title: Ultraporous nanofeatured PCL-PEO microfibrous scaffolds enhance cell infiltration, colonization and myofibroblastic differentiation.
abstract: In the field of tissue engineering, integration of micro-porosity, nano-topogaphical features and weattability into one three-dimensional (3D) scaffold remains a challenge. The extracellular matrix (ECM) mimicking feature of electrospun fibers endows them wide applications in tissue engineering. However, the tight-packing of electrospun submicron fibers hinder cell infiltration and further colonization. In this study, we fabricated hydrophilic, micro-porous scaffolds with nano-topographical cues by one-step electrospinning, and investigated NIH3T3 fibroblasts cell infiltration, colonization and myofibroblastic differentiation. The hierarchical porosity enhanced cell infiltration and proliferation significantly. Besides, the nano-topography influenced the cell actin distribution and cell morphology that stimulated myofibroblastic differentiation in a drastically different manner from that of traditional solid, smooth electrospun fibers, which may hold great potential in reconstructing tissues that require strong contractile forces.
authors: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark. menglin@inano.au.dk.
authors: Yan-Fang
authors: YF
authors: Li
authors: Hans
authors: H
authors: Gregersen
authors: Jens Vinge
authors: JV
authors: Nygaard
authors: Weilu
authors: W
authors: Cheng
authors: Ying
authors: Y
authors: Yu
authors: Yudong
authors: Y
authors: Huang
authors: Mingdong
authors: M
authors: Dong
authors: Flemming
authors: F
authors: Besenbacher
authors: Menglin
authors: M
authors: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005109:Extracellular Matrix / Q000737:chemistry*; D051379:Mice; D058628:Myofibroblasts / Q000166:cytology / Q000378:metabolism*; D041681:NIH 3T3 Cells; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*