title: Expression patterns of antioxidant genes in human SH-SY5Y cells after treatment with methadone.
abstract: The expression levels of nine antioxidant genes in SH-SY5Y cells exposed to methadone (final concentrations 1-20µM) were investigated. Based on this study the genes could be categorized on three different groups. The number of down-regulated genes were increased as a function of exposure time (P=0.004). The methadone associated mRNA alterations were modulated by N-acetyl-cysteine. These findings suggested that different pathways for regulation of antioxidant genes could be active after exposing of SH-SY5Y cells to methadone; and also suggested that methadone might act by inducing the reactive oxygen species.
authors: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors: Khyber
authors: K
authors: Saify
authors: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran. Electronic address: saadat@shirazu.ac.ir.
authors: Mostafa
authors: M
authors: Saadat
mesh_terms: D000975:Antioxidants / Q000502:physiology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D008691:Methadone / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D006131:Growth Inhibitors; D017382:Reactive Oxygen Species; D008691:Methadone
keywords: Antioxidant; Gene expression; Methadone