title: Diversity of Lactobacillus reuteri Strains in Converting Glycerol into 3-Hydroxypropionic Acid.
abstract: The present study aims at comparing the performances of three Lactobacillus reuteri strains (DSM 20016, DSM 17938, and ATCC 53608) in producing 3-hydroxypropionic acid (3-HP) from glycerol and at exploring inhibition phenomena during this bioconversion. Differences were highlighted between the three strains in terms of 3-HP production yield, kinetics of substrate consumption, and metabolite production. With a maximal productivity in non-optimal conditions (free pH) around 2 g.L(-1).h(-1) of 3-HP and 4 g.L(-1).h(-1) of 3-hydroxypropionaldehyde (3-HPA) depending on the strain, this study confirmed the potential of L. reuteri for the biotechnological production of 3-HP. Moreover, the molar ratios of 3-HP to 1,3-propanediol (1,3-PDO) obtained for the three strains (comprised between 1.25 and 1.65) showed systematically a higher 3-HP production. From these results, the DSM 17938 strain appeared to be the most promising strain. The impact of glycerol bioconversion on the bacteria's physiological state (a decrease of around 40 % in DSM 17938 cells showing an enzymatic activity after 3 h) and survival (total loss of cultivability after 2 or 3 h depending on the strains) was revealed and discussed. The effect of each metabolite on L. reuteri DSM 17938 was further investigated, displaying a drastic inhibition caused by 3-HPA, while 3-HP induced lower impact and only at acidic pH.
authors: Chaire Agro-Biotechnologies Industrielles (ABI)-AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100, Reims, France.|AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors: G
authors: G
authors: Burgé
authors: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors: C
authors: C
authors: Saulou-Bérion
authors: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors: M
authors: M
authors: Moussa
authors: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors: B
authors: B
authors: Pollet
authors: Chaire Agro-Biotechnologies Industrielles (ABI)-AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100, Reims, France.|AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Route de Saint-Cyr, F-78026, Versailles Cedex, France.|INRA, Institut Jean-Pierre Bourgin (IJPB), Route de Saint-Cyr, F-78026, Versailles Cedex, France.
authors: A
authors: A
authors: Flourat
authors: Chaire Agro-Biotechnologies Industrielles (ABI)-AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100, Reims, France.|AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors: F
authors: F
authors: Allais
authors: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors: V
authors: V
authors: Athès
authors: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France. spinnler@agroparistech.fr.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France. spinnler@agroparistech.fr.
authors: H E
authors: HE
authors: Spinnler
mesh_terms: D044822:Biodiversity; D001711:Biotransformation; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D011422:Propionates; D019344:Lactic Acid; C031601:hydracrylic acid; C029658:propionic acid; D005990:Glycerol
keywords: 3-Hydroxypropionic acid; Glycerol bioconversion; Inhibitory activity; Lactobacillus reuteri