title: Bacillus gossypii sp. nov., isolated from the stem of Gossypium hirsutum.
abstract: A Gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of Gossypium hirsutum, was studied to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain JM-267T was grouped in the genus Bacillus, related most closely to the type strains of Bacillus simplex and Bacillus huizhouensis (both 97.8%), Bacillus muralis (97.7%), Bacillus butanolivorans and Bacillus psychrosaccharolyticus (both 97.3%). 16S rRNA gene sequence similarity to the sequences of the type strains of other Bacillus species was < 97.0%. The fatty acid profile supported the grouping of the strain to the genus Bacillus. As major fatty acids, anteiso-C15:0, iso-C15:0, iso-C14:0 and iso-C16:0 were detected. The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major quinone was menaquinone 7 (MK-7). DNA-DNA hybridizations with B. simplex DSM 1321T, B. huizhouensis GSS03T, B. muralis LMG 20238T, B. butanolivorans LMG 23974T and B. psychrosaccharolyticus DSM 6T resulted in values clearly below 70%. In addition, physiological and biochemical test results allowed the clear phenotypic differentiation of strain JM-267T from the most closely related species. Hence, strain JM-267T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus gossypii sp. nov. is proposed. The type strain is JM-267T ( = DSM 100034T = LMG 28742T).
authors: Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
authors: Peter
authors: P
authors: Kämpfer
authors: Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors: Hans-Jürgen
authors: HJ
authors: Busse
authors: Department of Entomology and Plant Pathology, Auburn University, AL 36849, USA.
authors: John A
authors: JA
authors: McInroy
authors: Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
authors: Stefanie P
authors: SP
authors: Glaeser
mesh_terms: D000407:Alabama; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D003368:Gossypium / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology*; D011073:Polyamines / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7