title: Limibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium in the family Rhodospirillaceae isolated from reclaimed land.
abstract: A Gram-stain-negative, aerobic, non-motile, non-spore-forming and short rod-shaped bacterial strain, designated CAU 1121(T), was isolated from reclaimed land in the Republic of Korea and its taxonomic position was investigated using a polyphasic approach. The bacterium grew optimally at 37 °C, at pH 6.5 and in the presence of 2% (w/v) NaCl. Based on 16S rRNA gene sequence similarity, the novel isolate belonged to the family Rhodospirillaceae within the class Alphaproteobacteria and formed an independent lineage within the evolutionary radiation encompassed by the phylum Proteobacteria. Strain CAU 1121(T) exhibited very low levels of 16S rRNA gene sequence similarity with its phylogenetic neighbours Pelagibius litoralis (similarity, 92.5%), Fodinicurvata fenggangensis (similarity, 91.4%), Fodinicurvata sediminis (similarity, 90.7%) and Tistlia consotensis (similarity, 91.0%). Strain CAU 1121(T) contained ubiquinone-10 as the only respiratory quinone and C18 : 1ω7c as the major cellular fatty acid. The DNA G+C content of the strain was 65 mol%. On the basis of phylogenetic inference, and physiological and chemotaxonomic data, it is proposed that strain CAU 1121(T) represents a novel genus and novel species in the family Rhodospirillaceae, for which the name Limibacillus halophilus gen. nov., sp. nov. is suggested. The type strain is CAU 1121(T) ( = KCTC 42420(T) = CECT 8803(T) = NBRC 110928(T)).
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors: Jong-Hwa
authors: JH
authors: Kim
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors: Maytiya
authors: M
authors: Konkit
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors: Wonyong
authors: W
authors: Kim
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012244:Rhodospirillaceae / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S