title: Comparative Secretome Analysis of Aspergillus niger, Trichoderma reesei, and Penicillium oxalicum During Solid-State Fermentation.
abstract: Filamentous fungi such as Aspergillus spp., Trichoderma spp., and Penicillium spp. are frequently used to produce high concentrations of lignocellulosic enzymes. This study examined the discrepancies in the compositions and dynamic changes in the extracellular enzyme systems secreted by Aspergillus niger ATCC1015, Trichoderma reesei QM9414, and Penicillium oxalicum 114-2 cultured on corn stover and wheat bran. The results revealed different types and an abundance of monosaccharides and oligosaccharides were released during incubation, which induced the secretion of diverse glycoside hydrolases. Both the enzyme activities and isozyme numbers of the three fungal strains increased with time. A total of 279, 161, and 183 secretory proteins were detected in A. niger, T. reesei, and P. oxalicum secretomes, respectively. In the A. niger secretomes, more enzymes involved in the degradation of (galacto)mannan, xyloglucan, and the backbone of pectin distributed mostly in dicots were detected. In comparison, although P. oxalicum 114-2 hardly secreted any xyloglucanases, the diversities of enzymes involved in the degradation of xylan and β-(1,3;1,4)-D-glucan commonly found in monocots were higher. The cellulase system of P. oxalicum 114-2 was more balanced. The degradation preference provided a new perspective regarding the recomposition of lignocellulosic enzymes based on substrate types.
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China.
authors: Weili
authors: W
authors: Gong
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China.
authors: Huaiqiang
authors: H
authors: Zhang
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China.
authors: Shijia
authors: S
authors: Liu
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China.
authors: Lili
authors: L
authors: Zhang
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China.
authors: Peiji
authors: P
authors: Gao
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China.
authors: Guanjun
authors: G
authors: Chen
authors: The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, Shandong, 250100, China. lswang@sdu.edu.cn.
authors: Lushan
authors: L
authors: Wang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology*; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D043364:Endo-1,4-beta Xylanases
keywords: Aspergillus niger; Glycoside hydrolase; Penicillium oxalicum; Secretome; Solid-state fermentation; Trichoderma reesei