title: [Determination of 11 sulfonamide residues in aquaculture water and sediments by high performance liquid chromatography coupled with post-column derivatization].
abstract: An analytical method was developed for the determination of 11 sulfonamide compounds in aquaculture water and sediments by high performance liquid chromatography (HPLC) coupled with post-column derivatization. The filtered water sample was purified and concentrated with HLB cartridge, while the sediment sample was extracted with a mixture of methanol and EDTA-McIlvaine buffer (1:1, v/v), and then purified and enriched through HLB solid-phase extraction. The sulfonamides were separated on a C18 column by HPLC and on-line derivatized with a fluorescamine and detected with a fluorescence detector. The parameters of post-column derivatization system were optimized, and the fluorescamine solution concentration, velocity of reagent solution and reaction temperature were 0.2 g/L, 0.15 mL/min and 50 °C, respectively. The calibration curves of the method showed good linearity in the range of 0.01-1.0 mg/L, with the correlation coefficients (r2) all above 0.99995. The recoveries were 79.3%-100.7% and 74.6%-95.3% with RSD values of 2.2%-11.0% and 2.6%-10.3% for the 11 sulfonamides in aquaculture water and sediments, respectively. The respective limits of detection (LODs, S/N = 3) were 0.9-5.5 ng/L and 0.3-1.3 µg/kg and the limits of quantification (LOQs, S/N = 10) were 3.0-18.1 ng/L and 1.0-4.4 µg/kg. The method can be applied to the determination of sulfonamides in the aquaculture environment, and it has a good practicability.
authors: Jinghua
authors: J
authors: Liu
authors: Zhenzhong
authors: Z
authors: Sun
authors: Xueling
authors: X
authors: Huang
authors: Xia
authors: X
authors: Guo
authors: Jianhua
authors: J
authors: Sun
mesh_terms: D017756:Aquaculture; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D019015:Geologic Sediments / Q000032:analysis*; D057230:Limit of Detection; D052616:Solid Phase Extraction; D013449:Sulfonamides / Q000032:analysis*; D014867:Water / Q000032:analysis*
chemical_list: D013449:Sulfonamides; D014867:Water