title: Spreading of heterochromatin and karyotype differentiation in two Tropidacris Scudder, 1869 species (Orthoptera, Romaleidae).
abstract: Tropidacris Scudder, 1869 is a genus widely distributed throughout the Neotropical region where speciation was probably promoted by forest reduction during the glacial and interglacial periods. There are no cytogenetic studies of Tropidacris, and information allowing inference or confirmation of the evolutionary events involved in speciation within the group is insufficient. In this paper, we used cytogenetic markers in two species, Tropidacriscollaris (Stoll, 1813) and Tropidacriscristatagrandis (Thunberg, 1824), collected in different Brazilian biomes. Both species exhibited 2n=24,XX for females and 2n=23,X0 for males. All chromosomes were acrocentric. There were some differences in the karyotype macrostructure, e.g. in the chromosome size. A wide interspecific variation in the chromosome banding (C-banding and CMA3/DAPI staining) indicated strong differences in the distribution of repetitive DNA sequences. Specifically, Tropidacriscristatagrandis had a higher number of bands in relation to Tropidacriscollaris. FISH with 18S rDNA revealed two markings coinciding with the NORs in both species. However, two analyzed samples of Tropidacriscollaris revealed a heterozygous condition for the rDNA site of S10 pair. In Tropidacriscollaris, the histone H3 genes were distributed on three chromosome pairs, whereas in Tropidacriscristatagrandis, these genes were observed on 14 autosomes and on the X chromosome, always in terminal regions. Our results demonstrate that, although the chromosome number and morphology are conserved in the genus, Tropidacriscristatagrandis substantially differs from Tropidacriscollaris in terms of the distribution of repetitive sequences. The devastation and fragmentation of the Brazilian rainforest may have led to isolation between these species, and the spreading of these repetitive sequences could contribute to speciation within the genus.
authors: Departamento de Biologia, ICB, Universidade de Pernambuco, Recife, Pernambuco, Brazil.
authors: Marília de França
authors: Mde F
authors: Rocha
authors: Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil.
authors: Mariana Bozina
authors: MB
authors: Pine
authors: Departamento de Genética, CCB, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
authors: Elizabeth Felipe Alves Dos Santos
authors: EF
authors: Oliveira
authors: Departamento de Genética, CCB, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
authors: Vilma
authors: V
authors: Loreto
authors: Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil.
authors: Raquel Bozini
authors: RB
authors: Gallo
authors: Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil.
authors: Carlos Roberto Maximiano
authors: CR
authors: da Silva
authors: Museu de Zoologia, Instituto de Biociência, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
authors: Fernando Campos
authors: FC
authors: de Domenico
authors: Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil.
authors: Renata
authors: R
authors: da Rosa
keywords: 18S rDNA; Chromosome banding; histone H3 gene; repetitive DNA; speciation