title: Progesterone secretion by ovine granulosa cells: effects of nitric oxide and plane of nutrition.
abstract: The aim was to evaluate the effects of nutritional plane on in vitro progesterone (P4) secretion by granulosa (G) cells cultured in the presence or absence of effectors of the nitric oxide (NO) system. Ewes were randomly assigned into three nutritional groups: control (C), overfed (O; 2 × C), or underfed (U; 0.6 × C). Follicular development was induced by FSH injections. On day 15 of the estrous cycle, G cells were isolated and cultured with or without DETA-NONOate (NO donor), L-NAME (NO synthase [S] inhibitor), Arg and (or) LH for 8 h. DETA-NONOate decreased basal and LH-stimulated P4 secretion, and L-NAME increased basal P4 secretion in all groups. In U, Arg decreased LH-stimulated P4 secretion. These data demonstrate that (i) plane of nutrition affects basal P4 secretion by G cells, (ii) the NO donor decreases, NOS inhibitor increases but Arg does not affect basal P4 secretion, and (iii) effects of Arg on LH-stimulated P4 secretion are affected by plane of nutrition in FSH-treated sheep. Thus, plane of nutrition affects G cell function, and the NO system is involved in the regulation of basal and LH-stimulated P4 secretion. The mechanism of the NO system effects on secretory activity of G cells remains to be elucidated.
authors: a Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.
authors: Anna T
authors: AT
authors: Grazul-Bilska
authors: a Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.
authors: Casie S
authors: CS
authors: Bass
authors: a Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.
authors: Samantha L
authors: SL
authors: Kaminski
authors: b Department of Animal Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: George A
authors: GA
authors: Perry
authors: a Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.
authors: Dale A
authors: DA
authors: Redmer
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009752:Nutritional Status / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism*; D012756:Sheep
chemical_list: D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D011374:Progesterone
keywords: cellules de la granulosa; granulosa cells; in vitro; mouton; nitric oxide; nutrition; oxyde nitrique; progesterone; progestérone; sheep