title: Prospection and Evaluation of (Hemi) Cellulolytic Enzymes Using Untreated and Pretreated Biomasses in Two Argentinean Native Termites.
abstract: Saccharum officinarum bagasse (common name: sugarcane bagasse) and Pennisetum purpureum (also known as Napier grass) are among the most promising feedstocks for bioethanol production in Argentina and Brazil. In this study, both biomasses were assessed before and after acid pretreatment and following hydrolysis with Nasutitermes aquilinus and Cortaritermes fulviceps termite gut digestome. The chemical composition analysis of the biomasses after diluted acid pretreatment showed that the hemicellulose fraction was partially removed. The (hemi) cellulolytic activities were evaluated in bacterial culture supernatants of termite gut homogenates grown in treated and untreated biomasses. In all cases, we detected significantly higher endoglucanase and xylanase activities using pretreated biomasses compared to untreated biomasses, carboxymethylcellulose and xylan. Several protein bands with (hemi) cellulolytic activity were detected in zymograms and two-dimensional gel electrophoresis. Some proteins of these bands or spots were identified as xylanolytic peptides by mass spectrometry. Finally, the diversity of cultured cellulolytic bacterial endosymbionts associated to both Argentinean native termite species was analyzed. This study describes, for the first time, bacterial endosymbionts and endogenous (hemi) cellulases of two Argentinean native termites as well as their potential application in degradation of lignocellulosic biomass for bioethanol production.
authors: Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, (1686) Hurlingham, Provincia de Buenos Aires, Argentina.
authors: Emiliano
authors: E
authors: Ben Guerrero
authors: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Instituto de Microbiología y Zoología Agrícola, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, (1686) Hurlingham, Provincia de Buenos Aires, Argentina.
authors: Joel
authors: J
authors: Arneodo
authors: Embrapa Agroenergia, Parque Estação Biológica s/n, Av. w3 Norte (final), CEP 70770-901, Brasília, DF, Brazil.
authors: Raquel
authors: R
authors: Bombarda Campanha
authors: Embrapa Agroenergia, Parque Estação Biológica s/n, Av. w3 Norte (final), CEP 70770-901, Brasília, DF, Brazil.
authors: Patrícia
authors: P
authors: Abrão de Oliveira
authors: Laboratório Multiusuários Centralizado de Genômica Funcional Aplicada à Agropecuária e Agroenergia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Av. Pádua Dias 11, CP 83, 13400-970, Piracicaba, SP, Brazil.
authors: Mônica T
authors: MT
authors: Veneziano Labate
authors: Laboratório Multiusuários Centralizado de Genômica Funcional Aplicada à Agropecuária e Agroenergia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Av. Pádua Dias 11, CP 83, 13400-970, Piracicaba, SP, Brazil.
authors: Thaís
authors: T
authors: Regiani Cataldi
authors: Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, (1686) Hurlingham, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors: Eleonora
authors: E
authors: Campos
authors: Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, (1686) Hurlingham, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors: Angel
authors: A
authors: Cataldi
authors: Laboratório Multiusuários Centralizado de Genômica Funcional Aplicada à Agropecuária e Agroenergia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Av. Pádua Dias 11, CP 83, 13400-970, Piracicaba, SP, Brazil.
authors: Carlos A
authors: CA
authors: Labate
authors: Embrapa Agroenergia, Parque Estação Biológica s/n, Av. w3 Norte (final), CEP 70770-901, Brasília, DF, Brazil.
authors: Clenilson
authors: C
authors: Martins Rodrigues
authors: Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, (1686) Hurlingham, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors: Paola
authors: P
authors: Talia
mesh_terms: D000143:Acids / Q000737:chemistry*; D000818:Animals; D001118:Argentina; D018533:Biomass; D044602:Cellulases / Q000737:chemistry / Q000378:metabolism*; D004064:Digestive System / Q000201:enzymology / Q000382:microbiology; D019476:Insect Proteins / Q000378:metabolism; D020049:Isoptera / Q000145:classification / Q000201:enzymology* / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D031782:Pennisetum / Q000201:enzymology* / Q000382:microbiology; D011134:Polysaccharides / Q000378:metabolism*; D031786:Saccharum / Q000201:enzymology* / Q000382:microbiology; D013559:Symbiosis
chemical_list: D000143:Acids; D019476:Insect Proteins; D011134:Polysaccharides; C007916:hemicellulose; D044602:Cellulases