title: Simultaneous determination of hyzetimibe and its main active metabolite in plasma by LC-MS/MS and its application in PK study.
abstract: BACKGROUND
  Hyzetimibe is a new compound belonging to a novel class of selective cholesterol absorption inhibitors. A simple, highly sensitive LC-MS/MS method has been developed for the quantification of hyzetimibe and its main active metabolite, hyzetimibe-glucuronide, in human plasma.
  
  
  RESULTS
  Analytical samples were prepared using a protein precipitation method coupled with a concentration process. The linearity of this method was established for concentrations in the ranges of 0.05-50 and 0.5-500 ng/ml for hyzetimibe and hyzetimibe-glucuronide, respectively. The accuracy and precision of the method varied from 97.9 to 105% and 2.6 to 7.4%, respectively.
  
  
  CONCLUSIONS
  This study represents the first reported example of an LC-MS/MS assay for the simultaneous quantification of hyzetimibe and its main active metabolite, hyzetimibe-glucuronide, in human plasma. Furthermore, this method has been successfully applied to a PK study.
authors: Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Jinliang
authors: J
authors: Chen
authors: Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Honggang
authors: H
authors: Lou
authors: Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Bo
authors: B
authors: Jiang
authors: Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Rong
authors: R
authors: Shao
authors: Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Zourong
authors: Z
authors: Ruan
authors: Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Jian'an
authors: J
authors: Wang
mesh_terms: D000924:Anticholesteremic Agents / Q000737:chemistry* / Q000378:metabolism; D001385:Azetines / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D005464:Fluorobenzenes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C000597911:1-(4-fluorophenyl)-3-(3-(4-fluorophenyl)-4-hydroxybut-2-enyl)-4-(4-hydroxyphenyl)-2-azetidinone; D000924:Anticholesteremic Agents; D001385:Azetines; D005464:Fluorobenzenes