title: Wheat Bran Enhances the Cytotoxicity of Immobilized Alcaligenes aquatilis F8 against Microcystis aeruginosa.
abstract: Algicidal bacteria offer a promising option for killing cyanobacteria. Therefore, a new Alcaligenes aquatilis strain F8 was isolated to control Microcystis aeruginosa in this study. The algicidal activity of strain F8 was dependent on the cell density of M. aeruginosa, and the maximal algicidal rate of the free bacterium reached 88.45% within 72 h. With a view to its application to the control of M. aeruginosa in the natural environment, strain F8 was immobilized in sodium alginate beads, but immobilization of the strain decreased its algicidal rate compared to that of the free bacterium. However, addition of wheat bran to the sodium alginate matrix used to immobilize strain F8 not only eliminated the adverse effects of immobilization on the bacteria but also resulted in an 8.83% higher algicidal rate of the immobilized than free bacteria. Exclusion and recovery methods were used to identify key ingredients of wheat bran and gain insight into the mechanism underlying the observed enhancement of algicidal activity. This analysis indicated that certain factors in wheat bran, including vitamins B1, B2, B9, and E were responsible for promoting bacterial growth and thereby improving the algicidal rate of immobilized strain F8. Our findings indicate that wheat bran is able to improve the algicidal efficiency of A. aquatilis strain F8 for killing M. aeruginosa and is a good source of not only carbon and nitrogen but also vitamins for bacteria.
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors: Pengfei
authors: P
authors: Sun
authors: Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, China.
authors: Hui
authors: H
authors: Lin
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors: Guan
authors: G
authors: Wang
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors: Ximing
authors: X
authors: Zhang
authors: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors: Qichun
authors: Q
authors: Zhang
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors: Yuhua
authors: Y
authors: Zhao
mesh_terms: D000421:Alcaligenes / Q000187:drug effects / Q000502:physiology*; D000464:Alginates; D004043:Dietary Fiber / Q000494:pharmacology*; D005068:Eutrophication / Q000187:drug effects; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D046931:Microcystis / Q000187:drug effects / Q000254:growth & development*
chemical_list: D000464:Alginates; D004043:Dietary Fiber; D006603:Hexuronic Acids; D020723:Glucuronic Acid