title: Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry.
abstract: BACKGROUND
  Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices.
  
  
  METHODS
  An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A.
  
  
  RESULTS
  Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL.
  
  
  CONCLUSIONS
  This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening.
authors: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, China.
authors: Hongliu
authors: H
authors: Sun
authors: Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, #3, Beijing,China.
authors: Cai
authors: C
authors: Qi
authors: Institute of Mechanics, Chinese Academy of Sciences, #15, Beijing, China.
authors: Yu
authors: Y
authors: Niu
authors: Institute of Mechanics, Chinese Academy of Sciences, #15, Beijing, China.
authors: Tengfei
authors: T
authors: Kang
authors: Food Laboratory, Beijing Inspection and Quarantine Testing Center, #6, Beijing, China.
authors: Yongxin
authors: Y
authors: Wei
authors: Institute of Mechanics, Chinese Academy of Sciences, #15, Beijing, China.
authors: Gang
authors: G
authors: Jin
authors: Institute of Biophysics, Chinese Academy of Sciences, #15, Beijing, China.
authors: Xianzhi
authors: X
authors: Dong
authors: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, China.
authors: Chunhua
authors: C
authors: Wang
authors: Institute of Radiation Medicine, Shandong Academy of Medical Sciences, #18877, Jinan, China.
authors: Wei
authors: W
authors: Zhu
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D015374:Biosensing Techniques / Q000379:methods*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D046210:Microfluidic Analytical Techniques / Q000379:methods; D055096:Optical Devices; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M