title: Lentivirus-mediated shRNA interference of clusterin blocks proliferation, motility, invasion and cell cycle in the ovarian cancer cells.
abstract: BACKGROUND
  In a previous analysis on the patients with ovarian cancers, we have found that clusterin is a biomarker associated with ovarian cancer in vivo and may be a prognostic factor associated with adverse outcome. Here, we explored the effect of lentivirus-mediated shRNA interference of clusterin, investigated whether clusterin was associated with adverse outcome of ovarian cancer cells in vitro.
  
  
  METHODS
  OVCAR-3 and TOV-21G cell lines were infected with the lentivirus for delivering clusterin shRNA, and the stably transfected cells were selected. The effect of clusterin silencing was detected by western blotting assay. The proliferation, clonability, migration, invasion and cell cycle of two cell lines were detected separately by MTT assay, clone formation assay, scratch assay, transwell assay and fluorescence-activated cell sorting.
  
  
  RESULTS
  Following clusterin silencing with shRNA, the expression of clusterin in two cell lines were decreased. And the proliferation, clonability, migration, invasion of these two cell lines were down-regulated apparently. The cell cycle of two cell lines was disturbed, cells in G1 phase was increased, but cells in G2 and S phase was decreased.
  
  
  CONCLUSIONS
  The expression of clusterin is significantly correlated with the biological characteristics of ovarian cancer cells, it may be a potential molecular for ovarian cancer treatment.
authors: Department of Gynecology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. fuyx2005@163.com.
authors: Yanxia
authors: Y
authors: Fu
authors: Department of Pathology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, China. laiyr@126.com.
authors: Yingrong
authors: Y
authors: Lai
authors: Department of Pathology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, China. ljfengs@foxmail.com.
authors: Junfeng
authors: J
authors: Liu
authors: Department of Gynecology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 15322237338@189.cn.
authors: Xingyang
authors: X
authors: Liu
authors: Department of Gynecology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. youzeshan888@21cn.com.
authors: Zeshan
authors: Z
authors: You
authors: Department of Gynecology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. pgf_yang@126.com.
authors: Guofen
authors: G
authors: Yang
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D051152:Clusterin / Q000037:antagonists & inhibitors / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D016193:G1 Phase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D051152:Clusterin; D034741:RNA, Small Interfering