title: The COUP-TFII/Neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain.
abstract: The preoptic area (POa) of the rostral diencephalon supplies the neocortex and the amygdala with GABAergic neurons in the developing mouse brain. However, the molecular mechanisms that determine the pathway and destinations of POa-derived neurons have not yet been identified. Here we show that Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII)-induced expression of Neuropilin-2 (Nrp2) and its down-regulation control the destination of POa-derived GABAergic neurons. Initially, a majority of the POa-derived migrating neurons express COUP-TFII and form a caudal migratory stream toward the caudal subpallium. When a subpopulation of cells steers toward the neocortex, they exhibit decreased expression of COUP-TFII and Nrp2. The present findings show that suppression of COUP-TFII/Nrp2 changed the destination of the cells into the neocortex, whereas overexpression of COUP-TFII/Nrp2 caused cells to end up in the medial part of the amygdala. Taken together, these results reveal that COUP-TFII/Nrp2 is a molecular switch determining the pathway and destination of migrating GABAergic neurons born in the POa.
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan; Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden;
authors: Shigeaki
authors: S
authors: Kanatani
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Takao
authors: T
authors: Honda
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Michihiko
authors: M
authors: Aramaki
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Kanehiro
authors: K
authors: Hayashi
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Ken-ichiro
authors: K
authors: Kubo
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Mami
authors: M
authors: Ishida
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Daisuke H
authors: DH
authors: Tanaka
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan;
authors: Takeshi
authors: http://orcid.org/0000-0002-8650-4474
authors: T
authors: Kawauchi
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Katsutoshi
authors: K
authors: Sekine
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan;
authors: Sayaka
authors: S
authors: Kusuzawa
authors: Division of Brain Function, National Institute of Genetics, Graduate University for Advanced Studies (Sokendai), Mishima 411-8540, Japan;
authors: Takahiko
authors: T
authors: Kawasaki
authors: Division of Brain Function, National Institute of Genetics, Graduate University for Advanced Studies (Sokendai), Mishima 411-8540, Japan;
authors: Tatsumi
authors: T
authors: Hirata
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai 713-8, Japan.
authors: Hidenori
authors: H
authors: Tabata
authors: Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden;
authors: Per
authors: P
authors: Uhlén
authors: Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan; kazunori@keio.jp.
authors: Kazunori
authors: K
authors: Nakajima
mesh_terms: D000679:Amygdala / Q000196:embryology / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D051837:COUP Transcription Factor II / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism*; D059330:GABAergic Neurons / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D019579:Neocortex / Q000196:embryology / Q000378:metabolism; D039943:Neuropilin-2 / Q000235:genetics / Q000378:metabolism*; D011301:Preoptic Area / Q000196:embryology / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046509:Tissue Culture Techniques
chemical_list: D051837:COUP Transcription Factor II; D039943:Neuropilin-2
keywords: COUP-TFII/Neuropilin-2; amygdala; caudal migratory stream; cortex; preoptic area