title: The phylogenetic analysis of fungi associated with lichenized ascomycete genus Bryoria reveals new lineages in the Tremellales including a new species Tremella huuskonenii hyperparasitic on Phacopsis huuskonenii.
abstract: The basidiomycete order Tremellales includes many species parasitic on or cohabiting with lichen-forming fungi. In this study, we examined the phylogenetic position of Tremellales obtained from Bryoria thalli using nSSU, 5.8S, and partial nLSU sequence data. Both Bayesian and maximum likelihood analyses revealed the presence of basidiomycetous fungi in three separate clades within Tremellales. Tremellales sp. A and Tremella sp. B exist asymptomatically in Bryoria thalli and should thus be regarded as endolichenic rather than lichenicolous fungi. The third lineage represents a new species and is described here as Tremella huuskonenii. It is hyperparasitic over galls induced by Phacopsis huuskonenii, a lichenicolous fungus growing in Bryoria thalli. We also examined the genetic diversity of Tremella sp. B and Tremella huuskonenii with an extended taxon sampling using ITS and partial nLSU sequence data. For comparison, ITS, GAPDH, and Mcm7 regions were used for phylogenetic analyses of the host lichen specimens. According to our results, phylogenetic structure within the two Tremella species does not appear to correlate with the geographic distribution nor with the phylogeny or the secondary chemistry of the host lichen. However, ITS haplotype analysis of T. huuskonenii revealed some genetic differences between European and North American populations as some haplotypes were more common in Europe than in North America and vice versa.
authors: Botanical Museum, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014, Finland. Electronic address: hanna.lindgren@helsinki.fi.
authors: Hanna
authors: H
authors: Lindgren
authors: Musée national d'histoire naturelle, 25 rue Munster, L-2160, Luxembourg, Luxembourg.
authors: Paul
authors: P
authors: Diederich
authors: UBC Herbarium, Beaty Museum, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
authors: Trevor
authors: T
authors: Goward
authors: Botanical Museum, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014, Finland.
authors: Leena
authors: L
authors: Myllys
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D008011:Lichens / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Endolichenic fungi; Host-specificity; Lichenicolous fungi; Secondary chemistry