title: Bio-Control of Salmonella Enteritidis in Foods Using Bacteriophages.
abstract: Two lytic phages, vB_SenM-PA13076 (PA13076) and vB_SenM-PC2184 (PC2184), were isolated from chicken sewage and characterized with host strains Salmonella Enteritidis (SE) ATCC13076 and CVCC2184, respectively. Transmission electron microscopy revealed that they belonged to the family Myoviridae. The lytic abilities of these two phages in liquid culture showed 104 multiplicity of infection (MOI) was the best in inhibiting bacteria, with PC2184 exhibiting more activity than PA13076. The two phages exhibited broad host range within the genus Salmonella. Phage PA13076 and PC2184 had a lytic effect on 222 (71.4%) and 298 (95.8%) of the 311 epidemic Salmonella isolates, respectively. We tested the effectiveness of phage PA13076 and PC2184 as well as a cocktail combination of both in three different foods (chicken breast, pasteurized whole milk and Chinese cabbage) contaminated with SE. Samples were spiked with 1 × 10(4) CFU individual SE or a mixture of strains (ATCC13076 and CVCC2184), then treated with 1 × 10(8) PFU individual phage or a two phage cocktail, and incubated at 4 °C or 25 °C for 5 h. In general, the inhibitory effect of phage and phage cocktail was better at 4 °C than that at 25 °C, whereas the opposite result was observed in Chinese cabbage, and phage cocktail was better than either single phage. A significant reduction in bacterial numbers (1.5-4 log CFU/sample, p < 0.05) was observed in all tested foods. The two phages on the three food samples were relatively stable, especially at 4 ºC, with the phages exhibiting the greatest stability in milk. Our research shows that our phages have potential effectiveness as a bio-control agent of Salmonella in foods.
authors: Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, State Key Laboratory Cultivation Base of MOST- Jiangsu Key Laboratory of Food Quality and Safety, Nanjing 210014, China. baohongduo@163.com.
authors: Hongduo
authors: H
authors: Bao
authors: Ginling College, Nanjing Normal University, Nanjing 210097, China. panpan.jump@163.com.
authors: Pengyu
authors: P
authors: Zhang
authors: Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, State Key Laboratory Cultivation Base of MOST- Jiangsu Key Laboratory of Food Quality and Safety, Nanjing 210014, China. zh851200@163.com.
authors: Hui
authors: H
authors: Zhang
authors: Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, State Key Laboratory Cultivation Base of MOST- Jiangsu Key Laboratory of Food Quality and Safety, Nanjing 210014, China. zhou.yan.77@hotmail.com.
authors: Yan
authors: Y
authors: Zhou
authors: Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, State Key Laboratory Cultivation Base of MOST- Jiangsu Key Laboratory of Food Quality and Safety, Nanjing 210014, China. lilizhangnj@163.com.
authors: Lili
authors: L
authors: Zhang
authors: Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, State Key Laboratory Cultivation Base of MOST- Jiangsu Key Laboratory of Food Quality and Safety, Nanjing 210014, China. ranwang@jaas.ac.cn.
authors: Ran
authors: R
authors: Wang
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001433:Bacteriolysis; D029741:Brassica rapa / Q000382:microbiology; D002645:Chickens; D005516:Food Microbiology; D059022:Food Safety / Q000379:methods*; D058507:Host Specificity; D008460:Meat / Q000382:microbiology; D046529:Microscopy, Electron, Transmission; D008892:Milk / Q000382:microbiology; D017900:Myoviridae / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000648:ultrastructure; D012483:Salmonella Phages / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000648:ultrastructure; D012477:Salmonella enteritidis / Q000502:physiology* / Q000821:virology*; D012722:Sewage / Q000821:virology; D013696:Temperature; D013997:Time Factors; D014771:Virion / Q000648:ultrastructure
chemical_list: D012722:Sewage
keywords: Salmonella Enteritidis (SE); bio-control; food; phage cocktail; phages