title: Detection of COL III in parchment by amino acid analysis.
abstract: Cultural heritage parchments made from the reticular dermis of animals have been subject to studies of deterioration and conservation by amino acid analysis. The reticular dermis contains a varying mixture of collagen I and III (COL I and III). When dealing with the results of the amino acid analyses, till now the COL III content has not been taken into account. Based on the available amino acid sequences, we present a method for determining the amount of COL III in the reticular dermis of new and historical parchments calculated from the ratio of Ile/Val. We find COL III contents between 7 and 32 % in new parchments and between 0.2 and 40 % in the historical parchments. This is consistent with results in the literature. The varying content of COL III has a significant influence on the uncertainty of the amino acid analysis. Although we have not found a simple correlation between the COL III content and the degree of deterioration, our results show that this question must be taken into consideration in future studies of the chemical and physical deterioration of parchment measured by amino acid analysis and other analytical methods.
authors: School of Conservation, Royal Danish Academy of Fine Arts Schools of Architecture, Design and Conservation, Esplanaden 34, Copenhagen K, Denmark. dvp@kadk.dk.
authors: Dorte V P
authors: DV
authors: Sommer
authors: School of Conservation, Royal Danish Academy of Fine Arts Schools of Architecture, Design and Conservation, Esplanaden 34, Copenhagen K, Denmark.
authors: René
authors: R
authors: Larsen
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D024042:Collagen Type I / Q000737:chemistry; D024061:Collagen Type III / Q000737:chemistry*; D049672:History, 19th Century; D049673:History, 20th Century; D049674:History, 21st Century; D008969:Molecular Sequence Data; D012867:Skin / Q000737:chemistry*; D014956:Writing / Q000266:history*
chemical_list: D000596:Amino Acids; D024042:Collagen Type I; D024061:Collagen Type III
keywords: Amino acid analysis; Collagen I; Collagen III; Cultural heritage; Oxidation; Parchment