title: Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search.
abstract: Broad screening and identification of β-agonists in feed, serum, urine, muscle and liver samples was achieved in a quick and highly sensitive manner using ultra high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) combined with a spectra library search. Solid-phase extraction technology was employed for sample purification and enrichment. After extraction and purification, the samples were analyzed using a Q-Orbitrap high-resolution mass spectrometer under full-scan and data-dependent MS/MS mode. The acquired mass spectra were compared with an in-house library (compound library and MS/MS mass spectral library) built with TraceFinder Software which contained the M/Z of the precursor ion, chemical formula, retention time, character fragment ions and the entire MS/MS spectra of 32 β-agonist standards. Screening was achieved by comparing 5 key mass spectral results and positive matches were marked. Using the developed method, the identification results from 10 spiked samples and 238 actual samples indicated that only 2% of acquired mass spectra produced false identities. The method validation results showed that the limit of detection ranged from 0.021-3.854 μg kg(-1)and 0.015-1.198 ng mL(-1) for solid and liquid samples, respectively.
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
authors: Tingting
authors: T
authors: Li
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Jingjing
authors: J
authors: Cao
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Zhen
authors: Z
authors: Li
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
authors: Xian
authors: X
authors: Wang
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China. Electronic address: hepingli@cau.edu.cn.
authors: Pingli
authors: P
authors: He
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000493:pharmacokinetics; D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012756:Sheep; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods; D014018:Tissue Distribution
chemical_list: D000318:Adrenergic beta-Agonists
keywords: Animal body fluid and tissues; Bambuterol (PubChem CID: 54766); Bamethan (PubChem CID: 2292); Brombuterol (PubChem CID: 3084849); Cimaterol (PubChem CID: 2755); Cimbuterol (PubChem CID: 3027547); Clenbuterol (PubChem CID: 2783); Clenisopenterol (PubChem CID: 71312595); Clenproperol (PubChem CID: 217246); Clorprenaline (PubChem CID: 2810); Dobutamine (PubChem CID: 36811); Epinephrine (PubChem CID: 5816); Fenoterol (PubChem CID: 3343); Formoterol (PubChem CID: 3410); IUPCA name: 2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol); Identification; Isoetharine (PubChem CID: 3762); Isoprenaline (PubChem CID: 3779); Isoxsuprine (PubChem CID: 3783); Labetalol (PubChem CID: 3869); Mabuterol (PubChem CID: 3995); Mapenterol hydrochloride (PubChem CID: 13355612); Molecular Formula: C(19)H(24)N(2)O(4); Orciprenaline (PubChem CID: 4086); Penbutolol (PubChem CID: 37464); Phenylethanolamine A (Molecular Weight: 344.17306; Pirbuterol (PubChem CID: 4845); Procaterol (PubChem CID: 4916); Ractopamine (PubChem CID: 56052); Ritodrine (PubChem CID: 33572); Salbutamol (PubChem CID: 2083); Salmeterol (PubChem CID: 5152); Spectra library; Terbutaline (PubChem CID: 5403); Tulobuterol (PubChem CID: 5606); UHPLC-Q-Orbitrap HRMS; Zilpaterol (PubChem CID: 3086576); Zinterol (PubChem CID: 37990); β-Agonists