title: MiR-215, an activator of the CTNNBIP1/β-catenin pathway, is a marker of poor prognosis in human glioma.
abstract: MicroRNA-215 (miR-215) promotes tumor growth in various human malignancies. However, its role has not yet been determined in human glioma. Here, we found that levels of miR-215 were higher in glioma tissues than in corresponding non-neoplastic brain tissue. High miR-215 expression was correlated with higher World Health Organization (WHO) grades and shorter overall survival. Multivariate and univariate analysis indicated that miR-215 expression was an independent prognostic factor. We also found that TGF-beta1, phosphorylated beta-catenin, alpha-SMA, and fibronectin were increased in glioma tissues. Additionally, CTNNBIP1, a direct target of miR-215, was decreased in glioma compared to adjacent normal tissue. These data indicate that miR-215 activates Wnt/β-catenin signaling by increasing β-catenin phosphorylation, α-SMA expression, and fibronectin expression. It promotes TGF-β1-induced oncogenesis by suppressing CTNNBIP1 in glioma. In summary, miR-215 is overexpressed in human glioma, is involved in TGF-β1-induced oncogenesis, and can be used as a marker of poor prognosis in glioma patients.
authors: Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.|Clinical Molecular Diagnostic Center, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
authors: Yong-Qing
authors: YQ
authors: Tong
authors: Department of Pathology Affiliated Tianyou Hospital of Wuhan University of Science and Technology, Wuhan 430064, PR China.
authors: Bei
authors: B
authors: Liu
authors: Clinical Molecular Diagnostic Center, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
authors: Hong-Yun
authors: HY
authors: Zheng
authors: Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
authors: Jian
authors: J
authors: Gu
authors: Clinical Molecular Diagnostic Center, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
authors: Hang
authors: H
authors: Liu
authors: Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
authors: Feng
authors: F
authors: Li
authors: Pennsylvania State University College of Medicine and Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
authors: Bi-Hua
authors: BH
authors: Tan
authors: Pennsylvania State University College of Medicine and Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
authors: Melanie
authors: M
authors: Hartman
authors: Pennsylvania State University College of Medicine and Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
authors: Chunhua
authors: C
authors: Song
authors: Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.|Clinical Molecular Diagnostic Center, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
authors: Yan
authors: Y
authors: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D015153:Blotting, Western / Q000706:statistics & numerical data; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000706:statistics & numerical data; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics; D055815:Young Adult; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C411982:CTNNBIP1 protein, human; D005353:Fibronectins; D047908:Intracellular Signaling Peptides and Proteins; C553667:MIRN215 microRNA, human; D035683:MicroRNAs; D053773:Transforming Growth Factor beta1; D051176:beta Catenin
keywords: CTNNBIP1; TGF-β1; glioma; miR-215; prognosis