title: The Peanut (Arachis hypogaea L.) Gene AhLPAT2 Increases the Lipid Content of Transgenic Arabidopsis Seeds.
abstract: Lysophosphatidic acid acyltransferase (LPAT), which converts lysophosphatidic acid (LPA) to phosphatidic acid (PA), catalyzes the addition of fatty acyl moieties to the sn-2 position of the LPA glycerol backbone in triacylglycerol (TAG) biosynthesis. We recently reported the cloning and temporal-spatial expression of a peanut (Arachis hypogaea) AhLPAT2gene, showing that an increase in AhLPAT2 transcript levels was closely correlated with an increase in seed oil levels. However, the function of the enzyme encoded by the AhLPAT2 gene remains unclear. Here, we report that AhLPAT2 transcript levels were consistently higher in the seeds of a high-oil cultivar than in those of a low-oil cultivar across different seed developmental stages. Seed-specific overexpression of AhLPAT2 in Arabidopsis results in a higher percentage of oil in the seeds and greater-than-average seed weight in the transgenic plants compared with the wild-type plants, leading to a significant increase in total oil yield per plant. The total fatty acid (FA) content and the proportion of unsaturated FAs also increased. In the developing siliques of AhLPAT2-overexpressing plants, the expression levels of genes encoding crucial enzymes involved in de novo FA synthesis, acetyl-CoA subunit (AtBCCP2) and acyl carrier protein 1 (AtACP1) were elevated. AhLPAT2 overexpression also promoted the expression of several key genes related to TAG assembly, sucrose metabolism, and glycolysis. These results demonstrate that the expression of AhLPAT2 plays an important role in glycerolipid production in peanuts.
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Silong
authors: S
authors: Chen
authors: Key Laboratory of Biology and the Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the ChineseAcademy of Agricultural Sciences, Wuhan, China.
authors: Yong
authors: Y
authors: Lei
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Xian
authors: X
authors: Xu
authors: Key Laboratory of Biology and the Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the ChineseAcademy of Agricultural Sciences, Wuhan, China.
authors: Jiaquan
authors: J
authors: Huang
authors: Key Laboratory of Biology and the Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the ChineseAcademy of Agricultural Sciences, Wuhan, China.
authors: Huifang
authors: H
authors: Jiang
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Jin
authors: J
authors: Wang
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Zengshu
authors: Z
authors: Cheng
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Jianan
authors: J
authors: Zhang
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Yahui
authors: Y
authors: Song
authors: Key Laboratory of Biology and the Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the ChineseAcademy of Agricultural Sciences, Wuhan, China.
authors: Boshou
authors: B
authors: Liao
authors: Hebei Provincial Laboratory of Crop Genetics and Breeding, Cereal and Oil Crop Institute, HebeiAcademy of Agricultural and Forestry Science, Shijiazhuang, China.
authors: Yurong
authors: Y
authors: Li
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010367:Arachis / Q000235:genetics*; D005227:Fatty Acids / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008055:Lipids / Q000737:chemistry* / Q000235:genetics; D010938:Plant Oils / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D014280:Triglycerides / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010938:Plant Oils; D014280:Triglycerides; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase