title: The potential anti-proliferative effect of β-sitosterol on human mast cell line-1 cells.
abstract: Thymic stromal lymphopoietin (TSLP) was reported to induce mast cell proliferation and aggravate allergic reactions through activation of mouse double minute 2 (MDM2). We aimed to ascertain that β-sitosterol (SI), which is one of the several phytosterols found mostly in foods, would regulate TSLP-induced mast cell proliferation. The results showed that SI significantly decreased the proliferation of human mast cell line (HMC-1) cells promoted by TSLP. SI significantly decreased the mRNA expression of Ki-67 in the TSLP-treated HMC-1 cells. SI significantly suppressed the production and mRNA expression of interleukin-13 in the TSLP-treated HMC-1 cells. Furthermore, SI downregulated the expression of MDM2 and phosphorylation of STAT6, whereas it upregulated the expression of p53, activation of caspase-3, and cleavage of poly ADP-ribose polymerase in the TSLP-treated HMC-1 cells. Results of this study suggest that SI may be a potential therapeutic agent for mast cell-mediated allergic diseases.
authors: a Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors: Na-Ra
authors: NR
authors: Han
authors: a Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors: Hyung-Min
authors: HM
authors: Kim
authors: b Department of Food Technology and Inflammatory Disease Research Center, Hoseo University, 20, Hoseo-ro 79beon-gil, Baebang-eup, Asan 336-795, Republic of Korea.
authors: Hyun-Ja
authors: HJ
authors: Jeong
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018793:Interleukin-13 / Q000037:antagonists & inhibitors / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D012855:Sitosterols / Q000494:pharmacology*
chemical_list: C578095:IL13 protein, human; D018793:Interleukin-13; D012855:Sitosterols; C025473:gamma-sitosterol
keywords: lymphopoïétine stromale thymique; mast cells; mastocytes; mouse double minute 2; proliferation; prolifération; thymic stromal lymphopoietin; β-sitosterol; β-sitostérol