title: Dysregulated expression of sterol O-acyltransferase 1 (Soat1) in the hair shaft of Hoxc13 null mice.
abstract: The cholesterol-metabolizing enzyme sterol O-acetyltransferase (SOAT1) is implicated in an increasing number of biological and pathological processes in a number of organ systems, including the differentiation of the hair shaft. While the functional and regulatory mechanisms underlying these diverse functional roles remain poorly understood, the compartment of the hair shaft known as medulla, affected by mutations in Soat1, may serve as a suitable model for defining some of these mechanisms. A comparative analysis of mRNA and protein expression patterns of Soat1/SOAT1 and the transcriptional regulator Hoxc13/HOXC13 in postnatal skin of FVB/NTac mice indicated co-expression in the most proximal cells of the differentiating medulla. This finding combined with the significant downregulation of Soat1 expression in postnatal skin of both Hoxc13 gene-targeted and transgenic mice based on previously reported DNA microarray results suggests a potential regulatory relationship between the two genes. Non-detectable SOAT1 expression in the defective hair follicle medulla of Hoxc13(tm1Mrc) mice and evidence for binding of HOXC13 to the Soat1 upstream control region obtained by ChIP assay suggests that Soat1 is a downstream regulatory target for HOXC13 during medulla differentiation.
authors: Department of Medicine, Medical University of South Carolina, Charleston, SC, United States.
authors: Christopher S
authors: CS
authors: Potter
authors: Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, United States.
authors: Michael J
authors: MJ
authors: Kern
authors: Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, United States.
authors: Mary Ann
authors: MA
authors: Baybo
authors: Department of Medicine, Medical University of South Carolina, Charleston, SC, United States.
authors: Nathanael D
authors: ND
authors: Pruett
authors: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.
authors: Alan R
authors: AR
authors: Godwin
authors: The Jackson Laboratory, Bar Harbor, ME, United States.
authors: John P
authors: JP
authors: Sundberg
authors: Department of Medicine, Medical University of South Carolina, Charleston, SC, United States; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, United States. Electronic address: awgulewa@musc.edu.
authors: Alexander
authors: A
authors: Awgulewitsch
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation / Q000235:genetics*; D006197:Hair / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012867:Skin / Q000378:metabolism / Q000473:pathology; D002785:Sterol O-Acyltransferase / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; C485834:Hoxc13 protein, mouse; D002785:Sterol O-Acyltransferase; C493492:sterol O-acyltransferase 1
keywords: Hair follicle; Hox regulatory target; Hoxc13; Lipid metabolism; Soat1