title: Protective Effects of Bacillus subtilis ANSB060 on Serum Biochemistry, Histopathological Changes and Antioxidant Enzyme Activities of Broilers Fed Moldy Peanut Meal Naturally Contaminated with Aflatoxins.
abstract: The aim of this study was to investigate the toxic effects of aflatoxins and evaluate the effectiveness of Bacillus subtilis ANSB060 in detoxifying aflatoxicosis in broilers. A total of 360 one-week-old male broilers (Ross 308) were assigned to six dietary treatments for five weeks. The treatment diets were: C0 (basal diet); C1.0 (C0 + 1.0 g B. subtilis ANSB060/kg diet); M0 (basal diet formulated with moldy peanut meal); M0.5, M1.0 and M2.0 (M0 + 0.5, 1.0 and 2.0 g B. subtilis ANSB060/kg diet, respectively). The contents of aflatoxin B1, B2, G1 and G2 in the diets formulated with moldy peanut meal were 70.7 ± 1.3, 11.0 ± 1.5, 6.5 ± 0.8 and 2.0 ± 0.3 µg/kg, respectively. The results showed that aflatoxins increased (p < 0.05) serum aspartate transaminase activity, decreased (p < 0.05) serum glutathione peroxidase activity, and enhanced (p < 0.05) malondialdehyde contents in both the serum and liver. Aflatoxins also caused gross and histological changes in liver tissues, such as bile duct epithelium hyperplasia, vacuolar degeneration and lymphocyte infiltration. The supplementation of ANSB060 reduced aflatoxin levels in the duodenum and counteracted the negative effects of aflatoxins, leading to the conclusion that ANSB060 has a protective effect against aflatoxicosis and this protection is dose-related.
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. fanyucau@163.com.
authors: Yu
authors: Y
authors: Fan
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. lihongzhao100@126.com.
authors: Lihong
authors: L
authors: Zhao
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jicheng@cau.edu.cn.
authors: Cheng
authors: C
authors: Ji
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. li_xy8707@163.com.
authors: Xiaoying
authors: X
authors: Li
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jiaru.bjdk071@163.com.
authors: Ru
authors: R
authors: Jia
authors: Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA. xilin@ncsu.edu.
authors: Lin
authors: L
authors: Xi
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jyzhang@cau.edu.cn.
authors: Jianyun
authors: J
authors: Zhang
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. maqiugang@cau.edu.cn.
authors: Qiugang
authors: Q
authors: Ma
mesh_terms: D000348:Aflatoxins / Q000032:analysis / Q000633:toxicity*; D000821:Animal Feed / Q000009:adverse effects* / Q000032:analysis; D000818:Animals; D010367:Arachis / Q000737:chemistry / Q000382:microbiology; D001219:Aspartate Aminotransferases / Q000097:blood; D001412:Bacillus subtilis; D002645:Chickens; D019587:Dietary Supplements; D004386:Duodenum / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D005979:Glutathione Peroxidase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D000348:Aflatoxins; D020011:Protective Agents; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases
keywords: Bacillus subtilis; aflatoxin biodegradation preparation; aflatoxins; antioxidant; broiler; liver