title: Neo-epitopes on crotonaldehyde modified DNA preferably recognize circulating autoantibodies in cancer patients.
abstract: DNA damage is one of the leading causes of various pathological conditions including carcinogenesis. Crotonaldehyde is a 4-carbon unsaturated bifunctional aldehyde which is found ubiquitously and produced both exogenously and endogenously. It reacts with deoxyguanosine and form adducts with DNA. These adducts were detected and found involved in tumor formation in rats treated with crotonaldehyde. In the present study, structural changes in DNA by crotonaldehyde were evaluated by Fourier transform infrared (FTIR) spectroscopy, differential scanning colorimetry (DSC), dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), and atomic force microscopy (AFM). Enhanced binding was observed in cancer autoantibodies with the DNA modified by crotonaldehyde than the native counterpart. Immunological studies revealed enhanced binding of cancer autoantibodies with crotonaldehyde modified DNA, compared to the native form. Furthermore, lymphocyte DNA isolated from cancer patients demonstrated considerable recognition of anti-Cro-DNA IgG as compared to the DNA from healthy individuals. Therefore, we suggest that crotonaldehyde modified DNA presents unique epitopes, that may trigger autoantibody induction in cancer patients.
authors: Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
authors: Badar
authors: B
authors: Ul Islam
authors: Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
authors: Parvez
authors: P
authors: Ahmad
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, UP, India.
authors: Gulam
authors: G
authors: Rabbani
authors: Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
authors: Kiran
authors: K
authors: Dixit
authors: Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
authors: Moinuddin
authors: Department of Radiotherapy, J. N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
authors: Shahid Ali
authors: SA
authors: Siddiqui
authors: Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India. asifali_amu@rediffmail.com.
authors: Asif
authors: A
authors: Ali
mesh_terms: D000328:Adult; D000447:Aldehydes / Q000627:therapeutic use*; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D004247:DNA / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000276:immunology
chemical_list: D000447:Aldehydes; D001323:Autoantibodies; D000939:Epitopes; D007074:Immunoglobulin G; D004247:DNA; C012796:2-butenal
keywords: Cancer; Crosslinking; Crotonaldehyde; Human DNA; Neo-epitopes