title: Identification of TRIM22 as a progesterone-responsive gene in Ishikawa endometrial cancer cells.
abstract: Progesterone plays important roles in implantation and maintains pregnancy. It antagonizes estrogen-mediated cell proliferation and promotes differentiation in the uterus. The action of progesterone is mediated by specific receptors, namely, the progesterone receptors (PRs). We generated two Ishikawa cell clones stably expressing PR isoform A (PR-A) and identified progesterone-responsive genes using cDNA microarray analysis. Fifteen genes were identified as progesterone-responsive gene candidates by microarray analysis and their progesterone-responsiveness was shown by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis. Out of these 15 genes, we focused on TRIM22. A database search revealed a progesterone response element (PRE) located from the -25 to -11 bp region upstream of TRIM22 exon 1. This PRE had a 1-bp mismatch in the consensus PRE sequence. A chromatin immunoprecipitation assay revealed that the interaction of PR with the TRIM22 PRE region increased in a hormone-dependent manner. The progesterone-dependent enhancer activity of TRIM22 PRE was demonstrated using a luciferase assay. Based on these results, we propose that TRIM22 is a direct target gene of PR and that it can mediate progesterone actions in uterine cells.
authors: Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Department of Obstetrics and Gynaecology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Mayuko
authors: M
authors: Saito-Kanatani
authors: Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Department of Anti-Aging Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Tomohiko
authors: T
authors: Urano
authors: Department of Obstetrics and Gynaecology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Hisahiko
authors: H
authors: Hiroi
authors: Department of Integrated Women's Health, St. Luke's International Hospital, 9-1 Akashi-cho, Chuo-ku, Tokyo 104-8560, Japan.
authors: Mikio
authors: M
authors: Momoeda
authors: Department of Obstetrics and Gynaecology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Masanori
authors: M
authors: Ito
authors: Department of Obstetrics and Gynaecology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Tomoyuki
authors: T
authors: Fujii
authors: Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Department of Anti-Aging Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Research Center for Genomic Medicine, Saitama Medical University, 1397-1, Yamane, Hidaka-shi, Saitama 350-1241, Japan. Electronic address: INOUE-GER@h.u-tokyo.ac.jp.
authors: Satoshi
authors: S
authors: Inoue
mesh_terms: D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D015778:Minor Histocompatibility Antigens; D011374:Progesterone / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011980:Receptors, Progesterone / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D000070584:Tripartite Motif Proteins
chemical_list: D015778:Minor Histocompatibility Antigens; D011980:Receptors, Progesterone; D012097:Repressor Proteins; C094314:TRIM22 protein, human; D000070584:Tripartite Motif Proteins; C101118:progesterone receptor A; D011374:Progesterone
keywords: Progesterone receptor; TRIM22; Uterine cells