title: Elevated expression of T-bet in mycobacterial antigen-specific CD4(+) T cells from patients with tuberculosis.
abstract: T-bet is a T-box transcriptional factor that controls the differentiation and effector functions of CD4 T cells. In this study, we studied the role of T-bet in regulating CD4(+) T cell immunity against tuberculosis (TB). T-bet expression in Mycobacterium tuberculosis antigen-specific CD4(+) T cells was significantly higher in patients with active TB than in individuals with latent TB infection (p<0.0001). Comparison of T-bet expression in TCM and TEM subsets showed that CD4(+)T-bet(+)M. tuberculosis antigen-specific CD4(+) T cells had significantly lower frequency of TCM (p=0.003) and higher frequency of TEM (p=0.003) than CD4(+)T-bet(-) cells. The expression of PD-1 in antigen-specific CD4(+) T cells was significantly higher in patients with TB than in individuals with latent TB infection (p=0.006). CD4(+)CD154(+)T-bet(+) T cells had significantly higher expression of PD-1 than CD4(+)CD154(+)T-bet(-) T cells (p=0.0028). It is concluded that T-bet expression might be associated with differentiation into effector memory cells and PD-1 expression in mycobacterial antigen-specific CD4(+) T cells.
authors: Key Laboratory of Tuberculosis Prevention and Treatment and Beijing Key Laboratory of New Techniques for Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis, 309th Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Bingfen
authors: B
authors: Yang
authors: Key Laboratory of Tuberculosis Prevention and Treatment and Beijing Key Laboratory of New Techniques for Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis, 309th Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Fei
authors: F
authors: Zhai
authors: Key Laboratory of Tuberculosis Prevention and Treatment and Beijing Key Laboratory of New Techniques for Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis, 309th Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Jing
authors: J
authors: Jiang
authors: Key Laboratory of Tuberculosis Prevention and Treatment and Beijing Key Laboratory of New Techniques for Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis, 309th Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Xinjing
authors: X
authors: Wang
authors: Key Laboratory of Tuberculosis Prevention and Treatment and Beijing Key Laboratory of New Techniques for Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis, 309th Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Zhihong
authors: Z
authors: Cao
authors: Key Laboratory of Tuberculosis Prevention and Treatment and Beijing Key Laboratory of New Techniques for Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis, 309th Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China. Electronic address: xcheng79@outlook.com.
authors: Xiaoxing
authors: X
authors: Cheng
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D023201:CD40 Ligand / Q000276:immunology; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D055985:Latent Tuberculosis / Q000276:immunology* / Q000378:metabolism; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology; D061026:Programmed Cell Death 1 Receptor / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: D000942:Antigens, Bacterial; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D023201:CD40 Ligand; D007371:Interferon-gamma
keywords: CD4 T cells; T-bet; Tuberculosis