title: Identification of the interaction of VP1 with GM130 which may implicate in the pathogenesis of CVB3-induced acute pancreatitis.
abstract: Coxsackievirus B3 (CVB3) is a causative agent of viral myocarditis, pancreatitis, and meningitis in humans. Although the susceptibility of CVB3-induced acute pancreatitis is age-dependent, the underlying mechanisms remain unclear. Here we identified the host factor Golgi matrix protein 130 (GM130) as a novel target of CVB3 during CVB3-induced acute pancreatitis. The viral protein VP1 interacted with GM130, disrupted GM130-GRASP65 complexes, and caused GM130 degradation, which may lead to disruption of the Golgi ribbon and development of acute pancreatitis in mice. Interestingly, the expression level of GM130 in mouse pancreas was age-dependent, which was nicely correlated with the age-associated susceptibility of CVB3-induced acute pancreatitis. Furthermore, interference RNA-mediated knockdown of GM130 significantly reduced CVB3 replication in HeLa cells. Taken together, the study identified GM130 as a novel target of CVB3, which may implicate in the pathogenesis of CVB3-induced acute pancreatitis.
authors: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors: Xiuzhen
authors: X
authors: Li
authors: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors: Yanhua
authors: Y
authors: Xia
authors: Department of Basic Medical Science, School of Medicine, University of Missouri Kansas City, Kansas City, MO, USA.
authors: Shengping
authors: S
authors: Huang
authors: Children's Hospital of Jiangxi Province, Nanchang, Jiangxi, China.
authors: Fadi
authors: F
authors: Liu
authors: Department of Pathophysiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors: Ying
authors: Y
authors: Ying
authors: Shanghai Qingpu Center for Disease Control and Prevention, Shanghai, China.
authors: Qiufang
authors: Q
authors: Xu
authors: Children's Hospital of Jiangxi Province, Nanchang, Jiangxi, China.
authors: Xin
authors: X
authors: Liu
authors: The affiliated hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.
authors: Guili
authors: G
authors: Jin
authors: Department of Basic Medical Science, School of Medicine, University of Missouri Kansas City, Kansas City, MO, USA.
authors: Christopher J
authors: CJ
authors: Papasian
authors: Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK, USA.
authors: Jack
authors: J
authors: Chen
authors: Department of Basic Medical Science, School of Medicine, University of Missouri Kansas City, Kansas City, MO, USA.
authors: Mingui
authors: M
authors: Fu
authors: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors: Xiaotian
authors: X
authors: Huang
mesh_terms: D000208:Acute Disease; D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D029822:Enterovirus B, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000150:complications / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D006056:Golgi Apparatus / Q000378:metabolism; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000378:metabolism / Q000473:pathology / Q000821:virology; D009928:Organ Specificity; D010195:Pancreatitis / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D059748:Proteolysis; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D001324:Autoantigens; C108705:GORASP1 protein, human; D000077614:Golgi Matrix Proteins; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D014764:Viral Proteins