title: Undibacterium aquatile sp. nov., isolated from a waterfall.
abstract: A Gram-stain-negative, aerobic, motile and rod-shaped strain, THG-DN7.3T, was isolated from a waterfall. Strain THG-DN7.3T grew well at 18-28 °C and at pH 6.0-7.5 on Reasoner's 2A agar. Based on 16S rRNA gene sequence comparisons, strain THG-DN7.3T was most closely related to Undibacterium jejuense JS4-4T (97.3 % 16S rRNA gene sequence similarity) and Undibacterium seohonense SHS5-24T (96.5 %). The G+C content of the genomic DNA was 57.4 mol%. The mean DNA-DNA relatedness of strain THG-DN7.3T with U. jejuense KACC 12607T was 40 ± 1 % (reciprocal 50 ± 2.1 %). The major cellular fatty acids of strain THG-DN7.3T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (47.4 %), C16 : 0 (30.4 %), summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) (6.8 %) and C12 : 0 (6.2 %). The predominant isoprenoid quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of the DNA-DNA hybridization and genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-DN7.3T represents a novel species of the genus Undibacterium, for which the name Undibacterium aquatile sp. nov. is proposed. The type strain is THG-DN7.3T ( = KCTC 42243T = CCTCC AB 2015119T).
authors: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446-701, Republic of Korea.
authors: Juan
authors: J
authors: Du
authors: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446-701, Republic of Korea.
authors: Shahina
authors: S
authors: Akter
authors: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446-701, Republic of Korea.
authors: KyungHwa
authors: K
authors: Won
authors: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446-701, Republic of Korea.
authors: Hina
authors: H
authors: Singh
authors: Department of Acupuncture Merdian Science Research Center, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors: Chang
authors: C
authors: Shik Yin
authors: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors: MooChang
authors: M
authors: Kook
authors: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446-701, Republic of Korea.
authors: Tae-Hoo
authors: TH
authors: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8