title: Mycobacterium massiliense Type II genotype leads to higher level of colony forming units and TNF-α secretion from human monocytes than Type I genotype.
abstract: Recently, we introduced a novel Mycobacterium massiliense Type II genotype from Korean patients, in which all isolates showed only a rough (R) colony morphotype. In this study, we sought to compare clinical factors and virulence potentials of two genotypes of M. massiliense, Type I and Type II. Patients infected with Type II tend to be younger at infection than those infected with Type I (56.7 vs 62.3, p = 0.051). Type II was more significantly related to R colony type than Type I (34.1% vs 94.1%, p < 0.001). The Type II strain showed significantly more colony forming units (CFUs) and higher levels of TNF-α secretion in infection of human monocytes than the Type I strain. The challenge of extracted glycopeptidolipid (GPL) into human monocytes indicated that the loss of GPL from the cell wall of the Type II genotype led to a higher level of TNF-α secretion in a toll-like receptor 2(TLR2)-dependent manner. Taken together, our data suggest that the M. massiliense Type II genotype shows higher virulence than Type I, which may be due to the induction of TNF-α via the loss of GPL from the Type II cell wall.
authors: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Korea.
authors: Byoung-Jun
authors: BJ
authors: Kim
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors: Tae Sun
authors: TS
authors: Shim
authors: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Korea.
authors: Su-Yeon
authors: SY
authors: Yi
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors: Ho-Cheol
authors: HC
authors: Kim
authors: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Korea.
authors: Bo-Ram
authors: BR
authors: Kim
authors: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Korea.
authors: So-Young
authors: SY
authors: Lee
authors: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Korea.
authors: Yoon-Hoh
authors: YH
authors: Kook
authors: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Korea.
authors: Bum-Joon
authors: BJ
authors: Kim
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D005260:Female; D006017:Glycolipids / Q000235:genetics / Q000276:immunology; D006801:Humans; D006973:Hypertension / Q000150:complications; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D009164:Mycobacterium Infections / Q000382:microbiology*; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D006017:Glycolipids; C495342:TLR2 protein, human; C439524:TNF protein, human; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha
keywords: Mycobacterium massiliense; TNF-α; Type II genotype; colony forming units; glycopeptidolipid