title: Optimized Production of Xylitol from Xylose Using a Hyper-Acidophilic Candida tropicalis.
abstract: The yeast Candida tropicalis DSM 7524 produces xylitol, a natural, low-calorie sweetener, by fermentation of xylose. In order to increase xylitol production rate during the submerged fermentation process, some parameters-substrate (xylose) concentration, pH, aeration rate, temperature and fermentation strategy-have been optimized. The maximum xylitol yield reached at 60-80 g/L initial xylose concentration, pH 5.5 at 37 °C was 83.66% (w/w) on consumed xylose in microaerophilic conditions (kLa = 2·h(-1)). Scaling up on 3 L fermenter, with a fed-batch strategy, the best xylitol yield was 86.84% (w/w), against a 90% of theoretical yield. The hyper-acidophilic behaviour of C. tropicalis makes this strain particularly promising for industrial application, due to the possibility to work in non-sterile conditions.
authors: Department of Life Sciences and Biotechnology, University of Ferrara, Via L. Borsari 46, Ferrara 44121, Italy. tme@unife.it.
authors: Elena
authors: E
authors: Tamburini
authors: Department of Life Sciences and Biotechnology, University of Ferrara, Via L. Borsari 46, Ferrara 44121, Italy. stefania.costa@unife.it.
authors: Stefania
authors: S
authors: Costa
authors: Department of Life Sciences and Biotechnology, University of Ferrara, Via L. Borsari 46, Ferrara 44121, Italy. mhm@unife.it.
authors: Maria Gabriella
authors: MG
authors: Marchetti
authors: Department of Life Sciences and Biotechnology, University of Ferrara, Via L. Borsari 46, Ferrara 44121, Italy. pdp@unife.it.
authors: Paola
authors: P
authors: Pedrini
mesh_terms: D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D041022:Candida tropicalis / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007101:Immersion; D013696:Temperature; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism*
chemical_list: D014994:Xylose; D014993:Xylitol
keywords: Candida tropicalis; fed batch fermentation; hyper-acidophylic; xylitol; xylose