title: Magnetic resonance microscopy of renal and biliary abnormalities in excised tissues from a mouse model of autosomal recessive polycystic kidney disease.
abstract: Polycystic kidney disease (PKD) is transmitted as either an autosomal dominant or recessive trait and is a major cause of renal failure and liver fibrosis. The cpk mouse model of autosomal recessive PKD (ARPKD) has been extensively characterized using standard histopathological techniques after euthanasia. In the current study, we sought to validate magnetic resonance microscopy (MRM) as a robust tool for assessing the ARPKD phenotype. We used MRM to evaluate the liver and kidney of wild-type and cpk animals at resolutions <100 μm and generated three-dimensional (3D) renderings for pathological evaluation. Our study demonstrates that MRM is an excellent method for evaluating the complex, 3D structural defects in this ARPKD mouse model. We found that MRM was equivalent to water displacement in assessing kidney volume. Additionally, using MRM we demonstrated for the first time that the cpk liver exhibits less extensive ductal arborization, that it was reduced in volume, and that the ductal volume was disproportionately smaller. Histopathology indicates that this is a consequence of bile duct malformation. With its reduced processing time, volumetric information, and 3D capabilities, MRM will be a useful tool for future in vivo and longitudinal studies of disease progression in ARPKD. In addition, MRM will provide a unique tool to determine whether the human disease shares the newly appreciated features of the murine biliary phenotype.
authors: Department of Neuroscience, University of Florida, Gainesville, Florida McKnight Brain Institute, University of Florida, Gainesville, Florida.
authors: Choong H
authors: CH
authors: Lee
authors: Center for Translational Science, Children's National Health System, Washington, District of Columbia.
authors: Amber K
authors: AK
authors: O'Connor
authors: Center for Translational Science, Children's National Health System, Washington, District of Columbia.
authors: Chaozhe
authors: C
authors: Yang
authors: Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama.
authors: Joshua M
authors: JM
authors: Tate
authors: Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama.
authors: Trenton R
authors: TR
authors: Schoeb
authors: Department of Neuroscience, University of Florida, Gainesville, Florida McKnight Brain Institute, University of Florida, Gainesville, Florida.
authors: Jeremy J
authors: JJ
authors: Flint
authors: Department of Neuroscience, University of Florida, Gainesville, Florida McKnight Brain Institute, University of Florida, Gainesville, Florida National High Magnetic Field Laboratory, Tallahassee, Florida.
authors: Stephen J
authors: SJ
authors: Blackband
authors: Center for Translational Science, Children's National Health System, Washington, District of Columbia LGuaywoo@childrensnational.org.
authors: Lisa M
authors: LM
authors: Guay-Woodford
keywords: Autosomal recessive polycystic kidney disease; cpk mouse; magnetic resonance microscopy; polycystic kidney disease