title: Generation of cancer-specific CD8(+) CD69(+) cells inhibits colon cancer growth.
abstract: The therapy of colon cancer (Cca) is poor currently. The abnormality of immune function is recognized in cancer-bearing hosts. This study aims to inhibit Cca growth with the in vivo induction of Cca-specific CD8(+) CD69(+) T cell polarization. In this study, a mouse tumor model was created with a mouse colon cancer (Cca) cell line. The Cca-bearing mice were immunized with Cca extracts and alum (using as an adjuvant). The CD8(+) CD69(+) cell polarization status was analyzed by flow cytometry and enzyme-linked immunosorbent assay. The results showed that a Cca antigen-specific CD8(+) CD69(+) cells were induced in the Cca-bearing mice. The Cca growth was efficiently inhibited by the Cca-specific CD8(+) CD69(+) cell response. The Cca-specific CD8(+) CD69(+) cells were detected in the Cca mass and spleen, which had the ability to induce Cca cell apoptosis. In conclusion, the Cca-specific CD8(+) CD69(+) cell polarization can be induced in Cca-bearing mice, which can efficiently inhibit the Cca growth.
authors: Department of Gastroenterological Surgery, The First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd., Fuzhou 350005, Fujian, China. Electronic address: binrlan@outlook.com.
authors: Bin
authors: B
authors: Lan
authors: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, 29 Xinquan Rd., Fuzhou 350001, Fujian, China.
authors: Jian
authors: J
authors: Zhang
authors: Department of Gastroenterological Surgery, The First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd., Fuzhou 350005, Fujian, China.
authors: Dong
authors: D
authors: Lu
authors: Department of Gastroenterological Surgery, The First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd., Fuzhou 350005, Fujian, China.
authors: Wenqin
authors: W
authors: Li
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000534:Alum Compounds / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D016827:CD8 Antigens / Q000235:genetics / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D019496:Cancer Vaccines / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D045424:Complex Mixtures / Q000494:pharmacology*; D004195:Disease Models, Animal; D015870:Gene Expression; D007114:Immunization; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D013154:Spleen / Q000276:immunology / Q000473:pathology; D000099192:CD69 Antigens
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D019496:Cancer Vaccines; D045424:Complex Mixtures; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C041524:aluminum sulfate
keywords: Antitumor immunity; Apoptosis; CD8 T cell; Cancer mouse model; Colon cancer