title: Association of Nitric Oxide Synthase and Matrix Metalloprotease Single Nucleotide Polymorphisms with Preeclampsia and Its Complications.
abstract: BACKGROUND
  Preeclampsia is one of the leading causes of maternal and neonatal morbidity and mortality in the world, but its appearance is still unpredictable and its pathophysiology has not been entirely elucidated. Genetic studies have associated single nucleotide polymorphisms in genes encoding nitric oxide synthase and matrix metalloproteases with preeclampsia, but the results are largely inconclusive across different populations.
  
  
  OBJECTIVE
  To investigate the association of single nucleotide polymorphisms (SNPs) in NOS3 (G894T, T-786C, and a variable number of tandem repetitions VNTR in intron 4), MMP2 (C-1306T), and MMP9 (C-1562T) genes with preeclampsia in patients from Southeastern Brazil.
  
  
  METHODS
  This prospective case-control study enrolled 77 women with preeclampsia and 266 control pregnant women. Clinical data were collected to assess risk factors and the presence of severe complications, such as eclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.
  
  
  RESULTS
  We found a significant association between the single nucleotide polymorphism NOS3 T-786C and preeclampsia, independently from age, height, weight, or the other SNPs studied, and no association was found with the other polymorphisms. Age and history of preeclampsia were also identified as risk factors. The presence of at least one polymorphic allele for NOS3 T-786C was also associated with the occurrence of eclampsia or HELLP syndrome among preeclamptic women.
  
  
  CONCLUSIONS
  Our data support that the NOS3 T-786C SNP is associated with preeclampsia and the severity of its complications.
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Daniela P
authors: DP
authors: Leonardo
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Dulcinéia M
authors: DM
authors: Albuquerque
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Carolina
authors: C
authors: Lanaro
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Letícia C
authors: LC
authors: Baptista
authors: Department of Obstetrics and Gynaecology, School of Medicine, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: José G
authors: JG
authors: Cecatti
authors: Department of Obstetrics and Gynaecology, School of Medicine, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Fernanda G
authors: FG
authors: Surita
authors: Department of Obstetrics and Gynaecology, School of Medicine, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Mary A
authors: MA
authors: Parpinelli
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Fernando F
authors: FF
authors: Costa
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Carla F
authors: CF
authors: Franco-Penteado
authors: Hematology and Hemotherapy Center, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil; Department of Clinical Pathology, School of Medicine, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Kleber Y
authors: KY
authors: Fertrin
authors: Department of Obstetrics and Gynaecology, School of Medicine, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
authors: Maria Laura
authors: ML
authors: Costa
mesh_terms: D000328:Adult; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9