title: Alphavirus vectors as tools in neuroscience and gene therapy.
abstract: Alphavirus-based vectors have been engineered for in vitro and in vivo expression of heterelogous genes. The rapid and easy generation of replication-deficient recombinant particles and the broad range of host cell infection have made alphaviruses attractive vehicles for applications in neuroscience and gene therapy. Efficient delivery to primary neurons and hippocampal slices has allowed localization studies of gene expression and electrophysiological recordings of ion channels. Alphavirus vectors have also been applied for in vivo delivery to rodent brain. Due to the strong local transient expression provided by alphavirus vectors a number of immunization and gene therapy approaches have demonstrated both therapeutic and prophylactic efficacy in various animal models.
authors: PanTherapeutics, Rue des Remparts 4, CH1095 Lutry, Switzerland. Electronic address: lundstromkenneth@gmail.com.
authors: Kenneth
authors: K
authors: Lundstrom
mesh_terms: D000524:Alphavirus / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000295:instrumentation* / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009488:Neurosciences / Q000295:instrumentation / Q000379:methods*
keywords: Alphavirus vectors; Cancer gene therapy; Cancer vaccines; Micro-RNA; Neuroscience