title: Quantification of applied dose in irradiated citrus fruits by DNA Comet Assay together with image analysis.
abstract: The experiments were conducted for quantification of applied dose for quarantine control in irradiated citrus fruits. Citrus fruits exposed to doses of 0.1 to 1.5 kGy and analyzed by DNA Comet Assay. Observed comets were evaluated by image analysis. The tail length, tail moment and tail DNA% of comets were used for the interpretation of comets. Irradiated citrus fruits showed the separated tails from the head of the comet by increasing applied doses from 0.1 to 1.5 kGy. The mean tail length and mean tail moment% levels of irradiated citrus fruits at all doses are significantly different (p < 0.01) from control even for the lowest dose at 0.1 kGy. Thus, DNA Comet Assay may be a practical quarantine control method for irradiated citrus fruits since it has been possible to estimate the applied low doses as small as 0.1 kGy when it is combined with image analysis.
authors: Ondokuz Mayis University, Faculty of Veterinary Medicine, 55139 Kurupelit, Samsun, Turkey. Electronic address: nurcanc@omu.edu.tr.
authors: Nurcan
authors: N
authors: Cetinkaya
authors: Turkish Atomic Energy Authority, Sarayköy Nuclear Research and Training Center, Saray Mah., Atom Cad., No: 27, 06983 Ankara, Turkey.
authors: Demet
authors: D
authors: Ercin
authors: Turkish Atomic Energy Authority, Sarayköy Nuclear Research and Training Center, Saray Mah., Atom Cad., No: 27, 06983 Ankara, Turkey.
authors: Sümer
authors: S
authors: Özvatan
authors: Turkish Atomic Energy Authority, Sarayköy Nuclear Research and Training Center, Saray Mah., Atom Cad., No: 27, 06983 Ankara, Turkey.
authors: Yakup
authors: Y
authors: Erel
mesh_terms: D002957:Citrus / Q000235:genetics / Q000528:radiation effects*; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage; D018744:DNA, Plant / Q000235:genetics; D005514:Food Irradiation / Q000379:methods*; D005638:Fruit / Q000235:genetics / Q000528:radiation effects*; D011829:Radiation Dosage; D012639:Seeds / Q000235:genetics / Q000528:radiation effects; D012984:Software
chemical_list: D018744:DNA, Plant
keywords: Citrus fruits; DNA Comet Assay; Image analysis; Irradiation; Quarantine control method