title: Rapid screening and identification of compounds with DNA-binding activity from Folium Citri Reticulatae using on-line HPLC-DAD-MS(n) coupled with a post column fluorescence detection system.
abstract: To study the interactions between natural compounds and deoxyribonucleic acid (DNA), a method has been established combining a high-performance liquid chromatography-diode array detector-multi-stage mass spectrometer with a fluorescence detector (HPLC-DAD-MS(n)-FLD). The FLD was used to monitor fluorescence intensity of the ethidium bromide-DNA (EB-DNA) complex when a compound separated by HPLC was introduced. This novel method was used to simultaneously obtain the HPLC fingerprint, UV spectra, MS(n) fragments and DNA-binding activity profile of various components in Folium Citri Reticulatae. As a result, 35 compounds were identified, of which 25 were found in the extract of Folium Citri Reticulatae for the first time, and 33 compounds showed DNA-binding activities, with the most active being feruloylhexaric and p-coumaroylhexaric acids. In addition, the precision, stability and reproducibility of this method were validated by two positive controls, quercetin and hesperidin. This new on-line method is accurate, precise and reliable for further high-throughput screening of DNA-binding compounds from food samples and other complex matrices.
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: echo.huisheng@163.com.
authors: Qingrong
authors: Q
authors: Fu
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Cangman
authors: C
authors: Zhang
authors: Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors: Zongtao
authors: Z
authors: Lin
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Hongyang
authors: H
authors: Sun
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Yi
authors: Y
authors: Liang
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Haixiu
authors: H
authors: Jiang
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Zhiling
authors: Z
authors: Song
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: hw9505@bjmu.edu.cn.
authors: Hong
authors: H
authors: Wang
authors: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: chenshizhong66@163.com.
authors: Shizhong
authors: S
authors: Chen
mesh_terms: D000146:Acids, Carbocyclic / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005453:Fluorescence; D006569:Hesperidin / Q000032:analysis; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000146:Acids, Carbocyclic; D005419:Flavonoids; D010936:Plant Extracts; D004247:DNA; D011794:Quercetin; D006569:Hesperidin
keywords: DNA-binding activity; Feruloylhexaric acids; Flavonoids; Folium Citri Reticulatae; HPLC–DAD–MS(n)–EB–DNA–FLD; p-Coumaroylhexaric acids