title: LC/MS-based polar metabolite profiling reveals gender differences in serum from patients with myocardial infarction.
abstract: Myocardial infarction (MI), a leading cause of death worldwide, results from prolonged myocardial ischemia with necrosis of myocytes due to a blood supply obstruction to an area of the heart. Many studies have reported gender-related differences in the clinical features of MI, but the reasons for these differences remain unclear. In this study, we applied ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) and various statistical methods-such as multivariate, pathway, and correlation analyses-to identify gender-specific metabolic patterns in polar metabolites in serum from healthy individuals and patients with MI. Patients with diagnosed MI (n=68), and age- and body mass index-matched healthy individuals (n=68), were included in this study. The partial least-squares discriminant analysis (PLS-DA) model was generated from metabolic profiling data, and the score plots showed a significant gender-related difference in patients with MI. Many pathways were associated with amino acids and purines; amino acids, acylcarnitines, and purines differed significantly between male and female patients with MI. This approach could be utilized to observe gender-specific metabolic pattern differences between healthy controls and patients with MI.
authors: Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute (KBSI), Seoul 120-140,Republic of Korea; Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
authors: Jueun
authors: J
authors: Lee
authors: Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute (KBSI), Seoul 120-140,Republic of Korea.
authors: Youngae
authors: Y
authors: Jung
authors: Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute (KBSI), Seoul 120-140,Republic of Korea.
authors: Ju Yeon
authors: JY
authors: Park
authors: Cardiology Division, Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors: Sang-Hak
authors: SH
authors: Lee
authors: Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea. Electronic address: dhryu@skku.edu.
authors: Do Hyun
authors: DH
authors: Ryu
authors: Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute (KBSI), Seoul 120-140,Republic of Korea; Department of Life Science, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: gshwang@kbsi.re.kr.
authors: Geum-Sook
authors: GS
authors: Hwang
mesh_terms: D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D012737:Sex Factors
chemical_list: D015415:Biomarkers
keywords: Gender-related differences; Metabolic profiling; Myocardial infarction; UPLC/Q-TOF MS