title: Neonicotinoid insecticide interact with honeybee odorant-binding protein: Implication for olfactory dysfunction.
abstract: The decline of bee population has caused great concern in recent years. A noticeable factor points to the neonicotinoid insecticides, which remain in the nectar and pollen of plants and impair the olfactory cognition of foraging bees. However, it remains elusive that if and how neonicotinoid insecticides interact with the olfactory system of bees. Herein, we studied the binding interaction between neonicotinoid imidacloprid and ASP2, one odorant-binding protein in eastern bees, Apis cerana, by multispectroscopic methods. The results indicate that imidacloprid significantly quenched the intrinsic fluorescence of ASP2 as the static quenching mode, and expanded the conformation of ASP2 measured by the circular dichroism (CD) spectra. The acting force is mainly driven by hydrophobic force based on thermodynamic analysis. Docking analysis predicts a formation of a hydrogen bond, while the corresponding site-directed mutagenesis indicated that the hydrogen bond is not main force here. Moreover, imidacloprid with a sublethal dose (0.8ng/bee) clearly decreased the binding affinity of ASP2 to a floral volatile, β-ionone, which had been identified to strongly bind with the wild ASP2 before. This study may benefit to evaluate the effect of neonicotinoid insecticides on the olfactory cognitive behavior of bees involved in the crops pollination.
authors: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China; College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: hlli@cjlu.edu.cn.
authors: Hongliang
authors: H
authors: Li
authors: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
authors: Fan
authors: F
authors: Wu
authors: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
authors: Lei
authors: L
authors: Zhao
authors: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
authors: Jing
authors: J
authors: Tan
authors: College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors: Hongtao
authors: H
authors: Jiang
authors: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Fuliang
authors: F
authors: Hu
mesh_terms: D000595:Amino Acid Sequence; D000691:Anabasine / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001516:Bees / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology; D007306:Insecticides / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000737:chemistry / Q000494:pharmacology; D045792:Norisoprenoids / Q000737:chemistry / Q000494:pharmacology; D055696:Olfactory Perception / Q000187:drug effects; D011485:Protein Binding; D018035:Receptors, Odorant / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins; D016415:Sequence Alignment; D013057:Spectrum Analysis / Q000379:methods; D013329:Structure-Activity Relationship
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D045792:Norisoprenoids; D018035:Receptors, Odorant; D011994:Recombinant Proteins; C045648:odorant-binding protein; C082359:imidacloprid; C008157:beta-ionone; D000691:Anabasine
keywords: Binding interaction; Imidacloprid; Molecular docking; Multispectroscopy; Odorant-binding protein; Site-directed mutagenesis