title: The First Molecular Characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) Vitellogenin Expression Products and a Partial cDNA Sequence Isolation.
abstract: Anopheles (Nyssorhynchus) aquasalis Curry, 1932, is an anopheline that plays an important role in Plasmodium transmission in the New World. Few studies have explored the molecular reproductive biology of this vector species. In the current study, we isolated and sequenced a partial, 886 bp, cDNA fragment of a vitellogenin (Vtg) gene from An. aquasalis. The transcription profile of this Vtg gene in adult females was characterized, and it is similar to other members of the Culicidae. We also detected a smaller subunit of An. aquasalis Vtg proteins in ovary tissue and characterized its translational profile in previtellogenic and vitellogenic females. Our results represent the first molecular characterization of An. aquasalis vitellogenin expression products.
authors: Departamento de Parasitologia, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP 05508-000. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular - INCT-EM, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, 21.941-902. These authors contributed equally to this work. alcosta@icb.usp.br.
authors: André Luis
authors: AL
authors: Costa-Da-Silva
authors: Departamento de Parasitologia, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP 05508-000. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular - INCT-EM, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, 21.941-902. These authors contributed equally to this work.
authors: Laisa Sentieri
authors: LS
authors: Lorenti
authors: Departamento de Parasitologia, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP 05508-000. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular - INCT-EM, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, 21.941-902.
authors: Soraia
authors: S
authors: De Lima Oliveira
authors: Departamento de Parasitologia, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP 05508-000.
authors: André Franco
authors: AF
authors: Cardoso
authors: Departamento de Parasitologia, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP 05508-000. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular - INCT-EM, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, 21.941-902.
authors: Margareth Lara
authors: ML
authors: Capurro
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D014819:Vitellogenins
keywords: Anopheles aquasalis; mosquito; physiology; vitellogenesis; vitellogenin