title: MicroRNA-148a inhibits migration of breast cancer cells by targeting MMP-13.
abstract: Breast cancer is a threat to the health of women, and metastasis of breast cancer cells plays an important role in the deterioration of breast cancer. MicroRNAs play a critical role in the tumorigenesis and development of breast cancer. MicroRNA-148a (miR-148a) is associated with the growth and metastasis of tumor cells. In the present study, we investigated the role of miR-148a in migration of breast cancer cells as well as the underlying mechanism. MiR-148a was found to inhibit the proliferation and migration of breast cancer cells. To further explore the mechanism through which miR-148a plays its antitumor role, matrix metalloproteinase-13 (MMP-13) was identified as a target of miR-148a by western blot and luciferase reporter assay. Moreover, silence of MMP-13 mimicked the effect of miR-148a, whereas overexpression of MMP-13 rescued the impaired migration caused by miR-148a. Our study demonstrates that miR-148a inhibits the migration of breast cancer cells by targeting MMP-13 and also lays theoretical foundation for further exploration for the function of miR-148a.
authors: The Seventh Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
authors: Jinqi
authors: J
authors: Xue
authors: Department of Spine and Joint Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China.
authors: Zhiguang
authors: Z
authors: Chen
authors: The Seventh Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
authors: Xi
authors: X
authors: Gu
authors: The Seventh Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
authors: Yang
authors: Y
authors: Zhang
authors: The Seventh Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China. zhangwh_sjhospital@163.com.
authors: Wenhai
authors: W
authors: Zhang
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis* / Q000235:genetics; D035683:MicroRNAs / Q000502:physiology*; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: C550570:MIRN148 microRNA, human; D035683:MicroRNAs; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13
keywords: Breast cancer cells; MMP-13; MiR-148a; Migration