title: Rhodium Complex and Enzyme Couple Mediated Electrochemical Detection of Adenosine.
abstract: Adenosine is one of the nucleoside which plays an important role in signal transduction and neuromodulation. This work proposes a simple electrochemical assay, comprising two enzymes and rhodium complex based electron transfer mediator, for the detection of adenosine. Sequential reaction of adenosine deaminase and L-glutamic dehydrogenase and the supporting cycle between β-NADH and mediator enable quantitative analysis of adenosine. Role of electron transfer mediator is the conveyance of proton from electrode to β-NAD(+) for regeneration of β-NADH. The electrochemical characteristics of electron transfer mediator were also studied. Real-time adenosine detection was carried out using this multiple enzyme based chronoamperometric assay. The analysis results show a low limit of detection (140 μM) and good correspondence between current signal and the adenosine concentration (R (2) = 0.997).
authors: School of Electronic and Electrical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors: Dawoon
authors: D
authors: Han
authors: Department of Chemistry, Seoul National University, Seoul, 151-747, Republic of Korea.
authors: Hyeong-Mook
authors: HM
authors: Kim
authors: School of Electronic and Electrical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors: Rohit
authors: R
authors: Chand
authors: School of Electronic and Electrical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors: Gyumin
authors: G
authors: Kim
authors: Department of Chemistry, Soongsil University, Seoul, 156-743, Republic of Korea. extant@ssu.ac.kr.
authors: Ik-Soo
authors: IS
authors: Shin
authors: School of Electronic and Electrical Engineering, Sungkyunkwan University, Suwon, Republic of Korea. yongsang@skku.edu.
authors: Yong-Sang
authors: YS
authors: Kim
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012238:Rhodium / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D009942:Organometallic Compounds; D009243:NAD; D012238:Rhodium; D000241:Adenosine
keywords: Adenosine; Electrochemical; Electron transfer mediator; Enzymatic reaction; Nafion