title: Ube2s regulates Sox2 stability and mouse ES cell maintenance.
abstract: Sox2 has a critical role in embryonic stem (ES) cell maintenance and differentiation. Interestingly, its activity is highly dosage-dependent. Although transcriptional regulation of Sox2 has been extensively studied, the mechanisms orchestrating its degradation remain unclear. In this study, we identified ubiquitin-conjugating enzyme E2S (Ube2s) as a novel effector for Sox2 protein degradation. Ube2s mediates K11-linked polyubiquitin chain formation at the Sox2-K123 residue, thus marking it for proteasome-mediated degradation. Besides its role in fine-tuning the precise level of Sox2, Ube2s reinforces the self-renewing and pluripotent state of ES cells. Importantly, it also represses Sox2-mediated ES cell differentiation toward the neural ectodermal lineage.
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: J
authors: J
authors: Wang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Y
authors: Y
authors: Zhang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: J
authors: J
authors: Hou
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: X
authors: X
authors: Qian
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: H
authors: H
authors: Zhang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Z
authors: Z
authors: Zhang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: M
authors: M
authors: Li
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: R
authors: R
authors: Wang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: K
authors: K
authors: Liao
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Y
authors: Y
authors: Wang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Z
authors: Z
authors: Li
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: D
authors: D
authors: Zhong
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: P
authors: P
authors: Wan
authors: Department of Neurobiology, Capital Medical University, Beijing, China.
authors: L
authors: L
authors: Dong
authors: Neuroscience Research Institute, Peking University, Beijing, China.
authors: F
authors: F
authors: Liu
authors: Department of Neurobiology, Capital Medical University, Beijing, China.
authors: X
authors: X
authors: Wang
authors: Neuroscience Research Institute, Peking University, Beijing, China.
authors: Y
authors: Y
authors: Wan
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: W
authors: W
authors: Xiao
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: W W
authors: WW
authors: Zhang
mesh_terms: D000818:Animals; D064197:Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000502:physiology*; D055550:Protein Stability; D059748:Proteolysis; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes / Q000502:physiology*; D054875:Ubiquitination
chemical_list: D064197:Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C000611869:Ube2s protein, mouse; D044763:Ubiquitin-Conjugating Enzymes