title: PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain.
abstract: Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure.
authors: Research and Development Section, Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Ontario, Canada K1A 0Y9.
authors: Michael
authors: M
authors: Knowles
authors: Research and Development Section, Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Ontario, Canada K1A 0Y9.
authors: Dominic
authors: D
authors: Lambert
authors: Research and Development Section, Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Ontario, Canada K1A 0Y9.
authors: George
authors: G
authors: Huszczynski
authors: Research and Development Section, Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Ontario, Canada K1A 0Y9.
authors: Martine
authors: M
authors: Gauthier
authors: Research and Development Section, Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Ontario, Canada K1A 0Y9. burton.blais@inspection.gc.ca.
authors: Burton W
authors: BW
authors: Blais
mesh_terms: D019295:Computational Biology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D016680:Genome, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial