title: Studying the relationship between cell cycle and Alzheimer's disease by gold nanoparticle probes.
abstract: In this study, a simple gold nanoparticle (GNP)-based colorimetric assay has been developed for studying the relationship between cell cycle and β-amyloid peptide (Aβ, the biomarker of Alzheimer's disease [AD]) expression level. It was found that Aβ expression of neuronal cells (e.g., SHG-44 cell line) is strongly dependent on cell cycle phases; that is, the Aβ expression level was highest when cells were arrested in the G1/S phase by thymidine and was lowest when they were arrested in the G2/M phase by nocodazole. This finding may improve the understanding of AD pathology and provide a new tool for anti-dementia drug development.
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Chengke
authors: C
authors: Wang
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: wangzx@ciac.ac.cn.
authors: Zhenxin
authors: Z
authors: Wang
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism; D000963:Antimetabolites / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D019921:Biotinylation; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D016193:G1 Phase / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D006046:Gold / Q000032:analysis / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D050257:Tubulin Modulators / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; D000963:Antimetabolites; D015415:Biomarkers; D055767:Immobilized Proteins; D015335:Molecular Probes; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D050257:Tubulin Modulators; C433241:amyloid beta-protein (1-16); D006046:Gold; D019809:Streptavidin
keywords: Alzheimer's disease; Cell cycle; Gold nanoparticle