title: Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ.
abstract: Chicken anemia virus (CAV) is a severe threat to the chicken industry and causes heavy economic losses worldwide. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant VP1 (rVP1) and pigeon interferon-γ (rPiIFN-γ). Results indicated that rPiIFN-γ enhanced humoral immunity elicited by rVP1 as early as 10 day after primary immunization and reach the high titer after secondary immunization. When compared to chickens immunized with rVP1, inactivated vaccine, chickens immunized with rVP1+rPiIFN-γ showed faster and higher levels (p<0.05) of antibody titer. The CAV challenge result showed that the rVP1+rPiIFN-γ vaccine prevent the reducing of hematocrit values in comparison with the rVP1 or inactivated groups. The relative fold inductions of mRNA expression of Th1-type (IFN-γ), but not Th2-type (IL-4) cytokines in splenocytes isolated from chickens immunized with rVP1+rPiIFN-γ were significantly higher than those of the rVP1 or inactivated vaccine groups. In conclusion, our study found that rPiIFN-γ can enhance both humoral and cellular immunity elicited by an rVP1 vaccine. The rVP1+rPiIFN-γ vaccine may provide a new strategy vaccine against CAV in chicken.
authors: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 912 Pingtung, Taiwan.
authors: Sin Ying
authors: SY
authors: Shen
authors: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 912 Pingtung, Taiwan.
authors: Wei Chun
authors: WC
authors: Chang
authors: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 912 Pingtung, Taiwan.
authors: Hsiang Heng
authors: HH
authors: Yi
authors: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, 912 Pingtung, Taiwan.
authors: Shinn-Shong
authors: SS
authors: Tsai
authors: Institute of Molecular Biology, National Chung Hsing University, 40227 Taichung, Taiwan.
authors: Hung Jen
authors: HJ
authors: Liu
authors: Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan.
authors: Pei-Chun
authors: PC
authors: Liao
authors: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 912 Pingtung, Taiwan. Electronic address: kpchuang@mail.npust.edu.tw.
authors: Kuo Pin
authors: KP
authors: Chuang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D030161:Avian Proteins / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D017727:Chicken anemia virus / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D010856:Columbidae / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006400:Hematocrit; D056724:Immunity, Humoral / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D030161:Avian Proteins; D036022:Capsid Proteins; D016207:Cytokines; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011994:Recombinant Proteins; C442548:VP1 protein, Chicken anemia virus; D022223:Vaccines, Subunit; D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: CAV; Pigeon IFN-γ; Subunit vaccine