title: [Protective Effect of Ulinastatin against Activation of Tourniquet-Induced Platelet Mitochondria Apoptotic Signaling].
abstract: OBJECTIVE
  To investigate the protective effect of ulinastatin against the activation of tourniquet-induced platelet mitochondria apoptotic signaling.
  
  
  METHODS
  44 patients with unilateral lower limb operation and tourniquet application were randomly divided into normal saline group and ulinastatin group, and were treated with normal saline and ulinastatin respectively. 12 patents with unilateral lower limb operation but without tourniquet application were enrolled in control group. Lipid hydroperoxide (LPO) in serum was detected by LPO assay kit, the content of ATP was examined by fluorescein-luciferase assay kit; the change of mitochondrial membrane potential (Δ ψm) was detected by JC-1 mitochondrial membrane potential kit; the content of cytoplasmic cytochrome C was examined by Cytochrome C ELISA kit; Caspase-3 activity was detected by Caspase-3 fluorometric assay kit.
  
  
  RESULTS
  As compared with control group, the patients in normal saline group exhibited significant platelet mitochondrial dysfunction which characterized by low ATP level and low mitochondrial membrane potential (Δ ψm) (P < 0.05). Tourniquet application resulted in the activation of the mitochondria apoptotic signaling in platelet, displaying increase in the serum LPO level, release of mitochondrial cytochrome C into the cytoplasm, and activation of caspase-3 (P < 0.05). These alterations above-mentioned were obviously improved by ulinastatin treatment (P < 0.05).
  
  
  CONCLUSIONS
  Tourniquet induces platelet mitochondrial dysfunction and mitochondria-dependent apoptotic signaling activation, which can be improved by ulinastatin treatment.
authors: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China. E-mail: xcy11116@sina.com.
authors: Chun-Yan
authors: CY
authors: Xie
authors: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors: Jin-Fang
authors: JF
authors: Xiao
authors: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors: Zhen-Long
authors: ZL
authors: Zhao
mesh_terms: D017209:Apoptosis; D001792:Blood Platelets; D053148:Caspase 3; D045304:Cytochromes c; D006023:Glycoproteins; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria; D015398:Signal Transduction; D014111:Tourniquets
chemical_list: D006023:Glycoproteins; D045304:Cytochromes c; C505407:CASP3 protein, human; D053148:Caspase 3; C028665:urinastatin