title: Purification of Thymocyte and T Cell Subsets.
abstract: Many analytical or cell culture procedures require homogeneous starting cell populations that cannot be obtained directly from organ dissection. Here, we describe two enrichment procedures to achieve this goal and discuss their respective advantages in specific experimental contexts. Notes in this chapter include some tips on how to determine the appropriate level of purity (see Note 1 ).
authors: Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
authors: Andrea C
authors: AC
authors: Carpenter
authors: Jong Kyong
authors: JK
authors: Kim
authors: Rémy
authors: R
authors: Bosselut
mesh_terms: D005434:Flow Cytometry / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods*; D016130:Immunophenotyping; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism*; D060168:Thymocytes / Q000166:cytology* / Q000378:metabolism*