title: Formation of Organized Protein Thin Films with External Electric Field.
abstract: The effect of an external electric field on the formation of protein GlnB-Hs films and on its buffer solution on siliconized glass slides has been analyzed by current versus electric field curves and atomic force microscopy (AFM). The Herbaspirillum seropedicae GlnB protein (GlnB-Hs) is a globular, soluble homotrimer (36 kDa) with its 3-D structure previously determined. Concentrations of 10 nM native denatured GlnB-Hs protein were deposited on siliconized glass slides under ambient conditions. Immediately after solution deposition a maximum electric field of 30 kV/m was applied with rates of 3 V/s. The measured currents were surface currents and were analyzed as transport current. Electric current started to flow only after a minimum electric field (critical value) for the systems analyzed. The AFM images showed films with a high degree of directional organization only when the proteins were present in the solution. These results showed that the applied electric field favored directional organization of the protein GlnB-Hs films and may contribute to understand the formation of protein films under applied electric fields.
authors: PIPE, Federal University of Paraná , Centro Politécnico - Adm. Building, 2° Floor, 81531-990 Curitiba, Paraná, Brazil.|Biochemistry and Molecular Biology, Federal University of Paraná , P.O. Box 19046, 81531-990 Curitiba, Paraná, Brazil.
authors: Cecília Fabiana da G
authors: CF
authors: Ferreira
authors: PIPE, Federal University of Paraná , Centro Politécnico - Adm. Building, 2° Floor, 81531-990 Curitiba, Paraná, Brazil.|Biochemistry and Molecular Biology, Federal University of Paraná , P.O. Box 19046, 81531-990 Curitiba, Paraná, Brazil.
authors: Paulo C
authors: PC
authors: Camargo
authors: PIPE, Federal University of Paraná , Centro Politécnico - Adm. Building, 2° Floor, 81531-990 Curitiba, Paraná, Brazil.|Biochemistry and Molecular Biology, Federal University of Paraná , P.O. Box 19046, 81531-990 Curitiba, Paraná, Brazil.
authors: Elaine M
authors: EM
authors: Benelli
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D004560:Electricity; D042803:Herbaspirillum / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D051922:PII Nitrogen Regulatory Proteins / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D051922:PII Nitrogen Regulatory Proteins; D012828:Silicones; C404143:PIID regulatory protein, Bacteria