title: Downregulation of Long Noncoding RNA Meg3 Affects Insulin Synthesis and Secretion in Mouse Pancreatic Beta Cells.
abstract: Increasing evidence indicates that long noncoding RNAs (lncRNAs) are involved in diverse biological process. Mouse maternal expressed gene 3 (Meg3) is an imprinted gene and essential for development. Here, we explored the relationship between Meg3 and the function of mouse beta cells in vitro and in vivo. Real-time PCR analyses revealed that Meg3 was more abundantly expressed in Balb/c mouse islets than exocrine glands. Moreover, the expression of Meg3 in islets was decreased in T1DM (NOD female mice) and T2DM (db/db mice) models. Meg3 expression was modulated dynamically by glucose in Min6 cells and isolated mouse islets. The function role of Meg3 was investigated in Min6 cells and normal mouse by knockdown of Meg3 using small interfering RNA. After suppression of Meg3 expression in vitro, insulin synthesis and secretion were impaired and the rate of beta cells apoptosis was increased. Moreover, knockdown of Meg3 in vivo led to the impaired glucose tolerance and decreased insulin secretion, consisted with the reduction of insulin positive cells areas by immunochemistry assays. Notably, islets from Meg3 interference groups showed significant decrease of Pdx-1 and MafA expression in mRNA and protein levels. These results indicate that Meg3 may function as a new regulator of maintaining beta cells identity via affecting insulin production and cell apoptosis. J. Cell. Physiol. 231: 852-862, 2016. © 2015 Wiley Periodicals, Inc.
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.|Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, China.
authors: LiangHui
authors: L
authors: You
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
authors: Ning
authors: N
authors: Wang
authors: Department of Central Laboratory, The Second Affiliated Hospital of Southeast University, Nanjing, China.
authors: DanDan
authors: D
authors: Yin
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
authors: LinTao
authors: L
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
authors: FeiYan
authors: F
authors: Jin
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
authors: YaNan
authors: Y
authors: Zhu
authors: Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: QingXin
authors: Q
authors: Yuan
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
authors: Wei
authors: W
authors: De
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis* / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D007328:Insulin; C422882:MEG3 non-coding RNA, mouse; D062085:RNA, Long Noncoding; D005947:Glucose