title: Curcumin inhibits proliferation of interleukin-22-treated HaCaT cells.
abstract: The interleukin (IL)-23/IL-17A/IL-22 cytokine axis plays a critical role in the pathogenesis of psoriasis, wherein IL-22 effects on epidermal alternations by inhibiting differentiation and inducing keratinocyte proliferation. In this study, we investigated effects of curcumin on IL-22-induced proliferation in a human keratinocyte cell line (HaCaT) in vitro experiment. The HaCaT cells growth was measured by a Cell Counting Kit-8. The cyclin D1 and cyclin E was detected by real-time RT-PCR and weatern blot. The STAT3 and phosphorylation of STAT3 was tested by weatern blot also. Our results show that curcumin exhibited a significant anti-proliferation effect on HaCaT cells, even in the presence of IL-22. Since STAT3 is crucial for IL-22 signal transduction, we examined the level of phosphorylation of STAT3 in all of the experimental groups. As expected, curcumin inhibited IL-22 induced phosphorylation of STAT3; furthermore, curcumin down regulated cyclin D1 and cyclin E. We can reach a conclusion that curcumin can suppress the proliferation of keratinocytes even with IL-22 treatment. Therefore, we have confidence in future curcumin research about psoriasis treatment.
authors: Department of Pharmacy, General Hospital of Lanzhou Military Command Lanzhou 730050, China.
authors: Yi
authors: Y
authors: Zhao
authors: Department of Pharmacy, General Hospital of Jinan Military Command Jinan 250031, China.
authors: Jun
authors: J
authors: Sun
authors: Department of Neurology, 456 Hospital of PLA Jinan 250031, China.
authors: Wei
authors: W
authors: Dou
authors: Department of Pharmacy, Changhai Hospital, The Second Military Medical University Shanghai 200433, China.
authors: Jin-Hong
authors: JH
authors: Hu
keywords: Curcumin; STAT3; cell growth; cyclin D1; cyclin E; keratinocyte