title: Development and Implementation of Autoverification Rules for ELISA Results of HBV Serological Markers.
abstract: Autoverification is a process of using computer-based rules to verify clinical laboratory test results without manual review. But to date, there are few published articles on the use of autoverification over the course of years in a clinical laboratory. In our study, we firstly described the development and implementation of autoverification rules for enzyme-linked immunosorbent assay (ELISA) results of hepatitis B virus (HBV) serological markers in a clinical immunology laboratory. We designed the autoverification rules for HBV by using Boolean logic on five clinically used serological markers in accordance with the framework of AUTO-10A, issued by the American Clinical Laboratory Standards Institute in 2006. The rules were written into the laboratory information system (LIS) and installed in the computer, so we could use the LIS to screen the test results. If the results passed the autoverification rules, they could be sent to doctors immediately. To evaluate the autoverification rules, we applied the real-time data of 11,585 patients with the autoverification rules. The autoverification rate of the five HBV serological markers was 79.5%. Furthermore, the turnaround time (TAT) was reduced by 38% (78 minutes vs. 126 minutes). The error rate was nearly eliminated. These results show that using LIS with autoverification rules can shorten TAT, enhance efficiency, and reduce manual review errors.
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Jiancheng
authors: J
authors: Li
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Bizhen
authors: B
authors: Cheng
authors: Department of Clinical Laboratory, Shantou Central Hospital, Guangdong, People's Republic of China.
authors: Li
authors: L
authors: Yang
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Ying
authors: Y
authors: Zhao
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Meichen
authors: M
authors: Pan
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Gaozhe
authors: G
authors: Zheng
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Xiaoyan
authors: X
authors: Xu
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Jing
authors: J
authors: Hu
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China.
authors: Tongtong
authors: T
authors: Xiao
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Guangdong, People's Republic of China st_ymcai@126.com.
authors: Yingmu
authors: Y
authors: Cai
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D015415:Biomarkers / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000175:diagnosis*; D006801:Humans; D012698:Serologic Tests / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: autoverification; delta check; hepatitis B serological marker; laboratory information system; turnaround time