title: Ya-fish (Schizothorax prenanti) spexin: identification, tissue distribution and mRNA expression responses to periprandial and fasting.
abstract: Spexin (SPX) is a novel peptide which was known for its role in physiological homeostasis. A recent study has confirmed that SPX plays an important role in the feeding regulation. However, the reports about SPX are very limited. In the present study, we characterized the structure, distribution and mRNA expression responses to feeding status of SPX in Ya-fish (Schizothorax prenanti). The full-length cDNA of Ya-fish SPX was 1330 base pairs (bp), which encoded 106 amino acid residues. These residues contained a 31-amino acid signal peptide region and a 14-amino acid mature peptide. The sequence alignment demonstrated that the Ya-fish SPX showed high conservation with other species. Our data revealed that SPX was widely expressed in all test tissues. The highest expression of SPX mRNA was observed in Ya-fish forebrain. Compared with the Ya-fish SPX mRNA expression in the forebrain between the preprandial and postprandial groups, the fed group was prominently increased than unfed groups after a meal, while the unfed group at 1 and 3 h substantially decreased than preprandial groups (P < 0.01). In addition, SPX mRNA expression in forebrain was significantly decreased (P < 0.01) during fasting for a week and sharply increased (P < 0.01) after refeeding on the 7th day, and then return to normal level on the 9th day. These results point toward that SPX mRNA expression is regulated by metabolic status or feeding conditions in Ya-fish.
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.|Chengdu Agricultural College, 392# Detong Bridge, Chengdu, China.
authors: Hongwei
authors: H
authors: Wu
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Fangjun
authors: F
authors: Lin
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Hu
authors: H
authors: Chen
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Ju
authors: J
authors: Liu
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Yundi
authors: Y
authors: Gao
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Xin
authors: X
authors: Zhang
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Jin
authors: J
authors: Hao
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Defang
authors: D
authors: Chen
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Dengyue
authors: D
authors: Yuan
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
authors: Tao
authors: T
authors: Wang
authors: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China. lizhiqiong454@163.com.
authors: Zhiqiong
authors: http://orcid.org/0000-0001-8880-2240
authors: Z
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005215:Fasting / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D036361:Peptide Hormones / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D019518:Postprandial Period / Q000502:physiology*; D016548:Prosencephalon / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D036361:Peptide Hormones; D012333:RNA, Messenger
keywords: Cloning; Fasting; Refeeding; Structure; Tissue distribution; Ya-fish