title: Overexpression of SPAG9 in human gastric cancer is correlated with poor prognosis.
abstract: Sperm associated antigen 9 (SPAG9) protein has been found to play an important role in cancer progression but the involved mechanisms are still obscure. Its clinical significance in human gastric cancers remains unexplored. In the present study, SPAG9 expression was analyzed in 147 gastric cancer specimens. We observed weak staining in normal gastric mucosa and positive staining in 65 out of 147 (44.2 %) cancer samples. Overexpression of SPAG9 correlated with local invasion (p = 0.0101), lymph node metastasis (p = 0.0488), TNM stage (p = 0.0002), and relapse (p = 0.0018). Importantly, SPAG9 overexpression correlated with poor overall survival (p = 0.0008). Furthermore, we performed siRNA knockdown of SPAG9 in HGC-27 cells with high endogenous expression and transfected SPAG9 plasmid in SGC-7901 cell line with low endogenous level. SPAG9 overexpression promoted while its depletion inhibited cell proliferation, cell cycle transition, and invasive cell growth. SPAG9 overxpression also increased chemoresistance to 5--fluorouracil (5-FU) in SGC-7901 cells. Further analysis showed that SPAG9 knockdown downregulated and its overexpression upregulated cyclin D1, MMP9, and p-p38 expression. In conclusion, SPAG9 overexpression in gastric cancer correlates with poor prognosis and contributes to gastric cancer cell proliferation, invasion, and chemoresistance. SPAG9 promotes gastric cancer invasion, possibly through p38-MMP9 signaling pathways.
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China.
authors: Zhi-Feng
authors: ZF
authors: Miao
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China.
authors: Zhen-Ning
authors: ZN
authors: Wang
authors: Department of Breast Surgery, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors: Ting-Ting
authors: TT
authors: Zhao
authors: Department of Breast Surgery, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors: Ying-Ying
authors: YY
authors: Xu
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China.
authors: Jian-Hua
authors: JH
authors: Wu
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China.
authors: Xing-Yu
authors: XY
authors: Liu
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China.
authors: Hao
authors: H
authors: Xu
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China.
authors: Yi
authors: Y
authors: You
authors: Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, No. 155 North Nanjing Street, Heping District, Shenyang, Liaoning, 110001, China. xuhuimpaper@163.com.
authors: Hui-Mian
authors: HM
authors: Xu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008207:Lymphatic Metastasis / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C111047:SPAG9 protein, human
keywords: Chemoresistance; Gastric cancer; Proliferation; SPAG9