title: Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation.
  
  
  RESULTS
  We identified 29 clones containing 40 microsatellites from a genomic enriched library. A total of 27 primer pairs were developed and evaluated in 30 individuals of a natural P. minensis population. Seventeen markers successfully presented amplification products within the expected size range, of which 12 were polymorphic. The expected and observed heterozygosities ranged from 0.03 to 0.65 and from 0.00 to 0.77, respectively. Positive transferability with the related species P. bahiensis was observed for the same 17 markers.
  
  
  CONCLUSIONS
  The 12 polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene flow in P. minensis and also may be useful for similar issues regarding the related species P. bahiensis.
authors: Departamento de Biologia Vegetal, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors: André V
authors: AV
authors: Scatigna
authors: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors: Fernanda A
authors: FA
authors: Oliveira
authors: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors: Camila C
authors: CC
authors: Mantello
authors: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors: Patrícia M
authors: PM
authors: Francisco
authors: Departamento de Biologia Vegetal, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil ; Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors: Anete P
authors: AP
authors: Souza
authors: Departamento de Biologia Vegetal, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
authors: André O
authors: AO
authors: Simões
keywords: Espinhaço Range; Gratioleae; Philcoxia minensis; Plantaginaceae; conservation genetics; simple sequence repeat (SSR)