title: Qualitative and quantitative evaluation of the BCR-ABL fusion gene in chronic myelogenous leukemia by flourescence in situ hybridization and molecular genetic methods.
abstract: OBJECTIVE
  Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We compared the diagnostic utility of FISH and QRT-PCR at various time points in the course of chronic myelogenous leukemia (CML) and to determine the mean initial values for patients whose QRT-PCR results were not known at the time of diagnosis.
  
  
  RESULTS
  We analyzed 135 results for 78 CML patients tested by FISH and QRT-PCR for the Ph chromosomal translocation. All newly diagnosed cases were positive by both methods. On follow-up following treatment, 1 case was FISH positive and QRT-PCR negative; 61 cases were FISH negative and QRT-PCR positive. Overall concordance was 54.1%. There was good concordance between QRT-PCR results and cytogenetic response categories.
  
  
  CONCLUSIONS
  We confirmed that QRT-PCR allows precise measurement of low levels of BCR-ABL transcripts and can serve as a sensitive indicator of MRD. We also demonstrated 100% correlation between QRT-PCR and FISH in newly diagnosed CML.
authors: 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, Turkey .
authors: Sebnem
authors: S
authors: Ozemri Sag
authors: 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, Turkey .
authors: Tahsin
authors: T
authors: Yakut
authors: 2 Department of Medical Genetics, Sevket Yılmaz Education and Research Hospital , Bursa, Turkey .
authors: Ozlem
authors: O
authors: Gorukmez
authors: 2 Department of Medical Genetics, Sevket Yılmaz Education and Research Hospital , Bursa, Turkey .
authors: Orhan
authors: O
authors: Gorukmez
authors: 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, Turkey .
authors: Mehmet
authors: M
authors: Ture
authors: 3 Department of Medical Genetics, Sakarya Education and Research Hospital , Sakarya, Turkey .
authors: Mutlu
authors: M
authors: Karkucak
authors: 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, Turkey .
authors: Tuna
authors: T
authors: Gulten
authors: 4 Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Uludag University , Bursa, Turkey .
authors: Ridvan
authors: R
authors: Ali
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000097:blood / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D016044:Fusion Proteins, bcr-abl