title: Predicting cortical bone adaptation to axial loading in the mouse tibia.
abstract: The development of predictive mathematical models can contribute to a deeper understanding of the specific stages of bone mechanobiology and the process by which bone adapts to mechanical forces. The objective of this work was to predict, with spatial accuracy, cortical bone adaptation to mechanical load, in order to better understand the mechanical cues that might be driving adaptation. The axial tibial loading model was used to trigger cortical bone adaptation in C57BL/6 mice and provide relevant biological and biomechanical information. A method for mapping cortical thickness in the mouse tibia diaphysis was developed, allowing for a thorough spatial description of where bone adaptation occurs. Poroelastic finite-element (FE) models were used to determine the structural response of the tibia upon axial loading and interstitial fluid velocity as the mechanical stimulus. FE models were coupled with mechanobiological governing equations, which accounted for non-static loads and assumed that bone responds instantly to local mechanical cues in an on-off manner. The presented formulation was able to simulate the areas of adaptation and accurately reproduce the distributions of cortical thickening observed in the experimental data with a statistically significant positive correlation (Kendall's τ rank coefficient τ = 0.51, p < 0.001). This work demonstrates that computational models can spatially predict cortical bone mechanoadaptation to a time variant stimulus. Such models could be used in the design of more efficient loading protocols and drug therapies that target the relevant physiological mechanisms.
authors: Department of Bioengineering, Imperial College London, London, UK andrepere@gmail.com.
authors: A F
authors: AF
authors: Pereira
authors: Comparative Biomedical Sciences, Royal Veterinary College, London, UK.
authors: B
authors: B
authors: Javaheri
authors: Comparative Biomedical Sciences, Royal Veterinary College, London, UK.
authors: A A
authors: AA
authors: Pitsillides
authors: Department of Mechanical and Industrial Engineering and Department of Bioengineering, Northeastern University, Boston, MA, USA.
authors: S J
authors: SJ
authors: Shefelbine
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D020342:Finite Element Analysis; D051379:Mice; D008954:Models, Biological; D013977:Tibia / Q000378:metabolism*; D016474:Weight-Bearing / Q000502:physiology
keywords: bone mechanobiology; cortical thickness; fluid flow; functional adaptation; mouse tibia