title: A novel type of matrix for surface-assisted laser desorption-ionization mass spectrometric detection of biomolecules using metal-organic frameworks.
abstract: A 3D metal-organic framework (MOF) nanomaterial as matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and tandem mass spectrometry (MS/MS) was developed for the analysis of complex biomolecules. Unlike other nanoparticle matrices, this MOF nanomaterial does not need chemical modification prior to use. An exceptional signal reproducibility as well as very low background interferences in analyzing mono-/di-saccharides, peptides and complex starch digests demonstrate its high potential for biomolecule assays, especially for small molecules.
authors: Department of Chemistry, Chung Yuan Christian University, Chung Li District, Taoyuan City, 320, Taiwan.
authors: Chien-Ping
authors: CP
authors: Fu
authors: Department of Chemistry, Chung Yuan Christian University, Chung Li District, Taoyuan City, 320, Taiwan.
authors: Stephen
authors: S
authors: Lirio
authors: Department of Chemistry, Chung Yuan Christian University, Chung Li District, Taoyuan City, 320, Taiwan.
authors: Wan-Ling
authors: WL
authors: Liu
authors: Department of Chemistry, Chung Yuan Christian University, Chung Li District, Taoyuan City, 320, Taiwan. Electronic address: chiaher@cycu.edu.tw.
authors: Chia-Her
authors: CH
authors: Lin
authors: Department of Chemistry, Chung Yuan Christian University, Chung Li District, Taoyuan City, 320, Taiwan. Electronic address: hyhuang@cycu.edu.tw.
authors: Hsi-Ya
authors: HY
authors: Huang
mesh_terms: D004186:Disaccharidases / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013213:Starch / Q000032:analysis*; D013499:Surface Properties
chemical_list: D009005:Monosaccharides; D009942:Organometallic Compounds; D010455:Peptides; D011506:Proteins; D013213:Starch; D004186:Disaccharidases
keywords: Biomolecules; Carbohydrates; Metal-organic frameworks; Peptides; Surface-assisted laser desorption–ionization mass spectrometry