title: Effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) on the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism, and differences in the action of parent BDE-47 and its hydroxylated metabolites, 5-OH-BDE-47 and 6-OH-BDE47, on steroid secretion by luteal cells.
abstract: In this study we determined the effects of BDE-47 on the expression and activity of phase I (CYP2B1/2) and phase II (SULT1A and COMT) enzymes, and assessed the actions of BDE-47 and its metabolites on luteal steroidogenesis. Luteal cells collected during early (ELP), middle (MLP) and late (LLP) luteal phase were exposed to BDE-47 (0.5, 25, and 50ng/ml) or metabolites (2.5, 5 and 25ng/ml). BDE-47 decreased CYP2B1/2 activity and expression but had no effect on SULT1A or COMT. BDE-47 exerted a stimulatory action on estrogen secretion in MLP and an inhibitory in LLP, but had no effect on progesterone secretion. 5-OH-BDE-47 and 6-OH-BDE-47 decreased progesterone, but had no effect on estrogen secretion.
  
  
  CONCLUSIONS
  The inhibitory effect of BDE-47 on CYP2B1/2 suggests the possibility of BDE-47 accumulation in the corpus luteum; by affecting steroid secretion and steroidogenesis enzymes, BDE-47 and its metabolites can be responsible for shortening luteal phase.
authors: Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: ewa.gregoraszczuk@uj.edu.pl.
authors: E L
authors: EL
authors: Gregoraszczuk
authors: Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors: G
authors: G
authors: Dobrzanska
authors: Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors: A
authors: A
authors: Karpeta
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D015239:Arylsulfotransferase / Q000378:metabolism*; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000494:pharmacology*; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism; D013552:Swine
chemical_list: D004967:Estrogens; D055768:Halogenated Diphenyl Ethers; C511295:2,2',4,4'-tetrabromodiphenyl ether; D011374:Progesterone; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019362:Cytochrome P-450 CYP2B1; C025619:steroid 16-beta-hydroxylase; D002394:Catechol O-Methyltransferase; D015239:Arylsulfotransferase
keywords: 5-OH-BDE-47; 6-OH-BDE-47; BDE-47; Corpus luteum; Detoxification enzymes; Steroidogenesis