title: Investigations into the binding of jadomycin DS to human topoisomerase IIβ by WaterLOGSY NMR spectroscopy.
abstract: The jadomycins are a family of secondary metabolites produced by S. venezuelae ISP5230. Specific jadomycins have been shown to possess a variety of anticancer, antifungal, and antibacterial properties, with different molecular mechanisms of action. Herein we demonstrate qualitative and quantitative direct binding between the validated anticancer target human topoisomerase IIβ and jadomycin DS using WaterLOGSY NMR spectroscopy. Additionally, we report for the first time, that jadomycin DS also binds a variety of other proteins, likely in a non-specific manner. Such interactions may rationalize the potential polypharmacology of jadomycin DS.
authors: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
authors: Camilo F
authors: CF
authors: Martinez-Farina
authors: Nicole
authors: N
authors: McCormick
authors: Andrew W
authors: AW
authors: Robertson
authors: Helen
authors: H
authors: Clement
authors: Alison
authors: A
authors: Jee
authors: Anna
authors: A
authors: Ampaw
authors: Nei-Li
authors: NL
authors: Chan
authors: Ray T
authors: RT
authors: Syvitski
authors: David L
authors: DL
authors: Jakeman
mesh_terms: D001665:Binding Sites; D004250:DNA Topoisomerases, Type II / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007546:Isoquinolines / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; D007546:Isoquinolines; C000609367:jadomycin DS; D014867:Water; D004250:DNA Topoisomerases, Type II