title: Effects of dipotassium-trioxohydroxytetrafluorotriborate, K2[B3O3F4OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line.
abstract: Recently it was found that dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), is a potent and highly specific inhibitor of precancerous cell processes. We conducted gene expression profiling of human melanoma cells before and after treatment with two concentrations (0.1 and 1 mM) of this boron inorganic derivative in order to assess its effects on deregulation of genes associated with tumor pathways. Parallel trypan blue exclusion assay was performed to assess the cytotoxicity effects of this chemical. Treatment with K2(B3O3F4OH) induced a significant decrease of cell viability in melanoma cellline at both tested concentrations. Furthermore, these treatments caused deregulation of more than 30 genes known as common anti-tumor drug targets. IGF-1 and hTERT were found to be significantly downregulated and this result may imply potential use of K2(B3O3F4OH) as an inhibitor or human telomerase and insulin-like growth factor 1, both of which are associated with various tumor pathways.
authors: a Institute for Genetic Engineering and Biotechnology, University of Sarajevo , Sarajevo, Bosnia and Herzegovina .
authors: Lejla
authors: L
authors: Pojskic
authors: a Institute for Genetic Engineering and Biotechnology, University of Sarajevo , Sarajevo, Bosnia and Herzegovina .
authors: Sanin
authors: S
authors: Haveric
authors: a Institute for Genetic Engineering and Biotechnology, University of Sarajevo , Sarajevo, Bosnia and Herzegovina .
authors: Naida
authors: N
authors: Lojo-Kadric
authors: a Institute for Genetic Engineering and Biotechnology, University of Sarajevo , Sarajevo, Bosnia and Herzegovina .
authors: Maida
authors: M
authors: Hadzic
authors: a Institute for Genetic Engineering and Biotechnology, University of Sarajevo , Sarajevo, Bosnia and Herzegovina .
authors: Anja
authors: A
authors: Haveric
authors: b David Geffen School of Medicine at UCLA, University of Los Angeles , Los Angeles , CA , USA .
authors: Zoran
authors: Z
authors: Galic
authors: c Department of Chemistry, Faculty of Science , University of Sarajevo , Sarajevo, Bosnia and Herzegovina .
authors: Borivoj
authors: B
authors: Galic
authors: d Laboratorio di Chimica Bioinorganica , Polo Scientifico, degli Studi di Firenze , Firenze , Italy .
authors: Daniela
authors: D
authors: Vullo
authors: e Neurofarba Department, Section of Pharmaceutical and Nutriceutical Sciences, Università degli Studi di Firenze , Firenze , Italy , and.
authors: Claudiu T
authors: CT
authors: Supuran
authors: f Department of Biochemistry, Faculty of Chemistry and Technology , University of Split , Split , Croatia.
authors: Mladen
authors: M
authors: Milos
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D008545:Melanoma / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001896:Boron Compounds; C561431:dipotassium trioxohydroxytetrafluorotriborate; D007334:Insulin-Like Growth Factor I; C509186:TERT protein, human; D019098:Telomerase
keywords: Antitumor drug target; dipotassium-trioxohydroxytetrafluorotriborate; gene down-regulation; gene expression; human melanoma cell line; human telomerase; insulin-like growth factor 1