title: Influence of the hTERT rs2736100 polymorphism on telomere length in gastric cancer.
abstract: OBJECTIVE
  To investigate the functional consequences of rs2736100 polymorphism in telomere length and examine its link to gastric cancer risk.
  
  
  METHODS
  Telomere length and human telomerase reverse transcriptase (hTERT) mRNA expression were measured in 35 gastric cancer tissues and 5 cell lines and correlated to rs2736100 polymorphism. The relationship between rs2736100 polymorphism and the risk of gastric cancer were examined in 243 gastric cancer patients and 246 healthy individuals.
  
  
  RESULTS
  The rs2736100 A allele carrier is closely associated with reduced hTERT mRNA expression and shortened telomere length in gastric cancer tissue and cell lines. When gastric cancers were stratified by histological subtype, telomere length and hTERT mRNA levels were significantly increased in those with the C/C genotype in intestinal-type gastric cancer, but not in diffuse-type gastric cancer. Interestingly, there was no significant difference in the genotype and allele frequencies of the rs2736100 polymorphism between the patients with gastric cancer and healthy controls.
  
  
  CONCLUSIONS
  The rs2736100 polymorphism of the hTERT gene is involved in the regulation of hTERT expression and telomere length, but not in the risk of gastric cancer.
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Byung Joon
authors: BJ
authors: Choi
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Jung Hwan
authors: JH
authors: Yoon
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Olga
authors: O
authors: Kim
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Won Suk
authors: WS
authors: Choi
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Suk Woo
authors: SW
authors: Nam
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Jung Young
authors: JY
authors: Lee
authors: Byung Joon Choi, Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
authors: Won Sang
authors: WS
authors: Park
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D059505:Telomere Homeostasis; D055815:Young Adult
chemical_list: C509186:TERT protein, human; D019098:Telomerase
keywords: Gastric cancer; Human telomerase reverse transcriptase; Polymorphism; Risk; Telomere