title: The DEK Oncoprotein Is a Critical Component of the EKLF/KLF1 Enhancer in Erythroid Cells.
abstract: Understanding how transcriptional regulators are themselves controlled is important in attaining a complete picture of the intracellular effects that follow signaling cascades during early development and cell-restricted differentiation. We have addressed this issue by focusing on the regulation of EKLF/KLF1, a zinc finger transcription factor that plays a necessary role in the global regulation of erythroid gene expression. Using biochemical affinity purification, we have identified the DEK oncoprotein as a critical factor that interacts with an essential upstream enhancer element of the EKLF promoter and exerts a positive effect on EKLF levels. This element also binds a core set of erythroid transcription factors, suggesting that DEK is part of a tissue-restricted enhanceosome that contains BMP4-dependent and -independent components. Together with local enrichment of properly coded histones and an open chromatin domain, optimal transcriptional activation of the EKLF locus can be established.
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.
authors: Felix
authors: F
authors: Lohmann
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.
authors: Mohan
authors: M
authors: Dangeti
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.
authors: Shefali
authors: S
authors: Soni
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.
authors: Xiaoyong
authors: X
authors: Chen
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.
authors: Antanas
authors: A
authors: Planutis
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA.
authors: Margaret H
authors: MH
authors: Baron
authors: Department of Pathology, Washington University School of Medicine, St. Louis, Missouri, USA.
authors: Kyunghee
authors: K
authors: Choi
authors: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA james.bieker@mssm.edu.
authors: James J
authors: JJ
authors: Bieker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D041905:Erythroid Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000032:analysis / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D055415:Bone Morphogenetic Protein 4; D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D051741:Kruppel-Like Transcription Factors; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D011994:Recombinant Proteins; C080774:erythroid Kruppel-like factor