title: miR-125a-5p inhibits cell proliferation and induces apoptosis in colon cancer via targeting BCL2, BCL2L12 and MCL1.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that function as regulators of gene expression. MiR-125 is a family of miRNAs that have been shown to be involved in various cancer types. In this study, for the first time, we showed that miR-125a-5p was specifically down-regulated in both colon cancer tissue and colon cancer cell lines. The tumor suppressor role of miR-125a-5p in colon cancer was supported by the observation that overexpression of miR-125a-5p inhibited cell proliferation and induced cell apoptosis in colon cancer cells. We also confirmed that in colon cancer cells, anti-apoptotic genes BCL2, BCL2L12 and Mcl-1 were direct targets of miR-125a-5p, and they were down-regulated by miR-125a-5p overexpression. Furthermore, restoration of BCL2, BCL2L12 and Mcl-1 expression in colon cancer cells could partially reverse the cell proliferation inhibition and apoptosis stimulation caused by miR-125a-5p overexpression, indicating that miR-125a-5p inhibits cell proliferation and induces apoptosis in colon cancer cells via targeting BCL2, BCL2L12 and Mcl-1.
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China.
authors: Zhiguo
authors: Z
authors: Tong
authors: Department of Pathology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng St. Nangang, Harbin 150006, Heilongjiang, China.
authors: Nan
authors: N
authors: Liu
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China.
authors: Luoqiang
authors: L
authors: Lin
authors: Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China.
authors: Xinggang
authors: X
authors: Guo
authors: Department of Surgical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China.
authors: Dongdong
authors: D
authors: Yang
authors: Department of Surgical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China. Electronic address: qifanzhang2015@163.com.
authors: Qifan
authors: Q
authors: Zhang
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D001665:Binding Sites; D018938:Caco-2 Cells; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C000595707:BCL2 protein, human; C430100:BCL2L12 protein, human; C579095:MCL1 protein, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D009124:Muscle Proteins; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: BCL2; BCL2L12; Colon cancer; Mcl-1; miR-125a-5p