title: Antibacterial Activity and Biosensing of PVA-Lysozyme Microbubbles Formed by Pressurized Gyration.
abstract: In this work, the biosensing and antibacterial capabilities of PVA-lysozyme microbubbles have been explored. Gas-filled PVA-lysozyme microbubbles with and without gold nanoparticles in the diameter range of 10 to 250 μm were produced using a single-step pressurized gyration process. Fluorescence microscopy showed the integration of gold nanoparticles on the shell of the microbubbles. Microbubbles prepared with gold nanoparticles showed greater optical extinction values than those without gold nanoparticles, and these values increased with the concentration of the gold nanoparticles. Both types of microbubbles showed antibacterial activity against Gram-negative Escherichia coli (E. coli), with the bubbles containing the gold nanoparticles performing better than the former. The conjugation of the microbubbles with alkaline phosphatase allowed the detection of pesticide paraoxon in aqueous solution, and this demonstrates the biosensing capabilities of these microbubbles.
authors: Department of Mechanical Engineering, University College London , Torrington Place, London WC1E 7JE, U.K.
authors: Suntharavathanan
authors: S
authors: Mahalingam
authors: Department of Mechanical Engineering, University College London , Torrington Place, London WC1E 7JE, U.K.
authors: Zewen
authors: Z
authors: Xu
authors: Department of Mechanical Engineering, University College London , Torrington Place, London WC1E 7JE, U.K.
authors: Mohan
authors: M
authors: Edirisinghe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015374:Biosensing Techniques; D009113:Muramidase / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011312:Pressure
chemical_list: D000900:Anti-Bacterial Agents; D011142:Polyvinyl Alcohol; D009113:Muramidase