title: Comparative Microarray Analysis Identifies Commonalities in Neuronal Injury: Evidence for Oxidative Stress, Dysfunction of Calcium Signalling, and Inhibition of Autophagy-Lysosomal Pathway.
abstract: Mitochondrial dysfunction, ubiquitin-proteasomal system impairment and excitotoxicity occur during the injury and death of neurons in neurodegenerative conditions. The aim of this work was to elucidate the cellular mechanisms that are universally altered by these conditions. Through overlapping expression profiles of rotenone-, lactacystin- and N-methyl-D-aspartate-treated cortical neurons, we have identified three affected biological processes that are commonly affected; oxidative stress, dysfunction of calcium signalling and inhibition of the autophagic-lysosomal pathway. These data provides many opportunities for therapeutic intervention in neurodegenerative conditions, where mitochondrial dysfunction, proteasomal inhibition and excitotoxicity are evident.
authors: Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, Burwood, VIC, 3125, Australia.
authors: Yann Wan
authors: YW
authors: Yap
authors: Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, Burwood, VIC, 3125, Australia.
authors: Roxana M
authors: RM
authors: Llanos
authors: Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, Burwood, VIC, 3125, Australia.|Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia.
authors: Sharon
authors: S
authors: La Fontaine
authors: Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, Burwood, VIC, 3125, Australia.
authors: Michael A
authors: MA
authors: Cater
authors: Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia.
authors: Philip M
authors: PM
authors: Beart
authors: Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, Burwood, VIC, 3125, Australia. steve.cheung@deakin.edu.au.
authors: Nam Sang
authors: NS
authors: Cheung
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000633:toxicity; D000818:Animals; D001343:Autophagy; D020013:Calcium Signaling; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D046228:Microarray Analysis; D019636:Neurodegenerative Diseases / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress; D010575:Pesticides / Q000633:toxicity; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D061988:Proteasome Inhibitors / Q000633:toxicity; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D010575:Pesticides; D061988:Proteasome Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; D025801:Ubiquitin; D012402:Rotenone; C067713:lactacystin; D046988:Proteasome Endopeptidase Complex; D000111:Acetylcysteine
keywords: Lactacystin; NMDA; Programmed cell death; Rotenone