title: Investigation of temperature effect on cell mechanics by optofluidic microchips.
abstract: Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 - 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus.
authors: Dip. Ingegneria Industriale e dell'Informazione, Università di Pavia, Via Ferrata 5A, 27100 Pavia, Italy.
authors: Tie
authors: T
authors: Yang
authors: Dip. Ingegneria Industriale e dell'Informazione, Università di Pavia, Via Ferrata 5A, 27100 Pavia, Italy ; Dip. Biotecnologie Mediche e Medicina Traslazionale, Università di Milano,Via F.lli Cervi 91, 20090 Segrate, Italy.
authors: Giovanni
authors: G
authors: Nava
authors: Dip. Ingegneria Industriale e dell'Informazione, Università di Pavia, Via Ferrata 5A, 27100 Pavia, Italy.
authors: Paolo
authors: P
authors: Minzioni
authors: Istituto di Genetica Molecolare (IGM) - CNR, Via Abbiategrasso 207, 27100 Pavia, Italy.
authors: Manuela
authors: M
authors: Veglione
authors: Istituto di Fotonica e Nanotecnologie (IFN)-CNR,Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors: Francesca
authors: F
authors: Bragheri
authors: Dip. Ingegneria Industriale e dell'Informazione, Università di Pavia, Via Ferrata 5A, 27100 Pavia, Italy.
authors: Francesca Demetra
authors: FD
authors: Lelii
authors: Istituto di Fotonica e Nanotecnologie (IFN)-CNR,Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors: Rebeca Martinez
authors: RM
authors: Vazquez
authors: Istituto di Fotonica e Nanotecnologie (IFN)-CNR,Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors: Roberto
authors: R
authors: Osellame
authors: Dip. Ingegneria Industriale e dell'Informazione, Università di Pavia, Via Ferrata 5A, 27100 Pavia, Italy.
authors: Ilaria
authors: I
authors: Cristiani
keywords: (130.3120) Integrated optics devices; (140.6810) Thermal effects; (170.1530) Cell analysis; (170.4520) Optical confinement and manipulation