title: Protein kinase D promotes plasticity-induced F-actin stabilization in dendritic spines and regulates memory formation.
abstract: Actin turnover in dendritic spines influences spine development, morphology, and plasticity, with functional consequences on learning and memory formation. In nonneuronal cells, protein kinase D (PKD) has an important role in stabilizing F-actin via multiple molecular pathways. Using in vitro models of neuronal plasticity, such as glycine-induced chemical long-term potentiation (LTP), known to evoke synaptic plasticity, or long-term depolarization block by KCl, leading to homeostatic morphological changes, we show that actin stabilization needed for the enlargement of dendritic spines is dependent on PKD activity. Consequently, impaired PKD functions attenuate activity-dependent changes in hippocampal dendritic spines, including LTP formation, cause morphological alterations in vivo, and have deleterious consequences on spatial memory formation. We thus provide compelling evidence that PKD controls synaptic plasticity and learning by regulating actin stability in dendritic spines.
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
authors: Norbert
authors: N
authors: Bencsik
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
authors: Zsófia
authors: Z
authors: Szíber
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
authors: Hanna
authors: H
authors: Liliom
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
authors: Krisztián
authors: K
authors: Tárnok
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
authors: Sándor
authors: S
authors: Borbély
authors: MTA-ELTE-NAP B Neuronal Cell Biology Research Group, H-1117 Budapest, Hungary.
authors: Márton
authors: M
authors: Gulyás
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
authors: Anikó
authors: A
authors: Rátkai
authors: MTA-ELTE-NAP B Neuronal Cell Biology Research Group, H-1117 Budapest, Hungary.
authors: Attila
authors: A
authors: Szűcs
authors: Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, Hungary.
authors: Diána
authors: D
authors: Hazai-Novák
authors: Institute of Cell Biology and Immunology, University of Stuttgart, D-70569 Stuttgart, Germany.
authors: Kornelia
authors: K
authors: Ellwanger
authors: Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, Hungary.
authors: Bence
authors: B
authors: Rácz
authors: Institute of Cell Biology and Immunology, University of Stuttgart, D-70569 Stuttgart, Germany.
authors: Klaus
authors: K
authors: Pfizenmaier
authors: Institute of Cell Biology and Immunology, University of Stuttgart, D-70569 Stuttgart, Germany.
authors: Angelika
authors: A
authors: Hausser
authors: Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary MTA-ELTE-NAP B Neuronal Cell Biology Research Group, H-1117 Budapest, Hungary schlettk@ludens.elte.hu.
authors: Katalin
authors: K
authors: Schlett
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000378:metabolism; D056651:CA2 Region, Hippocampal / Q000166:cytology / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000378:metabolism*; D005998:Glycine / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007858:Learning / Q000502:physiology; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology; D008568:Memory / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology; D009473:Neuronal Plasticity / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011189:Potassium Chloride / Q000494:pharmacology; D011493:Protein Kinase C / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D000199:Actins; D009467:Neuromuscular Depolarizing Agents; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011189:Potassium Chloride; C089212:protein kinase D; D011493:Protein Kinase C; D005998:Glycine