title: CCDC34 is up-regulated in bladder cancer and regulates bladder cancer cell proliferation, apoptosis and migration.
abstract: The coiled coil is a superhelical structural protein motif involved in a diverse array of biological functions, and the abnormal expression of the coiled-coil domain containing proteins has a direct link with the phenotype of tumor cell migration, invasion and metastasis. The aim of this study was to investigate the critical role of Coiled-coil domain-containing protein 34 (CCDC34) in bladder carcinogenesis, which has never been reported to date. Here, we found CCDC34 expression was elevated in bladder cancer tissues and cell lines. The knockdown of CCDC34 via lentivirus-mediated siRNA significantly suppressed bladder cancer cells proliferation and migration, and induced cell cycle arrest at G2/M phase and increased apoptosis in vitro. In addition, CCDC34 knockdown suppressed bladder tumor growth in nude mice. Moreover, CCDC34 silencing decreased the phosphorylation of MEK, ERK1/2, JNK, p38 and Akt, and the expressions of c-Raf and c-Jun, indicating MAPK and AKT pathways (ERK/MAPK, p38/MAPK, JNK/MAPK and PI3K/Akt) might be involved in CCDC34 regulation of bladder cancer cell proliferation and migration. Our findings revealed for the first time a potential oncogenic role for CCDC34 in bladder carcinoma pathogenesis and it may serve as a biomarker or even a therapeutic target for bladder cancer.
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.|National Urological Cancer Center, Beijing 100034, China.
authors: Yanqing
authors: Y
authors: Gong
authors: Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors: Wei
authors: W
authors: Qiu
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.
authors: Xianghui
authors: X
authors: Ning
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.|National Urological Cancer Center, Beijing 100034, China.
authors: Xinyu
authors: X
authors: Yang
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.|National Urological Cancer Center, Beijing 100034, China.
authors: Libo
authors: L
authors: Liu
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.
authors: Zicheng
authors: Z
authors: Wang
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.|National Urological Cancer Center, Beijing 100034, China.
authors: Jian
authors: J
authors: Lin
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.|National Urological Cancer Center, Beijing 100034, China.
authors: Xuesong
authors: X
authors: Li
authors: Department of Urology, Peking University First Hospital, Beijing 100034, China.|Institute of Urology, Peking University, Beijing 100034, China.|National Urological Cancer Center, Beijing 100034, China.
authors: Yinglu
authors: Y
authors: Guo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004789:Enzyme Activation; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D000067492:RNAi Therapeutics; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D023041:Xenograft Model Antitumor Assays; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; C000609204:CCDC34 protein, human; D009363:Neoplasm Proteins; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases
keywords: CCDC34; bladder cancer; migration; proliferation; siRNA