title: KIR3DL1 interaction with HLA-B27 is altered by ankylosing spondylitis associated ERAP1 and enhanced by MHC class I cross-linking.
abstract: Ankylosing spondylitis (AS) is a chronic, inflammatory arthritis of the spine and peripheral joints linked to the antigen presenting molecule HLA-B27. The risk of AS is increased in patients possessing endoplasmic reticulum aminopeptidase-1 (ERAP1) polymorphisms rs30187 and rs27044 encoding amino acid changes K528R and Q730E, respectively. Dysfunction of ERAP1 is hypothesized to cause changes in expression of HLA-B27 classical (pHLA) and non-classical (FHC) conformers on antigen presenting cells (APCs), which interact with the natural killer (NK) cell receptor KIR3DL1. Dysregulation of this pathway may be pathogenic in AS. APC cell lines expressing HLA-B27 were found to inhibit cytokine production in KIR3DL1+ NK cells due to decreased APC-NK cell adhesion, and possibly activation of receptor down-regulation. Blocking pHLA and FHC reveals that both conformers inhibit cytokine production through KIR3DL1. KIR3DL1 affinity and HLA-B27 surface expression studies suggest that ERAP1 R528 and E730 expression protects from AS by generating sub-optimal pHLA, causing reduced KIR3DL1 affinity and weaker cytokine inhibition. Secondarily we observed that KIR3DL1 binding to C1R-B27 APCs is enhanced by blocking pHLA, but not FHC, raising the possibility that antibody mediated HLA-B27 cross-linking may be important in enhancing KIR3DL1+ NK cell function. This study establishes the role of both FHC and pHLA in modulating NK cell cytokine secretion and adhesion functions by interacting with KIR3DL1. This interaction varies depending on the AS association status of the ERAP1 variant expressed in APCs. Additionally antibody cross-linking of HLA-B27 enhances KIR3DL1 binding and as such could be an important pathogenic mechanism in AS.
authors: Division of Rheumatology, Department of Medicine, University of Toronto, Toronto, ON M5T 2S8, Canada.
authors: Hasan
authors: H
authors: Abdullah
authors: Toronto Western Research Institute, University Health Network, Toronto, Ontario M5T 2S8, Canada.
authors: Zhenbo
authors: Z
authors: Zhang
authors: Department of Biochemistry, McMaster University, Hamilton, Ontario L8S 4M1, Canada.
authors: Kirby
authors: K
authors: Yee
authors: Division of Rheumatology, Department of Medicine, University of Toronto, Toronto, ON M5T 2S8, Canada and Toronto Western Research Institute, University Health Network, Toronto, Ontario M5T 2S8, Canada.
authors: Nigil
authors: N
authors: Haroon
mesh_terms: D000626:Aminopeptidases / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000276:immunology; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007152:Immunologic Capping; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology; D015778:Minor Histocompatibility Antigens; D011110:Polymorphism, Genetic; D054341:Receptors, KIR3DL1 / Q000235:genetics / Q000276:immunology*; D013167:Spondylitis, Ankylosing / Q000235:genetics / Q000276:immunology* / Q000473:pathology
chemical_list: D015796:HLA-B27 Antigen; C515083:KIR3DL1 protein, human; D015778:Minor Histocompatibility Antigens; D054341:Receptors, KIR3DL1; D000626:Aminopeptidases; C464841:ERAP1 protein, human