title: Fatty alcohols production by oleaginous yeast.
abstract: We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2 g/L. In many of the conditions tested, more than 80 % of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7 L bioreactors, over 8 g/L of C(16)-C(18) fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date.
authors: Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain.
authors: Sandy
authors: S
authors: Fillet
authors: Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain.
authors: Jordi
authors: J
authors: Gibert
authors: Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain.
authors: Beatriz
authors: B
authors: Suárez
authors: Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain.
authors: Armando
authors: A
authors: Lara
authors: Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain.
authors: Carmen
authors: C
authors: Ronchel
authors: Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain. jladrio@neolbio.com.
authors: José L
authors: JL
authors: Adrio
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005233:Fatty Alcohols / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D020563:Gammaproteobacteria / Q000201:enzymology / Q000235:genetics; D013395:Sucrose / Q000378:metabolism
chemical_list: D003470:Culture Media; D005233:Fatty Alcohols; D013395:Sucrose; D000445:Aldehyde Oxidoreductases; C021351:hexadecanal dehydrogenase (acylating)
keywords: Fatty acyl-CoA reductase; Fatty alcohols; Metabolic engineering; Rhodosporidium toruloides