title: Genetic Diversity and mRNA Expression of Porcine MHC Class I Chain-Related 2 (SLA-MIC2) Gene and Development of a High-Resolution Typing Method.
abstract: The genetic structure and function of MHC class I chain-related (MIC) genes in the pig genome have not been well characterized, and show discordance in available data. Therefore, we have experimentally characterized the exon-intron structure and functional copy expression pattern of the pig MIC gene, SLA-MIC2. We have also studied the genetic diversity of SLA-MIC2 from seven different breeds using a high-resolution genomic sequence-based typing (GSBT) method. Our results showed that the SLA-MIC2 gene has a similar molecular organization as the human and cattle orthologs, and is expressed in only a few tissues including the small intestine, lung, and heart. A total of fifteen SLA-MIC2 alleles were identified from typing 145 animals, ten of which were previously unreported. Our analysis showed that the previously reported and tentatively named SLA-MIC2*05, 07, and 01 alleles occurred most frequently. The observed heterozygosity varied from 0.26 to 0.73 among breeds. The number of alleles of the SLA-MIC2 gene in pigs is somewhat lower compared to the number of alleles of the porcine MHC class I and II genes; however, the level of heterozygosity was similar. Our results indicate the comprehensiveness of using genomic DNA-based typing for the systemic study of the SLA-MIC2 gene. The method developed for this study, as well as the detailed information that was obtained, could serve as fundamental tools for understanding the influence of the SLA-MIC2 gene on porcine immune responses.
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Hailu
authors: H
authors: Dadi
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: MinhThong
authors: M
authors: Le
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Hunduma
authors: H
authors: Dinka
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: DinhTruong
authors: D
authors: Nguyen
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Hojun
authors: H
authors: Choi
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Hyesun
authors: H
authors: Cho
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Minkyeung
authors: M
authors: Choi
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Jin-Hoi
authors: JH
authors: Kim
authors: Animal Biotechnology Division, National Institute of Animal Science, Suwon, 441-350, Korea.
authors: Jin-Ki
authors: JK
authors: Park
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Nagasundarapandian
authors: N
authors: Soundrarajan
authors: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.
authors: Chankyu
authors: C
authors: Park
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle; D005805:Genes, MHC Class I / Q000235:genetics* / Q000276:immunology; D014644:Genetic Variation; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034421:Sus scrofa; D013552:Swine
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; C438668:swine leukocyte antigen