title: Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina.
abstract: Fecal contamination of water is a worrying problem because it is associated with the transmission of enteric pathogenic microorganisms that can cause many infectious diseases. In this study, an environmental survey was conducted to assess the level of viral contamination by viable enterovirus and rotavirus genome in two recreational rivers (Suquía and Xanaes) of Córdoba, Argentina. Quantitative microbial risk assessment (QMRA) was calculated to estimate the risk of rotavirus infection. Water sampling was carried out during a one-year period, the presence of total and fecal coliforms was determined and water samples were then concentrated for viral determination. Cell culture and indirect immunofluorescence were applied for enterovirus detection and RT-qPCR for rotavirus quantification. Coliform bacteria levels found in Suquía River often far exceeded the guideline limits for recreational waters. The Xanaes exhibited a lower level of bacterial contamination, frequently within the guideline limits. Enterovirus and rotavirus were frequently detected in the monitoring rivers (percentage of positive samples in Suquía: 78.6% enterovirus, 100% rotavirus; in Xanaes: 87.5% enterovirus, 18.7% rotavirus). Rotavirus was detected at a media concentration of 5.7×10(5) genome copies/L (gc/L) in the Suquía and 8.5×10(0)gc/L in the Xanaes. QMRA revealed high risk of rotavirus infection in the Suquía, at sampling points with acceptable and non-acceptable bacteria numbers. The Xanaes showed significantly lower health risk of rotavirus infection but it proved to be a public health hazard. The viral occurrence was not readily explained by the levels of bacteria indicators, thus viral monitoring should be included to determine microbiological water quality. These findings provide the first data of QMRA for recreational waters in Argentina and reveal the need for public awareness of the health implications of the use of the river waters.
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: V E
authors: VE
authors: Prez
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: P I
authors: PI
authors: Gil
authors: Laboratorio de Inmunología y Virología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina.
authors: C F
authors: CF
authors: Temprana
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: P R
authors: PR
authors: Cuadrado
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: L C
authors: LC
authors: Martínez
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: M O
authors: MO
authors: Giordano
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: G
authors: G
authors: Masachessi
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: M B
authors: MB
authors: Isa
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: V E
authors: VE
authors: Ré
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: J V
authors: JV
authors: Paván
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina.
authors: S V
authors: SV
authors: Nates
authors: Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n. Ciudad Universitaria, 5000 Córdoba, Argentina. Electronic address: patricia_barril@fcm.unc.edu.ar.
authors: P A
authors: PA
authors: Barril
mesh_terms: D001118:Argentina / Q000453:epidemiology; D004781:Environmental Exposure / Q000706:statistics & numerical data*; D045483:Rivers / Q000821:virology*; D012401:Rotavirus; D012400:Rotavirus Infections / Q000453:epidemiology*
keywords: Enterovirus; Environmental surveillance; Quantitative microbial risk assessment (QMRA); Rotavirus; Surface water; Water quality