title: Impact of Melatonin Enrichment during Germination of Legumes on Bioactive Compounds and Antioxidant Activity.
abstract: This study assesses the impact of melatonin enriched watering on the germination of lentils (Lens culinaris L.) and kidney beans (Phaseolus vulgaris L.). The melatonin levels in lentil and bean sprouts measured by HPLC-MS/MS were more important than those found in other legumes and sprouts, being higher in lentil (1090 ng/g) than in kidney bean (529 ng/g) sprouts. This alternative germination promoted a significant increase of the development of radicles in comparison with the traditional germination. The decreases in the phenolic load were less accentuated than previously observed (lentil sprouts displayed 394 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)), probably due to the protective effect of melatonin. The antioxidant capacity (oxygen radical absorbing capacity assay) increased in these sprouts, reaching 85 and 56 μmol of Trolox equivalents/g DW in lentils and beans, respectively. Hence, the melatonin-enriched foods exhibited potent free radical scavenger and antioxidant functions that may be used as a nutritional strategy to alleviate and prevent chronic and age-related diseases.
authors: Instituto de Investigación de Ciencias de la Alimentación (CIAL), Facultad de Ciencias, Universidad Autónoma de Madrid , C/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors: Yolanda
authors: Y
authors: Aguilera
authors: Instituto de Investigación de Ciencias de la Alimentación (CIAL), Facultad de Ciencias, Universidad Autónoma de Madrid , C/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors: Teresa
authors: T
authors: Herrera
authors: Instituto de Investigación de Ciencias de la Alimentación (CIAL), Facultad de Ciencias, Universidad Autónoma de Madrid , C/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors: Rosa
authors: R
authors: Liébana
authors: Instituto de Investigación de Ciencias de la Alimentación (CIAL), Facultad de Ciencias, Universidad Autónoma de Madrid , C/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors: Miguel
authors: M
authors: Rebollo-Hernanz
authors: Instituto de Investigación de Ciencias de la Alimentación (CIAL), Facultad de Ciencias, Universidad Autónoma de Madrid , C/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors: Carlos
authors: C
authors: Sanchez-Puelles
authors: Instituto de Investigación de Ciencias de la Alimentación (CIAL), Facultad de Ciencias, Universidad Autónoma de Madrid , C/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors: María A
authors: MA
authors: Martín-Cabrejas
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D018525:Germination; D027806:Lens Plant / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D008550:Melatonin / Q000032:analysis* / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010636:Phenols; D008550:Melatonin
keywords: antioxidant capacity; exogenous melatonin; germination; legumes; melatonin; phenolic compounds