title: Reply to Shoji and Katsuma.
abstract: Our paper reports a novel strategy for the artificial introduction of DNA methylation in mouse gonocytes. The manuscript presents data showing that the concomitant expression of sense and anti-sense of EGFP transgenes in embryonic male germ cells induces gene silencing via the piRNA pathway and that the expression of an antisense Dnmt3L transgene induces silencing of the endogenous Dnmt3L gene.
authors: Department of Pathology, Medical School, Osaka University, Yamada-oka 2-2 Suita, Osaka 565-0871, Japan.
authors: Satomi
authors: S
authors: Kuramochi-Miyagawa
authors: Department of Pathology, Medical School, Osaka University, Yamada-oka 2-2 Suita, Osaka 565-0871, Japan; Department of Graduate School of Frontier Biosciences, Osaka University, Yamada-oka 2-2 Suita, Osaka 565-0871, Japan. Electronic address: tnakano@patho.med.osaka-u.ac.jp.
authors: Toru
authors: T
authors: Nakano
mesh_terms: D000818:Animals; D019175:DNA Methylation; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D034741:RNA, Small Interfering
chemical_list: D034741:RNA, Small Interfering