title: Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion.
abstract: Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384.
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. ping.chengcheng@163.com.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. ping.chengcheng@163.com.
authors: Nana
authors: N
authors: Ping
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. qiuhuiying@suda.edu.cn.
authors: Huiying
authors: H
authors: Qiu
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. shirley-1214@163.com.
authors: Qian
authors: Q
authors: Wang
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. daihaiping8@126.com.
authors: Haiping
authors: H
authors: Dai
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. changgengruan@hotmail.com.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. changgengruan@hotmail.com.
authors: Changgeng
authors: C
authors: Ruan
authors: DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. Stefan.Ehrentraut@dsmz.de.
authors: Stefan
authors: S
authors: Ehrentraut
authors: DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. hdr@dsmz.de.
authors: Hans G
authors: HG
authors: Drexler
authors: DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. rml@dsmz.de.
authors: Roderick A F
authors: RA
authors: MacLeod
authors: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. chensuning@suda.edu.cn.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. chensuning@suda.edu.cn.
authors: Suning
authors: S
authors: Chen
mesh_terms: D045744:Cell Line, Tumor; D050881:E1A-Associated p300 Protein / Q000235:genetics*; D006801:Humans; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D014157:Transcription Factors; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human