title: Single-Scan Multidimensional NMR Analysis of Mixtures at Sub-Millimolar Concentrations by using SABRE Hyperpolarization.
abstract: Signal amplification by reversible exchange (SABRE) is a promising method to increase the sensitivity of nuclear magnetic resonance (NMR) experiments. However, SABRE-enhanced (1)H NMR signals are short lived, and SABRE is often used to record 1D NMR spectra only. When the sample of interest is a complex mixture, this results in severe overlaps for (1)H spectra. In addition, the use of a co-substrate, whose signals may obscure the (1) H spectra, is currently the most efficient way to lower the detection limit of SABRE experiments. Here, we describe an approach to obtain clean, SABRE-hyperpolarized 2D (1)H NMR spectra of mixtures of small molecules at sub-millimolar concentrations in a single scan. The method relies on the use of para-hydrogen together with a deuterated co-substrate for hyperpolarization and ultrafast 2D NMR for acquisition. It is applicable to all substrates that can be polarized with SABRE.
authors: Laboratoire Structure et Dynamique par Résonance Magnétique, IRAMIS/NIMBE - UMR 3685, CEA/CNRS CEA-Saclay, 91191, Gif sur Yvette, France.
authors: Valeria
authors: V
authors: Daniele
authors: Institut Galien-Paris Sud, UMR CNRS 8612, Faculté de Pharmacie, Université Paris-Sud, 5 rue Jean-Baptiste Clément, 92290, Châtenay-Malabry, France.
authors: François-Xavier
authors: FX
authors: Legrand
authors: Laboratoire Structure et Dynamique par Résonance Magnétique, IRAMIS/NIMBE - UMR 3685, CEA/CNRS CEA-Saclay, 91191, Gif sur Yvette, France.
authors: Patrick
authors: http://orcid.org/0000-0003-4008-2912
authors: P
authors: Berthault
authors: Institut de Chimie des Substances Naturelles-, CNRS UPR2301, Avenue de la Terrasse, 91190, Gif-sur-Yvette, France. jeannicolas.dumez@cnrs.fr.
authors: Jean-Nicolas
authors: JN
authors: Dumez
authors: Laboratoire Structure et Dynamique par Résonance Magnétique, IRAMIS/NIMBE - UMR 3685, CEA/CNRS CEA-Saclay, 91191, Gif sur Yvette, France. gaspard.huber@cea.fr.
authors: Gaspard
authors: G
authors: Huber
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D056831:Coordination Complexes; D006859:Hydrogen / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy
chemical_list: D056831:Coordination Complexes; D015082:2,2'-Dipyridyl; D006859:Hydrogen
keywords: NMR spectroscopy; analytical methods; hyperpolarization; para-hydrogen; ultrafast experiments