title: Genetic and biological characterization of two novel reassortant H5N6 swine influenza viruses in mice and chickens.
abstract: Novel H5N6 influenza A viruses have infected birds and human beings and caused four human clinical cases in China since 2014. The pig, as a mixing vessel, plays an important role for influenza virus reassortment and transmission. Towards this, routine surveillance for swine influenza in Guangdong province was conducted in 2014. In this study, we reported the biological characterization of two H5N6 influenza viruses isolated from healthy pigs in Guangdong province. Genetic analysis indicates that the two viruses are reassortants of 2.3.4.4 H5N1 and H6N6 avian influenza viruses with a high similarity to duck and human H5N6 influenza viruses isolated from Guangdong province. The data from chicken and mouse experiments show that the viruses are highly pathogenic in chickens and result in a systemic infection, and replicate in the mouse lung accompanying with a clinical inflammatory pathology. The results of the study demonstrate that the two H5N6 influenza viruses isolated from swine are the avian-originated viruses and have not adapted to swine population yet. However, they might keep evolving and pose a potential risk to public health and the continued surveillance of swine influenza should be strengthened.
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, China.
authors: Xuyong
authors: X
authors: Li
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Yuguang
authors: Y
authors: Fu
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Jiayun
authors: J
authors: Yang
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Jing
authors: J
authors: Guo
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Jijun
authors: J
authors: He
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Jianhong
authors: J
authors: Guo
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Shaoting
authors: S
authors: Weng
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Yane
authors: Y
authors: Jia
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Bin
authors: B
authors: Liu
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors: Xiaoyang
authors: X
authors: Li
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China. Electronic address: zhuqiyun@caas.cn.
authors: Qiyun
authors: Q
authors: Zhu
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, China. Electronic address: chenhualan@caas.cn.
authors: Hualan
authors: H
authors: Chen
mesh_terms: D000818:Animals; D002645:Chickens; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D009980:Influenza A virus / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D005585:Influenza in Birds / Q000821:virology*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D016865:Reassortant Viruses; D013552:Swine; D037521:Virulence Factors / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: H5N6; Influenza virus; Public health; Reassortment; Swine