title: Blood Volume Required for Detection of Low Levels and Ultralow Levels of Organisms Responsible for Neonatal Bacteremia by Use of Bactec Peds Plus/F, Plus Aerobic/F Medium, and the BD Bactec FX System: an In Vitro Study.
abstract: We used an in vitro technique to investigate blood volumes required to detect bacteremia and fungemia with low concentrations of an organism. At 1 to 10 CFU/ml, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, Listeria monocytogenes, Candida albicans, and Candida parapsilosis isolates were detected in volumes as low as 0.5 ml. Detection of Streptococcus agalactiae and detection of bacteremia at <1 CFU/ml were unreliable.
authors: Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
authors: Diana P
authors: DP
authors: Lancaster
authors: Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors: David F
authors: DF
authors: Friedman
authors: Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
authors: Kathleen
authors: K
authors: Chiotos
authors: Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA otak@chop.edu.
authors: Kaede V
authors: KV
authors: Sullivan
mesh_terms: D058491:Bacterial Load; D002177:Candidiasis / Q000175:diagnosis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004927:Escherichia coli Infections / Q000175:diagnosis*; D006801:Humans; D007231:Infant, Newborn; D008828:Microbiological Techniques / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013290:Streptococcal Infections / Q000175:diagnosis*