title: [Correlation of Plasma Co-stimulatory Molecules B7-H2 and B7-H3 with Platelet Auto-antibodies in Patients with Immune Thrombocytopenic Purpura].
abstract: OBJECTIVE
  To investigate whether the plasma level of platelet auto- antibodies in ITP patients is related to that of co-stimulatory molecules sB7-H2 and sB7-H3.
  
  
  METHODS
  A total of 61 ITP patients and 25 healthy controls from the First Affiliated Hospital of Soochow University from June 2012 to August 2013 were enrolled in this study. The expression levels of platelet auto-antibodies against 5 glycoproteins (GPIX, GP Ib, GP IIIa, GPIIb and P-selectin) in plasma were detected by flow cytometric immuno-beads array, and the expression of soluable co-stimulatory molecules sB7-H2 and sB7-H3 was measured by ELISA.
  
  
  RESULTS
  The plasma levels of 5 auto-antibodies against platelet membrance glycoproteins significantly increased in ITP patiens (P < 0.01). Compared with healthy controls, sB7-H2 levels increased (P < 0.05), while the sB7-H3 level did not significantly change (r = 0.13, P > 0.05). However, the correlation analysis showed that sB7-H3 negatively correlated with platelet P-selectin auto-antibody (r = -0.46, P < 0.05), and sB7-H2 and sB7-H3 significantly reduced in ITP patients with positive P-selectin auto-antibody (P < 0.01). In ITP patients, platelet counts negatively correlated with sB7-H2 (r = -0.3907, P < 0.01), but did not correlate with sB7-H3.
  
  
  CONCLUSIONS
  Soluble costimulatory molecule sB7-H2 elevates in ITP patients, and the level of sB7-H3 is associated with auto-antibodies against P-selectin, suggesting that costimulatory molecules B7-H2 and B7-H3 may be involved in the pathogenesis of immune regulation abnormality in ITP.
authors: MOE Engineering Center of Hematological Disease, Soochow University, Suzhou 215123, Jiangsu Province, China.
authors: Bin
authors: B
authors: Zuo
authors: Key Laboratiry of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Yun-Xiao
authors: YX
authors: Zhao
authors: MOE Engineering Center of Hematological Disease, Soochow University, Suzhou 215123, Jiangsu Province, China.
authors: Jian-Feng
authors: JF
authors: Yang
authors: MOE Engineering Center of Hematological Disease, Soochow University, Suzhou 215123, Jiangsu Province, China.|Key Laboratiry of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China. E-mail: heyang1963@163.com.
authors: Yang
authors: Y
authors: He
mesh_terms: D001323:Autoantibodies; D060887:B7 Antigens; D001792:Blood Platelets; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D010980:Platelet Membrane Glycoproteins; D016553:Purpura, Thrombocytopenic, Idiopathic
chemical_list: D001323:Autoantibodies; D060887:B7 Antigens; D010980:Platelet Membrane Glycoproteins