title: Systemic and local regulation of phosphate and nitrogen transporter genes by arbuscular mycorrhizal fungi in roots of winter wheat (Triticum aestivum L.).
abstract: Previous studies have reported that the expression of phosphate (Pi) or nitrogen (N) transporter genes in roots of plants could be regulated by arbuscular mycorrhizal (AM) fungi, but little is known whether the regulation is systemic or not. The present study investigated the systemic and local regulation of multiple phosphate and nitrogen transporter genes by four AM fungal species belonging to four genera in the roots of winter wheat. A split-root culture system with AM inoculated (MR) and non-inoculated root compartments (NR) was used to investigate the systemic or local responses of phosphate and nitrogen transporter genes to colonization by four AM fungi in the roots of wheat. The expression of four Pi transporter, five nitrate transporter, and three ammonium transporter genes was quantified using real-time PCR. Of the four AM fungi tested, all locally increased expression of the AM-inducible Pi transporter genes, and most locally decreased expression of a Pi-starvation inducible Pi transporter gene. The addition of N in soil increased the expression of either Pi starvation inducible Pi transporters or AM inducible Pi transporters. Inoculation with AM fungi either had no effect, or could locally or systemically down-regulate expression of nitrogen transporter genes depending on gene type and AM fungal species.
authors: Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Jianfeng
authors: J
authors: Duan
authors: Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: tianh@nwsuaf.edu.cn.
authors: Hui
authors: H
authors: Tian
authors: Department of Agronomy and Horticulture, University of Nebraska-Lincoln, 316 Keim Hall, Lincoln, NE 68503-0915, USA.
authors: Rhae A
authors: RA
authors: Drijber
authors: Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Yajun
authors: Y
authors: Gao
mesh_terms: D002352:Carrier Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant; D038821:Mycorrhizae / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D002352:Carrier Proteins; D028061:Phosphate Transport Proteins; D009584:Nitrogen
keywords: Arbuscular mycorrhizal fungi; Nitrogen; Phosphorus; Transporter gene; Wheat