title: HPV Infection in a Cohort of HIV-Positive Men and Women: Prevalence of Oncogenic Genotypes and Predictors of Mucosal Damage at Genital and Oral Sites.
abstract: The aim of this study was to assess the prevalence of HPV infection and determinants of abnormal cytology in HIV-positive patients. In a cross-sectional study, patients of both sexes, asymptomatic for HPV, underwent anorectal (men)/cervical (women) and oral swabs. Cytology and HPV-PCR detection/genotyping (high- and low-risk genotypes, HR-LR/HPV) were performed. A total of 20% of the 277 enrolled patients showed oral HPV, with no atypical cytology; in men, anal HPV prevalence was 81% with 64% HR genotypes. In women, cervical HPV prevalence was 58% with 37% HR-HPV. The most frequent genotypes were HPV-16 and HPV-18; 37% of men and 20% of women harbored multiple genotypes. Also, 47% of men showed anal squamous intraepithelial lesions (SILs); 6% had high- and 35% low-grade SILs (HSILs/LSILs); 5% had atypical squamous cells of undetermined significance (ASC-US). HR-HPV was independently associated with anal-SIL in men (P = 0.039). Moreover, 37% of women showed cervical SIL: 14 ASC-US, 15 LSILs, 4 HSILs, and 1 in situ cancer. The presence of both LR and HR-HPV in women was independently associated with SIL (P = 0.003 and P = 0.0001). HR-HPV and atypical cytology were frequently identified in our cohort. HPV screening should be mandatory in HIV-infected subjects, and vaccine programs for HPV-negative patients should be implemented.
authors: Department of Health Sciences, Institute of Infectious and Tropical Diseases, San Paolo Hospital, University of Milan, Via A Di Rudinì 8, 20142 Milan, Italy.
authors: Giulia
authors: G
authors: Marchetti
authors: Department of Health Sciences, Institute of Infectious and Tropical Diseases, San Paolo Hospital, University of Milan, Via A Di Rudinì 8, 20142 Milan, Italy.
authors: Laura
authors: L
authors: Comi
authors: Department of Health Sciences, Institute of Infectious and Tropical Diseases, San Paolo Hospital, University of Milan, Via A Di Rudinì 8, 20142 Milan, Italy.
authors: Teresa
authors: T
authors: Bini
authors: Surgery Chair, Department of Health Sciences, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Marco
authors: M
authors: Rovati
authors: Department of Health Sciences, Institute of Infectious and Tropical Diseases, San Paolo Hospital, University of Milan, Via A Di Rudinì 8, 20142 Milan, Italy.
authors: Francesca
authors: F
authors: Bai
authors: Pathology Unit, Department of Health Sciences, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Barbara
authors: B
authors: Cassani
authors: Department of Health Sciences, Institute of Obstetrics and Gynecology, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Marina
authors: M
authors: Ravizza
authors: Dentistry Chair, Department of Health Sciences, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Marco
authors: M
authors: Tarozzi
authors: Department of Health Sciences, Institute of Infectious and Tropical Diseases, San Paolo Hospital, University of Milan, Via A Di Rudinì 8, 20142 Milan, Italy.
authors: Alessandro
authors: A
authors: Pandolfo
authors: Department of Health Sciences, Institute of Obstetrics and Gynecology, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Serena
authors: S
authors: Dalzero
authors: Surgery Chair, Department of Health Sciences, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Enrico
authors: E
authors: Opocher
authors: Pathology Unit, Department of Health Sciences, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Solange
authors: S
authors: Romagnoli
authors: Dentistry Chair, Department of Health Sciences, San Paolo Hospital, University of Milan, 20142 Milan, Italy.
authors: Antonio
authors: A
authors: Carrassi
authors: Pathology Unit, Department of Medicine, Surgery and Dentistry, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20142 Milan, Italy.
authors: Silvano
authors: S
authors: Bosari
authors: Department of Health Sciences, Institute of Infectious and Tropical Diseases, San Paolo Hospital, University of Milan, Via A Di Rudinì 8, 20142 Milan, Italy.
authors: Antonella
authors: A
authors: d'Arminio Monforte