title: Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review.
abstract: Membrane-based lateral flow immunochromatographic strip (LFICS) is widely used in various fields because of its simplicity, rapidity (detection within 10min), and low cost. However, early designs of membrane-based LFICS for preliminary screening only provide qualitative ("yes/no" signal) or semi-quantitative results without quantitative information. These designs often suffer from low-signal intensity and poor sensitivity and are only capable of single analyte detection, not simultaneous multiple detections. The performance of existing techniques used for detection using LFICS has been considerably improved by incorporating different kinds of nanoparticles (NPs) as reporters. NPs can serve as alternative labels and improve analytical sensitivity or limit of detection of LFICS because of their unique properties, such as optical absorption, fluorescence spectra, and magnetic properties. The controlled manipulation of NPs allows simultaneous or multiple detections by using membrane-based LFICS. In this review, we discuss how colored (e.g., colloidal gold, carbon, and colloidal selenium NPs), luminescent (e.g., quantum dots, up-converting phosphor NPs, and dye-doped NPs), and magnetic NPs are integrated into membrane-based LFICS for the detection of target analytes. Gold NPs are also featured because of their wide applications. Different types and unique properties of NPs are briefly explained. This review focuses on examples of NP-based LFICS to illustrate novel concepts in various devices with potential applications as screening tools. This review also highlights the superiority of NP-based approaches over existing conventional strategies for clinical analysis, food safety, and environmental monitoring. This paper is concluded by a short section on future research trends regarding NP-based LFICS.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China.
authors: Xiaolin
authors: X
authors: Huang
authors: Zystein, LLC., Fayetteville, AR 72704, USA.
authors: Zoraida P
authors: ZP
authors: Aguilar
authors: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, PR China. Electronic address: kidyxu@163.com.
authors: Hengyi
authors: H
authors: Xu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, PR China.
authors: Weihua
authors: W
authors: Lai
authors: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China. Electronic address: yhxiongchen@163.com.
authors: Yonghua
authors: Y
authors: Xiong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003102:Colloids / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry
chemical_list: D003102:Colloids; D002244:Carbon; D006046:Gold; D012643:Selenium
keywords: Colored nanoparticles; Detection; Lateral flow immunochromatographic strip; Luminescent nanoparticles; Magnetic nanoparticles