title: Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process.
abstract: To explore the metabolic versatility of "Candidatus Brocadia sinica" in the presence of VFAs, the impacts of VFAs on anammox activity and nitrogen removal were investigated in this study. Results found that low VFAs concentrations has no affect on anammox activity and the removal efficiencies of NH4(+)-N and NO2(-)-N. However, "Ca. Brocadia sinica" showed higher adaptability to some VFAs stresses. Furthermore, Illumina MiSeq pyrosequencing results indicated that the microbial community structures varied significantly and the phyla Chloroflexi, Proteobacteria, Bacteroidetes and Chlorobi were dominated. Finally, qPCR was performed to validate the growth of anammox bacteria and gain the quantitative insights into the correlation between nitrogen transformation rates and the key functional genes in the organotrophic anammox system. Combined analysis clearly demonstrated that the coupling of the anammox, denitrification and DNRA were the noteworthy pathway for the simultaneous removal of nitrogen and organic carbon.
authors: Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Shaanxi 710049, China.
authors: Duntao
authors: D
authors: Shu
authors: School of Human Settlements & Civil Engineering, Xi'an Jiaotong University, Shaanxi 710049, China. Electronic address: heyl@mail.xjtu.edu.cn.
authors: Yanling
authors: Y
authors: He
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Hong
authors: H
authors: Yue
authors: Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China.
authors: Liang
authors: L
authors: Zhu
authors: School of Chemical Engineering & Technology, Xi'an Jiaotong University, Shaanxi 710049, China.
authors: Qingyi
authors: Q
authors: Wang
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000737:chemistry / Q000378:metabolism; D054892:Metagenome / Q000235:genetics*; D056186:Metagenomics; D059013:Microbial Consortia / Q000235:genetics*; D009566:Nitrates / Q000378:metabolism*
chemical_list: D005232:Fatty Acids, Volatile; D009566:Nitrates; D000641:Ammonia
keywords: Biological nitrogen removal; Candidatus Brocadia sinica; MiSeq pyrosequencing; Microbial community; Organotrophic anammox