title: SIX1 overexpression in diffuse-type and grade III gastric tumors: Features that are associated with poor prognosis.
abstract: BACKGROUND
  Gastric cancer is the second most common cancer worldwide. In Iran, the incidence of gastric cancer is well above the world average, and is the first common cancer in Iranian men and the third one in women. Located at chromosome 14q23, SIX1 is a homolog of the Drosophila 'sine oculis' (so) gene and is highly conserved in numerous species. In addition to the role of SIX1 in the development, its expression is frequently dysregulated in multiple cancers. This study aimed to evaluate the clinicopathological features of the expression of SIX1 gene in gastric adenocarcinoma.
  
  
  METHODS
  Thirty pairs of gastric tissue samples from patients with gastric adenocarcinoma were evaluated for SIX1 gene expression using quantitative real-time polymerase chain reaction. A paired t-test or one-way ANOVA with post hoc multiple comparisons were used to analyze the differences between groups. Statistical significance was defined as P ≤ 0.05.
  
  
  RESULTS
  SIX1 expression was decreased in tumoral samples. However, its expression increased significantly in diffuse-type gastric cancer. Furthermore, there was a trend toward statistical significance in increasing SIX1 gene expression with higher grades. Of note, the difference was significant between grades I and III.
  
  
  CONCLUSIONS
  The results suggest that SIX1 gene expression might be used in the future as a potential biomarker to predict the outcome of the disease as diffuse-type and grade III of gastric tumors are associated with poor prognosis.
authors: Department of Genetics, Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
authors: Modjtaba
authors: M
authors: Emadi-Baygi
authors: Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran ; Pediatric Inherited Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Child Growth and Development Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Parvaneh
authors: P
authors: Nikpour
authors: Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Elaheh
authors: E
authors: Emadi-Andani
keywords: Diffuse-type gastric cancer; gene expression; poor prognosis SIX1; tumor grades