title: Optimization of an accelerated solvent extraction dispersive liquid-liquid microextraction method for the separation and determination of essential oil from Ligusticum chuanxiong Hort by gas chromatography with mass spectrometry.
abstract: In this study, an accelerated solvent extraction dispersive liquid-liquid microextraction coupled with gas chromatography and mass spectrometry was established and employed for the extraction, concentration and analysis of essential oil constituents from Ligusticum chuanxiong Hort. Response surface methodology was performed to optimize the key parameters in accelerated solvent extraction on the extraction efficiency, and key parameters in dispersive liquid-liquid microextraction were discussed as well. Two representative constituents in Ligusticum chuanxiong Hort, (Z)-ligustilide and n-butylphthalide, were quantitatively analyzed. It was shown that the qualitative result of the accelerated solvent extraction dispersive liquid-liquid microextraction approach was in good agreement with that of hydro-distillation, whereas the proposed approach took far less extraction time (30 min), consumed less plant material (usually <1 g, 0.01 g for this study) and solvent (<20 mL) than the conventional system. To sum up, the proposed method could be recommended as a new approach in the extraction and analysis of essential oil.
authors: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors: Guang
authors: G
authors: Yang
authors: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|School of Medicine, Tongji University, Shanghai, P. R. China.
authors: Qiushi
authors: Q
authors: Sun
authors: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors: Zhiyan
authors: Z
authors: Hu
authors: Experiment Center, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China.
authors: Hua
authors: H
authors: Liu
authors: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors: Tingting
authors: T
authors: Zhou
authors: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.|School of Medicine, Tongji University, Shanghai, P. R. China.
authors: Guorong
authors: G
authors: Fan
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D030004:Ligusticum; D059627:Liquid Phase Microextraction; D015394:Molecular Structure; D009822:Oils, Volatile / Q000032:analysis*; D012997:Solvents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D012997:Solvents; C101574:chuangxiong extract
keywords: Accelerated solvent extraction; Dispersive liquid-liquid microextraction; Gas chromatography with mass spectrometry; Ligusticum chuanxiong Hort