title: Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells.
abstract: It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.
authors: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors: Martijn A
authors: MA
authors: Langereis
authors: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors: Huib H
authors: HH
authors: Rabouw
authors: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors: Melle
authors: M
authors: Holwerda
authors: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors: Linda J
authors: LJ
authors: Visser
authors: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands F.J.M.vanKuppeveld@uu.nl.
authors: Frank J M
authors: FJ
authors: van Kuppeveld
mesh_terms: D064113:CRISPR-Cas Systems; D003384:Coxsackievirus Infections / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005434:Flow Cytometry; D055786:Gene Knockout Techniques; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008574:Mengovirus / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014162:Transfection / Q000379:methods; D000097573:Red Fluorescent Protein; D000099197:STING Protein; D000099235:Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D009713:Nucleotidyltransferases; D000097573:Red Fluorescent Protein; C532857:STING1 protein, human; D000099197:STING Protein; C485184:enhanced green fluorescent protein; C578390:cGAS protein, human; D000099235:Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase