title: Aberrant expression of Notch1/numb/snail signaling, an epithelial mesenchymal transition related pathway, in adenomyosis.
abstract: BACKGROUND
  Epithelial mesenchymal transition (EMT) is involved in the pathogenesis of adenomyosis, and Notch signaling is crucial to EMT. The objective of this study was to explore Notch1/Numb/Snail signaling in adenomyosis.
  
  
  METHODS
  The expression levels of the members of the Notch1/Numb/Snail signaling cascade in normal endometria (proliferative phase: n = 15; secretory phase: n = 15; postmenopausal phase: n = 15) and adenomyotic endometria (proliferative phase: n = 15; secretory phase: n = 15) were determined by immunohistochemistry analysis.
  
  
  RESULTS
  We found that the expressions of Notch1 and the EMT-related proteins N-cadherin, Snail and Slug were upregulated in the ectopic endometrium of adenomyosis compared with normal endometrium. Numb, a negative regulator of Notch signaling, was significantly decreased in adenomyosis. In addition, reduced immunoexpression of E-cadherin was observed in adenomyosis.
  
  
  CONCLUSIONS
  We conclude that Notch1/Numb/Snail signaling plays an important role in the pathogenesis and development of adenomyosis.
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. shashaqi1029@163.com.
authors: Shasha
authors: S
authors: Qi
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. zxb0626@126.com.
authors: Xingbo
authors: X
authors: Zhao
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. limingjiang1963@126.com.
authors: Mingjiang
authors: M
authors: Li
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. qzyzzxh@163.com.
authors: Xiaohui
authors: X
authors: Zhang
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. lzz19890724@163.com.
authors: Zhenzhen
authors: Z
authors: Lu
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. ycr911@163.com.
authors: Chunrun
authors: C
authors: Yang
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. 15168886369@163.com.
authors: Chunhua
authors: C
authors: Zhang
authors: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. huizhang1218@126.com.
authors: Hui
authors: H
authors: Zhang
authors: Department of Anesthesiology and Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong, 250021, People's Republic of China. cinderella19811218@163.com.
authors: Na
authors: N
authors: Zhang
mesh_terms: D062788:Adenomyosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D051881:Receptor, Notch1 / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D009419:Nerve Tissue Proteins; C113026:NUMB protein, human; D051881:Receptor, Notch1; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors