title: Molecular Analysis of Mouse T Cell Receptor α and β Gene Rearrangements.
abstract: PCR on genomic DNA isolated from lymphocyte populations is an invaluable technique to analyze T cell receptor (TCR) α and β gene rearrangements. Although this approach is powerful, it also has limitations that must be accounted for in experimental design and data interpretation. Here, we provide background required for understanding these limitations, and then outline standard PCR methods that can be used for analysis of TCRα and β gene rearrangements in mice.
authors: Division of Cancer Pathobiology, Department of Pathology and Laboratory Medicine, Center for Childhood Cancer Research, Children's Hospital of Philadelphia, 4054 Colket Translational Research Building, 3501 Civic Center Blvd, Philadelphia, PA, 19104, USA.
authors: Levi J
authors: LJ
authors: Rupp
authors: Liang
authors: L
authors: Chen
authors: Michael S
authors: MS
authors: Krangel
authors: Craig H
authors: CH
authors: Bassing
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002454:Cell Differentiation / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte; D056426:Genetic Loci; D051379:Mice; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta