title: A Well-Controlled Nucleus Pulposus Tissue Culture System with Injection Port for Evaluating Regenerative Therapies.
abstract: In vitro evaluation of nucleus pulposus (NP) tissue regeneration would be useful, but current systems for NP culture are not ideal for injections. The aim of this study was to develop a long-term culture system for NP tissue that allows injections of regenerative agents. Bovine caudal NPs were harvested and placed in the newly designed culture system. After equilibration of the tissue to 0.3 MPa the volume was fixed and the tissue was cultured for 28 days. The cell viability and extracellular matrix composition remained unchanged during the culture period and gene expression profiles were similar to those obtained in earlier studies. Furthermore, to test the responsiveness of bovine caudal NPs in the system, samples were cultured for 4 days and injected twice (day 1 and 3) with (1) PBS, (2) Link-N, for regeneration, and (3) TNF-α, for degeneration. It was shown that TNF-α increased COX2 gene expression, whereas no effect of Link-N was detected. In conclusion, the newly designed system allows long-term culture of NP tissue, wherein tissue reactions to injected stimulants can be observed.
authors: Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven, The Netherlands.
authors: Irene T M
authors: IT
authors: Arkesteijn
authors: Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven, The Netherlands.|Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Vivian H M
authors: VH
authors: Mouser
authors: Division of Orthopaedic Surgery, Lady Davis Institute for Medical Research, McGill University, Montreal, Canada.
authors: Fackson
authors: F
authors: Mwale
authors: Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven, The Netherlands. b.g.m.v.dijk@tue.nl.
authors: Bart G M
authors: BG
authors: van Dijk
authors: Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven, The Netherlands.|Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Keita
authors: K
authors: Ito
mesh_terms: D000818:Animals; D002417:Cattle; D007403:Intervertebral Disc; D044968:Regenerative Medicine / Q000379:methods*; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods*
keywords: In vitro; Intervertebral disc; Link-N; Regeneration; TNF-α