title: Up-Regulation of MiR-452 Inhibits Metastasis of Non-Small Cell Lung Cancer by Regulating BMI1.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) have been regarded as a new class of regulators in cellular processes in non-small cell lung cancer (NSCLC). However, the relationship between miR-452 and the development of NSCLC remains unclear.
  
  
  METHODS
  qRT-PCR was used to detect the expression of miR-452 and its target gene in NSCLC samples (n=60). The transwell assay was used to test the cell invasion capability. The regulation mechanism was confirmed by luciferase reporter assay and western blot assay.
  
  
  RESULTS
  In the current study, a relatively lower miR-452 and higher BMI1 expression levels were confirmed to be associated with advanced tumor stage and more extent of lymph nodes metastasis. In vitro, down-regulated miR-452 could enhance cell invasion capability. Furthermore, miR-452 modulated BMI1 expression by binding to its 3'-UTR. The enhancement of cell invasion capability induced by down-regulated miR-452 was eliminated by repression of BMI1.
  
  
  CONCLUSIONS
  Our results suggest that miR-452 plays a vital role in development of NSCLC, and this miR-452-BMI1 pathway might generate a novel insight into the treatment of NSCLC.
authors: Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors: Zhicheng
authors: Z
authors: He
authors: Yang
authors: Y
authors: Xia
authors: Chunfeng
authors: C
authors: Pan
authors: Teng
authors: T
authors: Ma
authors: Bin
authors: B
authors: Liu
authors: Juejin
authors: J
authors: Wang
authors: Liang
authors: L
authors: Chen
authors: Yijiang
authors: Y
authors: Chen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D063150:Polycomb Repressive Complex 1 / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C069077:BMI1 protein, human; C574051:MIRN452 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1