title: [Qualitative and quantitative analysis of major constituents of Paeoniae Radix Alba and Paeoniae Radix Rubra by HPLC-DAD-Q-TOF-MS/MS].
abstract: In order to explore the differences of chemical constituents of Paeoniae Radix Alba and Paeoniae Radix Rubra, a qualitative analytical method of liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS/MS) was developed for identification of multi-constituents and an HPLC-DAD analytical method was developed for simultaneously determining 14 major compounds (gallic acid, protocatechuic acid, paeoniflorin sulfonate, protocatechuic aldehyde, methyl gallate, oxypaeoniflorin, catechin, albiflorin, and paeoniflorin, ethyl gallate, benzoic acid, pentagaloylglucose, benzoyl-paeoniflorin, and paeonol) in Paeoniae Radix Alba and Paeoniae Radix Rubra. Q-TOF/MS qualitative analysis was performed under negative ion mode and inferred 38 components of Paeoniae Radix Alba and 30 components of Paeoniae Radix Rubra. HPLC-DAD quantitative method result showed the contents of 8 ingredients were different between Paeoniae Radix Alba and Paeoniae Radix Rubra. The results indicated that the new approach was applicable in qualitative and quantitative quality control of Paeoniae Radix Alba and Paeoniae Radix Rubra.
authors: Jie
authors: J
authors: Liu
authors: Lin
authors: L
authors: Chen
authors: Cai-rong
authors: CR
authors: Fan
authors: Huang
authors: H
authors: Li
authors: Ming-qing
authors: MQ
authors: Huang
authors: Qing
authors: Q
authors: Xiang
authors: Wen
authors: W
authors: Xu
authors: Wei
authors: W
authors: Xu
authors: Ke-dan
authors: KD
authors: Chu
authors: Yu
authors: Y
authors: Lin
mesh_terms: D001952:Bridged-Ring Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry; D029596:Paeonia / Q000737:chemistry* / Q000145:classification; D053719:Tandem Mass Spectrometry / Q000379:methods*; D006027:Glycosides
chemical_list: D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin