title: GATA-1 directly regulates Nanog in mouse embryonic stem cells.
abstract: Nanog safeguards pluripotency in mouse embryonic stem cells (mESCs). Insight into the regulation of Nanog is important for a better understanding of the molecular mechanisms that control pluripotency of mESCs. In a silico analysis, we identify four GATA-1 putative binding sites in Nanog proximal promoter. The Nanog promoter activity can be significantly repressed by ectopic expression of GATA-1 evidenced by a promoter reporter assay. Mutation studies reveal that one of the four putative binding sites counts for GATA-1 repressing Nanog promoter activity. Direct binding of GATA-1 on Nanog proximal promoter is confirmed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression.
authors: College of Life Sciences, Northwest A&F University, Yangling 712100, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, PR China.
authors: Wen-Zhong
authors: WZ
authors: Li
authors: College of Life Sciences, Northwest A&F University, Yangling 712100, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, PR China.
authors: Zhi-Ying
authors: ZY
authors: Ai
authors: School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
authors: Zhi-Wei
authors: ZW
authors: Wang
authors: College of Life Sciences, Northwest A&F University, Yangling 712100, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, PR China.
authors: Lin-Lin
authors: LL
authors: Chen
authors: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, PR China. Electronic address: gzknwaf@126.com.
authors: Ze-Kun
authors: ZK
authors: Guo
authors: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, PR China. Electronic address: zylabnwaf@126.com.
authors: Yong
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells; D000071317:Nanog Homeobox Protein; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse
keywords: GATA-1; Nanog; Nanog proximal promoter