title: Therapeutic Ultrasound Contrast Agents for the Enhancement of Tumor Diagnosis and Tumor Therapy.
abstract: The functionality of ultrasound in early cancer detection is limited because of its relatively low contrast resolution. Because it has a high degree of echogenicity, a microbubble contrast agent is often used to overcome this intrinsic limitation of imaging at low-contrast resolution. A targeted and drug-loaded microbubble contrast agent for simultaneous diagnosis and therapy has recently been investigated. However, no optimized theragnosis ultrasound microbubbles have been developed. Paclitaxel (PTX)-encapsulating human serum albumin nanoparticles (PTX-HSA-NPs) were conjugated onto an ultrasound microbubbles (PTX-HSA-NPs-MBs) fabricated in the laboratory to result in a narrow size distribution (1.7 ± 0.7 μm) and an optimal resonance frequency of 3 MHz. After intravenous injection of HSA-NPs-MBs, echogenicity in the tumor xenografted with breast cancer MCF-7 cells was significantly enhanced, showing the possibility of early cancer diagnosis. Mice injected with PTX-HSA-NPs-MBs showed higher survival rates in comparison with control groups, demonstrating the possibility of theragnosis. In the present study, the conjugation of PTX-HSA-NPs onto the ultrasound microbubbles simultaneously provided (1) enhanced ultrasound signal generation, (2) sufficient drug-loading capacity, (3) ability to deliver drugs to a preferred tumor site, and (4) increased stability in blood circulation.
authors: Hyungwon
authors: H
authors: Moon
authors: Changhan
authors: C
authors: Yoon
authors: Tae Woong
authors: TW
authors: Lee
authors: Kyoung-Su
authors: KS
authors: Ha
authors: Jin Ho
authors: JH
authors: Chang
authors: Tai-Kyong
authors: TK
authors: Song
authors: Kwangmeyung
authors: K
authors: Kim
authors: Hyuncheol
authors: H
authors: Kim
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D045423:Microbubbles / Q000627:therapeutic use*; D053769:Nanocapsules / Q000737:chemistry / Q000627:therapeutic use*; D017239:Paclitaxel / Q000008:administration & dosage*; D016896:Treatment Outcome; D014463:Ultrasonography / Q000379:methods*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003287:Contrast Media; D053769:Nanocapsules; D017239:Paclitaxel