title: Auxiliary diagnostic value of monocyte chemoattractant protein-1 of whole blood in active tuberculosis.
abstract: The aim of this study was to study the expression level of interferon-γ (IFN-γ) and monocyte chemoattractant protein-1 (MCP-1) in peripheral blood and its auxiliary diagnostic value in active tuberculosis. A chemiluminescence enzyme immunoassay method was used to detect the levels of IFN-γ and MCP-1 in peripheral blood. Then the receiver operating characteristic curve were drawn to determine the threshold of IFN-γ and MCP-1 for diagnosis of active tuberculosis and to evaluate their diagnostic performance. The specific IFN-γ and MCP-1 levels in the active tuberculosis group were significantly higher than those in the non-tuberculous pulmonary disease group (P < 0.01) and those in the healthy control group (P < 0.01). The IFN-γ levels in the healthy control group and the non-tuberculous respiratory disease group showed no statistically significant difference (P > 0.05), but the MCP-1 levels in the non-tuberculous respiratory disease group were significantly higher than those of the healthy control group (P < 0.05). The specific IFN-γ and MCP-1 level cut off values were 256 pg/ml and 389 pg/ml as an active tuberculosis diagnostic standard. The sensitivities of IFN-γ and MCP-1 were 57.3% and 92.8%, respectively; specificities were 80% and 80.7%, respectively; the positive predictive values were 76.9% and 84.9%, respectively; negative predictive values were 61.7% and 78.7%, respectively; and accuracy rates were 76.9% and 84.9%, respectively. Compared with the detection of IFN-γ, we observed a better diagnostic performance of MCP-1 in peripheral blood in active tuberculosis. MCP-1 may become one of the active tuberculosis auxiliary diagnostic targets.
authors: Department of Laboratory, The Second Hospital of Jilin University Changchun 130041, China.
authors: Ying
authors: Y
authors: Wang
authors: Department of Thoracic Surgery, The Tumor Hospital of Jilin Province Changchun 130021, China.
authors: Hang
authors: H
authors: Li
authors: Department of Laboratory, The Second Hospital of Jilin University Changchun 130041, China.
authors: Hong
authors: H
authors: Bao
authors: Department of Laboratory, The Second Hospital of Jilin University Changchun 130041, China.
authors: Yufen
authors: Y
authors: Jin
authors: Department of Laboratory, The Second Hospital of Jilin University Changchun 130041, China.
authors: Xiaoju
authors: X
authors: Liu
authors: Department of Tuberculosis Research Laboratory, The 309th Hospital of PLA Beijing 100091, China.
authors: Xueqiong
authors: X
authors: Wu
authors: Department of Laboratory, The Second Hospital of Jilin University Changchun 130041, China.
authors: Ting
authors: T
authors: Yu
keywords: IFN-γ; Tuberculosis; diagnosis; monocyte chemoattractant protein-1