title: Building cellular pathways and programs enabled by the genetic diversity of allo-genomes and meta-genomes.
abstract: Engineering pathways, programs and traits that require interactions of many, often unknown, genes requires advanced engineering strategies in the context of synthetic biology. Such strategies derive from three basic requirements: a suitably enlarged gene pool compared to the parent strain; a method of identification and incorporation of genetic-element interactions to generate the multigenic pathway or trait; and a process of selection of individuals from diverse strain populations that benefit the desirable pathway or trait. We review potential methods utilized in such advanced engineering strategies, emphasizing methods that explore the genomic diversity of allogeneic DNA (the allogenome) or the metagenome. We also propose a modular iterative approach for developing multigenic cellular traits.
authors: Molecular Biotechnology Laboratory, Department of Chemical & Biomolecular Engineering, The Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.
authors: Kyle A
authors: KA
authors: Zingaro
authors: Molecular Biotechnology Laboratory, Department of Chemical & Biomolecular Engineering, The Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA. Electronic address: epaps@udel.edu.
authors: Eleftherios Terry
authors: ET
authors: Papoutsakis
mesh_terms: D014644:Genetic Variation; D016678:Genome; D023281:Genomics; D006801:Humans; D010641:Phenotype; D012641:Selection, Genetic; D058615:Synthetic Biology / Q000379:methods