title: Loss of histone deacetylase Hdac1 disrupts metabolic processes in intestinal epithelial cells.
abstract: By using acetyl-CoA as a substrate, acetyltransferases and histone deacetylases regulate protein acetylation by adding or removing an acetyl group on lysines. Nuclear-located Hdac1 is a regulator of intestinal homeostasis. We have previously shown that Hdac1 define specific intestinal epithelial cell basal and inflammatory-dependent gene expression patterns and control cell proliferation. We show here that Hdac1 depletion in cellulo leads to increased histone acetylation after metabolic stresses, and to metabolic disturbances resulting in impaired responses to oxidative stresses, AMPK kinase activation and mitochondrial biogenesis. Thus, nuclear Hdac1 may control intestinal epithelial cell metabolism by regulating the supply of acetyl groups.
authors: Département d'anatomie et biologie cellulaire, Faculté de médecine et des sciences de la santé, Pavillon de recherche appliquée sur le cancer, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Alexis
authors: A
authors: Gonneaud
authors: Département d'anatomie et biologie cellulaire, Faculté de médecine et des sciences de la santé, Pavillon de recherche appliquée sur le cancer, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Naomie
authors: N
authors: Turgeon
authors: Département d'anatomie et biologie cellulaire, Faculté de médecine et des sciences de la santé, Pavillon de recherche appliquée sur le cancer, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: François-Michel
authors: FM
authors: Boisvert
authors: Département d'anatomie et biologie cellulaire, Faculté de médecine et des sciences de la santé, Pavillon de recherche appliquée sur le cancer, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: François
authors: F
authors: Boudreau
authors: Département d'anatomie et biologie cellulaire, Faculté de médecine et des sciences de la santé, Pavillon de recherche appliquée sur le cancer, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada. Electronic address: Claude.Asselin@USherbrooke.ca.
authors: Claude
authors: C
authors: Asselin
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000107:Acetylation; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D056284:Histone Deacetylase 1 / Q000172:deficiency* / Q000235:genetics; D006657:Histones / Q000378:metabolism; D007422:Intestines / Q000166:cytology*; D001678:Organelle Biogenesis; D018384:Oxidative Stress; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D015398:Signal Transduction
chemical_list: D006657:Histones; D034741:RNA, Small Interfering; D055372:AMP-Activated Protein Kinases; D056284:Histone Deacetylase 1
keywords: AMPK; Acetylation; Hdac1; Intestinal epithelial cell; Mitochondria; Reactive oxygen species