title: ZO-1 expression shows prognostic value in chronic B cell leukemia.
abstract: Connexin-mediated gap junctions are vital for tumor cell function. Intracellular pathways of connexin signaling use Zonula Occludens protein-1 (ZO-1) as an intermediate. This report describes the ZO-1 and connexin 43 (Cx43) expression pattern in lymphocytes from chronic B-cell leukemia (B-CLL) patients. The ZO-1 and Cx43 expression in the B cells of 113 B-CLL patients was identified. Western blot and flow cytometry were used to determine protein expression. Results indicated that ZO-1 and Cx43 expression was reduced and correlated negatively with CD38 and Zap-70 expression. Inhibition of intercellular communication with anti-Cx43 antibodies, 1-octanol, or carbenoxolone resulted in induced cell apoptosis. These data suggest that ZO-1, along with CD38 and Zap-70, plays a role in cell cycle regulation in B-CLL and may be used as a prognostic marker in B-CLL monitoring.
authors: Fundamental Medicine and Biology Department, Volgograd State Medical University, Volgograd, Russia; Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia. Electronic address: pavel.nesmiyanov@fulbrightmail.org.
authors: Pavel P
authors: PP
authors: Nesmiyanov
authors: Fundamental Medicine and Biology Department, Volgograd State Medical University, Volgograd, Russia; Department of Hematology, Volgograd Regional Clinical Oncology Dispensary No.1, Volgograd, Russia.
authors: Boris E
authors: BE
authors: Tolkachev
authors: Fundamental Medicine and Biology Department, Volgograd State Medical University, Volgograd, Russia; Volgograd Medical Science Center, Pharmacology Department, Laboratory for Genomics and Proteomics, Volgograd, Russia.
authors: Andrey V
authors: AV
authors: Strygin
mesh_terms: D020003:1-Octanol / Q000494:pharmacology; D051997:ADP-ribosyl Cyclase 1 / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002229:Carbenoxolone / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D018031:Connexin 43 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D061251:Primary Cell Culture; D011379:Prognosis; D015398:Signal Transduction; D016019:Survival Analysis; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000235:genetics / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D000906:Antibodies; D018031:Connexin 43; C500216:GJA1 protein, human; D008562:Membrane Glycoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D051746:ZAP-70 Protein-Tyrosine Kinase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1; D002229:Carbenoxolone; D020003:1-Octanol
keywords: B-cell chronic leukemia; Connexin43; Gap junction intercellular communication; Prognosis; ZO-1