title: Evaluation of rMETase-Loaded Stealth PLGA/Liposomes Modified with Anti-CAGE scFV for Treatment of Gastric Carcinoma.
abstract: Stealth PLGA/Liposome nanoparticles (NPs) modified with tumor-targeting single-chain antibody fragment (scFV-P/L) for systemic delivery of recombinant methioninase (rMETase) for gastric cancer were prepared. The morphologies and therapeutic effects of rMETase-loaded scFV-P/L (scFV-rMETase-P/L) in vitro were analyzed. Functional scFV-P/L NPs composed of PLGA, DOPC and DSPE-PEG display low cell cytoxicity in SGC-7901 cells, and has more cell uptake ability than P/L NPs. scFV-rMETase-P/L was more effective in inhibiting tumor growth in the subcutaneous gastric carcinoma tumor model than free rMETase in solution (p < 0.05) and rMETase-loaded P/L (rMETase-P/L) (p < 0.05). Our findings collectively support the utility of scFV-targeted P/L NPs as a potentially effective drug delivery system.
authors: Lin
authors: L
authors: Xin
authors: Jia-Qing
authors: JQ
authors: Caot
authors: Chuan
authors: C
authors: Liu
authors: Fei
authors: F
authors: Zeng
authors: Hua
authors: H
authors: Cheng
authors: Xiao-Yun
authors: XY
authors: Hu
authors: Jiang-Hua
authors: JH
authors: Shao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D013437:Carbon-Sulfur Lyases / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053487:DEAD-box RNA Helicases / Q000276:immunology; D004058:Diffusion; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D057127:Single-Chain Antibodies / Q000276:immunology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D008081:Liposomes; D053769:Nanocapsules; D058726:Nanoconjugates; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C454199:DDX53 protein, human; D053487:DEAD-box RNA Helicases; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase