title: Nicotiana sylvestris calcineurin B-like protein NsylCBL10 enhances salt tolerance in transgenic Arabidopsis.
abstract: Nicotiana sylvestris calcineurin B-like protein NsylCBL10 improves tolerance to high-salt stress through better maintenance of Na (+) balance. The calcineurin B-like (CBL) proteins represent a unique group of plant calcium sensors and play an important role in regulating the response of a plant cell to the stress. Although many studies have been made in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa) and poplar (Populus trichocarpa), the characterization and elucidation of the functions of CBLs in tobacco have not yet been reported. In this study, NsylCBL10, a CBL gene showing higher similarities to other CBL10 genes, was cloned from Nicotiana sylvestris. NsylCBL10 is expressed in most of the tobacco tissues, and the protein targets to the plasma membrane specifically. Over-expression of NsylCBL10 enhanced the salt tolerance of Arabidopsis wild type plants greatly, and rescued the high-salt-sensitive phenotype of Arabidopsis cbl10 mutant. The analysis of ion content indicated that over-expressing NsylCBL10 in plants is able to maintain a lower Na(+)/K(+) ratio in roots and higher Na(+)/K(+) ratio in shoots, compared with cbl10 mutant. The results suggest that NsylCBL10 might play an important role in response to high salinity stress in N. sylvestris, by keeping a better ionic homeostasis to reduce the damage of toxic ion to the plant cell.
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Lianhong
authors: L
authors: Dong
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Qian
authors: Q
authors: Wang
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: S M Nuruzzaman
authors: SM
authors: Manik
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Yufeng
authors: Y
authors: Song
authors: College of Agriculture and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, China.
authors: Sujuan
authors: S
authors: Shi
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Yulong
authors: Y
authors: Su
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China. liuguanshan@caas.cn.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China. liuguanshan@caas.cn.
authors: Guanshan
authors: G
authors: Liu
authors: Key Laboratory of Tobacco Biology and Processing, Tobacco Research Institute of CAAS, Ministry of Agriculture, Qingdao, 266101, China. liuhaobao@caas.cn.|Chinese Academy of Agricultural Sciences, Beijing, 100081, China. liuhaobao@caas.cn.
authors: Haobao
authors: H
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D015870:Gene Expression; D017930:Genes, Reporter; D006706:Homeostasis; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D055049:Salt Tolerance; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000502:physiology
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride; D019703:Calcineurin
keywords: Arabidopsis; CBL10; Nicotiana sylvestris; Salt stress; Salt tolerance