title: Evaluation of four chromogenic media for the isolation of Group B Streptococcus from vaginal specimens in pregnant women.
abstract: Direct culture onto four commercial chromogenic media, selective for the isolation of Group B Streptococcus (GBS), were compared with the conventional pre-enrichment Centers for Disease Control and Prevention (CDC) method for the ability to isolate GBS from 242 pregnant women's self-collected vaginal/perineal swabs. The sensitivities and specificities for direct culture on to chromogenic agar were 92% and 100% for StrepBSelect (Bio-Rad Laboratories), 96% and 100% for Brilliance GBS (Thermo-Fisher Scientific), 94% and 100% for CHROMagar StrepB (CHROMagar, Dutec Diagnostics), 86% and 100% for ChromID Strepto B (bioMerieux). CDC recommended broth pre-enrichment then culture on blood containing selective agar had a sensitivity and specificity of 90.0% and 100% respectively. The chromogenic agar tested produced comparable results to the pre-enrichment CDC method.
authors: Austin Pathology, Microbiology Department, Heidelberg, Vic, Australia.
authors: Nahim
authors: N
authors: Salem
authors: Jeffrey J
authors: JJ
authors: Anderson
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002863:Chromogenic Compounds; D003470:Culture Media; D005260:Female; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis*; D013292:Streptococcus agalactiae; D014626:Vaginal Smears
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media