title: [Effect of Long Non-coding RNA SPRY4-IT1 on Invasion and Migration of A549 Cells].
abstract: OBJECTIVE
  The abnormal expression of human long chain non encoding RNA gene is related to many kinds of tumors. The aim of this study is to investigate the expression of long non-coding RNA maternally expressed gene 3 (SPRY4-IT1) in lung cancer (A549) cells, and to observe the effect of SPRY4-IT1 on the invasion and migration of A549 cells.
  
  
  METHODS
  The levels of SPRY4-IT1 in A549 was detected by Real-time PCR. The effects of SPRY4-IT1 on the invasion and migration of A549 cell were analyzed by MTT and Transwell assay. The expression of matrix metalloproteinase (MMP) family proteins was determined by Western blot.
  
  
  RESULTS
  The invasion and migration of A549 cells were increased after SPRY4-IT1 over-expression. The cell spaces were narrower after SPRY4-IT1 over expression in the wound healing assay. Transwell assays showed that the numbers of transmembrane A549 cells were significantly higher in SPRY4-IT1 over expression group than that in control group (P<0.05). Meanwhile, over expression of SPRY4-IT1 reduced the expression of MMP-2 and MMP-9.
  
  
  CONCLUSIONS
  Over expression of SPRY4-IT1 enhanced the invasion and migration of A549 cells. MMP-2 and MMP-9 might play an important role in this regulation. .
authors: Cancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors: Song
authors: S
authors: Chai
authors: Cancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors: Wenting
authors: W
authors: An
authors: Cancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors: Lili
authors: L
authors: Guo
authors: Cancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors: Le
authors: L
authors: Jiang
authors: Cancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors: Zhisong
authors: Z
authors: Gao
authors: Cancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors: Shujun
authors: S
authors: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000502:physiology
chemical_list: D062085:RNA, Long Noncoding; C000605823:long noncoding RNA SPRY4-IT1, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9