title: Characterization of triosephosphate isomerase from Mycoplasma gallisepticum.
abstract: Triosephosphate isomerase (Tpi) is a glycolytic enzyme that is essential for efficient energy production in many pathogens. However, its function in Mycoplasma gallisepticum has not been fully elucidated. In this study, the mga0357 gene of M. gallisepticum, which encodes TpiA (MGTpiA), was amplified and expressed in Escherichia coli by IPTG induction. The purified recombinant MGTpiA protein exhibited catalytic activity that was similar to TPI from rabbit muscle, reducing NAD(+) to NADH. The MGTpiA was also found to be a surface-exposed protein by western blotting and immunofluorescence assays. In addition, cytadherence inhibition assays confirmed that the cytadherence of M. gallisepticum to the DF-1 cells was significantly inhibited by the anti-MGTpiA serum. The results of the study suggested that MGTpiA plays an important role in the metabolism and closely related to the M. gallisepticum pathogenicity.
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R. China.
authors: Shijun
authors: S
authors: Bao
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Danqing
authors: D
authors: Chen
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Shengqing
authors: S
authors: Yu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Hongjun
authors: H
authors: Chen
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Lei
authors: L
authors: Tan
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Meirong
authors: M
authors: Hu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Xusheng
authors: X
authors: Qiu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China.
authors: Cuiping
authors: C
authors: Song
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. China shoveldeen@shvri.ac.cn.
authors: Chan
authors: C
authors: Ding
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics; D045686:Mycoplasma gallisepticum / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000378:metabolism; D014305:Triose-Phosphate Isomerase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D011994:Recombinant Proteins; D014305:Triose-Phosphate Isomerase
keywords: Mycoplasma gallisepticum; catalytic activity; cytoadherence; triosephosphate isomerase