title: Yeast Pmp3p has an important role in plasma membrane organization.
abstract: Pmp3p-related proteins are highly conserved proteins that exist in bacteria, yeast, nematodes and plants, and its transcript is regulated in response to abiotic stresses, such as low temperature or high salinity. Pmp3p was originally identified in Saccharomyces cerevisiae, and it belongs to the sensitive to Na(+) (SNA)-protein family, which comprises four members--Pmp3p/Sna1p, Sna2p, Sna3p and Sna4p. Deletion of the PMP3 gene conferred sensitivity to cytotoxic cations, whereas removal of the other SNA genes did not lead to clear phenotypic effects. It has long been believed that Pmp3p-related proteins have a common and important role in the modulation of plasma membrane potential and in the regulation of intracellular ion homeostasis. Here, we show that several growth phenotypes linked to PMP3 deletion can be modulated by the removal of specific genes involved in sphingolipid synthesis. These genetic interactions, together with lipid binding assays and epifluorescence microscopy, as well as other biochemical experiments, suggest that Pmp3p could be part of a phosphoinositide-regulated stress sensor.
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium.
authors: Julien
authors: J
authors: De Block
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium.
authors: Aleksandra
authors: A
authors: Szopinska
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium.
authors: Bérengère
authors: B
authors: Guerriat
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium.
authors: Joanna
authors: J
authors: Dodzian
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium.
authors: Jennifer
authors: J
authors: Villers
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium.
authors: Jean-François
authors: JF
authors: Hochstenbach
authors: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, Louvain-la-Neuve B-1348, Belgium pierre.morsomme@uclouvain.be.
authors: Pierre
authors: P
authors: Morsomme
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008565:Membrane Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013107:Sphingolipids / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids
keywords: Phosphoinositide; Proteolipid; SNA; Sphingolipid