title: Insights into teichoic acid biosynthesis by Bifidobacterium bifidum PRL2010.
abstract: Bifidobacteria are colonizers of the human gut, where they are interacting with their host as well as with other members of the intestinal microbiota. Teichoic acids (TAs) have previously been shown to play an important role in modulating microbe-host interactions in the human gut. However, so far, there is a paucity of information regarding the presence of TAs in the cell envelope of bifidobacteria. In silico analyses targeting the chromosomes of all 48 (sub)species that currently represent the genus Bifidobacterium revealed the presence of genes responsible for TA biosynthesis, suggesting that bifidobacteria contain both wall TAs and lipoteichoic acids. Transcriptome analyses of the infant gut commensal Bifidobacterium bifidum PRL2010 highlighted that the transcription of the presumptive TA biosynthetic loci is modulated in response to environmental conditions reflecting those of the human gut. Furthermore, chemical characterization of TAs produced by PRL2010 indicates the presence of lipoteichoic acids.
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43124 Parma, Italy.
authors: Angelo
authors: A
authors: Colagiorgi
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43124 Parma, Italy.
authors: Francesca
authors: F
authors: Turroni
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43124 Parma, Italy.
authors: Leonardo
authors: L
authors: Mancabelli
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43124 Parma, Italy.
authors: Fausta
authors: F
authors: Serafini
authors: Department of Chemistry, University of Parma, 43124 Parma, Italy.
authors: Andrea
authors: A
authors: Secchi
authors: School of Microbiology and the APC Microbiome Institute, National University of Ireland, Cork, Ireland.
authors: Douwe
authors: D
authors: van Sinderen
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43124 Parma, Italy marco.ventura@unipr.it.
authors: Marco
authors: M
authors: Ventura
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics*; D053898:Biosynthetic Pathways / Q000235:genetics*; D003198:Computer Simulation; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000502:physiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D006801:Humans; D007223:Infant; D008070:Lipopolysaccharides / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D019936:Probiotics; D013682:Teichoic Acids / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid
keywords: bifidobacteria; functional genomics; genomics; host–microbe interactions; microbiota