title: Pilot investigation of the molecular discrimination of human osteoblasts from different bone entities.
abstract: In oral and maxillofacial surgery, autologous grafts from the iliac crest remain the 'gold standard' for alveolar ridge reconstruction, whereas intraoral bone grafts are considered in smaller defects. To date, a comparison of the osteogenic potential of osteoblasts with regard to their tissue origin is missing. Primary osteoblasts have proven useful for the investigation of the tissue-specific osteogenic properties. The present study compares primary human alveolar (aHOBs) and iliac osteoblasts (iHOBs) derived from three female patients undergoing routine intraoral bone grafting. Proliferation potential of the osteoblasts was evaluated using real-time impedance monitoring. Relative gene expression of bone specific biomarkers was analyzed and quantified using quantitative polymerase chain reactions (qPCR). Immunohistochemistry and phase contrast microscopy were performed, as well as alkaline phosphatase assay and alizarin red staining to visualize morphology and mineralization capacity. A twofold faster proliferation rate of aHOBs compared with iHOBs (130 h vs. 80 h) was observed. Alkaline phosphatase activity and alizarin red staining in both HOBs indicated similar mineralization capacity. Gene expression of seven genes (BMP1, CSF-1, TGFBR1, ICAM1, VCAM1, SPP1 and DLX5) was significantly higher in iHOB than in aHOB samples. These data suggest a higher osteogenic potential of osteoblasts derived from the iliac crest compared with primary osteoblasts from the alveolar bone and may lead to a better understanding of the molecular impact of bone cells from different bone entities on bone regeneration in alveolar ridge reconstructions.
authors: Department of Oral Biotechnology, University Medical Center Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany; Faculty of Biology, University of Freiburg, Schaenzlestraße 1, 79104 Freiburg, Germany. Electronic address: martin.wein@uniklinik-freiburg.de.
authors: Martin
authors: M
authors: Wein
authors: Department of Oral and Maxillofacial Surgery, University Medical Center Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.
authors: Tobias
authors: T
authors: Fretwurst
authors: Department of Oral- and Maxillofacial Surgery, Charité Campus Virchow, Augustenburger Platz 1, 13353 Berlin, Germany.
authors: Susanne
authors: S
authors: Nahles
authors: Department of Oral and Maxillofacial Surgery, University Medical Center Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.
authors: Fabian
authors: F
authors: Duttenhoefer
authors: Department of Oral Biotechnology, University Medical Center Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.
authors: Pascal
authors: P
authors: Tomakidi
authors: Department of Oral Biotechnology, University Medical Center Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.
authors: Thorsten
authors: T
authors: Steinberg
authors: Department of Oral and Maxillofacial Surgery, University Medical Center Freiburg, Hugstetter Str. 55, 79106 Freiburg, Germany.
authors: Katja
authors: K
authors: Nelson
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D000539:Alveolar Process / Q000166:cytology*; D015415:Biomarkers / Q000032:analysis; D055395:Bone Morphogenetic Protein 1 / Q000235:genetics; D001861:Bone Regeneration / Q000235:genetics; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D017097:Electric Impedance; D005260:Female; D015870:Gene Expression / Q000235:genetics; D018398:Homeodomain Proteins; D006801:Humans; D007085:Ilium / Q000166:cytology*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics; D008875:Middle Aged; D010006:Osteoblasts / Q000502:physiology*; D053495:Osteopontin / Q000235:genetics; D010865:Pilot Projects; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D014157:Transcription Factors; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D015415:Biomarkers; C100596:DLX5 protein, human; D018398:Homeodomain Proteins; C573420:ICAM1 protein, human; D018125:Receptors, Transforming Growth Factor beta; C506895:SPP1 protein, human; D014157:Transcription Factors; D019010:Vascular Cell Adhesion Molecule-1; D053495:Osteopontin; D018799:Intercellular Adhesion Molecule-1; D016173:Macrophage Colony-Stimulating Factor; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; C000625841:TGFBR1 protein, human; D000469:Alkaline Phosphatase; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1
keywords: Alveolar osteoblast; Gene expression; Iliac osteoblast; Mineralization; Osteogenic markers; Primary osteoblast; Proliferation