title: Identifying novel protein interactions: Proteomic methods, optimisation approaches and data analysis pipelines.
abstract: The technological revolution in high-throughput nucleic acid and protein analysis in the last 15 years has launched the field of 'omics' and led to great advances in our understanding of cell biology. Consequently the study of the cellular proteome and protein dynamics, in particular interactomics, has been a matter of intense investigation, specifically the determination and description of complex protein interaction networks in the cell, not only with other proteins but also with RNA and DNA. The analysis of these interactions, beginning with their identification and ultimately resulting in structural level examination, is one of the cornerstones of modern biological science underpinning basic research and impacting on applied biology, biomedicine and drug discovery. In this review we summarise a selection of emerging and established techniques currently being applied in this field with a particular focus on affinity-based purification systems and their optimisation, including tandem affinity purification (TAP) tagging, isolation of proteins on nascent DNA (IPOND) and RNA-protein immunoprecipitation in tandem (RIPiT). The recent application of quantitative proteomics to improve stringency and specificity is also discussed, including the use of metabolic labelling by stable isotope labelling by amino acids in cell culture (SILAC), localization of organelle proteins by isotope tagging (LOPIT) and proximity-dependent biotin identification (BioID). Finally, we describe a range of software resources that can be applied to interactomics, both to handle raw data and also to scrutinise its broader biological context. In this section we focus especially on open-access online interactomic databases such as Reactome and IntAct.
authors: College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors: Daniel Gonçalves
authors: DG
authors: Carneiro
authors: College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors: Thomas
authors: T
authors: Clarke
authors: College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. Electronic address: c.c.davies@bham.ac.uk.
authors: Clare C
authors: CC
authors: Davies
authors: College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. Electronic address: d.bailey@bham.ac.uk.
authors: Dalan
authors: D
authors: Bailey
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods; D002846:Chromatography, Affinity; D004247:DNA / Q000032:analysis / Q000378:metabolism; D030562:Databases, Protein; D047468:Immunoprecipitation; D007553:Isotope Labeling; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000378:metabolism; D012984:Software; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000596:Amino Acids; D020543:Proteome; D012313:RNA; D004247:DNA
keywords: Affinity purification; DNA–protein interactions; Interactomics; Protein–protein interaction; RNA–protein interaction; TAP-tagging