title: In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.
abstract: Recently, induced pluripotent stem cells (iPSCs) have been generated in vivo from reprogrammable mice. These in vivo iPSCs display features of totipotency, i.e., they differentiate into the trophoblast lineage, as well as all 3 germ layers. Here, we developed a new reprogrammable mouse model carrying an Oct4-GFP reporter gene to facilitate the detection of reprogrammed pluripotent stem cells. Without doxycycline administration, some of the reprogrammable mice developed aggressively growing teratomas that contained Oct4-GFP(+) cells. These teratoma-derived in vivo PSCs were morphologically indistinguishable from ESCs, expressed pluripotency markers, and could differentiate into tissues of all 3 germ layers. However, these in vivo reprogrammed PSCs were more similar to in vitro iPSCs than ESCs and did not contribute to the trophectoderm of the blastocysts after aggregation with 8-cell embryos. Therefore, the ability to differentiate into the trophoblast lineage might not be a unique characteristic of in vivo iPSCs.
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Hyun Woo
authors: HW
authors: Choi
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Jong Soo
authors: JS
authors: Kim
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Yean Ju
authors: YJ
authors: Hong
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Hyuk
authors: H
authors: Song
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Han Geuk
authors: HG
authors: Seo
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.
authors: Jeong Tae
authors: JT
authors: Do
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D013724:Teratoma / Q000473:pathology*
chemical_list: D050814:Octamer Transcription Factor-3; D049452:Green Fluorescent Proteins