title: Reduced β-lactoglobulin IgE binding upon in vitro digestion as a result of the interaction of the protein with casein glycomacropeptide.
abstract: The aim of this work was to evaluate the effect of the presence of casein glycomacropeptide (CMP) on the in vitro digestibility and potential allergenicity of β-lactoglobulin (β-lg)-CMP mixtures. The digestion products were analyzed by RP-HPLC and RP-HPLC-ESI-MS/MS. The potential allergenicity of the digestion products was studied by human IgE binding by inhibition ELISA with serum samples from children with clinical allergic symptoms to β-lg. No differences were observed by HPLC in the mixtures hydrolysates due to CMP-β-lg interactions. RP-HPLC-ESI-MS/MS results showed different peptides occurring in the mixtures hydrolysates. Additionally, it was observed a significant reduction of β-lg IgE binding in the presence of CMP. The disappearance of epitopes in the digested mixtures could explain the lower IgE binding observed in these systems compared to β-lg. It can be concluded that the presence of CMP in products containing β-lg may modify the digestion products that may reduce the potential allergenicity of β-lg.
authors: Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina. Electronic address: mjm@di.fcen.uba.ar.
authors: María J
authors: MJ
authors: Martinez
authors: Instituto de Investigación en Ciencias de Alimentación (CIAL), CSIC-UAM, C/ Nicolás Cabrera 9, 28049 Madrid, Spain. Electronic address: gmartos5@gmail.com.
authors: Gustavo
authors: G
authors: Martos
authors: Instituto de Investigación en Ciencias de Alimentación (CIAL), CSIC-UAM, C/ Nicolás Cabrera 9, 28049 Madrid, Spain. Electronic address: e.molina@csic.es.
authors: Elena
authors: E
authors: Molina
authors: Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina. Electronic address: apilosof@di.fcen.uba.ar.
authors: Ana M R
authors: AM
authors: Pilosof
mesh_terms: D000485:Allergens / Q000276:immunology / Q000378:metabolism; D002364:Caseins / Q000378:metabolism* / Q000494:pharmacology; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004063:Digestion; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D007782:Lactoglobulins / Q000276:immunology* / Q000378:metabolism*; D016269:Milk Hypersensitivity / Q000276:immunology; D010446:Peptide Fragments / Q000378:metabolism* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000485:Allergens; D002364:Caseins; D000939:Epitopes; D007782:Lactoglobulins; D010446:Peptide Fragments; C065989:caseinomacropeptide; D007073:Immunoglobulin E
keywords: Allergenicity; Digestion; Interactions