title: Overexpression of CCT8 and its significance for tumor cell proliferation, migration and invasion in glioma.
abstract: Overexpression of chaperonin containing t-complex polypeptide 1 (TCP1), or CCT, has been reported in various classes of malignancies. However, little is known about the expression of t-complex protein subunits TCP1theta (CCT8) in gliomas. In this study, the expression of CCT8 protein was detected using blotting analysis and immunohistochemistry. CCT8 was found to be overexpressed in gliomas and to correlate with the WHO grade of gliomas. To further investigate the biological function of CCT8 in gliomas, CCT8-silenced U87 glioblastoma multiforme (GBM) and U251MG cells were constructed using a small interference RNA (siRNA) sequence. The knockdown effect of CCT8 on proliferation and invasion in these cells was analyzed using the CCK8, flow cytometry cycle, scratch, transwell invasion and fluorescence assays. Compared with the controls, the glioma cells expressing CCT8-siRNA exhibited a significantly decreased proliferation and invasion capacity, as well as a dysregulated cell cytoskeleton. This study showed that high CCT8 protein expression might be related to poor outcome of glioma, and that CCT8 regulates the proliferation and invasion of glioblastomas.
authors: Department of Neurosurgery, Affiliated Hospital, Nantong University, Nantong 226001, PR China.
authors: Xiaojun
authors: X
authors: Qiu
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China.
authors: Xiaojuan
authors: X
authors: He
authors: Department of Neurosurgery, Affiliated Hospital, Nantong University, Nantong 226001, PR China.
authors: Qingfeng
authors: Q
authors: Huang
authors: Department of Neurosurgery, Affiliated Hospital, Nantong University, Nantong 226001, PR China.
authors: Xianchen
authors: X
authors: Liu
authors: Department of Neurosurgery, Affiliated Hospital, Nantong University, Nantong 226001, PR China.
authors: Guan
authors: G
authors: Sun
authors: Department of Neurosurgery, Affiliated Hospital, Nantong University, Nantong 226001, PR China.
authors: Jun
authors: J
authors: Guo
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China.
authors: Damin
authors: D
authors: Yuan
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China.
authors: Lixiang
authors: L
authors: Yang
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China.
authors: Na
authors: N
authors: Ban
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China.
authors: Shaochen
authors: S
authors: Fan
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China. Electronic address: nttaotao@ntu.edu.cn.
authors: Tao
authors: T
authors: Tao
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathology, Medical College, Nantong University, Nantong 226001, PR China. Electronic address: donglin@ntu.edu.cn.
authors: Donglin
authors: D
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000235:genetics*; D056404:Chaperonin Containing TCP-1 / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans
chemical_list: C000610437:CCT8 protein, human; D056404:Chaperonin Containing TCP-1
keywords: CCT8; Glioma; Invasion; Proliferation