title: Enhanced immunogenicity of multivalent MUC1 glycopeptide antitumour vaccines based on hyperbranched polymers.
abstract: Enhancing the immunogenicity of an antitumour vaccine still poses a major challenge. It depends upon the selected antigen and the mode of its presentation. We here describe a fully synthetic antitumour vaccine, which addresses both aspects. For the antigen, a tumour-associated MUC1 glycopeptide as B-cell epitope was synthesised and linked to the immunostimulating T-cell epitope P2 derived from tetanus toxoid. The MUC1-P2 conjugate is presented multivalently on a hyperbranched polyglycerol to the immune system. In comparison to a related vaccine of lower multivalency, this vaccine exposing more antigen structures on the hyperbranched polymer induced significantly stronger immune responses in mice and elicited IgG antibodies of distinctly higher affinity to epithelial tumour cells.
authors: Johannes Gutenberg-University Mainz, Institute of Organic Chemistry, Duesbergweg 10-14, 55128 Mainz, Germany. hokunz@uni-mainz.de.
authors: M
authors: M
authors: Glaffig
authors: B
authors: B
authors: Palitzsch
authors: N
authors: N
authors: Stergiou
authors: C
authors: C
authors: Schüll
authors: D
authors: D
authors: Strassburger
authors: E
authors: E
authors: Schmitt
authors: H
authors: H
authors: Frey
authors: H
authors: H
authors: Kunz
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005990:Glycerol / Q000737:chemistry / Q000276:immunology*; D006020:Glycopeptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D018396:Mucin-1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011108:Polymers / Q000737:chemistry
chemical_list: D019496:Cancer Vaccines; D006020:Glycopeptides; D018396:Mucin-1; D011108:Polymers; C043941:polyglycerol; D005990:Glycerol