title: Application of Deamidated Gliadin Antibodies in the Follow-Up of Treated Celiac Disease.
abstract: BACKGROUND
  The role of serological tests such as IgA anti-transglutaminase autoantibodies has become increasingly important in celiac disease (CD) diagnosis. However, the efficiency of these tests for patient follow-up is controversial. We investigated the correlation of 12 different serological tests, including recent deamidated gliadin and actin IgA tests, with villous atrophy (VA) in a retrospective cohort of treated celiac patients.
  
  
  METHODS
  Serum samples were collected from 100 treated CD patients who had intestinal biopsy in the course of their follow-up. Antibodies against transglutaminase, deamidated gliadin peptides, and native gliadin were measured, along with IgA anti-actin. The biopsy slides were all blind-reviewed and scored according to Marsh classification.
  
  
  RESULTS
  For all deamidated gliadin and transglutaminase tests, we found that a positive result was significantly associated with persistence of intestinal VA, with a diagnostic efficacy up to 80%. Furthermore, antibodies titers directly correlated with the degree of VA, indicating a strong link between disease activity and presence of antibodies in the serum. Interestingly, the tests with the highest association with persistent VA were those for deamidated gliadin IgG. Using a test positivity pattern analysis, we were also able to identify several groups of patients with distinct antibody profiles that showed significant differences in intestinal damage and diet compliance.
  
  
  CONCLUSIONS
  Altogether, these results show that deamidated gliadin antibodies are strongly correlated with VA and should be considered valuable tools in CD follow-up and that multiplex serologic analysis for treated CD represents a promising tool for personalized patient management.
authors: Immunology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France; INSERM UMR 996, Paris Sud University, Châtenay-Malabry, France.
authors: Luc
authors: L
authors: de Chaisemartin
authors: Pathology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors: Tchao
authors: T
authors: Meatchi
authors: Gastroenterology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France; Paris Descartes University, Paris, France.
authors: Georgia
authors: G
authors: Malamut
authors: Immunology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors: Fahima
authors: F
authors: Fernani-Oukil
authors: Immunology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors: Frédérique
authors: F
authors: Hosking
authors: Gastroenterology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors: Dorothée
authors: D
authors: Rault
authors: Immunology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors: Fabienne
authors: F
authors: Bellery
authors: Gastroenterology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France; Paris Descartes University, Paris, France.
authors: Christophe
authors: C
authors: Cellier
authors: Immunology Department, Georges Pompidou European Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France; Paris Descartes University, Paris, France.
authors: Marie-Agnès
authors: MA
authors: Dragon-Durey
mesh_terms: D000199:Actins / Q000276:immunology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D002446:Celiac Disease / Q000276:immunology* / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D005903:Gliadin / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D011503:Transglutaminases / Q000276:immunology; D055815:Young Adult
chemical_list: D000199:Actins; D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin; D011503:Transglutaminases