title: Aestuariivita atlantica sp. nov., isolated from deep-sea sediment.
abstract: A novel strain, 22II-S11-z3T, was isolated from the deep-sea sediment of the Atlantic Ocean. The bacterium was aerobic, Gram-staining-negative, oxidase-positive and catalase-negative, oval- to rod-shaped, and non-motile. Growth was observed at salinities of 1-9 % NaCl and temperatures of 10-45 °C. The isolate could hydrolyse aesculin and Tweens 20, 40 and 80, but not gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z3T belonged to the genus Aestuariivita, with highest sequence similarity to Aestuariivita boseongensis KCTC 42052T (97.5 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain 22II-S11-z3T and A. boseongensis KCTC 42052T were 71.5 % and 20.0 ± 2.3 %, respectively. The G+C content of the chromosomal DNA was 65.5 mol%. The principal fatty acids (>5 %) were summed feature 8 (C18 : 1ω7c/ω6c) (35.2 %), C19 : 0 cyclo ω8c (20.9 %), C16 : 0 (11.8 %), 11-methyl C18 : 1ω7c (11.4 %) and C12 : 1 3-OH (9.4 %). The respiratory quinone was determined to be Q-10. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, nine unidentified phospholipids, one unidentified aminolipid and two unidentified lipids were present. The combined genotypic and phenotypic data show that strain 22II-S11-z3T represents a novel species of the genus Aestuariivita, for which the name Aestuariivita atlantica sp. nov. is proposed, with the type strain 22II-S11-z3T ( = KCTC 42276T = MCCC 1A09432T).
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
authors: Guizhen
authors: G
authors: Li
authors: Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
authors: Qiliang
authors: Q
authors: Lai
authors: South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
authors: Yaping
authors: Y
authors: Du
authors: South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
authors: Xiupian
authors: X
authors: Liu
authors: South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
authors: Fengqin
authors: F
authors: Sun
authors: Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, PR China.
authors: Zongze
authors: Z
authors: Shao
mesh_terms: D001267:Atlantic Ocean; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2