title: Impact of tributyltin on antioxidant and DNA damage response in spermatozoa of freshwater prawn Macrobrachium rosenbergii.
abstract: Effects of tributyltin (TBT) on antioxidant [total superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR)] and DNA damage levels in the spermatozoa were studied and reported here for the first time in the freshwater prawn Macrobrachium rosenbergii. Three groups of (n = 10 in each group) fishes were exposed to three different nominal concentrations of TBT viz., 1, 2, and 4 mg L(-1) along with control group for 90 days. Significant decrease of antioxidant and increased DNA damage levels were seen at higher doses of 2 and 4 mg L(-1). In prawn, the antioxidant level plays a vital role in sperm protection, activation, differential functions related to the physiology, and reproductive behavior. This study serves as a biomonitoring tool to assess the TBT effects on reproductive behavior of aquatic biota.
authors: Department of Biotechnology, Sri Sankara Arts and Science College, Kancheepuram, Tamil Nadu, India.
authors: K Umaa
authors: KU
authors: Rani
authors: P.G. & Research Department of Zoology, The New College, Chennai, 600014, Tamil Nadu, India. saiyad_musthafa@rediffmail.com.
authors: M Saiyad
authors: MS
authors: Musthafa
authors: P.G. & Research Department of Zoology, The New College, Chennai, 600014, Tamil Nadu, India.
authors: Mehrajuddin
authors: M
authors: War
authors: Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.
authors: Mohammad K
authors: MK
authors: Al-Sadoon
authors: Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.
authors: Bilal Ahmad
authors: BA
authors: Paray
authors: Department of Chemistry, The New College, Chennai, 600014, Tamilnadu, India.
authors: T H Mohamed Ahadhu
authors: TH
authors: Shareef
authors: P.G. & Research Department of Zoology, Periyar E.V.R College of Arts and Science, Trichirappalli, Tamil Nadu, India.
authors: P Mohideen Askar
authors: PM
authors: Nawas
mesh_terms: D000818:Animals; D004249:DNA Damage; D005618:Fresh Water; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008297:Male; D033541:Palaemonidae / Q000187:drug effects* / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Comet assay; GPx: GR; SOD; Spermatozoa; TBT