title: A comprehensive model to predict mitotic division in budding yeasts.
abstract: High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division.
authors: Department of Solid State Physics, Indian Association for the Cultivation of Science, Kolkata 700032, India.
authors: Sabyasachi
authors: S
authors: Sutradhar
authors: Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India.
authors: Vikas
authors: V
authors: Yadav
authors: Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India.
authors: Shreyas
authors: S
authors: Sridhar
authors: Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India.
authors: Lakshmi
authors: L
authors: Sreekumar
authors: Tata Memorial Centre, Advanced Centre for Treatment Research and Education in Cancer, Kharghar, Navi Mumbai 410210, India.
authors: Dibyendu
authors: D
authors: Bhattacharyya
authors: Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400076, India.
authors: Santanu Kumar
authors: SK
authors: Ghosh
authors: Department of Solid State Physics, Indian Association for the Cultivation of Science, Kolkata 700032, India sanyal@jncasr.ac.in ssprp@iacs.res.in.
authors: Raja
authors: R
authors: Paul
authors: Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India sanyal@jncasr.ac.in ssprp@iacs.res.in.
authors: Kaustuv
authors: K
authors: Sanyal
mesh_terms: D020090:Chromosome Segregation; D003593:Cytoplasm / Q000378:metabolism; D056445:Cytoplasmic Dyneins / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D008954:Models, Biological; D004718:Saccharomycetales / Q000166:cytology* / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D056445:Cytoplasmic Dyneins; D004398:Dyneins