title: A new strategy for site-specific alkylation of DNA using oligonucleotides containing an abasic site and alkylating probes.
abstract: Selective chemical reactions with DNA, such as its labelling, are very useful in many applications. In this paper, we discuss a new strategy for the selective alkylation of DNA using an oligonucleotide containing an abasic site and alkylating probes. We designed three probes consisting of 2-AVP as a reactive moiety and three kinds of binding moiety with high affinity to duplex DNA. Among these probes, Hoechst-AVP probe exhibited high selectivity and efficient reactivity to thymine bases at the site opposite an abasic site in DNA. Our method is potentially useful for inducing site-directed reactions aimed at inhibiting polymerase reactions.
authors: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, 980-8577, Japan. nagatugi@tagen.tohoku.ac.jp.
authors: Norihiro
authors: N
authors: Sato
authors: Genichiro
authors: G
authors: Tsuji
authors: Yoshihiro
authors: Y
authors: Sasaki
authors: Akira
authors: A
authors: Usami
authors: Takuma
authors: T
authors: Moki
authors: Kazumitsu
authors: K
authors: Onizuka
authors: Ken
authors: K
authors: Yamada
authors: Fumi
authors: F
authors: Nagatsugi
mesh_terms: D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015335:Molecular Probes; D009841:Oligonucleotides; D004247:DNA