title: Clinical evaluation of a helicase-dependant amplification (HDA)-based commercial assay for the simultaneous detection of HSV-1 and HSV-2.
abstract: In this study, we evaluate the performance of a commercial assay, the AmpliVue HSV 1+2 Assay (Quidel), which employs HDA for the detection of both HSV-1 and HSV-2. The assay was tested on 307 clinical specimens (genital, oral, and dermal). When compared to shell vial virus culture and immunofluorescence typing of HSV, the positive percent agreement and negative percent agreement values were 98.2% and 90.9%, respectively. Excellent assay performance was demonstrated.
authors: Division of Microbiology, Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore. Electronic address: Jeanette_Teo@nuhs.edu.sg.
authors: Jeanette W P
authors: JW
authors: Teo
authors: Division of Microbiology, Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore.
authors: Donald
authors: D
authors: Chiang
authors: Division of Microbiology, Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore.
authors: Roland
authors: R
authors: Jureen
authors: Division of Microbiology, Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore.
authors: Raymond T P
authors: RT
authors: Lin
mesh_terms: D004265:DNA Helicases / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D004265:DNA Helicases
keywords: Helicase-dependant amplification; Herpesviridae; Molecular diagnostics