title: MITF is a critical regulator of the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) in malignant melanoma.
abstract: The multifunctional Ig-like carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is neo-expressed in the majority of malignant melanoma lesions. CEACAM1 acts as a driver of tumor cell invasion, and its expression correlates with poor patient prognosis. Despite its importance in melanoma progression, how CEACAM1 expression is regulated is largely unknown. Here, we show that CEACAM1 expression in melanoma cell lines and melanoma tissue strongly correlates with that of the microphthalmia-associated transcription factor (MITF), a key regulator of melanoma proliferation and invasiveness. MITF is revealed as a direct and positive regulator for CEACAM1 expression via binding to an M-box motif located in the CEACAM1 promoter. Taken together, our study provides novel insights into the regulation of CEACAM1 expression and suggests an MITF-CEACAM1 axis as a potential determinant of melanoma progression.
authors: Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, Essen, Germany.|German Cancer Consortium (DKTK), Essen, Germany.
authors: Nico
authors: N
authors: Ullrich
authors: Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, Essen, Germany.|German Cancer Consortium (DKTK), Essen, Germany.
authors: Stefanie
authors: S
authors: Löffek
authors: Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, Essen, Germany.|German Cancer Consortium (DKTK), Essen, Germany.
authors: Susanne
authors: S
authors: Horn
authors: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch-Graffenstaden, France.
authors: Marie
authors: M
authors: Ennen
authors: Nuffield Department of Clinical Medicine, Ludwig Institute for Cancer Research, University of Oxford, Headington, Oxford, UK.
authors: Luis
authors: L
authors: Sánchez-Del-Campo
authors: Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, Essen, Germany.|German Cancer Consortium (DKTK), Essen, Germany.
authors: Fang
authors: F
authors: Zhao
authors: German Cancer Consortium (DKTK), Essen, Germany.|Department of Medical Oncology, West German Cancer Center, Essen, Germany.
authors: Frank
authors: F
authors: Breitenbuecher
authors: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch-Graffenstaden, France.
authors: Irwin
authors: I
authors: Davidson
authors: Institute of Anatomy, University Hospital, University Duisburg-Essen, Essen, Germany.
authors: Bernhard B
authors: BB
authors: Singer
authors: Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, Essen, Germany.|German Cancer Consortium (DKTK), Essen, Germany.
authors: Dirk
authors: D
authors: Schadendorf
authors: Nuffield Department of Clinical Medicine, Ludwig Institute for Cancer Research, University of Oxford, Headington, Oxford, UK.
authors: Colin R
authors: CR
authors: Goding
authors: Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, Essen, Germany.|German Cancer Consortium (DKTK), Essen, Germany.
authors: Iris
authors: I
authors: Helfrich
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism*; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism*; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs / Q000235:genetics; D011485:Protein Binding; D012878:Skin Neoplasms; D000096142:Cutaneous Malignant Melanoma
chemical_list: D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D051739:Microphthalmia-Associated Transcription Factor
keywords: M-box motif; carcinoembryonic antigen-related cell adhesion molecule 1; melanoma; microphthalmia-associated transcription factor