title: Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age.
abstract: The study was focused on time-dependent expressions of paired-box transcription factor 7 (Pax7) and myoblast determination protein (MyoD) during skeletal muscle wound healing. An animal model of skeletal muscle contusion was established in 40 Sprague-Dawley male rats. Samples were taken at 1, 3, 5, 7, 9, 13, 17, and 21 days after injury, respectively (five rats in each posttraumatic interval). Five rats were employed as control. By morphometric analysis, the data based on the number of Pax7(+)/MyoD(-), Pax7(+)/MyoD(+), and Pax7(-)/MyoD(+) cells were highly correlated with the wound age. Pax7 and MyoD expressions were upregulated after injury by Western blot and quantitative real-time PCR assays. The relative quantity of Pax7 protein peaked at 5 days after injury, which was >1.13, and decreased thereafter. Similarly, the relative quantity of MyoD mRNA expression peaked at 3 days after injury, which was >2.59. The relative quantity of Pax7 protein >0.73 or mRNA expression >2.38 or the relative quantity of MyoD protein >1.33 suggested a wound age of 3 to 7 days. The relative quantity of MyoD mRNA expression >2.02 suggested a wound age of 1 to 7 days post-injury. In conclusion, the expressions of Pax7 and MyoD are upregulated in a time-dependent manner during skeletal muscle wound healing, suggesting that Pax7 and MyoD may be potential markers for wound age estimation in skeletal muscle.
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Zhi-Ling
authors: ZL
authors: Tian
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Shu-Kun
authors: SK
authors: Jiang
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Miao
authors: M
authors: Zhang
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Meng
authors: M
authors: Wang
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Jiao-Yong
authors: JY
authors: Li
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Rui
authors: R
authors: Zhao
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Lin-Lin
authors: LL
authors: Wang
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Shan-Shan
authors: SS
authors: Li
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Min
authors: M
authors: Liu
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China.
authors: Meng-Zhou
authors: MZ
authors: Zhang
authors: Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People's Republic of China. dwguan@aliyun.com.
authors: Da-Wei
authors: DW
authors: Guan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003288:Contusions / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D005455:Fluorescent Antibody Technique; D049429:Forensic Pathology; D008297:Male; D023421:Models, Animal; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D018482:Muscle, Skeletal / Q000293:injuries*; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; C530821:PAX7 protein, rat; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger
keywords: MyoD; Pax7; Satellite cell; Skeletal muscle contusion; Wound age estimation