title: Regulatory RNA-assisted genome engineering in microorganisms.
abstract: Regulatory RNAs are increasingly recognized and utilized as key modulators of gene expression in diverse organisms. Thanks to their modular and programmable nature, trans-acting regulatory RNAs are especially attractive in genome-scale applications. Here we discuss the recent examples in microbial genome engineering implementing various trans-acting RNA platforms, including sRNA, RNAi, asRNA and CRISRP-Cas. In particular, we focus on how the scalable and multiplex nature of trans-acting RNAs has been used to tackle the challenges in creating genome-wide and combinatorial diversity for functional genomics and metabolic engineering applications. Advances in computational design and context-dependent regulation are also discussed for their contribution in improving fine-tuning capabilities of trans-acting RNAs.
authors: Department of Chemical and Biomolecular Engineering, Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, United States.
authors: Tong
authors: T
authors: Si
authors: Department of Chemical and Biomolecular Engineering, Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, United States.
authors: Mohammad
authors: M
authors: HamediRad
authors: Department of Chemical and Biomolecular Engineering, Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, United States; Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States; Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States; Department of Bioengineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States. Electronic address: zhao5@illinois.edu.
authors: Huimin
authors: H
authors: Zhao
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D016680:Genome, Bacterial; D006801:Humans; D012313:RNA / Q000235:genetics*; D034622:RNA Interference
chemical_list: D012313:RNA