title: "CLASPing" tungsten's effects on microtubules with "PINs".
abstract: Tungsten, supplied as sodium tungstate, inhibits root elongation in Arabidopsis thaliana, which has been attributed to a diminishing of PIN2 and PIN3 auxin efflux carriers. In this work, we sought to analyze the effect of tungsten on cortical microtubules and CLASP (Cytoplasmic Linker Associated Protein), which are also involved in the anisotropic cell expansion of root cells. Seedlings grown in a tungsten-free substrate for 4 d and then transplanted into a tungsten-containing substrate exhibited randomly oriented microtubules in a time-dependent manner. While tungsten had no effect on roots treated for 3 h, microtubule alignment was obviously affected in the transition and elongation zones after a 6, 12, 24, 48 h tungsten treatment, at prolonged tungsten administrations and in seedlings grown directly in the presence of tungsten. This change in microtubule orientation may be associated with the reduction of CLASP protein expression induced by tungsten, as evidenced in experiments with plants expressing the CLASP-GFP protein. A possible mechanism, by which the coordinated functions of CLASP, PIN2 and microtubules are affected, as revealed by inhibited root growth, is discussed.
authors: a Department of Botany ; School of Biology, Aristotle University ; Thessaloniki , Greece.
authors: Ioannis Dimosthenis S
authors: ID
authors: Adamakis
authors: a Department of Botany ; School of Biology, Aristotle University ; Thessaloniki , Greece.
authors: Emmanuel
authors: E
authors: Panteris
authors: a Department of Botany ; School of Biology, Aristotle University ; Thessaloniki , Greece.
authors: Eleftherios P
authors: EP
authors: Eleftheriou
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007210:Indoleacetic Acids / Q000378:metabolism*; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012989:Soil Pollutants / Q000494:pharmacology*; D014414:Tungsten / Q000494:pharmacology*; D017973:Tungsten Compounds / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D008869:Microtubule-Associated Proteins; D012989:Soil Pollutants; D014414:Tungsten; D017973:Tungsten Compounds; C527272:CLASP protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C449300:PIN3 protein, Arabidopsis; C025399:sodium tungstate(VI)
keywords: CLASP; PIN2; cell expansion; microtubules; tungsten