title: MhNCED3 in Malus hupehensis Rehd. induces NO generation under osmotic stress by regulating ABA accumulation.
abstract: Abscisic acid (ABA) biosynthesis has been widely characterized in plants, whereas the effects of ABA biosynthesis on nitric oxide (NO) generation in osmotic stress are less well understood. In this study, Malus hupehensis Rehd. 9-cis-epoxycarotenoid dioxygenase gene (MhNCED3) which is the key gene in ABA biosynthesis was transformed into wild type (WT) and 129B08/nced3 mutant (AtNCED3 deficient), respectively, and two transgenic Arabidopsis lines were obtained. The transgenic Arabidopsis lines displayed higher endogenous ABA content, NO generation rate, AtNIA1 transcript level and nitrate reductase (NR) activity than WT and 129B08/nced3 mutant. Ectopic expression of MhNCED3 reduced the electrolyte leakage and relieved Arabidopsis damage caused by 20% PEG on the growth and development. The ABA content, NO generation rate, AtNIA1 expression and NR activity increased after 20% PEG treatment, importantly, their increases amplitude relative to that in control were higher in two transgenic lines. Additionally, during the treatment for the four genotype Arabidopsis, the time of ABA contents reaching the highest peak was earlier than the time of NO generation, AtNIA1 expression and NR activity reaching their highest peak. These results show that NCED gene indirectly induced endogenous NO generation in osmotic-stressed Arabidopsis partially contributing to the up-regulation of AtNIA1 expression and NR activity.
authors: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
authors: Wei-wei
authors: WW
authors: Zhang
authors: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China. Electronic address: hqyang@sdau.edu.cn.
authors: Hong-qiang
authors: HQ
authors: Yang
authors: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
authors: Shu-zhen
authors: SZ
authors: You
authors: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
authors: Kun
authors: K
authors: Ran
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism; D027845:Malus / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000378:metabolism; D009997:Osmotic Pressure
chemical_list: D009569:Nitric Oxide; D000040:Abscisic Acid
keywords: ABA; Malus hupehensis Rehd.; MhNCED3; Nitric oxide; Osmotic stress