title: [Effects of dexamethasone and superoxide dismutase instillations on clinical course of uveitis and local biochemical processes (experimental study)].
abstract: OBJECTIVE
  to evaluate and compare the effect of topical superoxide dismutase (SOD), which is an antioxidant enzyme, dexamethasone, and a combination of these on the course of experimental uveitis in rabbits as well as biochemical parameters of aqueous and vitreous humor.
  
  
  METHODS
  Acute uveitis was induced in 16 rabbits by a double injection (subcutaneous and intravitreal) of normal horse serum. Of them 12 animals, divided into 3 groups of 4 each, received topical SOD, dexamethasone, or both daily for 7 days. The remaining 4 rabbits (8 eyes) were treated with placebo and, thus, constituted the control group. On day 8 the following parameters were measured in aqueous humor: protein concentration, antioxidant activity, SOD activity, α2-macroglobulin level, and leukocyte number. Total protein and albumin levels in vitreous humor were also determined.
  
  
  RESULTS
  The effects of SOD and dexamethasone instillations were considered similar in many parameters. However, SOD was associated with a greater increase in antioxidant activity and a greater decrease in aqueous humor leukocytes, while dexamethasone was more effective in decreasing aqueous humor α2-macroglobulin and vitreous humor protein and albumin. The substances had a synergistic effect on iridal edema as well as aqueous humor leukocyte number and α2-macroglobulin level. CONCLUSION. Adding SOD to the complex therapy of uveitis results in lower inflammation intensity and enhanced dexamethasone effect.
authors: Moscow Helmholtz Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, 14/19 Sadovaya-Chernogryazskaya St., Moscow, Russian Federation, 105062.
authors: N B
authors: NB
authors: Chesnokova
authors: Moscow Helmholtz Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, 14/19 Sadovaya-Chernogryazskaya St., Moscow, Russian Federation, 105062.
authors: V V
authors: VV
authors: Neroev
authors: Moscow Helmholtz Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, 14/19 Sadovaya-Chernogryazskaya St., Moscow, Russian Federation, 105062.
authors: O V
authors: OV
authors: Beznos
authors: Moscow Helmholtz Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, 14/19 Sadovaya-Chernogryazskaya St., Moscow, Russian Federation, 105062.
authors: G A
authors: GA
authors: Beyshenova
authors: Koltzov Institute of Developmental Biology, 26 Vavilova st., Moscow, Russian Federation, 119334.
authors: I G
authors: IG
authors: Panova
authors: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina St., Moscow, Russian Federation, 119334.
authors: A S
authors: AS
authors: Tatikolov
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects / Q000378:metabolism*; D001669:Biochemical Phenomena / Q000187:drug effects; D003907:Dexamethasone / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D016166:Free Radical Scavengers / Q000008:administration & dosage; D005938:Glucocorticoids / Q000008:administration & dosage; D007322:Instillation, Drug; D011817:Rabbits; D013482:Superoxide Dismutase / Q000008:administration & dosage*; D014605:Uveitis / Q000175:diagnosis / Q000188:drug therapy*; D014822:Vitreous Body / Q000187:drug effects / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D005938:Glucocorticoids; D003907:Dexamethasone; D013482:Superoxide Dismutase