title: Electrical nanowell diagnostics sensors for rapid and ultrasensitive detection of prostate-specific antigen.
abstract: OBJECTIVE
  Alumina nanowell based disposable diagnostic biosensor for detecting and quantifying levels of prostate-specific antigen (PSA) from human serum has been designed, fabricated and tested.
  
  
  METHODS
  The biosensors were designed by integrating nanoporous alumina membranes onto printed circuit board platforms, resulting in the generation of high-density nanowell arrays with gold base electrodes. The size and density of the nanowells were leveraged toward achieving sieving action for size-based exclusion of nonspecific molecules and size-based confinement of the target PSA molecules.
  
  
  CONCLUSIONS
  We demonstrated PSA detection between 0.01 and 1000 ng/ml and detection and quantification of PSA from a 17 patient cohort validated using the Beckman Access system with >95% correlation.
authors: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors: Anjan
authors: A
authors: Panneer Selvam
authors: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors: Shalini
authors: S
authors: Prasad
authors: Department of Veterans Affairs Portland, Oregon Health Sciences University, OR, USA.
authors: Thomas W
authors: TW
authors: Barrett
authors: Department of Pathology, Oregon Health & Science University, Portland, OR, USA.
authors: Steven C
authors: SC
authors: Kazmierczak
mesh_terms: D015374:Biosensing Techniques; D006801:Humans; D057230:Limit of Detection; D008297:Male; D036103:Nanotechnology; D017430:Prostate-Specific Antigen / Q000032:analysis*
chemical_list: D017430:Prostate-Specific Antigen
keywords: alumina nanowells; charge screening; electrochemical impedance spectroscopy; nanowell biosensor diagnostics; prostate-specific antigen