title: Analysis of small carbohydrates in several bioactive botanicals by gas chromatography with mass spectrometry and liquid chromatography with tandem mass spectrometry.
abstract: Bioactive botanicals contain natural compounds with specific biological activity, such as antibacterial, antioxidant, immune stimulating, and taste improving. A full characterization of the chemical composition of these botanicals is frequently necessary. A study of small carbohydrates from the plant materials of 18 bioactive botanicals is further described. The study presents the identification of the carbohydrate using a gas chromatographic-mass spectrometric analysis that allows detection of molecules as large as maltotetraose, after changing them into trimethylsilyl derivatives. A number of carbohydrates in the plant (fructose, glucose, mannose, sucrose, maltose, xylose, sorbitol, and myo-, chiro-, and scyllo-inositols) were quantitated using a novel liquid chromatography with tandem mass spectrometric technique. Both techniques involved new method developments. The gas chromatography with mass spectrometric analysis involved derivatization and separation on a Rxi(®)-5Sil MS column with H2 as a carrier gas. The liquid chromatographic separation was obtained using a hydrophilic interaction type column, YMC-PAC Polyamine II. The tandem mass spectrometer used an electrospray ionization source in multiple reaction monitoring positive ion mode with the detection of the adducts of the carbohydrates with Cs(+) ions. The validated quantitative procedure showed excellent precision and accuracy allowing the analysis in a wide range of concentrations of the analytes.
authors: R.J. Reynolds Tobacco Co, Winston-Salem, NC, USA.
authors: Serban
authors: S
authors: Moldoveanu
authors: R.J. Reynolds Tobacco Co, Winston-Salem, NC, USA.
authors: Wayne
authors: W
authors: Scott
authors: Eurofins Lancaster Laboratories, Inc, Lancaster, PA, USA.
authors: Jeff
authors: J
authors: Zhu
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002241:Carbohydrates
keywords: Bioactive botanicals; Hydrophilic interaction chromatography; Small carbohydrates