title: Quantitation of nuclear factor kappa B activation in pancreatic acinar cells during rat acute pancreatitis by flow cytometry.
abstract: This study aimed to develop a specific and sensitive method for the rapid detection of NF-κB activity in pancreatic tissue. Male Wistar rats were randomly divided into two groups: (1) 16 rats in the acute pancreatitis (AP) group received retrograde injection of 5% sodium taurocholate (STC) into the biliopancreatic duct, and (2) 16 rats in the Control group received saline. NF-κB activation in rat pancreatic acinar cells was assessed by flow cytometry (FCM). We found that the NF-κB activity in the AP group significantly increased at 1.5 h (29.80%±7.83), had a peak at 3 h (65.17%±13.22), and then decreased gradually to 12 h time point, close to the level after 1.5 h stimulation of STC. The NF-κB activity of the Control group did not significantly vary at different time points (P>0.05). FCM is a specific and sensitive assay for the rapid detection of NF-κB activity in pancreatic tissue.
authors: Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Wenliang
authors: W
authors: Chen
authors: Department of Hematology, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Zhuanzhen
authors: Z
authors: Zheng
authors: Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Junfang
authors: J
authors: Duan
authors: Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Xiaoru
authors: X
authors: Wang
authors: Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Shirong
authors: S
authors: Wu
authors: Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Wei
authors: W
authors: Wang
authors: Department of Minimally Invasive Surgery of The 1st Affiliated Hospital of Soochow University Suzhou 215000, China.
authors: Lu
authors: L
authors: Xu
authors: Department of General Surgery and Surgical Intensive Care Units, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Shuguang
authors: S
authors: Han
authors: Department of Hematology, The 2nd Affiliated Hospital of Shanxi Medical University Taiyuan 030001, China.
authors: Zhenhua
authors: Z
authors: Qiao
keywords: Acute pancreatitis; flow cytometry; nuclear factor kappa B