title: A new small molecule inhibits Streptococcus mutans biofilms in vitro and in vivo.
abstract: OBJECTIVE
  The aim of this study was to identify new small molecules that can inhibit Streptococcus mutans biofilms by in vitro and in vivo model.
  
  
  RESULTS
  We evaluated the effect of a small molecule 2-amino-imidazole/triazole conjugate (2-AI/T) on the formation of Strep. mutans biofilms by culturing in 96-well plates. Toxicity was assessed through cell culture and intragastrically administering to mice. The anti-biofilm and anti-caries effects were investigated in vivo. The inhibitive mechanism was detected by isobaric tag for relative and absolute quantification (itraq) and RT-QPCR. In vitro and in vivo study revealed that 2-AI/T significantly inhibited biofilm formation of Strep. mutans and is more so than inhibiting planktonic cells without toxicity. The ribosome and histidine metabolism pathways of Strep. mutans were significantly regulated by this compound.
  
  
  CONCLUSIONS
  These results suggest that the 2-AI/T conjugate is a potent inhibitor that can be potentially developed into a new drug to treat and prevent dental caries.
  
  
  CONCLUSIONS
  This is the first study to use small molecule from marine natural products, to protect from dental caries in vivo. It has potential broad range application in clinical caries prevention, or as a bioactive ingredient for food applications.
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: W
authors: W
authors: Pan
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: M
authors: M
authors: Fan
authors: Department of Pediatric Dentistry, UAB School of Dentistry, Birmingham, AL, USA.
authors: H
authors: H
authors: Wu
authors: Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
authors: C
authors: C
authors: Melander
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: C
authors: C
authors: Liu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000382:microbiology; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D013290:Streptococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology; D014230:Triazoles / Q000008:administration & dosage
chemical_list: D000900:Anti-Bacterial Agents; D007093:Imidazoles; D014230:Triazoles; C029899:imidazole
keywords: Streptococci; antimicrobials; biofilm; dental caries; small molecule