title: miR-526b targets 3' UTR of MMP1 mRNA.
abstract: Regulation of matrix metalloproteinases (MMPs) is important for many physiological processes involving cancers, inflammation, tissue remodeling and skin aging. Here, we report the novel finding that the expression of MMP1 mRNA is downregulated by the overexpression of miR-526b which is a member of chromosome 19 microRNA cluster (C19MC). Our analysis using reporter constructs containing the 3' untranslated region (3' UTR) of MMP1 and its mutant form showed that the region from 377-383 in the 3' UTR of MMP1 is critical for targeting by miR-526b. In addition, the expression pattern of miR-526b and MMP1 mRNA showed reverse relation between adult dermal and neonatal fibroblasts. We show for the first time that miR-526b, an miRNA belonging to C19MC, can target the 377-383 region of the MMP1 3' UTR.
authors: Bioscience Research Institute, AmorePacific Corporation R&D Center, Gyeonggi-do, Republic of Korea.
authors: Kyu-Han
authors: KH
authors: Kim
authors: Bioscience Research Institute, AmorePacific Corporation R&D Center, Gyeonggi-do, Republic of Korea.
authors: Ji-Yong
authors: JY
authors: Jung
authors: Bioscience Research Institute, AmorePacific Corporation R&D Center, Gyeonggi-do, Republic of Korea.
authors: Eui Dong
authors: ED
authors: Son
authors: Bioscience Research Institute, AmorePacific Corporation R&D Center, Gyeonggi-do, Republic of Korea.
authors: Dong Wook
authors: DW
authors: Shin
authors: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Republic of Korea.
authors: Minsoo
authors: M
authors: Noh
authors: Bioscience Research Institute, AmorePacific Corporation R&D Center, Gyeonggi-do, Republic of Korea.
authors: Tae Ryong
authors: TR
authors: Lee
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D001483:Base Sequence; D002460:Cell Line; D015536:Down-Regulation; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C000595033:MIRN526 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1