title: High-performance thin-layer chromatography for the evaluation of voacamine intracellular concentration related to its cytotoxic effect.
abstract: Previous investigations demonstrated that pretreatment with non-cytotoxic concentrations of voacamine had a chemosensitizing effect on cultured multidrug resistant osteosarcoma cells exposed to doxorubicin; whereas when used alone at high concentrations voacamine induced apoptosis-independent cell death on both sensitive and resistant cells. To gain insight into the mechanism of action of voacamine at the subcellular level, we developed an analytical high-performance thin-layer chromatography technique to assess the intracellular content of voacamine that could be correlated with the induction of cell death and consequent morphological and ultrastructural changes. The results of the quantitative analysis not only did allow us to measure both the amount of unmodified voacamine molecules (determined by the method) and the amount of molecules which reacted with cellular components (undetectable), but also to confirm the findings of our previous studies and support the validity of this method.
authors: Department of Technology and Health, Italian National Institute of Health, 00161 Rome, Italy; Institute of Chemical Methodologies, National Research Council (CNR), 00185 Rome, Italy.
authors: Maria
authors: M
authors: Condello
authors: Department of Therapeutic Research and Medicines Evaluation, Italian National Institute of Health, 00161 Rome, Italy.
authors: Giuseppina
authors: G
authors: Multari
authors: Department of Therapeutic Research and Medicines Evaluation, Italian National Institute of Health, 00161 Rome, Italy.
authors: Francesca Romana
authors: FR
authors: Gallo
authors: Department of Technology and Health, Italian National Institute of Health, 00161 Rome, Italy.
authors: Giuseppe
authors: G
authors: Arancia
authors: Department of Technology and Health, Italian National Institute of Health, 00161 Rome, Italy. Electronic address: stefania.meschini@iss.it.
authors: Stefania
authors: S
authors: Meschini
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002855:Chromatography, Thin Layer / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D006801:Humans; D007050:Ibogaine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism* / Q000648:ultrastructure; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; C005891:voacamine; D007050:Ibogaine
keywords: Cytotoxicity; HPTLC-densitometry; High-performance thin-layer chromatography; Multidrug resistant tumor cells; Voacamine