title: MDR-TB Antibody Response (Western Blot) to Fractions of Isoniazid and Rifampicin Resistant Antigens of Mycobacterium tuberculosis.
abstract: Drug-resistant TB poses a major threat to control of TB worldwide. Despite progress in the detection of Multidrug-resistant TB (MDR-TB) cases, a major diagnostic gap remains: 55% of reported TB patients estimated to have MDR-TB were not detected in 2013. MDR-TB antigens were conjugated to CNBr-activated Sepharose 4B. Specific polyclonal antibodies against MDR-TB Ags were prepared in rabbits using two boosted injections of the MDR-TB antigen. The antibodies were purified and treated with susceptible TB to remove any non-specific and cross-reactive antibodies. In the present study, comparative analysis of electrophoretic pattern of different antigens of INH/RIF-resistant TB were studied for identifying protein profiles. A RIF-resistant TB antigen was shown here to have different protein profiles from INH-resistant TB isolate. The results of Western blotting analysis showed that in the RIF- and INH-resistant antigenic fractions some bands of 14.4 and 45 kDa as immunogenic were common. Moreover, four bands of RIF-resistant TB antigen fractions (16, 19, 21, and 45 KDa) and one band of INH-resistant TB (about 26 KDa) were detected as diagnostic antigens. This study suggests that the Western blot is an accurate test to survey INH- and RIF-resistant TB antigens of M. tuberculosis infection. These findings indicate that MDR-TB diagnosis (based on Ag detection) could be useful in the identification of disease stages that precede symptomatic and microbiologically positive TB, such as subclinical and incipient TB.
authors: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.
authors: Alireza
authors: A
authors: Hadizadeh Tasbiti
authors: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.
authors: Shamsi
authors: S
authors: Yari
authors: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.|Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors: Mostafa
authors: M
authors: Ghanei
authors: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors: Mohammad Ali
authors: MA
authors: Shokrgozar
authors: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran. tbchemistry@gmail.com.
authors: Ahmadreza
authors: A
authors: Bahrmand
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D000995:Antitubercular Agents / Q000494:pharmacology*; D015153:Blotting, Western; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000276:immunology*; D011817:Rabbits; D012293:Rifampin / Q000494:pharmacology*; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin
keywords: DR-TB detection; INH- and RIF-resistant TB; MDR-TB; MDR-TB antibody response