title: Endogenous cellulolytic enzyme systems in the longhorn beetle Mesosa myops (Insecta: Coleoptera) studied by transcriptomic analysis.
abstract: The Cerambycidae (longhorn beetle) is a large family of Coleoptera with xylophagous feeding habits. Cellulose digestion plays an important role in these wood-feeding insects. In this study, transcriptomic technology was used to obtain one glycoside hydrolase family 45 (GH45) cellulase and seven GH5 cellulases from Mesosa myops, a typical longhorn beetle. Analyses of expression dynamics and evolutionary relationships provided a complete description of the cellulolytic system. The expression dynamics related to individual development indicated that endogenous GH45 and GH5 cellulases dominate cellulose digestion in M. myops. Evolutionary analyses suggested that GH45 cellulase gene is a general gene in the Coleoptera Suborder Polyphaga. Evolutionary analyses also indicated that the GH5 cellulase group in Lamiinae longhorn beetles is closely associated with wood feeding. This study demonstrated that there is a complex endogenous cellulolytic system in M. myops that is dominated by cellulases belonging to two glycoside hydrolase families.
authors: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Jie
authors: J
authors: Liu
authors: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Keqing
authors: K
authors: Song
authors: University of Chinese Academy of Sciences, Beijing 100049, China Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Huajing
authors: H
authors: Teng
authors: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Bin
authors: B
authors: Zhang
authors: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Wenzhu
authors: W
authors: Li
authors: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China yangxk@ioz.ac.cn xue@ioz.ac.cn.
authors: Huaijun
authors: H
authors: Xue
authors: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China yangxk@ioz.ac.cn xue@ioz.ac.cn.
authors: Xingke
authors: X
authors: Yang
mesh_terms: D000818:Animals; D002480:Cellulase / Q000378:metabolism*; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D019143:Evolution, Molecular; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D010802:Phylogeny; D059467:Transcriptome
chemical_list: D002480:Cellulase
keywords: cellulase; longhorn beetle; transcriptome; wood-feeding habit