title: Effects of acrosomal conditions of frozen-thawed spermatozoa on the results of artificial insemination in Japanese Black cattle.
abstract: The purposes of this study were to examine the relationship between male artificial insemination (AI) fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4) in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques were significantly correlated with the conception rates of routine AI, rates of transferable embryos in superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with oocytes.
authors: Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
authors: Kazumi
authors: K
authors: Kishida
authors: Mitsuhiro
authors: M
authors: Sakase
authors: Kenta
authors: K
authors: Minami
authors: Miyuki M
authors: MM
authors: Arai
authors: Reiko
authors: R
authors: Syoji
authors: Namiko
authors: N
authors: Kohama
authors: Takayuki
authors: T
authors: Akiyama
authors: Akio
authors: A
authors: Oka
authors: Hiroshi
authors: H
authors: Harayama
authors: Moriyuki
authors: M
authors: Fukushima
mesh_terms: D000177:Acrosome / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D002417:Cattle / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000502:physiology; D005615:Freezing; D007246:Infertility / Q000662:veterinary; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D037102:Lectins; D008297:Male; D010755:Organophosphates; D019887:Peanut Agglutinin; D011108:Polymers; D011506:Proteins / Q000032:analysis; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D037102:Lectins; D010755:Organophosphates; D019887:Peanut Agglutinin; D011108:Polymers; D011506:Proteins; C542009:tyrosine polyphosphate