title: Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.
abstract: Mitochondrial ATP synthase is a motor enzyme in which a central shaft rotates in the stator casings fixed with the peripheral stator stalk. When expression of d-subunit, a stator stalk component, was knocked-down, human cells could not form ATP synthase holocomplex and instead accumulated two subcomplexes, one containing a central rotor shaft plus catalytic subunits (F1-c-ring) and the other containing stator stalk components ("b-e-g" complex). F1-c-ring was also formed when expression of mitochondrial DNA-coded a-subunit and A6L was suppressed. Thus, the central rotor shaft and the stator stalk are formed separately and they assemble later. Similar assembly strategy has been known for ATP synthase of yeast and Escherichia coli and could be common to all organisms.
authors: JST ICORP ATP-Synthesis Regulation Project, 2-3-6 Aomi, Koto-ku, Tokyo 135-0064, Japan; Dep. of Phamacol. and Neurobiol., Grad. School of Med., Tokyo Medical and Dental Univ., 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors: Makoto
authors: M
authors: Fujikawa
authors: JST ICORP ATP-Synthesis Regulation Project, 2-3-6 Aomi, Koto-ku, Tokyo 135-0064, Japan; Dep. of Chemical and Biological Sciences, Faculty of Science, Japan Women's Univ., 2-8-1 Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan; Dep. of Molecular Bioscience, Faculty of Life Sciences, Kyoto Sangyo Univ., Kamigamo-Motoyama, Kyoto 603-8555, Japan.
authors: Kanako
authors: K
authors: Sugawara
authors: Dep. of Phamacol. and Neurobiol., Grad. School of Med., Tokyo Medical and Dental Univ., 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors: Tsutomu
authors: T
authors: Tanabe
authors: JST ICORP ATP-Synthesis Regulation Project, 2-3-6 Aomi, Koto-ku, Tokyo 135-0064, Japan; Dep. of Molecular Bioscience, Faculty of Life Sciences, Kyoto Sangyo Univ., Kamigamo-Motoyama, Kyoto 603-8555, Japan. Electronic address: masasuke.yoshida@cc.kyoto-su.ac.jp.
authors: Masasuke
authors: M
authors: Yoshida
mesh_terms: D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D066329:Protein Aggregates; D021122:Protein Subunits / Q000172:deficiency / Q000235:genetics
chemical_list: D024101:Mitochondrial Proteins; D066329:Protein Aggregates; D021122:Protein Subunits; D025261:Mitochondrial Proton-Translocating ATPases
keywords: ATP synthase; ATP5H; Assembly; F(o)F(1); Mitochondrial activity of SLO-permeabilized cells assay; Stator stalk; d-Subunit