title: Association of Apolipoprotein B and Adiponectin Receptor 1 Genes with Carcass, Bone Integrity and Performance Traits in a Paternal Broiler Line.
abstract: Apolipoprotein B (APOB) and Adiponectin Receptor 1 (ADIPOR1) are related to the regulation of feed intake, fat metabolism and protein deposition and are candidate genes for genomic studies in birds. In this study, associations of two single nucleotide polymorphisms (SNPs) g.102A>T (APOB) and g.729C>T (ADIPOR1) with carcass, bone integrity and performance traits in broilers were investigated. Genotyping was performed on a paternal line of 1,454 broilers. The SNP detection was carried out by PCR-RFLP technique using the restriction enzymes HhaI for the SNP g.729C>T and MslI for the SNP g.102A>T. The association analyses of the two SNPs with 85 traits were performed using the restricted maximum likelihood (REML) and Generalized Quasi-Likelihood Score (GQLS) methods. For REML the model included the random additive genetic effect of animal and fixed effects of sex, hatch and SNP genotypes. In the GQLS method, a logistic regression was used to associate the genotypes with phenotypes adjusted for fixed effects of sex and hatch. The SNP g.729C>T in the ADIPOR1 gene was associated with thickness of the femur and breast skin yield. Thus, the ADIPOR1 gene seems implicated in the metabolism and/or fat deposition and bone integrity in broilers.
authors: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias (FCAV),Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil.
authors: Valdecy Aparecida Rocha
authors: VA
authors: da Cruz
authors: Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada.
authors: Flávio Schramm
authors: FS
authors: Schenkel
authors: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias (FCAV),Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil.
authors: Rodrigo Pelicioni
authors: RP
authors: Savegnago
authors: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias (FCAV),Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil.
authors: Natalia Vinhal
authors: NV
authors: Grupioni
authors: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias (FCAV),Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil.
authors: Nedenia Bonvino
authors: NB
authors: Stafuzza
authors: The Semex Alliance, Guelph, Ontario, Canada.
authors: Mehdi
authors: M
authors: Sargolzaei
authors: Embrapa Suínos e Aves, Concórdia, Santa Catarina, Brasil.
authors: Adriana Mércia Guaratini
authors: AM
authors: Ibelli
authors: Embrapa Suínos e Aves, Concórdia, Santa Catarina, Brasil.
authors: Jane de Oliveira
authors: Jde O
authors: Peixoto
authors: Embrapa Suínos e Aves, Concórdia, Santa Catarina, Brasil.
authors: Mônica Corrêa
authors: MC
authors: Ledur
authors: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias (FCAV),Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil.
authors: Danísio Prado
authors: DP
authors: Munari
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D050218:Body Fat Distribution; D001835:Body Weight / Q000235:genetics*; D002645:Chickens / Q000033:anatomy & histology* / Q000235:genetics* / Q000378:metabolism; D005269:Femur / Q000033:anatomy & histology; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci; D054419:Receptors, Adiponectin / Q000235:genetics*
chemical_list: D001055:Apolipoproteins B; D005819:Genetic Markers; D054419:Receptors, Adiponectin