title: Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model.
abstract: OBJECTIVE
  To evaluate the ability of the glycolytic enzyme alpha-enolase (ENO1) or its immunodominant peptide (pEP1) to reduce the severity of CIA in DBA/1 mice when injected in a prophylactic way.
  
  
  METHODS
  Mice were treated with mouse ENO1 or pEP1 one day prior to collagen II immunization. Clinical assessment was evaluated using 4 parameters (global and articular scores, ankle thickness and weight). Titers of serum anti-ENO1, anti-cyclic citrullinated peptides (anti-CCP) and anti-CII (total IgG and IgG1/IgG2a isotypes) antibodies were measured by ELISA at different time-points. Disease activity was assessed by histological analysis of both anterior and hind paws at the end of experimentation.
  
  
  RESULTS
  Prophylactic injection of 100 μg of ENO1 reduced severity of CIA. Serum levels of anti-CII antibodies were reduced in ENO1-treated mice. Concordantly, ENO1-treated mice joints presented less severe histological signs of arthritis. ENO1 did not induce a shift toward a Th2 response since IgG1/IgG2a ratio of anti-CII antibodies remained unchanged and IL-4 serum levels were similar to those measured in the control group.
  
  
  CONCLUSIONS
  Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels. Effects of pEP1 were less pronounced. This immunomodulatory effect is associated with a reduction in anti-CII antibodies production but is not due to a Th1/Th2 shift.
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
authors: Clément
authors: C
authors: Guillou
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
authors: Céline
authors: C
authors: Derambure
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
authors: Manuel
authors: M
authors: Fréret
authors: Rouen University Hospital, Department of Rheumatology, Rouen, France.
authors: Mathieu
authors: M
authors: Verdet
authors: Rouen University Hospital, Department of Rheumatology, Rouen, France.
authors: Gilles
authors: G
authors: Avenel
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
authors: Marie-Laure
authors: ML
authors: Golinski
authors: Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Rouen University Hospital, Department of Pathology, Rouen, France.
authors: Jean-Christophe
authors: JC
authors: Sabourin
authors: Rouen University Hospital, Department of Pathology, Rouen, France.
authors: François
authors: F
authors: Le Loarer
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
authors: Sahil
authors: S
authors: Adriouch
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Rouen University Hospital, Department of Immunology, Rouen, France.
authors: Olivier
authors: O
authors: Boyer
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Rouen University Hospital, Department of Rheumatology, Rouen, France.
authors: Thierry
authors: T
authors: Lequerré
authors: INSERM, U905, Rouen, France; Normandy University, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Rouen University Hospital, Department of Rheumatology, Rouen, France.
authors: Olivier
authors: O
authors: Vittecoq
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology / Q000628:therapy*; D001323:Autoantibodies / Q000008:administration & dosage / Q000097:blood; D004195:Disease Models, Animal; D006801:Humans; D016056:Immunodominant Epitopes / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007596:Joints / Q000187:drug effects / Q000473:pathology; D051379:Mice; D010455:Peptides / Q000008:administration & dosage*; D010456:Peptides, Cyclic / Q000097:blood*; D010751:Phosphopyruvate Hydratase / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology
chemical_list: D001323:Autoantibodies; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D010455:Peptides; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide; C457379:Eno1 protein, mouse; D010751:Phosphopyruvate Hydratase