title: Biomodification of PCL Scaffolds with Matrigel, HA, and SR1 Enhances De Novo Ectopic Bone Marrow Formation Induced by rhBMP-2.
abstract: The de novo formation of ectopic bone marrow was induced using 1.2-mm-thin polycaprolactone (PCL) scaffolds biomodified with several different biomaterials. In vivo investigations of de novo bone and bone marrow formation indicated that subcutaneous implantation of PCL scaffolds coated with recombinant human bone morphogenetic protein-2 (rhBMP-2) plus Matrigel, hydroxyapatite (HA), or StemRegenin 1 (SR1) improved formation of bone and hematopoietic bone marrow as determined by microcomputed tomography, and histological and hematopoietic characterizations. Our study provides evidence that thin PCL scaffolds biomodified with Matrigel, HA, and SR1 mimic the environments of real bone and bone marrow, thereby enhancing the de novo ectopic bone marrow formation induced by rhBMP-2. This ectopic bone marrow model will serve as a unique and essential tool for basic research and for clinical applications of postnatal tissue engineering and organ regeneration.
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York. ; Cancer Research Institute, SUNY Upstate Medical University , Syracuse, New York. ; Department of Medicine, Liaoning University of Traditional Chinese Medicine , Shenyang, China .
authors: Wenjing
authors: W
authors: Bao
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York. ; Cancer Research Institute, SUNY Upstate Medical University , Syracuse, New York.
authors: Mei
authors: M
authors: Gao
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York. ; Cancer Research Institute, SUNY Upstate Medical University , Syracuse, New York. ; Department of Medicine, Liaoning University of Traditional Chinese Medicine , Shenyang, China .
authors: Yanyan
authors: Y
authors: Cheng
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York.
authors: Hyun Jae
authors: HJ
authors: Lee
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York. ; Cancer Research Institute, SUNY Upstate Medical University , Syracuse, New York.
authors: Qinghao
authors: Q
authors: Zhang
authors: Department of Radiology, SUNY Upstate Medical University , Syracuse, New York.
authors: Susan
authors: S
authors: Hemingway
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York. ; Cancer Research Institute, SUNY Upstate Medical University , Syracuse, New York. ; Department of Medicine, Liaoning University of Traditional Chinese Medicine , Shenyang, China .
authors: Zhibo
authors: Z
authors: Luo
authors: Department of Radiology, SUNY Upstate Medical University , Syracuse, New York.
authors: Andrzej
authors: A
authors: Krol
authors: Department of Medicine, Liaoning University of Traditional Chinese Medicine , Shenyang, China .
authors: Guanlin
authors: G
authors: Yang
authors: Department of Pharmacology, SUNY Upstate Medical University , Syracuse, New York. ; Cancer Research Institute, SUNY Upstate Medical University , Syracuse, New York.
authors: Jing
authors: J
authors: An
keywords: Matrigel; PCL scaffolds; de novo bone marrow formation; rhBMP-2