title: Dynamical properties of gene regulatory networks involved in long-term potentiation.
abstract: The long-lasting enhancement of synaptic effectiveness known as long-term potentiation (LTP) is considered to be the cellular basis of long-term memory. LTP elicits changes at the cellular and molecular level, including temporally specific alterations in gene networks. LTP can be seen as a biological process in which a transient signal sets a new homeostatic state that is "remembered" by cellular regulatory systems. Previously, we have shown that early growth response (Egr) transcription factors are of fundamental importance to gene networks recruited early after LTP induction. From a systems perspective, we hypothesized that these networks will show less stable architecture, while networks recruited later will exhibit increased stability, being more directly related to LTP consolidation. Using random Boolean network (RBN) simulations we found that the network derived at 24 h was markedly more stable than those derived at 20 min or 5 h post-LTP. This temporal effect on the vulnerability of the networks is mirrored by what is known about the vulnerability of LTP and memory itself. Differential gene co-expression analysis further highlighted the importance of the Egr family and found a rapid enrichment in connectivity at 20 min, followed by a systematic decrease, providing a potential explanation for the down-regulation of gene expression at 24 h documented in our preceding studies. We also found that the architecture exhibited by a control and the 24 h LTP co-expression networks fit well to a scale-free distribution, known to be robust against perturbations. By contrast the 20 min and 5 h networks showed more truncated distributions. These results suggest that a new homeostatic state is achieved 24 h post-LTP. Together, these data present an integrated view of the genomic response following LTP induction by which the stability of the networks regulated at different times parallel the properties observed at the synapse.
authors: Department of Computer Science, University of Otago Dunedin, New Zealand ; Brain Health Research Centre, University of Otago Dunedin, New Zealand.
authors: Gonzalo S
authors: GS
authors: Nido
authors: Brain Health Research Centre, University of Otago Dunedin, New Zealand ; Department of Anatomy, Otago School of Medical Sciences, University of Otago Dunedin, New Zealand.
authors: Margaret M
authors: MM
authors: Ryan
authors: Department of Computer Science, University of Otago Dunedin, New Zealand ; Brain Health Research Centre, University of Otago Dunedin, New Zealand.
authors: Lubica
authors: L
authors: Benuskova
authors: Brain Health Research Centre, University of Otago Dunedin, New Zealand ; Department of Anatomy, Otago School of Medical Sciences, University of Otago Dunedin, New Zealand.
authors: Joanna M
authors: JM
authors: Williams
keywords: co-expression analysis; dynamic stability; gene expression; long-term potentiation; maintenance; memory; synaptic plasticity