title: Sol-gel synthesis of quaternary (P2O5)55-(CaO)25-(Na2O)(20-x)-(TiO2) x bioresorbable glasses for bone tissue engineering applications (x = 0, 5, 10, or 15).
abstract: In the present study, we report a new and facile sol-gel synthesis of phosphate-based glasses with the general formula of (P2O5)55-(CaO)25-(Na2O)(20-x)-(TiO2) x , where x = 0, 5, 10 or 15, for bone tissue engineering applications. The sol-gel synthesis method allows greater control over glass morphology at relatively low processing temperature (200 °C) in comparison with phosphate-based melt-derived glasses (~1000 °C). The glasses were analyzed using several characterization techniques, including x-ray diffraction (XRD), (31)P magic angle spinning nuclear magnetic resonance ((31)P MAS-NMR), Fourier transform infrared (FTIR) spectroscopy and energy-dispersive x-ray (EDX) spectroscopy, which confirmed the amorphous and glassy nature of the prepared samples. Degradation was assessed by measuring the ion release and pH change of the storage medium. Cytocompatibility was also confirmed by culturing osteoblast-like osteosarcoma cell line MG-63 on the glass microparticles over a seven-day period. Cell attachment to the particles was imaged using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results revealed the potential of phosphate-based sol-gel derived glasses containing 5 or 10 mol% TiO2, with high surface area, ideal dissolution rate for cell attachment and easily metabolized dissolution products, for bone tissue engineering applications.
authors: Division of Biomaterials & Tissue Engineering, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK. Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK.
authors: Farzad
authors: F
authors: Foroutan
authors: Nick J
authors: NJ
authors: Walters
authors: Gareth J
authors: GJ
authors: Owens
authors: Nicola J
authors: NJ
authors: Mordan
authors: Hae-Won
authors: HW
authors: Kim
authors: Nora H
authors: NH
authors: de Leeuw
authors: Jonathan C
authors: JC
authors: Knowles
mesh_terms: D020341:Absorbable Implants; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D044367:Phase Transition
chemical_list: D018786:Bone Substitutes