title: EGCG induces G-CSF expression and neutrophilia in experimental sepsis.
abstract: A major green tea component, epigallocatechin-3-gallate (EGCG), has been proven protective against lethal sepsis in experimental setting, but its protective mechanisms remain incompletely understood. Here, we provide evidence to support EGCG's capacities in stimulating G-CSF production and neutrophilia in vivo. In an animal model of sepsis, EGCG significantly elevated peritoneal levels of G-CSF and several chemokines (e.g., MCP-1/CCL2 and MIP-1γ/CCL9), and consequently increased peritoneal neutrophil numbers (neutrophilia) at a late stage. In vitro, EGCG divergently affected HMGB1-mediated production of several chemokines: reducing CXCL15 and RANTES/CCL5, but elevating G-CSF and MIP-1α/CCL3 production by peritoneal macrophages. Similarly, it significantly induced the expression and secretion of G-CSF and MIP-1α/CCL3 in human peripheral blood mononuclear cells. Based on our preliminary data, it may be important to search for anti-inflammatory and G-CSF-stimulating agents for the clinical management of inflammatory diseases.
authors: Department of Emergency Medicine, North Shore University Hospital, 350 Community Drive, Manhasset, NY, 11030, USA.|The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, 11030, USA.
authors: Wei
authors: W
authors: Li
authors: Department of Emergency Medicine, North Shore University Hospital, 350 Community Drive, Manhasset, NY, 11030, USA.|The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, 11030, USA.
authors: Andrew H
authors: AH
authors: Wu
authors: Department of Emergency Medicine, North Shore University Hospital, 350 Community Drive, Manhasset, NY, 11030, USA.|The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, 11030, USA.
authors: Shu
authors: S
authors: Zhu
authors: The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, 11030, USA.
authors: Jianhua
authors: J
authors: Li
authors: The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, 11030, USA.
authors: Rong
authors: R
authors: Wu
authors: Department of Emergency Medicine, North Shore University Hospital, 350 Community Drive, Manhasset, NY, 11030, USA.
authors: John
authors: J
authors: D'Angelo
authors: Department of Emergency Medicine, North Shore University Hospital, 350 Community Drive, Manhasset, NY, 11030, USA. hwang@nshs.edu.|The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, 11030, USA. hwang@nshs.edu.
authors: Haichao
authors: H
authors: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D024243:HMGB1 Protein / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D018805:Sepsis / Q000276:immunology* / Q000628:therapy; D013662:Tea / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D018925:Chemokines; D024243:HMGB1 Protein; D013662:Tea; D016179:Granulocyte Colony-Stimulating Factor; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Cytokine antibody array; Green tea; Innate immune cells; Sepsis