title: Lower Oncogenic Potential of Human Mesenchymal Stem Cells Derived from Cord Blood Compared to Induced Pluripotent Stem Cells.
abstract: BACKGROUND
  In regenerative medicine, use of each of the mesenchymal stem cells derived from bone marrow, cord blood, and adipose tissue, has several cons and pros. Mesenchymal stem cells derived from cord blood have been considered the best source for precursor transplantation. Direct reprogramming of a somatic cell into induced pluripotent stem cells by over-expression of 6 transcription factors Oct4, Sox2, Klf4, lin28, Nanog, and c-Myc has great potential for regenerative medicine, eliminating the ethical issues of embryonic stem cells and the rejection problems of using non-autologous cells.
  
  
  OBJECTIVE
  To compare reprogramming and pluripotent markers OCT4, Sox-2, c-Myc, Klf4, Nanog, and lin28 in mesenchymal stem cells derived from cord blood and induced pluripotent stem cells.
  
  
  METHODS
  We analyzed the expression level of OCT4, Sox-2, c-Myc, Klf4, Nanog and lin28 genes in human mesenchymal stem cells derived from cord blood and induced pluripotent stem cells by cell culture and RT-PCR.
  
  
  RESULTS
  The expression level of pluripotent genes OCT4 and Sox-2, Nanog and lin28 in mesenchymal stem cells derived from cord blood were significantly higher than those in induced pluripotent stem cells. In contrast to OCT-4A and Sox-2, Nanog and lin28, the expression level of oncogenic factors c-Myc and Klf4 were significantly higher in induced pluripotent stem cells than in mesenchymal stem cells derived from cord blood.
  
  
  CONCLUSIONS
  It could be concluded that mesenchymal stem cells derived from human cord blood have lower oncogenic potential compared to induced pluripotent stem cells.
authors: Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University.
authors: T
authors: T
authors: Foroutan
authors: Department of Cell Biology, Faculty of Sciences and New Technologies, Pharmaceutical Sciences Branch of Islamic Azad University.
authors: M
authors: M
authors: Najmi
authors: Department of Cell Biology, Faculty of Sciences and New Technologies, Pharmaceutical Sciences Branch of Islamic Azad University.
authors: N
authors: N
authors: Kazemi
authors: Department of Molecular Genetic, Faculty of Biological Sciences, Tarbiat Modares University.
authors: M
authors: M
authors: Hasanlou
authors: Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University.
authors: A
authors: A
authors: Pedram
keywords: Cord blood; Mesenchymal stem cells; OCT4; Sox2; c-Myc; Nanog; lin28