title: Broad spectrum antimicrobial compounds from the bacterium Exiguobacterium mexicanum MSSRFS9.
abstract: Clinical bacterial pathogens front a major challenge for the clinical researchers and physicians. In particular microbial pathogens like Escherichia coli, Shigella flexneri, Klebsiella pneumonia and Salmonella enterica are apparelled with systemic machineries to bring down the human immune system as well as proliferate dramatically in a short period which in turn cause a pronounced ailment to the human health. In vitro evaluation of four purified compounds isolated from rhizosphere bacterium Exiguobacterium mexicanum tested against clinical pathogens mentioned above by disc diffusion method showed the two compounds viz., 3,6,18-trione, 9,10-dihydro-12'-hydroxyl-2methyl-5-(phenyl methyl) (5'-alpha, 10-alpha)-dihydroergotamine (C3) and dipropyl - S-propyl ester (C4) exhibit antibacterial property against all the tested pathogens. Among the four clinical pathogens tested, compound C3 has shown higher zone of inhibition against S. enterica with 17±0 mm, followed by S. flexneri with 16.5±0.7 mm, E. coli with 15±0 mm and K. pneumoniae with 14±0 mm, respectively. The compound C4 has shown higher antimicrobial activity against S. enterica with 21.5±0.7 mm zone of inhibition, followed by S. flexneri with 19.5±0.7 mm, E. coli with 17±0 mm and K. pneumoniae with 16±0 mm, these two compounds were found to be safer when subjected to rat haematological and enzymatic analysis.
authors: Laboratory Integrated Pest Management (IPM), M.S. Swaminathan Research Foundation, III Cross Road, Institutional Area, Taramani, Chennai 600113, Tamil Nadu, India. Electronic address: spshanthakumar@gmail.com.
authors: S P
authors: SP
authors: Shanthakumar
authors: Laboratory Integrated Pest Management (IPM), M.S. Swaminathan Research Foundation, III Cross Road, Institutional Area, Taramani, Chennai 600113, Tamil Nadu, India.
authors: Purushothaman
authors: P
authors: Duraisamy
authors: Laboratory Integrated Pest Management (IPM), M.S. Swaminathan Research Foundation, III Cross Road, Institutional Area, Taramani, Chennai 600113, Tamil Nadu, India.
authors: Ganga
authors: G
authors: Vishwanath
authors: Cell and Experimental Pathology, Department Laboratory Medicine, Lund University, Clinical Research Centre, Jan Waldenströms gata 35, Bldg 91, fl 11, Skåne University Hospital, SE 205 02 Malmö, Sweden.
authors: Benson Chellakkan
authors: BC
authors: Selvanesan
authors: Sri Ramachandra Medical College & Research Institute, Chennai 600116, India.
authors: Vijayakumar
authors: V
authors: Ramaraj
authors: Scientific and Advisory Board, IIBAT, Padappai, Kanchipuram Dt, Tamil Nadu, India.
authors: B
authors: B
authors: Vasantharaj David
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000302:isolation & purification* / Q000494:pharmacology*; D056449:Bacillales / Q000737:chemistry*; D001688:Biological Products / Q000008:administration & dosage / Q000009:adverse effects / Q000302:isolation & purification* / Q000494:pharmacology*; D001773:Blood Cells / Q000187:drug effects; D052978:Disk Diffusion Antimicrobial Tests; D004755:Enterobacteriaceae / Q000187:drug effects*; D006454:Hemoglobins / Q000032:analysis; D008958:Models, Molecular; D015394:Molecular Structure; D051381:Rats
chemical_list: D000890:Anti-Infective Agents; D001688:Biological Products; D006454:Hemoglobins
keywords: Antibacterial compound; Bioassay; Pathogen; Spectral analysis; Toxicology