title: Flow Cytometry Analysis of Thymic Epithelial Cells and Their Subpopulations.
abstract: The parenchyma of the thymus is compartmentalized into the cortex and the medulla, which are constructed by cortical thymic epithelial cells (cortical TECs, cTECs) and medullary thymic epithelial cells (mTECs), respectively. cTECs and mTECs essentially and differentially regulate the development and repertoire selection of T cells. Consequently, the biology of T cell development and selection includes the study of TECs in addition to the study of developing T cells and other hematopoietic cells including dendritic cells. In this chapter, we describe the methods for flow cytometric analysis and sorting of TECs and their subpopulations, including cTECs and mTECs.
authors: Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan.
authors: Izumi
authors: I
authors: Ohigashi
authors: Yousuke
authors: Y
authors: Takahama
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002469:Cell Separation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D016130:Immunophenotyping / Q000379:methods; D051379:Mice; D013194:Staining and Labeling / Q000379:methods; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D015415:Biomarkers