title: Possible association of killer cell immunoglobulin-like receptor genotypes and haplotypes with dry eye disease in a Han Chinese population.
abstract: OBJECTIVE
  The objective of this study was to explore whether killer immunoglobulin-like receptor (KIR) genotypes and haplotypes are associated with dry eye disease (DED) in a Han Chinese population.
  
  
  METHODS
  Polymerase chain reaction with sequence-specific primers (PCR-SSP) method was used to genotype KIR genes in 106 patients with DED and 220 healthy controls.
  
  
  RESULTS
  Twenty-three KIR genotypes were observed in the DED patient and healthy control groups, ten of which had not been described previously. The genotype G and haplotype 4 were associated with increased risk of DED, and the odds ratio (OR) and 95% confidence interval (95% CI) were 2.58, 1.10-6.02 and 2.48, 1.31-4.69, respectively; while haplotype 2 appeared to have an inverse association with the disease (OR, 0.64; 95% CI, 0.44-0.92). Genotype B/B was also associated with increased risk of DED, and the OR and 95% CI were 2.35 and 1.09-5.10, respectively. KIR haplotypes A and B have distinctive centromeric (Cen) and telomeric (Tel) gene-content motifs, and Cen-B/B was associated with increased risk of DED (OR, 2.38; 95% CI, 1.03-5.49). However, all frequencies of these KIR genotypes and haplotypes were no longer statistically significant between the two groups after the Bonferroni correction was applied for multiple testing.
  
  
  CONCLUSIONS
  There was a possible association between certain KIR genotypes and haplotypes with DED in a Han Chinese population. However, additional confirmation is required.
authors: Department of Ophthalmology, The 4 People's Hospital of Jinan, Shandong Province, P. R. China.
authors: Guifang
authors: G
authors: Ren
authors: University of Minnesota Twin Cities, Minneapolis MN.
authors: Huiyu
authors: H
authors: Li
authors: Blood Center of Shandong Province, Shandong Province, P. R. China.
authors: Wenben
authors: W
authors: Qiao
authors: Blood Center of Shandong Province, Shandong Province, P. R. China.
authors: Hua
authors: H
authors: Shen
authors: Blood Center of Shandong Province, Shandong Province, P. R. China.
authors: Yunlong
authors: Y
authors: Zhuang
authors: Department of Ophthalmology, The 4 People's Hospital of Jinan, Shandong Province, P. R. China.
authors: Tiejun
authors: T
authors: Shao
authors: Department of Ophthalmology, The 4 People's Hospital of Jinan, Shandong Province, P. R. China.
authors: Haipeng
authors: H
authors: Hu
authors: Department of Ophthalmology, The 4 People's Hospital of Jinan, Shandong Province, P. R. China.
authors: Xiuping
authors: X
authors: Zhang
mesh_terms: D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D015352:Dry Eye Syndromes / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D054340:Receptors, KIR / Q000235:genetics*; D012307:Risk Factors
chemical_list: D054340:Receptors, KIR