title: Prevalence and genotype distribution of rotaviruses in children with gastroenteritis in Rize province.
abstract: Determination of the distribution of rotavirus genotypes is essential for understanding the epidemiology of this virus responsible for nearly half a million of deaths in patients with gastroenteritis worldwide. In the present study, we aimed to genotype the rotavirus strains isolated from diarrheal stool samples in children under 5 years old. A total of 1297 fecal samples were collected, and rotavirus antigen was detected in 73 of these samples. Antigen-positive samples were transferred to the Public Health Agency of Turkey, Molecular Microbiology Research Laboratory, and were tested for determination of genotypes G and P using semi-nested multiplex polymerase chain reaction method performed with consensus- and genotype-specific primers. Twelve specimens were found to be negative for rotavirus in genotyping method. All the positive-strains were in G1-4, G8-9, P(4), P(8), and P(9) genotypes. The most frequent GP genotype combinations were found to be G9P(8) in 21 strains (34.4%), G2P(4) in 14 strains (23.0%), and G1P(8) in 12 strains (19.7%). We found 10 distinct genotypes amongst a total of 61 strains. Among the strains isolated and genotyped in our study, 90.2% (55/61) and 67.2% (41/61) have already been included in the two existing commercial vaccines. In conclusion, these findings implicate the necessity of development of region-specific vaccines after evaluation of the local genotype distribution. Further studies on the large number of rotavirus strains would contribute to this process.
authors: Department of Pediatrics, School of Medicine Recep Tayyip Erdogan University Rize. dereciselim@hotmail.com.
authors: Selim
authors: S
authors: Dereci
authors: Ayşegül
authors: A
authors: Çopur Çiçek
authors: Sümeyra
authors: S
authors: Savaş Acar
authors: Zekiye
authors: Z
authors: Bakkaloğlu
authors: Serdar
authors: S
authors: Özkasap
authors: Kadri
authors: K
authors: Kanber
authors: Şadan
authors: Ş
authors: Hacisalihoğlu
authors: Yücehan
authors: Y
authors: Albayrak
authors: Rıza
authors: R
authors: Durmaz
mesh_terms: D002675:Child, Preschool; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D015995:Prevalence; D012189:Retrospective Studies; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D014421:Turkey