title: Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses.
abstract: Transcription activator-like effectors (TALEs) are a class of sequence-specific DNA-binding proteins that utilize a simple and predictable modality to recognize target DNA. This unique characteristic allows for the rapid assembly of artificial TALEs, with high DNA binding specificity, to any target DNA sequences for the creation of customizable sequence-specific nucleases used in genome engineering. Here, we report the use of an artificial TALE protein as a convenient platform for designing broad-spectrum resistance to begomoviruses, one of the most destructive plant virus groups, which cause tremendous losses worldwide. We showed that artificial TALEs, which were assembled based on conserved sequence motifs within begomovirus genomes, could confer partial resistance in transgenic Nicotiana benthamiana to all three begomoviruses tested. Furthermore, the resistance was maintained even in the presence of their betasatellite. These results shed new light on the development of broad-spectrum resistance against DNA viruses, such as begomoviruses.
authors: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China. xfcheng@hznu.edu.cn.
authors: Xiaofei
authors: X
authors: Cheng
authors: Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China. elva1988@163.com.
authors: Fangfang
authors: F
authors: Li
authors: College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, China. cjy19920514@hotmail.com.
authors: Jianyu
authors: J
authors: Cai
authors: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China. idave.01@163.com.
authors: Wei
authors: W
authors: Chen
authors: Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China. zhaonanzilv@163.com.
authors: Nan
authors: N
authors: Zhao
authors: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China. sunyuq1109@hotmail.com.
authors: Yuqiang
authors: Y
authors: Sun
authors: Key Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou, Institute of Tobacco Science, Guiyang 550083, Guizhou, China. yshguo@126.com.
authors: Yushuang
authors: Y
authors: Guo
authors: Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China. xiulingyang@zju.edu.cn.
authors: Xiuling
authors: X
authors: Yang
authors: College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, China. wxy5551@126.com.
authors: Xiaoyun
authors: X
authors: Wu
mesh_terms: D053839:Begomovirus / Q000276:immunology*; D060467:Disease Resistance; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology*; D030821:Plants, Genetically Modified; D015202:Protein Engineering; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors
keywords: Antivirus; Begomovirus; Broad-spectrum resistance; DNA-binding protein; Transcription activator–like effectors