title: Influence of Time of Assay on Behavioral Responses of Laboratory and Field Populations Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) to DEET.
abstract: Knowledge on test conditions that may influence behavioral responses of mosquitoes is critical when excito-repellency tests are conducted. The objective of this study was to investigate the effect of test time differences on normal circadian activity and behavioral responses of field and colonized Aedes aegypti (L.) (=Stegomyia aegypti) and Culex quinquefasciatus Say to DEET, one of the most common synthetic repellent active ingredients available. Two field populations of Ae. aegypti and Cx. quinquefasciatus from Kanchanaburi and Nonthaburi provinces, respectively, and two long-standing laboratory populations, Ae. aegypti obtained from the U.S. Department of Agriculture, and Cx. quinquefasciatus from the Ministry of Public Health, Thailand, were used. Each population was exposed to DEET during two different periods of time (0900-1500 hours) and (2100-0300 hours). Both field and laboratory Cx. quinquefasciatus showed marked differences in spatial repellent escape responses between day and nighttime periods but none in direct contact tests. No significant differences between day and nighttime testing periods were observed with field or laboratory Ae. aegypti, except a higher daytime escape response from noncontact DEET treatment. This study indicates that test time may influence the behavioral avoidance responses and is a potential confounder of excito-repellency evaluations.
authors: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors: Krajana
authors: K
authors: Tainchum
authors: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors: Wanapa
authors: W
authors: Ritthison
authors: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors: Sunaiyana
authors: S
authors: Sathantriphop
authors: Department of Entomology, Faculty of Agriculture, Kasetsart University, Khamphaengsean, 19 Campus, Nakhon Pathom 73140, Thailand.
authors: Somchai
authors: S
authors: Tanasilchayakul
authors: Institut de Recherche pour le Développement (IRD), UMR-MD3, Montpellier 34093, France.
authors: Sylvie
authors: S
authors: Manguin
authors: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand. Department of Public Health and Malaria Control, International SOS, Jl. Kertajasa, Kuala Kencana, Papua 99920, Indonesia.
authors: Michael J
authors: MJ
authors: Bangs
authors: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand. faasthc@ku.ac.th.
authors: Theeraphap
authors: T
authors: Chareonviriyaphap
mesh_terms: D000330:Aedes; D000818:Animals; D001522:Behavior, Animal; D002940:Circadian Rhythm; D003465:Culex; D003671:DEET; D005260:Female; D007302:Insect Repellents
chemical_list: D007302:Insect Repellents; D003671:DEET
keywords: Aedes aegypti; Culex quinquefasciatus; DEET; excito-repellency