title: Cell-block procedure in endoscopic ultrasound-guided-fine-needle-aspiration of gastrointestinal solid neoplastic lesions.
abstract: In the present review we have analyzed the clinical applications of endoscopic ultrasound-guided-fine-needle-aspiration (EUS-FNA) and the methodological aspects obtained by cell-block procedure (CBP) in the diagnostic approach to the gastrointestinal neoplastic pathology. CBP showed numerous advantages in comparison to the cytologic routine smears; in particular, better preservation of cell architecture, achievement of routine haematoxylin-eosin staining equivalent to histological slides and possibility to perform immunohistochemistry or molecular analyses represented the most evident reasons to choose this method. Moreover, by this approach, the differential diagnosis of solid gastrointestinal neoplasias may be more easily achieved and the background of contaminant non-neoplastic gastrointestinal avoided. Finally, biological samples collected by EUS-FNA CBP-assisted should be investigated in order to identify and quantify further potential molecular markers.
authors: Antonio Ieni, Valeria Barresi, Rosario Alberto Caruso, Giovanni Tuccari, Department of Human Pathology "Gaetano Barresi", Section of Anatomic Pathology, University of Messina, Azienda Ospedaliera Universitaria "Gaetano Martino", 98125 Messina, Italy.
authors: Antonio
authors: A
authors: Ieni
authors: Antonio Ieni, Valeria Barresi, Rosario Alberto Caruso, Giovanni Tuccari, Department of Human Pathology "Gaetano Barresi", Section of Anatomic Pathology, University of Messina, Azienda Ospedaliera Universitaria "Gaetano Martino", 98125 Messina, Italy.
authors: Valeria
authors: V
authors: Barresi
authors: Antonio Ieni, Valeria Barresi, Rosario Alberto Caruso, Giovanni Tuccari, Department of Human Pathology "Gaetano Barresi", Section of Anatomic Pathology, University of Messina, Azienda Ospedaliera Universitaria "Gaetano Martino", 98125 Messina, Italy.
authors: Paolo
authors: P
authors: Todaro
authors: Antonio Ieni, Valeria Barresi, Rosario Alberto Caruso, Giovanni Tuccari, Department of Human Pathology "Gaetano Barresi", Section of Anatomic Pathology, University of Messina, Azienda Ospedaliera Universitaria "Gaetano Martino", 98125 Messina, Italy.
authors: Rosario Alberto
authors: RA
authors: Caruso
authors: Antonio Ieni, Valeria Barresi, Rosario Alberto Caruso, Giovanni Tuccari, Department of Human Pathology "Gaetano Barresi", Section of Anatomic Pathology, University of Messina, Azienda Ospedaliera Universitaria "Gaetano Martino", 98125 Messina, Italy.
authors: Giovanni
authors: G
authors: Tuccari
keywords: Cell-block procedure; Diagnosis; Endoscopic ultrasound-guided-fine-needle-aspiration; Gastrointestinal tract; Immunohistochemistry