title: In vitro digestion of luteinizing hormone releasing hormone analog (LHRHa) using simulated gastric conditions in assessing human food safety.
abstract: Luteinizing hormone releasing hormone analog (LHRHa, des Gly10, [D-Ala6] ethylamide) is routinely applied for induced spawning of fish. Simulated gastric acid and pepsin were used to mimic human digestion in vitro to determine the stability of LHRHa upon possible ingestion. In vitro cleavage of LHRHa was quantified using UPLC-MS, ultra-performance liquid chromatography-mass spectrometry. LHRHa was broken down mainly into two fragments from Glp-His-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-NHEt (LHRHa) to Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-NHEt (fragment 1) and Ser-Tyr-D-Ala-Leu-Arg-Pro-NHEt (fragment 2). By 24h of incubation, LHRHa was completely digested or barely detectable if the starting material was at 250 ng/μl. If the starting concentration was at 5 ng/μl, LHRHa was completely digested by 5h of incubation, or earlier. In both extreme scenarios, the results indicated that LHRHa would be digested completely in the human stomach and would pose no risk in human food consumption. This study supports the assertion that LHRHa used as a spawning aid would not pose a human food safety risk.
authors: School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, AL 36849, USA.
authors: Baofeng
authors: B
authors: Su
authors: Department of Chemistry, Mass Spec Center, 172 Chemistry Building, Auburn University, AL 36849, USA.
authors: Yonnie
authors: Y
authors: Wu
authors: School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, AL 36849, USA.
authors: Mei
authors: M
authors: Shang
authors: Eagle Aquaculture Inc., Auburn, AL 36830, USA.
authors: Tom
authors: T
authors: Penton
authors: Eagle Aquaculture Inc., Auburn, AL 36830, USA.
authors: Sam
authors: S
authors: Lawrence
authors: School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, AL 36849, USA. Electronic address: dunhara@auburn.edu.
authors: Rex A
authors: RA
authors: Dunham
mesh_terms: D000818:Animals; D059022:Food Safety; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D007987:Gonadotropin-Releasing Hormone
keywords: Biological activity; Fish spawning aid; Food safety; In vitro digestion; LHRHa; Simulated gastric acid