title: Accessory enzymes influence cellulase hydrolysis of the model substrate and the realistic lignocellulosic biomass.
abstract: The potential of cellulase enzymes in the developing and ongoing "biorefinery" industry has provided a great motivation to develop an efficient cellulase mixture. Recent work has shown how important the role that the so-called accessory enzymes can play in an effective enzymatic hydrolysis. In this study, three newest Novozymes Cellic CTec cellulase preparations (CTec 1/2/3) were compared to hydrolyze steam pretreated lignocellulosic substrates and model substances at an identical FPA loading. These cellulase preparations were found to display significantly different hydrolytic performances irrelevant with the FPA. And this difference was even observed on the filter paper itself when the FPA based assay was revisited. The analysis of specific enzyme activity in cellulase preparations demonstrated that different accessory enzymes were mainly responsible for the discrepancy of enzymatic hydrolysis between diversified substrates and various cellulases. Such the active role of accessory enzymes present in cellulase preparations was finally verified by supplementation with β-glucosidase, xylanase and lytic polysaccharide monooxygenases AA9. This paper provides new insights into the role of accessory enzymes, which can further provide a useful reference for the rational customization of cellulase cocktails in order to realize an efficient conversion of natural lignocellulosic substrates.
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology & Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: fubaosun@jiangnan.edu.cn.
authors: Fubao Fuebiol
authors: FF
authors: Sun
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology & Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors: Jiapeng
authors: J
authors: Hong
authors: Forestry Products Biotechnology/Bioenergy Group, Wood Science Department, University of British Columbia, 2424 Main Mall, Vancouver BC, V6T 1Z4, Canada.
authors: Jinguang
authors: J
authors: Hu
authors: Forestry Products Biotechnology/Bioenergy Group, Wood Science Department, University of British Columbia, 2424 Main Mall, Vancouver BC, V6T 1Z4, Canada.
authors: Jack N
authors: JN
authors: Saddler
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors: Xu
authors: X
authors: Fang
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology & Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors: Zhenyu
authors: Z
authors: Zhang
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology & Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors: Song
authors: S
authors: Shen
mesh_terms: D018533:Biomass; D001709:Biotechnology; D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000302:isolation & purification / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D008954:Models, Biological; D010209:Paper; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C036909:lignocellulose; D008031:Lignin; C427304:Novozym 188; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: Accessory enzyme (xylanase and AA9); Cellulase mixtures/cocktails; Cellulase preparations (Cellic(®) CTec1/2/3); Enzymatic hydrolysis; Filter paper activity (FPA)