title: 3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control.
abstract: Of great interest to modern medicine and biomedical research is the ability to inject individual target cells with the desired genes or drug molecules. Some advances in cell electroporation allow for high throughput, high cell viability, or excellent dosage control, yet no platform is available for the combination of all three. In an effort to solve this problem, here we show a "3D nano-channel electroporation (NEP) chip" on a silicon platform designed to meet these three criteria. This NEP chip can simultaneously deliver the desired molecules into 40,000 cells per cm(2) on the top surface of the device. Each 650 nm pore aligns to a cell and can be used to deliver extremely small biological elements to very large plasmids (>10 kbp). When compared to conventional bulk electroporation (BEP), the NEP chip shows a 20 fold improvement in dosage control and uniformity, while still maintaining high cell viability (>90%) even in cells such as cardiac cells which are characteristically difficult to transfect. This high-throughput 3D NEP system provides an innovative and medically valuable platform with uniform and reliable cellular transfection, allowing for a steady supply of healthy, engineered cells.
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu and Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Lingqian
authors: L
authors: Chang
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu and Department of Electrical and Computer Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Paul
authors: P
authors: Bertani
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu.
authors: Daniel
authors: D
authors: Gallego-Perez
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu.
authors: Zhaogang
authors: Z
authors: Yang
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu.
authors: Feng
authors: F
authors: Chen
authors: Department of Internal Medicine, Ohio State University, Columbus, OH 43209, USA.
authors: Chiling
authors: C
authors: Chiang
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu.
authors: Veysi
authors: V
authors: Malkoc
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu.
authors: Tairong
authors: T
authors: Kuang
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu.
authors: Keliang
authors: K
authors: Gao
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu and Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors: L James
authors: LJ
authors: Lee
authors: National Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43210, USA. lu.173@osu.edu lee.31@osu.edu and Department of Electrical and Computer Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Wu
authors: W
authors: Lu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D051379:Mice; D014162:Transfection / Q000295:instrumentation* / Q000379:methods*