title: [A novel trichostatin analogue culture of Streptomyces sp. CPCC 203909].
abstract: By using a cell-based high throughput screening model for the CLA-1 up-regulator, Streptomyces 203909 was found to produce up-regulator of CLA-1. A novel trichostatin analogue was isolated from the rice fermentation of Streptomyces sp. CPCC 203909by a combination of various chromatographic techniques including column chromatography (CC) over silica gel, flash C18 CC, and reversed-phase HPLC. Its structure was identified as (-)-(R,2E,4Z)-7-[(4'-dimethylamino) phenyl]-4,6-dimethyl-7-oxohepta-2,4-dienoyl-L-glutamine (1) by the spectroscopic and chemical methods, and combination with the CD spectroscopy and Marfey's method. In the prelimi- nary assays, Compound 1 showed cytotoxicity against human embryonic kidney 293 cell line with IC50 value 35.3 [µmol · L(-1).
authors: Ming-hua
authors: MH
authors: Chen
authors: Ye-xiang
authors: YX
authors: Wu
authors: Yan-ni
authors: YN
authors: Xu
authors: Li-yan
authors: LY
authors: Yu
authors: Bin
authors: B
authors: Hong
authors: Wei
authors: W
authors: Jiang
authors: Shu-yi
authors: SY
authors: Si
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D005285:Fermentation; D056945:Hep G2 Cells; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D015394:Molecular Structure; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*
chemical_list: D006877:Hydroxamic Acids