title: Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome.
abstract: Adeno-associated virus type 2 is known to inhibit replication of herpes simplex virus 1 (HSV-1). This activity has been linked to the helicase- and DNA-binding domains of the Rep68/Rep78 proteins. Here, we show that Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome and that the Rep helicase activity can inhibit replication of any DNA if binding is facilitated. Therefore, we hypothesize that inhibition of HSV-1 replication involves direct binding of Rep68/Rep78 to the HSV-1 genome.
authors: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors: Michael
authors: M
authors: Seyffert
authors: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors: Daniel L
authors: DL
authors: Glauser
authors: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors: Kurt
authors: K
authors: Tobler
authors: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
authors: Oleg
authors: O
authors: Georgiev
authors: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors: Rebecca
authors: R
authors: Vogel
authors: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors: Bernd
authors: B
authors: Vogt
authors: Department of Infectious Diseases, King's College London, London, United Kingdom.
authors: Leticia
authors: L
authors: Agúndez
authors: Department of Infectious Diseases, King's College London, London, United Kingdom.
authors: Michael
authors: M
authors: Linden
authors: Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany Institute for Experimental Hematology, Hannover Medical School, Hannover, Germany.
authors: Hildegard
authors: H
authors: Büning
authors: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors: Mathias
authors: M
authors: Ackermann
authors: Institute of Virology, University of Zurich, Zurich, Switzerland cornel.fraefel@access.uzh.ch.
authors: Cornel
authors: C
authors: Fraefel
mesh_terms: D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D004265:DNA Helicases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D016679:Genome, Viral / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2; D004265:DNA Helicases