title: Pyrosequencing analysis of bacterial communities in biofilms from different pipe materials in a city drinking water distribution system of East China.
abstract: Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe (DCIP), gray cast iron pipe (GCIP), galvanized steel pipe (GSP), stainless steel clad pipe (SSCP), and polyvinyl chloride (PVC), were collected from an actual DWDS to investigate the effect of pipe material on bacterial community. Real-time quantitative polymerase chain reaction (qPCR) and culture-based method were used to quantify bacteria. 454 pyrosequencing was used for bacterial community analysis. The results showed that the numbers of total bacteria and culturable heterotrophic bacteria from iron pipes were higher than that in PVC, while the numbers of Shigella and vibrios were low in biofilms from iron pipes. Bacterial community analysis showed that Hyphomicrobium or Desulfovibrio were the predominant microorganism in iron pipes, whereas Sphingomonas or Pseudomonas were dominant in other types of pipe. This study revealed differences in bacterial communities in biofilms among different pipe materials, and the results were useful for pipeline material selection in DWDSs.
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors: Hongxing
authors: H
authors: Ren
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors: Wei
authors: W
authors: Wang
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors: Yan
authors: Y
authors: Liu
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors: Shuai
authors: S
authors: Liu
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors: Liping
authors: L
authors: Lou
authors: Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, 310053, China.
authors: Dongqing
authors: D
authors: Cheng
authors: Shaoxing Water Environmental Science Institute Co. Ltd, Shaoxing, 312000, China.
authors: Xiaofang
authors: X
authors: He
authors: Shaoxing Water Environmental Science Institute Co. Ltd, Shaoxing, 312000, China.
authors: Xiaoyan
authors: X
authors: Zhou
authors: Shaoxing Water Environmental Science Institute Co. Ltd, Shaoxing, 312000, China.
authors: Shangde
authors: S
authors: Qiu
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors: Liusong
authors: L
authors: Fu
authors: College of Civil Engineering and Architecture, Zhejiang University, Hangzhou, 310058, China. liujingqing@zju.edu.cn.
authors: Jingqing
authors: J
authors: Liu
authors: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China. blhu@zju.edu.cn.
authors: Baolan
authors: B
authors: Hu
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D058448:Biota; D002681:China; D002947:Cities; D060766:Drinking Water; D004783:Environmental Microbiology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D060766:Drinking Water
keywords: Bacterial community; Biofilms; Drinking water distribution system; Pipe materials