title: Improvement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale.
abstract: An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.
authors: Department of Veterinary Medicine, Universidade do Contestado (UnC), Rua Roberto Ehlke 86, CEP 89460-000 Canoinhas, SC, Brazil. Electronic address: daniela@unc.br.
authors: Daniela
authors: D
authors: Pedrassani
authors: Department of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian and Veterinary Sciences (FCAV), Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900, Jaboticabal, SP, Brazil. Electronic address: nascimento.adjair@gmail.com.
authors: Adjair Antonio
authors: AA
authors: do Nascimento
authors: Department of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil. Electronic address: marcos_andre@fcav.unesp.br.
authors: Marcos Rogério
authors: MR
authors: André
authors: Department of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil. Electronic address: zacarias@fcav.unesp.br.
authors: Rosangela Zacarias
authors: RZ
authors: Machado
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D017159:Dioctophymatoidea / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D017189:Enoplida Infections / Q000097:blood / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D008297:Male; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth
keywords: Dioctophyma; Dogs; ELISA test; Serological diagnosis