title: Studies on the regulatory effect of Peony-Glycyrrhiza Decoction on prolactin hyperactivity and underlying mechanism in hyperprolactinemia rat model.
abstract: Clinical trials have demonstrated the beneficial effects of Peony-Glycyrrhiza Decoction (PGD) in alleviating antipsychotic-induced hyperprolactinemia (hyperPRL) in schizophrenic patients. In previous experiment, PGD suppressed prolactin (PRL) level in MMQ cells, involving modulating the expression of D2 receptor (DRD2) and dopamine transporter (DAT). In the present study, hyperPRL female rat model induced by dopamine blocker metoclopramide (MCP) was applied to further confirm the anti-hyperpPRL activity of PGD and underlying mechanism. In MCP-induced hyperPRL rats, the elevated serum PRL level was significantly suppressed by either PGD (2.5-10 g/kg) or bromocriptine (BMT) (0.6 mg/kg) administration for 14 days. However, in MCP-induced rats, only PGD restored the under-expressed serum progesterone (P) to control level. Both PGD and BMT administration restore the under-expression of DRD2, DAT and TH resulted from MCP in pituitary gland and hypothalamus. Compared to untreated group, hyperPRL animals had a marked reduction on DRD2 and DAT expression in the arcuate nucleus. PGD (10 g/kg) and BMT (0.6 mg/kg) treatment significant reversed the expression of DRD2 and DAT. Collectively, the anti-hyperPRL activity of PGD associates with the modulation of dopaminergic neuronal system and the restoration of serum progesterone level. Our finding supports PGD as an effective agent against hyperPRL.
authors: School of life sciences, Jilin University, Changchun 130012, China; School of Chinese Medicine, LKS Faculty of Medicine, the University of Hong Kong, Hong Kong, China.
authors: Di
authors: D
authors: Wang
authors: School of Chinese Medicine, LKS Faculty of Medicine, the University of Hong Kong, Hong Kong, China.
authors: Wei
authors: W
authors: Wang
authors: School of life sciences, Jilin University, Changchun 130012, China.
authors: Yulin
authors: Y
authors: Zhou
authors: School of life sciences, Jilin University, Changchun 130012, China.
authors: Juan
authors: J
authors: Wang
authors: School of life sciences, Jilin University, Changchun 130012, China.
authors: Dongxu
authors: D
authors: Jia
authors: School of Chinese Medicine, LKS Faculty of Medicine, the University of Hong Kong, Hong Kong, China.
authors: Hei Kiu
authors: HK
authors: Wong
authors: School of Chinese Medicine, LKS Faculty of Medicine, the University of Hong Kong, Hong Kong, China. Electronic address: zhangzj@hku.hk.
authors: Zhang-Jin
authors: ZJ
authors: Zhang
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000009:adverse effects; D065127:Dopamine D2 Receptor Antagonists / Q000009:adverse effects; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D005260:Female; D006035:Glycyrrhiza / Q000737:chemistry*; D006966:Hyperprolactinemia / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy*; D007031:Hypothalamus / Q000378:metabolism; D008787:Metoclopramide / Q000009:adverse effects; D029596:Paeonia / Q000737:chemistry*; D010902:Pituitary Gland / Q000378:metabolism; D010936:Plant Extracts / Q000627:therapeutic use*; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood*; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014150:Antipsychotic Agents; D065127:Dopamine D2 Receptor Antagonists; D050483:Dopamine Plasma Membrane Transport Proteins; D010936:Plant Extracts; D017448:Receptors, Dopamine D2; D011374:Progesterone; D011388:Prolactin; D014446:Tyrosine 3-Monooxygenase; D008787:Metoclopramide
keywords: Antipsychotics; Dopamine system; Herbal medicine; Hyperprolactinemia; Peony-Glycyrrhiza Decoction (PGD); Sexual hormone