title: Donghicola tyrosinivorans sp. nov., a tyrosine-degrading bacterium isolated from seawater.
abstract: A Gram-stain-negative, non-motile, aerobic bacterium, strain 13-93-B1T, was isolated from seawater off Jeju Island, Republic of Korea, and was subjected to polyphasic taxonomic study. Cells formed ivory colonies and were ovoid to rod-shaped. The strain was catalase-positive, oxidase-negative and grew optimally at 30 °C, in the presence of 1-2 % (w/v) NaCl and at pH 7.0-7.5. It did not synthesize bacteriochlorophyll a. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain 13-93-B1T clustered with the type strain Donghicola eburneus SW-277T (97.0 % 16S rRNA gene sequence similarity). DNA-DNA hybridization between strain 13-93-B1T and D. eburneus KCTC 12735T was 33.1 ± 1.4 % (35.2 ± 2.8 % in a reciprocal experiment). The predominant cellular fatty acid was summed feature 8 (C18 : 1ω7c/C18 : 1ω6c; 76.9 %). The major respiratory quinone was ubiquinone Q-10 and polar lipids detected in strain 13-93-B1T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and unidentified lipids. The DNA G+C content of strain 13-93-B1T was 60.4 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-93-B1T is considered to represent a novel species of the genus Donghicola, for which the name Donghicola tyrosinivorans sp. nov. is proposed. The type strain is 13-93-B1T ( = DSM 100212T = KCTC 42571T).
authors: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Hye-Ri
authors: HR
authors: Sung
authors: Department of Food Science and Technology and Basic Science Institute, Hoseo University, Asan 336-795, Republic of Korea.
authors: Jin-Man
authors: JM
authors: Lee
authors: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Mibang
authors: M
authors: Kim
authors: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Bo-Ram
authors: BR
authors: Yun
authors: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Kee-Sun
authors: KS
authors: Shin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014443:Tyrosine / Q000378:metabolism; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D014443:Tyrosine; C025204:Ubiquinone Q2