title: Next-generation sequencing: hype and hope for development of personalized radiation therapy?
abstract: The introduction of next-generation sequencing (NGS) in the field of cancer research has boosted worldwide efforts of genome-wide personalized oncology aiming at identifying predictive biomarkers and novel actionable targets. Despite considerable progress in understanding the molecular biology of distinct cancer entities by the use of this revolutionary technology and despite contemporaneous innovations in drug development, translation of NGS findings into improved concepts for cancer treatment remains a challenge. The aim of this article is to describe shortly the NGS platforms for DNA sequencing and in more detail key achievements and unresolved hurdles. A special focus will be given on potential clinical applications of this innovative technique in the field of radiation oncology.
authors: Department of Radiooncology and Radiotherapy, Charité University Hospital Berlin, Translational Radiation Oncology Research Laboratory, Charitéplatz 1, 10117, Berlin, Germany. ingeborg.tinhofer@charite.de.|German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK) partner site, Heidelberg, Germany. ingeborg.tinhofer@charite.de.
authors: Ingeborg
authors: I
authors: Tinhofer
authors: Department of Radiooncology and Radiotherapy, Charité University Hospital Berlin, Translational Radiation Oncology Research Laboratory, Charitéplatz 1, 10117, Berlin, Germany.|German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK) partner site, Heidelberg, Germany.
authors: Franziska
authors: F
authors: Niehr
authors: Department of Radiooncology and Radiotherapy, Charité University Hospital Berlin, Translational Radiation Oncology Research Laboratory, Charitéplatz 1, 10117, Berlin, Germany.|German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK) partner site, Heidelberg, Germany.
authors: Robert
authors: R
authors: Konschak
authors: German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK) partner site, Heidelberg, Germany.
authors: Sandra
authors: S
authors: Liebs
authors: Group for Computational Modelling in Medicine, Institute for Theoretical Biology, Humboldt Universität, Berlin, Germany.
authors: Matthias
authors: M
authors: Munz
authors: Institute of Pathology, University Hospital and National Center for Tumor Diseases, Heidelberg, Germany.
authors: Albrecht
authors: A
authors: Stenzinger
authors: Institute of Pathology, University Hospital and National Center for Tumor Diseases, Heidelberg, Germany.|Institute of Pathology, University Hospital and National Center for Tumor Diseases, Heidelberg, Germany.
authors: Wilko
authors: W
authors: Weichert
authors: Charité Comprehensive Cancer Center, Charité University Hospital, Berlin, Germany.
authors: Ulrich
authors: U
authors: Keilholz
authors: Department of Radiooncology and Radiotherapy, Charité University Hospital Berlin, Translational Radiation Oncology Research Laboratory, Charitéplatz 1, 10117, Berlin, Germany.|German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK) partner site, Heidelberg, Germany.
authors: Volker
authors: V
authors: Budach
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000639:trends; D006801:Humans; D009369:Neoplasms / Q000532:radiotherapy*; D057285:Precision Medicine / Q000379:methods* / Q000639:trends; D011878:Radiotherapy / Q000379:methods* / Q000639:trends