title: Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.
abstract: The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.
authors: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
authors: Po-Lin
authors: PL
authors: Chiu
authors: The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors: Xueming
authors: X
authors: Li
authors: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; The Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
authors: Zongli
authors: Z
authors: Li
authors: Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, MA 02454, USA.
authors: Brian
authors: B
authors: Beckett
authors: Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, MA 02454, USA.
authors: Axel F
authors: AF
authors: Brilot
authors: Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors: Nikolaus
authors: N
authors: Grigorieff
authors: The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA; The Howard Hughes Medical Institute, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors: David A
authors: DA
authors: Agard
authors: The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA. Electronic address: ycheng@ucsf.edu.
authors: Yifan
authors: Y
authors: Cheng
authors: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; The Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. Electronic address: twalz@hms.harvard.edu.
authors: Thomas
authors: T
authors: Walz
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000737:chemistry*; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004583:Electrons; D005136:Eye Proteins / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007903:Lens Capsule, Crystalline; D057230:Limit of Detection; D008565:Membrane Proteins / Q000032:analysis*; D012756:Sheep
chemical_list: D020346:Aquaporins; D005136:Eye Proteins; D008565:Membrane Proteins; C030328:aquaporin 0
keywords: Direct electron detection device camera; Electron counting; Super-resolution; Two-dimensional crystal