title: Labeling of Enveloped Virus via Metabolic Incorporation of Azido Sugars.
abstract: Modification of an enveloped measles virus was achieved by metabolic incorporation of azido sugars in host cells through the protein glycosylation process. Based on this, the resulting measles virus particles could be modified with azido groups on the surface glycoproteins, which could be further labeled with fluorescence dyes using a strain-promoted azide-alkyne cycloaddition reaction. We envision this metabolic labeling approach to be applicable to a wide variety of enveloped viruses, allowing the facile conjugation and surface modification.
authors: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.
authors: Xia
authors: X
authors: Zhao
authors: Division of Mathematics and Science, University of South Carolina Salkehatchie , Walterboro, South Carolina 29488, United States.
authors: Li
authors: L
authors: Cai
authors: Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States.
authors: Enoch A
authors: EA
authors: Adogla
authors: Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States.
authors: Hong
authors: H
authors: Guan
authors: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.
authors: Yuan
authors: Y
authors: Lin
authors: Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States.
authors: Qian
authors: Q
authors: Wang
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D061565:Cycloaddition Reaction; D006595:Hexosamines / Q000737:chemistry*; D008459:Measles virus / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000480:Alkynes; D001386:Azides; D006595:Hexosamines; C008322:mannosamine