title: The Antiviral Effect of Baicalin on Enterovirus 71 In Vitro.
abstract: Baicalin is a flavonoid compound extracted from Scutellaria roots that has been reported to possess antibacterial, anti-inflammatory, and antiviral activities. However, the antiviral effect of baicalin on enterovirus 71 (EV71) is still unknown. In this study, we found that baicalin showed inhibitory activity on EV71 infection and was independent of direct virucidal or prophylactic effect and inhibitory viral absorption. The expressions of EV71/3D mRNA and polymerase were significantly blocked by baicalin treatment at early stages of EV71 infection. In addition, baicalin could decrease the expressions of FasL and caspase-3, as well as inhibit the apoptosis of EV71-infected human embryonal rhabdomyosarcoma (RD) cells. Altogether, these results indicate that baicalin exhibits potent antiviral effect on EV71 infection, probably through inhibiting EV71/3D polymerase expression and Fas/FasL signaling pathways.
authors: Department of Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, 62 Huaihai south road, Huai'an, Jiangsu 223300, China. lixiang_suda@163.com.|Department of Clinical Laboratory, the Third Affiliated Hospital of Soochow University, 185 Juqian street, Changzhou, Jiangsu 213003, China. lixiang_suda@163.com.
authors: Xiang
authors: X
authors: Li
authors: Department of Endocrinology, Huai'an First Affliated Hospital of Nanjing Medical University, 6 Beijing west road, Huai'an, Jiangsu 223300, China. liuyy_suda@163.com.
authors: Yuanyuan
authors: Y
authors: Liu
authors: Department of Clinical Laboratory, the Fourth People's Hospital of Huai'an, 128 Yanan east road, Huai'an, Jiangsu 223300, China. wutt8870@163.com.
authors: Tingting
authors: T
authors: Wu
authors: Department of Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, 62 Huaihai south road, Huai'an, Jiangsu 223300, China. huaianjinyue1999@163.com.
authors: Yue
authors: Y
authors: Jin
authors: Department of Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, 62 Huaihai south road, Huai'an, Jiangsu 223300, China. chengjianping2011@aliyun.com.
authors: Jianpin
authors: J
authors: Cheng
authors: Department of Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, 62 Huaihai south road, Huai'an, Jiangsu 223300, China. huaianwancb@163.com.
authors: Changbiao
authors: C
authors: Wan
authors: Department of Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, 62 Huaihai south road, Huai'an, Jiangsu 223300, China. huaianqianwh@163.com.
authors: Weihe
authors: W
authors: Qian
authors: Department of Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College, 62 Huaihai south road, Huai'an, Jiangsu 223300, China. xingfei115@163.com.
authors: Fei
authors: F
authors: Xing
authors: Department of Clinical Laboratory, the Third Affiliated Hospital of Soochow University, 185 Juqian street, Changzhou, Jiangsu 213003, China. shiweifeng67@163.com.
authors: Weifeng
authors: W
authors: Shi
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D029821:Enterovirus A, Human / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000037:antagonists & inhibitors; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D031363:Scutellaria / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D053222:Fas Ligand Protein; D005419:Flavonoids; C038044:baicalin; D012321:DNA-Directed RNA Polymerases
keywords: 3D polymerase; apoptosis; baicalin; enterovirus 71