title: Arabidopsis HECATE genes function in phytohormone control during gynoecium development.
abstract: The fruit, which develops from the fertilised gynoecium formed in the innermost whorl of the flower, is the reproductive organ and one of the most complex structures of an angiosperm plant. Phytohormones play important roles during flower and fruit patterning, morphogenesis and growth, and there is emerging evidence for a cross-talk between different classes of plant hormones throughout these processes. Here, we show that the bHLH transcription factors HECATE 1 (HEC1), HEC2 and HEC3, which have previously been identified as essential components of transmitting tract formation, affect both auxin and cytokinin responses during reproductive tissue development. We find that HEC1 interacts with SPATULA (SPT) to control carpel fusion and that both transcription factors restrict sensitivity to cytokinin in the gynoecium. In addition, HEC1 is tightly integrated into the auxin-signalling network at the levels of biosynthesis, transport and transcriptional response. Based on this data, we propose that HEC1 acts as a local modulator of auxin and cytokinin responses to control gynoecium development in Arabidopsis.
authors: Department of Stem Cell Biology, University of Heidelberg, Heidelberg D-69120, Germany christoph.schuster@slcu.cam.ac.uk jlohmann@meristemania.org.
authors: Christoph
authors: C
authors: Schuster
authors: Department of Stem Cell Biology, University of Heidelberg, Heidelberg D-69120, Germany.
authors: Christophe
authors: C
authors: Gaillochet
authors: Department of Stem Cell Biology, University of Heidelberg, Heidelberg D-69120, Germany christoph.schuster@slcu.cam.ac.uk jlohmann@meristemania.org.
authors: Jan U
authors: JU
authors: Lohmann
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D002450:Cell Communication / Q000502:physiology; D047369:Chromatin Immunoprecipitation; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D035264:Flowers / Q000254:growth & development* / Q000378:metabolism; D005638:Fruit / Q000254:growth & development* / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric; D014157:Transcription Factors / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; C587998:HECATE1 protein, Arabidopsis; D010937:Plant Growth Regulators; D014157:Transcription Factors
keywords: Arabidopsis; Auxin; Cytokinin; Gynoecium development; HECATE; Phytohormones; SPATULA