title: Exploring Genomic, Geographic and Virulence Interactions among Epidemic and Non-Epidemic St. Louis Encephalitis Virus (Flavivirus) Strains.
abstract: St. Louis encephalitis virus (SLEV) is a re-emerging arbovirus in South America. In 2005, an encephalitis outbreak caused by SLEV was reported in Argentina. The reason for the outbreak remains unknown, but may have been related to virological factors, changes in vectors populations, avian amplifying hosts, and/or environmental conditions. The main goal of this study was to characterize the complete genome of epidemic and non-epidemic SLEV strains from Argentina. Seventeen amino acid changes were detected; ten were non-conservative and located in proteins E, NS1, NS3 and NS5. Phylogenetic analysis showed two major clades based on geography: the North America and northern Central America (NAnCA) clade and the South America and southern Central America (SAsCA) clade. Interestingly, the presence of SAsCA genotype V SLEV strains in the NAnCA clade was reported in California, Florida and Texas, overlapping with known bird migration flyways. This work represents the first step in understanding the molecular mechanisms underlying virulence and biological variation among SLEV strains.
authors: Laboratorio de Arbovirus, Instituto de Virología ''Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Instituto de Investigaciones Biológicas y Tecnológicas, CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors: Luis A
authors: LA
authors: Diaz
authors: Área de Virosis Emergentes y Zoonóticas, Laboratorio de Ingeniería Genética y Biología Celular y Molecular, Instituto de Microbiología Básica y Aplicada, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
authors: Sandra E
authors: SE
authors: Goñi
authors: Laboratorio de Bioinformática Estructural, Fundación Instituto Leloir, CABA, Buenos Aires, Argentina.
authors: Javier A
authors: JA
authors: Iserte
authors: Laboratorio de Arbovirus, Instituto de Virología ''Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors: Agustín I
authors: AI
authors: Quaglia
authors: Centers for Diseases Control and Prevention. Fort Collins, Colorado, United States of America.
authors: Amber
authors: A
authors: Singh
authors: Novel and Dangerous Pathogens Training, Public Health England, Porton Down, United Kingdom.
authors: Christopher H
authors: CH
authors: Logue
authors: Centers for Diseases Control and Prevention. Fort Collins, Colorado, United States of America.
authors: Ann M
authors: AM
authors: Powers
authors: Laboratorio de Arbovirus, Instituto de Virología ''Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors: Marta S
authors: MS
authors: Contigiani
mesh_terms: D000818:Animals; D001118:Argentina; D002522:Chlorocebus aethiops; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000235:genetics*; D004665:Encephalitis Virus, St. Louis / Q000235:genetics / Q000472:pathogenicity; D004674:Encephalitis, St. Louis / Q000453:epidemiology / Q000235:genetics*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D014481:United States / Q000453:epidemiology; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins