title: Isolation and analysis of polysaccharide showing high hyaluronidase inhibitory activity in Nostochopsis lobatus MAC0804NAN.
abstract: An active substance with high hyaluronidase inhibitory effect was isolated from the edible cyanobacterium Nostochopsis lobatus MAC0804NAN strain and characterized. The active component in the hot water extract was purified by anion exchange and gel filtration chromatography and was found to be a polysaccharide. The IC(50) against hyaluronidase of the purified polysaccharide was 7.18 μg/ml whose inhibitory activity is 14.5 times stronger than that of disodium cromoglycate (DSCG), an anti-allergy medication. The carbohydrate composition which was analyzed by GC-MS and NMR was found to be composed mainly of glucose, glucuronic acid, fucose, 2-O-methylfucose, mannose, galactose and xylose.
authors: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; MAC Gifu Research Institute, MicroAlgae Corporation, 4-15 Akebono, Gifu 500-8148, Japan.
authors: Yuji
authors: Y
authors: Yamaguchi
authors: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: koketsu@gifu-u.ac.jp.
authors: Mamoru
authors: M
authors: Koketsu
mesh_terms: D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004205:Cromolyn Sodium / Q000494:pharmacology; D000458:Cyanobacteria / Q000201:enzymology*; D008401:Gas Chromatography-Mass Spectrometry; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D002241:Carbohydrates; D011134:Polysaccharides; D006821:Hyaluronoglucosaminidase; D004205:Cromolyn Sodium
keywords: Edible cyanobacteria; Hyaluronidase inhibitor; Methylfucose; Nostochopsis; Polysaccharide