title: Profiling of miRNA expression in immune thrombocytopenia patients before and after Qishunbaolier (QSBLE) treatment.
abstract: Immune thrombocytopenia (ITP), also known as idiopathic thrombocytopenic purpura, is an autoimmune disease characterized by low platelet count and increased bleeding tendency. Currently, glucocorticoid and splenectomy are the main therapies for ITP but with obvious side effects including tendency of relapse and risk of internal bleeding. In this study, we report the Mongolian medicine Qishunbaolier (QSBLE) can significantly and efficiently increase platelet count with a low recurrent rate and unnoticeable side effect. We profiled the microRNA (miRNA) expression in the blood sample of ITP patients and identified 44 miRNAs that are differentially expressed in ITP patients before and after QSBLE treatment. Out of these 44 miRNAs, 25 are expressed in control subjects and are downregulated in ITP patients, whereas the treatment with QSBLE restores their expressions to the level of control subjects. This result suggests that abnormal expression of these 25 miRNAs might be connected to the pathogenesis of ITP. Interestingly, 14 of those 44 miNRAs are predicted to target at least once on 31 known IPT associated genes, indicating the possible mechanism of QSBLE on ITP therapy.
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Burenbatu
authors: Department of Chemistry, Delaware State University, Dover, DE 19709, USA.
authors: Mandula
authors: M
authors: Borjigin
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Eerdunduleng
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Wenyan
authors: W
authors: Huo
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Cuiqin
authors: C
authors: Gong
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Hasengaowa
authors: BGI-Shenzhen, Shenzhen, Guangdong 518083, China.
authors: Guiping
authors: G
authors: Zhang
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Longmei
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Ming
authors: M
authors: Li
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Xuemei
authors: X
authors: Zhang
authors: BGI-Shenzhen, Shenzhen, Guangdong 518083, China.
authors: Xiaohui
authors: X
authors: Sun
authors: BGI-Shenzhen, Shenzhen, Guangdong 518083, China.
authors: Jie
authors: J
authors: Yang
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors: Shuanglian
authors: S
authors: Wang
authors: Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors: Narisu
authors: N
authors: Narisu
authors: Department of Developmental Biology, Washington University in St. Louis School of Medicine, St. Louis, MO 63110, USA. Electronic address: yangjianliu@gmail.com.
authors: Yangjian
authors: Y
authors: Liu
authors: Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China; School of Life Science, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China. Electronic address: hh_bai@163.com.
authors: Haihua
authors: H
authors: Bai
mesh_terms: D016022:Case-Control Studies; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D010936:Plant Extracts / Q000627:therapeutic use*; D028321:Plant Preparations / Q000627:therapeutic use*; D010976:Platelet Count; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy* / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: D004365:Drugs, Chinese Herbal; D005819:Genetic Markers; D035683:MicroRNAs; D010936:Plant Extracts; D028321:Plant Preparations; C000611993:qishunbaolier
keywords: Immune thrombocytopenia; Mongolian medicine; Qishunbaolier treatment; microRNA expression