title: Methylocapsa palsarum sp. nov., a methanotroph isolated from a subArctic discontinuous permafrost ecosystem.
abstract: An aerobic methanotrophic bacterium was isolated from a collapsed palsa soil in northern Norway and designated strain NE2T. Cells of this strain were Gram-stain-negative, non-motile, non-pigmented, slightly curved thick rods that multiplied by normal cell division. The cells possessed a particulate methane monooxygenase enzyme (pMMO) and utilized methane and methanol. Strain NE2T grew in a wide pH range of 4.1–8.0 (optimum pH 5.2–6.5) at temperatures between 6 and 32 °C (optimum 18–25 °C), and was capable of atmospheric nitrogen fixation under reduced oxygen tension. The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and C16 : 1ω7c, and the DNA G+C content was 61.7 mol%. The isolate belonged to the family Beijerinckiaceae of the class Alphaproteobacteria and was most closely related to the facultative methanotroph Methylocapsa aurea KYGT (98.3 % 16S rRNA gene sequence similarity and 84 % PmoA sequence identity). However, strain NE2T differed from Methylocapsa aurea KYGT by cell morphology, the absence of pigmentation, inability to grow on acetate, broader pH growth range, and higher tolerance to NaCl. Therefore, strain NE2T represents a novel species of the genus Methylocapsa, for which we propose the name Methylocapsa palsarum sp. nov. The type strain is NE2T ( = LMG 28715T = VKM B-2945T).
authors: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors: Svetlana N
authors: SN
authors: Dedysh
authors: UiT The Arctic University of Norway, Department of Arctic and Marine Biology, 9037 Tromsø, Norway.
authors: Alena
authors: A
authors: Didriksen
authors: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors: Olga V
authors: OV
authors: Danilova
authors: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors: Svetlana E
authors: SE
authors: Belova
authors: GFZ German Research Centre for Geosciences, Section 4.5 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors: Susanne
authors: S
authors: Liebner
authors: UiT The Arctic University of Norway, Department of Arctic and Marine Biology, 9037 Tromsø, Norway.
authors: Mette M
authors: MM
authors: Svenning
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042062:Beijerinckiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008697:Methane / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009664:Norway; D010105:Oxygenases / Q000378:metabolism; D065946:Permafrost / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane; D000432:Methanol