title: Facile synthesis of biocompatible gold nanoparticles fromVites vineferaand its cellular internalization against HBL-100 cells.
abstract: The remarkable health benefits of the chemical cocktails occluded withinVites vinefera(grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.
authors: Department of Medical Biochemistry, Dental College & Hospitals, Sathyabama University, Chennai, 600119 Tamil Nadu India.
authors: Kanchana
authors: K
authors: Amarnath
authors: Department of Biotechnology, Sathyabama University, Chennai, 600119 Tamil Nadu India.
authors: Nina Liza
authors: NL
authors: Mathew
authors: Department of Biotechnology, Sathyabama University, Chennai, 119 Tamil Nadu India.
authors: Jayshree
authors: J
authors: Nellore
authors: Department of Medical Biochemistry, Chettinad Hospital and Research Institute, Chettinad University, Chennai, 103 Tamil Nadu India.
authors: Chagam Reddy Venkat
authors: CR
authors: Siddarth
authors: Department of Biotechnology, Sathyabama University, Chennai, 600119 Tamil Nadu India.
authors: Jayanthi
authors: J
authors: Kumar
keywords: GSH-grape-gold nanoparticles; Grape-gold nanoparticles; Green synthesis; HBL-100 cells; Lipoic acid-grape-gold nanoparticles; Phytochemicals