title: Determination of mutagenic amines in water and food samples by high pressure liquid chromatography with amperometric detection using a multiwall carbon nanotubes-glassy carbon electrode.
abstract: A chromatographic method, using amperometric detection, for the sensitive determination of six representative mutagenic amines was developed. A glassy carbon electrode (GCE), modified with multiwall carbon nanotubes (GCE-CNTs), was prepared and its response compared to a conventional glassy carbon electrode. The chromatographic method (HPLC-GCE-CNTs) allowed the separation and the determination of heterocyclic aromatic amines (HAAs) classified as mutagenic amines by the International Agency for Research of Cancer. The new electrode was systematically studied in terms of stability, sensitivity, and reproducibility. Statistical analysis of the obtained data demonstrated that the modified electrode provided better sensitivity than the conventional unmodified ones. Detection limits were in the 3.0 and 7.5 ng/mL range, whereas quantification limits ranged between 9.5 and 25.0 ng/mL were obtained. The applicability of the method was demonstrated by the determination of the amines in several types of samples (water and food samples). Recoveries indicate very good agreement between amounts added and those found for all HAAs (recoveries in the 92% and 105% range).
authors: Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Campus de Ciudad Real, E-13004 Ciudad Real, Spain.
authors: Ana María
authors: AM
authors: Bueno
authors: Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Campus de Ciudad Real, E-13004 Ciudad Real, Spain.
authors: Miguel Ángel
authors: MÁ
authors: Marín
authors: Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Campus de Ciudad Real, E-13004 Ciudad Real, Spain.
authors: Ana María
authors: AM
authors: Contento
authors: Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Campus de Ciudad Real, E-13004 Ciudad Real, Spain. Electronic address: angel.rios@uclm.es.
authors: Ángel
authors: Á
authors: Ríos
mesh_terms: D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004566:Electrodes; D005506:Food Contamination / Q000032:analysis*; D006571:Heterocyclic Compounds / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D009153:Mutagens / Q000032:analysis* / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D000588:Amines; D006571:Heterocyclic Compounds; D009153:Mutagens; D037742:Nanotubes, Carbon; D014874:Water Pollutants, Chemical
keywords: Amperometric detection; Liquid chromatography; Modified electrode; Multiwall carbon nanotubes; Mutagenic amines