title: Occurrence of genes coding for MSCRAMM and biofilm-associated protein Bap in Staphylococcus spp. isolated from bovine subclinical mastitis and relationship with somatic cell counts.
abstract: This study aimed to elucidate aspects of the epidemiology of bovine subclinical mastitis through the assessment of genes encoding MSCRAMM (microbial surface components recognizing adhesive matrix molecules - a group of adhesins) and protein Bap (implicated in biofilm formation), in coagulase-positive (CPS) and coagulase-negative (CNS) Staphylococcus isolated from subclinical mastitis. Milk samples were collected for microbiological exams, somatic cell count (SCC) and a survey of the genes coding for MSCRAMM (cna, eno, ebpS, fnbA, fnbB and fib) and biofilm-associated protein Bap (bap) in 106 Staphylococcus spp. isolates using PCR. The frequencies of occurrence of eno (82.1%), fnbA (72.6%), fib (71.7%) and bap (56.6%) were higher (P < 0.0001) compared with the other assessed genes (cna, ebpS and fnbB). The higher frequency of occurrence (P < 0.005) of the bap gene in CNS compared with CPS suggests that in these species biofilm formation is an important mechanism for the persistence of the infection. The medians of the SCCs in the samples where eno, fnbA, fib and bap genes were detected were higher compared with Staphylococcus without the assessed genes (P < 0.05) and negative samples (P < 0.01), which indicated that the presence of these MSCRAMM may be related to a higher intensity of the inflammatory process.
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil. Electronic address: eveline.zuniga@usp.br.
authors: Eveline
authors: E
authors: Zuniga
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Priscilla A
authors: PA
authors: Melville
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: André B S
authors: AB
authors: Saidenberg
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Marco A
authors: MA
authors: Laes
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Fernanda F
authors: FF
authors: Gonsales
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Sandra R S
authors: SR
authors: Salaberry
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Fabio
authors: F
authors: Gregori
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Paulo E
authors: PE
authors: Brandão
authors: Federal University of Piauí (UFPI), Campus Senador Helvídio Nunes de Barros, Rua Cícero Eduardo, S/N, Junco, CEP 64600-000, Picos, Piauí, Brazil.
authors: Franklin G B
authors: FG
authors: dos Santos
authors: Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 1374 - Ed. Biomédicas II, Sala 240, Butantã, CEP 05508-900, São Paulo, Brazil.
authors: Nilton E
authors: NE
authors: Lincopan
authors: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zoothecny, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil.
authors: Nilson R
authors: NR
authors: Benites
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000818:Animals; D058345:Asymptomatic Infections; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D002452:Cell Count; D004269:DNA, Bacterial / Q000235:genetics; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000166:cytology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C425726:Bap protein, Staphylococcus aureus; D004269:DNA, Bacterial; C509820:MSCRAMM proteins, Staphylococcus
keywords: Adhesins; Biofilm; Bovine subclinical mastitis; MSCRAMM-encoding genes; Somatic cell count; Staphylococcus