title: Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana.
abstract: The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25±7.8 nM) is favored relative to homodimerization (431±59 nM). Interaction of Lil3.2 with chlorophyll a (231±49 nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thaliana.
authors: Center for Organelle Research, University of Stavanger, Stavanger, Norway.
authors: Astrid Elisabeth
authors: AE
authors: Mork-Jansson
authors: Center for Organelle Research, University of Stavanger, Stavanger, Norway.
authors: Daniela
authors: D
authors: Gargano
authors: Center for Organelle Research, University of Stavanger, Stavanger, Norway.
authors: Karol
authors: K
authors: Kmiec
authors: Center for Organelle Research, University of Stavanger, Stavanger, Norway.
authors: Clemens
authors: C
authors: Furnes
authors: Center for Organelle Research, University of Stavanger, Stavanger, Norway; Department of Chemistry, Chemical Biological Centre (KBC), Umeå University, Sweden.
authors: Dmitriy
authors: D
authors: Shevela
authors: Center for Organelle Research, University of Stavanger, Stavanger, Norway. Electronic address: lutz.eichacker@uis.no.
authors: Lutz Andreas
authors: LA
authors: Eichacker
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D002734:Chlorophyll / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D055503:Protein Multimerization; D020349:Surface Plasmon Resonance
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D045342:Light-Harvesting Protein Complexes; C587631:light-harvesting-like protein 3, Arabidopsis; D002734:Chlorophyll
keywords: Chlorophyll a; Hydrophobic cluster analysis plot; Light harvesting like protein 3; Microscale thermophoresis