title: Mechanistic insights into the interactions of magnetic nanoparticles with bovine serum albumin in presence of surfactants.
abstract: This work reports the physicochemical parameters and the nature of association between magnetic nanoparticles and bovine serum albumin (BSA) in presence of cationic and anionic surfactants. Magnetic iron oxide nanoparticles (MNPs) are first synthesized using chemical co-precipitation method and subsequently characterized by FTIR, XRD, DLS, TEM and Zeta potential. The bare nanoparticles are then coated with BSA and their interactions studied using fluorescence spectroscopy, dynamic light scattering and circular dichroism techniques. The spectroscopic investigation sheds light into various aspects of binding and size variation during the molecular association of BSA with the MNPs in absence and presence of cationic and anionic surfactants. Isothermal titration calorimetry was used to probe the thermodynamic parameters of the systems. MNPs-BSA system was found to be more stable in presence of cationic surfactant. This study provides valuable mechanistic insights into the interactions taking place at the interface of the nanoparticles which further helps in designing a stable colloidal MNPs systems.
authors: Department of Chemical Sciences, School of Science, NMIMS University, Vile Parle (West), Mumbai 400056, India.
authors: Delina
authors: D
authors: Joseph
authors: Department of Chemistry, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400098, India.
authors: Shilpee
authors: S
authors: Sachar
authors: Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors: Nand
authors: N
authors: Kishore
authors: Department of Chemical Sciences, School of Science, NMIMS University, Vile Parle (West), Mumbai 400056, India. Electronic address: sudeshna.chandra@nmims.edu.
authors: Sudeshna
authors: S
authors: Chandra
mesh_terms: D003102:Colloids / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D003102:Colloids; D058185:Magnetite Nanoparticles; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; Interactions; Magnetic nanoparticles; Thermodynamics; surfactants