title: In Vitro Analyses of T Cell Effector Differentiation.
abstract: In vitro culture is an important complement, or substitute, to in vivo approaches in order to study T cell effector differentiation. Here, we describe culture conditions that generate specific effector cell types by exposing naïve T cells to appropriate cytokine signals.
authors: Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), 251 Biomedical Research Building, Buffalo, NY, 14214, USA, wohlfert@buffalo.edu.
authors: Elizabeth A
authors: EA
authors: Wohlfert
authors: Andrea C
authors: AC
authors: Carpenter
authors: Yasmine
authors: Y
authors: Belkaid
authors: Rémy
authors: R
authors: Bosselut
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016130:Immunophenotyping; D066298:In Vitro Techniques; D051379:Mice; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism*