title: Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice.
abstract: Native and designer cationic antimicrobial peptides are increasingly acknowledged as host defense molecules rather than true antimicrobials. Due to their ability to activate the innate immune system, these structures are used to treat uninfected and bacterially-infected wounds, including those harboring Acinetobacter baumannii. Previously we documented that when administered intramuscularly or topically in liquid formulations, the proline-rich host defense peptide dimer A3-APO accelerates uninfected wound re-epithelization and eliminates systemic and local A. baumannii, methicillin-resistant Staphylococcus aureus and other pathogen load from infected lesions better than conventional antibiotics. In the current study we sought to produce and characterize a novel delivery system, suitable for immediate and convenient application in non-hospital environments. The APO monomer was incorporated into polyvinyl alcohol nanofibers and the complex was polymerized into a solid patch dressing. Mice were subjected to skin abrasion where the wounds were either left uninfected or were inoculated with a near lethal dose of multidrug resistant A. baumannii strain. Analyzed after 3 days, APO monomer-containing patches improved wound appearance significantly better than polymer patches without antibiotics. When compared to colistin, the APO patches accelerated wound healing, and statistically significantly reduced wound size and wound bacterial load. The in vivo antimicrobial effect was more extensive than after intramuscular administration of the peptide drug, by using only one tenth of the active pharmaceutical ingredient. These data suggest that the APO monomer-impregnated nanofiber dressing can be developed as an economical first-line treatment option to skin injuries in general and battlefield burn and blast injuries in particular.
authors: University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hogyes Endre Street 7-9, Budapest, 1092, Hungary.
authors: Istvan
authors: I
authors: Sebe
authors: Institute of Medical Microbiology, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.|Department of Laboratory Medicine, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.
authors: Eszter
authors: E
authors: Ostorhazi
authors: Institute of Medical Microbiology, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.
authors: Aron
authors: A
authors: Fekete
authors: Department of Polymer Engineering, Faculty of Mechanical Engineering, Budapest University of Technology and Economics, Muegyetem rkp. 3., Budapest, 1111, Hungary.
authors: Krisztian N
authors: KN
authors: Kovacs
authors: University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hogyes Endre Street 7-9, Budapest, 1092, Hungary.
authors: Romana
authors: R
authors: Zelko
authors: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Ulloi ut 26, Budapest, 1085, Hungary.
authors: Ilona
authors: I
authors: Kovalszky
authors: Florey Institute of Neuroscience and Mental Health, University of Melbourne, 3010, Melbourne, VIC, Australia.
authors: Wenyi
authors: W
authors: Li
authors: Florey Institute of Neuroscience and Mental Health, University of Melbourne, 3010, Melbourne, VIC, Australia.
authors: John D
authors: JD
authors: Wade
authors: Institute of Medical Microbiology, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.
authors: Dora
authors: D
authors: Szabo
authors: Institute of Medical Microbiology, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary. otvos@temple.edu.|OLPE, LLC, 801 Mockingbird Lane, Audubon, PA, 19403, USA. otvos@temple.edu.
authors: Laszlo
authors: L
authors: Otvos
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D057139:Nanofibers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012867:Skin / Q000293:injuries / Q000382:microbiology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D004337:Drug Carriers; D011142:Polyvinyl Alcohol
keywords: Antibacterial peptide; Cutaneous infection; Nanoformulation; Topical treatment; Wound healing