title: Biophysical characterization of the interaction between FAAP20-UBZ4 domain and Rev1-BRCT domain.
abstract: FAAP20 (Fanconi anemia-associated protein 20) is a subunit of the Fanconi anemia (FA) core complex that repairs interstrand cross-links. To understand the molecular basis for the FA core complex-mediated recruitment of Rev1 to the DNA lesion, we characterized the interactions among FAAP20-UBZ4, Rev1-BRCT, and ubiquitin using NMR. We found that FAAP20-UBZ4 binds not only ubiquitin but also Rev1-BRCT. Mapping the protein-protein interactions showed that FAAP20-UBZ4 has distinct binding surfaces for ubiquitin and Rev1-BRCT. In addition, the chemical exchange patterns indicated that the interaction between FAAP20-UBZ4 and ubiquitin might enhance the binding affinity between FAAP20-UBZ4 and Rev1-BRCT. These results provide new insight into the Rev1 recognition mechanism by FAAP20.
authors: Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Kyungeun
authors: K
authors: Lim
authors: Structural biology & Nanopore laboratory, Functional Genomics Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
authors: Mi-Kyung
authors: MK
authors: Lee
authors: Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Phuong T M
authors: PT
authors: Duong
authors: Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Dinan
authors: D
authors: Liu
authors: Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Sieun
authors: S
authors: Sung
authors: Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: byongseok.choi@kaist.ac.kr.
authors: Byong-Seok
authors: BS
authors: Choi
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D055592:Biophysical Phenomena; D051856:Fanconi Anemia Complementation Group Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013499:Surface Properties; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism; D000099270:Y-Family DNA Polymerases
chemical_list: D051856:Fanconi Anemia Complementation Group Proteins; D009687:Nuclear Proteins; D009713:Nucleotidyltransferases; D025801:Ubiquitin; C570807:FAAP20 protein, human; C429004:REV1 protein, human; D000099270:Y-Family DNA Polymerases
keywords: Fanconi anemia pathway; Fanconi anemia-associated protein 20; Interstrand cross-link; Nuclear magnetic resonance; Rev1; Ubiquitin