title: Rapid non-invasive genotyping of reporter transgenic mammals.
abstract: Here we describe a non-invasive method for rapid and highly reproducible genotyping of transgenic mammals with ubiquitous expression of fluorophore reporters. Hair samples from transgenic mice and pigs with systemic expression of the fluorophore reporter Venus were analyzed with a fluorescence microscope in few minutes. The hair samples can be preserved for long-term storage at ambient temperature conditions. This non-invasive method is useful for genotyping of transgenic large animals and contributes to animal welfare by reducing stress and discomfort of the animals during sample collection.
authors: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Mariensee, Germany.
authors: Wiebke
authors: W
authors: Garrels
authors: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Mariensee, Germany.
authors: Nicole
authors: N
authors: Cleve
authors: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Mariensee, Germany.
authors: Heiner
authors: H
authors: Niemann
authors: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Mariensee, Germany.
authors: Wilfried A
authors: WA
authors: Kues
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D060005:Genotyping Techniques / Q000379:methods*; D006197:Hair / Q000737:chemistry; D015603:Keratinocytes; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria
keywords: Sleeping Beauty transposon; animal welfare; cornified keratinocyte; expression; extracellular; fluorescent protein; keratin; phenotype; ubiquitous