title: A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia.
abstract: Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America; Department of Chemistry, Rice University, Houston, TX, United States of America; Department of Surgical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX, United States of America.
authors: Stuart J
authors: SJ
authors: Corr
authors: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America; Department of Biomedical Engineering, University of Houston, TX, United States of America.
authors: Sabeel
authors: S
authors: Shamsudeen
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America.
authors: Leoncio A
authors: LA
authors: Vergara
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America.
authors: Jason Chak-Shing
authors: JC
authors: Ho
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America.
authors: Matthew J
authors: MJ
authors: Ware
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America.
authors: Vazrik
authors: V
authors: Keshishian
authors: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America.
authors: Kenji
authors: K
authors: Yokoi
authors: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America.
authors: David J
authors: DJ
authors: Savage
authors: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America.
authors: Ismail M
authors: IM
authors: Meraz
authors: Department of Surgical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX, United States of America.
authors: Warna
authors: W
authors: Kaluarachchi
authors: Department of Chemistry, Rice University, Houston, TX, United States of America; Department of Surgical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX, United States of America.
authors: Brandon T
authors: BT
authors: Cisneros
authors: Department of Surgical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX, United States of America.
authors: Mustafa
authors: M
authors: Raoof
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America; Department of Biomedical Engineering, University of Houston, TX, United States of America.
authors: Duy Trac
authors: DT
authors: Nguyen
authors: Department of Biomedical Engineering, University of Houston, TX, United States of America.
authors: Yingchun
authors: Y
authors: Zhang
authors: Department of Chemistry, Rice University, Houston, TX, United States of America.
authors: Lon J
authors: LJ
authors: Wilson
authors: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America; Centre for Nanohealth, College of Engineering, Swansea University, Swansea, Wales, United Kingdom.
authors: Huw
authors: H
authors: Summers
authors: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America; Centre for Nanohealth, College of Engineering, Swansea University, Swansea, Wales, United Kingdom; The Broad Institute, Cambridge, MA, United States of America.
authors: Paul
authors: P
authors: Rees
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America; Department of Mechanical Engineering and Materials Science, Rice University, Houston, TX, United States of America.
authors: Steven A
authors: SA
authors: Curley
authors: Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, United States of America; Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United States of America.
authors: Rita E
authors: RE
authors: Serda
mesh_terms: D000465:Algorithms; D000818:Animals; D003952:Diagnostic Imaging; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006979:Hyperthermia, Induced; D000069416:Intravital Microscopy / Q000379:methods*; D015674:Mammary Neoplasms, Animal / Q000473:pathology* / Q000628:therapy; D051379:Mice; D011846:Radio Waves; D014018:Tissue Distribution
chemical_list: D005456:Fluorescent Dyes