title: Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells.
abstract: Microfluidic devices employ submillimeter length scale control of flow to achieve high-resolution spatial and temporal control over the microenvironment, providing powerful tools to elucidate mechanisms of human pluripotent stem cell (hPSC) regulation and to elicit desired hPSC fates. In addition, microfluidics allow control of paracrine and juxtracrine signaling, thereby enabling fabrication of microphysiological systems comprised of multiple cell types organized into organs-on-a-chip. Microfluidic cell culture systems can also be integrated with actuators and sensors, permitting construction of high-density arrays of cell-based biosensors for screening applications. This review describes recent advances in using microfluidics to understand mechanisms by which the microenvironment regulates hPSC fates and applications of microfluidics to realize the potential of hPSCs for in vitro modeling and screening applications.
authors: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
authors: Tongcheng
authors: T
authors: Qian
authors: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
authors: Eric V
authors: EV
authors: Shusta
authors: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA. Electronic address: sppalecek@wisc.edu.
authors: Sean P
authors: SP
authors: Palecek
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics*; D060833:Cellular Microenvironment / Q000235:genetics; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D019899:Paracrine Communication / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology*