title: Umbilical cord blood-derived dendritic cells infected by adenovirus for SP17 expression induce antigen-specific cytotoxic T cells against NSCLC cells.
abstract: Sperm protein 17 (SP17), a cancer/testis antigen, is expressed by non-small cell lung cancer (NSCLC). This study examined whether dendritic cells (DC) from human umbilical cord blood (UCB) could be induced for SP17 expression and induce antigen-specific CD8(+) cytotoxic T lymphocytes (CTLs) against NSCLC in vitro. We generated recombinant adenovirus of Ad-SP17 and control Ad-null. Infection with Ad-SP17, but not control, induced higher levels of SP17 expression in UCB-derived DC-Ad-SP17. Infection with Ad-SP17 significantly increased the frequency of CD80(+), CD83(+), CD86(+), and HLA-DR(+) DC that produced higher levels of IL-12, but lower IL-10. Co-culture of DC-Ad-SP17 with autologous UCB lymphocytes induced high frequency of IFNγ(+) CD8(+) CTLs, which had selective cytotoxicity against SP17(+) lung cancer CRL-5922 cells in a HLA-I restrictive manner. Thus, UCB-derived DC modulated for SP17 expression induced antigen-specific anti-tumor immunity against SP17(+) NSCLC, and SP17 may be a valuable target for development of immunotherapy against SP17(+) NSCLC.
authors: Molecular Oncology Laboratory of Cancer Research Institute, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors: Yang
authors: Y
authors: Liu
authors: Molecular Oncology Laboratory of Cancer Research Institute, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors: Xin
authors: X
authors: Tian
authors: Department of Rheumatology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors: Shenyi
authors: S
authors: Jiang
authors: Liaoning Maternal and Children Health Care Hospital, Shenyang 110005, China.
authors: Xuemei
authors: X
authors: Ren
authors: Liaoning Maternal and Children Health Care Hospital, Shenyang 110005, China.
authors: Fengjie
authors: F
authors: Liu
authors: Liaoning Maternal and Children Health Care Hospital, Shenyang 110005, China.
authors: Jichun
authors: J
authors: Yang
authors: Liaoning Maternal and Children Health Care Hospital, Shenyang 110005, China.
authors: Yanling
authors: Y
authors: Chen
authors: Molecular Oncology Laboratory of Cancer Research Institute, The First Affiliated Hospital of China Medical University, Shenyang 110001, China. Electronic address: youhongj19@hotmail.com.
authors: Youhong
authors: Y
authors: Jiang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000951:Antigens, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002148:Calmodulin-Binding Proteins; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000628:therapy; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis; D053765:Interleukin-12 Subunit p35 / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000276:immunology* / Q000628:therapy; D008565:Membrane Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; C508609:IL10 protein, human; C508858:IL12A protein, human; D053765:Interleukin-12 Subunit p35; D008565:Membrane Proteins; C090172:SPA17 protein, human; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Adenovirus; Dendritic cells; Non-small cell lung cancer; Sperm protein 17; Umbilical cord blood