title: Corticosteroid Therapy Benefits Septic Mice With Adrenal Insufficiency But Harms Septic Mice Without Adrenal Insufficiency.
abstract: OBJECTIVE
  Corticosteroid therapy is frequently used in septic patients given the rationale that there is an increased demand for corticosteroid in sepsis, and up to 60% of severe septic patients experience adrenal insufficiency. However, the efficacy of corticosteroid therapy and whether the therapy should be based on the results of adrenal function testing are highly controversial. The lack of an adrenal insufficiency animal model and our poor understanding of the pathogenesis caused by adrenal insufficiency present significant barriers to address this long-standing clinical issue.
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  Scavenger receptor BI null and adrenal-specific scavenger receptor BI null mice.
  
  
  METHODS
  Sepsis was induced by cecal ligation and puncture.
  
  
  RESULTS
  Using scavenger receptor BI mice as the first relative adrenal insufficiency animal model, we found that corticosteroid therapy significantly improved the survival in cecal ligation and puncture-treated scavenger receptor BI mice but causes more septic death in wild-type mice. We identified a corticosteroid cocktail that provides effective protection 18 hours post cecal ligation and puncture; using adrenal-specific scavenger receptor BI mice as an inducible corticosteroid-deficient animal model, we found that inducible corticosteroid specifically suppresses interleukin-6 production without affecting tumor necrosis factor-α, nitric oxide, and interleukin-10 production. We further found that inducible corticosteroid does not induce peripheral lymphocyte apoptosis but promotes phagocytic activity of macrophages and neutrophils.
  
  
  CONCLUSIONS
  This study demonstrates that corticosteroid treatment benefits mice with adrenal insufficiency but harms mice without adrenal insufficiency. This study also reveals that inducible corticosteroid has both immunosuppressive and immunopermissive properties, suppressing interleukin-6 production, promoting phagocytosis of immune effector cells, but not inducing peripheral lymphocyte apoptosis. These findings support our hypothesis that corticosteroid is an effective therapy for a subgroup of septic patients with adrenal insufficiency but harms septic patients without adrenal insufficiency and encourage further efforts to test this hypothesis in clinic.
authors: 1Department of Pediatrics, College of Medicine, University of Kentucky, Lexington, KY. 2Department of Pharmacology and Nutritional Sciences, College of Medicine, University of Kentucky, Lexington, KY. 3Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY. 4Kentucky Cancer Registry, College of Medicine, University of Kentucky, Lexington, KY.
authors: Junting
authors: J
authors: Ai
authors: Ling
authors: L
authors: Guo
authors: Zhong
authors: Z
authors: Zheng
authors: Shu-Xia
authors: SX
authors: Wang
authors: Bing
authors: B
authors: Huang
authors: Xiang-An
authors: XA
authors: Li
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use*; D000309:Adrenal Insufficiency / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011897:Random Allocation; D018570:Risk Assessment; D018805:Sepsis / Q000188:drug therapy* / Q000401:mortality / Q000503:physiopathology; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D000305:Adrenal Cortex Hormones