title: Urinary Klotho measured by ELISA as an early biomarker of acute kidney injury in patients after cardiac surgery or coronary angiography.
abstract: BACKGROUND
  Acute kidney injury (AKI) is a common complication after cardiac surgery and percutaneous coronary interventions which markedly worsens prognosis. In recent years, new early biomarkers of AKI have been identified, but many important aspects still remain to be solved. Klotho is a pleiotropic protein that acts as a paracrine and endocrine factor in multiple organs. Reduced renal Klotho levels have been show in several animal models of AKI. No study has been published in which Klotho was tested in humans as an early marker of AKI. The aim of this work is to assess the usefulness of measuring urinary Klotho for the early diagnosis of AKI in patients with acute coronary syndrome or heart failure undergoing cardiac surgery or coronary angiography.
  
  
  METHODS
  Urinary Klotho was measured 12 hours after intervention in 60 patients admitted to the Intensive Care Unit with acute coronary syndrome or heart failure secondary to coronary or valvular conditions, who underwent coronary angiography (30 patients) or cardiac bypass surgery or heart valve replacement (30 patients). The primary endpoint used was the onset of AKI according to the RIFLE classification system. Human Klotho levels were measured using an ELISA assay.
  
  
  RESULTS
  We found no differences in urinary Klotho levels between AKI patients and those who did not develop AKI. Moreover, there was not significant correlation between urinary Klotho levels and the presence of AKI.
  
  
  CONCLUSIONS
  Urinary Klotho measured by ELISA does not seem to be a good candidate to be used as an early biomarker of AKI.
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España; Departamento de Medicina. Universidad de Valencia. Valencia, España. Electronic address: isist67@gmail.com.
authors: Isidro
authors: I
authors: Torregrosa
authors: Fundación Investigación Hospital Clínico de Valencia. Instituto de Investigación Sanitaria - INCLIVA. Valencia, España; Departamento de Patología. Sección Histología. Facultad de Medicina y Odontología. Universidad de Valencia. Valencia, España.
authors: Carmina
authors: C
authors: Montoliu
authors: Fundación Investigación Hospital Clínico de Valencia. Instituto de Investigación Sanitaria - INCLIVA. Valencia, España.
authors: Amparo
authors: A
authors: Urios
authors: Laboratorio de Neurobiología. Centro Investigación Príncipe Felipe. Valencia, España.
authors: Carla
authors: C
authors: Giménez-Garzó
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España.
authors: Patricia
authors: P
authors: Tomás
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España.
authors: Miguel Ángel
authors: MÁ
authors: Solís
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España.
authors: Carmen
authors: C
authors: Ramos
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España.
authors: Isabel
authors: I
authors: Juan
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España; Departamento de Medicina. Universidad de Valencia. Valencia, España.
authors: María Jesús
authors: MJ
authors: Puchades
authors: Departamento de Bioquímica y Biología Molecular. Facultad de Medicina y Odontología - INCLIVA. Servicio de Análisis Clínicos. Hospital Universitario Dr. Peset. Universidad de Valencia. Valencia, España.
authors: Guillermo
authors: G
authors: Saez
authors: Unidad Coronaria. Hospital Clínico Universitario de Valencia. Valencia, España.
authors: María Luisa
authors: ML
authors: Blasco
authors: Servicio de Nefrología. Hospital Clínico Universitario de Valencia. Valencia, España; Departamento de Medicina. Universidad de Valencia. Valencia, España.
authors: Alfonso
authors: A
authors: Miguel
mesh_terms: D054058:Acute Coronary Syndrome / Q000000981:diagnostic imaging / Q000601:surgery / Q000652:urine; D058186:Acute Kidney Injury / Q000209:etiology / Q000652:urine*; D000368:Aged; D015415:Biomarkers; D017023:Coronary Angiography / Q000009:adverse effects; D001026:Coronary Artery Bypass; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005966:Glucuronidase / Q000652:urine*; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D000090265:Klotho Proteins; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000209:etiology / Q000652:urine*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D005966:Glucuronidase; D000090265:Klotho Proteins
keywords: Acute kidney injury; Angiografía coronaria; Biomarcadores; Biomarkers; Cardiac surgery; Cirugía cardiaca; Coronary angiography; ELISA; Fracaso Renal Agudo; Klotho