title: Isolation of dengue serotype 3 virus from the cerebrospinal fluid of an encephalitis patient in Hai Phong, Vietnam in 2013.
abstract: Dengue encephalitis (DE) is characterized as unusual presentation of dengue infection. Despite the reports that DE accounts for only 1-5% of dengue cases, this disease tends to be increasingly reported to threaten global human health throughout dengue endemic areas particularly in Southeast Asia. The molecular information of clinically characterized, neurotropic dengue virus (DENV) in human beings is extremely scarce despite it playing an important role in deciphering the pathogenesis of dengue-related neurological cases. Here we report a case of DE caused by DENV3 genotype III in a male patient with atypical symptoms of DENV infection in Hai Phong, Vietnam in 2013. The virus isolated from the cerebrospinal fluid of this case-patient was closely related to DENV3 genotype III strains isolated from serum of two other patients, who manifested classical dengue in the same year and residing in the same area as the case-patient. It is noteworthy to mention that in 2013, DENV3 genotype III was detected for the first time in Vietnam.
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; Graduate school of Biochemical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Minh Huong
authors: MH
authors: Phu Ly
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors: Yuki
authors: Y
authors: Takamatsu
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors: Takeshi
authors: T
authors: Nabeshima
authors: NIHE-Nagasaki Friendship Laboratory, Nagasaki University, Hanoi, Viet Nam.
authors: Linh Ly
authors: LL
authors: Pham Hoai
authors: NIHE-Nagasaki Friendship Laboratory, Nagasaki University, Hanoi, Viet Nam.
authors: Hang
authors: H
authors: Pham Thi
authors: NIHE-Nagasaki Friendship Laboratory, Nagasaki University, Hanoi, Viet Nam.
authors: Dinh
authors: D
authors: Dang Thi
authors: Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
authors: Ngoc Linh
authors: NL
authors: Nguyen
authors: Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
authors: Thu Thuy
authors: TT
authors: Nguyen Thi
authors: Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
authors: Quynh Mai
authors: QM
authors: Le Thi
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors: Corazon C
authors: CC
authors: Buerano
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors: Kouichi
authors: K
authors: Morita
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; Center of International Collaborative Research, Nagasaki University, Nagasaki, Japan. Electronic address: rainbow@nagasaki-u.ac.jp.
authors: Futoshi
authors: F
authors: Hasebe
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002555:Cerebrospinal Fluid / Q000821:virology*; D003715:Dengue / Q000175:diagnosis* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D018792:Encephalitis, Viral / Q000175:diagnosis* / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D065288:Serogroup; D014744:Vietnam
chemical_list: D000914:Antibodies, Viral
keywords: Atypical DENV infection; Case report; Dengue encephalitis; Dengue serotype 3 genotype III; Phylogenetic analysis