title: Sustained delivery of chondroitinase ABC by poly(propylene carbonate)-chitosan micron fibers promotes axon regeneration and functional recovery after spinal cord hemisection.
abstract: We describe the sustained delivery of chondroitinase ABC (ChABC) in the hemisected spinal cord using polypropylene carbonate (PPC) electrospun fibers with chitosan (CS) microspheres as a vehicle. PPC and ChABC-loaded CS microspheres were mixed with acetonitrile, and micron fibers were generated by electrospinning. ChABC release was assessed in vitro with high-performance liquid chromatography (HPLC) and revealed stabilized and prolonged release. Moreover, the released ChABC showed sustained activity. PPC-CS micron fibers with or without ChABC were then implanted into a hemisected thoracic spinal cord. In the following 4 weeks, we examined functional recovery and performed immunohistochemical analyses. We found that sustained delivery of ChABC promoted axon sprouting and functional recovery and reduced glial scarring; PPC-CS micron fibers without ChABC did not show these effects. The present findings suggest that PPC-CS micron fibers containing ChABC are a feasible option for spinal cord injury treatment. Furthermore, the system described here may be useful for local delivery of other therapeutic agents.
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, PR China.
authors: Shilei
authors: S
authors: Ni
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, PR China.
authors: Tongliang
authors: T
authors: Xia
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, PR China.
authors: Xingang
authors: X
authors: Li
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, PR China.
authors: Xiaodong
authors: X
authors: Zhu
authors: Institute of Polymer Science & Engineering, Tsinghua University, 30# Shuangqing Road, Beijing 100084, PR China.
authors: Hongxu
authors: H
authors: Qi
authors: Key laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Health and Education, 107# Wenhua Xi Road, Jinan 250012, PR China.
authors: Shanying
authors: S
authors: Huang
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, PR China. Electronic address: drwjg@hotmail.com.
authors: Jiangang
authors: J
authors: Wang
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects*; D048271:Chitosan / Q000627:therapeutic use; D019765:Chondroitin ABC Lyase / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011407:Propane / Q000031:analogs & derivatives / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects*; D012038:Regeneration / Q000187:drug effects*; D013119:Spinal Cord Injuries / Q000188:drug therapy*; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; C045990:propylene carbonate; D048271:Chitosan; D019765:Chondroitin ABC Lyase; D011407:Propane
keywords: ChABC; Chitosan; Electrospinning; PPC; Spinal cord injury