title: In vitro fructose exposure overactivates NADPH oxidase and causes oxidative stress in the isolated rat aorta.
abstract: Fructose acutely interferes with cardiovascular function in humans and in animals, but the mechanisms remain unclear. Thus, we tested whether fructose can affect endothelial function without the interference of its metabolic effect by exposing the rat aorta to a high fructose concentration and then evaluate the vascular responses to vasoactive agents. We observed that fructose exposure causes overactivation of NADPH oxidase, which enhances superoxide anion production and increases NO degradation. Additionally, the enhanced vasoconstrictor action of hydrogen peroxide might exacerbate contractile responses. This vasoactive imbalance might be the key role by which fructose induces vascular dysfunction.
authors: Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil.
authors: Camila C P
authors: CC
authors: Almenara
authors: Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil; Post Graduate Program of Collective Health, Federal University of Espírito Santo, Av Marechal Campos 1468, Maruipe, 29042-755 Vitória, ES, Brazil.
authors: José G
authors: JG
authors: Mill
authors: Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil.
authors: Dalton V
authors: DV
authors: Vassallo
authors: Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil.
authors: Marcelo P
authors: MP
authors: Baldo
authors: Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Espírito Santo, Brazil. Electronic address: ale_padilha@hotmail.com.
authors: Alessandra S
authors: AS
authors: Padilha
mesh_terms: D000098:Acetophenones / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects*; D005632:Fructose / Q000633:toxicity*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D000098:Acetophenones; D005632:Fructose; D008353:Mannitol; C056165:acetovanillone; D002374:Catalase; D013482:Superoxide Dismutase; D019255:NADPH Oxidases
keywords: Endothelial dysfunction; Fructose; NADPH oxidase; Oxidative stress