title: Laboratory Colonization of the Blow Flies, Chrysomya megacephala (Diptera: Calliphoridae) and Chrysomya rufifacies (Diptera: Calliphoridae).
abstract: Chrysomya megacephala (F.) and Chrysomya rufifacies (Macquart) were colonized so that larval growth rates could be compared. Colonies were also established to provide insight into the protein needs of adult C. rufifacies and developmental rates of the ensuing larvae. The C. megacephala and C. rufifacies laboratory colonies were reared for five and six generations, respectively, at 28°C. C. megacephala developmental mean rate from egg to adult was 20.4 ± 0.38 d. First-instar larvae emerge in 1.4 ± 0.24 d, second-instar larvae develop in 2.6 ± 0.38 d and third instars occur at 6.3 ± 0.72 d. Development from egg to pupation occurred in 12 ± 1.10 d. C. rufifacies developed at a mean rate of 16.2 ± 0.78 d from egg to adult emergence. Each stage occurred in succession from first-instar larvae 1.1 ± 0.25 d, second-instar larvae developed 2.3 ± 0.25 d later, and the third-instar larvae developed 5.7 ± 0.41 d later. The larvae pupated 10.0 ± 0.57 d after oviposition. Both of these flies can be collected in the wild and easily colonized using conditioned chicken as an oviposition and larval medium. C. megacephala apparently prefers a lower development and maintenance temperature than C. rufifacies, as evidenced by the high pupal mortality. Laboratory-reared C. rufifacies benefited from bloodmeal as a protein supplement to enhance egg production. C. rufifacies larvae were not observed preying on each other and additional larval species were not provided to serve as prey.
authors: Department of Entomology and Nematology, University of Florida, P.O. Box 110620, Gainesville FL 32611. Present address: Texas A&M AgriLife Extension, 1229 N. U.S.Hwy 281, Stephenville, TX 76401. slswiger@ag.tamu.edu.
authors: Sonja Lise
authors: SL
authors: Swiger
authors: United States Department of Agriculture, Center for Medical, Agricultural and Veterinary Entomology, 1600 SW 23rd Dr., Gainesville, FL 32608.
authors: Jerome A
authors: JA
authors: Hogsette
authors: Department of Entomology and Nematology, University of Florida, P.O. Box 110620, Gainesville FL 32611.
authors: Jerry F
authors: JF
authors: Butler
mesh_terms: D000818:Animals; D004175:Diptera / Q000254:growth & development / Q000502:physiology*; D005260:Female; D044707:Forensic Sciences; D007814:Larva / Q000254:growth & development; D008297:Male; D010058:Oviposition; D011679:Pupa / Q000254:growth & development; D012744:Sex Ratio; D013696:Temperature
keywords: bloodmeal; chicken thigh; forensic entomology; protein source; temperature