title: Comparison and development of pyrazinamide susceptibility testing methods for tuberculosis in Thailand.
abstract: Pyrazinamide (PZA) plays a critical role in shortening tuberculosis treatment duration and in treating multi-drug resistant tuberculosis (MDR-TB). The standard phenotypic MGIT PZA susceptibility testing method is imperfect because it is slow and has potential for false resistance. In this study, we evaluated 2 different phenotypic-based methods, quantitative real-time PCR (qPCR) phage assay, and MTT assay, as well as genotypic sequencing. The assay was evaluated on 71 clinical Mycobacterium tuberculosis isolates (37 MGIT PZA susceptible and 34 MGIT PZA resistant) and compared to the MGIT result. Of these methods, the qPCR phage assay yielded an accuracy of 89% versus standard MGIT while MTT yielded 83%. The genotypic sequencing method yielded 90% accuracy. We conclude that any of these faster PZA susceptibility methods perform reasonably well against a MGIT PZA susceptibility standard.
authors: Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors: Suporn
authors: S
authors: Foongladda
authors: Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors: Wiphat
authors: W
authors: Klayut
authors: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, VA, USA.
authors: Suporn
authors: S
authors: Pholwat
authors: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, VA, USA. Electronic address: erh6k@virginia.edu.
authors: Eric
authors: E
authors: Houpt
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011718:Pyrazinamide / Q000494:pharmacology*; D013785:Thailand; D013997:Time Factors
chemical_list: D000995:Antitubercular Agents; D011718:Pyrazinamide
keywords: Pyrazinamide; Susceptibility testing