title: Loss of Centrobin Enables Daughter Centrioles to Form Sensory Cilia in Drosophila.
abstract: Sensory cilia are organelles that convey information to the cell from the extracellular environment. In vertebrates, ciliary dysfunction results in ciliopathies that in humans comprise a wide spectrum of developmental disorders. In Drosophila, sensory cilia are found only in the neurons of type I sensory organs, but ciliary dysfunction also has dramatic consequences in this organism because it impairs the mechanosensory properties of bristles and chaetae and leads to uncoordination, a crippling condition that causes lethality shortly after eclosion. The cilium is defined by the ciliary membrane, a protrusion of the cell membrane that envelops the core structure known as the axoneme, a microtubule array that extends along the cilium from the basal body. In vertebrates, basal body function requires centriolar distal and subdistal appendages and satellites. Because these structures are acquired through centriole maturation, only mother centrioles can serve as basal bodies. Here, we show that although centriole maturity traits are lacking in Drosophila, basal body fate is reserved to mother centrioles in Drosophila type I neurons. Moreover, we show that depletion of the daughter-centriole-specific protein Centrobin (CNB) enables daughter centrioles to dock on the cell membrane and to template an ectopic axoneme that, although structurally defective, protrudes out of the cell and is enveloped by a ciliary membrane. Conversely, basal body capability is inhibited in mother centrioles modified to carry CNB. These results reveal the crucial role of CNB in regulating basal body function in Drosophila ciliated sensory organs.
authors: Department of Life Sciences, University of Siena, Via Aldo Moro 2, Siena 53100, Italy.
authors: Marco
authors: M
authors: Gottardo
authors: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, Barcelona 08028, Spain.
authors: Giulia
authors: G
authors: Pollarolo
authors: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, Barcelona 08028, Spain.
authors: Salud
authors: S
authors: Llamazares
authors: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, Barcelona 08028, Spain.
authors: Jose
authors: J
authors: Reina
authors: Department of Life Sciences, University of Siena, Via Aldo Moro 2, Siena 53100, Italy.
authors: Maria G
authors: MG
authors: Riparbelli
authors: Department of Life Sciences, University of Siena, Via Aldo Moro 2, Siena 53100, Italy.
authors: Giuliano
authors: G
authors: Callaini
authors: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, Barcelona 08028, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Passeig Lluís Companys 23, Barcelona 08010, Spain. Electronic address: gonzalez@irbbarcelona.org.
authors: Cayetano
authors: C
authors: Gonzalez
mesh_terms: D000818:Animals; D054468:Axoneme / Q000378:metabolism; D064652:Basal Bodies / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002502:Centrioles / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism*; D004330:Drosophila / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D015388:Organelles / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins