title: Fut3 role in breast invasive ductal carcinoma: Investigating its gene promoter and protein expression.
abstract: Fucosylated glycans synthesized by α1,3/4-fucosyltransferase (FUT3) enzyme play an important role in breast cancer prognosis and metastasis, being involved in the binding of circulating tumor cells to the endothelium and being related to tumor stage, metastatic potential and chemoresistance. Despite the pro-tumor action of this enzyme, studies have demonstrated its role in natural killer-induced cytotoxicity through the recognition of sialyl Lewis X by C-type lectin receptors and through extrinsic apoptosis pathway triggered by Apo2L-TRAIL. This study aimed to investigate the expression pattern of FUT3 in invasive breast carcinoma (IDC) from patients of Pernambuco state, Northeast of Brazil, and genotype FUT3 promoter region to identify possible SNPs that could be associated with variations in FUT3 expression. Immunohistochemistry assay was used to access the FUT3 expression in normal (n=11) and tumor tissues (n=85). DNA sequencing was performed to genotype the FUT3 promoter region in patients with IDC (n=109) and healthy controls (n=110). Our results demonstrated that the absence of FUT3 enzyme is related to breast's IDC. The non-expression of FUT3 was more frequent in larger lesions and also in HER2 negative IDC tumors. Genomic analysis showed that two variations localized in FUT3 promoter region are possibly associated with IDC. Our results suggest that minor allele T of SNP rs73920070 (-6933 C>T) confers protection whereas minor allele T of SNP rs2306969 (-6951 C>T) triggers to susceptibility to IDC in the population of Pernambuco state, Northeast of Brazil.
authors: Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil; Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Jessica Catarine Frutuoso
authors: JC
authors: do Nascimento
authors: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Steffany de Almeida
authors: Sde A
authors: Ferreira
authors: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Juliana Lúcia de Albuquerque
authors: JL
authors: Vasconcelos
authors: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: João Luiz Quirino
authors: JL
authors: da Silva-Filho
authors: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Bruno Trajano
authors: BT
authors: Barbosa
authors: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Matheus Filgueira
authors: MF
authors: Bezerra
authors: Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil; Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Cíntia Renata Costa
authors: CR
authors: Rocha
authors: Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil; Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, CDU, Recife, PE 50670-901, Brazil.
authors: Eduardo Isidoro Carneiro
authors: EI
authors: Beltrão
mesh_terms: D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology*; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016256:Lewis X Antigen / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: D016256:Lewis X Antigen; C066693:FUT4 protein, human; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase
keywords: FucT-III; Human mammary carcinoma; Protein expression; Regulatory region; Single nucleotide polymorphism