title: Transgenic labeling of parvalbumin-expressing neurons with tdTomato.
abstract: Parvalbumin (PVALB)-expressing fast-spiking interneurons subserve important roles in many brain regions by modulating circuit function and dysfunction of these neurons is strongly implicated in neuropsychiatric disorders including schizophrenia and autism. To facilitate the study of PVALB neuron function we need to be able to identify PVALB neurons in vivo. We have generated a bacterial artificial chromosome (BAC) transgenic mouse line expressing the red fluorophore tdTomato under the control of endogenous regulatory elements of the Pvalb gene locus (JAX # 027395). We show that the tdTomato transgene is faithfully expressed relative to endogenous PVALB expression throughout the brain. Furthermore, targeted patch clamp recordings confirm that the labeled populations in neocortex, striatum, and hippocampus are fast-spiking interneurons based on intrinsic properties. This new transgenic mouse line provides a useful tool to study PVALB neuron function in the normal brain as well as in mouse models of psychiatric disease.
authors: McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors: T
authors: T
authors: Kaiser
authors: McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Human Cell Types Department, Allen Institute for Brain Science, 551 North 34th Street, Seattle, WA 98103, USA.
authors: J T
authors: JT
authors: Ting
authors: McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; PhD Program in Experimental Biology and Biomedicine (PDBEB), Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
authors: P
authors: P
authors: Monteiro
authors: McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address: fengg@mit.edu.
authors: G
authors: G
authors: Feng
mesh_terms: D000200:Action Potentials; D000818:Animals; D001921:Brain / Q000378:metabolism; D017930:Genes, Reporter; D007395:Interneurons / Q000378:metabolism* / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008822:Mice, Transgenic; D010320:Parvalbumins / Q000378:metabolism*; D019076:Transgenes
chemical_list: D008164:Luminescent Proteins; D010320:Parvalbumins
keywords: BAC; interneuron; parvalbumin; psychiatric disorders; tdTomato; transgenic