title: Combination of counterpropagation artificial neural networks and antioxidant activities for comprehensive evaluation of associated-extraction efficiency of various cyclodextrins in the traditional Chinese formula Xue-Zhi-Ning.
abstract: Xue-Zhi-Ning (XZN) is a widely used traditional Chinese medicine formula to treat hyperlipidemia. Recently, cyclodextrins (CDs) have been extensively used to minimize problems relative to medicine bioavailability, such as low solubility and poor stability. The objective of this study was to determine the associated-extraction efficiency of various CDs in XZN. Three various type CDs were evaluated, including native CDs (α-CD, β-CD), hydrophilic CD derivatives (HP-β-CD and Me-β-CD), and ionic CD derivatives (SBE-β-CD and CM-β-CD). An ultra high-performance liquid chromatography (UHPLC) fingerprint was applied to determine the components in CD extracts and original aqueous extract (OAE). A counterpropagation artificial neural network (CP-ANN) was used to analyze the components in different extracts and compare the selective extraction of various CDs. Extraction efficiencies of the various CDs in terms of extracted components follow the ranking, ionic CD derivatives>hydrophilic CD derivatives>native CDs>OAE. Besides, different types of CDs have their own selective extraction and ionic CD derivatives present the strongest associated-extraction efficiency. Antioxidant potentials of various extracts were evaluated by determining the inhibition of spontaneous, H2O2-induced, CCl4-induced and Fe(2+)/ascorbic acid-induced lipid peroxidation (LPO) and analyzing the scavenging capacity for DPPH and hydroxyl radicals. The order of extraction efficiencies of the various CDs relative to antioxidant activities is as follows: SBE-β-CD>CM-β-CD>HP-β-CD>Me-β-CD>β-CD>α-CD. It can be demonstrated that all of the CDs studied increase the extraction efficiency and that ionic CD derivatives (SBE-β-CD and CM-β-CD) present the highest extraction capability in terms of amount extracted and antioxidant activities of extracts.
authors: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors: Lili
authors: L
authors: Sun
authors: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors: Jianwen
authors: J
authors: Yang
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors: Meng
authors: M
authors: Wang
authors: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors: Huijie
authors: H
authors: Zhang
authors: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors: Yanan
authors: Y
authors: Liu
authors: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. Electronic address: renxiaoliang@tjutcm.edu.cn.
authors: Xiaoliang
authors: X
authors: Ren
authors: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors: Aidi
authors: A
authors: Qi
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation; D016571:Neural Networks, Computer; D010851:Picrates / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D003505:Cyclodextrins; D004365:Drugs, Chinese Herbal; D010851:Picrates; C000608784:xue-zhi-ning; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activities; Associated-extraction efficiency; Counterpropagation artificial neural network (CP-ANN); Cyclodextrins; Kohonen maps; Traditional Chinese formula Xue-Zhi-Ning (XZN)