title: Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping.
abstract: We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca(2+) level at the individual-cell level.
authors: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Jonghee
authors: J
authors: Yoon
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Minji
authors: M
authors: Lee
authors: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: KyeoReh
authors: K
authors: Lee
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Nury
authors: N
authors: Kim
authors: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Jin Man
authors: JM
authors: Kim
authors: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Jongchan
authors: J
authors: Park
authors: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Hyeonseung
authors: H
authors: Yu
authors: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: Chulhee
authors: C
authors: Choi
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.|Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 305-811, Republic of Korea.
authors: Won Do
authors: WD
authors: Heo
authors: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors: YongKeun
authors: Y
authors: Park
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D062308:Optogenetics / Q000379:methods*; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism; D012542:Scattering, Radiation; D015398:Signal Transduction; D012886:Skull / Q000378:metabolism*
chemical_list: D051496:Receptor, Fibroblast Growth Factor, Type 1; D002118:Calcium