title: Identification of Drosophila centromere associated proteins by quantitative affinity purification-mass spectrometry.
abstract: Centromeres of higher eukaryotes are epigenetically defined by the centromere specific histone H3 variant CENP-A(CID). CENP-A(CID) builds the foundation for the assembly of a large network of proteins. In contrast to mammalian systems, the protein composition of Drosophila centromeres has not been comprehensively investigated. Here we describe the proteome of Drosophila melanogaster centromeres as analyzed by quantitative affinity purification-mass spectrometry (AP-MS). The AP-MS input chromatin material was prepared from D. melanogaster cell lines expressing CENP-A(CID) or H3.3 fused to EGFP as baits. Centromere chromatin enriched proteins were identified based on their relative abundance in CENP-A(CID)-GFP compared to H3.3-GFP or mock affinity-purifications. The analysis yielded 86 proteins specifically enriched in centromere chromatin preparations. The data accompanying the manuscript on this approach (Barth et al., 2015, Proteomics 14:2167-78, DOI: 10.1002/pmic.201400052) has been deposited to the ProteomeXchange Consortium (http://www.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD000758.
authors: Munich Center of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig-Maximilians-University of Munich, 80336 Munich, Germany.
authors: Teresa K
authors: TK
authors: Barth
authors: Max-Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
authors: Georg O M
authors: GO
authors: Schade
authors: Munich Center of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig-Maximilians-University of Munich, 80336 Munich, Germany.
authors: Andreas
authors: A
authors: Schmidt
authors: Munich Center of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig-Maximilians-University of Munich, 80336 Munich, Germany.
authors: Irene
authors: I
authors: Vetter
authors: Munich Center of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig-Maximilians-University of Munich, 80336 Munich, Germany.
authors: Marc
authors: M
authors: Wirth
authors: Max-Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
authors: Patrick
authors: P
authors: Heun
authors: Munich Center of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig-Maximilians-University of Munich, 80336 Munich, Germany.
authors: Axel
authors: A
authors: Imhof
authors: Munich Center of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig-Maximilians-University of Munich, 80336 Munich, Germany.
authors: Andreas W
authors: AW
authors: Thomae
keywords: CENP-A; CID; Centromere; Chromatin proteomics; Drosophila