title: The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.
abstract: To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Lizabeth A
authors: LA
authors: Perkins
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Laura
authors: L
authors: Holderbaum
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Rong
authors: R
authors: Tao
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Yanhui
authors: Y
authors: Hu
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Richelle
authors: R
authors: Sopko
authors: Boston University, Boston, Massachusetts 02215.
authors: Kim
authors: K
authors: McCall
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Donghui
authors: D
authors: Yang-Zhou
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Ian
authors: I
authors: Flockhart
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 Howard Hughes Medical Institute, Boston, Massachusetts 02115.
authors: Richard
authors: R
authors: Binari
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Hye-Seok
authors: HS
authors: Shim
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Audrey
authors: A
authors: Miller
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Amy
authors: A
authors: Housden
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Marianna
authors: M
authors: Foos
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Sakara
authors: S
authors: Randkelv
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Colleen
authors: C
authors: Kelley
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Pema
authors: P
authors: Namgyal
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 Howard Hughes Medical Institute, Boston, Massachusetts 02115.
authors: Christians
authors: C
authors: Villalta
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 TsingHua Fly Center, Beijing, 100084, China.
authors: Lu-Ping
authors: LP
authors: Liu
authors: TsingHua Fly Center, Beijing, 100084, China.
authors: Xia
authors: X
authors: Jiang
authors: TsingHua Fly Center, Beijing, 100084, China.
authors: Qiao
authors: Q
authors: Huan-Huan
authors: TsingHua Fly Center, Beijing, 100084, China.
authors: Xia
authors: X
authors: Wang
authors: Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan.
authors: Asao
authors: A
authors: Fujiyama
authors: Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan.
authors: Atsushi
authors: A
authors: Toyoda
authors: Department of Genetics, Yale University, New Haven, Connecticut 06510.
authors: Kathleen
authors: K
authors: Ayers
authors: Howard Hughes Medical Institute, Boston, Massachusetts 02115 Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016.
authors: Allison
authors: A
authors: Blum
authors: CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom.
authors: Benjamin
authors: B
authors: Czech
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Ralph
authors: R
authors: Neumuller
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Dong
authors: D
authors: Yan
authors: Howard Hughes Medical Institute, Boston, Massachusetts 02115 Janelia Farm Research Institute ,Asburn, Virginia, 20147.
authors: Amanda
authors: A
authors: Cavallaro
authors: Howard Hughes Medical Institute, Boston, Massachusetts 02115 Janelia Farm Research Institute ,Asburn, Virginia, 20147.
authors: Karen
authors: K
authors: Hibbard
authors: Howard Hughes Medical Institute, Boston, Massachusetts 02115 Janelia Farm Research Institute ,Asburn, Virginia, 20147.
authors: Don
authors: D
authors: Hall
authors: Department of Genetics, Yale University, New Haven, Connecticut 06510.
authors: Lynn
authors: L
authors: Cooley
authors: CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom.
authors: Gregory J
authors: GJ
authors: Hannon
authors: Howard Hughes Medical Institute, Boston, Massachusetts 02115 Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016.
authors: Ruth
authors: R
authors: Lehmann
authors: Bloomington Drosophila Stock Center Bloomington, Indiana, 47405.
authors: Annette
authors: A
authors: Parks
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors: Stephanie E
authors: SE
authors: Mohr
authors: Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan.
authors: Ryu
authors: R
authors: Ueda
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan.
authors: Shu
authors: S
authors: Kondo
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 TsingHua Fly Center, Beijing, 100084, China.
authors: Jian-Quan
authors: JQ
authors: Ni
authors: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 Howard Hughes Medical Institute, Boston, Massachusetts 02115 perrimon@receptor.med.harvard.edu.
authors: Norbert
authors: N
authors: Perrimon
mesh_terms: D022126:Access to Information; D000818:Animals; D030801:Animals, Genetically Modified; D035843:Biomedical Research; D001900:Boston; D004330:Drosophila / Q000235:genetics*; D017344:Genes, Insect; D005822:Genetic Vectors; D034622:RNA Interference; D012577:Schools, Medical
keywords: Drosophila; RNAi; functional genomics; phenotypes; screens