title: Reconstituted Thymus Organ Culture.
abstract: Reconstituted thymus organ culture is based on fetal thymus organ culture (FTOC). Purified thymocyte populations, from genetically modified mice or even from other species, are cultured in vitro with thymic lobes depleted of their endogenous thymocytes (by 2'-deoxyguanosine treatment) to form a new thymus. This potent and timesaving method is distinct from FTOC, which assesses development of unmodified thymic lobes, and reaggregate thymic organ culture, in which epithelial cells are separately purified before being aggregated with thymocytes.
authors: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai, 200031, China.
authors: Zimu
authors: Z
authors: Deng
authors: Haifeng
authors: H
authors: Liu
authors: Jinxiu
authors: J
authors: Rui
authors: Xiaolong
authors: X
authors: Liu
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation; D005260:Female; D005333:Fetus; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D009924:Organ Culture Techniques; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012190:Retroviridae / Q000235:genetics; D060168:Thymocytes / Q000166:cytology / Q000378:metabolism; D013950:Thymus Gland; D014161:Transduction, Genetic