title: [Effect of PKF118-310 on Cell Cycle and Proliferation of K562 Cells and Its Mechanism].
abstract: Objetive: To investigate the effects of PKF118-310 on cell cycle and proliferation of K562 cell lines and its mechanism.
  
  
  METHODS
  After treatment of PKF118-310 with different concentration, the proliferation inhibition on K562 cell lines was detected by MTT, the existance of β-catenin and TCF-4 in the cells was observed by immunohistochemistry. The change of the cell cycle was detected by flow cytometry. The expressions of caspase-3, β-catenin, TCF and BCL-9 were detected by Western blot.
  
  
  RESULTS
  PKF118-310 can inhibit the proliferation of K562 cell line by S phase blocking. The β-catenin and TCF in the cells were observed by immunohistochemistry. After treating this cell line with PKF118-310 of different concentrations for 72 h, the expression level of caspase-3 increased, the expression levels of β-catenin, TCF and BCL-9 significantly decreased.
  
  
  CONCLUSIONS
  PKF118-310 induces cycle arest of K562 cells at the S phase and inhibits the proliferation of these cells through decreasing β-catenin/TCF/BCL-9 thrascriptional activity.
authors: Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China.
authors: Dan-Yu
authors: DY
authors: Wang
authors: Department of Hematology, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Scienes and Techonolgy, Wuhan 430022, Hubei Province, China.
authors: Wei-Ming
authors: WM
authors: Li
authors: Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China.
authors: Meng-Di
authors: MD
authors: Jing
authors: Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China.
authors: Hai-Yan
authors: HY
authors: Cui
authors: Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China.
authors: Bo
authors: B
authors: Lu
authors: Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China.
authors: Xin
authors: X
authors: Wang
authors: Department of Hematology, Nanshan Hospital Affiliated to Guangdong Medical College, Shenzhen 518052, Guangdong Province, China. E-mail: liuzelin1962@163.com.
authors: Ze-Lin
authors: ZL
authors: Liu
mesh_terms: D053148:Caspase 3; D002453:Cell Cycle; D049109:Cell Proliferation; D006801:Humans; D020014:K562 Cells; D011744:Pyrimidinones; D014227:Triazines; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; C548732:PKF118-310; D011744:Pyrimidinones; D014227:Triazines; D051176:beta Catenin; C505407:CASP3 protein, human; D053148:Caspase 3