title: Pre-organized Guide RNA in the Cas9 Complex Is Ready for the Selection of Target Double-Stranded DNA.
abstract: The 3 D structure of Cas9 bound with sgRNA was solved by X-ray crystal-structure analysis. The conformation change in SpyCas9 upon binding to sgRNA changes Cas9 to target-DNA-recognition mode in which seed segments in sgRNA are pre-ordered in an A-type conformation.
authors: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.|Division of Green Conversion, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
authors: Yukiko
authors: Y
authors: Kamiya
authors: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. asanuma@nubio.nagoya-u.ac.jp.
authors: Hiroyuki
authors: H
authors: Asanuma
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D020868:Gene Silencing; D009690:Nucleic Acid Conformation; D017434:Protein Structure, Tertiary; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems; D004247:DNA
keywords: CRISPR/Cas; DNA silencing; crystal structures; genome editing; seed RNA