title: Facile fabrication of an aptasensor for thrombin based on graphitic carbon nitride/TiO2 with high visible-light photoelectrochemical activity.
abstract: A novel aptasensor for thrombin with high visible-light activity was facilely fabricated based on graphitic carbon nitride/TiO2 (g-C3N4/TiO2) photoelectrochemical (PEC) composite. Crystallization of TiO2 nanoparticles (NPs) and their strong interaction with g-C3N4 sheet were confirmed by high-resolution transmission electron microscope (HR-TEM), both of which contributed to the high photocurrent intensity under visible-light irradiation. Carboxyl functionalized thrombin aptamers were first successfully bound to the g-C3N4/TiO2 modified electrode as proven by photoelectrochemical test and electrochemical impedance spectroscopy (EIS) analysis. Ascorbic acid was utilized as the electron donor for scavenging photo-generated holes and inhibiting light driven electron-hole pair recombination. The specific recognition between thrombin aptamer and thrombin led to the linear decrease of photocurrent with the increase of logarithm of thrombin concentration in the range of 5.0×10(-13)molL(-1) to 5.0×10(-9)molL(-1) with a detection limit of 1.2×10(-13)molL(-1). This proposed low-cost, convenient and sensitive aptasensor showed promising applications in biosensor and photoelectrochemical analysis.
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Dawei
authors: D
authors: Fan
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Cuijuan
authors: C
authors: Guo
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Hongmin
authors: H
authors: Ma
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Di
authors: D
authors: Zhao
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Yina
authors: Y
authors: Li
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: wudan791108@163.com.
authors: Dan
authors: D
authors: Wu
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Qin
authors: Q
authors: Wei
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry; D006801:Humans; D008027:Light; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D009570:Nitriles / Q000737:chemistry; D055668:Photochemical Processes; D013917:Thrombin / Q000737:chemistry / Q000302:isolation & purification*; D014025:Titanium / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D009570:Nitriles; C009495:titanium dioxide; C011206:cyanogen; D006108:Graphite; D014025:Titanium; D013917:Thrombin; D001205:Ascorbic Acid
keywords: Aptasensor; Photoelectrochemical activity; Thrombin; Visible-light; g-C(3)N(4)/TiO(2)