title: Ultra high performance liquid chromatography coupled to tandem mass spectrometry determination of lipid peroxidation biomarkers in newborn serum samples.
abstract: Byproducts of arachidonic (AA) and docosahexaenoic acid (DHA) oxidation are highly relevant for the study of free radical associated conditions in the perinatal period. Plasma metabolites can provide the clinician with a snapshot of the oxidant status of patients before and after specific clinical interventions (e.g.: supplementation with oxygen). We describe a new andreliable ultra-performance liquid mass spectrometry method to determine F2-isoprostanes and other byproducts (isoprostanes, isofurans, neuroprostanes, neurofurans) in newborn serum samples. Cord blood samples were obtained from severely depressed newborn infants (Apgar score 1 min < 3; arterial cord pH < 7.00), and aliquoted for serum determination and stored at -80 °C. A UHPLC-MS/MS method was employed. It has a series of technical advantages: simple sample treatment; reduced sample volume (100 μL) which is essential for preterm neonates with low circulating blood volume, high throughput of sample analysis (96 samples in less than 24 h) and high selectivity for different isoprostanes isomers. Excellent sensitivity was achieved within limits of detection between 0.06 and 4.2 nmol L(-1), which renders this method suitable to monitoranalyte concentration in newborn samples. The method's precision was satisfactory; with coefficients of variation around 5-12% (intra-day) and 7-17% (inter-day). The reliability of the described method was assessed by analysis of spiked serum samples obtaining recoveries between 70% and 120%. The proposed method has rendered suitable for serum determination for newborn babies at risk of oxygen free radical associated conditions.
authors: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors: C
authors: C
authors: Cháfer-Pericás
authors: Department of Obstetrics and Gynecology, University of Helsinki, Helsinki University Hospital, Helsinki, Finland.
authors: L
authors: L
authors: Rahkonen
authors: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors: A
authors: A
authors: Sánchez-Illana
authors: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors: J
authors: J
authors: Kuligowski
authors: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors: I
authors: I
authors: Torres-Cuevas
authors: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain.
authors: M
authors: M
authors: Cernada
authors: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain.
authors: E
authors: E
authors: Cubells
authors: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain.
authors: A
authors: A
authors: Nuñez-Ramiro
authors: Children's Hospital, University of Helsinki, Helsinki University Hospital, Helsinki, Finland.
authors: S
authors: S
authors: Andersson
authors: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain; Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain.
authors: M
authors: M
authors: Vento
authors: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain. Electronic address: justo.escobar@uv.es.
authors: J
authors: J
authors: Escobar
mesh_terms: D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn / Q000097:blood; D057230:Limit of Detection; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D008055:Lipids
keywords: Isoprostanes; Liquid chromatography; Mass spectrometry; Newborn; Oxidative stress; Serum