title: GlycoMob: an ion mobility-mass spectrometry collision cross section database for glycomics.
abstract: Ion mobility mass spectrometry (IM-MS) is a promising analytical technique for glycomics that separates glycan ions based on their collision cross section (CCS) and provides glycan precursor and fragment masses. It has been shown that isomeric oligosaccharide species can be separated by IM and identified on basis of their CCS and fragmentation. These results indicate that adding CCSs information for glycans and glycan fragments to searchable databases and analysis pipelines will increase identification confidence and accuracy. We have developed a freely accessible database, GlycoMob ( http://www.glycomob.org ), containing over 900 CCSs values of glycans, oligosaccharide standards and their fragments that will be continually updated. We have measured the absolute CCSs of calibration standards, biologically derived and synthetic N-glycans ionized with various adducts in positive and negative mode or as protonated (positive ion) and deprotonated (negative ion) ions.
authors: Department of Chemistry, University of Oxford, Oxford, OX1 3QZ, UK. weston.struwe@chem.ox.ac.uk.
authors: Weston B
authors: WB
authors: Struwe
authors: Free University Chemistry, Institute of Chemistry and Biochemistry, Takustrasse 3, 14195, Berlin, Germany.|Department of Molecular Physics, Fritz Haber Institute of the Max Planck Society, 14195, Berlin, Germany.
authors: Kevin
authors: K
authors: Pagel
authors: Department of Chemistry, University of Oxford, Oxford, OX1 3QZ, UK.
authors: Justin L P
authors: JL
authors: Benesch
authors: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
authors: David J
authors: DJ
authors: Harvey
authors: Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW 2109, Australia. matthew.campbell@mq.edu.au.
authors: Matthew P
authors: 0000-0002-9525-792X
authors: MP
authors: Campbell
mesh_terms: D062126:Databases, Chemical; D054794:Glycomics / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D011134:Polysaccharides / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D012984:Software
chemical_list: D011134:Polysaccharides
keywords: Database; Glycan; Glycoinformatics; Glycomics; Ion mobility; Mass spectrometry