title: [Efficacy of Nonmyeloablative Allogeneic Hematopoietic Stem Cells for 14 Case of Severe Acquired Aplastic Anemia].
abstract: OBJECTIVE
  To investigate the therapeutic efficacy of nonmyeloablative allogeneic hematopoietic stem cells transplantation for severe acquired aplastic anemia (SAA).
  
  
  METHODS
  Fourteen patients with severe acquired aplastic anemia received nonmyeloablative allogeneic hematopoietic stem cells transplantation from HLA matched sibling donors, among them 8 cases were dagnosed as SAA-I, 6 cases were diagnosed as SAA-II. The conditioning regimen consisted of fludarabine (FIUD), cyclophosphamide (CTX) and anti-thymocyte globulin (ATG/ALG). The prophylaxis for graft-versus-host disease (GVHD) was performed with cyclosporine (CsA) combined with mycophenolate mofetil (MMF) or tacrolimus (FK506).
  
  
  RESULTS
  All the patients gained a quick successfully engraftment of donor hametopoietic cells. The mean recovery time for neutrophil and platelet was 9 d and 13 d respectively. All the patients have acquired a full donor chimerism before 14 d. There were only 2 cases of GVHD: one out of them was acute skin GVHD (grade I) at day 70 after transplantation and the other was chronic liver GVHD (grade I) in 1 years after transplantation, the GVHD more than degree II did not coccur in all patients, 9 patients with bacterial and fungal mixed infection and (or) virus infection were observed, and improved after anti-infection therapy. The median follow-up time were 54.5 months (ranged between 5-144 months), and 12 patients remain disease-free survival currently, only 2 patients died of fungal infectin.
  
  
  CONCLUSIONS
  Transplantation of nonmyeloablative allogeneic hematopoietic stem cell is safe and effective for the treatment of severe acquired aplastic, but the prevention, treatment and monitoring of infection need to be enhance.
authors: School of Postgraduate, Anhui Medical University, Hefei 230032, Anhui Province, China.|Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Shao-Fei
authors: SF
authors: Wang
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Ya-Jing
authors: YJ
authors: Huang
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Chang-Lin
authors: CL
authors: Yu
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Qi-Yun
authors: QY
authors: Sun
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Jian-Hui
authors: JH
authors: Qiao
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Kai-Xun
authors: KX
authors: Hu
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Hong-Li
authors: HL
authors: Zuo
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Zheng
authors: Z
authors: Dong
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Zhi-Qing
authors: ZQ
authors: Liu
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Yi
authors: Y
authors: Wang
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Xiao-Mei
authors: XM
authors: Huang
authors: Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors: Hui-Sheng
authors: HS
authors: Ai
authors: School of Postgraduate, Anhui Medical University, Hefei 230032, Anhui Province, China.|Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. E-mail: guom196801@aliyun.com.
authors: Mei
authors: M
authors: Guo
mesh_terms: D064591:Allografts; D000741:Anemia, Aplastic; D000961:Antilymphocyte Serum; D003520:Cyclophosphamide; D016572:Cyclosporine; D018572:Disease-Free Survival; D006086:Graft vs Host Disease; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D009173:Mycophenolic Acid / Q000031:analogs & derivatives; D009504:Neutrophils; D035781:Siblings; D014019:Tissue Donors; D019172:Transplantation Conditioning; D014740:Vidarabine / Q000031:analogs & derivatives
chemical_list: D000961:Antilymphocyte Serum; D016572:Cyclosporine; D003520:Cyclophosphamide; D014740:Vidarabine; D009173:Mycophenolic Acid; C024352:fludarabine