title: Redox activity of melanin from the ink sac of Sepia officinalis by means of colorimetric oxidative assay.
abstract: The redox properties of natural extract from cuttlefish ink sac (Sepia officinalis) and synthetic melanin used as a biomimetic in melanin structural investigation were determined by comparison of this phenol-based heterogeneous pigment with gallic acid used as a standard in Folin-Ciocalteu colorimetric assay widely employed for characterisation of oxidative properties of biomaterials. Reactivity of sepia melanin reported here is much higher than previously indicated and this protocol should allow the redox characterisation of all melanins irrespective of their origin and composition.
authors: a 3B's Research Group - Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , University of Minho , Guimarães , Portugal.|b PT Government Associate Laboratory , ICVS/3B's , Guimarães , Portugal.|c Faculty of Pharmaceutical Sciences , Khon Kaen University , Khon Kaen , Thailand.
authors: Pathomthat
authors: P
authors: Srisuk
authors: a 3B's Research Group - Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , University of Minho , Guimarães , Portugal.|b PT Government Associate Laboratory , ICVS/3B's , Guimarães , Portugal.
authors: Vitor M
authors: VM
authors: Correlo
authors: a 3B's Research Group - Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , University of Minho , Guimarães , Portugal.|b PT Government Associate Laboratory , ICVS/3B's , Guimarães , Portugal.
authors: Isabel B
authors: IB
authors: Leonor
authors: a 3B's Research Group - Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , University of Minho , Guimarães , Portugal.|b PT Government Associate Laboratory , ICVS/3B's , Guimarães , Portugal.
authors: Pasquale
authors: P
authors: Palladino
authors: a 3B's Research Group - Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , University of Minho , Guimarães , Portugal.|b PT Government Associate Laboratory , ICVS/3B's , Guimarães , Portugal.
authors: Rui L
authors: RL
authors: Reis
mesh_terms: D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D003124:Colorimetry; D008543:Melanins / Q000378:metabolism*; D010084:Oxidation-Reduction; D049833:Sepia / Q000737:chemistry*
chemical_list: D008543:Melanins
keywords: Folin–Ciocalteu phenol reagent; Sepia officinalis; cuttlefish ink sac; gallic acid; redox activity; sepia melanin; synthetic melanin