title: 'Candidatus Liberibacter solanacearum' Titers in and Infection Effects on Potato Tuber Chemistry of Promising Germplasm Exhibiting Tolerance to Zebra Chip Disease.
abstract: Long-term sustainable management of zebra chip (ZC) disease of potato requires development of tolerant or resistant germplasm. To this end, 283 potato varieties and breeding clones were infected with the ZC putative causal agent 'Candidatus Liberibacter solanacearum' (Lso) by potato psyllid vector inoculations in 2010, 2011, 2012, and 2013. Potato germplasm was then examined for development of fresh and fried ZC symptoms. Over multiple years 29 breeding clones exhibited little to no symptoms in freshly cut tuber slices, and five exhibited little to no symptoms in fried slices. These five presumed tolerant breeding clones were chosen for further screening to determine whether the lack of physiological responses to Lso infection was the cause of observed tolerance. To this end, tuber amino acid, sugar, and phenolic levels were compared between noninfected and Lso-infected plants. The five putative tolerant clones had less dramatic shifts in host physiology following Lso infection than the susceptible Atlantic cultivar. This suggested lack of host responses to Lso infection that result in major changes in tuber biochemistry is a potential mechanism of ZC resistance. However, the susceptible Atlantic cultivar did have consistently greater Lso titers compared with two of the tolerant entries, so for these reductions in Lso pathogen progression also might be a factor. Regardless, lack of host responses could still remain one trait that could be used to aid in selection of ZC-resistant potato varieties, as other tolerant lines had infection levels consistent with susceptible Atlantic cultivar. These results also suggest that germplasm derived from relatives of cultivated potato plants are viable sources of ZC disease resistance.
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: C M
authors: CM
authors: Wallis
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: J E
authors: JE
authors: Munyaneza
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: J
authors: J
authors: Chen
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: R
authors: R
authors: Novy
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: G
authors: G
authors: Bester
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: J L
authors: JL
authors: Buchman
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: J
authors: J
authors: Nordgaard
authors: First and third authors: U.S. Department of Agriculture-Agriculture Research Services (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Research Unit, 9611 S. Riverbend Ave., Parlier, CA 93648; second author: USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951; fourth author: USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID 83210; fifth author: Frito-Lay Inc., 4295 Tenderfoot Road, Rhinelander, WI 54501; sixth and seventh authors: Black Gold Farms, 4320 18th Ave. S., Grand Forks, ND 58201; and eighth author: Bejo Seeds Inc., 1972 Silver Spur Place, Oceano, CA 93445.
authors: P
authors: P
authors: van Hest
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D006790:Host-Parasite Interactions; D010636:Phenols / Q000378:metabolism; D010935:Plant Diseases; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D000596:Amino Acids; D010636:Phenols
keywords: carbohydrates; phenolics; plant defense responses; terpenoids; tomato−potato psyllid