title: Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran.
abstract: BACKGROUND
  The aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates.
  
  
  METHODS
  Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The bla(CTX-M) cluster was identified using the RFLP method, and its genotype was sequenced.
  
  
  RESULTS
  From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The bla(CTX-M) gene was identical to CTX-M-15.
  
  
  CONCLUSIONS
  A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.
authors: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
authors: Khosrow
authors: K
authors: Zamani
authors: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
authors: Amir
authors: A
authors: Emami
authors: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
authors: Abdollah
authors: A
authors: Bazargani
authors: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
authors: Afagh
authors: A
authors: Moattari
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D052978:Disk Diffusion Antimicrobial Tests; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007492:Iran; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases