title: Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells.
abstract: We demonstrate that dual release of bone morphogenic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) by catechol-functionalized adhesive polymer nanoparticles on microgrooved titanium (Ti) surface enhances in vitro osteoblastic differentiation of human mesenchymal stem cells (MSCs). The nanoparticles consisted of three distinct domains, surface Ti-adhesive catechol groups, anionic poly(L-aspartic acid) (PAsp) shells, and hydrophobic poly(L-phenylalanine) (PPhe) cores. The immobilization of the adhesive nanoparticles onto microgrooved Ti surface was verified using various surface analytical tools, such as field-emission scanning electron microscopy (Fe-SEM), X-ray photoelectron spectroscopy (XPS), contact angle measurement. The nanoparticles were immobilized both on the groove bottom surface and the ridge top surface with a similar anchoring density. A fluorescence microscope visualized that BMP-2 and IGF-1 of positive charges were efficiently loaded onto the negatively charged PAsp shells of immobilized nanoparticles. We confirmed the enhanced osteoblastic differentiation of MSCs by presenting the expression results of major osteoblast marker genes and proteins. In addition, overall significant correlations between the experimental results verified the validity of our study. The proposed combined surface of microgrooves and growth factor-releasing nanoparticles can be used as a strong osteogenic promoter on various biomaterial surfaces.
authors: Department of Biomaterials & Prosthodontics, Kyung Hee University Hospital at Gangdong, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 892 Dongnam-ro, Gangdong-gu, Seoul 134-727, Republic of Korea.
authors: Suk Won
authors: SW
authors: Lee
authors: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors: Hong Jae
authors: HJ
authors: Lee
authors: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors: Jae Won
authors: JW
authors: Lee
authors: Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, Seoul 134-727, Republic of Korea.
authors: Kyung-Hee
authors: KH
authors: Kim
authors: Green Ceramics Division, Korea Institute of Ceramic Engineering and Technology, 77 10-gil, Digital-ro, Geumcheon-gu, Seoul 153-801, Republic of Korea.
authors: Jong-Ho
authors: JH
authors: Kang
authors: Green Ceramics Division, Korea Institute of Ceramic Engineering and Technology, 77 10-gil, Digital-ro, Geumcheon-gu, Seoul 153-801, Republic of Korea.
authors: Myung Hyun
authors: MH
authors: Lee
authors: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea. Electronic address: schlee@khu.ac.kr.
authors: Sang Cheon
authors: SC
authors: Lee
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D053758:Nanoparticles; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D055396:Bone Morphogenetic Protein 2; D010455:Peptides; C017645:polyaspartate; C012001:polyphenylalanine; D007334:Insulin-Like Growth Factor I; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Adhesive nanoparticle; Bone morphogenic protein-2; Insulin-like growth factor-1; Microgrooved titanium; Osteoblastic differentiation