title: Treatment of Acute Vocal Fold Injury With Platelet-Rich Plasma.
abstract: OBJECTIVE
  Platelet-rich plasma (PRP) is a reliable and has low side-effect profile and has beneficial effects on wound healing. Its investigatory effects on wound-healing process were shown on various tissues. The aim of the present study was to evaluate effectiveness of PRP application on scar tissue of acute vocal fold injury.
  
  
  METHODS
  Twenty-four Wistar rats were used in the study. The entire layer of the lamina propria down to the thyroarytenoid muscle of 10 subjects was unilaterally injured by with a microscissor. Gelfoam-absorbed PRP was applied on the injured area for 10 minutes. Control group consisted of rats unilaterally injured using a microscissor, and gelfoam with normal saline was applied on the injured area. Following sacrifice, the larynxes were carefully dissected and removed for histopathologic examination. After excised larynx experiments, serial sections were prepared from vocal fold. Hematoxylin eosin and immunohistochemical staining were done for epithelial growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR1), and vascular endothelial growth factor (VEGF) staining for histopathologic examinations.
  
  
  RESULTS
  There was not a significant difference between the two groups for lymphocyte. Although collagen and VEGF were higher in the study group, there was not a significant difference between the groups (P > 0.05). There was a significant difference between control and study groups for EGFR and FGFR1(P < 0.05).
  
  
  CONCLUSIONS
  PRP has beneficial effects on wound healing. PRP accelerates epithelization of injured rat vocal folds by inducing EGFR secretion. PRP is an autogenous, reliable, low side-effect profile, easily harvested material. PRP may be useful to prevent scar formation.
authors: Department of Otolaryngology, Selcuklu Faculty of Medicine, Selcuk University, Konya, Turkey. Electronic address: serapbulut88@mynet.com.
authors: Serap Bulut
authors: SB
authors: Cobden
authors: Department of Otolaryngology, Selcuklu Faculty of Medicine, Selcuk University, Konya, Turkey.
authors: Kayhan
authors: K
authors: Oztürk
authors: Department of Human Histology and Embryology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors: Selcuk
authors: S
authors: Duman
authors: Department of Pathology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors: Hasan
authors: H
authors: Esen
authors: Department of Human Histology and Embryology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors: Tahsin Murad
authors: TM
authors: Aktan
authors: Department of Pathology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors: Mustafa Cihat
authors: MC
authors: Avunduk
authors: Department of Otolaryngology, Selcuklu Faculty of Medicine, Selcuk University, Konya, Turkey.
authors: Cagdas
authors: C
authors: Elsurer
mesh_terms: D000208:Acute Disease; D000818:Animals; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D066246:ErbB Receptors / Q000378:metabolism; D007818:Laryngeal Diseases / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D053657:Platelet-Rich Plasma; D017208:Rats, Wistar; D062326:Re-Epithelialization; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism; D012038:Regeneration; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014827:Vocal Cords / Q000293:injuries* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D003094:Collagen; C483803:Egfr protein, rat; D066246:ErbB Receptors; C496350:Fgfr1 protein, rat; D051496:Receptor, Fibroblast Growth Factor, Type 1
keywords: EGFR; FGFR1; Injury; PRP; Scar; VEGF; Vocal fold