title: Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
abstract: This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Ana Patrícia C
authors: AP
authors: Silva
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Auricélio A
authors: AA
authors: Macêdo
authors: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Luciana F
authors: LF
authors: Costa
authors: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Cláudia E
authors: CE
authors: Rocha
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Luize N N
authors: LN
authors: Garcia
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Jade R D
authors: JR
authors: Farias
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Priscilla P R
authors: PP
authors: Gomes
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Gustavo C
authors: GC
authors: Teixeira
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Kessler W J
authors: KW
authors: Fonseca
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Andréa R F
authors: AR
authors: Maia
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Gabriela G
authors: GG
authors: Neves
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Everton L
authors: EL
authors: Romão
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Teane M A
authors: TM
authors: Silva
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Juliana P S
authors: JP
authors: Mol
authors: Departamento de Patologia, Universidade Estadual do Maranhão, 65057-630 São Luís, MA, Brazil.
authors: Renata M
authors: RM
authors: Oliveira
authors: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, MG, Brazil.
authors: Márcio S S
authors: MS
authors: Araújo
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Ernane F
authors: EF
authors: Nascimento
authors: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, MG, Brazil.
authors: Olindo A
authors: OA
authors: Martins-Filho
authors: Embrapa Gado de Leite, 36038-330 Juiz de Fora, MG, Brazil.
authors: Humberto M
authors: HM
authors: Brandão
authors: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Tatiane A
authors: TA
authors: Paixão
authors: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors: Renato L
authors: RL
authors: Santos
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000172:deficiency*; D000464:Alginates / Q000737:chemistry; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology; D002004:Brucella Vaccine / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D042061:Brucella ovis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D002214:Capsules / Q000008:administration & dosage / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007279:Injections, Subcutaneous; D008297:Male; D012661:Semen / Q000382:microbiology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D034561:Sheep, Domestic; D014556:Urine / Q000382:microbiology
chemical_list: D018528:ATP-Binding Cassette Transporters; D000464:Alginates; D001426:Bacterial Proteins; D002004:Brucella Vaccine; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid