title: Taxonomy and molecular phylogeny of two novel ciliates, with establishment of a new genus, Pseudogastrostyla n. g. (Ciliophora, Hypotrichia, Oxytrichidae).
abstract: Two novel hypotrichous ciliates, Pseudogastrostyla flava n. g., n. sp. and Urosomoida subtropica n. sp., isolated from Daya Bay Mangrove Park, Huizhou, southern China, were observed in vivo and after protargol staining. Pseudogastrostyla n. g. is diagnosed by having more than 18 frontal-ventral-transverse cirri, an indistinct frontoventral row, undulating membranes in Oxytricha-pattern and dorsomarginal kinety, but lacking dorsal kinety fragmentation during morphogenesis. The monotypic species, P. flava n. sp., is characterized by its brownish body colour, brown-yellowish cortical granules mostly arranged in groups, frontoventral row terminating near mid-body, and one caudal cirrus. Urosomoida subtropica n. sp. can be recognized by having one micronucleus located between two macronuclear nodules, four transverse cirri, pretransverse ventral cirrus (V/2) anteriorly located, four dorsal kineties and three caudal cirri. Phylogenetic analyses based on SSU rRNA gene sequence data indicate that Pseudogastrostyla n. g. is nested within the oxytrichids and is most closely related to Rubrioxytricha ferruginea, and that Urosomoida subtropica n. sp. clusters with Oxytricha elegans rather than the type species, U. agilis. Nodal support values are, however, too low to draw any conclusions about the molecular systematics of the genus Urosomoida.
authors: Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors: Yangbo
authors: Y
authors: Fan
authors: Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors: Xiaolu
authors: X
authors: Zhao
authors: Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: xiaozhonghu@ouc.edu.cn.
authors: Xiaozhong
authors: X
authors: Hu
authors: College of Life Sciences, Graduate University of Chinese Academy of Sciences, Beijing, China. Electronic address: doublemiao@126.com.
authors: Miao
authors: M
authors: Miao
authors: Department of Life Sciences, Natural History Museum, London SW7 5BD, United Kingdom.
authors: Alan
authors: A
authors: Warren
authors: Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
authors: Weibo
authors: W
authors: Song
mesh_terms: D002681:China; D016810:Hypotrichida / Q000145:classification* / Q000166:cytology / Q000235:genetics; D008857:Microscopy, Interference; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: Phylogeny; Pseudogastrostyla flava n. g., n. sp.; SSU rDNA; Taxonomy; Urosomoida subtropica n. sp.