title: Cardiolipin composition correlates with prostate cancer cell proliferation.
abstract: Prostate cancer (PC) is the second most diagnosed cancer in men. It has been recognized that diet can play a crucial role in PC genesis and progression. In this context, free fatty acids are considered as modulators of cell proliferation. Recently, a relationship between the composition of the mitochondrial phospholipid cardiolipin (CL) and cell proliferation has been discussed. The aim of this study was to analyse the interrelationship between CL composition and the proliferation of prostate cells by exposing PC-3 tumour cells to different fatty acids and by analysing the CL composition in prostate tissue from PC patients after prostatectomy. Among the applied fatty acids, palmitic acid was found to stimulate proliferation of PC-3 cells, whereas oleic acid (OA) had an inhibiting effect. The lipidomic analysis of CL revealed that fatty acids supplied to PC-3 cells were incorporated into CL molecules. Further, the CL content of palmitoleic acid (C16:1) exclusively correlated with the proliferation of PC-3 cells. The CL composition significantly differed between tumour and normal prostate tissue from PC patients. In five out of six patients, the CL content of palmitoleic acid was higher in tumour prostate tissue in comparison to normal prostate tissue. Our data illustrate that the composition of CL can be easily modified by the fatty acid environment of cells. OA was most effective in decreasing the amount of palmitoleic acid within the CL molecules and deceleration of PC-3 cell proliferation. In conclusion, a diet rich in OA might be beneficial in protecting from rapid proliferation of PC cells.
authors: Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.
authors: Anja
authors: A
authors: Sapandowski
authors: Department for Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Matthias
authors: M
authors: Stope
authors: Department for Pathology, University Medicine Greifswald, Greifswald, Germany.
authors: Katja
authors: K
authors: Evert
authors: Department for Pathology, University Medicine Greifswald, Greifswald, Germany.
authors: Matthias
authors: M
authors: Evert
authors: Department for Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Uwe
authors: U
authors: Zimmermann
authors: Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.
authors: Daniela
authors: D
authors: Peter
authors: Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.
authors: Ilona
authors: I
authors: Päge
authors: Department for Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Martin
authors: M
authors: Burchardt
authors: Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany. lorenz.schild@med.ovgu.de.
authors: Lorenz
authors: L
authors: Schild
mesh_terms: D000368:Aged; D002308:Cardiolipins / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D053719:Tandem Mass Spectrometry; D059016:Tumor Microenvironment
chemical_list: D002308:Cardiolipins; D005227:Fatty Acids
keywords: Cardiolipin; Free fatty acids; Lipidomic analysis; Prostate cancer; Tumour cell proliferation