title: Effectiveness of Quantitative Real Time PCR in Long-Term Follow-up of Chronic Myeloid Leukemia Patients.
abstract: OBJECTIVE
  To determine the use of the Quantitative Real Time PCR (RQ-PCR) assay follow-up with Chronic Myeloid Leukemia (CML) patients.
  
  
  METHODS
  Cross-sectional observational.
  
  
  METHODS
  Izmir Ataturk Education and Research Hospital, Izmir, Turkey, from 2009 to 2013.
  
  
  METHODS
  Cytogenetic, FISH, RQ-PCR test results from 177 CMLpatients' materials selected between 2009 - 2013 years was set up for comparison analysis. Statistical analysis was performed to compare between FISH, karyotype and RQ-PCR results of the patients. Karyotyping and FISH specificity and sensitivity rates determined by ROC analysis compared with RQ-PCR results. Chi-square test was used to compare test failure rates.
  
  
  RESULTS
  Sensitivity and specificity values were determined for karyotyping 17.6 - 98% (p=0.118, p > 0.05) and for FISH 22.5 - 96% (p=0.064, p > 0.05) respectively. FISH sensitivity was slightly higher than karyotyping but there was calculated a strong correlation between them (p < 0.001). RQ-PCR test failure rate did not correlate with other two tests (p > 0.05); however, karyotyping and FISH test failure rate was statistically significant (p < 0.001).
  
  
  CONCLUSIONS
  Besides, the situation needed for karyotype analysis, RQ-PCR assay can be used alone in the follow-up of CMLdisease.
authors: Department of Genetic, Izmir Ataturk Education and Research Hospital, Izmir, Turkey.
authors: Kaan
authors: K
authors: Savasoglu
authors: Department of Hematology, Izmir Ataturk Education and Research Hospital, Izmir, Turkey.
authors: Kadriye Bahriye
authors: KB
authors: Payzin
authors: Department of Hematology, Izmir Ataturk Education and Research Hospital, Izmir, Turkey.
authors: Fusun
authors: F
authors: Ozdemirkiran
authors: Department of Genetics, Institute of Biomedical and Genetic Engineering, Islamabad.
authors: Belgin
authors: B
authors: Berber
mesh_terms: D003430:Cross-Sectional Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012074:Remission Induction; D012680:Sensitivity and Specificity; D014421:Turkey