title: Geodermatophilus sabuli sp. nov., a γ-radiation-resistant actinobacterium isolated from desert limestone.
abstract: A novel γ-radiation-resistant and Gram-staining-positive actinobacterium designated BMG 8133T was isolated from a limestone collected in the Sahara desert of Tunisia. The strain produced dry, pale-pink colonies with an optimum growth at 35–40 °C and pH 6.5–8.0. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid. MK-9(H4) was the dominant menaquinone. Galactose and glucose were detected as diagnostic sugars. The major cellular fatty acids were branched-chain saturated acids iso-C16 : 0 and iso-C15 : 0. The DNA G+C content of the novel strain was 74.5 %. The 16S rRNA gene sequence showed highest sequence identity with Geodermatophilus ruber (98.3 %). Based on phenotypic results and 16S rRNA gene sequence analysis, strain BMG 8133T is proposed to represent a novel species, Geodermatophilus sabuli sp. nov. The type strain is BMG 8133T ( = DSM 46844T = CECT 8820T).
authors: Laboratorie Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092 Tunis, Tunisia.
authors: Karima
authors: K
authors: Hezbri
authors: Laboratorie Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092 Tunis, Tunisia.
authors: Faten
authors: F
authors: Ghodhbane-Gtari
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors: Maria Del Carmen
authors: MDC
authors: Montero-Calasanz
authors: Centre National des Sciences et Technologies Nucléaires, Pôle Technologique - 2020 Sidi Thabet BP 72, Tunisia.
authors: Haïtham
authors: H
authors: Sghaier
authors: Central Facility for Microscopy, HZI - Helmholtz Centre for Infection Research Inhoffenstraße 7, 38124 Braunschweig, Germany.
authors: Manfred
authors: M
authors: Rohde
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors: Peter
authors: P
authors: Schumann
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.|School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne NE1 7RU, UK.
authors: Hans-Peter
authors: HP
authors: Klenk
authors: Laboratorie Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092 Tunis, Tunisia.
authors: Maher
authors: M
authors: Gtari
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002119:Calcium Carbonate; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003889:Desert Climate; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005720:Gamma Rays; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014416:Tunisia; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid; D002119:Calcium Carbonate