title: Salidroside attenuates lipopolysaccharide (LPS) induced serum cytokines and depressive-like behavior in mice.
abstract: The aim of the study was to investigate the effects and possible underlying mechanism of salidroside (Sal) on lipopolysaccharide (LPS)-induced depression-like behavior in mice. Sal (12 mg/kg and 24 mg/kg) and fluoxetine (20 mg/kg) were administered intragastrically once daily for 5 days. At the 5th day, LPS (0.5 mg/kg) was injected intraperitoneally 30 min after drug administration. Levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in serum were measured by ELISA. Levels of neurotransmitters like norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in the prefrontal cortex were detected by HPLC-MS. Further, brain-derived neurotrophic factor (BDNF), tropomyosin-related kinase B (TrkB) and Nuclear factor-κB (NF-κB) in hippocampal was determined by western blot analysis. Our data showed that pretreatment with Sal dramatically attenuated LPS-induced inflammatory response, decrease of NE and 5-HT levels in the prefrontal cortex. In addition, Sal increased expression levels of BNDF and TrkB. These results suggested that Sal may play a neuroprotective role through the BDNF/TrkB signaling pathway.
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China.
authors: Lingpeng
authors: L
authors: Zhu
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China.
authors: Tingting
authors: T
authors: Wei
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China.
authors: Jing
authors: J
authors: Gao
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China.
authors: Xiayun
authors: X
authors: Chang
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China.
authors: He
authors: H
authors: He
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China. Electronic address: miaomingxing25@126.com.
authors: Mingxing
authors: M
authors: Miao
authors: Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China. Electronic address: yantianhuabest@126.com.
authors: Tianhua
authors: T
authors: Yan
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D016207:Cytokines / Q000097:blood*; D003863:Depression / Q000188:drug therapy* / Q000378:metabolism / Q000523:psychology; D004789:Enzyme Activation; D005960:Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D009638:Norepinephrine / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology / Q000627:therapeutic use*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D020813:Receptor, trkB
chemical_list: D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D005960:Glucosides; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D018696:Neuroprotective Agents; D009638:Norepinephrine; D010636:Phenols; D011505:Protein-Tyrosine Kinases; D012701:Serotonin; C064371:Ntrk2 protein, mouse; D020813:Receptor, trkB; C009172:rhodioloside
keywords: BDNF; Depression; Inflammation; Lipopolysaccharide; Salidroside