title: Up-regulation of Serum MiR-130b-3p Level is Associated with Renal Damage in Early Lupus Nephritis.
abstract: Systemic lupus erythematosus (SLE) is a common but severe autoimmune systemic inflammatory disease. Lupus nephritis (LN) is a serious complication of SLE,affecting up to 70% of SLE patients. Circulating microRNAs (miRNA) are emerging as biomarkers for pathological conditions and play significant roles in intercellular communication. In present research, serum samples from healthy control, early and late stage LN patients were used to analyze the expression profile of miRNAs by microarray. Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls. In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN. The further analysis found that the expression of serum miR-130b-3p was positively correlated with 24-hour proteinuria and renal chronicity index in patients with early stage LN.Transfection of renal tubular cellline(HK-2)with miR-130b-3p mimics can promote epithelial-mesenchymal transition (EMT). The opposite effects were observed when transfected with miR-130b-3p inhibitors. MiR-130b-3p negatively regulated ERBB2IP expression by directly targeting the 3'-UTR of ERBB2IP The circulating miR-130b-3p might serve as a biomarker and play an important role in renal damage in early stage LN patients.
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Wanpeng
authors: W
authors: Wang
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Shan
authors: S
authors: Mou
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Ling
authors: L
authors: Wang
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Minfang
authors: M
authors: Zhang
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Xinghua
authors: X
authors: Shao
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Wei
authors: W
authors: Fang
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Renhua
authors: R
authors: Lu
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Chaojun
authors: C
authors: Qi
authors: Department of Health Care center, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Zhuping
authors: Z
authors: Fan
authors: Department of Health Care center, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Qin
authors: Q
authors: Cao
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Qin
authors: Q
authors: Wang
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Yan
authors: Y
authors: Fang
authors: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
authors: Zhaohui
authors: Z
authors: Ni
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007668:Kidney / Q000473:pathology*; D007684:Kidney Tubules / Q000187:drug effects / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000235:genetics; D008181:Lupus Nephritis / Q000097:blood* / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C413364:ERBIN protein, human; C000597075:MIRN-1233 microRNA, human; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D053773:Transforming Growth Factor beta1