title: Spinal cord injury in rats treated using bone marrow mesenchymal stem-cell transplantation.
abstract: The aim of this study was to observe the effects of bone marrow mesenchymal stem-cell transplantation (BMSCs) in repairing acute spinal cord damage in rats and to examine the potential beneficial effects. 192 Wistar rats were randomized into 8 groups. Spinal cord injury was created. Behavior and limb functions were scored. Repairing effects of BMSCs transplantation was evaluated and compared. In vitro 4',6-diamidino-2-phenylindole (DAPI)-tagged BMSCs were observed, and whether they migrated to the area of spinal cord injury after intravenous tail injection was investigated. The expression of neuron-specific protein (NSE) on BMSCs was examined. Fifteen days after transplantation, the BMSCs-treated groups scored significantly higher in limb function tests than the untreated group. Pathological sections of the bone marrow after operation showed significant recovery in treated groups in comparison to the control group. After transplantation, small amounts of fluorescent-tagged BMSCs can be found in the blood vessels in the area of spinal cord injury, and fluorescent-tagged BMSCs were diffused in extravascular tissues, whereas the DAPI-tagged BMSCs could not be detected,and BrdU/NSE double-labeled cells were found in the injured marrow. BMSCs improve behavioral responses and can repair spinal cord injuries by migrating to the injured area, where they can differentiate into neurons.
authors: The Second Hospital of Jilin University Changchun 130062, China.
authors: Yu-Bing
authors: YB
authors: Chen
authors: The No. 208 Hospital of The People's Liberation Army Changchun 130062, China.
authors: Quan-Zhang
authors: QZ
authors: Jia
authors: Medical School of Yanbian University Changchun 130062, China.
authors: Dong-Jun
authors: DJ
authors: Li
authors: The No. 208 Hospital of The People's Liberation Army Changchun 130062, China.
authors: Jing-Hai
authors: JH
authors: Sun
authors: The No. 208 Hospital of The People's Liberation Army Changchun 130062, China.
authors: Shuang
authors: S
authors: Xi
authors: The No. 208 Hospital of The People's Liberation Army Changchun 130062, China.
authors: Li-Ping
authors: LP
authors: Liu
authors: The No. 208 Hospital of The People's Liberation Army Changchun 130062, China.
authors: De-Xuan
authors: DX
authors: Gao
authors: The No. 208 Hospital of The People's Liberation Army Changchun 130062, China.
authors: Da-Wei
authors: DW
authors: Jiang
keywords: Bone marrow-derived mesenchymal stem cells; mechanism; spinal cord injury; transplantation