title: MiR-378b Promotes Differentiation of Keratinocytes through NKX3.1.
abstract: MicroRNA (miRNA) is a kind of short non-coding RNA, involved in various cellular processes. During keratinocyte differentiation, miRNAs act as important regulators. In this study, we demonstrated by microarray assay that the expression of miR-378b significantly increased during keratinocytes differentiation. Our findings showed that miR-378b could inhibit proliferation, migration and differentiation in keratinocytes. Luciferase reporter assays showed that miR-378b directly target NKX3.1. Silencing of NKX3.1 could coincide with the effects of miR-24 overexpression. In conclusion, our results demonstrate miR-378b promote keratinocytes differentiation by targeting NKX3.1. Manipulation of miR-378b may afford a new strategy to clinic treatment of skin injury and repair.
authors: Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
authors: Xi-Liang
authors: XL
authors: Wang
authors: Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
authors: Tao
authors: T
authors: Zhang
authors: Department of Anus and Intestine Surgery, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China.
authors: Jing
authors: J
authors: Wang
authors: Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
authors: Dian-Bao
authors: DB
authors: Zhang
authors: Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
authors: Feng
authors: F
authors: Zhao
authors: Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
authors: Xue-Wen
authors: XW
authors: Lin
authors: Department of Blood Transfusion, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors: Zhe
authors: Z
authors: Wang
authors: Department of General Practice, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China.
authors: Ping
authors: P
authors: Shi
authors: Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
authors: Xi-Ning
authors: XN
authors: Pang
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; C526012:MIRN378 microRNA, human; D035683:MicroRNAs; C491178:NKX3-1 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors