title: Hsp70 plays an important role in high-fat diet induced gestational hyperglycemia in mice.
abstract: Gestational diabetes mellitus (GDM) has emerged as an epidemic disease during the last decade, affecting about 2 to 5% pregnant women. Even among women who have gestational hyperglycemia may also be positively related to adverse outcomes as GDM. Since heat shock protein (Hsp) 70 has been reported to be associated with diabetes and insulin resistance and its expression was reported to be negatively regulated by the membrane-permeable Hsp70 inhibitor MAL3-101 while positively regulated by the Hsp70 activator BGP-15, we investigated whether Hsp70 played a role in a gestational hyperglycemia mouse model. Mice were divided into non-pregnant and pregnant groups, and each comprised three subgroups: control, high-fat diet (HFD) + MAL3-101, and HFD + BGP-15. We examined the serum levels of triglycerides, total cholesterol, glucose, and insulin, as well as conducted thermal detection of brown adipose tissue (BAT). The role of Hsp70 in BAT apoptosis was also investigated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and caspase-3 staining. Higher serum level of Hsp70 was associated with increased bodyweight gain after pregnancy in mice fed HFD. Circulating Hsp70 was elevated in control pregnant mice compared to control non-pregnant mice. BGP-induced serum Hsp70 expression reduced triglycerides, total cholesterol, glucose, and insulin levels in the serum. Additionally, thermal detection of BAT, TUNEL, and caspase-3 staining revealed relationship correlation between Hsp70 and BAT functions. Hsp70 level is associated with hyperglycemia during pregnancy. Our results support the role of Hsp70 in facilitating BAT activities and protecting BAT cells from apoptosis via caspase-3 pathway.
authors: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou, 061001, China. xbh08@126.com.
authors: Baoheng
authors: B
authors: Xing
authors: Department of Neurology, Cangzhou City People's Hospital, Cangzhou, 061000, China.
authors: Lili
authors: L
authors: Wang
authors: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou, 061001, China.
authors: Qin
authors: Q
authors: Li
authors: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou, 061001, China.
authors: Yalei
authors: Y
authors: Cao
authors: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou, 061001, China.
authors: Xiujuan
authors: X
authors: Dong
authors: Department of Gynecology and Obstetrics, Bethune International Peace Hospital, Zhongshan West Road, Shijiazhuang, 050051, People's Republic of China.
authors: Jun
authors: J
authors: Liang
authors: Department of Gynecology and Obstetrics, Bethune International Peace Hospital, Zhongshan West Road, Shijiazhuang, 050051, People's Republic of China. xhwu23@163.com.
authors: Xiaohua
authors: X
authors: Wu
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D001786:Blood Glucose; D016640:Diabetes, Gestational / Q000209:etiology / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D007328:Insulin / Q000097:blood; D008297:Male; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D022722:Thermogenesis
chemical_list: D001786:Blood Glucose; D018840:HSP70 Heat-Shock Proteins; D007328:Insulin
keywords: Apoptosis; Brown adipose tissue; Gestational hyperglycemia; Hsp70; Insulin resistance