title: Development and validation of a rapid LC-MS/MS method to quantify letrozole in human plasma and its application to therapeutic drug monitoring.
abstract: A selective, rapid, and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed and validated for the determination of letrozole (LTZ) in human plasma, using anastrozole as internal standard (IS). Sample preparation was performed by one-step protein precipitation with methanol. The analyte and IS were chromatographed on a reversed-phase YMC-ODS-C18 column (2.0 × 100 mm i.d., 3 µm) with a flow rate of 0.3 mL/min. The mobile phase consisted of water containing 0.1% formic acid (v/v) and methanol containing 0.1% formic acid (v/v). The mass spectrometer was operated in selected reaction monitoring mode through electrospray ionization ion mode using the transitions of m/z 286.2 → 217.1 for LTZ and m/z 294.1 → 225.1 for IS, respectively. The method was validated for selectivity, linearity, lower limit of quantitation, precision, accuracy, matrix effects and stability in accordance with the US Food and Drug Administration guidelines. Linear calibration curves were 1.0-60.0 ng/mL. Intra- and inter-batch precision (CV) for LTZ were <9.34%, and the accuracy ranged from 97.43 to 105.17%. This method was successfully used for the analysis of samples from patients treated with LTZ in the dose of 2.5 mg/day. It might be suitable for therapeutic drug monitoring of these patients and contribute to predict the risk of adverse reactions.
authors: Center of Clinical Pharmacology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Rong
authors: R
authors: Shao
authors: Center of Clinical Pharmacology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Ling-yan
authors: LY
authors: Yu
authors: Center of Clinical Pharmacology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Hong-gang
authors: HG
authors: Lou
authors: Center of Clinical Pharmacology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Zou-rong
authors: ZR
authors: Ruan
authors: Center of Clinical Pharmacology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Bo
authors: B
authors: Jiang
authors: Center of Clinical Pharmacology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors: Jin-liang
authors: JL
authors: Chen
mesh_terms: D000077384:Anastrozole; D000970:Antineoplastic Agents / Q000097:blood*; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods*; D016903:Drug Monitoring / Q000191:economics / Q000379:methods*; D006801:Humans; D000077289:Letrozole; D057230:Limit of Detection; D009570:Nitriles / Q000097:blood* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D014230:Triazoles / Q000097:blood* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D009570:Nitriles; D014230:Triazoles; D000077384:Anastrozole; D000077289:Letrozole
keywords: LC-MS/MS; human plasma; letrozole; therapeutic drug monitoring