title: Effect of acetochlor on transcription of genes associated with oxidative stress, apoptosis, immunotoxicity and endocrine disruption in the early life stage of zebrafish.
abstract: The study presented here aimed to characterize the effects of acetochlor on expression of genes related to endocrine disruption, oxidative stress, apoptosis and immune system in zebrafish during its embryo development. Different trends in gene expression were observed after exposure to 50, 100, 200μg/L acetochlor for 96h. Results demonstrated that the transcription patterns of many key genes involved in the hypothalamic-pituitary-gonadal/thyroid (HPG/HPT) axis (e.g., VTG1, ERβ1, CYP19a and TRα), cell apoptosis pathway (e.g., Bcl2, Bax, P53 and Cas8), as well as innate immunity (e.g., CXCL-C1C, IL-1β and TNFα) were affected in newly hatched zebrafish after exposure to acetochlor. In addition, the up-regulation of CAT, GPX, GPX1a, Cu/Zn-SOD and Ogg1 suggested acetochlor might trigger oxidative stress in zebrafish. These finding indicated that acetochlor could simultaneously induce multiple responses during zebrafish embryonic development, and bidirectional interactions among oxidative stress, apoptosis pathway, immune and endocrine systems might be present.
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China. Electronic address: jinhuajiang85@163.com.
authors: Jinhua
authors: J
authors: Jiang
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China.
authors: Shenggan
authors: S
authors: Wu
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China.
authors: Xinju
authors: X
authors: Liu
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China.
authors: Yanhua
authors: Y
authors: Wang
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China.
authors: Xuehua
authors: X
authors: An
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China.
authors: Leiming
authors: L
authors: Cai
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198 Shi Qiao Rd, Hangzhou 310021, Zhejiang, PR China. Electronic address: zhaoxueping@tom.com.
authors: Xueping
authors: X
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D004625:Embryo, Nonmammalian / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006066:Gonads / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D007113:Immunity, Innate / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D014052:Toluidines / Q000633:toxicity*; D014158:Transcription, Genetic / Q000187:drug effects*; D015854:Up-Regulation; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
chemical_list: D052244:Endocrine Disruptors; D006540:Herbicides; D014052:Toluidines; C043377:acetochlor
keywords: Acetochlor; Cell apoptosis; Endocrine disruption; Immunotoxicity; Oxidative stress; Zebrafish