title: Difference of microbial community stressed in artificial pit muds for Luzhou-flavour liquor brewing revealed by multiphase culture-independent technology.
abstract: OBJECTIVE
  Artificial pit muds (APMs) is produced by peats, aged pit muds, yellow and black clays etc. and is one of essential factors for Luzhou-flavour liquor production. The microbial community of APMs significantly influence the quality of Luzhou-flavour liquor. The aim of this study was to investigate the differences in bacterial, archaeal and fungal community of APMs, starters and materials.
  
  
  RESULTS
  Multiphase culture-independent technology were employed in this study, including nested PCR-denaturing gradient gel electrophoresis (nested PCR-DGGE), phospholipid fatty acid (PLFA), phospholipid ether lipids (PLEL) and fluorescence in situ hybridization (FISH) analysis. Results suggested that the microbial diversity significantly changed under environmental stress and different culture patterns during APMs cultivation. The dominant bacteria in APMs mainly fell into Clostridiales, Lactobacillales, Bacteroidales and Rhizobiales, Archaea affiliated with Methanomicrobiales and Methanosarcinales, and fungi belonged to Saccharomycetales and Eurotiales. Furthermore, the microbial community structures of APMs cultured by ground pile pattern were more similar with that of aged pit muds, meanwhile, the relative bands intensities of microbes, which are the main contributors for liquor brewing, increased with the culture times.
  
  
  CONCLUSIONS
  Not only the niche selection and biogeochemical properties of APMs, but also the mutual collaboration and constraint between different microbes may result in enriching different liquor-brewing microbes into APMs.
  
  
  CONCLUSIONS
  APM cultivation technology was necessary to promote enriching functional liquor-brewing microbes into APMs. These results may facilitate understanding the microbial succession during APMs manufacture.
authors: College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, China.|Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, China.
authors: L
authors: L
authors: Zhang
authors: College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, China.|Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, China.|National Engineering Research Centre of Solid-State Brewing, Luzhou, China.
authors: R
authors: R
authors: Zhou
authors: College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, China.|Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, China.
authors: M
authors: M
authors: Niu
authors: College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, China.|Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, China.
authors: J
authors: J
authors: Zheng
authors: College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, China.|Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, China.
authors: C
authors: C
authors: Wu
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology*; D000538:Aluminum Silicates / Q000737:chemistry*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D000077215:Clay; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction
chemical_list: D000538:Aluminum Silicates; D000077215:Clay
keywords: FISH analysis; PCR-DGGE; PLFA and PLEL analysis; artificial pit muds; microbial community