title: Tectonic‑1 contributes to the growth and migration of prostate cancer cells in vitro.
abstract: Tectonic‑1 (TCTN1) is an upstream gene involved in embryonic development. The aim of the present study was to investigate the effect of the TCTN1 gene on the viability and migration of prostate cancer cells. Lentivirus‑mediated short hairpin RNA (shRNA) was constructed to silence the expression of TCTN1 in PC‑3 and DU145 prostate cancer cells. Cell viability and proliferation were measured using MTT and colony formation assays, and the distribution of cells in phases of the cell cycle was determined using flow cytometry. Cell migration was detected using a Transwell assay. The results demonstrated that TCTN1 was widely expressed in several human prostate cancer cell lines. Knockdown of the TCTN1 gene by RNA interference markedly suppressed cell viability and colony formation in the PC‑3 and DU145 cell lines. Cell cycle progression was also arrested by TCTN1 silencing. In addition, knockdown of the TCTN1 gene led to the inhibition of cell migration in the two cell lines. These findings confirmed the direct association between the TCTN1 gene and prostate cancer growth in vitro. With further understanding and clinical investigation, this indicates the potential for future development of a novel marker for early detection and gene therapy for prostate cancer.
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Zhijun
authors: Z
authors: Wang
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Yi
authors: Y
authors: Gao
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Yushan
authors: Y
authors: Liu
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Jie
authors: J
authors: Chen
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Junkai
authors: J
authors: Wang
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Sishun
authors: S
authors: Gan
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Danfeng
authors: D
authors: Xu
authors: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.
authors: Xingang
authors: X
authors: Cui
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D008565:Membrane Proteins; D009363:Neoplasm Proteins; C561486:Tctn1 protein, human