title: Characterization of 13 microsatellite markers for Calochortus gunnisonii (Liliaceae) from Illumina MiSeq sequencing.
abstract: OBJECTIVE
  Microsatellite primers were designed for Calochortus gunnisonii (Liliaceae), a montane lily species of the central and southern Rocky Mountains, using next-generation DNA sequencing. The markers will be used to investigate population structure, genetic diversity, and demographic history.
  
  
  RESULTS
  Thirteen polymorphic microsatellite loci were isolated from C. gunnisonii using Illumina MiSeq next-generation DNA sequencing and bioinformatic screening. The mean number of alleles per locus ranged from 4.15 to 5.92 (avg. = 4.97). Observed and expected heterozygosity ranged from 0.077 to 0.871 and 0.213 to 0.782, respectively. The primers were also tested for cross-species amplification value with C. flexuosus, C. nuttallii, C. kennedyi var. kennedyi, and C. subalpinus.
  
  
  CONCLUSIONS
  These primers will be useful for genetic and evolutionary studies across C. gunnisonii's range within the southern and central Rocky Mountains. Furthermore, these markers have proven valuable for cross-species amplifications within Calochortus.
authors: School of Biological Sciences, University of Northern Colorado, 501 20th Street, Greeley, Colorado 80639 USA.
authors: Ryan S
authors: RS
authors: Fuller
authors: School of Biological Sciences, University of Northern Colorado, 501 20th Street, Greeley, Colorado 80639 USA.
authors: Seth
authors: S
authors: Frietze
authors: School of Biological Sciences, University of Northern Colorado, 501 20th Street, Greeley, Colorado 80639 USA.
authors: Mitchell E
authors: ME
authors: McGlaughlin
keywords: Calochortus gunnisonii; Illumina; Liliaceae; MiSeq; microsatellites