title: High-throughput methods for identification of protein-protein interactions involving short linear motifs.
abstract: Interactions between modular domains and short linear motifs (3-10 amino acids peptide stretches) are crucial for cell signaling. The motifs typically reside in the disordered regions of the proteome and the interactions are often transient, allowing for rapid changes in response to changing stimuli. The properties that make domain-motif interactions suitable for cell signaling also make them difficult to capture experimentally and they are therefore largely underrepresented in the known protein-protein interaction networks. Most of the knowledge on domain-motif interactions is derived from low-throughput studies, although there exist dedicated high-throughput methods for the identification of domain-motif interactions. The methods include arrays of peptides or proteins, display of peptides on phage or yeast, and yeast-two-hybrid experiments. We here provide a survey of scalable methods for domain-motif interaction profiling. These methods have frequently been applied to a limited number of ubiquitous domain families. It is now time to apply them to a broader set of peptide binding proteins, to provide a comprehensive picture of the linear motifs in the human proteome and to link them to their potential binding partners. Despite the plethora of methods, it is still a challenge for most approaches to identify interactions that rely on post-translational modification or context dependent or conditional interactions, suggesting directions for further method development.
authors: Department of Chemistry - BMC, Husargatan 3, 751 23, Uppsala, Sweden.
authors: Cecilia
authors: C
authors: Blikstad
authors: Department of Chemistry - BMC, Husargatan 3, 751 23, Uppsala, Sweden. Ylva.ivarsson@kemi.uu.se.
authors: Ylva
authors: Y
authors: Ivarsson
mesh_terms: D020816:Amino Acid Motifs; D057166:High-Throughput Screening Assays / Q000191:economics / Q000379:methods; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D040081:Protein Array Analysis / Q000191:economics / Q000379:methods; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000191:economics / Q000379:methods*; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000191:economics / Q000379:methods; D020798:Two-Hybrid System Techniques
chemical_list: D019151:Peptide Library; D010455:Peptides; D011506:Proteins