title: Involvement of Antizyme Characterized from the Small Abalone Haliotis diversicolor in Gonadal Development.
abstract: The small abalone Haliotis diversicolor is an economically important mollusk that is widely cultivated in Southern China. Gonad precocity may affect the aquaculture of small abalone. Polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. Ornithine decarboxylase (ODC) and antizyme (AZ) are essential elements of a feedback circuit that regulates cellular polyamines. This paper presents the molecular cloning and characterization of AZ from small abalone. Sequence analysis showed that the cDNA sequence of H. diversicolor AZ (HdiODCAZ) consisted of two overlapping open reading frames (ORFs) and conformed to the +1 frameshift property of the frame. Thin Layer chromatography (TLC) analysis suggested that the expressed protein encoded by +1 ORF2 was the functional AZ that targets ODC to 26S proteasome degradation. The result demonstrated that the expression level of AZ was higher than that of ODC in the ovary of small abalone. In addition, the expression profiles of ODC and AZ at the different development stages of the ovary indicated that these two genes might be involved in the gonadal development of small abalone.
authors: Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan Province, China.
authors: Wei-Dong
authors: WD
authors: Li
authors: Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan Province, China.
authors: Min
authors: M
authors: Huang
authors: College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian Province, China.
authors: Wen-Gang
authors: WG
authors: Lü
authors: Guangxi Key Lab for Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Beihai, Guangxi Province, China.
authors: Xiao
authors: X
authors: Chen
authors: Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan Province, China.
authors: Ming-Hui
authors: MH
authors: Shen
authors: Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan Province, China.
authors: Xiang-Min
authors: XM
authors: Li
authors: Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan Province, China.
authors: Rong-Xia
authors: RX
authors: Wang
authors: College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian Province, China.
authors: Cai-Huan
authors: CH
authors: Ke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D049851:Gastropoda / Q000201:enzymology / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010053:Ovary / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000502:physiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
chemical_list: D011073:Polyamines; D011506:Proteins; C031017:ornithine decarboxylase antizyme