title: A Comprehensive Proteomics Analysis Reveals a Secretory Path- and Status-Dependent Signature of Exosomes Released from Tumor-Associated Macrophages.
abstract: Exosomes are 30-120 nm-sized membrane vesicles of endocytic origin that are released into the extracellular environment and play roles in cell-cell communication. Tumor-associated macrophages (TAMs) are important constituents of the tumor microenvironment; thus, it is critical to study the features and complex biological functions of TAM-derived exosomes. Here, we constructed a TAM cell model from a mouse macrophage cell line, Ana-1, and performed comparative proteomics on exosomes, exosome-free media, and cells between TAMs and Ana-1. Proteomic analysis between exosome and exosome-free fractions indicated that the functions of exosome dominant proteins were mainly enriched in RNA processing and proteolysis. TAM status dramatically affected the abundances of 20S proteasome subunits and ribosomal proteins in their exosomes. The 20S proteasome activity assay strongly indicated that TAM exosomes possessed higher proteolytic activity. In addition, Ana-1- and TAM-derived exosomes have different RNA profiles, which may result from differential RNA processing proteins. Taken together, our comprehensive proteomics study provides novel views for understanding the complicated roles of macrophage-derived exosomes in the tumor microenvironment.
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors: Yinghui
authors: Y
authors: Zhu
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors: Xianwei
authors: X
authors: Chen
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors: Qingfei
authors: Q
authors: Pan
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors: Yang
authors: Y
authors: Wang
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors: Siyuan
authors: S
authors: Su
authors: Beijing Protein Innovation , Beijing 101318, China.
authors: Cuicui
authors: C
authors: Jiang
authors: Beijing Protein Innovation , Beijing 101318, China.
authors: Yang
authors: Y
authors: Li
authors: Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100021, China.
authors: Ningzhi
authors: N
authors: Xu
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.
authors: Lin
authors: L
authors: Wu
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.
authors: Xiaomin
authors: X
authors: Lou
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100101, China.
authors: Siqi
authors: S
authors: Liu
mesh_terms: D000818:Animals; D002450:Cell Communication; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D057075:Enzyme Assays; D055354:Exosomes / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D058977:Molecular Sequence Annotation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012323:RNA Processing, Post-Transcriptional; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D059016:Tumor Microenvironment / Q000235:genetics
chemical_list: D020543:Proteome; D012269:Ribosomal Proteins; D046988:Proteasome Endopeptidase Complex
keywords: exosome; iTRAQ; proteasome; protein signature; ribosome; tumor-associated macrophage