title: Diagnostic value of SOX-10 immunohistochemical staining for the detection of uveal melanoma.
abstract: OBJECTIVE
  SOX-10 has been shown to be a sensitive marker of cutaneous melanoma. This study aimed to evaluate Sox-10 expression in uveal melanoma.
  
  
  METHODS
  A total of 40 tissue blocks of enucleated eyes with uveal melanoma were cut and stained using an anti-SOX-10 mouse monoclonal antibody and HMB-45 antibody.
  
  
  RESULTS
  SOX-10 showed exclusive nuclear positivity in 100% of the uveal melanoma cases (38/38). HMB-45 showed cytoplasmic positivity in 97.3 (37/38). Positivity for SOX-10 was also noted in the inner and outer nuclear layers of the retina in 78% of the enucleated eyes.
  
  
  CONCLUSIONS
  SOX-10 expression proved to be the most sensitive marker for uveal melanoma, and therefore, we propose a modified panel for the diagnosis of uveal melanoma that includes both SOX-10 and HMB-45. The observation of distinct, diffuse nuclear SOX-10 expression in retinal inner and outer nuclear layers is a finding that warrants further investigation as a marker for retinoblastoma.
authors: Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
authors: Sarah A
authors: SA
authors: Alghamdi
authors: Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
authors: Pablo
authors: P
authors: Zoroquiain
authors: Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
authors: Ana Beatriz T
authors: AB
authors: Dias
authors: Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
authors: Sulaiman R
authors: SR
authors: Alhumaid
authors: Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
authors: Sultan
authors: S
authors: Aldrees
authors: Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
authors: Miguel N
authors: MN
authors: Burnier
keywords: SOX-10; immunohistochemistry; uveal melanoma