title: Gene expression profiling in adipose tissue from growing broiler chickens.
abstract: In this study, total RNA was collected from abdominal adipose tissue samples obtained from ten broiler chickens at 3, 4, 5, and 6 weeks of age and prepared for gene microarray analysis with Affymetrix GeneChip Chicken Genome Arrays (Affymetrix) and quantitative real-time PCR analysis. Studies of global gene expression in chicken adipose tissue were initiated since such studies in many animal species show that adipose tissue expresses and secretes many factors that can influence growth and physiology. Microarray results indicated 333 differentially expressed adipose tissue genes between 3 and 6 wk, 265 differentially expressed genes between 4 and 6 wk and 42 differentially expressed genes between 3 and 4 wk. Enrichment scores of Gene Ontology Biological Process categories indicated strong age upregulation of genes involved in the immune system response. In addition to microarray analysis, quantitative real-time PCR analysis was used to confirm the influence of age on the expression of adipose tissue CC chemokine ligands (CCL), toll-like receptor (TLR)-2, lipopolysaccharide-induced TNF factor (LITAF), chemokine (C-C motif) receptor 8 (CCR8), and several other genes. Between 3 and 6 wk of age CCL5, CCL1, and CCR8 expression increased (P = 0.0001) with age. Furthermore, TLR2, CCL19, and LITAF expression increased between 4 and 6 wk of age (P = 0.001). This is the first demonstration of age related changes in CCL, LITAF, and TLR2 gene expression in chicken adipose tissue. Future studies are needed to elucidate the role of these adipose tissue genes in growth and the immune system.
authors: Poultry Processing and Swine Physiology Research; Agricultural Research Service; USDA ; Athens, GA USA.
authors: Gary J
authors: GJ
authors: Hausman
authors: Poultry Processing and Swine Physiology Research; Agricultural Research Service; USDA ; Athens, GA USA.
authors: C Rick
authors: CR
authors: Barb
authors: Department of Poultry Science; University of Georgia ; Athens, GA USA.
authors: Brian D
authors: BD
authors: Fairchild
authors: Poultry Processing and Swine Physiology Research; Agricultural Research Service; USDA ; Athens, GA USA.
authors: John
authors: J
authors: Gamble
authors: Poultry Processing and Swine Physiology Research; Agricultural Research Service; USDA ; Athens, GA USA.
authors: Laura
authors: L
authors: Lee-Rutherford
keywords: adipose tissue; chicken; cytokines; gene expression