title: Tsukushi expression is dependent on Notch signaling and oscillated in the presomitic mesoderm during chick somitogenesis.
abstract: During somitogenesis, segmentation of the body axis occurs by epithelial somites budding off from the rostral end of the unsegmented presomitic mesoderm (PSM), and its molecular regulation is achieved by a molecular oscillator and signaling molecules. Tsukushi (TSK) is a unique secreted protein and involved in diverse biological cascades in vertebrate embryos by modulating several signaling pathways at the extracellular region. However, the involvement of TSK in somitogenesis remains unknown. In this study, we investigated the detailed expression patterns of TSK at different developmental stages of a chick embryo. Chick-TSK (C-TSK) is expressed in the PSM and shows an oscillation pattern with three phases. The oscillation pattern of C-TSK in the PSM is similar to that of c-Notch1 and c-hairy1, but not to c-Delta1. Our in vitro data showed that Notch signaling is necessary for the normal expression of C-TSK and that expression of C-TSK is an intrinsic property of the anterior PSM. These data suggest that TSK plays a role in chick somitogenesis.
authors: Division of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan; Program for Leading Graduate Schools HIGO (Health Life Science: Interdisciplinary and Glocal Oriented), Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors: Uzzal Kumar
authors: UK
authors: Acharjee
authors: Division of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors: Ryu
authors: R
authors: Gejima
authors: Division of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors: M
authors: M
authors: Felemban Athary Abdulhaleem
authors: Division of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors: M Asrafuzzaman
authors: MA
authors: Riyadh
authors: Division of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors: Hideaki
authors: H
authors: Tanaka
authors: Division of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan; Japan Agency for Medical Research and Development (AMED), Tokyo 100-0004, Japan. Electronic address: ohta9203@gpo.kumamoto-u.ac.jp.
authors: Kunimasa
authors: K
authors: Ohta
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D002642:Chick Embryo; D047108:Embryonic Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D019170:Somites / Q000502:physiology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011509:Proteoglycans; D051880:Receptors, Notch; C578542:TSKU protein, human
keywords: Chick; Notch signaling; Oscillation; Somitogenesis; Tsukushi