title: Complementary LC-MS/MS Proteomic Analysis of Uremic Plasma Proteins.
abstract: OBJECTIVE
  To complement an earlier analysis of protein alterations in plasma from uremic versus healthy subjects by addition of further LC-MS/MS analysis to the previously used MALDI-TOF mass analyses.
  
  
  METHODS
  Sequence identifications of tryptic peptides from SDS gel electrophoretic fractions of immunodepleted and HPLC-fractionated plasma was performed from seven chronic kidney disease stage 5 patients (age 55 ± 14 years, glomerular filtration rate 6.9 ±2.9 mL/minute/1.73 m2) and from seven matched controls.
  
  
  RESULTS
  About twice as many proteins were increased in uremic plasma as the previously identified. The identifications included proteins that consistently complement the two identification patterns regarding separate subunits from the same protein complex.
  
  
  CONCLUSIONS
  Mass spectrometric analysis is applicable to complex plasma proteomes in clinical settings. The LC-MS/MS technique, based on individual peptide sequence analyses, gives increased identifications and also demonstrates feasibility of this technique in clinical practice.
authors: Department of Medical Biochemistry and Biophysics / Clinical Science, Intervention and Technology2, Karolinska Institutet, Stockholm, Sweden. / National Center for Proteomics, University of Karachi, Karachi.
authors: Uzma
authors: U
authors: Naseeb
authors: Department of Medical Biochemistry and Biophysics / Clinical Science, Intervention and Technology2, Karolinska Institutet, Stockholm, Sweden.
authors: Jonas
authors: J
authors: Axelsson
authors: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: Theres
authors: T
authors: Jägerbrink
authors: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: Jawed
authors: J
authors: Shafqat
authors: National Center for Proteomics, University of Karachi, Karachi.
authors: Shamshad
authors: S
authors: Zarina
authors: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: Hans
authors: H
authors: Jörnvall
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D019317:Evidence-Based Medicine; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins