title: Proteomic analysis of plasma from rats following total parenteral nutrition-induced liver injury.
abstract: Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially expressed in the plasma of rats following TPN-related acute liver injury. Six male rats were randomly assigned to either the saline infusion control group or the TPN infusion group. Our results demonstrate that TPN infusion in rats resulted in hepatic dysfunction and hepatocyte apoptosis. Five proteins that were differentially expressed between TPN infusion and normal rats were determined and validated in vivo. Fascinatingly, the proteomic differential displays, downregulated proteins included peroxiredoxin 2 (PRDX2), alpha-1-antiproteinase (A1AT), and fibrinogen gamma chain (FIBG), which were involved in oxidative stress, inflammatory respondence and cells apoptosis. After TPN infusion, two protein spots showed increased expression, namely, the glucagon receptor (GLR) protein and apolipoprotein A-1 (APOA1), which may mediate the effects of TPN administration on glycogen and lipid metabolism. In this study, proteomic analysis suggested TPN-related acute liver injury could be involved in limiting cellular protection mechanisms against oxidative stress-induced apoptosis. On the basis of the results, we also give molecular evidences replying TPN-related hepatitis.
authors: Division of Gastroenterology, Department of Medicine, National Yang-Ming University Hospital, I-Lan, Taiwan.|Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors: Jai-Jen
authors: JJ
authors: Tsai
authors: Department of Nursing, Chang Gung University of Science and Technology, Chronic Diseases and Health Promotion Research Center CGUST, Taiwan.|Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
authors: Hsing-Chun
authors: HC
authors: Kuo
authors: Department of Pathology, Chang Gung Memorial Hospital at Chiayi, Taiwan.
authors: Kam-Fai
authors: KF
authors: Lee
authors: Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.|School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.|Department of Education and Research, Taipei City Hospital, Taipei, Taiwan.
authors: Tung-Hu
authors: TH
authors: Tsai
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006505:Hepatitis / Q000209:etiology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D017093:Liver Failure / Q000209:etiology / Q000378:metabolism*; D008297:Male; D010289:Parenteral Nutrition, Total / Q000009:adverse effects*; D020543:Proteome / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: Animal proteomics; Peroxiredoxin 2; Total parenteral nutrition (TPN)