title: Analysis of malachite green in aquatic products by carbon nanotube-based molecularly imprinted - matrix solid phase dispersion.
abstract: A simple method based on matrix solid phase dispersion (MSPD) using molecularly imprinted polymers (MIPs) as sorbents for selective extraction of malachite green (MG) from aquatic products was developed. The MIPs were prepared by using carbon nanotube as support, MG as template, methacrylic acid as functional monomer, ethyleneglycol dimethacrylate as crosslinker and methylene chloride as solvent. The MIPs were characterized by Fourier transform infrared spectrometry and transmission electron microscopy. The isothermal adsorption, kinetics absorption and selective adsorption experiments were carried out. We optimized the extraction conditions as follows: the ratio of MIPs to sample was 2:3, the dispersion time was 15min, washing solvent was 4mL 50% aqueous methanol and elution solvent was 3mL methanol-acetic acid (98: 2, v/v). Once the MSPD process was completed, the MG extracted from aquatic products was determined by high performance liquid chromatography. The detection limit of MG was 0.7μgkg(-1). The relative standard deviations of intra-day and inter-day were obtained in the range of 0.9%-4.7% and 3.4%-9.8%, respectively. In order to evaluate the applicability and reliability of the proposed method, it was applied to determine MG in different aquatic products samples including fish, shrimp, squid and crabs. The satisfied recoveries were in the range of 89.2%-104.6%. The results showed that this method is faster, simpler and makes extraction and purification in the same system.
authors: Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Yu
authors: Y
authors: Wang
authors: Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. Electronic address: ligangchen2010@aliyun.com.
authors: Ligang
authors: L
authors: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003445:Crustacea / Q000737:chemistry*; D049832:Decapodiformes / Q000737:chemistry*; D005399:Fishes; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon; D012394:Rosaniline Dyes / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D037742:Nanotubes, Carbon; D012394:Rosaniline Dyes; C005095:malachite green
keywords: Carbon nanotube; High performance liquid chromatography; Malachite green; Matrix solid phase dispersion; Molecularly imprinted polymer