title: Camelimonas fluminis sp. nov., a cyhalothrin-degrading bacterium isolated from river water.
abstract: An aerobic, Gram-stain-negative, short rod-shaped, non-spore-forming, cyhalothrin-degrading bacterial strain, XZ2(T), was isolated from the surface water of Hanjiang River in Wuhan, China. Strain XZ2(T) grew optimally at pH 6.0, 30 °C and in the absence of NaCl. The G+C content of the total DNA was 64.1 mol%. The 16S rRNA gene sequence of strain XZ2(T) showed the highest similarity to that of Camelimonas lactis M 2040(T) (99.1%), followed by Camelimonas abortus UK34/07-5(T) (95.9%) and Chelatococcus daeguensis K106(T) (95.3%). The major cellular fatty acids of strain XZ2(T) were C19 : 0 cyclo ω8c (63.1%), C16 : 0 (15.0%) and C18 : 1ω7c/C18 : 1ω6c (summed feature 8; 8.9%). C18 : 0 3-OH was also detected as the major hydroxylated fatty acid. The respiratory quinone was ubiquinone Q-10. The polar lipid profile included the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylglycerol and two unidentified aminolipids. The predominant compound in the polyamine pattern was spermidine. These chemotaxonomic data supported the affiliation of strain XZ2(T) to the genus Camelimonas. The DNA-DNA hybridization value between strain XZ2(T) and Camelimonas lactis M 2040(T) was 43.5 ± 0.6%. DNA-DNA hybridization data as well as biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiations between strain XZ2(T) and Camelimonas lactis M 2040(T). Therefore, strain XZ2(T) represents a novel species of the genus Camelimonas, for which the name Camelimonas fluminis sp. nov. is proposed. The type strain is XZ2(T) ( = KCTC 42282(T) = ACCC 19738(T)).
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Long
authors: L
authors: Zhang
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Man
authors: M
authors: Song
authors: China National Center for Biotechnology Development, Building 4, No. 16, Xisihuanzhonglu, Haidian District, 100039, Beijing, PR China.
authors: Qin
authors: Q
authors: Cao
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Shang
authors: S
authors: Wu
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Yan
authors: Y
authors: Zhao
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Jun-Wei
authors: JW
authors: Huang
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Kai
authors: K
authors: Chen
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Shun-Peng
authors: SP
authors: Li
authors: Yunnan Academy of Tobacco Agricultural Science, 650031, Kunming, PR China.
authors: Zhen-Yuan
authors: ZY
authors: Xia
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
authors: Jian-Dong
authors: JD
authors: Jiang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042062:Beijerinckiaceae / Q000145:classification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009570:Nitriles; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011722:Pyrethrins; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000737:chemistry; D014451:Ubiquinone
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009570:Nitriles; D010743:Phospholipids; D011722:Pyrethrins; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine; C037304:cyhalothrin