title: Rarimicrobium hominis gen. nov., sp. nov., representing the fifth genus in the phylum Synergistetes that includes human clinical isolates.
abstract: Five human clinical isolates of an unknown, strictly anaerobic, slow-growing, Gram-stain-negative, rod-shaped micro-organism were subjected to a polyphasic taxonomic study. Comparative 16S rRNA gene sequence-based phylogeny showed that the isolates grouped in a clade that included members of the genera Pyramidobacter, Jonquetella, and Dethiosulfovibrio; the type strain of Pyramidobacter piscolens was the closest relative with 91.5-91.7 % 16S rRNA gene sequence similarity. The novel strains were mainly asaccharolytic and unreactive in most conventional biochemical tests. Major metabolic end products in trypticase/glucose/yeast extract broth were acetic acid and propionic acid and the major cellular fatty acids were C13 : 0 and C16 : 0, each of which could be used to differentiate the strains from P. piscolens. The DNA G+C content based on whole genome sequencing for the reference strain 22-5-S 12D6FAA was 57 mol%. Based on these data, a new genus, Rarimicrobium gen. nov., is proposed with one novel species, Rarimicrobium hominis sp. nov., named after the exclusive and rare finding of the taxon in human samples. Rarimicrobium is the fifth genus of the 14 currently characterized in the phylum Synergistetes and the third one in subdivision B that includes human isolates. The type strain of Rarimicrobium hominis is ADV70T ( = LMG 28163T = CCUG 65426T).
authors: Université de Montpellier, Unité Mixte de Recherche 5569 - Hydrosciences Montpellier, Equipe Pathogènes Hydriques Santé Environnements, Unité de Bactériologie, Faculté de Pharmacie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.|Centre Hospitalier Universitaire de Montpellier, Laboratoire d'Hygiène Hospitalière, 778 rue de la croix verte, 34295 Montpellier Cedex 5, France.
authors: Estelle
authors: E
authors: Jumas-Bilak
authors: Institut Pasteur, Centre National de Référence des Bactéries Anaérobies et du Botulisme, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors: Philippe
authors: P
authors: Bouvet
authors: Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors: Emma
authors: E
authors: Allen-Vercoe
authors: Université de Montpellier, Unité Mixte de Recherche 5569 - Hydrosciences Montpellier, Equipe Pathogènes Hydriques Santé Environnements, Unité de Bactériologie, Faculté de Pharmacie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.
authors: Fabien
authors: F
authors: Aujoulat
authors: Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
authors: Paul A
authors: PA
authors: Lawson
authors: Université de Montpellier, Unité Mixte de Recherche 5569 - Hydrosciences Montpellier, Equipe Pathogènes Hydriques Santé Environnements, Unité de Bactériologie, Faculté de Pharmacie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.|Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors: Hélène
authors: H
authors: Jean-Pierre
authors: Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.|Université de Montpellier, Unité Mixte de Recherche 5569 - Hydrosciences Montpellier, Equipe Pathogènes Hydriques Santé Environnements, Unité de Bactériologie, Faculté de Pharmacie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.
authors: Hélène
authors: H
authors: Marchandin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S