title: Oxidative stress associated with pathological lesions in the liver of rats experimentally infected by Fasciola hepatica.
abstract: The aim of this study was to analyze the antioxidant status and oxidative profile in serum and liver of rats experimentally infected with Fasciola hepatica and its relationship with pathological findings. Twenty-four rats were divided into two groups: group A consisted of 12 healthy rats and group B of 12 rats infected orally with 20 metacercaria of F. hepatica. At days 20 and 150 post-infection (PI), blood and liver samples of six animals from each group were collected. The protein oxidation (AOPP technique: advanced oxidation protein products) and antioxidants (FRAP technique: ferric reducing antioxidant power) levels were measured in serum and liver. Furthermore, nitrite/nitrate (NOx) levels and lipid peroxidation (TBARS technique: thiobarbituric acid reactive substances) were measured in liver. AOPP and FRAP levels were increased (P < 0.05) in serum and liver of infected animals in acute and chronic infection when compared with healthy animals. The same occurred with TBARS and NOx levels in the liver (P < 0.05). Histopathology revealed periportal fibrous hepatitis, composed of an abundant inflammatory infiltrate in portal spaces on infected animals, as well as bile duct hyperplasia. The results found seem to be related to the host free radical production demonstrated in serum samples and liver due to the parasite infection.
authors: Department of Biochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors: Nathieli B
authors: NB
authors: Bottari
authors: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors: Ricardo E
authors: RE
authors: Mendes
authors: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors: Neuber J
authors: NJ
authors: Lucca
authors: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors: Claiton I
authors: CI
authors: Schwertz
authors: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors: Luan C
authors: LC
authors: Henker
authors: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors: Débora C
authors: DC
authors: Olsson
authors: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors: Manoela M
authors: MM
authors: Piva
authors: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Center of Health Sciences, UFSM, Santa Maria, RS, Brazil.
authors: Manuela
authors: M
authors: Sangoi
authors: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Center of Health Sciences, UFSM, Santa Maria, RS, Brazil.
authors: Luízi P
authors: LP
authors: Campos
authors: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Center of Health Sciences, UFSM, Santa Maria, RS, Brazil.
authors: Rafael N
authors: RN
authors: Moresco
authors: Laboratory of General Biochemistry and Microorganisms, Center of Biological and Health Sciences, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors: Jeandre A
authors: JA
authors: Jaques
authors: Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
authors: Aleksandro S
authors: AS
authors: Da silva
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D005211:Fascioliasis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology*; D008103:Liver Cirrhosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D008297:Male; D009566:Nitrates / Q000032:analysis; D009573:Nitrites / Q000032:analysis; D018384:Oxidative Stress; D051381:Rats; D012756:Sheep; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D062107:Advanced Oxidation Protein Products; D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D017392:Thiobarbituric Acid Reactive Substances
keywords: Fasciolosis; Hepatic function; Lipid peroxidation; Protein oxidation