title: Characterization of a Self-Assembled Monolayer of 1-Thio-β-D-Glucose with Electrochemical Surface Enhanced Raman Spectroscopy Using a Nanoparticle Modified Gold Electrode.
abstract: Preparation of a nanoparticle modified gold substrate designed for characterization of hydrophilic self-assembled monolayers (SAMs) of 1-thio-β-D-glucose (TG) with electrochemical surface-enhanced Raman spectroscopy (EC-SERS) is presented. Citrate stabilized gold nanoparticles were deposited on a polycrystalline gold electrode and subjected to an electrochemical desorption procedure to completely remove all traces of adsorbed citrate. Complete desorption of citrate was confirmed by recording cyclic voltammetry curves and SERS spectra. The citrate-free nanoparticle modified gold electrode was then incubated in a 1 mg mL(-1) aqueous solution of TG for 16 h prior to being characterized by EC-SERS. The SERS spectra confirmed that at potentials more negative than -0.10 V vs SCE thioglucose forms a monolayer in which the majority of the molecules preserve their lactol ring structure and only a small fraction of molecules appear to be oxidized. At potentials more positive than -0.10 V, the oxidation of TG molecules becomes prominent, and at potentials more positive than 0.20 V vs SCE, the monolayer of TG consists chiefly of oxidized product. The SERS spectra collected in the double layer region suggest the SAM of TG is well hydrated and hence can be used for hydrophilic modifications of a gold surface.
authors: Department of Chemistry, University of Guelph , Guelph ON, Canada N1G 2W1.
authors: Scott R
authors: SR
authors: Smith
authors: Department of Chemistry, University of Guelph , Guelph ON, Canada N1G 2W1.
authors: Ryan
authors: R
authors: Seenath
authors: Department of Chemistry, University of Guelph , Guelph ON, Canada N1G 2W1.
authors: Monika R
authors: MR
authors: Kulak
authors: Department of Chemistry, University of Guelph , Guelph ON, Canada N1G 2W1.
authors: Jacek
authors: J
authors: Lipkowski
mesh_terms: D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000031:analogs & derivatives* / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D006046:Gold; C018399:1-thioglucose; D005947:Glucose