title: A highly selective mitochondria-targeting fluorescent K(+) sensor.
abstract: Regulation of intracellular potassium (K(+) ) concentration plays a key role in metabolic processes. So far, only a few intracellular K(+) sensors have been developed. The highly selective fluorescent K(+) sensor KS6 for monitoring K(+) ion dynamics in mitochondria was produced by coupling triphenylphosphonium, borondipyrromethene (BODIPY), and triazacryptand (TAC). KS6 shows a good response to K(+) in the range 30-500 mM, a large dynamic range (Fmax /F0 ≈130), high brightness (ϕf =14.4 % at 150 mM of K(+) ), and insensitivity to both pH in the range 5.5-9.0 and other metal ions under physiological conditions. Colocalization tests of KS6 with MitoTracker Green confirmed its predominant localization in the mitochondria of HeLa and U87MG cells. K(+) efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin. KS6 is thus a highly selective semiquantitative K(+) sensor suitable for the study of mitochondrial potassium flux in live cells.
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA).
authors: Xiangxing
authors: X
authors: Kong
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA).
authors: Fengyu
authors: F
authors: Su
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA).
authors: Liqiang
authors: L
authors: Zhang
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA).
authors: Jordan
authors: J
authors: Yaron
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA).
authors: Fred
authors: F
authors: Lee
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA).
authors: Zhengwei
authors: Z
authors: Shi
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA). yanqing.tian@asu.edu.|Department of Materials Science and Engineering, South University of Science and Technology of China, No. 1088, Xueyuan Rd., Xili, Nanshan District, Shenzhen, Guangdong, 518055 (China). yanqing.tian@asu.edu.
authors: Yanqing
authors: Y
authors: Tian
authors: Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, 1001 S. McAlister Ave., P.O. Box 876501, Tempe, AZ 85287 (USA). deirdre.meldrum@asu.edu.
authors: Deirdre R
authors: DR
authors: Meldrum
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D002414:Cations, Monovalent / Q000032:analysis / Q000378:metabolism; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods; D011188:Potassium / Q000032:analysis* / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002414:Cations, Monovalent; D005456:Fluorescent Dyes; D015221:Potassium Channels; D011188:Potassium
keywords: BODIPY; fluorescent probes; ion channels; mitochondria; potassium