title: Energy and antioxidant responses of pacific oyster exposed to trace levels of pesticides.
abstract: Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 μg/L), isoproturon (0.1 and 1 μg/L), or both in a mixture (0.2 and 0.1 μg/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase α (AMPKα), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was up-regulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters.
authors: Ifremer, UMR 6539 (CNRS, UBO, IRD, Ifremer), Laboratoire des sciences de l'Environnement Marin (LEMAR), 29280 Plouzané, France.
authors: Yanouk
authors: Y
authors: Epelboin
authors: Ifremer, UMR 6539 (CNRS, UBO, IRD, Ifremer), Laboratoire des sciences de l'Environnement Marin (LEMAR), 29280 Plouzané, France.
authors: Claudie
authors: C
authors: Quéré
authors: Ifremer, UMR 6539 (CNRS, UBO, IRD, Ifremer), Laboratoire des sciences de l'Environnement Marin (LEMAR), 29280 Plouzané, France.
authors: Fabrice
authors: F
authors: Pernet
authors: UBO, UMR 6539 (CNRS, UBO, IRD, Ifremer), Laboratoire des sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), Université de Brest , 29280 Plouzané, France.
authors: Vianney
authors: V
authors: Pichereau
authors: Ifremer, UMR 6539 (CNRS, UBO, IRD, Ifremer), Laboratoire des sciences de l'Environnement Marin (LEMAR), 29280 Plouzané, France.
authors: Charlotte
authors: C
authors: Corporeau
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D010125:Ostreidae / Q000187:drug effects* / Q000378:metabolism; D010575:Pesticides / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D010575:Pesticides; D014874:Water Pollutants, Chemical; D000263:Adenylate Kinase