title: Adhesion and biofilm formation in artificial saliva and susceptibility of yeasts isolated from chronic kidney patients undergoing haemodialysis.
abstract: Yeasts of the genera Candida and Saccharomyces are opportunist pathogens and cause oral lesions, especially in immunocompromised patients. This study assessed yeasts isolated from chronic kidney patients undergoing haemodialysis for their adhesion capacity, biofilm formation and susceptibility to antifungal agents. Ten isolates of Candida spp. and one isolate of Saccharomyces cerevisiae were tested for adhesion to buccal epithelial cells (BECs), adhesion and formation of biofilm in artificial saliva and their susceptibility profile to antifungal agents. Adhesion and biofilm formation were undertaken in polystyrene plates with artificial saliva, whilst susceptibility to antifungal agents was evaluated by broth microdilution. Candida parapsilosis had the highest adhesion index in BECs (154.55 ± 22.13) and Candida rugosa was the species with the highest adhesion capacity (18 398  Abs cm(-2)) in abiotic surface with artificial saliva. Candida albicans provided the greatest biofilm formation (2035  Abs cm(-2) ± 0.09) but was revealed to be susceptible to the five antifungal agents under analysis. However, some non-albicans Candida isolates showed a lower susceptibility for the antifungal agents itraconazole, fluconazole and voriconazole. All of the species were sensitive to amphotericin B and nystatin. The current analysis showed that yeasts isolated from the mouth of chronic kidney patients undergoing haemodialysis varied significantly with regard to their capacity for adherence, biofilm formation and susceptibility to antifungal agents, underscoring the high virulence of non-albicans Candida species.
authors: Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
authors: Paula Assis
authors: PA
authors: Queiroz
authors: Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
authors: Janine Silva Ribeiro
authors: JSR
authors: Godoy
authors: Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
authors: Patrícia de Souza Bonfim
authors: PSB
authors: Mendonça
authors: Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
authors: Raíssa Bocchi
authors: RB
authors: Pedroso
authors: Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
authors: Terezinha Inez Estivalet
authors: TIE
authors: Svidzinski
authors: Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
authors: Melyssa
authors: M
authors: Negri
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000502:physiology*; D002448:Cell Adhesion; D006801:Humans; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D012464:Saliva, Artificial / Q000737:chemistry*
chemical_list: D012464:Saliva, Artificial