title: Site-directed, on-surface assembly of DNA nanostructures.
abstract: Two-dimensional DNA lattices have been assembled from DNA double-crossover (DX) motifs on DNA-encoded surfaces in a site-specific manner. The lattices contained two types of single-stranded protruding arms pointing into opposite directions of the plane. One type of these protruding arms served to anchor the DNA lattice on the solid support through specific hybridization with surface-bound, complementary capture oligomers. The other type of arms allowed for further attachment of DNA-tethered probe molecules on the opposite side of the lattices exposed to the solution. Site-specific lattice assembly and attachment of fluorophore-labeled oligonucleotides and DNA-protein conjugates was demonstrated using DNA microarrays on flat, transparent mica substrates. Owing to their programmable orientation and addressability over a broad dynamic range from the nanometer to the millimeter length scale, such supramolecular architecture might be used for presenting biomolecules on surfaces, for instance, in biosensor applications.
authors: Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Herrmann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany).
authors: Rebecca
authors: R
authors: Meyer
authors: Center for Nanointegration Duisburg-Essen (CENIDE), Center for Medical Biotechnology (ZMB), University of Duisburg-Essen, Universitätstrasse 2, 45117 Essen (Germany).
authors: Barbara
authors: B
authors: Saccà
authors: Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Herrmann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany). niemeyer@kit.edu.
authors: Christof M
authors: CM
authors: Niemeyer
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA; C011934:mica
keywords: DNA structures; microstructures; nanostructures; self-assembly; surfaces