title: Two NAC transcription factors from Caragana intermedia altered salt tolerance of the transgenic Arabidopsis.
abstract: BACKGROUND
  Plants are continuously challenged by different environment stresses, and they vary widely in their adjustability. NAC (NAM, ATAF and CUC) transcription factors are known to be crucial in plants tolerance response to abiotic stresses, such as drought and salinity. ANAC019, ANAC055, and ANAC072, belong to the stress-NAC TFs, confer the Arabidopsis abiotic stress tolerance.
  
  
  RESULTS
  Here we isolated two stress-responsive NACs, CiNAC3 and CiNAC4, from Caragana intermedia, which were induced by ABA and various abiotic stresses. Localization assays revealed that CiNAC3 and CiNAC4 localized in the nuclei, consistent with their roles as transcription factors. Histochemistry assay using Pro(CiNAC4)::GUS transgenic Arabidopsis showed that the expression of the GUS reporter was observed in many tissues of the transgenic plants, especially in the root vascular system. Overexpression of CiNAC3 and CiNAC4 reduced ABA sensitivity during seed germination, and enhanced salt tolerance of the transgenic Arabidopsis.
  
  
  CONCLUSIONS
  We characterised CiNAC3 and CiNAC4 and found that they were induced by numerous abiotic stresses and ABA. GUS histochemical assay of CiNAC4 promoter suggested that root, flower and local damaged tissues were the strongest stained tissues. Overexpression assay revealed that CiNAC4 play essential roles not only in promoting lateral roots formation, but also in responding to salinity and ABA treatment of Arabidopsis.
authors: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China. hanxiaominhushi@163.com.
authors: Xiaomin
authors: X
authors: Han
authors: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China. fengzongqier@163.com.
authors: Zongqi
authors: Z
authors: Feng
authors: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China. dan2008132128@126.com.
authors: Dandan
authors: D
authors: Xing
authors: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China. atp_yangqi@163.com.
authors: Qi
authors: Q
authors: Yang
authors: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China. wangruigang@imau.edu.cn.
authors: Ruigang
authors: R
authors: Wang
authors: Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, P. R. China. lwqi@caf.ac.cn.
authors: Liwang
authors: L
authors: Qi
authors: College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China. liguojing@imau.edu.cn.
authors: Guojing
authors: G
authors: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D032602:Caragana / Q000187:drug effects / Q000378:metabolism*; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid