title: [In vivo Cell CFSE Fluorescence Negative Staining for Detection of Super Paramagnetic Iron Oxide Nanoparticles Phagocytosed by Mouse Mononuclear Macrophage Leukemia Cells-RAW264.7].
abstract: OBJECTIVE
  To explore the feasibility and fluorescence characteristics of CFSE negative staining for in vivo cell imaging of super paramagnetic iron oxide nanoparticles (SPIO) phagocytosed by mouse mononuclear macrophage leukemia cells-RAW264.7.
  
  
  METHODS
  After labeled with SPIO, the RAW264.7 macrophages were stained with Prussian blue stain and CFSE fluorescence negative stain step by step. Furthermore, trypan blue staining was used to evaluate cell viability of cells which stained with CFSE. At last, laser scanning confocal microscope was used to measure SPIO in cells through CFSE fluorescence negative stain method.
  
  
  RESULTS
  SPIO within RAW264.7 macrophages showed blue in Prussian's blue staining, while showed negative area in CFSE negative staining. Good consistencies between Prussian's blue staining and CFSE negative staining were observed. In addition, RAW264.7 macrophages showed high viability after SPIO/CFSE dual-labeled method, proved by typan stain.
  
  
  CONCLUSIONS
  The CFSE fluorescence negative staining may be used for detecting SPIO that phagocytosed by RAW264.7 macrophages and it is showed good consistency that confirmed one another when compared to classic Prussian' blue staining.
authors: Department of Hematology (Key Discipline of Medicine, Jiangsu Province), Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China.|Department of Internal Medicine, Nantong Cancer Hospital Affiliated to Nantong University, Nantong 226361, Jiangsu Province, China.
authors: Xiang-Feng
authors: XF
authors: He
authors: Central Laboratory, Nantong Cancer Hospital Affiliated to Nantong University, Nantong 226361, Jiangsu Province, China.
authors: Li-Ping
authors: LP
authors: Yang
authors: Department of Hematology (Key Discipline of Medicine, Jiangsu Province), Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China. E-mail: bachen@seu.edu.cn.
authors: Bao-An
authors: BA
authors: Chen
authors: Department of Internal Medicine, Nantong Cancer Hospital Affiliated to Nantong University, Nantong 226361, Jiangsu Province, China. E-mail: ywk_wjh@163.com.
authors: Jian-Hong
authors: JH
authors: Wang
authors: Department of Intervention, The Second Hospital Affiliated to Nancang University, Nancang 330006, Jiangxi Province China.
authors: Song
authors: S
authors: Wen
authors: Department of General Surgery, The Second Affiliated Hospital, Nantong University, Nantong 226001, Jiangsu Province, China.
authors: Wen
authors: W
authors: Shi
authors: Department of Internal Medicine, Nantong Cancer Hospital Affiliated to Nantong University, Nantong 226361, Jiangsu Province, China.
authors: Wei-Wei
authors: WW
authors: Xu
authors: Department of Internal Medicine, Nantong Cancer Hospital Affiliated to Nantong University, Nantong 226361, Jiangsu Province, China.
authors: Guo-Jie
authors: GJ
authors: Ling
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D003287:Contrast Media; D005290:Ferric Compounds; D005295:Ferrocyanides; D005452:Fluoresceins; D005453:Fluorescence; D007938:Leukemia; D008264:Macrophages; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D051379:Mice; D016624:Negative Staining; D010587:Phagocytosis; D013388:Succinimides
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D003287:Contrast Media; D005290:Ferric Compounds; D005295:Ferrocyanides; D005452:Fluoresceins; D058185:Magnetite Nanoparticles; D013388:Succinimides; C000499:ferric oxide; C000170:ferric ferrocyanide