title: Oral Administration of TAT-PTD-Diapause Hormone Fusion Protein Interferes With Helicoverpa armigera (Lepidoptera: Noctuidae) Development.
abstract: Diapause hormone (DH), which can terminate diapause in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae), has shown promise as a pest control method. However, the main challenge in using DH as an insecticide lies in achieving effective oral delivery, since the peptide may be degraded by digestive enzymes in the gut. To improve the efficacy of oral DH application, the Clostera anastomosis (L.) (Lepidoptera: Notodontidae) diapause hormone (caDH) was fused to the Protein Transduction Domain (PTD) of the human immunodeficiency virus-1 transactivator of transcription (TAT). Cellular transduction of TAT-caDH was verified with the use of a green fluorescent protein fusion, and its ability to terminate diapause was verified by injection into diapausing H. armigera pupae. Orally administered TAT-caDH resulted in larval growth inhibition. In TAT-caDH-treated insects, larval duration was delayed and the pupation rates were decreased at both development promoting conditions [27 °C, a photoperiod of 14:10(L:D) h] and diapause inducing conditions [20 °C, a photoperiod of 10:14(L:D) h]. No significant difference in diapause rate was observed between the TAT-caDH-treated and caDH-treated or control pupae maintained at diapause inducing conditions. Our results show that treatment with a recombinant TAT-caDH protein can affect larval development in H. armigera, and it suggest that TAT-DH treatment may be useful for controlling pests. This study is the first record of oral DH application in insect.
authors: College of Forestry, Henan University of Science and Technology, Luoyang 471003, People's Republic of China.
authors: Zhou
authors: Z
authors: Zhou
authors: College of Forestry, Henan University of Science and Technology, Luoyang 471003, People's Republic of China.
authors: Yongli
authors: Y
authors: Li
authors: College of Forestry, Henan University of Science and Technology, Luoyang 471003, People's Republic of China.
authors: Chunyan
authors: C
authors: Yuan
authors: Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Haidian District, Beijing 100091, People's Republic of China.
authors: Yongan
authors: Y
authors: Zhang
authors: Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Haidian District, Beijing 100091, People's Republic of China qulj2001@caf.ac.cn.
authors: Liangjian
authors: L
authors: Qu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000502:physiology; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D011679:Pupa / Q000254:growth & development / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides; D011993:Recombinant Fusion Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; C075027:diapause hormone
keywords: Helicoverpa armigera; diapause hormone; fusion protein; growth inhibition; transactivator of transcription