title: Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress.
abstract: Heat shock proteins (HSPs) exist extensively in eukaryotes and are conserved molecular chaperones with important contribution to plant's survival under environmental stresses. Here, the cloning and characterization of one complementary DNA (cDNA) designated as BcHSP70 from young seedlings of Brassica campestris were reported in the present work. Bioinformatic analysis revealed that BcHSP70 belongs to the plant HSP gene family and had the closest relationship with HSP70-4 from Arabidopsis thaliana. Constitutive overexpression of BcHSP70 in tobacco obviously conferred tolerance to heat stress by affecting different plant physiological parameters. In our study, transgenic tobaccos exhibited higher chlorophyll content than wild-type control when exposed to heat stress. Superoxide dismutase (SOD) and peroxidase (POD) activities, which were helpful to decrease the damage to the membrane system, were significantly higher in transformants compared to wild-type lines. Meanwhile, lower comparative electrical conductivity and malondialdehyde (MDA) content and higher proline and soluble sugar accumulation were found in transgenic tobaccos than in wild-type lines. All these above results indicated that this isolated BcHSP70 cDNA owned the ability to improve the tolerance to heat stress in transgenic tobacco, which provides helpful information and good basement to culture new robust B. campestris variety resistant to high-temperature stress by molecular breeding in the future.
authors: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, People's Republic of China.|Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
authors: Xiaorong
authors: X
authors: Wang
authors: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
authors: Bin
authors: B
authors: Yan
authors: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
authors: Min
authors: M
authors: Shi
authors: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, People's Republic of China.
authors: Wei
authors: W
authors: Zhou
authors: Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
authors: David
authors: D
authors: Zekria
authors: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, People's Republic of China.
authors: Huizhong
authors: H
authors: Wang
authors: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, People's Republic of China. guoyinkai@yahoo.com.|Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People's Republic of China. guoyinkai@yahoo.com.
authors: Guoyin
authors: G
authors: Kai
mesh_terms: D001937:Brassica / Q000235:genetics*; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics* / Q000502:physiology; D008315:Malondialdehyde / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: BcHsp70; Brassica campestris; Heat shock proteins; Heat tolerance