title: Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches.
abstract: BACKGROUND
  Allergy to short ragweed (Ambrosia artemisiifolia) pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short ragweed pollen allergens are officially recorded, patterns of IgE reactivity observed in ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short ragweed pollen.
  
  
  METHODS
  Following a Proteomics-Informed-by-Transcriptomics approach, a comprehensive transcriptomic data set was built up from RNA-seq analysis of short ragweed pollen. Mass spectrometry-based proteomic analyses and IgE reactivity profiling after high resolution 2D-gel electrophoresis were then combined to identify novel allergens.
  
  
  RESULTS
  Short ragweed pollen transcripts were assembled after deep RNA sequencing and used to inform proteomic analyses, thus leading to the identification of 573 proteins in the short ragweed pollen. Patterns of IgE reactivity of individual sera from 22 allergic patients were assessed using an aqueous short ragweed pollen extract resolved over 2D-gels. Combined with information derived from the annotated pollen proteome, those analyses revealed the presence of multiple unreported IgE reactive proteins, including new Amb a 1 and Amb a 3 isoallergens as well as 7 novel candidate allergens reacting with IgEs from 20-70% of patients. The latter encompass members of the carbonic anhydrase, enolase, galactose oxidase, GDP dissociation inhibitor, pathogenesis related-17, polygalacturonase and UDP-glucose pyrophosphorylase families.
  
  
  CONCLUSIONS
  We extended the list of allergens identified in short ragweed pollen. These findings have implications for both diagnosis and allergen immunotherapy purposes.
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Véronique
authors: V
authors: Bordas-Le Floch
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Maxime
authors: M
authors: Le Mignon
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Julien
authors: J
authors: Bouley
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Rachel
authors: R
authors: Groeme
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Karine
authors: K
authors: Jain
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Véronique
authors: V
authors: Baron-Bodo
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Emmanuel
authors: E
authors: Nony
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Laurent
authors: L
authors: Mascarell
authors: Stallergenes, 6 rue Alexis de Tocqueville, 92183, Antony cedex, France.
authors: Philippe
authors: P
authors: Moingeon
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D031164:Ambrosia / Q000737:chemistry / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry / Q000276:immunology*; D008297:Male; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D040901:Proteomics; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*
chemical_list: D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E