title: Effect of sodium acetate on the adhesion to porcine gastric mucin in a Lactococcus lactis strain grown on fructose.
abstract: The association of lactic acid bacteria with mucosal surfaces plays important roles in the beneficial effects of these bacteria on human health, such as colonization of the gastrointestinal tract for pathogen antagonism. Previously, we found that the adhesion of Lactococcus lactis 7-1 to porcine gastric mucin was higher with fructose than with lactose, galactose or xylose as the carbon source. In this study, we examined the effect of growth conditions on the adhesion of strain 7-1 grown on fructose. Medium components affect the adhesion: the adhesion of strain 7-1 grown with sodium acetate was higher than that without it. The enhancement of adhesion by sodium acetate was not observed under aerobic conditions. Cellular properties grown with or without sodium acetate were characterized: strain 7-1 grown with sodium acetate had similar sugar contents, and different fatty acid composition to those grown without it. Strain 7-1 grown with sodium acetate showed significantly lower cell yield and significantly higher hydrophobicity than those grown without it, which is associated with higher adhesion. Fructose and sodium acetate are frequently used in the food industry; this study may reveal a simple way to enhance the adhesion of lactic acid bacteria by growing them with these substances.
authors: NARO Institute of Livestock and Grassland Science, Tsukuba.
authors: Hiromi
authors: H
authors: Kimoto-Nira
authors: NARO Institute of Livestock and Grassland Science, Tsukuba.
authors: Naoko
authors: N
authors: Moriya
authors: Japan International Research Center for Agricultural Sciences, Tsukuba.
authors: Seishi
authors: S
authors: Yamasaki
authors: National Institute of Agrobiological Sciences, Tsukuba, Japan.
authors: Akio
authors: A
authors: Takenaka
authors: NARO Institute of Livestock and Grassland Science, Tsukuba.
authors: Chise
authors: C
authors: Suzuki
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005227:Fatty Acids / Q000378:metabolism; D005632:Fructose / Q000494:pharmacology; D005752:Gastric Mucins / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D013294:Lactococcus lactis / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D019936:Probiotics; D019346:Sodium Acetate / Q000494:pharmacology*; D013552:Swine
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D005752:Gastric Mucins; D005632:Fructose; D019346:Sodium Acetate
keywords: adhesive activity; fructose; probiotics; sodium acetate