title: Construction of an oral vaccine for transmissible gastroenteritis virus based on the TGEV N gene expressed in an attenuated Salmonella typhimurium vector.
abstract: This research aimed to develop an oral vaccine for transmissible gastroenteritis virus (TGEV) based on the TGEV N gene expressed in an attenuated Salmonella typhimurium vector and aimed to evaluate the vaccine's immune response in piglets. Recombinant plasmid pVAX-N was transformed into competent cells of attenuated S. typhimurium SL7207 via electroporation. After it was identified via RT-PCR and double digestion, the screened recombinant bacteria presenting pVAX-N were named SL7207 (pVAX-N). To evaluate the safety and stability of the developed vaccine, different dosages (5 × 10(8), 1 × 10(9), and 2 × 10(9) CFU/mice) of SL7207 (pVAX-N) were inoculated to 6-week-old mice. Piglets below 20 days of age were dosed with 1 × 10(12) CFU. Humoral (neutralization titer and specific IgG), cellular (interleukin-4, γ-interferon, and peripheral lymphocyte proliferation), and mucosal (sIgA) immune responses were detected and evaluated. The three immunizing dosages were determined to be safe for mice and were completely eliminated 8 weeks after the first inoculation. Results of antibody and cytokine detection indicated that SL7207 (pVAX-N) could significantly induce antibody-IgG, antibody-IgA, interleukin-4, and γ-interferon, whose value was maximized on the 6th week. Results confirmed that the recombinant vaccine increased the proliferation of peripheral T lymphocyte. In conclusion, the oral vaccine was developed successfully, and the vaccine could significantly induce humoral, cellular, and mucosal immune responses in piglets.
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China.
authors: Dan
authors: D
authors: Zhang
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Biological Technology, Ministry of Agriculture, Ya'an 625014, China. Electronic address: juanlian7686@sina.com.
authors: Xiaobo
authors: X
authors: Huang
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China.
authors: Xiaohui
authors: X
authors: Zhang
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Biological Technology, Ministry of Agriculture, Ya'an 625014, China.
authors: Sanjie
authors: S
authors: Cao
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Biological Technology, Ministry of Agriculture, Ya'an 625014, China.
authors: Xintian
authors: X
authors: Wen
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Biological Technology, Ministry of Agriculture, Ya'an 625014, China.
authors: Yiping
authors: Y
authors: Wen
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Biological Technology, Ministry of Agriculture, Ya'an 625014, China.
authors: Rui
authors: R
authors: Wu
authors: Laboratory of Animal Infectious Disease and Microarray, Laboratory of Zoonosis and Pig Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China.
authors: Entao
authors: E
authors: Liang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005814:Genes, Viral; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids; D012486:Salmonella typhimurium; D034421:Sus scrofa; D005760:Transmissible gastroenteritis virus / Q000235:genetics / Q000276:immunology*; D064166:Vaccine Potency; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Attenuated Salmonella typhimurium; Immunogenicity; N gene; Oral vaccine; Transmissible gastroenteritis virus