title: [Characteristics of refractive status of mutant Lumican transgenic mice].
abstract: OBJECTIVE
  To investigate the change of refractive status in transgenic mice with mutant Lumican (bright proteoglycan) gene at different ages.
  
  
  METHODS
  Experimental Study. Fifty-four 3-week-old with mutant Lumican gene (cDNA 596T > C) mice (27 male and 27 female) were randomly divided into 9 groups (n = 6, half male and half female) by random number table. One group (3-week-old) was randomly chosen and measured the refractive status by retinoscopy after mydriasis. Measurement of other groups were repeated the method above respectively in the fourth, fifth, sixth, eighth, tenth, twelfth, sixteenth, and twentieth week. Differences of diopter between right and left eye and between male and female were compared within each group by paired t test. The differences of mice's diopters in different age were compared by Kruskal-Wallis H test. Pairwise comparisons were acquired by Mann-Whitney U test.
  
  
  RESULTS
  There were no statistic difference of diopters between binoculus: The mice's diopters of right and left eyes were respective measured in the twentieth week (1.50 ± 0.45) D and (1.25 ± 0.42) D (t = -0.889, P > 0.05), The mice's diopters of right and left eyes were respective measured in the third week (-2.50 ± 2.59) D and (-2.50 ± 4.32) D (t = 0.000, P > 0.05); There were no statistic difference of diopters between different genders: The mice's diopters of female and male were respective measured in the third week (-0.5 ± 3.83) D and (-4.17 ± 1.94) D, (t = 2.079, P > 0.05), The mice's diopters of female and male were respective measured in the twelfth week (1.50 ± 0.84) D and (1.50 ± 1.87) D (t = 0.000, P > 0.05); Analysis of binocular diopters revealed significant differences among nine groups (H = 20.910, P < 0.05). Diopters measured in the third week (-2.50 ± 3.40D) and the sixth week (+3.25 ± 2.67) D had statistical difference (Z = -3.259, P < 0.001). There were no statistical significance between other groups (P > 0.001).
  
  
  CONCLUSIONS
  Characteristics of diopters gathered from mice with Lumican gene mutation at different weeks are summarized as follows: Myopia could be observed in the third week. And this situation of myopia was gradually transformed into hyperopia with aging. The maximum hyperopic diopter was observed at 6th-week-old mice. From the eighth to twentieth week, the degree of hyperopic diopter gradually decreased and stabilized.
authors: Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University, Beijing Key Lab. of Ophthalmology & Visual Sciences, Beijing 100730, China.
authors: Jun
authors: J
authors: Tao
authors: Yanzheng
authors: Y
authors: Song
authors: Email: wxw93@hotmail.com.
authors: Fengju
authors: F
authors: Zhang
authors: Mingsheng
authors: M
authors: Sun
authors: Yuanbin
authors: Y
authors: Liu
mesh_terms: D000367:Age Factors; D000818:Animals; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D006956:Hyperopia / Q000235:genetics* / Q000503:physiopathology; D007632:Keratan Sulfate / Q000235:genetics*; D000071234:Lumican; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009216:Myopia / Q000235:genetics* / Q000503:physiopathology; D011897:Random Allocation; D012029:Refraction, Ocular / Q000235:genetics*; D012737:Sex Factors
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C000604307:Lum protein, mouse; D000071234:Lumican; D007632:Keratan Sulfate