title: Mesoporous carbon-enriched palladium nanostructures with redox activity for enzyme-free electrochemical immunoassay of brevetoxin B.
abstract: A new signal amplification strategy based on mesoporous carbon-enriched palladium nanostructure (MSC-PdNS) was designed for enzyme-free electrochemical immunoassay of brevetoxin B (BTB) in marine toxins. The assay was carried out on a BTB-bovine serum albumin-functionalized electrode by using monoclonal mouse anti-BTB-labeling MSC-PdNS as the signal-transduction tag. A competitive-type assay protocol was successfully introduced to develop a high-efficiency enzyme-free immunoassay accompanying the doped palladium nanostructure into MSC-PdNS toward reduction of H2O2. Under the optimal conditions, the catalytic current decreased with the increment of BTB concentration in the range from 0.01 to 10 ng mL(-1) with a detection limit (LOD) of 5.0 pg mL(-1) BTB at the 3s(blank) criterion. The selectivity and precision were acceptable. In addition, the methodology was further validated for assaying spiked seafood samples, and consistent results between the electrochemical immunoassay and the referenced enzyme immunoassay were obtained. Importantly, the enzyme-free electrochemical immunoassay provides a promising approach for rapid screening of marine toxin because of its simplicity, low cost, sensitivity, specificity and without the need of sample pretreatment.
authors: Institute of Nanomedicine and Nanobiosensing, MOE & Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors: Youxiu
authors: Y
authors: Lin
authors: Institute of Nanomedicine and Nanobiosensing, MOE & Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors: Qian
authors: Q
authors: Zhou
authors: Institute of Nanomedicine and Nanobiosensing, MOE & Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors: Yuping
authors: Y
authors: Lin
authors: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China.
authors: Minghua
authors: M
authors: Lu
authors: Institute of Nanomedicine and Nanobiosensing, MOE & Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China. Electronic address: dianping.tang@fzu.edu.cn.
authors: Dianping
authors: D
authors: Tang
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D049879:Mytilidae / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D035964:Oxocins / Q000032:analysis*; D010165:Palladium / Q000737:chemistry*; D016062:Porosity; D017747:Seafood / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D000095702:Polyether Toxins
chemical_list: D055910:Antibodies, Immobilized; D002244:Carbon; D006861:Hydrogen Peroxide; D055767:Immobilized Proteins; D008387:Marine Toxins; D035964:Oxocins; D010165:Palladium; D012710:Serum Albumin, Bovine; C043466:brevetoxin B; D000095702:Polyether Toxins
keywords: Brevetoxin B; Electrochemical immunosensor; Enzyme-free immunoassay; Marine toxin; Mesoporous carbon-enriched palladium nanostructure