title: Determination of the Relationship Between rs4986790 and rs4986791 Variants of TLR4 Gene and Lung Cancer.
abstract: Chronic inflammation triggers DNA damage and oncogenic mutations and causes tumor formation and tumor progression. One of the important components of the inflammatory response is Toll-like receptor (TLR) family. The objective of our study is to determine the relationship between rs4986790(+896A/G) and rs4986791(+1196C/T) gene polymorphisms and lung cancer risk. PCR-RFLP technique was carried out to identify the genotypes in 100 control individuals and 160 lung cancer patients. DNA extracted from peripheral blood samples were amplified and digested with NcoI and HinfI then visualized. We did not find any difference between genotype frequencies between controls and patients (p > 0.05) in rs4986790. But a significant difference between control group and patients with lung cancer as for genotype frequencies (χ (2) = 4.19, p = 0.041) in rs4986791 variants was found. Our data indicate that any correlation was not found between rs4986790 polymorphism and lung cancer, while a correlation between rs4986791 and lung cancer has been determined and found to be associated with lung cancer risk.
authors: Department of Medical Biology, Eskisehir Osmangazi University Medical Faculty, Buyukdere Mh., Meselik Kampusu, 26040, Eskisehir, Turkey. hkurtayda@gmail.com.
authors: Hulyam
authors: H
authors: Kurt
authors: Department of Medical Biology, Eskisehir Osmangazi University Medical Faculty, Buyukdere Mh., Meselik Kampusu, 26040, Eskisehir, Turkey.
authors: Cansu
authors: C
authors: Ozbayer
authors: Department of Biology, Artvin Coruh University, Faculty of Arts and Sciences, Artvin, Turkey.
authors: Aysegul
authors: A
authors: Bayramoglu
authors: Department of Medical Biology, Eskisehir Osmangazi University Medical Faculty, Buyukdere Mh., Meselik Kampusu, 26040, Eskisehir, Turkey.
authors: Hasan Veysi
authors: HV
authors: Gunes
authors: Department of Medical Biology, Eskisehir Osmangazi University Medical Faculty, Buyukdere Mh., Meselik Kampusu, 26040, Eskisehir, Turkey.
authors: İrfan
authors: İ
authors: Degirmenci
authors: Department of Biostatistic, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey.
authors: Kevser Setenay
authors: KS
authors: Oner
authors: Department of Chest Diseases, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey.
authors: Muzaffer
authors: M
authors: Metintas
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000139:chemically induced; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012906:Smoke / Q000009:adverse effects; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014421:Turkey
chemical_list: D012906:Smoke; D051197:Toll-Like Receptor 4
keywords: lung cancer; polymorphism; rs4986790; rs4986791; toll-like receptor-4