title: Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation.
abstract: Oleaginous yeast Trichosporon cutaneum CGMCC 2.1374 was found to utilize inulin directly for microbial lipid fermentation without a hydrolysis step. The potential inulinase-like enzyme(s) in T. cutaneum CGMCC 2.1374 were characterized and compared with other inulinase enzymes produced by varied yeast strains. The consolidated bioprocessing (CBP) for lipid accumulated using inulin was optimized with 4.79 g/L of lipid produced from 50 g/L inulin with the lipid content of 33.6% in dry cells. The molecular weight of the enzyme was measured which was close to invertase in Saccharomyces cerevisiae. The study provided information for inulin hydrolyzing enzyme(s) in oleaginous yeasts, as well as a preliminary CBP process for lipid production from inulin feedstock.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
authors: Juan
authors: J
authors: Wang
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. huizhzh@ecust.edu.cn.
authors: Huizhan
authors: H
authors: Zhang
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. jbao@ecust.edu.cn.
authors: Jie
authors: J
authors: Bao
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry*; D014250:Trichosporon / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D008055:Lipids; D006026:Glycoside Hydrolases; C023086:inulinase
keywords: Consolidated bioprocessing; Dry cell weight; Inulin hydrolyzing enzyme; Lipid production; Trichosporon cutaneum