title: Nano-mole scale sequential signal assignment by (1)H-detected protein solid-state NMR.
abstract: We present a 3D (1)H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D (13)C-detected SSNMR approach.
authors: Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors: Songlin
authors: S
authors: Wang
authors: Sudhakar
authors: S
authors: Parthasarathy
authors: Yiling
authors: Y
authors: Xiao
authors: Yusuke
authors: Y
authors: Nishiyama
authors: Fei
authors: F
authors: Long
authors: Isamu
authors: I
authors: Matsuda
authors: Yuki
authors: Y
authors: Endo
authors: Takahiro
authors: T
authors: Nemoto
authors: Kazuo
authors: K
authors: Yamauchi
authors: Tetsuo
authors: T
authors: Asakura
authors: Mitsuhiro
authors: M
authors: Takeda
authors: Tsutomu
authors: T
authors: Terauchi
authors: Masatsune
authors: M
authors: Kainosho
authors: Yoshitaka
authors: Y
authors: Ishii
mesh_terms: D011506:Proteins / Q000032:analysis*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D011506:Proteins