title: Impacts of BDE209 addition on Pb uptake, subcellular partitioning and gene toxicity in earthworm (Eisenia fetida).
abstract: Lead (Pb) and decabromodiphenyl ether (BDE209) are the mainly co-existed contaminants at e-waste recycling sites. The potential toxicity of Pb (250 μg g(-1)) to earthworm Eisenia fetida in the presence of BDE209 (1, 10 and 100 μg g(-1)) was determined during 14-d incubation period. Compared to Pb treatment alone, the co-exposure with 1 μg g(-1) BDE209 barely affected Pb uptake, subcellular partitioning and gene expression; however, histopathological changes in earthworms' body wall (epidermal, circular and longitudinal muscles) demonstrated that 10 and 100 μg g(-1) BDE209 additions enhanced Pb uptake and altered its subcellular partitioning, indicating that Pb redistributed from fractions E (cell debris) and D (metal-rich granules) to fraction C (cytosols); Additionally, BDE209 supply significantly inhibited (p<0.05) the induction of SOD (superoxide dismutase) and CAT (catalase) gene expressions (maximum down-regulation 59% for SOD gene at Pb+100 μg g(-1) BDE209 and 89% for CAT gene at Pb+10 μg g(-1) BDE209), while facilitated (p<0.05) Hsp90 (heat shock protein 90) gene expression with maximum induction rate of 120% after exposure to Pb+10 μg g(-1) BDE209. These findings illustrate the importance of considering environmental BDE209 co-exposure when assessing Pb bioaccumulation and toxicity in multi-contaminated soil ecosystems.
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wzhang@ecust.edu.cn.
authors: Wei
authors: W
authors: Zhang
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Kou
authors: K
authors: Liu
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Jing
authors: J
authors: Li
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Jun
authors: J
authors: Liang
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Kuangfei
authors: K
authors: Lin
mesh_terms: D000818:Animals; D002374:Catalase / Q000096:biosynthesis; D003600:Cytosol / Q000378:metabolism; D004581:Electronics; D015870:Gene Expression / Q000187:drug effects; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000494:pharmacology*; D007220:Industrial Waste; D007854:Lead / Q000378:metabolism* / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D009835:Oligochaeta / Q000187:drug effects / Q000235:genetics*; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity*; D013347:Subcellular Fractions / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis
chemical_list: D018841:HSP90 Heat-Shock Proteins; D055768:Halogenated Diphenyl Ethers; D007220:Industrial Waste; D009153:Mutagens; D012989:Soil Pollutants; D007854:Lead; D002374:Catalase; D013482:Superoxide Dismutase; C010902:decabromobiphenyl ether
keywords: BDE209; Eisenia fetida; Gene transcription; Pb; Subcellular partitioning