title: Anti-adipogenic and antioxidant effects of the traditional Korean herbal formula Samchulgeonbi-tang: an in vitro study.
abstract: OBJECTIVE
  Here we report in vitro anti-adipogenic and antioxidant effects of Samchulgeonbi-tang (SCGBT), a traditional Korean herbal formula.
  
  
  METHODS
  3T3-L1 preadipocytes were differentiated into adipocytes with or without SCGBT. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity and leptin production. In addition, its effect on scavenging activities of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals in in vitro systems.
  
  
  RESULTS
  In differentiated 3T3-L1 adipocytes, SCGBT significantly inhibited lipid accumulation and triglyceride production, and mediated inactivation of GPDH, a major enzyme in the process of adipogenesis. Consistent with this, SCGBT stimulation significantly decreased the amount of leptin in 3T3-L1 adipose cells. Furthermore, SCGBT enhanced the scavenging activities on ABTS and DPPH radicals. The generation of malondialdehyde (MDA) during low-density lipoprotein (LDL) oxidation was significantly reduced by SCGBT treatment. Of interest, SCGBT extract inhibited reactive oxygen species (ROS) generation in 3T3-L1 adipocytes.
  
  
  CONCLUSIONS
  Overall, our findings suggest that SCGBT has the potential for anti-adipogenic activity and antioxidant properties.
authors: K-herb Research Center, Korea Institute of Oriental Medicine Daejeon, Republic of Korea.
authors: Sae-Rom
authors: SR
authors: Yoo
authors: K-herb Research Center, Korea Institute of Oriental Medicine Daejeon, Republic of Korea.
authors: Chang-Seob
authors: CS
authors: Seo
authors: KM Convergence Research Division, Korea Institute of Oriental Medicine Daejeon, Republic of Korea.
authors: Ohn-Soon
authors: OS
authors: Kim
authors: K-herb Research Center, Korea Institute of Oriental Medicine Daejeon, Republic of Korea.
authors: Hyeun-Kyoo
authors: HK
authors: Shin
authors: KM Convergence Research Division, Korea Institute of Oriental Medicine Daejeon, Republic of Korea.
authors: Soo-Jin
authors: SJ
authors: Jeong
keywords: Anti-adipogenic; Samchulgeonbi-tang; antioxidant; korean herbal formula