title: Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata.
abstract: During evolution of animals, their co-evolution with bacteria has generally been ignored. Recent studies have provided evidences that the symbiotic bacteria in the animal gut can either be essential or contributing to the plasticity of the host. The Crustacea includes crab, crayfish, lobster, and shrimp and represents the second largest subphylum on the planet. Although there are already studies investigating the intestinal bacterial communities in crustaceans, none of them has examined the microbiota in different parts of the digestive system during the gonad development of the host. Here, we utilized a new shrimp model Neocaridina denticulata and sequenced the 16S rRNA using the Ion Torrent platform to survey the bacterial populations colonizing the hepatopancreas, foregut, and intestine, including midgut and hindgut, of the early, mid, and late ovarian maturation stages of the shrimp. The predominant bacteria phylum was found to be Proteobacteria, with more than 80 % reads from the gut flora at the early gonad development belonged to a Coxiella-type bacterium. Distinct bacterial communities can be detected between the hepatopancreas and gut, although no significant difference could be revealed between the different regions of the gut investigated. Surprisingly, during the gonad development, bacterial diversity changed rapidly in the gut but not the hepatopancreas. This study provides the first evidence that microbiota modified differentially in specific regions of the digestive tract during gonadal development of crustaceans.
authors: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
authors: Man Kit
authors: MK
authors: Cheung
authors: Simon F.S. Li Marine Science Laboratory, School of Life Sciences, Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
authors: Ho Yin
authors: HY
authors: Yip
authors: Simon F.S. Li Marine Science Laboratory, School of Life Sciences, Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
authors: Wenyan
authors: W
authors: Nong
authors: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.|The Nethersole School of Nursing, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
authors: Patrick Tik Wan
authors: PT
authors: Law
authors: Simon F.S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
authors: Ka Hou
authors: KH
authors: Chu
authors: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
authors: Hoi Shan
authors: HS
authors: Kwan
authors: Simon F.S. Li Marine Science Laboratory, School of Life Sciences, Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR. jeromehui@cuhk.edu.hk.
authors: Jerome Ho Lam
authors: JH
authors: Hui
mesh_terms: D000818:Animals; D044822:Biodiversity; D033364:Decapoda / Q000254:growth & development / Q000382:microbiology*; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D006066:Gonads / Q000254:growth & development*; D043143:Hepatopancreas / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction
keywords: Crustacean; Evolution; Gonad development; Gut; Hepatopancreas; Ion Torrent; Microbiota; Shrimp