title: Further evidences for the mode of action of the larvicidal m-pentadecadienyl-phenol isolated from Myracrodruon urundeuva seeds against Aedes aegypti.
abstract: Nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector Aedes aegypti. Besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. Thus, the present work aimed to assess changes in protein expression of 3rd-instar larvae of Ae. aegypti after exposure to the natural insecticide m-pentadecadienyl-phenol. Bidimensional electrophoresis followed by mass spectrometry resulted in identification of 12 proteins differentially expressed between control and treated groups. Larvae exposed to the toxic compound for 24h showed elevated detoxification response (glutathione-S-transferase), increased levels of stress-related proteins (HSP70) as well as evidence of lysosome stabilization to enable survival. Furthermore, expression of proteins involved in protection of peritrophic membrane and metabolism of lipids indicated systemic effect of toxic effects in treated larvae.
authors: Department of Biochemistry and Molecular Biology, Campus Pici, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil.
authors: Terezinha M
authors: TM
authors: Souza
authors: Department of Animal Science, Campus do Pici, Federal University of Ceara, 60455-760 Fortaleza, CE, Brazil.
authors: Erika S Bezerra
authors: ESB
authors: Menezes
authors: Department of Animal Science, Campus do Pici, Federal University of Ceara, 60455-760 Fortaleza, CE, Brazil.
authors: Rodrigo V
authors: RV
authors: Oliveira
authors: Department of Biochemistry and Molecular Biology, Campus Pici, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil.
authors: Luiz Carlos P
authors: LCP
authors: Almeida Filho
authors: Department of Animal Science, Campus do Pici, Federal University of Ceara, 60455-760 Fortaleza, CE, Brazil.
authors: Jorge M
authors: JM
authors: Martins
authors: School of Pharmacy, The University of Fortaleza, 60810-431, Fortaleza, CE, Brazil.
authors: Frederico B
authors: FB
authors: Moreno
authors: School of Pharmacy, The University of Fortaleza, 60810-431, Fortaleza, CE, Brazil.
authors: Ana Cristina O
authors: ACO
authors: Monteiro-Moreira
authors: Department of Animal Science, Campus do Pici, Federal University of Ceara, 60455-760 Fortaleza, CE, Brazil.
authors: Arlindo A Araripe
authors: AAA
authors: Moura
authors: Departament of Biology, Campus do Pici, Federal University of Ceara, 60455-760 Fortaleza, CE, Brazil. Electronic address: aurano@ufc.br.
authors: Ana F Urano
authors: AFU
authors: Carvalho
mesh_terms: D000330:Aedes / Q000187:drug effects*; D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D003715:Dengue / Q000517:prevention & control; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology; D007814:Larva / Q000187:drug effects*; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D007306:Insecticides; D010636:Phenols; D010936:Plant Extracts; C577655:m-pentadecadienyl-phenol
keywords: Comparative proteomics; Detoxification; Natural insecticide; m-Pentadecadienyl-phenol