title: Candidate Genes Expressed in Tolerant Common Wheat With Resistant to English Grain Aphid.
abstract: The English grain aphid, Sitobion avenae (F.) (Hemiptera: Aphididae), is a common worldwide pest of wheat (Triticum aestivum L.). The use of improved resistant cultivars by the farmers is the most effective and environmentally friendly method to control this aphid in the field. The winter wheat genotypes 98-10-35 and Amigo are resistant to S. avenae. To identify genes responsible for resistance to S. avenae in these genotypes, differential-display reverse transcription-polymerase chain reaction was used to identify the corresponding differentially expressed sequences in current study. Two backcross progenies were obtained by crossing the two resistant genotypes with the susceptible genotype 1376. Six potential expected-differential bands were sequenced. Lengths of the expressed sequence tags ranged from 128 to 532 bp. Although these expressed sequences were likely associated with S. avenae resistance, there was one expressed sequence tag located on 7DL chromosome, and its potential function may associate with the ability to maintain photosynthesis in wheat. That serves as an active way for tolerant common wheat with resistant to S. avenae. Cloning the full length of these sequences would help us thoroughly understand the mechanism of wheat resistance to S. avenae and be valuable for breeding cultivars with S. avenae resistance.
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors: Kun
authors: K
authors: Luo
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors: Gaisheng
authors: G
authors: Zhang
authors: College of Agronomy, Henan University of Science and Technology, Luoyang, Henan 471003, P. R. China.
authors: Chunping
authors: C
authors: Wang
authors: Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada.
authors: Thérèse
authors: T
authors: Ouellet
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors: Jingjing
authors: J
authors: Wu
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors: Qidi
authors: Q
authors: Zhu
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China. zhaohy@nwsuaf.edu.cn.
authors: Huiyan
authors: H
authors: Zhao
mesh_terms: D000818:Animals; D000898:Antibiosis; D001042:Aphids / Q000502:physiology*; D015870:Gene Expression; D017343:Genes, Plant; D005838:Genotype; D060434:Herbivory; D000069600:Plant Breeding; D014908:Triticum / Q000235:genetics*
keywords: DDRT-PCR; Sitobion avenae; backcross (BC); photosystem; resistance scale