title: Infectious Bursal Disease Virus VP3 Upregulates VP1-Mediated RNA-Dependent RNA Replication.
abstract: Genome replication is a critical step in virus life cycles. Here, we analyzed the role of the infectious bursal disease virus (IBDV) VP3, a major component of IBDV ribonucleoprotein complexes, on the regulation of VP1, the virus-encoded RNA-dependent RNA polymerase (RdRp). Data show that VP3, as well as a peptide mimicking its C-terminal domain, efficiently stimulates the ability of VP1 to replicate synthetic single-stranded RNA templates containing the 3' untranslated regions (UTRs) from the IBDV genome segments.
authors: Centro Nacional de Biotecnología (CSIC), Cantoblanco, Madrid, Spain Institut de Biologia Molecular de Barcelona (CSIC), Parc Científic de Barcelona, Barcelona, Spain.
authors: Diego
authors: D
authors: Ferrero
authors: Centro Nacional de Biotecnología (CSIC), Cantoblanco, Madrid, Spain.
authors: Damià
authors: D
authors: Garriga
authors: MRC-University of Glasgow, Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors: Aitor
authors: A
authors: Navarro
authors: Centro Nacional de Biotecnología (CSIC), Cantoblanco, Madrid, Spain jfrodrig@cnb.csic.es nvmcri@ibmb.csic.es.
authors: José F
authors: JF
authors: Rodríguez
authors: Institut de Biologia Molecular de Barcelona (CSIC), Parc Científic de Barcelona, Barcelona, Spain jfrodrig@cnb.csic.es nvmcri@ibmb.csic.es.
authors: Núria
authors: N
authors: Verdaguer
mesh_terms: D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D007243:Infectious bursal disease virus / Q000235:genetics / Q000502:physiology*; D058105:Polymerization; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism* / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D012367:RNA, Viral; C107669:VP1 protein, infectious bursal disease virus; C478244:VP3 protein, infectious bursal disease virus; D015678:Viral Structural Proteins