title: Metabolism study of boldenone in human urine by gas chromatography-tandem mass spectrometry.
abstract: Boldenone (BOLD), an anabolic steroid, is likely to be abused in livestock breeding and in sports. Although some of BOLD metabolites in human urine, such as 5β-adrost-1-en-17β-ol-3-one (BM1), have been detected, investigations on their excretion patterns for both genders are insufficient. Moreover, little research on 17α-BOLD glucuronide as a metabolite in human urine has been reported. The aim of this study is to make a contribution to the knowledge of 17β-BOLD metabolism in humans. Three male and three female volunteers were orally administrated with 30mg 17β-BOLD. Urine samples were collected and analyzed with gas chromatography-tandem mass spectrometry. The data proved that 17β-BOLD, BM1, and 17α-BOLD were excreted in urine in both free and glucuronic conjugated forms after administration of 17β-BOLD. For most subjects, the urinary concentrations of BM1 were higher than that of 17β-BOLD. 17α-BOLD was excreted in small amounts. 17α-BOLD, 17β-BOLD, and BM1 were present naturally in urine with low concentrations. Administration of 30mg 17β-BOLD could not influence the excretion profiles of urinary androsterone, etiocholanolone, and testosterone/epitestosterone ratio. There were no differences in BOLD metabolic patterns between man and woman.
authors: Kunming Medical University, Yunnan 650500, China.
authors: Xinchen
authors: X
authors: Wu
authors: Kunming Medical University, Yunnan 650500, China.
authors: Feng
authors: F
authors: Gao
authors: Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Wenxin
authors: W
authors: Zhang
authors: Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: jiannitcm@sina.com.
authors: Jian
authors: J
authors: Ni
mesh_terms: D000284:Administration, Oral; D000328:Adult; D045930:Anabolic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*; D001711:Biotransformation; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards; D020719:Glucuronides / Q000652:urine; D006801:Humans; D008297:Male; D015394:Molecular Structure; D065667:Renal Elimination; D012737:Sex Factors; D013739:Testosterone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000652:urine; D016482:Urinalysis; D055815:Young Adult
chemical_list: D045930:Anabolic Agents; D020719:Glucuronides; D013739:Testosterone; C013915:boldenone
keywords: Boldenone; Gas chromatography–tandem mass spectrometry; Metabolism