title: Activity-based profiling of the proteasome pathway during hepatitis C virus infection.
abstract: Hepatitis C virus (HCV) infection often leads to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. The stability of the HCV proteins is controlled by ubiquitin-dependent and ubiquitin-independent proteasome pathways. Many viruses modulate proteasome function for their propagation. To examine the interrelationship between HCV and the proteasome pathways we employed a quantitative activity-based protein profiling method. Using this approach we were able to quantify the changes in the activity of several proteasome subunits and found that proteasome activity is drastically reduced by HCV replication. The results imply a link between the direct downregulation of the activity of this pathway and chronic HCV infection.
authors: Life Sciences Division, National Research Council of Canada, Ottawa, Canada.|Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.
authors: Neda
authors: N
authors: Nasheri
authors: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.
authors: Zhibin
authors: Z
authors: Ning
authors: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.|Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Canada.
authors: Daniel
authors: D
authors: Figeys
authors: Department of Chemistry, National University of Singapore, Singapore.
authors: Shao
authors: S
authors: Yao
authors: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.|Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Canada.
authors: Natalie K
authors: NK
authors: Goto
authors: Life Sciences Division, National Research Council of Canada, Ottawa, Canada.|Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.|Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Canada.
authors: John Paul
authors: JP
authors: Pezacki
mesh_terms: D045744:Cell Line, Tumor; D016174:Hepacivirus / Q000187:drug effects / Q000502:physiology*; D006526:Hepatitis C / Q000378:metabolism / Q000821:virology; D006518:Hepatitis Viruses / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology; D000077403:Orlistat; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D015398:Signal Transduction; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D007527:Isoenzymes; D007783:Lactones; D020543:Proteome; D014764:Viral Proteins; D000077403:Orlistat; D046988:Proteasome Endopeptidase Complex
keywords: Activity-based protein profiling; Functional proteomics; Hepatitis C virus; Microbiology; Proteasome; Quantitative dimethyl labeling