title: [Suppression of E3 ubiquitin ligase Cbl-b in interleukin-1 signaling].
abstract: The present study aims to investigate the effect of Cbl-b, a member of E3 ubiquitin ligase family, on interleukin-1 (IL-1) pathway in synoviocytes. The protein expression levels of Cbl-b and IL-1-induced matrix metalloproteinase 13 (MMP-13) in synoviocytes were analyzed by Western blot. Collagen substrates were incubated with the conditioned medium collected from synoviocytes cultures and then subjected to SDS-PAGE for analysis of collagen degradation. The results showed that compared with wild-type cells, Cbl-b-deficient cells expressed more MMP-13 protein and had enhanced ability to degrade collagens under IL-1 stimulation. These data suggest that Cbl-b may negatively regulate IL-1-triggered degradation of collagen matrix in synoviocytes.
authors: Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710032, China.
authors: Jiang-Tian
authors: JT
authors: Yu
authors: Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710032, China.
authors: Xin
authors: X
authors: Bu
authors: Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710032, China.
authors: Hu
authors: H
authors: Zhao
authors: Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710032, China. sujin923@fmmu.edu.cn.
authors: Jin
authors: J
authors: Su
mesh_terms: D003094:Collagen / Q000378:metabolism; D004847:Epithelial Cells / Q000201:enzymology*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D050721:Proto-Oncogene Proteins c-cbl / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D007375:Interleukin-1; D003094:Collagen; D050721:Proto-Oncogene Proteins c-cbl; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13