title: Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens.
abstract: We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs was sufficient to predict ciprofloxacin resistance in an organism.
authors: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: S W
authors: SW
authors: Peterson
authors: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: I
authors: I
authors: Martin
authors: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: W
authors: W
authors: Demczuk
authors: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: A
authors: A
authors: Bharat
authors: British Columbia Centres for Disease Control Public Health Microbiology & Reference Laboratory, Vancouver, British Columbia, Canada.
authors: L
authors: L
authors: Hoang
authors: Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
authors: J
authors: J
authors: Wylie
authors: Public Health Ontario Laboratories, Toronto, Ontario, Canada.
authors: V
authors: V
authors: Allen
authors: Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada.
authors: B
authors: B
authors: Lefebvre
authors: Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: G
authors: G
authors: Tyrrell
authors: Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada.
authors: G
authors: G
authors: Horsman
authors: Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada.
authors: D
authors: D
authors: Haldane
authors: Dr. G. L. Dumont Hospital, Moncton, New Brunswick, Canada.
authors: R
authors: R
authors: Garceau
authors: Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada.
authors: T
authors: T
authors: Wong
authors: Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada michael.mulvey@phac-aspc.gc.ca.
authors: M R
authors: MR
authors: Mulvey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002170:Canada; D002939:Ciprofloxacin / Q000494:pharmacology*; D003429:Cross Reactions; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006069:Gonorrhea / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase