title: Analysis of Single Locus Trajectories for Extracting In Vivo Chromatin Tethering Interactions.
abstract: Is it possible to extract tethering forces applied on chromatin from the statistics of a single locus trajectories imaged in vivo? Chromatin fragments interact with many partners such as the nuclear membrane, other chromosomes or nuclear bodies, but the resulting forces cannot be directly measured in vivo. However, they impact chromatin dynamics and should be reflected in particular in the motion of a single locus. We present here a method based on polymer models and statistics of single trajectories to extract the force characteristics and in particular when they are generated by the gradient of a quadratic potential well. Using numerical simulations of a Rouse polymer and live cell imaging of the MAT-locus located on the yeast Saccharomyces cerevisiae chromosome III, we recover the amplitude and the distance between the observed and the interacting monomer. To conclude, the confined trajectories we observed in vivo reflect local interaction on chromatin.
authors: Institute for Medical Engineering & Science, The Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America.
authors: Assaf
authors: A
authors: Amitai
authors: Laboratory of genetic instability and nuclear organization, CEA, Fontenay-aux-Roses, France.
authors: Mathias
authors: M
authors: Toulouze
authors: Laboratory of genetic instability and nuclear organization, CEA, Fontenay-aux-Roses, France.
authors: Karine
authors: K
authors: Dubrana
authors: IBENS, Ecole Normale Supérieure, Paris, France and Mathematical Institute, University of Oxford, Oxford, United Kingdom.
authors: David
authors: D
authors: Holcman
mesh_terms: D002843:Chromatin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D008958:Models, Molecular; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D002843:Chromatin