title: Antileishmanial activity of compounds produced by endophytic fungi derived from medicinal plant Vernonia polyanthes and their potential as source of bioactive substances.
abstract: The purpose of this work was to evaluate the antileishmanial activity of endophytic fungi isolated from leaves of Vernonia polyanthes plant and their prospective use in the discovery of bioactive compounds. Sixteen endophytes were isolated by using potato dextrose agar medium and submitted to cultivation in rice medium. The fungal cultures were extracted with ethanol and used as crude extracts for testing their antileishmanial activity. The most active ethanol extract was obtained from P2-F3 strain, which was identified as Cochliobolus sativus by ITS rRNA gene sequence data. Followed by a bioassay-guided fractionation, the cochlioquinone A, isocochlioquinone A and anhydrocochlioquinone A compounds were isolated from the crude extracts and demonstrated to inhibit the parasites. From the present work, it is possible to conclude that endophytic fungi derived from medicinal plant V. polyanthes may be considered promising source for the discovery of bioactive compounds.
authors: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, CEP 14040-901, Ribeirão Prêto, SP, Brazil.
authors: Adriana M
authors: AM
authors: do Nascimento
authors: Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus Universitário Morro do Cruzeiro, Bauxita, CEP 35400-000, Ouro Prêto, MG, Brazil.
authors: Mateus Gonçalves
authors: MG
authors: Soares
authors: Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus Universitário Morro do Cruzeiro, Bauxita, CEP 35400-000, Ouro Prêto, MG, Brazil.
authors: Fernanda K V
authors: FK
authors: da Silva Torchelsen
authors: Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus Universitário Morro do Cruzeiro, Bauxita, CEP 35400-000, Ouro Prêto, MG, Brazil.
authors: Jorge A Viana
authors: JA
authors: de Araujo
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina e Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Paula S
authors: PS
authors: Lage
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina e Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Mariana C
authors: MC
authors: Duarte
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina e Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Pedro H R
authors: PH
authors: Andrade
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina e Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Tatiana G
authors: TG
authors: Ribeiro
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina e Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Eduardo A F
authors: EA
authors: Coelho
authors: Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus Universitário Morro do Cruzeiro, Bauxita, CEP 35400-000, Ouro Prêto, MG, Brazil. andnascimen@yahoo.com.br.
authors: Andréa M
authors: AM
authors: do Nascimento
mesh_terms: D001203:Ascomycota / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D060026:Endophytes / Q000737:chemistry / Q000145:classification / Q000235:genetics; D000431:Ethanol / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007891:Leishmania / Q000187:drug effects*; D018515:Plant Leaves / Q000382:microbiology; D010946:Plants, Medicinal / Q000382:microbiology; D012335:RNA, Ribosomal / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods; D014344:Trypanocidal Agents / Q000737:chemistry / Q000494:pharmacology*; D035741:Vernonia / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D014344:Trypanocidal Agents; D000431:Ethanol
keywords: Antileishmanial activity; Bioprospection; Endophytes; Leishmania amazonensis; Vernonia polyanthes