title: Non-malignant T-cells lacking multiple pan-T markers can be found in lymph nodes.
abstract: In order to observe and ascertain the properties of a sub-group of T cells in the lymph node (LN) from seven patients who did not suffer from T cell lymphoproliferative disorders (T-LPDs), the expression levels of several pan-T markers were evaluated by multiparameter flow cytometry (FC) and the clonality of these T-cells was evaluated by both FC analysis and PCR assessment. It turned out that multiple pan-T-cell markers such as CD2, CD5 and CD7 were found to be lost in these T cells. The majority of them were positive for TCRαβ, only a minority of them being positive for TCRγδ. A subset of these T-cells were positive for CD4 or CD8 or dual-negative for CD4 and CD8. Oligoclonality was detected in one case by FC, while clonal TCR rearrangement was detected in three cases. Absence of multiple pan-T-cell markers could be found in benign T cells in LNs.
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Wei
authors: W
authors: Wang
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Li
authors: L
authors: Gao
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Ming
authors: M
authors: Gong
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Yin
authors: Y
authors: Tang
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Yan
authors: Y
authors: Li
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Wen-Tao
authors: WT
authors: Zhang
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Fan-Zhou
authors: FZ
authors: Huang
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Chun-Xia
authors: CX
authors: Zhang
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Yan-Rong
authors: YR
authors: Chen
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Ya-Yue
authors: YY
authors: Gao
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Zhen-Ling
authors: ZL
authors: Li
authors: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors: Yi-Gai
authors: YG
authors: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008198:Lymph Nodes / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Flow cytometry; benign T cells; lacking pan-T-cell markers; lymph node