title: Modulation of protein behavior through light responses of TiO2 nanodots films.
abstract: In this work, the behavior of protein molecules adsorbed on TiO2 nanodots films are modulated through the light responses of the nanodots. TiO2 nanodots films are first prepared through phase separation induced self assembly. Then, bovine serum albumin (BSA) is adsorbed on TiO2 nanodots films and exposed to ultraviolet (365 nm) illumination. It is found the conformation of surface-bound BSA molecules changes with ultraviolet illumination. Moreover, the BSA molecules conjugate to the surface-bound molecules, which are in the overlayer, are released. The reason is ascribed to that TiO2 nanodots absorb ultraviolet and result in the increase of surface hydroxyl groups on nanodots. Such increase further leads to intensified attraction of -NH3 groups in the surface-bound BSA molecules. That not only changes the conformation of the surface-bound BSA molecules, but also weaken the conjugation between surface-bound molecules and other BSA molecules in the overlayer. Eventually, the overlayer of BSA molecules is released. It is believed that such protein conformation variation and release behavior induced through light responses of TiO2 nanodots are crucial in understanding the biomedical performance of TiO2 nanostructures. Also, it could be widely utilized in tailoring of the materials-protein interactions.
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors: Kui
authors: K
authors: Cheng
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors: Yi
authors: Y
authors: Hong
authors: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China.|The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310003, China.
authors: Mengfei
authors: M
authors: Yu
authors: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310003, China.
authors: Jun
authors: J
authors: Lin
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.|The Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
authors: Wenjian
authors: W
authors: Weng
authors: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China.|The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310003, China.
authors: Huiming
authors: H
authors: Wang
mesh_terms: D000327:Adsorption / Q000528:radiation effects; D001665:Binding Sites / Q000528:radiation effects; D008027:Light; D008422:Materials Testing; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects*; D010316:Particle Size; D011485:Protein Binding / Q000528:radiation effects; D011487:Protein Conformation / Q000528:radiation effects; D011829:Radiation Dosage; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D008567:Membranes, Artificial; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium