title: Low expression of Gria1 and Grin1 glutamate receptors in the nucleus accumbens of Spontaneously Hypertensive Rats (SHR).
abstract: The Spontaneously Hypertensive Rat (SHR) strain is a classical animal model for the study of essential hypertension. Recently, our group suggested that this strain could be a useful animal model for schizophrenia, which is a severe mental illness with involvement of glutamatergic system. The aim of this study is to investigate glutamatergic receptors (Gria1 and Grin1) and glycine transporter (Glyt1) gene expression in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) of SHR animals. The effects in gene expression of a chronic treatment with antipsychotic drugs (risperidone, haloperidol and clozapine) were also analyzed. Animals were treated daily for 30 days, and euthanized for brain tissue collection. The expression pattern was evaluated by Real Time Reverse-Transcriptase (RT) PCR technique. In comparison to control rats, SHR animals present a lower expression of both NMDA (Grin1) and AMPA (Gria1) gene receptors in the NAcc. Antipsychotic treatments were not able to change gene expressions in any of the regions evaluated. These findings provide evidence for the role of glutamatergic changes in schizophrenia-like phenotype of the SHR strain.
authors: LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil; Department of Pharmacology, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo 669, 5th floor, CEP 04039032, Brazil.
authors: Mariana C
authors: MC
authors: Diana
authors: Genetics Division, Department of Morphology and Genetics, Universidade Federal de Sao Paulo (UNIFESP), Rua Botucatu, 740, Edifício Leitao da Cunha, 1º andar, CEP 04023-900 São Paulo, Brazil; LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil.
authors: Marcos L
authors: ML
authors: Santoro
authors: Genetics Division, Department of Morphology and Genetics, Universidade Federal de Sao Paulo (UNIFESP), Rua Botucatu, 740, Edifício Leitao da Cunha, 1º andar, CEP 04023-900 São Paulo, Brazil.
authors: Gabriela
authors: G
authors: Xavier
authors: LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil; Department of Pharmacology, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo 669, 5th floor, CEP 04039032, Brazil.
authors: Camila Mauricio
authors: CM
authors: Santos
authors: Genetics Division, Department of Morphology and Genetics, Universidade Federal de Sao Paulo (UNIFESP), Rua Botucatu, 740, Edifício Leitao da Cunha, 1º andar, CEP 04023-900 São Paulo, Brazil; LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil.
authors: Leticia N
authors: LN
authors: Spindola
authors: Genetics Division, Department of Morphology and Genetics, Universidade Federal de Sao Paulo (UNIFESP), Rua Botucatu, 740, Edifício Leitao da Cunha, 1º andar, CEP 04023-900 São Paulo, Brazil; LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil.
authors: Patrícia N
authors: PN
authors: Moretti
authors: Genetics Division, Department of Morphology and Genetics, Universidade Federal de Sao Paulo (UNIFESP), Rua Botucatu, 740, Edifício Leitao da Cunha, 1º andar, CEP 04023-900 São Paulo, Brazil; LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil.
authors: Vanessa K
authors: VK
authors: Ota
authors: LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil.
authors: Rodrigo A
authors: RA
authors: Bressan
authors: LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil; Department of Pharmacology, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo 669, 5th floor, CEP 04039032, Brazil.
authors: Vanessa C
authors: VC
authors: Abilio
authors: Genetics Division, Department of Morphology and Genetics, Universidade Federal de Sao Paulo (UNIFESP), Rua Botucatu, 740, Edifício Leitao da Cunha, 1º andar, CEP 04023-900 São Paulo, Brazil; LiNC - Interdisciplinary Laboratory of Clinical Neurosciences, Department of Psychiatry, UNIFESP, Rua Pedro de Toledo, 669, 3º floor, CEP 05039-032 São Paulo, Brazil. Electronic address: sinbelangero@gmail.com.
authors: Sintia I
authors: SI
authors: Belangero
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D003024:Clozapine / Q000494:pharmacology; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects*; D050487:Glycine Plasma Membrane Transport Proteins / Q000187:drug effects / Q000235:genetics*; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D018091:Receptors, AMPA / Q000187:drug effects / Q000235:genetics*; D017470:Receptors, Glutamate / Q000187:drug effects; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018967:Risperidone / Q000494:pharmacology; D012559:Schizophrenia / Q000503:physiopathology
chemical_list: D014150:Antipsychotic Agents; D003024:Clozapine; D050487:Glycine Plasma Membrane Transport Proteins; D006220:Haloperidol; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D018967:Risperidone; C493266:Slc6a9 protein, rat
keywords: Animal model; Glutamatergic system; SHR; Schizophrenia