title: NEURONAL DEVELOPMENT. Glycerophospholipid regulation of modality-specific sensory axon guidance in the spinal cord.
abstract: Glycerophospholipids, the structural components of cell membranes, have not been considered to be spatial cues for intercellular signaling because of their ubiquitous distribution. We identified lyso-phosphatidyl-β-D-glucoside (LysoPtdGlc), a hydrophilic glycerophospholipid, and demonstrated its role in modality-specific repulsive guidance of spinal cord sensory axons. LysoPtdGlc is locally synthesized and released by radial glia in a patterned spatial distribution to regulate the targeting of nociceptive but not proprioceptive central axon projections. Library screening identified the G protein-coupled receptor GPR55 as a high-affinity receptor for LysoPtdGlc, and GPR55 deletion or LysoPtdGlc loss of function in vivo caused the misallocation of nociceptive axons into proprioceptive zones. These findings show that LysoPtdGlc/GPR55 is a lipid-based signaling system in glia-neuron communication for neural development.
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Adam T
authors: AT
authors: Guy
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Yasuko
authors: Y
authors: Nagatsuka
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Noriko
authors: N
authors: Ooashi
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Mariko
authors: M
authors: Inoue
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Asuka
authors: A
authors: Nakata
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Lipid Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Peter
authors: P
authors: Greimel
authors: Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Sendai, Miyagi 980-8578, Japan. Japan Science and Technology Agency, Precursory Research for Embryonic Science and Technology (PRESTO), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors: Asuka
authors: A
authors: Inoue
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Takuji
authors: T
authors: Nabetani
authors: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
authors: Akiho
authors: A
authors: Murayama
authors: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
authors: Kunihiro
authors: K
authors: Ohta
authors: Synthetic Cellular Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors: Yukishige
authors: Y
authors: Ito
authors: Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Sendai, Miyagi 980-8578, Japan. Japan Agency for Medical Research and Development, Core Research for Evolutional Science and Technology (AMED-CREST), 1-7-1 Otemachi, Chiyoda, Tokyo 100-0004, Japan.
authors: Junken
authors: J
authors: Aoki
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kamiguchi@brain.riken.jp hirabaya@riken.jp.
authors: Yoshio
authors: Y
authors: Hirabayashi
authors: RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kamiguchi@brain.riken.jp hirabaya@riken.jp.
authors: Hiroyuki
authors: H
authors: Kamiguchi
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D002642:Chick Embryo; D018920:Coculture Techniques; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology; D055786:Gene Knockout Techniques; D020404:Glycerophospholipids / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D006017:Glycolipids / Q000032:analysis / Q000502:physiology*; D051379:Mice; D020932:Nerve Growth Factor / Q000494:pharmacology; D009457:Neuroglia / Q000502:physiology*; D009619:Nociceptors / Q000502:physiology*; D020917:Receptor, trkA / Q000378:metabolism; D020812:Receptor, trkC / Q000378:metabolism; D043882:Receptors, Cannabinoid / Q000235:genetics / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology*; D046509:Tissue Culture Techniques
chemical_list: C526692:GPR55 protein, mouse; D020404:Glycerophospholipids; D006017:Glycolipids; D043882:Receptors, Cannabinoid; C429823:phosphatidylglucose; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020812:Receptor, trkC