title: Wide Dispersion and Diversity of Clonally Related Inhibitory Interneurons.
abstract: The mammalian neocortex is composed of two major neuronal cell types with distinct origins: excitatory pyramidal neurons and inhibitory interneurons, generated in dorsal and ventral progenitor zones of the embryonic telencephalon, respectively. Thus, inhibitory neurons migrate relatively long distances to reach their destination in the developing forebrain. The role of lineage in the organization and circuitry of interneurons is still not well understood. Utilizing a combination of genetics, retroviral fate mapping, and lineage-specific retroviral barcode labeling, we find that clonally related interneurons can be widely dispersed while unrelated interneurons can be closely clustered. These data suggest that migratory mechanisms related to the clustering of interneurons occur largely independent of their clonal origin.
authors: Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: corey_harwell@hms.harvard.edu.
authors: Corey C
authors: CC
authors: Harwell
authors: Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, San Francisco, CA 94143, USA.
authors: Luis C
authors: LC
authors: Fuentealba
authors: Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
authors: Adrian
authors: A
authors: Gonzalez-Cerrillo
authors: Gladstone Institute for Neurological Disease, San Francisco, CA 94158, USA.
authors: Phillip R L
authors: PR
authors: Parker
authors: Department of Neurology, University of California, San Francisco, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, San Francisco, CA 94143, USA.
authors: Caitlyn C
authors: CC
authors: Gertz
authors: Department of Biostatistics, Harvard School of Public Health, Boston, MA 02115, USA.
authors: Emanuele
authors: E
authors: Mazzola
authors: Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
authors: Miguel Turrero
authors: MT
authors: Garcia
authors: Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, San Francisco, CA 94143, USA.
authors: Arturo
authors: A
authors: Alvarez-Buylla
authors: Departments of Genetics and Ophthalmology and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
authors: Constance L
authors: CL
authors: Cepko
authors: Department of Neurology, University of California, San Francisco, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, San Francisco, CA 94143, USA.
authors: Arnold R
authors: AR
authors: Kriegstein
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology*; D061346:Laser Capture Microdissection; D051379:Mice; D009415:Nerve Net / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009433:Neural Inhibition; D058953:Neural Stem Cells / Q000145:classification / Q000502:physiology*; D009924:Organ Culture Techniques; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology; D014161:Transduction, Genetic
chemical_list: D009419:Nerve Tissue Proteins; D049452:Green Fluorescent Proteins