title: MRI tracking of bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy.
abstract: Transplantation of bone marrow mesenchymal stem cells (BMSCs) is a promising approach for treatment of epilepsy. To our knowledge, there is little research on magnetic resonance imaging (MRI) tracking of BMSCs labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles in a rat model of temporal lobe epilepsy (TLE). In this study, BMSCs were pre-labeled with USPIO nanoparticles, and then the cell apoptosis, proliferation, surface antigens, and multipotency were investigated. Lithium chloride-pilocarpine induced TLE models were administered by USPIO-labeled BMSCs (U-BMSCs), BMSCs, and saline through lateral ventricle injection as the experimental group, control I group and control II group, respectively, followed by MRI examination, electroencephalography (EEG) and Prussian blue staining. The cell experimental results showed that the labeled USPIO did not affect the biological characteristics and multiple potential of BMSCs. The U-BMSCs can be detected using MRI in vitro and in vivo, and observed in the hippocampus and adjacent parahippocampal cortical areas of the epileptic model. Moreover, electroencephalographic results showed that transplanted U-BMSCs, as well as BMSCs, were capable of reducing the number of epileptiform waves significantly (P<0.01) compared with control II group. All of these findings suggest that it is feasible to track transplanted BMSCs using MRI in a rat model of TLE, and support that USPIO labeling is a valuable tool for cell tracking in the study of seizure disorders.
authors: Department of Neurosurgery, Xi'an Central Hospital, Affiliated Hospital of Medical College of Xi'an Jiao Tong University, No. 185 Houzai Gate of North Street, Xi'an 710003, PR China.
authors: Qianfa
authors: Q
authors: Long
authors: Department of Neurosurgery, Xi'an Central Hospital, Affiliated Hospital of Medical College of Xi'an Jiao Tong University, No. 185 Houzai Gate of North Street, Xi'an 710003, PR China.
authors: Jianying
authors: J
authors: Li
authors: Department of Neurosurgery, Xi'an Central Hospital, Affiliated Hospital of Medical College of Xi'an Jiao Tong University, No. 185 Houzai Gate of North Street, Xi'an 710003, PR China.
authors: Qiang
authors: Q
authors: Luo
authors: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, No.17 Changle West Road, Xi'an 710032, PR China.
authors: Yue
authors: Y
authors: Hei
authors: Department of Neurosurgery, Qingdao 401Hospital of PLA, No. 22 Minjiang Road, Qingdao 266071, PR China.
authors: Kai
authors: K
authors: Wang
authors: Department of Neurosurgery, Xi'an Central Hospital, Affiliated Hospital of Medical College of Xi'an Jiao Tong University, No. 185 Houzai Gate of North Street, Xi'an 710003, PR China.
authors: Ye
authors: Y
authors: Tian
authors: Department of Neurosurgery, Xi'an Central Hospital, Affiliated Hospital of Medical College of Xi'an Jiao Tong University, No. 185 Houzai Gate of North Street, Xi'an 710003, PR China.
authors: Junle
authors: J
authors: Yang
authors: Institute of Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201403, PR China.
authors: Hulong
authors: H
authors: Lei
authors: Department of Radiology, University of Washington School of Medicine, Box 357115, Seattle, WA 98195, USA. Electronic address: bqiu@u.washington.edu.
authors: Bensheng
authors: B
authors: Qiu
authors: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, No.17 Changle West Road, Xi'an 710032, PR China. Electronic address: liuwp@fmmu.edu.cn.
authors: Weiping
authors: W
authors: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D004833:Epilepsy, Temporal Lobe / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy; D005240:Feasibility Studies; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D059346:Magnets; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D017207:Rats, Sprague-Dawley
chemical_list: D058185:Magnetite Nanoparticles
keywords: Bone marrow mesenchymal stem cells; Magnetic resonance imaging; Temporal lobe epilepsy; Ultrasmall superparamagnetic iron oxide