title: Association of APC I1307K and E1317Q polymorphisms with colorectal cancer among Egyptian subjects.
abstract: Colorectal cancer is a multifactorial disease that involves both environmental and genetic factors. The gene encoding adenomatous polyposis coli (APC) has been reported to be associated with colorectal cancer (CRC) risk in several ethnic populations. The aim of this work is to assess the association of the APC I1307K and E1317Q polymorphisms with CRC risk among Egyptian subjects. This study included 120 unrelated CRC Egyptian patients who were compared to 100 healthy controls from the same locality. For all subjects, DNA was genotyped for APC I1307K and E1317Q polymorphisms using the PCR-ARMS technique. The frequency of APC I1307K carrier (TA+AA genotypes) was noted to be significantly higher among cases with CRC compared to controls (18.3 vs. 9.0 %, OR 2.58, 95 % CI 1.09-6.09, p = 0.03). Also the frequency of the APC I1307K A allele was significantly higher among cases compared to controls (10.4 vs. 4.5 %, OR 2.47; 95 % CI 1.12-5.42, p = 0.03). On the contrast, the frequencies of APC E1317Q GC genotype and C allele showed no significant difference among CRC patients compared to controls (3.3 vs. 2.0 %, OR 1.69; 95 % CI 0.30-9.42, p = 0.69 and 2.1 vs. 1.0 %, OR 2.11; 95 % CI 0.40-10.97, p = 0.46, respectively). Cases of the APC I1307K and E1317Q carriers (TA+AA and GC) showed no significant difference compared to those with I1307K and E1317Q non-carriers (TT and GG) regarding their clinical and laboratory markers. APC I1307K variant was associated with an increased risk of CRC among Egyptian subjects.
authors: Department of Biochemistry, College of Science, Damietta University, Damietta, Egypt.
authors: Camelia
authors: C
authors: Abdel-Malak
authors: Medical Oncology Department, Damietta Cancer Institute, Damietta, Egypt.
authors: Hossam
authors: H
authors: Darwish
authors: Genetics Unit, Children Hospital, Mansoura University, Mansoura, Egypt.
authors: Afaf
authors: A
authors: Elsaid
authors: Department of Biochemistry, College of Science, Damietta University, Damietta, Egypt.
authors: Fatma
authors: F
authors: El-Tarapely
authors: Department of Biochemistry, College of Science, Tanta University, Tanta, Egypt. Biolab100@gmail.com.|Medical Laboratory Department, College of Medical Applied Sciences, Majmaah University, Al Majma'ah, KSA. Biolab100@gmail.com.
authors: Rami
authors: R
authors: Elshazli
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics*; D000328:Adult; D018912:Arabs / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D004534:Egypt; D005260:Female; D017491:Genes, APC; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: C555337:APC protein, human; D025601:Adenomatous Polyposis Coli Protein
keywords: APC E1317Q; APC I1307K; Colorectal cancer; Gene polymorphism