title: Prognosis for Survival of Young Women with Breast Cancer by Quantitative p53 Immunohistochemistry.
abstract: p53 protein detected immunohistochemically has not been accepted as a biomarker for breast cancer patients because of disparate reports of the relationship between the amount of p53 protein detected and patient survival. The purpose of this study was to determine experimental conditions and methods of data analysis for which p53 stain intensity could be prognostic for survival of young breast cancer patients. A tissue microarray of specimens from 93 patients was stained with anti-p53 antibody, and stain intensity measured with a computer-aided image analysis system. A cut-point at one standard deviation below the mean of the distribution of p53 stain intensity separated patients into two groups with significantly different survival. These results were confirmed by Quantitative Nuclear Grade determined by DNA-specific Feulgen staining. P53 provided information beyond ER and PR status. Therefore, under the conditions reported here, p53 protein can be an effective prognostic factor for young breast cancer patients.
authors: Department of Genetics and Cancer Institute of New Jersey, Rutgers University, New Jersey, USA.
authors: David E
authors: DE
authors: Axelrod
authors: Department of Genetics, Rutgers University, USA.
authors: Kinsuk
authors: K
authors: Shah
authors: Department of Radiation Oncology, Robert Wood Johnson Medical School-UMDNJ, Cancer Institute of New Jersey, New Jersey, USA.
authors: Qifeng
authors: Q
authors: Yang
authors: Department of Radiation Oncology, Robert Wood Johnson Medical School-UMDNJ, Cancer Institute of New Jersey, New Jersey, USA.
authors: Bruce G
authors: BG
authors: Haffty
keywords: biomarker; breast cancer; image analysis; nuclear grade; p53; prognosis; survival; tissue microarray