title: Trajectories of cell-cycle progression from fixed cell populations.
abstract: An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.
authors: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.|Molecular Life Sciences, Zurich, Switzerland.
authors: Gabriele
authors: G
authors: Gut
authors: Department of Biological Sciences, Columbia University, New York, New York, USA.
authors: Michelle D
authors: MD
authors: Tadmor
authors: Department of Biological Sciences, Columbia University, New York, New York, USA.
authors: Dana
authors: D
authors: Pe'er
authors: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
authors: Lucas
authors: L
authors: Pelkmans
authors: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
authors: Prisca
authors: http://orcid.org/0000-0003-0695-6081
authors: P
authors: Liberali
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D019925:Cyclin A / Q000378:metabolism; D004261:DNA Replication; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D015203:Reproducibility of Results; D059010:Single-Cell Analysis / Q000379:methods*; D060388:Support Vector Machine; D014404:Tubulin / Q000378:metabolism
chemical_list: D019925:Cyclin A; D018809:Proliferating Cell Nuclear Antigen; D014404:Tubulin; D038362:Glycogen Synthase Kinase 3