title: Isofuranodiene: A neuritogenic compound isolated from wild celery (Smyrnium olusatrum L., Apiaceae).
abstract: In the search for neuroactive compounds that mimic the nerve growth factor (NGF) activity for the protection against neurodegenerative diseases, the potential medicinal values of foods and plants attracts intense interest. Isofuranodiene is the major constituent of the essential oil of wild celery (Smyrnium olusatrum L., Apiaceae). The cytotoxic effects of isofuranodiene towards rat neuronal PC-12 pheochromocytoma cells were determined by MTT assay, while the cell differentiation was evaluated with xCELLigence real time cell analysis system (RTCA DP), and the neuritogenic activity was assessed by neurite outgrowth image analysis. Isofuranodiene at concentrations of 25 and 12.5 μM alone, or in combination with 50 nM NGF, showed a marked stimulation of neuritogenesis, but it was more effective at 12.5 μM with or without NGF. The present study reports the first evidence of the neuritogenic effects of isofuranodiene, which appears to be a promising neurotrophic and neuroprotective agent deserving further investigation.
authors: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors: Ahmed M
authors: AM
authors: Mustafa
authors: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy. Electronic address: filippo.maggi@unicam.it.
authors: Filippo
authors: F
authors: Maggi
authors: School of Science and Technology, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors: Fabrizio
authors: F
authors: Papa
authors: Department of Pharmacology, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
authors: Elif
authors: E
authors: Kaya
authors: Department of Pharmacology, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
authors: Miris
authors: M
authors: Dikmen
authors: Department of Pharmacology, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
authors: Yusuf
authors: Y
authors: Öztürk
mesh_terms: D000818:Animals; D019661:Apiaceae; D000077102:Apium / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005663:Furans / Q000032:analysis / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted; D020932:Nerve Growth Factor / Q000378:metabolism; D018696:Neuroprotective Agents; D009822:Oils, Volatile / Q000032:analysis; D016716:PC12 Cells; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D051381:Rats
chemical_list: D005663:Furans; D018696:Neuroprotective Agents; D009822:Oils, Volatile; D010936:Plant Extracts; C017897:isofuranodiene; D020932:Nerve Growth Factor
keywords: Isofuranodiene; Isofuranodiene (PubChem CID: 5317424); Nerve growth factor; Neurite outgrowth image analysis; Neuritogenic activity; PC-12 cells; Smyrnium olusatrum L.; xCELLigence RTCA DP system