title: Bioanalytical and chemical sensors using living taste, olfactory, and neural cells and tissues: a short review.
abstract: Biosensors utilizing living tissues and cells have recently gained significant attention as functional devices for chemical sensing and biochemical analysis. These devices integrate biological components (i.e. single cells, cell networks, tissues) with micro-electro-mechanical systems (MEMS)-based sensors and transducers. Various types of cells and tissues derived from natural and bioengineered sources have been used as recognition and sensing elements, which are generally characterized by high sensitivity and specificity. This review summarizes the state of the art in tissue- and cell-based biosensing platforms with an emphasis on those using taste, olfactory, and neural cells and tissues. Many of these devices employ unique integration strategies and sensing schemes based on sensitive transducers including microelectrode arrays (MEAs), field effect transistors (FETs), and light-addressable potentiometric sensors (LAPSs). Several groups have coupled these hybrid biosensors with microfluidics which offers added benefits of small sample volumes and enhanced automation. While this technology is currently limited to lab settings due to the limited stability of living biological components, further research to enhance their robustness will enable these devices to be employed in field and clinical settings.
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China. cnpwang@zju.edu.cn.
authors: Chunsheng
authors: C
authors: Wu
authors: Peter B
authors: PB
authors: Lillehoj
authors: Ping
authors: P
authors: Wang
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000295:instrumentation / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation; D006801:Humans; D008027:Light; D055617:Micro-Electrical-Mechanical Systems; D044085:Microfluidics / Q000295:instrumentation; D016571:Neural Networks, Computer; D009474:Neurons / Q000378:metabolism*; D011199:Potentiometry / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012666:Semiconductors; D012903:Smell; D013649:Taste; D013696:Temperature; D014159:Transducers