title: Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts.
abstract: Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC50 values for DPPH scavenging were in Lyngbya water (LW, 18.78 ± 1.57 mg·mg(-1) DPPH) and Lyngbya methanol (LM, 59.56 ± 37.38 mg·mg(-1) DPPH) extracts. Iso-bolographic analysis revealed most of the combinations of extracts were antagonistic to each other, although LM-Spirulina methanol (SM) 1:1 had the highest synergistic rate of 86.65%. In vitro digestion studies showed that DPPH scavenging activity was considerably decreased in all extracts except for Pseudanabaena methanol (PM) and LM after the simulated digestion. All of the extracts were effective in reducing the calcium oxalate crystal size by nearly 60%-65% compared to negative control, while PM and Spirulina water (SW) extracts could inhibit both nucleation and aggregation of calcium oxalate by nearly 60%-80%.
authors: Salt and Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. paliwalchetan@gmail.com.|Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. paliwalchetan@gmail.com.
authors: Chetan
authors: C
authors: Paliwal
authors: Salt and Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. forghosh@gmail.com.|Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. forghosh@gmail.com.
authors: Tonmoy
authors: T
authors: Ghosh
authors: Salt and Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. khushboobhayani@gmail.com.
authors: Khushbu
authors: K
authors: Bhayani
authors: Salt and Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. maurya.micro@gmail.com.|Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. maurya.micro@gmail.com.
authors: Rahulkumar
authors: R
authors: Maurya
authors: Salt and Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. smishra@csmcri.org.|Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. smishra@csmcri.org.
authors: Sandhya
authors: S
authors: Mishra
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002129:Calcium Oxalate / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry; D000458:Cyanobacteria / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D052979:Phycobiliproteins / Q000737:chemistry; D010860:Pigments, Biological / Q000737:chemistry*; D053545:Spirulina / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052979:Phycobiliproteins; D010860:Pigments, Biological; D014867:Water; D002129:Calcium Oxalate; D002338:Carotenoids; D000432:Methanol
keywords: Antioxidants; Carotenoids; DPPH; calcium oxalate crystallization; in vitro digestibility; iso-bolographic analysis; phycobiliproteins