title: Time-dependent viscometry study of endoglucanase action on xyloglucan: A real-time approach.
abstract: Hydrolysis of xyloglucan from Tamarindus indica and Hymenaea courbaril seeds with endoglucanase (EGII), which randomly breaks the (1→4)-linked β-glycosidic bonds of the polymer chain, was monitored in real time using time-dependent viscometry analysis (TDV). For both samples there was a decrease in the intrinsic viscosity ([η]), viscosity average molar mass (Mv), radius de gyration (Rg) and persistence length (Lp) immediately after the addition of the enzyme. It was observed the formation of oligosaccharides and oligomers composed of ∼2 units, up to 140min. Galactose-containing side chains two positions away from the non-substituted glucose, modulated the action of EGII, and the complete hydrolysis of the XG oligomers occurred after 24h. The results demonstrate for the first time the real-time degradation of xyloglucan as well the macromolecular and oligosaccharide composition during the EGII hydrolysis process.
authors: BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil.
authors: Vivian Cristina
authors: VC
authors: Spier
authors: BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil.
authors: Maria Rita
authors: MR
authors: Sierakowski
authors: BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil.
authors: Amid Tony
authors: AT
authors: Ibrahim
authors: BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil; Laboratory of Plant Carbohydrates, Department of Biochemistry, Federal University of Paraná, 81531-990 Curitiba, PR, Brazil.
authors: Jéssica C
authors: JC
authors: Scholze Baum
authors: Laboratory of Plant Carbohydrates, Department of Biochemistry, Federal University of Paraná, 81531-990 Curitiba, PR, Brazil.
authors: Joana Lea M
authors: JL
authors: Silveira
authors: BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil. Electronic address: rilton@ufpr.br.
authors: Rilton Alves
authors: RA
authors: de Freitas
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002845:Chromatography; D005936:Glucans / Q000737:chemistry*; D006868:Hydrolysis; D013997:Time Factors; D014783:Viscosity; D014990:Xylans / Q000737:chemistry*
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002480:Cellulase
keywords: Endoglucanase; Time-dependent viscometry; Xyloglucan