title: [Effects of Garcinia Acid Combined with Daunorubicin on Expression of Pregnane X Receptor in Leukemia Cell Line K562/A02].
abstract: OBJECTIVE
  To explore the expression of PXR (Pregnane X receptor) in several malignant hematological cell lines, and to investigate the reversal effect of Gambogic acid (GA) on multi-drug resistance (MDR) of K562/A02 cell line and its reversal mechanism.
  
  
  METHODS
  Transcription of PXR was detected by real-time PCR in several malignant hematological cell lines. The growth inhibition rate of K562/A02 in different experimental groups was assayed by MTT method, and the expression of PXR protein was measured by Western blot.
  
  
  RESULTS
  PXR gene transcription could be detected in several hematological malignancy cell lines, and it was significantly higher in K562/A02 cell line, compared with the other cell lines used in this experiment. Low-dose GA could enhance cell growth inhibition rate, increasing the effect of chemotherapy, which may be associated with down-regulation of PXR expression. PXR gene transcription and protein expression in GA and DNR+GA groups decreased as compared with control group and the DNR group, suggesting that low-dose GA can down-regulate PXR gene transcription and protein expression.
  
  
  CONCLUSIONS
  PXR gene transcription can be detected in several hematological malignancy cell line, which is significantly higher in K562/A02 cell line, as compared with the other cell lines used in this experiment. Low-dose GA can enhance cell growth inhibition rate, increasing the effect of chemotherapy, which may be associated with down-regulation of PXR expression.
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Han
authors: H
authors: Zhu
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China. E-mail: cba8888@hotmail.com.
authors: Bao-An
authors: BA
authors: Chen
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Xiao-Hui
authors: XH
authors: Cai
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Fei
authors: F
authors: Wang
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Chong
authors: C
authors: Gao
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Jian
authors: J
authors: Cheng
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Xiao-Ping
authors: XP
authors: Zhang
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Lu
authors: L
authors: Dai
authors: Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors: Guo-Hua
authors: GH
authors: Xia
authors: Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China.
authors: Rong
authors: R
authors: Fu
authors: Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China.
authors: Qing-Long
authors: QL
authors: Guo
mesh_terms: D002951:Citrates; D003630:Daunorubicin; D015536:Down-Regulation; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D020014:K562 Cells; D007938:Leukemia; D000077297:Pregnane X Receptor; D060888:Real-Time Polymerase Chain Reaction; D011987:Receptors, Steroid; D044004:Xanthones
chemical_list: D002951:Citrates; D000077297:Pregnane X Receptor; D011987:Receptors, Steroid; D044004:Xanthones; C052659:gambogic acid; C007999:hydroxycitric acid; D003630:Daunorubicin