title: Exometabolom analysis of breast cancer cell lines: Metabolic signature.
abstract: Cancer cells show characteristic effects on cellular turnover and DNA/RNA modifications leading to elevated levels of excreted modified nucleosides. We investigated the molecular signature of different subtypes of breast cancer cell lines and the breast epithelial cell line MCF-10A. Prepurification of cell culture supernatants was performed by cis-diol specific affinity chromatography using boronate-derivatized polyacrylamide gel. Samples were analyzed by application of reversed phase chromatography coupled to a triple quadrupole mass spectrometer. Collectively, we determined 23 compounds from RNA metabolism, two from purine metabolism, five from polyamine/methionine cycle, one from histidine metabolism and two from nicotinate and nicotinamide metabolism. We observed major differences of metabolite excretion pattern between the breast cancer cell lines and MCF-10A, just as well as between the different breast cancer cell lines themselves. Differences in metabolite excretion resulting from cancerous metabolism can be integrated into altered processes on the cellular level. Modified nucleosides have great potential as biomarkers in due consideration of the heterogeneity of breast cancer that is reflected by the different molecular subtypes of breast cancer. Our data suggests that the metabolic signature of breast cancer cell lines might be a more subtype-specific tool to predict breast cancer, rather than a universal approach.
authors: Center for Biological Systems Analysis ZBSA, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.|Institute of Biology II, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.
authors: Lucas
authors: L
authors: Willmann
authors: University Medical Center Freiburg, 79106 Freiburg, Germany.
authors: Thalia
authors: T
authors: Erbes
authors: Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.|Institute for Biology III, Faculty of Biology, Albert-Ludwigs-University Freiburg, Germany.|Spemann Graduate School of Biology and Medicine, Albert-Ludwigs-University Freiburg, Germany.
authors: Sebastian
authors: S
authors: Halbach
authors: Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.
authors: Tilman
authors: T
authors: Brummer
authors: University Medical Center Freiburg, 79106 Freiburg, Germany.
authors: Markus
authors: M
authors: Jäger
authors: Department of Obstetrics and Gynecology, University Hospital Freiburg, Freiburg 79106, Germany.|German Cancer Consortium (DKTK), Heidelberg, Germany.|German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors: Marc
authors: M
authors: Hirschfeld
authors: Universitätsfrauenklinik der Heinrich-Heine-Universität Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.
authors: Tanja
authors: T
authors: Fehm
authors: Universitätsfrauenklinik der Heinrich-Heine-Universität Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.
authors: Hans
authors: H
authors: Neubauer
authors: University Medical Center Freiburg, 79106 Freiburg, Germany.
authors: Elmar
authors: E
authors: Stickeler
authors: Center for Biological Systems Analysis ZBSA, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.
authors: Bernd
authors: B
authors: Kammerer
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D012334:RNA, Neoplasm / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D012334:RNA, Neoplasm