title: Cranberry extract-enriched diets increase NAD(P)H:quinone oxidoreductase and catalase activities in obese but not in nonobese mice.
abstract: Consumption of antioxidant-enriched diets is 1 method of addressing obesity, which is associated with chronic oxidative stress and changes in the activity/expression of various enzymes. In this study, we hypothesized that the modulation of antioxidant enzymes and redox status through a cranberry extract (CBE)-enriched diet would differ between obese and nonobese mice. The CBE used in this study was obtained from the American cranberry (Vaccinium macrocarpon, Ericaceae), a popular constituent of dietary supplements that is a particularly rich source of (poly)phenols and has strong antioxidant properties. The present study was designed to test and compare the in vivo effects of 28-day consumption of a CBE-enriched diet (2%) on the antioxidant status of nonobese mice and mice with monosodium glutamate-induced obesity. Plasma, erythrocytes, liver, and small intestine were studied concurrently to obtain more complex information. The specific activities, protein, and messenger RNA expression levels of antioxidant enzymes as well as the levels of malondialdehyde and thiol (SH) groups were analyzed. Cranberry extract treatment increased the SH group content in plasma and the glutathione S-transferase activity in the erythrocytes of the obese and nonobese mice. In addition, in the obese animals, the CBE treatment reduced the malondialdehyde content in erythrocytes and increased
  quinone oxidoreductase (liver) and catalase (erythrocytes and small intestine) activities. The elevation of hepatic
  quinone oxidoreductase activity was accompanied by an increase in the corresponding messenger RNA levels. The effects of CBE on the activity of antioxidant enzymes and redox status were more pronounced in the obese mice compared with the nonobese mice.
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic. Electronic address: Iva.Bousova@faf.cuni.cz.
authors: Iva
authors: I
authors: Boušová
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Hana
authors: H
authors: Bártíková
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Petra
authors: P
authors: Matoušková
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Kateřina
authors: K
authors: Lněničková
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Lukáš
authors: L
authors: Zappe
authors: Faculty of Medicine and Dentistry, Palacký University, 775 15 Olomouc, Czech Republic.
authors: Kateřina
authors: K
authors: Valentová
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Barbora
authors: B
authors: Szotáková
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Jan
authors: J
authors: Martin
authors: Faculty of Pharmacy, Charles University in Prague, 500 05, Hradec Králové, Czech Republic.
authors: Lenka
authors: L
authors: Skálová
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002374:Catalase / Q000097:blood / Q000378:metabolism*; D004032:Diet; D004912:Erythrocytes / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D005982:Glutathione Transferase / Q000097:blood; D007421:Intestine, Small / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000097:blood / Q000139:chemically induced / Q000201:enzymology*; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000097:blood; D029799:Vaccinium macrocarpon
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D008315:Malondialdehyde; D002374:Catalase; D016660:NAD(P)H Dehydrogenase (Quinone); C000597536:Noq1 protein, mouse; D005982:Glutathione Transferase
keywords: Anthocyanin; Antioxidant enzymes; Cranberry; Malondialdehyde; Mice; Monosodium glutamate–induced obesity