title: Molecular Inflammation in the Contralateral Eye After Cataract Surgery in the First Eye.
abstract: OBJECTIVE
  The purpose of this study was to assess the inflammatory status of the aqueous humor in the fellow eye after uneventful cataract surgery in the first eye.
  
  
  METHODS
  At the screening stage, aqueous humor samples from 15 first-eye and 15 second-eye cataract patients were collected just before cataract surgery and assayed using human cytokine antibody array. Screened cytokines were then verified using a suspension array system with aqueous humor samples obtained from 35 first-eye and 36 second-eye cataract patients.
  
  
  RESULTS
  The cytokine antibody array revealed that interleukin-1 receptor antagonist (Il-1ra) and macrophage inflammatory protein (MIP)-1a and MIP-1b were expressed at high levels in first-eye patients and were lower in second-eye patients, whereas opposite trends were found for monocyte chemoattractant protein 1 (MCP-1) and for regulated on activation, normal T expressed and secreted (RANTES) (all, P < 0.05, Student's t-test). However, only MCP-1 and IL-1ra were significantly different between the two groups after Bonferroni correction (both P < 0.00125). In the replication stage, the suspension cytokine array revealed that only MCP-1 expression was significantly greater in the aqueous humor of second-eye patients than in that of first-eye patients (P = 0.0067, Student's t-test).
  
  
  CONCLUSIONS
  This study revealed that expression of MCP-1, a pain-related inflammatory chemokine, was significantly increased in aqueous humor in the contralateral eye after first-eye cataract surgery. This suggests there may be a sympathetic ophthalmic type uveitis in the contralateral eye after first-eye cataract surgery and that may help to explain why second-eye phacoemulsification is often more painful. (ClinicalTrials.gov number, NCT01824927.)
authors: Department of Ophthalmology Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People's Republic of China; Key Laboratory of Myopia, Ministry of Health, People's Republic of China; and Shanghai Key Laboratory of Visual Impairment and Resto.
authors: Xiang-Jia
authors: XJ
authors: Zhu
authors: Parkway Health Hongqiao Medical Center, Shanghai, People's Republic of China.
authors: Don
authors: D
authors: Wolff
authors: Department of Ophthalmology Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People's Republic of China; Key Laboratory of Myopia, Ministry of Health, People's Republic of China; and Shanghai Key Laboratory of Visual Impairment and Resto.
authors: Ke-Ke
authors: KK
authors: Zhang
authors: Department of Ophthalmology Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People's Republic of China; Key Laboratory of Myopia, Ministry of Health, People's Republic of China; and Shanghai Key Laboratory of Visual Impairment and Resto.
authors: Wen-Wen
authors: WW
authors: He
authors: Department of Ophthalmology Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People's Republic of China; Key Laboratory of Myopia, Ministry of Health, People's Republic of China; and Shanghai Key Laboratory of Visual Impairment and Resto.
authors: Xing-Huai
authors: XH
authors: Sun
authors: Department of Ophthalmology Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People's Republic of China; Key Laboratory of Myopia, Ministry of Health, People's Republic of China; and Shanghai Key Laboratory of Visual Impairment and Resto.
authors: Yi
authors: Y
authors: Lu
authors: Parkway Health Hongqiao Medical Center, Shanghai, People's Republic of China.
authors: Peng
authors: P
authors: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D002386:Cataract / Q000378:metabolism*; D002387:Cataract Extraction; D016207:Cytokines / Q000032:analysis*; D005123:Eye / Q000378:metabolism*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007908:Lens, Crystalline / Q000737:chemistry; D008297:Male; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D016207:Cytokines