title: A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress.
abstract: Vitamin K epoxide reductase (VKOR) exists widely in the chloroplasts of higher plants and plays important roles in redox regulation. However, investigations of plant VKOR function have primarily focused on VKOR from Arabidopsis, and knowledge concerning this function is therefore quite limited. In this study, Solanum lycopersicum was used to study the function of VKOR. Transcription level analysis revealed that SlVKOR (S. lycopersicum VKOR) expression was upregulated by salt or drought stress. To further investigate the function of SlVKOR in plants, we generated sense and antisense transgenic S. lycopersicum homozygotes at T2 generation plants. Compared with wild-type (WT) plants, the leaf disks of the SlVKOR overexpression plants retained a much higher percentage of chlorophyll after salt or drought treatment, whereas the antisense transgenic plants displayed an opposite response. The overexpressed plants displayed lower levels of H2O2 and superoxide anion radical (O2(·-)) than WT plants, whereas antisense plants accumulated considerably more H2O2 and O2(·-). The activities of reactive oxygen scavenger enzymes, including superoxide dismutase, peroxidase, ascorbate peroxidase and catalase, were consistent with the accumulation of reactive oxygen species. Based on these results, we suggest that SlVKOR is involved in resistance to salt or drought stress.
authors: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors: Zhi-Bo
authors: ZB
authors: Yu
authors: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors: Xiao-Jian
authors: XJ
authors: Yang
authors: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors: Jia-Jia
authors: JJ
authors: Du
authors: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors: Chun-Mei
authors: CM
authors: Wan
authors: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors: Jia-Ning
authors: JN
authors: Xu
authors: Jinan Academy of Agricultural Sciences, Jinan, Shandong, 250300, People's Republic of China.
authors: Wen-Jun
authors: WJ
authors: Wang
authors: Jinan Academy of Agricultural Sciences, Jinan, Shandong, 250300, People's Republic of China.
authors: Yue-Guang
authors: YG
authors: Feng
authors: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors: Xiao-Yun
authors: XY
authors: Wang
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D055864:Droughts; D004926:Escherichia coli / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D050296:Microbial Viability; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified; D016372:RNA, Antisense / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D013481:Superoxides / Q000378:metabolism; D064417:Vitamin K Epoxide Reductases / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D016372:RNA, Antisense; D013481:Superoxides; D006861:Hydrogen Peroxide; D064417:Vitamin K Epoxide Reductases