title: Luteipulveratus halotolerans sp. nov., an actinobacterium (Dermacoccaceae) from forest soil.
abstract: The taxonomic position of an actinobacterium strain, C296001T, isolated from a soil sample collected in Sarawak, Malaysia, was established using a polyphasic approach. Phylogenetically, strain C296001T was closely associated with the genus Luteipulveratus and formed a distinct monophyletic clade with the only described species, Luteipulveratus mongoliensis NBRC 105296T. The 16S rRNA gene sequence similarity between strain C296001T and L. mongoliensis was 98.7 %. DNA-DNA hybridization results showed that the relatedness of strain C296001T to L. mongoliensis was only 21.5 %. The DNA G+C content of strain C296001T was 71.7 mol%. Using a PacBio RS II system, whole genome sequences for strains C296001T and NBRC 105296T were obtained. The genome sizes of 4.5 Mbp and 5.4 Mbp determined were similar to those of other members of the family Dermacoccaceae. The cell-wall peptidoglycan contained lysine, alanine, aspartic acid, glutamic acid and serine, representing the peptidoglycan type A4α l-Lys-l-Ser-d-Asp. The major menaquinones were MK-8(H4), MK-8 and MK-8(H2). Phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and phosphoglycolipid were the polar lipids, while the whole-cell sugars were glucose, fucose and lesser amounts of ribose and galactose. The major fatty acids were iso-C16 : 0, anteiso-C17 : 0, iso-C16 : 1 H, anteiso-C17 : 1ω9c, iso-C18 : 0 and 10-methyl C17 : 0. Chemotaxonomic analyses showed that C296001T had typical characteristics of members of the genus Luteipulveratus, with the main differences occurring in phenotypic characteristics. On the basis of the phenotypic and chemotaxonomic evidence, it is proposed that strain C296001T be classified as a representative of a novel species in the genus Luteipulveratus, for which the name Luteipulveratus halotolerans sp. nov. is recommended. The type strain is C296001T ( = ATCC TSD-4T = JCM 30660T).
authors: Sarawak Biodiversity Centre, Km. 20 Jalan Borneo Heights, Semengoh, 93250 Kuching, Sarawak, Malaysia.
authors: Holed
authors: H
authors: Juboi
authors: Sarawak Biodiversity Centre, Km. 20 Jalan Borneo Heights, Semengoh, 93250 Kuching, Sarawak, Malaysia.
authors: Ann Anni
authors: AA
authors: Basik
authors: Sarawak Biodiversity Centre, Km. 20 Jalan Borneo Heights, Semengoh, 93250 Kuching, Sarawak, Malaysia.
authors: Sunita Sara Gill
authors: SSG
authors: Shamsul
authors: Novartis Institutes for BioMedical Research, Novartis Campus, CH-4058 Basel, Switzerland.
authors: Phil
authors: P
authors: Arnold
authors: Novartis Institutes for BioMedical Research, Novartis Campus, CH-4058 Basel, Switzerland.
authors: Esther K
authors: EK
authors: Schmitt
authors: Sarawak Biodiversity Centre, Km. 20 Jalan Borneo Heights, Semengoh, 93250 Kuching, Sarawak, Malaysia.
authors: Jean-Jacques
authors: JJ
authors: Sanglier
authors: Sarawak Biodiversity Centre, Km. 20 Jalan Borneo Heights, Semengoh, 93250 Kuching, Sarawak, Malaysia.
authors: Tiong Chia
authors: TC
authors: Yeo
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D065928:Forests; D008296:Malaysia; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8