title: p53 immunoexpression in stepwise progression of cutaneous squamous cell carcinoma and correlation with angiogenesis and cellular proliferation.
abstract: Multistep carcinogenesis involves loss of function of tumor suppressor proteins such as p53 and induction of angiogenesis. Such mechanisms contribute to cutaneous squamous cell carcinoma progression and may be interconnected. We aimed to explore p53 immunoexpression in spectral stages of cutaneous squamous cell carcinoma and correlate expression to both neovascularization and cellular proliferation. We estimated the percentages of immunostained cells for p53 and Ki67 (proliferation marker) in three groups: 23 solar keratoses, 28 superficially invasive squamous cell carcinomas and 28 invasive squamous cell carcinomas. The Chalkley method was used to quantify the microvascular area by neoangiogenesis (CD105) immunomarker in each group. There was no significant difference for rate of p53- and Ki67-positive cells between groups. Significant positive correlation was found between the CD105 microvascular area and the rate of p53 positive cells in superficially invasive squamous cell carcinoma as well as between the rate of p53- and Ki67-positive cells in invasive squamous cell carcinoma. p53 and Ki67 immunoexpression did not increase with cutaneous squamous cell carcinoma progression. Neovascularization in the initial stage of invasion and proliferative activity in the frankly invasive stage were both associated with p53 immunoexpression. Loss of p53 tumor suppressor function through progressive steps may be directly involved in skin carcinogenesis.
authors: Department of Internal Medicine, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil. Electronic address: etienneflorence@yahoo.com.br.
authors: Michelle Etienne Baptistella
authors: ME
authors: Florence
authors: Department of Internal Medicine, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil.
authors: Juliana Yumi
authors: JY
authors: Massuda
authors: Department of Pathology, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil.
authors: Tânia Cristina Benetti
authors: TC
authors: Soares
authors: Department of Pathology, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil.
authors: Rafael Fantelli
authors: RF
authors: Stelini
authors: Department of Dermatology, University of Würzburg, Josef-Schneider-Strasse 2 Haus D8, 97080 Würzburg, Germany.
authors: Lidia Marilia
authors: LM
authors: Poppe
authors: Department of Dermatology, University of Würzburg, Josef-Schneider-Strasse 2 Haus D8, 97080 Würzburg, Germany.
authors: Eva-Bettina
authors: EB
authors: Bröcker
authors: Department of Pathology, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil.
authors: Konradin
authors: K
authors: Metze
authors: Department of Pathology, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil.
authors: Maria Letícia
authors: ML
authors: Cintra
authors: Department of Internal Medicine, University of Campinas, Rua Tessália Vieira de Camargo, 126 - Campinas, São Paulo 13083-887, Brazil.
authors: Elemir Macedo
authors: EM
authors: de Souza
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology*; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D055623:Keratosis, Actinic / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D012878:Skin Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: CD105; Carcinogenesis; Ki67; Solar keratosis; Squamous cell carcinoma; Tumor suppressor gene