title: Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy.
abstract: Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identification of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity. Our focus is on theranostics of visceral leishmaniasis with the aim to develop biomarker candidates exhibiting both diagnostic and therapeutic potential to fight this important, yet neglected, tropical disease.
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BR.
authors: Eduardo Antonio Ferraz
authors: EA
authors: Coelho
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BR.
authors: Miguel Angel
authors: MA
authors: Chávez-Fumagalli
authors: Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BR.
authors: Lourena Emanuele
authors: LE
authors: Costa
authors: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BR.
authors: Carlos Alberto Pereira
authors: CA
authors: Tavares
authors: Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autonoma de Madrid, Universidad Autónoma de Madrid, Madrid, ES.
authors: Manuel
authors: M
authors: Soto
authors: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, BR.
authors: Luiz Ricardo
authors: LR
authors: Goulart
mesh_terms: D000818:Animals; D015415:Biomarkers; D001709:Biotechnology; D061505:Cell Surface Display Techniques / Q000379:methods*; D055808:Drug Discovery / Q000379:methods; D005821:Genetic Techniques; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007896:Leishmaniasis / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination
chemical_list: D015415:Biomarkers