title: Improved light-induced cell detachment on rutile TiO₂ nanodot films.
abstract: Anatase TiO2 nanodot films have been found to be able to release cells under light illumination with excellent efficiency and safety. In the present study, we investigated the effects of rutile contents in TiO2 nanodot films on such light induced cell detachment behavior. The results showed that TiO2 nanodot films with different contents of rutile phase have been prepared successfully. The content of rutile phase increased with the increase in calcination temperature. All films possessed good cell adhesion but there was a decrease in cell proliferation with the increasing content of rutile phase. Single cell detachment assay showed that the films with high rutile contents (calcined at 900°C and 1100°C) showed better cell detachment performance. That was ascribed to the changes of the secondary structure of extracellular proteins adsorbed on the nanodot surface after ultraviolet (365 nm, UV365) illumination. In addition, cell sheets detached through UV365 illumination maintained high activity and could be further used in tissue engineering. The present work showed that the existence of rutile phase is helpful in cell detachment behavior and it could be utilized to optimize light-induced cell detachment behavior.
  
  
  CONCLUSIONS
  This work discovers that the presence of rutile phase in TiO2 nanodot films could improve the light-induced cell detachment behavior, although rutile phase is inferior to anatase phase on light induced superhydrophilicity. That strongly supported that the behaviors of adsorbed proteins are crucial in acquiring cell sheet with light illumination. In fact, the state and behavior of adsorbed protein greatly affect the interaction between biomaterials and living cells. Therefore, we consider this work is not only important in harvesting cells or cell sheets through light illumination, but also helpful in further understanding of interaction between biomaterials and cells.
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors: Kui
authors: K
authors: Cheng
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors: Yu
authors: Y
authors: Sun
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors: Hongping
authors: H
authors: Wan
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
authors: Xiaozhao
authors: X
authors: Wang
authors: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China; The Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China. Electronic address: wengwj@zju.edu.cn.
authors: Wenjian
authors: W
authors: Weng
authors: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors: Jun
authors: J
authors: Lin
authors: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China; The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors: Huiming
authors: H
authors: Wang
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology / Q000528:radiation effects*; D002469:Cell Separation / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D008027:Light; D008422:Materials Testing; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D051379:Mice; D041681:NIH 3T3 Cells; D011829:Radiation Dosage; D014025:Titanium / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D008567:Membranes, Artificial; C009495:titanium dioxide; D014025:Titanium
keywords: Cell detachment; Light-induced; Rutile; TiO(2) nanodot films