title: Clonally Related Forebrain Interneurons Disperse Broadly across Both Functional Areas and Structural Boundaries.
abstract: The medial ganglionic eminence (MGE) gives rise to the majority of mouse forebrain interneurons. Here, we examine the lineage relationship among MGE-derived interneurons using a replication-defective retroviral library containing a highly diverse set of DNA barcodes. Recovering the barcodes from the mature progeny of infected progenitor cells enabled us to unambiguously determine their respective lineal relationship. We found that clonal dispersion occurs across large areas of the brain and is not restricted by anatomical divisions. As such, sibling interneurons can populate the cortex, hippocampus striatum, and globus pallidus. The majority of interneurons appeared to be generated from asymmetric divisions of MGE progenitor cells, followed by symmetric divisions within the subventricular zone. Altogether, our findings uncover that lineage relationships do not appear to determine interneuron allocation to particular regions. As such, it is likely that clonally related interneurons have considerable flexibility as to the particular forebrain circuits to which they can contribute.
authors: NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA.
authors: Christian
authors: C
authors: Mayer
authors: NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA.
authors: Xavier H
authors: XH
authors: Jaglin
authors: NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA.
authors: Lucy V
authors: LV
authors: Cobbs
authors: NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA.
authors: Rachel C
authors: RC
authors: Bandler
authors: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors: Carmen
authors: C
authors: Streicher
authors: Departments of Genetics and Ophthalmology and HHMI, Harvard Medical School, Boston, MA 02115, USA.
authors: Constance L
authors: CL
authors: Cepko
authors: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors: Simon
authors: S
authors: Hippenmeyer
authors: NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA. Electronic address: fisheg01@nyumc.org.
authors: Gord
authors: G
authors: Fishell
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D058893:DNA Barcoding, Taxonomic; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015723:Gene Library; D005829:Geniculate Bodies / Q000166:cytology* / Q000196:embryology; D007395:Interneurons / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D042282:Microdissection; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics; D016548:Prosencephalon / Q000166:cytology* / Q000196:embryology; D013234:Stem Cells / Q000502:physiology*; D000074482:Thyroid Nuclear Factor 1; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; C486015:Ndel1 protein, mouse; C577485:Nes protein, mouse; D064231:Nestin; D009687:Nuclear Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C513190:Pafah1b1 protein, mouse