title: (13)C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides.
abstract: The oleaginous fungus Mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid γ-linolenic acid. M. circinelloides CBS 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while M. circinelloides WJ11 can accumulate lipid up to 36%. In order to better understand the mechanisms behind the differential lipid accumulation in these two strains, tracer experiments with (13)C-glucose were performed with the growth of M. circinelloides and subsequent gas chromatography-mass spectrometric detection of (13)C-patterns in proteinogenic amino acids was carried out to identify the metabolic network topology and estimate intracellular fluxes. Our results showed that the high oleaginous strain WJ11 had higher flux of pentose phosphate pathway and malic enzyme, lower flux in tricarboxylic acid cycle, higher flux in glyoxylate cycle and ATP: citrate lyase, together, it might provide more NADPH and substrate acetyl-CoA for fatty acid synthesis.
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
authors: Lina
authors: L
authors: Zhao
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
authors: Huaiyuan
authors: H
authors: Zhang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
authors: Liping
authors: L
authors: Wang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi 214122, PR China.
authors: Haiqin
authors: H
authors: Chen
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi 214122, PR China.
authors: Yong Q
authors: YQ
authors: Chen
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi 214122, PR China.
authors: Wei
authors: W
authors: Chen
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China. Electronic address: ysong@jiangnan.edu.cn.
authors: Yuanda
authors: Y
authors: Song
mesh_terms: D002247:Carbon Isotopes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology*; D064688:Metabolic Flux Analysis; D053858:Metabolic Networks and Pathways; D009089:Mucor / Q000737:chemistry / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes
keywords: Lipid accumulation; Metabolic flux analysis; Mucor circinelloides; Pentose phosphate pathway