title: Equine Cutaneous Mast Cell Tumours Exhibit Variable Differentiation, Proliferation Activity and KIT Expression.
abstract: Equine cutaneous mast cell tumours (CMCTs) are generally considered to be benign skin lesions, although recurrent and multicentric tumours have been described. For canine CMCTs, grading and prognostic approaches are well established and aberrant KIT expression as well as high proliferation indices are associated with poor outcome. However, in the case of equine CMCTs, morphological features, proliferative activity and KIT expression pattern have not been assessed or related to biological behaviour, and there is discussion as to whether CMCTs are true neoplastic processes. The present study describes 45 equine CMCTs in terms of their morphology and KIT and PCNA expression by immunohistochemistry. KIT expression was classified as membranous (I), cytoplasmic and focally stippled (II) or diffuse cytoplasmic (III). A large proportion of the tumours were multinodular or diffuse dermal infiltrates of mast cells with mild anisokaryosis, a low proliferative rate and a dominance of KIT pattern I, representing well-differentiated CMCTs. In approximately one third of the cases, the mast cells exhibited more infiltrative growth, moderate to marked anisokaryosis and a higher degree of proliferation. These were classified as poorly differentiated CMCTs and exhibited only KIT patterns II and III. These findings indicate that there is a subgroup of poorly differentiated equine CMCTs, in which there is an association between aberrant KIT expression, high proliferative rate and potential aggressive behaviour, all features that confirm at least the poorly differentiated CMCT as a true neoplastic processes.
authors: Division of Veterinary Pathology, School of Veterinary Science, University of Liverpool, Leahurst Campus, Chester High Road, Neston, UK. Electronic address: l.ressel@liverpool.ac.uk.
authors: L
authors: L
authors: Ressel
authors: Division of Veterinary Pathology, School of Veterinary Science, University of Liverpool, Leahurst Campus, Chester High Road, Neston, UK.
authors: S
authors: S
authors: Ward
authors: Division of Veterinary Pathology, School of Veterinary Science, University of Liverpool, Leahurst Campus, Chester High Road, Neston, UK; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 268, Zurich, Switzerland.
authors: A
authors: A
authors: Kipar
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006734:Horse Diseases / Q000378:metabolism / Q000473:pathology*; D006736:Horses; D007150:Immunohistochemistry; D008297:Male; D034701:Mastocytosis, Cutaneous / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D019009:Proto-Oncogene Proteins c-kit
keywords: KIT; PCNA; equine; mast cell tumour; mitotic index