title: Mannose-binding Lectin (MBL) as a susceptible host factor influencing Indian Visceral Leishmaniasis.
abstract: Visceral Leishmaniasis (VL), caused by Leishmania donovani is endemic in the Indian sub-continent. Mannose-binding Lectin (MBL) is a complement lectin protein that binds to the surface of Leishmania promastigotes and results in activation of the complement lectin cascade. We utilized samples of 218 VL patients and 215 healthy controls from an Indian population. MBL2 functional variants were genotyped and the circulating MBL serum levels were measured. MBL serum levels were elevated in patients compared to the healthy controls (adjusted P=0.007). The MBL2 promoter variants -78C/T and +4P/Q were significantly associated with relative protection to VL (-78C/T, OR=0.7, 95% CI=0.5-0.96, adjusted P=0.026 and +4P/Q, OR=0.66, 95% CI=0.48-0.9, adjusted P=0.012). MBL2*LYQA haplotypes occurred frequently among controls (OR=0.69, 95% CI=0.5-0.97, adjusted P=0.034). MBL recognizes Leishmania and plays a relative role in establishing L. donovani infection and subsequent disease progression. In conclusion, MBL2 functional variants were associated with VL.
authors: CSIR - Center for Cellular and Molecular Biology, Hyderabad, India.
authors: Anshuman
authors: A
authors: Mishra
authors: Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
authors: Justin S
authors: JS
authors: Antony
authors: Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
authors: Prabhanjan
authors: P
authors: Gai
authors: CSIR - Center for Cellular and Molecular Biology, Hyderabad, India.
authors: Pandarisamy
authors: P
authors: Sundaravadivel
authors: Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
authors: Tong Hoang
authors: TH
authors: Van
authors: CSIR - Center for Cellular and Molecular Biology, Hyderabad, India.
authors: Aditya Nath
authors: AN
authors: Jha
authors: CSIR - Center for Cellular and Molecular Biology, Hyderabad, India; Banaras Hindu University, Varanasi, India.
authors: Lalji
authors: L
authors: Singh
authors: Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Fondation Congolaise pour la Recherche Medicale, Brazzaville, Congo. Electronic address: velavan@medizin.uni-tuebingen.de.
authors: Thirumalaisamy P
authors: TP
authors: Velavan
authors: CSIR - Center for Cellular and Molecular Biology, Hyderabad, India.
authors: Kumarasamy
authors: K
authors: Thangaraj
mesh_terms: D016022:Case-Control Studies; D003165:Complement System Proteins / Q000276:immunology*; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007194:India; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D037601:Mannose-Binding Lectin; D003165:Complement System Proteins
keywords: India; Leishmania donovani; MBL serum level; MBL2; Visceral Leishmaniasis