title: Comparative study of nanosized cross-linked sodium-, linear sodium- and zinc-hyaluronate as potential ocular mucoadhesive drug delivery systems.
abstract: Hyaluronic acid (HA) and its derivatives play important roles in many fields of therapy, such as arthritis treatment, plastic surgery, dermatology, otology, ophthalmology, etc. With a view to increase the beneficial properties of HA in ocular drug delivery, many types of chemical structural modifications have been performed. In the course of our research work, we characterized nanosized cross-linked - (CLNaHA), linear sodium hyaluronate (NaHA) and zinc-hyaluronate (ZnHA), as potential ocular drug delivery systems. The aim was to determine the influence of the structure on biocompatibility, mucoadhesion and drug release. The structure was characterized by means of rheology. The cytotoxicity of the samples was determined on rabbit corneal epithelial cells (RCE) by the MTT test. Mucoadhesion measurements were made by a rheological method in vitro and by tensile tests in vitro and ex vivo. The release of sodium diclofenac, a frequently used non-steroidal anti-inflammatory drug with low bioavailability, from the gels was determined with a vertical Franz diffusion cell. The results demonstrated that all three derivatives have adequate mucoadhesive properties and their rapid drug release profiles are beneficial in ocular therapy. Thanks to these properties, the bioavailability of the ophthalmic preparations can be increased, especially with the application of CLNaHA.
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary.
authors: Gabriella
authors: G
authors: Horvát
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary.
authors: Mária
authors: M
authors: Budai-Szűcs
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary.
authors: Szilvia
authors: S
authors: Berkó
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary.
authors: Piroska
authors: P
authors: Szabó-Révész
authors: Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, H-6720 Szeged, Hungary.
authors: Judit
authors: J
authors: Soós
authors: Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, H-6720 Szeged, Hungary.
authors: Andrea
authors: A
authors: Facskó
authors: TEVA Pharmaceuticals Ltd, Pallagi u. 13, H-4042 Debrecen, Hungary.
authors: Mónika
authors: M
authors: Maroda
authors: Department of Drug Sciences, Faculty of Pharmacy, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors: Michela
authors: M
authors: Mori
authors: Department of Drug Sciences, Faculty of Pharmacy, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors: Giuseppina
authors: G
authors: Sandri
authors: Department of Drug Sciences, Faculty of Pharmacy, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors: Maria Cristina
authors: MC
authors: Bonferoni
authors: Department of Drug Sciences, Faculty of Pharmacy, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors: Carla
authors: C
authors: Caramella
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary. Electronic address: csanyi@pharm.u-szeged.hu.
authors: Erzsébet
authors: E
authors: Csányi
mesh_terms: D000269:Adhesives / Q000009:adverse effects / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry; D001682:Biological Availability; D002470:Cell Survival / Q000187:drug effects; D004008:Diclofenac / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000009:adverse effects / Q000379:methods*; D065546:Drug Liberation; D019573:Epithelium, Corneal / Q000187:drug effects; D005782:Gels / Q000009:adverse effects / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000009:adverse effects / Q000737:chemistry*; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D009942:Organometallic Compounds / Q000009:adverse effects / Q000737:chemistry*; D011817:Rabbits; D012212:Rheology
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D005782:Gels; D009942:Organometallic Compounds; C112514:zinc hyaluronate complex; D004008:Diclofenac; D006820:Hyaluronic Acid
keywords: Cross-linked sodium hyaluronate; Cytotoxicity; Hyaluronic acid; Mucoadhesion; Ocular drug release; Zinc hyaluronate