title: Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells.
abstract: Pluripotent stem cell markers can be useful for diagnostic evaluation of human tumors. The novel pluripotent marker stage-specific embryonic antigen-5 (SSEA-5) is expressed in undifferentiated human induced pluripotent cells (iPSCs), but little is known about SSEA-5 expression in other primitive tissues (e.g., human tumors). We evaluated SSEA-5 immunoreactivity patterns in human tumors, cell lines, teratomas, and iPS cells together with another pluripotent cell surface marker L1 cell adhesion molecule (L1CAM). We tested two hypotheses: (1) SSEA-5 and L1CAM would be immunoreactive and colocalized in human tumors; (2) SSEA-5 and L1CAM immunoreactivity would persist in iPSCs following retinal differentiating treatment. SSEA-5 immunofluorescence was most pronounced in primitive tumors, such as embryonal carcinoma. In tumor cell lines, SSEA-5 was highly immunoreactive in Capan-1 cells, while L1CAM was highly immunoreactive in U87MG cells. SSEA-5 and L1CAM showed colocalization in undifferentiated iPSCs, with immunopositive iPSCs remaining after 20 days of retinal differentiating treatment. This is the first demonstration of SSEA-5 immunoreactivity in human tumors and the first indication of SSEA-5 and L1CAM colocalization. SSEA-5 and L1CAM warrant further investigation as potentially useful tumor markers for histological evaluation or as markers to monitor the presence of undifferentiated cells in iPSC populations prior to therapeutic use.
authors: Center for Hearing and Deafness, University at Buffalo, 3435 Main Street, Cary 137 Buffalo, NY 14214, USA.
authors: Linda
authors: L
authors: Cassidy
authors: Center for Hearing and Deafness, University at Buffalo, 3435 Main Street, Cary 137 Buffalo, NY 14214, USA.
authors: Meerim
authors: M
authors: Choi
authors: Indiana University, Department of Biology, Indianapolis, IN 46202, USA.
authors: Jason
authors: J
authors: Meyer
authors: Mount Sinai, Genetics and Genomic Sciences, New York, NY 10029, USA.
authors: Rui
authors: R
authors: Chang
authors: Center for Hearing and Deafness, University at Buffalo, 3435 Main Street, Cary 137 Buffalo, NY 14214, USA.
authors: Gail M
authors: 0000-0002-0753-3163
authors: GM
authors: Seigel