title: Effects of Dietary Genistein on Plasma and Liver Lipids, Hepatic Gene Expression, and Plasma Metabolic Profiles of Hamsters with Diet-Induced Hyperlipidemia.
abstract: Male hamsters were fed one of the following three diets for 6 weeks (n = 15): normal-fat diet (NFD), high-fat diet (HFD), or HFD + 2 g/kg genistein; the effects of dietary genistein on hyperlipidemia were investigated using traditional and (1)H NMR metabonomic approaches. At 6 weeks, compared with the hamsters in the NFD group, those in the HFD group had higher plasma and liver lipids (P < 0.05). Hyperlipidemia was alleviated in the genistein group, with lower plasma cholesterol (9.11 ± 0.40 vs 12.4 ± 0.37 mmol/L), triglyceride (8.07 ± 1.08 vs 14.7 ± 1.18 mmol/L), LDL cholesterol (2.69 ± 0.20 vs 4.48 ± 0.27 mmol/L), malondialdehyde (7.77 ± 1.64 vs 14.0 ± 1.15 μmol/L), and liver cholesterol (20.9 ± 1.01 vs 29.9 ± 2.76 μmol/g) than those in the HFD group (P < 0.05). Expression of hepatic LDL receptor and estrogen receptors α and β mRNA in the genistein group were significantly up-regulated, compared with those of the HFD group (P < 0.05). In the (1)H NMR metabonomic analysis, both the small and macromolecular plasma metabolite profiles differed among the three groups, and the metabolic profile of the genistein group was shifted toward that of the NFD group. These results extend our understanding of the beneficial effects of genistein on hyperlipidemia.
authors: Institute of Animal Science, Chinese Academy of Agricultural Sciences , No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, China.
authors: Chaohua
authors: C
authors: Tang
authors: Institute of Animal Science, Chinese Academy of Agricultural Sciences , No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, China.
authors: Kai
authors: K
authors: Zhang
authors: Institute of Animal Science, Chinese Academy of Agricultural Sciences , No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, China.
authors: Qingyu
authors: Q
authors: Zhao
authors: Institute of Animal Science, Chinese Academy of Agricultural Sciences , No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, China.
authors: Junmin
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D006224:Cricetinae; D059305:Diet, High-Fat / Q000009:adverse effects; D015870:Gene Expression; D019833:Genistein / Q000378:metabolism*; D006801:Humans; D006949:Hyperlipidemias / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D011960:Receptors, Estrogen; D011973:Receptors, LDL; D014280:Triglycerides; D002784:Cholesterol; D019833:Genistein
keywords: genistein; hamster; hepatic gene expression; hyperlipidemia; liver lipid; plasma lipid; plasma metabolic profile