title: Altered hepatic mRNA expression of immune response-associated DNA damage in mice liver induced by potassium bromate: Protective role of vanillin.
abstract: Chronic exposure to potassium bromate (KBrO3), a toxic halogen existing widely in the environment, environment through contaminated drinking water, has become a global problem of public health. The present study investigates the protective role of vanillin against KBrO3induced oxidative stress, distruption in inflammatory cytokines expression, DNA damage, and histopathological changes. Adult mice were exposed orally to KBrO3(2g/L of drinking water) for 2 weeks The co-administration of vanillin to the KBrO3-treated mice significantly prevented the plasma transaminases increase in. Furthermore, it inhibited hepatic lipid peroxidation (malondialdehyde), advanced oxidation protein product (AOPP) and protein carbonyl (PCO) formation and attenuated the KBrO3-mediated depletion of enzymatic and non enzymatic antioxidants catalase, superoxide dismutase, and glutathione peroxidase activities and glutathione level in the liver. In addition, vanillin markedly attenuated the expression levels of proinflammatory cytokines, including tumor necrosis factor-α, interleukin-1β, interleukin-6, and COX2 and prevented KBrO3-induced hepatic cell alteration and necrosis, as indicated by histopathological data. DNA damage, as assessed by the alkaline comet assay, was also found to be low in the co-treated group. Thus, these findings show that vanillin acts as potent chemopreventive agent against KBrO3-mediated liver oxidative stress and genotoxicity through its antioxidant properties. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1796-1807, 2016.
authors: Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia.
authors: Hajer
authors: H
authors: Ben Saad
authors: Enzymes and Bioconversions Laboratory, National Engineering School, University of Sfax, BP 1173, 3038 Sfax, Tunisia.
authors: Dorra
authors: D
authors: Driss
authors: Higher Institute of Biotechnology of Sfax, University Tunisia, 3000 Sfax, Tunisia.
authors: Ibtissem
authors: I
authors: Ben Amara
authors: Anatomopathology Laboratory, Habib Bourguiba Hospital, University of Sfax, 3029 Sfax, Tunisia.
authors: Ons
authors: O
authors: Boudawara
authors: Anatomopathology Laboratory, Habib Bourguiba Hospital, University of Sfax, 3029 Sfax, Tunisia.
authors: Tahia
authors: T
authors: Boudawara
authors: Enzymes and Bioconversions Laboratory, National Engineering School, University of Sfax, BP 1173, 3038 Sfax, Tunisia.
authors: Samia
authors: S
authors: Ellouz Chaabouni
authors: Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia.
authors: Khaled
authors: K
authors: Mounir Zeghal
authors: Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia.
authors: Ahmed
authors: A
authors: Hakim
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001547:Benzaldehydes / Q000494:pharmacology*; D001959:Bromates / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D062107:Advanced Oxidation Protein Products; D000975:Antioxidants; D001547:Benzaldehydes; D001959:Bromates; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D014874:Water Pollutants, Chemical; C019536:potassium bromate; C100058:vanillin; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: genes expression; genotoxicity; oxidative stress; potassium bromate; vanillin