title: Synthetic peptides for the immunodiagnosis of hepatitis A virus infection.
abstract: VP1, VP2 and VP3 molecules of hepatitis A virus are exposed capsid proteins that have shown to be antigenic and are used for diagnosis in recombinant-antigen commercial kits. In this study, we developed a sequence analysis in order to predict diagnostic peptide epitopes, followed by their spot synthesis on functionalized cellulose paper (Pepscan). This paper with synthetic peptides was tested against a sera pool of hepatitis A patients. Two peptide sequences, that have shown an antigenic recognition, were selected for greater scale synthesis on resin. A dimeric form of one of these peptides (IMT-1996), located in the C-Terminus region of protein VP1, was antigenic with a recognition frequency of 87-100% of anti-IgG antibodies and 100% of anti-IgM antibodies employing the immunological assays MABA and ELISA. We propose peptide IMT-1996, with less than twenty residues, as a cheaper alternative for prevalence studies and diagnosis of hepatitis A infection.
authors: Sección de Biohelmintiasis, Instituto de Medicina Tropical, Facultad de Medicina, UCV, Caracas, Venezuela. Electronic address: adrygauna@gmail.com.
authors: A
authors: A
authors: Gauna
authors: Sección de Biohelmintiasis, Instituto de Medicina Tropical, Facultad de Medicina, UCV, Caracas, Venezuela. Electronic address: salosada@yahoo.com.
authors: S
authors: S
authors: Losada
authors: Sección de Biohelmintiasis, Instituto de Medicina Tropical, Facultad de Medicina, UCV, Caracas, Venezuela. Electronic address: angelita.lorenzo@gmail.com.
authors: M
authors: M
authors: Lorenzo
authors: Sección de Biohelmintiasis, Instituto de Medicina Tropical, Facultad de Medicina, UCV, Caracas, Venezuela. Electronic address: henrybermudez2001@yahoo.com.
authors: H
authors: H
authors: Bermúdez
authors: Cátedra de Parasitología, Escuela de Medicina "Luis Razetti", Universidad Central de Venezuela, Venezuela. Electronic address: marilyan@gmail.com.
authors: M
authors: M
authors: Toledo
authors: Departamento de Virología, Instituto Nacional de Higiene-INH "Rafael Rangel", Venezuela. Electronic address: hperez113@hotmail.com.
authors: H
authors: H
authors: Pérez
authors: Departamento de Virología, Instituto Nacional de Higiene-INH "Rafael Rangel", Venezuela. Electronic address: elsach7@hotmail.com.
authors: E
authors: E
authors: Chacón
authors: Sección de Biohelmintiasis, Instituto de Medicina Tropical, Facultad de Medicina, UCV, Caracas, Venezuela; Centro para Estudios Sobre Malaria, Instituto de Altos Estudios "Dr. Arnoldo Gabaldón" INH-Ministerio del Poder Popular para la Salud, Caracas, Venezuela. Electronic address: noyaoo@yahoo.com.
authors: O
authors: O
authors: Noya
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D036022:Capsid Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006506:Hepatitis A / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000276:immunology; D012680:Sensitivity and Specificity; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015415:Biomarkers; D036022:Capsid Proteins; D011994:Recombinant Proteins; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins
keywords: Hepatitis A; Immunodiagnosis; MABA; Peptides; Spot synthesis; Virus