title: MiR-100 Inhibits Osteosarcoma Cell Proliferation, Migration, and Invasion and Enhances Chemosensitivity by Targeting IGFIR.
abstract: MicroRNAs are highly conserved noncoding RNA that negatively modulate protein expression at a posttranscriptional and/or translational level. MicroRNAs play an important role in the development and progression of human cancers, including osteosarcoma. Recent studies have shown that miR-100 was downregulated in many cancers; however, the role of miR-100 in human osteosarcoma has not been totally elucidated. In this study, we demonstrate that the expression of miR-100 was significantly downregulated in human osteosarcoma tissues compared to the adjacent tissues. Enforced expression of miR-100 inhibited cell proliferation, migration, and invasion abilities of osteosarcoma cells, U-2OS, and MG-63. Additionally, miR-100 also sensitized osteosarcoma cells to cisplatin and promoted apoptosis. Furthermore, overexpression of miR-100 decreased the expression of insulin-like growth factor I receptor and inhibited PI3K/AKT and MAPK/ERK signaling. In human clinical specimens, insulin-like growth factor I receptor was inversely correlated with miR-100 in osteosarcoma tissues. Collectively, our results demonstrate that miR-100 is a tumor suppressor microRNA and indicate its potential application for the treatment of osteosarcoma in future.
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China hhyyly163@163.com.
authors: Yang
authors: Y
authors: Liu
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China.
authors: Shu-Tao
authors: ST
authors: Zhu
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China.
authors: Xiao
authors: X
authors: Wang
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China.
authors: Jun
authors: J
authors: Deng
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China.
authors: Wei-Hua
authors: WH
authors: Li
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China.
authors: Peng
authors: P
authors: Zhang
authors: Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng, China.
authors: Bing-Shan
authors: BS
authors: Liu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C544266:MIRN100 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D017526:Receptor, IGF Type 1; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases
keywords: IGFIR; chemotherapy; cisplatin; miR-100; osteosarcoma