title: Schmallenberg virus: experimental infection in goats and bucks.
abstract: BACKGROUND
  Schmallenberg virus (SBV) is an emerging Orthobunyavirus of ruminant livestock species currently circulating in Europe. SBV causes a subclinical or mild disease in adult animals but vertical transmission to pregnant dams may lead to severe malformations in the offspring. Data on the onset of clinical signs, viremia and seroconversion in experimentally infected adult animals are available for cattle and sheep but are still lacking for goats. For a better understanding of the pathogenesis of SBV infection in adult ruminants, we carried out experimental infections in adult goats. Our specific objectives were: (i) to record clinical signs, viremia and seroconversion; (ii) to monitor viral excretion in the semen of infected bucks; (iii) to determine in which tissues SBV replication took place and virus-induced lesions developed.
  
  
  RESULTS
  Four goats and two bucks were inoculated with SBV. Virus inoculation was followed by a short viremic phase lasting 3 to 4 days and a seroconversion occurring between days 7 and 14 pi in all animals. The inoculated goats did not display any clinical signs, gross lesions or histological lesions. Viral genomic RNA was found in one ovary but could not be detected in other organs. SBV RNA was not found in the semen samples collected from two inoculated bucks.
  
  
  CONCLUSIONS
  In the four goats and two bucks, the kinetics of viremia and seroconversion appeared similar to those previously described for sheep and cattle. Our limited set of data provides no evidence of viral excretion in buck semen.
authors: Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, Unité d'anatomie pathologique, 7 avenue du Général de Gaulle, 94704, Maisons-Alfort, France. elaloy@vet-alfort.fr.
authors: E
authors: E
authors: Laloy
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. Mickael.Riou@tours.inra.fr.
authors: M
authors: M
authors: Riou
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. Celine.Barc@tours.inra.fr.
authors: C
authors: C
authors: Barc
authors: Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, Unité de pathologie des animaux de production, 7 avenue du Général de Gaulle, 94704, Maisons-Alfort, France. gbelbis@vet-alfort.fr.
authors: G
authors: G
authors: Belbis
authors: ANSES, UMR 1161 Virologie ANSES-INRA-ENVA, 23 avenue du Général de Gaulle, 94704, Maisons-Alfort, France. emmanuel.breard@anses.fr.
authors: E
authors: E
authors: Bréard
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. Sylvain.Breton@tours.inra.fr.
authors: S
authors: S
authors: Breton
authors: Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, Unité d'anatomie pathologique, 7 avenue du Général de Gaulle, 94704, Maisons-Alfort, France. ncordonnier@vet-alfort.fr.
authors: N
authors: N
authors: Cordonnier
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. Didier.Crochet@tours.inra.fr.
authors: D
authors: D
authors: Crochet
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. Remi.Delaunay@tours.inra.fr.
authors: R
authors: R
authors: Delaunay
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. joel.moreau@tours.inra.fr.
authors: J
authors: J
authors: Moreau
authors: LNCR, Laboratoire national de contrôle des reproducteurs, 13 rue Jouët, 94703, Maisons-Alfort, France. nathalie.pozzi@lncr.org.
authors: N
authors: N
authors: Pozzi
authors: LNCR, Laboratoire national de contrôle des reproducteurs, 13 rue Jouët, 94703, Maisons-Alfort, France. maxime.raimbourg@lncr.org.
authors: M
authors: M
authors: Raimbourg
authors: INRA Centre Val de Loire, UE-1277 Plateforme d'Infectiologie Expérimentale, secteur 3, route de Crotelles, 37380, Nouzilly, France. pierre.sarradin@tours.inra.fr.
authors: P
authors: P
authors: Sarradin
authors: INRA Centre Val de Loire, UMR 1282 Infectiologie et Santé Publique, 37380, Nouzilly, France. sascha.trapp@tours.inra.fr.|Université François Rabelais de Tours, UMR 1282 Infectiologie et Santé Publique, 37000, Tours, France. sascha.trapp@tours.inra.fr.
authors: S
authors: S
authors: Trapp
authors: ANSES, UMR 1161 Virologie ANSES-INRA-ENVA, 23 avenue du Général de Gaulle, 94704, Maisons-Alfort, France. cyril.viarouge@anses.fr.
authors: C
authors: C
authors: Viarouge
authors: ANSES, UMR 1161 Virologie ANSES-INRA-ENVA, 23 avenue du Général de Gaulle, 94704, Maisons-Alfort, France. szientara@vet-alfort.fr.
authors: S
authors: S
authors: Zientara
authors: LNCR, Laboratoire national de contrôle des reproducteurs, 13 rue Jouët, 94703, Maisons-Alfort, France. claire.ponsart@anses.fr.
authors: C
authors: C
authors: Ponsart
mesh_terms: D000818:Animals; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008297:Male; D016851:Orthobunyavirus / Q000302:isolation & purification*; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D012367:RNA, Viral