title: Integrated multi-omics analysis supports role of lysophosphatidylcholine and related glycerophospholipids in the Lotus japonicus-Glomus intraradices mycorrhizal symbiosis.
abstract: Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus, we performed an integrated multi-omics analysis with a focus on plant and fungal phospholipid (PL) metabolism and biological significance of lysophosphatidylcholine (LPC). Our results support the role of LPC as a bioactive compound eliciting cellular and molecular response mechanisms in Lotus. Evidence is provided for large interspecific chemical diversity of LPC species among mycorrhizae with related AMF species. Lipid, gene expression and elemental profiling emphasize the Lotus-Glomus intraradices interaction as distinct from other arbuscular mycorrhizal (AM) interactions. In G. intraradices, genes involved in fatty acid (FA) elongation and biosynthesis of unsaturated FAs were enhanced, while in Lotus, FA synthesis genes were up-regulated during AMS. Furthermore, FAS protein localization to mitochondria suggests FA biosynthesis and elongation may also occur in AMF. Our results suggest the existence of interspecific partitioning of PL resources for generation of LPC and novel candidate bioactive PLs in the Lotus-G. intraradices symbiosis. Moreover, the data advocate research with phylogenetically diverse Glomeromycota species for a broader understanding of the molecular underpinnings of AMS.
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.|Department of Plant Pathology, The Ohio State University, Kottman Hall, 2021 Coffey Road, Columbus, OH, 43210, USA.
authors: Vinod
authors: V
authors: Vijayakumar
authors: Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053, Regensburg, Germany.
authors: Gerhard
authors: G
authors: Liebisch
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.|Bayer CropScience AG, Alfred-Nobel-Straße 50, D-40789, Monheim am Rhein, Germany.
authors: Benjamin
authors: B
authors: Buer
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.
authors: Li
authors: L
authors: Xue
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.
authors: Nina
authors: N
authors: Gerlach
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.
authors: Samira
authors: S
authors: Blau
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.|Plant Molecular Physiology and Biotechnology, Heinrich Heine University, D-40225, Düsseldorf, Germany.
authors: Jessica
authors: J
authors: Schmitz
authors: Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.
authors: Marcel
authors: M
authors: Bucher
mesh_terms: D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055137:Glomeromycota / Q000187:drug effects / Q000502:physiology*; D020404:Glycerophospholipids / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D000070116:Lotus / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D008954:Models, Biological; D038821:Mycorrhizae / Q000187:drug effects / Q000502:physiology*; D010710:Phosphates / Q000494:pharmacology; D040901:Proteomics / Q000379:methods*; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013347:Subcellular Fractions / Q000378:metabolism; D013559:Symbiosis / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
chemical_list: D020404:Glycerophospholipids; D008244:Lysophosphatidylcholines; D010710:Phosphates; D012333:RNA, Messenger
keywords: Glomeromycota; Glomus intraradices (syn: Rhizophagus irregularis); arbuscular mycorrhiza; fatty acid synthesis; ionomics; lipidomics; phospholipids; transcriptomics