authors: JL
authors: Arreola-Ramírez
authors: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico.
authors: J
authors: J
authors: Alquicira-Mireles
authors: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico.
authors: P E
authors: PE
authors: Morales-Hernández
authors: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico.
authors: M H
authors: MH
authors: Vargas
authors: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico.
authors: J
authors: J
authors: Villalba-Caloca
authors: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico.
authors: P
authors: P
authors: Segura-Medina
mesh_terms: D000818:Animals; D002196:Capillaries; D007511:Ischemia / Q000378:metabolism*; D008168:Lung / Q000098:blood supply / Q000378:metabolism*; D055370:Lung Injury / Q000378:metabolism*; D016040:Lung Transplantation; D008297:Male; D009926:Organ Preservation / Q000009:adverse effects*; D011817:Rabbits; D015424:Reperfusion; D015427:Reperfusion Injury / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000378:metabolism*
chemical_list: D012702:Serotonin Antagonists