title: Growth condition-dependent cell surface proteome analysis of Enterococcus faecium.
abstract: The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was grown until mid-log phase in brain heart infusion medium (BHI) with, or without 0.02% bile salts, Tryptic Soy Broth with 1% glucose (TSBg) and urine, and its cell surface was "shaved" using immobilized trypsin. Peptides were identified using MS/MS. Mapping against the translated E1162 whole genome sequence identified 67 proteins that were differentially detected in different conditions. In urine, 14 proteins were significantly more and nine proteins less abundant relative to the other conditions. Growth in BHI-bile and TSBg, revealed four and six proteins, respectively, which were uniquely present in these conditions while two proteins were uniquely present in both conditions. Thus, proteolytic shaving of E. faecium cells identified differentially surface exposed proteins in different growth conditions. These proteins are of special interest as they provide more insight in the adaptive mechanisms and may serve as targets for the development of novel therapeutics against this multi-resistant emerging pathogen. All MS data have been deposited in the ProteomeXchange with identifier PXD002497 (http://proteomecentral.proteomexchange.org/dataset/PXD002497).
authors: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Jan C
authors: JC
authors: Sinnige
authors: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Mark
authors: M
authors: de Been
authors: Fungal Biodiversity Centre, Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands.|Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
authors: Miaomiao
authors: M
authors: Zhou
authors: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Marc J M
authors: MJ
authors: Bonten
authors: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Rob J L
authors: RJ
authors: Willems
authors: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Janetta
authors: J
authors: Top
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry; D016000:Cluster Analysis; D003198:Computer Simulation; D003428:Cross Infection / Q000382:microbiology; D003470:Culture Media; D016984:Enterococcus faecium / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D008565:Membrane Proteins; D020543:Proteome
keywords: Enterococcus faecium; Growth; MS; Microbiology; Shaving; Surfacome