title: Nmdmc overexpression extends Drosophila lifespan and reduces levels of mitochondrial reactive oxygen species.
abstract: NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase (NMDMC) is a bifunctional enzyme involved in folate-dependent metabolism and highly expressed in rapidly proliferating cells. However, Nmdmc physiological roles remain unveiled. We found that ubiquitous Nmdmc overexpression enhanced Drosophila lifespan and stress resistance. Interestingly, Nmdmc overexpression in the fat body was sufficient to increase lifespan and tolerance against oxidative stress. In addition, these conditions coincided with significant decreases in the levels of mitochondrial ROS and Hsp22 as well as with a significant increase in the copy number of mitochondrial DNA. These results suggest that Nmdmc overexpression should be beneficial for mitochondrial homeostasis and increasing lifespan.
authors: Department of Preventive Medicine, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors: Suyeun
authors: S
authors: Yu
authors: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors: Yeogil
authors: Y
authors: Jang
authors: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors: Donggi
authors: D
authors: Paik
authors: Department of Preventive Medicine, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea. Electronic address: eunil@korea.ac.kr.
authors: Eunil
authors: E
authors: Lee
authors: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea. Electronic address: parkjj@korea.ac.kr.
authors: Joong-Jean
authors: JJ
authors: Park
mesh_terms: D000619:Aminohydrolases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism; D005260:Female; D017344:Genes, Insect; D008136:Longevity / Q000235:genetics* / Q000502:physiology*; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics* / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D004272:DNA, Mitochondrial; D029721:Drosophila Proteins; D009097:Multienzyme Complexes; D017382:Reactive Oxygen Species; C055342:methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases
keywords: Drosophila; Longevity; Mitochondrial DNA; NMDMC; Reactive oxygen species