title: Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia.
  
  
  RESULTS
  Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881.
  
  
  CONCLUSIONS
  These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.
authors: Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Komaba 1538902, Japan.
authors: Shota
authors: S
authors: Sakaguchi
authors: Institut Agronomique néo-Calédonien (IAC), Diversités biologique et fonctionnelle des écosystèmes terrestres, BP 73, Port Laguerre, 98890 Païta, New Caledonia.
authors: Guillaume
authors: G
authors: Lannuzel
authors: Institut Agronomique néo-Calédonien (IAC), Diversités biologique et fonctionnelle des écosystèmes terrestres, BP 73, Port Laguerre, 98890 Païta, New Caledonia.
authors: Bruno
authors: B
authors: Fogliani
authors: Institut Agronomique néo-Calédonien (IAC), Diversités biologique et fonctionnelle des écosystèmes terrestres, BP 73, Port Laguerre, 98890 Païta, New Caledonia ; SoREco-NC, 57 Route de l'Anse Vata, 98800 Nouméa, New Caledonia.
authors: Adrien S
authors: AS
authors: Wulff
authors: Institut Agronomique néo-Calédonien (IAC), Diversités biologique et fonctionnelle des écosystèmes terrestres, BP 73, Port Laguerre, 98890 Païta, New Caledonia.
authors: Laurent
authors: L
authors: L'Huillier
authors: Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Komaba 1538902, Japan.
authors: Seikan
authors: S
authors: Kurata
authors: Department of Forest Genetics, Forestry, and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 3058687, Japan.
authors: Saneyoshi
authors: S
authors: Ueno
authors: Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan.
authors: Yuji
authors: Y
authors: Isagi
authors: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 3058577, Japan.
authors: Yoshihiko
authors: Y
authors: Tsumura
authors: Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Komaba 1538902, Japan.
authors: Motomi
authors: M
authors: Ito
keywords: Callitris sulcata; Cupressaceae; New Caledonia; microsatellites; sequencing by synthesis