title: Spray Freeze-Drying as an Alternative to the Ionic Gelation Method to Produce Chitosan and Alginate Nano-Particles Targeted to the Colon.
abstract: Chitosan and alginate nano-composite (NP) carriers intended for colonic delivery containing prednisolone and inulin were obtained by two processes. Spray freeze-drying using chitosan (SFDC) or alginate (SFDA) was proposed as an alternative to the traditional chitosan-tripolyphosphate platform (CTPP). NPs were fully characterised and assessed for their yield of particles; level of prednisolone and inulin release in phosphate and Krebs buffers; and sensitivity to degradation by lysozyme, bacteria and faecal slurry. NPs based on chitosan showed similar properties (size, structure, viscoelastic behaviour), but those based on SFDC showed a higher mean release of both active ingredients, with similar efficiency of encapsulation and loading capacity for prednisolone but lower for inulin. SFDC was less degraded in the presence of lysozyme and E. coli and was degraded by B. thetaiotaomicron but not by faecal slurry. The results obtained with SFDA were promising because this NP showed good encapsulation parameters for both active ingredients and biological degradability by E. coli and faecal slurry. However, it will be necessary to use alginate derivatives to reduce its solubility and improve its mechanical behaviour.
authors: Departamento de Ciencia de los Alimentos y Tecnología Química, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Independencia, Santiago de Chile, Chile.
authors: Alexander
authors: A
authors: Gamboa
authors: Departamento de Ciencia de los Alimentos y Tecnología Química, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Independencia, Santiago de Chile, Chile.
authors: Valeria
authors: V
authors: Araujo
authors: Departamento de Ciencia de los Alimentos y Tecnología Química, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Independencia, Santiago de Chile, Chile.
authors: Nelson
authors: N
authors: Caro
authors: Departamento de Nutrición, Facultad de Medicina, Universidad de Chile, Santiago de Chile, Chile.
authors: Martin
authors: M
authors: Gotteland
authors: Departamento de Ciencia de los Alimentos y Tecnología Química, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Independencia, Santiago de Chile, Chile.
authors: Lilian
authors: L
authors: Abugoch
authors: Departamento de Ciencia de los Alimentos y Tecnología Química, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Independencia, Santiago de Chile, Chile. Electronic address: ctapia@uchile.cl.
authors: Cristian
authors: C
authors: Tapia
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001439:Bacteroides / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003106:Colon / Q000382:microbiology*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects; D005243:Feces / Q000382:microbiology; D005260:Female; D005612:Freeze Drying / Q000379:methods; D005782:Gels / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007444:Inulin / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011122:Polyphosphates / Q000737:chemistry; D011239:Prednisolone / Q000737:chemistry; D012995:Solubility
chemical_list: D000464:Alginates; D004337:Drug Carriers; D005782:Gels; D006603:Hexuronic Acids; D011122:Polyphosphates; D020723:Glucuronic Acid; D007444:Inulin; D048271:Chitosan; D011239:Prednisolone; C005692:triphosphoric acid
keywords: alginate; biodegradable polymers; chitosan; colonic drug delivery; nanoparticles; spray freeze-drying