title: Abnormal Localization of STK17A in Bile Canaliculi in Liver Allografts: An Early Sign of Chronic Rejection.
abstract: The biological significance of STK17A, a serine/threonine kinase, in the liver is not known. We analyzed STK17A expression in HepG2 cells and human liver tissue. Accordingly, we investigated whether STK17A could help in identifying earlier changes during the evolution of chronic rejection (CR) after liver transplantation. RT-PCR and immunofluorescence were used to analyze STK17A expression in HepG2 cells. Antibody microarray was performed using human liver samples from CR and healthy donors. Immunohistochemistry was used to verify the clinical utility of STK17A on sequential biopsies for the subsequent development of CR. A novel short isoform of STK17A was found in HepG2 cells. STK17A was localized in the nuclei and bile canaliculi in HepG2 cells and human livers. Microarray of STK17A revealed its decrease in failed liver allografts by CR. During the evolution of CR, the staining pattern of bile canalicular STK17A gradually changed from diffuse linear to focal intermittent. The focal intermittent staining pattern was observed before the definite diagnosis of CR. In conclusion, the present study was the first to find localization of STK17A in normal bile canaliculi. Abnormal expression and localization of STK17A were associated with CR of liver allografts since the early stage of the rejection process.
authors: Department of Forensic Medicine, Graduate school of Medicine, Kyoto University, Kyoto, Japan.
authors: Munetaka
authors: M
authors: Ozeki
authors: Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan.
authors: Adeeb
authors: A
authors: Salah
authors: Experimental and Clinical Research Center, Diabetes and Research Laboratory, Kocaeli University, Izmit, Turkey.
authors: Wulamujiang
authors: W
authors: Aini
authors: Department of Forensic Medicine, Graduate school of Medicine, Kyoto University, Kyoto, Japan.
authors: Keiji
authors: K
authors: Tamaki
authors: Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan.
authors: Hironori
authors: H
authors: Haga
authors: Center for Innovation in Immunoregulative Technology and Therapeutics, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors: Aya
authors: A
authors: Miyagawa-Hayashino
mesh_terms: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D064591:Allografts / Q000378:metabolism*; D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001648:Bile Canaliculi / Q000378:metabolism*; D001706:Biopsy; D003710:Demography; D005260:Female; D006084:Graft Rejection / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D053552:Keratin-7 / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D014019:Tissue Donors
chemical_list: C093491:ABCB11 protein, human; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; D051017:Apoptosis Regulatory Proteins; D053552:Keratin-7; D020033:Protein Isoforms; D017346:Protein Serine-Threonine Kinases; C115488:STK17A protein, human