title: An acidified thermostabilizing mini-peptide derived from the carboxyl extension of the larger isoform of the plant Rubisco activase.
abstract: Thermostable fusion peptide partners are valuable in engineering thermostability in proteins. We evaluated the Arabidopsis counterpart (AtRAce) and an acidified derivative (mRAce) of the conserved carboxyl extension (RAce) of plant Rubisco activase (RCA) for their thermostabilizing properties in Escherichia coli and Saccharomyces cerevisiae using a protein fusion strategy. We used AtRAce and mRAce as fusion tails for the thermolabile protein RCA2 from Arabidopsis thaliana and Nicotiana tabacum. The homologous fusion of AtRAce with Arabidopsis RCA2 and the heterologous fusion of AtRAce with tobacco RCA2 increased the thermostability of both proteins. The acidified derivative mRAce conferred greater thermostability upon both proteins as compared with AtRAce. Moreover, mRAce enhanced the thermostability of other two thermolabile proteins from Jatropha curcas: the cytosolic ascorbate peroxidase 1 (JcAPX1) and the TATA-box binding protein isoform 1 (JcTBP1). We further report - for the first time - that JcTBP1 mediates heat tolerance in vivo in yeast. Thus, our study identifies a C-terminal acidic mini-peptide - the acidified derivative mRAce - with potential uses in improving the thermostability of heat-labile proteins and their associated heat tolerance in host organisms.
authors: School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming 650500, Yunnan, China.
authors: Mengru
authors: M
authors: Zhang
authors: Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661600, Yunnan, China.
authors: Xujuan
authors: X
authors: Li
authors: School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming 650500, Yunnan, China.
authors: Yumei
authors: Y
authors: Yang
authors: School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming 650500, Yunnan, China.
authors: Zhu
authors: Z
authors: Luo
authors: School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming 650500, Yunnan, China.
authors: Chang
authors: C
authors: Liu
authors: School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming 650500, Yunnan, China. Electronic address: gongming63@163.com.
authors: Ming
authors: M
authors: Gong
authors: School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming 650500, Yunnan, China. Electronic address: zzr09@sina.com.
authors: Zhurong
authors: Z
authors: Zou
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000235:genetics*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D031286:Jatropha / Q000378:metabolism; D010455:Peptides / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D035181:TATA-Box Binding Protein / Q000235:genetics*; D014026:Nicotiana / Q000378:metabolism
chemical_list: D010455:Peptides; D010940:Plant Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D035181:TATA-Box Binding Protein; C054765:rca protein, plant; D060387:Ascorbate Peroxidases
keywords: Ascorbate peroxidase 1; Hyper-acidic fusion tail; Rubisco activase; TATA-box binding protein; Thermostabilizing