title: Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.
abstract: The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.
authors: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.
authors: Lipsy
authors: L
authors: Chopra
authors: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.
authors: Gurdeep
authors: G
authors: Singh
authors: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.
authors: Kautilya
authors: K
authors: Kumar Jena
authors: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.
authors: Debendra K
authors: DK
authors: Sahoo
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D005434:Flow Cytometry; D005520:Food Preservatives / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D018551:Solanum lycopersicum; D008460:Meat; D010539:Permeability / Q000187:drug effects; D020959:Polyethylene / Q000494:pharmacology; D011309:Preservation, Biological; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D001430:Bacteriocins; D005520:Food Preservatives; C000610567:sonorensin protein, Bacillus sonorensis; D020959:Polyethylene