title: Genetic identification of Entamoeba polecki subtype 3 from pigs in Japan and characterisation of its pathogenic role in ulcerative colitis.
abstract: To date, three Entamoeba spp. (E. suis, zoonotic E. polecki and E. histolytica) have been identified in pigs, but their pathogenicity and molecular classification have not been fully determined. Examination and pathological analysis of pigs (n=3) with diarrhoea was conducted and revealed the presence of Entamoeba organisms. We performed a genetic analysis of the isolate using the small-subunit ribosomal RNA (SSU rRNA) gene region to identify the species. A severe ulcerative colitis was observed histopathologically with inflammatory cells, including macrophages and neutrophils, infiltrating the mucous membranes of the cecum and colon. Many Entamoeba trophozoites were found at the erosion site or at ulcerative lesions. Pathogenic viruses or bacteria were not detected. The SSU rRNA sequence of the Entamoeba isolate was found to be completely homologous to that of E. polecki subtype 3.
authors: National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan; Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
authors: Makoto
authors: M
authors: Matsubayashi
authors: Chuou Animal Health and Hygiene Service Centre of Aichi Prefecture, Okazaki, Aichi 444-0805, Japan.
authors: Naoko
authors: N
authors: Murakoshi
authors: Chuou Animal Health and Hygiene Service Centre of Aichi Prefecture, Okazaki, Aichi 444-0805, Japan.
authors: Tetsuya
authors: T
authors: Komatsu
authors: Department of Parasitology, Graduate School of Medical Science, Kanazawa University, Takara-machi, Kanazawa 920-8640, Japan.
authors: Masaharu
authors: M
authors: Tokoro
authors: National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
authors: Makoto
authors: M
authors: Haritani
authors: National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan. Electronic address: tshiba@affrc.go.jp.
authors: Tomoyuki
authors: T
authors: Shibahara
mesh_terms: D000818:Animals; D001483:Base Sequence; D003093:Colitis, Ulcerative / Q000662:veterinary*; D003106:Colon / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D004747:Entamoeba / Q000145:classification / Q000235:genetics*; D004749:Entamoebiasis / Q000662:veterinary*; D017125:Genes, Protozoan; D007413:Intestinal Mucosa / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000209:etiology* / Q000473:pathology
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: Entamoeba polecki subtype 3; Entamoeba struthionis; Pig; Ulcerative colitis