title: Accelerated Optical Projection Tomography Applied to In Vivo Imaging of Zebrafish.
abstract: Optical projection tomography (OPT) provides a non-invasive 3-D imaging modality that can be applied to longitudinal studies of live disease models, including in zebrafish. Current limitations include the requirement of a minimum number of angular projections for reconstruction of reasonable OPT images using filtered back projection (FBP), which is typically several hundred, leading to acquisition times of several minutes. It is highly desirable to decrease the number of required angular projections to decrease both the total acquisition time and the light dose to the sample. This is particularly important to enable longitudinal studies, which involve measurements of the same fish at different time points. In this work, we demonstrate that the use of an iterative algorithm to reconstruct sparsely sampled OPT data sets can provide useful 3-D images with 50 or fewer projections, thereby significantly decreasing the minimum acquisition time and light dose while maintaining image quality. A transgenic zebrafish embryo with fluorescent labelling of the vasculature was imaged to acquire densely sampled (800 projections) and under-sampled data sets of transmitted and fluorescence projection images. The under-sampled OPT data sets were reconstructed using an iterative total variation-based image reconstruction algorithm and compared against FBP reconstructions of the densely sampled data sets. To illustrate the potential for quantitative analysis following rapid OPT data acquisition, a Hessian-based method was applied to automatically segment the reconstructed images to select the vasculature network. Results showed that 3-D images of the zebrafish embryo and its vasculature of sufficient visual quality for quantitative analysis can be reconstructed using the iterative algorithm from only 32 projections-achieving up to 28 times improvement in imaging speed and leading to total acquisition times of a few seconds.
authors: Department of Computer Science, University College London, London, United Kingdom.
authors: Teresa
authors: T
authors: Correia
authors: Division of Medicine, University College London, London, United Kingdom; CoMPLEX, University College London, London, United Kingdom.
authors: Nicola
authors: N
authors: Lockwood
authors: Department of Physics, Imperial College London, London, United Kingdom.
authors: Sunil
authors: S
authors: Kumar
authors: Department of Computer Science, University College London, London, United Kingdom.
authors: Jun
authors: J
authors: Yin
authors: Division of Medicine, University College London, London, United Kingdom; Department of Life Sciences, Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom.
authors: Marie-Christine
authors: MC
authors: Ramel
authors: Department of Life Sciences, Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom.
authors: Natalie
authors: N
authors: Andrews
authors: Department of Structural and Molecular Biology, University College London, London, United Kingdom.
authors: Matilda
authors: M
authors: Katan
authors: Department of Life Sciences, Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom.
authors: Laurence
authors: L
authors: Bugeon
authors: Department of Life Sciences, Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom.
authors: Margaret J
authors: MJ
authors: Dallman
authors: Department of Physics, Imperial College London, London, United Kingdom.
authors: James
authors: J
authors: McGinty
authors: Division of Medicine, University College London, London, United Kingdom.
authors: Paul
authors: P
authors: Frankel
authors: Department of Physics, Imperial College London, London, United Kingdom.
authors: Paul M W
authors: PM
authors: French
authors: Department of Computer Science, University College London, London, United Kingdom.
authors: Simon
authors: S
authors: Arridge
mesh_terms: D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian / Q000098:blood supply*; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000662:veterinary*; D008027:Light; D013194:Staining and Labeling; D041622:Tomography, Optical / Q000379:methods / Q000662:veterinary*; D015027:Zebrafish / Q000033:anatomy & histology*