title: Long-term effect of pH on short-chain fatty acids accumulation and microbial community in sludge fermentation systems.
abstract: Long-term effect of pH (4, 10, and uncontrolled) on short-chain fatty acid (SCFA) accumulation, microbial community and sludge reduction were investigated in waste activated sludge (WAS) fermentors for over 90days. The average SCFAs accumulation was 1721.4 (at pH 10), 114.2 (at pH 4), and 58.1 (at uncontrolled pH) mg chemical oxygen demand (COD)/L. About 31.65mgCOD/L was produced at pH 10, accounting for 20% of the influent COD. Illumina MiSeq sequencing revealed that Alcaligenes (hydrolic bacteria) and Erysipelothrix (acidogenic bacteria) were enriched at pH 10, while less acidogenic bacteria existed at pH 4 than pH 10, and no acidogenic bacteria were detected at the uncontrolled pH. The ratios of archaea to bacteria were 1:41, 1:16, and 1:9 at the pH of 10, 4, and uncontrolled. This study elucidated the effects of pH on WAS fermentation, and established the correlation of microbial structure with SCFAs accumulations and sludge reduction.
authors: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors: Yue
authors: Y
authors: Yuan
authors: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors: Shuying
authors: S
authors: Wang
authors: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors: Ye
authors: Y
authors: Liu
authors: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China; Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT 06269, USA.
authors: Baikun
authors: B
authors: Li
authors: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors: Bo
authors: B
authors: Wang
authors: Engineering Research Center of Beijing, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, PR China. Electronic address: pyz@bjut.edu.cn.
authors: Yongzhen
authors: Y
authors: Peng
mesh_terms: D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D059013:Microbial Consortia; D012722:Sewage / Q000382:microbiology*
chemical_list: D005232:Fatty Acids, Volatile; D012722:Sewage
keywords: Microbial community; Short-chain fatty acids (SCFAs); Sludge fermentation; Sludge reduction; pH