title: Quercetin reduced inflammation and increased antioxidant defense in rat adjuvant arthritis.
abstract: Novel therapies for rheumatoid arthritis also include the use of naturally occurring compounds possessing antioxidant properties. In the present work, the effects of oral administration of quercetin were investigated in a rat model of adjuvant arthritis. Arthritis was induced by a single intradermal injection of heat-inactivated Mycobacterium butyricum in incomplete Freund's adjuvant. The experimental groups were treated with an oral daily dose of 150 mg/kg b.w. of quercetin for 28 days. Results indicated that quercetin was able to ameliorate all markers of inflammation and oxidative stress measured. Quercetin lowered levels of interleukin-1β, C-reactive protein, and monocyte chemotactic protein-1 and restored plasma antioxidant capacity. In addition, quercetin inhibited the enzymatic activity of pro-inflammatory 12/15-lipoxygenase in lung and liver and increased the expression of heme oxygenase-1 in joint and lung of arthritic rats. Finally, quercetin inhibited the 2-fold increase of NF-қB activity observed in lung, liver and joint after induction of arthritis.
authors: Institute of Food Sciences, National Research Council, 83100 Avellino, Italy; Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro, I-53100 Siena, Italy. Electronic address: concetta.gardi@unisi.it.
authors: C
authors: C
authors: Gardi
authors: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska Cesta 9, SK-841 04 Bratislava, Slovak Republic; Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
authors: K
authors: K
authors: Bauerova
authors: Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro, I-53100 Siena, Italy.
authors: B
authors: B
authors: Stringa
authors: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska Cesta 9, SK-841 04 Bratislava, Slovak Republic.
authors: V
authors: V
authors: Kuncirova
authors: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska Cesta 9, SK-841 04 Bratislava, Slovak Republic.
authors: L
authors: L
authors: Slovak
authors: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska Cesta 9, SK-841 04 Bratislava, Slovak Republic.
authors: S
authors: S
authors: Ponist
authors: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska Cesta 9, SK-841 04 Bratislava, Slovak Republic.
authors: F
authors: F
authors: Drafi
authors: Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
authors: L
authors: L
authors: Bezakova
authors: Institute of Food Sciences, National Research Council, 83100 Avellino, Italy.
authors: I
authors: I
authors: Tedesco
authors: Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro, I-53100 Siena, Italy.
authors: A
authors: A
authors: Acquaviva
authors: Institute of Food Sciences, National Research Council, 83100 Avellino, Italy.
authors: S
authors: S
authors: Bilotto
authors: Institute of Food Sciences, National Research Council, 83100 Avellino, Italy. Electronic address: glrusso@isa.cnr.it.
authors: G L
authors: GL
authors: Russo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D007249:Inflammation / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D058945:Lipoxygenases / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D016328:NF-kappa B / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D011794:Quercetin; D058945:Lipoxygenases
keywords: Adjuvant arthritis; ERK pathway; Inflammation; NF-κB; Oxidative stress; Quercetin