title: Transduced Heme Oxygenase-1 Fusion Protein Reduces Renal Ischemia/Reperfusion Injury Through Its Antioxidant and Antiapoptotic Roles in Rats.
abstract: BACKGROUND
  Heme oxygenase-1 (HO-1) has a protective role against ischemia/reperfusion (I/R) injury.
  
  
  METHODS
  We produced an HO-1 fusion protein mediated by cell penetrated peptide PEP-1, also known as PEP-1-HO-1 fusion protein, and investigated its role in renal I/R injury in rats. Male Sprague-Dawley rats were subjected to 45 minutes of ischemia by occluding the bilateral renal arteries and 6 hours of reperfusion to prepare the model of renal I/R. Animals were randomized to receive PEP-1-HO-1 fusion protein or equal volume of physiologic saline 30 minutes before ischemia.
  
  
  RESULTS
  Administration of PEP-1-HO-1 fusion protein resulted in a significant increase in HO-1 expression. His-probe expression (1 part of the PEP-1-HO-1 fusion protein) was only observed in PEP-1-HO-1-treated animals. I/R caused renal dysfunction and increases in malondialdehyde level and cell apoptosis, and decreased superoxide dismutase activity. Treatment of PEP-1-HO-1 fusion protein reversed these changes. Furthermore, administration of PEP-1-HO-1 inhibited the I/R-induced increase in nuclear factor-κB activation.
  
  
  CONCLUSIONS
  These findings suggest that transduction of PEP-1-HO-1 attenuates renal I/R injury in rats, which might be partly attributable to its antioxidant and antiapoptotic effects.
authors: Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. Electronic address: hexh1220@gmail.com.
authors: X-H
authors: XH
authors: He
authors: Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors: J-J
authors: JJ
authors: Tang
authors: Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors: Y-L
authors: YL
authors: Wang
authors: Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors: Z-Z
authors: ZZ
authors: Zhang
authors: Department of Anesthesiology, Shenzhen Boan Maternity and Child Health Hospital, Shenzhen, China.
authors: X-T
authors: XT
authors: Yan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D004195:Disease Models, Animal; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000378:metabolism / Q000494:pharmacology*; D019194:Ischemic Preconditioning / Q000379:methods; D007668:Kidney / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; D008315:Malondialdehyde; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C561500:PEP-1-heme oxygenase-1 fusion protein