title: Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase.
abstract: The present study focuses on the potential of glucose oxidase (GOD) as a promising biocontrol agent for fungal plant pathogens. In fact, a new GOD producing fungus was isolated and identified as an Aspergillus tubingensis. GOD (125 AU) has been found to inhibit Fusarium solani growth and spore production. Indeed, GOD caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. In vivo assays, GOD acted as a curative treatment capable of protecting the tomato plants against F. solani diseases. In fact, the incidence was null in the curative treatment with GOD and it is around 45% for the preventive treatment. The optimization of media composition and culture conditions led to a 2.6-fold enhancement in enzyme activity, reaching 81.48U/mL. This study has demonstrated that GOD is a potent antifungal agent that could be used as a new biofungicide to protect plants from diseases.
authors: Laboratoire de microorganismes et de biomolécules, Centre de biotechnologie de Sfax, Université de Sfax, route de Sidi Mansour km 6, BP 1177, Sfax, Tunisia.
authors: Mouna
authors: M
authors: Kriaa
authors: École supérieure d'agriculture du Kef, Université de Jendouba, Boulifa, 7119 Le Kef, Tunisia.
authors: Inès
authors: I
authors: Hammami
authors: Laboratoire de microorganismes et de biomolécules, Centre de biotechnologie de Sfax, Université de Sfax, route de Sidi Mansour km 6, BP 1177, Sfax, Tunisia.
authors: Mouna
authors: M
authors: Sahnoun
authors: Institut de l'Olivier, Université de Sfax, route de l'Aéroport km 2, 3000 Sfax, Tunisia.
authors: Manel Cheffi
authors: MC
authors: Azebou
authors: Institut de l'Olivier, Université de Sfax, route de l'Aéroport km 2, 3000 Sfax, Tunisia.
authors: Mohamed Ali
authors: MA
authors: Triki
authors: Laboratoire de microorganismes et de biomolécules, Centre de biotechnologie de Sfax, Université de Sfax, route de Sidi Mansour km 6, BP 1177, Sfax, Tunisia; Institut supérieur de biotechnologie de Sfax, route de Sokra km 3, BP 261, 3000 Sfax, Tunisia. Electronic address: radhouan.kammoun@cbs.rnrt.tn.
authors: Radhouane
authors: R
authors: Kammoun
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002119:Calcium Carbonate / Q000494:pharmacology; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004790:Enzyme Induction / Q000187:drug effects; D005656:Fungal Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects* / Q000502:physiology; D018525:Germination / Q000187:drug effects; D005949:Glucose Oxidase / Q000096:biosynthesis / Q000494:pharmacology*; D007218:Industrial Microbiology / Q000379:methods; D018551:Solanum lycopersicum / Q000382:microbiology*; D025282:Mycelium / Q000648:ultrastructure; D009584:Nitrogen / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology / Q000628:therapy*; D018517:Plant Roots / Q000382:microbiology; D013172:Spores, Fungal / Q000187:drug effects; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D002244:Carbon; D005949:Glucose Oxidase; D002119:Calcium Carbonate; D009584:Nitrogen
keywords: Antifungal activity; Aspergillus tubingensis; Biological control; Glucose oxidase; Optimization; Tomatoes