title: Derivation of LIF-independent mouse iPS cells with modified Oct4.
abstract: It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.
authors: Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Hiroyuki
authors: H
authors: Hirai
authors: Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA; Division of Endocrinology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Meri
authors: M
authors: Firpo
authors: Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: kikyo001@umn.edu.
authors: Nobuaki
authors: N
authors: Kikyo
mesh_terms: D000818:Animals; D002460:Cell Line; D065150:Cellular Reprogramming; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D007621:Karyotyping; D016547:Kinesins / Q000235:genetics / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D053605:Leukemia Inhibitory Factor / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D053605:Leukemia Inhibitory Factor; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D055748:SOXB1 Transcription Factors; D050796:STAT3 Transcription Factor; C089655:Kif4 protein, mouse; D016547:Kinesins