title: Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans.
abstract: In the study, an aqueous two phase system (ATPS) coupled with ultrasound was employed to extract lignans from Zanthoxylum armatum. Three standard lignans, namely (-)-fargesin, sesamin and L-asarinin, were used as marker compounds, and extraction was optimized and projected by response surface methodology (RSM) and artificial neural network (ANN). The optimal condition for ATPS with 20% n-propanol and 24% (NH4)2SO4 coupled with ultrasonic-assisted extraction including a solvent to solid ratio of 15:1, a temperature of 40 °C, and a treatment time of 55 min was obtained. Under the condition, the yield of (-)-fargesin increased 15.12%, and the purities of (-)-fargesin, sesamin and L-asarinin reached 2.222%, 1.066%, and 1.583%, with an increase of 44.38%, 25.70%, and 26.34% compared to those extracted with 95% ethanol, respectively. Coefficient of the determined (0.9855) and mean squared error (0.0018) of ANN model suggested good fitness and generalization of the ANN. Taken together, the results showed that ultrasonic-assisted ATPS can be a suitable method for extraction and enrichment of lignans from Z. armatum.
authors: School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. gt010010@sina.com.|Hainan Provincial Key Laboratory of R & D of Tropical Plants, Hainan Medical University, Haikou 571101, China. gt010010@sina.com.
authors: Tao
authors: T
authors: Guo
authors: School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. sudan58138@126.com.
authors: Dan
authors: D
authors: Su
authors: School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. hy123-0@163.com.
authors: Yan
authors: Y
authors: Huang
authors: School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. wangya502@163.com.
authors: Ya
authors: Y
authors: Wang
authors: Hainan Provincial Key Laboratory of R & D of Tropical Plants, Hainan Medical University, Haikou 571101, China. lyhssl@126.com.
authors: Yong-Hui
authors: YH
authors: Li
mesh_terms: D017705:Lignans / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D014465:Ultrasonics; D014867:Water / Q000737:chemistry*; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D017705:Lignans; D010936:Plant Extracts; D014867:Water
keywords: Zanthoxylum armatum; aqueous two-phase system; artificial neural network; lignans; response surface methodology; ultrasonic-assisted extraction