title: [The mediation of HIF-1α siRNA to the leukocyte adhesion and myeloid cell's activity in rat's retina under early stage of diabetic retinopathy].
abstract: OBJECTIVE
  To investigate the mediation of HIF-1α siRNA to the leukocyte adhesion and myeloid cell's activity in rat's retina at early stage of diabetic retinopathy.
  
  
  METHODS
  Experimental study. HIF-1α specific siRNA expression vector pSUPERH1-siHIF1α was constructed by gene recombination. The rat diabetic model was induced by intraperitoneal injection of streptozotocin. After 2 months of diabetes induction, 27 diabetic rats were randomly chosen and assigned to 3 groups, including diabetes and phosphate buffered saline (PBS) vitreous injection group (group B), diabetes and pSUPERH1-siHIF1α vitreous transfect group (group C) and diabetes and pSUPER-retro vitreous transfect group (group D). Each group had 9 rats. Nine age matched health rats were chosen as control group (group A). Retinal leukostasis was quantified with acridine orange leukocyte fluorography. Retinal myeloid cell activity was measured by enzyme linked immunosorbent assay of myeloperoxidase (MPO). The differences of the mean values among the four groups were analyzed by one-factor analysis of variance. The multiple comparisons of the mean values among the four groups were analyzed by LSD-t analysis.
  
  
  RESULTS
  According to the results of the acridine orange leukocyte fluorography, the numbers of leukocyte adhesion in the four groups were (47.00 ± 3.60), (155.33 ± 9.01), (76.00 ± 9.05), (142.66 ± 10.26), respectively. The differences among them were significant (F = 116.25, P = 0.00). The number of leukocyte adhesion in the group C was significantly lower than that in group B (LSD-t test, P = 0.00, 95% CI: 3.56-95.10). The levels of retinal MPO in the four groups were (17.24 ± 1.13), (31.32 ± 2.53), (21.35 ± 1.06), (31.33 ± 1.26) µg/L, respectively. The differences among them were significant (F = 58.68, P = 0.00). The level of retinal MPO in the group C was significantly lower than that in group B (LSD-t test, P = 0.00, 95% CI: 6.92-13.01).
  
  
  CONCLUSIONS
  HIF-1α siRNA may play a role in the mediation of the leukocyte adhesion and myeloid cell's activity in rat's retina at early stage of diabetic retinopathy in vivo.
authors: Department of Ophthalmology, Xian No.4 Hospital, Xi'an 710004, China; Email: songhpxian@163.com.
authors: Huping
authors: H
authors: Song
authors: Qi
authors: Q
authors: Zhu
authors: Qiong
authors: Q
authors: Wu
authors: Saile
authors: S
authors: Song
authors: Hongfen
authors: H
authors: Sun
authors: Xiaoliang
authors: X
authors: Zhou
authors: Hui
authors: H
authors: Liu
mesh_terms: D000165:Acridine Orange; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000473:pathology*; D003930:Diabetic Retinopathy / Q000235:genetics / Q000473:pathology*; D005456:Fluorescent Dyes; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D007962:Leukocytes / Q000201:enzymology / Q000502:physiology*; D022423:Myeloid Cells / Q000201:enzymology / Q000502:physiology*; D009195:Peroxidase / Q000032:analysis; D034741:RNA, Small Interfering / Q000502:physiology*; D051381:Rats; D012160:Retina / Q000201:enzymology / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D034741:RNA, Small Interfering; D009195:Peroxidase; D000165:Acridine Orange