title: Hybrid random walk-linear discriminant analysis method for unwrapping quantitative phase microscopy images of biological samples.
abstract: Standard algorithms for phase unwrapping often fail for interferometric quantitative phase imaging (QPI) of biological samples due to the variable morphology of these samples and the requirement to image at low light intensities to avoid phototoxicity. We describe a new algorithm combining random walk-based image segmentation with linear discriminant analysis (LDA)-based feature detection, using assumptions about the morphology of biological samples to account for phase ambiguities when standard methods have failed. We present three versions of our method: first, a method for LDA image segmentation based on a manually compiled training dataset; second, a method using a random walker (RW) algorithm informed by the assumed properties of a biological phase image; and third, an algorithm which combines LDA-based edge detection with an efficient RW algorithm. We show that the combination of LDA plus the RW algorithm gives the best overall performance with little speed penalty compared to LDA alone, and that this algorithm can be further optimized using a genetic algorithm to yield superior performance for phase unwrapping of QPI data from biological samples.
authors: University of California, Los Angeles, Department of Bioengineering, TLSB 3126, California 90095, United States.
authors: Diane N H
authors: DN
authors: Kim
authors: University of California, Los Angeles, Department of Bioengineering, TLSB 3126, California 90095, United StatesbUniversity of California, Los Angeles, Jonsson Comprehensive Cancer Center, 8-950 Factor Building, California 90095, United StatescUniversity o.
authors: Michael A
authors: MA
authors: Teitell
authors: Virginia Commonwealth University, Department of Physics, 701 West Grace Street, Richmond, Virginia 23284, United StateshVirginia Commonwealth University, Massey Cancer Center, 401 College Street, Richmond, Virginia 23284, United States.
authors: Jason
authors: J
authors: Reed
authors: University of California, Los Angeles, Department of Bioengineering, TLSB 3126, California 90095, United StatesbUniversity of California, Los Angeles, Jonsson Comprehensive Cancer Center, 8-950 Factor Building, California 90095, United StatescUniversity o.
authors: Thomas A
authors: TA
authors: Zangle
mesh_terms: D000465:Algorithms; D000818:Animals; D045744:Cell Line, Tumor; D016002:Discriminant Analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D012372:ROC Curve