title: Decaffeinated green tea extract rich in epigallocatechin-3-gallate prevents fatty liver disease by increased activities of mitochondrial respiratory chain complexes in diet-induced obesity mice.
abstract: Nonalcoholic fatty liver disease has been considered the hepatic manifestation of obesity. It is unclear whether supplementation with green tea extract rich in epigallocatechin-3-gallate (EGCG) influences the activity of mitochondrial respiratory chain complexes and insulin resistance in the liver. EGCG regulated hepatic mitochondrial respiratory chain complexes and was capable of improving lipid metabolism, attenuating insulin resistance in obese mice. Mice were divided into four groups: control diet+water (CW) or EGCG (CE) and hyperlipidic diet+water (HFW) or EGCG (HFE). All animals received water and diets ad libitum for 16 weeks. Placebo groups received water (0.1 ml/day) and EGCG groups (0.1 ml EGCG and 50 mg/kg/day) by gavage. Cytokines concentrations were obtained by ELISA, protein expression through Western blotting and mitochondrial complex enzymatic activity by colorimetric assay of substrate degradation. HFW increased body weight gain, adiposity index, retroperitoneal and mesenteric adipose tissue relative weight, serum glucose, insulin and Homeostasis Model Assessment of Basal Insulin Resistance (HOMA-IR); glucose intolerance was observed in oral glucose tolerance test (OGTT) as well as ectopic fat liver deposition. HFE group decreased body weight gain, retroperitoneal and mesenteric adipose tissue relative weight, HOMA-IR, insulin levels and liver fat accumulation; increased complexes II-III and IV and malate dehydrogenase activities and improvement in glucose uptake in OGTT and insulin sensitivity by increased protein expression of total AKT, IRα and IRS1. We did not find alterations in inflammatory parameters analyzed. EGCG was able to prevent obesity stimulating the mitochondrial complex chain, increasing energy expenditure, particularly from the oxidation of lipid substrates, thereby contributing to the prevention of hepatic steatosis and improved insulin sensitivity.
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil.
authors: Aline B
authors: AB
authors: Santamarina
authors: Programa de Pós-Graduação em Ciência da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors: Milena
authors: M
authors: Carvalho-Silva
authors: Programa de Pós-Graduação em Ciência da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors: Lara M
authors: LM
authors: Gomes
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil.
authors: Marcos H
authors: MH
authors: Okuda
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil.
authors: Aline A
authors: AA
authors: Santana
authors: Programa de Pós-Graduação em Ciência da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors: Emilio L
authors: EL
authors: Streck
authors: Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil.
authors: Marilia
authors: M
authors: Seelaender
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil.
authors: Claudia M Oller
authors: CM
authors: do Nascimento
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil.
authors: Eliane B
authors: EB
authors: Ribeiro
authors: Exercise and Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, Presidente Prudente, SP 19060-900, Brazil.
authors: Fábio S
authors: FS
authors: Lira
authors: Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP 04021-001, Brazil. Electronic address: lmoyama@gmail.com.
authors: Lila Missae
authors: LM
authors: Oyama
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D004798:Enzymes / Q000378:metabolism; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000517:prevention & control*; D009765:Obesity / Q000150:complications* / Q000178:diet therapy / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D016207:Cytokines; D004798:Enzymes; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate
keywords: EGCG; Insulin resistance; Mice; NAFLD; Obesity; Respiratory chain