title: CD99-like 2 (CD99L2)-deficient mice are defective in the acute inflammatory response.
abstract: CD99-Like 2 (CD99L2) is a Type I glycoprotein expressed on leukocytes and endothelial cells as well as other cell types. It is related to CD99, although it shows only 38% sequence identity. CD99L2 has been shown to play a role in leukocyte extravasation in mice under various inflammatory conditions using anti-CD99L2 antibodies and, in one case by targeted deletion of CD99L2. We report here studies on an independently made CD99L2 "knockout mouse" that extend our knowledge of the role of CD99L2 in inflammation. CD99L2 deficiency did not affect the total or relative numbers of circulating leukocyte subsets, red blood cells, or platelets. Neither did CD99L2 deficiency affect the expression of ICAM-1, PECAM, or CD99 on endothelial cells. Mice lacking CD99L2 had a defective inflammatory response in the thioglycollate peritonitis model with a greater than 80% block in neutrophil infiltration and a nearly complete block in monocyte emigration into the peritoneal cavity measured 16h after the inflammatory challenge. The mice will be a useful resource to study the role of CD99L2 in various acute and chronic inflammatory diseases.
authors: Department of Pathology,Northwestern University Feinberg School of Medicine,303 East Chicago Avenue,Chicago,IL 60611, United States.
authors: Nakisha S
authors: NS
authors: Rutledge
authors: Department of Pathology,Northwestern University Feinberg School of Medicine,303 East Chicago Avenue,Chicago,IL 60611, United States.
authors: Evan W
authors: EW
authors: Weber
authors: Department of Pathology,Northwestern University Feinberg School of Medicine,303 East Chicago Avenue,Chicago,IL 60611, United States.
authors: Ryan
authors: R
authors: Winger
authors: Department of Pathology,Northwestern University Feinberg School of Medicine,303 East Chicago Avenue,Chicago,IL 60611, United States; Department of Neurology,Northwestern University Feinberg School of Medicine,303 East Chicago Avenue,Chicago,IL 60611, United States.
authors: Warren G
authors: WG
authors: Tourtellotte
authors: Transplantation Research Institute,MRC,Seoul National University College of Medicine Seoul,South Korea.
authors: Seong Hoe
authors: SH
authors: Park
authors: Department of Pathology,Northwestern University Feinberg School of Medicine,303 East Chicago Avenue,Chicago,IL 60611, United States. Electronic address: wamuller@northwestern.edu.
authors: William A
authors: WA
authors: Muller
mesh_terms: D000071443:12E7 Antigen; D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002465:Cell Movement; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D007249:Inflammation / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007962:Leukocytes; D018345:Mice, Knockout; D009504:Neutrophils / Q000473:pathology
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; C522605:Cd99 protein, mouse; D018799:Intercellular Adhesion Molecule-1
keywords: Adhesion molecule; CD99L2; Endothelium; Inflammation; Knockout mouse; Leukocyte