title: Expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths.
abstract: BACKGROUND
  It has been studied that the distribution of bone morphogenetic protein 2 is regular under bone defect situation.
  
  
  OBJECTIVE
  To observe the expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths.
  
  
  METHODS
  Forty-eight New Zealand rabbits were divided into two groups randomly. 0.5 cm bone defect and 3.0 cm bone defect were made by wire saw at the middle part of radius bone after anaesthesia.
  
  
  CONCLUSIONS
  Western blot results showed that in the 0.5 cm bone defect group, the expression of bone morphogenetic protein 2 of the tissues in the bone defect site was increased gradually at 1, 3, 4 weeks after operation, and the expression in each defect group was increased when compared with that immediately after injury (P<0.05). In the 3.0 cm bone defect group, the expression of bone morphogenetic protein 2 of tissues in bone defect site was increased gradually and reached to its peak at 3 weeks after the operation (P<0.05). The peak value in the 3.0 cm bone defect group was significantly higher than that in 0.5 cm bone defect group (P<0.05). The peak value was maintained in high level. The comparison of bone callus formation showed that the bone callus formation of 3.0 cm bone defect group was less than that of the 0.5 cm bone defect group at 3 and 4 weeks after operation (P<0.05). The results indicate that expression of the bone morphogenetic protein 2 in 3.0 cm bone defect site is increased significantly, but the expression level cannot make the bone defect heal itself.
authors: Department of Emergency, General Hospital of Pingmei Shenma Medical Group Pingdingshan, Henan, China.
authors: Guo-Zhu
authors: GZ
authors: Hou
authors: Department of Orthopaedics, The Fifth Hospital of Wuhan, The Second Hospital Affiliated of Jianghan University Wuhan, Hubei, China.
authors: Feng
authors: F
authors: Xu
authors: Department of Pain Treatment, The First Affiliated Hospital of Xinjiang Medical University Urumuqi, Xinjiang, China.
authors: Wen-Ju
authors: WJ
authors: Li
authors: Department of Traumatology, Xinjiang Production and Construction Corps Urumuqi, Xinjiang, China.
authors: Xiao-Meng
authors: XM
authors: Zhu
authors: Department of Orthopaedics, The First Affiliated Hospital of Xinjiang Medical University Urumuqi, Xinjiang, China.
authors: Xing-Hua
authors: XH
authors: Song
authors: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors: Yu-Lin
authors: YL
authors: Zhan
keywords: Tissue construction; bone defect; bone healing; bone length; bone tissue construction; rabbit