title: Biomedical potential of actinobacterially synthesized selenium nanoparticles with special reference to anti-biofilm, anti-oxidant, wound healing, cytotoxic and anti-viral activities.
abstract: Currently, there is an ever-increasing need to develop environmentally benign processes in place of synthetic protocols. As a result, researchers in the field of nanoparticle synthesis are focusing their attention on microbes from rare biological ecosystems. One potential actinobacterium, Streptomyces minutiscleroticus M10A62 isolated from a magnesite mine had the ability to synthesize selenium nanoparticles (SeNPs), extracellularly. Actinobacteria mediated SeNP synthesis were characterized by UV-visible, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX) and high resolution transmission electron microscopy (HR-TEM) analysis. The UV-spectral analysis of SeNPs indicated the maximum absorption at 510nm, FT-IR spectral analysis confirms the presence of capping protein, peptide, amine and amide groups. The selenium signals confirm the presence of SeNPs. All the diffraction peaks in the XRD pattern and HR-TEM confirm the size of SeNPs in the range of 10-250nm. Further, the anti-biofilm and antioxidant activity of the SeNPs increased proportionally with rise in concentration, and the test strains reduced to 75% at concentration of 3.2μg. Selenium showed significant anti-proliferative activity against HeLa and HepG2 cell lines. The wound healing activity of SeNPs reveals that 5% selenium oinment heals the excision wound of Wistar rats up to 85% within 18 days compared to the standard ointment. The biosynthesized SeNPs exhibited good antiviral activity against Dengue virus. The present study concludes that extremophilic actinobacterial strain was a novel source for SeNPs with versatile biomedical applications and larger studies are needed to quantify these observed effects of SeNPs.
authors: Actinobacterial Research Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India; Center for Nanoscience and Nanotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India.
authors: Suseenthar
authors: S
authors: Ramya
authors: Actinobacterial Research Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India.
authors: Thangavel
authors: T
authors: Shanmugasundaram
authors: Actinobacterial Research Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India; Center for Nanoscience and Nanotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India. Electronic address: actinobalaguru@gmail.com.
authors: Ramasamy
authors: R
authors: Balagurunathan
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003716:Dengue Virus / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000187:drug effects / Q000648:ultrastructure; D014945:Wound Healing / Q000187:drug effects*; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D000998:Antiviral Agents; D012643:Selenium
keywords: Anti-biofilm; Anti-proliferative; Selenium nanoparticle; Streptomyces minutiscleroticus; Wound healing