title: PI3K is involved in P2Y receptor-regulated cAMP /Epac/Kv channel signaling pathway in pancreatic β cells.
abstract: P2Y receptors (P2YR) are a family of purinergic G protein-coupled receptors, which could be stimulated by extracellular nucleotides. In pancreatic β cells, activation of P2YR has long been shown to stimulate insulin secretion in a glucose-dependent manner. Previously, we reported that P2YR-modulated insulin secretion is mediated by a cAMP/Epac/Kv channel pathway. However, the interaction between Epac and the Kv channel in P2YR-modulated insulin secretion remains unclear. In this study, we used patch-clamp technique and insulin secretion assay to investigate the potential molecules that may link Epac to Kv channel inhibition induced by P2YR activation. We identified that phosphatidylinositide 3-kinase, which mediates P2YR-regulated insulin secretion, is a critical mediator between Epac and the Kv channel.
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: yizhang313@163.com.
authors: Yi
authors: Y
authors: Zhang
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: 1074684265@qq.com.
authors: Hui
authors: H
authors: Wang
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: gqluckyy@163.com.
authors: Qing
authors: Q
authors: Guo
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: 18734893498@163.com.
authors: Xiaodong
authors: X
authors: Li
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: 13633513158@163.com.
authors: Jingying
authors: J
authors: Gao
authors: Department of Endocrinology, The First Hospital of Shanxi Medical University, Shanxi Medical University, Taiyuan, China. Electronic address: nectarliu@163.com.
authors: Yunfeng
authors: Y
authors: Liu
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: tyyangxiaoluo@163.com.
authors: Caihong
authors: C
authors: Yang
authors: Department of Pharmacology, Shanxi Medical University, Taiyuan, China. Electronic address: nlgite@outlook.com.
authors: Longgang
authors: L
authors: Niu
authors: Department of Endocrinology, The First Hospital of Shanxi Medical University, Shanxi Medical University, Taiyuan, China. Electronic address: yangjlm@126.com.
authors: Jing
authors: J
authors: Yang
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D018408:Patch-Clamp Techniques; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D058470:Receptors, Purinergic P2Y / Q000378:metabolism*; D015398:Signal Transduction; D018631:ras Proteins / Q000378:metabolism
chemical_list: D000242:Cyclic AMP; D020662:Guanine Nucleotide Exchange Factors; D007328:Insulin; D019869:Phosphatidylinositol 3-Kinases; D024642:Potassium Channels, Voltage-Gated; D058470:Receptors, Purinergic P2Y; D018631:ras Proteins; C526973:Rapgef3 protein, rat
keywords: Epac; Insulin secretion; P2Y purinergic receptor; Phosphatidylinositide 3-kinase; Voltage-dependent potassium channels