title: F-actin localization dynamics during appressorium formation in Colletotrichum graminicola.
abstract: Appressoria are essential penetration structures for many phytopathogenic fungi. Here F-actin localization dynamics were documented during appressorium formation in vitro and in planta in Colletotrichum graminicola Four discernible stages of dynamic F-actin distribution occurring in a programmed order were documented from differentiation of appressoria to formation of penetration pores: (stage A) from germ tube enlargement to complete expansion of the appressorium; (stage S) septation occurs; (stage L) a long period of low F-actin activity; (stage P) the penetration pore forms. The F-actin subcellular localization corresponded to each stage. A distinct redistribution of actin cables occurred at the transition from stage A to stage S. The in planta assays revealed that F-actin also assembled in invasive hyphae and that actin cables might play an essential role for penetration-peg development. The F-actin localization distribution may be used as a subcellular marker to define the developmental stages during appressorium formation.
authors: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, and Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan.
authors: Chih-Li
authors: CL
authors: Wang
authors: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas bdshaw@tamu.edu.
authors: Brian D
authors: BD
authors: Shaw
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D020231:Colletotrichum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D021381:Protein Transport; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000199:Actins; D005656:Fungal Proteins
keywords: Lifeact; actin; anthracnose; appressorium; maize; penetration peg