title: Engineering Photosystem I Complexes with Metal Oxide Binding Peptides for Bioelectronic Applications.
abstract: Conventional dye-sensitized solar cells comprise semiconducting anodes sensitized with complex synthetic organometallic dyes, a platinum counter electrode, and a liquid electrolyte. This work focuses on replacing synthetic dyes with a naturally occurring biological pigment-protein complex known as Photosystem I (PSI). Specifically, ZnO binding peptides (ZOBiP)-fused PSI subunits (ZOBiP-PsaD and ZOBiP-PsaE) and TiO2 binding peptides (TOBiP)-fused ferredoxin (TOBiP-Fd) have been produced recombinantly from Escherichia coli. The MOBiP-fused peptides have been characterized via western blotting, circular dichroism, MALDI-TOF, and cyclic voltammetry. ZOBiP-PSI subunits have been used to replace wild-type PsaD and PsaE, and TOBiP-Fd has been chemically cross-linked to the stromal hump of PSI. These MOBiP peptides and MOBiP-PSI complexes have been produced and incubated with various metal oxide nanoparticles, showing increased binding when compared to that of wild-type PSI complexes.
authors: Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee Knoxville , Knoxville, Tennessee 37919, United States.
authors: Richard F
authors: RF
authors: Simmerman
authors: Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee Knoxville , Knoxville, Tennessee 37919, United States.
authors: Tuo
authors: T
authors: Zhu
authors: Sensors and Electron Devices Directorate, U.S. Army Research Laboratory , Adelphi, Maryland 20783, United States.
authors: David R
authors: DR
authors: Baker
authors: Department of Physics, University of Memphis , Memphis, Tennessee 38152, United States.
authors: Lijia
authors: L
authors: Wang
authors: Department of Physics, University of Memphis , Memphis, Tennessee 38152, United States.
authors: Sanjay R
authors: SR
authors: Mishra
authors: Sensors and Electron Devices Directorate, U.S. Army Research Laboratory , Adelphi, Maryland 20783, United States.
authors: Cynthia A
authors: CA
authors: Lundgren
authors: Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee Knoxville , Knoxville, Tennessee 37919, United States.
authors: Barry D
authors: BD
authors: Bruce
mesh_terms: D001674:Bioelectric Energy Sources; D015153:Blotting, Western; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D012666:Semiconductors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium / Q000378:metabolism; D015034:Zinc Oxide / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D010455:Peptides; D045331:Photosystem I Protein Complex; D011993:Recombinant Fusion Proteins; C079741:photosystem I, psaE subunit; C009495:titanium dioxide; D014025:Titanium; D015034:Zinc Oxide