title: High-throughput droplet analysis and multiplex DNA detection in the microfluidic platform equipped with a robust sample-introduction technique.
abstract: In this work, a simple, flexible and low-cost sample-introduction technique was developed and integrated with droplet platform. The sample-introduction strategy was realized based on connecting the components of positive pressure input device, sample container and microfluidic chip through the tygon tubing with homemade polydimethylsiloxane (PDMS) adaptor, so the sample was delivered into the microchip from the sample container under the driving of positive pressure. This sample-introduction technique is so robust and compatible that could be integrated with T-junction, flow-focus or valve-assisted droplet microchips. By choosing the PDMS adaptor with proper dimension, the microchip could be flexibly equipped with various types of familiar sample containers, makes the sampling more straightforward without trivial sample transfer or loading. And the convenient sample changing was easily achieved by positioning the adaptor from one sample container to another. Benefiting from the proposed technique, the time-dependent concentration gradient was generated and applied for quantum dot (QD)-based fluorescence barcoding within droplet chip. High-throughput droplet screening was preliminarily demonstrated through the investigation of the quenching efficiency of ruthenium complex to the fluorescence of QD. More importantly, multiplex DNA assay was successfully carried out in the integrated system, which shows the practicability and potentials in high-throughput biosensing.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors: Jinyang
authors: J
authors: Chen
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors: Xinghu
authors: X
authors: Ji
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Suzhou Institute of Wuhan University, Suzhou 215123, PR China. Electronic address: zhkhe@whu.edu.cn.
authors: Zhike
authors: Z
authors: He
mesh_terms: D004247:DNA / Q000032:analysis*; D058893:DNA Barcoding, Taxonomic / Q000191:economics / Q000295:instrumentation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000191:economics / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D004247:DNA
keywords: Droplet analysis; Fluorescence; Multiplex DNA assay; Quantum dot; Sample-introduction