title: Detection of adulterated murine components in meat products by TaqMan© real-time PCR.
abstract: Using murine meat to substitute mutton has been identified as a new type of meat fraud in China, yet no detection method for murine species has been reported. Here, three kinds of rodent were used as target species to establish a murine-specific real-time PCR method of detection. The mitochondrial cytochrome b gene (cytb) of each target was sequenced and a TaqMan probe was designed based on the cytb. Simultaneously, an internal positive control (IPC) plasmid along with its respective probe were designed to monitor the PCR reaction. As a result, the duplex real-time PCR system was verified to be specific. The limit of detection (LOD) was lower than 1 pg of DNA per reaction and 0.1% murine contamination in meat mixtures. Standard curves were generated for a quantitative analysis. Thus, this study provided a new tool to control the quality of meat products for official and third-party laboratories.
authors: National Center of Supervision & Inspection on Processed Food and Food Additives Quality, Nanjing Institute of Product Quality Inspection, No. 3 Jialingjiang East Street, 210019 Nanjing, China.
authors: Xin
authors: X
authors: Fang
authors: National Center of Supervision & Inspection on Processed Food and Food Additives Quality, Nanjing Institute of Product Quality Inspection, No. 3 Jialingjiang East Street, 210019 Nanjing, China; National Key Laboratory of Bioelectronics, Southeast University, No. 2 Sipai Lou, 210096 Nanjing, China. Electronic address: cavy78@gmail.com.
authors: Chi
authors: C
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D045303:Cytochromes b / Q000235:genetics; D004247:DNA / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D063427:Food Quality; D005607:Fraud; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D051379:Mice; D010957:Plasmids; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA; D045303:Cytochromes b
keywords: Adulteration; Meat products; Murine meat; Real-time PCR