title: Towards the Design of 3D Fiber-Deposited Poly(ε-caprolactone)/lron-Doped Hydroxyapatite Nanocomposite Magnetic Scaffolds for Bone Regeneration.
abstract: In the past few years, researchers have focused on the design and development of three-dimensional (3D) advanced scaffolds, which offer significant advantages in terms of cell performance. The introduction of magnetic features into scaffold technology could offer innovative opportunities to control cell populations within 3D microenvironments, with the potential to enhance their use in tissue regeneration or in cell-based analysis. In the present study, 3D fully biodegradable and magnetic nanocomposite scaffolds for bone tissue engineering, consisting of a poly(ε-caprolactone) (PCL) matrix reinforced with iron-doped hydroxyapatite (FeHA) nanoparticles, were designed and manufactured using a rapid prototyping technique. The performances of these novel 3D PCL/FeHA scaffolds were assessed through a combination of theoretical evaluation, experimental in vitro analyses and in vivo testing in a rabbit animal model. The results from mechanical com- pression tests were consistent with FEM simulations. The in vitro results showed that the cell growth in the magnetized scaffolds was 2.2-fold greater than that in non-magnetized ones. In vivo experiments further suggested that, after only 4 weeks, the PCL/FeHA scaffolds were completely filled with newly formed bone, proving a good level of histocompatibility. All of the results suggest that the introduction of magnetic features into biocompatible materials may confer significant advantages in terms of 3D cell assembly.
authors: Roberta
authors: R
authors: De Santis
authors: Alessandro
authors: A
authors: Russo
authors: Antonio
authors: A
authors: Gloria
authors: Ugo
authors: U
authors: D'Amora
authors: Teresa
authors: T
authors: Russo
authors: Silvia
authors: S
authors: Panseri
authors: Monica
authors: M
authors: Sandri
authors: Anna
authors: A
authors: Tampieri
authors: Maurilio
authors: M
authors: Marcacci
authors: Valentin A
authors: VA
authors: Dediu
authors: Colin J
authors: CJ
authors: Wilde
authors: Luigi
authors: L
authors: Ambrosio
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000138:chemical synthesis; D019544:Equipment Failure Analysis; D005264:Femoral Fractures / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D007501:Iron / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010315:Particle Accelerators; D011091:Polyesters / Q000737:chemistry*; D011474:Prosthesis Design; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D018786:Bone Substitutes; D058185:Magnetite Nanoparticles; D011091:Polyesters; C016240:polycaprolactone; D007501:Iron