title: Chemopreventive effects of Strobilanthes crispus leaf extract on azoxymethane-induced aberrant crypt foci in rat colon.
abstract: In this work, microscopic and histological studies suggest that Strobilanthes crispus ethanol extract reduce azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. S. crispus is considered a traditional medicine and used as an antioxidant. Its leaf contains a large amount of phenolic compounds to which its radical scavenging role is attributed and enhance its ability to eradicate oxidative stress reactions. The study was designed to determine the chemopreventive effect of S. crispus ethanol extract in vivo and in vitro by elucidating the effect of the extract on intermediate biomarkers which can be used as effective predictors of colon cancer. S. crispus was analyzed for DPPH free radical scavenging, nitric oxide (NO) and ferric acid reduction. The results indicated that S. crispus oral administration significantly inhibited colorectal carcinogenesis induced by AOM as revealed by the reduction in the number of ACF. S. crispus down-regulated the expression of PCNA, Bcl2 and β-catenin. Additionally, it exerted a pronounced inhibitory effect on MDA and NO levels and stimulatory effect on CAT and GPx activities. These results demonstrate that S. crispus is a chemopreventive agent for colorectal cancer through the suppression of early and intermediate carcinogenic phases that may be related to its flavonoid content.
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur , Malaysia.
authors: Nawal
authors: N
authors: Al-Henhena
authors: Department of Experimental Hematology, Karolinska University Hospital, SE-141 86 Stockholm, Sweden.
authors: Shaden A M
authors: SA
authors: Khalifa
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur , Malaysia.
authors: Rozaida Poh Yuen
authors: RP
authors: Ying
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur , Malaysia.
authors: Pouya
authors: P
authors: Hassandarvish
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur , Malaysia.
authors: Elham
authors: E
authors: Rouhollahi
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur , Malaysia.
authors: Nahla Saeed
authors: NS
authors: Al-Wajeeh
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Habibah Mohd
authors: HM
authors: Ali
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur , Malaysia.
authors: Mahmood Ameen
authors: MA
authors: Abdulla
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.|Department of Experimental Hematology, Karolinska University Hospital, SE-141 86 Stockholm, Sweden.|Division of Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, Box 574, SE-75 123, Uppsala, Sweden.
authors: Hesham R
authors: HR
authors: El-Seedi
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001397:Azoxymethane; D002273:Carcinogens; D018890:Chemoprevention / Q000379:methods; D003106:Colon / Q000187:drug effects*; D003111:Colonic Polyps / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D001397:Azoxymethane