title: Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer.
abstract: Lymph node metastasis is an important factor determining the outcome of colorectal cancer. Although epithelial-to-mesenchymal transition (EMT), TNF-α and microRNA (miRNA) have been found to play important roles in lymph node metastasis, the underlying molecular mechanism remains unclear. Here we reported that high expression of microRNA-19a (miR-19a) was associated with lymph node metastasis and played an important role in TNF-α-induced EMT in colorectal cancer (CRC) cells. We analyzed miR-19a expression in surgical tissue specimens from 11 CRC patients and 275 formalin-fixed, paraffin-embedded CRC patients. We found that miR-19a was up-regulated in CRC tissues and high expression of miR-19a was significantly associated with lymph node metastasis. We further analyzed miR-19a lymph node metastasis signature in an external validation cohort of 311 CRC cases of the TCGA. MiR-19a was found to be significantly associated with lymph node metastasis in rectal cancer. In vitro, we showed that overexpression of miR-19a in human CRC cell lines promoted cell invasion and EMT. Furthermore, miR-19a was up-regulated by TNF-α and miR-19a was required for TNF-α-induced EMT and metastasis in CRC cells. Collectively, miR-19a played an important role in mediating EMT and metastatic behavior in CRC. It may serve as a potential marker of lymph node metastasis.
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Lanlan
authors: L
authors: Huang
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Xiaoyan
authors: X
authors: Wang
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Chuangyu
authors: C
authors: Wen
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Xiangling
authors: X
authors: Yang
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Minmin
authors: M
authors: Song
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Junxiong
authors: J
authors: Chen
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Chenliang
authors: C
authors: Wang
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.
authors: Bo
authors: B
authors: Zhang
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.
authors: Lei
authors: L
authors: Wang
authors: Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Aikichi
authors: A
authors: Iwamoto
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.
authors: Jianping
authors: J
authors: Wang
authors: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital.|Guangdong Key Laboratory of Colorectal and Pelvic Floor Diseases.|Institute of Human Virology and Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University Guangzhou, China.
authors: Huanliang
authors: H
authors: Liu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha