title: AGXT2L1 is down-regulated in heptocellular carcinoma and associated with abnormal lipogenesis.
abstract: OBJECTIVE
  To clarify the clinical implications and functional role of the alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1) gene in hepatocellular carcinoma (HCC).
  
  
  RESULTS
  We confirmed that AGXT2L1 was down-regulated in liver cancer samples by immunohistochemical (IHC) staining. We also demonstrated that this down-regulation was associated with several clinicopathological features such as alpha fetoprotein (AFP) serum level and T stage. Furthermore, we showed with Kaplan-Meier analysis that expression of AGXT2L1 in tumour samples was significantly correlated with patient prognosis. The bioinformatic tool indicated that AGXT2L1 plays a role in the lipid metabolic process of HCC tissue, while siRNA silenced the expression of AGXT2L1 in HCC 97H and LM3 cells, confirming that down-regulation of AGXT2L1 promotes the lipogenesis of cancer cells.
  
  
  CONCLUSIONS
  For the first time, we have shown that AGXT2L1 is down-regulated in HCC and its low expression indicates a poor prognosis. Our findings also demonstrated that AGXT2L1 is a crucial gene in the abnormal lipogenesis of HCC tissue.
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Qianshan
authors: Q
authors: Ding
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Jian
authors: J
authors: Kang
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Jinfen
authors: J
authors: Dai
authors: Department of Immunology, School of Basic Medicine, Wuhan University, Wuhan, China.
authors: Meng
authors: M
authors: Tang
authors: Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Qi
authors: Q
authors: Wang
authors: Department of Math and Statistics, Liberal Arts College, Portland State University, Portland, OR, USA.
authors: Haotian
authors: H
authors: Zhang
authors: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Wenyi
authors: W
authors: Guo
authors: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Rongze
authors: R
authors: Sun
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.
authors: Honggang
authors: H
authors: Yu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D019295:Computational Biology; D030541:Databases, Genetic; D015536:Down-Regulation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D050155:Lipogenesis; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D016896:Treatment Outcome; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: C097353:AFP protein, human; D000509:alpha-Fetoproteins; D000637:Transaminases; C019635:Alanine-glyoxylate transaminase
keywords: CANCER RESEARCH; LIPIDS; LIVER CANCER; TUMOUR MARKERS