title: [Inhibitory Effect of Curcumin on Proliferation of CD34(+) Acute Myeloid Leukemia Cells and Its Mechanism].
abstract: OBJECTIVE
  To explore the inhibitory effect of curcumin on proliferation of CD34(+) acute myeloid leukemia cells and its mechamism.
  
  
  METHODS
  KG1a and Kasumi-1cell lines were treated with curcumin of different concentrations (0, 40, 60, 80 µmol/L). The effect of curcumin on cell viability and proliferation was detected by trypan blue staining and cell count. The effect of curcumin on distribution of NF-κB P65 subunit was analyzed by immunofluorescence and Western blot.
  
  
  RESULTS
  The curcumin inhibited proliferation of KG1a and Kasumi-1 cells in a dose-dependent manner. Western blotting showed that curcumin led to significant down-regulation of NF-κB P65 nuclear protein expression. Immunofluorescence assay showed that treatment with 40 µmol/L of curcumin for 48h suppressed the nuclear translocation of NF-κB p65 in KG1a and Kasumi-1 cells.
  
  
  CONCLUSIONS
  The curcumin suppresses cell growth of KG1a and Kasumi-1 cells, its mechanism may be related to inhibitory effect of curcumin on NF-κB p65 nucleus protein.
authors: Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; . E-mail: raojia2004126@126.com.
authors: Jia
authors: J
authors: Rao
authors: Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China;
authors: Rong-Yan
authors: RY
authors: Zhang
authors: Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China;
authors: Guo-An
authors: GA
authors: Chen
authors: Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China;
authors: Fei
authors: F
authors: Li
authors: Department of Hematology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China. E-mial: huangrw56@163.com.
authors: Ren-Wei
authors: RW
authors: Huang
mesh_terms: D018952:Antigens, CD34; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003474:Curcumin; D015536:Down-Regulation; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D016328:NF-kappa B; D051996:Transcription Factor RelA
chemical_list: D018952:Antigens, CD34; D016328:NF-kappa B; D051996:Transcription Factor RelA; D003474:Curcumin