title: Transference of CALIPER pediatric reference intervals to biochemical assays on the Roche cobas 6000 and the Roche Modular P.
abstract: OBJECTIVE
  The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has recently established pediatric age- and sex-specific reference intervals for over 85 biochemical markers on the Abbott Architect system. Previously, CALIPER reference intervals for several biochemical markers were successfully transferred from Abbott assays to Roche, Beckman, Ortho, and Siemens assays. This study further broadens the CALIPER database by performing transference and verification for 52 biochemical assays on the Roche cobas 6000 and the Roche Modular P.
  
  
  METHODS
  Using CLSI C28-A3 and EP9-A2 guidelines, transference of the CALIPER reference intervals was attempted for 16 assays on the Roche cobas 6000 and 36 on the Modular P. Calculated reference intervals were further verified using 100 healthy CALIPER samples.
  
  
  RESULTS
  Most assays showed strong correlation between assay systems and were transferable from Abbott to the Roche cobas 6000 (81%) and the Modular P (86%). Bicarbonate and magnesium were not transferable on either system and calcium and prealbumin were not transferable to the Modular P. Of the transferable analytes, 62% and 61% were verified on the cobas 6000 and the Modular P, respectively.
  
  
  CONCLUSIONS
  This study extends the utility of the CALIPER database to two additional analytical systems, which facilitates the broad application of CALIPER reference intervals at pediatric centers utilizing Roche biochemical assays. Transference studies across different analytical platforms can later be collectively analyzed in an attempt to develop common reference intervals across all clinical chemistry instruments to harmonize laboratory test interpretation in diagnosis and monitoring of pediatric disease.
authors: CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Canada; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada.
authors: Victoria
authors: V
authors: Higgins
authors: CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Canada.
authors: Man Khun
authors: MK
authors: Chan
authors: CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Canada.
authors: Michelle
authors: M
authors: Nieuwesteeg
authors: Laboratory Medicine and Pathobiology, Mount Sinai Hospital, Toronto, ON, Canada.
authors: Barry R
authors: BR
authors: Hoffman
authors: Laboratory Medicine and Pathobiology, Mount Sinai Hospital, Toronto, ON, Canada.
authors: Irvin L
authors: IL
authors: Bromberg
authors: Department of Pathology, Toronto East General Hospital, Toronto, ON, Canada.
authors: Doug
authors: D
authors: Gornall
authors: Eastern Health, St. John's, NL, Canada.
authors: Edward
authors: E
authors: Randell
authors: CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Canada; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada. Electronic address: khosrow.adeli@sickkids.ca.
authors: Khosrow
authors: K
authors: Adeli
mesh_terms: D000293:Adolescent; D000328:Adult; D002624:Chemistry, Clinical / Q000295:instrumentation*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D010372:Pediatrics; D012016:Reference Values; D055815:Young Adult
keywords: Abbott Architect; CALIPER; Pediatrics; Reference intervals; Roche Modular P; Roche cobas 6000