title: Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee.
abstract: This prospective acute gastroenteritis (AGE) surveillance was conducted in the inpatient and emergency room settings at a referral pediatric hospital to determine the prevalence of diarrheagenic Escherichia coli (DEC) in children <12years of age with AGE in Davidson County, Tennessee. Subjects 15 days to 11 years of age, who presented with diarrhea and/or vomiting, were enrolled. Stool specimens were processed for detection of DEC using multiplex polymerase chain reaction. From December 1, 2011, to June 30, 2012, a total of 79 (38%) out of 206 stool specimens from children with AGE tested positive for E. coli. A total of 12 (5.8%) out of 206 stool specimens from children with AGE were positive for a DEC. Eight (67%) out of these 12 were positive for enteropathogenic E. coli, and the remaining 4 were positive for enteroaggregative E. coli. DEC clinical isolates clustered with known E. coli enteropathogens according to multilocus sequencing typing.
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Monique A
authors: MA
authors: Foster
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Junaid
authors: J
authors: Iqbal
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Chengxian
authors: C
authors: Zhang
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Rendie
authors: R
authors: McHenry
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Brent E
authors: BE
authors: Cleveland
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Yesenia
authors: Y
authors: Romero-Herazo
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Chris
authors: C
authors: Fonnesbeck
authors: Centers for Disease Control and Prevention, Atlanta, GA.
authors: Daniel C
authors: DC
authors: Payne
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: James D
authors: JD
authors: Chappell
authors: Vanderbilt University School of Medicine, Nashville, TN.
authors: Natasha
authors: N
authors: Halasa
authors: Vanderbilt University School of Medicine, Nashville, TN. Electronic address: Oscar.gomez@vanderbilt.edu.
authors: Oscar G
authors: OG
authors: Gómez-Duarte
mesh_terms: D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D054308:Enteropathogenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D062665:Epidemiological Monitoring; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D015995:Prevalence; D011446:Prospective Studies; D013714:Tennessee / Q000453:epidemiology
keywords: E. coli; EAEC; EPEC; children; diarrhea; epidemiology; gastroenteritis