title: High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer.
abstract: Liver cancer is one of the most lethal cancer types in humans, but our understanding of the molecular mechanisms underlying this process remains insufficient. Here, we conducted high-content screening of the potential genes involved in liver cancer metastasis, which we selected from the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, based on the SAMcell method and RNA interference technology. We identified two powerful genes in the liver cancer metastasis process, AEG-1 and AKR1C2, both of which proved to be positive regulators in promoting metastasis in liver cancer. Further clinical results verified their roles in liver cancer. In summary, these findings could provide new insight into the liver cancer mechanism and potentially therapeutic novel targets for liver cancer therapies in the future.
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Cong
authors: C
authors: Li
authors: Department of Infectious Disease, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Xia
authors: X
authors: Wu
authors: Department of Orthopaedic Surgery, Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.
authors: Wei
authors: W
authors: Zhang
authors: Department of Orthopaedic Surgery, Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.
authors: Jia
authors: J
authors: Li
authors: Department of Orthopaedic Surgery, Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.
authors: Huawei
authors: H
authors: Liu
authors: Department of Orthopaedic Surgery, Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.
authors: Ming
authors: M
authors: Hao
authors: Department of Orthopaedic Surgery, Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.
authors: Junsong
authors: J
authors: Wang
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Honghai
authors: H
authors: Zhang
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Gengxia
authors: G
authors: Yang
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Meijun
authors: M
authors: Hao
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Shoupeng
authors: S
authors: Sheng
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Yu
authors: Y
authors: Sun
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Jiang
authors: J
authors: Long
authors: ViewSolid Biotech, Beijing, China.
authors: Juan
authors: J
authors: Li
authors: GenoArray Biotech, Suzhou, China.
authors: Fengfeng
authors: F
authors: Zhuang
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Caixia
authors: C
authors: Hu
authors: Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Li
authors: L
authors: Li
authors: Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China zhengjiasheng66@163.com jiashengzheng@outlook.com.
authors: Jiasheng
authors: J
authors: Zheng
mesh_terms: D015815:Cell Adhesion Molecules / Q000235:genetics*; D045744:Cell Line, Tumor; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000235:genetics*; D008099:Liver / Q000473:pathology*; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D008565:Membrane Proteins; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology*; D034622:RNA Interference / Q000502:physiology; D016601:RNA-Binding Proteins; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
chemical_list: D015815:Cell Adhesion Molecules; D006913:Hydroxysteroid Dehydrogenases; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; C483039:MTDH protein, human; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
keywords: high-content screening; liver cancer; metastasis