title: [Study of the Changes on Tree Shrew Bronchial Epithelium  Induced by Xuanwei Bituminous Coal Dust].
abstract: OBJECTIVE
  Lung cancer is the type of cancer with the highest incidence and mortality in numerous countries and regions. Establishing an appropriate animal model that can be used to simulate lung cancer etiology, pathogenesis, and similar processes, is urgent. We explore the feasibility of establishing a lung cancer model induced by Xuanwei bituminous coal dust PM10 (particulate matter with diameters of 10 μm or less), which affects bronchial epithelium of tree shrews.
  
  
  METHODS
  The neck skin of adult tree shrews is dissected, and the thyroid cartilage is fully exposed. Subsequently, the weak part at the top of the thyroid cartilage is treated with intratracheal agents by perfusion via a special infusion needle puncture method. Regular X-ray examination and lung tissue biopsy were performed on the sacrificed animals to observe changes in pulmonary imaging and bronchial epithelial cells after perfusion of Xuanwei bituminous coal dust PM 10.
  
  
  RESULTS
  The tree shrews of the experimental group (exposed to bituminous coal dust) died in a week after perfusion with PM10, whereas no animal died until the end of the experiment in the blank control and the solvent control groups. Sections of lung tissue biopsy of the regularly killed tree shrews were stained with hematoxylin and eosin. The lung tissues of tree shrews in the experimental group showed a serial changes caused by bronchial epithelial hyperplasia, such as squamous metaplasia, dysplasia, and early invasive carcinoma, whereas no significant pathological changes were observed in the blank control and solvent control groups.
  
  
  CONCLUSIONS
  Endotracheal infusion of Xuanwei bituminous coal dust PM10 induces lung cancer in tree shrews. Thus, the lung cancer model was established. .
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Xiaobo
authors: X
authors: Chen
authors: Department of Cardiothoracic Surgery, Rizhao People's Hospital, Rizhao 276800, China.
authors: Meng
authors: M
authors: He
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Guangjian
authors: G
authors: Li
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Yongchun
authors: Y
authors: Zhou
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Guangqiang
authors: G
authors: Zhao
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Yujie
authors: Y
authors: Lei
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Kaiyun
authors: K
authors: Yang
authors: Shenzhen Research Institute of Chinese University Hong Kong, Shenzhen 518057, China.
authors: Linwei
authors: L
authors: Tian
authors: Department of Cardiothoracic Surgery, the Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, China.
authors: Yunchao
authors: Y
authors: Huang
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D000066428:Coal Industry; D004785:Environmental Pollutants / Q000633:toxicity*; D004848:Epithelium / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced / Q000473:pathology; D020545:Respiratory Mucosa / Q000187:drug effects / Q000473:pathology; D014418:Tupaiidae
chemical_list: D004785:Environmental Pollutants