title: B subunits of cholera toxin and thermolabile enterotoxin of Escherichia coli have similar adjuvant effect as whole molecules on rotavirus 2/6-VLP specific antibody responses and induce a Th17-like response after intrarectal immunization.
abstract: The purpose of this study was to evaluate the adjuvant effect of the B subunits of cholera toxin (CT) and the thermolabile enterotoxin of Escherichia coli (LT) by the intrarectal route of immunization and compare them to the whole molecules CT and LT-R192G, a non toxic mutant of LT, using 2/6-VLP as an antigen, in mice. All molecules induced similar antigen specific antibody titers in serum and feces, whereas different T cell profiles were observed. CTB and LTB, conversely to CT and LT-R192G, did not induce detectable production of IL-2 by antigen specific T cells. Moreover, CTB, conversely to LT-R192G, CT and LTB, did not induce antigen specific CD4+CD25+Foxp3- and Foxp3+ T cells, thus showing different effects between the B subunits themselves. However, all molecules induced an antigen specific Th17 response. In conclusion, B subunits are potent adjuvants on B cell responses by the intrarectal route. Although their impact on T cell responses are different, all molecules induce a 2/6-VLP-specific Th17 T cell response that may play a major role in helping B cell responses and thus in adjuvanticity and protection.
authors: Centre de Recherche Inserm UMR866 'Lipides, Nutrition, Cancer', Université de Bourgogne Franche-Comté, Dijon, France.
authors: Fatou
authors: F
authors: Thiam
authors: Virologie Moléculaire et Structurale, UMR CNRS 2472 INRA 1157, Gif/Yvette, France.
authors: Annie
authors: A
authors: Charpilienne
authors: Virologie Moléculaire et Structurale, UMR CNRS 2472 INRA 1157, Gif/Yvette, France.
authors: Didier
authors: D
authors: Poncet
authors: Centre de Recherche Inserm UMR866 'Lipides, Nutrition, Cancer', Université de Bourgogne Franche-Comté, Dijon, France.
authors: Evelyne
authors: E
authors: Kohli
authors: Centre de Recherche Inserm UMR866 'Lipides, Nutrition, Cancer', Université de Bourgogne Franche-Comté, Dijon, France. Electronic address: christelle.basset@u-bourgogne.fr.
authors: Christelle
authors: C
authors: Basset
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000285:Administration, Rectal; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood*; D000917:Antibody Formation; D001427:Bacterial Toxins / Q000008:administration & dosage*; D002772:Cholera Toxin / Q000008:administration & dosage*; D004768:Enterotoxins / Q000008:administration & dosage*; D029968:Escherichia coli Proteins / Q000008:administration & dosage*; D005243:Feces / Q000737:chemistry; D007156:Immunologic Memory; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007376:Interleukin-2; D022243:Rotavirus Vaccines; D058425:Vaccines, Virus-Like Particle; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli
keywords: B subunit; Cholera toxin; Immunization; Intrarectal; LT-R192G; Rotavirus