title: The inhibitory effects of AR/miR-190a/YB-1 negative feedback loop on prostate cancer and underlying mechanism.
abstract: Prostate cancer at advanced stages including metastatic and castration-resistant cancer remains incurable due to the lack of effective therapies. MiR-190a belongs to the small noncoding RNA family and has an important role in breast cancer metastasis. However, it is still unknown whether miR-190a plays a role in prostate cancer development. Herein, we first observed AR/miR-190a/YB-1 forms an auto-regulatory negative feedback loop in prostate cancer: miR-190a expression was down-regulated by AR activation; YB-1 functions are as an AR activator; miR-190a inhibited AR expression and transactivation through direct binding to 3'UTR of YB-1 gene. MiR-190a contributes the human prostate cancer cell growth through AR-dependent signaling. Moreover, we examined the expression of miR-190a and observed a significant decrease in human prostate cancers. Reduced expression of miR-190a was inversely correlated to AR levels of prostate cancer patients, and patients with higher miR-190a expression in their tumor have improved tumor-free survival. Taken together, our findings identified a biochemical and functional link between miR-190a with reduced expression in advanced prostate cancer, YB-1 and AR signaling in prostate cancer.
authors: Department of Gynecology, Shanghai First Matenity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Shaohua
authors: S
authors: Xu
authors: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Tao
authors: T
authors: Wang
authors: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Wen
authors: W
authors: Song
authors: Department of General Surgery, QingZhou People Hospital, QinZhou, China.
authors: Tao
authors: T
authors: Jiang
authors: Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, China.
authors: Feng
authors: F
authors: Zhang
authors: Department of Pathology, Anhui Medical University, Hefei, China.
authors: Yu
authors: Y
authors: Yin
authors: Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA, USA.
authors: Shi-Wen
authors: SW
authors: Jiang
authors: Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Kongming
authors: K
authors: Wu
authors: Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Zuoren
authors: Z
authors: Yu
authors: Key Laboratory of Tianjin Radiation and Molecular Nuclear Medicine; Institute of Radiation Medicine, Peking Union Medical College &Chinese Academy of Medical Sciences, Tianjin, China.
authors: Chenguang
authors: C
authors: Wang
authors: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Ke
authors: K
authors: Chen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000728:Androgens / Q000494:pharmacology; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D025461:Feedback, Physiological / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011485:Protein Binding / Q000187:drug effects; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D051840:Y-Box-Binding Protein 1 / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C410798:AR protein, human; D000728:Androgens; C576613:MIRN190 microRNA, human; D035683:MicroRNAs; D011944:Receptors, Androgen; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human