title: Characterization of two endoglucanases for the classification of the earthworm, Eisenia fetida Waki.
abstract: Eisenia fetida and Eisenia andrei are vermicomposting species that are used as model animals for testing chemical material toxicology. Eisenia spp. are grown commercially in various fields in Japan. However, these two species have not been classified because it is difficult to distinguish them morphologically; thus, all bred earthworms are called E. fetida. However, it has been proposed that these two species have different expression regulation mechanisms. Here, we classified a sample of earthworms purchased from several farms, confirming that both E. fetida and E. andrei are present in Japanese earthworm breeding programs. We also characterized two highly active endoglucanases (EfEG1 and EfEG2) from the E. fetida Waki strain, which contained strong fibrinolytic enzymes for improving human health. We confirmed that EfEG1 is 1371 bp long and belongs to GHF9. Thus, E. fetida Waki may have commercial application for biomass utilization and as a dietary health supplement.
authors: a Department of Materials Engineering , National Institute of Technology, Nagaoka College , Nagaoka , Japan.
authors: Shin-ichi
authors: S
authors: Akazawa
authors: a Department of Materials Engineering , National Institute of Technology, Nagaoka College , Nagaoka , Japan.
authors: Yuki
authors: Y
authors: Ikarashi
authors: a Department of Materials Engineering , National Institute of Technology, Nagaoka College , Nagaoka , Japan.
authors: Jun
authors: J
authors: Yarimizu
authors: a Department of Materials Engineering , National Institute of Technology, Nagaoka College , Nagaoka , Japan.
authors: Keisuke
authors: K
authors: Yokoyama
authors: a Department of Materials Engineering , National Institute of Technology, Nagaoka College , Nagaoka , Japan.
authors: Tomoya
authors: T
authors: Kobayashi
authors: b Department of Bioengineering , Nagaoka University of Technology , Nagaoka , Japan.
authors: Hikaru
authors: H
authors: Nakazawa
authors: b Department of Bioengineering , Nagaoka University of Technology , Nagaoka , Japan.
authors: Wataru
authors: W
authors: Ogasawara
authors: b Department of Bioengineering , Nagaoka University of Technology , Nagaoka , Japan.
authors: Yasushi
authors: Y
authors: Morikawa
mesh_terms: D000818:Animals; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009835:Oligochaeta / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D014990:Xylans / Q000737:chemistry / Q000378:metabolism
chemical_list: D005343:Fibrinolytic Agents; D005936:Glucans; D007527:Isoenzymes; D011994:Recombinant Proteins; D014990:Xylans; D003576:Electron Transport Complex IV; D002480:Cellulase
keywords: Eisenia fetida; characterization; earthworm; endoglucanase; phylogenetic analysis