title: Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor.
abstract: In this work, we propose for the first time a sensitive Ochratoxin A (OTA) detection in cocoa beans using competitive aptasensor by differential pulse voltammetry (DPV). In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated. The detection was performed after adding avidin-ALP to perform avidin-biotin reaction; the signal was generated through a suitable substrate 1-naphthyl phosphate (1-NP), for alkaline phosphatase (ALP). The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA. The developed aptasensor showed a good linearity in the range 0.15-5 ng/mL with the limit of detection (LOD) 0.07 ng/mL and 3.7% relative standard deviation (RSD). The aptasensor displayed good recovery values in the range 82.1-85% with 3.87% RSD, thus, demonstrated the efficiency of proposed aptasensor for such matrices.
authors: Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors: Rupesh K
authors: RK
authors: Mishra
authors: Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan; Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors: Akhtar
authors: A
authors: Hayat
authors: Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors: Gaëlle
authors: G
authors: Catanante
authors: Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors: Georges
authors: G
authors: Istamboulie
authors: Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France. Electronic address: jlmarty@univ-perp.fr.
authors: Jean-Louis
authors: JL
authors: Marty
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002099:Cacao / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D059022:Food Safety / Q000379:methods*; D009793:Ochratoxins / Q000737:chemistry*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
keywords: Cocoa beans; Competition based aptasensor; DPV; Food safety; OTA