title: Transcriptome analysis to identify genes for peptides and proteins involved in immunity and reproduction from male accessory glands and ejaculatory duct of Bactrocera dorsalis.
abstract: In the male reproductive system of insects, the male accessory glands and ejaculatory duct (MAG/ED) are important organs and their primary function is to enhance the fertility of spermatozoa. Proteins secreted by the MAG/ED are also known to induce post-mating changes and immunity responses in the female insect. To understand the gene expression profile in the MAG/ED of the oriental fruit fly Bactrocera dorsalis (Hendel), that is an important pest in fruits, we performed an Illumina-based deep sequencing of mRNA. This yielded 54,577,630 clean reads corresponding to 4.91Gb total nucleotides that were assembled and clustered to 30,669 unigenes (average 645bp). Among them, 20,419 unigenes were functionally annotated to known proteins/peptides in Gene Orthology, Clusters of Orthologous Groups, Kyoto Encyclopedia of Genes and Genomes pathway databases. Typically, many genes were involved in immunity and these included microbial recognition proteins and antimicrobial peptides. Subsequently, the inducible expression of these immunity-related genes was confirmed by qRT-PCR analysis when insects were challenged with immunity-inducible factors, suggesting their function in guaranteeing fertilization success. Besides, we identified some important reproductive genes such as juvenile hormone- and ecdysteroid-related genes in this de novo assembly. In conclusion, this transcriptomic sequencing of B. dorsalis MAG/ED provides insights to facilitate further functional research of reproduction, immunity and molecular evolution of reproductive proteins in this important agricultural pest.
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Dong
authors: D
authors: Wei
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Chuan-Bei
authors: CB
authors: Tian
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Shi-Huo
authors: SH
authors: Liu
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Tao
authors: T
authors: Wang
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; Department of Crop Protection, Ghent University, B-9000 Ghent, Belgium.
authors: Guy
authors: G
authors: Smagghe
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Fu-Xian
authors: FX
authors: Jia
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Wei
authors: W
authors: Dou
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China. Electronic address: jjwang7008@yahoo.com.
authors: Jin-Jun
authors: JJ
authors: Wang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004440:Ecdysone / Q000096:biosynthesis / Q000235:genetics; D004543:Ejaculatory Ducts / Q000502:physiology; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005837:Genitalia, Male / Q000502:physiology*; D059014:High-Throughput Nucleotide Sequencing; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism; D008297:Male; D058977:Molecular Sequence Annotation; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D033621:Tephritidae / Q000235:genetics / Q000276:immunology / Q000502:physiology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D004798:Enzymes; D019476:Insect Proteins; D007605:Juvenile Hormones; D010455:Peptides; D004440:Ecdysone
keywords: Antimicrobial peptide; Bactrocera dorsalis; Immunity; Male accessory glands; Reproduction; Transcriptome