title: A dual optical and nuclear imaging reagent for peptide labelling via disulfide bridging.
abstract: We report a concise approach to a multimodal imaging reagent for peptide labelling via disulfide bridging. The reagent is constructed using a one pot, three component, [3 + 2] cycloaddition of a fluorescent azide with a dithiomaleimide-alkyne, with concomitant incorporation of (125)I. The dithiomaleimide handle then enables site selective conjugation to a disulfide bond of a peptide whilst retaining the key structural bridging functionality, as exemplified on the therapeutic peptide octreotide.
authors: Department of Chemistry, University College London, 20 Gordon St, London, UK. j.r.baker@ucl.ac.uk.
authors: Sally A
authors: SA
authors: Fletcher
authors: Pak Kwan Brian
authors: PK
authors: Sin
authors: Muriel
authors: M
authors: Nobles
authors: Erik
authors: E
authors: Årstad
authors: Andrew
authors: A
authors: Tinker
authors: James R
authors: JR
authors: Baker
mesh_terms: D061565:Cycloaddition Reaction; D004220:Disulfides / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D064847:Multimodal Imaging; D009683:Nuclear Medicine / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D004220:Disulfides; D010455:Peptides