title: Stress during the gestational period modifies pups' emotionality parameters and favors preference for morphine in adolescent rats.
abstract: Experimental animal studies have shown that early life periods are highly vulnerable to environmental factors, which may exert prolonged impact on HPA axis function and on subsequent neurochemical and behavioral responses in adulthood. Here we evaluated the influence of environmental stressful situations in two different early life stages on stress-related behaviors, and morphine-conditioned place preference (CPP), which is indicative of addiction. While in the gestational stress (Gest-S) dams were exposed to daily sessions of chronic mild stress (CMS) for 2 weeks, in the postnatal stress (post-NS) the offspring were exposed daily to neonatal isolation from postnatal day (PND) 2 to PND 9 for 60 min. Animals exposed to post-NS showed lesser anxiety in different behavioral paradigms (elevated plus maze-EPM and defensive burying test-DBT) as well as increased exploratory behavior (open-field task-OFT), and no preference for morphine in CPP. In contrast, animals exposed to Gest-S showed increased corticosterone plasma levels together with anxiety symptoms and greater preference for morphine following three days of drug withdrawal. Our findings indicate that the gestational period is critical for stress, whose effects may be manifest throughout life. On the other hand, post-NS can trigger neuroadaptations able to overcome emotional consequences of early life. We hypothesized that Gest-S is able to modify responses to opioids along adulthood, which may facilitate development of addiction to these drugs.
authors: Programa de Pós Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Prédio 18, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Luciana Taschetto
authors: LT
authors: Vey
authors: Departamento de Fisiologia e Farmacologia, UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Higor Zuquetto
authors: HZ
authors: Rosa
authors: Programa de Pós Graduação em Farmacologia UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Raquel Cristine Silva
authors: RCS
authors: Barcelos
authors: Programa de Pós Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Prédio 18, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Hecson Jesser
authors: HJ
authors: Segat
authors: Departamento de Fisiologia e Farmacologia, UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Vinícia Garzella
authors: VG
authors: Metz
authors: Programa de Pós Graduação em Farmacologia UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Verônica Tironi
authors: VT
authors: Dias
authors: Programa de Pós Graduação em Farmacologia UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil.
authors: Thiago
authors: T
authors: Duarte
authors: Programa de Pós Graduação em Farmacologia UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil; Lutheran University of Brazil (ULBRA), Santa Maria, Brazil.
authors: Marta M M F
authors: MMMF
authors: Duarte
authors: Programa de Pós Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Prédio 18, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil; Programa de Pós Graduação em Farmacologia UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil; Departamento de Fisiologia e Farmacologia, UFSM, Av. Roraima, 1000, Prédio 21, Cidade Universitária, CEP 97105-900 Santa Maria, RS, Brazil. Electronic address: mariliseeb@yahoo.com.br.
authors: Marilise Escobar
authors: ME
authors: Burger
mesh_terms: D000367:Age Factors; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D003345:Corticosterone / Q000097:blood*; D004644:Emotions / Q000502:physiology*; D005260:Female; D008297:Male; D009021:Morphine Dependence / Q000209:etiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000150:complications* / Q000209:etiology
chemical_list: D003345:Corticosterone
keywords: Addiction; Anxiety; Gestational stress; Morphine; Post-natal stress