title: Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition.
abstract: Schistosomiasis is a major human parasitic disease with a global impact. Schistosoma japonicum, the most difficult to control, can survive within host veins for decades. Mechanisms of immune evasion by the parasite, including antigenic variation and surface masking, have been implicated but not well defined. In this study, we defined the immunoglobulin-binding proteomes of S. japonicum using human IgG, IgM, and IgE as the molecular bait for affinity purification, followed by protein identification by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Several proteins situated at the tegument of S. japonicum were able to nonselectively bind to the Fc domain of host immunoglobulins, indicating a mechanism for the avoidance of host immune attachment and recognition. The profile of the immunoglobulin-binding proteomes provides further clues for immune evasion mechanisms adopted by S. japonicum.
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.
authors: Chuang
authors: C
authors: Wu
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.
authors: Nan
authors: N
authors: Hou
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.
authors: Xianyu
authors: X
authors: Piao
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.
authors: Shuai
authors: S
authors: Liu
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.
authors: Pengfei
authors: P
authors: Cai
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.
authors: Yan
authors: Y
authors: Xiao
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100730, P.R. China.|Key Laboratory of Zoonosis, The Ministry of Education, Jilin University, Changchun 130062, P.R. China.
authors: Qijun
authors: Q
authors: Chen
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D016000:Cluster Analysis; D015922:Complement C1q / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D008018:Life Cycle Stages / Q000235:genetics; D008297:Male; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D010271:Parasites / Q000235:genetics / Q000254:growth & development; D011485:Protein Binding; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism; D011817:Rabbits; D011961:Receptors, Fc / Q000378:metabolism; D015203:Reproducibility of Results; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*
chemical_list: D007136:Immunoglobulins; D015800:Protozoan Proteins; D011961:Receptors, Fc; D015922:Complement C1q