title: Fish collagen/alginate/chitooligosaccharides integrated scaffold for skin tissue regeneration application.
abstract: An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as a cross-linking agent. The effects of cross-linking were analyzed by Fourier transform infrared spectroscopy. The results indicate that the homogeneous materials blending and cross-linking intensity were dependent on the molecular weights of COSs. The highly interconnected porous architecture with 160-260μm pore size and over 90% porosity and COS's MW driven swelling and retention capacity, tensile property and in vitro biodegradation behavior guaranteed the FCA/COS scaffolds for skin tissue engineering application. Further improvement of these properties enhanced the cytocompatibility of all the scaffolds, especially the scaffolds containing COSs with MW in the range of 1-3kDa (FCA/COS1) showed the best cytocompatibility. These physicochemical, mechanical, and biological properties suggest that the FCA/COS1 scaffold is a superior candidate that can be used for skin tissue regeneration.
authors: Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Pathum
authors: P
authors: Chandika
authors: Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Seok-Chun
authors: SC
authors: Ko
authors: Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Gun-Woo
authors: GW
authors: Oh
authors: Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Seong-Yeong
authors: SY
authors: Heo
authors: Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Van-Tinh
authors: VT
authors: Nguyen
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: You-Jin
authors: YJ
authors: Jeon
authors: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors: Bonggi
authors: B
authors: Lee
authors: Department of Otolaryngology, Chonnam National University Medical School, Gwangju 500-757, Republic of Korea.
authors: Chul Ho
authors: CH
authors: Jang
authors: Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors: GeunHyung
authors: G
authors: Kim
authors: Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors: Won Sun
authors: WS
authors: Park
authors: Departments of Anesthesiology and Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.
authors: Wonseok
authors: W
authors: Chang
authors: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea; Department of Microbiology, College of Medicine, Inje University, Busan 614-735, Republic of Korea. Electronic address: cihima@inje.ac.kr.
authors: Il-Whan
authors: IW
authors: Choi
authors: Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: wkjung@pknu.ac.kr.
authors: Won-Kyo
authors: WK
authors: Jung
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D003094:Collagen / Q000737:chemistry*; D005399:Fishes; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008422:Materials Testing; D009844:Oligosaccharides; D012038:Regeneration; D012867:Skin; D017768:Skin, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D009844:Oligosaccharides; C493484:oligochitosan; D002686:Chitin; D020723:Glucuronic Acid; D003094:Collagen; D048271:Chitosan
keywords: Alginate; Chitooligosaccharides; Fish collagen; Skin tissue engineering