title: Altered Expression of Oxidative Metabolism Related Genes in Cholangiocarcinomas.
abstract: Cholangiocarcinoma (CCA) is a rare but highly fatal cancer for which the molecular mechanisms and diagnostic markers are obscure. We therefore investigated the kinetic expression of isocitrate dehydrogenase-1 (IDH1), isocitrate dehydrogenase-2 (IDH2) and homogentisate 1,2-dioxygenase (HGD) during the tumorigenesis of O. viverrini infection-associated CCA in an animal model, and confirmed down-regulation of expression in human cases of opisthorchiasis-associated CCA through real time PCR. Kinetic expression of HGD, IDH1 and IDH2 in the animal model of O. viverrini infection-induced CCA was correlated with human CCA cases. In the animal model, expression of HGD was decreased at all time points (p<0.01) and expression of both IDH1 and IDH2 was decreased in the CCA group. In human cases, expression of HGD, IDH1 and IDH2 was decreased more than 2 fold in 55 cases (70.5%), 25 cases (32.1%) and 24 cases (30.8%) respectively. The present study suggests that reduction of HGD, IDH1 and IDH2 may be involve in cholangiocarcinoma genesis and may be useful for molecular diagnosis.
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand E-mail : bthida@kku.ac.th; boonmars@yahoo.com.
authors: Ratchadawan
authors: R
authors: Aukkanimart
authors: Thidarut
authors: T
authors: Boonmars
authors: Amornrat
authors: A
authors: Juasook
authors: Pranee
authors: P
authors: Sriraj
authors: Sirintip
authors: S
authors: Boonjaraspinyo
authors: Zhiliang
authors: Z
authors: Wu
authors: Porntip
authors: P
authors: Laummuanwai
authors: Chawalit
authors: C
authors: Pairojkul
authors: Narong
authors: N
authors: Khuntikeo
authors: Panaratana
authors: P
authors: Rattanasuwan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000556:secondary; D000818:Animals; D001650:Bile Duct Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism* / Q000473:pathology; D002291:Carcinoma, Papillary / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000556:secondary; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D018281:Cholangiocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D005500:Follow-Up Studies; D050560:Homogentisate 1,2-Dioxygenase / Q000378:metabolism*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D008875:Middle Aged; D009367:Neoplasm Staging; D009889:Opisthorchiasis / Q000378:metabolism / Q000401:mortality / Q000469:parasitology / Q000473:pathology; D009891:Opisthorchis / Q000502:physiology; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D007521:Isocitrate Dehydrogenase; D050560:Homogentisate 1,2-Dioxygenase