title: Ricinus communis L. stem bark extracts regulate ovarian cell functions and secretory activity and their response to Luteinising hormone.
abstract: Ricinus communis L. has ethnopharmacological contraceptive reputation but its stem bark has unexplored mechanisms of action in female reproductive system. In the present study, the effect of methanolic and aqueous extracts from the stem bark of the plant was examined on basic porcine ovarian granulosa cell functions and its response to Luteinising hormone (LH)-the upstream hormonal regulator. Systemic treatment of methanolic and aqueous extracts stimulated cell proliferation (proliferating cell nuclear antigen, PCNA) and also promoted cell apoptosis (caspase-3). Aqueous extract has inverted the stimulatory effect of LH on PCNA but not on caspase-3. Methanolic extract stimulated as well as inhibited progesterone release and stimulated testosterone secretion. Whereas aqueous extract inhibited both steroid releases and suppressed the stimulatory effect of LH on progesterone release and promoted the inhibitory effect of LH on testosterone release. In conclusion, the present study unveils the mechanism of action of R. communis stem bark in in vitro condition. These suggest its possible contraceptive efficacy by exerting its regulatory role over LH and on basic ovarian cell functions and secretion activity.
authors: Department of Animal Genetics and Reproduction, Animal Production Research Centre Nitra, Institute for Genetics and Reproduction of Farm Animals, Luzianky, Slovak Republic.|Department of Life Science and Bioinformatics, Assam University, Silchar, India.
authors: S
authors: S
authors: Nath
authors: Department of Animal Genetics and Reproduction, Animal Production Research Centre Nitra, Institute for Genetics and Reproduction of Farm Animals, Luzianky, Slovak Republic.|Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic.
authors: A
authors: A
authors: Kadasi
authors: Department of Functional Genomics and Bioregulation, Federal Research Centre, Institute of Animal Science, Neustadt, Germany.
authors: R
authors: R
authors: Grossmann
authors: Department of Animal Genetics and Reproduction, Animal Production Research Centre Nitra, Institute for Genetics and Reproduction of Farm Animals, Luzianky, Slovak Republic.|Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovak Republic.
authors: A V
authors: AV
authors: Sirotkin
authors: Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic.
authors: A
authors: A
authors: Kolesarova
authors: Department of Life Science and Bioinformatics, Assam University, Silchar, India.
authors: A D
authors: AD
authors: Talukdar
authors: Department of Life Science and Bioinformatics, Assam University, Silchar, India.
authors: M D
authors: MD
authors: Choudhury
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003271:Contraceptive Agents, Female; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D007986:Luteinizing Hormone / Q000494:pharmacology*; D000432:Methanol; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012278:Ricinus / Q000737:chemistry*; D034421:Sus scrofa / Q000502:physiology*; D013739:Testosterone / Q000378:metabolism; D014867:Water
chemical_list: D003271:Contraceptive Agents, Female; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D014867:Water; D013739:Testosterone; D011374:Progesterone; D007986:Luteinizing Hormone; D053148:Caspase 3; D000432:Methanol