title: Endosialin‑expressing bone sarcoma stem‑like cells are highly tumor‑initiating and invasive.
abstract: It has been reported that the presence of a small group of cancer stem‑like 'side population (SP)' cells is responsible for therapy failure and tumor recurrence. The present study demonstrated that primary human osteosarcoma samples contained a SP of about 3.9% which overexpressed ABC transporters, including ABCA1, ABCB1, ABCB2 and ABCG2, which are associated with drug resistance and may have contributed to multi‑drug resistance of SP cells. Furthermore, these SP cells displayed increased expression of endosialin (CD248) and other stem cell surface proteins, including CD133, octamer‑binding transcription factor 3/4A, Nanog and Nestin, which are ultimately responsible for high self‑renewal and deregulated cell proliferation. In addition, it was shown that endosialin‑overexpressing SP cells were able to regenerate the tumor population and had a high invasive potential. Therefore, the present study suggested that osteosarcoma SP cells were cancer stem cells, as they displayed stem‑like properties; furthermore, endosialin may be a potential target to prevent osteosarcoma recurrence following chemotherapy.
authors: Department of Operating Room, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors: Dong-Xiu
authors: DX
authors: Sun
authors: Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors: Guang-Jun
authors: GJ
authors: Liao
authors: The Joint Department of Orthopaedics, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors: Ke-Gui
authors: KG
authors: Liu
authors: Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors: Han
authors: H
authors: Jian
mesh_terms: D000071916:AC133 Antigen; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000328:Adult; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D001859:Bone Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology / Q000601:surgery; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D000071317:Nanog Homeobox Protein; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012516:Osteosarcoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology / Q000601:surgery; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D058985:Side-Population Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction
chemical_list: D000071916:AC133 Antigen; D018528:ATP-Binding Cassette Transporters; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; C078203:CD248 protein, human; D006023:Glycoproteins; D018398:Homeodomain Proteins; C486780:NANOG protein, human; C577484:NES protein, human; D000071317:Nanog Homeobox Protein; D064231:Nestin; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C000606023:PROM1 protein, human; D010455:Peptides