title: Polarization Transfer from Ligands Hyperpolarized by Dissolution Dynamic Nuclear Polarization for Screening in Drug Discovery.
abstract: Nuclear magnetic resonance (NMR) spectroscopy is a valuable technique for ligand screening, because it exhibits high specificity toward chemical structure and interactions. Dissolution dynamic nuclear polarization (DNP) is a recent advance in NMR methodology that enables the creation of non-equilibrium spin states, which can dramatically increase NMR sensitivity. Here, the transfer of such spin polarization from hyperpolarized ligand to protein is observed. Mixing hyperpolarized benzamidine with the serine protease trypsin, a "fingerprint" of enhanced protein signals is observed, which shows a different intensity profile than the equilibrium NMR spectrum of the protein, but coincides closely to the frequency profile of a saturation transfer difference (STD) NMR experiment. The DNP experiment benefits from hyperpolarization and enables observation of all frequencies in a single, rapid experiment. Based on these merits, it is an interesting alternative to the widely used STD experiment for identification of protein-ligand interactions.
authors: Chemistry Department, Texas A&M University, 3255 TAMU, College Station, TX 77843 (USA).
authors: Hlaing
authors: H
authors: Min
authors: Biochemistry Department, Texas A&M University, 2128 TAMU, College Station, TX 77843 (USA).|Current address: Biochemistry Department, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 (USA).
authors: Giridhar
authors: G
authors: Sekar
authors: Chemistry Department, Texas A&M University, 3255 TAMU, College Station, TX 77843 (USA). chilty@tamu.edu.
authors: Christian
authors: C
authors: Hilty
mesh_terms: D001550:Benzamidines / Q000737:chemistry; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism
chemical_list: D001550:Benzamidines; D008024:Ligands; D011506:Proteins; D014357:Trypsin; C032157:benzamidine
keywords: NMR spectroscopy; hyperpolarization; ligand binding; saturation transfer difference