title: Two new triterpenoids from the seeds of blackberry (Rubus fructicosus).
abstract: Two new ursane-type triterpenoids (1, 2) attached to isopropylidenedioxy group were isolated from the seeds of blackberry (Rubus fructicosus L., Rosaceae) along with two known ursane-type triterpenoids, 2,3-O-isopropylidenyl-2α,3α,19α-trihydroxyurs-12-en-28-oic acid (3) and 1β-hydroxyeuscaphic acid (4). The chemical structures of 1 and 2 were determined to be 2,3-O-isopropylidene-1β,2β,3β,19α-tetrahydroxyurs-12-en-28-oic acid and 1,2-O-isopropylidene-1β,2α,3α,19α-tetrahydroxyurs-12-en-28-oic acid, respectively, based on spectroscopic data. Additionally, their cytotoxic activity towards HL-60 human leukaemia cells was evaluated. Among them, 3 demonstrated a clear cytotoxic activity with 72.8 μM of IC50 value.
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Masateru
authors: M
authors: Ono
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Shin
authors: S
authors: Yasuda
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Kaori
authors: K
authors: Nishi
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Kazutaka
authors: K
authors: Yamamoto
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Satoshi
authors: S
authors: Fuchizaki
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Satomi
authors: S
authors: Higuchi
authors: a Department of Bioscience, School of Agriculture , Tokai University , Kumamoto , Japan.
authors: Haruki
authors: H
authors: Komatsu
authors: b Faculty of Pharmaceutical Sciences , Fukuoka University , Fukuoka , Japan.
authors: Masafumi
authors: M
authors: Okawa
authors: b Faculty of Pharmaceutical Sciences , Fukuoka University , Fukuoka , Japan.
authors: Junei
authors: J
authors: Kinjo
authors: c Faculty of Pharmaceutical Sciences , Sojo University , Kumamoto , Japan.
authors: Hitoshi
authors: H
authors: Yoshimitsu
authors: c Faculty of Pharmaceutical Sciences , Sojo University , Kumamoto , Japan.
authors: Toshihiro
authors: T
authors: Nohara
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D065856:Rubus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D014315:Triterpenes
keywords: Rosaceae; Rubus fructicosus; blackberry; cytotoxic activity; isopropylidenedioxy; ursane-type triterpenoid