title: Determination of nitrofurans metabolites residues in aquatic products by ultra-performance liquid chromatography-tandem mass spectrometry.
abstract: A method was described for monitoring four nitrofuran metabolites including 5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ), 3-amino-2-oxazolidone (AOZ), 1-amino-hydantoin (AHD) and semicarbazide (SEM) in aquatic products. The analytes were quantified by ultra-performance liquid chromatography-tandem mass spectrometry operating in positive ion multiple monitoring mode (MRM) after 2-nitrobenzaldehyde derivatization. The matrix calibration curve was established with correlation coefficient (R(2)>0.99) in the range of 1-100 ng ml(-1). Limit of detection and limit of quantification for all analytes were 0.5 and 1.5 μg kg(-1), respectively. Recovery rates and relative standard deviations ranged from 88% to 112% and 2% to 4%, respectively. The validated method was successfully applied to detect nitrofuran metabolites in 120 fish samples. The analytes were detected in 6/16 species of fish samples, with a total detection rate of 6.5%. AOZ was most frequently detected (8.3%), followed by AMOZ (7.5%), AHD (5.0%) and SEM (5.0%). The method is proposed for monitoring nitrofuran metabolites in aquatic products.
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: zhangyibei0666@163.com.
authors: Yibei
authors: Y
authors: Zhang
authors: Shaanxi Provincial Center for Disease Control and Prevention, Xi'an, Shaanxi 710054, PR China.
authors: Haiou
authors: H
authors: Qiao
authors: School and Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia.
authors: Chen
authors: C
authors: Chen
authors: Department of Public Health, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, PR China.
authors: Zhilun
authors: Z
authors: Wang
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: foodscixiaodong@yahoo.com.
authors: Xiaodong
authors: X
authors: Xia
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D009581:Nitrofurans / Q000032:analysis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009581:Nitrofurans
keywords: Aquatic products; Nitrofuran metabolites; Residue determination; Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS)