title: Smoke, pheromone and kairomone olfactory receptor neurons in males and females of the pine sawyer Monochamus galloprovincialis (Olivier) (Coleoptera: Cerambycidae).
abstract: The response of antennal olfactory receptor neurons (ORNs) of Monochamus galloprovincialis to several odourants was tested using single sensillum electrophysiology. Behaviourally active pheromone, and kairomone (host and sympatric bark beetle pheromone) odours were tested alongside smoke compounds released by burnt wood that are potentially attractive to the insect. The antennae bore several types of sensilla. Two plate areas in the proximal and distal ends of each antennal segment were covered with basiconic sensilla that responded to the odour stimuli. Sensilla basiconica contained one or two cells of different spike amplitude. The 32 male and 38 female ORNs tested responded with excitations or inhibitions to the different plant odours. In general the response of male and female receptors was very similar so they were pooled to perform a cluster analysis on ORN responses. Six ORNs were clearly specialised for pheromone reception. Responses to kairomone and smoke odours were less specific than those of pheromone, but a group of 9 cells was clearly excited by smoke compounds (mainly eugenol and 4-methyl 2-methoxyphenol), a group of 8 cells was very responsive to α-pinene, β-pinene and cis-verbenol, and a group of 14 cells responded to a wider range of compounds. The rest of the cells (47%) were either non-responsive or slightly inhibited by smoke compounds. Dose-response curves were obtained for several compounds. Different compounds induced significantly different latencies and these appeared to be unrelated to their boiling point.
authors: Sustainable Forest Management Research Institute, University of Valladolid-CIFOR-INIA, Av. Madrid 44, 34004 Palencia, Spain. Electronic address: gonzalbaz@gmail.com.
authors: Gonzalo
authors: G
authors: Álvarez
authors: Department of Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: Byrappa
authors: B
authors: Ammagarahalli
authors: Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK.
authors: David R
authors: DR
authors: Hall
authors: Sustainable Forest Management Research Institute, University of Valladolid-CIFOR-INIA, Av. Madrid 44, 34004 Palencia, Spain.
authors: Juan A
authors: JA
authors: Pajares
authors: Department of Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: César
authors: C
authors: Gemeno
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000648:ultrastructure; D001517:Coleoptera / Q000187:drug effects / Q000502:physiology*; D055724:Electrophysiological Phenomena; D005260:Female; D008297:Male; D039821:Monoterpenes / Q000494:pharmacology; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000187:drug effects* / Q000502:physiology; D010675:Pheromones / Q000494:pharmacology*; D028223:Pinus / Q000737:chemistry; D058618:Sensilla / Q000187:drug effects; D012906:Smoke
chemical_list: D039821:Monoterpenes; D010675:Pheromones; D012906:Smoke
keywords: Attractant; Detector; Electrophysiology; Olfaction; Pine wilt disease; Single sensillum recording