title: Inflammation neither increases hepatic hepcidin nor affects intestinal (59)Fe-absorption in two murine models of bowel inflammation, hemizygous TNF(ΔARE/+) and homozygous IL-10(-/-) mice.
abstract: Hepcidin-synthesis was reported to be stimulated by inflammation. In contrast, hepcidin synthesis was inhibited by TNFα and serum hepcidin was low. To elucidate these contradictions, we compare data on hepcidin expression, on iron absorption and homoeostasis and markers of inflammation between two murine models of intestinal inflammation and corresponding wild-types as determined by standard methods. In TNF(ΔARE/+) and IL-10(-/-)-mice hepatic hepcidin expression and protein content was significantly lower than in corresponding wild-types. However, (59)Fe whole-body retention showed no difference between knock-outs and corresponding wild-types 7d after gavage, in neither strain. Compared to wild-types, body weight, hepatic non-haem iron content, hemoglobin and hematocrit were significantly decreased in TNF(ΔARE/+) mice, while erythropoiesis increased. These differences were not seen in IL-10(-/-) mice. Duodenal IL-6 and TNFα content increased significantly in TNF(ΔARE/+) mice, while ferritin-H decreased along with hepatic hepcidin expression, ferritin L, and non-haem iron. In IL-10(-/-) mice, these changes were less marked or missing for non-haem iron. Duodenal ferritin-L and ferroportin increased significantly, while HFE decreased. Our results corroborate the conflicting combination of low hepcidin with inflammation and without increased intestinal iron absorption. Speculating on underlying mechanism, decreased hepcidin may result from stimulated erythropoiesis. Unaltered intestinal iron-absorption may compromise between the stimulation by increased erythropoiesis and inhibition by local and systemic inflammation. The findings suggest intense interaction between counterproductive mechanisms and ask for further research.
authors: Chair of Animal Nutrition, TUM School of Life Sciences, Technische Universität München, Germany.
authors: M
authors: M
authors: Buffler
authors: Chair of Animal Nutrition, TUM School of Life Sciences, Technische Universität München, Germany. Electronic address: christiane.becker@wzw.tum.de.
authors: C
authors: C
authors: Becker
authors: Chair of Animal Nutrition, TUM School of Life Sciences, Technische Universität München, Germany.
authors: W
authors: W
authors: Windisch
authors: Research Center for Nutrition and Food Science (ZIEL), TUM School of Life Sciences, Technische Universität München, Germany.
authors: K
authors: K
authors: Schümann
mesh_terms: D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D004386:Duodenum / Q000378:metabolism; D005786:Gene Expression Regulation; D057898:Hemizygote; D064451:Hepcidins / Q000378:metabolism*; D006720:Homozygote; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D016753:Interleukin-10 / Q000172:deficiency* / Q000378:metabolism; D007408:Intestinal Absorption; D007422:Intestines / Q000473:pathology*; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D064451:Hepcidins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007501:Iron
keywords: Hepcidin; IL-10(−/−)-mice; Inflammation; Iron-absorption; TNF(ΔARE/+)-mice