title: Protective immunity of rAd5/NR2B vaccine against concomitant aversiveness of spontaneous neuropathic pain following spinal nerve ligation injury.
abstract: OBJECTIVE
  Peripheral nerve injury elicits an aversive state of spontaneous neuropathic pain, and up to now, the modulation of this concomitant aversive state remains a major therapeutic challenge. NMDA receptor subunits NR2B in the rACC are critically involved in the processing of this aversive state and then a strategy targeted at the NR2B subunit might be promising for modulation of the aversive state. Thus, in the present study, using negative reinforcement animal model to reveal spontaneous pain, we investigated the effect of oral immunization with recombinant adenovirus serotype 5-mediated NR2B gene transfer (rAd5/NR2B) on the modulation of the tonic pain.
  
  
  METHODS
  Following oral administration of the rAd5/NR2B vaccine, NR2B-specific antibodies were induced in serum. And the humoral response was involved in the decreased expression of NR2B protein in the rACC.
  
  
  RESULTS
  The present study demonstrated that CPP achieved by spinal administration of clonidine in spinal nerve ligation (SNL) rats revealed the presence of aversive state of spontaneous neuropathic pain. Notably, the humoral autoimmune response blocked the CPP by spinal clonidine, suggesting the relief of the concomitant aversive of spontaneous neuropathic pain in the SNL rats.
  
  
  CONCLUSIONS
  These data proved the feasibility of oral immunization with rAd5/NR2B for modulation of concomitant aversive of spontaneous neuropathic pain due to peripheral nerve injury.
authors: Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, China.
authors: Gong-Ming
authors: GM
authors: Wang
authors: Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, China.
authors: Xiao-Yan
authors: XY
authors: Wang
authors: Department of Anesthesiology, Jinan Sixth People's Hospital Jinan 250021, China.
authors: Guang-Jie
authors: GJ
authors: Liu
authors: Department of Anesthesiology, Zouping Hospital Affiliated to Taishan Medical University Zouping 256200, China.
authors: Kun
authors: K
authors: Cheng
authors: Department of Pharmacy, Taian Central Hospital Taian 271000, China.
authors: Hua
authors: H
authors: Wang
authors: Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, China.
authors: Shou-Gang
authors: SG
authors: Guo
keywords: NR2B; Spontaneous neuropathic pain; anterior cingulate cortex; immunity