title: Effect of Bromine Substitution on Human Dermal Absorption of Polybrominated Diphenyl Ethers.
abstract: Human dermal absorption of eight mono- to deca-brominated diphenyl ethers (PBDEs) was investigated for the first time using EPISKIN human skin equivalent tissue. Using a standard in vitro protocol, EPISKIN tissues mounted in specially designed diffusion cells were exposed to the target PBDEs for 24 h. Estimated steady-state flux (Jss) and permeation coefficients (Papp) across the skin increased with decreasing bromine substitution from BDE-153 (Papp = 4.0 × 10(-4) cm/h) to BDE-1 (Papp = 1.1 × 10(-2) cm/h). This was accompanied by an increase in the time required to traverse the skin tissue into the receptor fluid (lag time) from 0.25 h for BDE-1 to 1.26 h for BDE-153. Papp values for the studied PBDEs were correlated significantly (P < 0.05) with physicochemical parameters like water solubility and log KOW. While less brominated congeners achieved faster dermal penetration, higher PBDEs displayed greater accumulation within the skin tissue. The PBDEs thus accumulated represent a contaminant depot from which they may be slowly released to the systemic circulation over a prolonged period. Maximal percutaneous penetration was observed for BDE-1 (∼ 30% of the applied 500 ng/cm(2) dose). Interestingly, BDE-183 and BDE-209 showed very low dermal absorption, exemplified by a failure to reach the steady state within the 24 h exposure period that was studied.
authors: Division of Environmental Health and Risk Management, School of Geography, Earth, and Environmental Sciences, University of Birmingham , Birmingham B15 2TT, United Kingdom.|Department of Analytical Chemistry, Faculty of Pharmacy, Assiut University , 71526 Assiut, Egypt.
authors: Mohamed Abou-Elwafa
authors: MA
authors: Abdallah
authors: Division of Environmental Health and Risk Management, School of Geography, Earth, and Environmental Sciences, University of Birmingham , Birmingham B15 2TT, United Kingdom.
authors: Gopal
authors: G
authors: Pawar
authors: Division of Environmental Health and Risk Management, School of Geography, Earth, and Environmental Sciences, University of Birmingham , Birmingham B15 2TT, United Kingdom.
authors: Stuart
authors: S
authors: Harrad
mesh_terms: D001966:Bromine / Q000737:chemistry*; D004781:Environmental Exposure; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010539:Permeability; D012869:Skin Absorption; D017768:Skin, Artificial
chemical_list: D055768:Halogenated Diphenyl Ethers; D001966:Bromine