title: Cytogenetic evaluation for the genotoxicity of bisphenol-A in Chinese hamster ovary cells.
abstract: Bisphenol A (BPA), identified as an endocrine disruptor, is an important man-made compound used in a wide range of consumer products. The MTT assay, comet assay, micronucleus test, chromosome aberration test, and Ames assay were conducted to assess the cytotoxic, genotoxic, cytogenetic effects, and mutagenic activity of BPA. After BPA exposure, we showed significant increases in cytotoxicity and level of DNA damage indicated by Olive tail moment, tail length, and % tail DNA in a similar dose- and time-dependent manner. Significant increases in micronucleus frequency and conventional chromosome aberrations were also observed after BPA treatment. The major types of structural aberrations were breaks, gaps, and fragments. However, no positive mutagenic activity of BPA was observed in any of the tester strains. Taken together, the data obtained in this study clearly demonstrated that BPA is not mutagenic but could exhibit significant genotoxic and cytogenetic effects in Chinese hamster ovary cells.
authors: School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, Suzhou 215123, China.
authors: Lili
authors: L
authors: Xin
authors: Experimental Center of Medical College, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Yao
authors: Y
authors: Lin
authors: Experimental Center of Medical College, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Aiqing
authors: A
authors: Wang
authors: School of Radiation Medicine and Protection, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Wei
authors: W
authors: Zhu
authors: Sanitation & Environment Technology Institute, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Yu
authors: Y
authors: Liang
authors: Sanitation & Environment Technology Institute, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Xiaojun
authors: X
authors: Su
authors: Experimental Center of Medical College, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Chengjiao
authors: C
authors: Hong
authors: Experimental Center of Medical College, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Jianmei
authors: J
authors: Wan
authors: Experimental Center of Medical College, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
authors: Yarong
authors: Y
authors: Wang
authors: School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, Suzhou 215123, China. Electronic address: hltian@suda.edu.cn.
authors: Hailin
authors: H
authors: Tian
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced* / Q000706:statistics & numerical data; D006224:Cricetinae; D003412:Cricetulus; D020732:Cytogenetic Analysis / Q000379:methods*; D004247:DNA / Q000187:drug effects*; D009152:Mutagenicity Tests; D010636:Phenols / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004247:DNA; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Cytogenetic; DNA damage; Endcorine disruptor; Micronucleus; Mutagenicity