title: [Regulatory Effect of Protein Disulfide Isomerase on Platelet GPIbα Ectodomain Shedding].
abstract: OBJECTIVE
  This study was aimed to investigate the regulatory effect of protein disulfide isomerase (PDI) on platelet GPIbα ectodomain shedding.
  
  
  METHODS
  The washed platelets were obtained from healthy volunteers. Platelets were incubated with PDI inhibitor bacitracin before stimulation with PMA (Phorbol-12-myristate-13-acetate), dibucaine and collagen. The N-terminal domain of GPIbα in supernatant was detected by Western blot, the GPIbα expression and the intraplatelet ROS levels were measured by flow cytometry.
  
  
  RESULTS
  neither GC content nor GPIbα expression was changed after the washed platelets from the healthy donors were incubated only with PDI inhibitor. The washed platelets were incubated with PDI inhibitor before stimulation with different stimulin, PMA, dibucaine or collagen, and then GPIbα was cleaved and ROS levels were elevated more than that in the controls.
  
  
  CONCLUSIONS
  PDI participates in the induced GPIbα ectodomein shedding, and the effect of PDI in this process maybe depend on the change of ROS level inside platelets. These results might provide a new point of view for the platelet drug development.
authors: Jiangsu Institute of Haematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Xia
authors: X
authors: Wu
authors: Jiangsu Institute of Haematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Rong
authors: R
authors: Yan
authors: Jiangsu Institute of Haematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Li-Li
authors: LL
authors: Zhao
authors: Jiangsu Institute of Haematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, Jiangsu Province, China . E-mail: kesdai@hotmail.com.
authors: Ke-Sheng
authors: KS
authors: Dai
mesh_terms: D001792:Blood Platelets; D003094:Collagen; D005434:Flow Cytometry; D006801:Humans; D019038:Platelet Glycoprotein GPIb-IX Complex; D019704:Protein Disulfide-Isomerases
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex; D003094:Collagen; D019704:Protein Disulfide-Isomerases