title: MicroRNA-454 functions as an oncogene by regulating PTEN in uveal melanoma.
abstract: MicroRNAs (miRNAs) regulate gene expression by targeted repression of transcription and translation, and are involved in carcinogenesis. In this study, we demonstrated that the expression of miR-454 was up-regulated in uveal melanoma tissues compared to normal tissues. Ectopic expression of miR-454 resulted in significant promotion of cell proliferation, colony formation, invasion and induction of cell cycle in uveal melanoma cells. Furthermore, we identified PTEN as a direct target of miR-454. Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells. These findings support an oncogene role of miR-454 in development of uveal melanoma.
authors: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors: Lei
authors: L
authors: Sun
authors: Department of Ophthalmology, The Second People's Hospital of Jinan, Jinan 250022, Shandong, China.
authors: Qiaoling
authors: Q
authors: Wang
authors: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors: Xiangchun
authors: X
authors: Gao
authors: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors: Dejing
authors: D
authors: Shi
authors: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors: Shuyong
authors: S
authors: Mi
authors: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. Electronic address: widedoor@yeah.net.
authors: Qing
authors: Q
authors: Han
mesh_terms: D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009857:Oncogenes; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics; D014604:Uveal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000098943:Uveal Melanoma
chemical_list: C583712:MIRN454 microRNA, human; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: MicroRNA; PTEN; Uveal melanoma; miR-454