title: Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures.
abstract: Self-assembly is a ubiquitous approach to the design and fabrication of novel supermolecular architectures. Here we report a strategy termed 'lipid-bilayer-assisted self-assembly' that is used to assemble DNA origami nanostructures into two-dimensional lattices. DNA origami structures are electrostatically adsorbed onto a mica-supported zwitterionic lipid bilayer in the presence of divalent cations. We demonstrate that the bilayer-adsorbed origami units are mobile on the surface and self-assembled into large micrometre-sized lattices in their lateral dimensions. Using high-speed atomic force microscopy imaging, a variety of dynamic processes involved in the formation of the lattice, such as fusion, reorganization and defect filling, are successfully visualized. The surface modifiability of the assembled lattice is also demonstrated by in situ decoration with streptavidin molecules. Our approach provides a new strategy for preparing versatile scaffolds for nanofabrication and paves the way for organizing functional nanodevices in a micrometer space.
authors: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.|CREST, Japan Science and Technology Agency (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors: Yuki
authors: Y
authors: Suzuki
authors: CREST, Japan Science and Technology Agency (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.|Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Masayuki
authors: M
authors: Endo
authors: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.|CREST, Japan Science and Technology Agency (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.|Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Hiroshi
authors: H
authors: Sugiyama
mesh_terms: D000538:Aluminum Silicates; D004247:DNA; D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D055672:Static Electricity
chemical_list: D000538:Aluminum Silicates; D008051:Lipid Bilayers; D004247:DNA; C011934:mica