title: A Root-Based Combination Supplement Containing Pueraria lobata and Rehmannia glutinosa and Exercise Preserve Bone Mass in Ovariectomized Rats Fed a High-Fat Diet.
abstract: The aim of this study was to evaluate the effects of a supplement containing Pueraria lobata/Rehmannia glutinosa (PR) root extracts on bone turnover in ovariectomized (OVX) rats (a model for postmenopausal osteoporosis). Female Sprague-Dawley rats (8 weeks old) were randomized into eight groups: sham-operated rats with low-fat control diet + vehicle, OVX rats with low-fat control diet + vehicle, OVX rats with high-fat diet (HFD) + vehicle, OVX rats with HFD + vehicle + exercise, OVX rats with HFD + PR (400 mg/kg body weight/day p.o.), OVX rats with HFD + PR + exercise, OVX rats with HFD + 17β-estradiol (0.5 mg/kg body weight/day p.o.), OVX rats with HFD + 17β-estradiol + exercise. Bone microarchitecture, bone turnover markers (e.g., plasma alkaline phosphatase and osteocalcin), expressions of osteogenic and resorptive gene markers in the bone were measured. Eight weeks of PR and/or aerobic exercise improved cortical microarchitecture of the femur and decreased markers of bone turnover and expression of skeletal osteoclastogenic genes in the femur. PR supplementation combined with exercise preserved bone loss induced by estrogen deficiency and should be investigated further as an alternative to hormone replacement therapy for preventing osteoporosis in postmenopausal women.
authors: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors: Hyang Mok
authors: HM
authors: Ok
authors: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors: Meron Regu
authors: MR
authors: Gebreamanuel
authors: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors: Sang A
authors: SA
authors: Oh
authors: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors: Hyejin
authors: H
authors: Jeon
authors: Department of Exercise Science, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors: Won Jun
authors: WJ
authors: Lee
authors: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea. orank@ewha.ac.kr.
authors: Oran
authors: O
authors: Kwon
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D016723:Bone Remodeling / Q000187:drug effects; D059305:Diet, High-Fat; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D015663:Osteoporosis, Postmenopausal / Q000517:prevention & control*; D010052:Ovariectomy; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D029908:Pueraria; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D032267:Rehmannia; D055114:X-Ray Microtomography
chemical_list: D010936:Plant Extracts
keywords: Exercise; Osteoporosis; Ovariectomy; Postmenopausal; Pueraria lobata; Rehmannia glutinosa