title: Gum Arabic extracts protect against hepatic oxidative stress in alloxan induced diabetes in rats.
abstract: Gum Arabic (GA) from Acacia seyal and Acacia senegal is a branched-chain polysaccharide which has strong antioxidant properties, and has been used to reduce the experimental toxicity. Yet, the effects of GA on oxidative stress in type I diabetic rats have not been reported. The aim of the study was to investigate the effects of GA on oxidative stress in Alloxan induced diabetes in rats. The rats were divided into 3 groups (n=20 of each): control group, diabetic group injected with allaoxan, and diabetic group given 15% GA in drinking water for 8 weeks. Oxidative damage to liver tissue was evaluated by measurement of key hepatic enzymes, lipid peroxidation, antioxidant enzymes and expression of oxidative stress genes. Activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were significantly (P<0.05) increased in GA group compared to diabetic and control groups. Treatment of GA decreased liver malondialdehyde (MDA), and increased glutathione (GSH). In addition, GA was significantly (P<0.05) reduced the activities of key liver enzymes, including alanine transaminase (ALT) and aspartate transaminase (AST). SOD, GPx and heat shock protein 70 (HSP70) mRNA were significantly increased in GA group compared to control and diabetic groups. Liver of all diabetic rats showed marked degeneration whereas slight degeneration was observed in GA treated rats compared to control. The results suggest that GA may protect liver by modulating the expression of oxidative stress genes, and thus can improve antioxidant status.
authors: Department of Physiology and Biochemistry, Faculty of Veterinary Science, University of Nyala, P.O Box 155, Sudan. Electronic address: kareemo151@gmail.com.
authors: Abdelkareem A
authors: AA
authors: Ahmed
authors: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Department of Biology, Faculty of Education, University of Nyala, Nyala, Sudan.
authors: Jaafar S
authors: JS
authors: Fedail
authors: Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
authors: Hassan H
authors: HH
authors: Musa
authors: Department of Veterinary Microbiology Faculty of Animal Husbandry and Veterinary Science Sindh Agriculture University, 70060 Tandojam, Pakistan.
authors: Asghar Ali
authors: AA
authors: Kamboh
authors: Department of Physiology and Biochemistry, Faculty of Veterinary Science, University of Nyala, P.O Box 155, Sudan.
authors: Amal Z
authors: AZ
authors: Sifaldin
authors: Key Laboratory of Environmental Medicine, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China.
authors: Taha H
authors: TH
authors: Musa
keywords: Diabetes type I; Gum Arabic; Oxidative stress; Rat