title: Splicing-Dependent Trans-synaptic SALM3-LAR-RPTP Interactions Regulate Excitatory Synapse Development and Locomotion.
abstract: Synaptic adhesion molecules regulate diverse aspects of synapse development and plasticity. SALM3 is a PSD-95-interacting synaptic adhesion molecule known to induce presynaptic differentiation in contacting axons, but little is known about its presynaptic receptors and in vivo functions. Here, we identify an interaction between SALM3 and LAR family receptor protein tyrosine phosphatases (LAR-RPTPs) that requires the mini-exon B splice insert in LAR-RPTPs. In addition, SALM3-dependent presynaptic differentiation requires all three types of LAR-RPTPs. SALM3 mutant (Salm3(-/-)) mice display markedly reduced excitatory synapse number but normal synaptic plasticity in the hippocampal CA1 region. Salm3(-/-) mice exhibit hypoactivity in both novel and familiar environments but perform normally in learning and memory tests administered. These results suggest that SALM3 regulates excitatory synapse development and locomotion behavior.
authors: Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors: Yan
authors: Y
authors: Li
authors: Brain Research Centre and Department of Psychiatry, University of British Columbia, Vancouver, BC V6T 2B5, Canada.
authors: Peng
authors: P
authors: Zhang
authors: Department of Physiology, College of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul 110-749, Republic of Korea.
authors: Tae-Yong
authors: TY
authors: Choi
authors: Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of Korea.
authors: Sook Kyung
authors: SK
authors: Park
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors: Hanwool
authors: H
authors: Park
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors: Eun-Jae
authors: EJ
authors: Lee
authors: Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea.
authors: Dongsoo
authors: D
authors: Lee
authors: Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea.
authors: Junyeop Daniel
authors: JD
authors: Roh
authors: Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of Korea.
authors: Won
authors: W
authors: Mah
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors: Ryunhee
authors: R
authors: Kim
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors: Yangsik
authors: Y
authors: Kim
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors: Harah
authors: H
authors: Kwon
authors: Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of Korea.
authors: Yong Chul
authors: YC
authors: Bae
authors: Department of Physiology, College of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul 110-749, Republic of Korea.
authors: Se-Young
authors: SY
authors: Choi
authors: Brain Research Centre and Department of Psychiatry, University of British Columbia, Vancouver, BC V6T 2B5, Canada.
authors: Ann Marie
authors: AM
authors: Craig
authors: Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea. Electronic address: kime@kaist.ac.kr.
authors: Eunjoon
authors: E
authors: Kim
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002454:Cell Differentiation; D019706:Excitatory Postsynaptic Potentials; D005091:Exons; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007858:Learning; D008124:Locomotion; D008562:Membrane Glycoproteins; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules / Q000502:physiology*; D009473:Neuronal Plasticity; D020033:Protein Isoforms / Q000502:physiology; D011597:Psychomotor Performance; D022821:RNA Splice Sites; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: C000608522:Lrfn4 protein, mouse; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms; D022821:RNA Splice Sites; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2