title: Loss of the Capsule Increases the Adherence Activity but Decreases the Virulence of Avibacterium paragallinarum.
abstract: Avibacterium paragallinarum is the causative agent of infectious coryza, an important respiratory disease of chickens. The capsule is an important virulence determinant of many pathogenic bacteria, but the function of the capsule in Av. paragallinarum is not well defined. In this study, acapsular mutants of Av. paragallinarum were constructed by inactivation of the hctA gene using the TargeTron gene knockout system. The acapsular mutants were found to have greater hemagglutination activity than did the wild-type strain. Further, acapsular mutants exhibited an increased ability to adhere to DF-1 cells and to form biofilms on abiotic surfaces. Virulence assays showed that acapsular mutants were less virulent than the wild-type strain. Taken together, these results indicated that loss of capsule increases hemagglutination and adhesion activities but decreases the virulence of Av. paragallinarum. These results could be valuable to further elucidate the function of the capsule and the mechanism of pathogenicity of Av. paragallinarum.
authors: Tzu-Yi
authors: TY
authors: Tu
authors: Ming-Kun
authors: MK
authors: Hsieh
authors: Duen-Huey
authors: DH
authors: Tan
authors: Shan-Chia
authors: SC
authors: Ou
authors: Jui-Hung
authors: JH
authors: Shien
authors: Ting-Ying
authors: TY
authors: Yen
authors: Poa-Chun
authors: PC
authors: Chang
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D016667:Bacterial Capsules / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015661:Pasteurellaceae / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins