title: Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells.
abstract: The aim of the present study was to investigate the periodontitis-associated changes in the number, proliferation and differentiation potential of human periodontal ligament stem cells (PDLSCs). Cultures of human periodontal ligament cells (PDLCs) were established from healthy donors and donors with periodontitis. The numbers of stem cell were characterized using flow cytometry. PDLSCs were isolated from the PDLCs by immunomagnetic bead selection. Colony‑forming abilities, osteogenic and adipogenic potential, gene expression of cementoblast phenotype, alkaline phosphatase activity and in vivo differentiation capacities were then evaluated. Periodontitis caused an increase in the proliferation of PDLSCs and a decrease in the commitment to the osteoblast lineage. This is reflected by changes in the expression of osteoblast markers. When transplanted into immunocompromised mice, PDLSCs from the healthy donors exhibited the capacity to produce cementum PDL‑like structures, whereas, the inflammatory PDLSCs transplants predominantly formed connective tissues. In conclusion, the data from the present study suggest that periodontitis affects the proliferation and differentiation potential of human PDLSCs in vitro and in vivo.
authors: Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin 300041, P.R. China.
authors: Wei
authors: W
authors: Zheng
authors: Department of Stomatology, The Third Central Hospital, Tianjin 300041, P.R. China.
authors: Shi
authors: S
authors: Wang
authors: Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin 300041, P.R. China.
authors: Jianguo
authors: J
authors: Wang
authors: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors: Fang
authors: F
authors: Jin
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D010513:Periodontal Ligament / Q000378:metabolism* / Q000473:pathology; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology