title: A Study on Spectro-Analytical Aspects, DNA - Interaction, Photo-Cleavage, Radical Scavenging, Cytotoxic Activities, Antibacterial and Docking Properties of 3 - (1 - (6 - methoxybenzo [d] thiazol - 2 - ylimino) ethyl) - 6 - methyl - 3H - pyran - 2, 4 - dione and its Metal Complexes.
abstract: The focus of the present work is on the design, synthesis, characterization, DNA-interaction, photo-cleavage, radical scavenging, in-vitro cytotoxicity, antimicrobial, docking and kinetic studies of Cu (II), Cd (II), Ce (IV) and Zr (IV) metal complexes of an imine derivative, 3 - (1 - (6 - methoxybenzo [d] thiazol - 2 - ylimino) ethyl) - 6 - methyl - 3H - pyran - 2, 4 - dione. The investigation of metal ligand interactions for the determination of composition of metal complexes, corresponding kinetic studies and antioxidant activity in solution was carried out by spectrophotometric methods. The synthesized metal complexes were characterized by EDX analysis, Mass, IR, (1)H-NMR, (13)C-NMR and UV-Visible spectra. DNA binding studies of metal complexes with Calf thymus (CT) DNA were carried out at room temperature by employing UV-Vis electron absorption, fluorescence emission and viscosity measurement techniques. The results revealed that these complexes interact with DNA through intercalation. The results of in vitro antibacterial studies showed the enhanced activity of chelating agent in metal chelated form and thus inferring scope for further development of new therapeutic drugs. Cell viability experiments indicated that all complexes showed significant dose dependent cytotoxicity in selected cell lines. The molecular modeling and docking studies were carried out with energy minimized structures of metal complexes to identify the receptor to metal interactions.
authors: Department of Chemistry, Osmania University, Hyderabad, 500 007, Telangana, India.
authors: Mudavath
authors: M
authors: Ravi
authors: Department of Chemistry, Osmania University, Hyderabad, 500 007, Telangana, India.
authors: Kishan Prasad
authors: KP
authors: Chennam
authors: Department of Chemistry, Osmania University, Hyderabad, 500 007, Telangana, India.
authors: B
authors: B
authors: Ushaiah
authors: Department of Bio-Chemistry, Osmania University, Hyderabad, 500 007, Telangana, India.
authors: Ravi Kumar
authors: RK
authors: Eslavath
authors: Department of Bio-Chemistry, Osmania University, Hyderabad, 500 007, Telangana, India.
authors: Shyam
authors: S
authors: Perugu
authors: National Institute of Nutrition, Hyderabad, 500 007, Telangana, India.
authors: Rajanna
authors: R
authors: Ajumeera
authors: Department of Chemistry, Osmania University, Hyderabad, 500 007, Telangana, India. dr_saraladevich@yahoo.com.
authors: Ch Sarala
authors: ChS
authors: Devi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D062105:Molecular Docking Simulation; D055668:Photochemical Processes; D011487:Protein Conformation; D011714:Pyrans / Q000737:chemistry*; D013057:Spectrum Analysis; D014783:Viscosity; D019061:src-Family Kinases / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D056831:Coordination Complexes; D016166:Free Radical Scavengers; D011714:Pyrans; D004247:DNA; C427203:calf thymus DNA; D019061:src-Family Kinases
keywords: Antioxidant; Cytotoxicity; DNA binding; Photo cleavage