title: Cytotoxicity and genotoxicity of natural resin-based experimental endodontic sealers.
abstract: OBJECTIVE
  The development of endodontic sealers based on natural resins seems to be promising, given their improved biological properties. This study evaluated the cytotoxic and genotoxic effects of two experimental root canal sealers, based on extracts from Copaifera multijuga and Ricinus communis (castor oil polymer), comparing them to synthetic resin-based sealers: a single methacrylate-based, a multi-methacrylate-based, and an epoxy resin-based sealers.
  
  
  METHODS
  Sealers were prepared, set, and exposed to cell culture medium for 24 h at 37 °C with CO2. V79 cells were exposed to serial dilutions of the extracts of each sealer for 24 h. Cell viability was measured by the MTT assay and genotoxicity was assessed by the formation of micronuclei.
  
  
  RESULTS
  The single methacrylate-based sealer had the most cytotoxic effects, with significant reduction in cell viability in all dilutions of the extract. The castor oil polymer-based sealer was, on the other hand, the most biocompatible sealer, with no cytotoxic effects at any concentration. All tested sealers were not genotoxic, excepting the single methacrylate-based sealer.
  
  
  CONCLUSIONS
  The tested natural resin-based sealers presented low cytotoxic and no genotoxic effects on cell cultures.
  
  
  CONCLUSIONS
  These results may suggest a good alternative to develop new endodontic sealers, in order to achieve better biological response and healing, when compared to commercially available sealers.
authors: Department of Restorative Dentistry, Institute of Science and Technology, Univ. Estadual Paulista, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
authors: Gleyce O
authors: GO
authors: Silva
authors: Department of Restorative Dentistry, Institute of Science and Technology, Univ. Estadual Paulista, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil.|Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
authors: Bruno N
authors: BN
authors: Cavalcanti
authors: Department of Restorative Dentistry, Institute of Science and Technology, Univ. Estadual Paulista, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
authors: Tatiana R
authors: TR
authors: Oliveira
authors: Department of Restorative Dentistry, Institute of Science and Technology, Univ. Estadual Paulista, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
authors: Claudia V
authors: CV
authors: Bin
authors: Department of Biosciences, Institute of Science and Technology, Univ. Estadual Paulista (UNESP), São José dos Campos, Brazil.
authors: Samira E A
authors: SE
authors: Camargo
authors: Department of Restorative Dentistry, Institute of Science and Technology, Univ. Estadual Paulista, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil. henrique@fosjc.unesp.br.
authors: Carlos H R
authors: CH
authors: Camargo
mesh_terms: D004249:DNA Damage; D004853:Epoxy Resins; D006801:Humans; D008689:Methacrylates / Q000633:toxicity*; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials / Q000633:toxicity*
chemical_list: D004853:Epoxy Resins; D008689:Methacrylates; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials
keywords: Biocompatibility; Cytotoxicity; Genotoxicity; Root canal sealer