title: Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015).
abstract: One major obstacle to the routine deployment of stem cell-derived cells is their instability in culture. On page 1820 David C. Hay and co-workers describe the use of a synthetic polymer surface. The image shows stem cell-derived hepatocytes replated on this polyurethane surface. Importantly the cells express Zonal Occludin (green stain) at the cell surface, which indicates that the cells display elements of polarization. The blue stain is DAPI, which demarks the nucleus.
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: Baltasar Lucendo
authors: BL
authors: Villarin
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: Kate
authors: K
authors: Cameron
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: Dagmara
authors: D
authors: Szkolnicka
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: Hassan
authors: H
authors: Rashidi
authors: Faculty of Life Sciences, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK.
authors: Nicola
authors: N
authors: Bates
authors: Faculty of Life Sciences, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK.
authors: Susan J
authors: SJ
authors: Kimber
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: Oliver
authors: O
authors: Flint
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: Stuart J
authors: SJ
authors: Forbes
authors: MRC Centre for Inflammation Research, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
authors: John P
authors: JP
authors: Iredale
authors: School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3FJ, UK.
authors: Mark
authors: M
authors: Bradley
authors: MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
authors: David C
authors: DC
authors: Hay
keywords: differentiation; gene signature; hepatocytes; pluripotent stem cells; polymers