title: Gelatin hydrolysates from farmed Giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion.
abstract: This work aims to evaluate the ability of different alkaline proteases to prepare active gelatin hydrolysates. Fish skin gelatin was hydrolysed by visceral alkaline-proteases from Giant catfish, commercial trypsin, and Izyme AL®. All antioxidant activity indices of the hydrolysates increased with increasing degree of hydrolysis (P<0.05). The hydrolysates obtained with Izyme AL® and visceral alkaline-proteases showed the highest and lowest radical scavenging capacity, while prepared with commercial trypsin was the most effective in reducing ferric ions and showed the best metal chelating properties. The hydrolysate obtained with Izyme AL® showed the lowest iron reducing ability, but provided the highest average molecular weight (⩾ 7 kDa), followed by commercial trypsin (2.2 kDa) and visceral alkaline-proteases (1.75 kDa). After in vitro gastrointestinal digestion, the hydrolysates showed significant higher radical scavenging, reducing ferric ions and chelating activities. Gelatin hydrolysates, from fish skin, could serve as a potential source of functional food ingredients for health promotion.
authors: Program of Food Technology, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand.
authors: Sunantha
authors: S
authors: Ketnawa
authors: Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), José Antonio Novais, 10, Madrid 28040, Spain(1).
authors: Oscar
authors: O
authors: Martínez-Alvarez
authors: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors: Soottawat
authors: S
authors: Benjakul
authors: Program of Food Technology, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand. Electronic address: saroat@mfu.ac.th.
authors: Saroat
authors: S
authors: Rawdkuen
mesh_terms: D000818:Animals; D000975:Antioxidants; D001426:Bacterial Proteins / Q000737:chemistry*; D002397:Catfishes / Q000378:metabolism*; D004063:Digestion; D010450:Endopeptidases / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D011492:Protein Hydrolysates; D005780:Gelatin; D010450:Endopeptidases; C087822:alkaline protease
keywords: Alkaline proteases; Gastro-intestinal digestion; Gelatin; Giant catfish; Protein hydrolysates