title: Effect of Yeast Cell Morphology, Cell Wall Physical Structure and Chemical Composition on Patulin Adsorption.
abstract: The capability of yeast to adsorb patulin in fruit juice can aid in substantially reducing the patulin toxic effect on human health. This study aimed to investigate the capability of yeast cell morphology and cell wall internal structure and composition to adsorb patulin. To compare different yeast cell morphologies, cell wall internal structure and composition, scanning electron microscope, transmission electron microscope and ion chromatography were used. The results indicated that patulin adsorption capability of yeast was influenced by cell surface areas, volume, and cell wall thickness, as well as 1,3-β-glucan content. Among these factors, cell wall thickness and 1,3-β-glucan content serve significant functions. The investigation revealed that patulin adsorption capability was mainly affected by the three-dimensional network structure of the cell wall composed of 1,3-β-glucan. Finally, patulin adsorption in commercial kiwi fruit juice was investigated, and the results indicated that yeast cells could adsorb patulin from commercial kiwi fruit juice efficiently. This study can potentially simulate in vitro cell walls to enhance patulin adsorption capability and successfully apply to fruit juice industry.
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Ying
authors: Y
authors: Luo
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Jianguo
authors: J
authors: Wang
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Bin
authors: B
authors: Liu
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Zhouli
authors: Z
authors: Wang
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Yahong
authors: Y
authors: Yuan
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Tianli
authors: T
authors: Yue
mesh_terms: D000327:Adsorption; D041022:Candida tropicalis / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D048429:Cell Size; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D062610:Fungal Polysaccharides / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D010365:Patulin / Q000378:metabolism*; D010843:Pichia / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D062610:Fungal Polysaccharides; D010365:Patulin