title: Preparation and Characterization of RGDS/Nanodiamond as a Vector for VEGF-siRNA Delivery.
abstract: Small interfering RNA (siRNA) has great potential for treating or preventing diseases. Safe and efficient vectors are extremely needed for specific delivery of siRNA. Here VEGF-siRNA/RGDS/nanodiamond was prepared by conjugating Arg-Gly-Asp-Ser (RGDS) and VEGF-siRNA to nanodiamond delivery particles. VEGF-siRNA could be clinically used in anti-angiogenic gene therapy to inhibit tumor growth via the down-regulation of the expression of vascular endothelial growth factor (VEGF). The differential scanning calorimeter (DSC) analysis evidenced that RGDS and/or VEGF-siRNA were effectively linked to nanodiamond. The release assays indicated that in the presence of RGDS the release time of VEGF-siRNA was prolonged by 6 folds. This enabled VEGF-siRNA/RGDS/nanodiamond to release and transfer VEGF-siRNA in a long-acting manner, and thereby to significantly decrease the expression of VEGF mRNA and protein. Real-Time PCR analysis revealed that the expression of VEGF mRNA was decreased 87.56 ± 1.6% by VEGF-siRNA/RGDS/nanodiamond. Enzyme-Linked Immunosorbent Assay (ELISA) indicated that the expression of VEGF protein was down-regulated to 39.8 ± 1.8%. The down-regulation of VEGF protein expression was also observed in Western blotting experiments. In human umbilical vein endothelial cells (HUVEC) test, VEGF-siRNA/RGDS/nanodiamond decreased the formation of the tubes and exhibited no testable cytotoxicty. All the results consistently suggested that RGDS/nanodiamond is an ideal non-viral tumor-targeting vector for siRNA transfer, and VEGF-siRNA/RGDS/nanodiamond may be a promising regimen of gene therapy in carcinoma.
authors: Chunying
authors: C
authors: Cui
authors: Yuji
authors: Y
authors: Wang
authors: Kaikai
authors: K
authors: Yang
authors: Yaonan
authors: Y
authors: Wang
authors: Junyu
authors: J
authors: Yang
authors: Jianzhong
authors: J
authors: Xi
authors: Ming
authors: M
authors: Zhao
authors: Jianhui
authors: J
authors: Wu
authors: Shiqi
authors: S
authors: Peng
mesh_terms: D002470:Cell Survival / Q000235:genetics*; D003460:Crystallization / Q000379:methods; D020868:Gene Silencing / Q000502:physiology; D015316:Genetic Therapy / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D058612:Nanodiamonds / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry* / Q000493:pharmacokinetics; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D053769:Nanocapsules; D058612:Nanodiamonds; D009842:Oligopeptides; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; C046617:arginyl-glycyl-aspartyl-serine