title: Novel plasma microRNA biomarkers for the identification of colitis-associated carcinoma.
abstract: BACKGROUND
  Ulcerative colitis is an established risk factor for colorectal cancer, also known as colitis-associated cancer. Existing colonoscopic-based surveillance has many disadvantages, so a new accurate, efficient, cost-effective screening test is needed. MicroRNAs (miRNAs) regulate gene expression and are dysregulated in a range of diseases, including ulcerative colitis and colorectal cancer. This study aimed to establish the miRNAs associated with colitis-associated cancer.
  
  
  METHODS
  Blood samples were collected from 45 adult patients undergoing colonoscopic screening for ulcerative colitis at the Leicester General Hospital, Leicester, UK. Pool A and B TaqMan Array 384-well cards were used to quantify the expression of 754 miRNAs in the circulating plasma. 28 high priority miRNA candidates showing abnormal expression were validated with real-time quantitative PCR.
  
  
  RESULTS
  Patients were allocated to three disease groups (ulcerative colitis, n=37; dysplasia, n=2; colitis-associated cancer, n=6). Analysis of variance was used to assess differences between the groups. miR-375 was significantly upregulated in the colitis-associated cancer cohort (p=0·0061) compared with active ulcerative colitis. Combining several miRNAs in a panel increased the capacity of the test to distinguish between colitis-associated cancer and different ulcerative colitis activity states.
  
  
  CONCLUSIONS
  Our study suggests that miRNAs have the potential to act as blood-based biomarkers to monitor the activity and progression of disease in patients with ulcerative colitis.
  
  
  BACKGROUND
  Royal College of Surgeons of England.
authors: Department of Cancer Studies, University of Leicester, Leicester, UK. Electronic address: maleene@doctors.org.uk.
authors: Maleene
authors: M
authors: Patel
authors: Department of Cancer Studies, University of Leicester, Leicester, UK.
authors: Ajay
authors: A
authors: Verma
authors: Department of Cancer Studies, University of Leicester, Leicester, UK.
authors: Imran
authors: I
authors: Aslam
authors: Department of Cancer Studies, University of Leicester, Leicester, UK.
authors: Howard
authors: H
authors: Pringle
authors: Department of Cancer Studies, University of Leicester, Leicester, UK.
authors: Baljit
authors: B
authors: Singh