title: Strain-specific induction of endometrial periglandular fibrosis in mice exposed during adulthood to the endocrine disrupting chemical bisphenol A.
abstract: The aim of this study was to compare effects of bisphenol A (BPA) on collagen accumulation in uteri of two mouse strains. Adult C57Bl/6N and CD-1 mice were exposed to dietary BPA (0.004-40mg/kg/day) or 17α-ethinyl estradiol (0.00002-0.001mg/kg/day) as effect control. An equine endometrosis-like phenotype with increased gland nesting and periglandular collagen accumulation was characteristic of unexposed C57Bl/6N, but not CD-1, endometrium. BPA non-monotonically increased gland nest density and periglandular collagen accumulation in both strains. Increased collagen I and III expression, decreased matrix metalloproteinase 2 (MMP2) and MMP14 expression, and increased immune response were associated with the endometrosis phenotype in the C57Bl/6N strain and the 30ppm BPA CD-1 group. The association between the pro-collagen shift in increased collagen expression and decreased MMP2 expression and activity implies that strain differences and BPA exposure alter regulation of endometrial remodeling and contribute to increased fibrosis, a component of several human uterine diseases.
authors: Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0575, USA.
authors: Jessica A
authors: JA
authors: Kendziorski
authors: Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0575, USA. Electronic address: scott.belcher@uc.edu.
authors: Scott M
authors: SM
authors: Belcher
mesh_terms: D000367:Age Factors; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D004715:Endometriosis / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005355:Fibrosis; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000633:toxicity*; D010641:Phenotype; D013045:Species Specificity; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D024042:Collagen Type I; D024061:Collagen Type III; D052244:Endocrine Disruptors; D053511:Matrix Metalloproteinase 14; D020778:Matrix Metalloproteinase 2; D010636:Phenols; C507096:Mmp14 protein, mouse; C522362:Mmp2 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: BPA; Collagen; Dose response; Endocrine disruptor; Endometrial periglandular fibrosis; Equine endometrosis; Matrix metalloproteinase; Mouse; Non-monotonic; Uterus