title: Binding of CXCL8/IL-8 to Mycobacterium tuberculosis Modulates the Innate Immune Response.
abstract: Interleukin-8 (IL-8) has been implicated in the pathogenesis of several human respiratory diseases, including tuberculosis (TB). Importantly and in direct relevance to the objectives of this report quite a few findings suggest that the presence of IL-8 may be beneficial for the host. IL-8 may aid with mounting an adequate response during infection with Mycobacterium tuberculosis (M. tb); however, the underlying mechanism remains largely unknown. The major goal of our study was to investigate the contribution of IL-8 to the inflammatory processes that are typically elicited in patients with TB. We have shown for the first time that IL-8 can directly bind to tubercle bacilli. We have also demonstrated that association of IL-8 with M. tb molecules leads to the augmentation of the ability of leukocytes (neutrophils and macrophages) to phagocyte and kill these bacilli. In addition, we have shown that significant amount of IL-8 present in the blood of TB patients associates with erythrocytes. Finally, we have noted that IL-8 is the major chemokine responsible for recruiting T lymphocytes (CD3(+), CD4(+), and CD8(+) T cells). In summary, our data suggest that the association of IL-8 with M. tb molecules may modify and possibly enhance the innate immune response in patients with TB.
authors: Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA ; Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.
authors: Agnieszka
authors: A
authors: Krupa
authors: Department of Immunology and Infectious Biology, University of Lodz, 90-237 Lodz, Poland.
authors: Marek
authors: M
authors: Fol
authors: Department of Immunoparasitology, University of Lodz, 90-237 Lodz, Poland.
authors: Bozena R
authors: BR
authors: Dziadek
authors: Department of Immunology and Infectious Biology, University of Lodz, 90-237 Lodz, Poland.
authors: Ewa
authors: E
authors: Kepka
authors: Department of Immunology and Infectious Biology, University of Lodz, 90-237 Lodz, Poland.
authors: Dominika
authors: D
authors: Wojciechowska
authors: Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.
authors: Anna
authors: A
authors: Brzostek
authors: Department of Immunobiology of Bacteria, University of Lodz, 90-237 Lodz, Poland.
authors: Agnieszka
authors: A
authors: Torzewska
authors: Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.
authors: Jaroslaw
authors: J
authors: Dziadek
authors: Department of Medicine, University of Cincinnati Medical Center, Cincinnati, OH 45219, USA.
authors: Robert P
authors: RP
authors: Baughman
authors: Department of Medicine, University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA.
authors: David
authors: D
authors: Griffith
authors: Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA.
authors: Anna K
authors: AK
authors: Kurdowska
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D016209:Interleukin-8 / Q000032:analysis / Q000502:physiology*; D008168:Lung / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010587:Phagocytosis
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8