title: Galectin-9 in Combination With EX-527 Prolongs the Survival of Cardiac Allografts in Mice After Cardiac Transplantation.
abstract: Galectin-9 (Gal-9), a member of the galectin family, has a variety of biologic activities. However, its role in allografts is not fully clarified yet. The relationship between interleukin-17 (IL-17) and Gal-9 and the role of Gal-9 in T(H)17-cell differentiation also remain unclear. We built a murine cardiac transplantation model, which we treated with Gal-9 and/or EX-527, a specific Sirtuin-1 inhibitor. Afterwards, flow-cytometric analysis and reverse-transcription polymerase chain reaction were used to detect the number of T(H)17 cells and the expression of key factors involved in the differentiation of T(H)17 cells; in addition, the survival times of cardiac transplanted mice in different groups were recorded. The levels of circulating T(H)17 cells were found to increase in the peripheral blood of mice that exhibited acute rejection (AR) after heart transplantation, which was determined to be correlated with the rejection grade. Gal-9 or EX-527 can inhibit the activation and differentiation of T(H)17 cells and effectively suppress T(H)17-cell-mediated AR. These data provide new evidence for the potential regulatory effects of Gal-9 in alloimmune responses in a murine model of heart transplantation, and suggest the combined use of galectin-9 and EX-527 may be a powerful method to induce tolerance of fully mismatched murine cardiac allografts.
authors: Department of General Surgery and Liver Transplant Center, Huashan Hospital, Fudan University, Shanghai, China.
authors: Y-f
authors: YF
authors: Tao
authors: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang, China.
authors: F
authors: F
authors: Lin
authors: Peking University Clinical Research Institute, Health Science Center, Peking University, Beijing, China.
authors: X-y
authors: XY
authors: Yan
authors: Division of Liver Transplantation, Organ Transplant Center, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: X-g
authors: XG
authors: Gao
authors: Division of Liver Transplantation, Organ Transplant Center, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: F
authors: F
authors: Teng
authors: Division of Liver Transplantation, Organ Transplant Center, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Z-r
authors: ZR
authors: Fu
authors: Department of General Surgery and Liver Transplant Center, Huashan Hospital, Fudan University, Shanghai, China. Electronic address: wangzxkxy@126.com.
authors: Z-x
authors: ZX
authors: Wang
mesh_terms: D064591:Allografts; D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D004195:Disease Models, Animal; D037161:Galectins / Q000494:pharmacology*; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D006085:Graft Survival; D016027:Heart Transplantation / Q000379:methods*; D007108:Immune Tolerance; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014184:Transplantation, Homologous
chemical_list: C550547:6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide; D002227:Carbazoles; D037161:Galectins; C508186:galectin 9, mouse