title: Anionic metabolite profiling by capillary electrophoresis-mass spectrometry using a noncovalent polymeric coating. Orange juice and wine as case studies.
abstract: In several metabolomic studies, it has already been demonstrated that capillary electrophoresis hyphenated to mass spectrometry (CE-MS) can detect an important group of highly polar and ionized metabolites that are overseen by techniques such as NMR, LC-MS and GC-MS, providing complementary information. In this work, we present a strategy for anionic metabolite profiling by CE-MS using a cationic capillary coating. The polymer, abbreviated as PTH, is composed of a poly-(N,N,N',N'-tetraethyldiethylenetriamine, N-(2-hydroxypropyl) methacrylamide, TEDETAMA-co-HPMA (50:50) copolymer. A CE-MS method based on PTH-coating was optimized for the analysis of a group of 16 standard anionic metabolites. Separation was achieved within 12min, with high separation efficiency (up to 92,000 theoretical plates per meter), and good repeatability, namely, relative standard deviation values for migration times and peak areas were below 0.2 and 2.1%, respectively. The optimized method allowed the detection of 87 metabolites in orange juice and 142 metabolites in red wine, demonstrating the good possibilities of this strategy for metabolomic applications.
authors: Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain; CAPES Foundation, Ministry of Education of Brazil, 70040-020 Brasília, DF, Brazil.
authors: Tanize
authors: T
authors: Acunha
authors: Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain. Electronic address: c.simo@csic.es.
authors: Carolina
authors: C
authors: Simó
authors: Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.
authors: Clara
authors: C
authors: Ibáñez
authors: Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, Brazil.
authors: Alberto
authors: A
authors: Gallardo
authors: Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.
authors: Alejandro
authors: A
authors: Cifuentes
mesh_terms: D000838:Anions / Q000737:chemistry*; D032084:Citrus sinensis / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005504:Food Analysis / Q000379:methods*; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000838:Anions; D011108:Polymers
keywords: Anionic metabolite; Capillary electrophoresis–mass spectrometry; Food analysis; Foodomics; Metabolomics; Orange juice; Wine