title: Identification and characterization of a dipeptidyl peptidase IV inhibitor from aronia juice.
abstract: Aronia berries have many potential effects on health, including an antioxidant effect, effect for antimutagenesis, hepatoprotection and cardioprotection, an antidiabetic effect and inhibition of cancer cell proliferation. Previous human studies have shown that aronia juice may be useful for treatment of obesity disorders. In this study, we found that aronia juice has an inhibitory effect against dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5). DPP IV is a peptidase that cleaves the N-terminal region of incretins such as glucagon-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Inactivation of incretins by DPP IV induces reduction of insulin secretion. Furthermore, we identified that cyanidin 3, 5-diglucoside as the DPP IV inhibitor in aronia juice. DPP IV was inhibited more strongly by cyanidin 3, 5-diglucoside than by cyanidin and cyanidin 3-glucoside. The results suggest that DPP IV is inhibited by cyanidin 3, 5-diglucoside present in aronia juice. The antidiabetic effect of aronia juice may be mediated through DPP IV inhibition by cyanidin 3, 5-diglucoside.
authors: Department of Health and Nutrition, Faculty of Human Science, Hokkaido Bunkyo University, Eniwa 061-1449, Japan.
authors: Miyuki
authors: M
authors: Kozuka
authors: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan. Electronic address: t-yamane@pharm.hokudai.ac.jp.
authors: Takuya
authors: T
authors: Yamane
authors: Center for Research and Development Bioresources, Research Organization for University-Community Collaborations, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
authors: Yoshihisa
authors: Y
authors: Nakano
authors: Institute of Food Sciences, Nakagaki Consulting Engineer Co., Ltd, Nishi-ku, Sakai 593-8328, Japan.
authors: Takenori
authors: T
authors: Nakagaki
authors: Department of Nutrition, School of Nursing and Nutrition, Tenshi College, Higashi-ku, Sapporo 065-0013, Japan.
authors: Iwao
authors: I
authors: Ohkubo
authors: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
authors: Hiroyoshi
authors: H
authors: Ariga
mesh_terms: D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry* / Q000302:isolation & purification*; D004789:Enzyme Activation; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D046936:Photinia; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D005960:Glucosides; D010936:Plant Extracts; D018819:Dipeptidyl Peptidase 4
keywords: Antidiabetic effect; Aronia juice; Cyanidin 3, 5-diglucoside; DPP IV inhibitor