title: Retroviral Transduction of T Cells and T Cell Precursors.
abstract: Transduction of lymphoid progenitors with retroviral or lentiviral vectors is a powerful experimental strategy to tease out the role of a gene or pathway in T cell development via gain-of-function or loss-of-function strategies. Here we discuss different approaches to use this powerful technology, and present some protocols that we use to transduce murine HSCs, thymocytes, and lymphoid cell lines with these viral vectors.
authors: Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, MS 29, Oklahoma City, OK, 73104, USA.
authors: Amie
authors: A
authors: Simmons
authors: José
authors: J
authors: Alberola-Ila
mesh_terms: D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D051379:Mice; D054504:Precursor Cells, T-Lymphoid / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism*; D014161:Transduction, Genetic