title: Estimation of dynamic metabolic activity in micro-tissue cultures from sensor recordings with an FEM model.
abstract: We estimated the dynamic cell metabolic activity and the distribution of the pH value and oxygen concentration in tissue samples cultured in vitro by using real-time sensor records and a numerical simulation of the underlying reaction-diffusion processes. As an experimental tissue model, we used chicken spleen slices. A finite element method model representing the biochemical processes and including the relevant sensor data was set up. By fitting the calculated results to the measured data, we derived the spatiotemporal values of the pH value, the oxygen concentration and the absolute metabolic activity (extracellular acidification and oxygen uptake rate) of the samples. Notably, the location of the samples in relation to the sensors has a great influence on the detectable metabolic rates. The long-term vitality of the tissue samples strongly depends on their size. We further discuss the benefits and limitations of the model.
authors: Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik, Technische Universität München, Theresienstr. 90/N3, 80333, Munich, Germany. c.pfister@tum.de.|HP Medizintechnik GmbH, Bruckmannring 19, 85764, Oberschleißheim, Germany. c.pfister@tum.de.
authors: Cornelia
authors: C
authors: Pfister
authors: Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik, Technische Universität München, Theresienstr. 90/N3, 80333, Munich, Germany.
authors: Christian
authors: C
authors: Forstmeier
authors: Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik, Technische Universität München, Theresienstr. 90/N3, 80333, Munich, Germany.
authors: Johannes
authors: J
authors: Biedermann
authors: Institut für Tierphysiologie, Veterinärstr. 13, 80539, Munich, Germany.
authors: Julia
authors: J
authors: Schermuly
authors: Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik, Technische Universität München, Theresienstr. 90/N3, 80333, Munich, Germany.|HP Medizintechnik GmbH, Bruckmannring 19, 85764, Oberschleißheim, Germany.
authors: Franz
authors: F
authors: Demmel
authors: HP Medizintechnik GmbH, Bruckmannring 19, 85764, Oberschleißheim, Germany.
authors: Peter
authors: P
authors: Wolf
authors: Institut für Tierphysiologie, Veterinärstr. 13, 80539, Munich, Germany.
authors: Bernd
authors: B
authors: Kaspers
authors: Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik, Technische Universität München, Theresienstr. 90/N3, 80333, Munich, Germany.
authors: Martin
authors: M
authors: Brischwein
mesh_terms: D000143:Acids / Q000378:metabolism; D000818:Animals; D002645:Chickens; D005110:Extracellular Space / Q000378:metabolism; D020342:Finite Element Analysis; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D010313:Partial Pressure; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000143:Acids; D010100:Oxygen
keywords: Extracellular acidification rate; Finite element method analysis; Oxygen uptake rate; Tissue culture; Tumor model