title: Regulation of MDA5-MAVS Antiviral Signaling Axis by TRIM25 through TRAF6-Mediated NF-κB Activation.
abstract: Tripartite motif protein 25 (TRIM25), mediates K63-linked polyubiquitination of Retinoic acid inducible gene I (RIG-I) that is crucial for downstream antiviral interferon signaling. Here, we demonstrate that TRIM25 is required for melanoma differentiation-associated gene 5 (MDA5) and MAVS mediated activation of NF-κB and interferon production. TRIM25 is required for the full activation of NF-κB at the downstream of MAVS, while it is not involved in IRF3 nuclear translocation. Mechanical studies showed that TRIM25 is involved in TRAF6-mediated NF-κB activation. These collectively indicate that TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-κB.
authors: Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea.
authors: Na-Rae
authors: NR
authors: Lee
authors: Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea.
authors: Hye-In
authors: HI
authors: Kim
authors: Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea.
authors: Myung-Soo
authors: MS
authors: Choi
authors: Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea.
authors: Chae-Min
authors: CM
authors: Yi
authors: Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea.
authors: Kyung-Soo
authors: KS
authors: Inn
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D000072640:Interferon-Induced Helicase, IFIH1; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000502:physiology*; D014777:Virus Diseases / Q000276:immunology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; C505371:MAVS protein, human; D016328:NF-kappa B; D048029:TNF Receptor-Associated Factor 6; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; C084523:TRIM25 protein, human; D044767:Ubiquitin-Protein Ligases; C452815:IFIH1 protein, human; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1
keywords: MAVS; MDA5; NF-κB; TRAF6; TRIM25