title: Deletion of Kinin B2 Receptor Alters Muscle Metabolism and Exercise Performance.
abstract: Metabolic syndrome is a cluster of metabolic risk factors such as obesity, diabetes and cardiovascular diseases. Mitochondria is the main site of ATP production and its dysfunction leads to decreased oxidative phosphorylation, resulting in lipid accumulation and insulin resistance. Our group has demonstrated that kinins can modulate glucose and lipid metabolism as well as skeletal muscle mass. By using B2 receptor knockout mice (B2R-/-) we investigated whether kinin action affects weight gain and physical performance of the animals. Our results show that B2R-/- mice are resistant to high fat diet-induced obesity, have higher glucose tolerance as well as increased mitochondrial mass. These features are accompanied by higher energy expenditure and a lower feed efficiency associated with an increase in the proportion of type I fibers and intermediary fibers characterized by higher mitochondrial content and increased expression of genes related to oxidative metabolism. Additionally, the increased percentage of oxidative skeletal muscle fibers and mitochondrial apparatus in B2R-/- mice is coupled with a higher aerobic exercise performance. Taken together, our data give support to the involvement of kinins in skeletal muscle fiber type distribution and muscle metabolism, which ultimately protects against fat-induced obesity and improves aerobic exercise performance.
authors: Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Felipe C G
authors: FC
authors: Reis
authors: Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Anderson S
authors: AS
authors: Haro
authors: School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil.
authors: Aline V N
authors: AV
authors: Bacurau
authors: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors: Sandro M
authors: SM
authors: Hirabara
authors: Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Frederick
authors: F
authors: Wasinski
authors: CEDEME, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Milene S
authors: MS
authors: Ormanji
authors: K.G. Jebsen Center of Exercise in Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors: José B N
authors: JB
authors: Moreira
authors: Department of Neurology and Neurosurgery, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Beatriz H
authors: BH
authors: Kiyomoto
authors: CEDEME, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Clelia R A
authors: CR
authors: Bertoncini
authors: School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil.
authors: Patricia C
authors: PC
authors: Brum
authors: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors: Rui
authors: R
authors: Curi
authors: Max-Delbrück-Center for Molecular Medicine (MDC), Berlin, Germany.
authors: Michael
authors: M
authors: Bader
authors: School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, Brazil.
authors: Reury F P
authors: RF
authors: Bacurau
authors: Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: João B
authors: JB
authors: Pesquero
authors: Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Ronaldo C
authors: RC
authors: Araújo
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D015870:Gene Expression / Q000502:physiology; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D010101:Oxygen Consumption / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D043782:Receptor, Bradykinin B2 / Q000502:physiology*
chemical_list: D007328:Insulin; D020738:Leptin; D043782:Receptor, Bradykinin B2