title: Survival dynamics of cystic fibrosis-related Gram-negative bacterial pathogens (Pseudomonas aeruginosa and Burkholderia cenocepacia) in Dead Sea and Atlantic Ocean waters.
abstract: Clinical cystic fibrosis (CF) Pseudomonas aeruginosa (n=6) and Burkholderia cenocepacia (n=4) were inoculated in marine brines from the Dead Sea and the Atlantic Ocean and their survival was monitored over a 1 month duration. In Dead Sea samples, all P. aeruginosa and B. cenocepacia isolates were non-detectable by culture following 24 h incubation, including the non-selective enrichment samples. In the Atlantic Ocean brine, over a 1 month period, mean P. aeruginosa counts decreased by only 0.25 log10 units and mean B. cenocepacia counts decreased by approximately 4 log10 units (10,000 cfu/ml). This study demonstrated that Dead Sea brine exerted a lethal effect within 24 h on planktonic P. aeruginosa and B. cenocepacia. Thus, the Dead Sea effectively purges these organisms from its environment on a daily basis.
authors: Pulmonology Institute and CF Center, Carmel Medical Center, Haifa, Israel and The B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel; Centre for Infection and Immunity (CII), School of Medicine, Dentistry and Biomedical Sciences, Queen's University, 97 Lisburn Road, Belfast BT9 7BL, UK.
authors: Michal
authors: M
authors: Shteinberg
authors: Institute of Evolution, University of Haifa, 199 Aba-Hushi Avenue, Mount Carmel, Haifa 3498838, Israel.
authors: Tamar
authors: T
authors: Kis-Papo
authors: Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, UK E-mail: j.moore@qub.ac.uk.
authors: Beverley C
authors: BC
authors: Millar
authors: Northern Ireland Regional Adult Cystic Fibrosis Centre, Level 8, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, UK.
authors: Jacqueline C
authors: JC
authors: Rendall
authors: Northern Ireland Regional Adult Cystic Fibrosis Centre, Level 8, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, UK.
authors: Damian G
authors: DG
authors: Downey
authors: Centre for Infection and Immunity (CII), School of Medicine, Dentistry and Biomedical Sciences, Queen's University, 97 Lisburn Road, Belfast BT9 7BL, UK; Northern Ireland Regional Adult Cystic Fibrosis Centre, Level 8, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, UK.
authors: J Stuart
authors: JS
authors: Elborn
authors: Centre for Infection and Immunity (CII), School of Medicine, Dentistry and Biomedical Sciences, Queen's University, 97 Lisburn Road, Belfast BT9 7BL, UK; Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, UK E-mail: j.moore@qub.ac.uk; Northern Ireland Regional Adult Cystic Fibrosis Centre, Level 8, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, UK; School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, UK.
authors: John E
authors: JE
authors: Moore
mesh_terms: D057508:Burkholderia cenocepacia / Q000502:physiology*; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability; D009792:Oceans and Seas; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology