title: Synthesis and characterization of novel molecularly imprinted polymer - coated Mn-doped ZnS quantum dots for specific fluorescent recognition of cocaine.
abstract: Mn-doped ZnS quantum dots (QDs) coated with a molecularly imprinted polymer (MIP) material selective toward cocaine and its metabolites have been prepared and applied to cocaine (COC) and metabolites assessment by spectrofluorimetry. Ultrasound irradiation (37kHz) was novelty used for performing the Mn-doped ZnS QDs synthesis as well as for preparing the QD based MIP-coated composite by precipitation polymerization (imprinting process). This fact allowed the synthesis to be accomplished in four hours. In addition, the use of ultrasound irradiation during MIP-QDs synthesis increased the homogeneity of the QDs size, and reduced nanoparticles agglomeration. MIP was synthesized using COC as a template molecule, ethylene dimethacrylate (EDMA) as a functional monomer, divinylbenzene (DVB) as a cross-linker, and 2,2'-azobisisobutyronitrile (AIBN) as an initiator. The fluorescence of MIP-coated QDs was quenched by the template (COC) and also by metabolites from COC such as benzoylecgonine (BZE), and ecgonine methyl ester (EME). Quenching was not observed when performing experiments with non-imprinted polymer (NIP)-coated QDs; and also, fluorescence quenching of MIP-coated QDs was not observed by other drugs of abuse and metabolites (heroin and cannabis abuse). This fact indicates that the prepared material recognize only COC (template) and metabolites.
authors: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n. 15782 Santiago de Compostela, Spain.
authors: María Pilar
authors: MP
authors: Chantada-Vázquez
authors: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n. 15782 Santiago de Compostela, Spain.
authors: Juan
authors: J
authors: Sánchez-González
authors: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n. 15782 Santiago de Compostela, Spain.
authors: Elena
authors: E
authors: Peña-Vázquez
authors: Department of Pathologic Anatomy and Forensic Sciences, Faculty of Medicine, University of Santiago de Compostela, Rúa de San Francisco, s/n. 15782 Santiago de Compostela, Spain.
authors: María Jesús
authors: MJ
authors: Tabernero
authors: Department of Pathologic Anatomy and Forensic Sciences, Faculty of Medicine, University of Santiago de Compostela, Rúa de San Francisco, s/n. 15782 Santiago de Compostela, Spain.
authors: Ana María
authors: AM
authors: Bermejo
authors: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n. 15782 Santiago de Compostela, Spain.
authors: Pilar
authors: P
authors: Bermejo-Barrera
authors: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n. 15782 Santiago de Compostela, Spain. Electronic address: antonio.moreda@usc.es.
authors: Antonio
authors: A
authors: Moreda-Piñeiro
mesh_terms: D015374:Biosensing Techniques; D003042:Cocaine / Q000302:isolation & purification*; D006801:Humans; D008345:Manganese / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D011108:Polymers; D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; D003042:Cocaine; C031238:zinc sulfide; D013691:Tellurium
keywords: Cocaine; Mn-doped ZnS quantum dot; Molecularly imprinted polymer; Spectrofluorimetry