title: The localization and differential expression of Serum Amyloid A in bovine liver and adipose tissue depots.
abstract: In this article the localization of the acute phase protein Serum Amyloid A (SAA) in different depots of bovine adipose tissue (AT) and liver is reported. Quantitative (Real Time) PCR was paired to immunohistochemistry after the production of a specific polyclonal antibody. SAA's mRNA was found in all analyzed AT depots included in the present study, the AT located in the withers being the major source of SAA mRNA. A polyclonal antibody was raised against bovine SAA and was used to validate gene expression analyses. Western Blotting confirmed that SAA is present in all the seven adipose tissue depots include in the present experiment. Anti-SAA polyclonal antibody also stained diffusely adipocytes. In liver, intracytoplasmic immunolabeling was observed in hepatocytes. Staining was generally mild and not diffuse: negative hepatocytes were intermixed with positive ones. A positive intracytoplasmic immunostaining was occasionally observed in endothelial cells lining small blood vessels within AT septa and liver parenchyma. Our data confirm that bovine AT may provide an important source of SAA in healthy subjects. It remains to be determined which is the contribution of AT in the serum concentration of SAA.
authors: Department of Veterinary Sciences and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy. Electronic address: fabrizio.ceciliani@unimi.it.
authors: Fabrizio
authors: F
authors: Ceciliani
authors: UMR PRC - URA - INRA Val de Loire, 37380 Nouzilly, France.
authors: Laura
authors: L
authors: Soler
authors: Department of Veterinary Sciences and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors: Guido
authors: G
authors: Grilli
authors: Department of Veterinary Sciences and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors: Andreia T
authors: AT
authors: Marques
authors: Department of Veterinary Sciences and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors: Chiara
authors: C
authors: Giudice
authors: Department of Veterinary Sciences and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors: Cristina
authors: C
authors: Lecchi
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D000818:Animals; D002417:Cattle / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D007774:Lactation; D008099:Liver / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D000685:Serum Amyloid A Protein
keywords: Adipose tissues; Cow; Liver; Serum Amyloid A