title: Acoustophoretic removal of proteins from blood components.
abstract: This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97%, compared to just above 90% of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.
authors: Department of Biomedical Engineering, Lund University, Lund, Sweden, maria.tenje@angstrom.uu.se.
authors: Maria
authors: M
authors: Tenje
authors: Maria N
authors: MN
authors: Lundgren
authors: Ann-Margret
authors: AM
authors: Swärd-Nilsson
authors: Jens
authors: J
authors: Kjeldsen-Kragh
authors: Lena
authors: L
authors: Lyxe
authors: Andreas
authors: A
authors: Lenshof
mesh_terms: D000162:Acoustics / Q000295:instrumentation; D001781:Blood Component Removal / Q000295:instrumentation*; D001798:Blood Proteins / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000737:chemistry* / Q000528:radiation effects; D019220:High-Energy Shock Waves; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008904:Miniaturization; D013010:Sonication / Q000295:instrumentation*
chemical_list: D001798:Blood Proteins