title: Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring.
abstract: In the present work new highly biocompatible nanovesicles were developed using polyanion sodium hyaluronate to form polymer immobilized vesicles, so called hyalurosomes. Curcumin, at high concentration was loaded into hyalurosomes and physico-chemical properties and in vitro/in vivo performances of the formulations were compared to those of liposomes having the same lipid and drug content. Vesicles were prepared by direct addition of dispersion containing the polysaccharide sodium hyaluronate and the polyphenol curcumin to a commercial mixture of soy phospholipids, thus avoiding the use of organic solvents. An extensive study was carried out on the physico-chemical features and properties of curcumin-loaded hyalurosomes and liposomes. Cryogenic transmission electron microscopy and small-angle X-ray scattering showed that vesicles were spherical, uni- or oligolamellar and small in size (112-220 nm). The in vitro percutaneous curcumin delivery studies on intact skin showed an improved ability of hyalurosomes to favour a fast drug deposition in the whole skin. Hyalurosomes as well as liposomes were biocompatible, protected in vitro human keratinocytes from oxidative stress damages and promoted tissue remodelling through cellular proliferation and migration. Moreover, in vivo tests underlined a good effectiveness of curcumin-loaded hyalurosomes to counteract 12-O-tetradecanoilphorbol (TPA)-produced inflammation and injuries, diminishing oedema formation, myeloperoxydase activity and providing an extensive skin reepithelization. Thanks to the one-step and environmentally-friendly preparation method, component biocompatibility and safety, good in vitro and in vivo performances, the hyalurosomes appear as promising nanocarriers for cosmetic and pharmaceutical applications.
authors: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy.
authors: M L
authors: ML
authors: Manca
authors: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy.
authors: I
authors: I
authors: Castangia
authors: Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Centro Mixto Universidad Politécnica de Valencia-Universidad de Valencia, Spain.
authors: M
authors: M
authors: Zaru
authors: Icnoderm Srl, Sardegna Ricerche Ed.5, Pula, Cagliari, 09010, Italy; Dept. of Pharmacy and Pharmaceutical Technology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100-Burjassot, Valencia, Spain.
authors: A
authors: A
authors: Nácher
authors: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy.
authors: D
authors: D
authors: Valenti
authors: Nanomalaria Unit, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain; Barcelona Institute for Global Health (ISGlobal, Hospital Clínic-Universitat de Barcelona), Spain; Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Spain.
authors: X
authors: X
authors: Fernàndez-Busquets
authors: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy.
authors: A M
authors: AM
authors: Fadda
authors: Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy. Electronic address: manconi@unica.it.
authors: M
authors: M
authors: Manconi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003872:Dermatitis / Q000517:prevention & control*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012867:Skin / Q000187:drug effects*; D013552:Swine; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D006820:Hyaluronic Acid; D003474:Curcumin
keywords: Cell oxidative stress; Hyaluronic acid/Hyaluronan; Phospholipid vesicles; Polyphenols; Skin inflammation; Wound healing