title: Biosynthesis of 1,2-dihydroxydibenzofuran by magnetically immobilized cells of Escherichia coli expressing phenol hydroxylase in liquid-liquid biphasic systems.
abstract: Escherichia coli cells expressing phenol hydroxylase (designated as PHIND) were used to biosynthesize 1,2-dihydroxydibenzofuran (1,2-dihydroxyDBF) from dibenzofuran (DBF). The pathway of DBF biotransformation by strain PHIND was proposed, in which DBF was initially monohydroxylated at C-1 and C-4 positions to produce 1- and 4-hydroxyDBF, then underwent successive hydroxylation to yield 1,2- and 3,4-dihydroxyDBF, of which 1,2-dihydroxyDBF was identified for the first time. Magnetically immobilized cells of strain PHIND in biphasic systems with dodecane as the solvent presented highest biosynthesis activity for 1,2-dihydroxyDBF, which was a 6.5-fold improvement compared to biosynthesis in aqueous system. The recycling experiments demonstrated that magnetically immobilized cells exhibited higher biosynthesis activity for 1,2-dihydroxyDBF than that by nonmagnetically immobilized cells during five cycles in biphasic systems. These works support the development of an efficient biosynthesis process using magnetically immobilized cells in biphasic systems and provide a promising technique for improving the productivity in 1,2-dihydroxyDBF biosynthesis.
authors: School of Life Science, Liaoning Normal University, Dalian 116081, China.
authors: Shengnan
authors: S
authors: Shi
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Yuanyuan
authors: Y
authors: Qu
authors: School of Life Science, Liaoning Normal University, Dalian 116081, China.
authors: Liang
authors: L
authors: Tan
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: hit_dlut@163.com.
authors: Fang
authors: F
authors: Ma
mesh_terms: D019149:Bioreactors; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D059346:Magnets; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase
keywords: 1,2-Dihydroxydibenzofuran; Biosynthesis; Liquid–liquid biphasic systems; Magnetic immobilization