title: Two New Species of Liuixalus (Rhacophoridae, Anura): Evidence from Morphological and Molecular Analyses.
abstract: Due to small body sizes, superficial similarities in morphologies, and obscure activity behaviors, the phylogeny and taxonomy of species in the genus Liuixalus were very troublesome. Some species might comprise a complex of cryptic species. To investigate the species of group, we constructed the matrilineal genealogy of the genus using 16s rRNA mitochondrial DNA sequences. Analyses recovered six well supported matrilines that involved L. romeri, L. ocellatus, L. hainanus, L. calcarius, Liuixalus shiwandashan sp. nov. and Liuixalus jinxiuensis sp. nov., though the historical relationships among them remained unresolved. Currently, Liuixalus included 4 species, distributed eastwards from northern Vietnam to Hong Kong, China. Based on genealogical and morphological distinctiveness, we described Liuixalus jinxiuensis sp. nov. from the type locality Mt. Dayao, Jinxiu, China and Liuixalus shiwandashan sp. nov. from the type locality Mt. Shiwanda, China. A combination of morphological measurements, genetic, bioacoustic and osteological analysis was served to diagnose the new taxa.
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China; College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
authors: Shaobo
authors: S
authors: Qin
authors: Natural History Museum of Guangxi, Nanning, Guangxi, China.
authors: Yunming
authors: Y
authors: Mo
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China.
authors: Ke
authors: K
authors: Jiang
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China.
authors: Bo
authors: B
authors: Cai
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China.
authors: Feng
authors: F
authors: Xie
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China.
authors: Jianping
authors: J
authors: Jiang
authors: Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, Toronto, Ontario, Canada.
authors: Robert W
authors: RW
authors: Murphy
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China.
authors: Jia-Tang
authors: JT
authors: Li
authors: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China.
authors: Yuezhao
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D001001:Anura / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S