title: New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy.
abstract: Ornithodoros moubata is the main vector of the pathogens causing African swine fever and human relapsing fever in Africa. The development of an efficient vaccine against this tick would facilitate its control and the prevention of the diseases it transmits to a considerable extent. Previous efforts to identify vaccine target candidates led us to the discovery of novel salivary proteins that probably act as anti-haemostatics at the host-tick interface, including a secreted phospholipase A2 (PLA2), a 7DB-like protein (7DB-like), a riboprotein 60S L10 (RP-60S), an apyrase (APY), and a new platelet aggregation inhibitor peptide, designated mougrin (MOU). In this work, the corresponding recombinant proteins were expressed in Escherichia coli and their individual vaccine efficacy was tested in rabbit vaccination trials. All of them, except the less immunogenic RP-60S, induced strong humoral responses that reduced tick feeding and survival, providing vaccine efficacies of 44.2%, 43.2% and 27.2%, 19.9% and 17.3% for PLA2, APY, MOU, RP-60S and 7DB-like, respectively. In the case of the more protective recombinant antigens (PLA2, APY and MOU), the immunodominant protective linear B-cell epitopes were identified and their combined vaccine efficacy was tested in a second vaccine trial using different adjuvants. In comparison with the best efficacy of individual antigens, the multicomponent vaccine increased vaccine efficacy by 13.6%, indicating additive protective effects rather than a synergistic effect. Tick saliva inoculated during natural tick-host contacts had a boosting effect on vaccinated animals, increasing specific antibody levels and protection.
authors: Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain. Electronic address: veronicadiazmartin@gmail.com.
authors: Verónica
authors: V
authors: Díaz-Martín
authors: Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain. Electronic address: raul.manzano@irnasa.csic.es.
authors: Raúl
authors: R
authors: Manzano-Román
authors: Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain. Electronic address: ana.oleaga@irnasa.csic.es.
authors: Ana
authors: A
authors: Oleaga
authors: Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain. Electronic address: ricardo.perez@irnasa.csic.es.
authors: Ricardo
authors: R
authors: Pérez-Sánchez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology; D003001:Cloning, Molecular; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006490:Hemostatics / Q000037:antagonists & inhibitors*; D007117:Immunization, Secondary; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D026861:Ornithodoros / Q000276:immunology / Q000378:metabolism*; D011487:Protein Conformation; D011506:Proteins / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000941:Antigens; D018985:Epitopes, B-Lymphocyte; D006490:Hemostatics; D011506:Proteins; D011994:Recombinant Proteins; D014612:Vaccines
keywords: B-cell epitopes; Ornithodoros moubata; Recombinant antigens; Salivary antigens; Vaccine