title: A biospectroscopic analysis of human prostate tissue obtained from different time periods points to a trans-generational alteration in spectral phenotype.
abstract: Prostate cancer is the most commonly-diagnosed malignancy in males worldwide; however, there is marked geographic variation in incidence that may be associated with a Westernised lifestyle. We set out to determine whether attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy combined with principal component analysis-linear discriminant analysis or variable selection techniques employing genetic algorithm or successive projection algorithm could be utilised to explore differences between prostate tissues from differing years. In total, 156 prostate tissues from transurethral resection of the prostate procedures for benign prostatic hyperplasia from 1983 to 2013 were collected. These were distributed to form seven categories: 1983-1984 (n = 20), 1988-1989 (n = 25), 1993-1994 (n = 21), 1998-1999 (n = 21), 2003-2004 (n = 21), 2008-2009 (n = 20) and 2012-2013 (n = 21). Ten-μm-thick tissue sections were floated onto Low-E (IR-reflective) slides for ATR-FTIR or Raman spectroscopy. The prostate tissue spectral phenotype altered in a temporal fashion. Examination of the two categories that are at least one generation (30 years) apart indicated highly-significant segregation, especially in spectral regions containing DNA and RNA bands (≈1,000-1,490 cm(-1)). This may point towards alterations that have occurred through genotoxicity or through epigenetic modifications. Immunohistochemical studies for global DNA methylation supported this. This study points to a trans-generational phenotypic change in human prostate.
authors: Centre for Biophotonics, LEC, Lancaster University, Lancaster LA1 4YQ, UK.|Department of Obstetrics and Gynaecology, Central Lancashire Teaching Hospitals NHS Foundation Trust, Preston, UK.
authors: Georgios
authors: G
authors: Theophilou
authors: Centre for Biophotonics, LEC, Lancaster University, Lancaster LA1 4YQ, UK.|Institute of Chemistry, Biological Chemistry and Chemometrics, Federal University of Rio Grande do Norte, Natal 59072-970, RN-Brazil.
authors: Kássio M G
authors: KM
authors: Lima
authors: Department of Obstetrics and Gynaecology, Central Lancashire Teaching Hospitals NHS Foundation Trust, Preston, UK.
authors: Matthew
authors: M
authors: Briggs
authors: Centre for Biophotonics, LEC, Lancaster University, Lancaster LA1 4YQ, UK.|Department of Obstetrics and Gynaecology, Central Lancashire Teaching Hospitals NHS Foundation Trust, Preston, UK.
authors: Pierre L
authors: PL
authors: Martin-Hirsch
authors: Department of Obstetrics and Gynaecology, Central Lancashire Teaching Hospitals NHS Foundation Trust, Preston, UK.
authors: Helen F
authors: HF
authors: Stringfellow
authors: Centre for Biophotonics, LEC, Lancaster University, Lancaster LA1 4YQ, UK.
authors: Francis L
authors: FL
authors: Martin
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism; D019992:Databases as Topic; D016002:Discriminant Analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010641:Phenotype; D025341:Principal Component Analysis; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013997:Time Factors
chemical_list: D044503:5-Methylcytosine