title: Nanoliposome-mediated targeting of antibodies to tumors: IVIG antibodies as a model.
abstract: Monoclonal antibodies are routinely used as tools in immunotherapies against solid tumors. However, administration of monoclonal antibodies may cause undesired side effects due to their accumulation in non-targeted organs. Nanoliposomes of less than 200 nm can target antibodies to tumors by enhanced permeation and retention (EPR) mechanisms. To direct monoclonal antibodies to tumors, nanoliposomes encapsulating intravenous immunoglobulin (IVIG) as a model antibody were prepared. The liposomes had average diameters of 100 nm and encapsulation efficiencies of 31 to 46%. They showed less than 10% release in plasma at 37°C up to seven days. The secondary and tertiary structures of liposome-encapsulated antibodies were analyzed by circular dichroism (CD) spectroscopy. The near and far-UV spectra analyses revealed no obvious conformational changes in the structures of the encapsulated antibodies. The biodistribution of free and liposome-encapsulated iodinated antibodies was investigated in mice bearing C-26 colon carcinoma tumors. The accumulation of liposome-encapsulated antibodies in tumors was significantly greater than that of free antibodies due to the EPR effect. The PEGylated liposomes were more efficient in the delivery of antibodies to the tumor site than non-PEGylated liposomes. We conclude that administration of monoclonal antibodies in PEGylated liposomes is more efficient than administration of non-encapsulated monoclonal antibodies for solid tumor immunotherapy.
authors: Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad 919677-3117​, Iran; Student Research Committee, Mashhad University of Medical Sciences, Mashhad 913881-3944, Iran.
authors: Amin Reza
authors: AR
authors: Nikpoor
authors: Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad 919677-3117​, Iran.
authors: Jalil
authors: J
authors: Tavakkol-Afshari
authors: Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad 919677-3117​, Iran; Student Research Committee, Mashhad University of Medical Sciences, Mashhad 913881-3944, Iran.
authors: Zahra
authors: Z
authors: Gholizadeh
authors: Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad 98451-3546, Iran.
authors: Kayvan
authors: K
authors: Sadri
authors: Nuclear Biomolecule Laboratory, Radioisotope Department, Nuclear Science and Technology Research Institute, Tehran 14144-1339, Iran.
authors: Mohammad Hossein
authors: MH
authors: Babaei
authors: Department of Biochemistry and Biophysics, Faculty of sciences, Mashhad Branch, Islamic Azad University, Mashhad 918714-7578, Iran.
authors: Jamshidkhan
authors: J
authors: Chamani
authors: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 91775-1365, Iran.
authors: Ali
authors: A
authors: Badiee
authors: Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran 198571-7443, Iran. Electronic address: jalalia@sbmu.ac.ir.
authors: Seyed Amir
authors: SA
authors: Jalali
authors: Biotechnology Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 91775-1365, Iran. Electronic address: Jafarimr@mums.ac.ir.
authors: Mahmoud Reza
authors: MR
authors: Jaafari
mesh_terms: D000818:Animals; D001682:Biological Availability; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D004355:Drug Stability; D016756:Immunoglobulins, Intravenous / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D007167:Immunotherapy; D008081:Liposomes / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics; D051379:Mice; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D016756:Immunoglobulins, Intravenous; D008081:Liposomes
keywords: Antibodies; Cancer; Chloroform (PubChem CID: 6212); Cholesterol (PubChem CID: 5997); Iodine-125 (PubChem CID: 104800); Liposomes; Octyl-beta-d-glucopyranoside (PubChem CID: 62852); Tissue distribution