title: Direct Visualization of Walking Motions of Photocontrolled Nanomachine on the DNA Nanostructure.
abstract: A light-driven artificial molecular nanomachine was constructed based on DNA scaffolding. Pyrene-modified walking strands and disulfide bond-connected stator strands, employed as anchorage sites to support walker movement, were assembled into a 2D DNA tile. Pyrene molecules excited by photoirradiation at 350 nm induced cleavage of disulfide bond-connected stator strands, enabling the DNA walker to migrate from one cleaved stator to the next on the DNA tile. The time-dependent movement of the walker was observed and the entire walking process of the walker was characterized by distribution of the walker-stator duplex at four anchorage sites on the tile under different irradiation times. Importantly, the light-fuelled mechanical movements on DNA tile were first visualized in real time during UV irradiation using high-speed atomic force microscopy (HS-AFM).
authors: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University , Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.|Department of Chemistry, Graduate School of Science, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Yangyang
authors: Y
authors: Yang
authors: Department of Chemistry, Graduate School of Science, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Marisa A
authors: MA
authors: Goetzfried
authors: Department of Chemistry, Graduate School of Science, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Kumi
authors: K
authors: Hidaka
authors: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Molecular Engineering and Theranostics, Hunan University , Changsha, Hunan 410082, China.|Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors: Mingxu
authors: M
authors: You
authors: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Molecular Engineering and Theranostics, Hunan University , Changsha, Hunan 410082, China.|Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors: Weihong
authors: W
authors: Tan
authors: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University , Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.|Department of Chemistry, Graduate School of Science, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Hiroshi
authors: H
authors: Sugiyama
authors: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University , Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Masayuki
authors: M
authors: Endo
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D055668:Photochemical Processes
chemical_list: D004247:DNA
keywords: DNA motor; DNA origami; high-speed AFM; light-driven; single molecule analysis