title: Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis.
abstract: Cecropin B (CB) is a very effective natural antimicrobial peptide that has shown great potential for future antimicrobial drug development. HAPS_2096 is a Haemophilus parasuis gene that encodes the periplasmic substrate-binding protein of an ATP-binding cassette-type amino acid transporter. In this research, we constructed and verified an HAPS_2096 deletion mutant and a complementary HAPS_2096 mutant of H. parasuis JS0135. A bactericidal assay revealed that the HAPS_2096 deletion mutant was significantly more sensitive than the wild-type strain to 0.25-0.5 µg/ml CB. However, the gene complementation alleviated the CB sensitivity of the mutant. Immunoelectron microscopy observation following a 30-min treatment with a sublethal concentration of CB (0.25 μg/ml) revealed more extensive morphological damage in the mutant strain than in the wild-type strain. Hence, our results suggest that the HAPS_2096 gene contributes to H. parasuis resistance to CB.
authors: Division of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Wuhan, PR China.
authors: Fanjie
authors: F
authors: Chen
authors: Han
authors: H
authors: Hu
authors: Zhonghua
authors: Z
authors: Li
authors: Jiacheng
authors: J
authors: Huang
authors: Xuwang
authors: X
authors: Cai
authors: Chunmei
authors: C
authors: Wang
authors: Qigai
authors: Q
authors: He
authors: Jiyue
authors: J
authors: Cao
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D054807:Cecropins / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D017353:Gene Deletion; D044137:Haemophilus parasuis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D054807:Cecropins