title: MicroRNA-10b promotes migration and invasion through Hoxd10 in human gastric cancer.
abstract: BACKGROUND
  This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.
  
  
  METHODS
  The effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated.
  
  
  RESULTS
  miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis.
  
  
  CONCLUSIONS
  miR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.
authors: Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, People's Republic of China. lywyy1979@126.Com.
authors: Yuan-Yu
authors: YY
authors: Wang
authors: Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou, 310014, Zhejiang, People's Republic of China. liydeshy@163.com.
authors: Li
authors: L
authors: Li
authors: Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, People's Republic of China. zaiyuanye@163.com.
authors: Zai-Yuan
authors: ZY
authors: Ye
authors: Departments of Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, Zhejiang, People's Republic of China. zhaozhongsheng1950@163.com.
authors: Zhong-Sheng
authors: ZS
authors: Zhao
authors: Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, People's Republic of China. zlongyan@126.Com.
authors: Zhi-Long
authors: ZL
authors: Yan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000401:mortality / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D018398:Homeodomain Proteins; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D014157:Transcription Factors; C072064:HOXD10 protein, human