title: Immobilized soybean hull peroxidase for the oxidation of phenolic compounds in coffee processing wastewater.
abstract: Chitosan beads were prepared, using glutaraldehyde as a crosslinking agent for the immobilization of soybean hull peroxidase (SBP). The activity of free and immobilized SBP was studied. The optimum pH was 6.0 for both the free and immobilized enzyme; however, enzyme activity became more dependent on the temperature after immobilization. This study evaluated the potential use of immobilized and free enzyme in the oxidation of caffeic acid, of synthetic phenolic solution (SPS) and of total phenolic compounds in coffee processing wastewater (CPW). Some factors, such as reaction time, amount of H2O2 and caffeic acid were evaluated, in order to determine the optimum conditions for enzyme performance. Both enzymes showed a potential in the removal of caffeic acid, SPS and CPW, and immobilized SBP had the highest oxidation performance. The immobilized enzyme showed a potential of 50% in the oxidation of caffeic acid after 4 consecutive cycles.
authors: Laboratório de Bioquímica, Departamento de Química, Universidade Federal de Lavras, CEP 37200-000, Lavras, MG, Brazil. Electronic address: pricilambatista@yahoo.com.br.
authors: Pricila Maria Batista
authors: PM
authors: Chagas
authors: Laboratório de Bioquímica, Departamento de Química, Universidade Federal de Lavras, CEP 37200-000, Lavras, MG, Brazil.
authors: Juliana Arriel
authors: JA
authors: Torres
authors: Centro Federal de Educação Tecnológica de Minas Gerais, CEP 30421-169, Belo Horizonte, MG, Brazil.
authors: Maria Cristina
authors: MC
authors: Silva
authors: Laboratório de Bioquímica, Departamento de Química, Universidade Federal de Lavras, CEP 37200-000, Lavras, MG, Brazil. Electronic address: angelita@dqi.ufla.br.
authors: Angelita Duarte
authors: AD
authors: Corrêa
mesh_terms: D002109:Caffeic Acids / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D003069:Coffee / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005976:Glutaral; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D013025:Glycine max / Q000201:enzymology*; D013696:Temperature; D062065:Wastewater / Q000737:chemistry*
chemical_list: D002109:Caffeic Acids; D048271:Chitosan; D003069:Coffee; D004800:Enzymes, Immobilized; D005976:Glutaral; D010544:Peroxidases; D010636:Phenols; D062065:Wastewater; C040048:caffeic acid
keywords: Caffeic acid; Chitosan; Coffee processing wastewater; Enzyme; Glutaraldehyde; Immobilization