title: Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual-wavelength fingerprinting mode and random forest.
abstract: The classical traditional Chinese formulation LiuweiDihuang, shown to have clinical efficacy for "nourishing kidney-yin" in traditional Chinese medicine, has been used for thousands of years in China. Little attention, however, has been paid to quality control methods for this formulation. Hence, a rapid and sensitive analytical technique is urgently needed for the evaluation of LiuweiDihuang preparations to assess its quality and pharmacological functionality. In this study, an ultra high performance liquid chromatography dual-wavelength method was developed to simultaneously determine 11 constituents in LiuweiDihuang preparations. This robust approach provided a fast and comprehensive quantitative determination of the major bioactive markers within LiuweiDihuang preparations. To distinguish four dosage forms of LiuweiDihuang preparations, a random forest technique was applied on the spectrometric fingerprint data obtained. This combination approach of chromatographic techniques and data analyses might serve as a rapid and efficient tool to ensure the quality of LiuweiDihuang preparations and other Chinese medicinal formulations and can support quality control and scientific research into the pharmacological potential for these formulations.
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.|Division of Stem Cell Regulation and Application with Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Yixing
authors: Y
authors: Qiu
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.|Research Center of Modernization of Traditional Chinese Medicines, Central South University, Changsha, P.R. China.
authors: Jianhua
authors: J
authors: Huang
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Xingming
authors: X
authors: Jiang
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Yang
authors: Y
authors: Chen
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Yang
authors: Y
authors: Liu
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Rong
authors: R
authors: Zeng
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Nuzhat
authors: N
authors: Shehla
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Qiang
authors: Q
authors: Liu
authors: Division of Stem Cell Regulation and Application with Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Duanfang
authors: D
authors: Liao
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.|Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Science, Shanghai, P.R. China.
authors: Dean
authors: D
authors: Guo
authors: Research Center of Modernization of Traditional Chinese Medicines, Central South University, Changsha, P.R. China.
authors: Yizeng
authors: Y
authors: Liang
authors: TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, P.R. China.|Division of Stem Cell Regulation and Application with Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, P.R. China.
authors: Wei
authors: W
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D057230:Limit of Detection; D025341:Principal Component Analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction
keywords: Dual-wavelength fingerprinting; Liquid chromatography; LiuweiDihuang; Quality control; Random forest