title: Kidney-Specific Reduction of Oxidative Phosphorylation Genes Derived from Spontaneously Hypertensive Rat.
abstract: Mitochondrial (Mt) dysfunction contributes to the pathophysiology of renal function and promotes cardiovascular disease such as hypertension. We hypothesize that renal Mt-genes derived from female spontaneously hypertensive rats (SHR) that exhibit hypertension have reduced expression specific to kidney cortex. After breeding a female Okamoto-Aoki SHR (SAP = 188mmHg) with Brown Norway (BN) males (SAP = 100 and 104 mmHg), hypertensive female progeny were backcrossed with founder BN for 5 consecutive generations in order to maintain the SHR mitochondrial genome in offspring that contain over increasing BN nuclear genome. Mt-protein coding genes (13 total) and nuclear transcription factors mediating Mt-gene transcription were evaluated in kidney, heart and liver of normotensive (NT: n = 20) vs. hypertensive (HT: n = 20) BN/SHR-mtSHR using quantitative real-time PCR. Kidney cortex, but not liver or heart Mt-gene expression was decreased ~2-5 fold in 12 of 13 protein encoding genes of HT BN/SHR-mtSHR. Kidney cortex but not liver mRNA expression of the nuclear transcription factors Tfam, NRF1, NRF2 and Pgc1α were also decreased in HT BN/SHR-mtSHR. Kidney cortical tissue of HT BN/SHR-mtSHR exhibited lower cytochrome oxidase histochemical staining, indicating a reduction in renal oxidative phosphorylation but not in liver or heart. These results support the hypothesis that renal cortex of rats with SHR mitochondrial genome has specifically altered renal expression of genes encoding mitochondrial proteins. This kidney-specific coordinated reduction of mitochondrial and nuclear oxidative metabolism genes may be associated with heritable hypertension in SHR.
authors: Department of Biology, University of Kentucky, Lexington, KY, the United States of America.
authors: Jason A
authors: JA
authors: Collett
authors: Department of Biology, University of Kentucky, Lexington, KY, the United States of America.
authors: Jiffin K
authors: JK
authors: Paulose
authors: Department of Biology, University of Kentucky, Lexington, KY, the United States of America.
authors: Vincent M
authors: VM
authors: Cassone
authors: Department of Biology, University of Kentucky, Lexington, KY, the United States of America.
authors: Jeffrey L
authors: JL
authors: Osborn
mesh_terms: D000818:Animals; D001794:Blood Pressure; D015153:Blotting, Western; D005260:Female; D050259:Genes, Mitochondrial; D006973:Hypertension / Q000503:physiopathology*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D009928:Organ Specificity; D010085:Oxidative Phosphorylation; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011914:Rats, Inbred BN; D011918:Rats, Inbred SHR; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009687:Nuclear Proteins; D012333:RNA, Messenger