title: Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines.
abstract: Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells.
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Ji-An
authors: JA
authors: Yang
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Ji-Qiang
authors: JQ
authors: Li
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Ling-Min
authors: LM
authors: Shao
authors: Department of Nephrology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Qian
authors: Q
authors: Yang
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Bao-Hui
authors: BH
authors: Liu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Ting-Feng
authors: TF
authors: Wu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Peng
authors: P
authors: Wu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Wei
authors: W
authors: Yi
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors: Qian-Xue
authors: QX
authors: Chen
keywords: DNA damage; Glioblastoma; apoptosis; cell cycle; proliferation; puerarin