title: Metabolomic study of urinary polyamines in rat exposed to 915 MHz radiofrequency identification signal.
abstract: Metabolomic analysis of urinary polyamines (PAs) from rat exposed to 915 MHz radiofrequency identification (RFID) signal for 8 h/day for 2 weeks was performed by gas chromatography-mass spectrometry as N-ethoxycarbonyl/N-pentafluoropropionyl derivatives. Large alterations in nine PA levels including four aliphatic and five acetylated PAs were monitored in sham-exposed and RFID-exposed groups. Total PA and urinary levels of N (1)-acetylputrescine, N (1)-acetylcadaverine, putrescine, cadaverine, N (1)-acetylspermidine, N (8)-acetylspermidine, spermidine and spermine were reduced, whereas N (1)-acetylspermine was significantly increased after sham and RFID exposure compared with those before exposure. Their levels were normalized to the corresponding group means before exposure and then plotted into star symbol patterns. N (1)-Acetylspermine after RFID exposure was 54 % higher compared to the level before RFID exposure, while it was elevated by only 17 % in the sham group. The results suggest that 915 MHz RFID exposure may induce metabolic disturbance of PA. It may also elevate spermidine/spermine acetyltransferase (SSAT) activity. Thus, the present metabolic profiling combined with star pattern recognition method might be useful for understanding the complexity of biochemical events after exposure to RFID signal.
authors: College of Pharmacy, Sunchon National University, Sunchon, 540-950, Republic of Korea. paik815@sunchon.ac.kr.
authors: Man-Jeong
authors: MJ
authors: Paik
authors: Department of Neurosurgery, Ajou University School of Medicine, Suwon, 443-749, Republic of Korea.
authors: Hye Sun
authors: HS
authors: Kim
authors: Division of Life Science and Pharaceuticals, College of Pharmacy, Ewha Woman's University, Seoul, Republic of Korea.
authors: Yun-Sil
authors: YS
authors: Lee
authors: Radio Technology Research Department, Electronics and Telecommunications Research Institute, Daejeon, Republic of Korea.
authors: Hyung Do
authors: HD
authors: Choi
authors: Department of Radio Sciences and Engineering, College of Engineering, Chungnam National University, Daejeon, Republic of Korea.
authors: Jeong-Ki
authors: JK
authors: Pack
authors: School of Electrical and Computer Engineering, Chungbuk National University, Cheongju, Republic of Korea.
authors: Nam
authors: N
authors: Kim
authors: Department of Neurosurgery, Ajou University School of Medicine, Suwon, 443-749, Republic of Korea. yhahn@ajou.ac.kr.|Neuroscience Graduate Program, Department of Biomedical Sciences, Graduate School of Ajou University, Suwon, Republic of Korea. yhahn@ajou.ac.kr.
authors: Young Hwan
authors: YH
authors: Ahn
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D008660:Metabolism / Q000528:radiation effects*; D055432:Metabolomics; D011073:Polyamines / Q000378:metabolism / Q000652:urine*; D011846:Radio Waves / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011073:Polyamines
keywords: Gas chromatography-mass spectrometry; Metabolomic analysis; Radiofrequency identification; Star pattern recognition; Urinary polyamines