title: Imaging Fibrosis and Separating Collagens using Second Harmonic Generation and Phasor Approach to Fluorescence Lifetime Imaging.
abstract: In this paper we have used second harmonic generation (SHG) and phasor approach to auto fluorescence lifetime imaging (FLIM) to obtain fingerprints of different collagens and then used these fingerprints to observe bone marrow fibrosis in the mouse femur. This is a label free approach towards fast automatable detection of fibrosis in tissue samples. FLIM has previously been used as a method of contrast in different tissues and in this paper phasor approach to FLIM is used to separate collagen I from collagen III, the markers of fibrosis, the largest groups of disorders that are often without any effective therapy. Often characterized by an increase in collagen content of the corresponding tissue, the samples are usually visualized by histochemical staining, which is pathologist dependent and cannot be automated.
authors: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, California.
authors: Suman
authors: S
authors: Ranjit
authors: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, California.
authors: Alexander
authors: A
authors: Dvornikov
authors: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, California.
authors: Milka
authors: M
authors: Stakic
authors: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA.
authors: Suk-Hyun
authors: SH
authors: Hong
authors: Division of Renal Diseases and Hypertension, University of Colorado at Denver, Aurora, Colorado.
authors: Moshe
authors: M
authors: Levi
authors: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA.
authors: Ronald M
authors: RM
authors: Evans
authors: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, California.
authors: Enrico
authors: E
authors: Gratton
mesh_terms: D000818:Animals; D002645:Chickens; D003094:Collagen / Q000302:isolation & purification*; D005269:Femur / Q000473:pathology; D005355:Fibrosis / Q000175:diagnosis*; D005782:Gels; D006801:Humans; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D051381:Rats; D012815:Signal Processing, Computer-Assisted
chemical_list: D005782:Gels; D003094:Collagen