title: Long-term storage and preservation of tissue engineered articular cartilage.
abstract: With limited availability of osteochondral allografts, tissue engineered cartilage grafts may provide an alternative treatment for large cartilage defects. An effective storage protocol will be critical for translating this technology to clinical use. The purpose of this study was to evaluate the efficacy of the Missouri Osteochondral Allograft Preservation System (MOPS) for room temperature storage of mature tissue engineered grafts, focusing on tissue property maintenance during the current allograft storage window (28 days). Additional research compares MOPS to continued culture, investigates temperature influence, and examines longer-term storage. Articular cartilage constructs were cultured to maturity using adult canine chondrocytes, then preserved with MOPS at room temperature, in refrigeration, or kept in culture for an additional 56 days. MOPS storage maintained desired chondrocyte viability for 28 days of room temperature storage, retaining 75% of the maturity point Young's modulus without significant decline in biochemical content. Properties dropped past this time point. Refrigeration maintained properties similar to room temperature at 28 days, but proved better at 56 days. For engineered grafts, MOPS maintained the majority of tissue properties for the 28-day window without clearly extending that period as it had for native grafts. These results are the first evaluating engineered cartilage storage.
authors: Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, New York 10027.
authors: Adam B
authors: AB
authors: Nover
authors: Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, New York 10027.
authors: Robert M
authors: RM
authors: Stefani
authors: Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, New York 10027.
authors: Stephanie L
authors: SL
authors: Lee
authors: Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, New York 10027.|Department of Mechanical Engineering, Columbia University, 242 S. W. Mudd, 500 West 120th Street, Mail Code: 4703, New York, New York 10027.
authors: Gerard A
authors: GA
authors: Ateshian
authors: Department of Veterinary Medicine and Surgery, University of Missouri, 900 E Campus Dr., Columbia, Missouri 65211.
authors: Aaron M
authors: AM
authors: Stoker
authors: Department of Veterinary Medicine and Surgery, University of Missouri, 900 E Campus Dr., Columbia, Missouri 65211.|Department of Orthopaedic Surgery, University of Missouri, 1100 Virginia Avenue, DC953.000, Columbia, Missouri 65212.
authors: James L
authors: JL
authors: Cook
authors: Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, New York 10027.
authors: Clark T
authors: CT
authors: Hung
mesh_terms: D000818:Animals; D002358:Cartilage, Articular; D004285:Dogs; D011897:Random Allocation; D023822:Tissue Engineering; D014021:Tissue Preservation / Q000379:methods*
keywords: articular cartilage; preservation; storage; tissue engineering