title: Folic acid-targeted magnetic Tb-doped CeF3 fluorescent nanoparticles as bimodal probes for cellular fluorescence and magnetic resonance imaging.
abstract: Magnetic fluorescent nanoparticles (NPs) have great potential applications for diagnostics, imaging and therapy. We developed a facile polyol method to synthesize multifunctional Fe3O4@CeF3:Tb@CeF3 NPs with small size (<20 nm), high water solubility and good biocompatibility. The NPs were modified by ligand exchange reactions with citric acid (CA) to obtain carboxyl-functionalized NPs (Fe3O4@CeF3:Tb@CeF3-COOH). Folic acid (FA) as an affinity ligand was then covalently conjugated onto NPs to yield Fe3O4@CeF3:Tb@CeF3-FA NPs. They were then applied as multimodal imaging agents for simultaneous in vitro targeted fluorescence imaging and magnetic resonance imaging (MRI) of HeLa cells with overexpressed folate receptors (FR). The results indicated that these NPs had strong luminescence and enhanced T2-weighted MR contrast and would be promising candidates as multimodal probes for both fluorescence and MRI imaging.
authors: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn.
authors: Zhi-Ya
authors: ZY
authors: Ma
authors: Yu-Ping
authors: YP
authors: Liu
authors: Ling-Yu
authors: LY
authors: Bai
authors: Jie
authors: J
authors: An
authors: Lin
authors: L
authors: Zhang
authors: Yang
authors: Y
authors: Xuan
authors: Xiao-Shuai
authors: XS
authors: Zhang
authors: Yuan-Di
authors: YD
authors: Zhao
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002586:Cesium / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored / Q000235:genetics / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013725:Terbium / Q000737:chemistry*
chemical_list: D058973:Folate Receptors, GPI-Anchored; D013725:Terbium; D002586:Cesium; D005492:Folic Acid; D005459:Fluorides; C027754:cesium fluoride