title: Adenosine Receptor Blockade by Caffeine Inhibits Carotid Sinus Nerve Chemosensory Activity in Chronic Intermittent Hypoxic Animals.
abstract: Adenosine is a key excitatory neurotransmitter at the synapse between O(2)-sensing chemoreceptor cells-carotid sinus nerve (CSN) endings in the carotid body (CB). Herein, we have investigated the significance of adenosine, through the blockade of its receptors with caffeine, on the CB hypoxic sensitization induced by chronic intermittent hypoxia (CIH) in the rat. CIH animals were obtained by submitting rats during 15 days from 8:00 to 16:00 to 10 %O(2) for 40 s and 20 % O(2) for 80 s (i.e., 30 episodes/h). Caffeine (1 mM) was tested in spontaneous and 5 %O(2) evoked-CSN chemosensory activity in normoxic and CIH animals. CIH decreased basal spontaneous activity but increased significantly CSN activity evoked by acute hypoxia. Caffeine did not modify basal spontaneous activity in normoxic rats, but decreased significantly by 47.83 % basal activity in CIH animals. In addition, acute application of caffeine decreased 49.31 % and 56.01 % the acute hypoxic response in normoxic and CIH animals, respectively. We demonstrate that adenosine contributes to fix CSN basal activity during CIH, being also involved in hypoxic CB chemotransduction. It is concluded that adenosine participates in CB sensitization during CIH.
authors: CEDOC, Centro de Estudos Doenças Crónicas, Nova Medical School, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Campo Mártires da Pátria, 130, 1169-056, Lisbon, Portugal.
authors: J F
authors: JF
authors: Sacramento
authors: C
authors: C
authors: Gonzalez
authors: M C
authors: MC
authors: Gonzalez-Martin
authors: S V
authors: SV
authors: Conde
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002344:Carotid Body / Q000187:drug effects* / Q000502:physiology; D002346:Carotid Sinus / Q000294:innervation*; D002908:Chronic Disease; D000860:Hypoxia / Q000503:physiopathology*; D058915:Purinergic P1 Receptor Antagonists / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D058915:Purinergic P1 Receptor Antagonists; D002110:Caffeine