title: Protective effect of the ω-3 polyunsaturated fatty acids on the schistosomiasis liver fibrosis in mice.
abstract: This study aims to observe the effect of ω-3 polyunsaturated fatty acids on initiation and elimination of the schistosomiasis inflammatory response and liver fibrosis. The mice infected with the cercariae of Schistosoma japonicum (20 ± cercarie per mice) were separated randomly into several groups. After 60 days, liver tissue samples of all mice were sectioned. Hematoxylin-eosin (HE) staining, Masson staining, the enzyme-linked immunosorbent assay (ELISA), and flow cytometry (FCM) were performed. Through HE and Masson staining, the size of egg (ovum) granuloma and the collagen deposited in mice's livers in ω-3 PUFAs and praziquantel mixed groups were less than that of model group and praziquantel treated group. The serum level of IL-13 and TNF-α were lower than that of model group and praziquantel treated group. The indicators of liver fibrosis, such as HA and LN in the group treated with ω-3 PUFAs and praziquantel before the release of soluble eggs antigen (SEA) into blood, were lower than that of model group and praziquantel treated group, respectively. The combined treatment of ω-3 polyunsaturated fatty acids and praziquantel conducted before the release of soluble eggs antigens into the blood decreases liver ovum granulomatous inflammation and fibrosis degree in the schistosomiasis. The mechanism of the ω-3 polyunsaturated fatty acid may be related to the adjustment of the anti-inflammatory and immune responses.
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China ; The Second Affiliated Hospital, University of South China Hengyang 421001, Hunan, China.
authors: Yuan
authors: Y
authors: Wu
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Yu
authors: Y
authors: Liang
authors: The Second Affiliated Hospital, University of South China Hengyang 421001, Hunan, China.
authors: Yi
authors: Y
authors: Zhu
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Yongqiang
authors: Y
authors: Gao
authors: The Second Affiliated Hospital, University of South China Hengyang 421001, Hunan, China.
authors: Hu
authors: H
authors: Chen
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Yukuai
authors: Y
authors: Zhang
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Weiguo
authors: W
authors: Yin
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Yingju
authors: Y
authors: Li
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Kegeng
authors: K
authors: Wang
authors: Institute of Pathogenic Biology of Medical College, University of South China Hengyang 421001, Hunan, China.
authors: Jianhua
authors: J
authors: Xiao
keywords: Schistosoma japonicum; liver fibrosis; protective effect; ω-3 polyunsaturated fatty acids