title: Functional Changes of Dendritic Cells in C6 Glioma-Bearing Rats That Underwent Combined Argon-Helium Cryotherapy and IL-12 Treatment.
abstract: The aim of this study was to explore changes in tumor tissues of glioma-bearing rats that underwent argon-helium cryoablation as well as changes in antitumor immunity before and after combined interleukin 12 treatment.
  
  
  
  Two hundred sixty Wistar rats were randomly divided into a blank control group, intravenous injection interleukin-12 group, cryotherapy group, and cryotherapy + intravenous injection group. C6 glioma cells proliferated in vitro were implanted subcutaneously on the backs of rats to establish C6 glioma-bearing animal models. Each group underwent the corresponding treatments, and morphological changes in tumor tissues were examined using hematoxylin-eosin staining. CD11c staining was examined using immunohistochemistry, and differences in dendritic cells and T-cell subsets before and after treatment were analyzed using flow cytometry.
  
  
  
  The control group showed no statistical changes in terms of tumor tissue morphology and cellular immunity, cryotherapy group, and cryotherapy + intravenous injection group, among which the count for the cryotherapy + intravenous injection group was significantly higher than those of all other groups. In the argon-helium cryotherapy group, tumor cells were damaged and dendritic cell markers were positive. The number of CD11c+ and CD86+ cells increased significantly after the operation as did the cytokine interferon-γ level (P < .01), suggesting a shift toward Th1-type immunity.
  
  
  
  Combined treatment of argon-helium cryoablation and interleukin 12 for gliomas not only effectively injured tumor tissues but also boosted immune function and increased antitumor ability. Therefore, this approach is a promising treatment measure for brain gliomas.
authors: Department of Neurosurgery, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Ming
authors: M
authors: Li
authors: Department of Tumor, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Yao
authors: Y
authors: Cui
authors: Department of Tumor, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Xiqing
authors: X
authors: Li
authors: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: Yanwu
authors: Y
authors: Guo
authors: Department of Neurosurgery, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Bin
authors: B
authors: Wang
authors: Department of Neurosurgery, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Jiadong
authors: J
authors: Zhang
authors: Department of Neurosurgery, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Jian
authors: J
authors: Xu
authors: Central Research Lab, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
authors: Shuangyin
authors: S
authors: Han
authors: Department of Neurosurgery, The People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China 13598888356@163.com.
authors: Xiwen
authors: X
authors: Shi
mesh_terms: D000818:Animals; D001128:Argon; D003131:Combined Modality Therapy; D003452:Cryosurgery; D017679:Cryotherapy; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005910:Glioma / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D006371:Helium; D007150:Immunohistochemistry; D018664:Interleukin-12 / Q000494:pharmacology*; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051381:Rats; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D016207:Cytokines; D018664:Interleukin-12; D006371:Helium; D001128:Argon
keywords: IL-12; T-cell subset; argon–helium cryosurgery; dendritic cell; glioma