title: Alteromonas confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring.
abstract: A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, DSSK2-12T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain DSSK2-12T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DSSK2-12T fell within the clade comprising the type strains of species of the genus Alteromonas. Strain DSSK2-12T exhibited 16S rRNA gene sequence similarity values of 97.2–98.1 % to the type strains of Alteromonas litorea, Alteromonas marina, Alteromonas hispanica and Alteromonas genovensis and of 95.39–96.98 % to those of other species of the genus Alteromonas. Strain DSSK2-12T contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c as the major fatty acids. The major polar lipids of strain DSSK2-12T were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain DSSK2-12T was 48.6 mol% and its mean DNA–DNA relatedness values with the type strains of A. litorea, A. marina, A. hispanica and A. genovensis were 9–21 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain DSSK2-12T is separated from other species of the genus Alteromonas. On the basis of the data presented, strain DSSK2-12T is considered to represent a novel species of the genus Alteromonas, for which the name Alteromonas confluentis sp. nov. is proposed. The type strain is DSSK2-12T ( = KCTC 42603T = CECT 8870T).
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangangu, Suwon, South Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangangu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors: Chul-Hyung
authors: CH
authors: Kang
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangangu, Suwon, South Korea.
authors: Sung-Min
authors: SM
authors: Won
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangangu, Suwon, South Korea.
authors: Ji-Min
authors: JM
authors: Park
authors: Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.
authors: Byung-Chan
authors: BC
authors: Kim
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangangu, Suwon, South Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D020591:Alteromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine