title: Multifunctional Chitosan-45S5 Bioactive Glass-Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Microsphere Composite Membranes for Guided Tissue/Bone Regeneration.
abstract: Novel multifunctional chitosan-45S5 bioactive glass-poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microsphere (CS-BG-MS) composite membranes were developed with applicability in guided tissue/bone regeneration (GTR/GBR). The incorporation of 45S5 BG and PHBV MS into CS membranes not only provided the membranes with favorable surface roughness, hydrophilicity, and flexibility but also slowed down their degradation rate. Moreover, the CS membranes became bioactive after the incorporation of 45S5 BG and capable of releasing drugs of different physicochemical properties in a controlled and sustained manner with the addition of PHBV MS. Cell culture tests showed that osteoblast-like MG-63 human osteosarcoma cells had significantly higher adhesion, cell proliferation, and alkaline phosphatase (ALP) activity on CS-BG and CS-BG-MS membranes than on neat CS membranes. Therefore, the developed bioactive CS-BG-MS membranes with potential multidrug (e.g., antibacterial and antiosteoporosis drugs) delivery capability are promising candidate membranes for GTR/GBR applications.
authors: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg , Cauerstrasse 6, 91058 Erlangen, Germany.
authors: Wei
authors: W
authors: Li
authors: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg , Martensstrasse 7, 91058 Erlangen, Germany.
authors: Yaping
authors: Y
authors: Ding
authors: Institute of Advanced Materials for Nano-Bio Applications, School of Ophthalmology and Optometry, Wenzhou Medical University , 270 Xueyuan Xi Road, Wenzhou, Zhejiang 325027, China.
authors: Shanshan
authors: S
authors: Yu
authors: Institute of Advanced Materials for Nano-Bio Applications, School of Ophthalmology and Optometry, Wenzhou Medical University , 270 Xueyuan Xi Road, Wenzhou, Zhejiang 325027, China.
authors: Qingqing
authors: Q
authors: Yao
authors: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg , Cauerstrasse 6, 91058 Erlangen, Germany.
authors: Aldo R
authors: AR
authors: Boccaccini
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D065546:Drug Liberation; D005898:Glass; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D008863:Microspheres; D011091:Polyesters / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013752:Tetracycline / Q000494:pharmacology; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction; D000094122:Polyhydroxybutyrates
chemical_list: D000469:Alkaline Phosphatase; D048271:Chitosan; D008567:Membranes, Artificial; D011091:Polyesters; D013752:Tetracycline; D014867:Water; C425330:bioactive glass 45S5; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: 45S5 bioactive glass; PHBV microsphere; chitosan; drug delivery; guided bone regeneration; membrane