title: Intrinsic dorsoventral patterning and extrinsic EGFR signaling genes control glial cell development in the Drosophila nervous system.
abstract: Dorsoventral patterning and epidermal growth factor receptor (EGFR) signaling genes are essential for determining neural identity and differentiation of the Drosophila nervous system. Their role in glial cell development in the Drosophila nervous system is not clearly established. Our study demonstrated that the dorsoventral patterning genes, vnd, ind, and msh, are intrinsically essential for the proper expression of a master glial cell regulator, gcm, and a differentiation gene, repo, in the lateral glia. In addition, we showed that esg is particularly required for their expression in the peripheral glia. These results indicate that the dorsoventral patterning and EGFR signaling genes are essential for identity determination and differentiation of the lateral glia by regulating proper expression of gcm and repo in the lateral glia from the early glial development. In contrast, overexpression of vnd, msh, spi, and Egfr genes repressed the expression of Repo in the ventral neuroectoderm, indicating that maintenance of correct columnar identity along the dorsoventral axis by proper expression of these genes is essential for restrictive formation of glial precursor cells in the lateral neuroectoderm. Therefore, the dorsoventral patterning and EGFR signaling genes play essential roles in correct identity determination and differentiation of lateral glia in the Drosophila nervous system.
authors: Department of Chemistry, Konkuk University, Seoul 143-701, Republic of Korea.
authors: H J
authors: HJ
authors: Kim
authors: Department of Chemistry, Konkuk University, Seoul 143-701, Republic of Korea.
authors: H J
authors: HJ
authors: Ahn
authors: Department of Chemistry, Konkuk University, Seoul 143-701, Republic of Korea.
authors: S
authors: S
authors: Lee
authors: Department of Chemistry, Konkuk University, Seoul 143-701, Republic of Korea.
authors: J H
authors: JH
authors: Kim
authors: Department of Chemistry, Konkuk University, Seoul 143-701, Republic of Korea.
authors: J
authors: J
authors: Park
authors: Department of Biology Education, Seoul National University, Seoul 151-742, Republic of Korea.
authors: S-H
authors: SH
authors: Jeon
authors: Department of Chemistry, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: flier@konkuk.ac.kr.
authors: S H
authors: SH
authors: Kim
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D009420:Nervous System; D055495:Neurogenesis / Q000235:genetics; D009457:Neuroglia / Q000502:physiology*; D018010:Receptors, Invertebrate Peptide / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D018398:Homeodomain Proteins; D018010:Receptors, Invertebrate Peptide; D014157:Transcription Factors; C089972:repo protein, Drosophila; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors
keywords: Drosophila; EGFR signaling genes; development; dorsoventral patterning; glia