title: Carboxylic monolayer formation for observation of intracellular structures in HeLa cells with direct electron beam excitation-assisted fluorescence microscopy.
abstract: Intracellular structures of HeLa cells are observed using a direct electron beam excitation-assisted fluorescence (D-EXA) microscope. In this microscope, a silicon nitride membrane is used as a culture plate, which typically has a low biocompatibility between the sample and the silicon nitride surface to prevent the HeLa cells from adhering strongly to the surface. In this work, the surface of silicon nitride is modified to allow strong cell attachment, which enables high-resolution observation of intracellular structures and an increased signal-to-noise ratio. In addition, the penetration depth of the electron beam is evaluated using Monte Carlo simulations. We can conclude from the results of the observations and simulations that the surface modification technique is promising for the observation of intracellular structures using the D-EXA microscope.
authors: Shizuoka University Graduate School of Science and Technology, 3-5-1, Johoku, Naka, Hamamatsu 432-8561, Japan.
authors: Yuriko
authors: Y
authors: Masuda
authors: Shizuoka University Research Institute of Electrics, 3-5-1, Johoku, Naka, Hamamatsu 432-8561, Japan.
authors: Yasunori
authors: Y
authors: Nawa
authors: Shizuoka University Research Institute of Electrics, 3-5-1, Johoku, Naka, Hamamatsu 432-8561, Japan ; CREST, Japan Science and Technology Agency, Japan.
authors: Wataru
authors: W
authors: Inami
authors: Shizuoka University Research Institute of Electrics, 3-5-1, Johoku, Naka, Hamamatsu 432-8561, Japan ; CREST, Japan Science and Technology Agency, Japan.
authors: Yoshimasa
authors: Y
authors: Kawata
keywords: (170.0170) Medical optics and biotechnology; (170.0180) Microscopy