title: Analyses of the modulatory effects of antibacterial silver doped calcium phosphate-based ceramic nano-powder on proliferation, survival, and angiogenic capacity of different mammalian cells in vitro.
abstract: In this study, the antibacterial, cytotoxic, and angiogenic activities of silver doped calcium phosphate-based inorganic powder (ABT or PAG) were systematically investigated. ABT powders containing varying silver content were fabricated using a wet chemical manufacturing method. Antibacterial efficiencies of the ABT powders were investigated using a standard test with indicator bacteria and yeast. The cytotoxic effects of ABT on three different fibroblast cells and human umbilical vein endothelial cells (HUVECs) were assessed using MTT assay. ABT powder exhibits concentration-related cytotoxicity characteristics. Apoptotic activity, attachment capability, and wound healing effects were examined on fibroblasts. The angiogenic activity of ABT was investigated by tube formation assay in HUVECs; 10 μg ml(-1) and 100 μg ml(-1) concentrations of the highest metal ion content of ABT did not disrupt the tube formation of HUVECs. All these tests showed that ABT does not compromise the survival of the cells and might impose regeneration ability to various cell types. These results indicate that silver doped calcium phosphate-based inorganic powder with an optimal silver content has good potential for developing new biomaterials for implant applications.
authors: Department of Biology, Anadolu University, 26470 Eskisehir, Turkey.
authors: R Beklem
authors: RB
authors: Bostancıoğlu
authors: Ceren
authors: C
authors: Peksen
authors: Hatice
authors: H
authors: Genc
authors: Mevlüt
authors: M
authors: Gürbüz
authors: Filiz Bayrakçı
authors: FB
authors: Karel
authors: A Savas
authors: AS
authors: Koparal
authors: Aydin
authors: A
authors: Dogan
authors: Nusret
authors: N
authors: Kose
authors: A Tansu
authors: AT
authors: Koparal
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D002516:Ceramics / Q000737:chemistry; D003412:Cricetulus; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D011208:Powders; D012834:Silver / Q000008:administration & dosage*
chemical_list: D000900:Anti-Bacterial Agents; D018786:Bone Substitutes; D002130:Calcium Phosphates; D053769:Nanocapsules; D011208:Powders; D012834:Silver; C020243:calcium phosphate