title: Effect of Radix Sophorae Tonkinensis on the activity of cytochrome P450 isoforms in rats.
abstract: Radix Sophorae Tonkinensis (S. tonkinensis) is the processed lateral root of Sophora subprostrata (Leguminosae) that widely distributed over the southwest China. Radix Sophorae Tonkinensis has been widely used as a Chinese medicinal herb for the treatment of disease such as jaundice, inflammation, and aches. Herein, in order to investigate the effects of Radix Sophorae Tonkinensis on the metabolic capacity of rat cytochrome P450 (CYP) enzymes, we employed a cocktail method to evaluate the activities of CYP1A2, CYP2D6, CYP3A4, CYP2C19, CYP2C9 and CYP2B6. The experimental rats were randomly divided into two groups (control group and Radix Sophorae Tonkinensis treated group). The Radix Sophorae Tonkinensis treated group rats were given 5 g/kg Radix Sophorae Tonkinensis by continuous intragastric administration for 14 days. The mixture of six probes (phenacetin, metroprolol, midazolam, omeprazole, tolbutamide and bupropion) was given to rats by intragastric administration. The concentrations of probe drugs in rat plasma were measured by UPLC-MS/MS. The results showed that continuous intragastric administration for 14 days may inhibit the activities of rat CYP450 isoforms CYP2D6, CYP2C19 and CYP2B6. This finding may provide guidance for rational clinical uses of Radix Sophorae Tonkinensis.
authors: The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University Wenzhou 325000, China.
authors: Jinzhang
authors: J
authors: Cai
authors: Function Experiment Teaching Center of Wenzhou Medical University Wenzhou 325035, China.
authors: Jianshe
authors: J
authors: Ma
authors: Department of Medical Laboratory, Xiangya Medical School, Central South University Changsha 410013, China.
authors: Keqian
authors: K
authors: Xu
authors: Department of Medical Laboratory, Xiangya Medical School, Central South University Changsha 410013, China.
authors: Ge
authors: G
authors: Gao
authors: Maternal and Child Health Care Hospital of Hunan Province Changsha 410013, China.
authors: Yueyun
authors: Y
authors: Xiang
authors: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325035, China.
authors: Chongliang
authors: C
authors: Lin
keywords: CYP450; Radix Sophorae Tonkinensis; cocktail; rat