title: Value of PAX1 Methylation Analysis by MS-HRM in the Triage of Atypical Squamous Cells of Undetermined Significance.
abstract: BACKGROUND
  Detection of cervical high grade lesions in patients with atypical squamous cells of undetermined significance (ASCUS) is still a challenge. Our study tested the efficacy of the paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in ASCUS and compared performance with the hybrid capture 2 (HC2) human papillomavirus (HPV) test.
  
  
  METHODS
  A total of 463 consecutive ASCUS women from primary screening were selected. Their cervical scrapings were collected and assessed by PAX1 methylation analysis (MS-HRM) and high-risk HPV-DNA test (HC2). All patients with ASCUS were admitted to colposcopy and cervical biopsies. The Chi- square test was used to test the differences of PAX1 methylation or HPV infection between groups.
  
  
  RESULTS
  The specificity, sensitivity, and accuracy for detecting CIN2 + lesions were: 95.6%, 82.4%, and 94.6%, respectively, for the PAX1 MS-HRM test; and 59.7%, 64.7%, and 60.0% for the HC2 HPV test.
  
  
  CONCLUSIONS
  The PAX1 methylation analysis by MS-HRM demonstrated a better performance than the high-risk HPV-DNA test for the detection of high grade lesions (CIN2 +) in ASCUS cases. This approach could screen out the majority of low grade cases of ASCUS, and thus reduce the referral rate to colposcopy.
authors: Department of Clinical Laboratory, Weifang City People's Hospital, Weifang, China E-mail : wzm196@163.com.
authors: Shi-Rong
authors: SR
authors: Li
authors: Zhen-Ming
authors: ZM
authors: Wang
authors: Yu-Hui
authors: YH
authors: Wang
authors: Xi-Bo
authors: XB
authors: Wang
authors: Jian-Qiang
authors: JQ
authors: Zhao
authors: Hai-Bin
authors: HB
authors: Xue
authors: Fu-Guo
authors: FG
authors: Jiang
mesh_terms: D000328:Adult; D065309:Atypical Squamous Cells of the Cervix / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D019175:DNA Methylation; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D051761:Paired Box Transcription Factors / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; D051761:Paired Box Transcription Factors; C070835:PAX1 transcription factor