title: [Preparation and Assessment of IL1RAP Monoclonal Antibody].
abstract: OBJECTIVE
  To prepare and identify human monoclonal antibody against IL-1 receptor accessory protein (IL1RAP), which is a new identified surface marker for leukemia stem cells (LSC).
  
  
  METHODS
  BALB/c mice were inoculated intraperitoneally with hybridoma cells (3H6E10, 10D8A7) and their ascites were collected. The monoclonal antibody against hu-IL1RAP specifically was purified from ascites, the nondenaturing-PAGE, ELISA and Western blot were used to detect the purity, titer and sensitivity of antibody.
  
  
  RESULTS
  Two purified antibodies were obtained and named as 3H6E10 McAb and 10D8A7 McAb, whose purity was 95% and 94% respectively. The titer of two purified monoclonal antibodies was 1 : 81000 and specific conjugation of IL1RAP purified protein and endogenous protein from normal people and leukemia patients with purified antibodies were confirmed.
  
  
  CONCLUSIONS
  The purified monoclonal antibodies which can specifically bind to hu-IL1RAP are successfully prepared, thus providing novel way to effectively clear LSC in the future.
authors: Blood Diseases Institute, Key Labatory of Bone Marrow Stem Cells, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors: Ling-Ling
authors: LL
authors: Yin
authors: Blood Diseases Institute, Key Labatory of Bone Marrow Stem Cells, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors: Su-Hong
authors: SH
authors: Ruan
authors: Blood Diseases Institute, Key Labatory of Bone Marrow Stem Cells, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors: Yu
authors: Y
authors: Tian
authors: Blood Diseases Institute, Key Labatory of Bone Marrow Stem Cells, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.|Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail: limd@163.com.
authors: Kai Lin
authors: KL
authors: Xu
authors: Blood Diseases Institute, Key Labatory of Bone Marrow Stem Cells, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.|Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail: kainyzhao@163.com.
authors: Kai
authors: K
authors: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas; D053577:Interleukin-1 Receptor Accessory Protein; D007938:Leukemia; D051379:Mice; D008807:Mice, Inbred BALB C; D014411:Neoplastic Stem Cells
chemical_list: D000911:Antibodies, Monoclonal; C507765:IL1RAP protein, human; D053577:Interleukin-1 Receptor Accessory Protein