title: IFDOTMETER: A New Software Application for Automated Immunofluorescence Analysis.
abstract: Most laboratories interested in autophagy use different imaging software for managing and analyzing heterogeneous parameters in immunofluorescence experiments (e.g., LC3-puncta quantification and determination of the number and size of lysosomes). One solution would be software that works on a user's laptop or workstation that can access all image settings and provide quick and easy-to-use analysis of data. Thus, we have designed and implemented an application called IFDOTMETER, which can run on all major operating systems because it has been programmed using JAVA (Sun Microsystems). Briefly, IFDOTMETER software has been created to quantify a variety of biological hallmarks, including mitochondrial morphology and nuclear condensation. The program interface is intuitive and user-friendly, making it useful for users not familiar with computer handling. By setting previously defined parameters, the software can automatically analyze a large number of images without the supervision of the researcher. Once analysis is complete, the results are stored in a spreadsheet. Using software for high-throughput cell image analysis offers researchers the possibility of performing comprehensive and precise analysis of a high number of images in an automated manner, making this routine task easier.
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain.
authors: Mario
authors: M
authors: Rodríguez-Arribas
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain.
authors: Elisa
authors: E
authors: Pizarro-Estrella
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain Department of Cell Biology, University Medical Center Groningen, the Netherlands.
authors: Rubén
authors: R
authors: Gómez-Sánchez
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain.
authors: S M S
authors: SM
authors: Yakhine-Diop
authors: Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain.
authors: Antonio
authors: A
authors: Gragera-Hidalgo
authors: IEEE Engineering in Medicine and Biology Society, Cáceres, Spain Centro de Cirugía de Mínima Invasión Jesús Usón (CCMIJU), Cáceres, Spain.
authors: Alejandro
authors: A
authors: Cristo
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Equipe 11 labellisée pas la Ligue Nationale contre le Cancer, Centre de Recherche des Cordeliers, Paris, France INSERM, Paris, France Gustave Roussy Cancer Campus, Villejuif, France.
authors: Jose M
authors: JM
authors: Bravo-San Pedro
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain.
authors: Rosa A
authors: RA
authors: González-Polo
authors: Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Cáceres, Spain Departamento de Bioquímica, Biología Molecular y Genética, F. Enfermería y Terapia Ocupacional, Universidad de Extremadura, Cáceres, Spain jfuentes@unex.es rosapolo@unex.es.
authors: José M
authors: JM
authors: Fuentes
mesh_terms: D005455:Fluorescent Antibody Technique / Q000379:methods*; D057166:High-Throughput Screening Assays; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software
keywords: IFDOTMETER; autophagy; cell image analysis; immunofluorescence; software