title: MicroRNA 26a inhibits HMGB1 expression and attenuates cardiac ischemia-reperfusion injury.
abstract: Ischemia reperfusion (IR) injury is a major issue in cardiac transplantation and inflammatory processes play a major role in myocardial IR injury. MicroRNA 26a (Mir-26a) plays important roles in cellular differentiation, cell growth, cell apoptosis and metastasis. Mir-26a has been demonstrated to modulate regulatory T cells expansion and attenuates renal IR injury. However, the role of Mir-26a in the cardiac IR injury has never been investigated. In our study, hearts of C57BL/6 mice were flushed and stored in cold Bretschneider solution for 8 hours and then transplanted into syngeneic recipients. The results demonstrate a crucial role for Mir-26a in inhibiting high mobility group box-1 (HMGB1) expression and attenuating cardiac IR injury. Mir-26a overexpression results in attenuated cardiac IR injury and inhibited HMGB1 expression. Mir-26a also inhibits inflammatory cells infiltration and cytokines expression. Furthermore, the attenuated cardiac IR injury induced by Mir-26a was abrogated by additional administration of recombinant HMGB1 (rHMGB1). In conclusion, Mir-26a plays a protective role in cardiomyocyte IR injury and this is associated with inhibited HMGB1 expression.
authors: Linyi People's Hospital General Internal, PR China.
authors: Li
authors: L
authors: Yao
authors: Linyi People's Hospital Respiratory Medicine, PR China.
authors: Xin
authors: X
authors: Lv
authors: Linyi People's Hospital General Internal, PR China. Electronic address: xhwangsd@126.com.
authors: Xiaohua
authors: X
authors: Wang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016027:Heart Transplantation; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020107:Troponin T / Q000097:blood
chemical_list: D016207:Cytokines; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D035683:MicroRNAs; C543693:Mirn26 microRNA, mouse; D012333:RNA, Messenger; D020107:Troponin T
keywords: Cardiac IR injury; HMGB1; MicroRNA 26a