title: Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells.
abstract: Human placental extract (HPE) is widely used in Korea to relieve fatigue. However, its effects on human dermal papilla cells (hDPCs) remain unknown. In the present study, in an effort to develop novel therapies to promote hair growth, we screened HPE. We demonstrate that HPE has hair growth‑promoting activities and induces β‑catenin expression through the inhibition of glycogen synthase kinase‑3β (GSK‑3β) by phosphorylation in hDPCs. Treatment with HPE significantly increased the viability of the hDPCs in a concentration‑dependent manner, as shown by bromodeoxyuridine (BrdU) assay. HPE also significantly increased the alkaline phosphatase (ALP) expression levels. The increased β‑catenin levels and the inhibition of GSK‑3β (Ser9) by phosphorylation suggested that HPE promoted the hair-inductive capacity of hDPCs. We compared the effects of treatment with HPE alone and treatment with HPE in conjunction with minoxidil (MXD). We found that HPE plus MXD effectively inhibited GSK‑3β by phosphorylation (Ser9) in the hDPCs. Moreover, we demonstrated that HPE was effective in inducing root hair elongation in rat vibrissa hair follicles, and that treatment with HPE led to a delay in catagen progression. Overall, our findings suggest that HPE promotes hair growth and may thus provide the basis of a novel therapeutic strategy for the clinical treatment of hair loss.
authors: Major in Biomedical Science, Department of Medicine, Graduate School, Chung‑Ang University, Seoul 156‑755, Republic of Korea.
authors: Tae-Rin
authors: TR
authors: Kwon
authors: Major in Biomedical Science, Department of Medicine, Graduate School, Chung‑Ang University, Seoul 156‑755, Republic of Korea.
authors: Chang Taek
authors: CT
authors: Oh
authors: Major in Biomedical Science, Department of Medicine, Graduate School, Chung‑Ang University, Seoul 156‑755, Republic of Korea.
authors: Eun Ja
authors: EJ
authors: Choi
authors: Green Cross Corporation, Giheung‑gu, Yongin-si, Gyeonggi-do 446-770, Republic of Korea.
authors: Hye Min
authors: HM
authors: Park
authors: Green Cross Corporation, Giheung‑gu, Yongin-si, Gyeonggi-do 446-770, Republic of Korea.
authors: Hae Jung
authors: HJ
authors: Han
authors: Green Cross Corporation, Giheung‑gu, Yongin-si, Gyeonggi-do 446-770, Republic of Korea.
authors: Hyi Jeong
authors: HJ
authors: Ji
authors: Major in Biomedical Science, Department of Medicine, Graduate School, Chung‑Ang University, Seoul 156‑755, Republic of Korea.
authors: Beom Joon
authors: BJ
authors: Kim
mesh_terms: D000818:Animals; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000201:enzymology*; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D018859:Hair Follicle / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D007039:Hypotrichosis / Q000188:drug therapy / Q000201:enzymology; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D045424:Complex Mixtures; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3