title: Histopathologic and Cytogenetic Features of Pulmonary Adenoid Cystic Carcinoma.
abstract: BACKGROUND
  A significant portion of adenoid cystic carcinoma (ACC) cases are characterized by a t(6;9)(q22-23;p23-24) translocation that originates a MYB-NFIB fusion oncogene. The MYB-NFIB fusion oncoprotein activates transcription of MYB-mediated pathways that impact cell cycle control, DNA repair, and apoptosis. This translocation seems highly specific for ACC. Moreover, therapies targeting MYB-activated pathways to treat ACC are being explored. Pulmonary ACC (PACC) has not been thoroughly studied for rearrangements of the MYB gene.
  
  
  METHODS
  Mayo Clinic Rochester surgical pathology archives (1972-2011) were searched for PACC. All cases were reviewed and classified according to the predominant histologic pattern (cribriform, solid, and tubular) by two surgical pathologists. Fluorescence in situ hybridization (FISH) was employed using a break-apart strategy to detect MYB rearrangement (at 6q23.3). Medical records were studied.
  
  
  RESULTS
  Forty cases of PACC were studied; tissue blocks were available for FISH analysis in 35 cases. Six cases failed to hybridize. In 12 of 29 cases (41%), the MYB gene region was disrupted, whereas 17 cases (59%) showed no evidence of rearrangement. FISH studies performed on other histologic subtypes of lung cancer (10 squamous cell carcinomas, 10 adenocarcinomas, and 10 small-cell carcinomas) failed to show MYB rearrangement. There was no significant difference in MYB rearrangement status with respect to predominant histologic pattern, clinical features, or clinical outcome.
  
  
  CONCLUSIONS
  A MYB rearrangement was identified in 41% of PACC and was 100% specific. FISH studies for MYB may be of diagnostic utility in PACC, particularly on small biopsy specimens. MYB rearrangement in PACC does not seem to be associated with clinical features or prognosis.
authors: *Department of Laboratory Medicine and Pathology, †Medical Genome Facility, Cytogenetics Core Laboratory, ‡Department of Health Sciences Research, and §Division of Medical Oncology, Mayo Clinic Rochester, Minnesota.
authors: Anja C
authors: AC
authors: Roden
authors: Patricia T
authors: PT
authors: Greipp
authors: Darlene L
authors: DL
authors: Knutson
authors: Sara M
authors: SM
authors: Kloft-Nelson
authors: Sarah M
authors: SM
authors: Jenkins
authors: Randolph S
authors: RS
authors: Marks
authors: Marie Christine
authors: MC
authors: Aubry
authors: Joaquín J
authors: JJ
authors: García
mesh_terms: D000328:Adult; D000368:Aged; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D003582:Cytogenetics / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D055815:Young Adult
chemical_list: D015514:Oncogene Proteins, Fusion