title: FUNGAL BIOGEOGRAPHY. Response to Comment on "Global diversity and geography of soil fungi".
abstract: Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)-free shotgun metagenomics revealed that barcode and primer choice and PCR bias drive the diversity and composition of microorganisms in general, but the Archaeorhizomycetes were little affected in the global study. We urge that careful choice of DNA markers and primers is essential for ecological studies using high-throughput sequencing for identification.
authors: Natural History Museum, University of Tartu, Tartu, Estonia. leho.tedersoo@ut.ee.
authors: Leho
authors: L
authors: Tedersoo
authors: Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia. Department of Organismal Biology, Uppsala University, Uppsala, Sweden.
authors: Mohammad
authors: M
authors: Bahram
authors: Natural History Museum, University of Tartu, Tartu, Estonia.
authors: Sergei
authors: S
authors: Põlme
authors: Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia.
authors: Sten
authors: S
authors: Anslan
authors: Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia.
authors: Taavi
authors: T
authors: Riit
authors: Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia.
authors: Urmas
authors: U
authors: Kõljalg
authors: Department of Biological and Environmental Sciences, University of Gothenburg, Göteborg, Sweden.
authors: R Henrik
authors: RH
authors: Nilsson
authors: European Molecular Biology Laboratory, Heidelberg, Germany.
authors: Falk
authors: F
authors: Hildebrand
authors: Natural History Museum, University of Tartu, Tartu, Estonia.
authors: Kessy
authors: K
authors: Abarenkov
mesh_terms: D005658:Fungi / Q000145:classification* / Q000502:physiology*; D012987:Soil; D012988:Soil Microbiology
chemical_list: D012987:Soil