title: Sensitivity Enhancement in Solid-State Separated Local Field NMR Experiments by the Use of Adiabatic Cross-Polarization.
abstract: Measurement of dipolar couplings using separated local field (SLF) NMR experiment is a powerful tool for structural and dynamics studies of oriented molecules such as liquid crystals and membrane proteins in aligned lipid bilayers. Enhancing the sensitivity of such SLF techniques is of significant importance in present-day solid-state NMR methodology. The present study considers the use of adiabatic cross-polarization for this purpose, which is applied for the first time to one of the well-known SLF techniques, namely, polarization inversion spin exchange at the magic angle (PISEMA). The experiments have been carried out on a single crystal of a model peptide, and a dramatic enhancement in signal-to-noise up to 90% has been demonstrated.
authors: †Department of Physics and ‡NMR Research Centre, Indian Institute of Science, Bangalore 560012, India.
authors: Nitin P
authors: NP
authors: Lobo
authors: †Department of Physics and ‡NMR Research Centre, Indian Institute of Science, Bangalore 560012, India.
authors: Krishna V
authors: KV
authors: Ramanathan
keywords: ADRF; PISEMA; SLF; adiabatic CP; oriented systems; sensitivity enhancement