title: Middle age onset short-term intermittent fasting dietary restriction prevents brain function impairments in male Wistar rats.
abstract: Intermittent fasting dietary restriction (IF-DR) is recently reported to be an effective intervention to retard age associated disease load and to promote healthy aging. Since sustaining long term caloric restriction regimen is not practically feasible in humans, so use of alternate approach such as late onset short term IF-DR regimen which is reported to trigger similar biological pathways is gaining scientific interest. The current study was designed to investigate the effect of IF-DR regimen implemented for 12 weeks in middle age rats on their motor coordination skills and protein and DNA damage in different brain regions. Further, the effect of IF-DR regimen was also studied on expression of energy regulators, cell survival pathways and synaptic plasticity marker proteins. Our data demonstrate that there was an improvement in motor coordination and learning response with decline in protein oxidative damage and recovery in expression of energy regulating neuropeptides. We further observed significant downregulation in nuclear factor kappa B (NF-κB) and cytochrome c (Cyt c) levels and moderate upregulation of mortalin and synaptophysin expression. The present data may provide an insight on how a modest level of short term IF-DR, imposed in middle age, can slow down or prevent the age-associated impairment of brain functions and promote healthy aging by involving multiple regulatory pathways aimed at maintaining energy homeostasis.
authors: Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, MA, USA. rumani@bu.edu.
authors: Rumani
authors: R
authors: Singh
authors: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. shaffi1608@gmail.com.
authors: Shaffi
authors: S
authors: Manchanda
authors: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. taran3588@yahoo.co.in.
authors: Taranjeet
authors: T
authors: Kaur
authors: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. sushilneuro@gmail.com.
authors: Sushil
authors: S
authors: Kumar
authors: Department of Biotechnology, Kanya Maha Vidyalaya, Jalandhar, Punjab, 144001, India. dinesh.biotekk@gmail.com.
authors: Dinesh
authors: D
authors: Lakhanpal
authors: Department of Pharmaceutical, Social and Administrative Sciences, D'Youville College School of Pharmacy, Buffalo, NY, 14201, USA. sukhwinderlakhman@yahoo.com.
authors: Sukhwinder S
authors: SS
authors: Lakhman
authors: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. kgurcharan.neuro@yahoo.com.
authors: Gurcharan
authors: G
authors: Kaur
mesh_terms: D000375:Aging / Q000473:pathology / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology*; D031204:Caloric Restriction / Q000379:methods*; D004249:DNA Damage; D005215:Fasting / Q000502:physiology*; D059648:Kisspeptins / Q000378:metabolism; D008297:Male; D008959:Models, Neurological; D009048:Motor Skills / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009473:Neuronal Plasticity; D009478:Neuropeptide Y / Q000378:metabolism; D018384:Oxidative Stress; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: C491406:Kiss1 protein, rat; D059648:Kisspeptins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y
keywords: Biogerontology; Cognition; Dietary restriction; Energy homeostasis; Motor co-ordination; Synaptic plasticity