title: Upconversion luminescence resonance energy transfer-based aptasensor for the sensitive detection of oxytetracycline.
abstract: In this work, a biosensor based on luminescence resonance energy transfer (LRET) from NaYF4:Yb,Tm upconversion nanoparticles (UCNPs) to SYBR Green I has been developed. The aptamers are covalently linked to UCNPs and hybridized with their complementary strands. The subsequent addition of SYBR Green allows SYBR Green I to insert into the formed double-stranded DNA (dsDNA) duplex and brings the energy donor and acceptor into close proximity, leading to the fluorescence of UCNPs transferred to SYBR Green I. When excited at 980 nm, the UCNPs emit luminescence at 477 nm, and this energy is transferred to SYBR Green I, which emits luminescence at 530 nm. In the presence of oxytetracycline (OTC), the aptamers prefer to bind to its corresponding analyte and dehybridize with the complementary DNA. This dehybridization leads to the liberation of SYBR Green I, which distances SYBR Green I from the UCNPs and recovers the UCNPs' luminescence. Under optimal conditions, a linear calibration is obtained between the ratio of I530 to I477 nm (I530/I477) and the OTC concentration, which ranges from 0.1 to 10 ng/ml with a limit of detection (LOD) of 0.054 ng/ml.
authors: China Rural Technology Development Center, Beijing 100045, China.
authors: Hui
authors: H
authors: Zhang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China.
authors: Congcong
authors: C
authors: Fang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: wusj1986@163.com.
authors: Shijia
authors: S
authors: Wu
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China.
authors: Nuo
authors: N
authors: Duan
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp1974@163.com.
authors: Zhouping
authors: Z
authors: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D052160:Benzothiazoles; D060807:Bioluminescence Resonance Energy Transfer Techniques; D015374:Biosensing Techniques; D002138:Calibration; D002681:China; D004274:DNA, Recombinant / Q000737:chemistry; D003959:Diamines; D004350:Drug Residues / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry; D005513:Food Inspection / Q000379:methods*; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009930:Organic Chemicals / Q000737:chemistry; D010118:Oxytetracycline / Q000032:analysis*; D011804:Quinolines; D015203:Reproducibility of Results; D013499:Surface Properties; D013932:Thulium / Q000737:chemistry; D015018:Ytterbium / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D052160:Benzothiazoles; D004274:DNA, Recombinant; D003959:Diamines; D005456:Fluorescent Dyes; D061925:Immobilized Nucleic Acids; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D013932:Thulium; D015018:Ytterbium; D010118:Oxytetracycline
keywords: Aptamer; Luminescence resonance energy transfer; Oxytetracycline; Upconversion nanoparticles