title: The Mitochondrial Genomes of Aquila fasciata and Buteo lagopus (Aves, Accipitriformes): Sequence, Structure and Phylogenetic Analyses.
abstract: The family Accipitridae is one of the largest groups of non-passerine birds, including 68 genera and 243 species globally distributed. In the present study, we determined the complete mitochondrial sequences of two species of accipitrid, namely Aquila fasciata and Buteo lagopus, and conducted a comparative mitogenome analysis across the family. The mitogenome length of A. fasciata and B. lagopus are 18,513 and 18,559 bp with an A + T content of 54.2% and 55.0%, respectively. For both the two accipitrid birds mtDNAs, obvious positive AT-skew and negative GC-skew biases were detected for all 12 PCGs encoded by the H strand, whereas the reverse was found in MT-ND6 encoded by the L strand. One extra nucleotide'C'is present at the position 174 of MT-ND3 gene of A. fasciata, which is not observed at that of B. lagopus. Six conserved sequence boxes in the Domain II, named boxes F, E, D, C, CSBa, and CSBb, respectively, were recognized in the CRs of A. fasciata and B. lagopus. Rates and patterns of mitochondrial gene evolution within Accipitridae were also estimated. The highest dN/dS was detected for the MT-ATP8 gene (0.32493) among Accipitridae, while the lowest for the MT-CO1 gene (0.01415). Mitophylogenetic analysis supported the robust monophyly of Accipitriformes, and Cathartidae was basal to the balance of the order. Moreover, we performed phylogenetic analyses using two other data sets (two mitochondrial loci, and combined nuclear and mitochondrial loci). Our results indicate that the subfamily Aquilinae and all currently polytypic genera of this subfamily are monophyletic. These two novel mtDNA data will be useful in refining the phylogenetic relationships and evolutionary processes of Accipitriformes.
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China; The Provincial Key Laboratory of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, China.
authors: Lan
authors: L
authors: Jiang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Juan
authors: J
authors: Chen
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Ping
authors: P
authors: Wang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Qiongqiong
authors: Q
authors: Ren
authors: The College of Life Sciences, Peking University, Beijing, China.
authors: Jian
authors: J
authors: Yuan
authors: Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Department of Ecology and Agriculture Research, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, Gansu, China.
authors: Chaoju
authors: C
authors: Qian
authors: The Ningguo Museum of Natural History, Ningguo, Anhui, China.
authors: Xinghong
authors: X
authors: Hua
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Zhichun
authors: Z
authors: Guo
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Lei
authors: L
authors: Zhang
authors: The Department of Medical Biology, Wannan medical college, Wuhu, Anhui, China.
authors: Jianke
authors: J
authors: Yang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Ying
authors: Y
authors: Wang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Qin
authors: Q
authors: Zhang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Hengwu
authors: H
authors: Ding
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: De
authors: D
authors: Bi
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Zongmeng
authors: Z
authors: Zhang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Qingqing
authors: Q
authors: Wang
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
authors: Dongsheng
authors: D
authors: Chen
authors: The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China; The Provincial Key Laboratory of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, China.
authors: Xianzhao
authors: X
authors: Kan
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D005075:Biological Evolution; D004272:DNA, Mitochondrial / Q000235:genetics*; D046093:Falconiformes / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial