title: Facile synthesis of near infrared fluorescent trypsin-stabilized Ag nanoclusters with tunable emission for 1,4-dihydronicotinamide adenine dinucleotide and ethanol sensing.
abstract: A facile chemical synthetic route was developed to prepare near-infrared fluorescent trypsin-stabilized Ag nanoclusters (Try-Ag NCs). The fluorescence emission wavelength of the produced Try-Ag NCs is tunable by simple adjusting pH value of the synthesis system, and the Try-Ag NCs offer a symmetric fluorescent excitation and emission peak. The fluorescence of Try-Ag NCs remains constant in the presence of various ions and molecules, and it can be effectively quenched by 1,4-dihydronicotinamide adenine dinucleotide (NADH) instead of its oxidized forms nicotinamide adenine dinucleotide (NAD(+)). This property enables the Try-Ag NCs to be a novel analytical platform to monitor biological reaction involved with NADH. In this work, the Try-Ag NCs was also applied to analyze ethanol based on the generation of NADH which was the product of NAD(+) and ethanol in the catalysis of alcohol dehydrogenase. And the proposed platform allowed ethanol to be determined in the range from 10 to 300 μmol/L with 5 μmol/L detection limit.
authors: Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, Shenyang 110000, China.
authors: Siyu
authors: S
authors: Liu
authors: Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, Shenyang 110000, China.
authors: Hui
authors: H
authors: Wang
authors: Molecular Imaging Program at Stanford, Stanford University, Palo Alto, CA 94305, USA.
authors: Zhen
authors: Z
authors: Cheng
authors: Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, Shenyang 110000, China. Electronic address: simonliu@mail.neu.edu.cn.
authors: Hongguang
authors: H
authors: Liu
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques; D000431:Ethanol / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D009243:NAD / Q000032:analysis* / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013552:Swine; D014357:Trypsin / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009243:NAD; D000431:Ethanol; D012834:Silver; D000426:Alcohol Dehydrogenase; D014357:Trypsin
keywords: Ag nanoclusters; Detect; Ethanol; Near-infrared; Trypsin