title: Continuous propionic acid production with Propionibacterium acidipropionici immobilized in a novel xylan hydrogel matrix.
abstract: The cell immobilization potential of a novel xylan based disulfide-crosslinked hydrogel matrix reinforced with cellulose nanocrystals was studied with continuous cultivation of Propionibacterium acidipropionici using various dilution rates. The cells were immobilized to hydrogel beads suspended freely in the fermentation broth or else packed into a column connected to a stirred tank reactor. The maximum propionic acid productivity for the combined stirred tank and column was 0.88gL(-1)h(-1) and the maximum productivity for the column was determined to be 1.39gL(-1)h(-1). The maximum propionic acid titer for the combined system was 13.9gL(-1) with a dilution rate of 0.06h(-1). Dry cell density of 99.7gL(-1) was obtained within the column packed with hydrogel beads and productivity of 1.02gL(-1)h(-1) was maintained in the column even with the high circulation rate of 3.37h(-1).
authors: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland. Electronic address: janne.wallenius@aalto.fi.
authors: Janne
authors: J
authors: Wallenius
authors: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
authors: Nikolaos
authors: N
authors: Pahimanolis
authors: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
authors: Justin
authors: J
authors: Zoppe
authors: Finnish Natural Resources Institute (Luke), Jokiniemenkuja 1, P.O. Box 18, 01301 Vantaa, Finland.
authors: Petri
authors: P
authors: Kilpeläinen
authors: Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada.
authors: Emma
authors: E
authors: Master
authors: Finnish Natural Resources Institute (Luke), Jokiniemenkuja 1, P.O. Box 18, 01301 Vantaa, Finland.
authors: Hannu
authors: H
authors: Ilvesniemi
authors: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
authors: Jukka
authors: J
authors: Seppälä
authors: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
authors: Tero
authors: T
authors: Eerikäinen
authors: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.
authors: Heikki
authors: H
authors: Ojamo
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism*; D005285:Fermentation; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D011422:Propionates / Q000032:analysis / Q000378:metabolism; D011424:Propionibacterium / Q000378:metabolism*; D014990:Xylans / Q000737:chemistry*
chemical_list: D011422:Propionates; D014990:Xylans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C029658:propionic acid
keywords: Cell immobilization; Hydrogel; P. acidipropionici; Propionic acid; Xylan