title: Differential induction of meristematic stem cells of Catharanthus roseus and their characterization.
abstract: Plant cell culture technology has been introduced for the mass production of the many useful components. A variety of plant-derived compounds is being used in various fields, such as pharmaceuticals, foods, and cosmetics. Plant cell cultures are believed to be derived from the dedifferentiation process. In the present study, an undifferentiated cambial meristematic cell (CMCs) of Catharanthus is isolated using histological and genetic methods, and compared with dedifferentiation-derived callus (DDCs) cultures. Furthermore, differential culture conditions for both DDCs- and CMCs-derived cell lines were established. A suitable media for the increased accumulation of terpenoid indole alkaloids (TIAs) was also standardized. Compared with DDCs, CMCs showed marked accumulation of TIAs in cell lines grown on media with 1.5 mg·mL(-1) of NAA and 0.5 mg·mL(-1) of kinetin. CMCs-derived cultures of Catharanthus, as a source of key anticancer drugs (viblastine and vincristine), would overcome the obstacles usually associated with the production of natural metabolites through the use of DDCs. Cell culture systems that are derived from CMCs may also provide a cost-effective and eco-friendly basis for the sustainable production of a number of important plant natural products.
authors: Department of Bioresource and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea.
authors: So Hyun
authors: SH
authors: Moon
authors: Department of Bioresource and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea.
authors: Jelli
authors: J
authors: Venkatesh
authors: Department of Bioresource and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea.
authors: Jae-Woong
authors: JW
authors: Yu
authors: Department of Bioresource and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea. Electronic address: sewpark@konkuk.ac.kr.
authors: Se Won
authors: SW
authors: Park
mesh_terms: D058506:Cambium / Q000166:cytology; D030008:Catharanthus / Q000166:cytology*; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation; D002460:Cell Line; D003470:Culture Media; D018519:Meristem / Q000166:cytology*; D059828:Plant Cells / Q000378:metabolism; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D013234:Stem Cells / Q000166:cytology*
chemical_list: D003470:Culture Media; D046948:Secologanin Tryptamine Alkaloids
keywords: Callus; Cambial meristematic cells; Catharanthus roseus; Dedifferentiation; Terpenoid indole alkaloids biosynthesis