title: Magnetic NGF-releasing PLLA/iron oxide nanoparticles direct extending neurites and preferentially guide neurites along aligned electrospun microfibers.
abstract: Nerve growth factor releasing composite nanoparticles (NGF-cNPs) were developed to direct the extension of neurite outgrowth from dorsal root ganglia (DRG). Iron oxide magnetic nanoparticles were incorporated into poly-l-lactic acid (PLLA) nanoparticles in order to position the NGF-cNPs in a culture dish. Neurites growing from DRG extended toward the NGF released from the NGF-cNPs. DRG were then cultured on aligned PLLA microfibers in the presence of NGF-cNPs, and these biomaterials combined to align DRG neurite extension along one axis and preferentially toward the NGF-cNPs. This combinatorial biomaterial approach shows promise as a strategy to direct the extension of regenerating neurites.
authors: Department of Chemistry and Biochemistry, University of California-San Diego , La Jolla, California 92093, United States.
authors: Jonathan M
authors: JM
authors: Zuidema
authors: Christina
authors: C
authors: Provenza
authors: Tyler
authors: T
authors: Caliendo
authors: Institute of Biomedical Engineering and Informatics (BMTI), Technische Universität Ilmenau , G-Kirchhoff-Str. 2, D-98693 Ilmenau, Germany.|Department of Nano Biophotonics, Leibniz Institute of Photonic Technology (IPHT) , A.-Einstein-Str. 9, D-07745 Jena, Germany.
authors: Silvio
authors: S
authors: Dutz
authors: Ryan J
authors: RJ
authors: Gilbert
mesh_terms: D000818:Animals; D049108:Cell Enlargement / Q000187:drug effects; D002642:Chick Embryo; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000737:chemistry; D008159:Lumbar Vertebrae; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D020932:Nerve Growth Factor / Q000008:administration & dosage* / Q000493:pharmacokinetics; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D018696:Neuroprotective Agents; D011091:Polyesters; D011108:Polymers; C000499:ferric oxide; D019344:Lactic Acid; C033616:poly(lactide); D020932:Nerve Growth Factor
keywords: Poly-l-lactic acid; electrospun fiber; magnetic nanoparticle; nerve growth factor; neurite extension