title: A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis).
abstract: The accessory gland (AG) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some AG proteins bind to the spermatozoa and affect its function and properties. Here we report the first comprehensive catalog of the AG secreted fluid during the mature phase of the Chinese mitten crab (Eriocheir sinensis). AG proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Altogether, the mass spectra of 1173 peptides were detected (1067 without decoy and contaminants) which allowed for the identification of 486 different proteins annotated upon the NCBI database (http://www.ncbi.nlm.nih.gov/) and our transcritptome dataset. The mass spectrometry proteomics data have been deposited at the ProteomeXchange with identifier PXD000700. An extensive description of the AG proteome will help provide the basis for a better understanding of a number of reproductive mechanisms, including potentially spermatophore breakdown, dynamic functional and morphological changes in sperm cells and sperm acrosin enzyme vitality. Thus, the comprehensive catalog of proteins presented here can serve as a valuable reference for future studies of sperm maturation and regulatory mechanisms involved in crustacean reproduction.
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Lin
authors: L
authors: He
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Qing
authors: Q
authors: Li
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Lihua
authors: L
authors: Liu
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Yuanli
authors: Y
authors: Wang
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Jing
authors: J
authors: Xie
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Hongdan
authors: H
authors: Yang
authors: School of Life Science, East China Normal University, Shanghai 200241, China.
authors: Qun
authors: Q
authors: Wang
mesh_terms: D000818:Animals; D003445:Crustacea / Q000235:genetics / Q000378:metabolism*; D030562:Databases, Protein; D063990:Gene Ontology; D008297:Male; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012098:Reproduction
chemical_list: D020543:Proteome