title: Growth Inhibition Accompanied by MOB1 Upregulation in Human Acute Lymphoid Leukemia Cells by 3-Deazaneplanocin A.
abstract: Our purpose was to investigate the effect of 3-deazaneplanocin A (DZNep) on human T-cell acute lymphoid leukemia (T-ALL) cells, and to explore the underlying molecular mechanisms. The human T-ALL cell line Molt4 was treated with DZNep, and cell proliferation was examined. The expression of Mps one binder kinase activator 1 gene (MOB1) mRNA and protein was determined by RT-PCR and Western blotting, respectively. The histone modification effect of DZNep on the lysine 9 of histone 3 associated with MOB1 promoters was examined with chromatin immunoprecipitation and quantitative PCR, and CpG methylation in MOB1 promoters was detected by bisulfite sequencing PCR. DZNep treatment inhibited the growth of Molt4 cells. The expressions of MOB1 genes were upregulated by DZNep treatment, and histone methylations in their promoters were significantly reduced. The results indicate that DZNep is a promising therapeutic compound for the treatment of human T-ALL.
authors: Department of Hematology, Fujian Institute of Hematology, Union Hospital of Fujian Medical University, Fuzhou, 35001, Fujian, People's Republic of China, suunchina@163.com.
authors: Jianzhen
authors: J
authors: Shen
authors: Junnan
authors: J
authors: Su
authors: Dansen
authors: D
authors: Wu
authors: Feng
authors: F
authors: Zhang
authors: Haiying
authors: H
authors: Fu
authors: Huarong
authors: H
authors: Zhou
authors: Meihong
authors: M
authors: Xu
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D054357:Chemokine CXCL10 / Q000378:metabolism*; D018899:CpG Islands; D006657:Histones / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism* / Q000473:pathology; D011401:Promoter Regions, Genetic; D015854:Up-Regulation
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D006657:Histones; C048460:3-deazaneplanocin; D008239:Lysine; D000241:Adenosine