title: A Generalizable Platform for the Photoactivation of Cell Surface Receptors.
abstract: Polarized signal transduction from cell surface receptors plays a central role in the development and homeostasis of multicellular organisms, and it also contributes to cellular dysfunction in many disease states. Understanding the molecular and cellular bases of polarized signaling requires experimental methods that provide precise spatiotemporal control of receptor activation. However, we currently lack strategies for inducing both sustained and spatially constrained signal transduction. In the present study, we combined synthetic and cell biological tools to develop a generalizable photoactivation approach for the stimulation of cell surface receptors. Our system, which is based upon the local decaging of a "universal" peptide ligand, is particularly well suited for the live imaging of single cells. We anticipate that it will greatly facilitate future mechanistic analyses of polarized signal transduction in a variety of cell types.
authors: Tri-Institutional Program in Chemical Biology, Weill Cornell Medical College , 1300 York Avenue, New York, New York 10065, United States.
authors: Thinh Nguyen
authors: TN
authors: Duc
authors: Immunology Program, Memorial Sloan-Kettering Cancer Center , 1275 York Avenue, New York, New York 10065, United States.
authors: Morgan
authors: M
authors: Huse
mesh_terms: D006801:Humans; D008027:Light; D055668:Photochemical Processes; D010777:Photochemistry / Q000295:instrumentation*; D011956:Receptors, Cell Surface / Q000502:physiology* / Q000528:radiation effects; D015398:Signal Transduction
chemical_list: D011956:Receptors, Cell Surface