title: Evaluating the Effect of Expressing a Peanut Resveratrol Synthase Gene in Rice.
abstract: Resveratrol (Res) is a type of natural plant stilbenes and phytoalexins that only exists in a few plant species. Studies have shown that the Res could be biosynthesized and accumulated within plants, once the complete metabolic pathway and related enzymes, such as the key enzyme resveratrol synthase (RS), existed. In this study, a RS gene named PNRS1 was cloned from the peanut, and the activity was confirmed in E. coli. Using transgenic approach, the PNRS1 transgenic rice was obtained. In T3 generation, the Res production and accumulation were further detected by HPLC. Our data revealed that compared to the wild type rice which trans-resveratrol was undetectable, in transgenic rice, the trans-resveratrol could be synthesized and achieved up to 0.697 μg/g FW in seedlings and 3.053 μg/g DW in seeds. Furthermore, the concentration of trans-resveratrol in transgenic rice seedlings could be induced up to eight or four-fold higher by ultraviolet (UV-C) or dark, respectively. Simultaneously, the endogenous increased of Res also showed the advantages in protecting the host plant from UV-C caused damage or dark-induced senescence. Our data indicated that Res was involved in host-defense responses against environmental stresses in transgenic rice. Here the results describes the processes of a peanut resveratrol synthase gene transformed into rice, and the detection of trans-resveratrol in transgenic rice, and the role of trans-resveratrol as a phytoalexin in transgenic rice when treated by UV-C and dark. These findings present new outcomes of transgenic approaches for functional genes and their corresponding physiological functions, and shed some light on broadening available resources of Res, nutritional improvement of crops, and new variety cultivation by genetic engineering.
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China; Department of Life Science, Qingdao Agricultural University, Tsingtao, Shandong, People's Republic of China.
authors: Shigang
authors: S
authors: Zheng
authors: Department of Agricultural Quality Standards and Testing Technology, Shandong Academy of Agricultural Sciences, Jinan, Shandong, People's Republic of China.
authors: Shanchang
authors: S
authors: Zhao
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China.
authors: Zhen
authors: Z
authors: Li
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China.
authors: Qingguo
authors: Q
authors: Wang
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China.
authors: Fangyin
authors: F
authors: Yao
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China.
authors: Lianqun
authors: L
authors: Yang
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China.
authors: Jiaowen
authors: J
authors: Pan
authors: Department of Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Department of Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan, Shandong, People's Republic of China.
authors: Wei
authors: W
authors: Liu
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis* / Q000235:genetics; D010367:Arachis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D000077185:Resveratrol; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D013267:Stilbenes / Q000378:metabolism*
chemical_list: D013267:Stilbenes; D000217:Acyltransferases; C055200:resveratrol synthase; D000077185:Resveratrol