title: MicroRNA-126 Overexpression Inhibits Proliferation and Invasion in Osteosarcoma Cells.
abstract: This study investigated the biological effects of microRNA-126 overexpression in human MG63 osteosarcoma cells. A recombinant plasmid expressing microRNA-126, pcDNA6.2-microRNA-126, was constructed and transfected into MG63 cells. Using real-time fluorogenic quantitative polymerase chain reaction, the microRNA-126 expression was measured in microRNA-126-MG63 group, Ctrl-MG63 group, and blank group. Cell proliferation, cell cycle distribution, cell migration, and invasion were analyzed using methyl thiazolyl tetrazolium assay, flow cytometer, wound-healing assay, and transwell assay, respectively. As expected, microRNA-126 expression was higher in microRNA-126-MG63 group than in Ctrl-MG63 group and blank group (both P < .05). After 48/72 hours of transfection, cell proliferation in microRNA-126-MG63 group was significantly reduced compared to blank group (both P < .05). Compared to blank group, cell population in G0/G1 stage was significantly higher in microRNA-126-MG63 group, accompanied by lower cell numbers in the S and G2/M phases and decreased proliferation index (all P < .05). Wound-healing assay showed a wider scratch width in microRNA-126-MG63 group and reduced cell migration than blank group (both P < .05). Cells overexpressing microRNA-126 exhibited reduced ADAM9 expression levels compared to other 2 groups (all P < .05), suggesting ADAM9 is a target of microRNA-126. Cell proliferation, migration, and invasion rates were reduced in microRNA-126 group after 48/72 hours of transfection, compared with blank group (all P < .05). Cotransfection of pcDNA6.2-microRNA-126 and pMIR-ADAM9 into MG63 cells led to higher cell proliferation, invasion, and migration rates, compared with transfection of pcDNA6.2-microRNA-126 alone (all P < .05). In summary, our data show that microRNA-126 inhibits cell proliferation, migration, and invasion in human osteosarcoma cells by targeting ADAM9.
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China.
authors: Shuaihua
authors: S
authors: Wang
authors: Department of Ophthalmology, Linyi People's Hospital Affiliated to Shandong University, Shandong, P.R. China.
authors: Xinlei
authors: X
authors: Wang
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China guoqiang_0526@163.com hewen1028@126.com.
authors: Qiang
authors: Q
authors: Guo
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China.
authors: Guilong
authors: G
authors: Wang
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China.
authors: Xiangzhen
authors: X
authors: Han
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China.
authors: Xiao
authors: X
authors: Li
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China.
authors: Zuo-Wei
authors: ZW
authors: Shi
authors: Department of Pediatric Orthopedics and Bone Oncology, Linyi People's Hospital Affiliated to Shandong University, Linyi, Lanshan, Shandong, P.R. China.
authors: Wen
authors: W
authors: He
mesh_terms: D020413:3' Untranslated Regions; D051722:ADAM Proteins / Q000235:genetics; D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics*; D034622:RNA Interference; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C527111:MIRN126 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D051722:ADAM Proteins; C098339:ADAM9 protein, human
keywords: ADAM9; MG63 cells; eukaryotic expression vector; microRNA-126; migration; osteosarcoma; proliferation