title: Preparation and Characterization of Fully Active Biotinylated Analogs of Phytosulfokine-α.
abstract: We report the preparation of biotinylated analogs of phytosulfokine-α (Tyr(SO3H)-Ile-Tyr(SO3H)-Thr-Gln; PSK-α), an endogenous peptide growth factor in plants. Because the modification of the N-terminal amino group leads to significant loss of the activities, a Lys residue was incorporated in the C-terminal region of PSK-α, and its e amino group was reacted with biotinylation reagent. Results of the binding assay showed that [N(ε)-(biotinyl)Lys(5)]PSK-α retained the same binding activity and mitogenic activity as that of native PSK-α. Insertion of a single or double 6-aminohexanoic acid spacer between the ε amino group of Lys(5) and the carboxyl group of biotin did not significantly alter the activities of biotinylated [Lys(5)]PSK-α. Structure-activity information obtained here would be useful for the detection and isolation of PSK-α receptors.
authors: a Laboratory of Bioactive Natural Products Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya University.
authors: Y
authors: Y
authors: Matsubayashi
authors: Y
authors: Y
authors: Takahata
authors: A
authors: A
authors: Morita
authors: K
authors: K
authors: Atsumi
authors: Y
authors: Y
authors: Sakagami
mesh_terms: D027761:Asparagus Plant / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D004305:Dose-Response Relationship, Drug; D008861:Microsomes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D012275:Oryza / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D036361:Peptide Hormones / Q000138:chemical synthesis* / Q000494:pharmacology; D059828:Plant Cells / Q000737:chemistry / Q000187:drug effects; D010937:Plant Growth Regulators / Q000138:chemical synthesis* / Q000494:pharmacology; D010940:Plant Proteins / Q000819:agonists / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000819:agonists / Q000737:chemistry / Q000378:metabolism
chemical_list: C100975:PSK-alpha protein, plant; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C409473:phytosulfokine-alpha receptor, plant; D001710:Biotin
keywords: binding assay; biotin; growth factor; phytosulfokine-α; receptor