title: An ethanolamine kinase Eki1 affects radial growth and cell wall integrity in Trichoderma reesei.
abstract: Ethanolamine kinase (ATP:ethanolamine O-phosphotransferase, EC 2.7.1.82) catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway. The functions of eki genes that encode ethanolamine kinase have been intensively studied in mammalian cells, fruit flies and yeast. However, the role of the eki gene has not yet been characterized in filamentous fungi. In this study, Treki1, an ortholog of Saccharomyces cerevisiae EKI1, was identified and functionally characterized using a target gene deletion strategy in Trichoderma reesei. A Treki deletion mutant was less sensitive to cell wall stressors calcofluor white and Congo red and released fewer protoplasts during cell wall digestion than the parent strain QM9414. Further transcription analysis showed that the expression levels of five genes that encode chitin synthases were drastically increased in the ΔTreki1 mutant. The chitin content was also increased in the null mutant of Treki1 comparing to the parent strain. In addition, the ΔTreki1 mutant exhibited defects in radial growth, conidiation and the accumulation of ethanolamine. The results indicate that Treki1 plays a key role in growth and development and in the maintenance of cell wall integrity in T. reesei.
authors: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, Tianjin 300457, PR China Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors: Ronglin
authors: R
authors: He
authors: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China.
authors: Wei
authors: W
authors: Guo
authors: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China zhang_dy@tib.cas.cn.
authors: Dongyuan
authors: D
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001557:Benzenesulfonates / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism; D002687:Chitin Synthase / Q000235:genetics / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D003224:Congo Red / Q000494:pharmacology; D019856:Ethanolamine / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013172:Spores, Fungal / Q000254:growth & development; D014242:Trichoderma / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development* / Q000648:ultrastructure
chemical_list: D001557:Benzenesulfonates; D004396:Coloring Agents; D005456:Fluorescent Dyes; D029701:Saccharomyces cerevisiae Proteins; D002686:Chitin; D003224:Congo Red; D019856:Ethanolamine; C007061:C.I. Fluorescent Brightening Agent 28; D002687:Chitin Synthase; D017853:Phosphotransferases (Alcohol Group Acceptor); C020125:ethanolamine kinase
keywords: cell wall integrity maintenance; conidiation; ethanolamine accumulation; ethanolamine kinase