title: Application of isotope labeling experiments and (13)C flux analysis to enable rational pathway engineering.
abstract: Isotope labeling experiments (ILEs) and (13)C flux analysis provide actionable information for metabolic engineers to identify knockout, overexpression, and/or media optimization targets. ILEs have been used in both academic and industrial labs to increase product formation, discover novel metabolic functions in previously uncharacterized organisms, and enhance the metabolic efficiency of host cell factories. This review highlights specific examples of how ILEs have been used in conjunction with enzyme or metabolic engineering to elucidate host cell metabolism and improve product titer, rate, or yield in a directed manner. We discuss recent progress and future opportunities involving the use of ILEs and (13)C flux analysis to characterize non-model host organisms and to identify and subsequently eliminate wasteful byproduct pathways or metabolic bottlenecks.
authors: Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA.
authors: Allison G
authors: AG
authors: McAtee
authors: Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA.
authors: Lara J
authors: LJ
authors: Jazmin
authors: Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University, PMB 351604, Nashville, TN 37235-1604, USA. Electronic address: j.d.young@vanderbilt.edu.
authors: Jamey D
authors: JD
authors: Young
mesh_terms: D057005:Bioengineering / Q000379:methods*; D002247:Carbon Isotopes / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008954:Models, Biological
chemical_list: D002247:Carbon Isotopes