title: Purification and biochemical characterization of 11S globulin from chan (Hyptis suaveolens L. Poit) seeds.
abstract: Chan (Hyptis suaveolens) is a Mesoamerican crop highly appreciated since the pre-Hispanic cultures. Its proteins are a good source of essential amino acids; however, there are no reports on the properties of its individual proteins. In this study, the 11S globulin (Hs11S) was purified and biochemically characterized. The molecular weight of native Hs11S was about 150-300 kDa with isoelectric points of 5.0-5.3, composed by four monomers of 53.5, 52, 51.1 and 49.5 kDa, each formed by one acidic subunit and one basic subunit linked by a disulfide bond. Dynamic light scattering, size exclusion chromatography and native PAGE show that Hs11S is assembled in different oligomeric forms. LC-MS/MS analysis confirmed its identity. Hs11S presents antigenic determinants in common with lupin 11S globulin. Carbohydrate moieties or phosphate groups linked to Hs11S were not detected. This information is very useful in order to exploit and utilize rationally chan 11S globulin in food systems.
authors: Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Los Mochis, Los Mochis, Sinaloa CP 81250, Mexico; IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, SLP CP 78216, Mexico; Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Autopista Colima-Manzanillo km 40, Tecomán, Colima CP 28100, Mexico.
authors: Esaú
authors: E
authors: Bojórquez-Velázquez
authors: Instituto de Investigación Lightbourn, Carretera las Pampas km 2.5, Cd., Jiménez, Chihuahua CP 33980, Mexico.
authors: Gisela J
authors: GJ
authors: Lino-López
authors: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, SLP CP 78216, Mexico.
authors: José A
authors: JA
authors: Huerta-Ocampo
authors: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, SLP CP 78216, Mexico.
authors: Alberto
authors: A
authors: Barrera-Pacheco
authors: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, SLP CP 78216, Mexico.
authors: Ana P
authors: AP
authors: Barba de la Rosa
authors: Instituto de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico.
authors: Abel
authors: A
authors: Moreno
authors: Tecnológico Nacional de México, Instituto Tecnológico de Tlajomulco, Carr. a San Miguel Cuyutlán km 10, Tlajomulco de Zúñiga, Jalisco CP 45650, Mexico. Electronic address: a.m.margalli@hotmail.com.
authors: N Alejandra
authors: NA
authors: Mancilla-Margalli
authors: Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Autopista Colima-Manzanillo km 40, Tecomán, Colima CP 28100, Mexico. Electronic address: osuna_juan@hotmail.com.
authors: Juan A
authors: JA
authors: Osuna-Castro
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000302:isolation & purification*; D031331:Hyptis / Q000737:chemistry*; D007526:Isoelectric Point; D008970:Molecular Weight; D055314:Seed Storage Proteins / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D005916:Globulins; D055314:Seed Storage Proteins
keywords: 11S globulin; Biochemical characterization; Dynamic light scattering; Hyptis suaveolens; Mass spectrometry; Seed storage proteins