title: Investigation of the effectiveness of disinfectants against planktonic and biofilm forms of P. aeruginosa and E. faecalis cells using a compilation of cultivation, microscopic and flow cytometric techniques.
abstract: This study evaluated the effectiveness of selected disinfectants against bacterial cells within a biofilm using flow cytometry, the conventional total viable count test and scanning electron microscopy (SEM). A flow cytometric procedure based on measurement of the cellular redox potential (CRP) was demonstrated to have potential for the rapid evaluation of activity against biofilm and planktonic forms of microbes. Quaternary ammonium compound-based disinfectant (QACB) demonstrated a higher level of anti-microbial activity than a performic acid preparation (PAP), with mean CRP values against P. aeruginosa cells of 2 and 1.33 relative fluorescence units (RFU) vs 63.33 and 61.33 RFU for 8 and 24 h cultures respectively. Flow cytometric evaluation of the anti-biofilm activity demonstrated a higher efficacy of QACB compared to PAP for P. aeruginosa cells of 1 and 0.66 RFU vs 18.33 and 22.66 RFU for 8 and 24 h cultures respectively. SEM images of treated P. aeruginosa cells demonstrated disinfectant-specific effects on cell morphology.
authors: a Department of Biotechnology and Food Microbiology , Poznań University of Life Sciences , Poznań , Poland.
authors: Wojciech
authors: W
authors: Juzwa
authors: Kamila
authors: K
authors: Myszka
authors: Wojciech
authors: W
authors: Białas
authors: Renata
authors: R
authors: Dobrucka
authors: Piotr
authors: P
authors: Konieczny
authors: Katarzyna
authors: K
authors: Czaczyk
mesh_terms: D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D004202:Disinfectants / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010933:Plankton / Q000187:drug effects / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*
chemical_list: D004202:Disinfectants; D000644:Quaternary Ammonium Compounds
keywords: biofilm; disinfectant; flow cytometric analysis; germicidal activity; technological surface