title: Impact of Renal Transplantation and Nephrectomy on Urinary Soluble Klotho Protein.
abstract: BACKGROUND
  Klotho is a single-pass transmembrane protein predominantly expressed in the kidneys. The soluble form of klotho has been shown to participate in various pathophysiological activities. However, information regarding the kinetics of soluble klotho remains limited. We herein assessed serial changes in the amounts of 24-hour urinary excreted soluble klotho among renal transplant recipients and concomitant living donors before and after transplantation.
  
  
  METHODS
  A total of 15 recipients and donors were included in the current study, and the amounts of urinary soluble klotho were quantified using a sandwich enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Urine samples were available in 6 of the 15 recipients prior to the procedure. The amounts of urinary klotho in these 6 recipients and overall living donors at the baseline were 58.6 ng/day (IR: 29.3-142) and 698.8 ng/day (IR: 62.3-1619.5), respectively. Those in the recipients on postoperative day 2 (median 522.3 ng/day; IR 337.1-1168.5, P < .05) and day 5 (median 723.2 ng/day; IR 254.7-1238.6, P < .05) were significantly higher than the baseline values. Among the living donors, only a transient increase was observed in the amounts of urinary klotho on postoperative day 2.
  
  
  CONCLUSIONS
  The current data regarding the urinary soluble klotho in recipients support the hypothesis that the kidney is a major source of urinary soluble klotho among the numerous components of the urinary tract. In living donors, the complex nature of events associated with acute reductions in the renal mass may modulate the release of soluble klotho from the kidneys into the urine.
authors: Division of Renal Surgery and Transplantation, Department of Urology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: T
authors: T
authors: Kimura
authors: Division of Nephrology, Department of Internal Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan. Electronic address: tetsu-a@jichi.ac.jp.
authors: T
authors: T
authors: Akimoto
authors: Division of Nephrology, Department of Internal Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: Y
authors: Y
authors: Watanabe
authors: Division of Renal Surgery and Transplantation, Department of Urology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: A
authors: A
authors: Kurosawa
authors: Division of Renal Surgery and Transplantation, Department of Urology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: K
authors: K
authors: Nanmoku
authors: Division of Nephrology, Department of Internal Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: S
authors: S
authors: Muto
authors: Division of Nephrology, Department of Internal Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: E
authors: E
authors: Kusano
authors: Division of Renal Surgery and Transplantation, Department of Urology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: T
authors: T
authors: Yagisawa
authors: Division of Nephrology, Department of Internal Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors: D
authors: D
authors: Nagata
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005966:Glucuronidase / Q000652:urine*; D006084:Graft Rejection / Q000652:urine*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000601:surgery* / Q000652:urine; D016030:Kidney Transplantation; D000090265:Klotho Proteins; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy; D011184:Postoperative Period; D066027:Transplant Recipients
chemical_list: D005966:Glucuronidase; D000090265:Klotho Proteins