title: Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry.
abstract: In this present study, we developed a simple, rapid and specific method for the quantitative analysis of the contents of amoxicillin (AMO), AMO metabolites and ampicillin (AMP) in eggs. This method uses a simple liquid-liquid extraction with acetonitrile followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The optimized method has been validated according to requirements defined by the European Union and Food and Drug Administration. Extraction recoveries of the target compounds from the egg at 5, 10 and 25 μg/kg were all higher than 80%, with relative standard deviations not exceeding 10.00%. The limits of quantification in eggs were below the maximum residue limits (MRLs). The decision limits (CCα) ranged between 11.1 and 11.5 μg/kg, while detection capabilities (CCβ) from 12.1 to 13.0 μg/kg. These values were very close to the corresponding MRLs. Finally, the new approach was successfully verified for the quantitative determination of these analytes in 40 commercial eggs from local supermarkets.
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Lirui
authors: L
authors: Sun
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors: Longfei
authors: L
authors: Jia
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors: Xing
authors: X
authors: Xie
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. Electronic address: yzxkz168@163.com.
authors: Kaizhou
authors: K
authors: Xie
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Jianfeng
authors: J
authors: Wang
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Jianyu
authors: J
authors: Liu
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Lulu
authors: L
authors: Cui
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Genxi
authors: G
authors: Zhang
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Guojun
authors: G
authors: Dai
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China.
authors: Jinyu
authors: J
authors: Wang
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000658:Amoxicillin / Q000032:analysis* / Q000378:metabolism; D000667:Ampicillin / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D059625:Liquid-Liquid Extraction; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014481:United States; D019155:Veterinary Drugs / Q000032:analysis* / Q000378:metabolism
chemical_list: D000097:Acetonitriles; D019155:Veterinary Drugs; D000667:Ampicillin; D000658:Amoxicillin; C032159:acetonitrile
keywords: Acetonitrile (PubChem CID:6342); Ammonium acetate (PubChem CID:517165); Amoxicillin; Amoxicillin (PubChem CID:33613); Amoxicillin diketopiperazine-2′,5′-dione; Amoxicillin diketopiperazine-2′,5′-dione (PubChem CID:46780379); Amoxicilloic acid; Amoxicilloic acid (PubChem CID:71313389); Ampicillin; Ampicillin (PubChem CID:6249); Glacial acetic acid (PubChem CID:176); LC–MS/MS; Penicillin V (PubChem CID:6869); Residues in eggs