title: Distinctive expression pattern of interleukin-17 cytokine family members in colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common cancers in both genders. Even though interleukin (IL)-17A was shown to play an important role in intestinal tumourigenesis and CRC, other IL-17 family members were not studied well. We therefore studied the expression of IL-17 cytokine family members in CRC. Ten healthy colons and ten CRC mucosa were immunostained for IL-17B, IL-17C, IL-17E, and IL-17F, and their receptors IL-17RA, IL-17RB, and IL-17RC. Double immunofluorescence staining of the CRC mucosa was done for IL-17B with markers of neutrophils, endothelial cells, macrophages, T cells, mast cells, or fibroblasts. While IL-17B was increased in CRC with a strong presence both in the epithelial and stromal compartments, IL-17C showed different expression depending on the grade of differentiation and IL-17E remained unchanged. In contrast, IL-17F was decreased in CRC compared to healthy control. Colon epithelial cells stained positive for IL-17RA, IL-17RB, and IL-17RC in both healthy control and CRC. Neutrophils were the main source of IL-17B in the stroma. IL-17 family members demonstrated distinct expression patterns in CRC, suggesting a differential role exerted by each member in colon carcinogenesis.
authors: Department of Medicine, Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.|Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
authors: Ahmed
authors: A
authors: Al-Samadi
authors: Digestive Oncology Research Center; Digestive Disease Research Institute, Tehran University of Medical Sciences, Shariati Hospital, North Amirabad Ave., Tehran, 14117, Iran. shirin.moossavi@gmail.com.
authors: Shirin
authors: S
authors: Moossavi
authors: Department of Medicine, Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.
authors: Abdelhakim
authors: A
authors: Salem
authors: Pathology Department, Tehran University of Medical Sciences, Tehran, Iran.
authors: Masoud
authors: M
authors: Sotoudeh
authors: Department of Medicine, Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.
authors: Sarianna M
authors: SM
authors: Tuovinen
authors: Department of Medicine, Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.|Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland.|ORTON Orthopedic Hospital of the Invalid Foundation, Helsinki, Finland.
authors: Yrjö T
authors: YT
authors: Konttinen
authors: Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
authors: Tuula
authors: T
authors: Salo
authors: Division of Digestive Diseases, Rush University Medical Center, Chicago, IL, USA.
authors: Faraz
authors: F
authors: Bishehsari
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000378:metabolism
chemical_list: D020381:Interleukin-17
keywords: Colorectal cancer; IL-17; IL-17B; Neutrophil