title: Enhancing the performance of LC-MS for intact protein analysis by counteracting the signal suppression effects of trifluoroacetic acid during electrospray.
abstract: We develop an acidic vapor assisted electrospray ionization strategy within an enclosed electrospray ionization source to counteract the ion suppression effects caused by trifluoroacetic acid. The mass spectrometry signal intensity of intact proteins was improved 10 times and the number of valid signals for E. coli intact protein samples was improved 96% by using this strategy.
authors: Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China. wangfj@dicp.ac.cn hanfazou@dicp.ac.cn.
authors: Jin
authors: J
authors: Chen
authors: Zheyi
authors: Z
authors: Liu
authors: Fangjun
authors: F
authors: Wang
authors: Jiawei
authors: J
authors: Mao
authors: Ye
authors: Y
authors: Zhou
authors: Jing
authors: J
authors: Liu
authors: Hanfa
authors: H
authors: Zou
authors: Yukui
authors: Y
authors: Zhang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D014269:Trifluoroacetic Acid / Q000737:chemistry*
chemical_list: D011506:Proteins; D014269:Trifluoroacetic Acid