title: Klf10 regulates odontoblast differentiation and mineralization via promoting expression of dentin matrix protein 1 and dentin sialophosphoprotein genes.
abstract: Klf10, a member of the Krüppel-like family of transcription factors, is critical for osteoblast differentiation, bone formation and mineralization. However, whether Klf10 is involved in odontoblastic differentiation and tooth development has not been determined. In this study, we investigate the expression patterns of Klf10 during murine tooth development in vivo and its role in odontoblastic differentiation in vitro. Klf10 protein was expressed in the enamel organ and the underlying mesenchyme, ameloblasts and odontoblasts at early and later stages of murine molar formation. Furthermore, the expression of Klf10, Dmp1, Dspp and Runx2 was significantly elevated during the process of mouse dental papilla mesenchymal differentiation and mineralization. The overexpression of Klf10 induced dental papilla mesenchymal cell differentiation and mineralization as detected by alkaline phosphatase staining and alizarin red S assay. Klf10 additionally up-regulated the expression of odontoblastic differentiation marker genes Dmp1, Dspp and Runx2 in mouse dental papilla mesenchymal cells. The molecular mechanism of Klf10 in controlling Dmp1 and Dspp expression is thus to activate their regulatory regions in a dosage-dependent manner. Our results suggest that Klf10 is involved in tooth development and promotes odontoblastic differentiation via the up-regulation of Dmp1 and Dspp transcription.
authors: Department of Conservative Dentistry, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, People's Republic of China.|Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Tex., USA.
authors: Zhuo
authors: Z
authors: Chen
authors: Department of Pathology, Weifang Medical University, Weifang, Shandong, People's Republic of China.
authors: Wentong
authors: W
authors: Li
authors: Shangyang Dental Clinic, Hangzhou, People's Republic of China.
authors: Han
authors: H
authors: Wang
authors: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Tex., USA.
authors: Chunyan
authors: C
authors: Wan
authors: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Tex., USA.
authors: Daoshu
authors: D
authors: Luo
authors: Department of Conservative Dentistry, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, People's Republic of China.
authors: Shuli
authors: S
authors: Deng
authors: Department of Conservative Dentistry, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, People's Republic of China. huic66@hotmail.com.
authors: Hui
authors: H
authors: Chen
authors: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Tex., USA. chens0@uthscsa.edu.
authors: Shuo
authors: S
authors: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics*; D003771:Dental Papilla / Q000166:cytology; D051765:Early Growth Response Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008963:Molar / Q000196:embryology / Q000378:metabolism; D009804:Odontoblasts / Q000166:cytology*; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D015415:Biomarkers; D051765:Early Growth Response Transcription Factors; D016326:Extracellular Matrix Proteins; D051741:Kruppel-Like Transcription Factors; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513308:Dmp1 protein, mouse; C569958:KLF10 protein, mouse
keywords: Dmp1; Dspp; Klf10; Odontoblast; Tooth development