title: Exome Sequencing of Cell-Free DNA from Metastatic Cancer Patients Identifies Clinically Actionable Mutations Distinct from Primary Disease.
abstract: The identification of the molecular drivers of cancer by sequencing is the backbone of precision medicine and the basis of personalized therapy; however, biopsies of primary tumors provide only a snapshot of the evolution of the disease and may miss potential therapeutic targets, especially in the metastatic setting. A liquid biopsy, in the form of cell-free DNA (cfDNA) sequencing, has the potential to capture the inter- and intra-tumoral heterogeneity present in metastatic disease, and, through serial blood draws, track the evolution of the tumor genome. In order to determine the clinical utility of cfDNA sequencing we performed whole-exome sequencing on cfDNA and tumor DNA from two patients with metastatic disease; only minor modifications to our sequencing and analysis pipelines were required for sequencing and mutation calling of cfDNA. The first patient had metastatic sarcoma and 47 of 48 mutations present in the primary tumor were also found in the cell-free DNA. The second patient had metastatic breast cancer and sequencing identified an ESR1 mutation in the cfDNA and metastatic site, but not in the primary tumor. This likely explains tumor progression on Anastrozole. Significant heterogeneity between the primary and metastatic tumors, with cfDNA reflecting the metastases, suggested separation from the primary lesion early in tumor evolution. This is best illustrated by an activating PIK3CA mutation (H1047R) which was clonal in the primary tumor, but completely absent from either the metastasis or cfDNA. Here we show that cfDNA sequencing supplies clinically actionable information with minimal risks compared to metastatic biopsies. This study demonstrates the utility of whole-exome sequencing of cell-free DNA from patients with metastatic disease. cfDNA sequencing identified an ESR1 mutation, potentially explaining a patient's resistance to aromatase inhibition, and gave insight into how metastatic lesions differ from the primary tumor.
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Timothy M
authors: TM
authors: Butler
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Katherine
authors: K
authors: Johnson-Camacho
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Myron
authors: M
authors: Peto
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Nicholas J
authors: NJ
authors: Wang
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Tara A
authors: TA
authors: Macey
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: James E
authors: JE
authors: Korkola
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Theresa M
authors: TM
authors: Koppie
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Christopher L
authors: CL
authors: Corless
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Joe W
authors: JW
authors: Gray
authors: Knight Cancer Institute, Oregon Health and Sciences University, Portland, Oregon, United States of America.
authors: Paul T
authors: PT
authors: Spellman
mesh_terms: D019943:Amino Acid Substitution; D058534:Class I Phosphatidylinositol 3-Kinases; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D059472:Exome; D005260:Female; D006801:Humans; D008875:Middle Aged; D020125:Mutation, Missense; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D012509:Sarcoma / Q000097:blood / Q000235:genetics* / Q000473:pathology
chemical_list: D058534:Class I Phosphatidylinositol 3-Kinases; D004273:DNA, Neoplasm; D047628:Estrogen Receptor alpha; D009363:Neoplasm Proteins; D019869:Phosphatidylinositol 3-Kinases; C506487:ESR1 protein, human; C484760:PIK3CA protein, human