title: The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice.
abstract: Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atopic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.
authors: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.|Division of Allergy and Chronic Respiratory Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 361-951, Korea.
authors: Hye-Sun
authors: HS
authors: Lim
authors: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors: Hyekyung
authors: H
authors: Ha
authors: K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors: Hyeun-Kyoo
authors: HK
authors: Shin
authors: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.|Korea Medicine Life Science, University of Science & Technology, Daejeon 305-350, Korea.
authors: Soo-Jin
authors: SJ
authors: Jeong
mesh_terms: D000818:Animals; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D063990:Gene Ontology; D016678:Genome; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D039981:Pyroglyphidae / Q000276:immunology; D030441:Saussurea / Q000737:chemistry; D015398:Signal Transduction; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts
keywords: Nc/Nga mouse model; PI3Kca; Saussurea lappa; atopic dermatitis; microarray