title: Graphitic carbon nitride embedded hydrogels for enhanced gel electrophoresis.
abstract: Here, we show, for the first time, the use of graphitic carbon nitride (g-C3N4) nanosheets to improve the resolution and efficiency of protein separation in gel electrophoresis. By loading 0.04% (m/v) g-C3N4 nanosheets into the polyacrylamide gel at 25 °C, the thermal conductivity increased approximately 80% which resulted in 20% reduction in Joule heating and overall increase of separation efficiency. Also, polymerization of acrylamide occurred in the absence of tetramethylethylenediamine (TEMED) when the polyacrylamide gel contained g-C3N4 nanosheets. Hence, the g-C3N4 act simultaneously as a polymerization catalyst as well as heat sinks to lower Joule heating effect on band broadening.
authors: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, P.O. Box 91779, Iran.
authors: Mohammad
authors: M
authors: Zarei
authors: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, P.O. Box 91779, Iran.
authors: Hossein
authors: H
authors: Ahmadzadeh
authors: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, P.O. Box 91779, Iran; Center of Nano Research, Ferdowsi University of Mashhad, Mashhad, P.O. Box 91779, Iran. Electronic address: gohari@um.ac.ir.
authors: Elaheh K
authors: EK
authors: Goharshadi
authors: Department of Chemical Engineering, Ferdowsi University of Mashhad, Mashhad, P.O. Box 91779, Iran.
authors: Ali
authors: A
authors: Farzaneh
keywords: Band broadening; Gel electrophoresis; Graphitic carbon nitride; Joule heating