title: A polysaccharide from cultured mycelium of Hericium erinaceus and its anti-chronic atrophic gastritis activity.
abstract: A polysaccharide named EP-1 was found by screening cultured mycelium of Hericium erinaceus, which was extracted and subjected to precipitation with ethanol, hollow-fiber ultrafiltration and ion-exchange chromatography. The polysaccharide has a molecular weight of approximately 3100Da and is composed of glucose, mannose and galactose, thus being a heteroglycan. EP-1 has a backbone of α-d-Glc(1→3) and β-d-Glc(1→3). The β-d-Glc(1→3) and α-d-Gal-(1→3) were regarded as branches attached to the C-4 position. The α-d-Man was regarded as a terminal residue. The anti-CAG activity was evaluated in experimental systems using a cell model for identification. The polysaccharide significantly inhibited the growth of MC cells obtained from human gastric mucosa epithelium (GES-1) cells transformed by MNNG, which were used as a chronic atrophic gastritis cell model. It also interfered with the MC cells by inducing cell cycle arrest. Thus, EP-1 shows potential for the development of new functional foods and drugs.
authors: Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130117, China; International Collaborative Research Center, Changchun University of Chinese Medicine, Changchun 130117, China.
authors: Mingxing
authors: M
authors: Wang
authors: Research and Development Center, Changchun University of Chinese Medicine, Changchun 130117, China.
authors: Yang
authors: Y
authors: Gao
authors: Research and Development Center, Changchun University of Chinese Medicine, Changchun 130117, China.
authors: Duoduo
authors: D
authors: Xu
authors: International Collaborative Research Center, Changchun University of Chinese Medicine, Changchun 130117, China; Research and Development Center, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: gaoqipin@sina.com.
authors: Qipin
authors: Q
authors: Gao
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D005757:Gastritis, Atrophic / Q000188:drug therapy / Q000473:pathology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D025282:Mycelium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002241:Carbohydrates; D062610:Fungal Polysaccharides
keywords: Anti-CAG activity; Hericium erinaceus; Polysaccharide