title: Association of vdr, cyp27b1, cyp24a1 and mthfr gene polymorphisms with oral lichen planus risk.
abstract: OBJECTIVE
  The current study investigated the association between VDR EcoRV (rs4516035), FokI (rs2228570), ApaI (rs7975232) and TaqI (rs731236), CYP27B1 (rs4646536), CYP24A1 (rs2296241), and MTHFR (rs1801133) gene polymorphisms and risk of oral lichen planus (OLP) occurrence.
  
  
  METHODS
  The study group consisted of 65 oral lichen planus patients and 100 healthy blood donors in the control group. Single nucleotide polymorphisms were genotyped by real time PCR or PCR-restriction fragment length polymorphism (RFLP) method.
  
  
  RESULTS
  Heterozygous as well as mutated genotype of vitamin D receptor (VDR) FokI (rs2228570) polymorphism was associated with increased oral lichen planus risk in comparison with wild type genotype (odds ratio (OR) = 3.877, p = 0.017, OR = 38.153, p = 0.001, respectively). A significantly decreased OLP risk was observed for heterozygous genotype of rs2296241 polymorphism in CYP24A1 gene compared with the wild type form (OR = 0.314, p = 0.012). VDR gene polymorphisms ApaI and TaqI were in linkage disequilibrium (D' = 0.71, r(2) = 0.22). Identified haplotype AT was associated with decreased OLP risk (OR = 0.592, p = 0.047).
  
  
  CONCLUSIONS
  Our results highlight the possible important role of VDR FokI (rs2228570) and CYP24A1 rs2296241 gene polymorphisms for oral lichen planus susceptibility.
  
  
  CONCLUSIONS
  Identification of new molecular biomarkers could potentially contribute to determination of individuals with OLP predisposition.
authors: Department of Dentistry-Oral Surgery, Faculty of Medicine, University of East Sarajevo, Foca, Bosnia and Herzegovina.
authors: Bojan
authors: B
authors: Kujundzic
authors: Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia. katjaze@yahoo.com.|Faculty of Biology, University of Belgrade, Studentski Trg 3, Belgrade, 11000, Serbia. katjaze@yahoo.com.
authors: Katarina
authors: K
authors: Zeljic
authors: Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia.|Faculty of Medicine, Military Medical Academy, University of Defense, Belgrade, Serbia.
authors: Gordana
authors: G
authors: Supic
authors: School of Dental Medicine, Clinic of Periodontology and Oral Medicine, University of Belgrade, Belgrade, Serbia.
authors: Marko
authors: M
authors: Magic
authors: School of Dental Medicine, Clinic of Periodontology and Oral Medicine, University of Belgrade, Belgrade, Serbia.
authors: Dragan
authors: D
authors: Stanimirovic
authors: Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia.
authors: Vesna
authors: V
authors: Ilic
authors: Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia.
authors: Barbara
authors: B
authors: Jovanovic
authors: Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia.|Faculty of Medicine, Military Medical Academy, University of Defense, Belgrade, Serbia.
authors: Zvonko
authors: Z
authors: Magic
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol; D065668:Vitamin D3 24-Hydroxylase / Q000235:genetics
chemical_list: D018167:Receptors, Calcitriol; D065668:Vitamin D3 24-Hydroxylase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase
keywords: CYP24A1; CYP27B1; MTHFR; Oral lichen planus; Single nucleotide polymorphisms; VDR