title: Conformational change results in loss of enzymatic activity of jack bean urease on its interaction with silver nanoparticle.
abstract: Urease is an enzyme produced by microbes such as bacteria, yeast and fungi. Plants also produce this enzyme. Urease action splits urea into ammonia and carbamate. This action is having important implications in agro-chemical, medicinal and environment. Therefore there is always a constant search for new and novel compounds which could inhibit this enzyme. Here we have studied the interaction of jack bean urease (JBU) with silver nanoparticle to analyze the influence of the resultant protein corona formation on the catalytic property of JBU. Several techniques like UV-Vis, gel shift assay and CD spectroscopy have been used to characterize this interaction. Urease activity assay suggests that the protein corona formation inhibits the enzymatic action of JBU. The loss of enzymatic action could be either due to the nanoparticle blocking the active site of JBU or a conformational change in the protein. The CD spectra of JBU-AgNP complexes clearly revealed significant changes in the secondary structural composition of the JBU and this could be the reason for the loss of enzymatic activity of JBU. This study revealed an interesting observation, where the interaction of AgNP with JBU resulted destabilization of hexameric nature of JBU which is otherwise highly stable. The results of the present study could be useful in the development of nanoparticle based material for inhibiting the ureolytic activity of ureases in different fields.
authors: Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600 025, India.
authors: Shobana
authors: S
authors: Ponnuvel
authors: National Centre for Nanoscience and Nanotechnology, University of Madras, Guindy Campus, Chennai, India.
authors: Balakumar
authors: B
authors: Subramanian
authors: Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600 025, India. pkarthe@hotmail.com.
authors: Karthe
authors: K
authors: Ponnuraj
mesh_terms: D032449:Canavalia / Q000737:chemistry / Q000201:enzymology*; D020134:Catalytic Domain; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D012834:Silver / Q000737:chemistry / Q000378:metabolism*; D014510:Urease / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D010940:Plant Proteins; D012834:Silver; D014510:Urease
keywords: Circular dichroism (CD); Conformational change; Enzyme-nanoparticle corona; Jack bean urease (JBU); Silver nanoparticle (AgNP)