title: Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography.
abstract: In the present work a simple, reliable and affordable sample treatment method for the simultaneous analysis of tocopherols and free phytosterols in nuts was developed. Analyte extraction was carried out using the QuEChERS methodology and analyte separation and detection were accomplished using HPLC-DAD. The use of this methodology for the extraction of natural occurring substances provides advantages such as speed, simplicity and ease of use. The parameters evaluated for the validation of the method developed included the linearity of the calibration plots, the detection and quantification limits, repeatability, reproducibility and recovery. The proposed method was successfully applied to the analysis of tocopherols and free phytosterols in samples of almonds, cashew nuts, hazelnuts, peanuts, tiger nuts, sun flower seeds and pistachios.
authors: Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, 37008 Salamanca, Spain. Electronic address: mdz@usal.es.
authors: M Milagros
authors: MM
authors: Delgado-Zamarreño
authors: Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, 37008 Salamanca, Spain.
authors: Cristina
authors: C
authors: Fernández-Prieto
authors: Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, 37008 Salamanca, Spain.
authors: Myriam
authors: M
authors: Bustamante-Rangel
authors: Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, 37008 Salamanca, Spain.
authors: Lara
authors: L
authors: Pérez-Martín
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D009754:Nuts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D012855:Sitosterols / Q000032:analysis / Q000737:chemistry*; D024505:Tocopherols / Q000032:analysis / Q000737:chemistry*
chemical_list: D012855:Sitosterols; D024505:Tocopherols
keywords: Alpha-tocopherol (PubChem CID: 2116); Beta-sitosterol (PubChem CID: 222284); Brassicasterol (PubChem CID: 5281327); Campesterol (PubChem CID: 173183); Delta-tocopherol (PubChem CID: 92094); Gamma-tocopherol (PubChem CID: 92729); LC; Nuts; Phytosterols; QuEChERS; Stigmasterol (PubChem CID: 5280794); Tocopherols