title: Isolation and molecular characterization of Toxoplasma gondii in a colony of captive black-capped squirrel monkeys (Saimiri boliviensis).
abstract: Toxoplasmosis is commonly asymptomatic; however, it can be a fatal multisystemic disease in some animal species, such as New World monkeys. An outbreak of acute fatal toxoplasmosis was reported in a colony of black-capped squirrel monkeys (Saimiri boliviensis) from the zoo of La Plata, Argentina. Post-mortem examination of two monkeys revealed macroscopical and microscopical lesions compatible with acute toxoplasmosis. The presence of Toxoplasma gondii was confirmed by immunohistochemistry on monkey tissues, bioassay in mice and PCR using the specific primers B22-B23. By PCR-RFLP analysis, T. gondii isolated in mice, deriving from both monkeys, showed the same restriction pattern, with most markers showing a type III restriction pattern, except for C22-8 (type II) and C29-2 (type I). To our knowledge this is the first report of fatal toxoplasmosis in S. boliviensis caused by a non-canonical or atypical genotype of T. gondii.
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CABA, Buenos Aires, Argentina. Electronic address: laispardini@fcv.unlp.edu.ar.
authors: L
authors: L
authors: Pardini
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CABA, Buenos Aires, Argentina.
authors: A
authors: A
authors: Dellarupe
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina.
authors: D
authors: D
authors: Bacigalupe
authors: Laboratorio de Patología Especial Veterinaria, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina.
authors: M A
authors: MA
authors: Quiroga
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CABA, Buenos Aires, Argentina.
authors: G
authors: G
authors: Moré
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina.
authors: M
authors: M
authors: Rambeaud
authors: Institute of Parasitology, Vetsuisse-Faculty, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland.
authors: W
authors: W
authors: Basso
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina.
authors: J M
authors: JM
authors: Unzaga
authors: Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Epidemiology, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
authors: G
authors: G
authors: Schares
authors: Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, La Plata 1900, Buenos Aires, Argentina.
authors: M C
authors: MC
authors: Venturini
mesh_terms: D000818:Animals; D000836:Animals, Zoo / Q000469:parasitology*; D001118:Argentina; D016054:DNA, Protozoan / Q000235:genetics; D004196:Disease Outbreaks; D005838:Genotype; D008297:Male; D051379:Mice; D008992:Monkey Diseases / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012453:Saimiri / Q000469:parasitology*; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan
keywords: Genotyping; Histopathology; Isolation; PCR; Saimiri boliviensis; Toxoplasma gondii