title: Topical Application of Angelica sinensis Improves Pruritus and Skin Inflammation in Mice with Atopic Dermatitis-Like Symptoms.
abstract: Angelica sinensis (AS) is one of the most popular medicinal foods used as a hematopoietic herb and also traditionally applied topically for skin disorders. However, the effectiveness of AS on atopic dermatitis (AD) has not been reported yet. This study was conducted to evaluate the antipruritic and anti-inflammatory effects of AS on regulating AD-related mediators in DNCB (2,4-dinitrochlorobenzene)-induced mice. AS was topically applied to the dorsal skin of DNCB-challenged mice for 11 days. Alteration of skin thickness was measured for assessment of histological improvement. In addition, the number of mast cells, the level of serum immunoglobulin E (IgE), the counting of scratching behavior, and the expression of substance P were evaluated. Also, the expressions of cytokines, nuclear factor κB (NF-κB), phospho-IκBα, and mitogen-activated protein kinases (MAPKs) were measured for evaluating the improvement of skin inflammation. The repeated treatment of AS significantly inhibited the skin thickness, the number of mast cells, and the level of serum IgE. Moreover, AS significantly suppressed the increased scratching behavior and the expression of substance P compared to the DNCB group. Topical application of AS also reduced the level of cytokines (IL-4, IL-6, TNF-α, and IFN-γ) as well as the expressions of NF-κB, phospho-IκBα, and phospho-MAPKs in the dorsal skin. The results of our study suggest that topical application of AS might have efficacy for modulating pruritus and inflammation in AD. Further studies are required to further characterize the mechanism of actions of AS.
authors: 1 Department of Convergence Korean Medical Science, College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
authors: Jaehong
authors: J
authors: Lee
authors: 1 Department of Convergence Korean Medical Science, College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
authors: You Yeon
authors: YY
authors: Choi
authors: 1 Department of Convergence Korean Medical Science, College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
authors: Mi Hye
authors: MH
authors: Kim
authors: 1 Department of Convergence Korean Medical Science, College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
authors: Jae Min
authors: JM
authors: Han
authors: 1 Department of Convergence Korean Medical Science, College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
authors: Ji Eun
authors: JE
authors: Lee
authors: 2 College of Pharmacy, Kyung Hee University , Seoul, Republic of Korea.
authors: Eun Hye
authors: EH
authors: Kim
authors: 2 College of Pharmacy, Kyung Hee University , Seoul, Republic of Korea.
authors: Jongki
authors: J
authors: Hong
authors: 3 Department of Oriental Physiology, College of Pharmacy, Kyung Hee University , Seoul, Republic of Korea.
authors: Jinju
authors: J
authors: Kim
authors: 1 Department of Convergence Korean Medical Science, College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
authors: Woong Mo
authors: WM
authors: Yang
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage*; D011537:Pruritus / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D012867:Skin / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007073:Immunoglobulin E
keywords: Angelica sinensis; anti-inflammation; atopic dermatitis; pruritus