title: Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System.
abstract: To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.25 and 100 μg/mL for both methods. The clenbuterol limit of detection for the target site compensation method and solution competition method are estimated to be 6.7 and 4.5 μg/mL, respectively. The proposed methods were successfully applied to the detection of clenbuterol molecules and were found to have high specificity and high-throughput and were label free and operationally convenient.
authors: Functional Materials Research Laboratory, Tongji University, Shanghai, People's Republic of China.
authors: Yichuan
authors: Y
authors: Wu
authors: Functional Materials Research Laboratory, Tongji University, Shanghai, People's Republic of China.
authors: Manwen
authors: M
authors: Yao
authors: School of Science, Xi'an Jiaotong University, Xi'an, People's Republic of China. xiangyifang@sina.com.
authors: Xiangyi
authors: X
authors: Fang
authors: Department of Clinical Laboratory, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors: Yucong
authors: Y
authors: Yang
authors: Department of Clinical Laboratory, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors: Xiaoli
authors: X
authors: Cheng
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002417:Cattle; D002976:Clenbuterol / Q000032:analysis*; D051379:Mice; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000906:Antibodies; D012710:Serum Albumin, Bovine; D002976:Clenbuterol
keywords: Food safety; Low-molecular-weight hapten detection; Multichannel measurement; Surface plasmon resonance biosensor