title: The effect of zinc and phytoestrogen supplementation on the changes in mineral content of the femur of rats with chemically induced mammary carcinogenesis.
abstract: The aim of this study was to assess skeletal effects of zinc or zinc with phytoestrogen (resveratrol or genistein) supplementation in an animal model of rats with DMBA-induced mammary carcinogenesis. The changes in bone parameters such as the length and mass were examined, as well as the changes in concentrations of selected minerals: calcium, magnesium, zinc, iron and phosphorus. Moreover, the investigations focused on finding the differences between the levels of iron and zinc in other tissues: the liver, spleen and serum of the examined rats. Fifty-six female Sprague-Dawley rats, 40 days old, were divided into four groups, regardless of the diets: standard (77mg Zn kg/food), zinc (4.6mg/mL via gavage), zinc (4.6mg/mL) plus resveratrol (0.2mg/kgbw), and zinc (4.6mg/mL) plus genistein (0.2mg/kgbw) for a period from 40 days until 20 weeks of age. The study rats were also treated with 7,12-dimethyl-1,2-benz[a]anthracene (DMBA) to induce mammary carcinogenesis. The applied diet and the advanced mammary cancer did not affect macrometric parameters of the rats' bones, but they strongly affected their mineral content. It was found that mammary cancer, irrespectively of the applied diet, significantly modified the iron level in the femur, liver, spleen and serum of the examined rats. In addition, zinc supplementation significantly lowered the levels of calcium, magnesium and phosphorus in the femur of rats with mammary cancer as compared with respective levels in the control group. So, it was found that additional supplementation with zinc, which is generally considered to be an antioxidant, with the co-existing mammary carcinoma, increased the unfavorable changes as concerns the stability of bone tissue. The appropriate combination of zinc and phytoestrogens (resveratrol or genistein) could help prevent or slow bone loss associated with a range of skeletal disorders in breast cancer.
authors: Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors: Dorota
authors: D
authors: Skrajnowska
authors: Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland. Electronic address: barbara.bobrowska@wum.edu.pl.
authors: Barbara Bobrowska-
authors: BB
authors: Korczak
authors: Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors: Andrzej
authors: A
authors: Tokarz
authors: Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors: Agata
authors: A
authors: Kazimierczuk
authors: Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors: Marta
authors: M
authors: Klepacz
authors: Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors: Justyna
authors: J
authors: Makowska
authors: Division of Rehabilitation, Department of Physiotherapy, 2nd Medical Faculty, Medical University of Warsaw, Poland.
authors: Blazej
authors: B
authors: Gadzinski
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000704:Analysis of Variance; D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D005163:Factor Analysis, Statistical; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007501:Iron / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D048789:Phytoestrogens / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D015032:Zinc / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D008903:Minerals; D048789:Phytoestrogens; D015127:9,10-Dimethyl-1,2-benzanthracene; D007501:Iron; D015032:Zinc
keywords: Bioelements; Breast cancer; Femur; Rat model; Zinc and phytoestrogens supplementation