title: The BOC ELISA, a ruminant-specific AMH immunoassay, improves the determination of plasma AMH concentration and its correlation with embryo production in cattle.
abstract: Plasma anti-Müllerian hormone (AMH) concentrations have been recently found to be predictive of the number of embryos recovered after FSH superovulatory treatment in the cow. However, the sensitivity of the Active Müllerian-inhibiting substance/AMH ELISA (ref. 10-14400; DSL-Beckman-Coulter) used to make these measurements in bovine plasma samples is low because it was developed to measure human AMH levels. To overcome this limitation, we developed an immunoassay specific for the bovine (B), ovine (O), and caprine (C) species, the bovine-ovine-caprine (BOC) ELISA. For this purpose, we produced recombinant bovine AMH for standardization, and we used monoclonal antibodies raised against bovine AMH, previously prepared by our laboratory. We evaluated the precision, accuracy, specificity, limit of detection, and functional sensitivity of the assay. The intra-assay coefficient of variation ranged between 3.4% and 11.3% for AMH concentrations between 23.68 and 1.74 ng/mL, and the interassay coefficient of variation ranged between 4.8% and 20.5% for concentrations between 25.53 and 1.42 ng/mL, respectively. The assay displayed a good linearity, had a detection limit of 0.4 ng/mL and a functional sensitivity of 1.4 ng/mL. It also cross-reacted with ovine and caprine AMHs. Both the mean and median AMH levels measured in 40 cow plasma samples using the BOC ELISA were approximately 44 fold higher than the mean and median AMH levels measured with the Active Müllerian-inhibiting substance/AMH ELISA. Moreover, a higher correlation was observed between the average number of embryos recovered from each cow after superovulatory treatment and AMH concentrations measured with the BOC ELISA. This BOC ELISA provides a very efficient tool for evaluating the ovarian follicular reserve of cows and predicting their embryo production capacity.
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Nassim
authors: N
authors: Arouche
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Jean-Yves
authors: JY
authors: Picard
authors: INRA, UMR85, Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37041 Tours, France; IFCE, F-37041 Nouzilly, France.
authors: Danielle
authors: D
authors: Monniaux
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Soazik P
authors: SP
authors: Jamin
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Bernard
authors: B
authors: Vigier
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Nathalie
authors: N
authors: Josso
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Richard L
authors: RL
authors: Cate
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France. Electronic address: nathalie.diclemente-besse@univ-paris-diderot.fr.
authors: Nathalie
authors: N
authors: di Clemente
authors: Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France; INSERM U1133, Physiologie de l'Axe Gonadotrope, F-75013 Paris, France; CNRS, UMR 8251, Biologie Fonctionnelle et Adaptative, F-75013 Paris, France.
authors: Joëlle
authors: J
authors: Taieb
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood*; D002417:Cattle; D047108:Embryonic Development; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D010050:Ovarian Function Tests / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity
chemical_list: D054304:Anti-Mullerian Hormone
keywords: Anti-Müllerian hormone; Bovine; Immunoassay; Müllerian-inhibiting substance