title: The effect of resuscitation in 100% oxygen on brain injury in a newborn rat model of severe hypoxic-ischaemic encephalopathy.
abstract: OBJECTIVE
  Infants with birth asphyxia frequently require resuscitation. Current guidance is to start newborn resuscitation in 21% oxygen. However, infants with severe hypoxia-ischaemia may require prolonged resuscitation with oxygen. To date, no study has looked at the effect of resuscitation in 100% oxygen following a severe hypoxic-ischaemic insult.
  
  
  METHODS
  Postnatal day 7 Wistar rats underwent a severe hypoxic-ischaemic insult (modified Vannucci unilateral brain injury model) followed by immediate resuscitation in either 21% or 100% oxygen for 30 min. Seven days following the insult, negative geotaxis testing was performed in survivors, and the brains were harvested. Relative ipsilateral cortical and hippocampal area loss was assessed histologically.
  
  
  RESULTS
  Total area loss in the affected hemisphere and area loss within the hippocampus did not significantly differ between the two groups. The same results were seen for short-term neurological assessment. No difference was seen in weight gain between pups resuscitated in 21% and 100% oxygen.
  
  
  CONCLUSIONS
  Resuscitation in 100% oxygen does not cause a deleterious effect on brain injury following a severe hypoxic-ischaemic insult in a rat model of hypoxia-ischaemia. Further work investigating the effects of resuscitation in 100% oxygen is warranted, especially for newborn infants with severe hypoxic-ischaemic encephalopathy.
authors: Neonatal Neuroscience, School of Clinical Sciences, University of Bristol, Bristol, United Kingdom.
authors: Elisa
authors: E
authors: Smit
authors: Neonatal Neuroscience, School of Clinical Sciences, University of Bristol, Bristol, United Kingdom.
authors: Xun
authors: X
authors: Liu
authors: Neonatal Neuroscience, School of Clinical Sciences, University of Bristol, Bristol, United Kingdom.
authors: Hannah
authors: H
authors: Gill
authors: Neonatal Neuroscience, School of Clinical Sciences, University of Bristol, Bristol, United Kingdom.
authors: Sally
authors: S
authors: Jary
authors: Division of Physiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
authors: Thomas
authors: T
authors: Wood
authors: Neonatal Neuroscience, School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Division of Physiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. Electronic address: Marianne.thoresen@bristol.ac.uk.
authors: Marianne
authors: M
authors: Thoresen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002545:Brain Ischemia / Q000175:diagnosis / Q000209:etiology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D002534:Hypoxia, Brain; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D010102:Oxygen Inhalation Therapy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation / Q000379:methods*; D012720:Severity of Illness Index
chemical_list: D010100:Oxygen
keywords: Asphyxia; Hyperoxia; Newborn resuscitation; Oxidative stress; Rat model