title: Upregulation of miR-556-5p promoted prostate cancer cell proliferation by suppressing PPP2R2A expression.
abstract: The prognosis and survival rate of prostate cancer are very poor. Previous studies have shown that miR-556-5p have emerged as important regulators in cancer cell biological processes. The role of miR-556-5p in prostate cancer remains unclear. In this study, expression of miR-556-5p in prostate cancer cell lines and tissues was upregulated. Result of MTT assays, colony formation and anchorage-independent growth assays demonstrated that overexpression of miR-556-5p promoted prostate cancer cell growth. Additionally, PPP2R2A was identified as a direct target of miR-556-5p. Ectopic expression of miR-556-5p led to downregulation of PPP2R2A protein, which resulted in the downregulation of p27, upregulation of cyclin D1. Taken together, our data provide compelling evidence that miR-556-5p functions as an onco-miRNA and participates in prostate cancer carcinogenesis by suppressing PPP2R2A expression.
authors: Department of Urology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China; Department of Urology, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China.
authors: Wei
authors: W
authors: Zhao
authors: Department of Urology, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China.
authors: Longbin
authors: L
authors: Cao
authors: Central Laboratory, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China.
authors: Sha
authors: S
authors: Zeng
authors: Central Laboratory, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China.
authors: Haiping
authors: H
authors: Qin
authors: Department of Urology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China. Electronic address: tongyimen@126.com.
authors: Tongyi
authors: T
authors: Men
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D054648:Protein Phosphatase 2 / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: C530104:CCND1 protein, human; C436291:CDKN1B protein, human; C000611992:MIRN556 microRNA, human; D035683:MicroRNAs; C512974:PPP2R2A protein, human; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D054648:Protein Phosphatase 2
keywords: Cell proliferation; PPP2R2A; Prostate cancer; miR-556-5p