title: Detection of DNA double-strand breaks in boron neutron capture reaction.
abstract: We evaluated DNA double-strand breaks (DSBs) induced by boron neutron capture reaction (BNCR) using plasmid DNA, boron solution, and gel electrophoresis. The amount of the linear form of DNA produced by DSBs increased with the neutron-beam irradiation dose. The amount of the open-circular form of DNA produced by single-strand breaks (SSBs) increased with the neutron-beam irradiation dose and the (10)B concentration. The model facilitated quantification of BNCR-induced DSBs and SSBs, irrespective of the DNA repair mechanism.
authors: Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan; Doctoral Program in Clinical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors: Emiko
authors: E
authors: Okamoto
authors: Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors: Tetsuya
authors: T
authors: Yamamoto
authors: Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan. Electronic address: knakai@md.tsukuba.ac.jp.
authors: Kei
authors: K
authors: Nakai
authors: Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors: Fumiyo Yoshida
authors: Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors: Akira
authors: A
authors: Matsumura
mesh_terms: D016754:Boron Neutron Capture Therapy; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel
keywords: DNA damage; Double-strand break; Neutron capture therapy