title: Direct fermentation route for the production of acrylic acid.
abstract: There have been growing concerns regarding the limited fossil resources and global climate changes resulting from modern civilization. Currently, finding renewable alternatives to conventional petrochemical processes has become one of the major focus areas of the global chemical industry sector. Since over 4.2 million tons of acrylic acid (AA) is annually employed for the manufacture of various products via petrochemical processes, this chemical has been the target of efforts to replace the petrochemical route by ecofriendly processes. However, there has been limited success in developing an approach combining the biological production of 3-hydroxypropionic acid (3-HP) and its chemical conversion to AA. Here, we report the first direct fermentative route for producing 0.12 g/L of AA from glucose via 3-HP, 3-HP-CoA, and Acryloyl-CoA, leading to a strain of Escherichia coli capable of directly producing acrylic acid. This route was developed through extensive screening of key enzymes and designing a novel metabolic pathway for AA.
authors: Biomaterials Lab, Material Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., 130 Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
authors: Hun Su
authors: HS
authors: Chu
authors: Biomaterials Lab, Material Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., 130 Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
authors: Jin-Ho
authors: JH
authors: Ahn
authors: Biomaterials Lab, Material Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., 130 Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
authors: Jiae
authors: J
authors: Yun
authors: Biomaterials Lab, Material Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., 130 Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
authors: In Suk
authors: IS
authors: Choi
authors: Biomaterials Lab, Material Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., 130 Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
authors: Tae-Wook
authors: TW
authors: Nam
authors: Biomaterials Lab, Material Research Center, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., 130 Samsung-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea. Electronic address: km3.cho@samsung.com.
authors: Kwang Myung
authors: KM
authors: Cho
mesh_terms: D000179:Acrylates / Q000378:metabolism*; D003065:Coenzyme A / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005285:Fermentation / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D055772:Green Chemistry Technology; D019344:Lactic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods; D053858:Metabolic Networks and Pathways / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D000179:Acrylates; D019344:Lactic Acid; C031601:hydracrylic acid; D005947:Glucose; C036658:acrylic acid; D003065:Coenzyme A
keywords: Acrylic acid; Direct fermentation; E. coli; Glucose