title: Age-related changes in the lateral lipid distribution in a human lens described by mass spectrometry imaging.
abstract: The lateral lipid distribution in eye lenses of three human donors were studied by matrix-assisted laser desorption ionization imaging mass spectrometry using a high mass resolution. By using exact mass measurements this study shows the relationship between the aging process and the number of lipids detected as well as between aging and the abundance of products derived from sphingomyelins by hydrolysis. Variable lipid composition was also observed in the nuclear, barrier, or cortex regions of the lens samples. This is the first study that suggests the distribution of lysolipids as a potential biomarker panel for the aging of human lens tissue.
authors: Institute of Microbiology ASCR, v.v.i., Videnska 1083, CZ 14220 Prague 4, Czech Republic. jaroslav.pol@thermofisher.com.
authors: Jaroslav
authors: J
authors: Pol
authors: Institute of Microbiology ASCR, v.v.i., Videnska 1083, CZ 14220 Prague 4, Czech Republic. helena.faltyskova@@sezuam.cz.
authors: Helena
authors: H
authors: Faltyskova
authors: Institute of Microbiology ASCR, v.v.i., Videnska 1083, CZ 14220 Prague 4, Czech Republic. Institute of Chemical Technology in Prague, Technicka 5, CZ 16228 Prague 6, Czech Republic. lukas.krasny@biomed.cas.cz.
authors: Lukas
authors: L
authors: Krasny
authors: Institute of Microbiology ASCR, v.v.i., Videnska 1083, CZ 14220 Prague 4, Czech Republic. volny@uw.edu.
authors: Michael
authors: M
authors: Volný
authors: Department of Ophthalmology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic. ondrej.vlacil@seznam.cz.
authors: Ondrej
authors: O
authors: Vlacil
authors: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 5, 779 00 Olomouc, Czech Republic. hajduchm@gmail.com.
authors: Marian
authors: M
authors: Hajduch
authors: RCPTM, Department of Analytical Chemistry, Faculty of Science, Palacky University, 17.listopadu 12, 771 46 Olomouc, Czech Republic. karel.kemr@upol.cz.
authors: Karel
authors: K
authors: Lemr
authors: Institute of Microbiology ASCR, v.v.i., Videnska 1083, CZ 14220 Prague 4, Czech Republic. vlhavlic@biomed.cas.cz.
authors: Vladimir
authors: V
authors: Havlicek