title: [Serological diagnosis of sporotrichosis using an antigen of Sporothrix schenckii sensu stricto mycelium].
abstract: We developed and analyzed an Enzyme-Linked Immunosorbent Assay (ELISA) in order to detect antibodies in sera from sporotrichosis patients. We used a crude antigen of Sporothrix schenckii sensu stricto, obtained from the mycelial phase of the fungi. Positive sera were analyzed by other serological techniques such as double immunodiffusion (IGG) and counterimmunoelectrophoresis (CIE). The assay was validated by using sera from patients with other pathologies such as: histoplasmosis, paracoccidioidomycosis, tuberculosis, leishmaniasis, lupus and healthy individuals as negative controls. For the Sporothrix schenckii sensu stricto antigen, we found a 100% of specificity by every technique and sensitivity higher than 98% with IDD, CIE and ELISA. Our results show a high sensitivity and specificity for the Sporothrix schenckii sensu stricto antigen, so it can be used for IDD, CIE and ELISA. The results suggest that this antigen could be used in conjunction with other conventional tests for differential diagnosis and may be useful for monitoring the disease progression and response to treatment.
authors: Primavera
authors: P
authors: Alvarado
authors: Ana
authors: A
authors: Ostos
authors: Nohelys
authors: N
authors: Franquiz
authors: Antonio
authors: A
authors: Roschman-González
authors: Edgar A
authors: EA
authors: Zambrano
authors: Mireya
authors: M
authors: Mendoza
mesh_terms: D000946:Antigens, Fungal / Q000276:immunology; D003378:Counterimmunoelectrophoresis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D008297:Male; D025282:Mycelium; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013175:Sporothrix / Q000276:immunology / Q000302:isolation & purification*; D013174:Sporotrichosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000946:Antigens, Fungal