title: ROS production in homogenate from the body wall of sea cucumber Stichopus japonicus under UVA irradiation: ESR spin-trapping study.
abstract: Sea cucumber Stichopus japonicus (S. japonicus) shows a strong ability of autolysis, which leads to severe deterioration in sea cucumber quality during processing and storage. In this study, to further characterize the mechanism of sea cucumber autolysis, hydroxyl radical production induced by ultraviolet A (UVA) irradiation was investigated. Homogenate from the body wall of S. japonicas was prepared and subjected to UVA irradiation at room temperature. Electron Spin Resonance (ESR) spectra of the treated samples were subsequently recorded. The results showed that hydroxyl radicals (OH) became more abundant while the time of UVA treatment and the homogenate concentration were increased. Addition of superoxide dismutase (SOD), catalase, EDTA, desferal, NaN3 and D2O to the homogenate samples led to different degrees of inhibition on OH production. Metal cations and pH also showed different effects on OH production. These results indicated that OH was produced in the homogenate with a possible pathway as follows: O2(-) → H2O2 → OH, suggesting that OH might be a critical factor in UVA-induced S. japonicus autolysis.
authors: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China. Electronic address: qihang@dlpu.edu.cn.
authors: Hang
authors: H
authors: Qi
authors: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors: Xiu-fang
authors: XF
authors: Dong
authors: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors: Ya-ping
authors: YP
authors: Zhao
authors: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors: Nan
authors: N
authors: Li
authors: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors: Hui
authors: H
authors: Fu
authors: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China.
authors: Ding-ding
authors: DD
authors: Feng
authors: Technology Research Center, Liaoning Province Dalian Ocean Fisheries Group Company, Dalian 116000, PR China.
authors: Li
authors: L
authors: Liu
authors: Department of Agricultural and Biosystems Engineering, Iowa State University, IA 50011, USA.
authors: Chen-xu
authors: CX
authors: Yu
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005504:Food Analysis / Q000379:methods*; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000378:metabolism; D047528:Stichopus / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D002374:Catalase; D013482:Superoxide Dismutase
keywords: DMPO; Homogenate; Hydroxyl radicals; Sea cucumber Stichopus japonicus; UVA irradiation