title: Endophytic Fungus from Sinopodophyllum emodi (Wall.) Ying that Produces Podophyllotoxin.
abstract: The aryltetralin lactone podophyllotoxin, which exhibits pronounced antineoplastic activity, is used as the precursor of the following three clinical anticancer drugs: Etoposide™, Etopophos™ and Teniposide™. The natural occurrence of this arylnaphthalene lignan is scarce and unable to meet the ever-rising demand in the medical industry. Thus, developing alternative sources for the production of podophyllotoxin is extremely urgent. This is the first report of the production of podophyllotoxin from endophytic Alternaria tenuissima isolated from Sinopodophyllum emodi (Wall.) Ying. The identification of podophyllotoxin was performed using high-performance liquid chromatography and liquid chromatography-mass spectrometry (MS)-MS and confirmed by comparison with authentic standards.
authors: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
authors: Zizhen
authors: Z
authors: Liang
authors: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
authors: Jia
authors: J
authors: Zhang
authors: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
authors: Xuan
authors: X
authors: Zhang
authors: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
authors: Jinjie
authors: J
authors: Li
authors: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
authors: Xiaoqian
authors: X
authors: Zhang
authors: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing 100875, China cqzhao163@163.com.
authors: Changqi
authors: C
authors: Zhao
mesh_terms: D000528:Alternaria / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D028141:Berberidaceae / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D060026:Endophytes / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D011034:Podophyllotoxin / Q000032:analysis / Q000378:metabolism*
chemical_list: D011034:Podophyllotoxin