title: Fast internal dynamics in alcohol dehydrogenase.
abstract: Large-scale domain motions in alcohol dehydrogenase (ADH) have been observed previously by neutron spin-echo spectroscopy (NSE). We have extended the investigation on the dynamics of ADH in solution by using high-resolution neutron time-of-flight (TOF) and neutron backscattering (BS) spectroscopy in the incoherent scattering range. The observed hydrogen dynamics were interpreted in terms of three mobility classes, which allowed a simultaneous description of the measured TOF and BS spectra. In addition to the slow global protein diffusion and domain motions observed by NSE, a fast internal process could be identified. Around one third of the protons in ADH participate in the fast localized diffusive motion. The diffusion coefficient of the fast internal motions is around two third of the value of the surrounding D2O solvent. It is tempting to associate the fast internal process with solvent exposed amino acid residues with dangling side chains.
authors: Jülich Centre for Neutron Science JCNS and Institute for Complex Systems ICS, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
authors: M
authors: M
authors: Monkenbusch
authors: Jülich Centre for Neutron Science JCNS and Institute for Complex Systems ICS, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
authors: A
authors: A
authors: Stadler
authors: Jülich Centre for Neutron Science JCNS and Institute for Complex Systems ICS, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
authors: R
authors: R
authors: Biehl
authors: Institut Laue-Langevin, CS 20156, 38042 Grenoble, France.
authors: J
authors: J
authors: Ollivier
authors: Jülich Centre for Neutron Science JCNS, Forschungszentrum Jülich GmbH, Outstation at MLZ, Lichtenbergstraße 1, 85747 Garching, Germany.
authors: M
authors: M
authors: Zamponi
authors: Jülich Centre for Neutron Science JCNS and Institute for Complex Systems ICS, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
authors: D
authors: D
authors: Richter
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D017666:Deuterium Oxide / Q000737:chemistry; D004058:Diffusion; D000067493:Dynamic Light Scattering; D006859:Hydrogen / Q000737:chemistry; D008958:Models, Molecular; D009038:Motion; D033363:Neutron Diffraction; D011522:Protons; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D011522:Protons; D012996:Solutions; D012997:Solvents; D006859:Hydrogen; D000426:Alcohol Dehydrogenase; D017666:Deuterium Oxide