title: Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and molecular approaches.
abstract: We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) for the identification of 97 Corynebacterium clinical in comparison to identification strains by Api Coryne and MALDI-TOF-MS using 16S rRNA gene and hypervariable region of rpoB genes sequencing as a reference method. C. striatum was the predominant species isolated followed by C. amycolatum. There was an agreement between Api Coryne strips and MALDI-TOF-MS identification in 88.65% of cases. MALDI-TOF-MS was unable to differentiate C. aurimucosum from C. minutissimum and C. minutissimum from C. singulare but reliably identify 92 of 97 (94.84%) strains. Two strains remained incompletely identified to the species level by MALDI-TOF-MS and molecular approaches. They belonged to Cellulomonas and Pseudoclavibacter genus. In conclusion, MALDI-TOF-MS is a rapid and reliable method for the identification of Corynebacterium species. However, some limits have been noted and have to be resolved by the application of molecular methods.
authors: UR12/SP34 laboratoire de microbiologie et immunologie, CHU Farhat-Hached, Sousse, Tunisia; Faculté des sciences de Bizerte, université Carthage, Jarzouna Tunisia. Electronic address: alibi_sana@hotmail.fr.
authors: S
authors: S
authors: Alibi
authors: UR12/SP34 laboratoire de microbiologie et immunologie, CHU Farhat-Hached, Sousse, Tunisia.
authors: A
authors: A
authors: Ferjani
authors: UR12/SP34 laboratoire de microbiologie et immunologie, CHU Farhat-Hached, Sousse, Tunisia.
authors: O
authors: O
authors: Gaillot
authors: UR12/SP34 laboratoire de microbiologie et immunologie, CHU Farhat-Hached, Sousse, Tunisia.
authors: M
authors: M
authors: Marzouk
authors: Institut de microbiologie, centre hospitalier universitaire de Lille, 59037 Lille cedex, France.
authors: R
authors: R
authors: Courcol
authors: UR12/SP34 laboratoire de microbiologie et immunologie, CHU Farhat-Hached, Sousse, Tunisia.
authors: J
authors: J
authors: Boukadida
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D040143:Cellulomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D008835:Micrococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011934:Reagent Strips; D021521:Ribotyping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011934:Reagent Strips; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
keywords: 16S rRNA gene sequencing; 16S rRNA; Api Coryne; Corynebacterium; Corynebacterium spp.; Identification; MALDI-TOF-MS; SM-MALDI-TOF; Séquençage; rpoB