title: In vitro amylolysis of pulse and hylon VII starches explained in terms of their composition, morphology, granule architecture and interaction between hydrolysed starch chains.
abstract: The objective of this study was to understand the factors underlying the susceptibility of pulse (lablab bean, navy bean, rice bean, tepary bean, velvet bean, and wrinkled pea) and hylon VII starches towards in vitro hydrolysis by the combined action of pancreatin and amyloglucosidase. The time taken to reach an equivalent level of hydrolysis (50%) varied significantly among the starches. Changes to molecular order, crystallinity, double helical content, radial orientation of starch chains (polarized light), enthalpy and apparent amylose content during the progress of hydrolysis showed that rate and extent of hydrolysis were influenced both by the structure of the native starches at different levels (molecular, mesoscopic, microscopic) of granule organization, and by the extent of retrogradation between hydrolysed starch chains.
authors: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.
authors: S
authors: S
authors: Maaran
authors: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada. Electronic address: rhoover@mun.ca.
authors: R
authors: R
authors: Hoover
authors: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada.
authors: V
authors: V
authors: Vamadevan
authors: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada.
authors: R N
authors: RN
authors: Waduge
authors: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada.
authors: Q
authors: Q
authors: Liu
mesh_terms: D000688:Amylose / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D010194:Pancreatin / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013213:Starch / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D010194:Pancreatin; D013213:Starch; D000688:Amylose; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Amylolysis; Morphology; Pulse starches; Rate and extent of hydrolysis; Structure