title: Simultaneous Determination of Type A and B Trichothecenes and Their Main Metabolites in Food Animal Tissues by Ultraperformance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry.
abstract: A rapid method for the liquid chromatography-tandem mass spectrometric determination of type A and B trichothecenes and their major metabolites in chicken meat, pork, chicken liver, and swine liver was developed. The analytes included T-2 toxin, HT-2 toxin, T-2 triol, neosolaniol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deepoxydeoxynivalenol, and nivalenol. The compounds were extracted from samples with acetonitrile/ethyl acetate (1:3, v/v) and then cleaned up using Oasis HLB cartridges. Analysis was carried out with ultraperformance liquid chromatography-tandem mass spectrometry. The mean recoveries of spiked samples ranged from 74.1% to 96.9% with intraday and interday relative standard deviations of less than 9.9% and 9.1%, respectively. The limit of detection and limit of quantitation ranged from 3.0 to 15.0 μg/kg and from 10.0 to 50.0 μg/kg, respectively. The proposed method has been successfully applied for analysis of real samples, with the primary results indicating that, compared to mycotoxins themselves, their metabolites are more likely to occur and be detectable in animal tissue foods.
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Shupeng
authors: S
authors: Yang
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Ying
authors: Y
authors: Wang
authors: Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture , College Station, Texas 77845, United States.
authors: Ross C
authors: RC
authors: Beier
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Huiyan
authors: H
authors: Zhang
authors: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University , Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors: Karl
authors: K
authors: De Ruyck
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Feifei
authors: F
authors: Sun
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Xingyuan
authors: X
authors: Cao
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Jianzhong
authors: J
authors: Shen
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Suxia
authors: S
authors: Zhang
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.
authors: Zhanhui
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D009183:Mycotoxins / Q000737:chemistry* / Q000378:metabolism; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014255:Trichothecenes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes
keywords: DON; T-2 toxin; chicken; liquid chromatography; liver; metabolites; pork; tandem mass spectrometry; type A and B trichothecenes