title: Automated Cerebrospinal Fluid Cell Counts Using the New Body Fluid Mode of Sysmex UF-1000i.
abstract: BACKGROUND
  We evaluated the new body fluid module on Sysmex UF1000-i (UF1000i-BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid (CSF).
  
  
  METHODS
  WBC and RBC counting were compared between UF1000i-BF and Fuchs-Rosenthal counting chamber in 67 CSF samples. This study also included the evaluation of between-day precision, limit of blank (LoB), limit of detection (LoD), functional sensitivity (limit of quantitation, LoQ), carryover and linearity. Diagnostic agreement for differentiation between normal and increased WBC counts (≥5.0 × 10(6) /L) was also assessed.
  
  
  RESULTS
  The agreement between UF1000i-BF and manual WBC counts was otpiaml in all CSF samples (r = 0.99; y = 1.05x + 0.09). A modest overestimation was noticed in samples with WBC < 30 × 10(6) /L (r = 0.95; y = 1.21x - 0.15). A good agreement was observed for RBC counts (r = 0.98; y = 1.15x + 0.55), particularly in samples with RBC ≥ 18 × 10(6) /L (r = 0.98; y = 1.01x + 8.90). Between-day precision was good, with coefficient of variations (CVs) lower than 7.2% for both WBC and RBC. The LoBs were 0.1 × 10(6) WBC/L and 1.2 × 10(6) RBC/L, the LoDs were 0.7 × 10(6) WBC/L and 5.5 × 10(6) RBC/L, the LoQs were 2.4 × 10(6) WBC/L and 18.0 × 10(6) RBC/L, respectively. Linearity was excellent (r = 1.00 for both WBC and RBC). Carryover was negligible. Excellent diagnostic agreement was obtained at 4.5 × 10(6) WBC/L cut-off (sensitivity, 100%; specificity, 97.4%).
  
  
  CONCLUSIONS
  The UF1000i-BF provides rapid and accurate WBC and RBC counts in clinically relevant values of CSF cells. The use of UF1000i-BF may hence allow to replace routine optical counting, except for samples displaying abnormal WBC counts or abnormal scattergram distribution, for which differential cell counts may still be required.
authors: Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII, Bergamo, Italy. sbuoro@hpg23.it.
authors: Sabrina
authors: S
authors: Buoro
authors: Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors: Sara
authors: S
authors: Apassiti Esposito
authors: Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors: MariaGrazia
authors: M
authors: Alessio
authors: Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors: Alberto
authors: A
authors: Crippa
authors: Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy.
authors: Cosimo
authors: C
authors: Ottomano
authors: Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy.
authors: Giuseppe
authors: G
authors: Lippi
mesh_terms: D000293:Adolescent; D000328:Adult; D019540:Area Under Curve; D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods*; D001826:Body Fluids / Q000166:cytology*; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004906:Erythrocyte Count; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D016014:Linear Models; D008297:Male; D019152:Paracentesis; D055815:Young Adult
keywords: automated cell counting; body fluids Sysmex UF-1000i-BF; cerebrospinal fluid; red blood cells; white blood cells