title: Effects of Nano-particles on Histo-pathological changes of the fish.
abstract: Regarding fast development of the nanotechnology and the probably of it's side effects on aquatic body organs, this study investigate the effects of nanosilver administration on histology of gill, kidney and biochemical parameters in common carp. The silver nanoparticles were synthesized in a one-step reduction process in an aqueous solution. 60 O. mykiss were obtained from a local commercial hatchery. Fish were divided randomly into four groups. Control group was kept in dechlorinated tap water without any add-on material. Experimental groups were exposed to concentration of 3, 300 and 1000 mg/L of nanosilver solution for eight weeks, respectively. Biochemical analyses of sera, histological alterations of the gill and kidney tissue were done. Aneurism in the secondary lamellae and hyperplasia of epithelium in gills, adhesion of the gill lamellae, inducing hyaline cast formation, significant decreasing in the glomerular diameter and formation of intra cytoplasmic vacuoles in the various urinary tubules were seen in experimental groups. The serum levels of total protein was decreased significantly (P < 0.05) by increasing nanosilver concentration but ALP, LDH, AST and ALT increased significantly (P < 0.05). It is concluded that nanosilver induces gill and kidney damages and changes the biochemical parameters of O. mykiss juveniles in different concentrations.
authors: Faculty of Para-Veterinary Medicine, University of Ilam, Ilam, Iran.
authors: Ali Louei
authors: AL
authors: Monfared
authors: Faculty of Para-Veterinary Medicine, University of Ilam, Ilam, Iran.
authors: Ali Mohammad
authors: AM
authors: Bahrami
authors: Faculty of Para-Veterinary Medicine, University of Ilam, Ilam, Iran.
authors: Ehsan
authors: E
authors: Hosseini
authors: Master of Sciences of Histology, Faculty of Veterinary Medicine, University of Shahid Chamran Ahwaz, Ahwaz, Iran.
authors: Salman
authors: S
authors: Soltani
authors: Department of Parasitology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran.
authors: Minoo
authors: M
authors: Shaddel
keywords: Biochemistry; Carp; Gill; Histology; Kidney; Nanosilver