title: Combining microscopy with mesoscopy using optical and optoacoustic label-free modes.
abstract: Biology requires observations at multiple geometrical scales, a feature that is not typically offered by a single imaging modality. We developed a hybrid optical system that not only provides different contrast modes but also offers imaging at different geometrical scales, achieving uniquely broad resolution and a 1000-fold volume sampling increase compared to volumes scanned by optical microscopy. The system combines optoacoustic mesoscopy, optoacoustic microscopy and two-photon microscopy, the latter integrating second and third harmonic generation modes. Label-free imaging of a mouse ear and zebrafish larva ex-vivo demonstrates the contrast and scale complementarity provided by the hybrid system. We showcase the superior anatomical orientation offered by the label-free capacity and hybrid operation, over fluorescence microscopy, and the dynamic selection between field of view and resolution achieved, leading to new possibilities in biological visualization.
authors: Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany; Chair for Biological Imaging, Technische Universität München, Ismaningerstr. 22, 81675 München, Germany.
authors: Dominik
authors: D
authors: Soliman
authors: Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany; Chair for Biological Imaging, Technische Universität München, Ismaningerstr. 22, 81675 München, Germany.
authors: George J
authors: GJ
authors: Tserevelakis
authors: Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany; Chair for Biological Imaging, Technische Universität München, Ismaningerstr. 22, 81675 München, Germany.
authors: Murad
authors: M
authors: Omar
authors: Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany; Chair for Biological Imaging, Technische Universität München, Ismaningerstr. 22, 81675 München, Germany.
authors: Vasilis
authors: V
authors: Ntziachristos
mesh_terms: D000818:Animals; D003287:Contrast Media; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D064847:Multimodal Imaging / Q000295:instrumentation* / Q000379:methods; D061088:Photoacoustic Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods; D015027:Zebrafish
chemical_list: D003287:Contrast Media