title: Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae).
abstract: An aqueous extract of the haematophagous poultry ectoparasite, Dermanyssus gallinae, was subfractionated using anion exchange chromatography. Six of these subfractions were used to immunise hens and the blood from these hens was fed, in vitro, to poultry red mites. Mite mortality following these feeds was indicative of protective antigens in two of the subfractions, with the risks of mites dying being 3.1 and 3.7 times higher than in the control group (P<0.001). A combination of two-dimensional immunoblotting and immunoaffinity chromatography, using IgY from hens immunised with these subfractions, was used in concert with proteomic analyses to identify the strongest immunogenic proteins in each of these subfractions. Ten of the immunoreactive proteins were selected for assessment as vaccine candidates using the following criteria: intensity of immune recognition; likelihood of exposure of the antigen to the antibodies in a blood meal; proposed function and known vaccine potential of orthologous molecules. Recombinant versions of each of these 10 proteins were produced in Escherichia coli and were used to immunise hens. Subsequent in vitro feeding of mites on blood from these birds indicated that immunisation with Deg-SRP-1 (serpin), Deg-VIT-1 (vitellogenin), Deg-HGP-1 (hemelipoglycoprotein) or Deg-PUF-1 (a protein of unknown function) resulted in significantly increased risk of mite death (1.7-2.8times higher than in mites fed blood from control hens immunised with adjuvant only, P<0.001). The potential for using these antigens in a recombinant vaccine is discussed.
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom. Electronic address: kathryn.bartley@moredun.ac.uk.
authors: Kathryn
authors: K
authors: Bartley
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: Harry W
authors: HW
authors: Wright
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: John F
authors: JF
authors: Huntley
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: Erin D T
authors: ED
authors: Manson
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: Neil F
authors: NF
authors: Inglis
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: Kevin
authors: K
authors: McLean
authors: Biomathematics and Statistics Scotland (BioSS), The King's Buildings, Peter Guthrie Tait Road, Edinburgh EH9 3FD, United Kingdom.
authors: Mintu
authors: M
authors: Nath
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: Yvonne
authors: Y
authors: Bartley
authors: Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom.
authors: Alasdair J
authors: AJ
authors: Nisbet
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification*; D002645:Chickens; D005260:Female; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology; D008924:Mite Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D008925:Mites / Q000187:drug effects / Q000276:immunology*; D011200:Poultry; D011201:Poultry Diseases / Q000276:immunology* / Q000469:parasitology* / Q000517:prevention & control; D040901:Proteomics; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics; D015843:Serpins / Q000494:pharmacology; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D014819:Vitellogenins / Q000494:pharmacology
chemical_list: D000906:Antibodies; D000941:Antigens; C016512:IgY; D007136:Immunoglobulins; D011994:Recombinant Proteins; D015843:Serpins; D014612:Vaccines; D014819:Vitellogenins
keywords: Dermanyssus gallinae; Hemelipoglycoprotein; Serpin; Vaccine; Vitellogenin