title: Lipidic nanovesicles stabilize suspensions of metal oxide nanoparticles.
abstract: We have studied the effect of adding lipid nanovesicles (liposomes) on the aggregation of commercial titanium oxide (TiO2), zinc oxide (ZnO), or cerium oxide (CeO2) nanoparticles (NPs) suspensions in Hepes buffer. Liposomes were prepared with pure phospholipids or mixtures of phospholipids and/or cholesterol. Changes in turbidity were recorded as a function of time, either of metal nanoparticles alone, or for a mixture of nanoparticles and lipidic nanovesicles. Lipid nanovesicles markedly decrease the NPs tendency to sediment irrespective of size or lipid compositions, thus keeping the metal oxide NPs in suspension. Cryo-electron microscopy, fluorescence anisotropy of TMA-DPH and general polarization of laurdan failed to reveal any major effect of the NPs on the lipid bilayer structure or phase state of the lipids. The above data may help in developing studies of the interaction of inhaled particles with lung surfactant lipids and alveolar macrophages.
authors: Unidad de Biofísica (CSIC, UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, P.O. Box 644, 48080 Bilbao, Spain.
authors: Noemi
authors: N
authors: Jiménez-Rojo
authors: Unidad de Biofísica (CSIC, UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, P.O. Box 644, 48080 Bilbao, Spain.
authors: Marta G
authors: MG
authors: Lete
authors: Biosurfaces Unit, Laboratory 1, Center for Cooperative Research in Biomaterials CICbiomagune, 20009 Donostia, Spain.
authors: Elena
authors: E
authors: Rojas
authors: Structural Biology Unit, Center for Cooperative Research in Biosciences, CICbiogune, 48160 Derio, Spain.
authors: David
authors: D
authors: Gil
authors: Structural Biology Unit, Center for Cooperative Research in Biosciences, CICbiogune, 48160 Derio, Spain.
authors: Mikel
authors: M
authors: Valle
authors: Unidad de Biofísica (CSIC, UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, P.O. Box 644, 48080 Bilbao, Spain.
authors: Alicia
authors: A
authors: Alonso
authors: Biosurfaces Unit, Laboratory 1, Center for Cooperative Research in Biomaterials CICbiomagune, 20009 Donostia, Spain.
authors: Sergio E
authors: SE
authors: Moya
authors: Unidad de Biofísica (CSIC, UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, P.O. Box 644, 48080 Bilbao, Spain. Electronic address: felix.goni@ehu.eus.
authors: Félix M
authors: FM
authors: Goñi
mesh_terms: D002563:Cerium / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009391:Nephelometry and Turbidimetry; D014025:Titanium / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D008081:Liposomes; C009495:titanium dioxide; D002563:Cerium; C030583:ceric oxide; D014025:Titanium; D015034:Zinc Oxide
keywords: Lipid nanovesicles; Liposomes; Lung surfactant; Membrane biophysics; Metal oxide nanoparticles