title: An on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-total antioxidant capacity detection system applying two antioxidant methods for activity evaluation of the edible flowers from Prunus mume.
abstract: An on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-total antioxidant capacity detection (HPLC-DAD-ESI-IT-TOF-MS-TACD) system was created for identification and evaluation of antioxidants in Prunus (P.) mume flowers. Applying this system, the HPLC fingerprint, ultraviolet (UV) spectra, mass fragmentations, active profiles against 1,1-diphenylpicryl-2-hydrazyl radical (DPPH•) scavenging activity and ferric reducing antioxidant power (FRAP) of each complex sample were obtained simultaneously after one injection. Synchronous structure identification and activities screening of complex samples were thus accomplished. In this study, 78 compounds were identified from P. mume flowers by their chromatographic behaviors, UV spectra and MS data with the assistance of standard compounds and literature reports. The DPPH and FRAP activity of 24 samples (23 different P. mume varieties and 1 related herbal medicine) were then quantified by their detailed activity profiles from the on-line system, and by the total activity of each sample extract from off-line 96-well plate method. As a result, 21 and 32 compounds in the on-line system showed anti-oxidative effects against DPPH and FRAP, respectively. The established on-line system is efficient, sensitive and reliable to tell the DPPH and FRAP antioxidant activities of individual compound in complex samples, and therefore would be a useful and promising technique for antioxidant screening from different food and medicinal matrices.
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: zxiaoxia0512@163.com.
authors: Xiaoxia
authors: X
authors: Zhang
authors: Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors: Zongtao
authors: Z
authors: Lin
authors: College of horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jinggui
authors: J
authors: Fang
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Meixian
authors: M
authors: Liu
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Yanyan
authors: Y
authors: Niu
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: chenshizhong66@163.com.
authors: Shizhong
authors: S
authors: Chen
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: hw9505@bjmu.edu.cn.
authors: Hong
authors: H
authors: Wang
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D035264:Flowers / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; DPPH; FRAP; HPLC-DAD-ESI-IT-TOF-MS-TACD; On-line method; Prunus mume flower