title: Transcriptome sequencing and development of an expression microarray platform for liver infection in adenovirus type 5-infected Syrian golden hamsters.
abstract: The Syrian golden hamster is an attractive animal for research on infectious diseases and other diseases. We report here the sequencing, assembly, and annotation of the Syrian hamster transcriptome. We include transcripts from ten pooled tissues from a naïve hamster and one stimulated with lipopolysaccharide. Our data set identified 42,707 non-redundant transcripts, representing 34,191 unique genes. Based on the transcriptome data, we generated a custom microarray and used this new platform to investigate the transcriptional response in the Syrian hamster liver following intravenous adenovirus type 5 (Ad5) infection. We found that Ad5 infection caused a massive change in regulation of liver transcripts, with robust up-regulation of genes involved in the antiviral response, indicating that the innate immune response functions in the host defense against Ad5 infection of the liver. The data and novel platforms developed in this study will facilitate further development of this important animal model.
authors: Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology, 1100 S. Grand Boulevard, St. Louis, MO 63104, United States.
authors: Baoling
authors: B
authors: Ying
authors: Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology, 1100 S. Grand Boulevard, St. Louis, MO 63104, United States.
authors: Karoly
authors: K
authors: Toth
authors: Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology, 1100 S. Grand Boulevard, St. Louis, MO 63104, United States.
authors: Jacqueline F
authors: JF
authors: Spencer
authors: Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology, 1100 S. Grand Boulevard, St. Louis, MO 63104, United States.
authors: Rajeev
authors: R
authors: Aurora
authors: Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology, 1100 S. Grand Boulevard, St. Louis, MO 63104, United States.
authors: William S M
authors: WS
authors: Wold
mesh_terms: D000256:Adenoviridae / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D000257:Adenoviridae Infections / Q000662:veterinary*; D000820:Animal Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D000818:Animals; D019295:Computational Biology; D006224:Cricetinae; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000378:metabolism* / Q000821:virology*; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D000949:Histocompatibility Antigens Class II
keywords: Adenovirus; Antiviral response; Innate immunity; Liver; Microarray; Syrian hamster; Transcriptome