title: A biochemical screening approach to putatively differentiate mammalian pathogenic Oomycota species in the clinical laboratory.
abstract: The report of four novel mammalian pathogenic species of the genus Lagenidium prompted us to study the use of biochemical assays to differentiate the Oomycota mammalian pathogens Pythium insidiosum and Lagenidium spp. We investigated the reaction of 23 Lagenidium and eight Pythium species in various biochemical assays. Because the morphological features of the Oomycota species are similar to those of species in the Entomophthoramycota and Mucormycota, five fungal species with coenocytic hyphae were also included. We found that mammalian and plant isolates of Pythium spp. all hydrolysed sucrose, but Lagenidium species and the fungal strains did not. In addition, both Pythium spp. and Lagenidium spp. were found to be maltose-positive, whereas fungal strains did not hydrolyse this sugar. The fungal species and thermo-sensitive Lagenidium giganteum and Lagenidium humanum were urease-negative, but the mammalian Lagenidium spp. and Pythium spp. hydrolysed urea within 24  h. These findings suggest these assays can be used for the presumptive differentiation of mammalian Oomycota species in the laboratory.
authors: Biomedical Laboratory Diagnostics, Michigan State University, East Lansing, MI 48824, USA.|Superior Institute of Medicine, Belo Horizonte, Brazil.|Faculty of Pharmacy, Universidade Federal of Minas Gerais, Belo Horizonte, Brazil.
authors: Raquel
authors: R
authors: Vilela
authors: Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
authors: Poorna
authors: P
authors: Viswanathan
authors: Biomedical Laboratory Diagnostics, Michigan State University, East Lansing, MI 48824, USA.|Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
authors: Leonel A
authors: LA
authors: Mendoza
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D006801:Humans; D044726:Lagenidium / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008322:Mammals; D008403:Mass Screening / Q000379:methods*; D008828:Microbiological Techniques / Q000379:methods*; D010944:Plants; D011775:Pythium / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D014510:Urease / Q000032:analysis
chemical_list: D014510:Urease