title: Architecture of the fungal nuclear pore inner ring complex.
abstract: The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary analyses of the ~425-kilodalton inner ring complex (IRC), which forms the central transport channel and diffusion barrier of the NPC, revealing its interaction network and equimolar stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96. The CNT•Nic96 structure reveals that Nic96 functions as an assembly sensor that recognizes the three-dimensional architecture of the CNT, thereby mediating the incorporation of a defined CNT state into the NPC. We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation.
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Tobias
authors: T
authors: Stuwe
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Christopher J
authors: CJ
authors: Bley
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Karsten
authors: K
authors: Thierbach
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Stefan
authors: S
authors: Petrovic
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Sandra
authors: S
authors: Schilbach
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Daniel J
authors: DJ
authors: Mayo
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Thibaud
authors: T
authors: Perriches
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Emily J
authors: EJ
authors: Rundlet
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Young E
authors: YE
authors: Jeon
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Leslie N
authors: LN
authors: Collins
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Ferdinand M
authors: FM
authors: Huber
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.
authors: Daniel H
authors: DH
authors: Lin
authors: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors: Marcin
authors: M
authors: Paduch
authors: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors: Akiko
authors: A
authors: Koide
authors: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors: Vincent
authors: V
authors: Lu
authors: Biochemistry Center of Heidelberg University, 69120 Heidelberg, Germany.
authors: Jessica
authors: J
authors: Fischer
authors: Biochemistry Center of Heidelberg University, 69120 Heidelberg, Germany.
authors: Ed
authors: E
authors: Hurt
authors: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors: Shohei
authors: S
authors: Koide
authors: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors: Anthony A
authors: AA
authors: Kossiakoff
authors: California Institute of Technology, Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA. hoelz@caltech.edu.
authors: André
authors: A
authors: Hoelz
mesh_terms: D000595:Amino Acid Sequence; D002597:Chaetomium / Q000378:metabolism / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000737:chemistry / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D022022:Nuclear Pore / Q000378:metabolism / Q000648:ultrastructure*; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000737:chemistry / Q000648:ultrastructure*; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D005656:Fungal Proteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins