title: Data set for mass spectrometric analysis of recombinant human serum albumin from various expression systems.
abstract: Human serum albumin (HSA) is a versatile and important protein for the pharmaceutical industry (Fanali et al., Mol. Aspects Med. 33(3) (2012) 209-290). Due to the potential transmission of pathogens from plasma sourced albumin, numerous expression systems have been developed to produce recombinant HSA (rHSA) (Chen et al., Biochim. Biophys. Acta (BBA)-Gen. Subj. 1830(12) (2013) 5515-5525; Kobayashi, Biologicals 34(1) (2006) 55-59). Based on our previous study showing increased glycation of rHSA expressed in Asian rice (Frahm et al., J. Phys. Chem. B 116(15) (2012) 4661-4670), both supplier-to-supplier and lot-to-lot variability of rHSAs from a number of expression systems were evaluated using reversed phase liquid chromatography linked with MS and MS/MS analyses. The data are associated with the research article 'Determination of Supplier-to-Supplier and Lot-to-Lot Variability in Glycation of Recombinant Human Serum Albumin Expressed in Oryza sativa' where further analysis of rHSA samples with additional biophysical methods can be found (Frahm et al., PLoS ONE 10(9) (2014) e109893). We determined that all rHSA samples expressed in rice showed elevated levels of arginine and lysine hexose glycation compared to rHSA expressed in yeast, suggesting that the extensive glycation of the recombinant proteins is a by-product of either the expression system or purification process and not a random occurrence.
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Daryl G S
authors: DG
authors: Smith
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Grant E
authors: GE
authors: Frahm
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Anita
authors: A
authors: Kane
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Barry
authors: B
authors: Lorbetskie
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Michel
authors: M
authors: Girard
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Michael J W
authors: MJ
authors: Johnston
authors: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directoriate, Health Canada, Ottawa, ON, Canada K1A0K9.
authors: Terry D
authors: TD
authors: Cyr
keywords: Albumin; Glycation; Mass spectrometry; Oryza sativa; Recombinant proteins