title: Imaging real-time HIV-1 virion fusion with FRET-based biosensors.
abstract: We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally, and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously.
authors: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.
authors: Daniel M
authors: DM
authors: Jones
authors: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.|Cellular Imaging Core, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
authors: Sergi
authors: S
authors: Padilla-Parra
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003199:Computer Systems; D031541:Fluorescence Resonance Energy Transfer; D057809:HEK293 Cells; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D014771:Virion / Q000502:physiology*; D053586:Virus Internalization