title: UPLC-Q-TOF-MS(E) analysis of the constituents of Ding-Zhi-Xiao-Wan, a traditional Chinese antidepressant, in normal and depressive rats.
abstract: Ding-Zhi-Xiao-Wan (DZXW) is a traditional Chinese medicine widely used for treating depression. To clarify the bioactive constituents of DZXW, a new rapid ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS(E)) method was established in this study, with the whole extract of the formula separated into multiple components to facilitate the analytical process. In total, 97 compounds were detected and 88 were identified in DZXW. Based on their exact masses, fragmentation characteristics, and retention times, 85 of the 88 compounds were confirmed either conclusively or tentatively, and three potentially novel compounds were identified. In addition, following a three-day oral administration of DZXW, 60 and 28 compounds were observed in the plasma of normal and depressive rats, respectively. Finally, by combining our data with pharmacological information, 10 compounds were predicted as the likely bioactive constituents of DZXW as an antidepressant agent. Our approach provided a rapid method for characterising the chemical constituents of DZXW, and we were the first to screen for bioactive indexes in the plasma of depressive rats. Furthermore, our results provide useful chemical information that could be employed for further study of the pharmacodynamic material basis of DZXW's antidepressant effects.
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China; Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, PR China.
authors: Lu
authors: L
authors: Xu
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China.
authors: Li-Hua
authors: LH
authors: Mu
authors: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, PR China.
authors: Jie
authors: J
authors: Peng
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China; Tianjing University of Traditional Chinese Medicine, 88 Yuquan Road, Tianjin 300193, PR China.
authors: Wan-Wan
authors: WW
authors: Liu
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China.
authors: Xiao
authors: X
authors: Tan
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China; Tianjing University of Traditional Chinese Medicine, 88 Yuquan Road, Tianjin 300193, PR China.
authors: Zhao-Liang
authors: ZL
authors: Li
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China.
authors: Dong-Xiao
authors: DX
authors: Wang
authors: Department of Clinical Pharmacology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China. Electronic address: liuping301@126.com.
authors: Ping
authors: P
authors: Liu
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000032:analysis* / Q000627:therapeutic use; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D003863:Depression / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats
chemical_list: D000928:Antidepressive Agents; D004365:Drugs, Chinese Herbal; C000605449:Kai-Xin-San
keywords: CUMS; Chemical constituents; Depression; Ding-Zhi-Xiao-Wan; Metabolites; UPLC-Q-TOF-MS