title: Lutein prevents alcohol-induced liver disease in rats by modulating oxidative stress and inflammation.
abstract: OBJECTIVE
  Oxidative stress and inflammation play an important role in pathogenesis of alcohol-induced liver injury. The present study was designed to investigate the protective role of Lutein against alcohol-induced liver injury.
  
  
  METHODS
  Wistar rats weighing 150-200 g were divided into 3 groups, control, EtOH treatment, Lutein followed by EtOH treatment. Ethanol-treated rats received EtOH [5 g/kg body weight] by gavage every 12 hours for a total of 3 doses. For Lutein pre-treatment, Lutein at a dose of 40 mg/kg was dissolved in the EtOH and gavaged 30 mins before EtOH treatment.
  
  
  METHODS
  Oxidative stress markers-(reactive oxygen species, lipid peroxidation, protein carbonyls and sulfhydryls content), liver markers (ALT, AST, ALP and LDH) were determined. Antioxidant enzyme activities and its master regulator Nrf-2 expression were analyzed. Further, inflammatory proteins NF-κB, COX-2, iNOS and inflammatory cytokines (TNF-α, MCP-1, IL-1β, IL-6) were analyzed.
  
  
  RESULTS
  The results showed significant decrease in oxidative stress markers and liver markers in the lutein pre-treatment. Lutein treatment down regulated inflammatory proteins and cytokines with concomitant up regulation in Nrf-2 levels and antioxidant enzymic activities.
  
  
  CONCLUSIONS
  The present study showed that Lutein treatment exerted potent antioxidant and anti-inflammatory property and offered significant cytoprotection against alcohol-induced liver injury.
authors: Department of Gastroenterology, China-Japan Friendship Hospital Beijing 100029, China.
authors: Shi-Yu
authors: SY
authors: Du
authors: Department of Gastroenterology, China-Japan Friendship Hospital Beijing 100029, China.
authors: Yan-Li
authors: YL
authors: Zhang
authors: Department of Gastroenterology, China-Japan Friendship Hospital Beijing 100029, China.
authors: Ru-Xue
authors: RX
authors: Bai
authors: Department of Gastroenterology, Beijing Ditan Hospital, Capital Medical University Beijing 100015, China.
authors: Zheng-Lin
authors: ZL
authors: Ai
authors: Department of Gastroenterology, The First Affiliated Hospital of Nanchang University Nanchang 150001, China.
authors: Bu-Shan
authors: BS
authors: Xie
authors: Department of Gastroenterology, China-Japan Friendship Hospital Beijing 100029, China.
authors: Hua-Yuan
authors: HY
authors: Yang
keywords: Alcohol; cytokines; inflammation; liver injury; lutein; oxidative stress