title: Paenibacillus physcomitrellae sp. nov., isolated from the moss Physcomitrella patens.
abstract: A Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain XBT, was isolated from Physcomitrella patens growing in Beijing, China. The isolate was identified as a member of the genus Paenibacillus based on phenotypic characteristics and phylogenetic inferences. The novel strain was spore-forming, motile, catalase-negative and weakly oxidase-positive. Optimal growth of strain XBT occurred at 28°C and pH 7.0-7.5. The major polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unidentified components, including one phospholipid, two aminophospholipids, three glycolipids, one aminolipid and one lipid. The predominant isoprenoid quinone was MK-7. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acid components (>5 %) were anteiso-C15 : 0 (51.2 %), anteiso-C17 : 0 (20.6 %), iso-C16 : 0 (8.3 %) and C16 : 0 (6.7 %). The G+C content of the genomic DNA was 53.3 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain XBT fell within the evolutionary distances encompassed by the genus Paenibacillus; its closest phylogenetic neighbour was Paenibacillus yonginensis DCY84T (96.6 %). Based on phenotypic, chemotaxonomic and phylogenetic properties, strain XBT is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus physcomitrellae sp. nov., is proposed. The type strain is XBT ( = CGMCC 1.15044T = DSM 29851T).
authors: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors: Xun
authors: X
authors: Zhou
authors: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors: Guan
authors: G
authors: Nan Guo
authors: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors: Le
authors: L
authors: Qi Wang
authors: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors: Su
authors: S
authors: Lan Bai
authors: New Technique Centre, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Chun
authors: C
authors: Li Li
authors: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors: Rong
authors: R
authors: Yu
authors: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors: Yan
authors: Y
authors: Hong Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D000068476:Beijing; D019068:Bryopsida / Q000382:microbiology*; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7