title: Acoustofluidic, label-free separation and simultaneous concentration of rare tumor cells from white blood cells.
abstract: Enrichment of rare cells from peripheral blood has emerged as a means to enable noninvasive diagnostics and development of personalized drugs, commonly associated with a prerequisite to concentrate the enriched rare cell population prior to molecular analysis or culture. However, common concentration by centrifugation has important limitations when processing low cell numbers. Here, we report on an integrated acoustophoresis-based rare cell enrichment system combined with integrated concentration. Polystyrene 7 μm microparticles could be separated from 5 μm particles with a recovery of 99.3 ± 0.3% at a contamination of 0.1 ± 0.03%, with an overall 25.7 ± 1.7-fold concentration of the recovered 7 μm particles. At a flow rate of 100 μL/min, breast cancer cells (MCF7) spiked into red blood cell-lysed human blood were separated with an efficiency of 91.8 ± 1.0% with a contamination of 0.6 ± 0.1% from white blood cells with a 23.8 ± 1.3-fold concentration of cancer cells. The recovery of prostate cancer cells (DU145) spiked into whole blood was 84.1 ± 2.1% with 0.2 ± 0.04% contamination of white blood cells with a 9.6 ± 0.4-fold concentration of cancer cells. This simultaneous on-chip separation and concentration shows feasibility of future acoustofluidic systems for rapid label-free enrichment and molecular characterization of circulating tumor cells using peripheral venous blood in clinical practice.
authors: Department of Biomedical Engineering, Lund University , Ole Römers väg 3, 22363 Lund, Sweden.
authors: Maria
authors: M
authors: Antfolk
authors: Department of Translational Medicine, Lund University , Jan Waldenströms gata 35, 205 02 Malmö, Sweden.
authors: Cecilia
authors: C
authors: Magnusson
authors: Department of Biomedical Engineering, Lund University , Ole Römers väg 3, 22363 Lund, Sweden.|Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors: Per
authors: P
authors: Augustsson
authors: Department of Translational Medicine, Lund University , Jan Waldenströms gata 35, 205 02 Malmö, Sweden.|Department of Laboratory Medicine, Surgery (Urology), and Medicine (GU Oncology), Memorial Sloan Kettering Cancer Center , 1275 York Avenue, New York, New York 10065, United States.|Nuffield Department of Surgical Sciences, University of Oxford John Radcliffe Hospital , Headington, Oxford OX3 9DU, United Kingdom.|Institute for Biosciences and Medical Technology, University of Tampere , Biokatu 10, 33520 Tampere, Finland.
authors: Hans
authors: H
authors: Lilja
authors: Department of Biomedical Engineering, Lund University , Ole Römers väg 3, 22363 Lund, Sweden.
authors: Thomas
authors: T
authors: Laurell
mesh_terms: D000162:Acoustics; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D048429:Cell Size; D004867:Equipment Design; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011137:Polystyrenes / Q000737:chemistry; D013997:Time Factors
chemical_list: D011137:Polystyrenes