title: The anti-tumor effect of a polypeptide extracted from Tegillarca granosa Linnaeus on renal metastatic tumor OS-RC-2 cells.
abstract: BACKGROUND
  Renal cell carcinoma is a type of malignant tumor often diagnosed in the urinary system. The aim of this study is to evaluate the anti-tumor effects and mechanisms of a polypeptide, Haishengsu (TG-1), with different concentrations (125, 250, 500 mg/kg) on renal metastatic tumor OS-RC-2 cells.
  
  
  METHODS
  We first established the renal metastatic tumor model. After being administered with TG-1, the weight of tumors was measured and the microstructural changes of renal carcinoma OS-RC-2 cells were compared using transmission electron microscopy before and after the therapy. The Ki67 expression in renal carcinoma OS-RC-2 cells was analyzed by RT-PCR and downstream signal molecule caspase-3 was measured by Western blot assay.
  
  
  RESULTS
  After treatment with different doses of TG-1, the tumor weights in the positive control group and experimental groups were smaller than those in the untreated control group, suggesting that TG-1 could effectively inhibit tumor growth in mice. The transmission electron microscopy and flow cytometry results indicated that TG-1 induces tumor cell apoptosis (p < 0.05). The tumor cells exhibited polymorphism changes and chromatin edge clustering. TG-1 also inhibited Ki67 expression and promoted caspase-3 expression in the tumor significantly (p < 0.05).
  
  
  CONCLUSIONS
  TG-1 inhibits the growth of the tumor and induces apoptosis of the tumor cells by inducing caspase-3 expression. The results provide a basis for clinical application of TG-1 in the future.
authors: The Medical College of Qingdao University, Qingdao, China.
authors: Wenhua
authors: W
authors: Xu
authors: Qingdao Agricultural University, Qingdao, China.
authors: Xiaoying
authors: X
authors: Kong
authors: Department of Pathology, Municipal Hospital of Qingdao, Qingdao, China.
authors: Changqing
authors: C
authors: Jiang
authors: The Garrison Command Hospital of Qingdao, Qindao, China.
authors: Xiaoyan
authors: X
authors: Liu
authors: The Medical College of Qingdao University, Qingdao, China.
authors: Luo
authors: L
authors: Xu
keywords: Ki67 mRNA; anti-tumor effect; polypeptide; renal tumor OS-RC-2 cell