title: Comparing the Hem- and Lymphangiogenic Profile of Conjunctival and Uveal Melanoma Cell Lines.
abstract: OBJECTIVE
  Malignant melanomas of the ocular surface (conjunctival melanoma [CM]) and within the eye (uveal melanoma [UM]) show different types of metastatic behavior. While CM has a propensity to spread first to regional lymph nodes, UM metastasizes almost exclusively via the hematogenic route to the liver. We investigated whether these different metastatic patterns might be attributable to differential hem- and lymphangiogenic characteristics of CM and UM cells.
  
  
  METHODS
  Human CM (CM2005.1, CRMM1, CRMM2) and UM (Mel270, Mel290, OM431) cell lines were analyzed for VEGF-A, -C, and -D expression by RT-PCR and ELISA. The influence of CM- or UM-conditioned medium on blood (BEC) and lymphatic (LEC) endothelial cell proliferation and migration was measured using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide (MTT) and scratch assays, respectively.
  
  
  RESULTS
  Vascular endothelial growth factor-A, -C and -D mRNA, and VEGF-A and -D protein were expressed by all CM and UM cell lines, while VEGF-C protein was only expressed by UM cell lines. The CM- and UM-conditioned medium did neither differentially affect BEC (P = 0.86) and LEC (P = 0.90) proliferation, nor BEC (P = 0.56) and LEC (P = 0.90) migration.
  
  
  CONCLUSIONS
  Conjunctival melanoma cell lines did not show a higher prolymphangiogenic potential, and UM cell lines did not show a higher prohemangiogenic potential. Accordingly, other mechanisms within the tumor microenvironment might account for the diverging metastatic patterns of conjunctival versus uveal melanomas.
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Nasrin
authors: N
authors: Refaian
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Simona L
authors: SL
authors: Schlereth
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Konrad R
authors: KR
authors: Koch
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Maria
authors: M
authors: Notara
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Deniz
authors: D
authors: Hos
authors: Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany.
authors: Melina
authors: M
authors: Mescher
authors: Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany.
authors: Sandra
authors: S
authors: Iden
authors: Department of Internal Medicine 5, Hematology and Oncology, University of Erlangen-Nuremberg, Erlangen, Germany.
authors: Jacobus J
authors: JJ
authors: Bosch
authors: Department of Ophthalmology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
authors: Martine J
authors: MJ
authors: Jager
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Claus
authors: C
authors: Cursiefen
authors: Department of Ophthalmology University of Cologne, Cologne, Germany.
authors: Ludwig M
authors: LM
authors: Heindl
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003230:Conjunctival Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D042601:Lymphatic Vessels / Q000378:metabolism / Q000473:pathology*; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics*; D014604:Uveal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics*; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis / Q000235:genetics*; D000098943:Uveal Melanoma
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042582:Vascular Endothelial Growth Factor C