title: Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation.
abstract: The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision- with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.
authors: Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, NSW, 2522, Australia. School of Health Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia. toddm@uow.edu.au.
authors: Todd
authors: T
authors: Mitchell
authors: School of Chemistry, University of Wollongong, Wollongong, NSW, 2522, Australia. Central Analytical Research Facility, Queensland University of Technology, Brisbane QLD, 4001, Australia. stephen.blanksby@qut.edu.au.
authors: Stephen
authors: S
authors: Blanksby
authors: School of Chemistry, University of Wollongong, Wollongong, NSW, 2522, Australia. Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, NSW, 2522, Australia. rk843@uow.edu.au.
authors: Rachel
authors: R
authors: Kozlowskia
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D010126:Ozone / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D010713:Phosphatidylcholines; D010126:Ozone