title: Bacteriophage-based nanoprobes for rapid bacteria separation.
abstract: The lack of practical methods for bacterial separation remains a hindrance for the low-cost and successful development of rapid detection methods from complex samples. Antibody-tagged magnetic particles are commonly used to pull analytes from a liquid sample. While this method is well-established, improvements in capture efficiencies would result in an increase of the overall detection assay performance. Bacteriophages represent a low-cost and more consistent biorecognition element as compared to antibodies. We have developed nanoscale bacteriophage-tagged magnetic probes, where T7 bacteriophages were bound to magnetic nanoparticles. The nanoprobe allowed the specific recognition and attachment to E. coli cells. The phage magnetic nanprobes were directly compared to antibody-conjugated magnetic nanoprobes. The capture efficiencies of bacteriophages and antibodies on nanoparticles for the separation of E. coli K12 at varying concentrations were determined. The results indicated a similar bacteria capture efficiency between the two nanoprobes.
authors: Department of Food Science, University of Massachusetts, Amherst, 102 Holdsworth Way, Amherst, Massachusetts 01003, USA. snugen@umass.edu.
authors: Juhong
authors: J
authors: Chen
authors: Bradley
authors: B
authors: Duncan
authors: Ziyuan
authors: Z
authors: Wang
authors: Li-Sheng
authors: LS
authors: Wang
authors: Vincent M
authors: VM
authors: Rotello
authors: Sam R
authors: SR
authors: Nugen
mesh_terms: D017123:Bacteriophage T7 / Q000737:chemistry* / Q000648:ultrastructure; D048168:Escherichia coli K12 / Q000166:cytology* / Q000648:ultrastructure; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D058185:Magnetite Nanoparticles