title: Organotypic Spinal Cord Culture: a Proper Platform for the Functional Screening.
abstract: Recent improvements in organotypic slice culturing and its accompanying technological innovations have made this biological preparation increasingly useful ex vivo experimental model. Among organotypic slice cultures obtained from various central nervous regions, spinal cord slice culture is an absorbing model that represents several unique advantages over other current in vitro and in vivo models. The culture of developing spinal cord slices, as allows real-time observation of embryonic cells behaviors, is an instrumental platform for developmental investigation. Importantly, due to the ability of ex vivo models to recapitulate different aspects of corresponding in vivo conditions, these models have been subject of various manipulations to derive disease-relevant slice models. Moreover spinal cord slice cultures represent a potential platform for screening of different pharmacological agents and evaluation of cell transplantation and neuroregenerative materials. In this review, we will focus on studies carried out using the ex vivo model of spinal cord slice cultures and main advantages linked to practicality of these slices in both normal and neuropathological diseases and summarize them in different categories based on application.
authors: Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
authors: Sareh
authors: S
authors: Pandamooz
authors: Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
authors: Mohammad
authors: M
authors: Nabiuni
authors: Neurobiology Research Group, Faculty of Life Sciences, The University of Manchester, Manchester, UK.
authors: Jaleel
authors: J
authors: Miyan
authors: Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Abolhassan
authors: A
authors: Ahmadiani
authors: NeuroBiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. l.dargahi@sbmu.ac.ir.
authors: Leila
authors: L
authors: Dargahi
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000639:trends; D006801:Humans; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D009422:Nervous System Diseases / Q000188:drug therapy / Q000473:pathology*; D009924:Organ Culture Techniques; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
chemical_list: D020932:Nerve Growth Factor
keywords: Ex vivo; Experimental model; Organotypic culture; Spinal cord