title: Pericardial fluid of cardiac patients elicits arterial constriction: role of endothelin-1.
abstract: Recently, several vasoactive molecules have been found in pericardial fluid (PF). Thus, we hypothesized that in coronary artery disease due to ischemia or ischemia-reperfusion, the level of vasoconstrictors, mainly endothelin-1 (ET-1), increases in PF, which can increase the vasomotor tone of arteries. Experiments were performed using an isometric myograph. Vasomotor effects of PF from patients undergoing coronary artery bypass graft (PFCABG, n = 14) or valve replacement (PFVR, n = 7) surgery were examined in isolated rat carotid arteries (N = 14; n = 26). Vasomotor responses to KCl (40 or 60 mmol/L) were also tested. The selective endothelin A receptor antagonist BQ123 (10(-6) mol/L) was used to elucidate the role of ET-1. Both the first and the second additions of KCl elicited increases in the isometric force of the isolated arteries (KCl1, 6.1 ± 0.2 mN; KCl2, 6.5 ± 0.9 mN). PFCABG and PFVR elicited substantial increases in the isometric force of arteries (PFCABG, 3.1 ± 0.7 mN; PFVR, 3.0 ± 0.9 mN; p > 0.05). The presence of the selective endothelin A receptor blocker significantly reduced arterial contractions to PFCABG (before BQ123, 2.6 ± 0.5 mN vs. after BQ123, 0.8 ± 0.1 mN; p < 0.05). This study is the first to demonstrate that PFs of patients elicit substantial arterial constrictions, which is mediated primarily by ET-1. Interfering with the vasoconstrictor action of PF could be a potential therapeutic target to improve coronary blood flow in cardiac patients.
authors: a University of Pecs, Medical School, Department of Pathophysiology and Gerontology and Szentagothai Research Centre, Pecs, Hungary.
authors: Zoltan
authors: Z
authors: Nemeth
authors: b University of Pecs, Medical School, Heart Institute, Pecs, Hungary.
authors: Attila
authors: A
authors: Cziraki
authors: b University of Pecs, Medical School, Heart Institute, Pecs, Hungary.
authors: Sandor
authors: S
authors: Szabados
authors: b University of Pecs, Medical School, Heart Institute, Pecs, Hungary.
authors: Ivan
authors: I
authors: Horvath
authors: a University of Pecs, Medical School, Department of Pathophysiology and Gerontology and Szentagothai Research Centre, Pecs, Hungary.|c University of Physical Education, Institute of Natural Sciences, Budapest, Hungary.|d Department of Physiology, New York Medical College, Valhalla, NY 10595, USA.
authors: Akos
authors: A
authors: Koller
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000502:physiology*; D003324:Coronary Artery Disease / Q000601:surgery; D065128:Endothelin Receptor Antagonists / Q000494:pharmacology; D019332:Endothelin-1 / Q000032:analysis / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010456:Peptides, Cyclic / Q000494:pharmacology; D000069236:Pericardial Fluid / Q000737:chemistry* / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*
chemical_list: D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D010456:Peptides, Cyclic; D011189:Potassium Chloride; C072247:cyclo(Trp-Asp-Pro-Val-Leu)
keywords: ET-1 receptor; contraction isométrique; coronary bypass; isometric contraction; liquide péricardique; pericardial fluid; pontage coronarien; remplacement valvulaire; récepteur de l’ET-1; valve replacement