title: Effectiveness of ultrasound, UV-C, and photocatalysis on inactivation kinetics of Aeromonas hydrophila.
abstract: In this study, bactericidal effects of 24 kHz ultrasound, ultraviolet (UV-C) irradiation, and titanium dioxide (TiO2) photocatalyst were studied on inactivation of Aeromonas hydrophila, an emerging pathogen listed on the US Environmental Protection Agency's (US EPA) candidate contaminant list. Metabolic activity (using the AlamarBlue dye) assays were performed to assess the residual activity of the microbial cells after the disinfection treatments along with culture-based methods. A faster inactivation rate of 1.52 log min(-1) and inactivation of 7.62 log10 was observed within 5 min of ultrasound exposure. Ultrasound treated cells repaired by 1.4 log10 in contrast to 5.3 log10 repair for UV-C treated cells. Ultrasound treatment significantly lowered the reactivation of Aeromonas hydrophila in comparison to UV-C- and UV-C-induced photocatalysis. Ultrasound appeared to be an effective means of inactivating Aeromonas hydrophila and could be used as a potential disinfection method for water and wastewater reuse.
authors: a Department of Biological and Agricultural Engineering , Texas A&M University , College Station , Texas , USA.
authors: Jasjeet
authors: J
authors: Kaur
authors: Raghupathy
authors: R
authors: Karthikeyan
authors: Suresh D
authors: SD
authors: Pillai
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects / Q000528:radiation effects*; D002384:Catalysis / Q000528:radiation effects; D004203:Disinfection / Q000379:methods*; D007700:Kinetics; D008027:Light; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D014025:Titanium / Q000494:pharmacology; D000069453:Ultrasonic Waves; D014466:Ultraviolet Rays; D014481:United States; D062065:Wastewater / Q000382:microbiology
chemical_list: D014025:Titanium; D062065:Wastewater; C009495:titanium dioxide
keywords: Disinfection; emerging pathogens; metabolic activity; photoreactivation; water quality