title: Influence of different oocyte insemination techniques on early and late morphokinetic parameters: retrospective analysis of 500 time-lapse monitored blastocysts.
abstract: OBJECTIVE
  To determine how standard IVF vs. intracytoplasmic sperm injection (ICSI) fertilization influences early and late morphokinetic parameters during prolonged embryo culture.
  
  
  METHODS
  Five-hundred expanded blastocysts that were monitored in a time-lapse monitoring incubator were analysed retrospectively. Early (pronuclear fading [PNf], t2-t9) and late (start of blastulation, expanded blastocyst) morphokinetic variables were scored according to published consensus criteria.
  
  
  METHODS
  Private infertility clinic.
  
  
  METHODS
  A total of 209 consecutive infertile patients (mean ± SD age, 38.4 ± 4 years; range, 28-47 years) undergoing 238 natural IVF/minimal ovarian stimulation cycles during 2012-2014.
  
  
  METHODS
  Minimal ovarian stimulation, oocyte retrieval, fertilization with standard IVF or ICSI, prolonged embryo culture in a time-lapse monitoring incubator.
  
  
  METHODS
  Differences in morphokinetic parameters according to insemination techniques.
  
  
  RESULTS
  In total, 29% and 71% of the whole cohort was fertilized with standard IVF and ICSI, respectively. During early cleavage stages (PNf to t4) there was a statistically significant delay (+1.5 to +1.1 hours) among IVF-fertilized embryos. By contrast, at the expanded blastocyst stage IVF-fertilized embryos showed faster development (-3.3 to -4.1 hours). After normalizing to the time point of PNf, differences in cleavage-stage parameters disappeared, but those at all blastocyst stages increased even further in favor of IVF-fertilized embryos (-3.2 to -5.7 hours).
  
  
  CONCLUSIONS
  The observed 1.5-hour time difference between standard IVF- and ICSI-fertilized embryos is an artificial phenomenon. At the blastocyst stages, however, genuine timing differences arise between IVF- and ICSI-fertilized embryos, possibly related to their different quality. Normalization to a common time point permits the joint analysis of IVF- and ICSI-fertilized embryos, thus increasing the size of studied cohorts.
authors: Kobe Motomachi Yume Clinic, Kobe, Japan. Electronic address: bodridaniel@gmail.com.
authors: Daniel
authors: D
authors: Bodri
authors: Kobe Motomachi Yume Clinic, Kobe, Japan.
authors: Takeshi
authors: T
authors: Sugimoto
authors: Kobe Motomachi Yume Clinic, Kobe, Japan.
authors: Jazmina Yao
authors: JY
authors: Serna
authors: Kobe Motomachi Yume Clinic, Kobe, Japan.
authors: Masae
authors: M
authors: Kondo
authors: Kobe Motomachi Yume Clinic, Kobe, Japan.
authors: Ryutaro
authors: R
authors: Kato
authors: Kobe Motomachi Yume Clinic, Kobe, Japan.
authors: Satoshi
authors: S
authors: Kawachiya
authors: Kobe Motomachi Yume Clinic, Kobe, Japan.
authors: Tsunekazu
authors: T
authors: Matsumoto
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000502:physiology*; D046149:Embryo Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007700:Kinetics; D008297:Male; D018715:Microscopy, Video; D008875:Middle Aged; D009024:Morphogenesis; D054315:Oocyte Retrieval; D010062:Ovulation Induction; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D059008:Time-Lapse Imaging
keywords: ICSI; Time-lapse monitoring; blastocyst culture; in vitro fertilization; single-embryo transfer