title: Evidence for the contribution of BDNF-TrkB signal strength in neurogenesis: An organotypic study.
abstract: The importance of neurotrophins, especially brain-derived neurotrophic factor (BDNF) in the regulation of mammalian neurogenesis has been extensively studied. However, the exact effects of BDNF on cell proliferation and neurogenesis remain controversial. Here we tried to use an organotypic hippocampal slice culture (OHSC) to precisely control the concentration of BDNF and investigate their effects on neuro- and gliogenesis in vitro. With chronic supplementation of various concentration of the recombinant BDNF in culture medium, the number of newly born neurons was highly increased in a concentration dependent manner, while the number of glial cells remained unchanged. Blocking TrkB-BDNF signal pathway led to inhibition of neurogenesis. Time series analysis of BDNF and TrkB expression revealed relative low levels of BDNF and TrkB expression during early culture period, which might account for the results that early BDNF supplementation was more effective in the promotion of neurogenesis than late supplementation. These observations suggested that appropriate development-related modulation of BDNF-TrkB signal strength might impact on the initiation and maintenance of neurogenesis.
authors: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China; Department of Neurology, Liwan Hospital of Guangzhou Medical University, Guangzhou, China.
authors: Zhaoxia
authors: Z
authors: Wei
authors: Surgery Department of Longhua New District People's Hospital of Shenzhen, Shenzhen, China.
authors: Jiacheng
authors: J
authors: Liao
authors: Department of Neurology, Liwan Hospital of Guangzhou Medical University, Guangzhou, China.
authors: Feng
authors: F
authors: Qi
authors: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, China.
authors: Zhao
authors: Z
authors: Meng
authors: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address: sypan_sum@sina.com.
authors: Suyue
authors: S
authors: Pan
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D002452:Cell Count; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D020813:Receptor, trkB
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D008562:Membrane Glycoproteins; D011505:Protein-Tyrosine Kinases; C064371:Ntrk2 protein, mouse; D020813:Receptor, trkB
keywords: BDNF; Neurogenesis; Organotypic hippocampal slice culture; TrkB