title: Mitochondrial OGG1 protects against PM2.5-induced oxidative DNA damage in BEAS-2B cells.
abstract: The enzyme 8-oxoguanine glycosylase 1 (OGG1) has been shown to be involved in the repair of oxidative DNA damage. However, the effect of OGG1 on oxidative DNA damage caused by particulate matter 2.5 (PM2.5) is unknown. Herein, we demonstrated that OGG1 could inhibit the generation of ROS and alleviate mitochondrial dysfunction and increased the expression of IL-1β caused by PM2.5. The dichlorodihydrofluorescein diacetate (DCFH-DA) staining and 5,5',6,6'-tetrachloro-1,1',3,3'-. tetraethylbenzi-midazolylcarbocyanine iodide (JC-1) staining using flow cytometry showed that PM2.5 induces the generation of ROS and leads to a reduction in mitochondrial membrane potential (MMP) in BEAS-2B cells. Overexpression of OGG1 inhibited the generation of ROS and the decline in MMP. Knockdown of OGG1 by RNA interference (RNAi) increased the generation of ROS and reduced the MMP. Real-time quantitative PCR (RT-qPCR) for the mitochondrial DNA copy number (mtDNAcn) and flow cytometry for apoptosis revealed that OGG1 inhibits the apoptosis and decreases mtDNAcn induced by PM2.5. Additionally, the results of the comet assay showed that OGG1 had a significant repair effect on DNA strand breaks caused by PM2.5. Overexpression of OGG1 also significantly suppressed the expression of IL-1β caused by PM2.5. Together, these data suggest that PM2.5 leads to mitochondrial dysfunction and the up-regulation of IL-1β could be reversed by overexpression of OGG1. The mitochondrial dysfunction caused by PM2.5 could be relieved by OGG1. Thus, the base excision repair enzyme OGG1 may alleviate mitochondrial dysfunction caused by PM2.5 involved in the expression of IL-1β.
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Lawei
authors: L
authors: Yang
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Yahong
authors: Y
authors: Wang
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Ziying
authors: Z
authors: Lin
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Xu
authors: X
authors: Zhou
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Ting
authors: T
authors: Chen
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Huijuan
authors: H
authors: He
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Haili
authors: H
authors: Huang
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Teng
authors: T
authors: Yang
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China; Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Yun
authors: Y
authors: Jiang
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China; Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Wenya
authors: W
authors: Xu
authors: Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Weimin
authors: W
authors: Yao
authors: Immunology and Tumor Research Instituted, The First Affiliated Hospital, Health Science Center of Xi'an Jiaotong University, China.
authors: Tie
authors: T
authors: Liu
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China; Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China. Electronic address: gangliu11@gdmc.edu.cn.
authors: Gang
authors: G
authors: Liu
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004249:DNA Damage / Q000187:drug effects*; D045647:DNA Glycosylases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004272:DNA, Mitochondrial; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D052638:Particulate Matter / Q000009:adverse effects*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D000393:Air Pollutants; D004272:DNA, Mitochondrial; D052638:Particulate Matter; D034741:RNA, Small Interfering; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human
keywords: MMP; OGG1; PM2.5; ROS; mtDNAcn