title: Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface-Enhanced Raman Scattering.
abstract: Hydrogen sulfide (H2 S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2 S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2 S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2 S. Furthermore, AuNPs/4-AA responds to H2 S within 1 min with a 0.1 μM level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2 S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2 S.
authors: Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China). ytlong@ecust.edu.cn.
authors: Da-Wei
authors: DW
authors: Li
authors: Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China).
authors: Lu-Lu
authors: LL
authors: Qu
authors: Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China).
authors: Kai
authors: K
authors: Hu
authors: Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China). daweili@ecust.edu.cn.
authors: Yi-Tao
authors: YT
authors: Long
authors: Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China).
authors: He
authors: H
authors: Tian
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry; D051381:Rats; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D001386:Azides; D006046:Gold; D006862:Hydrogen Sulfide
keywords: hydrogen sulfide; living cells; monitoring; nanosensors; surface-enhanced Raman spectroscopy