title: Lactobacillus vespulae sp. nov., isolated from gut of a queen wasp (Vespula vulgaris).
abstract: A Gram-stain-positive, oxidase- and catalase-negative, rod-shaped, facultatively anaerobic bacterial strain, DCY75T, was isolated from a queen wasp (Vespula vulgaris). Growth occurred at 4–37 °C (optimum, 30 °C), at pH 3.5–8.0 (optimum, pH 5.0–6.0) and with ≤ 7.0 % (w/v) NaCl. Strain DCY75T produced gas during growth on glucose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DCY75T belonged to the genus Lactobacillus and was closely related to Lactobacillus sanfranciscensis ATCC 27651T and Lactobacillus lindneri DSM 20690T at sequence similarities of 96.7 and 96.4 %, respectively. A comparison of two housekeeping genes, pheS and rpoA, revealed that strain DCT75T was well separated from other species of the genus Lactobacillus. Strain DCY75T produced d- and l-lactic acid isomers in a ratio of 22.5 : 77.5 (v/v). The major fatty acids were summed feature 8 (comprising C18 :  1ω7c and/or C18 : 1ω6c), C16 : 0, C18 : 1ω9c and C18 : 0.The peptidoglycan structure was of the A4α (l-Lys–d-Asp) type. Cell-wall sugars were glucose, galactose and ribose. The DNA G+C content was 35.5 ± 1.3 mol%. Based on phenotypic and genotypic properties, strain DCY75T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus vespulae sp. nov. is proposed. The type strain is DCY75T ( = KCTC 21023T = JCM 19742T).
authors: Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University Giheung-gu Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Van-An
authors: VA
authors: Hoang
authors: Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University Giheung-gu Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Yeon-Ju
authors: YJ
authors: Kim
authors: Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University Giheung-gu Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Ngoc-Lan
authors: NL
authors: Nguyen
authors: Department of Biomedical Chemistry, Institute of Biomedical & Health Science, College of Biomedical & Health Science, Konkuk University, Chungju-si 380-701, Republic of Korea.
authors: Si-Kwan
authors: SK
authors: Kim
authors: Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University Giheung-gu Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Deok-Chun
authors: DC
authors: Yang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014863:Wasps / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S