title: A highly sensitive and selective fluorescent probe for trivalent aluminum ion based on rhodamine derivative in living cells.
abstract: A rhodamine spirolactam derivative (1) is developed as a colormetric and fluorescent probe for trivalent aluminum ions (Al(3+)). It exhibits a highly sensitive "turn-on" fluorescent response toward Al(3+) with a 70-fold fluorescence intensity enhancement under 2 equiv. of Al(3+) added. The probe can be applied to the quantification of Al(3+) with a linear range covering from 5.0 × 10(-7) to 2.0 × 10(-5) M and a detection limit of 4.0 × 10(-8) M. Most importantly, the fluorescence changes of the probe are remarkably specific for Al(3+) in the presence of other metal ions, which meet the selective requirements for practical application. Moreover, the experiment results show that the response behavior of 1 towards Al(3+) is pH independent in neutral condition (pH 6.0-8.0) and the response of the probe is fast (response time less than 3 min). In addition, the proposed probe has been used to detect Al(3+) in water samples and image Al(3+) in living cells with satisfying results.
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China.
authors: Jia-Liang
authors: JL
authors: Tang
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China. Electronic address: lichunyan79@sina.com.
authors: Chun-Yan
authors: CY
authors: Li
authors: College of Chemical Engineering, Xiangtan University, Xiangtan, 411105, PR China.
authors: Yong-Fei
authors: YF
authors: Li
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China.
authors: Xi
authors: X
authors: Lu
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China. Electronic address: hongruiqi@163.com.
authors: Hong-Rui
authors: HR
authors: Qi
mesh_terms: D000535:Aluminum / Q000032:analysis*; D002412:Cations / Q000032:analysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D005618:Fresh Water / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D012235:Rhodamines; D000535:Aluminum
keywords: Aluminum ion; Cell imaging; Fluorescence; Off-on; Probe; Rhodamine