title: PREVALENCE OF THE COMPLEMENT FACTOR H AND GSTM1 GENES POLYMORPHISMS IN PATIENTS WITH CENTRAL SEROUS CHORIORETINOPATHY.
abstract: OBJECTIVE
  The purpose of our study was to investigate the potential association between the complement factor H (CFH) (rs3753394, rs800292, rs2284664, rs1329428, and rs1065489) and GSTM1 gene polymorphisms, and central serous chorioretinopathy (CSCR) susceptibility in a well-defined Greek cohort.
  
  
  METHODS
  We enrolled a case-control study in a Greek population with 41 cases and 78 controls. Five milliliters of peripheral blood was collected from each participant, and DNA was extracted using the PureLink Genomic DNA kit. The CFH (rs3753394, rs800292, rs2284664, rs1329428, and rs1065489) single nucleotide polymorphisms and GSTM1 polymorphism were tested using polymerase chain reaction assays.
  
  
  RESULTS
  Between CSCR (n = 41) cases and controls (n = 78), the TT rs3753394, GG rs1329428, and TT rs1065489 genotypes' frequencies of the CFH gene were found to be significantly associated with risk of CSCR. The genotype frequency of the CFH rs2284664, rs800292, and of the GSTM1 gene polymorphisms was not found to be significantly associated with CSCR.
  
  
  CONCLUSIONS
  Our results demonstrated a significant association between CSCR and single nucleotide polymorphisms in the CFH gene (rs3753394, rs1329428, and rs1065489), suggesting that disturbances in choroidal vasculature, through intercorrelation with adrenomedullin, play a significant role in CSCR pathogenesis.
authors: *First Department of Ophthalmology, University of Athens, Athens, Greece; †Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, University of Athens, Athens, Greece; ‡Department of Ophthalmology, University Hospital of Geneva, Geneva, Switzerland; §Laser and Retinal Research Unit, King's College Hospital, London, United Kingdom; and ¶Second Department of Ophthalmology, Ophthalmiatrion Athinon, Athens, Greece.
authors: Marilita M
authors: MM
authors: Moschos
authors: Maria
authors: M
authors: Gazouli
authors: Zisis
authors: Z
authors: Gatzioufas
authors: Dimitrios
authors: D
authors: Brouzas
authors: Nikolaos
authors: N
authors: Nomikarios
authors: Sobha
authors: S
authors: Sivaprasad
authors: Panagiotis
authors: P
authors: Mitropoulos
authors: Irini P
authors: IP
authors: Chatziralli
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D056833:Central Serous Chorioretinopathy / Q000175:diagnosis / Q000235:genetics*; D017242:Complement Factor H / Q000235:genetics; D005260:Female; D005451:Fluorescein Angiography; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D041623:Tomography, Optical Coherence; D055815:Young Adult
chemical_list: D017242:Complement Factor H; D005982:Glutathione Transferase; C493495:CFH protein, human