title: Best single-slice location to measure visceral adipose tissue on paediatric CT scans and the relationship between anthropometric measurements, gender and VAT volume in children.
abstract: OBJECTIVE
  Visceral adipose tissue (VAT) is a significant risk factor for obesity-related metabolic diseases. This study investigates (1) the best single CT slice location for predicting total abdominal VAT volume in paediatrics and (2) the relationship between waist circumference (WC), sagittal diameter (SD), gender and VAT volume.
  
  
  METHODS
  A random sample of 130 paediatric abdomen CT scans, stratified according to age and gender, was collected. Three readers measured VAT area at each intervertebral level between T12 and S1 using ImageJ analysis (National Institute of Health, Bethesda, MD) software by thresholding -190 to -30 HU and manually segmenting VAT. Single-slice VAT measurements were correlated with total VAT volume to identify the most representative slice. WC and SD were measured at L3-L4 and L4-L5 slices, respectively. Regression analysis was used to evaluate WC, SD and gender as VAT volume predictors.
  
  
  RESULTS
  Interviewer and intraviewer reliability were excellent (intraclass correlation coefficient = 0.99). Although VAT measured at multiple slices correlated strongly with abdominal VAT, only one slice in females at L2-L3 and two slices in males at L1-L2 and L5-S1 were strongly correlated across all age groups. Linear regression analysis showed that WC was strongly correlated with VAT volume (beta = 0.970, p < 0.001).
  
  
  CONCLUSIONS
  Single-slice VAT measurements are highly reproducible. Measurements performed at L2-L3 in females and L1-L2 or L5-S1 in males were most representative of VAT. WC is indicative of VAT.
  
  
  CONCLUSIONS
  VAT should be measured at L2-L3 in female children and at either L1-L2 or L5-S1 in males. WC is a strong indicator of VAT in children.
authors: School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors: Michelle
authors: M
authors: O'Connor
authors: School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors: John
authors: J
authors: Ryan
authors: School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors: Shane
authors: S
authors: Foley
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D050152:Intra-Abdominal Fat / Q000000981:diagnostic imaging*; D008297:Male; D015203:Reproducibility of Results; D012737:Sex Factors; D014057:Tomography, X-Ray Computed; D055105:Waist Circumference / Q000502:physiology*