title: Verification by the FISH translocation assay of historic doses to Mayak workers from external gamma radiation.
abstract: The aim of this study was to apply the fluorescence in situ hybridization (FISH) translocation assay in combination with chromosome painting of peripheral blood lymphocytes for retrospective biological dosimetry of Mayak nuclear power plant workers exposed chronically to external gamma radiation. These data were compared with physical dose estimates based on monitoring with badge dosimeters throughout each person's working life. Chromosome translocation yields for 94 workers of the Mayak production association were measured in three laboratories: Southern Urals Biophysics Institute, Leiden University Medical Center and the former Health Protection Agency of the UK (hereinafter Public Health England). The results of the study demonstrated that the FISH-based translocation assay in workers with prolonged (chronic) occupational gamma-ray exposure was a reliable biological dosimeter even many years after radiation exposure. Cytogenetic estimates of red bone marrow doses from external gamma rays were reasonably consistent with dose measurements based on film badge readings successfully validated in dosimetry system "Doses-2005" by FISH, within the bounds of the associated uncertainties.
authors: Southern Urals Biophysics Institute (SUBI), 19 Ozyorskoe Shosse, Ozyorsk, Chelyabinsk Region, 456780, Russian Federation. sotnik@subi.su.
authors: Natalia V
authors: NV
authors: Sotnik
authors: Southern Urals Biophysics Institute (SUBI), 19 Ozyorskoe Shosse, Ozyorsk, Chelyabinsk Region, 456780, Russian Federation.
authors: Tamara V
authors: TV
authors: Azizova
authors: Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands.|Department of Health Science, Centre for Human Safety and Environmental Research, College of North Atlantic, Doha, Qatar.
authors: Firouz
authors: F
authors: Darroudi
authors: Public Health England, Chilton, Oxfordshire, UK.
authors: Elizabeth A
authors: EA
authors: Ainsbury
authors: Public Health England, Chilton, Oxfordshire, UK.
authors: Jayne E
authors: JE
authors: Moquet
authors: Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands.
authors: Janna
authors: J
authors: Fomina
authors: Public Health England, Chilton, Oxfordshire, UK.
authors: David C
authors: DC
authors: Lloyd
authors: Public Health England, Chilton, Oxfordshire, UK.
authors: Pat A
authors: PA
authors: Hone
authors: Public Health England, Chilton, Oxfordshire, UK.
authors: Alan A
authors: AA
authors: Edwards
mesh_terms: D065638:Absorption, Radiation; D000368:Aged; D001681:Biological Assay / Q000379:methods*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020223:Chromosome Painting; D005260:Female; D005720:Gamma Rays; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000502:physiology* / Q000528:radiation effects; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D000069079:Radiation Exposure / Q000032:analysis*; D018788:Radioactive Hazard Release; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic / Q000528:radiation effects; D014915:Whole-Body Counting
keywords: Badge doses; Dose validation; External gamma rays; FISH; Translocations