title: Pseudoclavibacter terrae sp. nov. isolated from rhizosphere soil of Ophiopogon japonicus.
abstract: Strain THG-MD12T, a Gram-reaction-positive, aerobic, non-motile, rod-shaped bacterium was isolated from rhizosphere soil of Ophiopogon japonicus in PR China. THG-MD12T was closely related to members of the genus Pseudoclavibacter and showed the highest 16S rRNA gene sequence similarities with Pseudoclavibacter helvolus KCTC 19531T (98.8 %) and Pseudoclavibacter chungangensis KCTC 22691T (96.9 %). DNA-DNA hybridization showed 41.9 ± 2.1 % and 12.4 ± 0.9 % DNA reassociation with P. helvolus KCTC 19531T and P. chungangensis KCTC 22691T, respectively. Chemotaxonomic analyses revealed that strain THG-MD12T possesses menaquinone-9 as the predominant respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan and anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0 as the major fatty acids. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and two unknown lipids. These data corroborated the affiliation of THG-MD12T to the genus Pseudoclavibacter. Thus, the isolate represents a novel species, for which the name Pseudoclavibacter terrae sp. nov. is proposed, with THG-MD12T as the type strain ( = CCTCC AB 2015124T = KCTC 39562T).
authors: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors: Juan
authors: J
authors: Du
authors: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors: Hina
authors: H
authors: Singh
authors: Graduate School of Biotechnology, Kyung Hee University, Gyeonggi-do, Republic of Korea.
authors: Jung-Eun
authors: JE
authors: Yang
authors: Department of Acupuncture Merdian Science Research Center, College of Korean Medicine, Kyung Hee University Global Campus, Republic of Korea.
authors: Chang
authors: C
authors: Shik Yin
authors: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors: MooChang
authors: M
authors: Kook
authors: College of Biotechnology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalia 116034, PR China.
authors: Hongshan
authors: H
authors: Yu
authors: Graduate School of Biotechnology, Kyung Hee University, Gyeonggi-do, Republic of Korea.|Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors: Tae-Hoo
authors: TH
authors: Yi
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000613:Aminobutyrates / Q000737:chemistry; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D031441:Ophiopogon / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000613:Aminobutyrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C005959:2,4-diaminobutyric acid; C073355:menaquinone 9