title: Anti-inflammatory and analgesic activities with gastroprotective effect of semi-purified fractions and isolation of pure compounds from Mediterranean gorgonian Eunicella singularis.
abstract: OBJECTIVE
  To explore anti-inflammatory activities of organic extract and its semi-purified fractions (ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis.
  
  
  METHODS
  The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature.
  
  
  RESULTS
  The fraction F-EtOH showed an important anti-inflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols.
  
  
  CONCLUSIONS
  These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.
authors: Laboratoire de développement chimique, galénique et pharmacologique des médicaments (LR12ES09). Equipe de Pharmacologie marine, Faculté de pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisia. Electronic address: moniadeghrigue@yahoo.fr.
authors: Monia
authors: M
authors: Deghrigue
authors: Department of Pharmacy, University of Naples "Federico II", via D. Montesano 49, I- 80131 Napoli, Italy.
authors: Carmen
authors: C
authors: Festa
authors: Laboratoire de chimie hétérocyclique, produits naturels et réactivité. Equipe de chimie médicinale et produits naturels (LR11ES39), Faculté des sciences de Monastir, Université de Monastir, 5000 Monastir, Tunisia.
authors: Lotfi
authors: L
authors: Ghribi
authors: Department of Pharmacy, University of Naples "Federico II", via D. Montesano 49, I- 80131 Napoli, Italy.
authors: Maria Valeria
authors: MV
authors: D'Auria
authors: Department of Pharmacy, University of Naples "Federico II", via D. Montesano 49, I- 80131 Napoli, Italy.
authors: Simona
authors: S
authors: De Marino
authors: Laboratoire de chimie hétérocyclique, produits naturels et réactivité. Equipe de chimie médicinale et produits naturels (LR11ES39), Faculté des sciences de Monastir, Université de Monastir, 5000 Monastir, Tunisia.
authors: Hichem
authors: H
authors: Ben Jannet
authors: Laboratoire de développement chimique, galénique et pharmacologique des médicaments (LR12ES09). Equipe de Pharmacologie marine, Faculté de pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisia.
authors: Abderrahman
authors: A
authors: Bouraoui
keywords: Analgesic activity; Anti-inflammatory activity; Diterpenoid; Eunicella singularis; Gastroprotective effect; Sterols