title: Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering.
abstract: Urinary tract infection (UTI) is a widespread infection and affects millions of people around the globe. The gold standard for identification of microorganisms causing infection is urine culture. However, current methods require at least 24 h for the results. In clinical settings, identification and discrimination of bacteria with less time-consuming and cheaper methods are highly desired. In recent years, the power of surface-enhanced Raman scattering (SERS) for fast identification of bacteria and biomolecules has been demonstrated. In this study, we show discrimination of urinary tract infection causative pathogens within 1 h of incubation using principal component analysis (PCA) of SERS spectra of seven different UTI causative bacterial species. In addition, we showed differentiation of them at their different growth phases. We also analyzed origins of bacterial SERS spectra and demonstrated the highly dynamic structure of the bacteria cell wall during their growth. Graphical Abstract Collection of bacteria from urine sample, and their discrimination using their SERS spectra and multivariate analysis.
authors: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, 34755, Turkey.
authors: Ertug
authors: E
authors: Avci
authors: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, 34755, Turkey.
authors: Nur Selin
authors: NS
authors: Kaya
authors: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, 34755, Turkey.
authors: Gizem
authors: G
authors: Ucankus
authors: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, 34755, Turkey. mculha@yeditepe.edu.tr.
authors: Mustafa
authors: M
authors: Culha
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology* / Q000652:urine*
chemical_list: D012834:Silver
keywords: Bacteria; Growth phase; Principal component analysis; Surface-enhanced Raman scattering; Urinary tract infection