title: In Vitro Analysis of Thymocyte Signaling.
abstract: From the moment a developing thymocyte expresses a TCR, it is subjected to numerous interactions with self-peptide/MHC complexes that determine its ultimate fate. These include death by neglect, negative selection (apoptosis and lineage deviation), positive selection, and lineage commitment. The identification of signals that govern these unique cell fates requires the ability to assess the activity, level of expression, subcellular location, and the molecular associations of numerous proteins within the developing T cell. Thus, this chapter describes methods designed to analyze thymocyte signaling under various types of peptide-based stimulation in vitro.
authors: Department of Molecular Microbiology and Immunology, Center for Cellular and Molecular Immunology, Columbia, MO, USA.
authors: Cody A
authors: CA
authors: Cunningham
authors: Emma
authors: E
authors: Teixeiro
authors: Mark A
authors: MA
authors: Daniels
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000276:immunology; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction; D060168:Thymocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides; D011948:Receptors, Antigen, T-Cell