title: Antimicrobial Peptide-Conjugated Graphene Oxide Membrane for Efficient Removal and Effective Killing of Multiple Drug Resistant Bacteria.
abstract: According to the World Health Organization (WHO), multiple drug-resistant (MDR) bacterial infection is a top threat to human health. Since bacteria evolve to resist antibiotics faster than scientists can develop new classes of drugs, the development of new materials which can be used, not only for separation, but also for effective disinfection of drug resistant pathogens is urgent. Driven by this need, we report for the first time the development of a nisin antimicrobial peptide conjugated, three dimensional (3D) porous graphene oxide membrane for identification, effective separation, and complete disinfection of MDR methicillin-resistantStaphylococcus aureus(MRSA) pathogens from water. Experimental data show that due to the size differences, MRSA is captured by the porous membrane, allowing only water to pass through. SEM, TEM, and fluorescence images confirm that pathogens are captured by the membrane. RT-PCR data with colony counting indicate that almost 100% ofMRSAcan be removed and destroyed from the water sample using the developed membrane. Comparison of MDR killing data between nisin alone, the graphene oxide membrane and the nisin attached graphene oxide membrane demonstrate that the nisin antimicrobial peptide attached graphene oxide membrane can dramatically enhance the possibility of destroying MRSA via a synergestic effect due to the multimodal mechanism.
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Rajashekhar
authors: R
authors: Kanchanapally
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Bhanu Priya
authors: BP
authors: Viraka Nellore
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Sudarson Sekhar
authors: SS
authors: Sinha
authors: Department of Physics and Astronomy, University of Texas at San Antonio, TX, USA.
authors: Francisco
authors: F
authors: Pedraza
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Stacy J
authors: SJ
authors: Jones
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Avijit
authors: A
authors: Pramanik
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Suhash Reddy
authors: SR
authors: Chavva
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Christine
authors: C
authors: Tchounwou
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Yongliang
authors: Y
authors: Shi
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Aruna
authors: A
authors: Vangara
authors: Department of Physics and Astronomy, University of Texas at San Antonio, TX, USA.
authors: Dhiraj
authors: D
authors: Sardar
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
authors: Paresh Chandra
authors: PC
authors: Ray