title: Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.
abstract: Enterovirus 71 (EV71), a member of Picornaviridae, causes severe neurological and systemic illness in children. To better understand the virus-host cell interactions, we performed a triple-SILAC-based quantitative proteomics study monitoring host cell proteome changes after EV71 infection. Based on the quantitative data for more than 4100 proteins, ∼17% of the proteins were found as significantly changed (p<0.01) at either 8 or 20 hours post infection. Five biological processes and seven protein classes showed significant differences. Functional screening of nine regulated proteins discovered the regulatory role of CHCH2, a mitochondrial protein known as a transcriptional activator for cytochrome c oxidase, in EV71 replication. Further studies showed that CHCH2 served as a negative regulator of innate immune responses. All MS data have been deposited in the ProteomeXchange with identifier PXD002483 (http://proteomecentral.proteomexchange.org/dataset/PXD002483).
authors: State Key Laboratory of Virology, Wuhan University, Wuhan, P. R. China.|College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors: Hao-Yu
authors: HY
authors: Li
authors: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors: Lei-Ke
authors: LK
authors: Zhang
authors: State Key Laboratory of Virology, Wuhan University, Wuhan, P. R. China.|College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors: Xiu-Juan
authors: XJ
authors: Zhu
authors: State Key Laboratory of Virology, Wuhan University, Wuhan, P. R. China.|College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors: Jun
authors: J
authors: Shang
authors: Wuhan Institute of Biotechnology, Wuhan, P. R. China.
authors: Xi
authors: X
authors: Chen
authors: State Key Laboratory of Virology, Wuhan University, Wuhan, P. R. China.|College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors: Ying
authors: Y
authors: Zhu
authors: State Key Laboratory of Virology, Wuhan University, Wuhan, P. R. China.|College of Life Sciences, Wuhan University, Wuhan, P. R. China.
authors: Lin
authors: L
authors: Guo
mesh_terms: D002460:Cell Line; D018450:Disease Progression; D029821:Enterovirus A, Human / Q000276:immunology / Q000502:physiology*; D004769:Enterovirus Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D020543:Proteome / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: CHCH2; Cell biology; Enterovirus 71; Picornaviridae; Triple SILAC