title: An embryonic transcriptome of the pulmonate snail Radix balthica.
abstract: The pond snail, Radix balthica (Linnaeus 1758), is an emerging model species within ecological developmental biology. While its development has been characterised in detail, genomic resources for embryonic stages are lacking. We applied Illumina MiSeq RNA-seq to RNA isolated from pools of embryos at two points during development. Embryos were cultured in either the presence or absence of predator kariomones to increase the diversity of the transcripts assembled. Sequencing produced 47.2M paired-end reads, assembled into 54,360 contigs of which 73% were successfully annotated. This transcriptome provides an invaluable resource to build a mechanistic understanding of developmental plasticity.
authors: Marine Biology and Ecology Research Centre, Marine Institute, School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: oliver.tills@plymouth.ac.uk.
authors: Oliver
authors: O
authors: Tills
authors: Marine Biology and Ecology Research Centre, Marine Institute, School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: manuela.truebanogarcia@plymouth.ac.uk.
authors: Manuela
authors: M
authors: Truebano
authors: Marine Biology and Ecology Research Centre, Marine Institute, School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: s.rundle@plymouth.ac.uk.
authors: Simon
authors: S
authors: Rundle
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012908:Snails / Q000196:embryology* / Q000235:genetics*; D059467:Transcriptome
chemical_list: D012313:RNA
keywords: Gastropod; Predator cue; Radix balthica; Transcriptome