title: Manufacturing of dental pulp cell-based products from human third molars: current strategies and future investigations.
abstract: In recent years, mesenchymal cell-based products have been developed to improve surgical therapies aimed at repairing human tissues. In this context, the tooth has recently emerged as a valuable source of stem/progenitor cells for regenerating orofacial tissues, with easy access to pulp tissue and high differentiation potential of dental pulp mesenchymal cells. International guidelines now recommend the use of standardized procedures for cell isolation, storage and expansion in culture to ensure optimal reproducibility, efficacy and safety when cells are used for clinical application. However, most dental pulp cell-based medicinal products manufacturing procedures may not be fully satisfactory since they could alter the cells biological properties and the quality of derived products. Cell isolation, enrichment and cryopreservation procedures combined to long-term expansion in culture media containing xeno- and allogeneic components are known to affect cell phenotype, viability, proliferation and differentiation capacities. This article focuses on current manufacturing strategies of dental pulp cell-based medicinal products and proposes a new protocol to improve efficiency, reproducibility and safety of these strategies.
authors: Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique, UMR5305 Centre National de la Recherche Scientifique/Université Claude Bernard Lyon 1 Lyon, France ; Faculté d'Odontologie, Université de Lyon, Université Claude Bernard Lyon 1 Lyon, France ; Hospices Civils de Lyon, Service de Consultations et Traitements Dentaires Lyon, France.
authors: Maxime
authors: M
authors: Ducret
authors: Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique, UMR5305 Centre National de la Recherche Scientifique/Université Claude Bernard Lyon 1 Lyon, France.
authors: Hugo
authors: H
authors: Fabre
authors: CTI-BIOTECH, Cell Therapy Research Institute Meyzieu, France.
authors: Olivier
authors: O
authors: Degoul
authors: CTI-BIOTECH, Cell Therapy Research Institute Meyzieu, France.
authors: Gianluigi
authors: G
authors: Atzeni
authors: CTI-BIOTECH, Cell Therapy Research Institute Meyzieu, France.
authors: Colin
authors: C
authors: McGuckin
authors: CTI-BIOTECH, Cell Therapy Research Institute Meyzieu, France.
authors: Nico
authors: N
authors: Forraz
authors: Faculté d'Odontologie, Institut National de la Santé et de la Recherche Médicale UMR1064, Centre de Recherche en Transplantation et Immunologie, Université de Nantes Nantes, France.
authors: Brigitte
authors: B
authors: Alliot-Licht
authors: Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique, UMR5305 Centre National de la Recherche Scientifique/Université Claude Bernard Lyon 1 Lyon, France.
authors: Frédéric
authors: F
authors: Mallein-Gerin
authors: Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique, UMR5305 Centre National de la Recherche Scientifique/Université Claude Bernard Lyon 1 Lyon, France.
authors: Emeline
authors: E
authors: Perrier-Groult
authors: Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique, UMR5305 Centre National de la Recherche Scientifique/Université Claude Bernard Lyon 1 Lyon, France ; Faculté d'Odontologie, Université de Lyon, Université Claude Bernard Lyon 1 Lyon, France ; Hospices Civils de Lyon, Service de Consultations et Traitements Dentaires Lyon, France.
authors: Jean-Christophe
authors: JC
authors: Farges
keywords: cell-based medicinal products; cryopreservation; expansion; good manufacturing practices; human dental pulp; immunophenotyping; stem cells; tissue engineering