title: Comparative Proteomics for the Evaluation of Protein Expression and Modifications in Neurodegenerative Diseases.
abstract: Together with hypothesis-driven approaches, high-throughput differential proteomic analysis performed primarily not only in human cerebrospinal fluid and serum but also on protein content of other tissues (blood cells, muscles, peripheral nerves, etc.) has been used in the last years to investigate neurodegenerative diseases. Even if the goal for these analyses was mainly the discovery of neurodegenerative disorders biomarkers, the characterization of specific posttranslational modifications (PTMs) and the differential protein expression resulted in being very informative to better define the pathological mechanisms. In this chapter are presented and discussed the positive aspects and challenges of the outcomes of some of our investigations on neurological and neurodegenerative disease, in order to highlight the important role of protein PTMs studies in proteomics-based approaches.
authors: Proteome Biochemistry, Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors: Antonio
authors: A
authors: Conti
authors: Proteome Biochemistry, Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Milano, Italy. Electronic address: m.alessio@hsr.it.
authors: Massimo
authors: M
authors: Alessio
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D009422:Nervous System Diseases / Q000378:metabolism; D019636:Neurodegenerative Diseases / Q000378:metabolism* / Q000503:physiopathology*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D040901:Proteomics
chemical_list: D015415:Biomarkers; D003598:Cytoskeletal Proteins; C484119:MYBPH protein, human; D002570:Ceruloplasmin
keywords: Amyotrophic lateral sclerosis; Biomarker; Cerebrospinal fluid; Mass spectrometry; Parkinson's disease; Posttranslational modifications; Two-dimensional electrophoresis