title: Highly Sensitive Electrochemical Determination of Alfatoxin B1 Using Quantum Dots-Assembled Amplification Labels.
abstract: A competitive electrochemical immunoassay for highly sensitive detection of AFB1 is demonstrated using layer-by-layer (LBL) assembled quantum dots (QDs) as labels. To investigate the effects of the higher sensitivity of square wave voltammetric stripping (SWV) and of the LBL technique on the proposed immunoassays, the proposed assay was compared to electrochemical (EC) and fluorescent immunoassays, which did not use LBL technology. Peanut samples were analyzed using the three immunoassays. The limits of detection (LODs) were 0.018, 0.046 and 0.212 ng/mL, respectively, while the sensitivities were 0.308, 1.011 and 4.594 ng/mL, respectively. The proposed electrochemical immunoassay displayed a significant improvement in sensitivity, thereby providing a simple and sensitive alternative strategy for determining AFB1 levels in peanut samples.
authors: Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China. zengxiaoqun@nbu.edu.cn.
authors: Xiaoqun
authors: X
authors: Zeng
authors: Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China. huijugao@163.com.
authors: Huiju
authors: H
authors: Gao
authors: Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China. daodongpan@163.com.|Food Science & Nutrition Department, Nanjing Normal University, Nanjing 210097, China. daodongpan@163.com.
authors: Daodong
authors: D
authors: Pan
authors: Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China. sunyangying@nbu.edu.cn.
authors: Yangying
authors: Y
authors: Sun
authors: Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China. caojinxuan@nbu.edu.cn.
authors: Jinxuan
authors: J
authors: Cao
authors: Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China. woodsen@163.com.
authors: Zhen
authors: Z
authors: Wu
authors: School of Electronic Engineering, Xidian University, Xi'an 710071, China. msnagisap@gmail.com.
authors: Zhenyu
authors: Z
authors: Pan
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D010367:Arachis / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005453:Fluorescence; D007118:Immunoassay; D007854:Lead / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012995:Solubility; D013440:Sulfides / Q000737:chemistry; D013864:Thioglycolates / Q000737:chemistry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D013440:Sulfides; D013864:Thioglycolates; D014867:Water; C018391:lead sulfide; D007854:Lead; C017487:2-mercaptoacetate; D016604:Aflatoxin B1
keywords: AFB1 detection; electrochemical immunoassay; layer-by-layer assembly; quantum dots