title: ANGUSTIFOLIA mediates one of the multiple SCRAMBLED signaling pathways regulating cell growth pattern in Arabidopsis thaliana.
abstract: In Arabidopsis thaliana, an atypical leucine-rich repeat receptor-like kinase, SCRAMBLED (SCM), is required for multiple developmental processes including root epidermal cell fate determination, silique dehiscence, inflorescence growth, ovule morphogenesis, and tissue morphology. Previous work suggested that SCM regulates these multiple pathways using distinct mechanisms via interactions with specific downstream factors. ANGUSTIFOLIA (AN) is known to regulate cell and tissue morphogenesis by influencing cortical microtubule arrangement, and recently, the AN protein was reported to interact with the SCM protein. Therefore, we examined whether AN might be responsible for mediating some of the SCM-dependent phenotypes. We discovered that both scm and an mutant lines cause an abnormal spiral or twisting growth of roots, but only the scm mutant affected root epidermal patterning. The siliques of the an and scm mutants also exhibited spiral growth, as previously reported, but only the scm mutant altered silique dehiscence. Interestingly, we discovered that the spiral growth of roots and siliques of the scm mutant is rescued by a truncated SCM protein that lacks its kinase domain, and that a juxtamembrane domain of SCM was sufficient for AN binding in the yeast two-hybrid analysis. These results suggest that the AN protein is one of the critical downstream factors of SCM pathways specifically responsible for mediating its effects on cell/tissue morphogenesis through cortical microtubule arrangement.
authors: Department of Biology, Long Island University, 1 University Plaza, Brooklyn, NY, USA. Electronic address: su-hwan.kwak@liu.edu.
authors: Su-Hwan
authors: SH
authors: Kwak
authors: Department of Biology, Chosun University, Gwangju 501-759, Republic of Korea.
authors: Sang-Kee
authors: SK
authors: Song
authors: Department of Systems Biology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors: Myeong Min
authors: MM
authors: Lee
authors: Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Avenue, Ann Arbor, MI, USA.
authors: John
authors: J
authors: Schiefelbein
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology; D018517:Plant Roots / Q000502:physiology*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D020794:Receptor Protein-Tyrosine Kinases; D012097:Repressor Proteins; C452846:DETORQEO protein, Arabidopsis; C503317:SUB protein, Arabidopsis
keywords: ANGUSTIFOLIA (AN); Arabidopsis thaliana; Leucine-rich repeat receptor-like kinase; Root development; SCRAMBLED (SCM); Silique development