title: Novel bimodal iron oxide particles for efficient tracking of human neural stem cells in vivo.
abstract: OBJECTIVE
  We validated novel bimodal iron oxide particles as substitute of ferumoxides for efficient labeling of human neural stem cells (NSCs). The dextrane-coated FeraTrack Direct (FTD)-Vio particles have additional far-red fluorophores for microscopic cell analysis.
  
  
  METHODS
  MR relaxometry, spectrophotometric iron determination and microscopy are used for characterization in vitro and in vivo.
  
  
  RESULTS
  Efficient uptake is not transfection agent-dependent. FTD-Vio594 labeling had no influence on viability, proliferation, migration and differentiation capacity. It allows MRI-based tracking of engrafted NSCs in mouse brain up to 11 days, complemented by bioluminescence imaging of firefly luciferase expressed by the engrafted cells.
  
  
  CONCLUSIONS
  Our results highlight the FTD-Vio594 particles as safe and sensitive substitute of ferumoxides for longitudinal tracking of NSCs in preclinical studies.
authors: In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Gleuelerstrasse 50, D-50931 Cologne, Germany.
authors: Markus
authors: M
authors: Aswendt
authors: In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Gleuelerstrasse 50, D-50931 Cologne, Germany.
authors: Nadine
authors: N
authors: Henn
authors: In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Gleuelerstrasse 50, D-50931 Cologne, Germany.
authors: Stefanie
authors: S
authors: Michalk
authors: In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Gleuelerstrasse 50, D-50931 Cologne, Germany.
authors: Gabriele
authors: G
authors: Schneider
authors: Miltenyi Biotec GmbH, Friedrich-Ebert-Strasse 68, 51429 Bergisch Gladbach, Germany.
authors: Mark-Steven
authors: MS
authors: Steiner
authors: Miltenyi Biotec GmbH, Friedrich-Ebert-Strasse 68, 51429 Bergisch Gladbach, Germany.
authors: Ursula
authors: U
authors: Bissa
authors: Miltenyi Biotec GmbH, Friedrich-Ebert-Strasse 68, 51429 Bergisch Gladbach, Germany.
authors: Christian
authors: C
authors: Dose
authors: In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Gleuelerstrasse 50, D-50931 Cologne, Germany.|Department of Radiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.|Percuros BV, Enschede, Drienerlolaan 5-Zuidhorst, 7522 NB Enschede, The Netherlands.
authors: Mathias
authors: M
authors: Hoehn
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide
keywords: MRI; bioluminescence imaging; cell tracking; iron oxide nanoparticle; neural stem cells