title: Quantitative response relationships between degradation rates and functional genes during the degradation of beta-cypermethrin in soil.
abstract: In the present study, the degradation mechanisms of beta-cypermethrin and its metabolites in soil were explored through the quantitative response relationships between the degradation rates and related functional genes. We found that the degradation rate of beta-cypermethrin was rapid in unsterilized soil but not in sterilized soil, which indicated that the degradation process is microbially based. Moreover, three metabolites (3-phenoxybenzoic acid, phenol and protocatechuic acid) were detected during the degradation process and used to identify the degradation pathway and functional genes related to the degradation process. The key rate-limiting functional genes were pytH and pobA, and the relative contributions of these genes to the degradation process were examined with a path analysis. The path analysis revealed that the genes pobA and pytH had the greatest direct effects on the degradation of beta-cypermethrin (pobA), alpha-cypermethrin (pobA), theta-cypermethrin (pytH) and 3-phenoxybenzoic acid (pytH).
authors: Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871, China; Department of Plant Protection, College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Zhong-Hua
authors: ZH
authors: Yang
authors: Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871, China. Electronic address: jiguodong@iee.pku.edu.cn.
authors: Guo-Dong
authors: GD
authors: Ji
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007306:Insecticides / Q000737:chemistry*; D011722:Pyrethrins / Q000737:chemistry*; D012989:Soil Pollutants / Q000737:chemistry*
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D012989:Soil Pollutants; C017160:cypermethrin
keywords: Degradation mechanism; Functional gene; Pesticide; Process products; Soil