title: Enzymatic hydrolysis of ovomucin and the functional and structural characteristics of peptides in the hydrolysates.
abstract: Ovomucin was hydrolyzed using enzymes or by heating under alkaline conditions (pH 12.0), and the functional, structural and compositional characteristics of the peptides in the hydrolysates were determined. Among the treatments, heating at 100 °C for 15 min under alkaline conditions (OM) produced peptides with the highest iron-binding and antioxidant capacities. Ovomucin hydrolyzed with papain (OMPa) or alcalase (OMAl) produced peptides with high ACE-inhibitory activity. The mass spectrometry analysis indicated that most of the peptides from OMPa were <2 kDa, but peptides from OMTr and OM were >2 kDa. OMAl hydrolyzed ovomucin almost completely and no peptides within 700-5000 Da were found in the hydrolasate. The results indicated that the number and size of peptides were closely related to the functionality of the hydrolysates. Considering the time, cost and activities of the hydrolysates, OM was the best treatment for hydrolyzing ovomucin to produce functional peptides.
authors: Department of Animal Science, Uva Wellassa University, Badulla 90000, Sri Lanka.
authors: E D N S
authors: ED
authors: Abeyrathne
authors: Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: H Y
authors: HY
authors: Lee
authors: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: C
authors: C
authors: Jo
authors: Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.
authors: J W
authors: JW
authors: Suh
authors: Department of Animal Science, Iowa State University, Ames, IA 50011, USA; Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, South Korea. Electronic address: duahn@iastate.edu.
authors: D U
authors: DU
authors: Ahn
mesh_terms: D000975:Antioxidants; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods*; D010059:Ovomucin / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010455:Peptides; D010059:Ovomucin
keywords: Enzyme hydrolysis; Functional properties; Ovomucin; Peptides