title: Cytotoxicity of pregnane glycosides of Cynanchum otophyllum.
abstract: Fourteen new pregnane glycosides, including nine caudatin glycosides (1-9), three qinyangshengenin glycosides (10-12), one kidjoranin glycosides (13) and one gagaminin glycosides (14), along with twelve known analogs (15-26) were isolated from roots of Cynanchum otophyllum Schneid. Their structures were deduced by detailed analysis of 1D and 2D NMR spectra, as well as HRESIMS. In this study, all pregnane glycosides obtained (1-26) were evaluated for their cytotoxic activities using three cancer cell lines (HepG2, Hela, U251). As results, except 6 and 10, other twenty-four pregnane glycosides showed cytotoxicities at different degrees against three cell lines.
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors: Mi
authors: M
authors: Zhang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors: Xiang
authors: X
authors: Li
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors: Cheng
authors: C
authors: Xiang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors: Yi
authors: Y
authors: Qin
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors: Jing
authors: J
authors: He
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. Electronic address: baocaili@hotmail.com.
authors: Bao-Cai
authors: BC
authors: Li
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau 999078, China. Electronic address: pli1978@hotmail.com.
authors: Peng
authors: P
authors: Li
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031105:Cynanchum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D011278:Pregnanes; C574437:pregnane glycoside
keywords: Cynanchum otophyllum; Cytotoxicity; Pregnane glycosides