title: Hydrophilic modification of titania nanomaterials as a biofunctional adsorbent for selective enrichment of phosphopeptides.
abstract: TiO2-based metal oxide affinity chromatography (MOAC) nanomaterials show high potential in phosphoproteome mass-spectrometric (MS) analysis. However, a drawback of TiO2 nanomaterials is poor water solubility, which greatly reduces the enrichment efficiency of phosphopeptides and eventually limits their use in phosphoproteome MS analysis. In this work, a hydrophilic TiO2 hybrid material (denoted as NH2@TiO2) is successfully designed with 1,6-hexanediamine modified on the surface of TiO2 nanoparticles and applied as a biofunctional adsorbent for selective enrichment of phosphopeptides. The novel TiO2 hybrid material with high hydrophilicity and biocompatibility is characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and infrared (IR) spectroscopy, and its performance in selective enrichment of phosphopeptides is evaluated with the standard protein digests, human serum and the tryptic digests of nonfat milk.
authors: College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China. lhl7083609@163.com yangxinh@126.com.
authors: Hailong
authors: H
authors: Liu
authors: Tianyi
authors: T
authors: Yang
authors: Junyong
authors: J
authors: Dai
authors: Jiayu
authors: J
authors: Zhu
authors: Xiaoran
authors: X
authors: Li
authors: Rui
authors: R
authors: Wen
authors: Xinghao
authors: X
authors: Yang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D003959:Diamines / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008892:Milk / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry* / Q000378:metabolism; D059748:Proteolysis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
chemical_list: D003959:Diamines; D010748:Phosphopeptides; C009495:titanium dioxide; D014025:Titanium; D014357:Trypsin; C007297:1,6-diaminohexane