title: [Effect of As₂O₃on Hedgehog Pathway in Chronic Myeloid Leukemia Cells].
abstract: OBJECTIVE
  To explore the effect of As₂O₃on Hedgehog pathway in chronic myeloid leukemia (CML) cells.
  
  
  METHODS
  The apoptosis of K562 cells was detected by MTT method and flow cytometry; the expressions of PTCH and SMO protein and mRNA in Hedgehog pathway were determined by Western blot and real-time PCR, respectively. Retults: The As₂O₃ could induce the apoptosis of K562 cells with optimal concentration 2 µmol/L and optimal time 24 hours. The expressions of PTCH and SMO protein and mRNA in Hedgehog pathway of K562 cells treated with As₂O₃at optimal concentration and optimal time were down-regulated.
  
  
  CONCLUSIONS
  The As₂O₃can down-regulate the expression of PTCH and SMO in Hedgehog pathway.
authors: Department of Hematology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, Liaoning Province, China.
authors: Xiao-Wei
authors: XW
authors: He
authors: Department of Hematology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, Liaoning Province, China.
authors: Wei
authors: W
authors: Yan
authors: Department of Hematology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, Liaoning Province, China. E-mail: yangw@sj-hospital.org.
authors: Wei
authors: W
authors: Yang
mesh_terms: D017209:Apoptosis; D000077237:Arsenic Trioxide; D001152:Arsenicals; D015536:Down-Regulation; D053823:Hedgehog Proteins; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D010087:Oxides; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D001152:Arsenicals; D053823:Hedgehog Proteins; D010087:Oxides; D012333:RNA, Messenger; D000077237:Arsenic Trioxide