title: High throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery.
abstract: OBJECTIVE
  To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines.
  
  
  METHODS
  The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits.
  
  
  RESULTS
  The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested from patients underwent NAC showed significant higher profiles of interleukin-1β (IL-1β), IL-4, IL-6, IL-17F, IL-21, IL-23, IL-25, IL-31, Interferon γ (IFNγ), CD40 ligand (CD40L), tumor necrosis factor α (TNFα), CXCL1, CXCL2, CXCL5, CCL3, CCL7 and CCL20.
  
  
  CONCLUSIONS
  Surgery-induced WF promotes the proliferation and migration of breast cancer cells. The composition of WF is influenced by various clinical features and provides potential therapeutic targets to control local recurrence and tumor progression.
authors: Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023.
authors: Dan
authors: D
authors: Wang
authors: Department of Urology, First Hospital of Jilin University, Changchun, China 130021.
authors: Kebang
authors: K
authors: Hu
authors: Ultrasonic Diagnosis Department, The First Hospital of China Medical University, Shenyang, China 110001.
authors: Ningning
authors: N
authors: Gao
authors: Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023.
authors: Hao
authors: H
authors: Zhang
authors: Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023.
authors: Yanlin
authors: Y
authors: Jiang
authors: Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023.
authors: Caigang
authors: C
authors: Liu
authors: Department of Surgery, The First Hospital of Dalian Medical University, Dalian, China 114000.
authors: Shouyu
authors: S
authors: Wang
authors: Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023.
authors: Zuowei
authors: Z
authors: Zhao
mesh_terms: D001826:Body Fluids / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery*; D002465:Cell Movement; D049109:Cell Proliferation; D018925:Chemokines / Q000378:metabolism*; D017024:Chemotherapy, Adjuvant; D016207:Cytokines / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D057166:High-Throughput Screening Assays; D006801:Humans; D061986:MCF-7 Cells; D008408:Mastectomy / Q000009:adverse effects*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D015398:Signal Transduction; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D018925:Chemokines; D016207:Cytokines; D020782:Matrix Metalloproteinases
keywords: breast cancer; proliferation; wound fluid