title: Using the synergism strategy for highly sensitive and specific electrochemical sensing of Streptococcus pneumoniae Lyt-1 gene sequence.
abstract: With the help of the interaction mode of capture probe-target-signal probe (CP-T-SP), an electrochemical sensing method based on the synergism strategy of dual-hybridized signaling probes modified with 6 MB (methylene blue), background suppression and large surface area Au electrode is developed for the detection of Streptococcus pneumoniae (S. pneumoniae) Lyt-1 gene sequence. The proposed sensor features a very low detection limit (LOD) of ∼0.5 fM for the target. This method also exhibits highly versatility and can apply to the construction of other sensors for the analysis of similar designated pathogenic bacteria gene sequence (PBGS).
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors: Fengqin
authors: F
authors: Li
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China. Electronic address: yzgyhdzm@126.com.
authors: Zhigang
authors: Z
authors: Yu
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors: Yanmei
authors: Y
authors: Xu
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors: Huiyuan
authors: H
authors: Ma
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors: Guiling
authors: G
authors: Zhang
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors: Yongbin
authors: Y
authors: Song
authors: College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China. Electronic address: yanhong204821@aliyun.com.
authors: Hong
authors: H
authors: Yan
authors: Department of Electronic Science and Technology, Harbin University of Science and Technology, Harbin 150080, China.
authors: Xunjun
authors: X
authors: He
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005798:Genes, Bacterial; D006046:Gold / Q000737:chemistry; D006801:Humans; D011008:Pneumococcal Infections / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D006046:Gold
keywords: Electrochemical sensing method; Gene sequence; Mismatch discrimination; Streptococcus pneumoniae; Synergism strategy