title: Highly sensitive amperometric Pt-Nafion gas phase nitric oxide sensor: Performance and application in characterizing nitric oxide-releasing biomaterials.
abstract: A highly sensitive amperometric gas-phase nitric oxide (NO) sensor based on a Pt working electrode chemically deposited on a Nafion film is described. The Pt electrode is chemically deposited on a Nafion 117 membrane by impregnating the film with Pt(NH3)4(2+) ions, which are then exposed to NaBH4 to precipitate conductive Pt metal. The sensor was characterized with a mass-flow controlled 1 ppm NO standard gas and has an electrochemical surface area of 34 ± 9 cm(2), low limits of detection (4.3 ± 1.1 ppb) and a fast response time (<5s) toward changes in gas phase NO levels. Good correlation was found for measurements with the new amperometric Pt-Nafion sensor vs. chemiluminescence results for detecting the rates of NO release from Carbosil2080A polymer films doped with S-nitroso-N-acetylpenicillamine (R = 0.999, m = 0.999, n = 6) and for electrochemical reduction of nitrite to NO (R = 0.999, m = 0.938, n = 3) mediated by a copper(II)-tri(2-pyridylmethyl)amine complex.
authors: Department of Chemistry, 930 N. University Avenue, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Zheng
authors: Z
authors: Zheng
authors: Department of Chemistry, 930 N. University Avenue, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Hang
authors: H
authors: Ren
authors: Department of Chemistry, 930 N. University Avenue, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Ian
authors: I
authors: VonWald
authors: Department of Chemistry, 930 N. University Avenue, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mmeyerho@umich.edu.
authors: Mark E
authors: ME
authors: Meyerhoff
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004573:Electrolytes / Q000737:chemistry; D004867:Equipment Design; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000032:analysis*; D020030:Nitric Oxide Donors / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D026423:S-Nitroso-N-Acetylpenicillamine / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D005465:Fluorocarbon Polymers; D020030:Nitric Oxide Donors; D011108:Polymers; D009569:Nitric Oxide; C040402:perfluorosulfonic acid; D010984:Platinum; D026423:S-Nitroso-N-Acetylpenicillamine
keywords: Amperometric gas-phase sensor; Electrochemical nitric oxide generation; Nafion; Nitric oxide; S-nitrosothiol