title: Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans.
abstract: Luciferase, a bioluminescent protein, has been used as an analytical tool to visualize intracellular phenomena. Luciferase with red light emission is particularly useful for bioluminescence imaging because of its high transmittance in mammalian tissues. However, the luminescence intensity of existing luciferases with their emission over 600 nm is insufficient for imaging studies because of their weak intensities. We developed mutants of Emerald luciferase (Eluc) from Brazilian click beetle (Pyrearinus termitilluminans), which emits the strongest bioluminescence among beetle luciferases. We successively introduced four amino acid mutations into the luciferase based on a predicted structure of Eluc using homology modeling. Results showed that quadruple mutations R214K/H241K/S246H/H347A into the beetle luciferase emit luminescence with emission maximum at 626 nm, 88-nm red-shift from the wild-type luciferase. This mutant luciferase is anticipated for application in in vivo multicolor imaging in living samples.
authors: Tomoki
authors: T
authors: Nishiguchi
authors: Toshimichi
authors: T
authors: Yamada
authors: Yusuke
authors: Y
authors: Nasu
authors: Mashiho
authors: M
authors: Ito
authors: Hideaki
authors: H
authors: Yoshimura
authors: Takeaki
authors: T
authors: Ozawa
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000737:chemistry*; D003116:Color; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*
chemical_list: D008156:Luciferases