title: Construction of a highly efficient Bacillus subtilis 168 whole-cell biocatalyst and its application in the production of L-ornithine.
abstract: L-Ornithine, a non-protein amino acid, is usually extracted from hydrolyzed protein as well as produced by microbial fermentation. Here, we focus on a highly efficient whole-cell biocatalyst for the production of L-ornithine. The gene argI, encoding arginase, which catalyzes the hydrolysis of L-arginine to L-ornithine and urea, was cloned from Bacillus amyloliquefaciens B10-127 and expressed in GRAS strain Bacillus subtilis 168. The recombinant strain exhibited an arginase activity of 21.9 U/mg, which is 26.7 times that of wild B. subtilis 168. The optimal pH and temperature of the purified recombinant arginase were 10.0 and 40 °C, respectively. In addition, the recombinant arginase exhibited a strong Mn(2+) preference. When using whole-cell biocatalyst-based bioconversion, a hyper L-ornithine production of 356.9 g/L was achieved with a fed-batch strategy in a 5-L reactor within 12 h. This whole-cell bioconversion study demonstrates an environmentally friendly strategy for L-ornithine production in industry.
authors: The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
authors: Meizhou
authors: M
authors: Wang
authors: The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
authors: Meijuan
authors: M
authors: Xu
authors: The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China. raozhm@jiangnan.edu.cn.
authors: Zhiming
authors: Z
authors: Rao
authors: The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
authors: Taowei
authors: T
authors: Yang
authors: The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
authors: Xian
authors: X
authors: Zhang
mesh_terms: D001119:Arginase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001412:Bacillus subtilis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D055162:Biocatalysis; D019149:Bioreactors; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000378:metabolism; D009952:Ornithine / Q000096:biosynthesis*; D013696:Temperature; D013997:Time Factors; D014508:Urea / Q000378:metabolism
chemical_list: D008345:Manganese; D014508:Urea; D001120:Arginine; D009952:Ornithine; D001119:Arginase
keywords: B. subtilis 168; L-Ornithine; Recombinant arginase; Whole-cell bioconversion