title: Interaction between vitamin B6 and source of selenium on the response of the selenium-dependent glutathione peroxidase system to oxidative stress induced by oestrus in pubertal pig.
abstract: This study aimed to assess the interaction between vitamin B6 and selenium (Se) for the flow of Se towards the Se-dependent glutathione peroxidase (GPX) system in response to oxidative stress naturally induced by oestrus in a pubertal pig model. At first oestrus, forty-five gilts were randomly assigned to the experimental diets (n=9/group): basal diet (CONT); CONT+0.3mg/kg of Na-selenite (MSeB60); MSeB60+10mg/kg of HCl-B6 (MSeB610); CONT+0.3mg/kg of Se-enriched yeast (OSeB60); and OSeB60+10mg/kg of HCl-B6 (OSeB610). Blood samples were collected at each oestrus (long-term profiles), and daily from day -4 to +3 (slaughter) of the fourth oestrus (peri-oestrus profiles) after which liver, kidneys, and ovaries were collected. For long-term profiles, CONT had lower blood Se than Se-supplemented gilts (p<0.01) and OSe was higher than MSe (p<0.01). Lower erythrocyte pyridoxal-5-phosphate was found in B60 than B610 (p<0.01). No treatment effect was observed on GPX activity. For peri-oestrus profiles, treatment effects were similar to long-term profiles. Treatment effects on liver Se were similar to those for long-term blood Se profiles and OSe had higher renal Se concentrations than MSe gilts (p<0.01). Gene expressions of GPX1, GPX3, GPX4, and selenocysteine lyase in liver and kidney were greatest in OSeB610 gilts (p<0.05). These results suggest that dietary B6 modulate the metabolic pathway of OSe towards the GPX system during the peri-oestrus period in pubertal pigs.
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada; Department of Animal Science, Universidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil. Electronic address: danyel.buenodalto@agr.gc.ca.
authors: Danyel Bueno
authors: DB
authors: Dalto
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada; Department of Animal Science, Laval University, Quebec, Quebec G1V 0A6, Canada.
authors: Mélanie
authors: M
authors: Roy
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: isabelle.audet@agr.gc.ca.
authors: Isabelle
authors: I
authors: Audet
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: mariefrance.palin@agr.gc.ca.
authors: Marie-France
authors: MF
authors: Palin
authors: Department of Animal Science, Laval University, Quebec, Quebec G1V 0A6, Canada. Electronic address: frederic.guay@fsaa.ulaval.ca.
authors: Frédéric
authors: F
authors: Guay
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: jerome.lapointe@agr.gc.ca.
authors: Jérôme
authors: J
authors: Lapointe
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: jacques.matte@agr.gc.ca.
authors: J Jacques
authors: JJ
authors: Matte
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004032:Diet; D004971:Estrus / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D007986:Luteinizing Hormone / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011627:Puberty / Q000187:drug effects*; D011732:Pyridoxal Phosphate / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000097:blood / Q000494:pharmacology*; D013997:Time Factors; D014599:Uterus / Q000187:drug effects / Q000378:metabolism; D025101:Vitamin B 6 / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D011732:Pyridoxal Phosphate; D025101:Vitamin B 6; D007986:Luteinizing Hormone; D005979:Glutathione Peroxidase; D012643:Selenium
keywords: Gilt; Glutathione peroxidase; Inorganic selenium; Oestrus; Organic selenium; Pyridoxine