title: Chimeric peptide constructs comprising linear B-cell epitopes: application to the serodiagnosis of infectious diseases.
abstract: Linear B-cell epitopes are ideal biomarkers for the serodiagnosis of infectious diseases. However, the long-predicted diagnostic value of epitopes has not been realized. Here, we demonstrated a method, diagnostic epitopes in four steps (DEIFS), that delivers a combination of epitopes for the serodiagnosis of infectious diseases with a high success rate. Using DEIFS for malaria, we identified 6 epitopes from 8 peptides and combined them into 3 chimeric peptide constructs. Along with 4 other peptides, we developed a rapid diagnostic test (RDT), which is able to differentiate Plasmodium falciparum (P. falciparum) from Plasmodium vivax (P. vivax) infections with 95.6% overall sensitivity and 99.1% overall specificity. In addition to applications in diagnosis, DEIFS could also be used in the diagnosis of virus and bacterium infections, discovery of vaccine candidates, evaluation of vaccine potency, and study of disease progression.
authors: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, P. R. China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors: Yudong
authors: Y
authors: Lu
authors: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, P. R. China.
authors: Zhong
authors: Z
authors: Li
authors: Suzhou SJ Biomaterials Co. Ltd. Suzhou, 215123, P. R. China.
authors: Huan
authors: H
authors: Teng
authors: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, P. R. China.
authors: Hongke
authors: H
authors: Xu
authors: Suzhou SJ Biomaterials Co. Ltd. Suzhou, 215123, P. R. China.
authors: Songnan
authors: S
authors: Qi
authors: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P. R. China.
authors: Jian'an
authors: J
authors: He
authors: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P. R. China.
authors: Dayong
authors: D
authors: Gu
authors: Key Laboratory of Zoonosis, Jilin University, Changchun, 130062, P. R. China.
authors: Qijun
authors: Q
authors: Chen
authors: Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, P. R. China.
authors: Hongwei
authors: H
authors: Ma
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D015203:Reproducibility of Results; D012698:Serologic Tests / Q000379:methods*
chemical_list: D015415:Biomarkers; D018985:Epitopes, B-Lymphocyte; D010455:Peptides