title: Structural identification of putative USPs in Catharanthus roseus.
abstract: Nucleotide sequences of the C. roseus SRA database were assembled and translated in order to detect putative universal stress proteins (USPs). Based on the known conserved USPA domain, 24 Pfam putative USPA proteins in C. roseus were detected and arranged in six architectures. The USPA-like domain was detected in all architectures, while the protein kinase-like (or PK-like), (tyr)PK-like and/or U-box domains are shown downstream it. Three other domains were also shown to coexist with the USPA domain in C. roseus putative USPA sequences. These domains are tetratricopeptide repeat (or TPR), apolipophorin III (or apoLp-III) and Hsp90 co-chaperone Cdc37. Subsequent analysis divided USPA-like domains based on the ability to bind ATP. The multiple sequence alignment indicated the occurrence of eight C. roseus residues of known features of the bacterial 1MJH secondary structure. The data of the phylogenetic tree indicated several distinct groups of USPA-like domains confirming the presence of high level of sequence conservation between the plant and bacterial USPA-like sequences.
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: bahieldin55@gmail.com.
authors: Ahmed
authors: A
authors: Bahieldin
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: ahmed_atefaig2@yahoo.com.
authors: Ahmed
authors: A
authors: Atef
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt. Electronic address: ahmedshokry1@gmail.com.
authors: Ahmed M
authors: AM
authors: Shokry
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: Skarim@kau.edu.sa.
authors: Saleh
authors: S
authors: Al-Karim
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: sangtas@mit.edu.
authors: Sanaa G
authors: SG
authors: Al Attas
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt. Electronic address: nouromar71@yahoo.com.
authors: Nour O
authors: NO
authors: Gadallah
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt; Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), Faculty of Medicine, King Abdulaziz University (KAU), Jeddah, Saudi Arabia. Electronic address: sedris@aucegypt.edu.
authors: Sherif
authors: S
authors: Edris
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt. Electronic address: m_alkordy@yahoo.com.
authors: Magdy A
authors: MA
authors: Al-Kordy
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: aomar@kau.edu.sa.
authors: Abdulkader M Shaikh
authors: AM
authors: Omer
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: jsabir2622@gmail.com.
authors: Jamal S M
authors: JS
authors: Sabir
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt. Electronic address: ahmedramadan782@yahoo.com.
authors: Ahmed M
authors: AM
authors: Ramadan
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: ahajarr@gmail.com.
authors: Abdulrahman S M
authors: AS
authors: Al-Hajar
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia. Electronic address: rania_aryah@hotmail.co.uk.
authors: Rania M
authors: RM
authors: Makki
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: sabmahmoud@yahoo.com.
authors: Sabah M
authors: SM
authors: Hassan
authors: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: fm_domyati@hotmail.com.
authors: Fotouh M
authors: FM
authors: El-Domyati
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D030008:Catharanthus / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D030541:Databases, Genetic; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D000255:Adenosine Triphosphate
keywords: 1JMV; 1MJH; Domain; Universal stress proteins