title: Sodium selenite/selenium nanoparticles (SeNPs) protect cardiomyoblasts and zebrafish embryos against ethanol induced oxidative stress.
abstract: Alcoholic cardiomyopathy is the damage caused to the heart muscles due to high level of alcohol consumption resulting in enlargement and inflammation of the heart. Selenium is an important trace element that is beneficial to human health. Selenium protects the cells by preventing the formation of free radicals in the body. In the present study, protein mediated synthesis of SeNPs was investigated. Two different sizes of SeNPs were synthesized using BSA and keratin. The synthesized SeNPs were characterized by scanning electron microscopy (SEM) with elemental composition analysis Energy Dispersive X-ray spectroscopy(EDX) and X-ray diffraction (XRD). This study demonstrates the in vitro and in vivo antioxidative effects of sodium selenite and SeNPs. Further selenium and SeNPs were evaluated for their ability to protect against 1% ethanol induced oxidative stress in H9C2 cell line. The selenium and SeNPs were found to reduce the 1% ethanol-induced oxidative damage through scavenging intracellular reactive oxygen species. The selenium and SeNPs could also prevent pericardial edema induced ethanol treatment and reduced apoptosis and cell death in zebrafish embryos. The results indicate that selenium and SeNPs could potentially be used as an additive in alcoholic beverage industry to control the cardiomyopathy.
authors: Department of Biotechnology, Kalasalingam University, Krishnankoil 626126, Tamil Nadu, India.
authors: Kalimuthu
authors: K
authors: Kalishwaralal
authors: Department of Biotechnology, Kalasalingam University, Krishnankoil 626126, Tamil Nadu, India.
authors: Subhaschandrabose
authors: S
authors: Jeyabharathi
authors: Department of Biotechnology, Kalasalingam University, Krishnankoil 626126, Tamil Nadu, India.
authors: Krishnan
authors: K
authors: Sundar
authors: Department of Biotechnology, Kalasalingam University, Krishnankoil 626126, Tamil Nadu, India. Electronic address: a.muthukumaran@klu.ac.in.
authors: Azhaguchamy
authors: A
authors: Muthukumaran
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D004487:Edema / Q000473:pathology; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005033:Ethylmaleimide / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism; D053768:Metal Nanoparticles / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D018038:Sodium Selenite / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014961:X-Ray Diffraction; D015027:Zebrafish / Q000196:embryology*
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D000431:Ethanol; D007633:Keratins; D012643:Selenium; D018038:Sodium Selenite; D005033:Ethylmaleimide
keywords: Alcoholic beverages; H9C2 cell line; SeNPs; Sodium selenite; Zebra fish embryo