title: Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: A sensitive, confirmatory ultra-high performance liquid chromatography-tandem mass spectrometric method was developed and validated to detect 23 veterinary drugs and metabolites (nitroimidazoles, benzimidazoles, and chloramphenicol components) in bovine milk. Compounds of interest were sequentially extracted from milk with acetonitrile and basified acetonitrile using sodium chloride to induce liquid-liquid partition. The extract was purified on a mixed mode solid-phase extraction cartridge. Using rapid polarity switching in electrospray ionization, a single injection was capable of detecting both positively and negatively charged analytes in a 9 min chromatography run time. Recoveries based on matrix-matched calibrations and isotope labeled internal standards for milk ranged from 51.7% to 101.8%. The detection limits and quantitation limits of the analytical method were found to be within the range of 2-20 ng/kg and 5-50 ng/kg, respectively. The recommended method is simple, specific, and reliable for the routine monitoring of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk samples.
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors: Yuanyuan
authors: Y
authors: Wang
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, Ministry of Agriculture, Beijing 100193, China.
authors: Xiaowei
authors: X
authors: Li
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Shunyi Center for Animal Disease Control and Prevention, Beijing 101300, China.
authors: Zhiwen
authors: Z
authors: Zhang
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, Ministry of Agriculture, Beijing 100193, China.
authors: Shuangyang
authors: S
authors: Ding
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, Ministry of Agriculture, Beijing 100193, China.
authors: Haiyang
authors: H
authors: Jiang
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, Ministry of Agriculture, Beijing 100193, China.
authors: Jiancheng
authors: J
authors: Li
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, Ministry of Agriculture, Beijing 100193, China.
authors: Jianzhong
authors: J
authors: Shen
authors: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, China Agricultural University, Beijing 100193, China; Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, Ministry of Agriculture, Beijing 100193, China. Electronic address: xxia@cau.edu.cn.
authors: Xi
authors: X
authors: Xia
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000032:analysis; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D009593:Nitroimidazoles / Q000032:analysis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D001562:Benzimidazoles; D009593:Nitroimidazoles; D019155:Veterinary Drugs; D002701:Chloramphenicol
keywords: Benzimidazoles; Chloramphenicol components; Nitroimidazoles; Tandem mass spectrometry; Ultra-high performance liquid chromatography