title: Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo.
abstract: In mammals, olfactory perception is based on the combinatorial activation of G protein-coupled receptors. Identifying the full repertoire of receptors activated by a given odorant in vivo, a quest that has been hampered for over 20 years by technical difficulties, would represent an important step in deciphering the rules governing chemoperception. We found that odorants induced a fast and reversible concentration-dependent decrease in the transcription of genes corresponding to activated receptors in intact mice. On the basis of this finding, we developed a large-scale transcriptomic approach to uncover receptor-ligand pairs in vivo. We identified the mouse and rat odorant receptor signatures corresponding to specific odorants. Finally, we found that this approach, which can be used for species for which no genomic sequence is available, is also applicable to non-vertebrate species such as Drosophila.
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Benoît
authors: B
authors: von der Weid
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Daniel
authors: D
authors: Rossier
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Matti
authors: M
authors: Lindup
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Joël
authors: J
authors: Tuberosa
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Alexandre
authors: A
authors: Widmer
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Julien Dal
authors: JD
authors: Col
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Chenda
authors: C
authors: Kan
authors: Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Department of Basic Neurosciences, School of Medicine, University of Geneva, Geneva, Switzerland.
authors: Alan
authors: A
authors: Carleton
authors: Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.|Geneva Neuroscience Center, University of Geneva, Geneva, Switzerland.|Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva, Switzerland.
authors: Ivan
authors: I
authors: Rodriguez
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D018035:Receptors, Odorant / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012903:Smell / Q000502:physiology*
chemical_list: D018035:Receptors, Odorant