title: Lactococcus lactis V7 inhibits the cell invasion of bovine mammary epithelial cells by Escherichia coli and Staphylococcus aureus.
abstract: Bovine mastitis, an inflammatory disease of the mammary gland often associated to bacterial infection, is the first cause of antibiotic use in dairy cattle. Because of the risk of antibioresistance emergence, alternative non-antibiotic strategies are needed to prevent or to cure bovine mastitis and reduce the antibiotic use in veterinary medicine. In this work, we investigated Lactococcus lactis V7, a strain isolated from the mammary gland, as a probiotic option against bovine mastitis. Using bovine mammary epithelial cell (bMEC) culture, and two representative strains for Escherichia coli and for Staphylococcus aureus, two major mastitis pathogens, we investigated L. lactis V7 ability to inhibit cell invasion (i.e. adhesion and internalization) of these pathogens into bMEC. L. lactis V7 ability to modulate the production of CXCL8, a key chemokine IL-8 responsible for neutrophil influx, in bMEC upon challenge with E. coli was investigated by an ELISA dosage of CXCL8 in bMEC culture supernatants. We showed that L. lactis V7 inhibited the internalisation of both E. coli and S. aureus strains into bMEC, whereas it inhibited the adhesion of only one out of the two S. aureus strains and of none of the E. coli strains tested. Investigation of the bMEC immune response showed that L. lactis V7 alone induced a slight increase in CXCL8 production in bMEC and that it increased the inflammatory response in bMEC challenged with the E. coli strains. Altogether these features of L. lactis V7 make it a potential promising candidate for a probiotic prevention strategy against bovine mastitis.
authors: 1 INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042 Rennes, France.|2 Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042 Rennes, France.|3 Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270901, Brazil.
authors: B Seridan
authors: BS
authors: Assis
authors: 4 INRA, UMR 1282 Infectiologie et Santé Publique, 37380 Nouzilly, France.|5 Université François Rabelais de Tours, UMR1282, Infectiologie et Santé Publique, 37000 Tours, France.
authors: P
authors: P
authors: Germon
authors: 6 Departamento de Engenharia de Alimentos - Campus Sete Lagoas, Universidade Federal de São João del-Rey, Sete Lagoas, MG 36307-352, Brazil.
authors: A M
authors: AM
authors: Silva
authors: 1 INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042 Rennes, France.|2 Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042 Rennes, France.
authors: S
authors: S
authors: Even
authors: 3 Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270901, Brazil.
authors: J R
authors: JR
authors: Nicoli
authors: 1 INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042 Rennes, France.|2 Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042 Rennes, France.
authors: Y
authors: Y
authors: Le Loir
mesh_terms: D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion; D002417:Cattle; D002460:Cell Line; D004705:Endocytosis; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D016209:Interleukin-8 / Q000032:analysis; D013294:Lactococcus lactis / Q000502:physiology*; D008414:Mastitis, Bovine / Q000517:prevention & control / Q000628:therapy; D019936:Probiotics / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D016209:Interleukin-8
keywords: Escherichia coli; Lactococcus lactis; Staphylococcus aureus; cell invasion; inflammatory response; mammary probiotics; mastitis