title: Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed.
abstract: The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (αs2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: αs2-casein A = 2.9 ± 0.8 g/L and F = 1.8 ± 0.4 g/L; β-casein C = 3.0 ± 0.8 g/L and C1 = 2.0 ± 0.7 g/L and κ-casein A = 1.6 ± 0.3 g/L and B = 1.1 ± 0.2 g/L. A good correlation was found between the quantities of αs2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.
authors: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze - Parco d'Orleans, 90128 Palermo, Italy. Electronic address: maria.montalbano@unipa.it.
authors: Maria
authors: M
authors: Montalbano
authors: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze - Parco d'Orleans, 90128 Palermo, Italy.
authors: Roberta
authors: R
authors: Segreto
authors: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze - Parco d'Orleans, 90128 Palermo, Italy.
authors: Rosalia
authors: R
authors: Di Gerlando
authors: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze - Parco d'Orleans, 90128 Palermo, Italy.
authors: Salvatore
authors: S
authors: Mastrangelo
authors: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze - Parco d'Orleans, 90128 Palermo, Italy.
authors: Maria Teresa
authors: MT
authors: Sardina
mesh_terms: D000483:Alleles; D000818:Animals; D002364:Caseins / Q000737:chemistry* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D014644:Genetic Variation; D005838:Genotype; D006041:Goats / Q000145:classification / Q000235:genetics*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D002364:Caseins
keywords: Caseins; Genetic variants; Goat milk; HPLC