title: In situ strategy for bone repair by facilitated endogenous tissue engineering.
abstract: Traditional tissue engineering procedures are expensive and time consuming. Facilitated endogenous tissue engineering (FETE) provides a solution that can avoid the ex vivo culture of autologous cells and initiate in situ reparative endogenous repair processes in vivo. This method involves fabricating a porous scaffold that mimics the environment present during the bone formation process, consisting of components that provide biomimetic interfacial interactions to cells. After the scaffold is implanted, progenitor cells provided by autologous bone marrow and surrounding tissues then differentiate to bone cells under the direction of the in situ scaffold. This paper reports a biomimetic method to prepare a hierarchically structured hybrid scaffold. Bone-like nano hydroxyapatite (HA) was crystallized from a collagen and chitosan (CC) matrix to form a porous scaffold. The in vivo study demonstrates that this nanohybrid scaffold supports excellent bone repair. This means that the FETE approach, in which the cell culture portion of traditional tissue engineering takes place in vivo, can promote the intrinsic regenerative potential of endogenous tissues.
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China. Electronic address: ibptcjd@fzu.edu.cn.
authors: Jingdi
authors: J
authors: Chen
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; Institute of centre for stem cells and regenerative medicine laboratory, Liaocheng People's Hospital, Liaocheng 252000, China.
authors: Yujue
authors: Y
authors: Zhang
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Panpan
authors: P
authors: Pan
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Tiantang
authors: T
authors: Fan
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors: Mingmao
authors: M
authors: Chen
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China. Electronic address: zhangqiq@126.com.
authors: Qiqing
authors: Q
authors: Zhang
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D008297:Male; D016062:Porosity; D011817:Rabbits; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen; D048271:Chitosan; D017886:Durapatite
keywords: Bone; Endogenous; In situ; Scaffold; Tissue engineering