title: PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persica L. Batsch), participates in IAA biosynthesis during fruit ripening.
abstract: High concentrations of indole-3-acetic acid (IAA) are required for climacteric ethylene biosynthesis to cause fruit softening in melting flesh peaches at the late ripening stage. By contrast, the fruits of stony hard peach cultivars do not soften and produce little ethylene due to the low IAA concentrations. To investigate the regulation of IAA accumulation during peach ripening [the transition from stage S3 to stage S4 III (climacteric)], a digital gene expression (DGE) analysis was performed. The expression patterns of auxin-homeostasis-related genes were compared in fruits of the melting flesh peach 'Goldhoney 3' and the stony hard flesh peach 'Yumyeong' during the ripening stage. It is revealed here that a YUCCA flavin mono-oxygenase gene (PpYUC11, ppa008176m), a key gene in auxin biosynthesis, displayed an identical differential expression profile to the profiles of IAA accumulation and PpACS1 transcription: the mRNA transcripts increased at the late ripening stage in melting flesh peaches but were below the limit of detection in mature fruits of stony hard peaches. In addition, the strong association between intron TC microsatellite genotypes of PpYUC11 and the flesh texture (normal or stony hard) is described in 43 peach varieties, indicating that this locus may be responsible for the stony hard phenotype in peach. These findings support the hypothesis that PpYUC11 may play an essential role in auxin biosynthesis during peach fruit ripening and is a candidate gene for the control of the stony hard phenotype in peach.
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Lei
authors: L
authors: Pan
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Wenfang
authors: W
authors: Zeng
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Liang
authors: L
authors: Niu
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Zhenhua
authors: Z
authors: Lu
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Hui
authors: H
authors: Liu
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Guochao
authors: G
authors: Cui
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Yunqin
authors: Y
authors: Zhu
authors: National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Jinfang
authors: J
authors: Chu
authors: National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Weiping
authors: W
authors: Li
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Weichao
authors: W
authors: Fang
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
authors: Zuguo
authors: Z
authors: Cai
authors: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China wang6399@126.com liguohuai@mail.hzau.edu.cn.
authors: Guohuai
authors: G
authors: Li
authors: Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China wang6399@126.com liguohuai@mail.hzau.edu.cn.
authors: Zhiqiang
authors: Z
authors: Wang
mesh_terms: D005638:Fruit / Q000254:growth & development*; D017343:Genes, Plant; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D000068239:Prunus persica / Q000235:genetics* / Q000254:growth & development*
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins; C030737:indoleacetic acid; D010105:Oxygenases
keywords: Auxin; Prunus persica L. Batsch; YUCCA flavin monooxygenase.; ethylene; microsatellite; ripening; stony hard