title: PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.
abstract: Human PIWIL1, alias HIWI, is a member of Piwi protein family and expressed in various tumors. However, the underlying mechanism of PIWIL1 in tumorigenesis remains largely unknown. Stathmin1 is a cytosolic phosphoprotein which has a critical role in regulating microtubule dynamics and is overexpressed in many cancers. Here we report that PIWIL1 can directly bind to Stathmin1. Meanwhile, PIWIL1 can up-regulate the expression of Stathmin1 through inhibiting ubiquitin-mediated degradation induced by an E3 ubiquitin ligase RLIM. Furthermore, PIWIL1 can also reduce phosphorylation level of Stathmin1 at Ser-16 through inhibiting the interaction between CaMKII and Stathmin1. Our results showed that PIWIL1 suppresses microtubule polymerization, and promotes cell proliferation and migration via Stathmin1 for the first time. Our study reveals a novel mechanism for PIWIL1 in tumorigenesis.
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Chao
authors: C
authors: Li
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Xiaoyan
authors: X
authors: Zhou
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Jianhui
authors: J
authors: Chen
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Yilu
authors: Y
authors: Lu
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Qianqian
authors: Q
authors: Sun
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Dachang
authors: D
authors: Tao
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Wei
authors: W
authors: Hu
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Xulei
authors: X
authors: Zheng
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Shasha
authors: S
authors: Bian
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Yunqiang
authors: Y
authors: Liu
authors: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors: Yongxin
authors: Y
authors: Ma
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism*; D015153:Blotting, Western; D063646:Carcinogenesis / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047468:Immunoprecipitation; D008870:Microtubules / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology; D009369:Neoplasms / Q000473:pathology; D010766:Phosphorylation; D060888:Real-Time Polymerase Chain Reaction; D012694:Serine / Q000378:metabolism; D050777:Stathmin / Q000378:metabolism*; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; C421088:PIWIL1 protein, human; C493995:STMN1 protein, human; D050777:Stathmin; D012694:Serine; C492370:RLIM protein, human; D044767:Ubiquitin-Protein Ligases
keywords: PIWIL1; STMN1; migration; proliferation; tubulin