title: Evaluation of the diagnostic accuracy of nonstructural protein 1 Ag-based tests for dengue virus in Asian population: a meta-analysis.
abstract: BACKGROUND
  Nonstructural protein 1 (NS1) Ag-based tests are useful for detecting dengue virus (DENV), but there is lack of evidence on the diagnostic accuracy of NS1 Ag-based tests in Asian population. Thus, we conducted this meta-analysis to obtain the overall estimated and summarized performance of the NS1 Ag-based tests in the detection of DENV in Asia.
  
  
  METHODS
  PubMed, Embase and Medline were searched for studies that evaluated the diagnostic validity of NS1 Ag-based tests between January 1990 and November 2014. Data were analyzed by Meta-Disc and STATA software.
  
  
  RESULTS
  A total of 18 studies including 3342 dengue cases and 1904 control cases which fulfilled the inclusion criteria were considered for analysis. The pooled sensitivity and specificity for NS1 Ag-based tests was 66 % (95 % CI 64.5-67.5) and 97.9 % (95 % CI 97.3-100), respectively. STRIP has the overall highest sensitivity (72.9 %, 95 % CI 70.1-75.5). According to viral serotype, the test with the highest sensitivity for DENV1, DENV2 and DENV3 were Platelia (83.7 %, 95 % CI 79.7-87.1), Panbio (71.8 %, 95 % CI 65.5-80.9) and STRIP (81.9 %, 95 % CI 75.5-87.2) respectively. The highest sensitivity for primary infection was Platelia (95.1 %, 95 % CI 92.6-96.9) and for secondary infection was STRIP (64 %, 95 % CI 53.2-73.9).
  
  
  CONCLUSIONS
  Our meta-analysis suggests that NS1 Ag-based test is a good diagnostic method for DENV with a high specificity. However, viral serotype, serological status, clinical severity and the duration of illness are the main factors influencing the diagnostic accuracy.
authors: Department of Clinical Laboratory, Jin Hua Hospital, Zhejiang University, 351 Ming Yue Street, Jin Hua, 321000, , Zhejiang, China. Shanxy861@sina.com.
authors: Xiaoyun
authors: X
authors: Shan
authors: Department of Clinical Laboratory, Jin Hua Hospital, Zhejiang University, 351 Ming Yue Street, Jin Hua, 321000, , Zhejiang, China. 1712426411@qq.com.
authors: Xiangmei
authors: X
authors: Wang
authors: Department of Clinical Laboratory, Jin Hua Hospital, Zhejiang University, 351 Ming Yue Street, Jin Hua, 321000, , Zhejiang, China. yqq2517@126.com.
authors: Qing
authors: Q
authors: Yuan
authors: Department of Clinical Laboratory, Jin Hua Hospital, Zhejiang University, 351 Ming Yue Street, Jin Hua, 321000, , Zhejiang, China. 1923895570@qq.com.
authors: Yaping
authors: Y
authors: Zheng
authors: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qing ChunRoad, Hangzhou, 310003, , Zhejiang, China. zhhlgp@126.com.
authors: Honghe
authors: H
authors: Zhang
authors: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qing ChunRoad, Hangzhou, 310003, , Zhejiang, China. georgewuer@126.com.|Department of Public Health, Hangzhou Normal University School of Medicine, 16 Xuelin Street, Hangzhou, 310016, Zhejiang, China. georgewuer@126.com.
authors: Yihua
authors: Y
authors: Wu
authors: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qing ChunRoad, Hangzhou, 310003, , Zhejiang, China. junyanghzzju@163.com.|Department of Public Health, Hangzhou Normal University School of Medicine, 16 Xuelin Street, Hangzhou, 310016, Zhejiang, China. junyanghzzju@163.com.
authors: Jun
authors: J
authors: Yang
mesh_terms: D044466:Asian People; D003715:Dengue / Q000139:chemically induced / Q000453:epidemiology*; D003716:Dengue Virus / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009864:Ontario / Q000453:epidemiology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D065288:Serogroup; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins