title: Niastella gongjuensis sp. nov., isolated from greenhouse soil.
abstract: An aerobic, Gram-stain-negative, non-flagellated, long rod or rod-shaped bacterial strain, 5GH22-11(T), was isolated from a soil sample of a greenhouse in Gongju, Republic of Korea. According to 16S rRNA gene sequence analysis, strain 5GH22-11(T) showed the highest sequence similarities with Niastella populi THYL-44(T) (97.1%), Niastella koreensis GR20-10T (95.7%) and Niastella yeongjuensis GR20-13(T) (95.6%), and < 3% sequence similarity with all other species with validly published names. The phylogenetic tree also showed strain 5GH22-11(T) formed a compact cluster with members of the genus Niastella. DNA-DNA hybridization revealed strain 5GH22-11(T) is a novel species, showing far less than 70% DNA-DNA relatedness with Niastella populi THYL-44(T). Strain 5GH22-11(T) contained iso-C15 : 0 (34.3%), iso-C17 : 0 3-OH (24.8%) and iso-C15 : 1 G (18.2%) as the major fatty acids; phosphatidylethanolamine, six unknown aminolipids, two unknown lipids and one unknown phospholipid as the polar lipids; and MK-7 as the predominant isoprenoid quinone. The DNA G+C content was 41.4 mol%. Based on these phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 5GH22-11(T) represents a novel species of the genus Niastella, for which the name Niastella gongjuensis sp. nov. is proposed. The type strain is 5GH22-11(T) ( = KACC 17339(T) [corrected] = JCM 19941(T)).
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Soo-Jin
authors: SJ
authors: Kim
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Jae-Hyung
authors: JH
authors: Ahn
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Hang-Yeon
authors: HY
authors: Weon
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Seung-Beom
authors: SB
authors: Hong
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Soon-Ja
authors: SJ
authors: Seok
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Jeong-Seon
authors: JS
authors: Kim
authors: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
authors: Soon-Wo
authors: SW
authors: Kwon
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil