title: Cryptosporidium: a first step toward tractability.
abstract: The child-killer and famously recalcitrant parasite Cryptosporidium is the latest organism to yield to the magic of CRISPR/Cas9. The ability to knockout genes and introduce markers promises a new heyday for drug discovery and vaccine development as well as the basic biology of these fascinating parasites.
authors: Departments of Biology and Chemistry, Brandeis University, MS009, 415 South St, Waltham, MA 02453, USA. Electronic address: hedstrom@brandeis.edu.
authors: Lizbeth
authors: L
authors: Hedstrom
mesh_terms: D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D003458:Cryptosporidium / Q000235:genetics / Q000502:physiology*; D055808:Drug Discovery; D041981:Gastrointestinal Tract / Q000469:parasitology; D055786:Gene Knockout Techniques; D006801:Humans; D008018:Life Cycle Stages / Q000502:physiology
keywords: CRISPR/Cas9; gene knockout; reporter parasites; target validation