title: Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy.
abstract: In this work a method for measuring brain oxygen partial pressure with confocal phosphorescence lifetime microscopy system is reported. When used in conjunction with a dendritic phosphorescent probe, Oxyphor G4, this system enabled minimally invasive measurements of oxygen partial pressure (pO2) in cerebral tissue with high spatial and temporal resolution during 4-AP induced epileptic seizures. Investigating epileptic events, we characterized the spatio-temporal distribution of the "initial dip" in pO2 near the probe injection site and along nearby arterioles. Our results reveal a correlation between the percent change in the pO2 signal during the "initial dip" and the duration of seizure-like activity, which can help localize the epileptic focus and predict the length of seizure.
authors: École Polytechnique de Montréal, Department of Electrical Engineering, C.P. 6079 succ.Centre-ville, Montreal, Quebec, Canada, H3C 3A7.
authors: Cong
authors: C
authors: Zhang
authors: École Polytechnique de Montréal, Department of Electrical Engineering, C.P. 6079 succ.Centre-ville, Montreal, Quebec, Canada, H3C 3A7.
authors: Samuel
authors: S
authors: Bélanger
authors: École Polytechnique de Montréal, Department of Electrical Engineering, C.P. 6079 succ.Centre-ville, Montreal, Quebec, Canada, H3C 3A7; Montreal Heart Institute, 5000 Bélanger Est, Montreal, Quebec, Canada, H1T 1C8.
authors: Philippe
authors: P
authors: Pouliot
authors: École Polytechnique de Montréal, Department of Electrical Engineering, C.P. 6079 succ.Centre-ville, Montreal, Quebec, Canada, H3C 3A7; Montreal Heart Institute, 5000 Bélanger Est, Montreal, Quebec, Canada, H1T 1C8.
authors: Frédéric
authors: F
authors: Lesage
mesh_terms: D015761:4-Aminopyridine; D000792:Angiography; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004827:Epilepsy / Q000175:diagnosis* / Q000503:physiopathology; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008810:Mice, Inbred C57BL; D008853:Microscopy / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D012640:Seizures / Q000503:physiopathology; D013696:Temperature
chemical_list: D015761:4-Aminopyridine; D010100:Oxygen