title: Atractylenolide I protects mice from lipopolysaccharide-induced acute lung injury.
abstract: Atractylenolide I (AO-I), one of the major bioactive components isolated from Rhizoma Atractylodes macrocephala, has been reported to have anti-inflammatory effects. In the present study, we investigated the protective effects of AO-I on acute lung injury (ALI) using LPS-induced ALI mouse model. Lung injury was assessed by histological study. Inflammatory cytokines TNF-α, IL-6 and IL-1β production were detected by ELISA. TLR4 expression and NF-κB activation were measured by western blot analysis. The results showed that treatment of AO-I significantly attenuated LPS-induced lung wet-to-dry weight ratio and MPO activity. Meanwhile, treatment of AO-I significantly inhibited the production of TNF-α, IL-6, IL-1β, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF. AO-1 could up-regulate the production of IL-10 in BALF. Besides, LPS-induced TLR4 expression and NF-κB activation were suppressed by treatment of AO-I. In conclusion, the current study suggested that AO-I protected mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation.
authors: Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Jun-liang
authors: JL
authors: Zhang
authors: Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Wei-min
authors: WM
authors: Huang
authors: Center of Pediatrics, Southern Medical University, Zhujiang Hospital, Guangzhou 510280, China. Electronic address: zengqiyi51@163.com.
authors: Qi-yi
authors: QY
authors: Zeng
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D018680:Cholinergic Antagonists / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D018680:Cholinergic Antagonists; D018836:Inflammation Mediators; D007783:Lactones; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; C424804:atractylenolide I
keywords: Acute lung injury; Atractylenolide I; LPS; NF-κB; TLR4