title: A New Segmental Hepatectomy Approach Using Ultrasound-Guided Portal Branch Infusion of a Thermosensitive Gel in Pigs.
abstract: BACKGROUND
  The aim of this study was to evaluate the safety, feasibility, and efficacy of a new segmental hepatectomy (SH) approach using intraoperative ultrasound (IOUS) guided infusion of a reversible thermosensitive gel into the portal vein branch in pigs;
  
  
  METHODS
  Poloxamer 407 aqueous solution (20%, W/V) was mixed with indocyanine green (P407-ICG) in this study to make it green, and it remained liquid at room temperature and turned into a firm gel upon reaching body temperature. In experiment I, six pigs were used to detect the outcome of infusing the mixture into the biliary tract, liver parenchyma, and hepatic vein for a safety study. In experiment II, another 12 pigs were randomly segmented into two groups [SH group and partial hepatectomy (PH) group] to investigate the feasibility and efficacy of the new approach using IOUS-guided infusion of the mixture into the portal branch;
  
  
  RESULTS
  No thermosensitive gel-induced abnormal changes were observed in the safety study. In the SH group, IOUS-guided infusion of the P407-ICG solution was effective in occluding the portal blood temporarily and demarcating the target liver segment to achieve precise SH. The blood loss in the SH group was significantly less than that of the PH group;
  
  
  CONCLUSIONS
  SH assisted by IOUS-guided infusion of the reversible thermosensitive gel into the feeding portal vein branches is feasible, safe, simple, and effective.
authors: a Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Chinese PLA Medical College , Beijing , China.
authors: Peng-Fei
authors: PF
authors: Wang
authors: a Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Chinese PLA Medical College , Beijing , China.
authors: Chong-Hui
authors: CH
authors: Li
authors: a Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Chinese PLA Medical College , Beijing , China.
authors: Ai-Qun
authors: AQ
authors: Zhang
authors: a Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Chinese PLA Medical College , Beijing , China.
authors: Shou-Wang
authors: SW
authors: Cai
authors: a Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Chinese PLA Medical College , Beijing , China.
authors: Jia-Hong
authors: JH
authors: Dong
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D006498:Hepatectomy / Q000379:methods*; D008297:Male; D020442:Poloxamer / Q000008:administration & dosage*; D011897:Random Allocation; D013552:Swine; D018084:Ultrasonography, Interventional
chemical_list: C539704:LeGoo; D020442:Poloxamer
keywords: hepatic blood inflow occlusion; partial hepatectomy; poloxamer 407; segmental hepatectomy; swine; thermosensitive