title: Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis.
abstract: An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.
authors: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Yutaka
authors: Y
authors: Konya
authors: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Takeshi
authors: T
authors: Bamba
authors: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: fukusaki@bio.eng.osaka-u.ac.jp.
authors: Eiichiro
authors: E
authors: Fukusaki
mesh_terms: D000097:Acetonitriles; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D013237:Stereoisomerism; D013997:Time Factors; D014269:Trifluoroacetic Acid; D014867:Water
chemical_list: D000097:Acetonitriles; D000596:Amino Acids; D014867:Water; D014269:Trifluoroacetic Acid; C032159:acetonitrile
keywords: Chiral liquid chromatography; Time of flight mass spectrometry; Underivatized amino acids; d-Amino acids