title: Chemical Characterization of Fucoidan from Commercially Cultured Nemacystus decipiens (Itomozuku).
abstract: Fucoidan was isolated from Nemacystus decipiens KUCKUCK which is commercially cultured at the rate of about 2,000 t/year in fields of the Okinawa Islands, in Japan. The yield of fucoidan extracted by 0.1 M HCl was 0.5% (W/W) based on the wet algae. The total carbohydrate, sulfuric acid, ash, and moisture in fucoidan was 65.8, 30.8, 22.3, and 3.8%, respectively. Fucose and galactose were identified by liquid chromatography. The molar ratio of the sugar residues in the fucoidan was estimated to be L-fucose:D-galactose:sulfuric acid=1.0:0.05:1.0. The optical rotation of the fucoidan (0.4%) showed a value of -0.315° at 60°C, then it decreased a little with decreasing temperature. The infrared spectrum of isolated fucoidan is in agreement with that of standard fucoidan. The molecular mass of fucoidan was estimated to be 2.4×10(5). The (1)H- and (13)C-NMR spectral data suggest that fucoidan is mainly composed of L-fucopyranose, 2-mono-O-sulfo-L-fucopyranose, 4-mono-O-sulfo-L-fucopyranose, and 2,4-di-O-sulfo-L-fucopyranose.
authors: a Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus.
authors: M
authors: M
authors: Tako
authors: T
authors: T
authors: Nakada
authors: F
authors: F
authors: Hongou
mesh_terms: D017756:Aquaculture; D005643:Fucose / Q000031:analogs & derivatives / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D007564:Japan; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D013464:Sulfuric Acids; D005643:Fucose; C007789:fucoidan; C033158:sulfuric acid; D006851:Hydrochloric Acid; D005690:Galactose
keywords: Itomozuku; Nemacystus decipiens; fucoidan