title: [Experimental Study on Apoptosis of Kasumi-1 Cells Induced by Sertraline and Its Molecular Mechanism].
abstract: OBJECTIVE
  To investigate the biological effects of sertraline, one of psychotropic drugs, on actue myeloid leukemia cell line Kasumi-1.
  
  
  METHODS
  Cells were treated by different concentrations of sertraline for different times. The effects of sertraline were evaluated by cell growth, cell morphology, cell cycle distribution and markers of cell apoptosis, respectively. Western blot was used to detect the expression change of related proteins.
  
  
  RESULTS
  Sertraline could inhibit cell proliferation and induce apoptosis. After treatment with 15 µmol/L and 20 µmol/L sertraline for 24 h, the inhibitory rate of Kasumi-1 cell proliferation was (19.00 ± 7.37)% and (47.90 ± 11.19)%, respectively. Meanwhile, compared with the control group, the percentage of Annexin V positive cells in Kasumi-1 cells treated with sertraline for 24 h raised obviously from (9.71 ± 2.12)% to (20.54 ± 2.52)% and (45.37 ± 7.88)% (P < 0.01), respectively. The cells were arrested in G0/G1 and G2/M phase. In addition, it was found that sertraline could also down-regulate the level of translationally controlled tumor protein (TCTP) in Kasumi-1 cells.
  
  
  CONCLUSIONS
  Sertraline can significantly induce the apoptosis of Kasumi-1 cells, that probably is associated with the down-regulation of TCTP protein expression.
authors: State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Wei-Wei
authors: WW
authors: Yan
authors: State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Di
authors: D
authors: Xia
authors: State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Ying-Ting
authors: YT
authors: Zhang
authors: State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors: Xiao-Rong
authors: XR
authors: Pan
authors: State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. E-mail: jh_tong@126.com.
authors: Jian-Hua
authors: JH
authors: Tong
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D020280:Sertraline; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1; D020280:Sertraline