title: MiR-21 inhibits autophagy by targeting Rab11a in renal ischemia/reperfusion.
abstract: Renal ischemia-reperfusion (I/R) is one of the main causes of the acute kidney injury (AKI) that usually occurs during clinical surgery. Autophagy plays an important role in recovery from acute ischemic kidney injury. MicroRNA-21 (miR-21) was reported to inhibit autophagy in several diseases. However, the molecular mechanism of miR-21 on autophagy during renal I/R is still unclear. For the in vitro study, NRK-52E cells were transfected with miR-21 mimics and subjected to I/R. Results showed that miR-21 mimics inhibited cell viability and induced cell apoptosis in NRK-52E cells. Expression of beclin-1 and LC3-II was induced, and p62 was decreased by I/R. miR-21 mimics inhibited this induction. RT-PCR and western blotting assay showed that miR-21 mimics inhibited the protein level of Rab11a, but not the mRNA level. A luciferase activity assay showed that miR-21 directly targeted Rab11a 3'-UTR. Rab11a overexpression attenuated the effect of miR-21 mimics and I/R on cell viability and cell apoptosis. The expression of beclin-1 and LC3-II was increased, and p62 was decreased by Rab11a overexpression. For the in vivo assay in a rat I/R model, the miR-21 level was increased during renal I/R injury. Pre-treatment with miR-21 inhibitor injection attenuated the renal injury, and enhanced expression of LC3-II and beclin-1. The results showed that miR-21 inhibited autophagy by targeting Rab11a in renal I/R, indicating that Rab11a might be a new medical target for the treatment of renal I/R.
authors: Department of Nephrology, the 94th Hospital of Chinese PLA, The Changcheng Affiliated Hospital of Nanchang University, Nanchang 330002, China. Electronic address: xiujuan_liu796@163.com.
authors: Xiujuan
authors: X
authors: Liu
authors: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
authors: Quan
authors: Q
authors: Hong
authors: Department of Nephrology, the 94th Hospital of Chinese PLA, The Changcheng Affiliated Hospital of Nanchang University, Nanchang 330002, China.
authors: Zhen
authors: Z
authors: Wang
authors: Department of Nephrology, the 94th Hospital of Chinese PLA, The Changcheng Affiliated Hospital of Nanchang University, Nanchang 330002, China.
authors: Yanyan
authors: Y
authors: Yu
authors: Department of Nephrology, the 94th Hospital of Chinese PLA, The Changcheng Affiliated Hospital of Nanchang University, Nanchang 330002, China.
authors: Xin
authors: X
authors: Zou
authors: Department of Nephrology, the 94th Hospital of Chinese PLA, The Changcheng Affiliated Hospital of Nanchang University, Nanchang 330002, China.
authors: Lihong
authors: L
authors: Xu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001343:Autophagy; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D007511:Ischemia / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C546082:mirn21 microRNA, rat
keywords: Autophagy; Rab11a; Renal ischemia/reperfusion; miR-21