title: Nano-mechanical model of endothelial dysfunction for AFM-based diagnostics at the cellular level.
abstract: A review of recent experimental investigations on the nanomechanical response of individual endothelial cells to inflammation caused by environmental agents and selected chemical compounds is presented. We focus on the results obtained by means of the force spectroscopy using the tip of an atomic force microscope as an imaging and nanoindentation spectroscopic probe. The findings presented in this review allow validating the nanoindentation method as a tool for quantitative cell elasticity probing and thereby allow proposing a nanomechanical model of endothelial dysfunction that could be practically used for drug efficacy and toxicity profiling in the endothelium at the subcellular level.
authors: Research Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Advanced Computer Science, Jagiellonian University, Kraków, Poland. Electronic address: ufszymon@cyf-kr.edu.pl.
authors: Marek
authors: M
authors: Szymonski
authors: Research Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Advanced Computer Science, Jagiellonian University, Kraków, Poland.
authors: Marta
authors: M
authors: Targosz-Korecka
authors: Research Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Advanced Computer Science, Jagiellonian University, Kraków, Poland.
authors: Katarzyna E
authors: KE
authors: Malek-Zietek
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000473:pathology / Q000648:ultrastructure; D048429:Cell Size; D004730:Endothelium, Vascular / Q000166:cytology* / Q000473:pathology*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*
keywords: Atomic force microscopy; Cellular nanomechanics; Endothelial dysfunction; Endothelium cells; Nanoindentation