title: Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting.
abstract: Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250, and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.
authors: Environmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, P. R. China.|KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, P. R. China.
authors: Gen
authors: G
authors: Zhang
authors: KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, P. R. China.
authors: Yue-Him
authors: YH
authors: Wong
authors: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, P. R. China.
authors: Yu
authors: Y
authors: Zhang
authors: Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Science, Sanya City, Hainan Province, P. R. China.
authors: Li-Sheng
authors: LS
authors: He
authors: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, P. R. China.
authors: Ying
authors: Y
authors: Xu
authors: Environmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, P. R. China.|KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, P. R. China.
authors: Pei-Yuan
authors: PY
authors: Qian
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D008124:Locomotion; D018978:Molting / Q000502:physiology*; D009132:Muscles / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D020543:Proteome / Q000378:metabolism; D001468:Thoracica / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D020030:Nitric Oxide Donors; D020543:Proteome; D009599:Nitroprusside; D009569:Nitric Oxide
keywords: Animal proteomics; Barnacle; Label-free quantification; MS; Nitric oxide