title: Quantitative analysis of mebendazole polymorphs in pharmaceutical raw materials using near-infrared spectroscopy.
abstract: This work evaluates the feasibility of using NIR spectroscopy for quantification of three polymorphs of mebendazole (MBZ) in pharmaceutical raw materials. Thirty ternary mixtures of polymorphic forms of MBZ were prepared, varying the content of forms A and C from 0 to 100% (w/w), and for form B from 0 to 30% (w/w). Reflectance NIR spectra were used to develop partial least square (PLS) regression models using all spectral variables and the variables with significant regression coefficients selected by the Jack-Knife algorithm (PLS/JK). MBZ polymorphs were quantified with RMSEP values of 2.37% w/w, 1.23% w/w and 1.48% w/w for polymorphs A, B and C, respectively. This is an easy, fast and feasible method for monitoring the quality of raw pharmaceutical materials of MBZ according to polymorph purity.
authors: Departamento de Química, Universidade Federal Rural de Pernambuco-UFRPE, Rua Dom Manuel de Medeiros s/n, Dois Irmãos, 52071-030 Recife, PE, Brazil.
authors: Vitor H
authors: VH
authors: da Silva
authors: Departamento de Química Fundamental, Universidade Federal de Pernambuco-UFPE, Av. Jornalista Aníbal Fernandes, s/n, Cidade Universitária, 50740-560 Recife, PE, Brazil.
authors: Jacqueline L
authors: JL
authors: Gonçalves
authors: Departamento de Química, Universidade Federal da Paraíba-UFPB, Caixa Postal: 5093, 58051-970 João Pessoa, PB, Brazil.
authors: Fernanda V C
authors: FV
authors: Vasconcelos
authors: Departamento de Engenharia Química, Laboratório de Combustíveis (LAC), Universidade Federal de Pernambuco-UFPE, Avenida Prof. Arthur de Sá s/n, Cidade Universitária, 50740-521 Recife, PE, Brazil.
authors: M Fernanda
authors: MF
authors: Pimentel
authors: Departamento de Química Fundamental, Universidade Federal de Pernambuco-UFPE, Av. Jornalista Aníbal Fernandes, s/n, Cidade Universitária, 50740-560 Recife, PE, Brazil. Electronic address: clauimp@gmail.com.
authors: Claudete F
authors: CF
authors: Pereira
mesh_terms: D000871:Anthelmintics / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D004340:Drug Contamination; D005240:Feasibility Studies; D016018:Least-Squares Analysis; D008463:Mebendazole / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000871:Anthelmintics; D008463:Mebendazole
keywords: Mebendazole (MBZ); Near infrared spectroscopy (NIR); Partial least squares (PLS) regression; Polymorphs