title: Metal ions doped chitosan-poly(acrylic acid) nanospheres: Synthesis and their application in simultaneously electrochemical detection of four markers of pancreatic cancer.
abstract: In this work, a one-pot method was designed to synthesize copper ions, cadmium ions, lead ions and zinc ions doped chitosan-poly(acrylic acid) nanospheres. Those nanospheres can not only produce independent electrochemical signals, but also react with glutaraldehyde (GA) to immobilize different labeled antibodies. Using the modified nanospheres as immunoprobes, a sandwich-type immunosensor was fabricated to simultaneous detection of four tumor markers (CEA, CA199, CA125 and CA242) of pancreatic cancer. This designed immunosensor exhibited good linear relationships in range from 0.1 to 100ng mL(-1) for CEA, 1 to 150UmL(-1) for CA199, CA125 and CA242, corresponding detection limits 0.02ng mL(-1), 0.4UmL(-1), 0.3UmL(-1) and 0.4UmL(-1), respectively. Meanwhile, the immunosensor was applied in analysis of clinical serum samples, whose results were well agreed with the enzyme-linked immunosorbent assay (ELISA), indicating that the proposed immunosensor gave a hope for the identification and validation of specific early cancer.
authors: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors: Qinfeng
authors: Q
authors: Rong
authors: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors: Feng
authors: F
authors: Feng
authors: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: mazhanfang@cnu.edu.cn.
authors: Zhanfang
authors: Z
authors: Ma
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques; D018394:CA-125 Antigen / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000097:blood; D048271:Chitosan / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D007477:Ions / Q000737:chemistry; D054118:Nanospheres / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000097:blood*
chemical_list: D000180:Acrylic Resins; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D002272:Carcinoembryonic Antigen; D007477:Ions; C578959:carbohydrate antigen 199, human; C065246:chitosan-poly(acrylic acid); D006046:Gold; D048271:Chitosan
keywords: Chitosan-poly(acrylic acid) nanosphere; Electrochemical immunosensor; Metal ions; Pancreatic cancer; Tumor marker