title: Antigenotoxic and Antimutagenic Activities of Probiotic Lactobacillus rhamnosus Vc against N-Methyl-N'-Nitro-N-Nitrosoguanidine.
abstract: The present study provides experimental evidence of in vivo reduction of genotoxic and mutagenic activities of potent carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) by the strain Lactobacillus rhamnosus Vc. In vitro studies revealed that coincubation of MNNG with viable cells of L. rhamnosus Vc resulted in the detoxification of the parent compound accompanied with reduction in genotoxicity (69%) and mutagenicity (61%) as evaluated by SOS-Chromotest and Ames test, respectively. Oral feeding of probiotic bacteria L. rhamnosus Vc (10(9) cfu) to Gallus gallus (chicks) for 30 days provided protection against MNNG-induced damage as evidenced from the significant decrease (P = 0.009) in glutathione S-transferase activity in the L. rhamnosus Vc+MNNG-treated chicks in comparison to the MNNG-treated chicks. Histopathology of colon and liver showed intact cells and mild inflammation in the L. rhamnosus Vc+MNNG-treated chicks, whereas heavy inflammation and degenerative changes were observed in MNNG-treated chicks. The results indicate that the probiotic L. rhamnosus Vc provided in vivo protection against MNNG-induced colon damage by detoxification of MNNG to less toxic metabolites.
authors: a Department of Biosciences , Saurashtra University , Rajkot , India.
authors: Sheetal P
authors: SP
authors: Pithva
authors: b Department of Biotechnology , Christ College , Rajkot , India.
authors: Padma S
authors: PS
authors: Ambalam
authors: a Department of Biosciences , Saurashtra University , Rajkot , India.
authors: Jignesh M
authors: JM
authors: Ramoliya
authors: c Shivam Vrindavan Society , Rajkot , India.
authors: Jayantilal M
authors: JM
authors: Dave
authors: a Department of Biosciences , Saurashtra University , Rajkot , India.
authors: Bharatkumar Rajiv Manuel
authors: BR
authors: Vyas
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D002645:Chickens; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008658:Inactivation, Metabolic; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D009152:Mutagenicity Tests / Q000379:methods; D019936:Probiotics / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000473:pathology
chemical_list: D016587:Antimutagenic Agents; D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D005982:Glutathione Transferase