title: Comparative Assessment of Oral Mesenchymal Stem Cells Isolated from Healthy and Diseased Tissues.
abstract: The aim of the present study was to isolate human mesenchymal stem cells (MSCs) from palatal connective and periodontal granulation tissues and to comparatively evaluate their properties. MSCs were isolated using the explant culture method. Adherence to plastic, specific antigen makeup, multipotent differentiation potential, functionality, and ultrastructural characteristics were investigated. The frequency of colony-forming unit fibroblasts for palatal-derived mesenchymal stem cells (pMSCs) was significantly higher than that of granulation tissue-derived mesenchymal stem cells (gtMSCs). A significantly higher population doubling time and lower migration potential were recorded for gtMSCs than for pMSCs. Both cell lines were positive for CD105, CD73, CD90, CD44, and CD49f, and negative for CD34, CD45, and HLA-DR, but the level of expression was different. MSCs from both sources were relatively uniform in their ultrastructure. Generally, both cell lines possessed a large, irregular-shaped euchromatic nucleus, and cytoplasm rich in mitochondria, lysosomes, and endoplasmic reticulum. The periphery of the plasma membrane displayed many small filopodia. MSCs from both cell lines were successfully differentiated into osteogenic, adiopogenic, and chondrogenic lineages. Both healthy and diseased tissues may be considered as valuable sources of MSCs for regenerative medicine owing to the high acceptance and fewer complications during harvesting.
authors: 1Department of Reproduction,Obstetrics and Veterinary Gynecology, Faculty of Veterinary Medicine,University of Agricultural Sciences and Veterinary Medicine,3-5 Mănăştur St.,400372 Cluj-Napoca,Romania.
authors: Emöke
authors: E
authors: Páll
authors: 3Department of Cell and Molecular Biology, Faculty of Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 L. Pasteur St.,400349 Cluj-Napoca,Romania.
authors: Adrian
authors: A
authors: Florea
authors: 4Laboratory of Radiotherapy, Tumor and Radiobiology,Prof. Dr. "Ion Chiricuţă" Oncology Institute,34-36 I. Creangă St.,400015 Cluj-Napoca,Romania.
authors: Olga
authors: O
authors: Soriţău
authors: 1Department of Reproduction,Obstetrics and Veterinary Gynecology, Faculty of Veterinary Medicine,University of Agricultural Sciences and Veterinary Medicine,3-5 Mănăştur St.,400372 Cluj-Napoca,Romania.
authors: Mihai
authors: M
authors: Cenariu
authors: 2Department of Periodontology, Faculty of Dental Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,15 V. Babeş St.,400012 Cluj-Napoca,Romania.
authors: Adrian S
authors: AS
authors: Petruţiu
authors: 2Department of Periodontology, Faculty of Dental Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,15 V. Babeş St.,400012 Cluj-Napoca,Romania.
authors: Alexandra
authors: A
authors: Roman
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003238:Connective Tissue; D020869:Gene Expression Profiling; D006684:HLA-DR Antigens / Q000032:analysis; D064368:Healthy Volunteers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D008853:Microscopy; D009061:Mouth Mucosa / Q000166:cytology*; D015388:Organelles / Q000648:ultrastructure; D010510:Periodontal Diseases; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens
keywords: differentiation; granulation tissue; mesenchymal stem cells; palate; ultrastructure