title: Decontamination of poultry feed from ochratoxin A by UV and sunlight radiations.
abstract: BACKGROUND
  Mycotoxin-contaminated feed is very dangerous for the growth and even life of poultry. The objective of the current study was to investigate the efficacy of ultra-violet irradiation for decontamination of ochratoxin A (OTA) in spiked and naturally contaminated poultry feed samples. Spiked and naturally contaminated feed samples were irradiated with ultra-violet light (UV) at distance of 25 cm over the feed samples. In vitro, the effect of UV intensity (0.1 mW cm(-2) at 254 nm UV-C) on different types of poultry feeds contaminated with OTA was evaluated. The same samples were also irradiated with sunlight and analysed for OTA by an indirect enzyme linked immunosorbent assay method.
  
  
  RESULTS
  Poultry feed samples containing 500 µg kg(-1) were 100% decontaminated in 180 min with UV radiation while OTA was decreased to 70-95 µg kg(-1) using the same poultry feed samples after 8 h sunlight irradiation. Therefore, UV light was found to be more effective. Only 1 h of UV irradiation was found to be sufficient to bring the OTA level to the maximum regulatory limit suggested for poultry feeds (100 µg kg(-1) ), while 8 h were needed to obtain this level using sunlight radiations.
  
  
  CONCLUSIONS
  The proposed approach is a viable option to reduce the level of OTA in contaminated poultry feeds. © 2015 Society of Chemical Industry.
authors: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors: Gul
authors: G
authors: Ameer Sumbal
authors: Dr M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors: Zahid
authors: Z
authors: Hussain Shar
authors: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors: Syed Tufail
authors: ST
authors: Hussain Sherazi
authors: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors: Sirajuddin
authors: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors: Shafi Muhammad
authors: SM
authors: Nizamani
authors: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
authors: Safaraz Ahmed
authors: SA
authors: Mahesar
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D005506:Food Contamination; D059022:Food Safety; D009793:Ochratoxins / Q000737:chemistry* / Q000528:radiation effects*; D011200:Poultry; D013472:Sunlight; D014466:Ultraviolet Rays
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
keywords: decontamination; ochratoxin A; poultry feed; sunlight; ultra-violet radiation