title: Synergistic interactions of apigenin, naringin, quercetin and emodin on inhibition of 3T3-L1 preadipocyte differentiation and pancreas lipase activity.
abstract: The interactions of four natural compounds including apigenin, naringin, emodin and quercetin were investigated on inhibiting 3T3-L1 preadipocyte differentiation and pancreas lipase activity. Oil Red O staining was conducted to visualise and quantify lipid accumulation. The difference between experimental and calculated results was utilised for determining the interaction types. Interestingly, emodin synergistically interacted with the other three compounds, and the combination of emodin and apigenin exhibited the strongest synergistic effect in both differentiation and pancreas lipase assays. Results implied that the combination of apigenin and emodin may be regarded as a promising complementary therapy for management of overweight or obesity.
authors: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors: XiaoXuan
authors: X
authors: Guo
authors: China National Research Institute of Food and Fermentation Industries, Beijing 100027, People's Republic of China.
authors: Jia
authors: J
authors: Liu
authors: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan Province 650500, People's Republic of China.
authors: ShengBao
authors: S
authors: Cai
authors: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors: Ou
authors: O
authors: Wang
authors: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China. Electronic address: baoping_ji@sina.com.
authors: BaoPing
authors: B
authors: Ji
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D047310:Apigenin / Q000494:pharmacology*; D004357:Drug Synergism; D004642:Emodin / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology*; D008049:Lipase / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D044950:Flavanones; D047310:Apigenin; D011794:Quercetin; D008049:Lipase; D004642:Emodin; C005274:naringin
keywords: Antiobesity; Natural component; Pancreas lipase; Preadipocyte differentiation; Synergistic interaction