title: Effects of Fe deficiency on the protein profile of Brassica napus phloem sap.
abstract: The aim of this work was to study the effect of Fe deficiency on the protein profile of phloem sap exudates from Brassica napus using 2DE (IEF-SDS-PAGE). The experiment was repeated thrice and two technical replicates per treatment were done. Phloem sap purity was assessed by measuring sugar concentrations. Two hundred sixty-three spots were consistently detected and 15.6% (41) of them showed significant changes in relative abundance (22 decreasing and 19 increasing) as a result of Fe deficiency. Among them, 85% (35 spots), were unambiguously identified. Functional categories containing the largest number of protein species showing changes as a consequence of Fe deficiency were signaling and regulation (32%), and stress and redox homeostasis (17%). The Phloem sap showed a higher oxidative stress and significant changes in the hormonal profile as a result of Fe deficiency. Results indicate that Fe deficiency elicits major changes in signaling pathways involving Ca and hormones, which are generally associated with flowering and developmental processes, causes an alteration in ROS homeostasis processes, and induces decreases in the abundances of proteins involved in sieve element repair, suggesting that Fe-deficient plants may have an impaired capacity to heal sieve elements upon injury.
authors: Plant Nutrition Department, CSIC, Aula Dei Experimental Station, Zaragoza, Spain.
authors: Elain
authors: E
authors: Gutierrez-Carbonell
authors: Plant Nutrition Department, CSIC, Aula Dei Experimental Station, Zaragoza, Spain.
authors: Giuseppe
authors: G
authors: Lattanzio
authors: Department of Plant Nutrition, CEBAS-CSIC, Campus Universitario de Espinardo, Murcia, Spain.
authors: Alfonso
authors: A
authors: Albacete
authors: Plant Nutrition Department, CSIC, Aula Dei Experimental Station, Zaragoza, Spain.
authors: Juan José
authors: JJ
authors: Rios
authors: Department of Molecular Plant Genetics, Biocenter Klein Flottbek, University Hamburg, Hamburg, Germany.
authors: Julia
authors: J
authors: Kehr
authors: Plant Nutrition Department, CSIC, Aula Dei Experimental Station, Zaragoza, Spain.
authors: Anunciación
authors: A
authors: Abadía
authors: USDA-ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
authors: Michael A
authors: MA
authors: Grusak
authors: Plant Nutrition Department, CSIC, Aula Dei Experimental Station, Zaragoza, Spain.
authors: Javier
authors: J
authors: Abadía
authors: Plant Nutrition Department, CSIC, Aula Dei Experimental Station, Zaragoza, Spain.|USDA-ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
authors: Ana Flor
authors: AF
authors: López-Millán
mesh_terms: D029688:Brassica napus / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007501:Iron / Q000378:metabolism*; D018384:Oxidative Stress; D052585:Phloem / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D007501:Iron
keywords: Bidimensional electrophoresis; Brassica napus; Hormones; Iron; Phloem Sap; Plant proteomics