title: Desalted Duck Egg White Peptides: Promotion of Calcium Uptake and Structure Characterization.
abstract: The effects of desalted duck egg white peptides (DPs) on calcium absorption were investigated in three models: Caco-2 cell monolayer model, Caco-2 cell population model, and everted intestinal sac model. DPs were found to enhance calcium transport and may do so by acting as calcium carriers and interacting with the cell membrane to open a special Ca(2+) channel, whereas the paracellular pathway may make only a minor contribution. Structure characterization demonstrated the important roles of seven crucial peptides, such as VSEE and LYAEE, in binding calcium and promoting calcium uptake. Three synthetic peptides (VHSS, VSEE, and VHS(p)S(p)) potently induced calcium transport in Caco-2 monolayers, with VHS(p)S(p) being the most effective. This research expands the understanding of the mechanism of cellular calcium uptake by DPs as well as highlights an opportunity for recycling an otherwise discarded processing byproduct.
authors: College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.|Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
authors: Tao
authors: T
authors: Hou
authors: College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.|Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
authors: Chi
authors: C
authors: Wang
authors: College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.|Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
authors: Zhili
authors: Z
authors: Ma
authors: College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.|Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
authors: Wen
authors: W
authors: Shi
authors: College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.|Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
authors: Lui
authors: L
authors: Weiwei
authors: College of Food Science and Technology, Huazhong Agricultural University , Wuhan 430070, China.|Key Laboratory of Environment Correlative Dietology ( Huazhong Agricultural University) , Ministry of Education, Wuhan 43000, China.
authors: Hui
authors: H
authors: He
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism* / Q000493:pharmacokinetics*; D002136:Calcium, Dietary / Q000008:administration & dosage; D002462:Cell Membrane; D004372:Ducks; D004527:Egg Proteins / Q000737:chemistry* / Q000494:pharmacology*; D005511:Food Handling / Q000379:methods; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects; D059027:Recycling; D012965:Sodium Chloride
chemical_list: D002136:Calcium, Dietary; D004527:Egg Proteins; D012965:Sodium Chloride; D002118:Calcium
keywords: Caco-2 cells; calcium uptake; desalted duck egg white peptides (DPs); everted intestinal sac model; structure characterization