title: Genotyping of 75 SNPs using arrays for individual identification in five population groups.
abstract: Single nucleotide polymorphism (SNP) typing offers promise to forensic genetics. Various strategies and panels for analyzing SNP markers for individual identification have been published. However, the best panels with fewer identity SNPs for all major population groups are still under discussion. This study aimed to find more autosomal SNPs with high heterozygosity for individual identification among Asian populations. Ninety-six autosomal SNPs of 502 DNA samples from unrelated individuals of five population groups (208 Taiwanese Han, 83 Filipinos, 62 Thais, 69 Indonesians, and 80 individuals with European, Near Eastern, or South Asian ancestry) were analyzed using arrays in an initial screening, and 75 SNPs (group A, 46 newly selected SNPs; groups B, 29 SNPs based on a previous SNP panel) were selected for further statistical analyses. Some SNPs with high heterozygosity from Asian populations were identified. The combined random match probability of the best 40 and 45 SNPs was between 3.16 × 10(-17) and 7.75 × 10(-17) and between 2.33 × 10(-19) and 7.00 × 10(-19), respectively, in all five populations. These loci offer comparable power to short tandem repeats (STRs) for routine forensic profiling. In this study, we demonstrated the population genetic characteristics and forensic parameters of 75 SNPs with high heterozygosity from five population groups. This SNPs panel can provide valuable genotypic information and can be helpful in forensic casework for individual identification among these populations.
authors: Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China.|Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan, Republic of China.|Department of Medical Genetics, National Taiwan University Hospital, Taipei, Taiwan, Republic of China.
authors: Hsiao-Lin
authors: HL
authors: Hwa
authors: Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan, Republic of China.
authors: Lawrence Shih Hsin
authors: LS
authors: Wu
authors: Institute of Forensic Medicine, Ministry of Justice, Taipei, Taiwan, Republic of China.
authors: Chun-Yen
authors: CY
authors: Lin
authors: Institute of Forensic Medicine, Ministry of Justice, Taipei, Taiwan, Republic of China.
authors: Tsun-Ying
authors: TY
authors: Huang
authors: Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China.
authors: Hsiang-I
authors: HI
authors: Yin
authors: Department of Medical Genetics, National Taiwan University Hospital, Taipei, Taiwan, Republic of China.
authors: Li-Hui
authors: LH
authors: Tseng
authors: Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China. jimlee@ntu.edu.tw.
authors: James Chun-I
authors: JC
authors: Lee
mesh_terms: D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population; D060005:Genotyping Techniques; D060148:HapMap Project; D006579:Heterozygote; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D044469:Racial Groups / Q000235:genetics*; D012189:Retrospective Studies
chemical_list: D004247:DNA
keywords: Array; Forensic genetics; Individual identification; Random match probability; Single nucleotide polymorphism