title: Dichloroacetate Decreases Cell Health and Activates Oxidative Stress Defense Pathways in Rat Alveolar Type II Pneumocytes.
abstract: Dichloroacetate (DCA) is a water purification byproduct that is known to be hepatotoxic and hepatocarcinogenic and to induce peripheral neuropathy and damage macrophages. This study characterizes the effects of the haloacetate on lung cells by exposing rat alveolar type II (L2) cells to 0-24 mM DCA for 6-24 hours. Increasing DCA concentration and the combination of increasing DCA concentration plus longer exposures decrease measures of cellular health. Length of exposure has no effect on oxidative stress biomarkers, glutathione, SOD, or CAT. Increasing DCA concentration alone does not affect total glutathione or its redox ratio but does increase activity in the SOD/CAT oxidative stress defense pathway. These data suggest that alveolar type II cells rely on SOD and CAT more than glutathione to combat DCA-induced stress.
authors: Biology Department, Davidson College, Davidson, NC 28035, USA.
authors: Alexis
authors: A
authors: Valauri-Orton
authors: Biology Department, Davidson College, Davidson, NC 28035, USA.
authors: Frizzi
authors: F
authors: Bschorer
authors: Biology Department, Davidson College, Davidson, NC 28035, USA.
authors: Karen K
authors: KK
authors: Bernd
mesh_terms: D056809:Alveolar Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D003999:Dichloroacetic Acid / Q000737:chemistry / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1
chemical_list: D003999:Dichloroacetic Acid; D002374:Catalase; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D005978:Glutathione