title: Discovery of a novel fluorescent probe for the sensitive detection of β-amyloid deposits.
abstract: Here we reported the development of the first photoinduced electron transfer (PeT) probe (1) to directly locate β-amyloid aggregates (Aβ plaques) in the brain without the need of post-washing procedures. The probe showed a high affinity for Aβ aggregates with a Kd value of 3.5nM. It is weakly emissive by itself with its fluorescence quenched by electron transfer from PeT donor to the excited fluorophore. But selective binding to Aβ plaques would attenuate the PeT process and restore the fluorescence, therefore facilitating the tracking of Aβ plaques. The probe is advantageous in that its fluorescence is environment-less-sensitive and no washing procedure is required to provide high contrast fluorescent signal when applied to stain brain tissues. As a proof of concept, its application has been exemplified by staining Aβ plaques in slices of brain tissue from double transgenic (APP/PS1) mice of Alzheimer's disease.
authors: State Key Laborarory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors: Wenming
authors: W
authors: Ren
authors: CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors: Mingming
authors: M
authors: Xu
authors: Division of Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital and Department of Radiology, Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
authors: Steven H
authors: SH
authors: Liang
authors: College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang road, Hangzhou, China.
authors: Huaijiang
authors: H
authors: Xiang
authors: College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang road, Hangzhou, China.
authors: Li
authors: L
authors: Tang
authors: College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang road, Hangzhou, China.
authors: Minkui
authors: M
authors: Zhang
authors: State Key Laborarory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors: Dejun
authors: D
authors: Ding
authors: College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang road, Hangzhou, China. Electronic address: lixin81@zju.edu.cn.
authors: Xin
authors: X
authors: Li
authors: CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. Electronic address: hzhang@simm.ac.cn.
authors: Haiyan
authors: H
authors: Zhang
authors: College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang road, Hangzhou, China; State Key Laborarory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. Electronic address: yhhu@zju.edu.cn.
authors: Youhong
authors: Y
authors: Hu
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D015374:Biosensing Techniques; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D051379:Mice; D058225:Plaque, Amyloid / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology
chemical_list: D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes
keywords: Alzheimer's disease; Aβ plaques; Fluorescent probe; Imaging; Photoinduced electron transfer