title: Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi.
abstract: This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].
authors: Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Maggy T
authors: MT
authors: Sikulu
authors: Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: James
authors: J
authors: Monkman
authors: Protein Discovery Centre, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Keyur A
authors: KA
authors: Dave
authors: Protein Discovery Centre, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Marcus L
authors: ML
authors: Hastie
authors: Environmental Futures Research Institute and Griffith School of Environment, Griffith University, Brisbane, Queensland, Australia.
authors: Patricia E
authors: PE
authors: Dale
authors: Environmental Futures Research Institute and Griffith School of Environment, Griffith University, Brisbane, Queensland, Australia.
authors: Roger L
authors: RL
authors: Kitching
authors: Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania ; Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
authors: Gerry F
authors: GF
authors: Killeen
authors: Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Brian H
authors: BH
authors: Kay
authors: Protein Discovery Centre, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Jeffry J
authors: JJ
authors: Gorman
authors: Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Leon E
authors: LE
authors: Hugo