title: [Growth and survival of total and pathogenic Vibrio parahaemolyticus in American oyster (Crassostrea virginica) under cold storage].
abstract: OBJECTIVE
  To quantify Vibrio parahaemolyticus densities in American oyster (Crassostrea virginica) under cold storage.
  
  
  METHODS
  320 oysters were stored at 7°C for nine days and total and pathogenic densities were determined by the NMP-PCR methodology.
  
  
  RESULTS
  V. parahaemolyticus tlh+ densities were observed on 0,3, and 6 days of storage at 1.134, 2.764 and 0.785 log10NMP/g, respectively, and pathogenic density trh+ on 0 and 3 days at 0.477 and 0.519 log10NMP/g, respectively; the pathogenic densities tdh+ (0.519 log10NMP/g), tdh+/trh+ (0.519 log10NMP/g), and tdh+orf8+ (-0.444 log10NMP/g) were detected on day 3 of storage.
  
  
  CONCLUSIONS
  The results suggest that V. parahaemolyticus growth and pathogenic genes occurrence at 7°C involve changes in the genetic expression as a cold shock response, favoring V. parahaemolyticus survival and virulence, representing a health risk.
authors: Programa de Maestría en Ciencia Animal, Facultad de Medicina, Veterinaria y Zootecnia, Universidad Veracruzana, Jalapa, Veracruz, México.
authors: Argel
authors: A
authors: Flores-Primo
authors: Programa de Maestría en Ciencia Animal, Facultad de Medicina, Veterinaria y Zootecnia, Universidad Veracruzana, Jalapa, Veracruz, México.
authors: Violeta T
authors: VT
authors: Pardío-Sedas
authors: Programa de Maestría en Ciencia Animal, Facultad de Medicina, Veterinaria y Zootecnia, Universidad Veracruzana, Jalapa, Veracruz, México.
authors: Karla
authors: K
authors: López-Hernández
authors: Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, México.
authors: Leonardo
authors: L
authors: Lizárraga-Partida
authors: Programa de Maestría en Ciencia Animal, Facultad de Medicina, Veterinaria y Zootecnia, Universidad Veracruzana, Jalapa, Veracruz, México.
authors: Roxana
authors: R
authors: Uscanga-Serrano
mesh_terms: D000818:Animals; D058491:Bacterial Load; D003080:Cold Temperature; D049896:Crassostrea / Q000382:microbiology*; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D061353:Food Storage / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008452:Maximum Allowable Concentration; D008800:Mexico; D012034:Refrigeration; D012621:Seasons; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence / Q000235:genetics