title: Multi-residue analysis of veterinary drugs, pesticides and mycotoxins in dairy products by liquid chromatography-tandem mass spectrometry using low-temperature cleanup and solid phase extraction.
abstract: A multi-class multi-residue analysis method for determination of veterinary drugs, pesticides and mycotoxins in dairy products by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been established. These 17 classes, a total of 40 kinds of target compounds were chosen because their administration to food-producing animals is banned or regulated in China and may be potentially abused or misused. Samples were extracted with acetonitrile-ethyl acetate-acetic acid (49.5+49.5+1, v/v/v). Most of lipids in the extract were removed by low-temperature cleanup, prior to solid phase extraction on HLB cartridges. The quantification and confirmation of the 40 analytes were performed by LC-MS/MS with electro-spray ionization (ESI) interface in multiple reaction monitoring (MRM) mode. The limits of detection (LODs) and limits of quantification (LOQs) were 0.006-0.3μg/kg and 0.02-1.0μg/kg, respectively. The spiked recoveries in milk, yogurt, milk powder and cheese samples were from 67.3% to 106.9%. The repeatability and the within-laboratory reproducibility were less than 12.7% and 13.9%. Applying this method, our results revealed the presences of chloramphenicol, cimeterol, and flunixin at the concentration of 0.027-0.452μg/kg in some samples.
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Jie
authors: J
authors: Xie
authors: Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors: Tao
authors: T
authors: Peng
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Ailing
authors: A
authors: Zhu
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China.
authors: Jianli
authors: J
authors: He
authors: Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors: Qiaoying
authors: Q
authors: Chang
authors: Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors: Xueyan
authors: X
authors: Hu
authors: Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors: Hui
authors: H
authors: Chen
authors: Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors: Chunlin
authors: C
authors: Fan
authors: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China.
authors: Wenxiao
authors: W
authors: Jiang
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Min
authors: M
authors: Chen
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Jiancheng
authors: J
authors: Li
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Shuangyang
authors: S
authors: Ding
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: haiyang@cau.edu.cn.
authors: Haiyang
authors: H
authors: Jiang
mesh_terms: D002681:China; D002853:Chromatography, Liquid / Q000379:methods*; D003080:Cold Temperature; D003611:Dairy Products / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014730:Veterinary Medicine
chemical_list: D009183:Mycotoxins; D010573:Pesticide Residues
keywords: Dairy products; LC–MS/MS; Low-temperature cleanup; Mycotoxins; Pesticides; Veterinary drugs