title: Rapid assay of A2058T-mutated 23S rRNA allelic profiles associated with high-level macrolide resistance in Moraxella catarrhalis.
abstract: We report on a restriction fragment-length polymorphism (HpyCH4III) assay for profile analysis of 23S rRNA gene A2058T-mutated alleles associated with high-level macrolide resistance in Moraxella catarrhalis. Our assay results were supported by DNA sequencing analysis, allowed for simultaneous testing of many strains, and produced results from pure-cultured colonies within 4 h.
authors: Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Ryoichi
authors: R
authors: Saito
authors: Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Ayako
authors: A
authors: Kasai
authors: Department of Clinical Laboratory, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
authors: Shinji
authors: S
authors: Ogihara
authors: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Corporation Toshima Hospital, Tokyo, Japan.
authors: Kageto
authors: K
authors: Yamada
authors: Radioisotope Center, University of Tokyo, Tokyo, Japan.
authors: Kazuyuki
authors: K
authors: Tao
mesh_terms: D000900:Anti-Bacterial Agents / Q000145:classification / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D018942:Macrolides / Q000494:pharmacology*; D001936:Moraxella catarrhalis / Q000187:drug effects* / Q000235:genetics; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S