title: Elevated expression of NLRP3 in patients with immune thrombocytopenia.
abstract: Immune thrombocytopenia (ITP) is a heterogeneous autoimmune disease, which is characterized by dysregulation of T cell-mediated autoimmunity. NLRP3, a largest and mostly well-studied inflammasome, has been shown to be important in the regulation of adaptive immune response, especially in T cell response. Given the closely association of imbalance of T cell response with ITP, whether NLRP3 is involved in the pathogenesis of ITP remains poorly understood. In this study, 69 active ITP patients, 21 ITP in remission and 24 age- and gender-matched healthy controls were included. Peripheral blood mononuclear cells (PBMCs) were isolated from ITP and control for isolation of RNA and plasma, which were used to measure mRNA level of NLRP3 and adaptor protein ASC by quantitative real-time PCR and IL-18 plasma level by ELISA. Meanwhile, protein was also extracted from PBMCs for Western blot analysis of NLRP3 expression. Our results showed a significantly higher expression of NLRP3, ASC and plasma IL-18 level in patients with active ITP when compared to control. The expression of NLRP3, ASC and plasma IL-18 level was significantly lower in patients in remission than that in active ITP, and no difference was observed when compared to control. Furthermore, a significantly positive correlation of NLRP3 with ASC was observed in patients with active ITP. In conclusion, increased expression of NLRP3 was associated with the pathogenesis of ITP and therapeutically targeting it might be a new strategy in the treatment of ITP.
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu Province, China.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors: Jianlin
authors: J
authors: Qiao
authors: Department of Clinical Laboratory, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
authors: Yun
authors: Y
authors: Liu
authors: Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors: Xiaoqian
authors: X
authors: Li
authors: Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors: Yuan
authors: Y
authors: Xia
authors: Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China.
authors: Yulu
authors: Y
authors: Wu
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu Province, China.
authors: Depeng
authors: D
authors: Li
authors: Department of Clinical Laboratory, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
authors: Hongchun
authors: H
authors: Li
authors: Department of Clinical Laboratory, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
authors: Ping
authors: P
authors: Ma
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu Province, China.
authors: Feng
authors: F
authors: Zhu
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu Province, China.
authors: Zhenyu
authors: Z
authors: Li
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu Province, China. lihmd@163.com.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China. lihmd@163.com.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China. lihmd@163.com.
authors: Kailin
authors: K
authors: Xu
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu Province, China. zengly2000@163.com.|Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China. zengly2000@163.com.|Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China. zengly2000@163.com.
authors: Lingyu
authors: L
authors: Zeng
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053468:CARD Signaling Adaptor Proteins; D016022:Case-Control Studies; D020170:Caspase 1 / Q000378:metabolism; D002648:Child; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000097:blood / Q000235:genetics*; D010976:Platelet Count; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D053468:CARD Signaling Adaptor Proteins; D003598:Cytoskeletal Proteins; D020382:Interleukin-18; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C402202:PYCARD protein, human; D020170:Caspase 1
keywords: ASC; IL-18; Immune thrombocytopenia; NLRP3