title: LBP/BPI homologue in Eisenia andrei earthworms.
abstract: LBP/BPIs are pattern recognition receptors that are often present in vertebrates and in invertebrates, and they play a defense role against pathogens. We have identified 1698 bp cDNA sequence from the Eisenia andrei earthworm with predicted amino acid sequence that shares homology with the LBP/BPI family (EaLBP/BPI). Sequence analysis of EaLBP/BPI proved the existence of two conserved domains with the potential ability to bind LPS. The predicted molecular mass of the EaLBP/BPI protein is 53.5 kDa, and its high basicity (pI 9.8) is caused by its high arginine content. Constitutive transcription of the Ealbp/bpi gene was shown in all tested tissues, with the highest level in coelomocytes and seminal vesicles; the lowest level was detected in the intestine. On the contrary, another earthworm LPS-binding molecule CCF (coelomic cytolytic factor) was expressed only in the intestine and coelomocytes. In E. andrei coelomocytes, the transcription of Ealbp/bpi gene was up-regulated in response to bacterial stimulation, reaching a maximum at 8 and 16 h post stimulation with Bacillus subtilis and Escherichia coli, respectively.
authors: Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, Prague 4, 142 20, Czech Republic.
authors: František
authors: F
authors: Škanta
authors: Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, Prague 4, 142 20, Czech Republic. Electronic address: kohler@biomed.cas.cz.
authors: Petra
authors: P
authors: Procházková
authors: Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, Prague 4, 142 20, Czech Republic.
authors: Radka
authors: R
authors: Roubalová
authors: Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, Prague 4, 142 20, Czech Republic.
authors: Jiří
authors: J
authors: Dvořák
authors: Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, Prague 4, 142 20, Czech Republic.
authors: Martin
authors: M
authors: Bilej
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; C021623:bactericidal permeability increasing protein
keywords: CCF; Earthworm; Eisenia; LBP/BPI; LPS; PRR