title: Separation, concentration and determination of trace chloramphenicol in shrimp from different waters by using polyoxyethylene lauryl ether-salt aqueous two-phase system coupled with high-performance liquid chromatography.
abstract: Polyoxyethylene lauryl ether (POELE10)-NaH2PO4 aqueous two-phase extraction system (ATPES) is coupled with HPLC to analyze chloramphenicol (CAP) in aquatic product. Response surface methodology (RSM) was adopted in the multi-factor experiment to determine the optimized conditions. The extraction efficiency of CAP (E%) is up to 99.42% under the optimal conditions, namely, the concentration of NaH2PO4, the concentration of POELE10, pH and temperature were 0.186 g · mL(-1), 0.033 g · mL(-1), 3.8 and 25 °C respectively. The optimal value of enrichment factor of CAP (F) was 22.56 when the concentration of NaH2PO4 was 0.192 g · mL(-1), the concentration of POELE10 was 0.024 g/ml, pH was 4.2 and temperature was 30 °C. The limit of detection (LOD) and limit of quantification (LOQ) of this method are 0.8 μg · kg(-1) and 1 μg · kg(-1), which meet the needs of determining trace or ultratrace CAP in food. The E% and F of this technique are much better than other extraction methods.
authors: School of Chemistry and Computer Science, Jilin Normal University, 1301 Haifeng Street, Siping 136000, China; School of Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors: Yang
authors: Y
authors: Lu
authors: School of Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors: Hui
authors: H
authors: Yao
authors: School of Chemistry and Computer Science, Jilin Normal University, 1301 Haifeng Street, Siping 136000, China.
authors: Chuang
authors: C
authors: Li
authors: School of Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors: Juan
authors: J
authors: Han
authors: School of Chemistry and Computer Science, Jilin Normal University, 1301 Haifeng Street, Siping 136000, China. Electronic address: zhenjiangtansp@126.com.
authors: Zhenjiang
authors: Z
authors: Tan
authors: School of Chemistry and Computer Science, Jilin Normal University, 1301 Haifeng Street, Siping 136000, China; School of Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. Electronic address: yongshengyanjs@126.com.
authors: Yongsheng
authors: Y
authors: Yan
mesh_terms: D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D033561:Penaeidae / Q000737:chemistry*; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000737:chemistry*; D045483:Rivers / Q000737:chemistry; D012623:Seawater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D000077423:Polidocanol; D011092:Polyethylene Glycols; D002701:Chloramphenicol
keywords: Aqueous two-phase system; Chloramphenicol; Determine; Polyoxyethylene lauryl ether; Trace