title: Isolation and characterization of benzo[a]pyrene-degrading bacteria from the Tokyo Bay area and Tama River in Japan.
abstract: Benzo[a]pyrene (BaP) is one of the polycyclic aromatic hydrocarbons, and has serious detrimental effects on human health and aquatic environments. In this study, we isolated nine bacterial strains capable of degrading BaP from the Tokyo Bay area and Tama River in Japan. The isolated bacteria belonged to the phyla Actinobacteria, Firmicutes, Proteobacteria and Bacteroidetes, indicating that the BaP-degrading bacteria were widely present in the hydrosphere. ITB11, which shared 100% 16S rRNA identity with Mesoflavibacter zeaxanthinifaciens in the phylum Bacteroidetes, showed the highest degradation of BaP (approximately 86%) among the nine isolated strains after 42 days. Moreover, it was found that three of the nine isolated strains collectively removed 50-55% of BaP during the first 7 days. Growth measurement of M. zeaxanthinifaciens revealed that the strain utilized BaP as a sole carbon and energy source and salicylate acted only as an inducer of BaP degradation.
authors: Department of Ocean Sciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors: Masahiko
authors: M
authors: Okai
authors: Department of Ocean Sciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors: Ikumi
authors: I
authors: Kihara
authors: Department of Ocean Sciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors: Yuto
authors: Y
authors: Yokoyama
authors: Department of Ocean Sciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors: Masami
authors: M
authors: Ishida
authors: Department of Ocean Sciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan urano@kaiyodai.ac.jp.
authors: Naoto
authors: N
authors: Urano
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D061006:Bays / Q000382:microbiology*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001673:Biodegradation, Environmental; D000068536:Bacillota / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007564:Japan; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D012988:Soil Microbiology; D014041:Tokyo; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D001564:Benzo(a)pyrene
keywords: Bacteroidetes; benzo[a]pyrene; bioremediation; degradation; polycyclic aromatic hydrocarbon; pyrene