title: Productive life cycle of adeno-associated virus serotype 2 in the complete absence of a conventional polyadenylation signal.
abstract: We showed that WT adeno-associated virus serotype 2 (AAV2) genome devoid of a conventional polyadenylation [poly(A)] signal underwent complete genome replication, encapsidation and progeny virion production in the presence of adenovirus. The infectivity of the progeny virion was also retained. Using recombinant AAV2 vectors devoid of a human growth hormone poly(A) signal, we also demonstrated that a subset of mRNA transcripts contained the inverted terminal repeat (ITR) sequence at the 3' end, which we designated ITR in RNA (ITRR). Furthermore, AAV replication (Rep) proteins were able to interact with the ITRR. Taken together, our studies suggest a new function of the AAV2 ITR as an RNA element to mediate transgene expression from poly(A)-deleted mRNA.
authors: Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.|Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: Lina
authors: L
authors: Wang
authors: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.|Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: Zifei
authors: Z
authors: Yin
authors: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.|Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: Yuan
authors: Y
authors: Wang
authors: Department of Orthopedics and Rehabilitation, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: Yuan
authors: Y
authors: Lu
authors: Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: Daniel
authors: D
authors: Zhang
authors: Shands Cancer Center, University of Florida College of Medicine, Gainesville, FL, 32611, USA.|Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Genetics Institute, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: Arun
authors: A
authors: Srivastava
authors: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.|E-Institute of TCM Internal Medicine, Shanghai Municipal Education Commission, Shanghai 201203, PR China.
authors: Changquan
authors: C
authors: Ling
authors: Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Genetics Institute, University of Florida College of Medicine, Gainesville, FL 32611, USA.
authors: George V
authors: GV
authors: Aslanidi
authors: Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32611, USA.|Shands Cancer Center, University of Florida College of Medicine, Gainesville, FL, 32611, USA.
authors: Chen
authors: C
authors: Ling
mesh_terms: D004261:DNA Replication; D000229:Dependovirus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D016679:Genome, Viral; D006801:Humans; D010322:Parvoviridae Infections / Q000821:virology; D026723:Polyadenylation; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020079:Terminal Repeat Sequences; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins