title: Altered expression of LINC-ROR in cancer cell lines and tissues.
abstract: According to GLOBOCAN 2012, the worldwide burden of cancer increased and is expected to worsen within the next decades. Therefore, universal combat against cancer will not succeed with treatment solely; effective prevention and early detection are urgently needed to tackle the cancer crisis. Emerging data demonstrate that long non-coding RNAs are involved in numerous biological and pathological processes like development and differentiation and in a variety of human diseases including cancer. Located at 18q21, LINC-ROR (regulator of reprogramming) is a modulator of ESCs maintenance and hypoxia-signaling pathways in hepatocellular cancer cells. The aim of this study was to examine the expression of LINC-ROR in various cell lines and representative samples of human cancers by quantitative real-time RT-PCR to provide a snapshot on how LINC-ROR expression may be deregulated in cancer. More than 30 cell lines and 112 patient specimens from various tissues were assessed for relative expression of LINC-ROR. Our results revealed that the expression of LINC-ROR was lower in all somatic cancer cell lines compared to stem cells or cells with stem cell-like capabilities, like the embryonic carcinoma cell line, NTERA-2. In tissues, expression patterns vary, but some cancerous tissues displayed increased LINC-ROR expression compared to corresponding normal tissues. Thus, we hypothesize that LINC-ROR may have a key function in a subpopulation of cells from the tumor bulk, i.e., the cancer stem cells associated with specific properties including resistance to adverse environmental conditions.
authors: Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.|Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Majdaddin
authors: M
authors: Rezaei
authors: Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.|Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
authors: Modjtaba
authors: M
authors: Emadi-Baygi
authors: Department of Urology, Medical Faculty of the Heinrich-Heine-University, Düsseldorf, Germany.
authors: Michèle J
authors: MJ
authors: Hoffmann
authors: Department of Urology, Medical Faculty of the Heinrich-Heine-University, Düsseldorf, Germany.
authors: Wolfgang A
authors: WA
authors: Schulz
authors: Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran. pnikpour@med.mui.ac.ir.|Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. pnikpour@med.mui.ac.ir.|Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. pnikpour@med.mui.ac.ir.
authors: Parvaneh
authors: P
authors: Nikpour
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D065150:Cellular Reprogramming / Q000235:genetics; D057809:HEK293 Cells; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000860:Hypoxia / Q000235:genetics; D061986:MCF-7 Cells; D009369:Neoplasms / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding
keywords: Cancer; Gene expression; LINC-ROR; Long non-coding RNAs