title: Influence of Surface Charge and Polymer Coating on Internalization and Biodistribution of Polyethylene Glycol-Modified Iron Oxide Nanoparticles.
abstract: The aim of this study was to investigate the influence of the surface charge and coating of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) on their in vitro and in vivo behaviors. Neutral and negatively-charged PEG-based SPIONs were synthesized and compared to Resovist, a carboxydextran-based SPION currently used in clinics. Their cytotoxicity, cell internalization, and potential as contrast agents for magnetic resonance imaging were assessed. Neutral pegylated SPIONs were internalized less readily by the reticuloendothelial system and showed a lower uptake by the liver, compared to negatively-charged SPIONs (with carboxydextran and PEG). These results suggested that the charge of functionalized SPIONs was more relevant for their biological interactions than the nature of their coating.
authors: Lionel
authors: L
authors: Maurizi
authors: Anne-Laure
authors: AL
authors: Papa
authors: Laure
authors: L
authors: Dumont
authors: Frédéric
authors: F
authors: Bouyer
authors: Paul
authors: P
authors: Walker
authors: David
authors: D
authors: Vandroux
authors: Nadine
authors: N
authors: Millot
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000633:toxicity; D003911:Dextrans / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D056945:Hep G2 Cells; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D008422:Materials Testing; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D009928:Organ Specificity; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D055672:Static Electricity; D013329:Structure-Activity Relationship; D014018:Tissue Distribution
chemical_list: D020099:Coated Materials, Biocompatible; D003911:Dextrans; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; C097921:ferumoxtran-10; D011092:Polyethylene Glycols