title: Pinocembrin reduces cardiac arrhythmia and infarct size in rats subjected to acute myocardial ischemia/reperfusion.
abstract: Oxidative stress plays an important role in the pathogenesis of ischemia/reperfusion (I/R) injury induced by cardiac dysfunction. Pinocembrin (5,7-dihydroxyflavanone) is a flavonoid found in propolis and in rhizomes of fingerroot (Boesenbergia pandurata) that is reported to have pharmacological properties including antimicrobial, antioxidant, and anti-inflammatory activities. The cardioprotective effects of pinocembrin in an I/R model were investigated in this study. Male Wistar rats (n = 20) were randomly divided into 2 groups to receive either pinocembrin (30 mg/kg body weight) or the vehicle intravenously. Thirty minutes later, the left anterior descending coronary artery of each rat was ligated for 30 min, and then reperfusion was allowed for 120 min. Cardiac function improved in the pinocembrin-treated group: the time to first ventricular fibrillation (VF) was significantly longer in the treated group (550 ± 54 s) than in the vehicle-only control group (330 ± 27 s) (p < 0.05). VF incidence and arrhythmia score were lower and infarcts were 49% smaller in the pinocembrin-treated group than in the control group (p < 0.05). In the pinocembrin-treated group, malondialdehyde levels and Bax/Bcl-2 ratios decreased, and the ratio of phosphorylated connexin 43 (phospho-Cx43) to total Cx43 increased in infarcted tissues compared with the non-infarcted area (p < 0.05). Pinocembrin exhibited cardioprotective effects during I/R, evidenced by improved cardiac function, fewer arrhythmias, and smaller infarcts in treated hearts than in controls. These benefits may be due to pinocembrin's antiapoptotic and anti-oxidative stress effects and its ability to increase the phosphorylation of Cx43 in ischemic myocardium.
authors: a Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|c Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
authors: Anusorn
authors: A
authors: Lungkaphin
authors: a Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|c Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
authors: Anchalee
authors: A
authors: Pongchaidecha
authors: a Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|c Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.|d School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand.
authors: Siripong
authors: S
authors: Palee
authors: e Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
authors: Phatchawan
authors: P
authors: Arjinajarn
authors: f Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Lampang Rajabhat University, Lampang, Thailand.
authors: Wilart
authors: W
authors: Pompimon
authors: a Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.|c Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
authors: Nipon
authors: N
authors: Chattipakorn
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001145:Arrhythmias, Cardiac / Q000517:prevention & control*; D002316:Cardiotonic Agents / Q000494:pharmacology; D044950:Flavanones / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D044950:Flavanones; C016063:pinocembrin
keywords: acute myocardial ischemia/reperfusion; cardiac function; fonction cardiaque; ischémie/reperfusion aigüe du myocarde; pinocembrin; pinocembrine