title: Zhihengliuella somnathii sp. nov., a halotolerant actinobacterium from the rhizosphere of a halophyte Salicornia brachiata.
abstract: Two novel, Gram-stain-positive, rod-shaped, halotolerent bacteria, strains JG 03(T) and JG 05 were isolated from the rhizosphere of Salicornia brachiata, an extreme halophyte. Comparative analyses of 16S rRNA gene sequences showed that they were closely related to members of the genus Zhihengliuella, with sequence similarities of 96.9-99.1%. The sequence similarity of strains JG 03(T )and JG 05 with each other was 99.4%. DNA-DNA hybridization of JG 03(T) and JG 05 with other species of the genus Zhihengliuella with validly published names showed reassociation values of 19.8%-53.4% and a value of 91.4% between each other. The peptidoglycan type of both strains was A4α and MK-9 and MK-10 were the predominant menaquinones. The predominant fatty acid in JG 03(T) was anteiso-C15 : 0 and anteiso-C17 : 0. However, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids in strain JG 05. The DNA G+C content of strains JG 03(T) and JG 05 was 70.0 and 70.1 mol%, respectively. In nutrient broth medium both strains grew at NaCl concentrations of up to 15% (w/v). On the basis of chemotaxonomic characteristics and phylogenetic analyses, strains JG 03(T) and JG 05 should be affiliated to the genus Zhihengliuella. Strains JG 03(T) and JG 05 represent a novel species of the genus Zhihengliuella for which the name Zhihengliuella somnathii sp. nov. is proposed. The type strain is JG 03(T) ( = DSM 23187(T) = IMCC 253(T)).
authors: Academy of Scientific and Innovative Research, CSIR, New Delhi, India.|Marine Biotechnology and Ecology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar-364002, Gujarat, India.
authors: Bhavanath
authors: B
authors: Jha
authors: Marine Biotechnology and Ecology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar-364002, Gujarat, India.
authors: Vijay
authors: V
authors: Kumar Singh
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department for Environmental Sciences, Research Unit Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany.
authors: Angelo
authors: A
authors: Weiss
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department for Environmental Sciences, Research Unit Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany.
authors: Anton
authors: A
authors: Hartmann
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department for Environmental Sciences, Research Unit Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany.
authors: Michael
authors: M
authors: Schmid
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019663:Chenopodiaceae; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008835:Micrococcaceae / Q000145:classification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010457:Peptidoglycan; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D055051:Salt-Tolerant Plants; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride