title: Diagnostic value of antibody responses to multiple antigens from Mycobacterium tuberculosis in active and latent tuberculosis.
abstract: We investigated the antibody responses to 10 prospective Mycobacterium tuberculosis (MTB) antigens and evaluated their ability to discriminate between latent (LTBI) and active pulmonary tuberculosis (TB). Our results indicate that plasma levels of anti-α-crystallin (ACR), antilipoarabinomannan, anti-trehalose 6,6'-dimycolate, and anti-tubercular-glycolipid antigen antibodies were higher in patients with active TB, compared to those in the LTBI and control subjects. No differences in the antibodies were observed between the control and LTBI subjects. Antibodies against the glycolipid antigens could not distinguish between Mycobacterium avium complex (MAC)-negative TB patients and MAC-infected LTBI individuals. The most useful serological marker was antibodies to ACR, with MAC-negative TB patients having higher titers than those observed in MAC-positive LTBI and control subjects. Our data indicate that antibody to ACR is a promising target for the serological diagnosis of patients with active TB patients. When dealing with antiglycolipid antibodies, MAC coinfection should always be considered in serological studies.
authors: Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 21 Seiryo-machi, Aoba-ku, Sendai, 980-8574 Miyagi, Japan; Public Health Science Program, Faculty of Medicine, Universitas Padjadjaran, Jl. Eicjkman 38, Bandung, 40161, West Java, Indonesia. Electronic address: an2_dri@rocketmail.com.
authors: Muhammad Andrian
authors: MA
authors: Senoputra
authors: Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 21 Seiryo-machi, Aoba-ku, Sendai, 980-8574 Miyagi, Japan; Division of Disaster-related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Miyagi, Japan; Japan International Corporation of Welfare Services, 2-3-20 Toranomon YHK Bldg. 4F, Toranomon, Minato-ku, 105-0001 Tokyo, Japan. Electronic address: beatabucekova@gmail.com.
authors: Beata
authors: B
authors: Shiratori
authors: Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 21 Seiryo-machi, Aoba-ku, Sendai, 980-8574 Miyagi, Japan; Public Health Science Program, Faculty of Medicine, Universitas Padjadjaran, Jl. Eicjkman 38, Bandung, 40161, West Java, Indonesia. Electronic address: arial_dabbuss@yahoo.com.
authors: Fakhrial Mirwan
authors: FM
authors: Hasibuan
authors: TB-HIV Research Center, Medical faculty, Padjadjaran University, Jl. Eicjkman 38, Bandung, 40161, West Java, Indonesia. Electronic address: r.cundarani@gmail.com.
authors: Raspati Cundarani
authors: RC
authors: Koesoemadinata
authors: TB-HIV Research Center, Medical faculty, Padjadjaran University, Jl. Eicjkman 38, Bandung, 40161, West Java, Indonesia. Electronic address: likaaji@gmail.com.
authors: Lika
authors: L
authors: Apriani
authors: Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 21 Seiryo-machi, Aoba-ku, Sendai, 980-8574 Miyagi, Japan; Division of Disaster-related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Miyagi, Japan. Electronic address: ya82@yahoo.co.jp.
authors: Yugo
authors: Y
authors: Ashino
authors: Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, 771-0192 Tokushima, Japan. Electronic address: onok@otsuka.jp.
authors: Kenji
authors: K
authors: Ono
authors: Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, 771-0192 Tokushima, Japan. Electronic address: odate@otsuka.jp.
authors: Tetsuya
authors: T
authors: Oda
authors: Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, 771-0192 Tokushima, Japan. Electronic address: m_matsumoto@research.otsuka.co.jp.
authors: Makoto
authors: M
authors: Matsumoto
authors: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10, Kita-ku, Sapporo, 001-0020 Hokkaido, Japan. Electronic address: suzuki@czc.hokudai.ac.jp.
authors: Yasuhiko
authors: Y
authors: Suzuki
authors: TB-HIV Research Center, Medical faculty, Padjadjaran University, Jl. Eicjkman 38, Bandung, 40161, West Java, Indonesia. Electronic address: b.alisjahbana@gmail.com.
authors: Bachti
authors: B
authors: Alisjahbana
authors: Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 21 Seiryo-machi, Aoba-ku, Sendai, 980-8574 Miyagi, Japan; Division of Disaster-related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Miyagi, Japan. Electronic address: toshatto@med.tohoku.ac.jp.
authors: Toshio
authors: T
authors: Hattori
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011446:Prospective Studies; D012698:Serologic Tests / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
keywords: Lipoarabinomannan; Mycolic acid; Tubercular-glycolipid; Tuberculosis; α-Crystallin