title: Serological and molecular survey of sheep infected with Mycoplasma ovipneumoniae in Xinjiang, China.
abstract: Mycoplasma pneumonia is one of the most important infectious diseases that threaten sheep production. In order to investigate the epidemic status of Mycoplasma ovipneumoniae infection in sheep, indirect hemagglutination assay was used to analyze 1679 serum samples collected from four different breeds of sheep (Kazak sheep, Hu sheep, Merino sheep, and Duolang sheep) in six regions in Xinjiang between 2012 and 2014. One thousand one hundred sixty-nine sheep nasal swabs and 180 lungs were PCR analyzed. The results showed that the average positive rates of the serum samples were 17.75 %. The positive rates were between 9.76 and 30.61 % in the four breeds. Among them, the Hu sheep had a significantly higher rate than other breeds (P < 0.05). The average positive rates of nasal swabs and lungs were 10.18 and 28.89 %, respectively. Based on the phylogenetic trees of 16S RNA gene, the isolates were closest to those strains isolated from inland areas of China, indicating that these epidemic isolates came from the trans-province introductions. Our survey suggests that quarantine is necessary for sheep imported from inland, and effective immunization should be implemented in sheep susceptible to M. ovipneumoniae in Xinjiang, China.
authors: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors: Chen
authors: C
authors: Cheng
authors: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China. qj710625@163.com.
authors: Qiao
authors: Q
authors: Jun
authors: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors: Meng
authors: M
authors: Qingling
authors: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors: Hu
authors: H
authors: Zhengxiang
authors: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors: Ma
authors: M
authors: Yu
authors: State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.
authors: Cai
authors: C
authors: Xuepeng
authors: Center for Animal Disease Prevention and Control, Tacheng, 834700, Xinjiang, China.
authors: Cheng
authors: C
authors: Zibing
authors: Center for Animal Disease Prevention and Control, Tacheng, 834700, Xinjiang, China.
authors: Zhang
authors: Z
authors: Jinsheng
authors: Center for Animal Disease Prevention and Control, Changji, 831100, Xinjiang, China.
authors: Zhang
authors: Z
authors: Zaichao
authors: Center for Animal Disease Prevention and Control, Xinjiang, 830000, China.
authors: Cai
authors: C
authors: Kuojun
authors: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
authors: Chen
authors: C
authors: Chuangfu
mesh_terms: D000818:Animals; D002681:China; D006386:Hemagglutination Tests; D008168:Lung; D045802:Mycoplasma ovipneumoniae / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012756:Sheep / Q000235:genetics; D012757:Sheep Diseases / Q000453:epidemiology*; D034561:Sheep, Domestic / Q000382:microbiology; D011795:Surveys and Questionnaires
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: China; Mycoplasma ovipneumoniae; Serological and molecular survey; Sheep; Xinjiang