title: [Biochip detection of KRAS, BRAF, and PIK3CA somatic mutations in colorectal cancer patients].
abstract: Somatic mutations of KRAS, PIK3CA, and BRAF cause insensitivity of colorectal tumors to therapy with anti-EGFR monoclonal antibodies, necessitating a genetic testing prior to therapy. A biological microchip was developed and validated to allow detection of 19 somatic mutations in KRAS, PIK3CA, and BRAF genes. The method combines LNA-clamp PCR and allele-specific hybridization on a microchip and detects mutant DNA in 100 times wild-type background (1%). A total of 66 DNA samples isolated from colorectal tumors were tested with the biochip. Possible associations between the genetic status of the tumor and the patient's characteristics (age, sex, tumor localization, stage, and TMN) were assessed statistically. KRAS mutations were more common in females (P = 0.02) and in patients with distant metastasis (P = 0.04). Other associations between the presence of mutations and patient characteristics were not observed. The method proved highly sensitive and can be used in oncology to select patients who sensitive to therapy with anti-EGFR monoclonal antibodies.
authors: BIOCHIP-IMB Ltd., Moscow, 117312 Russia.|Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.|emel_marina@mail.ru.
authors: M A
authors: MA
authors: Emelyanova
authors: Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.|Medical Genetic Research Center, Moscow, 115478 Russia.
authors: F A
authors: FA
authors: Amossenko
authors: Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
authors: A V
authors: AV
authors: Semyanikhina
authors: Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
authors: V A
authors: VA
authors: Aliev
authors: Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
authors: Yu A
authors: YA
authors: Barsukov
authors: Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
authors: L N
authors: LN
authors: Lyubchenko
authors: BIOCHIP-IMB Ltd., Moscow, 117312 Russia.|Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors: T V
authors: TV
authors: Nasedkina
keywords: BRAF; KRAS; LNA-clamp PCR; PIK3CA; biochips; colorectal cancer; somatic mutations