title: A Simple Method for Human Whole Blood Microcultures and Its Application in Radiation Biodosimetry.
abstract: The scoring of the cytokinesis-block micronucleus and dicentric chromosomes in human peripheral blood lymphocytes is used as a dosimeter of radiation exposure. A detailed methodology is presented for human whole blood microculture for cytogenetic analysis. The technique described yields more than sufficient numbers of mitotic lymphocytes for analyzing micronuclei and chromosome aberrations following exposure to radiation.
authors: *Shenzhen Major Occupation Disease Diagnosis and Treatment Technology Key Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen, China.
authors: Guo
authors: G
authors: Yan
authors: Zhang
authors: Z
authors: Wen
authors: Gao
authors: G
authors: Chaoxian
authors: Hui
authors: H
authors: Changye
authors: Yang
authors: Y
authors: Xueqin
authors: Yang
authors: Y
authors: Xinyue
authors: Li
authors: L
authors: Zhimin
mesh_terms: D065638:Absorption, Radiation; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D011829:Radiation Dosage; D011834:Radiation Monitoring / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity