title: The effects of graded levels of calorie restriction: IV. Non-linear change in behavioural phenotype of mice in response to short-term calorie restriction.
abstract: Animals have to adjust their activities when faced with caloric restriction (CR) to deal with reduced energy intake. If CR is pronounced, allostasis can push individuals into alternate physiological states which can result in important health benefits across a wide range of taxa. Here we developed a new approach to determine the changes in behavioural phenotype associated with different levels of CR. We exposed C57BL/6 male mice to graded CR (from 0 to 40%) for three months and defined their behavioural phenotype using hidden Markov models of their movement and body temperature. All 40% CR mice exhibited a state-shift in behavioural phenotype and only some exposed to 30% CR did. We show for the first time that mice changed their activity characteristics rather than changed their activities. This new phenotyping approach provides an avenue to determine the mechanisms linking CR to healthspan.
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
authors: David
authors: D
authors: Lusseau
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
authors: Sharon E
authors: SE
authors: Mitchell
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
authors: Ceres
authors: C
authors: Barros
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
authors: Davina
authors: D
authors: Derous
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
authors: Cara
authors: C
authors: Green
authors: Key laboratory of Systems Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 800 Cao Bao road, Shanghai, China.
authors: Luonan
authors: L
authors: Chen
authors: Max Planck partner institute for computational biology, Shanghai.
authors: Jing-Dong Jackie
authors: JD
authors: Han
authors: State Key laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 Beichen Xilu, Chaoyang, Beijing, China.
authors: Yingchun
authors: Y
authors: Wang
authors: Department of Pathology, University of Washington at Seattle, Seattle, Washington, USA.
authors: Daniel E L
authors: DE
authors: Promislow
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.
authors: Alex
authors: A
authors: Douglas
authors: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK Ab24 2TZ.|State Key laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 Beichen Xilu, Chaoyang, Beijing, China.
authors: John R
authors: JR
authors: Speakman
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001833:Body Temperature Regulation / Q000502:physiology*; D031204:Caloric Restriction / Q000379:methods*; D003198:Computer Simulation; D004435:Eating / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D015233:Models, Statistical; D009043:Motor Activity / Q000502:physiology; D017711:Nonlinear Dynamics