title: A proteomic map of the unsequenced kala-azar vector Phlebotomus papatasi using cell line.
abstract: The debilitating disease kala-azar or visceral leishmaniasis is caused by the kinetoplastid protozoan parasite Leishmania donovani. The parasite is transmitted by the hematophagous sand fly vector of the genus Phlebotomus in the old world and Lutzomyia in the new world. The predominant Phlebotomine species associated with the transmission of kala-azar are Phlebotomus papatasi and Phlebotomus argentipes. Understanding the molecular interaction of the sand fly and Leishmania, during the development of parasite within the sand fly gut is crucial to the understanding of the parasite life cycle. The complete genome sequences of sand flies (Phlebotomus and Lutzomyia) are currently not available and this hinders identification of proteins in the sand fly vector. The current study utilizes a three frame translated transcriptomic data of P. papatasi in the absence of genomic sequences to analyze the mass spectrometry data of P. papatasi cell line using a proteogenomic approach. Additionally, we have carried out the proteogenomic analysis of P. papatasi by comparative homology-based searches using related sequenced dipteran protein data. This study resulted in the identification of 1313 proteins from P. papatasi based on homology. Our study demonstrates the power of proteogenomic approaches in mapping the proteomes of unsequenced organisms.
authors: Department of Zoology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, India; Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors: Harsh
authors: H
authors: Pawar
authors: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; Manipal University, Madhav Nagar, Manipal 576104, India.
authors: Sandip
authors: S
authors: Chavan
authors: National Centre for Cell Science, Pune 411007, India.
authors: Kiran
authors: K
authors: Mahale
authors: National Centre for Cell Science, Pune 411007, India.
authors: Sweta
authors: S
authors: Khobragade
authors: National Centre for Cell Science, Pune 411007, India.
authors: Aditi
authors: A
authors: Kulkarni
authors: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors: Arun
authors: A
authors: Patil
authors: National Centre for Cell Science, Pune 411007, India.
authors: Deepa
authors: D
authors: Chaphekar
authors: Armed Forces Medical College, Pashan, Pune 411021, India.
authors: Pratyasha
authors: P
authors: Varriar
authors: National Institute of Virology, Microbial Containment Complex, Pashan, Pune 411021, India.
authors: Anakkathil
authors: A
authors: Sudeep
authors: Department of Zoology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, India.
authors: Kalpana
authors: K
authors: Pai
authors: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
authors: T S K
authors: TSK
authors: Prasad
authors: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India. Electronic address: harsha@ibioinformatics.org.
authors: Harsha
authors: H
authors: Gowda
authors: National Centre for Cell Science, Pune 411007, India. Electronic address: patole@nccs.res.in.
authors: Milind S
authors: MS
authors: Patole
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D007303:Insect Vectors / Q000737:chemistry*; D007893:Leishmania donovani / Q000235:genetics; D007898:Leishmaniasis, Visceral / Q000635:transmission*; D008969:Molecular Sequence Data; D010691:Phlebotomus / Q000737:chemistry* / Q000235:genetics / Q000469:parasitology; D040901:Proteomics
keywords: Comparative homology; Comparative proteogenomics; Disease vector; Hematophagous arthropod; Phlebotomus papatasi