title: Bacterial cell motility of Burkholderia gut symbiont is required to colonize the insect gut.
abstract: We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-l-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile ΔamiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, ΔamiC mutant cells were unable to establish symbiotic association. But, ΔamiC mutant complemented with amiC gene restored in vivo symbiotic association. ΔamiC mutant cultured in minimal medium restored their motility with single-celled morphology. When ΔamiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont.
authors: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors: Jun Beom
authors: JB
authors: Lee
authors: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors: Jin Hee
authors: JH
authors: Byeon
authors: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors: Ho Am
authors: HA
authors: Jang
authors: Department of Microbiology, Kosin University College of Medicine, Busan 602-703, South Korea.
authors: Jiyeun Kate
authors: JK
authors: Kim
authors: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors: Jin Wook
authors: JW
authors: Yoo
authors: Bioproduction Research Institute, Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo 062-8517, Japan.
authors: Yoshitomo
authors: Y
authors: Kikuchi
authors: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea. Electronic address: brlee@pusan.ac.kr.
authors: Bok Luel
authors: BL
authors: Lee
mesh_terms: D000818:Animals; D019117:Burkholderia / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D020062:Heteroptera / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000172:deficiency / Q000235:genetics; D010641:Phenotype; D017384:Sequence Deletion; D013559:Symbiosis
chemical_list: D009238:N-Acetylmuramoyl-L-alanine Amidase
keywords: AmiC; Burkholderia; N-acetylmuramyl-l-alanine amidase; Riptortus pedestris; Symbiosis