title: Hesperetin Modifies the Composition of Fecal Microbiota and Increases Cecal Levels of Short-Chain Fatty Acids in Rats.
abstract: There has been particular interest in the prebiotic-like effects of commonly consumed polyphenols. This study aimed to evaluate the effects of hesperidin (HD) and its aglycone hesperetin (HT), major flavonoids in citrus fruits, on the structure and activity of gut microbiota in rats. Rats ingested an assigned diet (a control diet, a 0.5% HT diet, or a 1.0% HD diet) for 3 weeks. Terminal restriction fragment length polymorphism analysis revealed that the proportion of Clostridium subcluster XIVa in the feces collected at the third week of feeding was significantly reduced by the HT diet: 19.8 ± 4.3% for the control diet versus 5.3 ± 1.5% for the HT diet (P < 0.01). There was a significant difference in the cecal pool of short-chain fatty acids (SCFA), the sum of acetic, propionic, and butyric acids, between the control diet (212 ± 71 μmol) and the HT diet (310 ± 51 μmol) (P < 0.05), whereas the HD diet exhibited no effects (245 ± 51 μmol). Interestingly, dietary HT resulted in a significant increase in the excretion of starch in the feces. HT, but not HD, might reduce starch digestion, and parts of undigested starch were utilized to produce SCFA by microbial fermentation in the large intestine.
authors: Department of Health and Nutrition, Tokyo Kasei Gakuin University , 22 Sanban-cho, Chiyoda-ku, Tokyo 102-8341, Japan.
authors: Tomonori
authors: T
authors: Unno
authors: TechnoSuruga Laboratory Company, Ltd., 330 Nagasaki, Shimizu-ku, Shizuoka 424-0065, Japan.
authors: Takayoshi
authors: T
authors: Hisada
authors: TechnoSuruga Laboratory Company, Ltd., 330 Nagasaki, Shimizu-ku, Shizuoka 424-0065, Japan.
authors: Shunsuke
authors: S
authors: Takahashi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D002432:Cecum / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D006569:Hesperidin / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005232:Fatty Acids, Volatile; D006569:Hesperidin; C013015:hesperetin
keywords: Clostridium subcluster XIVa; gut microbiota; hesperetin; hesperidin; short-chain fatty acids; starch digestion