title: Involvement of the flagellar assembly pathway in Vibrio alginolyticus adhesion under environmental stresses.
abstract: Adhesion is an important virulence factor of Vibrio alginolyticus. This factor may be affected by environmental conditions; however, its molecular mechanism remains unclear. In our previous research, adhesion deficient strains were obtained by culturing V. alginolyticus under stresses including Cu, Pb, Hg, and low pH. With RNA-seq and bioinformatics analysis, we found that all of these stress treatments significantly affected the flagellar assembly pathway, which may play an important role in V. alginolyticus adhesion. Therefore, we hypothesized that the environmental stresses of the flagellar assembly pathway may be one way in which environmental conditions affect adhesion. To verify our hypothesis, a bioinformatics analysis, QPCR, RNAi, in vitro adhesion assay and motility assay were performed. Our results indicated that (1) the flagellar assembly pathway was sensitive to environmental stresses, (2) the flagellar assembly pathway played an important role in V. alginolyticus adhesion, and (3) motility is not the only way in which the flagellar assembly pathway affects adhesion.
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Lu
authors: L
authors: Wang
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Lixing
authors: L
authors: Huang
authors: College of Ocean and Earth Sciences, Xiamen University Xiamen, China.
authors: Yongquan
authors: Y
authors: Su
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Yingxue
authors: Y
authors: Qin
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Wendi
authors: W
authors: Kong
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Ying
authors: Y
authors: Ma
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Xiaojin
authors: X
authors: Xu
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Mao
authors: M
authors: Lin
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Jiang
authors: J
authors: Zheng
authors: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University Xiamen, China.
authors: Qingpi
authors: Q
authors: Yan
mesh_terms: D001422:Bacterial Adhesion; D019295:Computational Biology; D005407:Flagella / Q000378:metabolism*; D020869:Gene Expression Profiling; D020868:Gene Silencing; D008124:Locomotion; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D044149:Vibrio alginolyticus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
keywords: Vibrio alginolyticus; adhesion; environmental stresses; flagellar assembly pathway