title: Lipopolysaccharide induction of REDD1 is mediated by two distinct CREB-dependent mechanisms in macrophages.
abstract: REDD1 is induced by various cellular stresses; however, its expression in response to lipopolysaccharide (LPS) has not been clearly elucidated in immune cells. LPS stimulated CREB-dependent and NF-κB-independent REDD1 expression in macrophages. Early increases in CREB phosphorylation and REDD1 expression at 8h following LPS treatment were blocked by inhibition of p38MAPK and mitogen- and stress-activated protein kinase 1 (MSK1), but not PKA. However, delayed CREB-mediated REDD1 expression at 16h was suppressed by inhibition of cyclooxygenase-2 (COX-2) and PKA. It indicates that LPS induces REDD1 expression by two distinct CREB-mediated mechanisms, the early p38MAPK/MSK1 and the delayed COX-2/PGE2/PKA pathways.
authors: Departments of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Dong-Keon
authors: DK
authors: Lee
authors: Departments of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Ji-Hee
authors: JH
authors: Kim
authors: Departments of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Wan-Sung
authors: WS
authors: Kim
authors: Departments of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Dooil
authors: D
authors: Jeoung
authors: Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Hansoo
authors: H
authors: Lee
authors: Departments of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Kwon-Soo
authors: KS
authors: Ha
authors: Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea.
authors: Moo-Ho
authors: MH
authors: Won
authors: Department of Biochemistry, College of Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea.
authors: Young-Guen
authors: YG
authors: Kwon
authors: Departments of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 200-701, South Korea. Electronic address: ymkim@kangwon.ac.kr.
authors: Young-Myeong
authors: YM
authors: Kim
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; C469992:Ddit4 protein, mouse; D008070:Lipopolysaccharides; D014157:Transcription Factors; D003994:Bucladesine; D051546:Cyclooxygenase 2; D038744:Ribosomal Protein S6 Kinases, 90-kDa; C116919:mitogen and stress-activated protein kinase 1; D017868:Cyclic AMP-Dependent Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D015232:Dinoprostone
keywords: CREB activation; Cyclooxygenase-2; Mitogen- and stress-activated protein kinase 1; Protein kinase A; Regulated in development and DNA damage response 1