title: Comparisons of Differentiation Potential in Human Mesenchymal Stem Cells from Wharton's Jelly, Bone Marrow, and Pancreatic Tissues.
abstract: Background. Type 1 diabetes mellitus results from autoimmune destruction of β-cells. Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) in human tissues decrease blood glucose levels and improve survival in diabetic rats. We compared the differential ability and the curative effect of IPCs from three types of human tissue to determine the ideal source of cell therapy for diabetes. Methods. We induced MSCs from Wharton's jelly (WJ), bone marrow (BM), and surgically resected pancreatic tissue to differentiate into IPCs. The in vitro differential function of these IPCs was compared by insulin-to-DNA ratios and C-peptide levels after glucose challenge. In vivo curative effects of IPCs transplanted into diabetic rats were monitored by weekly blood glucose measurement. Results. WJ-MSCs showed better proliferation and differentiation potential than pancreatic MSCs and BM-MSCs. In vivo, WJ-IPCs significantly reduced blood glucose levels at first week after transplantation and maintained significant decrease till week 8. BM-IPCs reduced blood glucose levels at first week but gradually increased since week 3. In resected pancreas-IPCs group, blood glucose levels were significantly reduced till two weeks after transplantation and gradually increased since week 4. Conclusion. WJ-MSCs are the most promising stem cell source for β-cell regeneration in diabetes treatment.
authors: Ten-Chan General Hospital Zhongli, Taoyuan City 112, Taiwan.
authors: Shih-Yi
authors: SY
authors: Kao
authors: Department of Biology and Anatomy, National Defense Medical Center, Taipei 112, Taiwan.
authors: Jia-Fwu
authors: JF
authors: Shyu
authors: Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
authors: Hwai-Shi
authors: HS
authors: Wang
authors: Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan ; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan.
authors: Chi-Hung
authors: CH
authors: Lin
authors: Department of Surgery, Cheng Hsin General Hospital, Taipei 112, Taiwan.
authors: Cheng-Hsi
authors: CH
authors: Su
authors: Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan ; Department of Surgery, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors: Tien-Hua
authors: TH
authors: Chen
authors: Division of Cardiovascular Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei 112, Taiwan ; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 112, Taiwan.
authors: Zen-Chung
authors: ZC
authors: Weng
authors: Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan ; Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan ; Department of Critical Care Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors: Pei-Jiun
authors: PJ
authors: Tsai