title: Monitoring the contents of six steroidal and phenolic endocrine disrupting chemicals in chicken, fish and aquaculture pond water samples using pre-column derivatization and dispersive liquid-liquid microextraction with the aid of experimental design methodology.
abstract: This research established a sensitive and efficient pre-column derivatization HPLC method based on dispersive liquid-liquid microextraction (DLLME) for the simultaneous determination of six steroidal and phenolic endocrine disrupting chemicals (EDCs). In this study, EDCs were firstly labeled by the derivatization reagent 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and then extracted by DLLME. The response surface methodology was employed to investigate the key parameters of pre-column derivatization and DLLME. Under the optimal conditions, a good linear relationship between the peak area and the concentration of analytes was observed with correlation coefficients of >0.9991. Limits of detection for all EDCs derivatives were achieved within the range of 0.02-0.07 μg L(-1). The proposed method has the advantages of simple operation, low consumption of organic solvent, saving time, low output limit and good selectivity. When applied to several food and water samples analysis, it demonstrated good applicability for the determination of EDCs.
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China; Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors: Hongliang
authors: H
authors: Wu
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China. Electronic address: 61254368@163.com.
authors: Guoliang
authors: G
authors: Li
authors: College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Ocean University, Zhanjiang, People's Republic of China.
authors: Shucheng
authors: S
authors: Liu
authors: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors: Na
authors: N
authors: Hu
authors: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors: Dandan
authors: D
authors: Geng
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors: Guang
authors: G
authors: Chen
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors: Zhiwei
authors: Z
authors: Sun
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors: Xianen
authors: X
authors: Zhao
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors: Lian
authors: L
authors: Xia
authors: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China; Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China. Electronic address: jmyou6304@163.com.
authors: Jinmao
authors: J
authors: You
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D005618:Fresh Water / Q000032:analysis*; D059627:Liquid Phase Microextraction / Q000379:methods*; D010636:Phenols / Q000378:metabolism*; D061005:Ponds / Q000032:analysis*; D012997:Solvents / Q000737:chemistry
chemical_list: D052244:Endocrine Disruptors; D010636:Phenols; D012997:Solvents
keywords: Box–Behnken design; Dispersive liquid–liquid microextraction; Endocrine disrupting chemicals; High-performance liquid chromatography with fluorescence detection; Pre-column derivatization