title: Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25.
abstract: BACKGROUND
  Molecularly imprinted polymer (MIP) technique is a powerful mean to produce tailor made synthetic recognition sites. Here precipitation polymerization was exploited to produce a library of MIP nanoparticles (NPs) targeting the N terminus of the hormone Hepcidin-25, whose serum levels correlate with iron dis-metabolisms and doping. Biotinylated MIP NPs were immobilized to NeutrAvidin™ SPR sensor chip. The response of the MIP NP sensor to Hepcidin-25 was studied.
  
  
  RESULTS
  Morphological analysis showed MIP NPs of 20-50 nm; MIP NP exhibited high affinity and selectivity for the target analyte: low nanomolar Kds for the interaction NP/Hepcidin-25, but none for the NP/non regulative Hepcidin-20. The MIP NP were integrated as recognition element in SPR allowing the detection of Hepcidin-25 in 3 min. Linearity was observed with the logarithm of Hepcidin-25 concentration in the range 7.2-720 pM. LOD was 5 pM. The response for Hepcidin-20 was limited. Hepcidin-25 determination in real serum samples spiked with known analyte concentrations was also attempted.
  
  
  CONCLUSIONS
  The integration of MIP NP to SPR allowed the determination of Hepcidin-25 at picomolar concentrations in short times outperforming the actual state of art. Optimization is still needed for real sample measurements in view of future clinical applications.
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. lucia.cenci@univr.it.
authors: Lucia
authors: L
authors: Cenci
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. erika.andreetto@univr.it.
authors: Erika
authors: E
authors: Andreetto
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. ambra.vestri@univr.it.
authors: Ambra
authors: A
authors: Vestri
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. michele.bovi@univr.it.
authors: Michele
authors: M
authors: Bovi
authors: Center for Materials and Microsystems CMM-MNF, FBK Fondazione Bruno Kessler, Via Sommarive 18, 38123, Povo-Trento, Italy. barozzi@fbk.eu.
authors: Mario
authors: M
authors: Barozzi
authors: Center for Materials and Microsystems CMM-MNF, FBK Fondazione Bruno Kessler, Via Sommarive 18, 38123, Povo-Trento, Italy. iacob@fbk.eu.
authors: Erica
authors: E
authors: Iacob
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. mirko.busato@univr.it.
authors: Mirko
authors: M
authors: Busato
authors: Department of Medicine, University of Verona, Section of Internal Medicine B, 37134, Verona, Italy. annalisa.castagna@univr.it.
authors: Annalisa
authors: A
authors: Castagna
authors: Department of Medicine, University of Verona, Section of Internal Medicine B, 37134, Verona, Italy. domenico.girelli@univr.it.
authors: Domenico
authors: D
authors: Girelli
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy. alessandramaria.bossi@univr.it.
authors: Alessandra Maria
authors: AM
authors: Bossi
mesh_terms: D064451:Hepcidins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D057230:Limit of Detection; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D064451:Hepcidins; C545475:hepcidin 25, human; D007501:Iron