title: Accurate detection of binary toxin producer from Clostridium difficile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Thirsty-six binary toxin producers were detected with 2 genotypes (cdtA(+) and cdtB(+)) among 265 Clostridium difficile isolates by multiplex PCR. The rate of accurate differentiation between these 2 genotypes was 100% by 6-peak cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.
authors: Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
authors: Shu-Fang
authors: SF
authors: Kuo
authors: Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors: Tsu-Lan
authors: TL
authors: Wu
authors: Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
authors: Huey-Ling
authors: HL
authors: You
authors: Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
authors: Chun-Chih
authors: CC
authors: Chien
authors: Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors: Ju-Hsin
authors: JH
authors: Chia
authors: Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; Chang Gung University, College of Medicine, Kaohsiung, Taiwan. Electronic address: lee900@adm.cgmh.org.tw.
authors: Chen-Hsiang
authors: CH
authors: Lee
mesh_terms: D036002:ADP Ribose Transferases / Q000032:analysis* / Q000145:classification* / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000145:classification* / Q000235:genetics; D016360:Clostridioides difficile / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D036002:ADP Ribose Transferases; C410164:actin-specific ADP-ribosyltransferase, Clostridium
keywords: Binary toxin; C. difficile; MALDI-TOF