title: Condensin- and Replication-Mediated Bacterial Chromosome Folding and Origin Condensation Revealed by Hi-C and Super-resolution Imaging.
abstract: Chromosomes of a broad range of species, from bacteria to mammals, are structured by large topological domains whose precise functional roles and regulatory mechanisms remain elusive. Here, we combine super-resolution microscopies and chromosome-capture technologies to unravel the higher-order organization of the Bacillus subtilis chromosome and its dynamic rearrangements during the cell cycle. We decipher the fine 3D architecture of the origin domain, revealing folding motifs regulated by condensin-like complexes. This organization, along with global folding throughout the genome, is present before replication, disrupted by active DNA replication, and re-established thereafter. Single-cell analysis revealed a strict correspondence between sub-cellular localization of origin domains and their condensation state. Our results suggest that the precise 3D folding pattern of the origin domain plays a role in the regulation of replication initiation, chromosome organization, and DNA segregation.
authors: Institut Pasteur, Department of Genomes and Genetics, Group Spatial Regulation of Genomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France.
authors: Martial
authors: M
authors: Marbouty
authors: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
authors: Antoine
authors: A
authors: Le Gall
authors: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
authors: Diego I
authors: DI
authors: Cattoni
authors: Institut Pasteur, Department of Genomes and Genetics, Group Spatial Regulation of Genomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France.
authors: Axel
authors: A
authors: Cournac
authors: Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, NE2 4AX Newcastle Upon Tyne, UK.
authors: Alan
authors: A
authors: Koh
authors: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
authors: Jean-Bernard
authors: JB
authors: Fiche
authors: Laboratoire de Physique Théorique de la Matière Condensée, CNRS UMR 7600, Université Pierre et Marie Curie, Sorbonne Universités, 75005 Paris, France.
authors: Julien
authors: J
authors: Mozziconacci
authors: Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, NE2 4AX Newcastle Upon Tyne, UK.
authors: Heath
authors: H
authors: Murray
authors: Institut Pasteur, Department of Genomes and Genetics, Group Spatial Regulation of Genomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France. Electronic address: romain.koszul@pasteur.fr.
authors: Romain
authors: R
authors: Koszul
authors: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France. Electronic address: marcelo.nollmann@cbs.cnrs.fr.
authors: Marcelo
authors: M
authors: Nollmann
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004261:DNA Replication; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008853:Microscopy; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000378:metabolism*; D061848:Optical Imaging; D018741:Replication Origin
chemical_list: D001426:Bacterial Proteins; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C108312:condensin complexes; D000251:Adenosine Triphosphatases
keywords: DNA replication; bacterial mitosis; chromosome conformation capture; chromosome segregation; chromosome structure and organization; condensins; super-resolution microscopy