title: [Ethyl acetate-soluble chemical constituents from branch of Hypericum petiolulatum].
abstract: The stems and branches of Hypericum petiolulatum were extracted by alcohol and liquid-liquid extraction. Seven furofuran lignans were isolated from the ethyl acetate fraction of ethanol extract of H. petiolulatum by using silica gelchromatography, Sephadex LH-20 chromatography, medium-pressure liquid chromatography and preparative HPLC. Their structures were identified by the spectroscopic methods as pinoresinol (1), medioresinol (2), 8-acetoxypinoresinol (3), epipinoresinol (4), (+)-syringaresinol (5), (+)-1-hydroxysyringaresinol (6) and erythro-buddlenolE (7). All the isolates were firstly found in H. petiolulatum. In the bioassay, compound 7 showed remarkable antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate 38% at a concentration of 1 x 10(-6) mol · L(-1) (positive control Vit E with the inhibitory rate of 35% at the same concentration).
authors: Qi
authors: Q
authors: Zhao
authors: Hai-long
authors: HL
authors: Zhang
authors: Xue
authors: X
authors: Zhang
authors: Xiao-meng
authors: XM
authors: Li
authors: Yu-zhen
authors: YZ
authors: Sun
authors: Hang
authors: H
authors: Su
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020902:Hypericum / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D018547:Plant Stems / Q000737:chemistry; D051381:Rats
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal