title: The Regulation of Muscle Structure and Metabolism by Mio/dChREBP in Drosophila.
abstract: All cells require energy to perform their specialized functions. Muscle is particularly sensitive to the availability of nutrients due to the high-energy requirement for muscle contraction. Therefore the ability of muscle cells to obtain, store and utilize energy is essential for the function of these cells. Mio, the Drosophila homolog of carbohydrate response element binding protein (ChREBP), has recently been identified as a nutrient responsive transcription factor important for triglyceride storage in the fly fat body. However, the function of Mio in muscle is unknown. In this study, we characterized the role of Mio in controlling muscle function and metabolism. Decreasing Mio levels using RNAi specifically in muscle results in increased thorax glycogen storage. Adult Mio-RNAi flies also have a flight defect due to altered myofibril shape and size in the indirect flight muscles as shown by electron microscopy. Myofibril size is also decreased in flies just before emerging from their pupal cases, suggesting a role for Mio in myofibril development. Together, these data indicate a novel role for Mio in controlling muscle structure and metabolism and may provide a molecular link between nutrient availability and muscle function.
authors: Department of Biology, Hofstra University, Hempstead, NY, 11549, United States of America.
authors: Grzegorz L
authors: GL
authors: Polak
authors: Department of Biology, Hofstra University, Hempstead, NY, 11549, United States of America.
authors: Anthony
authors: A
authors: Pasqualino
authors: Department of Biology, Hofstra University, Hempstead, NY, 11549, United States of America.
authors: James E B
authors: JE
authors: Docherty
authors: Department of Biology, Nassau Community College, Garden City, NY, 11530, United States of America.
authors: Stephen J
authors: SJ
authors: Beck
authors: Department of Biology, Hofstra University, Hempstead, NY, 11549, United States of America; Division of Science, Penn State Berks, Reading, PA, 19610, United States of America.
authors: Justin R
authors: JR
authors: DiAngelo
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005426:Flight, Animal / Q000502:physiology; D017344:Genes, Insect; D006003:Glycogen / Q000378:metabolism; D009132:Muscles / Q000033:anatomy & histology* / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C484002:mio protein, Drosophila; D006003:Glycogen