title: Effective cutaneous vaccination using an inactivated chikungunya virus vaccine delivered by Foroderm.
abstract: Foroderm is a new cutaneous delivery technology that uses high-aspect ratio, cylindrical silica microparticles, that are massaged into the skin using a 3D-printed microtextured applicator, in order to deliver payloads across the epidermis. Herein we show that this technology is effective for delivery of a non-adjuvanted, inactivated, whole-virus chikungunya virus vaccine in mice, with minimal post-vaccination skin reactions. A single topical Foroderm-based vaccination induced T cell, Th1 cytokine and antibody responses, which provided complete protection against viraemia and disease after challenge with chikungunya virus. Foroderm vaccination was shown to deliver fluorescent, virus-sized beads across the epidermis, with beads subsequently detected in draining lymph nodes. Foroderm vaccination also stimulated the egress of MHC II(+) antigen presenting cells from the skin. Foroderm thus has potential as a simple, cheap, effective, generic, needle-free technology for topical delivery of vaccines.
authors: Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane 4029, QLD, Australia.
authors: Penny A
authors: PA
authors: Rudd
authors: Dermatology Research Centre, University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, QLD, Australia.
authors: Anthony P
authors: AP
authors: Raphael
authors: Dermatology Research Centre, University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, QLD, Australia.
authors: Miko
authors: M
authors: Yamada
authors: Dermatology Research Centre, University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, QLD, Australia.
authors: Kaitlin L
authors: KL
authors: Nufer
authors: Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane 4029, QLD, Australia.
authors: Joy
authors: J
authors: Gardner
authors: Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane 4029, QLD, Australia.
authors: Thuy T T
authors: TT
authors: Le
authors: Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane 4029, QLD, Australia; Australian Infectious Disease Research Centre, School of Chemistry & Molecular Biosciences, University of Queensland, Brisbane 4072, QLD, Australia.
authors: Natalie A
authors: NA
authors: Prow
authors: Dermatology Research Centre, University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, QLD, Australia.
authors: Nhung
authors: N
authors: Dang
authors: Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane 4029, QLD, Australia.
authors: Wayne A
authors: WA
authors: Schroder
authors: Dermatology Research Centre, University of Queensland, School of Medicine, Translational Research Institute, Brisbane 4102, QLD, Australia. Electronic address: t.prow@uq.edu.au.
authors: Tarl W
authors: TW
authors: Prow
authors: Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane 4029, QLD, Australia; Australian Infectious Disease Research Centre, School of Chemistry & Molecular Biosciences, University of Queensland, Brisbane 4072, QLD, Australia.
authors: Andreas
authors: A
authors: Suhrbier
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D065632:Chikungunya Fever / Q000517:prevention & control*; D002646:Chikungunya virus / Q000276:immunology*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005260:Female; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000276:immunology; D016896:Treatment Outcome; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014766:Viremia / Q000517:prevention & control
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Chikungunya virus; Cutaneous vaccination; Foroderm; Vaccine delivery