title: Inhibitory effects of preimplantation exposure to bisphenol-A on blastocyst development and implantation.
abstract: The effect of preimplantation exposure to bisphenol-A (BPA) on blastocyst development and implantation is investigated. Mice were orally administered with BPA (200, 400, 600, and 800 mg/kg/day) from Day 0.5 to Day 3.5 of their pregnancy. Blastocyst development was examined on Day 4 of pregnancy. With 400 mg/kg/day BPA, implantation site number and implantation rate significantly reduced. With 600 and 800 mg/kg/day BPA, no implantation site was observed. BPA at 800 mg/kg/day significantly reduced blastocyst development rate and hatching rate. With 400 and 600 mg/kg/day BPA, Blastocyst development rate showed no significant difference whereas hatching rate was lower. With 400, 600, and 800 mg/kg/day BPA, some embryos were detected in the fallopian tube and hatched blastocysts showed greatly increased apoptosis level and endothelial nitric oxide synthase expression. In summary, high concentration BPA delayed the transfer of embryos to the uterus, damaged blastocyst development before implantation, and inhibited embryo implantation.
authors: Department of Histology and Embryology, Jilin Medical College Jilin, China.
authors: Xiaoyan
authors: X
authors: Pan
authors: Reproductive Medicine Center of The Affiliated Hospital of Jining Medical College Jining, China.
authors: Xuenan
authors: X
authors: Wang
authors: Department of Histology and Embryology, Jilin Medical College Jilin, China.
authors: Yanmei
authors: Y
authors: Sun
authors: Department of Histology and Embryology, Jilin Medical College Jilin, China.
authors: Zhaohua
authors: Z
authors: Dou
authors: Department of Histology and Embryology, Jilin Medical College Jilin, China.
authors: Zhixin
authors: Z
authors: Li
keywords: Bisphenol A; apoptosis; blastocyst development; embryo implantation; endothelial nitric oxide synthase