title: A reverse phased high-pressure liquid chromatographic method for the estimation of a poisonous matter in Strychnos nux-vomica.
abstract: Seeds of Strychnos nux-vomica were subjected to preliminary phytochemical tests and its presence was confirmed by thin layer chromatography (TLC) method. The TLC profile of the methanolic extract of seeds of S. nux-vomica was developed using the solvent system toluene:chloroform:methanol in the ratio 8:2:1. The plate was observed in visible light after spraying with Dragendorff's reagent (specific method). High-performance liquid chromatography (HPLC) profile of the methanolic extracts of S. nux-vomica was developed, and the amount of strychnine seems to be 0.36% (w/w) in the seeds. The TLC and HPLC profiles developed are very valuable for the identification of the original drug from their adulterants. The TLC profile identifies the presence of strychnine in the plant material. The quantification method for the strychnine in the seeds can be used for the quality standardization of the raw drug because the strychnine is reported to have some toxicity.
authors: Department of Forensic Medicine and Toxicology, Yenepoya Medical College, Mangalore, India.
authors: Achu
authors: A
authors: Hashim
authors: Department of Cleft and Craniofacial Surgery, Yenepoya University, Mangalore, India.
authors: Rafique
authors: R
authors: Mohammed
authors: Department of Cleft and Craniofacial Surgery, Yenepoya University, Mangalore, India.
authors: Dilshad
authors: D
authors: Umar
authors: Department of Forensic Medicine and Toxicology, Yenepoya Medical College, Mangalore, India.
authors: Vaswani R
authors: VR
authors: Veena
authors: Department of Cleft and Craniofacial Surgery, Yenepoya University, Mangalore, India.
authors: Basheer
authors: B
authors: Bahija
authors: Department of Cleft and Craniofacial Surgery, Yenepoya University, Mangalore, India.
authors: Baroudi
authors: B
authors: Kusai
keywords: High-performance liquid chromatography; Strychnos nux-vomica seeds; strychnine; thin layer chromatography