title: Characterization and Isolation of Human T Cell Progenitors.
abstract: During their development, human T cells undergo similar genomic changes and pass through the same developmental checkpoints as developing thymocytes in the mouse. The difference between both species, however, is that some of these developmental stages are characterized by different phenotypic markers and as a result, evidence emerges that the molecular regulation of human T cell development subtly differs from the mouse [1-4]. In this chapter, we describe in detail how the different stages of human T cell development can be characterized and isolated using specific surface markers.
authors: The Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine and Health Sciences, Ghent University Hospital, Ghent University, De Pintelaan 185 Blok A-4, 9000, Ghent, Belgium.
authors: Inge
authors: I
authors: Van de Walle
authors: Karin
authors: K
authors: Davids
authors: Tom
authors: T
authors: Taghon
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008212:Lymphocyte Depletion; D054503:Lymphoid Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D060168:Thymocytes / Q000166:cytology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D016692:Receptors, Antigen, T-Cell, gamma-delta