title: Identification and Characterization of the Major Chemical Constituents in Fructus Akebiae by High-Performance Liquid Chromatography Coupled with Electrospray Ionization-Quadrupole-Time-of-Flight Mass Spectrometry.
abstract: Fructus Akebiae (FA), the dry fruit of Akebia quinata (THUNB.) DECNE., possesses potent antidepressant properties. Owing to the structural complexity, high polarity and thermal lability in plants, it is difficult and time-consuming to analyze the major chemical constituents by traditional strategies that involve extraction, isolation, purification and identification by chemical manipulations and spectroscopic methods. In this study, a high-performance liquid chromatography coupled with electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC-ESI-Q-TOF-MS-MS) method was established for quickly identifying the chemical constituents in the extract of Fructus Akebiae. The main saponin components in the extract of Fructus Akebiae were detected with the HPLC-ESI-Q-TOF-MS-MS in negative-ion mode. These components were further analyzed by MS(2) spectra, and compared with the corresponding reference substances and literature data. Nineteen saponins in the extract of Fructus Akebiae were well separated in one run. The new method is accurate and rapid. It can be used to identify the main chemical constituents in the extract of Fructus Akebiae and can be suitable for the quality control of Fructus Akebiae.
authors: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China lymanuxt@126.com 13970209819@163.com.
authors: Yun
authors: Y
authors: Ling
authors: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
authors: Qing
authors: Q
authors: Zhang
authors: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
authors: Dan-dan
authors: DD
authors: Zhu
authors: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
authors: Fei
authors: F
authors: Chen
authors: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
authors: Xiu-hua
authors: XH
authors: Kong
authors: Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China lymanuxt@126.com 13970209819@163.com.
authors: Liang
authors: L
authors: Liao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C571811:Fructus Akebiae; D010936:Plant Extracts