title: Detection of 19 types of para-arachidonic acids in five types of plasma/serum by ultra performance liquid chromatography-tandem mass spectrometry.
abstract: The aim of this study was to examine the consistency of ultra performance liquid chromatography-tandem mass spectrometry (UPLC-TMS) in detecting the levels of para-arachidonic acids (PAAs) among differently processed plasma/serum samples. Ethylenediaminetetraacetic acid (EDTA)-K2, sodium citrate, heparin lithium, coagulant/separation gel, and coagulant-free vacuum blood-sampling tubes were used to collect the fasting blood samples from 15 volunteers. All blood samples were subjected to solid-phase extraction using an Oasis HLB μElution 96-well plate, and UPLC-TMS was used to detect 19 types of PAAs in the blood samples. Within the plasma samples, the concentrations of 5, 6-DHET; 11, 12-epoxyeicosatrienoic acid (EET); 5-hydroxyeicosatetraenoic acid (HETE); leukotriene B4 (LTB4); plasma thromboxane B2 (TXB2); and 12-HETE were significantly higher in the heparin lithium group than in the EDTA-K2 and sodium citrate groups. Within the serum samples, the concentration of LTB4 was significantly higher in the coagulant/separation gel group than in the coagulant-free group, while that of TXB2 was significantly higher in the coagulant-free group than in the coagulant/separation gel group. The levels of some types of PAAs in differently processed plasma/serum samples were inconsistent, and the concentrations of 5, 6-DHET; 5-HETE; 12-HETE; TXB2; and LTB4 were significantly higher in the two serum samples and the heparin lithium group than in the EDTA-K2 and sodium citrate groups.
authors: Department of Clinical Laboratory, Dali Autonomous Prefecture People's Hospital Dali 671000, China.
authors: Anxiong
authors: A
authors: Long
authors: Department of Clinical Laboratory, Dali Autonomous Prefecture People's Hospital Dali 671000, China.
authors: Guoliang
authors: G
authors: Zhong
authors: Department of Clinical Laboratory, Shanghai Baoshan Branch of Shanghai First People's Hospital Shanghai 200940, China.
authors: Qian
authors: Q
authors: Li
authors: Institute of Pediatrics Research, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, China.
authors: Na
authors: N
authors: Lin
authors: Institute of Pediatrics Research, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, China.
authors: Xia
authors: X
authors: Zhan
authors: Department of Clinical Laboratory, The Affiliated Hospital of School of Medicine of Ningbo University Ningbo 315020, China.
authors: Shuaijun
authors: S
authors: Lu
authors: Department of Clinical Laboratory, Jiujiang First People's Hospital Jiujiang 332000, China.
authors: Yuli
authors: Y
authors: Zhu
authors: Department of Clinical Laboratory, Shanghai Baoshan Branch of Shanghai First People's Hospital Shanghai 200940, China.
authors: Liansheng
authors: L
authors: Jiang
authors: Department of Clinical Laboratory, Shanghai Baoshan Branch of Shanghai First People's Hospital Shanghai 200940, China.
authors: Longyi
authors: L
authors: Tan
keywords: Ultra performance liquid chromatography-tandem mass spectrometry; arachidonic acid; para-arachidonic acid; plasma; serum