title: CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1.
abstract: The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors: Zhengfu
authors: Z
authors: Zhou
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors: Wei
authors: W
authors: Zhang
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors: Shiyou
authors: S
authors: Su
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors: Ming
authors: M
authors: Chen
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors: Wei
authors: W
authors: Lu
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors: Min
authors: M
authors: Lin
authors: Natural Products Center, School of Natural Resources and the Environment, University of Arizona, Tucson, AZ, 85706, USA. imolnar@email.arizona.edu.
authors: István
authors: I
authors: Molnár
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. xuyuquan@caas.cn.
authors: Yuquan
authors: Y
authors: Xu
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D034301:Deinococcus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D055786:Gene Knockout Techniques; D050296:Microbial Viability; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D016877:Oxidants; D018384:Oxidative Stress; D014466:Ultraviolet Rays
chemical_list: D016877:Oxidants; C547822:deinoxanthin; D002338:Carotenoids; D006899:Mixed Function Oxygenases
keywords: 2-β-hydroxylase; CYP287A1; Cytochrome P450; Deinococcus radiodurans; Deinoxanthin