title: Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression.
abstract: BACKGROUND
  Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells. Nevertheless, few in vivo experiments have provided data of its ability on apoptosis protein expression in tumor tissue. Thus, in this study we investigate the effect of FO supplementation on apoptosis protein expression in Walker 256 tumor bearing rats. Male Wistar rats were randomly assigned to three groups: fed with regular chow (W); fed regular chow supplemented with FO (WFO) or coconut fat (WCO) (1 g/kg body weight/daily). After thirty days, all animals were inoculated subcutaneously with Walker 256 tumor cells.
  
  
  RESULTS
  Protein expression was done by western blotting in Walker 256 tumor tissue samples. FO decreased the Bcl-2/Bax ratio (p < 0.05) and increased the p53 (p < 0.05), cleaved caspase-7 (p < 0.05) and cleaved caspase-3 (p < 0.05) in Walker 256 tumor tissue.
  
  
  CONCLUSIONS
  Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.
authors: Centro de Estudos da Biodiversidade, Universidade Federal de Roraima, Campus Paricarana Avenida Capitão Ene Garcez, 2413, Bairro Aeroporto Cep: 69310-000, Boa Vista, Roraima, Brazil. borghettigina@hotmail.com.
authors: Gina
authors: G
authors: Borghetti
authors: Instituto de Pesquisa Pelé Pequeno Príncipe, Hospital Pequeno Príncipe, Curitiba, Paraná, Brasil. yamaguchi.dri@gmail.com.
authors: Adriana Aya
authors: AA
authors: Yamaguchi
authors: Departamento de Fisiologia, Laboratório de Metabolismo Celular, Universidade Federal do Paraná, Curitiba, Paraná, Brasil. juliaaikawa@gmail.com.
authors: Julia
authors: J
authors: Aikawa
authors: Universidade Federal da Fronteira Sul, Laranjeiras do Sul, Paraná, Brasil. rkyamazaki@gmail.com.
authors: Ricardo Key
authors: RK
authors: Yamazaki
authors: Universidade Federal da Integração Latina Americana, Foz do Iguaçu, Parana, Brazil. gapbrito@yahoo.com.br.
authors: Gleisson Alisson Pereira
authors: GA
authors: de Brito
authors: Departamento de Fisiologia, Laboratório de Metabolismo Celular, Universidade Federal do Paraná, Curitiba, Paraná, Brasil. lcfer@ufpr.br.
authors: Luiz Claudio
authors: LC
authors: Fernandes
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002279:Carcinoma 256, Walker / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D000074263:Coconut Oil; D019587:Dietary Supplements; D005395:Fish Oils / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D007279:Injections, Subcutaneous; D008297:Male; D010938:Plant Oils / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D047368:Tumor Burden / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D016588:Anticarcinogenic Agents; D005395:Fish Oils; D010938:Plant Oils; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3; D053179:Caspase 7; D000074263:Coconut Oil