title: Molecular and histological characterization of bovine papillomavirus in North West Italy.
abstract: Bovine papillomaviruses (BPVs) are group of worldwide-spread DNA virus that infect primarily cattle determining diseases of considerable economic relevance. Recently, research on BPVs, received a great impulse owing to the development of specific biomolecular analysis, mostly based on L1 gene sequencing, that resulted in the identification of new viral types. This work is aimed at the identification and molecular and histopathological characterization of BPVs circulating in North West Italy, one of the main national cattle breeding areas. In this study, 71 bioptic specimens were submitted both to histological examination and to PCR and sequencing analysis. Histopathology revealed various lesion types; however, no connections were demonstrated between involved viral types and histopathological findings. BPV DNA was demonstrated in all the analyzed samples and several viral types were detected. Particularly, molecular investigations revealed a broad diffusion of highly pathogenic BPV1 and 2 Deltapapillomavirus and presence of BPV3 and 9 Xipapillomavirus. Two cases of co-infection were also demonstrated. Phylogenetic analysis revealed presence of different clusters and therefore a noteworthy genetic variety among the analyzed viral types. This study provides information on the main BPVs types in North West Italy and our results demonstrate the complexity of viral epidemiology which is characterized by circulation of multiple viral types even inside single herds. Knowledge of the prevalence and of the variety of BPVs is a milestone for the development of appropriate prophylactic and therapeutic measures.
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Anna
authors: A
authors: Grindatto
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Giuseppina
authors: G
authors: Ferraro
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Katia
authors: K
authors: Varello
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Maria Ines
authors: MI
authors: Crescio
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Ilaria
authors: I
authors: Miceli
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Elena
authors: E
authors: Bozzetta
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors: Maria
authors: M
authors: Goria
authors: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy. Electronic address: Raffaella.Nappi@izsto.it.
authors: Raffaella
authors: R
authors: Nappi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D060085:Coinfection / Q000662:veterinary; D007558:Italy / Q000453:epidemiology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods
keywords: Bovine papillomavirus (BPV); Histological characterization; Molecular characterization; PCR; Papillomatosis