title: CDK14 Contributes to Reactive Gliosis via Interaction with Cyclin Y in Rat Model of Spinal Cord Injury.
abstract: Cyclin-dependent kinases (CDKs) are perceived as the engine that drives cell cycle progression whereas cyclins are considered to be the gears that are changed to aid the transition between cycle phases. CDK14 is a cdc2-related serine/threonine protein kinase and plays an important role in normal cell cycle progression. However, its distribution and function in the central nervous system (CNS) lesion remain unclear. In this study, we mainly investigated the protein expression and cellular localization of CDK14 during spinal cord injury (SCI). Western blot analysis revealed that the expression of CDK14 was gradually increased and reached a peak at 3 days after SCI. The expression of CDK14 was further analyzed by immunohistochemistry. Double immunofluorescence staining showed that CDK14 was co-expressed prominent in astrocytes. Co-localization CDK14/proliferating cell nuclear antigen (PCNA) were detected in glial cells. cyclin Y, which can interact with CDK14, was detected that had same expression trend was consistent with CDK14 Western blot results in SCI. Double-immunofluorescence staining indicated that CDK14 co-expressed with cyclin Y in some cells. Co-immunoprecipitation had been showed that CDK14 could interact with cyclin Y after acute SCI. Taken together, these data suggested that both CDK14 and cyclin Y may play important roles in spinal cord pathophysiology.
authors: Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors: Chengwei
authors: C
authors: Duan
authors: Department of Pathogen Biology, Medical College, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors: Yonghua
authors: Y
authors: Liu
authors: Department of Histology and Embryology, Medical College, Nantong University, Nantong, 226001, Jiangsu Province, China.
authors: Lu
authors: L
authors: Lu
authors: Department of Histology and Embryology, Medical College, Nantong University, Nantong, 226001, Jiangsu Province, China.
authors: Rixin
authors: R
authors: Cai
authors: Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors: Huaqing
authors: H
authors: Xue
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors: Xingxing
authors: X
authors: Mao
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors: Chen
authors: C
authors: Chen
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors: Rong
authors: R
authors: Qian
authors: Department of Pathogen Biology, Medical College, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China. zdm@ntu.edu.cn.
authors: Dongmei
authors: D
authors: Zhang
authors: Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China. shen_aiguo@yahoo.com.
authors: Aiguo
authors: A
authors: Shen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism*; D005911:Gliosis; D008297:Male; D009457:Neuroglia / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*
chemical_list: D016213:Cyclins; D018844:Cyclin-Dependent Kinases
keywords: CDK14; Cyclin Y; Spinal cord injury