title: Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System.
abstract: The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This portable biosensing system designed for field use is characterized by a special structure which consists of a microfluidic cell incorporating a right angle prism functionalized with a biomolecular identification membrane, a laser line generator and a data acquisition circuit board. The data structure, data memory capacity and a line charge-coupled device (CCD) array with a driving circuit for collecting the photoelectric signals are intensively focused on and the high performance analog-to-digital (A/D) converter is comprehensively evaluated. The interface circuit and the photoelectric signal amplifier circuit are first studied to obtain the weak signals from the line CCD array in this experiment. Quantitative measurements for validating the sensitivity of the biosensing system were implemented using ethanol solutions of various concentrations indicated by volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a biomembrane immobilized on the surface of the SPR sensor. The experiments demonstrated that it is possible to detect a change in the refractive index of an ethanol solution with a sensitivity of 4.99838 × 10(5) ΔRU/RI in terms of the changes in delta response unit with refractive index using this SPR biosensing system, whereby the theoretical limit of detection of 3.3537 × 10(-5) refractive index unit (RIU) and a high linearity at the correlation coefficient of 0.98065. The results obtained from a series of tests confirmed the practicality of this cost-effective portable SPR biosensing system.
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. changkeke927@163.com.
authors: Keke
authors: K
authors: Chang
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. chen_ruipeng@yeah.net.
authors: Ruipeng
authors: R
authors: Chen
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. wangshun6518@163.com.
authors: Shun
authors: S
authors: Wang
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. hauljw@163.com.
authors: Jianwei
authors: J
authors: Li
authors: School of Human Nutrition and Dietetics, McGill University, Ste Anne de Bellevue, QC H9X 3V9, Canada. xinran.hu@mail.mcgill.ca.
authors: Xinran
authors: X
authors: Hu
authors: Department of Electronic and Telecommunications, University of Gävle, Gävle SE-801 76, Sweden. atn23cn@163.com.
authors: Hao
authors: H
authors: Liang
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. caobaiqiong@163.com.
authors: Baiqiong
authors: B
authors: Cao
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. xiaohuosun89@163.com.
authors: Xiaohui
authors: X
authors: Sun
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. mlz0124@126.com.
authors: Liuzheng
authors: L
authors: Ma
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. zhujh88@163.com.
authors: Juanhua
authors: J
authors: Zhu
authors: College of life sciences, Henan Agricultural University, Zhengzhou 450002, China. jm_hu@163.com.
authors: Min
authors: M
authors: Jiang
authors: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China. jiandonghu@163.com.|State key laboratory of wheat and maize crop science, Zhengzhou 450002, China. jiandonghu@163.com.
authors: Jiandong
authors: J
authors: Hu
mesh_terms: D000703:Analog-Digital Conversion; D002138:Calibration; D004581:Electronics; D004867:Equipment Design; D000431:Ethanol / Q000032:analysis; D012815:Signal Processing, Computer-Assisted; D012996:Solutions; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D012996:Solutions; D000431:Ethanol
keywords: ADC conversion time; biosensing system; linear CCD array; microcontroller; refractive index; surface plasmon resonance