title: Labeling and Tracking of Human Pancreatic Islets Using Carbon Nanotubes.
abstract: Limited tools are available for the non-invasive monitoring of transplanted islets. In this study, we have compared the widely used superparamagnetic iron oxide nanoparticle ferumoxide (Endorem) and multiwalled carbon nanotubes (MWCNTs) for islet cell labeling and tracking. INS-1 E cells and human pancreatic islets isolated from 12 non-diabetic cadaveric organ donors (age: 62 ±16 yr, BMI: 24.6 ± 3.3 kg/m2) were incubated with 50 μg/ml Endorem or 15 μg/ml MWCNTs and studied after 7 or 14 days to assess beta cell morphology, ultrastructure, function, cell survival and in-vitro and in-vivo magnetic resonance imaging (MRI). Light and electron (EM) microscopy showed the well-maintained morphology and ultrastructure of both INS-1 E and human islets during the incubation. EM also revealed the presence of Endorem and MWCNTs within the beta but not the alpha cells. The compounds did not affect beta cell function and viability, and in-vitro MRI showed that labeled INS-1 E cells and human islets could be imaged. Finally, MWCNT labeled human islets were successfully transplanted into the subcutis of rats localized in the desired site via magnetic field and tracked by MRI. These data suggest that MWCNTs can be an alternative labeling compound to be used with human islets for experimental and transplantation studies.
authors: Farooq
authors: F
authors: Syed
authors: Cristina
authors: C
authors: Riggio
authors: Matilde
authors: M
authors: Masini
authors: Marco
authors: M
authors: Bugliani
authors: Valentina
authors: V
authors: Battaglia
authors: Michela
authors: M
authors: Novelli
authors: Mara
authors: M
authors: Suleiman
authors: Orazio
authors: O
authors: Vittorio
authors: Ugo
authors: U
authors: Boggi
authors: Franco
authors: F
authors: Filipponi
authors: Lorella
authors: L
authors: Marselli
authors: Carlo
authors: C
authors: Bartolozzi
authors: Pellegrino
authors: P
authors: Masiello
authors: Vittoria
authors: V
authors: Raffa
authors: Piero
authors: P
authors: Marchetti
mesh_terms: D000368:Aged; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D051381:Rats
chemical_list: D003287:Contrast Media; D003911:Dextrans; D007328:Insulin; D058185:Magnetite Nanoparticles; D037742:Nanotubes, Carbon; C065507:ferumoxides