title: Hydrogen peroxide causes Vibrio vulnificus bacteriolysis accelerated by sulfonyl fluoride compounds.
abstract: Induction of bacteriolysis of Vibrio vulnificus cells by 10 mM hydrogen peroxide (H(2)O(2)) was analyzed. All Vibrio species examined, except for Vibrio hollisae, were lysed by 10 mM H(2)O(2). Bacteriophage induction was not the cause of H(2)O(2)-induced bacteriolysis. Autolysis is also known to cause bacteriolysis. VvpS protein is a serine protease of V. vulnificus essential for autolysis. vvpS mutant underwent H(2)O(2)-induced bacteriolysis in the same manner as the wild type. Protease inhibitors including serine protease inhibitors did not inhibit H(2)O(2)-induced bacteriolysis, which means that bacteriolysis is not due to autolysis. Unexpectedly, H(2)O(2)-induced bacteriolysis was accelerated by adding 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) and phenylmethylsulfonyl fluoride which are serine protease inhibitors. The hydroxyl radical was generated by H(2)O(2)-AEBSF interaction. It was considered that H(2)O(2)-induced bacteriolysis was caused by the hydroxyl radical which was generated by Fenton reaction, and possibly mediated by AEBSF. Deferoxamine, an agent chelating ferric ion and Fenton reaction inhibitor, suppressed both H(2)O(2)-induced bacteriolysis and its acceleration by AEBSF. This suggests that both phenomena were Fenton reaction dependent, and hydroxyl radical generated by Fenton reaction caused bacteriolysis of V. vulnificus though the reason for high susceptibility of Vibrio species to hydroxyl radical is not known.
authors: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. myoshimura@fukuoka-u.ac.jp.|Division of Medical Oncology, Hematology and Infectious Disease, Department of Internal Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan, Fukuoka, 814-0180, Japan. myoshimura@fukuoka-u.ac.jp.
authors: Michinobu
authors: M
authors: Yoshimura
authors: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors: Tetsuro
authors: T
authors: Tamura
authors: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors: Ken-ichiro
authors: K
authors: Iida
authors: Department of Oral Health, Growth and Development, Division of Oral Infectious Diseases and Immunology,Graduate School of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors: Susumu
authors: S
authors: Shiota
authors: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors: Hiroaki
authors: H
authors: Nakayama
authors: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors: Shin-ichi
authors: S
authors: Yoshida
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001433:Bacteriolysis / Q000187:drug effects*; D003676:Deferoxamine / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry; D017262:Siderophores / Q000494:pharmacology; D013441:Sulfinic Acids / Q000494:pharmacology*; D041261:Vibrio vulnificus / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D017262:Siderophores; D013441:Sulfinic Acids; D017665:Hydroxyl Radical; C048899:sulfuryl fluoride; D006861:Hydrogen Peroxide; D003676:Deferoxamine
keywords: Bacteriolysis; Fenton reaction; Hydrogen peroxide; Hydroxyl radical; Sulfonyl fluoride compounds; Vibrio vulnificus