title: PLD1 activation mediates Amb a 1-induced Th2-associated cytokine expression via the JNK/ATF-2 pathway in BEAS-2B cells.
abstract: The purpose of this study was to identify the role of phospholipase D1 (PLD1) in Amb a 1-induced IL-5 and IL-13 expression. When BEAS-2B cells were stimulated with Amb a 1, PLD activity increased, and knockdown of PLD1 decreased Amb a 1-induced IL-5 and IL-13 expression. Amb a 1 also activated the PLCγ/p70S6K/JNK pathway. Furthermore, Amb a 1-induced PLD activation was also attenuated by PLCγ inhibition, and knockdown of PLD1 decreased Amb a 1-induced activation of P70S6K and JNK. When ATF-2 activity was blocked with ATF-2 siRNA, Amb a 1-induced IL-5 and IL-13 expression was completely abolished, indicating that ATF-2 is a transcriptional factor required for the expression of IL-5 and IL-13 in response to Amb a 1. Taken together, we suggest that PLD1 acts as an important regulator in Amb a 1-induced expression of IL-5 and IL-13 via a PLCγ/p70S6K/JNK/ATF-2 pathway in BEAS-2B cells.
authors: Department of Pediatrics, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
authors: Joo-Hwa
authors: JH
authors: Kim
authors: Biomedical Research Institute and Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
authors: Hye-Jin
authors: HJ
authors: Choi
authors: Biomedical Research Institute and Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea.
authors: Cheong-Hae
authors: CH
authors: Oh
authors: Department of Pediatrics, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: jaewonoh@hanyang.ac.kr.
authors: Jae-Won
authors: JW
authors: Oh
authors: Biomedical Research Institute and Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: jshan@hanyang.ac.kr.
authors: Joong-Soo
authors: JS
authors: Han
mesh_terms: D051698:Activating Transcription Factor 2 / Q000235:genetics; D052179:Antigens, Plant / Q000276:immunology*; D002461:Cell Line, Transformed; D004789:Enzyme Activation; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000096:biosynthesis*; D048031:JNK Mitogen-Activated Protein Kinases / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000276:immunology; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors / Q000276:immunology; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000276:immunology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; C026967:Amb a I protein, Ambrosia artemisiifolia; D052179:Antigens, Plant; C508599:IL5 protein, human; D018793:Interleukin-13; D015848:Interleukin-5; D010940:Plant Proteins; D034741:RNA, Small Interfering; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D048031:JNK Mitogen-Activated Protein Kinases; C105606:phospholipase D2; D051966:Phospholipase C gamma; D010739:Phospholipase D; C106801:phospholipase D1
keywords: Ambrosia artemisiifolia; IL-13; IL-5; JNK; PLD1; p70S6K