title: Permeation profiles of resveratrol cream delivered through porcine vaginal mucosa: Evaluation of different HPLC stationary phases.
abstract: Trans-resveratrol affects biological systems in a multitude of ways, but its oral bioavailability is remarkably poor due to in vivo metabolization. This drawback has fomented the development of new strategies for systemic delivery, such as transmucosal delivery via the vaginal route, which is our main focus here. In this sense, our pioneering study purposed to evaluate the trans-resveratrol permeation efficacy through this route. For that, we used a previously validated method and tested it with three different stationary phases: a commercial C18 column and two laboratory-made chromatographic columns containing poly(methyloctadecylsiloxane) (PMODS) thermally immobilized onto zirconized silica (Zr-PMODS) or titanized silica (Ti-PMODS). The permeation experiments showed that resveratrol, in the formulation used, was not successfully delivered to the bloodstream - it was actually retained within the vaginal mucosa, which suggests a local use rather a systemic one.
authors: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil; Suprema-Faculdade de Ciências Médicas e da Saúde de Juiz de Fora, 36033-003 Juiz de Fora, MG, Brazil. Electronic address: h.c.polonini@gmail.com.
authors: Hudson Caetano
authors: HC
authors: Polonini
authors: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors: Pedro Paulo
authors: PP
authors: Soldati
authors: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors: Priscila Aparecida
authors: PA
authors: de Almeida
authors: Institute of Chemistry, University of Campinas, P.O. Box 6154, 13083-970, Campinas, SP, Brazil.
authors: Carla Grazieli Azevedo
authors: CG
authors: da Silva
authors: Institute of Chemistry, University of Campinas, P.O. Box 6154, 13083-970, Campinas, SP, Brazil.
authors: Carol Hollingworth
authors: CH
authors: Collins
authors: Grupo de Química Analítica e Quimiometria (GQAQ), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors: Marcone Augusto Leal
authors: MA
authors: de Oliveira
authors: Ortofarma-Quality Control Laboratory, 36120-000, Matias Barbosa, Brazil.
authors: Anderson de Oliveira
authors: Ade O
authors: Ferreira
authors: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: nadiacritt@gmail.com.
authors: Nádia Rezende Barbosa
authors: NR
authors: Raposo
authors: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: marcosbrand2012@gmail.com.
authors: Marcos Antônio Fernandes
authors: MA
authors: Brandão
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D009092:Mucous Membrane / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*; D013552:Swine; D014621:Vagina / Q000378:metabolism*
chemical_list: D013267:Stilbenes; D000077185:Resveratrol
keywords: Laboratory-made HPLC columns; Resveratrol; Transmucosal absorption