title: Molecular characterization of bot flies, Oestrus spp., (Diptera, Oestridae), from domestic and wild Bovidae hosts.
abstract: The identification of Oestrus spp. larvae from Bovidae hosts is a difficult task due to the great morphological similarity between species. The lack of unambiguous identification criteria could have also serious epidemiological implications since domestic and wild hosts are sympatric in many natural areas. In order to accurately identify the Oestrus parasitizing hosts, we characterized two different genetic markers, 28S (rRNA) and COI, in larvae collected from domestic sheep and goats, European mouflon and Iberian ibex. Our sequence analyses demonstrate that all samples, except those from Iberian ibex, greatly resembles O. ovis and so we conclude that the species parasitizing this ibex is not O. ovis. Further studies will be needed to confirm whether it is in fact O. caucasicus, as previously suggested, or even a new species.
authors: Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain.
authors: Virginia
authors: V
authors: Moreno
authors: Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain.
authors: Ismael
authors: I
authors: Romero-Fernández
authors: Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain.
authors: Juan A
authors: JA
authors: Marchal
authors: Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain.
authors: Manuel
authors: M
authors: Beltrán
authors: Espacio Natural Sierra Nevada, Carretera Antigua de Sierra Nevada, Km 7, E-18071 Pinos Genil, Granada, Spain.
authors: José E
authors: JE
authors: Granados
authors: Department of Parasitology and Parasitic Diseases, University of Extremadura, E-10071 Cáceres, Spain.
authors: Miguel A
authors: MA
authors: Habela
authors: Transgenic Technology Research Center, Shiraz University, P.O Box 71348-74478 Shiraz, Iran.
authors: Amin
authors: A
authors: Tamadon
authors: Division of Animal Health Management, School of Veterinary Medicine, Shiraz University, P.O. Box: 71345-1731 Shiraz, Iran.
authors: Ehsan
authors: E
authors: Rakhshandehroo
authors: Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain.
authors: Mathieu
authors: M
authors: Sarasa
authors: Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain. Electronic address: jperez@ujaen.es.
authors: Jesús M
authors: JM
authors: Pérez
authors: Departamento de Biología Experimental, Universidad de Jaén, Campus Las Lagunillas, s.n., E-23071 Jaén, Spain.
authors: Antonio
authors: A
authors: Sánchez
mesh_terms: D000818:Animals; D000835:Animals, Wild; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics*; D003576:Electron Transport Complex IV; D005819:Genetic Markers; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D007492:Iran / Q000453:epidemiology; D007814:Larva / Q000235:genetics; D009198:Myiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*; D013030:Spain / Q000453:epidemiology
chemical_list: D005819:Genetic Markers; D012339:RNA, Ribosomal, 28S; D004247:DNA; D003576:Electron Transport Complex IV
keywords: 28S (rDNA); Bovidae; COI; Oestrus spp.; Phylogeny