title: Description of Bartonella ancashensis sp. nov., isolated from the blood of two patients with verruga peruana.
abstract: Three novel isolates of the genus Bartonella were recovered from the blood of two patients enrolled in a clinical trial for the treatment of chronic stage Bartonella bacilliformis infection (verruga peruana) in Caraz, Ancash, Peru. The isolates were initially characterized by sequencing a fragment of the gltA gene, and found to be disparate from B. bacilliformis. The isolates were further characterized using phenotypic and genotypic methods, and found to be genetically identical to each other for the genes assessed, but distinct from any known species of the genus Bartonella, including the closest relative B. bacilliformis. Other characteristics of the isolates, including their morphology, microscopic and biochemical properties, and growth patterns, were consistent with members of the genus Bartonella. Based on these results, we conclude that these three isolates are members of a novel species of the genus Bartonella for which we propose the name Bartonella ancashensis sp. nov. (type strain 20.00T = ATCC BAA-2694T = DSM 29364T).
authors: Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.|U.S. Naval Medical Research Center, Silver Spring, MD 20910, USA.
authors: Kristin E
authors: KE
authors: Mullins
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Jun
authors: J
authors: Hang
authors: U.S. Naval Medical Research Center, Silver Spring, MD 20910, USA.
authors: Ju
authors: J
authors: Jiang
authors: U.S. Naval Medical Research Unit No. 6, Lima, Peru.
authors: Mariana
authors: M
authors: Leguia
authors: U.S. Naval Medical Research Unit No. 6, Lima, Peru.
authors: Matthew R
authors: MR
authors: Kasper
authors: Instituto de Medicina Tropical Alexander von Humboldt-Universidad Peruana Cayetano Heredia, Lima, Peru.
authors: Palmira
authors: P
authors: Ventosilla
authors: Instituto de Medicina Tropical Alexander von Humboldt-Universidad Peruana Cayetano Heredia, Lima, Peru.
authors: Ciro
authors: C
authors: Maguiña
authors: Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
authors: Richard G
authors: RG
authors: Jarman
authors: Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
authors: David
authors: D
authors: Blazes
authors: Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.|U.S. Naval Medical Research Center, Silver Spring, MD 20910, USA.
authors: Allen L
authors: AL
authors: Richards
mesh_terms: D015373:Bacterial Typing Techniques; D001473:Bartonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001474:Bartonella Infections / Q000097:blood / Q000382:microbiology*; D001482:Base Composition; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010568:Peru; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S