title: Rice α-fucosidase active against plant complex type N-glycans containing Lewis a epitope: purification and characterization.
abstract: Rice α-fucosidase (α-fucosidase Os, 58 kDa) that is active for α1-4 fucosyl linkage in Lewis a unit of plant N-glycans was purified to homogeneity. α-fucosidase Os showed activity against α1-3 fucosyl linkage in Lacto-N-fucopentaose III but not α1-3 fucosyl linkage in the core of plant N-glycans. The N-terminal sequence of α-fucosidase Os was identified as A-A-P-T-P-P-P-L-, and this sequence was found in the amino acid sequence of the putative rice α-fucosidase 1 (Os04g0560400).
authors: a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.|b Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission , Dhaka , Bangladesh.
authors: Md Ziaur
authors: MZ
authors: Rahman
authors: c Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology , Okayama University , Okayama , Japan.
authors: Makoto
authors: M
authors: Fujishige
authors: a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.|c Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology , Okayama University , Okayama , Japan.
authors: Megumi
authors: M
authors: Maeda
authors: a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.|c Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology , Okayama University , Okayama , Japan.
authors: Yoshinobu
authors: Y
authors: Kimura
mesh_terms: D000595:Amino Acid Sequence; D000606:Amino Sugars / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D007700:Kinetics; D007983:Lewis Blood Group Antigens; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D012275:Oryza / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D005644:alpha-L-Fucosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000606:Amino Sugars; D000939:Epitopes; D007983:Lewis Blood Group Antigens; C494219:Lewis a oligosaccharide; D009844:Oligosaccharides; D010940:Plant Proteins; D011134:Polysaccharides; C434666:lacto-N-fucopentaose III; D005644:alpha-L-Fucosidase
keywords: N-glycan degradation; Oryza sativa; plant N-glycan; α-fucosidase