title: Evaluation of Downstream Regulatory Element Antagonistic Modulator Gene in Human Multinodular Goiter.
abstract: BACKGROUND DREAM (Downstream Regulatory Element Antagonistic Modulator) is a neuronal calcium sensor that was suggested to modulate TSH receptor activity and whose overexpression provokes an enlargement of the thyroid gland in transgenic mice. The aim of this study was to investigate somatic mutations and DREAM gene expression in human multinodular goiter (MNG). MATERIAL AND METHODS DNA and RNA samples were obtained from hyperplastic thyroid glands of 60 patients (54 females) with benign MNG. DREAM mutations were evaluated by PCR and direct automatic sequencing, whereas relative quantification of mRNA was performed by real-time PCR. Over- and under-expression were defined as a 2-fold increase and decrease in comparison to normal thyroid tissue, respectively. RQ M (relative quantification mean); SD (standard deviation). RESULTS DREAM expression was detected in all nodules evaluated. DREAM mRNA was overexpressed in 31.7% of MNG (RQ M=6.26; SD=5.08), whereas 53.3% and 15% had either normal (RQ M=1.16; SD=0.46) or underexpression (RQ M=0.30; SD=0.10), respectively. Regarding DREAM mutations analysis, only previously described intronic polymorphisms were observed. CONCLUSIONS We report DREAM gene expression in the hyperplastic thyroid gland of MNG patients. However, DREAM expression did not vary significantly, and was somewhat underexpressed in most patients, suggesting that DREAM upregulation does not significantly affect nodular development in human goiter.
authors: Laboratory of Cellular and Molecular Endocrinology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil.
authors: Amanda
authors: A
authors: Shinzato
authors: Laboratory of Cellular and Molecular Endocrinology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil.
authors: Antonio M
authors: AM
authors: Lerario
authors: Laboratory of Cardiovascular Pathology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil.
authors: Chin J
authors: CJ
authors: Lin
authors: Laboratory of Cellular and Molecular Endocrinology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil.
authors: Debora S
authors: DS
authors: Danilovic
authors: Laboratory of Cellular and Molecular Endocrinology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil.
authors: Suemi
authors: S
authors: Marui
authors: Laboratory of Cellular and Molecular Endocrinology, Hospital of the School of Medicine of São Paulo University (HCFMUSP), São Paulo, SP, Brazil.
authors: Ericka B
authors: EB
authors: Trarbach
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006044:Goiter, Nodular / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051605:Kv Channel-Interacting Proteins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009154:Mutation; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D055815:Young Adult
chemical_list: C496749:KCNIP3 protein, human; D051605:Kv Channel-Interacting Proteins; D012097:Repressor Proteins; D012313:RNA; D004247:DNA