title: Subcellular distribution and uptake mechanism of di-n-butyl phthalate in roots of pumpkin (Cucurbita moschata) seedlings.
abstract: Phthalate acid esters (PAEs) are of particular concern due to their potential environmental risk to human and nonhuman organisms. Although uptake of PAEs by plants has been reported by several researchers, information about the intracellular distribution and uptake mechanisms of PAEs is still lacking. In this study, a series of hydroponic experiments using intact pumpkin (Cucurbita moschata) seedlings was conducted to investigate how di-n-butyl phthalate (DnBP), one of the most frequently identified PAEs in the environment, enters and is distributed in roots. DnBP was transported into subcellular tissues rapidly in the initial uptake period (<12 h). More than 80% of DnBP was detected in the cell walls and organelles, which suggests that DnBP is primarily accumulated in these two fractions due to their high affinity to DnBP. The kinetics of DnBP uptake were fitted well with the Michaelis-Menten equation, suggesting that a carrier-mediated process was involved. The application of 2,4-dinitrophenol and sodium vanadate reduced the uptake of DnBP by 37 and 26%, respectively, while aquaporin inhibitors, silver and glycerol, had no effect on DnBP uptake. These data demonstrated that the uptake of DnBP included a carrier-mediated and energy-dependent process without the participation of aquaporins.
authors: School of Environmental Science and Engineering, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China.
authors: Qingqi
authors: Q
authors: Lin
authors: Experimental Teaching Center, Sun Yat-sen University, Zhuhai Campus, Tangjiawan Town, Zhuhai, 519082, China.
authors: Xiuhong
authors: X
authors: Yang
authors: School of Environmental Science and Engineering, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China.|Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China.
authors: Xiongfei
authors: X
authors: Huang
authors: School of Environmental Science and Engineering, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China. wshizh2@mail.sysu.edu.cn.|Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China. wshizh2@mail.sysu.edu.cn.
authors: Shizhong
authors: S
authors: Wang
authors: School of Environmental Science and Engineering, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China.|Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China.
authors: Yuanqing
authors: Y
authors: Chao
authors: School of Environmental Science and Engineering, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China. eesqrl@mail.sysu.edu.cn.|Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, D604, Dihuan Building, 135 Xingang Xi Road, Guangzhou, 510275, China. eesqrl@mail.sysu.edu.cn.
authors: Rongliang
authors: R
authors: Qiu
mesh_terms: D028464:Cucurbita / Q000378:metabolism*; D003993:Dibutyl Phthalate / Q000378:metabolism*; D006801:Humans; D018517:Plant Roots / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism
chemical_list: D003993:Dibutyl Phthalate
keywords: Di-n-butyl phthalate (DnBP); Pumpkin (Cucurbita moschata); Subcellular distribution; Uptake mechanism