title: Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation.
abstract: The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].
authors: Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors: A
authors: A
authors: Peláez-García
authors: Biochemistry and Molecular Biology I Department, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors: R
authors: R
authors: Barderas
authors: Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors: M
authors: M
authors: Mendes
authors: Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors: M
authors: M
authors: Lopez-Lucendo
authors: Department of Human Protein Sciences, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors: J C
authors: JC
authors: Sanchez
authors: IMIM-Hospital del Mar, Barcelona, Spain.
authors: A
authors: A
authors: García de Herreros
authors: Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors: J I
authors: JI
authors: Casal
keywords: Adipogenesis; Differentiation; IL-17; Mesenchymal cells; Obesity; Proteomics; Snail; Transcription factors