title: Functionalized Polyacrylonitrile Nanofibrous Membranes for Covalent Immobilization of Glucose Oxidase.
abstract: Nanofibrous membrane (NFM) with uniform morphology and large surface area was prepared from 10% solution of polyacrylonitrile (PAN) in N,N-dimethylformamide by electrospinning technique. NFM was chemically modified for use as a support for the immobilization of glucose oxidase. Chemical modification of NFM was carried out by two different methods. In the first method, the cyano groups of PAN were modified to amino groups by a two-step process, while in the second method the carboxylic groups were generated first and then further reacted with hexamethylene diamine to create a reactive spacer arm for the immobilization of enzyme. Scanning electron microscopy studies showed that the surface morphology of NFM was not changed by chemical modification and its mechanical strength was improved. The immobilized glucose oxidase (GOx) retained 54 and 60% of its original activity up to 25 cycles with the PAN NFMs modified by the first and the second method, respectively. The GOx-immobilized NFM from the second method showed promising performance with higher enzyme immobilization, activity retention, and favorable kinetic parameters.
authors: James
authors: J
authors: Manuel
authors: Miso
authors: M
authors: Kim
authors: Rohini
authors: R
authors: Dharela
authors: Ghanshyam S
authors: GS
authors: Chauhan
authors: Deby
authors: D
authors: Fapyane
authors: Soo-Jin
authors: SJ
authors: Lee
authors: In Seop
authors: IS
authors: Chang
authors: Seo-Hee
authors: SH
authors: Kang
authors: Seon-Won
authors: SW
authors: Kim
authors: Jou-Hyeon
authors: JH
authors: Ahn
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D055119:Elastic Modulus; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013499:Surface Properties; D013718:Tensile Strength
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; C010504:polyacrylonitrile; D005949:Glucose Oxidase