title: Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS).
abstract: A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0ng/mL for each analyte in plasma and 3.0-1500.0ng/g in brain tissue. The method was validated with precision within 15% relative standard deviation (RSD), accuracy within 15% relative error (RE), matrix effects within 10% and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.
authors: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA 30602-2352, United States.
authors: Xiangkun
authors: X
authors: Yang
authors: Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, GA 30912, United States.
authors: Indrani
authors: I
authors: Poddar
authors: Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, GA 30912, United States.
authors: Caterina M
authors: CM
authors: Hernandez
authors: Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, GA 30912, United States.
authors: Alvin V
authors: AV
authors: Terry
authors: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA 30602-2352, United States. Electronic address: bartlett@rx.uga.edu.
authors: Michael G
authors: MG
authors: Bartlett
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D000069348:Quetiapine Fumarate / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000069348:Quetiapine Fumarate
keywords: Brain; LC-MS/MS; Norquetiapine; Plasma; Quetiapine