title: AFM single-cell force spectroscopy links altered nuclear and cytoskeletal mechanics to defective cell adhesion in cardiac myocytes with a nuclear lamin mutation.
abstract: Previous investigations suggested that lamin A/C gene (LMNA) mutations, which cause a variety of human diseases including muscular dystrophies and cardiomyopathies, alter the nuclear mechanical properties. We hypothesized that biomechanical changes may extend beyond the nucleus.
authors: a Department of Engineering and Architecture ; University of Trieste ; Trieste Italy.
authors: Thomas
authors: T
authors: Lanzicher
authors: a Department of Engineering and Architecture ; University of Trieste ; Trieste Italy.|b International Center for Genetic Engineering and Biotechnology ; Trieste Italy.
authors: Valentina
authors: V
authors: Martinelli
authors: c Cardiovascular Institute & Adult Medical Genetics; University of Colorado Denver Anschutz Medical Campus ; CO USA.
authors: Carlin S
authors: CS
authors: Long
authors: d Department of Food Chemistry and Toxicology ; University of Vienna ; Waehringer Str. 38A-1090 Vienna Austria.
authors: Giorgia
authors: G
authors: Del Favero
authors: a Department of Engineering and Architecture ; University of Trieste ; Trieste Italy.
authors: Luca
authors: L
authors: Puzzi
authors: e Department of Life Sciences ; University of Trieste ; Trieste Italy.
authors: Massimo
authors: M
authors: Borelli
authors: c Cardiovascular Institute & Adult Medical Genetics; University of Colorado Denver Anschutz Medical Campus ; CO USA.
authors: Luisa
authors: L
authors: Mestroni
authors: c Cardiovascular Institute & Adult Medical Genetics; University of Colorado Denver Anschutz Medical Campus ; CO USA.
authors: Matthew R G
authors: MR
authors: Taylor
authors: a Department of Engineering and Architecture ; University of Trieste ; Trieste Italy.|c Cardiovascular Institute & Adult Medical Genetics; University of Colorado Denver Anschutz Medical Campus ; CO USA.
authors: Orfeo
authors: O
authors: Sbaizero
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002467:Cell Nucleus / Q000378:metabolism*; D048430:Cell Shape; D003599:Cytoskeleton / Q000378:metabolism*; D055119:Elastic Modulus; D006801:Humans; D034904:Lamin Type A / Q000235:genetics*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008954:Models, Biological; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D017208:Rats, Wistar; D013057:Spectrum Analysis / Q000379:methods*; D014404:Tubulin / Q000378:metabolism; D014783:Viscosity
chemical_list: D034904:Lamin Type A; D050505:Mutant Proteins; D014404:Tubulin
keywords: AFM; Young Modulus; cardiomyocytes; cardiomyopathy; cell physiology; lamin A/C; relaxation force test