title: Development of microsatellite markers in Ilex kaushue (Aquifoliaceae), a medicinal plant species.
abstract: OBJECTIVE
  Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity.
  
  
  RESULTS
  Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. The number of alleles per locus ranged from one to nine across 22 individuals from both cultivated and wild populations. The observed and expected heterozygosity in these two populations ranged from 0.000 to 1.000 and from 0.000 to 0.785, respectively.
  
  
  CONCLUSIONS
  These markers will be useful in studies on genetic diversity of I. kaushue.
authors: Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Naning 530022, People's Republic of China.
authors: Lan-Fang
authors: LF
authors: Qin
authors: Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Naning 530022, People's Republic of China.
authors: Xin-Cheng
authors: XC
authors: Qu
authors: School of Environment and Life Sciences, Guangxi Teacher's Education University, Naning 530001, People's Republic of China.
authors: Gang
authors: G
authors: Hu
authors: Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Naning 530022, People's Republic of China.
authors: Yun-Feng
authors: YF
authors: Huang
authors: Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Naning 530022, People's Republic of China.
authors: Qi-Wei
authors: QW
authors: Zhang
keywords: Aquifoliaceae; Ilex kaushue; genetic diversity; microsatellite marker