title: Investigating the Role of JAK/STAT Pathway on Dasatinib-Induced Apoptosis for CML Cell Model K562.
abstract: We aimed to evaluate the cytotoxic and apoptotic effects of dasatinib (BMS-354825) on K562 chronic myeloid leukemia (CML) cells and to examine the roles of STAT genes on dasatinib-induced apoptosis. The results showed that dasatinib decreased proliferation and induced apoptosis in K562 cells in a dose- and time-dependent manner. mRNA and protein levels of STAT5A and STAT5B genes were significantly reduced in dasatinib-treated K562 cells. These data indicated that STAT inhibition by dasatinib might be therapeutic in JAK/STAT pathway-associated malignancies after confirmation with clinical studies.
authors: Department of Internal Medicine, Ege University Medical Faculty, İzmir, Turkey. Electronic address: dr_ceydat@yahoo.com.
authors: Ceyda Tunakan
authors: CT
authors: Dalgıç
authors: Department of Medical Biology, Ege University Medical Faculty, İzmir, Turkey.
authors: Burçin Tezcanlı
authors: BT
authors: Kaymaz
authors: Department of Hematology, Ege University Medical Faculty, İzmir, Turkey.
authors: Melda Cömert
authors: MC
authors: Özkan
authors: Department of Medical Biology, Ege University Medical Faculty, İzmir, Turkey.
authors: Ayşegül
authors: A
authors: Dalmızrak
authors: Department of Hematology, Ege University Medical Faculty, İzmir, Turkey.
authors: Fahri
authors: F
authors: Şahin
authors: Department of Hematology, Ege University Medical Faculty, İzmir, Turkey.
authors: Güray
authors: G
authors: Saydam
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000069439:Dasatinib / Q000627:therapeutic use*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy*; D050799:STAT5 Transcription Factor; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins
chemical_list: D050799:STAT5 Transcription Factor; C494092:STAT5A protein, human; D025521:Tumor Suppressor Proteins; D000069439:Dasatinib
keywords: Apoptosis; Chronic myeloid leukemia; Dasatinib; JAK/STAT pathway; STAT5