title: A simple extraction method for the simultaneous detection of tetramisole and diethylcarbamazine in milk, eggs, and porcine muscle using gradient liquid chromatography-tandem mass spectrometry.
abstract: Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 °C for 1h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R(2) ⩾ 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were <20%. The limit of quantification was 0.2 and 2 ng/g for tetramisole and diethylcarbamazine, respectively. An analytical survey of samples purchased from large markets showed that none of the samples contained any of the target analytes. To the best of our knowledge, this is the first report on the quantitative determination of tetramisole and diethylcarbamazine in animal food products.
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Dan
authors: D
authors: Zhang
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Jin-A
authors: JA
authors: Park
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Dong-Soon
authors: DS
authors: Kim
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Seong-Kwan
authors: SK
authors: Kim
authors: Department of Molecular and Cell Biology, University of California Berkeley, 142 Life Sciences Addition #3200, Berkeley, CA 94720, United States.
authors: Soo-Jean
authors: SJ
authors: Shin
authors: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
authors: Jae-Han
authors: JH
authors: Shim
authors: Department of Chemistry and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Sung Chul
authors: SC
authors: Shin
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Jin-Suk
authors: JS
authors: Kim
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. Electronic address: abdelaty44@hotmail.com.
authors: A M
authors: AM
authors: Abd El-Aty
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: hshin@konkuk.ac.kr.
authors: Ho-Chul
authors: HC
authors: Shin
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004049:Diethylcarbamazine / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D013773:Tetramisole / Q000032:analysis*
chemical_list: D013773:Tetramisole; D004049:Diethylcarbamazine
keywords: Anthelmintics; Diethylcarbamazine; Eggs; LC/MS/MS; Milk; Porcine muscle; Tetramisole