title: LOX-1 gene variants and maternal levels of plasma oxidized LDL and malondialdehyde in patients with gestational diabetes mellitus.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationships between the maternal levels of oxidized LDL (ox-LDL), lipid peroxidation marker malondialdehyde (MDA) and LOX-1 3'UTR188C/T and K167N single nucleotide polymorphisms in pregnant Turkish women with gestational diabetes mellitus (GDM).
  
  
  METHODS
  116 pregnant women with GDM and 120 healthy pregnant women from the same geographic region were included in the study. Polymerase chain reaction-based restriction analysis was used to identify 3'UTR188C/T and K167N polymorphisms of the LOX-1 gene. Plasma ox-LDL and MDA levels were determined by enzyme-linked immunosorbent assay and spectrophotometric method in all study subjects, respectively.
  
  
  RESULTS
  Our results indicated that the distribution of the LOX-1 3'UTR188C/T and K167N genotypes and alleles did not differ significantly among subjects with or without GDM (p > 0.05). TT and NN genotype carriers are associated with some glucose metabolism parameters (p < 0.05). There were no significant differences among plasma ox-LDL and MDA levels with regard to LOX-1 3'UTR188C/T and K167N polymorphisms in GDM group and control subjects (p > 0.05). According to the combined genotype analysis of LOX-1 3'UTR 188 TT and K167N NN polymorphisms, plasma MDA and ox-LDL levels were significantly different between women with GDM and healthy subjects either with or without combined TT/NN genotype carriers (p < 0.001).
  
  
  CONCLUSIONS
  According to our results, ox-LDL and MDA levels were increased in GDM pregnant women and healthy pregnant women either with or without combined TT/NN genotype carriers, for our Turkish sample, these genotype carriers appear to be related with increased oxidative stress in patients with GDM.
authors: Department of Biophysics, Medical Faculty, Sakarya University, 54290, Sakarya, Turkey. birsenay2001@yahoo.com.
authors: Birsen
authors: B
authors: Aydemir
authors: Department of Medical Biology, Cerrahpaşa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey.
authors: Onur
authors: O
authors: Baykara
authors: Department of Biochemistry, Medical Faculty, Sakarya University, 54290, Sakarya, Turkey.
authors: Fatma Behice Serinkan
authors: FB
authors: Cinemre
authors: Department of Internal Medicine, Medical Faculty, Sakarya University, 54290, Sakarya, Turkey.
authors: Hakan
authors: H
authors: Cinemre
authors: Departments of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey.
authors: Abdullah
authors: A
authors: Tuten
authors: Department of Biophysics, Medical Faculty, Namık Kemal University, 59100, Tekirdag, Turkey.
authors: Ali Riza
authors: AR
authors: Kiziler
authors: Departments of Obstetrics and Gynecology, Medical Faculty, Sakarya University, 54290, Sakarya, Turkey.
authors: Nermin
authors: N
authors: Akdemir
authors: Departments of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey.
authors: Mahmut
authors: M
authors: Oncul
authors: Departments of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey.
authors: Baris
authors: B
authors: Kaya
authors: Department of Biochemistry, Yildiz Technical University, 34220, Istanbul, Turkey.
authors: Volkan
authors: V
authors: Sozer
authors: Department of Biostatistic, Medical Faculty, Sakarya University, 54290, Sakarya, Turkey.
authors: Unal
authors: U
authors: Erkorkmaz
authors: Department of Biochemistry, Cerrahpaşa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey.
authors: Hafize
authors: H
authors: Uzun
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood*; D062145:Maternal Serum Screening Tests; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D051127:Scavenger Receptors, Class E / Q000097:blood / Q000235:genetics*; D013053:Spectrophotometry; D014421:Turkey
chemical_list: D020413:3' Untranslated Regions; D008077:Lipoproteins, LDL; C495137:OLR1 protein, human; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde
keywords: Gestational diabetes mellitus; Lectin-like oxidized LDL receptor-1 gene polymorphism; Malondialdehyde; Oxidized LDL