title: Proteomic analysis of cellular response induced by boron neutron capture reaction in human squamous cell carcinoma SAS cells.
abstract: To understand the mechanism of cell death induced by boron neutron capture reaction (BNCR), we performed proteome analyses of human squamous tumor SAS cells after BNCR. Cells were irradiated with thermal neutron beam at KUR after incubation under boronophenylalanine (BPA)(+) and BPA(-) conditions. BNCR mainly induced typical apoptosis in SAS cells 24h post-irradiation. Proteomic analysis in SAS cells suggested that proteins functioning in endoplasmic reticulum, DNA repair, and RNA processing showed dynamic changes at early phase after BNCR and could be involved in the regulation of cellular response to BNCR. We found that the BNCR induces fragments of endoplasmic reticulum-localized lymphoid-restricted protein (LRMP). The fragmentation of LRMP was also observed in the rat tumor graft model 20 hours after BNCT treatment carried out at the National Nuclear Center of the Republic of Kazakhstan. These data suggest that dynamic changes of LRMP could be involved during cellular response to BNCR.
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Japan.
authors: Akira
authors: A
authors: Sato
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Japan; Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Japan.
authors: Tasuku
authors: T
authors: Itoh
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Japan.
authors: Shoji
authors: S
authors: Imamichi
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Japan; Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Japan.
authors: Sota
authors: S
authors: Kikuhara
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Japan.
authors: Hiroaki
authors: H
authors: Fujimori
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Japan; Department of Radiation Oncology, Juntendo University, Faculty of Medicine, Japan.
authors: Takahisa
authors: T
authors: Hirai
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan.
authors: Soichiro
authors: S
authors: Saito
authors: Research Reactor Institute, Kyoto University, Japan.
authors: Yoshinori
authors: Y
authors: Sakurai
authors: Research Reactor Institute, Kyoto University, Japan.
authors: Hiroki
authors: H
authors: Tanaka
authors: Chemical Resources Laboratory, Tokyo Institute of Technology, Japan.
authors: Hiroyuki
authors: H
authors: Nakamura
authors: Research Reactor Institute, Kyoto University, Japan.
authors: Minoru
authors: M
authors: Suzuki
authors: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Japan.
authors: Yasufumi
authors: Y
authors: Murakami
authors: Centre for Life Sciences, Nazarbayev University, Kazakhstan.
authors: Diaz
authors: D
authors: Baiseitov
authors: Centre for Life Sciences, Nazarbayev University, Kazakhstan.
authors: Kulzhan
authors: K
authors: Berikkhanova
authors: Centre for Life Sciences, Nazarbayev University, Kazakhstan.
authors: Zhaxybay
authors: Z
authors: Zhumadilov
authors: Cancer Intelligence Care Systems, Inc., Life Sciences Center, Japan.
authors: Yoshio
authors: Y
authors: Imahori
authors: Department of Radiation Oncology, National Cancer Center Hospital, Japan.
authors: Jun
authors: J
authors: Itami
authors: Research Reactor Institute, Kyoto University, Japan.
authors: Koji
authors: K
authors: Ono
authors: Research Reactor Institute, Kyoto University, Japan.
authors: Shinichiro
authors: S
authors: Masunaga
authors: Division of Genome Stability Research, National Cancer Center Research Institute, Japan; Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Japan; Department of Frontier Life Sciences, Nagasaki University Graduate School of Biomedical Sciences, Japan; Central Radioisotope Division, National Cancer Center Research Institute, Japan. Electronic address: mmasutan@ncc.go.jp.
authors: Mitsuko
authors: M
authors: Masutani
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D016754:Boron Neutron Capture Therapy; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D009363:Neoplasm Proteins / Q000378:metabolism*; D040901:Proteomics
chemical_list: D009363:Neoplasm Proteins
keywords: Apoptosis; Boron neutron capture reaction (BNCR); Lymphoid-restricted protein (LRMP); Proteome analysis