title: Data for ion and seed dependent fibril assembly of a spidroin core domain.
abstract: This data article includes size exclusion chromatography data of soluble eADF4(C16), an engineered spider silk variant based on the core domain sequence of the natural dragline silk protein ADF4 of Araneus diadematus, in combination with light scattering; the protein is monomeric before assembly. The assembled mature fibrils were visualized by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Sonicated fibrils were used as seeds to by-pass the nucleation lag phase in eADF4(C16) assembly. We also provide data on the sedimentation kinetics of spider silk in the presence of different NaCl concentrations revealing very slow protein aggregation in comparison to the fast assembly triggered by phosphate ions published previously [1]. Experiments in the Data article represent supporting material for our work published recently [1], which described the assembly mechanism of recombinant eADF4(C16) fibrils.
authors: Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
authors: Martin
authors: M
authors: Humenik
authors: Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
authors: Andrew M
authors: AM
authors: Smith
authors: Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
authors: Sina
authors: S
authors: Arndt
authors: Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Bayreuth Center for Colloids and Interfaces (BZKG), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Research Center Bio-Macromolecules (BIOmac), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Bayreuth Center for Molecular Biosciences (BZMB), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Bayreuth Center for Material Science (BayMAT), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
authors: Thomas
authors: T
authors: Scheibel
keywords: Fibril; Nucleus; Recombinant spider silk; Seed