title: Preparation and characterization of bioadhesive controlled-release gels of cidofovir for vaginal delivery.
abstract: The aim of this study was to develop mucoadhesive and thermosensitive gels for vaginal delivery that would be able to provide a controlled release of the model drug, cidofovir. The study also monitored the drug's potential antiviral properties. Cidofovir was put into the form of a vaginal gel, using mucoadhesive and thermosensitive polymers such as chitosan, Carbopol 974P, HPMC, and poloxamer 407. The physicopharmaceutical properties and stability of the vaginal gel formulations were evaluated. The gel formulation which was prepared with HPMC K100M exhibited the highest viscosity, as well as maximum adhesiveness, cohesiveness, and mucoadhesion values. The results of antiviral activity studies, which used the bovine herpes virus type 1 virus infection in vitro model using Vero cells, demonstrated the antiherpetic effect of the cidofovir gel containing HPMC K100M, at least under in vitro conditions. The study found that a mucoadhesive vaginal gel containing cidofovir can be a promising and innovative alternative therapeutic system for the treatment of genital herpes simplex virus and human papilloma virus induced infections in women.
authors: a Faculty of Pharmacy, Department of Pharmaceutical Technology , Gazi University , 06330-Hipodrom, Ankara , Turkey.
authors: Fatmanur
authors: F
authors: Tuğcu-Demiröz
authors: a Faculty of Pharmacy, Department of Pharmaceutical Technology , Gazi University , 06330-Hipodrom, Ankara , Turkey.
authors: Füsun
authors: F
authors: Acartürk
authors: b Faculty of Veterinary Medicine, Department of Virology , Ankara University , Dışkapı, 06110 Ankara , Turkey.
authors: Aykut
authors: A
authors: Özkul
mesh_terms: D000268:Adhesiveness; D000269:Adhesives / Q000737:chemistry* / Q000633:toxicity; D000282:Administration, Intravaginal; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D002522:Chlorocebus aethiops; D000077404:Cidofovir; D003596:Cytosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D004355:Drug Stability; D005782:Gels; D007242:Herpesvirus 1, Bovine / Q000187:drug effects; D009092:Mucous Membrane / Q000737:chemistry; D063065:Organophosphonates / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D014709:Vero Cells
chemical_list: D000269:Adhesives; D000998:Antiviral Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005782:Gels; D063065:Organophosphonates; D003596:Cytosine; D000077404:Cidofovir
keywords: HPMC; carbopol; cytotoxicity; genital herpes simplex; human papilloma virus; mucoadhesive polymers; texture profile analysis; thermosensitive polymer