title: Iron oxides in human spleen.
abstract: Iron is an essential element for fundamental cell functions and a catalyst for chemical reactions. Three samples extracted from the human spleen were investigated by scanning (SEM) and transmission electron microscopy (TEM), Mössbauer spectrometry (MS), and SQUID magnetometry. The sample with diagnosis of hemosiderosis (H) differs from that referring to hereditary spherocytosis and the reference sample. SEM reveals iron-rich micrometer-sized aggregate of various structures-tiny fibrils in hereditary spherocytosis sample and no fibrils in hemochromatosis. Hematite and magnetite particles from 2 to 6 μm in TEM with diffraction in all samples were shown. The SQUID magnetometry shows different amount of diamagnetic, paramagnetic and ferrimagnetic structures in the tissues. The MS results indicate contribution of ferromagnetically split sextets for all investigated samples. Their occurrence indicates that at least part of the sample is magnetically ordered below the critical temperature. The iron accumulation process is different in hereditary spherocytosis and hemosiderosis. This fact may be the reason of different iron crystallization.
authors: Department of Medical Physics, Biophysics, Informatics and Telemedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia. martin.kopani@fmed.uniba.sk.
authors: Martin
authors: M
authors: Kopáni
authors: Institute of Nuclear and Physical Engineering, Slovak University of Technology, Bratislava, Slovakia.|Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic.
authors: Marcel
authors: M
authors: Miglierini
authors: Institute of Inorganic Chemistry, v.v.i. AV CR, Husinec-Rez, Czech Republic.
authors: Adriana
authors: A
authors: Lančok
authors: Institute of Nuclear and Physical Engineering, Slovak University of Technology, Bratislava, Slovakia.
authors: Július
authors: J
authors: Dekan
authors: Department of Geology of Mineral Deposits, Faculty of Natural Science, Comenius University, Bratislava, Slovakia.|STU Center for Nanodiagnosis, Slovak University of Technology, Bratislava, Slovakia.
authors: Mária
authors: M
authors: Čaplovicová
authors: Institute of Histology and Embryology, Comenius University, Bratislava, Slovakia.
authors: Ján
authors: J
authors: Jakubovský
authors: Department of Chemistry, FPV, University of SS Cyril and Methodius, Trnava, Slovakia.
authors: Roman
authors: R
authors: Boča
authors: Department of Pathology, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors: Hedviga
authors: H
authors: Mrazova
mesh_terms: D001344:Autopsy; D003460:Crystallization; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006486:Hemosiderosis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D015204:Spectroscopy, Mossbauer; D013103:Spherocytosis, Hereditary / Q000378:metabolism / Q000473:pathology; D013154:Spleen / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D007501:Iron; D052203:Ferrosoferric Oxide
keywords: Diffraction; Iron; Magnetic properties; Spleen