title: Biodiesel production from non-edible lignocellulosic biomass of Cassia fistula L. fruit pulp using oleaginous yeast Rhodosporidium kratochvilovae HIMPA1.
abstract: This study explored biodiesel production from a low cost, abundant, non-edible lignocellulosic biomass from aqueous extract of Cassia fistula L. (CAE) fruit pulp. The CAE was utilized as substrate for cultivating novel oleaginous yeast Rhodosporidium kratochvilovae HIMPA1. This oleaginous yeast accumulates high amount of triacylglycerides as large intracellular lipid droplets (4.35±0.54μm) using CAE as sole nutritional source. Total lipids (4.86±0.54g/l) with lipid content of 53.18% (w/w) were produced by R. kratochvilovae HIMPA1 on CAE. The FAME profile obtained revealed palmitic acid (C16:0) 43.06%, stearic acid (C18:0) 28.74%, and oleic acid (C18:1) 17.34% as major fatty acids. High saturated fatty acids content (72.58%) can be blended with high PUFA feedstocks to make it an industrially viable renewable energy product.
authors: Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors: Alok
authors: A
authors: Patel
authors: Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors: Dev K
authors: DK
authors: Sindhu
authors: Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors: Neha
authors: N
authors: Arora
authors: Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors: Rajesh P
authors: RP
authors: Singh
authors: Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors: Vikas
authors: V
authors: Pruthi
authors: Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India. Electronic address: parulaggarwalpruthi@gmail.com.
authors: Parul A
authors: PA
authors: Pruthi
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D056804:Biofuels; D018533:Biomass; D002366:Cassia / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005227:Fatty Acids
keywords: Biodiesel; Cassia fistula; Lignocellulosic biomass; Oleaginous yeast; Rhodosporidium