title: Optimization of Micellar Electrokinetic Chromatography Method for the Simultaneous Determination of Seven Hydrophilic and Four Lipophilic Bioactive Components in Three Salvia Species.
abstract: A micellar electrokinetic chromatography (MEKC) method was developed for the simultaneous determination of seven hydrophilic phenolic acids and four lipophilic tanshinones in three Salvia species. In normal MEKC mode using SDS as surfactant, the investigated 11 compounds could not be well separated. Therefore, several buffer modifiers including β-cyclodextrins (β-CD), ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) and organic solvents have been added to the buffer solution to improve the separation selectivity. Under the optimized conditions (BGE, 15 mM sodium tetraborate with 10 mM SDS, 5 mM β-CD, 10 mM [bmim]BF4 and 15% ACN (v/v) as additives; buffer pH, 9.8; voltage, 20 kV; temperature, 25 °C), the 11 investigated analytes could achieve baseline separation in 34 min. The proposed MEKC was additionally validated by evaluating the linearity (R(2) ≥ 0.9965), LODs (0.27-1.39 μg·mL(-1)), and recovery (94.26%-105.17%), demonstrating this method was reproducible, accurate and reliable. Moreover, the contents of the 11 compounds in three Salvia species, including S. miltiorrhiza, S. przewalskii and S. castanea were analyzed. The result showed that the established MEKC method was simple and practical for the simultaneous determination of the hydrophilic and lipophilic bioactive components in Salvia species, which could be used to effectively evaluate the quality of these valued medicinal plants.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. caojiliang126@126.com.
authors: Jiliang
authors: J
authors: Cao
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. huji_cassie@163.com.
authors: Ji
authors: J
authors: Hu
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. wjc551@sina.com.
authors: Jinchao
authors: J
authors: Wei
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. baocaili@hotmail.com.
authors: Baocai
authors: B
authors: Li
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. kmzhangmi@163.com.
authors: Mi
authors: M
authors: Zhang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. chengxiang0871@126.com.
authors: Cheng
authors: C
authors: Xiang
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. pengli@umac.mo.
authors: Peng
authors: P
authors: Li
mesh_terms: D045784:Abietanes / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D062385:Hydroxybenzoates / Q000737:chemistry*; D027544:Salvia / Q000737:chemistry* / Q000145:classification; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D045784:Abietanes; D062385:Hydroxybenzoates; D012997:Solvents; C021751:tanshinone; D012967:Sodium Dodecyl Sulfate; C017616:phenolic acid
keywords: Salvia species; buffer modifiers; micellar electrokinetic chromatography; phenolic acids; tanshinones