title: Transcriptome Analysis of the Preterm Rabbit Lung after Seven Days of Hyperoxic Exposure.
abstract: The neonatal management of preterm born infants often results in damage to the developing lung and subsequent morbidity, referred to as bronchopulmonary dysplasia (BPD). Animal models may help in understanding the molecular processes involved in this condition and define therapeutic targets. Our goal was to identify molecular pathways using the earlier described preterm rabbit model of hyperoxia induced lung-injury. Transcriptome analysis by mRNA-sequencing was performed on lungs from preterm rabbit pups born at day 28 of gestation (term: 31 days) and kept in hyperoxia (95% O2) for 7 days. Controls were preterm pups kept in normoxia. Transcriptomic data were analyzed using Array Studio and Ingenuity Pathway Analysis (IPA), in order to identify the central molecules responsible for the observed transcriptional changes. We detected 2217 significantly dysregulated transcripts following hyperoxia, of which 90% could be identified. Major pathophysiological dysregulations were found in inflammation, lung development, vascular development and reactive oxygen species (ROS) metabolism. To conclude, amongst the many dysregulated transcripts, major changes were found in the inflammatory, oxidative stress and lung developmental pathways. This information may be used for the generation of new treatment hypotheses for hyperoxia-induced lung injury and BPD.
authors: University Hospitals Leuven, Department of Pediatrics, Leuven, Belgium.
authors: Thomas
authors: T
authors: Salaets
authors: Department of Development and Regeneration, Research Unit Fetus Placenta Neonate, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium; University Hospitals Leuven, Department of Obstetrics and Gynecology, Leuven, Belgium.
authors: Jute
authors: J
authors: Richter
authors: Department of Genetics, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium.
authors: Paul
authors: P
authors: Brady
authors: Department of Development and Regeneration, Research Unit Fetus Placenta Neonate, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium; Clínica Alemana, Departamento Ginecología y Obstetricia, Santiago, Chile.
authors: Julio
authors: J
authors: Jimenez
authors: Department of Development and Regeneration, Research Unit Fetus Placenta Neonate, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium; Ehime Prefectural Central Hospital, Department of Neonatology, Matsuyama, Japan.
authors: Taro
authors: T
authors: Nagatomo
authors: Department of Development and Regeneration, Research Unit Fetus Placenta Neonate, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium; University Hospitals Leuven, Department of Obstetrics and Gynecology, Leuven, Belgium.
authors: Jan
authors: J
authors: Deprest
authors: University Hospitals Leuven, Department of Pediatrics, Leuven, Belgium; Department of Development and Regeneration, Research Unit Fetus Placenta Neonate, Group of Biomedical Sciences, KU Leuven, Leuven, Belgium.
authors: Jaan
authors: J
authors: Toelen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D053263:Gene Regulatory Networks; D018496:Hyperoxia / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000254:growth & development* / Q000378:metabolism; D055370:Lung Injury / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D011817:Rabbits; D059467:Transcriptome