title: An aptamer targeting shared tumor-specific peptide antigen of MAGE-A3 in multiple cancers.
abstract: A DNA aptamer was identified against the shared tumor-specific MAGE-A3111-125 peptide antigen. The dissociation constant between the aptamer and the peptide was measured at 57 nM. Binding of the aptamer to seven types of cancer cells, melanoma, breast, colorectal, liver, lung, pancreas and oral cancer, was confirmed with flow cytometry and fluorescence imaging. Cy3-conjugated aptamers signals were specifically localized to the surface of those cancer cells. The results indicate that the DNA aptamer against the shared tumor-specific MAGE-A3 peptide can be used in cancer cell targeting and has the potential for developing into new modalities for the diagnosis of multiple cancers.
authors: Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors: Chin-Yu
authors: CY
authors: Wang
authors: Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors: Bai-Ling
authors: BL
authors: Lin
authors: Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors: Chung-Hsuan
authors: CH
authors: Chen
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis* / Q000378:metabolism*; D002151:Calorimetry; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D018613:Microscopy, Confocal; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D011485:Protein Binding; D052156:SELEX Aptamer Technique; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D052157:Aptamers, Nucleotide; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins
keywords: SELEX; aptamer; shared tumor-specific antigen