title: Inhibition of 4NQO-Induced Oral Carcinogenesis by Dietary Oyster Shell Calcium.
abstract: Oyster has gained much attention recently for its anticancer activity but it is unclear whether calcium, the major antitumor ingredient in oyster shell, is responsible for the anticarcinogenic role of the oyster. To address this issue, C57BL/6 mice were fed with the carcinogen 4-nitroquinoline-1-oxide (4NQO, 50 µg/mL) and normal diet or a diet containing oyster powder, oyster calcium, or calcium depleted oyster powder. The tongue tissue specimens isolated from these mice were histologically evaluated for hyperplasia, dysplasia, and papillary lesions, and then analyzed for proliferation and differentiation markers by immunohistochemistry. The results showed that mice on the diet containing oyster calcium significantly reduced rates of tumors in the tongue and proliferation and enhanced differentiation in the oral epithelium compared with the diet containing calcium depleted oyster powder. These results suggest that calcium in oyster plays a critical role in suppressing formation of oral squamous cell carcinoma and proliferation and promoting differentiation of the oral epithelium.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors: Ying
authors: Y
authors: Chen
authors: Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors: Yi
authors: Y
authors: Jiang
authors: Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors: Liyan
authors: L
authors: Liao
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors: Xiaoxin
authors: X
authors: Zhu
authors: Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors: Shengan
authors: S
authors: Tang
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors: Qing
authors: Q
authors: Yang
authors: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China.
authors: Lihua
authors: L
authors: Sun
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors: Yujie
authors: Y
authors: Li
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors: Shuangrong
authors: S
authors: Gao
authors: Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China zhongjian.xie@outlook.com.
authors: Zhongjian
authors: Z
authors: Xie
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000009:adverse effects*; D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D063646:Carcinogenesis / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000188:drug therapy; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000188:drug therapy*; D010125:Ostreidae / Q000737:chemistry*; D014059:Tongue / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D002273:Carcinogens; D015112:4-Nitroquinoline-1-oxide; D002118:Calcium
keywords: calcium; differentiation; oral carcinogenesis; oyster; proliferation