title: Feasibility of bioethanol production from tubers of Dioscorea sansibarensis and Pyrenacantha kaurabassana.
abstract: Inedible tubers from Dioscorea sansibarensis (DS) and Pyrenacantha kaurabassana (PK) were found to be suitable feedstock for bioethanol production. Important composition parameters for bioethanol production for DS and PK are dry matter (% fresh tubers) ca. 20 and 6, total carbohydrates % dry weight base (db) ca. 68 and 47 and total protein (% db) ca. 16 and 10, respectively. DS and PK were found to contain inulin and galactomannan as principal polysaccharides (% of total carbohydrate) ca. 90 and 70, respectively. Diluted acid hydrolysis yielded ca. 100% of total reducing sugars. Ethanol yield ca. 56 and 35g/L was obtained at high efficiency through batch fermentation of acid hydrolysate (25% w/v) of DS and PK, respectively. A simple technique of recording and monitoring ethanol through CO2 generated during fermentation correlated strongly with HPLC measurement R(2)=0.99. Thus, tubers from these plants are potential feedstocks for bioethanol production with no competing uses.
authors: Tanzania Industrial Research and Development Organization, Kimweri Avenue, Msasani, TIRDO Complex, P.O. Box 23235, Dar es salaam, Tanzania; Department of Biochemistry and Nutrition, CSIR-Central Food Technology Research Institute, Mysore 570020, India. Electronic address: moshiap2000@gmail.com.
authors: Anselm P
authors: AP
authors: Moshi
authors: Tanzania Industrial Research and Development Organization, Kimweri Avenue, Msasani, TIRDO Complex, P.O. Box 23235, Dar es salaam, Tanzania; Department of Molecular Biology and Biotechnology, University of Dar es Salaam, Uvumbuzi Road, Box 35179, Dar es salaam, Tanzania.
authors: Jane P
authors: JP
authors: Nyandele
authors: Tanzania Industrial Research and Development Organization, Kimweri Avenue, Msasani, TIRDO Complex, P.O. Box 23235, Dar es salaam, Tanzania.
authors: Humphrey P
authors: HP
authors: Ndossi
authors: Department of Molecular Biology and Biotechnology, University of Dar es Salaam, Uvumbuzi Road, Box 35179, Dar es salaam, Tanzania.
authors: Sosovele M
authors: SM
authors: Eva
authors: Department of Molecular Biology and Biotechnology, University of Dar es Salaam, Uvumbuzi Road, Box 35179, Dar es salaam, Tanzania.
authors: Ken M
authors: KM
authors: Hosea
mesh_terms: D056804:Biofuels; D027742:Dioscorea / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism; D064028:Tracheophyta / Q000737:chemistry / Q000378:metabolism
chemical_list: D056804:Biofuels; D000431:Ethanol
keywords: Bioethanol; Carbon dioxide measurement; Dioscorea sansibarensis; Feedstock composition; Pyrenacantha kaurabassana