title: Role of DetR in defence is critical for virulence of Xanthomonas oryzae pv. oryzae.
abstract: Like other bacteria, Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight disease in rice, possesses intracellular signalling systems, known as two-component regulatory systems (TCSs), which regulate pathogenesis and biological processes. Completion of the genome sequences of three Xoo strains has facilitated the functional study of genes, including those of TCSs, but the biological functions of most Xoo TCSs have not yet been uncovered. To identify TCSs involved in Xoo pathogenesis, we generated knockout strains lacking response regulators (RRs, a cytoplasmic signalling component of the TCS) and examined the virulence of the RR knockout strains. This study presents a knockout strain (detR(-) ) lacking the PXO_04659 gene which shows dramatically reduced virulence relative to the wild-type. Our studies to elucidate detR function in Xoo pathogenesis revealed a reduction in extracellular polysaccharide (EPS), intolerance to reactive oxygen species (ROS) and deregulation of iron homeostasis in the detR(-) strain. Moreover, gene expression of regulatory factors, including other RRs and transcription factors (TFs), was altered in the absence of DetR protein, as determined by reverse transcription-polymerase chain reaction (RT-PCR) and/or real-time quantitative RT-PCR analyses. All evidence leads to the conclusion that DetR is essential for Xoo virulence through the regulation of the Xoo defence system including EPS synthesis, ROS detoxification and iron homeostasis, solely or cooperatively with other regulatory factors.
authors: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.|Institute of Crop Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.
authors: Minh-Phuong
authors: MP
authors: Nguyen
authors: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.|Institute of Crop Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.
authors: Jongchan
authors: J
authors: Park
authors: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.
authors: Man-Ho
authors: MH
authors: Cho
authors: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.|Institute of Crop Biotechnology, Kyung Hee University, Yongin, 446-701, South Korea.
authors: Sang-Won
authors: SW
authors: Lee
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D000071677:Histidine Kinase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000187:drug effects / Q000382:microbiology*; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism; D014974:Xanthomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D017262:Siderophores; D037521:Virulence Factors; D006861:Hydrogen Peroxide; D007501:Iron; D002374:Catalase; D000071677:Histidine Kinase
keywords: DetR (Detoxifying Regulator); Xanthomonas oryzae pv. oryzae; reactive oxygen species; response regulator; two-component regulatory system