title: Recording axonal conduction to evaluate the integration of pluripotent cell-derived neurons into a neuronal network.
abstract: Stem cell transplantation is a promising therapy to treat neurodegenerative disorders, and a number of in vitro models have been developed for studying interactions between grafted neurons and the host neuronal network to promote drug discovery. However, methods capable of evaluating the process by which stem cells integrate into the host neuronal network are lacking. In this study, we applied an axonal conduction-based analysis to a co-culture study of primary and differentiated neurons. Mouse cortical neurons and neuronal cells differentiated from P19 embryonal carcinoma cells, a model for early neural differentiation of pluripotent stem cells, were co-cultured in a microfabricated device. The somata of these cells were separated by the co-culture device, but their axons were able to elongate through microtunnels and then form synaptic contacts. Propagating action potentials were recorded from these axons by microelectrodes embedded at the bottom of the microtunnels and sorted into clusters representing individual axons. While the number of axons of cortical neurons increased until 14 days in vitro and then decreased, those of P19 neurons increased throughout the culture period. Network burst analysis showed that P19 neurons participated in approximately 80% of the bursting activity after 14 days in vitro. Interestingly, the axonal conduction delay of P19 neurons was significantly greater than that of cortical neurons, suggesting that there are some physiological differences in their axons. These results suggest that our method is feasible to evaluate the process by which stem cell-derived neurons integrate into a host neuronal network.
authors: Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, University of Tokyo, Room 1122, Faculty of Engineering Bldg., 14, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan, shimba@neuron.t.u-tokyo.ac.jp.
authors: Kenta
authors: K
authors: Shimba
authors: Koji
authors: K
authors: Sakai
authors: Yuzo
authors: Y
authors: Takayama
authors: Kiyoshi
authors: K
authors: Kotani
authors: Yasuhiko
authors: Y
authors: Jimbo
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D051379:Mice; D009415:Nerve Net / Q000166:cytology / Q000502:physiology*; D009431:Neural Conduction / Q000502:physiology*; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*