title: Single cell nanobiosensors for dynamic gene expression profiling in native tissue microenvironments.
abstract: A gold nanorod-locked nucleic acid nano-biosensor for dynamic single-cell gene expression analysis in living cells and tissues is developed. The nanoparticle facilitates endocytic delivery and dynamic monitoring of the gene expression in human umbilical cord endothelial cells, mouse skin tissues, mouse retina tissues, and mouse cornea tissues at the single-cell level.
authors: Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
authors: Shue
authors: S
authors: Wang
authors: Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology and Harvard Medical School, Cambridge, MA, 02139, USA.
authors: Reza
authors: R
authors: Riahi
authors: Department of Mechanical and Aerospace Engineering, University of Miami, Coral Gables, FL, 33146, USA.
authors: Na
authors: N
authors: Li
authors: Department of Pharmacology and Toxicology, University of Arizona, Tucson, AZ, 85724, USA.
authors: Donna D
authors: DD
authors: Zhang
authors: Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.|Department of Biomedical Engineering and Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
authors: Pak Kin
authors: PK
authors: Wong
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D008810:Mice, Inbred C57BL; D043942:Nanotubes / Q000737:chemistry; D015341:Nucleic Acid Probes / Q000737:chemistry; D010316:Particle Size; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D015341:Nucleic Acid Probes; D012333:RNA, Messenger; D006046:Gold
keywords: biosensors; locked nucleic acids; nanoparticles; single cells; tissue microenvironments