title: Isolation of Tahyna Virus (California Encephalitis Group) From Anopheles hyrcanus (Diptera, Culicidae), a Mosquito Species New to, and Expanding in, Central Europe.
abstract: Two strains of Tahyna virus (TAHV; Orthobunyavirus, Bunyaviridae) were isolated from 4,568 (92 pools) female Anopheles hyrcanus Pallas (Diptera, Culicidae) mosquitoes collected on the fishponds in South Moravia (Czechland, central Europe) during July-August 2013. This is the first isolation of TAHV from An. hyrcanus in Europe. An. hyrcanus is a species new to Czechland since 2007; its population density was very high in the year 2013 at these ponds. The virus isolation procedure was based on intracerebral inoculation of newborn mice; moreover, the positive pools were also tested by polymerase chain reaction and found to contain TAHV RNA. An. hyrcanus, feeding preferentially on mammals including humans, may be a new potential vector for TAHV in Europe.
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic zhubalek@brno.cas.cz.
authors: Z
authors: Z
authors: Hubalek
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic.
authors: O
authors: O
authors: Sebesta
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic.
authors: J
authors: J
authors: Pesko
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic.
authors: L
authors: L
authors: Betasova
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic.
authors: H
authors: H
authors: Blazejova
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic.
authors: K
authors: K
authors: Venclikova
authors: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., CZ-60365 Brno, Czech Republic.
authors: I
authors: I
authors: Rudolf
mesh_terms: D000818:Animals; D000852:Anopheles / Q000821:virology*; D018153:Czech Republic; D002141:Encephalitis Virus, California / Q000302:isolation & purification*; D005260:Female; D051379:Mice; D000072138:Mosquito Vectors / Q000821:virology*
keywords: Anopheles hyrcanus; Orthobunyavirus; Tahyna virus; mosquito