title: A Crimean-Congo hemorrhagic fever (CCHF) viral vaccine expressing nucleoprotein is immunogenic but fails to confer protection against lethal disease.
abstract: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne disease, endemic in many countries in Africa, the Middle East, Eastern Europe and Asia. Between 15-70% of reported cases are fatal with no approved vaccine available. In the present study, the attenuated poxvirus vector, Modified Vaccinia virus Ankara, was used to develop a recombinant candidate vaccine expressing the CCHF virus nucleoprotein. Cellular and humoral immunogenicity was confirmed in 2 mouse strains, including type I interferon receptor knockout mice, which are susceptible to CCHF disease. Despite the immune responses generated post-immunisation, the vaccine failed to protect animals from lethal disease in a challenge model.
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: S D
authors: SD
authors: Dowall
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: K R
authors: KR
authors: Buttigieg
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: S J D
authors: SJ
authors: Findlay-Wilson
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: E
authors: E
authors: Rayner
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: G
authors: G
authors: Pearson
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: A
authors: A
authors: Miloszewska
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: V A
authors: VA
authors: Graham
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: M W
authors: MW
authors: Carroll
authors: a Public Health England ; Porton Down; Salisbury , Wiltshire , UK.
authors: R
authors: R
authors: Hewson
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology*; D006479:Hemorrhagic Fever, Crimean / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007114:Immunization; D000071497:Immunogenicity, Vaccine / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D009698:Nucleoproteins / Q000276:immunology*; D053633:Receptor, Interferon alpha-beta / Q000235:genetics; D014614:Vaccines, Synthetic / Q000276:immunology*; D014709:Vero Cells; D019562:Viral Load / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D009698:Nucleoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D053633:Receptor, Interferon alpha-beta
keywords: Crimean-Congo hemorrhagic fever, nucleoprotein, protection, vaccine