title: Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.
abstract: A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
authors: Birgit
authors: B
authors: Weyand
authors: Biomimetics Technologies Inc., 191 Ellis Avenue, Toronto, ON, Canada M6S 2X4.
authors: Meir
authors: M
authors: Israelowitz
authors: 8 Selden Street, Newton, MA 02468, USA.
authors: James
authors: J
authors: Kramer
authors: Habsburger Allee 56 B, 76767 Hagenbach, Germany.
authors: Christian
authors: C
authors: Bodmer
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
authors: Mariel
authors: M
authors: Noehre
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
authors: Sarah
authors: S
authors: Strauss
authors: Colibri Photonics GmbH, Am Mühlenberg 11, 14476 Potsdam, Germany.
authors: Elmar
authors: E
authors: Schmälzlin
authors: Biomimetics Technologies Inc., 191 Ellis Avenue, Toronto, ON, Canada M6S 2X4.
authors: Christoph
authors: C
authors: Gille
authors: Biomimetics Technologies Inc., 191 Ellis Avenue, Toronto, ON, Canada M6S 2X4 ; Toronto Western Hospital, University of Toronto, East Wing 2nd Floor, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8.
authors: Herbert P
authors: HP
authors: von Schroeder
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
authors: Kerstin
authors: K
authors: Reimers
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
authors: Peter M
authors: PM
authors: Vogt
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003198:Computer Simulation; D006801:Humans; D057446:Hydrodynamics; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts / Q000166:cytology; D010100:Oxygen / Q000378:metabolism*; D016062:Porosity; D011312:Pressure; D054457:Tissue Scaffolds
chemical_list: D010100:Oxygen