title: Immunogenicity and pharmacokinetics of short, proline-rich antimicrobial peptides.
abstract: OBJECTIVE
  The potential of proline-rich antimicrobial peptides (PrAMPs) to treat multidrug-resistant Gram-negative pathogens has been intensively investigated. They are efficacious at low doses in infection models and well tolerated in healthy mice at high doses.
  
  
  RESULTS
  PrAMPs Onc72 and Api88 were nonimmunogenic in mice unless conjugated to a carrier protein. Monoclonal IgG1/IgG2b antibodies produced by hybridoma cells were mapped to different Onc72 regions and combined in a sandwich-ELISA in a pharmacokinetic study. Onc72 was detected at concentrations up to 32 µg/ml in murine blood after administering 20 mg/kg and reached several organs within 10 min.
  
  
  CONCLUSIONS
  Both PrAMPs were not immunogenic and Onc72 concentrations in blood were well above the minimal inhibitory concentrations for Enterobacteriaceae further confirming their potential as novel antibiotics.
authors: Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.|Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
authors: Luzia
authors: L
authors: Holfeld
authors: Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.|Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
authors: Nicole
authors: N
authors: Herth
authors: Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.|Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
authors: David
authors: D
authors: Singer
authors: Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.|Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
authors: Ralf
authors: R
authors: Hoffmann
authors: Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.|Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
authors: Daniel
authors: D
authors: Knappe
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000276:immunology* / Q000493:pharmacokinetics; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000276:immunology* / Q000493:pharmacokinetics; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology; D008807:Mice, Inbred BALB C; D011392:Proline / Q000737:chemistry* / Q000276:immunology* / Q000493:pharmacokinetics
chemical_list: D000890:Anti-Infective Agents; D000911:Antibodies, Monoclonal; D023181:Antimicrobial Cationic Peptides; D000939:Epitopes; D007074:Immunoglobulin G; D011392:Proline