title: Novel fabrication of fluorescent silk utilized in biotechnological and medical applications.
abstract: Silk fibroin (SF) is a natural polymer widely used and studied for diverse applications in the biomedical field. Recently, genetically modified silks, particularly fluorescent SF fibers, were reported to have been produced from transgenic silkworms. However, they are currently limited to textile manufacturing. To expand the use of transgenic silkworms for biomedical applications, a solution form of fluorescent SF needed to be developed. Here, we describe a novel method of preparing a fluorescent SF solution and demonstrate long-term fluorescent function up to one year after subcutaneous insertion. We also show that fluorescent SF labeled p53 antibodies clearly identify HeLa cells, indicating the applicability of fluorescent SF to cancer detection and bio-imaging. Furthermore, we demonstrate the intraoperative use of fluorescent SF in an animal model to detect a small esophageal perforation (0.5 mm). This study suggests how fluorescent SF biomaterials can be applied in biotechnology and clinical medicine.
authors: Nano-Bio Regenerative Medical Institute, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702, Republic of Korea.
authors: Dong Wook
authors: DW
authors: Kim
authors: Nano-Bio Regenerative Medical Institute, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702, Republic of Korea.
authors: Ok Joo
authors: OJ
authors: Lee
authors: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Seong-Wan
authors: SW
authors: Kim
authors: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea.
authors: Chang Seok
authors: CS
authors: Ki
authors: School of Medicine, George Washington University, Washington, D.C., 20037, USA.
authors: Janet Ren
authors: JR
authors: Chao
authors: Department of Chemistry, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702, Republic of Korea.
authors: Hyojong
authors: H
authors: Yoo
authors: Department of Systems Immunology, School of Biomedical Sciences, Kangwon National University, 1, Kangwondaehak-gil, Chuncheon, Gangwon-do, 200-701, Republic of Korea.
authors: Sung-Il
authors: SI
authors: Yoon
authors: Nano-Bio Regenerative Medical Institute, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702, Republic of Korea.
authors: Jeong Eun
authors: JE
authors: Lee
authors: Nano-Bio Regenerative Medical Institute, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702, Republic of Korea.
authors: Ye Ri
authors: YR
authors: Park
authors: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: HaeYong
authors: H
authors: Kweon
authors: Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Kwang Gill
authors: KG
authors: Lee
authors: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors: David L
authors: DL
authors: Kaplan
authors: Nano-Bio Regenerative Medical Institute, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702, Republic of Korea; Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, 77, Sakju-ro, Chuncheon, Gangwon-do, 200-704, Republic of Korea. Electronic address: hlpch@paran.com.
authors: Chan Hum
authors: CH
authors: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D020811:Biomedical Technology / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D005349:Fibroins; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology; D047011:Silk / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051598:Whole Body Imaging
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D016159:Tumor Suppressor Protein p53; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005349:Fibroins
keywords: Biomaterials; Fluorescent; Medical application; Silk fibroin