title: Domibacillus tundrae sp. nov., isolated from active layer soil of tussock tundra in Alaska, and emended description of the genus Domibacillus.
abstract: A novel Gram-stain-positive, spore-forming, aerobic, motile and rod-shaped bacterium designated strain PAMC 80007T was isolated from an active layer soil sample of Council, Alaska. Optimal growth of strain PAMC 80007T was observed at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain PAMC 80007T belonged to the genus Domibacillus. This strain was closely related to Domibacillus enclensis (98.3 %), Domibacillus robiginosus (98.3 %) and Domibacillus indicus (97.2 %). Genomic DNA G+C content was 43.5 mol% and genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 80007T is clearly distinguished from the closely related species of the genus Domibacillus. The major fatty acids (>5 %) were iso-C15 : 0 (24.7 %), C16 : 1ω11c (16.8 %), anteiso-C15 : 0 (16.5 %), C16 : 0 (15.6 %) and anteiso-C17 : 0 (8.7 %). The major respiratory isoprenoid quinones were menaquinone-6 (MK-6) and menaquinone-7 (MK-7), and the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, phospholipid and two unidentified lipids. meso-Diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan, and the major whole-cell sugar was ribose with a minor quantity of glucose. Results from a polyphasic study suggested that strain PAMC 80007T represents a novel species of the genus Domibacillus for which the name Domibacillus tundrae sp. nov. is proposed. The type strain is PAMC 80007T ( = JCM 30371T = KCTC 33549T = DSM 29572T). An emended description of the genus Domibacillus is also provided.
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Hye Ryeon
authors: HR
authors: Gyeong
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Kiwoon
authors: K
authors: Baek
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Chung Yeon
authors: CY
authors: Hwang
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Key Hun
authors: KH
authors: Park
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Hye Min
authors: HM
authors: Kim
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Hong Kum
authors: HK
authors: Lee
authors: Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
authors: Yoo Kyung
authors: YK
authors: Lee
mesh_terms: D000413:Alaska; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D065929:Tundra; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C042455:menaquinone 6; C062629:menaquinone 7