title: Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage.
abstract: The objective of this study was to describe a new method for the quantitative analysis of a microleakage of endodontic filling materials. Forty extracted single-rooted teeth were randomly divided into three experimental groups. After root canal shaping, the experimental groups were filled using the lateral condensation technique with the Epiphany system (G1), with gutta-percha + Sealapex (G2), and with gutta-percha + AH Plus (G3). Each root was mounted on a modified leakage testing device, and caffeine solution was used as a tracer (2000 ng mL-1, pH 6.0), applied in the coronal direction towards the tooth apex, creating a hydrostatic pressure of 2.55 kPa. Presence of caffeine in the receiving solution was measured after 10, 30, and 60 days, using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). None of the groups presented microleakage at 10 days. At 30 days, G2 and G3 showed similar infiltration patterns (means: 16.0 and 13.9 ng mL-1, respectively), whereas G1 showed significantly higher values (mean: 105.2 ng mL-1). At 60 days, leakage values were 182.6 ng mL-1 for G1, 139.0 ng mL-1 for G2, and 53.5 ng mL-1 for G3. AH Plus showed the best sealing ability and HPLC-MS/MS showed high sensitivity and specificity for tracer quantification.
authors: Department of Endodontics, School of Dentistry, Universidade Tuiuti do Paraná, Curitiba, PR, BR.
authors: André Luiz da Costa
authors: AL
authors: Michelotto
authors: Department of Pharmacy, Health Sciences, Universidade Federal do Paraná, Curitiba, PR, BR.
authors: João Cleverson
authors: JC
authors: Gasparetto
authors: Department of Pharmacy, Health Sciences, Universidade Federal do Paraná, Curitiba, PR, BR.
authors: Francinete Ramos
authors: FR
authors: Campos
authors: Department of Endodontics, School of Dentistry, Universidade Federal do Paraná, Curitiba, PR, BR.
authors: Gilson Blitzkow
authors: GB
authors: Sydney
authors: Department of Pharmacy, Health Sciences, Universidade Federal do Paraná, Curitiba, PR, BR.
authors: Roberto
authors: R
authors: Pontarolo
mesh_terms: D002126:Calcium Hydroxide; D002853:Chromatography, Liquid; D003763:Dental Leakage / Q000175:diagnosis* / Q000517:prevention & control; D004853:Epoxy Resins; D006171:Gutta-Percha; D006801:Humans; D011897:Random Allocation; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation / Q000295:instrumentation / Q000379:methods*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods*; D012459:Salicylates; D053719:Tandem Mass Spectrometry
chemical_list: D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; C055601:Sealapex; D006171:Gutta-Percha; D002126:Calcium Hydroxide