title: CyPA-CD147-ERK1/2-cyclin D2 signaling pathway is upregulated during rat left ventricular hypertrophy.
abstract: The changes of serum cyclophilin A (CyPA), its receptor CD147 and the downstream signaling pathway during the process of cardiac hypertrophy remain unknown. The present study aims to investigate the relationships between CyPA-CD147-ERK1/2-cyclin D2 signaling pathway and the development of cardiac hypertrophy. Left ventricular hypertrophy was prepared by 2-kidney, 2-clip in Sprague-Dawley rats and observed for 1 week, 4 and 8 weeks. Left ventricular hypertrophy was evaluated by ratio of left ventricular heart weight to body weight (LVW/BW) and cardiomyocyte cross sectional area (CSA). CyPA levels in serum were determined with a rat CyPA ELISA kit. Expressions of CyPA, CD147, phospho-ERK1/2 and cyclin D2 in left ventricular myocytes were determined by Western blot and immunostaining. Compared with sham groups, systolic blood pressure reached hypertensive levels at 4 weeks in 2K2C groups. LVW/BW and CSA in 2K2C groups were significantly increased at 4 and 8 weeks after clipping. ELISA results indicated a prominent increase in serum CyPA level associated with the degree of left ventricular hypertrophy. Western blot revealed that the expressions of CyPA, CD147, phospho-ERK1/2 and cyclin D2 in left ventricular tissues were also remarkably increased as the cardiac hypertrophy developed. The results of the present study demonstrates that serum CyPA and CyPA-CD147-ERK1/2-cyclin D2 signaling pathway in ventricular tissues are time-dependently upregulated and activated with the process of left ventricular hypertrophy. These data suggest that CyPA-CD147 signaling cascade might play a role in the pathogenesis of left ventricular hypertrophy, and CyPA might be a prognosticator of the degree of left ventricular hypertrophy.
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.|The First Clinical Medical College, Guangzhou Medical University, Guangzhou 510182, China.
authors: Fu-Cai
authors: FC
authors: Tang
authors: Department of Pathology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
authors: Hong-Yan
authors: HY
authors: Wang
authors: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
authors: Ming-Ming
authors: MM
authors: Ma
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.|The Second Clinical Medical College, Guangzhou Medical University, Guangzhou 510182, China.
authors: Tian-Wang
authors: TW
authors: Guan
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.|The Third Clinical Medical College, Guangzhou Medical University, Guangzhou 510182, China.
authors: Long
authors: L
authors: Pan
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.|The Third Clinical Medical College, Guangzhou Medical University, Guangzhou 510182, China.
authors: Dun-Chen
authors: DC
authors: Yao
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.|The First Clinical Medical College, Guangzhou Medical University, Guangzhou 510182, China.
authors: Ya-Lan
authors: YL
authors: Chen
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.|The Second Clinical Medical College, Guangzhou Medical University, Guangzhou 510182, China.
authors: Wei-Bei
authors: WB
authors: Chen
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China. tuys@gzhmu.edu.cn.
authors: Yong-Sheng
authors: YS
authors: Tu
authors: Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
authors: Xiao-Dong
authors: XD
authors: Fu
mesh_terms: D000818:Animals; D051926:Basigin / Q000378:metabolism; D001794:Blood Pressure; D056742:Cyclin D2; D021984:Cyclophilin A / Q000378:metabolism; D006973:Hypertension; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D032383:Myocytes, Cardiac; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: C486197:Ccnd2 protein, rat; D056742:Cyclin D2; D051926:Basigin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D021984:Cyclophilin A