title: Differential expression of the chemosensory transcriptome in two populations of the stemborer Sesamia nonagrioides.
abstract: Among the proposed mechanisms of local adaptation to different ecological environments, transcriptional changes may play an important role. In this study, we investigated whether such variability occurred within the chemosensory organs of a herbivorous insect, for which chemosensation guides most of its host preferences. A European and an African population of the noctuid Sesamia nonagrioides that display significant differences in their ecological preferences were collected on Zea mays and Typha domingensis, respectively. RNAseq were used between the two populations for digital expression profiling of chemosensory organs from larval antennae and palps. Preliminary data on adult female antennae and ovipositors were also collected. We found 6,550 differentially expressed transcripts in larval antennae and palps. Gene ontology enrichment analyses suggested that transcriptional activity was overrepresented in the French population and that virus and defense activities were overrepresented in the Kenyan population. In addition, we found differential expression of a variety of cytochrome P450s, which may be linked to the different host-plant diets. Looking at olfactory genes, we observed differential expression of numerous candidate odorant-binding proteins, chemosensory proteins, and one olfactory receptor, suggesting that differences in olfactory sensitivity participate in insect adaptation.
authors: INRA, UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris, Route de Saint-Cyr, F-78026 Versailles Cedex, France; UMR Evolution, Génomes, Comportement et Ecologie, IRD, CNRS, Université Paris Sud, Campus CNRS, 91198 Gif-sur-Yvette Cedex, France.
authors: Nicolas
authors: N
authors: Glaser
authors: INRA, UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris, Route de Saint-Cyr, F-78026 Versailles Cedex, France; IRISA, équipe GenScale, Campus universitaire de Beaulieu, 35042 Rennes Cedex, France.
authors: Aurore
authors: A
authors: Gallot
authors: IRISA, équipe GenScale, Campus universitaire de Beaulieu, 35042 Rennes Cedex, France.
authors: Fabrice
authors: F
authors: Legeai
authors: UMR Evolution, Génomes, Comportement et Ecologie, IRD, CNRS, Université Paris Sud, Campus CNRS, 91198 Gif-sur-Yvette Cedex, France; Université Paris-Sud 11, 91405 Orsay Cedex, France.
authors: Myriam
authors: M
authors: Harry
authors: UMR Evolution, Génomes, Comportement et Ecologie, IRD, CNRS, Université Paris Sud, Campus CNRS, 91198 Gif-sur-Yvette Cedex, France.
authors: Laure
authors: L
authors: Kaiser
authors: UMR Evolution, Génomes, Comportement et Ecologie, IRD, CNRS, Université Paris Sud, Campus CNRS, 91198 Gif-sur-Yvette Cedex, France; UMR Evolution, Génomes, Comportement et Ecologie IRD, CNRS, Université Paris Sud, c/o icipe, NSBB Project, PO Box 30772-00100, Nairobi, Kenya.
authors: Bruno
authors: B
authors: Le Ru
authors: UMR Evolution, Génomes, Comportement et Ecologie, IRD, CNRS, Université Paris Sud, Campus CNRS, 91198 Gif-sur-Yvette Cedex, France; UMR Evolution, Génomes, Comportement et Ecologie IRD, CNRS, Université Paris Sud, c/o icipe, NSBB Project, PO Box 30772-00100, Nairobi, Kenya.
authors: Paul-André
authors: PA
authors: Calatayud
authors: INRA, UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris, Route de Saint-Cyr, F-78026 Versailles Cedex, France. Electronic address: emmanuelle.jacquin@versailles.inra.fr.
authors: Emmanuelle
authors: E
authors: Jacquin-Joly
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D005260:Female; D005602:France; D020869:Gene Expression Profiling; D017344:Genes, Insect; D007630:Kenya; D007814:Larva / Q000235:genetics / Q000378:metabolism; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D010058:Oviposition; D012903:Smell; D059467:Transcriptome; D032401:Typhaceae / Q000469:parasitology; D003313:Zea mays / Q000469:parasitology
chemical_list: D003577:Cytochrome P-450 Enzyme System
keywords: Adaptation; Chemosensory genes; RNAseq; Sesamia nonagrioides