title: MicroRNA-107 contributes to post-stroke angiogenesis by targeting Dicer-1.
abstract: Previous studies have suggested that microRNA-107 (miR-107) regulates cell migration in tumor and promotes Hypoxia Inducible Factor 1α (HIF1α) regulated angiogenesis under hypoxia. We found that miR-107 was strongly expressed in ischemic boundary zone (IBZ) after permanent middle cerebral artery occlusion (pMCAO) in rats and inhibition of miR-107 could reduce capillary density in the IBZ after stroke. Such finding led us to hypothesize that miR-107 might regulate post-stroke angiogenesis and therefore serve as a therapeutic target. We also found that antagomir-107, a synthetic miR-107 inhibitor, decreased the number of capillaries in IBZ and increased overall infarct volume after pMCAO in rats. We demonstrated that miR-107 could directly down-regulate Dicer-1, a gene that encodes an enzyme essential for processing microRNA (miRNA) precursors. This resulted in translational desupression of VEGF (vascular endothelial growth factor) mRNA, thereby increasing expression of endothelial cell-derived VEGF (VEGF165/VEGF164), leading to angiogenesis after stroke. This process might be a protective mechanism for ischemia-induced cerebral injury and miR-107 might be used as a novel tool in stroke treatment.
authors: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Yanan
authors: Y
authors: Li
authors: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Ling
authors: L
authors: Mao
authors: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Yuan
authors: Y
authors: Gao
authors: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Suraj
authors: S
authors: Baral
authors: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Yifan
authors: Y
authors: Zhou
authors: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Bo
authors: B
authors: Hu
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002465:Cell Movement; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D020244:Infarction, Middle Cerebral Artery / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D055806:Microvessels / Q000473:pathology; D018919:Neovascularization, Physiologic; D017207:Rats, Sprague-Dawley; D043244:Ribonuclease III / Q000378:metabolism*; D020521:Stroke / Q000235:genetics* / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C579315:MIRN107 microRNA, rat; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; C583537:Dicer1 protein, rat; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases