title: Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation.
abstract: Despite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (104 cells/cm2), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm2) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm2 exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm2. It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm2.
authors: Department of Pediatric Dentistry and Orthodontics, Araraquara School of Dentistry, Univ Estadual Paulista, Araraquara, SP, BR.
authors: Ana Paula Silveira
authors: AP
authors: Turrioni
authors: Department of Pediatric Dentistry and Orthodontics, Araraquara School of Dentistry, Univ Estadual Paulista, Araraquara, SP, BR.
authors: Liege Aldrovandi
authors: LA
authors: Montoro
authors: Department of Physiology and Pathology, Araraquara School of Dentistry, Univ Estadual Paulista, Araraquara, SP, BR.
authors: Fernanda Gonçalves
authors: FG
authors: Basso
authors: Department of Restorative Dentistry, Araraquara School of Dentistry, Univ Estadual Paulista, Araraquara, SP, BR.
authors: Leopoldina de Fátima Dantas
authors: Lde F
authors: de Almeida
authors: Department of Physiology and Pathology, Araraquara School of Dentistry, Univ Estadual Paulista, Araraquara, SP, BR.
authors: Carlos Alberto de Souza
authors: CA
authors: Costa
authors: Department of Pediatric Dentistry and Orthodontics, Araraquara School of Dentistry, Univ Estadual Paulista, Araraquara, SP, BR.
authors: Josimeri
authors: J
authors: Hebling
mesh_terms: D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007259:Infrared Rays; D013234:Stem Cells / Q000528:radiation effects*; D014070:Tooth / Q000166:cytology / Q000528:radiation effects*