title: Thioaptamer-conjugated CD44-targeted delivery system for the treatment of breast cancer in vitro and in vivo.
abstract: The high transfection efficiency and enhanced therapeutic effect of drug delivery systems developed in recent years imply that ligand-decorated nanocarriers are potentially targeted vectors for breast cancer treatment. Thioaptamer (TA)-modified nanoparticles (NPs) designed in this study mainly consisted of ligand TA and dendritic polyamidoamine (PAMAM). Knowing that TA can bind to CD44-receptors in breast cancer, this study was intended to validate the safety and feasibility of systemic miRNA delivery to breast cancer cells by TA-PEG-PAMAM/miRNA (polyethylene glycol - PEG), testify its tumor targeting efficiency in vitro, and observe its biodistribution when it was administered systemically to a xenograft mouse model of breast cancer. The in vivo and ex vivo imaging results in human breast cancer tumor-bearing mice showed that TA-modification was able to enhance the accumulation of NPs in the breast cancer tumor. Our data showed that TA-NPs did not induce functional impairment to normal tissues and vital organs. TA-NPs may prove to be a safe and effective miRNA deliver system for breast cancer treatment, and could be widely used in pre-clinical and eventually clinical arenas of breast cancer treatment.
authors: a Department of General Surgery , Wuhan General Hospital of Guangzhou Military Command Region , Wuhan , People's Republic of China .|b Department of Pharmaceutics , CPLA No. 425 Hospital , Sanya , People's Republic of China .
authors: Wei
authors: W
authors: Fan
authors: c Department of Pharmaceutics , CPLA No. 98 Hospital , Huzhou , People's Republic of China , and.
authors: Xiang
authors: X
authors: Wang
authors: d Department of Pharmaceutics , Jiaxing University School of Medicine , Jiaxing , People's Republic of China.
authors: Baoyue
authors: B
authors: Ding
authors: b Department of Pharmaceutics , CPLA No. 425 Hospital , Sanya , People's Republic of China .
authors: Haimin
authors: H
authors: Cai
authors: b Department of Pharmaceutics , CPLA No. 425 Hospital , Sanya , People's Republic of China .
authors: Xudong
authors: X
authors: Wang
authors: b Department of Pharmaceutics , CPLA No. 425 Hospital , Sanya , People's Republic of China .
authors: Yueqi
authors: Y
authors: Fan
authors: a Department of General Surgery , Wuhan General Hospital of Guangzhou Military Command Region , Wuhan , People's Republic of China .
authors: Yong
authors: Y
authors: Li
authors: a Department of General Surgery , Wuhan General Hospital of Guangzhou Military Command Region , Wuhan , People's Republic of China .
authors: Shenghui
authors: S
authors: Liu
authors: b Department of Pharmaceutics , CPLA No. 425 Hospital , Sanya , People's Republic of China .
authors: Suifeng
authors: S
authors: Nie
authors: a Department of General Surgery , Wuhan General Hospital of Guangzhou Military Command Region , Wuhan , People's Republic of China .
authors: Qiping
authors: Q
authors: Lu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D050091:Dendrimers / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008856:Microscopy, Fluorescence; D058990:Molecular Targeted Therapy; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; C497483:CD44 protein, human; D050091:Dendrimers; D004337:Drug Carriers; D018960:Hyaluronan Receptors; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C104700:PAMAM Starburst; D011092:Polyethylene Glycols
keywords: Breast cancer; CD44; microRNA; targeted delivery; thioaptamer