title: Protein-Containing Multilayer Capsules by Templating on Mesoporous CaCO3 Particles: POST- and PRE-Loading Approaches.
abstract: Encapsulation of model proteins (catalase, insulin, aprotinin) into multilayer dextran sulphate/protamin capsules by templating on CaCO3 microparticles is investigated employing: (i) PRE-loading into CaCO3 particles by adsorption or co-synthesis and (ii) POST-loading into performed capsules. Protein encapsulation is governed by both its size and electrostatic interactions with the carbonate microparticles and multilayer shell. PRE-loading enables improved encapsulation compared to POST-loading (catalase content in capsules 630 and 70 mg · g(-1)). Bioactivity of encapsulated protein is not affected by interaction with multilayers but may be reduced at slightly alkaline pH due to CaCO3 hydrolysis. This study might help to successfully encapsulate fragile bio-macromolecules into multilayer capsules.
authors: Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russia.
authors: Nadezhda G
authors: NG
authors: Balabushevich
authors: Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russia.
authors: Anna V
authors: AV
authors: Lopez de Guerenu
authors: Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russia.|Fraunhofer Institute for Cell Therapy and Immunology, Am Mühlenberg 13, 14476, Potsdam-Golm, Germany.
authors: Natalia A
authors: NA
authors: Feoktistova
authors: University of Ghent, Department of Molecular Biotechnology & NB-Photonics, Coupure Links 653, 9000, Gent, Belgium.
authors: Andre G
authors: AG
authors: Skirtach
authors: Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russia. Dmitry.Volodkin@izi-bb.fraunhofer.de.|Fraunhofer Institute for Cell Therapy and Immunology, Am Mühlenberg 13, 14476, Potsdam-Golm, Germany. Dmitry.Volodkin@izi-bb.fraunhofer.de.
authors: Dmitry
authors: D
authors: Volodkin
mesh_terms: D007611:Aprotinin / Q000008:administration & dosage; D002119:Calcium Carbonate; D002214:Capsules / Q000737:chemistry*; D002374:Catalase / Q000008:administration & dosage; D003911:Dextrans; D016503:Drug Delivery Systems; D007328:Insulin / Q000008:administration & dosage; D011479:Protamines
chemical_list: D002214:Capsules; D003911:Dextrans; D007328:Insulin; D011479:Protamines; D007611:Aprotinin; D002374:Catalase; D002119:Calcium Carbonate
keywords: CaCO microparticles; adsorption; co-synthesis; layer-by-layer; protein encapsulation