title: Intrathymic Injection.
abstract: Intrathymic injection is used in several T cell-associated immunological studies to deliver cells or other substances directly into the thymus. Here, we describe the intrathymic injection procedure involving surgical incision of the mouse with or without a thoracotomy. Though this procedure can result in poor recovery, postsurgical complications, and distress to the animal, it is actually a simple procedure that can be carried out relatively easily and quickly with experience.
authors: Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
authors: Sugata
authors: S
authors: Manna
authors: Avinash
authors: A
authors: Bhandoola
mesh_terms: D000818:Animals; D007267:Injections / Q000379:methods*; D051379:Mice; D013908:Thoracotomy; D013950:Thymus Gland