title: Sphingobacterium rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis.
abstract: A novel Gram-negative, strictly aerobic, short rod-shaped, non-motile bacterium, designated YIM 101302(T), was isolated from the faeces of Rhinoceros unicornis dwelling in the Yunnan Wild Animal Park, Yunnan province, South-West China. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain YIM 101302(T) to the genus Sphingobacterium. The newly isolated bacterium was found to be closely related to Sphingobacterium composti T5-12(T) (97.1% 16S rRNA sequence identity) and Sphingobacterium alimentarium WCC 4521(T) (95.6% 16S rRNA sequence identity) forming a distinct clade with these two species. Polar lipids of strain YIM 101302(T) were identified as phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified aminophospholipid, and three unidentified polar lipids; the predominant menaquinone as MK-7 and the major fatty as iso-C15:0. The genomic DNA G+C content was determined to be 38.9 mol%. The DNA-DNA hybridization values between strain YIM 101302(T) and S. composti T5-12(T), was 53.6 ± 5.8%. These results indicates that strain YIM 101302(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium rhinocerotis sp. nov. is proposed. The type strain is YIM 101302(T) (=CCTCC AB 2013218(T) = KCTC 42533(T)).
authors: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
authors: Gui-Ding
authors: GD
authors: Li
authors: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang, 110819, People's Republic of China.
authors: Xiu
authors: X
authors: Chen
authors: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.|Life Sciences Lab Center, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
authors: Qin-Yuan
authors: QY
authors: Li
authors: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
authors: Fang-Ji
authors: FJ
authors: Xu
authors: Yunnan Wild Animal Park, Kunming, 650218, People's Republic of China.
authors: Shu-Mei
authors: SM
authors: Qiu
authors: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China. jiangyikm@hotmail.com.
authors: Yi
authors: Y
authors: Jiang
authors: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
authors: Cheng-Lin
authors: CL
authors: Jiang
mesh_terms: D000818:Animals; D005243:Feces / Q000382:microbiology*; D050356:Lipid Metabolism; D010527:Perissodactyla / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Faeces; Rhinoceros unicornis; Sphingobacterium rhinocerotis sp. nov.