title: Quencher-free molecular aptamer beacons (QF-MABs) for detection of ATP.
abstract: We have constructed a simple and efficient system-based on quencher-free molecular aptamer beacons (QF-MABs)-for probing ATP. In the absence of ATP, the fluorescence of a pyrene fluorophore on the loop position (15 nucleotides from the 5' end) of the optimal QF-MAB was quenched by the neighboring nucleobases; in its presence, fluorescence was recovered, due to a conformational change in the secondary structure of the QF-MAB.
authors: Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea. Electronic address: j.w.park@kisti.re.kr.
authors: Jung Woo
authors: JW
authors: Park
authors: Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors: Yoojin
authors: Y
authors: Park
authors: Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea. Electronic address: bhkim@postech.ac.kr.
authors: Byeang Hyean
authors: BH
authors: Kim
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011721:Pyrenes; D000255:Adenosine Triphosphate; C030984:pyrene
keywords: Aptamer; Aptamer molecular beacons; Fluorescence sensor; Pyrene; Quencher-free molecular beacon