title: KU80, a key factor for non-homologous end-joining, retards geminivirus multiplication.
abstract: KU80 is well-known as a key component of the non-homologous end-joining pathway used to repair DNA double-strand breaks. In addition, the KU80-containing DNA-dependent protein kinase complex in mammals can act as a cytoplasmic sensor for viral DNA to activate innate immune response. We have now, to our knowledge for the first time, demonstrated that the speed of a systemic infection with a plant DNA geminivirus in Arabidopsis thaliana is KU80-dependent. The early emergence of Euphorbia yellow mosaic virus DNA was significantly increased in ku80 knockout mutants compared with wild-type sibling controls. The possible impact of KU80 on geminivirus multiplication by generating non-productive viral DNAs or its role as a pattern-recognition receptor against DNA virus infection is discussed.
authors: Institute of Biomaterials and Biomolecular Systems, Department of Molecular Biology and Plant Virology, University of Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors: Kathrin S
authors: KS
authors: Richter
authors: Institute of Biomaterials and Biomolecular Systems, Department of Molecular Biology and Plant Virology, University of Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors: Holger
authors: H
authors: Jeske
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000821:virology; D059766:DNA End-Joining Repair; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D017791:Geminiviridae / Q000235:genetics / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D014779:Virus Replication
chemical_list: D029681:Arabidopsis Proteins; D004265:DNA Helicases; C469228:Ku80 protein, Arabidopsis