title: Evaluation of Fluorescence Imaging with Reflectance Enhancement (FIRE) for Quantifying Enamel Demineralization In vitro.
abstract: OBJECTIVE
  To assess the ability of fluorescence imaging with reflectance enhancement (FIRE) to quantify early enamel demineralization in vitro.
  
  
  METHODS
  A total of 30 bovine enamel specimens were immersed in demineralizing solution to produce artificial caries. Specimens were examined by FIRE, quantitative light-induced fluorescence (QLF), and polarized light microscopy (PLM) at baseline and after 0.5, 1, 2, 4, 8, 24, 48 and 72 h of immersion. Fluorescence loss measured by FIRE and QLF was compared with lesion depth measurements by PLM.
  
  
  RESULTS
  Over all time points, measurement of fluorescence loss by FIRE correlated well with the measurement of x0394;F by QLF and of x0394;Q by QLF. Both FIRE and QLF techniques showed significant correlation with PLM.
  
  
  CONCLUSIONS
  FIRE technology may be useful for quantifying enamel demineralization in vitro.
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors: Qiang
authors: Q
authors: Xiao
authors: Rui
authors: R
authors: Tu
authors: Tao
authors: T
authors: He
authors: Wei
authors: W
authors: Yin
authors: Xue
authors: X
authors: Li
authors: Deyu
authors: D
authors: Hu
authors: Ximu
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D003731:Dental Caries / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D003743:Dental Enamel / Q000473:pathology*; D003804:Dentin / Q000473:pathology; D008859:Microscopy, Polarization; D061848:Optical Imaging / Q000379:methods*; D017001:Tooth Demineralization / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology