title: NEM1 acts as a suppressor of apoptotic phenotypes in LSM4 yeast mutants.
abstract: Saccharomyces cerevisiae mutants in the essential gene LSM4, involved in messenger RNA decapping, and expressing a truncated form of the LSM4 gene of the yeast Kluyveromyces lactis (Kllsm4Δ1), show premature aging accompanied by the presence of typical markers of apoptosis and high sensitivity to oxidative stressing agents. We isolated multicopy extragenic suppressors of these defects, transforming the Kllsm4Δ1 mutant with a yeast DNA library and selecting clones showing resistance to acetic acid. Here we present one of these clones, carrying a DNA fragment containing the NEM1 gene (Nuclear Envelope Morphology protein 1), which encodes the catalytic subunit of the Nem1p-Spo7p phosphatase holoenzyme. Nem1p regulates nuclear growth by controlling phospholipid biosynthesis and it is required for normal nuclear envelope morphology and sporulation. The data presented here correlate the mRNA metabolism with the biosynthesis of phospholipids and with the functionality of the endoplasmic reticulum.
authors: Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy.
authors: Vanessa
authors: V
authors: Palermo
authors: Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy.
authors: Mariarita
authors: M
authors: Stirpe
authors: Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy.
authors: Mirko
authors: M
authors: Torella
authors: Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy.
authors: Claudio
authors: C
authors: Falcone
authors: Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy cristina.mazzoni@uniroma1.it.
authors: Cristina
authors: C
authors: Mazzoni
mesh_terms: D017209:Apoptosis; D017353:Gene Deletion; D015870:Gene Expression; D005816:Genetic Complementation Test; D007716:Kluyveromyces / Q000235:genetics / Q000502:physiology*; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C502372:Nem1 protein, S cerevisiae; D009687:Nuclear Proteins; D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins
keywords: apoptosis; endoplasmic reticulum; mRNA; phospholipids; yeast