title: Candidate Resistant Genes of Sand Pear (Pyrus pyrifolia Nakai) to Alternaria alternata Revealed by Transcriptome Sequencing.
abstract: Pear black spot (PBS) disease, which is caused by Alternaria alternata (Aa), is one of the most serious diseases affecting sand pear (Pyrus pyrifolia Nakai) cultivation worldwide. To investigate the defense mechanisms of sand pear in response to Aa, the transcriptome of a sand pear germplasm with differential resistance to Aa was analyzed using Illumina paired-end sequencing. Four libraries derived from PBS-resistant and PBS-susceptible sand pear leaves were characterized through inoculation or mock-inoculation. In total, 20.5 Gbp of sequence data and 101,632,565 reads were generated, representing 44717 genes. Approximately 66% of the genes or sequenced reads could be aligned to the pear reference genome. A large number (5213) of differentially expressed genes related to PBS resistance were obtained; 34 microsatellites were detected in these genes, and 28 genes were found to be closely related to PBS resistance. Using a transcriptome analysis in response to PBS inoculation and comparison analysis to the PHI database, 4 genes (Pbr039001, Pbr001627, Pbr025080 and Pbr023112) were considered to be promising candidates for sand pear resistance to PBS. This study provides insight into changes in the transcriptome of sand pear in response to PBS infection, and the findings have improved our understanding of the resistance mechanism of sand pear to PBS and will facilitate future gene discovery and functional genome studies of sand pear.
authors: School of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, P. R. China; Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Xiaoping
authors: X
authors: Yang
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Hongju
authors: H
authors: Hu
authors: School of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, P. R. China; Hubei Laboratory of Crop Diseases, Insect Pests and Weeds Control, Wuhan, Hubei, 430064, P. R. China.
authors: Dazhao
authors: D
authors: Yu
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Zhonghai
authors: Z
authors: Sun
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Xiujuan
authors: X
authors: He
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Jingguo
authors: J
authors: Zhang
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Qiliang
authors: Q
authors: Chen
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Rui
authors: R
authors: Tian
authors: Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei, 430064, P. R. China.
authors: Jing
authors: J
authors: Fan
mesh_terms: D000528:Alternaria / Q000472:pathogenicity*; D060487:Alternariosis / Q000235:genetics / Q000382:microbiology; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D018745:Genome, Plant / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D031989:Pyrus / Q000235:genetics* / Q000382:microbiology*; D059467:Transcriptome / Q000235:genetics*