title: Characterization of the physicochemical properties of phospholipid vesicles prepared in CO2/water systems at high pressure.
abstract: Phospholipid vesicles were prepared by the nonsolvent method using high-pressure CO2/water systems. The membrane properties of vesicles prepared at different pressures and temperatures were mainly characterized based on analysis of the membrane fluidity and membrane polarity, using the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, respectively. The CO2(liquid)/water(liquid) and the CO2(supercritical)/water(liquid) two-phase (heterogeneous) systems resulted in the formation of vesicles with high yield (ca. 85%-88%). The membrane fluidity and polarity of the vesicles were similar to those of liposomes prepared by the conventional method. It is suggested that high-pressure CO2 can be used to form an appropriate hydrophobic-hydrophilic interface where phospholipid molecules as a self-assembled membrane.
authors: Department of Chemical Engineering, National Institute of Technology, Nara College, 22 Yata-cho, Yamatokoriyama, Nara 639-1080, Japan.
authors: Hidemi
authors: H
authors: Nakamura
authors: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Shogo
authors: S
authors: Taguchi
authors: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Keishi
authors: K
authors: Suga
authors: Department of Chemical Engineering, National Institute of Technology, Nara College, 22 Yata-cho, Yamatokoriyama, Nara 639-1080, Japan.
authors: Keita
authors: K
authors: Hayashi
authors: Department of Mechanical and Aerospace Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea.
authors: Ho-Sup
authors: HS
authors: Jung
authors: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Hiroshi
authors: H
authors: Umakoshi
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism*; D055598:Chemical Phenomena; D005456:Fluorescent Dyes / Q000378:metabolism; D006874:Hydrostatic Pressure; D008081:Liposomes / Q000138:chemical synthesis* / Q000302:isolation & purification*; D008560:Membrane Fluidity; D008564:Membrane Potentials; D010743:Phospholipids / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D014867:Water / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D008081:Liposomes; D010743:Phospholipids; D014867:Water; D002245:Carbon Dioxide