title: Occurrence of C. botulinum in healthy cattle and their environment following poultry botulism outbreaks in mixed farms.
abstract: Ten cattle farms located in an area with a recent history of poultry botulism outbreaks were investigated to evaluate the occurrence of toxigenic C. botulinum in healthy cattle. Environmental samples in the 10 cattle farms and bovine fecal contents in farms with a confirmed environmental contamination were collected. Detection of C. botulinum toxin genes C, D, C/D, D/C and E was performed using real-time PCR. 4.9% (7/143) of the environmental samples collected in the 10 investigated cattle farms were positive for C. botulinum type C/D. Theses samples (boot-swabs in stalls and on pasture and water of a stream) were collected in 3 different farms. One cow dung sample and 3 out of 64 fecal contents samples collected in a single farm were also positive for C. botulinum type C/D. This study demonstrates that cattle are probably indirectly contaminated via poultry botulism in the area and that they can be intermittent carrier of C. botulinum type C/D after poultry botulism outbreaks in mixed farms.
authors: ANSES, Ploufragan-Plouzané Laboratory, Avian and Rabbit Epidemiology and Welfare Unit, Ploufragan, France; Université Européenne de Bretagne, France. Electronic address: rozenn.souillard@anses.fr.
authors: R
authors: R
authors: Souillard
authors: ANSES, Ploufragan-Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: C
authors: C
authors: Le Maréchal
authors: GDS Bretagne, Ploufragan, France.
authors: F
authors: F
authors: Hollebecque
authors: ANSES, Ploufragan-Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: S
authors: S
authors: Rouxel
authors: GDS Bretagne, Ploufragan, France.
authors: A
authors: A
authors: Barbé
authors: ANSES, Ploufragan-Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: E
authors: E
authors: Houard
authors: ANSES, Ploufragan-Plouzané Laboratory, Avian and Rabbit Epidemiology and Welfare Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: D
authors: D
authors: Léon
authors: ANSES, Ploufragan-Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: T
authors: T
authors: Poëzévara
authors: ANSES, Food Safety Laboratory, Plateform Identypath, Maisons Alfort, France.
authors: P
authors: P
authors: Fach
authors: ANSES, Food Safety Laboratory, Plateform Identypath, Maisons Alfort, France.
authors: C
authors: C
authors: Woudstra
authors: GDS Bretagne, Ploufragan, France.
authors: F
authors: F
authors: Mahé
authors: ANSES, Ploufragan-Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: M
authors: M
authors: Chemaly
authors: ANSES, Ploufragan-Plouzané Laboratory, Avian and Rabbit Epidemiology and Welfare Unit, Ploufragan, France; Université Européenne de Bretagne, France.
authors: S
authors: S
authors: Le Bouquin
mesh_terms: D000818:Animals; D001906:Botulism / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D003014:Clostridium botulinum / Q000302:isolation & purification*; D004196:Disease Outbreaks / Q000662:veterinary*; D004783:Environmental Microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D011200:Poultry; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*
keywords: C. botulinum; Cattle; Healthy