title: Centrosomin represses dendrite branching by orienting microtubule nucleation.
abstract: Neuronal dendrite branching is fundamental for building nervous systems. Branch formation is genetically encoded by transcriptional programs to create dendrite arbor morphological diversity for complex neuronal functions. In Drosophila sensory neurons, the transcription factor Abrupt represses branching via an unknown effector pathway. Targeted screening for branching-control effectors identified Centrosomin, the primary centrosome-associated protein for mitotic spindle maturation. Centrosomin repressed dendrite branch formation and was used by Abrupt to simplify arbor branching. Live imaging revealed that Centrosomin localized to the Golgi cis face and that it recruited microtubule nucleation to Golgi outposts for net retrograde microtubule polymerization away from nascent dendrite branches. Removal of Centrosomin enabled the engagement of wee Augmin activity to promote anterograde microtubule growth into the nascent branches, leading to increased branching. The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.|Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan.
authors: Cagri
authors: C
authors: Yalgin
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Saman
authors: S
authors: Ebrahimi
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Caroline
authors: C
authors: Delandre
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Li Foong
authors: LF
authors: Yoong
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Saori
authors: S
authors: Akimoto
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Heidi
authors: H
authors: Tran
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Reiko
authors: R
authors: Amikura
authors: Institute for Genomics and Systems Biology, Department of Human Genetics, The University of Chicago, Chicago, Illinois, USA.
authors: Rebecca
authors: R
authors: Spokony
authors: Computational Neuroscience Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Kunigami, Okinawa, Japan.
authors: Benjamin
authors: B
authors: Torben-Nielsen
authors: Institute for Genomics and Systems Biology, Department of Human Genetics, The University of Chicago, Chicago, Illinois, USA.
authors: Kevin P
authors: KP
authors: White
authors: Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Adrian W
authors: http://orcid.org/0000-0002-8242-301X
authors: AW
authors: Moore
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D016764:Cell Polarity; D047369:Chromatin Immunoprecipitation; D003712:Dendrites / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011984:Sensory Receptor Cells / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C098130:cnn protein, Drosophila