title: Transition toward Human Cytomegalovirus Susceptibility in Early Human Embryonic Stem Cell-Derived Neural Precursors.
abstract: Congenital human cytomegalovirus (HCMV) infection is associated with neurodevelopmental disabilities. To dissect the earliest events of infection in the developing human brain, we studied HCMV infection during controlled differentiation of human embryonic stem cells (hESC) into neural precursors. We traced a transition from viral restriction in hESC, mediated by a block in viral binding, toward HCMV susceptibility in early hESC-derived neural precursors. We further revealed the role of platelet-derived growth factor receptor alpha (PDGFRα) as a determinant of the developmentally acquired HCMV susceptibility.
authors: Clinical Virology Unit, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel Department of Biochemistry and the Chanock Center for Virology, IMRIC, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Amnon A
authors: AA
authors: Berger
authors: The Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy and Department of Gynecology, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Yaniv
authors: Y
authors: Gil
authors: Department of Biochemistry and the Chanock Center for Virology, IMRIC, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Amos
authors: A
authors: Panet
authors: Clinical Virology Unit, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel Department of Biochemistry and the Chanock Center for Virology, IMRIC, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Yiska
authors: Y
authors: Weisblum
authors: Clinical Virology Unit, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel Department of Biochemistry and the Chanock Center for Virology, IMRIC, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Esther
authors: E
authors: Oiknine-Djian
authors: The Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy and Department of Gynecology, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Michal
authors: M
authors: Gropp
authors: The Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy and Department of Gynecology, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel.
authors: Debora
authors: D
authors: Steiner
authors: The Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy and Department of Gynecology, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel benr@hadassah.org.il dana.wolf@ekmd.huji.ac.il.
authors: Benjamin E
authors: BE
authors: Reubinoff
authors: Clinical Virology Unit, Hadassah Hebrew University Medical Center and Faculty of Medicine, Jerusalem, Israel benr@hadassah.org.il dana.wolf@ekmd.huji.ac.il.
authors: Dana G
authors: DG
authors: Wolf
mesh_terms: D000367:Age Factors; D002454:Cell Differentiation / Q000502:physiology*; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000503:physiopathology* / Q000517:prevention & control; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D058953:Neural Stem Cells / Q000502:physiology / Q000821:virology*; D053585:Virus Attachment