title: Resveratrol inhibits rhinovirus replication and expression of inflammatory mediators in nasal epithelia.
abstract: Human rhinoviruses (HRV), the cause of common colds, are the most frequent precipitants of acute exacerbation of asthma and chronic obstructive pulmonary disease, as well as causes of other serious respiratory diseases. No vaccine or antiviral agents are available for the prevention or treatment of HRV infection. Resveratrol exerts antiviral effect against different DNA and RNA viruses. The antiviral effect of a new resveratrol formulation containing carboxymethylated glucan was analyzed in H1HeLa cell monolayers and ex vivo nasal epithelia infected with HRV-16. Virus yield was evaluated by plaque assay and expression of viral capsid proteins by Western blot. IL-10, IFN-β, IL-6, IL-8 and RANTES levels were evaluated by ELISA assay. ICAM-1 was assessed by Western blot and immunofluorescence. Resveratrol exerted a high, dose-dependent, antiviral activity against HRV-16 replication and reduced virus-induced secretion of IL-6, IL-8 and RANTES to levels similar to that of uninfected nasal epithelia. Basal levels of IL-6 and RANTES were also significantly reduced in uninfected epithelia confirming an anti-inflammatory effect of the compound. HRV-induced expression of ICAM-1 was reversed by resveratrol. Resveratrol may be useful for a therapeutic approach to reduce HRV replication and virus-induced cytokine/chemokine production.
authors: Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University, I-00185 Rome, Italy. Electronic address: paola.mastromarino@uniroma1.it.
authors: Paola
authors: P
authors: Mastromarino
authors: Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University, I-00185 Rome, Italy.
authors: Daniela
authors: D
authors: Capobianco
authors: Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University, I-00185 Rome, Italy.
authors: Federica
authors: F
authors: Cannata
authors: Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University, I-00185 Rome, Italy.
authors: Chiara
authors: C
authors: Nardis
authors: Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University, I-00185 Rome, Italy.
authors: Elena
authors: E
authors: Mattia
authors: Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University, I-00185 Rome, Italy.
authors: Alessandra
authors: A
authors: De Leo
authors: NOOS s.r.l., I-00181 Rome, Italy.
authors: Rossella
authors: R
authors: Restignoli
authors: Department of Biochemical Sciences, Sapienza University, I-00185 Rome, Italy.
authors: Antonio
authors: A
authors: Francioso
authors: Department of Biochemical Sciences, Sapienza University, I-00185 Rome, Italy.
authors: Luciana
authors: L
authors: Mosca
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D015153:Blotting, Western; D016207:Cytokines / Q000037:antagonists & inhibitors*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D009297:Nasal Mucosa; D009924:Organ Culture Techniques; D000077185:Resveratrol; D012229:Rhinovirus / Q000187:drug effects* / Q000502:physiology*; D013267:Stilbenes / Q000494:pharmacology*; D019562:Viral Load; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D016207:Cytokines; D013267:Stilbenes; D000077185:Resveratrol
keywords: Human rhinovirus; Inflammatory mediators; Nasal epithelia; Resveratrol