title: Circulating brain derived neurotrophic factor (BDNF) and frequency of BDNF positive T cells in peripheral blood in human ischemic stroke: Effect on outcome.
abstract: The aim of this study was to measure the levels of circulating BDNF and the frequency of BDNF-producing T cells after acute ischaemic stroke. Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against markers of T cells, T regulatory cells (Tregs), and intracellular BDNF. There was a slight increase in serum BDNF levels after stroke. There was no overall difference between stroke patients and controls in the frequency of CD4(+) and CD8(+) BDNF(+) cells, although a subgroup of stroke patients showed high frequencies of these cells. However, there was an increase in the percentage of BDNF(+) Treg cells in the CD4(+) population in stroke patients compared to controls. Patients with high percentages of CD4(+) BDNF(+) Treg cells had a better outcome at 6months than those with lower levels. These groups did not differ in age, gender or initial stroke severity. Enhancement of BDNF production after stroke could be a useful means of improving neuroprotection and recovery after stroke.
authors: University of Queensland, Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia.
authors: Adeline
authors: A
authors: Chan
authors: University of Queensland, Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia.
authors: Jun
authors: J
authors: Yan
authors: University of Queensland, Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia.
authors: Peter
authors: P
authors: Csurhes
authors: University of Queensland, Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia.
authors: Judith
authors: J
authors: Greer
authors: University of Queensland, Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia. Electronic address: Pamela.mccombe@uq.edu.au.
authors: Pamela
authors: P
authors: McCombe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D020521:Stroke / Q000097:blood* / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D019208:Brain-Derived Neurotrophic Factor
keywords: Acute ischemic stroke; Brain derived neurotrophic factor; Flow cytometry; FoxP3; Neuroprotection; Protective immunity; Regulatory T cells; Stroke outcome