title: Rhodococcus lactis sp. nov., an actinobacterium isolated from sludge of a dairy waste treatment plant.
abstract: A Gram-stain-positive, non-motile and aerobic bacterium, designated strain DW151BT, was isolated from a sludge sample of a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis of strain DW151BT placed it within the genus Rhodococcus. It displayed significant similarity with recognized species of the genus: Rhodococcus pyridinivorans PDB9T (98.8 %), Rhodococcus gordoniae W 4937T (98.6 %), Rhodococcus rhodochrous DSM 43241T (98.5 %) and Rhodococcus artemisiae YIM 65754T (97.5 %). However, strain DW151BT differed from phylogenetically closely related species in various phenotypic properties. The cellular polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) as major lipids, MK-8(H2) was the major menaquinone and meso-diaminopimelic acid was the cell-wall peptidoglycan. The fatty acid profile consisted of C16 : 0, C18 : 1cis9 and C16 : 1cis9 as main components. The presence of C16 : 0 and diphosphatidylglycerol as major fatty acid and polar lipid, respectively, was in accordance with chemotaxonomic markers of the genus Rhodococcus. The DNA G+C content of strain DW151BT was 69.9 mol%, a value within the limits reported for the members of this genus. Furthermore, strain DW151BT showed low similarity at the whole genome level in DNA-DNA hybridization experiments with phylogenetically closely related strains. Considering the low similarity at the genome level and differences in phenotypic properties, strain DW151BT is considered to represent a novel species of the genus Rhodococcus, for which the name Rhodococcus lactis sp. nov. is proposed. The type strain is DW151BT ( = MTCC 12279T = DSM 45625T).
authors: Microbial Type Culture Collection and Gene bank MTCC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors: Pradip Kumar
authors: PK
authors: Singh
authors: Microbial Type Culture Collection and Gene bank MTCC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors: Annu
authors: A
authors: Kumari
authors: Microbial Type Culture Collection and Gene bank MTCC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors: Niharika
authors: N
authors: Chawla
authors: Microbial Type Culture Collection and Gene bank MTCC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors: Anil Kumar
authors: AK
authors: Pinnaka
authors: Microbial Type Culture Collection and Gene bank MTCC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors: Suresh
authors: S
authors: Korpole
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003612:Dairying; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid