title: A series of strategies for solving the shortage of reference standards for multi-components determination of traditional Chinese medicine, Mahoniae Caulis as a case.
abstract: In order to solve the bottleneck of reference standards shortage for comprehensive quality control of traditional Chinese medicines (TCMs), a series of strategies, including one single reference standard to determine multi-compounds (SSDMC), quantitative analysis by standardized reference extract (QASRE), and quantitative nuclear magnetic resonance spectroscopy (qNMR) were proposed, and Mahoniae Caulis was selected as an example to develop and validate these methods for simultaneous determination of four alkaloids, columbamine, jatrorrhizine, palmatine, and berberine. Comprehensive comparisons among these methods and with the conventional external standard method (ESM) were carried out. The relative expanded uncertainty of measurement was firstly used to compare their credibility. The results showed that all these three new developed methods can accurately accomplish the quantification by using only one purified reference standard, but each of them has its own advantages and disadvantages as well as the specific application scope, which were also discussed in detail in this paper.
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Wenguang
authors: W
authors: Wang
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Xiaoli
authors: X
authors: Ma
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Xiaoyu
authors: X
authors: Guo
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Mingbo
authors: M
authors: Zhao
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Pengfei
authors: P
authors: Tu
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. Electronic address: yongjiang@bjmu.edu.cn.
authors: Yong
authors: Y
authors: Jiang
mesh_terms: D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis* / Q000592:standards; D001600:Berberine Alkaloids / Q000032:analysis / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D028143:Mahonia / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards
chemical_list: D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; C055785:jatrorrhizine; D001599:Berberine; C055786:columbamine; C005413:palmatine
keywords: One single reference standard to determine multi-compounds; Quantitative analysis by standardized reference extract; Quantitative nuclear magnetic resonance spectroscopy; Reference standards shortage; Relative expanded uncertainty of measurement; Traditional Chinese medicine