title: The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability.
abstract: Jasmonate (JA) signaling in plants is mediated by the JASMONATE ZIM-DOMAIN (JAZ) proteins that repress the activity of several transcription factors regulating JA-inducible gene expression. The hormone JA-isoleucine triggers the interaction of JAZ repressor proteins with the F-box protein CORONATINE INSENSITIVE1 (COI1), part of an S-phase kinase-associated protein1/Cullin1/F-box protein COI1 (SCF(COI1)) E3 ubiquitin ligase complex, and their degradation by the 26S proteasome. In Arabidopsis (Arabidopsis thaliana), the JAZ family consists of 13 members. The level of redundancy or specificity among these members is currently not well understood. Here, we characterized JAZ12, encoded by a highly expressed JAZ gene. JAZ12 interacted with the transcription factors MYC2, MYC3, and MYC4 in vivo and repressed MYC2 activity. Using tandem affinity purification, we found JAZ12 to interact with SCF(COI1) components, matching with observed in vivo ubiquitination and with rapid degradation after treatment with JA. In contrast to the other JAZ proteins, JAZ12 also interacted directly with the E3 RING ligase KEEP ON GOING (KEG), a known repressor of the ABSCISIC ACID INSENSITIVE5 transcription factor in abscisic acid signaling. To study the functional role of this interaction, we circumvented the lethality of keg loss-of-function mutants by silencing KEG using an artificial microRNA approach. Abscisic acid treatment promoted JAZ12 degradation, and KEG knockdown led to a decrease in JAZ12 protein levels. Correspondingly, KEG overexpression was capable of partially inhibiting COI1-mediated JAZ12 degradation. Our results provide additional evidence for KEG as an important factor in plant hormone signaling and a positive regulator of JAZ12 stability.
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Laurens
authors: http://orcid.org/0000-0002-0221-9052
authors: L
authors: Pauwels
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Andrés
authors: A
authors: Ritter
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Jonas
authors: J
authors: Goossens
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Astrid Nagels
authors: AN
authors: Durand
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Hongxia
authors: http://orcid.org/0000-0002-9232-5122
authors: H
authors: Liu
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Yangnan
authors: http://orcid.org/0000-0001-5135-0180
authors: Y
authors: Gu
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Jan
authors: http://orcid.org/0000-0002-1003-1228
authors: J
authors: Geerinck
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Marta
authors: http://orcid.org/0000-0001-8688-5513
authors: M
authors: Boter
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Robin
authors: http://orcid.org/0000-0002-4593-9658
authors: R
authors: Vanden Bossche
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Rebecca
authors: http://orcid.org/0000-0002-4068-1811
authors: R
authors: De Clercq
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Jelle
authors: http://orcid.org/0000-0002-4932-8192
authors: J
authors: Van Leene
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Kris
authors: http://orcid.org/0000-0002-4237-0283
authors: K
authors: Gevaert
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Geert
authors: http://orcid.org/0000-0001-6558-5669
authors: G
authors: De Jaeger
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Roberto
authors: R
authors: Solano
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Sophia
authors: S
authors: Stone
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Roger W
authors: RW
authors: Innes
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
authors: Judy
authors: http://orcid.org/0000-0002-0622-078X
authors: J
authors: Callis
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.) algoo@psb.vib-ugent.be.
authors: Alain
authors: http://orcid.org/0000-0002-1599-551X
authors: A
authors: Goossens
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D009154:Mutation; D030821:Plants, Genetically Modified; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C112279:COI1 protein, Arabidopsis; C000609132:JAZ12 protein, Arabidopsis; C473611:MYC2 protein, Arabidopsis; D012097:Repressor Proteins; D000040:Abscisic Acid; C518495:KEG protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases