title: Insight into the transcriptome of Arthrobotrys conoides using high throughput sequencing.
abstract: Arthrobotrys conoides is a nematode-trapping fungus belonging to Orbiliales, Ascomycota group, and traps prey nematodes by means of adhesive network. Fungus has a potential to be used as a biocontrol agent against plant parasitic nematodes. In the present study, we characterized the transcriptome of A. conoides using high-throughput sequencing technology and characterized its virulence unigenes. Total 7,255 cDNA contigs with an average length of 425 bp were generated and 6184 (61.81%) transcripts were functionally annotated and characterized. Majority of unigenes were found analogous to the genes of plant pathogenic fungi. A total of 1749 transcripts were found to be orthologous with eukaryotic proteins of KOG database. Several carbohydrate active enzymes and peptidases were identified. We also analyzed classically and nonclassically secreted proteins and confirmed by BLASTP against fungal secretome database. A total of 916 contigs were analogous to 556 unique proteins of Pathogen Host Interaction (PHI) database. Further, we identified 91 unigenes homologous to the database of fungal virulence factor (DFVF). A total of 104 putative protein kinases coding transcripts were identified by BLASTP against KinBase database, which are major players in signaling pathways. This study provides a comprehensive look at the transcriptome of A. conoides and the identified unigenes might have a role in catching and killing prey nematodes by A. conoides.
authors: Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New V.V. Nagar, Anand, Gujarat, India.|Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand, Gujarat, India.
authors: Pandit
authors: P
authors: Ramesh
authors: Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New V.V. Nagar, Anand, Gujarat, India.
authors: Patel
authors: P
authors: Reena
authors: Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand, Gujarat, India.
authors: Mohapatra
authors: M
authors: Amitbikram
authors: Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand, Gujarat, India.
authors: Joshi
authors: J
authors: Chaitanya
authors: Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New V.V. Nagar, Anand, Gujarat, India.
authors: Kunjadia
authors: K
authors: Anju
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D016000:Cluster Analysis; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D054884:Host-Pathogen Interactions; D009348:Nematoda / Q000382:microbiology; D017422:Sequence Analysis, DNA; D059467:Transcriptome; D037521:Virulence Factors / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D037521:Virulence Factors
keywords: Arthrobotrys conoides; Nematophagous fungi; Secretome; Transcriptome; Virulence genes