title: Spatiotemporal drug delivery using laser-generated-focused ultrasound system.
abstract: Laser-generated-focused ultrasound (LGFU) holds promise for the high-precision ultrasound therapy owing to its tight focal spot, broad frequency band, and stable excitation with minimal ultrasound-induced heating. We here report the development of the LGFU as a stimulus for promoted drug release from microgels integrated with drug-loaded polymeric nanoparticles. The pulsed waves of ultrasound, generated by a carbon black/polydimethylsiloxane (PDMS)-photoacoustic lens, were introduced to trigger the drug release from alginate microgels encapsulated with drug-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles. We demonstrated the antibacterial capability of this drug delivery system against Escherichia coli by the disk diffusion method, and antitumor efficacy toward the HeLa cell-derived tumor spheroids in vitro. This novel LGFU-responsive drug delivery system provides a simple and remote approach to precisely control the release of therapeutics in a spatiotemporal manner and potentially suppress detrimental effects to the surrounding tissue, such as thermal ablation.
authors: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors: Jin
authors: J
authors: Di
authors: Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USA.
authors: Jinwook
authors: J
authors: Kim
authors: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors: Quanyin
authors: Q
authors: Hu
authors: Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: xjiang5@ncsu.edu.
authors: Xiaoning
authors: X
authors: Jiang
authors: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: zgu@email.unc.edu.
authors: Zhen
authors: Z
authors: Gu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000737:chemistry; D003692:Delayed-Action Preparations; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D052978:Disk Diffusion Antimicrobial Tests; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers; D004867:Equipment Design; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006367:HeLa Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D007834:Lasers; D053758:Nanoparticles; D061088:Photoacoustic Techniques; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012995:Solubility; D053260:Soot / Q000737:chemistry; D018874:Spheroids, Cellular; D013678:Technology, Pharmaceutical / Q000295:instrumentation* / Q000379:methods; D014159:Transducers; D000069453:Ultrasonic Waves; D014465:Ultrasonics / Q000295:instrumentation* / Q000379:methods
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D004129:Dimethylpolysiloxanes; D004337:Drug Carriers; D005782:Gels; D006603:Hexuronic Acids; D053260:Soot; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002939:Ciprofloxacin; D004317:Doxorubicin; D020723:Glucuronic Acid
keywords: Drug delivery; Microgels; Nanoparticles; Photoacoustic; Stimuli responsive