title: NAD(+)-aminoaldehyde dehydrogenase candidates for 4-aminobutyrate (GABA) and β-alanine production during terminal oxidation of polyamines in apple fruit.
abstract: The last step of polyamine catabolism involves the oxidation of 3-aminopropanal or 4-aminobutanal via aminoaldehyde dehydrogenase. In this study, two apple (Malus x domestica) AMADH genes were selected (MdAMADH1 and MdAMADH2) as candidates for encoding 4-aminobutanal dehydrogenase activity. Maximal activity and catalytic efficiency were obtained with NAD(+) and 3-aminopropanal, followed by 4-aminobutanal, at pH 9.8. NAD(+) reduction was accompanied by the production of GABA and β-alanine, respectively, when 4-aminobutanal and 3-aminopropanal were utilized as substrates. MdAMADH2 was peroxisomal and MdAMADH1 cytosolic. These findings shed light on the potential role of apple AMADHs in 4-aminobutyrate and β-alanine production.
authors: Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: azarei@uoguelph.ca.
authors: Adel
authors: A
authors: Zarei
authors: Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: christophert@nutriag.com.
authors: Christopher P
authors: CP
authors: Trobacher
authors: Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: bshelp@uoguelph.ca.
authors: Barry J
authors: BJ
authors: Shelp
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism; D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003600:Cytosol / Q000378:metabolism; D005638:Fruit / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D027845:Malus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D020675:Peroxisomes / Q000378:metabolism; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism*; D011437:Propylamines / Q000378:metabolism; D021381:Protein Transport; D011523:Protoplasts / Q000378:metabolism; D013379:Substrate Specificity; D015091:beta-Alanine / Q000096:biosynthesis*; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
chemical_list: D000447:Aldehydes; D011073:Polyamines; D011437:Propylamines; D009243:NAD; D015091:beta-Alanine; D005680:gamma-Aminobutyric Acid; D000445:Aldehyde Oxidoreductases; C050862:3-aminopropionaldehyde
keywords: 4-Aminobutanal; 4-Aminobutyrate; Aminoaldehyde dehydrogenase; Apple; Peroxisomal targeting signal; Polyamine