title: Experimental and theoretical investigation effect of flavonols antioxidants on DNA damage.
abstract: A new electrochemical biosensor was developed to demonstrate the effect of Acridine Orange (AO) on DNA damage. Then, the biosensor was used to check the inhibitors effect of three flavonols antioxidants (myricetin, fisetin and kaempferol) on DNA damage. Acridine Orange (AO) was used as a damaging agent because it shows a high affinity to nucleic acid and stretch of the double helical structure of DNA. Decreasing on the oxidation signals of adenine and guanine (in the DNA) in the presence of AO were used as probes to study the antioxidants power, using DNA-modified screen printed graphene electrode (DNA/SPGE). The results of our study showed that the DNA-biosensor could be suitable biosensor to investigate the inhibitors ability of the flavonols antioxidants on the DNA damage. The linear dependency was detected in the two regions in the ranges of 1.0-15.0 and 15.0-500.0 pmol L(-1). The detection limit was found 0.5 pmol L(-1) and 0.6 pmol L(-1) for guanine and adenine, respectively. To confirm the electrochemical results, Uv-Vis and fluorescence spectroscopic methods were used too. Finally molecular dynamic (MD) simulation was performed on the structure of DNA in a water box to study any interaction between the antioxidant, AO and DNA.
authors: Department of Analytical Chemistry, Faculty of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: Ensafi@cc.iut.ac.ir.
authors: Ali A
authors: AA
authors: Ensafi
authors: Department of Analytical Chemistry, Faculty of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: E
authors: E
authors: Heydari-Soureshjani
authors: Department of Analytical Chemistry, Faculty of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: M
authors: M
authors: Jafari-Asl
authors: Department of Analytical Chemistry, Faculty of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: B
authors: B
authors: Rezaei
authors: Drug Design in Silico Lab., Chemistry Faculty, K. N. Toosi University of Technology, Tehran, Iran.
authors: Jahan B
authors: JB
authors: Ghasemi
authors: Drug Design in Silico Lab., Chemistry Faculty, K. N. Toosi University of Technology, Tehran, Iran.
authors: Elham
authors: E
authors: Aghaee
mesh_terms: D000165:Acridine Orange / Q000633:toxicity*; D000225:Adenine / Q000032:analysis; D000975:Antioxidants / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004249:DNA Damage / Q000187:drug effects*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D044948:Flavonols / Q000494:pharmacology*; D006108:Graphite / Q000737:chemistry; D006147:Guanine / Q000032:analysis; D057230:Limit of Detection; D062105:Molecular Docking Simulation; D009690:Nucleic Acid Conformation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D044948:Flavonols; D006147:Guanine; D006108:Graphite; D004247:DNA; D000165:Acridine Orange; D000225:Adenine
keywords: DNA damage; Flavonols anti-oxidants; Graphene modified screen printed electrode; Molecular docking; Voltammetry