title: Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm.
abstract: Discovered in 1909 by Retzius and described mainly by morphology, the cytoplasmic droplet of sperm (renamed here the Hermes body) is conserved among all mammalian species but largely undefined at the molecular level. Tandem mass spectrometry of the isolated Hermes body from rat epididymal sperm characterized 1511 proteins, 43 of which were localized to the structure in situ by light microscopy and two by quantitative electron microscopy localization. Glucose transporter 3 (GLUT-3) glycolytic enzymes, selected membrane traffic and cytoskeletal proteins were highly abundant and concentrated in the Hermes body. By electron microscope gold antibody labelling, the Golgi trafficking protein TMED7/p27 localized to unstacked flattened cisternae of the Hermes body, as did GLUT-3, the most abundant protein. Its biogenesis was deduced through the mapping of protein expression for all 43 proteins during male germ cell differentiation in the testis. It is at the terminal step 19 of spermiogenesis that the 43 characteristic proteins accumulated in the nascent Hermes body.
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1.
authors: Catherine E
authors: CE
authors: Au
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7.
authors: Louis
authors: L
authors: Hermo
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1.
authors: Elliot
authors: E
authors: Byrne
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1.
authors: Jeffrey
authors: J
authors: Smirle
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1.
authors: Ali
authors: A
authors: Fazel
authors: Department of Biomedical Engineering, McGill University, Montreal, Quebec, Canada H3A 1A1.
authors: Robert E
authors: RE
authors: Kearney
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7.
authors: Charles E
authors: CE
authors: Smith
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7.
authors: Hojatollah
authors: H
authors: Vali
authors: Centre for Cellular Imaging, Sahlgrenska Academy at the University of Gothenburg, PO Box 435, 40530 Gothenburg, Sweden.
authors: Julia
authors: J
authors: Fernandez-Rodriguez
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1.
authors: Paul H G
authors: PH
authors: Simon
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7.
authors: Craig
authors: C
authors: Mandato
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1.
authors: Tommy
authors: T
authors: Nilsson
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 0C7 Department of Medicine, McGill University Health Centre Research Institute, 1001 Decarie Blvd, Montreal, Quebec, Canada H4A 3J1 Royal Victoria Hospital, Center for Translational Biology, RI-MUHC, Glen Site, 1001 Decarie Blvd, Bloc E, Room E02.7210, Montreal, Quebec, Canada H4A 3J1 john.bergeron@mcgill.ca.
authors: John J M
authors: JJ
authors: Bergeron
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement; D003593:Cytoplasm / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006056:Golgi Apparatus / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D010445:Peptide Elongation Factors / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism; D013087:Spermatids / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D000199:Actins; D008565:Membrane Proteins; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; D014404:Tubulin; D046988:Proteasome Endopeptidase Complex; D005947:Glucose
keywords: cryosection electron microscopy localization; light microscope localization; membrane traffic; quantitative proteomics; subcellular fractionation