title: Genomic detection and characterization of a Korean isolate of Little cherry virus 1 sampled from a peach tree.
abstract: A peach tree (Prunus persica) showing yellowing and mild mottle symptoms was analyzed using high-throughput RNA sequencing to determine the causal agent. A total of nine contigs similar to Little cherry virus 1 (LChV-1) were produced, and all the contigs showed nucleotide sequence identity (lower than 83 %) and query coverage (higher than 73 %) with LChV-1. The symptomatic peach sample was confirmed to be infected with LChV-1-like virus as a result of reverse transcription-polymerase chain reaction using primers designed based on sequences of the contigs. Occurrence of diseases caused by LChV-1 in Prunus species has been reported. Complete 16,931-nt genome of the peach virus composed of eight open reading frames was determined, and conserved domains including viral methyltransferase, viral helicase 1, RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homologue (HSP70h), HSP90h and closterovirus coat protein (CP) were identified. Phylogenetic trees based on amino acid sequence alignments between the peach virus and members in the family Closteroviridae showed that the virus was most similar to LChV-1. Pairwise comparisons based on amino acid sequence alignments of three genes (RdRp, HSP70h and CP) between the peach virus and LChV-1 isolates showed the highest amino acid sequence identities, with 84.32 % for RdRp, 85.48 % for HSP70h and 80.45 % for CP. These results indicate that this is the first report for the presence of LChV-1 in South Korea and may be one of the first reports of natural infection of peach by LChV-1. Although it is not clear if LChV-1 YD isolate was responsible for specific symptoms observed, detection and characterization of the peach tree-infecting LChV-1 in South Korea would be useful in terms of the epidemiology of LChV-1.
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors: Seungmo
authors: S
authors: Lim
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors: Davaajargal
authors: D
authors: Igori
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors: Ran Hee
authors: RH
authors: Yoo
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors: Fumei
authors: F
authors: Zhao
authors: Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 441-440, Republic of Korea.
authors: In-Sook
authors: IS
authors: Cho
authors: Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 441-440, Republic of Korea.
authors: Gug-Seoun
authors: GS
authors: Choi
authors: Department of Applied Biology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Hyoun-Sub
authors: HS
authors: Lim
authors: School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea. suheon@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea. suheon@knu.ac.kr.
authors: Su-Heon
authors: SH
authors: Lee
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea. jsmoon@kribb.re.kr.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea. jsmoon@kribb.re.kr.
authors: Jae Sun
authors: JS
authors: Moon
mesh_terms: D029381:Closteroviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D007723:Korea; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D000068239:Prunus persica / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral
keywords: Closteroviridae; LChV-1; Peach; Velarivirus