title: A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation.
abstract: We present a human gut metaproteomic dataset deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001573. Ten aliquots of a single stool sample collected from a healthy human volunteer were either pretreated by differential centrifugation (DC; N=5) or not centrifuged (NC; N=5). Protein extracts were then processed by filter-aided sample preparation, single-run liquid chromatography and high-resolution mass spectrometry, and peptide identification was carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform. The dataset described here is also related to the research article entitled "Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota" published in Proteomics (Tanca et al., 2015), [1].
authors: Porto Conte Ricerche, Tramariglio, Alghero, Italy.
authors: Alessandro
authors: A
authors: Tanca
authors: Porto Conte Ricerche, Tramariglio, Alghero, Italy.
authors: Antonio
authors: A
authors: Palomba
authors: Porto Conte Ricerche, Tramariglio, Alghero, Italy.
authors: Salvatore
authors: S
authors: Pisanu
authors: Porto Conte Ricerche, Tramariglio, Alghero, Italy.
authors: Maria Filippa
authors: MF
authors: Addis
authors: Porto Conte Ricerche, Tramariglio, Alghero, Italy ; Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy.
authors: Sergio
authors: S
authors: Uzzau
keywords: Differential centrifugation; Gut microbiome; Human gut; Metaproteomics; Sample preparation