title: Microbial Selenium Nanoparticles (SeNPs) and Their Application as a Sensitive Hydrogen Peroxide Biosensor.
abstract: The cell-free extract, a crude enzyme (cytosolic and membrane fraction) obtained from an environmental isolate, Bacillus pumilus sp. BAB-3706 worked as excellent in reducing as well as stabilizing agent and facilitated the formation of stable colloidal selenium nanoparticles (SeNPs). Resulting nanoparticles were characterized using UV-vis spectrophotometer, TEM, EDAX, FT-IR and XRD, respectively. A working electrode was modified by coating the surface of indium tin oxide (ITO) with colloidal SeNPs. Successive additions of H2O2 (100 to 600 μM) in conventional three electrodes system, cyclic voltammeter with potential scan rate 25.0 mV/s, in 0.1 M phosphate buffer solution (PBS) yielded increase in current. A perpetual amperometric response at fixed potential (-1.0 V) and at selected time interval of 100 s showed different magnitude of current at every addition of H2O2. The linear range of detection of H2O2 was from 5 to 600 mM (R(2) = 0.9965), while the calculated limit of detection was found to be 3.00 μM. The current study suggested that microbial SeNPs can be used for fabrication of low cost, sensitive H2O2 biosensor.
authors: Department of Environmental Studies, Faculty of Science, The M. S. University of Baroda, Vadodara, 390002, Gujarat, India. suranjit@gmail.com.
authors: Kumar Suranjit
authors: KS
authors: Prasad
authors: Department of Chemistry, Sardar Patel University, Anand, 388120, Gujarat, India.
authors: Jayraj V
authors: JV
authors: Vaghasiya
authors: Department of Chemistry, Sardar Patel University, Anand, 388120, Gujarat, India.
authors: Saurabh S
authors: SS
authors: Soni
authors: ARIBAS, New Vallabh Vidyanagar, Anand, 388120, Gujarat, India.
authors: Jitesh
authors: J
authors: Patel
authors: ARIBAS, New Vallabh Vidyanagar, Anand, 388120, Gujarat, India.
authors: Rinkesh
authors: R
authors: Patel
authors: Department of Botany, B. R. A. Bihar University, Muzaffarpur, 842002, Bihar, India.
authors: Madhu
authors: M
authors: Kumari
authors: Gujarat Biodiversity Gene Bank, Udyogbhavan, Gandhinagar, Gujarat, 382010, India.
authors: Falguni
authors: F
authors: Jasmani
authors: National Chemical Laboratory, Pune, 411008, India.
authors: Kaliaperumal
authors: K
authors: Selvaraj
mesh_terms: D001407:Bacillus / Q000737:chemistry; D015374:Biosensing Techniques; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*
chemical_list: D006861:Hydrogen Peroxide; D012643:Selenium
keywords: Biosensor; EDAX; Hydrogen peroxide; Selenium nanoparticles; TEM; UV-vis spectroscopy