title: Spatio-temporal Variation of Sediment Methanotrophic Microorganisms in a Large Eutrophic Lake.
abstract: Aerobic methane-oxidizing bacteria (MOB) play a crucial role in mitigating the methane emission from lake ecosystems to the atmosphere. However, the distribution of methanotrophic community in shallow and eutrophic lake and its influential factors remain essentially unclear. The present study investigated sediment methanotrophic microorganisms at different sites in eutrophic freshwater Dianchi Lake (China) in two different seasons. The abundance, diversity, and structure of sediment methanotrophic community showed a profound spatial and seasonal variation. The pmoA gene copy number in lake sediments ranged from 8.71 ± 0.49 × 10(4) to 2.09 ± 0.03 × 10(7) copies per gram of dry sediment. Sediment methanotrophic communities were composed of Methylococcus and Methylobacter (type I methanotrophs) and Methylosinus (type II methanotrophs), while type I MOB usually outnumbered type II MOB. Moreover, ammonia nitrogen was found to be a potential determinant of methanotrophic community structure in Dianchi Lake.
authors: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
authors: Yuyin
authors: Y
authors: Yang
authors: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
authors: Qun
authors: Q
authors: Zhao
authors: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
authors: Yahui
authors: Y
authors: Cui
authors: Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
authors: Yilin
authors: Y
authors: Wang
authors: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China. xiesg@pku.edu.cn.
authors: Shuguang
authors: S
authors: Xie
authors: Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China. yongliu@pku.edu.cn.
authors: Yong
authors: Y
authors: Liu
mesh_terms: D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D044822:Biodiversity; D005068:Eutrophication; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology; D060106:Lakes / Q000737:chemistry / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012621:Seasons
chemical_list: D001426:Bacterial Proteins; D008697:Methane
keywords: Environmental factors; Freshwater sediment; Methane oxidization; Methanotrophs; Microbial community