title: A novel electrochemical immunosensor for highly sensitive detection of aflatoxin B1 in corn using single-walled carbon nanotubes/chitosan.
abstract: A sensitive electrochemical immunosensor for aflatoxin B1 (AFB1) detection based on single-walled carbon nanotubes/chitosan was presented. The immunosensor was based on an indirect competitive binding to a fixed amount of anti-AFB1 between free AFB1 and AFB1-bovine serum albumin, which conjugate immobilized on covalently functionalized nanotubes/chitosan laid on the glass carbon electrode. Then, the anti-mouse immunoglobulin G secondary antibody labeled with alkaline phosphatase was bound to the electrode surface through reacting with primary antibody. Finally, alkaline phosphatase catalyzes the hydrolysis of the substrate α-naphthyl phosphate, which produced electrochemical signal. Compared with conventional methods, the established immunosensor was more sensitive and simple. Under optimal conditions, this method could quantitatively detect AFB1 from 0.01 to 100 ng mL(-1) with a detection limit of 3.5 pg mL(-1). Moreover, the immunosensor was successfully applied to assay AFB1 in corn powder, which showed good correlation with the results obtained from high performance liquid chromatography.
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Xian
authors: X
authors: Zhang
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Chao-Rui
authors: CR
authors: Li
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Wei-Cheng
authors: WC
authors: Wang
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Jian
authors: J
authors: Xue
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Ya-Ling
authors: YL
authors: Huang
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Xian-Xian
authors: XX
authors: Yang
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Bin
authors: B
authors: Tan
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
authors: Xi-Peng
authors: XP
authors: Zhou
authors: Department of Inspection, University-Town Hospital of Chongqing Medical University, No. 55 University City Road, Shapingba District, Chongqing 400016, China.
authors: Chuang
authors: C
authors: Shao
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors: Shi-Jia
authors: SJ
authors: Ding
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China. Electronic address: jfuqiu@163.com.
authors: Jing-Fu
authors: JF
authors: Qiu
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D051379:Mice; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009281:Naphthalenes; D009943:Organophosphorus Compounds; D012709:Serum Albumin / Q000032:analysis*; D013499:Surface Properties; D003313:Zea mays / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D009281:Naphthalenes; D009943:Organophosphorus Compounds; D012709:Serum Albumin; C052669:aflatoxin B1-bovine serum albumin; C014694:naphthyl phosphate; D048271:Chitosan; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Corn powder; Electrochemical immunosensor; Sensitive detection; Single-walled carbon nanotubes/chitosan