title: Effect of Matrix Metalloproteinase-inhibiting Solutions and Aging Methods on Dentin Bond Strength.
abstract: OBJECTIVE
  This study examined the effects of matrix metalloproteinase-inhibiting solutions and aging methods on the bond strength between resin composite and human dentin.
  
  
  METHODS
  Crown segments of 105 human non-carious molars were bonded using simulated pulpal pressure at 20 cm water pressure. The teeth were randomly split into 5 groups according to the solution applied: CG (control, no solution), CHX (0.2% chlorhexidine), EPE (10% ethanolic propolis extract), APE (aqueous propolis extract), and E (70% ethanol). Each solution was left on the acid-etched dentin for 1 min. Adper Single Bond 2 and resin composite (Filtek Z350 XT) were applied to all specimens. The 5 groups were subdivided according to the aging method: SI (sectioned immediately); S (storage in artificial saliva for 6 months); and T (thermomechanical aging with 240,000 mechanical cycles and 1000 thermal cycles). Specimens were sectioned into sticks and subjected to microtensile testing. Bond strength data were analyzed by two-factor ANOVA followed by a post-hoc Tukey's test (α=0.05).
  
  
  RESULTS
  For the factor "solution", there was no significant difference among the groups (p=0.32). For the factor "aging method", significant differences were found (p<0.001), with the following mean bond strengths (MPa): SI: 31.1; S: 24.4; T: 26.8.
  
  
  CONCLUSIONS
  The use of matrix metalloproteinase-inhibiting solutions on dentin as an adjunct to the application of an etch-and-rinse adhesive does not prevent the loss of bond strength after aging. Nevertheless, these solutions have no adverse effect on adhesion to tooth structure.
authors: Letícia C C Costa
authors: LC
authors: Perote
authors: Maria Beatriz Beber
authors: MB
authors: Kamozaki
authors: Natália C
authors: NC
authors: Gutierrez
authors: Franklin R
authors: FR
authors: Tay
authors: Cesar R
authors: CR
authors: Pucci
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D002710:Chlorhexidine / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry; D003764:Dental Materials / Q000737:chemistry*; D003804:Dentin / Q000648:ultrastructure*; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D011429:Propolis / Q000737:chemistry; D011897:Random Allocation; D012464:Saliva, Artificial / Q000737:chemistry; D013314:Stress, Mechanical; D013696:Temperature; D013718:Tensile Strength; D013997:Time Factors
chemical_list: C554641:Adper single bond 2; D003188:Composite Resins; D003738:Dental Cements; D003764:Dental Materials; D061965:Matrix Metalloproteinase Inhibitors; D010756:Phosphoric Acids; D012464:Saliva, Artificial; C533204:filtek Z350; D000431:Ethanol; D011429:Propolis; C030242:phosphoric acid; D002710:Chlorhexidine